Professional Documents
Culture Documents
(Engineering Edition)
URIT8021A
Automatic Chemistry Analyzer
V1.0
Catalogue
Copyright ....................................................................................................................................................................1
PREFACE......................................................................................................................................................................2
CHAPTER 1 INSTRUMENT INSTRUCTION .......................................................................................................................3
1.1 Brief Intrduction .......................................................................................................................................................... 3
1.2 Intended Use................................................................................................................................................................ 3
1.3 Structure ...................................................................................................................................................................... 3
1.4 Function of Main Part.................................................................................................................................................. 5
CHAPTER 2 PERFORMACE AND TEST FLOW...................................................................................................................6
2.1 Technical Parameter..................................................................................................................................................... 6
2.2 Operational Environment ............................................................................................................................................ 6
2.3 Test Process ................................................................................................................................................................. 7
2.3.1 Typical Test FlowChart...................................................................................................................................... 7
2.3.2 Test Process Instruction .................................................................................................................................... 8
CHAPTER 3 INSTALLATION AND DEBUGGING............................................................................................................... 12
3.2 Installation ................................................................................................................................................................. 14
3.2.1 Instrument Inspection .................................................................................................................................... 14
3.2.2 Installation ...................................................................................................................................................... 15
3.2.3 Environmental Requirements ......................................................................................................................... 15
3.2.4 Location Requirements................................................................................................................................... 16
3.2.5 Power Requirements ...................................................................................................................................... 16
3.2.6 Instrument Connection................................................................................................................................... 17
3.3 Installation of Aspirating probe and Stirrer ............................................................................................................... 18
3.3.1 Aspirating probe Installation........................................................................................................................... 18
3.3.2 Stirrer Installation ........................................................................................................................................... 21
3.4 Saftware Installation.................................................................................................................................................. 22
3.5 Instrument Adjustment .............................................................................................................................................. 26
3.5.1 Parameter Setup............................................................................................................................................. 26
3.5.2 Setup of Motor Speed..................................................................................................................................... 30
3.5.3 Parameter Setup of Liquid Path...................................................................................................................... 31
3.5.4 Setup of Initial Position................................................................................................................................... 33
3.5.5 Adjusting Position Parameter to Washing Pool .............................................................................................. 38
3.5.6 Adjusting Position of Reagent /Sample Tray ................................................................................................... 40
3.5.7 Debugging of Optical Path .............................................................................................................................. 41
CHAPTER 4 MECHANICAL UNIT MODULE .................................................................................................................... 44
4.1 Shell and Structure of the Machine ........................................................................................................................... 44
4.1.1 Shell ................................................................................................................................................................ 44
4.1.2 Disassembly and Instructure .......................................................................................................................... 45
4.2 Aspirating Unit .......................................................................................................................................................... 50
4.2.1 Function.......................................................................................................................................................... 50
4.2.2 Structure ......................................................................................................................................................... 50
4.2.3 Aspirating probe Installation........................................................................................................................... 52
4.3 Stirrer Unit................................................................................................................................................................. 52
4.3.1 Function.......................................................................................................................................................... 52
4.3.2 Structure ......................................................................................................................................................... 53
4.3.3 Stirrer Installation ........................................................................................................................................... 54
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URIT‐8021A Service Manual
Copyright
Copyright: URIT Medical Electronic Co., Ltd.
URIT Medical Electronic Co., Ltd. owns the intellectural property rights to this service manual. No part of this
manual may be reproduced, stored or transmitted in any form, or by any means without the express written
permission of URIT.
Customer Service
URIT Medical Electronic Co., Ltd.
ADD: No.3 Fuhe Alley, Zhonghua Road, Guilin, Guangxi 541001, PR China
Customer Service hotline: 86 773 2288566 86 773 2825742
400 Service hotline: 400 727 2288
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URIT‐8021A Service Manual
PREFACE
This document is the service manual for URIT8021A discrete random access chemistry analyzer. It
describes the structure, operation, maintenance and troubleshooting concerning the instrument in details.
Users should read carefully the manual and get special training before operating to guarantee instrument
precision, normal operation and personal safety.
Sign Illustration
Meaning of the signs used in the URIT8021A is as following.
Caution. Refer to the
Caution. Electric shock
accompanying document
Ground Power on
In vitro diagnostic
Power off
medical device
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URIT8021A is clinical chemistry instrument with the characteristics of open, fullautomatic, discrete, STAT
priority and computercontrolled. URIT8021A is intended for use in conjunction with reagents to measure
quantitatively certain chemical items in serum, urine and cerebrospinal fluid. Please read the operating
manual carefully before using since it is a high sophisticated instrument.
1) Work Unit consists of optical unit, mechanical operation unit, liquid path control unit, hardware circuit
unit and operating unit.
2) Mechanical operation unit consists of aspirating system, stirring system and eightphase washing
system.aspirating system includes sample tray, reagent tray, aspirating mechanism, aspirating probe
(the inner core of syringe is made of ceramic which aspirating accurately and maintenancefree ) and
sample probe wash station.The sample tray has 45 positions, which contains 35 rountine positions, 5
standard positions, 3 QC positions and 2 STAT positions. Tube or sample cup could be placed on
routine position. The reagent tray could place the reagent bottle in different specifications. The optical
unit is a whole sealing, statics, array and rear spectrophotometry optical system, which includes 120
cuvettes, highresolution filter and halogen light.
3) The operating unit is a external computer (CPU 2.0 GHz or above, 16XDVD drives, memory: 1GB or
above) , the application software should be setup under the Windows XP operation system.
4) Liquid path control unit consist of vacuum pump, solenoid valve, syringe, rinse system and tube system.
5) The instrument is easy to operate. The layout of the screen menu is reasonable, name is simple. Such
as testing parameter setup, patient’s information input, qualitycontrol, reagent, data query, standard,
running test and hardware parameter. After setting, put the sample and reagent to the instrument and
begin to analyze. Print out the result by the external printer at last.
The instrument is only for professional, in vitro use in hospitals, clinics and laboratories.
CAUTION
Please contact the reagent manufacturer or distributor if some samples may not applicable to
analytical test according to the test parameter and reagent.
1.3 Structure
The instrument structure mainly consists of optical system, reaction system, rinse system, sample system,
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The instrument is mainly composed of analyzer, computer and printer. Printer is optional accessory.
The analyzer mianly consists of reagent tray, sample tray, reaction tray, aspirating system, stirring system,
optical system, rinse system, liquid path system and hardware circuit.
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Main workflow:
1) Mechanism unit startup the initialization progress
2) The eightstep wash mechanism washing the cuvettes automatically (No this step, if you do not select
the clean before test option).
3) A washed and clean cuvette rotated to reagent aspirating position.
4) The reagent tray convey first reagent to aspirating position. The aspirating probe (aspirating mechanism)
turn to the aspirating position and move down to absorb first reagent, then deliver it to cuvette.
5) First reagent in the cuvette preheated several cycles and then moved to sample aspirating position.
6) The sample tray conveys the sample to aspirating position. The aspirating probe (aspirating mechanism)
turn to the aspirating position and move down to absorb sample, then deliver it to the cuvette.
