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Service Manual

(Engineering Edition)

URIT­8021A
Automatic Chemistry Analyzer
V1.0

URIT Medical Electronic Co., Ltd.


URIT‐8021A Service Manual

Catalogue
Copyright ....................................................................................................................................................................1
PREFACE......................................................................................................................................................................2
CHAPTER 1 INSTRUMENT INSTRUCTION .......................................................................................................................3
1.1 Brief Intrduction .......................................................................................................................................................... 3
1.2 Intended Use................................................................................................................................................................ 3
1.3 Structure ...................................................................................................................................................................... 3
1.4 Function of Main Part.................................................................................................................................................. 5
CHAPTER 2 PERFORMACE AND TEST FLOW...................................................................................................................6
2.1 Technical Parameter..................................................................................................................................................... 6
2.2 Operational Environment ............................................................................................................................................ 6
2.3 Test Process ................................................................................................................................................................. 7
2.3.1 Typical Test FlowChart...................................................................................................................................... 7
2.3.2 Test Process Instruction .................................................................................................................................... 8
CHAPTER 3 INSTALLATION AND DEBUGGING............................................................................................................... 12
3.2 Installation ................................................................................................................................................................. 14
3.2.1 Instrument Inspection .................................................................................................................................... 14
3.2.2 Installation ...................................................................................................................................................... 15
3.2.3 Environmental Requirements ......................................................................................................................... 15
3.2.4 Location Requirements................................................................................................................................... 16
3.2.5 Power Requirements ...................................................................................................................................... 16
3.2.6 Instrument Connection................................................................................................................................... 17
3.3 Installation of Aspirating probe and Stirrer ............................................................................................................... 18
3.3.1 Aspirating probe Installation........................................................................................................................... 18
3.3.2 Stirrer Installation ........................................................................................................................................... 21
3.4 Saftware Installation.................................................................................................................................................. 22
3.5 Instrument Adjustment .............................................................................................................................................. 26
3.5.1 Parameter Setup............................................................................................................................................. 26
3.5.2 Setup of Motor Speed..................................................................................................................................... 30
3.5.3 Parameter Setup of Liquid Path...................................................................................................................... 31
3.5.4 Setup of Initial Position................................................................................................................................... 33
3.5.5 Adjusting Position Parameter to Washing Pool .............................................................................................. 38
3.5.6 Adjusting Position of Reagent /Sample Tray ................................................................................................... 40
3.5.7 Debugging of Optical Path .............................................................................................................................. 41
CHAPTER 4 MECHANICAL UNIT MODULE .................................................................................................................... 44
4.1 Shell and Structure of the Machine ........................................................................................................................... 44
4.1.1 Shell ................................................................................................................................................................ 44
4.1.2 Disassembly and Instructure .......................................................................................................................... 45
4.2 Aspirating Unit .......................................................................................................................................................... 50
4.2.1 Function.......................................................................................................................................................... 50
4.2.2 Structure ......................................................................................................................................................... 50
4.2.3 Aspirating probe Installation........................................................................................................................... 52
4.3 Stirrer Unit................................................................................................................................................................. 52
4.3.1 Function.......................................................................................................................................................... 52
4.3.2 Structure ......................................................................................................................................................... 53
4.3.3 Stirrer Installation ........................................................................................................................................... 54
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4.4 Reagent /Sample Tray Unit........................................................................................................................................ 54


4.4.1 Function.......................................................................................................................................................... 54
4.4.2 Instructure ...................................................................................................................................................... 55
4.4.3 Install and Disassemble Reagent/Sample Tray................................................................................................ 55
4.4.4 Install and Disassemble Refrigeration module ............................................................................................... 59
4.5 Reaction Unit............................................................................................................................................................. 60
4.5.1 Function.......................................................................................................................................................... 60
4.5.2 Structure ......................................................................................................................................................... 60
4.5.3 Install and Disassemble Reaction Tray ............................................................................................................ 61
4.6 Washing Hand Unit.................................................................................................................................................... 62
4.6.1 Function.......................................................................................................................................................... 62
4.6.2 Structure ......................................................................................................................................................... 62
4.6.3 Installation and Disassembly .......................................................................................................................... 62
4.7 Photoelectric Detection Unit ..................................................................................................................................... 63
4.7.1 Function.......................................................................................................................................................... 63
4.7.2 Structure ......................................................................................................................................................... 63
4.8 Install and Disassemble Injection Pump.................................................................................................................... 66
CHAPTER 5 LIQUID PATH ............................................................................................................................................ 67
5.1 Function..................................................................................................................................................................... 67
5.2 Liquid Path Diamgram .............................................................................................................................................. 69
5.3 List of Valve and Pump....................................................................................................................................... 69
CHAPTER 6 ELECTRONIC CIRCUIT OF HARDWARE ........................................................................................................71
6.1 Summary ................................................................................................................................................................... 71
6.2 List of Circuit Board.................................................................................................................................................. 71
6.3 Position of Circuit Board........................................................................................................................................... 71
6.4 Principle of Circuit Board.......................................................................................................................................... 73
6.5 Function of Circuit Board.......................................................................................................................................... 73
6.5.1 Main board ..................................................................................................................................................... 73
6.5.2 Main Board ..................................................................................................................................................... 75
6.5.3 Terminal Board................................................................................................................................................ 78
6.5.4 Power Board ................................................................................................................................................... 79
6.5.5 Transfer Board of Pump and Valve.................................................................................................................. 81
6.5.6 Motor Transfer Board ..................................................................................................................................... 83
6.5.7 Signal Amplify Board....................................................................................................................................... 85
6.5.8 Liquid Level Sensing Board ............................................................................................................................. 87
6.5.8 Light Transfer Board........................................................................................................................................ 89
CHAPTER 7 SOFTEWARE PARAMETER .........................................................................................................................90
7.1 General Parameter in Registry Table ......................................................................................................................... 90
7.1.1 Way to Entrance.............................................................................................................................................. 90
7.1.2 Sign of Simulation Mode................................................................................................................................. 90
7.1.3 Sign of Having Water in Cuvette ..................................................................................................................... 90
7.1.4 Path of Software Registration ......................................................................................................................... 90
7.2 Common Files of Software........................................................................................................................................ 91
7.2.1 Main Procedure .............................................................................................................................................. 91
7.2.2 Test Program ................................................................................................................................................... 91
7.2.3 Data Base ........................................................................................................................................................ 91
7.2.4 Test Data Folder .............................................................................................................................................. 91

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7.2.5 Language Package Folder................................................................................................................................ 91


7.3 Method to Open Common Function.......................................................................................................................... 92
CHAPTER 8 MAINTENANCE ........................................................................................................................................ 93
8.1 Preparation................................................................................................................................................................. 93
8.2 Daily Maintenance..................................................................................................................................................... 94
8.2.1 Check Distilled Water...................................................................................................................................... 94
8.2.2 Check Detergent Barrel................................................................................................................................... 95
8.2.3 Clean Waste Solution Barrel ........................................................................................................................... 97
8.2.4 Check Cleaning Position of Reagent and Sample Tray, Check Dilution Position ............................................. 99
8.2.5 Check/Clean Aspirating Probes, Stirrer and Cuvettes..................................................................................... 99
8.2.6 Check Printer and Printing Paper.................................................................................................................. 100
8.3 Weekly Mintenance......................................................................................................................................... 100
8.3.1 Aspirating Probes and Stirrer........................................................................................................................ 101
8.3.2 Clean Reagent Tray and Sample Tray ............................................................................................................ 102
8.3.3 Clean Washing Pool ...................................................................................................................................... 103
8.3.4 Clean Reaction Tray....................................................................................................................................... 105
8.3.5 Clean Washing Hand..................................................................................................................................... 106
8.3.6 Clean Panel ................................................................................................................................................... 106
8.3.7 Deep Clean Cuvette ...................................................................................................................................... 107
8.3.8 Check AD Value and Save Cuvette Blank ...................................................................................................... 107
8.4 Monthly Maintenance.............................................................................................................................................. 109
8.4.1 Clean Incubation Bath................................................................................................................................... 109
8.4.2 Clean Distlled Water Barrel........................................................................................................................... 111
8.4.3 Clean Waste Solution Barrel ......................................................................................................................... 113
8.4.4 Clean Detergent Barrel ................................................................................................................................. 116
8.4.5 Clean Drive Shaft of Aspirating Probe........................................................................................................... 118
8.4.6 Clean Drive Shaft of Stirrer ........................................................................................................................... 118
8.4.7 Check Washing Hand .................................................................................................................................... 118
8.5 Maintenance for Every Three Months............................................................................................................. 118
8.5.1 Change Cuvette and Cuvette Bracket ........................................................................................................... 118
8.6 Non­Periodical Maintenance ................................................................................................................................... 122
8.6.1 Clear/Replace Aspirating Probe .................................................................................................................... 122
8.6.2 Replacing Stirrer ........................................................................................................................................... 124
8.6.3 Replacing Lamp............................................................................................................................................. 125
8.6.4 Solenoid Valve Rinse..................................................................................................................................... 127
8.6.5 Check/Replacing BNC Line............................................................................................................................ 127
8.6.6 Replacing Waste Solution Tube..................................................................................................................... 128
8.7 Long­time Disuse Maintenance............................................................................................................................... 128
9 TROUBLESHOOTING .............................................................................................................................................. 130
9.1 Troubleshooting Guide ............................................................................................................................................ 130
9.2 Obtaining Technical Help ........................................................................................................................................ 130
9.3 Troubleshooting Method.......................................................................................................................................... 131
CHAPTER 10 Measurement Method ......................................................................................................................... 135
10.1 Method of Measurement........................................................................................................................................ 135
10.2 Endpoint ................................................................................................................................................................ 135
10.3 Two­Point End Method (Double Reagent Endpoint)............................................................................................. 136
10.3 Two­Point Rate Method (Fixed­Time) .................................................................................................................. 137

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10.4 Rate Method (Kinetic method) .............................................................................................................................. 138


10.5 Substrate Exhaust .................................................................................................................................................. 139
10.5.1 Substrate Exhaust Method 1(Substrate exhaust Limit) .............................................................................. 140
10.5.2 Substrate Exhaust Method 2(Slope Ratio).................................................................................................. 141
Appendix A URIT‐8021A Circuit Schematic Diagram .............................................................................................. 143

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URIT‐8021A Service Manual

Copyright
Copyright: URIT Medical Electronic Co., Ltd.

URIT Medical Electronic Co., Ltd. owns the intellectural property rights to this service manual. No part of this
manual may be reproduced, stored or transmitted in any form, or by any means without the express written
permission of URIT.

Customer Service
URIT Medical Electronic Co., Ltd.
ADD: No.3 Fuhe Alley, Zhonghua Road, Guilin, Guangxi 541001, PR China
Customer Service hotline: 86 773 2288566 86 773 2825742
400­ Service hotline: 400 727 2288

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URIT‐8021A Service Manual

PREFACE
This document is the service manual for URIT­8021A discrete random access chemistry analyzer. It
describes the structure, operation, maintenance and troubleshooting concerning the instrument in details.
Users should read carefully the manual and get special training before operating to guarantee instrument
precision, normal operation and personal safety.

Sign Illustration
Meaning of the signs used in the URIT­8021A is as following.
Caution. Refer to the
Caution. Electric shock
accompanying document

Caution. Hot surface Biohazard

Ground Power on

In vitro diagnostic
Power off
medical device

Environmental protection Keep away from heat and


lifetime radioactive source

Serial number Manufacturer

Recovery May cause personal injury

Refer to the operating manual

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URIT‐8021A Service Manual

CHAPTER 1 INSTRUMENT INSTRUCTION


1.1 Brief Intrduction

URIT­8021A is clinical chemistry instrument with the characteristics of open, full­automatic, discrete, STAT
priority and computer­controlled. URIT­8021A is intended for use in conjunction with reagents to measure
quantitatively certain chemical items in serum, urine and cerebrospinal fluid. Please read the operating
manual carefully before using since it is a high sophisticated instrument.

1) Work Unit consists of optical unit, mechanical operation unit, liquid path control unit, hardware circuit
unit and operating unit.

