[00:00:00.

000] - Speaker 1
Good afternoon to everyone. Myself, Dr. Bhatnilal Kalal, Department of Chemistry, IPS Academy, Indore.
Now, we are going to perform estimation of caffeine experimentally. For this, first of all, we prepare 200
ppm caffeine solution in water, 250 mL that is of concentration of 200 ppm. This is the stock solution. This
solution is used for preparing different dilution. Here, we are going to make four dilution that is 25 ppm, 50
ppm, 75 ppm, and 100 PPM. The amount that is required to form this solution, we calculate by formula n
1 V 1 equal to n 2 V2. For 25 ppm, we required 6.5 mL of 200 ppm caffeine. For 50 mL, we required 12.5
mL. For 75 mL, we required 18.75 mL. And for 100 PPM solution, we required stroke solution of 25 mL.
That our solution are already made so that experiment goes easily. Now, these solution are used, these
dilution are used to perform experiment. We have to take 1 m l of caffeine solution, caffeine dilution,
okay? And 5 m l of dichloromethan. Both these solution are put into this separating funnel and shake for
five minutes, okay? After that, after that, we keep it for some time so that both the layer of caffeine
solution and dichloromethan gets separated.

[00:02:41.540] - Speaker 1
After that, in both layer, dichloromethane as heavier, its density is higher than water, that it goes, that it lie
in the lower layer. We separate this lower layer and collect in this iodine flux. Again, water layer remain in
the separating funnel. Now, again, we take 5 mls of dichloromethan in the separating funnel and again
shake for 5 minutes. And again, we separate the dichloromethan layer. We perform this experiment with
all these dilution. Now, I am going to demonstrate you the similar procedure with the soft drink. So let us
start the experiment with excipient with soft drink. So I am going to first take one mL of soft drink. First of
all, I take this soft drink into this beaker. Here, I am going to take one ml of soft drink Take care that no
bubble formation take place in this pipette. Now, I have taken one mL of soft drink in separating funnel. I
am repeating this step again as the valve was open, so ensure that the valve of separating funnel close
before taking soft drink. With soft drink, there is a problem that there is take place of bubble formation in
capillary pipette. So be careful that no bubble formation take place in the pipette.

[00:06:25.180] - Speaker 1
Here, I have taken 1 mL of soft drink in separating funnel. Now, take 5 mL of dichloromethan. I have
taken 5 mL of dichloromethan in separating funnel. Now put stopper on the mouth of separating funnel
and shake the content of separating funnel for five minutes.