7) Cuvette which with reagent and sample solution moved to stirring position to stir.
a) Constant speed mode: After fixed cycle, cuvette rotated to the relative position, the reagent tray
convey second reagent to aspirating position. The aspirating probe (aspirating mechanism) turn to
the aspirating position and move down to absorb second reagent, then deliver it to the cuvette.
b) Mixed mode: After arrive the incubate time of first reagent, cuvette rotated to the relative position,
the reagent tray convey second reagent to aspirating position. The aspirating probe (aspirating
mechanism) turn to the aspirating position and move down to absorb second reagent, then deliver it
to the cuvette.
9) Cuvette moved to stirring position to stir after the addiction of second reagent.
10) During each cycle, each of the cuvettes transmitted to the photometric position (the light source position)
for automatic signal collecting and absorbance calculation.
11) Eightstep washing mechanism washes the cuvettes automatically after finishing the test.
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The meaning of the numbers in the figure 22 is the same as the figure 21, please refer to 2.3.1.1
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Please comply with the following rules for safety and active use.
Preventing Injury
Please observe the following precautions for preventing injury.
CAUTION
1) While the instrument is in motion, DO NOT touch the moving parts, such as sample probe,
reagent probe and stirrer, etc. Otherwise, you may be injured.
2) Before lamp replacement, turn off the power switch and wait until the lamp is cooled down.
Otherwise, you may receive burns.
CAUTION
Some reagents are strong acid or alkaline. Please use them carefully avoiding direct contact. If
the reagent sticks to the human body, immediately wash it off with water and soap. If the
reagent splashes into eyes accidentally, wash it off with water and consult an oculist.
Disposing Wastes
Please comply with the following matters when treat the wastes in order to avoid personal injury and protect
environment.
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Biological Hazard
Some substances contained in reagent, control, calibrators and waste are subject to regulations
of contamination and disposal. Dispose of the waste in accordance with your local or national
rule for biohazard waste disposal.
Operational Environment
CAUTION
1) Please install the instrument according to the specified installed instruction in the manual.
Otherwise, the results may not reliable even may cause system damage.
2) Please contact URIT if system state changed is necessary.
Systematic Usage
CAUTION
1) The operator must receive training before operating the instrument. Please follow the
instruction of the manual to operate. Improper operation may cause personal injury, system
damage and improper result.
2) Please make a calibration and qualitycontrol test when use the system for the first time to
ensure it can be used normally.
3) A qualitycontrol test must be done when use the system. Otherwise, the reliability of the
result could not be guaranteed.
4) The communication interface of analytic part is set to connect with the communication
interface of operation part. Please use the cables of URIT for connecting.
5) The computer should be for the instrument exclusive use. DO NOT run any other software
with the computer while it is connected with the instrument. Inappropriate manner may result
computer virus infection.
Other Cautions
CAUTION
1) DO NOT touch the keyboard, indicator and mouse when your hands is wet, also includes the
chemistry.
2) Check samples for contamination (dust, or fibrinogen) and air bubble before analyses.
3) To make periodic maintenance, test and replacing according to the manual for getting the
exact result.
4) For replacements of major parts, such as light source lamp, aspirating probe, reaction cuvette
etc, please contact URIT.
5) For settings of sample volume, reagent volume, wavelength, standard values etc, please
refer to the instruction in reagent kit as well as this operating manual. Take note of checking
the quality of distilled water and detergent, calibration results, control results, and sample
results. Make sure there is no air bubble in the liquid paths.
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3.2 Installation
WARNING
The URIT8021A analyzer should be installed only by technicians of or authorized by URIT.
Only the technicians of or authorized by URIT can perform the installation of URIT8021A, users shall make
preparation for satisfying the installation requirements in accordance with this manual before installation. If
relocation is necessary, please contact your local distributor or URIT.
Figure 31
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2) Take out the analyzer and put on the cabinet. See figure 32
Figure 32
3) Open the package of liquid path and take out.
4) If any loss or damage exists, contact the distributor or manufacturer immediately.
3.2.2 Installation
The instrument is high sophisticated thus proper installation is very important to its performance. User
should guarantee the environment and electrical condition are comply with the recommended conditions.
Provide a distance of 50cm at least for each side for operating and maintaining.
The instrument installation layout is below. Surrounding distance is the recommended maintenance space.
Measure: cm
Instrument Dimension: 89 73 112cm (L W H)
Dimension of Operating Board (Only for reference): 70 50 80cm (L W H)
The following power must be prepared; switchboard should be located within 10m.
1) Power
Voltage: AC 230V, 50/60Hz
2) Grounding
Using threepin power plug
3) Plug board
A 15A output plug board with more than three 5A sockets. Heavyduty devices should not share the plug
board with the instrument, such as refrigerator, air conditioner etc.
4) 3 core power cable cat is using; the type of wire and plug is depended on voltage.
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CAUTION
Make sure the instrument is grounded properly. Poor grounding may cause bad effects on test
result and even damage to the instrument.
To connect the liquid path tubes according to the marks on tubes and the silkprinting on the rear of the
instrument.
CAUTION
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If you drain the waste solution into sewer directly without waste barrel, please short connecting
the interface of waste solution BNC to prevent the alarm that waste solution is full. Otherwise,
the liquid level sensing buzzer will alarm.
In order to prevent damage during transportation, aspirating probe and stirrer are disassembled and packed
individually, then reinstalled and debugged after arrive the destination.
1) Uplift the arm of the aspirating probe to the highest position and then rotate it to the above of reagent
/sample tray.
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Figure 36
Figure 37
3) Remove the retaining screw, insert the aspirating probe downwards into the positioning hole and tighten
the retaining screw, then connect the liquid level sensing connector on the circuit board. See as the
figure 38.
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Figure 38
NOTE
1) Keep the spring carefully, when remove the retaining screw.
2) Uplift sampling arm to the highest point before installing aspirating probe. Make sure the
anticollision plate installed between anticollision optocouple and spring pressed on
location plate.
Figure 39
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Figure 310
NOTE
Keep the flat washers and spring washers carefully to prevent fall into the instrument, when
remove the restaining screws of stirrer.
Figure 311
3) Remove the retaining screws; insert it downwards into the positioning hole, then tighen the restaining
screw.
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Figure 312
NOTE
The positioning hole for adjusting the front and rear position of stirrer. Two screws on the
adjusting cover do not need to fix tigh before adjusting the initial position.
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Figure 313
2) Click Next, the dialog box to choose the installation location is shown as figure 314.
Figure 314
3) Click Next, the message box is displayed as figure 315.
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Figure 315
4) Click Next, the message box is displayed as figure 316 to choose whether create a desktop icon or not.
Figure 316
5) Click Next, the message box is shown as figure 317. Then click Install botton to perform the installation
process.
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Figure 317
6) The message box will be shown as the figure 318 after complete the installation, then click Finish to
exit.
Figure 318
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Figure 319
Send the User ID to AfterSale Service to obtain the License Number, and then input the number in blank
Figure 320
Click OK, then close the software and restart according to prompt, the installation is completed.
This section mainly introduces the instrument adjustment method. Please make sure the correct connection
of piping and wiring.