2) Mechanical operation unit consists of aspirating system, stirring system and eight­phase washing
system.aspirating system includes sample tray, reagent tray, aspirating mechanism, aspirating probe
(the inner core of syringe is made of ceramic which aspirating accurately and maintenance­free ) and
sample probe wash station.The sample tray has 45 positions, which contains 35 rountine positions, 5
standard positions, 3 QC positions and 2 STAT positions. Tube or sample cup could be placed on
routine position. The reagent tray could place the reagent bottle in different specifications. The optical
unit is a whole sealing, statics, array and rear spectrophotometry optical system, which includes 120
cuvettes, high­resolution filter and halogen light.

3) The operating unit is a external computer (CPU 2.0 GHz or above, 16XDVD drives, memory: 1GB or
above) , the application software should be setup under the Windows XP operation system.

4) Liquid path control unit consist of vacuum pump, solenoid valve, syringe, rinse system and tube system.

5) The instrument is easy to operate. The layout of the screen menu is reasonable, name is simple. Such
as testing parameter setup, patient’s information input, quality­control, reagent, data query, standard,
running test and hardware parameter. After setting, put the sample and reagent to the instrument and
begin to analyze. Print out the result by the external printer at last.

1.2 Intended Use

The instrument is only for professional, in vitro use in hospitals, clinics and laboratories.
CAUTION
Please contact the reagent manufacturer or distributor if some samples may not applicable to
analytical test according to the test parameter and reagent.

1.3 Structure

The instrument structure mainly consists of optical system, reaction system, rinse system, sample system,
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URIT‐8021A Service Manual

reagent system, liquid path system and software system.

The instrument is mainly composed of analyzer, computer and printer. Printer is optional accessory.

The analyzer mianly consists of reagent tray, sample tray, reaction tray, aspirating system, stirring system,
optical system, rinse system, liquid path system and hardware circuit.

User could select cabinet as needed.

Figure 1.3.1 Overview of Analyzer

Figure1.3.2 Top View of Analyzer

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1.4 Function of Main Part

Main workflow:
1) Mechanism unit startup the initialization progress
2) The eight­step wash mechanism washing the cuvettes automatically (No this step, if you do not select
the clean before test option).
3) A washed and clean cuvette rotated to reagent aspirating position.
4) The reagent tray convey first reagent to aspirating position. The aspirating probe (aspirating mechanism)
turn to the aspirating position and move down to absorb first reagent, then deliver it to cuvette.
5) First reagent in the cuvette preheated several cycles and then moved to sample aspirating position.
6) The sample tray conveys the sample to aspirating position. The aspirating probe (aspirating mechanism)
turn to the aspirating position and move down to absorb sample, then deliver it to the cuvette.

7) Cuvette which with reagent and sample solution moved to stirring position to stir.

8) for the item which use double reagent for testing:

a) Constant speed mode: After fixed cycle, cuvette rotated to the relative position, the reagent tray
convey second reagent to aspirating position. The aspirating probe (aspirating mechanism) turn to
the aspirating position and move down to absorb second reagent, then deliver it to the cuvette.

b) Mixed mode: After arrive the incubate time of first reagent, cuvette rotated to the relative position,
the reagent tray convey second reagent to aspirating position. The aspirating probe (aspirating
mechanism) turn to the aspirating position and move down to absorb second reagent, then deliver it
to the cuvette.

9) Cuvette moved to stirring position to stir after the addiction of second reagent.

10) During each cycle, each of the cuvettes transmitted to the photometric position (the light source position)
for automatic signal collecting and absorbance calculation.

11) Eight­step washing mechanism washes the cuvettes automatically after finishing the test.

Table 1.4.1: Function of main part


Unit Function
Aspirating probe unit Absorb or inject the sample and reagent for all the biochemical items.
Sample tray unit 45 sample positions for all samples, STAT samples, controls and calibrators.
Reagent tray unit 60 reagent positions for all reagents, detergent and diluents.
Reaction tray unit 120 cuvettes to provide reaction and detection place.
Stirring unit Stirring and mixing the liquid in cuvette filled with sample or second reagent.
Optical test system It’s contains ten wavelengths and test the absorbance of liquid in the cuvette.
Automaic wash unit Provide eight­step auto wash cuvette function.

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CHAPTER 2 PERFORMACE AND TEST FLOW


2.1 Technical Parameter

1) System: full­automatic, discrete/optional, STAT priority function


2) Testing speed: 280 tests/h (pure biochemistry)
3) Item storage: more than 1200
4) Data storage: Infinite storage according to the storage space of computer.
5) Method method: Endpoint, Rate(kinetic method), 2­point endpoint, 2­point rate (2­point kinetic), double
wavelength, blank(reagent blank, sample blank), immunoturbidimetry etc.
6) 120 cuvettes, the cuvettes colorimetric directly.
7) Calibration method: linear and non­linear calibration, multi­point linear.
8) Sample tray: The primitive tube which specification is 5mL, 7mL and 10mL or specified sample cup.
9) Reagent tray: 60 reagent positions with refrigeration function, which could place reagent bottle in
various specification.
10) Sample Volume: 2 to 100 , variable in 0.1
11) Min. reaction volume: 150
12) Optical system: whole sealing, static, array and rear spectrophotometry optical system, 10 wavelengths
for selecting.The wavelength accuracy is 2nm.
13) Light source: halogen lamp, 12V/20W.
14) Washing system: unique washing system for aspirating probe and stirrer. Eight­phase washing system.

2.2 Operational Environment

1) Water consumption: 3L/H distilled water


2) Temperature control: 37 0.1 degree centigrade
3) Power: AC220, 50/60Hz, 500VA
4) Fuse: T8AL 250V
5) Relative Humidity: not exceed 85 percent
6) Environmental temperature: 10 to 35 degree centigrade
7) Instrument Dimension: 102cm 83cm 112cm (L W H)

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2.3 Test Process

2.3.1 Typical Test FlowChart


NOTE
Two kinds of test modes in URIT­8021A are mixed mode and constant speed mode, each
period time of them are respectively 18s and 24s. Choose the mode according to need.
Instrument could be chosen to automatic wash cuvette before test or directly test.

2.3.1.1 Mixed Mode

Figure 2­1 Mixed Mode Flowchart

Instruction of the mixed mode flowchart is as following:


1­Start 6­Stir sample 11­Finish the test
2­Initialization 7­Double reagents test 12­Washing cuvettes
3­Washing cuvettes automatically 8­Single reagent test 13­Stop automatically
4­Add first reagent 9­ Add second reagent
5­Add sample 10­Stir reagent

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2.3.1.2 Constant Speed Mode

Figure 2­2 Constant Speed Mode Flowchart

The meaning of the numbers in the figure 2­2 is the same as the figure 2­1, please refer to 2.3.1.1

2.3.2 Test Process Instruction

2.3.2.1 Sample Aspirating Procedure

The procedure of sample aspirating in each period is as follows:


1) The aspirating probe uplift from washing pool.
2) Turn to the above of sample tray.
3) Down to the sample cup (or tube) and absorb sample.
4) Uplift and turn to the above of reaction tray.
5) Down to the cuvette and inject the sample.
6) Uplift from cuvette and turn to the above of washing pool.
7) Down to washing pool for bubble type washing.
(procedure of sample aspirating in next period)

2.3.2.2 Reagent Aspirating Procedure

The procedure of reagent aspirating in each period is as follow:


1) The aspirating probe uplift from washing pool.
2) Turn to the above of reagent tray.
3) Down to the reagent bottle and absorb first reagent.
4) Uplift and turn to the above of reaction tray.

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5) Down to the cuvette and inject the reagent.


6) Uplift from cuvette and turn to the above of washing pool.
7) Down to washing pool for bubble type washing.
(procedure of reagent aspirating in next period)
The procedure of second reagent aspirating is the same as first reagent.

2.3.2.3 Stirrer Work Procedure

The stirrer work procedure in each period is as follow:


1) The stirrer uplift from washing pool.
2) Turn to the above of reaction tray.
3) Down to the cuvette.
4) Stirring the mixed solution in cuvette.
5) Uplift from cuvette and turn to the above of washing pool.
6) Down to washing pool for bubble type washing.
(procedure of stirring in next period)

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2.3.2.4 Reaction Tray Work Procedure

Figure 2­3 Reaction Layouts

1­No.35, Square Wiper 6­No.81 light spot


2­Adjusting left/right for optocoupler 7­No.44, the first nozzle initial position
3­Adjusting front/rear for optocoupler 8­Optocoupler bracket
4­No.1, aspirating probe initial position 9­Light source
5­No.110, stirrer initial position

2.3.2.4.1 Mixed Mode Procedure

The sequential procedure of reaction tray in mixed mode:


Reaction tray provides 120 positions for cuvettes, and rotates twice in a clockwise direction and stops two
times in a period. After the second stop, the reaction tray has completed the following procedures:
1) First reagent:
a) First stop: Aspirating probe absorbs sample from sample cup and then injects to cuvette.
b) Second stop: Aspirating probe absorbs first reagent from reagent bottle and injects to cuvette. Then

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the stirrer turns to cuvette to stir the mixed solution.


2) Second reagent:
a) First stop: Aspirating probe absorbs second reagent from another reagent bottle and injects to
cuvette.
b) Second stop: Then the stirrer turns to cuvette to stir the mixed solution.

2.3.2.4.2 Constant Speed Mode Procedure

The sequential procedure of reaction tray in constant speed mode:


Reaction tray provides 120 positions for cuvettes, and rotates three times in a clockwise direction and stops
three times in a period. After the third stop, the reaction tray has completed the following procedures:
1) First stop: Aspirating probe absorbs second reagent from reagent bottle and injects to cuvette.
2) Second stop: Aspirating probe absorbs sample from sample cup and then injects to cuvette. Then the
stirrer turns to cuvette to stir the mixed solution.
3) Third stop: Aspirating probe absorbs first reagent from another reagent bottle and injects to cuvette,
then stirrer turns to cuvette to stir the mixed solution again.

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CHAPTER 3 INSTALLATION AND DEBUGGING

3.1 Safty Precautions

Please comply with the following rules for safety and active use.

Preventing Breakage and Flammability


Please observe the following precaution for prevention breakage.
CAUTION
1) Installation must be complied with the installed environment in the manual
2) If relocation is necessary, contact your local distributor or URIT firstly.

Preventing Injury
Please observe the following precautions for preventing injury.
CAUTION
1) While the instrument is in motion, DO NOT touch the moving parts, such as sample probe,
reagent probe and stirrer, etc. Otherwise, you may be injured.
2) Before lamp replacement, turn off the power switch and wait until the lamp is cooled down.
Otherwise, you may receive burns.

Chemical and Biological Safety


Please comply with the following matters for chemical and biological prevention.
Biological Hazard
Inappropriate handling of sample may lead to biohazardous infection. Do not touch the
sample, mixed solution and waste directly with your hands. Be sure to wear gloves and lab
coat, if necessary, goggles. In case your skin contacts the sample, please follow standard
laboratory safty procedure and consult a doctor.

CAUTION
Some reagents are strong acid or alkaline. Please use them carefully avoiding direct contact. If
the reagent sticks to the human body, immediately wash it off with water and soap. If the
reagent splashes into eyes accidentally, wash it off with water and consult an oculist.

Disposing Wastes
Please comply with the following matters when treat the wastes in order to avoid personal injury and protect
environment.

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Biological Hazard

Some substances contained in reagent, control, calibrators and waste are subject to regulations
of contamination and disposal. Dispose of the waste in accordance with your local or national
rule for biohazard waste disposal.

Operational Environment
CAUTION
1) Please install the instrument according to the specified installed instruction in the manual.
Otherwise, the results may not reliable even may cause system damage.
2) Please contact URIT if system state changed is necessary.

Systematic Usage
CAUTION
1) The operator must receive training before operating the instrument. Please follow the
instruction of the manual to operate. Improper operation may cause personal injury, system
damage and improper result.
2) Please make a calibration and quality­control test when use the system for the first time to
ensure it can be used normally.
3) A quality­control test must be done when use the system. Otherwise, the reliability of the
result could not be guaranteed.
4) The communication interface of analytic part is set to connect with the communication
interface of operation part. Please use the cables of URIT for connecting.
5) The computer should be for the instrument exclusive use. DO NOT run any other software
with the computer while it is connected with the instrument. Inappropriate manner may result
computer virus infection.

