King Saud University Practical Pharmacology

College of Pharmacy PHL 315

Pharmacology Department Lab No. ( )

Title:

In uence of various drugs on the isolated rabbit heart (Lange do

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preparation)
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Aim of the work:

To identify the In uence of various drugs on the isolated rabbit heart
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(Lange do preparation
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Material:

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Physiological solution: Ringer Locke solution

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Apparatus: Physiography & thermostatically controlled organ bath

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Preparation: isolated rabbit heart

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- Temp.: 37° C

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Method:

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The heart is carefully isolated from a scari ed rabbit (the chest is

opened & the heart is dissected with at least 1 cm of aorta).

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The heart is then placed in a dish containing Ringer Locke solution and
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squeezed gently several times to remove the blood.

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The aorta is tied to a cannula attached to the bottom of perfusion tube.

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A hook is xed to the apex of the heart & attached to the mayo graph

by a thread.

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The heart is perfused with oxygenated Ringer Locke at constant temp.

of 37° C. Ø The drugs are injected through the rubber cap on top of
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cannula.

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Results:
Title
In uence of various drugs on the isolated rabbit heart (Lange do
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preparation)
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Curve:

Key:
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 Tabulate the results & record the observation

Drug / volume Observation Comment

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Drug / volume Observation Comment
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