1) Doubleclick , then select Server for adjusting and input the password 3112772 into the main
interface.
2) Turn on the red power switch, then green testing switch. And check that if abnormal for instrument.
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Figure 321
4) The instrument has been adjusted and the parameters restoraged in the main board before leaving
factory. Click
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CAUTION
l Cuvette :
Cuv.1: Cuv.1 will move over to the initial position of reaction tray.
l Reset:
Reset: It does affect any operation if not perform resetting after turn on the power.
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l Fine Adjustment:
Perform fine adjustment for parameters of selected item.
There are four commands up, down, right and left to adjust the parameter of selected item.
l Test:
It is not available if the button of Test display grey color, click the Adjust button to operate it.
CAUTION
The buttons will come into action immediately after clicking. Make sure that all the mechanism is
in Arm raised state to prevent probe collision.
1) Reaction cuvette: The corresponding cuvette will rotate to the reagent aspirating position.
2) S. position: The corresponding sample cup will rotate to the sample aspirating position.
3) R. position: The corresponding reagent bottle will rotate to the reagent aspirating position.
4) Reset: The corresponding sample or reagent injection pump will rotate to the initial position.
5) Sam. In: The sample injection pump will aspirate quantitative volume of sample, measured by ul.
6) Reg. In: The reagent injection pump will aspirate quantitative volume of reagent , measured by ul.
7) Out: The sample or reagent injection pump will dispense the volume aspirated in the previous step.
8) S.probe fill water: the liquid path is open for washing the inner wall of sample probe, take times as unit.
9) Wash arm fill water: the liquid path is open for washing the inner wall of reagent probe, take times as
unit.
10) Running gear: Running in the moving component of reaction tray.
11) Stop: Stop running gear.
l Functional Buttons:
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Enter instrument parameter interface and click , the dialog box shown as figure 223 will pop
up
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NOTE
1) The actual value of each parameter is consist of two value in the case, such as 250 and
247, 250 represent big unit and 247 represent small unit(fine adjustment).The bigger the
value is , the faster the speed of motor is.
2) To setup the parameters in this interface according to Parameter Table.
3) The maximum speed is 255.
Figure 323
2) Check if the value of each parameter is consistent with the value in Parameter Table.
3) Each serial number in the interface is corresponded with the pump, valve or motor. The corresponding
value is the opening time of pump, valve or motor.
4) The right part of the interface is the function section; the unit of value is given in the bracket.
5) It is available for testing whether these components are connected properly. Input the corresponding
serial number and click Switch On, the corresponding pump, valve and motor will start to work. See
figure 324.
Figure 324
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NOTE
1) Take ms as unit for parameter which is not indicated unit
2) The serial number in front of each parameter represents the corresponding number of
motor.
3) Please consult the technician before performing fine adjustment of parameter
4) Click SAVE to save the data after adjusting.
CAUTION
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Figure 328
Figure 329
click . Perform fine adjustment (the range of fine adjustment is 1 to 5) and observe
the position of aspirating probe in cuv.1. If it is proper, down the aspirating probe till the tip touches the
bottom of cuvette through gently pressing the arm of aspirating probe.
l When the tip is near the bottom of cuvette, please operate the align function step by step. The region of
fine adjustment shown as figure 330:
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Figure 331
click , perform fine adjustment (the range of fine adjustment is 1 to 5). Upward
moving 2 to 3 step when the stirrer reaches the bottom of cuvette.
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click , perform fine adjustment (the range of fine adjustment is 1 to 5), descending
step by step. The requirement of adjusting washing hand as following:
a) The first to sixth porbe’s height is the same, the seventh and eighth are the same and deeper 1to 2
mm than the former.
b) scrap block is not permitted to touch the wall of cuvette(perform fine adjustment of radian or turn
rotate probes), see figure 332:
Figure 332
c) The spring anticollision assembly of seventh and eighth probes raised 1 to 2mm (2 to 3 steps), and
others do not raised. See figure 333:
Figure 333
Scrap block is not permitted to touch the wall of cuvette during the adjusting course. Adjusting the
position of washing hand must be done if the scrape block touch the wall and diverge the cuvette. (the
position of washing hand has been adjusted before delivery, it is not necessary to adjust on the scene).
The adjusting methods are as follows: keep the position of reaction tray and loose four locking screws.
Then adjusting whole position of washing hand to make sure scrape block is above the centre of cuvette
35.
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Figure 334
click and make aspirating probe above the washing pool. Perform fine adjustment
to adjust left/right of aspirating probe to make sure it is above the centre of washing pool.
to 5) to make the inclined part of sampling part and up edge of inner washing cup at same horizontal line.
See figure 335:
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Figure 335
2) Stirrer to washing pool:
Figure 336
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l The position of aspirating probe to reagent tray could be adjusted after performing the above processes.
If a large deviation still exist, the initial position of reagent tray should be adjusted. The adjusting
methods are as follows:
Loose three locking screws on the reagent tray bracket, and then move the reagent tray to make sure
the aspirating probe at the above of the centre of No.1 reagent position. See figure 337:
NOTE: It is not necessary to adjust the initial position of reagent tray since it has been done before
delivery.
Figure 337
click to adjust reagent probe above the centre of reagent bottle. The aspirating probe
could move down to the bottom of reagent bottle and keep the probe do not collide reagent bottle since
vertical of inner and outer reagent bottle are the same.
click . Adjust aspirating probe down to a position in the sample cup which is easy to
1) Wait for at least 30 minutes to make sure the light source is stable after power on. Then click
to enter cuvette signal interface, click , 120 cuvettes will be added water by
washing hand.
2) Click in the instrument parameter interface to make the reaction tray move to the
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Figure 338
3) Adjusting the potentiometers on the signal board to make the A/D value of corresponding wavelength is
in the range of 56000 3000. See figure 339
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Figure 339
4) Click to enter the interface shown as figur340. Then click , then the
interface will show the A/D vaule of 120 cuvettes after reaction tray stop.
Figure 340
5) Observe the A/D value and absorbance value. If the A/D value or absorbance is out of range, please
replace the corresponding cuvette to meet the requirements. Then click SAVE, and the debugging of
optical path is complete.
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This section introduces the internal structure of the machine, shell’s composition, remove and installation.
4.1.1 Shell
The shell of URIT8021A is mainly composed of the protective cover, panel, left/right cover plate, front shell,
rear baffle, rear cover plate and cabinet. See figure 42:
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Figure 42(a)
Figure 42(b)
It’s recommended that the analyzer should be repaired by professional personnel if the malfunction cannot
be solved according to the adjustment of software and routine maintenance. In case replacing a component
is needed, please following the steps:
1) Turn off the testing power switch.
2) Turn off the mian power switch.