Caution on Electromagnetic Wave Interference


CAUTION
Keep the instrument away from strong noise source and electromagnetic wave. Turn off mobile
phones and transmitter­receiver when operating the instrument as the electromagnetic wave
may cause an adverse effect on instrument.

Other Cautions
CAUTION
1) DO NOT touch the keyboard, indicator and mouse when your hands is wet, also includes the
chemistry.
2) Check samples for contamination (dust, or fibrinogen) and air bubble before analyses.
3) To make periodic maintenance, test and replacing according to the manual for getting the
exact result.
4) For replacements of major parts, such as light source lamp, aspirating probe, reaction cuvette
etc, please contact URIT.
5) For settings of sample volume, reagent volume, wavelength, standard values etc, please
refer to the instruction in reagent kit as well as this operating manual. Take note of checking
the quality of distilled water and detergent, calibration results, control results, and sample
results. Make sure there is no air bubble in the liquid paths.

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3.2 Installation

WARNING
The URIT­8021A analyzer should be installed only by technicians of or authorized by URIT.
Only the technicians of or authorized by URIT can perform the installation of URIT­8021A, users shall make
preparation for satisfying the installation requirements in accordance with this manual before installation. If
relocation is necessary, please contact your local distributor or URIT.

3.2.1 Instrument Inspection

Please check the carton according to the following procedures:


1) Loosen 8 buckles in the bottom of wooden box and uplift the box then take out cabinet. Open the door of
cabinet and take out distilled water barrel, waste solution barrel and wash solution barrel. See figure 3­1

Figure 3­1

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2) Take out the analyzer and put on the cabinet. See figure 3­2

Figure 3­2
3) Open the package of liquid path and take out.
4) If any loss or damage exists, contact the distributor or manufacturer immediately.

3.2.2 Installation

The instrument is high sophisticated thus proper installation is very important to its performance. User
should guarantee the environment and electrical condition are comply with the recommended conditions.
Provide a distance of 50cm at least for each side for operating and maintaining.

3.2.3 Environmental Requirements

1) Keep away from direct sunlight.


2) Only for indoor installation.
3) Dust free.
4) Installed on horizontal ground (Gradient less than 1/200).
5) Ground load weight: 250kg
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6) Room temperature: 10 to 35 degree centigrade.


7) Relative Humility: not exceed 85 percent (No condensation).
8) 86kPa~106kPa; Atmosphere Pressure: 86 to 106 KPa.
9) The reliability of the data could not be guaranteed if the fluctuation of temperature and humility
exceeded the certain range.
10) Good ventilation and do not face air conditioner.
11) No obvious vibration.
12) Keep away from electromagnetic field and electricity interruption, such as cellphone, radio transceiver,
Brush­type engine and the electrical equipment frequently turn on and off.
13) The instrument should be near to the power.

3.2.4 Location Requirements

The instrument installation layout is below. Surrounding distance is the recommended maintenance space.

Measure: cm
Instrument Dimension: 89 73 112cm (L W H)
Dimension of Operating Board (Only for reference): 70 50 80cm (L W H)

3.2.5 Power Requirements

The following power must be prepared; switchboard should be located within 10m.
1) Power
Voltage: AC 230V, 50/60Hz
2) Grounding
Using three­pin power plug
3) Plug board
A 15A output plug board with more than three 5A sockets. Heavy­duty devices should not share the plug
board with the instrument, such as refrigerator, air conditioner etc.
4) 3 core power cable cat is using; the type of wire and plug is depended on voltage.
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CAUTION
Make sure the instrument is grounded properly. Poor grounding may cause bad effects on test
result and even damage to the instrument.

3.2.6 Instrument Connection

Figure 3­3 Instrument Connection


The instrument connection detail steps are as follow:
1) Take out the power line from accessory box, one end inserted into the power interface of instrument, the
other end connected to the power.
2) Using the COM port communication line which provided by URIT for connection. One end connected to
the RS232 Port of instrument, the other end connected to the COM port of computer. And please tighten
by the screws.
3) Tighten communication interfaces by screws to avoid falling off and inaccurate communication.
4) Connecting the BNC interfaces on the rear of instrument to the corresponding level sensing interface on
the barrel.
Tube connection of instrument and liquid path box:

To connect the liquid path tubes according to the marks on tubes and the silk­printing on the rear of the
instrument.

CAUTION

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URIT‐8021A Service Manual

If you drain the waste solution into sewer directly without waste barrel, please short connecting
the interface of waste solution BNC to prevent the alarm that waste solution is full. Otherwise,
the liquid level sensing buzzer will alarm.

3.3 Installation of Aspirating probe and Stirrer

In order to prevent damage during transportation, aspirating probe and stirrer are disassembled and packed
individually, then reinstalled and debugged after arrive the destination.

3.3.1 Aspirating probe Installation

1) Uplift the arm of the aspirating probe to the highest position and then rotate it to the above of reagent
/sample tray.

Figure 3­4 Sampling Arm


2) Gently pinch the sampling arm cover and remove the cover from aspirating arm.

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URIT‐8021A Service Manual

Figure 3­5 Take down Sampling Arm Cover


(3)Take out the aspirating probe from the accessory box. See as the figure 3­6, 3­7.

Figure 3­6

Figure 3­7
3) Remove the retaining screw, insert the aspirating probe downwards into the positioning hole and tighten
the retaining screw, then connect the liquid level sensing connector on the circuit board. See as the
figure 3­8.

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URIT‐8021A Service Manual

Figure 3­8
NOTE
1) Keep the spring carefully, when remove the retaining screw.
2) Uplift sampling arm to the highest point before installing aspirating probe. Make sure the
anti­collision plate installed between anti­collision optocouple and spring pressed on
location plate.

Figure 3­9

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3.3.2 Stirrer Installation

1) Gently pinch the stirrer cover and remove it.

Figure 3­10

NOTE
Keep the flat washers and spring washers carefully to prevent fall into the instrument, when
remove the restaining screws of stirrer.

2) Take out the stirrer from the accessory box.

Figure 3­11
3) Remove the retaining screws; insert it downwards into the positioning hole, then tighen the restaining
screw.

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URIT‐8021A Service Manual

Figure 3­12
NOTE
The positioning hole for adjusting the front and rear position of stirrer. Two screws on the
adjusting cover do not need to fix tigh before adjusting the initial position.

3.4 Saftware Installation

1) Click a message box is displayed as figure 3­13.

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URIT‐8021A Service Manual

Figure 3­13
2) Click Next, the dialog box to choose the installation location is shown as figure 3­14.

Figure 3­14
3) Click Next, the message box is displayed as figure 3­15.

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URIT‐8021A Service Manual

Figure 3­15

4) Click Next, the message box is displayed as figure 3­16 to choose whether create a desktop icon or not.

Figure 3­16
5) Click Next, the message box is shown as figure 3­17. Then click Install botton to perform the installation
process.

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URIT‐8021A Service Manual

Figure 3­17
6) The message box will be shown as the figure 3­18 after complete the installation, then click Finish to
exit.

Figure 3­18

7) Double­click to run the software.

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URIT‐8021A Service Manual

8) Click Help Software Registration to enter registration interface shown


as figure 3­19

Figure 3­19
Send the User ID to After­Sale Service to obtain the License Number, and then input the number in blank

box. Click , a prompt box will popup, shown as figure 3­20.

Figure 3­20
Click OK, then close the software and restart according to prompt, the installation is completed.

3.5 Instrument Adjustment

This section mainly introduces the instrument adjustment method. Please make sure the correct connection
of piping and wiring.

3.5.1 Parameter Setup

1) Double­click , then select Server for adjusting and input the password 3112772 into the main
interface.
2) Turn on the red power switch, then green testing switch. And check that if abnormal for instrument.

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3) Click Maintenance Instrument Parameter , then input the password


3112749 to enter instrument parameter interface , see figure 3­21.

Figure 3­21
4) The instrument has been adjusted and the parameters restoraged in the main board before leaving
factory. Click

to get parameters from main board when adjust.


NOTE
1) Please check the parameters according to 3.5.2­­­3.5.7 in this manual after parameter get
for the position of component changed by transportting.
2) The Parameter Table in accessories box in each instrument is used in adjusting.

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URIT‐8021A Service Manual

CAUTION

1) DO NOT click before all instrument parameters being set properly.


2) On adjusting interface, the corresponding mechanism begins to action only after being

selected and clicking .

3) Please click if you are not sure the mechanism’s direction of


movement.

Meaning of each parameter


NOTE
1) The boxes with red words or grey color are not available.
2) Since the sample and reagent share a aspirating probe, please adjust the postions of
aspirating probe to cuvette, washing pool, sample, reagent to complete the adjustment of
sample probe and reagent probe.

l Sample tray and reagent tray initial position:


1) 31­R: Pos. 31 of the reagent tray rotates to the reagent aspirating position.
2) 1­R: Pos.1 of the reagent tray rotates to the reagent aspirating position.
3) 1­S: Pos.1 of the sample tray rotates to the sample aspirating position.

l Setup of Aspirating probe Movement:


1) To cuv.: The aspirating probe will move over to the cuvette
2) Vertical: The aspirating probe will move down into the cuvette.
3) To cleaning: The aspirating probe will move over to the above position of washing pool.
4) Vertical: The aspirating probe will move down into the washing pool.
5) R.position to S.position: The aspirating probe will move over sample aspirating position.
6) To S.position vertical: The aspirating probe will move down to the cuvette.
7) To 31­R: The aspirating probe will move over to the reagent aspirating position of the outer ring on
reagent tray.
8) To 1­R: The aspirating probe will move over to the reagent aspirating position of the inner ring on
reagent tray.
9) To R.bottle vertical: The aspirating probe will move down into the reagent bottle.

l Cuvette :
Cuv.1: Cuv.1 will move over to the initial position of reaction tray.

l Reset:
Reset: It does affect any operation if not perform resetting after turn on the power.
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l Setup of Stirring Arm Movement


1) R.stirrer to cuv.: The stirrer will move to the cuvette.
2) Vertical:The stirrer will move down into the cuvette.
3) To cleaning: The stirrer will move to the above position of washing pool
4) Vertical: The stirrer will move down to the washing pool.

l Fine Adjustment:
Perform fine adjustment for parameters of selected item.
There are four commands up, down, right and left to adjust the parameter of selected item.

l Cuvette rinsing arm


Arm to Cuv. Vertical: The cuvette rinsing arm moves down into the cuvettes.

l Test:
It is not available if the button of Test display grey color, click the Adjust button to operate it.

CAUTION
The buttons will come into action immediately after clicking. Make sure that all the mechanism is
in Arm raised state to prevent probe collision.
1) Reaction cuvette: The corresponding cuvette will rotate to the reagent aspirating position.
2) S. position: The corresponding sample cup will rotate to the sample aspirating position.
3) R. position: The corresponding reagent bottle will rotate to the reagent aspirating position.
4) Reset: The corresponding sample or reagent injection pump will rotate to the initial position.
5) Sam. In: The sample injection pump will aspirate quantitative volume of sample, measured by ul.
6) Reg. In: The reagent injection pump will aspirate quantitative volume of reagent , measured by ul.
7) Out: The sample or reagent injection pump will dispense the volume aspirated in the previous step.
8) S.probe fill water: the liquid path is open for washing the inner wall of sample probe, take times as unit.
9) Wash arm fill water: the liquid path is open for washing the inner wall of reagent probe, take times as
unit.
10) Running gear: Running in the moving component of reaction tray.
11) Stop: Stop running gear.

l The function of other buttons:


1) Speed Set: Enter speed setup interface
2) Fluid Set: Enter liquid path setup interface
3) Switch On: Turn on the corresponding valves and pumps
4) Switch Off: Turn off the corresponding valves and pumps
5) Stop All Motor: Stop all motions of electric motor
6) Sample­detection: Test the communication between instrument and computer.

l Functional Buttons:
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URIT‐8021A Service Manual

1) Adjust: To open the calibrating and testing interface


2) Arm raised: All probes and sticks are raised to the highest point.
3) Reset: All probes and sticks move down to corresponding washing pool; reaction tray, reagent tray and
sample tray move to the initial position and liquid path of washing pool begin to draw water.
4) Test: To perform the selected action
5) Save: To save all existing parameters.
6) Return: To exit parameter setup interface.

