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  • Polymerase Chain Reaction

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    Dinarherlina 235
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    PCR is a technique that amplifies a single DNA sample millions of times to generate enough material for analysis. It works by denaturing DNA strands, adding primers that attach to the strands, and using DNA polymerase to synthesize new DNA strands, repeating this process to exponentially increase the number of DNA copies. The amplified DNA can then be analyzed through electrophoresis.

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    POLYMERASE CHAIN REACTION

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    PCR is a technique that amplifies a single DNA sample millions of times to generate enough material for analysis. It works by denaturing DNA strands, adding primers that attach to the strands, and using DNA polymerase to synthesize new DNA strands, repeating this process to exponentially increase the number of DNA copies. The amplified DNA can then be analyzed through electrophoresis.

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    9 views3 pages

    Polymerase Chain Reaction

    Uploaded by

    Dinarherlina 235
    PCR is a technique that amplifies a single DNA sample millions of times to generate enough material for analysis. It works by denaturing DNA strands, adding primers that attach to the strands, and using DNA polymerase to synthesize new DNA strands, repeating this process to exponentially increase the number of DNA copies. The amplified DNA can then be analyzed through electrophoresis.

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    © All Rights Reserved
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    of 3

    POLYMERASE CHAIN REACTION

    PCR is a technique that is used to amplify one sample of a)..…… thousands of times over to create a
    large enough b)……. Sample for extensive c)……. It is d)…… amplification of DNA.

    What is needed?
    1. …………………… has functions …………………
    2. …………………… has functions …………………
    3. …………………… has functions …………………
    4. …………………… has functions …………………
    5. …………………… has functions …………………
    6. …………………… has functions …………………

    The Process of PCR


    Step 1 ……………………………………… of DNA, means to separate the DNA strands into 2 sepatare
    stands. This involves heating the DNA samples up to ……………………….!
    Step 2 ……………………………………… the DNA means to add. In this step 2 ………………………… are
    added to the 2 separated DNA (1 on each strand). The temperature is cooled to ………………,
    this help the primers bind to the DNA. The primers is an signal to a ………… where to start
    …………… (making) new DNA.
    Step 3 ………………………………………. Of DNA. New DNA created. 2 ………………….. molecules
    attached to the 2 …………………. On the 2 DNA strands and move along the strand. As they
    move along they create new …………………. DNA. Temperature goes up to………………..
    ELEKTROFORESIS

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