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3) Remove the end of gas springs, then take down two hinges to remove the upper cover.(In general the
upper cover don’t need to remove)
4) Loose the six hexagonal socket head cap screws on the left and right sides of the front shell, and 5
screws on the front of panel, then pull out the front shell. Drive board, optical box, injection pump could
be seen. Seen figure 43:
Figure 43
5) The installation plate fixed in the frame by two hinges and a socket head cap screw. Loose the retaining
screw and open the installation board. Main board, power board and terminal board are installed on the
back of installation board. See figure 44:
Figure 44
6) The rear baffle is used for protecting liquid path. See figure 45:
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Figure 45
The liquid path could be seen when removing the rear baffle. See figure 46:
Figure 46
1Pump for supplying distilled water 4Distilled water tank
2Pump forsupplying detergent 5Drain waste solution negative pressure tank
3Pump for draining waste solution
7) After removing rear baffle, loose the screws on the rear cover plate, then down open the rear cover plate,
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Figure 47
8) Loose four M4 10 screws to remove the left cover plate. Then reagent tray refrigeration assembly(left),
reagent/sample tray, reagent temperature sensor could be seen, see figure 47:
Figure 48
9) Remove the right cover plate, reaction, power supply box, optical box and a part of washing hand, see
figure 49:
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Figure 49
10) The panel consists of four panels and two tray covers, shown as the figure 410. The disassembly
sequence of panel is: reaction tray cover reagent /sample tray cover arc middle panel B arc
middle panel A arc left panel arc right panel.
NOTE
Do not touch the aspirating probe and stirrer when disassembly panels.
Figure 410
Figure 411
4.2.1 Function
Sample probe and reagent probe share a aspirating probe in aspirating unit. The mainly function is aspirate
the sample from sample cup (tube) to inject into cuvette and the operation of reagent is the same as sample.
And it’s also provide liquid level sensing, aspirating probe anticollision, with the amount of tracking,
mechanical limit, power off protection etc.
4.2.2 Structure
Aspirating unit consists of drive mechanism and sampling arm. See figure 412:
Drive mechanism used for supporting sampling arm and making aspirating probe to vertical and horizontal
rotation. The drive mechanism is composed of vertical and rotary movement mechanism which are includes
stepping motor, synchronization belt and synchromesh gear, to perform the movement of probe by spline.
Sampling arm is composed of aspirating probe, liquid level sensing board, sampling arm cover and retaining
screw of aspirating probe, which supported and connected by sampling arm bracket. See figure 413:
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Figure 412
Figure 413
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Figure 414
1) Remove the sampling arm cover, and then loose the retaining screw and spring to take down the
aspirating probe since it’s fixed on the sampling arm bracket.
2) Loose two screws that fixed on the spline to take out the sampling arm bracket.
3) Sampling mechanism installed on baseboard by four inner hexagonal screws, loose the screws to take it
out.
4) The installation steps are contrary to disassembly.
CAUTION
1) Checks the movement of aspirating probe whether is smooth or not after strengthen the
retaining screw, if not, loose the retaining screw to adjust.
2) Keep the surface of aspirating probe clean when disassemble the mechanism.
3) Disconnect the relative electric circuit and liquid path before disassembling the mechanism.
4.3.1 Function
The stirrer has the functions of mixing the sample and reagent in cuvette, mechanical limit, Power off
protection.
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4.3.2 Structure
Stirrer unit is composed of drive mechanism and stirring arm. The main structure is shown as figure 415.
Drive mechanism is used for supporting stirring arm and making stirrer to vertical and horizontal rotation.The
drive mechanism is composed of vertical and rotary movement mechanism which are includes stepping
motor, synchronization belt and synchromesh gear, to perform the movement of probe by spline.
Stirring arm is composed of stirring assembly and stirring arm cover, which supported and connected by
stirring arm bracket. See figure 415:
Figure 415
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Figure 416
1) The stirrer assembly consists of stirring motor, stirrer and micro adjustment plate, and fixed on the
stirring bracket by two screws. Remove the stirring arm cover, and then loose two retaining screws on
the micro motor adjustment plate to take down the stirrer.
2) Loose two screws that fixed on the spline to take out the stirring arm bracket.
3) Stirrer mechanism installed on baseboard by four inner hexagonal screws, loose the screws to take it
out.
4) The installation steps are contrary to disassembly.
CAUTION
1) When installing stirrer assembly, please downwards into the position hole.
2) Keep the surface of stirrer clean when disassembling the stirrer assembly.
3) Disconnect the relative electric circuit before disassembling the mechanism.
4.4.1 Function
Reagent/sample tray is a container to load sample and reagent, and transfer accurately them to
corresponding position in sequence. The mianly function as follows:
1) Load reagent/sample: The sample cup (tube) with sample placed on the sample tray, and reagent
bottle with reagent placed in the reagent tray.
2) Rotate in sequence: Reagent/sample tray rotates in the sequence that setted by software, transfers
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4.4.2 Instructure
Reagent/sample tray unit is divided into sample tray bracket, reagent tray bracket, reagent/sample tray drive
assembly, refrigeration module and reagent/sample tray thermostat assembly. The description of structure
as following:
1) Reagent/sample tray bracket fixed on reagent/sample drive assembly.
5) Sample tray bracket: load sample cup (tube), single ring, 45 sample positions. Controlled to rotate in
sequence by sample tray assembly.
6) Reagent tray bracket: load reagent bottle, includes outer and inner rings, each ring contains 30 positions,
and 60 reagent positions in total are provided by two rings. Controlled to rotate in sequence by reagent
tray assembly.
8) Refrigeration module: for reagent refrigeration to make reagent meet the requirement of refrigeration
temperature, and keep stability of reagent. It’s consists of two fancooled refrigeration assemblies and
reagent/sample tray drive assembly.
9) Reagent/sample tray thermostat assembly: Protect the reagent/sample tray and keep the temperature
of reagent tray.
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Figure 417 Sample tray bracket Figure 418 Reagent tray bracket
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Figure 420
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Figure 422
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Figure 423
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4.5.1 Function
Reaction tray is holds cuvettes and provides a steady environment for biochemical test, which transmits
cuvettes to corresponding position in the sequence setted by software. It’s also cooperates with aspirating
probe, stirrer, washing hand and optical system to work.
4.5.2 Structure
Reaction tray is composed of cuvette holder assembly, constanttemperature incubation slot and reaction
tray drive assembly.
Cuvette holder assembly: it holds cuvette and consists of six groups of cuvette holders, 120 cuvettes and a
reaction tray cover. Reaction tray cover is installed on reaction tray drive assembly by nut cap.
Constanttemperature incubation slot: it’s installed on fitting seat by four inner hexagonal screws. Heating
strip is sealed in slot by cement gel, which connects to socket by a temperature switch.
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Reaction tray drive assembly: accurately control the movement of reaction tray. It’s composed of stepping
motor, optocoupler, coded disc, helical gear, bearing and reaction tray baseboard.
1) Loose the fitting nuts on a group of cuvette holder, then take out the holder and cuvettes to vacate a
place. Manually rotate the reaction tray to make washing hand is above this place.
2) Loose nut cap to take out reaction tray cover and cuvette holders.
3) Remove the screws on constanttemperature incubation slot; disconnect the circuit of temperature
switch, then take out constanttemperature incubation slot.
4) Loose the screws on baseboard to take out reaction tray drive assembly.
5) Installation steps are contrary to disassembly.
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4.6.1 Function
The function of washing hand is vertical moving to clean the cuvettes automatically.
4.6.2 Structure
Washing hand unit is composed of washing hand and drive assembly. Washing hand is fixed on the drive
assembly by metal thumbscrew and drive assembly fixed on baseboard.