3.5.2 Setup of Motor Speed

Enter instrument parameter interface and click , the dialog box shown as figure 2­23 will pop

up

Figure 3­22 Speed Set Dialogue


It’s very important to check the parameters according to Parameter Table in accessories box because the
speed parameters determine the running speed of motors. Just maintain the default value if some
parameters do not listed in the table.

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URIT‐8021A Service Manual

NOTE
1) The actual value of each parameter is consist of two value in the case, such as 250 and
247, 250 represent big unit and 247 represent small unit(fine adjustment).The bigger the
value is , the faster the speed of motor is.
2) To setup the parameters in this interface according to Parameter Table.
3) The maximum speed is 255.

3.5.3 Parameter Setup of Liquid Path

1) Click to enter liquid path setup interface shown as figure3­23:

Figure 3­23
2) Check if the value of each parameter is consistent with the value in Parameter Table.
3) Each serial number in the interface is corresponded with the pump, valve or motor. The corresponding
value is the opening time of pump, valve or motor.
4) The right part of the interface is the function section; the unit of value is given in the bracket.
5) It is available for testing whether these components are connected properly. Input the corresponding
serial number and click Switch On, the corresponding pump, valve and motor will start to work. See
figure 3­24.

Figure 3­24

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URIT‐8021A Service Manual

NOTE
1) Take ms as unit for parameter which is not indicated unit
2) The serial number in front of each parameter represents the corresponding number of
motor.
3) Please consult the technician before performing fine adjustment of parameter
4) Click SAVE to save the data after adjusting.

CAUTION

Click to prevent probe collision when adjust the motion position.

Meaning of liquid path parameter


1) Spin cycle: The required times of instrument to complete a cycle procedure, measured by second.
2) Cuv.Path Length: The linear distance between two sides of cuvette which the light throuth, measured
by um.
3) Air Before Reg: The quantity of inhalation of air before aspirating reagent, measured by .
4) Air After Reg: The quantity of inhalation of air after aspirating reagent, measured by .
5) Reagent remainder: The quantity of aspirting reagent for rinsing the aspirating probe, measured by .
6) Extra R.volume: The quantity of aspirting reagent added to cuvette, and for calibrating the difference
value between actual volume and theory volume. Measured by .
7) Air Before Sam: The quantity of inhalation of air before aspirating sample, measured by .
8) Air After Sam: The quantity of inhalation of air after aspirating sample, measured by .
9) Sample remainder: The quantity of aspirting sample for rinsing the aspirating probe, measured by .
10) Extra S.volume: The quantity of aspirting sample added to cuvette, and for calibrating the difference
value between actual volume and theory volume. Measured by .

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3.5.4 Setup of Initial Position

Figure 3­25 Initial Position of Motion Component


1­No.35, Square Wiper 6­No.81 light spot
2­Adjusting left/right for optocoupler 7­No.44, the first nozzle initial position
3­ Adjusting front/rear for optocoupler 8­ optocoupler mounting bracket
4­ No.1, aspirating probe initial position 9­Light source
5­ No.110, stirrer initial position

1) Initial Position of Reaction Tray:

l Click in instrument parameter interface. Observe the


position of light spot when reaction tray stops, if it is not at the middle of cuv.81; adjust the left/right
optocoupler mounting bracket as figure 3 ­25, 3­26, 3­27.

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Figure 3­26 Adjusting front/rear position for optocoupler

Figure 3­27 Adjusting left/right position for optocoupler


NOTE
If the light spot located on the middle of cuvette, the position is right.

2) Initial Position of aspirating probe:

l On the R.probe set section, click . When


the mechanism unit returns to initial position of optocoupler, adjust the front/back and left/right position
of aspirating probe manually to make sure the aspirating probe is above the centre position of Cuv.1,
see as figure 3­28, 3­29. It is available to perform fine adjustment after packing the cover of sampling
arm.

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URIT‐8021A Service Manual

Figure 3­28

Figure 3­29

l Select and input the set parameter 50, then

click . Perform fine adjustment (the range of fine adjustment is 1 to 5) and observe
the position of aspirating probe in cuv.1. If it is proper, down the aspirating probe till the tip touches the
bottom of cuvette through gently pressing the arm of aspirating probe.
l When the tip is near the bottom of cuvette, please operate the align function step by step. The region of
fine adjustment shown as figure 3­30:

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URIT‐8021A Service Manual

Figure 3­30 Region of Fine Adjustment


NOTE
There are 2­3mm buffer course of anti­collision mechanism of aspirating probe. Observe whether
the optocoupler barrier is moving or not for adjusting depth.

3) Initial Position of Stirrer

l On the Stirrer set section, click . Adjust the


left/right and front/rear adjustment assembly to make sure that the stirrer is above the centre of cuv.110.
See figure 3­31

Figure 3­31

l Select and input the set parameter 50, then

click , perform fine adjustment (the range of fine adjustment is 1 to 5). Upward
moving 2 to 3 step when the stirrer reaches the bottom of cuvette.

4) Initial Position of Washing Hand


NOTE: It is not necessary to adjust the initial position of washing hand since it has been done before
delivery.
l Keep the position of reaction tray, turn washing hand to make sure the arrange radian of washing
probes are consistent with reaction tray. And then to adjust whole position of washing hand to make
sure the scrape block is above the centre of cuv.35.

l Click and input the set parameter 50, then

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URIT‐8021A Service Manual

click , perform fine adjustment (the range of fine adjustment is 1 to 5), descending
step by step. The requirement of adjusting washing hand as following:
a) The first to sixth porbe’s height is the same, the seventh and eighth are the same and deeper 1to 2
mm than the former.
b) scrap block is not permitted to touch the wall of cuvette(perform fine adjustment of radian or turn
rotate probes), see figure 3­32:

Figure 3­32
c) The spring anti­collision assembly of seventh and eighth probes raised 1 to 2mm (2 to 3 steps), and
others do not raised. See figure 3­33:

Figure 3­33
Scrap block is not permitted to touch the wall of cuvette during the adjusting course. Adjusting the
position of washing hand must be done if the scrape block touch the wall and diverge the cuvette. (the
position of washing hand has been adjusted before delivery, it is not necessary to adjust on the scene).
The adjusting methods are as follows: keep the position of reaction tray and loose four locking screws.
Then adjusting whole position of washing hand to make sure scrape block is above the centre of cuvette
35.

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Figure 3­34

3.5.5 Adjusting Position Parameter to Washing Pool

1) Reagent probe to washing pool:

l Select and set turning parameter as 45,

click and make aspirating probe above the washing pool. Perform fine adjustment
to adjust left/right of aspirating probe to make sure it is above the centre of washing pool.

l Select and set the vertical as 50, then click ,


adjust aspirating probe down to a position in washing pool which is easy to observe, then

click to adjust aspirating probe above the centre of washing pool.

l Select , then perform fine adjustment (rang of fine adjustment is 1

to 5) to make the inclined part of sampling part and up edge of inner washing cup at same horizontal line.
See figure 3­35:

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URIT‐8021A Service Manual

Figure 3­35
2) Stirrer to washing pool:

l Select and set turning parameter as 80, click


to make stirrer above washing pool. Perform fine adjustment to adjust left/right of stirrer to make sure
stirrer is above the centre of washing pool.

l Select and set parameter as 50 firstly, click


and adjust stirrer down to a position in washing pool which is easy to observe, then

click to adjust stirrer above the centre of washing pool.

l Select perform fine adjustment (rang of fine adjustment is 1 to 5).


When the up edge of stirring paddle is higher (about 3 to 5mm) than the up edge of inner washing cup.
See figure 3­36.

Figure 3­36

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3.5.6 Adjusting Position of Reagent /Sample Tray

1) Click , the reagent tray will move to No. 1 reagent postion.

l Select and set parameter as 110, then click to make aspirating


probe at the above of reagent bottle which located in No.1 reagent position. Adjusting aspirating probe
at the above of the centre of No.1 reagent position.

l Select and set parameter as 50, click and


adjust reagent probe down to a position in reagent bottle which is easy to observe, then

click to adjust aspirating probe above the centre of reagent bottle.

l The position of aspirating probe to reagent tray could be adjusted after performing the above processes.
If a large deviation still exist, the initial position of reagent tray should be adjusted. The adjusting
methods are as follows:
Loose three locking screws on the reagent tray bracket, and then move the reagent tray to make sure
the aspirating probe at the above of the centre of No.1 reagent position. See figure 3­37:

NOTE: It is not necessary to adjust the initial position of reagent tray since it has been done before
delivery.

Figure 3­37

l Select and set parameter as 50, click . Then


perform fine adjustment (rang of fine adjustment is 1 to 5), upward moving 1 to 2 steps when the
aspirating probe touch the bottom of reagent bottle.
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2) Click , the reagent tray will move to No. 31 reagent postion.

l Select and set parameter as 90, click to make aspirating probe at


the above of reagent bottle which located in No.31 reagent position. Adjusting aspirating probe at the
above of the centre of No.31 reagent position.

l Select and set parameter as 50, click and


adjust aspirating probe down to a position in reagent bottle which is easy to observe, then

click to adjust reagent probe above the centre of reagent bottle. The aspirating probe
could move down to the bottom of reagent bottle and keep the probe do not collide reagent bottle since
vertical of inner and outer reagent bottle are the same.

3) Select to make No.1 sample cup to the initial position.

l Select , make aspirating probe to the above of


No.1 sample cup and adjusting left/right to make sure the aspirating probe at the above of the centre of
No.1 sample cup.

l Select and set parameter as 50, then

click . Adjust aspirating probe down to a position in the sample cup which is easy to

observe, select and adjust left/right to make aspirating probe at


the above of the centre of sample cup.

3.5.7 Debugging of Optical Path

1) Wait for at least 30 minutes to make sure the light source is stable after power on. Then click

to enter cuvette signal interface, click , 120 cuvettes will be added water by
washing hand.

2) Click in the instrument parameter interface to make the reaction tray move to the

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initial position. Then click , the interface is displayed as figure 3­38:

Figure 3­38
3) Adjusting the potentiometers on the signal board to make the A/D value of corresponding wavelength is
in the range of 56000 3000. See figure 3­39

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Figure 3­39

4) Click to enter the interface shown as figur3­40. Then click , then the
interface will show the A/D vaule of 120 cuvettes after reaction tray stop.

Figure 3­40
5) Observe the A/D value and absorbance value. If the A/D value or absorbance is out of range, please
replace the corresponding cuvette to meet the requirements. Then click SAVE, and the debugging of
optical path is complete.

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CHAPTER 4 MECHANICAL UNIT MODULE


4.1 Shell and Structure of the Machine

This section introduces the internal structure of the machine, shell’s composition, remove and installation.