Figure 426
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4.7.1 Function
Photoelectric detection unit is one of the centre units which directly determines the accuracy and precision of
analyze. It’s to produce light, split light, receive signal and convert light signal into electrical signal.
Optional wavelength: 340nm, 405nm, 450nm, 492nm, 510nm, 546nm, 578nm, 630nm, 700nm, 800nm
Accuracy of wavelength: 2nm
Linearity range: 0 to 3.0Abs
Resolution: 0.0001Abs
Light source: halogen lamp, 12V/20W.
4.7.2 Structure
The photoelectric detection unit is a whole sealing, statics, array and rear spectrophotometry optical system.
It’s mainly composed of light source holderassembly and optical box assembly. See as figure 427:
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There are ten potentiometers in the right open slot of optical box for adjusting bright value. Ten
potentiometers are corresponded to 10 wavelengths respectively from up to down, they are 340nm, 405nm,
450nm, 492nm, 510nm, 546nm, 578nm, 630nm, 700nm and 800nm.
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Figure 432
The injection pump (500 ) located in the front of analyzer, with the left solenoid valve and aspirating probe
composes sampling assembly that fixed on the instrument by a mounting plate. The disassembly steps are
as following:
1) Screw out the black connectors on the injection pump.
2) Loose socket head cap screws to take out the injection pump.
3) The installation steps are contrary to disassembly.
CAUTION
1) Two black connectors should be connected to corresponding interfaces.The connector
which connects with solenoid valve is connected to the interface in the middle part of
injection pump, and the other one connected to the interface on the top.
2) There is a green collar inside the black connector to connect the Teflon pipe. Ensure the
mouth of teflon pipe parallel to the face of collar and should not extend or retract, otherwise
the leakage of aspirating probe may be occured.
Figure 434
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5.1 Function
1) Liquid path system is divided into two parts: sample aspirating and washing.
2) The part of aspirating sample consists of a aspirating probe, injection pump (500 ), solenoid valve and
stirrer, which provided by distilled water tank. It flow through solenoid valve and open to the 500
reagent injection pump then open to aspirating probe to wash the inner wall of probe.
3) The part of washing includes distilled water supply and waste solution drainage.
l Distilled water is draw to distilled water tank, then generate positive pressure. If the pressure is out of
the range that pressure switch provides, the pump and valve will be turned on to supply water.
l Drain waste solution pipeline is divided into two parts: motive power drainage and gravity drainage.
Aspirating probe washing pool and stirrer washing pool waste solution drainage, and reagent
condensated water drainage are gravity drainage that draw waste by it’s gravity. All waste solution
drainages are motive power drainage except washing pool in the instrument. Waste solution pump
generates motive power to make waste solution vacuum tank into vacuum negative pressure. All
pipelines are controlled by solenoid valve. When the pipline to draw waste solution into waste solution
negative pressure tank, the corresponding solenoid valve is turned on. Finally, waste pump drain the
waste solution from instrument.
4) Washing pool for washing the inner and outer walls of aspirating probe, and stirrer with distilled water,
then the waste will be draw into waste solution barrel.
5) Washing hand is to clean cuvettes automatically which composed of eight groups of probe, the front six
groups are doubleprobe, and the other two groups are signalprobe.
l The first group is detergent probe, the short probe is for injecting detergent controlled by individual
motive pump and valve, the long probe is for drawing waste solution to waste negative pressure tank by
vacuum pump.
l The groups of second to sixth are the distilled water probe. The long probes is for drawing waste
solution to waste pipe by vacuum pump; Five short probes is injecting distilled water, the motive power
is provided by water adding vacuum pump, a liquid separate box is used for separating distilled water
into five ways. Furthermore, a pump back system is setted for preventing liquid dropping from short
probes. A pump back solenoid valve control the system, both ends of solenoid valve connect with
distilled water injecting pipeline and waste solution negative pressure tank respectively. When the
solenoid valve is open, both ends is connected, the excess distilled water in probe will be draw by
negative pressure.
l The long probes of first to seventh groups drain distilled water and collect to a pipeline by a solenoid
valve.
l The eighth group is the drier probe which controlled by a individual solenoid valve.
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Figure 51
6) Washing pool liquid path is divided into two paths and each of them controlled by a solenoid valve to
provide distilled water for washing pool to rinse aspirating probe and stirrer respectively. And all waste
will be collected to a pipeline and then flow to waste solution barrel by it’s own gravity.
7) There is an exhaust valve to keep the gas and liquid in distilled water tank balance, and drain excess
gas.
8) The waste solution from washing pool and condensated water from reagent tray are drained by it’s own
gravity.
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Figure 52
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6.1 Summary
This chapter mainly introduces the connecting methods and principles of circuit and function of each circuit
board, etc.
4 Power board Supply power to each circuit board of lower position machine.
Transfer board of Receiving signal of Drive board and control onoff of each pump and
5
pump and valve valve.
The function of signal amplify board is to collect light signals, adjust
6 Signal amplify board and do A/D signal transition, then supply the transferred signal to main
board.
liquid level sensing Providing function of liquid level sensing and anticollision for
7 board of asipirating aspirating probe
probes
Mainly receiving driven signal of controlling motor and transfer
optocoupler signal to Drive board. (Includes transfer board of
Transfer board of
8 aspirating mechanism, transfer board of reagent/sample tray, transfer
optocoupler of motor
board of injection pump, transfer board of stirring mechanism, transfer
board of washing hand and reaction tray.)
Transfer board of Supply power to transfer light.
9
light
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Figure 63 Transfer board of pump and valuve, optocoupler transfer board of motor
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Power of
5V power main board
【DY5V】 (PO1)
4.096V
test point
DB.ZG
The connector
for connecting
signal amplifer
board.【DB.ZG】
Waste
solution
3.3V test BNC
(JP9)
point 【FBNC】
5V test Detergent
point BNC
( JP8)
【QBNC】
Distilled
waterBNC
(JP7)
DB.ZQ 【ZBNC】
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NOTE
The content in bracket is the code of plug.
Power on after connecting power board; test the voltage of each point by using multimeter. Check circuit and
components if the voltage is not in the range. Please see figure 63 for voltage range of test point of main
board.
U5 chip needs positive and negative voltage, positive +5V, negative 0.3V. 4.096V is the reference voltage
of transfer chip.
Drive board mainly achieve driven of each motor, it is divided into eight modules, one T module, six driven
modules (A to F) and one module for controlling pump and valve.
The main board sends instruction to drive board by P1; the driven part of reaction tray of T module is
controlled by CPU directly. The controlling signal of washing hand and other six modules is gained by 485
Bus. The T module only control the motor of washing hand which driven the up and down action, other six
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The work processing of drive board is as following: CPU send action instruction of one motor to drive board
by 485 Bus. Through DIP address selection, the corresponding module processor 89S52 of drive board
receiving instruction, the output signal strengthen reversely through 74240, then be sent to driven chip
THB6064, so that to driven motor.
Refer to figure 64 for control details and DIP jumper setup.
Figure 64 Driven module division and DIP address jumper
corresponding
Driven transferring Setting No. DIP address
Motor Remark
module connection line of motor jumper
of motor
Motor of
Turn 0 Nondial switch
reaction tray
T
Motor of
Lift 1 00001
washing hand
A not used
B not used
Motor of sample
Lift 2 00010
tray
C
Motor of reagent
Turn 7 00111
tray
Motor control up
and down action
Lift 8 01000
of aspirating
probe
F
Motor control
left and right
Turn 9 01001
action of
aspirating probe
Motor control up
and down action Lift 10 01010
D
of stirrer
Motor control Turn 11 01011
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Sample
Lift 12 01100
injection pump
E
Reagent
Turn 13 01101
injection pump
Using multimeter to test voltage of each test point of drive board. See figure 67. Check circuit and
components if the voltage is not in the range. Refer to figure 65 for voltage range.
Figure 65 Voltage of test point of drive board
Test point Voltage range(V)
J—Power 24±0.7
Dr.Bd1 12±0.7
PJ11(voltage of pin 1) 12±0.7
P5 5.1±0.2
PJ4(voltage of pin 3) 12±0.3
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Test output of P1 by multimeter after connecting transformer. If the voltage is not in range, adjust
potentionmeter until it’s in the range. See figure 67 for output voltage of power board. See figure 610 and
611 for line connecting and voltage testing.
Figure 67: Output voltage of power board
Test point Voltage range(V)
JP4 12±0.05
P1 5V1 5.2±0.1
P1 5V2 5.1±0.1
P1 +12V 12±0.2
P1 12V 12±0.2
P1 +5VA 5±0.1
P1 5VA 5±0.1
P1 +5VB 5±0.1
P1 5VB 5±0.1
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NOTE
The content in bracket is the code of plug.
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The transfer board receives instruction which from pump and valve controlling module by JP25 to control
pumps and valves. Voltage of solenoid and vacuum pump is 24V. See figure 612 and 613 for line
connecting.
Test the voltage of test point by using multimeter after connecting the lines of drive board. Check circuit and
components if the voltage is not in the range. See figure 68for voltage range of transfer board.
Figure 68: voltage of test point of transfer board
code Voltage range
24V 24±0.2V
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Figure 613 Diagram of line connecting of transfer board of pump and valve
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The function of motor transfer board is to receive drive signal from drive board, then separate into two parts,
includes LIFT and TURN. Each part could supply separate interface for connecting line of motor and
optocoupler.
There are six motor transfer boards. Only aspirating probe with JP2 and JP3 plug which function is
anticollision and liquid level sensing, the others have not.
See figure 69, 614, 615, 616 for detail line connecting method and voltage test point
NOTE
The content in bracket is the code of plug.
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The function of signal board is to convert optical signal into electrical signal. Then amplify and transfer to
main board.
There are ten silicon photocells on signal board, the function of these photocells are to receive optical signal
and convert into weak voltage signal, then amplify the signal by IC LTC6244 (U1_S3 to U1_S12, each
photocell corresponds one piece of LTC6244), then amplify signal again by OPA365 (U2), select by DG506
(U3), at last transfer to main board by PAMP1. See figure 617 for line connection.
UT1 is power supply chip which voltage is 5V; UT2 is power supply chip which voltage is 5V.
Test the voltage of test point by multimeter after power on. Check circuit and components if the voltage is not
in the range. See figure 610 for voltage range of signal board.
Figure 610 Voltage of test point of signal board
Code Voltage range
+5V 5±0.05V
5V 5±0.05V
+12V +12±0.2V
12V 12±0.2V
0.3V 0.3±0.05V
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NOTE
The content in bracket is the code of plug.
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The liquid level sensing board consists of liquid level sensing part and anticollision optocoupler.
Liquid level sensing adopts capacitive level induction principle. Probes link to U2 (MC3394) by JP2, and U2
converts capititance changes into voltage changes, then feedback to U1 (MC9S08SH8) for processing.
Alarming of reagent allowance could be achieved by U1.
The anticollision optocoupler has no output voltage under normal condition. The optocoupler will get
through if probe collision. Pin 4 will feedback collision signal to drive board, then control signal will be sent
from drive board. Probe will uplift to the top automatically to protect probe.
Test voltage of test point by multimeter after connecting liquid level sensing line. See figure 619 and 620.
Check circuit and component if the voltage is not in the range. See figure 611 for testing voltage range of
liquid level sensing board.
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Figure 619 Line connecting of liquid level sensing board and voltage test point (old version)
Figure 620 Line connecting of liquid level sensing board and voltage test point (old version)
NOTE
There is no obvious voltage test point on new version liquid level sensing board; its voltage is
changed by chip into 5V. If it is need to be test, please contact URIT.
The number of 1 to 4 is the label of jumper cap joint in figure 620; the jumper cap is used for programming
the procedure of liquid level sensing board and selecting sensitivity of liquid level sensing. See table 612 for
the function of jumper cap.
Table 612 Table of function of jumper cap group
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The light transfer board is a “transfer stop” of light power line, which separate light and power and convinent
for replacing light.
See figure 621 for line connecting of light transfer board
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Click Begin Run, input regedit, and then click confirm. Click my computer
HKEY_CURRENT_USERURITDIAGNOSE; it is the location of saving of parameter.
NOTE
System will prompt to drain water before test if there is water in cuvette.
In System parameter table, double click Directory to entry. That is the registry path of software which must
be accordance with actual path, otherwise will make mistake.
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Icon:
Function: Main control program
Icon:
Function: Test control program
Name: URITDIAGNOSE.mdb
Function: it is used for storaging all parameters, datas, include reagent parameter, test result, hospital setup,
QC and calibration datas, except mechanism parameter, instrument status and test curve.
Application: Copy this document before install new software, and replace the new document in new software,
so that the old data could be reserved.
Name: CheckData
Function: it is used for storaging test curve, a file is generated for one day,its name is the date when the file
is generated.
Application: The test curve data could be checked in different computer on URIT software after copying.
Name: Language
Function: it is used for storaging all text message, prompt information as Not found will appear if any loss or
damage.
Application: Modifying the text description according to requirement and the text will be displayed on
corresponding position.
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Different functions are configured for satisfying different requirements. A part of function is shielding since
different model with different hardware. The opening method and relative function are as follows:
Click D:\URIT Biochemical Analyzer, then open the Crazy.ini file by notepad or wordpad program.
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CHAPTER 8 MAINTENANCE
Please strictly according to the operating manual to operate and maintain instrument to guarantee the safety,
reliability and good working condition of instrument.
CAUTION
1) Please strictly according to the operating manual to operating instrument, otherwise damage
or personal injury will be occurred.
2) People who not trained or authorized by URIT, is not permit to repair the instrument.
3) Do not spill water, reagent and detergent on mechanical or electrical components.
Biological Hazard
Be sure to put on protective gloves, clothes, or even goggle if necessary.
8.1 Preparation
The following tools and detergent may be used in maintenance.
Tool
A set of hexagon wrench
Slotted point screwdriver (medium size)
Nozzle cleaner
Clean beaker
Tweezer
Clean gauze
Clean cotton swab
Brush (used for clean barrel)
Detergent
1) Alkaline detergent, concentrate detergent
2) Acid detergent
NOTE
1) The alkaline detergent is concentrate, dilute it with distilled water, the proportion is
1:9.