Figure 4­1 Analyzer

4.1.1 Shell

The shell of URIT­8021A is mainly composed of the protective cover, panel, left/right cover plate, front shell,
rear baffle, rear cover plate and cabinet. See figure 4­2:

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URIT‐8021A Service Manual

Figure 4­2(a)

Figure 4­2(b)

4.1.2 Disassembly and Instructure

It’s recommended that the analyzer should be repaired by professional personnel if the malfunction cannot
be solved according to the adjustment of software and routine maintenance. In case replacing a component
is needed, please following the steps:
1) Turn off the testing power switch.
2) Turn off the mian power switch.
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URIT‐8021A Service Manual

3) Remove the end of gas springs, then take down two hinges to remove the upper cover.(In general the
upper cover don’t need to remove)
4) Loose the six hexagonal socket head cap screws on the left and right sides of the front shell, and 5
screws on the front of panel, then pull out the front shell. Drive board, optical box, injection pump could
be seen. Seen figure 4­3:

Figure 4­3
5) The installation plate fixed in the frame by two hinges and a socket head cap screw. Loose the retaining
screw and open the installation board. Main board, power board and terminal board are installed on the
back of installation board. See figure 4­4:

Figure 4­4
6) The rear baffle is used for protecting liquid path. See figure 4­5:

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URIT‐8021A Service Manual

Figure 4­5
The liquid path could be seen when removing the rear baffle. See figure 4­6:

Figure 4­6
1­Pump for supplying distilled water 4­Distilled water tank
2­Pump forsupplying detergent 5­Drain waste solution negative pressure tank
3­Pump for draining waste solution

7) After removing rear baffle, loose the screws on the rear cover plate, then down open the rear cover plate,
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URIT‐8021A Service Manual

the inner instructure of instrument could be seen as figure 4­7:

Figure 4­7
8) Loose four M4 10 screws to remove the left cover plate. Then reagent tray refrigeration assembly(left),
reagent/sample tray, reagent temperature sensor could be seen, see figure 4­7:

Figure 4­8
9) Remove the right cover plate, reaction, power supply box, optical box and a part of washing hand, see
figure 4­9:

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URIT‐8021A Service Manual

Figure 4­9
10) The panel consists of four panels and two tray covers, shown as the figure 4­10. The disassembly
sequence of panel is: reaction tray cover reagent /sample tray cover arc middle panel B arc
middle panel A arc left panel arc right panel.
NOTE
Do not touch the aspirating probe and stirrer when disassembly panels.

Figure 4­10

After removing all plates and panels:


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URIT‐8021A Service Manual

Figure 4­11

4.2 Aspirating Unit

4.2.1 Function

Sample probe and reagent probe share a aspirating probe in aspirating unit. The mainly function is aspirate
the sample from sample cup (tube) to inject into cuvette and the operation of reagent is the same as sample.
And it’s also provide liquid level sensing, aspirating probe anti­collision, with the amount of tracking,
mechanical limit, power off protection etc.

4.2.2 Structure

Aspirating unit consists of drive mechanism and sampling arm. See figure 4­12:

Drive mechanism used for supporting sampling arm and making aspirating probe to vertical and horizontal
rotation. The drive mechanism is composed of vertical and rotary movement mechanism which are includes
stepping motor, synchronization belt and synchromesh gear, to perform the movement of probe by spline.

Sampling arm is composed of aspirating probe, liquid level sensing board, sampling arm cover and retaining
screw of aspirating probe, which supported and connected by sampling arm bracket. See figure 4­13:

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URIT‐8021A Service Manual

Figure 4­12

Figure 4­13

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4.2.3 Aspirating probe Installation

Figure 4­14
1) Remove the sampling arm cover, and then loose the retaining screw and spring to take down the
aspirating probe since it’s fixed on the sampling arm bracket.
2) Loose two screws that fixed on the spline to take out the sampling arm bracket.
3) Sampling mechanism installed on baseboard by four inner hexagonal screws, loose the screws to take it
out.
4) The installation steps are contrary to disassembly.
CAUTION
1) Checks the movement of aspirating probe whether is smooth or not after strengthen the
retaining screw, if not, loose the retaining screw to adjust.
2) Keep the surface of aspirating probe clean when disassemble the mechanism.
3) Disconnect the relative electric circuit and liquid path before disassembling the mechanism.

4.3 Stirrer Unit

4.3.1 Function

The stirrer has the functions of mixing the sample and reagent in cuvette, mechanical limit, Power off
protection.

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4.3.2 Structure

Stirrer unit is composed of drive mechanism and stirring arm. The main structure is shown as figure 4­15.

Drive mechanism is used for supporting stirring arm and making stirrer to vertical and horizontal rotation.The
drive mechanism is composed of vertical and rotary movement mechanism which are includes stepping
motor, synchronization belt and synchromesh gear, to perform the movement of probe by spline.

Stirring arm is composed of stirring assembly and stirring arm cover, which supported and connected by
stirring arm bracket. See figure 4­15:

Figure 4­15

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4.3.3 Stirrer Installation

Figure 4­16
1) The stirrer assembly consists of stirring motor, stirrer and micro adjustment plate, and fixed on the
stirring bracket by two screws. Remove the stirring arm cover, and then loose two retaining screws on
the micro motor adjustment plate to take down the stirrer.
2) Loose two screws that fixed on the spline to take out the stirring arm bracket.
3) Stirrer mechanism installed on baseboard by four inner hexagonal screws, loose the screws to take it
out.
4) The installation steps are contrary to disassembly.
CAUTION
1) When installing stirrer assembly, please downwards into the position hole.
2) Keep the surface of stirrer clean when disassembling the stirrer assembly.
3) Disconnect the relative electric circuit before disassembling the mechanism.

4.4 Reagent /Sample Tray Unit

4.4.1 Function

Reagent/sample tray is a container to load sample and reagent, and transfer accurately them to
corresponding position in sequence. The mianly function as follows:
1) Load reagent/sample: The sample cup (tube) with sample placed on the sample tray, and reagent
bottle with reagent placed in the reagent tray.

2) Rotate in sequence: Reagent/sample tray rotates in the sequence that setted by software, transfers

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URIT‐8021A Service Manual

the sample and reagent to aspirating position.

3) Reagent refrigeration: Provide and keep refrigeration temperature.

4.4.2 Instructure

Reagent/sample tray unit is divided into sample tray bracket, reagent tray bracket, reagent/sample tray drive
assembly, refrigeration module and reagent/sample tray thermostat assembly. The description of structure
as following:
1) Reagent/sample tray bracket fixed on reagent/sample drive assembly.

2) Reagent/sample drive assembly fixed on reagent/sample tray thermostat assembly.

3) Reagent/sample tray thermostat assembly fixed on refrigeration module.

4) Refrigeration module fixed on baseboard.

5) Sample tray bracket: load sample cup (tube), single ring, 45 sample positions. Controlled to rotate in
sequence by sample tray assembly.

6) Reagent tray bracket: load reagent bottle, includes outer and inner rings, each ring contains 30 positions,
and 60 reagent positions in total are provided by two rings. Controlled to rotate in sequence by reagent
tray assembly.

7) Reagent/sample tray drive assembly: controlled by software, transfer accurately sample/reagent to


aspirating position. Composed of drive bearing, coded disc, optocoupler, stepping motor,
synchronization belt and synchromesh gear.

8) Refrigeration module: for reagent refrigeration to make reagent meet the requirement of refrigeration
temperature, and keep stability of reagent. It’s consists of two fancooled refrigeration assemblies and
reagent/sample tray drive assembly.

9) Reagent/sample tray thermostat assembly: Protect the reagent/sample tray and keep the temperature
of reagent tray.

4.4.3 Install and Disassemble Reagent/Sample Tray

There are two types of reagent/sample trays in URIT­8021A:


1) Split reagent/sample tray: reagent and sample trays are separated, and each of trays is driven by one
motor assembly.
2) Integrative reagent/sample tray: sample and reagent tray brackets are combined and driven by one
motor assembly. See figure 4­17, 4­18, 4­19:

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Figure 4­17 Sample tray bracket Figure 4­18 Reagent tray bracket

Figure 4­19 Integrative Reagent/Sample Tray

4.4.3.1 Install and Disassemble Reagent/Sample Tray Bracket

The disassembly steps of split reagent/sample tray are as following:


1) Loose the retaining screw to take out reagent tray bracket.
2) Loose six screws in the position hole to take out the sample tray bracket.
3) The installation steps are contrary to disassembly.

The disassembly steps of integrative reagent/sample tray


1) Loose the retaining screws to take out the reagent/sample tray bracket.
2) The installation steps are contrary to disassembly.

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4.4.3.2 Install and Disassemble Reagent/Sample Drive Assembly

l Split reagent/sample tray drive assembly. See as figure 4­20, 4­21:


1) Refer to 4.4.3.1 to disassemble reagent/sample tray bracket.
2) Loose the screws on bracket adjustment plate to take out the plate.
3) Remove four screws on the left/right of reagent/sample tray thermostat assembly to take out
reagent/sample thermostat and drive assembly.
4) The installation steps are contrary to disassembly.

Figure 4­20

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Figure 4­21 Split Reagent/Sample Tray


l Integrative reagent/sample tray drive assembly:
Disassembly steps are the same as split reagent/sample tray. See as figure 4­22, 4­23

Figure 4­22

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URIT‐8021A Service Manual

Figure 4­23

4.4.4 Install and Disassemble Refrigeration module

1) Refer to 4.4.3 to disassemble thermostat and drive assembly.


2) Loose three screws on the peltier clamp, and then take out peltier clamp and heat­insulating block.
3) Pull peltier wire to take out peltier.
4) Loose four screws that fixed on the radiator from the rear baseboard, then take radiator out.
5) The installation steps are contrary to disassembly.

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Figure 4­24 Fancooled Refrigeration Assembly


CAUTION
1) The upper and lower surface of peltier shall not be installed in reverse, otherwise, peltier
does not refrigerate.
2) Tightening alternatively the screws which fix peltier clamp when install peltier to avoid
uneven stress on refrigerating effect.
3) Disconnect the relative electric circuit before disassembling the assembly.

4.5 Reaction Unit

4.5.1 Function

Reaction tray is holds cuvettes and provides a steady environment for biochemical test, which transmits
cuvettes to corresponding position in the sequence setted by software. It’s also cooperates with aspirating
probe, stirrer, washing hand and optical system to work.

4.5.2 Structure

Reaction tray is composed of cuvette holder assembly, constant­temperature incubation slot and reaction
tray drive assembly.

Cuvette holder assembly: it holds cuvette and consists of six groups of cuvette holders, 120 cuvettes and a
reaction tray cover. Reaction tray cover is installed on reaction tray drive assembly by nut cap.

Constant­temperature incubation slot: it’s installed on fitting seat by four inner hexagonal screws. Heating
strip is sealed in slot by cement gel, which connects to socket by a temperature switch.

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Reaction tray drive assembly: accurately control the movement of reaction tray. It’s composed of stepping
motor, optocoupler, coded disc, helical gear, bearing and reaction tray baseboard.

4.5.3 Install and Disassemble Reaction Tray

1) Loose the fitting nuts on a group of cuvette holder, then take out the holder and cuvettes to vacate a
place. Manually rotate the reaction tray to make washing hand is above this place.
2) Loose nut cap to take out reaction tray cover and cuvette holders.
3) Remove the screws on constant­temperature incubation slot; disconnect the circuit of temperature
switch, then take out constant­temperature incubation slot.
4) Loose the screws on baseboard to take out reaction tray drive assembly.
5) Installation steps are contrary to disassembly.

Figure 4­25 Structure of Reaction Tray

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4.6 Washing Hand Unit

4.6.1 Function

The function of washing hand is vertical moving to clean the cuvettes automatically.

4.6.2 Structure

Washing hand unit is composed of washing hand and drive assembly. Washing hand is fixed on the drive
assembly by metal thumbscrew and drive assembly fixed on baseboard.

4.6.3 Installation and Disassembly

1) Loose four screws on thumbscrew to take out thumbscrew.


2) Upward remove the washing hand.
3) Loose the clamping block to remove washing arm.
4) Loose three screws between drive assembly and reaction baseboard to remove drive assembly.

Figure 4­26
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4.7 Photoelectric Detection Unit

4.7.1 Function

Photoelectric detection unit is one of the centre units which directly determines the accuracy and precision of
analyze. It’s to produce light, split light, receive signal and convert light signal into electrical signal.

Optional wavelength: 340nm, 405nm, 450nm, 492nm, 510nm, 546nm, 578nm, 630nm, 700nm, 800nm
Accuracy of wavelength: 2nm
Linearity range: 0 to 3.0Abs
Resolution: 0.0001Abs
Light source: halogen lamp, 12V/20W.

4.7.2 Structure

The photoelectric detection unit is a whole sealing, statics, array and rear spectrophotometry optical system.
It’s mainly composed of light source holderassembly and optical box assembly. See as figure 4­27:

Figure 4­27 Optical Box


The halogen light generates steady continuous spectrum, suitably focus the light by convex lens and
through the cuvette, irradiate on quasi value mirror; the light will focus again by quasi value mirror and then
irradiate on monochromator.

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There are ten potentiometers in the right open slot of optical box for adjusting bright value. Ten
potentiometers are corresponded to 10 wavelengths respectively from up to down, they are 340nm, 405nm,
450nm, 492nm, 510nm, 546nm, 578nm, 630nm, 700nm and 800nm.

The principle of monochromator is as follows:


1) The continuous spectrum through the cuvette and then across quasi value mirror (entrance mirror) into
rtesting system.
2) The spectrum with specific wavelength (other spectrums are assigned to the next beam splitter) through
beam splitter and splitting by optical filter, the spectrum with single wavelength will be filtered, then
transformed and enlarged by transducer, at last output electric signal for testing.
3) The absorption signal of all wavelengths of liquid in the cuvette will be read and processed by the above
steps. Please see figure 4­28 for learning inner optical structure.

Figure 4­28 Optical Structure

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4.7.2.1 Light Source Holder Assembly

Figure 4­29 Structure of Rice Bulb Component

Figure 4­30 Light Source Holder Assembly

Figure 4­31 Rice Bulb Holder Figure 4­32 Halogen Light

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4.7.2.2 Optical Box Assembly

Figure 4­32

4.8 Install and Disassemble Injection Pump

The injection pump (500 ) located in the front of analyzer, with the left solenoid valve and aspirating probe
composes sampling assembly that fixed on the instrument by a mounting plate. The disassembly steps are
as following:
1) Screw out the black connectors on the injection pump.
2) Loose socket head cap screws to take out the injection pump.
3) The installation steps are contrary to disassembly.
CAUTION
1) Two black connectors should be connected to corresponding interfaces.The connector
which connects with solenoid valve is connected to the interface in the middle part of
injection pump, and the other one connected to the interface on the top.
2) There is a green collar inside the black connector to connect the Teflon pipe. Ensure the
mouth of teflon pipe parallel to the face of collar and should not extend or retract, otherwise
the leakage of aspirating probe may be occured.

Figure 4­34
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CHAPTER 5 LIQUID PATH

5.1 Function

1) Liquid path system is divided into two parts: sample aspirating and washing.
2) The part of aspirating sample consists of a aspirating probe, injection pump (500 ), solenoid valve and
stirrer, which provided by distilled water tank. It flow through solenoid valve and open to the 500
reagent injection pump then open to aspirating probe to wash the inner wall of probe.
3) The part of washing includes distilled water supply and waste solution drainage.
l Distilled water is draw to distilled water tank, then generate positive pressure. If the pressure is out of
the range that pressure switch provides, the pump and valve will be turned on to supply water.
l Drain waste solution pipeline is divided into two parts: motive power drainage and gravity drainage.
Aspirating probe washing pool and stirrer washing pool waste solution drainage, and reagent
condensated water drainage are gravity drainage that draw waste by it’s gravity. All waste solution
drainages are motive power drainage except washing pool in the instrument. Waste solution pump
generates motive power to make waste solution vacuum tank into vacuum negative pressure. All
pipelines are controlled by solenoid valve. When the pipline to draw waste solution into waste solution
negative pressure tank, the corresponding solenoid valve is turned on. Finally, waste pump drain the
waste solution from instrument.
4) Washing pool for washing the inner and outer walls of aspirating probe, and stirrer with distilled water,
then the waste will be draw into waste solution barrel.
5) Washing hand is to clean cuvettes automatically which composed of eight groups of probe, the front six
groups are double­probe, and the other two groups are signal­probe.
l The first group is detergent probe, the short probe is for injecting detergent controlled by individual
motive pump and valve, the long probe is for drawing waste solution to waste negative pressure tank by
vacuum pump.
l The groups of second to sixth are the distilled water probe. The long probes is for drawing waste
solution to waste pipe by vacuum pump; Five short probes is injecting distilled water, the motive power
is provided by water adding vacuum pump, a liquid separate box is used for separating distilled water
into five ways. Furthermore, a pump back system is setted for preventing liquid dropping from short
probes. A pump back solenoid valve control the system, both ends of solenoid valve connect with
distilled water injecting pipeline and waste solution negative pressure tank respectively. When the
solenoid valve is open, both ends is connected, the excess distilled water in probe will be draw by
negative pressure.
l The long probes of first to seventh groups drain distilled water and collect to a pipeline by a solenoid

valve.

l The eighth group is the drier probe which controlled by a individual solenoid valve.

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Figure 5­1
6) Washing pool liquid path is divided into two paths and each of them controlled by a solenoid valve to
provide distilled water for washing pool to rinse aspirating probe and stirrer respectively. And all waste
will be collected to a pipeline and then flow to waste solution barrel by it’s own gravity.
7) There is an exhaust valve to keep the gas and liquid in distilled water tank balance, and drain excess
gas.
8) The waste solution from washing pool and condensated water from reagent tray are drained by it’s own
gravity.

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5.2 Liquid Path Diamgram

Figure 5­2

5.3 List of Valve and Pump


List of valve and Pump
No. Function
V1 Contol liquid path to washing the inner wall of aspirating probe
V3 Solenoid valve for supplying detergent to washing hand(Hand1)
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V4 Solenoid valve for supplying distilled water to washing hand(Hand2­6)


V5 Solenoid valve is control washing hand to inject and draw water (Hand2­6)
V6 Solenoid valve is control washing hand to draw waste solution (Hand1­7)
V7 Solenoid valve for supplying distilled water to washing pool of aspirating probe
V9 Solenoid valve for supplying distilled water to washing pool of stirrer
V10 Solenoid valve is control washing hand to draw waste solution
V11 Solenoid valve is control distilled water tank to drain gas
V12 Solenoid valve for supplying distilled water to distilled water tank
P1 Pump for supplying detergent
P2 Vacuum pump for supplying distilled water
P3 Negative pressure tank vacuum pump
SP1 Aspirating probe injection pump
T1 Distilled water tank (Positive pressure)
T2 Wast solution negative pressure
Rc1 Washing hand liquid separate box
Rc2 Wate solution collect box
M Washing pool of stirrer
R Washing pool of aspirating probe
WH Washing hand
PS Pressure switch(ON:0.10MPa OFF:0.12MPa)
DT Plug
K1 Adaptor
Y70_1­ Y70_3 Tee
J1­J7 Adaptor(Plastics)

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CHAPTER 6 ELECTRONIC CIRCUIT OF HARDWARE

6.1 Summary
This chapter mainly introduces the connecting methods and principles of circuit and function of each circuit
board, etc.

6.2 List of Circuit Board


Name and function of circuit board of URIT­8021A are as follows:
List 6­1 Circuit board of hardware
No. Name Function
Main board is the controlling core of analyzer. It communicates with
1 Main board computer with RS232 and controls the actions of lower­position
machine, receiving and collecting data and signal.
The function of Drive board is to drive and control each motor, on­off
2 Drive board
control of pump and valve.
3 Terminal board mainly centralized control power supply

4 Power board Supply power to each circuit board of lower position machine.

Transfer board of Receiving signal of Drive board and control on­off of each pump and
5
pump and valve valve.
The function of signal amplify board is to collect light signals, adjust
6 Signal amplify board and do A/D signal transition, then supply the transferred signal to main
board.
liquid level sensing Providing function of liquid level sensing and anti­collision for
7 board of asipirating aspirating probe
probes
Mainly receiving driven signal of controlling motor and transfer
optocoupler signal to Drive board. (Includes transfer board of
Transfer board of
8 aspirating mechanism, transfer board of reagent/sample tray, transfer
optocoupler of motor
board of injection pump, transfer board of stirring mechanism, transfer
board of washing hand and reaction tray.)
Transfer board of Supply power to transfer light.
9
light

6.3 Position of Circuit Board


The position of each circuit board as follows: 6­1, 6­2, 6­3, 6­4.

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Figure 6­1 Drive board, signal amplify board

Figure 6­2 Main board, power board, terminal board

Figure 6­3 Transfer board of pump and valuve, optocoupler transfer board of motor

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Figure 6­4(A)Liquid level sensing board Figure 6­4(B)Light transfer board

6.4 Principle of Circuit Board

Refer to appendix A for principle and connection of circuit board of URIT­8021A.

6.5 Function of Circuit Board

6.5.1 Main board


The main board is the control center of lower position machine. PC send controlling information by RS232,
and the information is sent to U16 (RS232 chip) by JP4 interface, then sent to CPU for processing. CPU
controls the action of reaction tray directly. Control signal is sent to PRT module to control the action of
motor by U6 (signal is strengthen and reverse) and P88 plug. Other motors, pumps and valves are
controlled by controlling information which send from P88 to the modules of main board.
Signal of AD value of signal board is sent to main board pass through PAMP signal plug, pass through U5
and U2 then sent to CPU, CPU sent signal back to PC. Please refer to figure 6­3 and 6­5 for voltage test
points and line connection of main board.
Figure 6­2 Table of comparision of integrated chip of main board
Position of main board Chip No. Function of chip
U19 SP×1117M3­L­3­3 1.0A low power and low dropout regulator
U1 REF3140 A/D conversion chip provides 4.096V reference voltage
U5 OPA365 High­precision operational amplifier, as follower in circuit
U10 74HC14 Hex Schmitt trigger
U6 CD74HC24OM Bumper/Line Driver
U9 MA×813L Reset, Watch dog and Power monitor chip
U20 MA×809SEUR Reset Circuit
U16 SP3223EEY MA×232
U3 MA×3080CSD RS485 transceiver,receive threshold ­200mv,­50mv
U2 MA×113 200k/s,16 digit AD analog­to­digital conversion
UP1 SRV05­4 Data line and port protection

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U8 C805IF120 Single Chip(Silicon Labs)

Figure 6­3: Voltage of test point of main board


Test point Voltage range(V)
+5V 5±0.1
+3.3V 3.3±0.1
U5(Pin 4) ­0.33±0.05
C15(under capacity ) 4.096±0.01

Note: pin 13 and


14 are not used

Power of
5V power main board
【DY5V】 (PO1)

4.096V
test point

DB.ZG
The connector
for connecting
signal amplifer
board.【DB.ZG】

Waste
solution
3.3V test BNC
(JP9)
point 【FBNC】

5V test Detergent
point BNC
( JP8)
【QBNC】

Distilled
waterBNC
(JP7)
DB.ZQ 【ZBNC】

The connector for connecting


drive board【DB.ZQ】 RS232 (JP4)【RS232】

Figure 6­5 Main board connecting and test point

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NOTE
The content in bracket is the code of plug.
Power on after connecting power board; test the voltage of each point by using multimeter. Check circuit and
components if the voltage is not in the range. Please see figure 6­3 for voltage range of test point of main
board.

U5 chip needs positive and negative voltage, positive +5V, negative ­0.3V. 4.096V is the reference voltage
of transfer chip.

Figure 6­6 Diagram of main board

6.5.2 Main Board

Drive board mainly achieve driven of each motor, it is divided into eight modules, one T module, six driven
modules (A to F) and one module for controlling pump and valve.

The main board sends instruction to drive board by P1; the driven part of reaction tray of T module is
controlled by CPU directly. The controlling signal of washing hand and other six modules is gained by 485
Bus. The T module only control the motor of washing hand which driven the up and down action, other six
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modules could driven two motors.

The work processing of drive board is as following: CPU send action instruction of one motor to drive board
by 485 Bus. Through DIP address selection, the corresponding module processor 89S52 of drive board
receiving instruction, the output signal strengthen reversely through 74240, then be sent to driven chip
THB6064, so that to driven motor.
Refer to figure 6­4 for control details and DIP jumper setup.
Figure 6­4 Driven module division and DIP address jumper
corresponding
Driven transferring Setting No. DIP address
Motor Remark
module connection line of motor jumper
of motor
Motor of
Turn 0 Non­dial switch
reaction tray
T
Motor of
Lift 1 00001
washing hand

A not used

B not used

Motor of sample
Lift 2 00010
tray
C
Motor of reagent
Turn 7 00111
tray

Motor control up
and down action
Lift 8 01000
of aspirating
probe
F
Motor control
left and right
Turn 9 01001
action of
aspirating probe

Motor control up
and down action Lift 10 01010
D
of stirrer
Motor control Turn 11 01011

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right and left


action of
reagent stirrer

Sample
Lift 12 01100
injection pump
E
Reagent
Turn 13 01101
injection pump

Using multimeter to test voltage of each test point of drive board. See figure 6­7. Check circuit and
components if the voltage is not in the range. Refer to figure 6­5 for voltage range.
Figure 6­5 Voltage of test point of drive board
Test point Voltage range(V)
J—Power 24±0.7
Dr.Bd1 12±0.7
PJ11(voltage of pin 1) 12±0.7
P5 5.1±0.2
PJ4(voltage of pin 3) 12±0.3

Figure 6­5 Diagram of drive board

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6.5.3 Terminal Board

The function of terminal board is to supply power for analyzer.