2) Please use the detergent supplied by URIT. If not, the test result is not reliable.
Others
Absolute alcohol
Clean beaker
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Do the following steps everyday to keep instrument under good working condition.
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If the connection is well, do the next step; if not, connect well as figure 83.
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Figure 86 Screw Cover of Detergent Barrel Figure 87 Take Down Cover of Detergent barrel
NOTE
Put the cover with liquid level sensor on the clean desk.
5) Dilute concentrate alkaline detergent with distilled water according to the proportion, then pour into
detergent barrel.
6) Put on cover with liquid level sensor and tighten.
7) Connecting BNC signal line.
Insert BNC signal to the joint and tighten.
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Biological Hazard
1) Be sure to put on protective gloves, clothes, or even goggles if necessary.
2) Dispose the waste solution according to local discharge standard, and consult reagent
supplier or distributor.
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Figure 813 Hold the BNC Line Figure 814 Unscrew BNC Line
Figure 816 Hold the BNC Line Figure 817 Insert BNC Line
Figure 818
8.2.4 Check Cleaning Position of Reagent and Sample Tray, Check Dilution Position
Check whether the detergent of No.60 position of reagent tray is enough or not, if not, sufficient it.
Check whether the detergent of No.59 position of reagent tray is enough or not, if not, sufficient it.
Check aspirating probes, stirrer and cuvettes. If there are any obvious stains, clean it according to weekly
maintenance.
Click Execute to perform probes and stirrer cleaning and cuvette rinsing after power on. See figure 819.
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Figure 819
Performing probe and stirrer cleaning, cuvette rinsing and add water before exit system. See figure 820.
Figure 820
Check the work condition of printer everyday and make sure the printing paper is enough.
Do the following maintenance every week to keep analyzer in best work state.
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CAUTION
Biological Hazard
1) Be sure to put on protective gloves, clothes, or even goggles if necessary.
2) Do not discard gauze which used for cleaning aspirating probes. Dispose it according to
relative regulation.
1) To make sure the test switch is off.
2) Gently uplift the aspirating probe rocker to the highest point and turn it to a position where convenient for
operating.
CAUTION
Do not touch the surface of probes and stirrer with tweezer when clean to avoid scratch. And
avoid apply too much pressure to make probe and stirrer bending.
3) Pick up a piece of gauze which with absolute alcohol to wipe surface of aspirating probe and stirrer from
up to down. Especial to wipe the needle tip until the surface is bright and clean.
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Figure 822
6) Click RESET, aspirating probes and stirrer will turn to washing pool and washed by distilled water.
Figure 823
Biological Hazard
Figure 824
3) Uplift the reagent tray bracket and pull out.
Figure 825
4) Using a piece of gauze with absolute alcohol to clean the surface of reagent and sample tray and
screws of holder, until there are no stains on the surface of reagent tray.
5) Using a piece of gauze with distilled water to clean the surface of reagent and sample tray and screws of
holder, until there are no alcohol trace on the surface.
6) Install the reagent and sample tray, tighten fixed screw then put on cover.
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Caution
Be careful to operate to avoid being scractched by needle tip.
Biological Hazard
1) Be sure to put on protective gloves, clothes, or even goggles if necessary.
2) Do not discard the cotton swab which used for clean washing pool. Discord it according
to the relative regulation.
Figure 826 clean washing pool Figure 826 clean washing pool
4) Switching on test switch.
5) Open system software and click Maintenance Probe and Stirrer Cleaning
Figure 827
Click Reset, aspirating probe and stirrer will turn to washing pool and cleaned by distilled water.
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Figure 829
Biological Hazard
Figure 830
CAUTION
DO NOT wipe reaction tray by gauze which with absolute alcohol or detergent since it will
cause corrosion of reaction tray. All losses thus incurred should borne by users.
CAUTION
The gauze should not with too much distilled water to prevent excessive water flow to cuvette.
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CAUTION
Be careful to operate to avoid being scractched by needle tip.
Biological Hazard
1) Be sure to put on protective gloves, clothes, or even glasses if necessary.
2) Do not discard gauze randomly, please dispose according to relative regulation.
CAUTION
Do not touches the surface of probe by tweezer to avoid scratch washing hand; and avoid
applies too much pressure to bend washing hand probe.
Biological Hazard
Be sure to put on protective gloves, clothes, or even glasses if necessary.
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Deep clean cuvette weekly to extend life and clean the deposited dirt on the inwall of cuvette.
1) Fill detergent barrel with detergent.
2) Click Maintenance Cuvette rinsing DeepClean.
Figure 832
Figure 833
3) Click Add Water
Figure 834
4) Click Cuv. Blank Reading
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Figure 835
5) Check the AD value in the list
Figure 837
All the AD value should be in the range of 40000 to 60000.
Please refer to the relative contents in the chapter of Check/Change Cuvette if value not in the range.
6) Click SAVE to save cuvette blank.
Figure 838
7) Click Drain water to pump all water out.
Figure 839
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Do the followings maintenance every month to keep analyzer in best work state.
NOTE
Do not scratch the cuvtte and stain the cuvette when take out the cuvette bracket.
NOTE
The cuvette bracket which under the washing hand is not easy to take out. Rotate the
reaction tray to a proper position then take out.
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NOTE
Do not scratch the light hole when clean the incubation slot.
Figure 847 Hold BNC Signal Line Figure 848 Screw off BNC Line
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NOTE
Do not scratch the liquid lever sensor, catheter and filter when using a brush to clean.
7) Using a clean gauze to clean the external and cover of distilled water barrel.
8) Insert distilled water pipe.
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Figure 851 Hold BNC Line Figure 852 Screw off BNC Line
Biological Hazard
Be sure to put on protective gloves, clothes, or even goggles if necessary. Discharge
waste solution according to local discharge standard and consult relative reagent
manufacturer and distributor.
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Figure 855 Hold BNC Line Figure 856 Screw off BNC Line
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NOTE
Do not scratch the liquid level sensor when use a brush to clean.
8) Using a clean gauze to clean the external and cover of waste solution barrel.
9) Insert waste solution barrel.
Figure 859 Hold BNC Line Figure 860 Insert BNC Line
Figure 865 Screw off Cover of Detergent Barrel Fiugre 866 Take out Cover with Liquid Level Sensor
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NOTE
Take out cover with liquid level sensor and put on clean desktop.
NOTE
Do not scratch the catheter and filter when using a brush to clean.
7) Clean liquid level sensor of detergent barrel by distilled water, using gauze to clean if necessary.
8) Using clean gauze to clean the external of detergent barrel.
9) Screw on cover with liquid level sensor of detergent barrel.
10) Connecting BNC signal line.
Insert the BNC signal line to the joint and tighten
Figu
re 867 Before Screwing Figure 868 After Screwing
Do not use alcohol or other corrosive detergent to clean drive shaft, otherwise will cause
stuck. Please use specified lubricant oil to maintain drive shaft.
Do not use alcohol or other corrosive detergent to clean drive shaft, otherwise will cause
stuck. Please use specified lubricant oil to maintain drive shaft.