Using multimeter to test output voltage of terminal board after power on. See figure 6­8 and 6­9. The voltage
range of terminal board is shown as figure 6­6.
Figure 6­6 output voltage of terminal board
Test point Voltage range(V)
AC1 220 10
SMPS1 220 10
ACB 220 10
ACD 220 10

Figure 6­8 Diagram of line connection of terminal board


NOTE
The content in bracket is the code of plug.

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Figure 6­9 Diagram of line connecting of terminal board

6.5.4 Power Board


Power board distributes the power to each part of lower position machine. There are four channels to output;
output of A channel is ±5V, B channel is ±5V, C channel is ±12V, the last is +5V. Test point is the interface of
pin 1 to pin 14. See figure 6­7. P1 is the switching power input, voltage is ±15V.

Test output of P1 by multimeter after connecting transformer. If the voltage is not in range, adjust
potentionmeter until it’s in the range. See figure 6­7 for output voltage of power board. See figure 6­10 and
6­11 for line connecting and voltage testing.
Figure 6­7: Output voltage of power board
Test point Voltage range(V)
JP4 12±0.05
P1 5V1 5.2±0.1
P1 5V2 5.1±0.1
P1 +12V 12±0.2
P1 ­12V ­12±0.2
P1 +5VA 5±0.1
P1 ­5VA ­5±0.1
P1 +5VB 5±0.1
P1 ­5VB ­5±0.1

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Figure 6­10 Test point of line connecting and voltage

NOTE
The content in bracket is the code of plug.

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Figure 6­11 Diagram of line connecting of power board

6.5.5 Transfer Board of Pump and Valve

The transfer board receives instruction which from pump and valve controlling module by JP25 to control
pumps and valves. Voltage of solenoid and vacuum pump is 24V. See figure 6­12 and 6­13 for line
connecting.

Voltage test of transfer board (motor/pump and valve):

Test the voltage of test point by using multimeter after connecting the lines of drive board. Check circuit and
components if the voltage is not in the range. See figure 6­8for voltage range of transfer board.
Figure 6­8: voltage of test point of transfer board
code Voltage range
24V 24±0.2V

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Figure 6­12 Diagram of line connecting of transfer board

Figure 6­13 Diagram of line connecting of transfer board of pump and valve

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6.5.6 Motor Transfer Board

The function of motor transfer board is to receive drive signal from drive board, then separate into two parts,
includes LIFT and TURN. Each part could supply separate interface for connecting line of motor and
optocoupler.

There are six motor transfer boards. Only aspirating probe with JP2 and JP3 plug which function is
anti­collision and liquid level sensing, the others have not.

See figure 6­9, 6­14, 6­15, 6­16 for detail line connecting method and voltage test point
NOTE
The content in bracket is the code of plug.

Figure 6­9: Voltage test point of moter transfer board


Code Voltage range
+5V 5±0.15V

Figure 6­14 Transfer board of aspirating probe

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Figure 6­15 Transfer board of washing station and reaction tray

Figure 6­16 Transfer board of stirring mechanism

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6.5.7 Signal Amplify Board

The function of signal board is to convert optical signal into electrical signal. Then amplify and transfer to
main board.
There are ten silicon photocells on signal board, the function of these photocells are to receive optical signal
and convert into weak voltage signal, then amplify the signal by IC LTC6244 (U1_S3 to U1_S12, each
photocell corresponds one piece of LTC6244), then amplify signal again by OPA365 (U2), select by DG506
(U3), at last transfer to main board by PAMP1. See figure 6­17 for line connection.

UT1 is power supply chip which voltage is ­5V; UT2 is power supply chip which voltage is 5V.

Test the voltage of test point by multimeter after power on. Check circuit and components if the voltage is not
in the range. See figure 6­10 for voltage range of signal board.
Figure 6­10 Voltage of test point of signal board
Code Voltage range
+5V 5±0.05V
­5V ­5±0.05V
+12V +12±0.2V
­12V ­12±0.2V
­0.3V ­0.3±0.05V

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Figure 6­17 Diagram of line connecting of signal board

NOTE
The content in bracket is the code of plug.

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Figure 6­18 Voltage test point on the back of signal board

6.5.8 Liquid Level Sensing Board

The liquid level sensing board consists of liquid level sensing part and anti­collision optocoupler.

Liquid level sensing adopts capacitive level induction principle. Probes link to U2 (MC3394) by JP2, and U2
converts capititance changes into voltage changes, then feedback to U1 (MC9S08SH8) for processing.
Alarming of reagent allowance could be achieved by U1.

The anti­collision optocoupler has no output voltage under normal condition. The optocoupler will get
through if probe collision. Pin 4 will feedback collision signal to drive board, then control signal will be sent
from drive board. Probe will uplift to the top automatically to protect probe.

Test voltage of test point by multimeter after connecting liquid level sensing line. See figure 6­19 and 6­20.
Check circuit and component if the voltage is not in the range. See figure 6­11 for testing voltage range of
liquid level sensing board.

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Figure 6­11 Voltage of test point of liquid level sensing board


Test point Voltage range(V)
﹢12V 12±0.7
VCC1(IC MC33941 Pin 12_ 5±0.1

Figure 6­19 Line connecting of liquid level sensing board and voltage test point (old version)

Figure 6­20 Line connecting of liquid level sensing board and voltage test point (old version)
NOTE
There is no obvious voltage test point on new version liquid level sensing board; its voltage is
changed by chip into 5V. If it is need to be test, please contact URIT.

The number of 1 to 4 is the label of jumper cap joint in figure 6­20; the jumper cap is used for programming
the procedure of liquid level sensing board and selecting sensitivity of liquid level sensing. See table 6­12 for
the function of jumper cap.
Table 6­12 Table of function of jumper cap group

Function of jumper cap


Selection of sensitivity of jumper cap
No. Function

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Joint for programming liquid Not plug jumper cap­­­the lowest


1
level sensing procedure sensitivity
2 Medium sensitivity joint plug No.3 jumper cap, low sensitivity

3 Low sensitivity joint No.2 jumper cap, medium sensivity

Joint for programming liquid Plug No.2+No.3 jumper, the most


4
level sensing procedure sensitivity

6.5.8 Light Transfer Board

The light transfer board is a “transfer stop” of light power line, which separate light and power and convinent
for replacing light.
See figure 6­21 for line connecting of light transfer board

Figure 6­21 line connecting of light transfer board

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CHAPTER 7 SOFTEWARE PARAMETER

7.1 General Parameter in Registry Table

7.1.1 Way to Entrance

Click Begin ­­­Run, input regedit, and then click confirm. Click my computer­­
HKEY_CURRENT_USER­URIT­DIAGNOSE; it is the location of saving of parameter.

7.1.2 Sign of Simulation Mode

In MotorSet parameter table, double click CRAZY_SIMΜLATION_MODE to entry to modify.


0 means normal motion mode, 1 means simulation mode.
NOTE
1) Under the working condition of simulation mode, the test speed will be accelerated and
virtual data will be created.
2) Do not use simulation mode when computer connect to analyzer, otherwise, will appear
probe collision.

7.1.3 Sign of Having Water in Cuvette

In System parameter table, double click HaveWater to entry.


0 means there is no water in cuvette; 1 means there is water in cuvette.

NOTE

System will prompt to drain water before test if there is water in cuvette.

7.1.4 Path of Software Registration

In System parameter table, double click Directory to entry. That is the registry path of software which must
be accordance with actual path, otherwise will make mistake.

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7.2 Common Files of Software

7.2.1 Main Procedure

Icon:
Function: Main control program

7.2.2 Test Program

Icon:
Function: Test control program

7.2.3 Data Base

Name: URIT­DIAGNOSE.mdb
Function: it is used for storaging all parameters, datas, include reagent parameter, test result, hospital setup,
QC and calibration datas, except mechanism parameter, instrument status and test curve.
Application: Copy this document before install new software, and replace the new document in new software,
so that the old data could be reserved.

7.2.4 Test Data Folder

Name: CheckData
Function: it is used for storaging test curve, a file is generated for one day,its name is the date when the file
is generated.
Application: The test curve data could be checked in different computer on URIT software after copying.

7.2.5 Language Package Folder

Name: Language
Function: it is used for storaging all text message, prompt information as Not found will appear if any loss or
damage.
Application: Modifying the text description according to requirement and the text will be displayed on
corresponding position.

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7.3 Method to Open Common Function

Different functions are configured for satisfying different requirements. A part of function is shielding since
different model with different hardware. The opening method and relative function are as follows:
Click D:\URIT Biochemical Analyzer, then open the Crazy.ini file by notepad or wordpad program.

1) Temperature displayed function


Discription: the temperature of reaction tray and reagent tray will be displayed by open this function
Character field: TemperatureDisplay
Opening method: after =, number 0 means closed, 1 means open.

2) Version reading and displaying function of lower position machine


Discription: this function is used for query program version of main board and low position machine.
Character field: VersionRead
Opening method: after =, number 0 means closed, 1 means open.

3) Cleaning function before test


Description: cleaning cuvette before test automatically
Character field: Clean BeforTestModule
Opening method: after =, number 0 means closed, 1 means open.

4) Automatic printing function


Description: the test data will be printed automatically after completing a test.
Character field: AutoPrintModule
Opening method: after =, number 0 means closed, 1 means open.

5) Alarming function when sample shortage


Description: alarming when sample shortage.
Character field: UseSamRegWarn
Opening method: after =, number 0 means closed, 1 means open.

6) Sample alarming information printing function


Discription: To print out sample shortage information with test result.
Character field: PrintSamRegWarn
Opening method: after =, number 0 means closed, 1 means open.
NOTE
1) Number 1 means open, 0 means closed, carefully execute this operation or contact URIT to
avoid instrument damage.
2) All the function must be matched with hardware. If not, instrument may be damaged after
power on.

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CHAPTER 8 MAINTENANCE
Please strictly according to the operating manual to operate and maintain instrument to guarantee the safety,
reliability and good working condition of instrument.
CAUTION
1) Please strictly according to the operating manual to operating instrument, otherwise damage
or personal injury will be occurred.
2) People who not trained or authorized by URIT, is not permit to repair the instrument.
3) Do not spill water, reagent and detergent on mechanical or electrical components.

Biological Hazard
Be sure to put on protective gloves, clothes, or even goggle if necessary.

8.1 Preparation
The following tools and detergent may be used in maintenance.

Tool
A set of hexagon wrench
Slotted point screwdriver (medium size)
Nozzle cleaner
Clean beaker
Tweezer
Clean gauze
Clean cotton swab
Brush (used for clean barrel)

Detergent
1) Alkaline detergent, concentrate detergent
2) Acid detergent
NOTE

1) The alkaline detergent is concentrate, dilute it with distilled water, the proportion is
1:9.
2) Please use the detergent supplied by URIT. If not, the test result is not reliable.

Others
Absolute alcohol
Clean beaker

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8.2 Daily Maintenance

Do the following steps everyday to keep instrument under good working condition.

8.2.1 Check Distilled Water

1) To make sure the testing switch is off.


2) To check allowance of distilled water in barrel
If the allowance is enough, do step 5.
If the allowance is insufficient, do next step.
3) Screw off the cover of distilled water barrel in an anticlockwise direction and injecting distilled water.
4) Screw on the cover of distilled water barrel in a clockwise direction.
5) Check the connection of pipeline of distilled water.
If there is leakage, press the circle and pull out distilled water pipe.

Figure 8­1 Pull Out Distilled Water Pipe


Check the pipe, if there is any damage, cut off the damaged part. If not, insert the pipe and check again.