Do not use alcohol to clean washing hand, using the detergent provided by URIT to
clean will be better.
It will appear scratch, stains on cuvette if used for a period. So it suggests replacing all cuvettes for every
three months to guarantee the accuracy of test result.
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Figure 870
2) Click Add water.
Figure 871
Click Cuv. Blank Reading
Figure 872
Figure 873
Check AD value of all cuvettes by clicking arrows of left and right.
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Figue 874
4) All AD values should be in the range of 40000 to 60000. If not, do the following step:
a) If the AD value exceeds 60000, adjust the potentionmeter according to 3.5.7.
b) If the AD value under the 40000, take out the cuvette which irradiate by light, observe the AD value, if it
is in the range, replace the cuvette; if there are no change or reduced, replacing light source. (refer to
8.6.3)
c) If the AD value of one row is too small, do the following steps:
To confirm the cuvette number which is problematic, the cuvette number corresponds with the number
on the left of list.
Screw off the fixed screws on the corresponding cuvette bracket.
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8.5.2 Clean Distilled Water Tank and Negative Pressure Tank for Draining Waste Solution
1) To make sure power switch is off.
2) Open rear baffle.
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Figure 880
This chapter mainly introduced the methods when aspirating probe is blocking, staining etc.
CAUTION
Be careful to operate to avoid scratch finger.
Biological Hazard
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NOTE
Do not lose the spring and retaining screw when take out the cover of probe.
5) One hand gently hold the joint of aspirating probe, the other hand pinches the joint of liquid level pipe
and take out.
NOTE
Pinch the joint of liquid level pipe with sand paper to increase friction force.
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Figure 884
Figure 885
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Figure 886
4) Loosen jackscrew and power joint of light, then take out lamp.
5) Connecting the lamp well and insert into lampsocket.
6) Turn on power switch and install the cover of reaction tray.
7) Run the software, click RESET or Roate to Cuv.1, when the original position of cuvette is confirm, click
Maintenance Cuvette signal
8) Take out cover of reaction tray again and adjusting the position of lamp, then observe the A/D signal.
9) When the A/D signal value is biggest, tighten the jackscrew to fix lamp.
10) At last install the cover and other components of reaction tray.
NOTE
If the reaction tray does not move to original position, the A/D value could not be read.
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Figure 888
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NOTE
Attention one to one correspondence when screw off the BNC line to avoid wrongly
insert.
If the instrument will not use for a long time, do the following steps before power off.
1) Click Maintenance Cuvette Rinsing.
Figure 889
Click WASH to rinse all cuvettes.
Figure 890
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Figure 892
Click Water Probe and Water Arm repeatly until there are no waste solution discharge from waste solution
tube.
Figure 893
5) Empty distilled water barrel, detergent barrel and waste solution tube.
Biological Hazard
Be sure to put on protective gloves, clothes, or even goggles if necessary.
According to the local discharge standard to dispose waste solution and consult reagent
manufacturer or distributor.
6) Turn off test switch, main power switch and pull out plug.
7) Close the up cover.
8) Put on a dust cover.
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9 TROUBLESHOOTING
This chapter lists the various malfunctions, along with probable causes and recommended remedies to
correct the problem quickly and easily. If the problem still exists after following the recommended remedy,
contact URIT for Technical support.
CAUTION
Handle malfunction with utmost care and confirm if it is necessary to cut off the power supply at the
first.
Biological Hazard
Put on protective gloves to avoid contacting chemical solution. If it spill to human body, wash it off
with water immediately.
To eliminate malfunction easily and correctly, users should read through the Operating Manual and be
familiar with the routine operation and maintenance of URIT8021A .In general, there are three steps to
handle with malfunction:
Ø Step 1: Confirming Malfunction
Users should not only confirm the malfunction, but also clearly know what the normal status should be when
the malfunction is eliminated.
Ø Step 2: Classifying Malfunction
Malfunction can be categorized into three types.
l Malfunction relating to hardware
l Malfunction relating to software
l Malfunction relating to operation and analyses.
If malfunction relates to the hardware or software, contact your local distributor or URIT. If malfunction
relates to the operation and analyses, refer to the troubleshooting table below for solution.
Ø Step 3: Eliminating Malfunction
The maintenance engineer authorized by URIT takes proper measures to correct the problem.
Users can also eliminate the malfunction under the directions of maintenance engineer.
Our Customer Service Office is available to help if the problem is beyond the scope of this manual or if you
need more technical assistance from URIT. Before calling, please identity the following information:
1) The instrument’s model;
2) The serial number of instrument;
3) The specific symptom and operating condition;
4) The data and report relating to the problem.
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The troubleshooting table below presents the various problems and malfunctions that may occur during
operation. If the problem can not be solved through the recommended methods, contact URIT please.
NOTE
For replacement parts of the instrument, refer to Appendix 1.
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Water or detergent
1) Flow path tube is leaky.
does not come out 1) Reconnect the tube or replace it.
2) Flow path tube is
through the probe 2) Unclog the tube.
clogged.
5 washing pool or 3) Replenish water or wash solution.
3) Water or wash solution is
stirrer washing 4) If the problem persists, contact your local
used up.
pool. distributor or URIT.
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10.2 Endpoint
In the endpoint measurement, the reaction reaches equilibrium after a period time, it can be considerate that
all analytes(substrates) have changed into products. It’s best that the absorbance is directly proportion to the
analytes’ concentration.
Endpoint method (single reagent) reaction graph see as figure 101:
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C =(A1 A0)´ K
C: The concentration of reactant
K: Calculation factor
A0: The average of absorbance from the first test point to the fifth test point before adding sample.
(R1 blank)
NOTE
When double reagents test use endpoint method, the result calculation is the same as the above
calculation formula that test point absorbance substracts R1 blank, then multiplied by K to get the
concentration.
C =(
[ A2 A0) ( A1 - A0 ) ´ k1 ] ´ K
C: The concentration of reactant
K: Calculation factor
A0: The average of absorbance from the first test point to the fifth test point before adding sample.
(R1 blank)
VR1 + VS
k1 =
VR1 + VS + VR 2
VR1: The volume of first reagent
VRS: The volume of sample
VR2: The volume of second reagent
Figure 103 TwoPoint Rate Method Graph (Single reagent, positive direction reaction)
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æ A - Aa ö
C = çç b ÷÷ ´ K
è tb - tb ø
C: The concentration of reactant
K: Calculation factor
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C = DAab / min´ K
C: The concentration of reactant
K: Calculation factor
NOTE
The double reagent calculation is the same as single reagent.
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Figure 105
Make experiment to obtain the absorbance of substrate exhaust and set the limit. The average value of the
last three points in positive reaction is greater than the absorbance value of substrate exhaust limit. If the
average value smaller than the absorbance value in negative reaction, it could judge as substrate exhaust.
The red box in the figure 106 is the substrate exhaust setup.
Figure 106
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The second method of substrate exhaust is using the slope ratio of reaction curve (absorbance change rate)
to judge.
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Figure 108
NOTE
The judgement method of double reagent is the same as the method in 10.4.2.
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8021A Circuit
Diagram.pdf
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