Figure 8­2 Insert Pipe


6) Check the connection of BNC signal line: check the joint connect well or not.

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Figure 8­3 BNC Connection

If the connection is well, do the next step; if not, connect well as figure 8­3.

8.2.2 Check Detergent Barrel

1) To make sure the testing switch is off.


2) Check the allowance of detergent barrel.
If the allowance is enough, do step 8; if not enough, do the next step.
3) Screw off signal line in a clockwise direction.

Figure 8­4 Before Screwing Figure 8­5 After Screwing


4) Screw off the cover of detergent barrel in an anticlockwise direction; take down it with liquid level sensor.

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Figure 8­6 Screw Cover of Detergent Barrel Figure 8­7 Take Down Cover of Detergent barrel
NOTE
Put the cover with liquid level sensor on the clean desk.

5) Dilute concentrate alkaline detergent with distilled water according to the proportion, then pour into
detergent barrel.
6) Put on cover with liquid level sensor and tighten.
7) Connecting BNC signal line.
Insert BNC signal to the joint and tighten.

Figure 6­8 Before Screwing Figure 6­9 After Screwing


8) Check the connection of distilled water pipeline:
If there is leakage, press the circle and pull out distilled water pipeline.

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Figure 8­10 Pull out Detergent Pipe


Check the pipe, if there is any damage, cut off the damaged part. If not, insert the pipe and check again.

Figure 8­11 Insert and Tighten Distlled Water Pipe

8.2.3 Clean Waste Solution Barrel

Biological Hazard
1) Be sure to put on protective gloves, clothes, or even goggles if necessary.
2) Dispose the waste solution according to local discharge standard, and consult reagent
supplier or distributor.

1) To make sure the testing switch is off.


2) Check the allowance of waste solution, if it is too much, do the next step.
3) Pull out waste solution pipe: There are two waste solution pipes, the thick one flows low­concentration
waste solution, and the other (the thin one) flows high­concentration waste solution.

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Figure 8­12 Waste Solution Barrel


4) Pull out BNC signal line.

Figure 8­13 Hold the BNC Line Figure 8­14 Unscrew BNC Line

Figure 8­15 Pull Out BNC Line


5) Screw off cover of waste solution cover.
6) Discharge the waste solution into the specified pool.
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7) Insert waste solution pipe.


8) Connecting BNC line.
Insert BNC line into joint and tighten.

Figure 8­16 Hold the BNC Line Figure 8­17 Insert BNC Line

Figure 8­18

9) Screw on the cover of waste solution barrel in a clockwise direction.

8.2.4 Check Cleaning Position of Reagent and Sample Tray, Check Dilution Position

Check whether the detergent of No.60 position of reagent tray is enough or not, if not, sufficient it.
Check whether the detergent of No.59 position of reagent tray is enough or not, if not, sufficient it.

8.2.5 Check/Clean Aspirating Probes, Stirrer and Cuvettes

Check aspirating probes, stirrer and cuvettes. If there are any obvious stains, clean it according to weekly
maintenance.
Click Execute to perform probes and stirrer cleaning and cuvette rinsing after power on. See figure 8­19.

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Figure 8­19
Performing probe and stirrer cleaning, cuvette rinsing and add water before exit system. See figure 8­20.

Figure 8­20

8.2.6 Check Printer and Printing Paper

Check the work condition of printer everyday and make sure the printing paper is enough.

8.3 Weekly Mintenance

Do the following maintenance every week to keep analyzer in best work state.

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8.3.1 Aspirating Probes and Stirrer

CAUTION

Be careful to operate to avoid being scractched by the needle tip.

Biological Hazard
1) Be sure to put on protective gloves, clothes, or even goggles if necessary.
2) Do not discard gauze which used for cleaning aspirating probes. Dispose it according to
relative regulation.
1) To make sure the test switch is off.
2) Gently uplift the aspirating probe rocker to the highest point and turn it to a position where convenient for
operating.
CAUTION
Do not touch the surface of probes and stirrer with tweezer when clean to avoid scratch. And
avoid apply too much pressure to make probe and stirrer bending.

3) Pick up a piece of gauze which with absolute alcohol to wipe surface of aspirating probe and stirrer from
up to down. Especial to wipe the needle tip until the surface is bright and clean.

Figure 8­21 Clean Aspirating Probe

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Figure 8­21 Clean Stirrer

4) Switching on testing switch


5) Open system software and click Maintenance Probe and Stirrer Cleaning

Figure 8­22

6) Click RESET, aspirating probes and stirrer will turn to washing pool and washed by distilled water.

Figure 8­23

8.3.2 Clean Reagent Tray and Sample Tray

Biological Hazard

1) Be sure to put on protective gloves, clothes, or even goggles if necessary.


2) Do not discard gauze which used for cleaning aspirating probes. Dispose it according to
relative regulation.
1) To make sure the test switch is off.
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2) Loosen the two fixed screws on reagent and sample tray.

Figure 8­24
3) Uplift the reagent tray bracket and pull out.

Figure 8­25
4) Using a piece of gauze with absolute alcohol to clean the surface of reagent and sample tray and
screws of holder, until there are no stains on the surface of reagent tray.
5) Using a piece of gauze with distilled water to clean the surface of reagent and sample tray and screws of
holder, until there are no alcohol trace on the surface.
6) Install the reagent and sample tray, tighten fixed screw then put on cover.

8.3.3 Clean Washing Pool

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Caution
Be careful to operate to avoid being scractched by needle tip.

Biological Hazard
1) Be sure to put on protective gloves, clothes, or even goggles if necessary.
2) Do not discard the cotton swab which used for clean washing pool. Discord it according
to the relative regulation.

1) To make sure the test switch is off.


2) Gently uplift the aspirating probes and stirrer rocker to the highest point and turn to a position where
convenient for operating.
3) Using a piece of cotton swab with alcohol to clean surface and around of washing pool until there are no
stains.

Figure 8­26 clean washing pool Figure 8­26 clean washing pool
4) Switching on test switch.
5) Open system software and click Maintenance Probe and Stirrer Cleaning

Figure 8­27

Click Reset, aspirating probe and stirrer will turn to washing pool and cleaned by distilled water.

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Figure 8­29

8.3.4 Clean Reaction Tray

Biological Hazard

1) Be sure to put on protective gloves, clothes, or even goggles if necessary.


2) Do not discard gauze randomly, please dispose according to relative regulation.
1) To make sure the test switch is off.
2) Wipe the surface and around of reaction tray by gauze with distilled water until there are no stains.

Figure 8­30
CAUTION

DO NOT wipe reaction tray by gauze which with absolute alcohol or detergent since it will
cause corrosion of reaction tray. All losses thus incurred should borne by users.
CAUTION
The gauze should not with too much distilled water to prevent excessive water flow to cuvette.

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8.3.5 Clean Washing Hand

CAUTION
Be careful to operate to avoid being scractched by needle tip.

Biological Hazard
1) Be sure to put on protective gloves, clothes, or even glasses if necessary.
2) Do not discard gauze randomly, please dispose according to relative regulation.

1) Be sure the test switch is off.


2) Using a piece of gauze with distilled water to clean the surface of washing hand from up to down until
there are no stains.

Figure 8­31 Clean Washing Hand

CAUTION
Do not touches the surface of probe by tweezer to avoid scratch washing hand; and avoid
applies too much pressure to bend washing hand probe.

8.3.6 Clean Panel

Biological Hazard
Be sure to put on protective gloves, clothes, or even glasses if necessary.

Do not discard gauze randomly, please dispose according to relative regulation.

1) To make sure the test switch is off.


2) Using a piece of gauze with distilled water to clean panel, cover of reaction tray and reagent tray until
there are no stains.
CAUTION
The distilled water contain in gauze should not too much to avoid flow into crack of panel.

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8.3.7 Deep Clean Cuvette

Deep clean cuvette weekly to extend life and clean the deposited dirt on the inwall of cuvette.
1) Fill detergent barrel with detergent.
2) Click Maintenance Cuvette rinsing DeepClean.

Figure 8­32

8.3.8 Check AD Value and Save Cuvette Blank

1) Switching on test switch


2) Click Maintenance Cuvette signal.

Figure 8­33
3) Click Add Water

Figure 8­34
4) Click Cuv. Blank Reading
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Figure 8­35
5) Check the AD value in the list

Figure 8­36 Interface of AD value


Check the AD value of all cuvettes by clicking the arrows of left and right.

Figure 8­37
All the AD value should be in the range of 40000 to 60000.
Please refer to the relative contents in the chapter of Check/Change Cuvette if value not in the range.
6) Click SAVE to save cuvette blank.

Figure 8­38
7) Click Drain water to pump all water out.

Figure 8­39

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8.4 Monthly Maintenance

Do the followings maintenance every month to keep analyzer in best work state.

8.4.1 Clean Incubation Bath

1) To make sure the test switch is off.


2) Screw off the fixed screws on cuvette bracket.

Figure 8­40 Screw off Fixed Screws on Bracket


3) Take out cuvette bracket and put on clean desktop.

NOTE
Do not scratch the cuvtte and stain the cuvette when take out the cuvette bracket.

4) Take out cuvette bracket in sequence.

Figure 8­41 Reaction Tray

NOTE
The cuvette bracket which under the washing hand is not easy to take out. Rotate the
reaction tray to a proper position then take out.

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5) Using a clean gauze to clean the incubation slot.

Figure 8­42 Clean Incubation Slot

NOTE
Do not scratch the light hole when clean the incubation slot.

6) Put the cuvette bracket to the incubation slot, the


location hole fix to location pin.

Figure 8­43 Location Hole Figure 8­44 Loaction Pin

Figure 8­45 Two Sides in Level


7) Screw on fixed screws and tighten.
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8) To fix six cuvette brackets well in sequence.


9) Do the steps as 8.3.8 and save cuvette blank.

8.4.2 Clean Distlled Water Barrel

1) To make sure the test switch is off.


2) Pull out distilled water pipe.
Press the circle and take out distilled water pipe.

Figure 8­46 Pull Out Joint


3) Screw off BNC signal line

Figure 8­47 Hold BNC Signal Line Figure 8­48 Screw off BNC Line

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Figure 8­49 Hold BNC Line


4) Screw off cover of distlled water barrel.
5) Pour out the rest distilled water.
6) Clean the inwall of distilled water barrel, using a brush to clean if necessary.

NOTE
Do not scratch the liquid lever sensor, catheter and filter when using a brush to clean.

7) Using a clean gauze to clean the external and cover of distilled water barrel.
8) Insert distilled water pipe.

Figure 8­50 Insert Pipe


9) Connecting BNC line.
Insert BNC signal line and tighten.

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Figure 8­51 Hold BNC Line Figure 8­52 Screw off BNC Line

Figure 8­53 Hold BNC Line

10) Screw on cover of distlled water barrel.

8.4.3 Clean Waste Solution Barrel

Biological Hazard
Be sure to put on protective gloves, clothes, or even goggles if necessary. Discharge
waste solution according to local discharge standard and consult relative reagent
manufacturer and distributor.

1) To make sure the test switch is off.


2) Screw off waste solution pipe.
3) Pull out waste solution pipe
Press circle and pull out waste solution pipe.

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Figure 8­54 Hold Joint of Pipe


4) Screw off BNC signal line

Figure 8­55 Hold BNC Line Figure 8­56 Screw off BNC Line

Figure 8­57 Hold BNC Line


5) Screw off cover of waste solution barrel.
6) Pour waste solution out to the specified pool.
7) Clean inwall of waste solution barrel by distilled water, using a brush to clean if necessary.

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NOTE
Do not scratch the liquid level sensor when use a brush to clean.

8) Using a clean gauze to clean the external and cover of waste solution barrel.
9) Insert waste solution barrel.

Figure 8­58 Insert Pipe


10) Insert BNC signal line.
Insert BNC signal line to the joint and tighten.

Figure 8­59 Hold BNC Line Figure 8­60 Insert BNC Line

Figure 8­61 hold BNC Line


11) Screw on cover of waste solution barrel.
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8.4.4 Clean Detergent Barrel

1) To make sure the test switch is off.


2) To pull out detergent pipe.
Press circle and pull out detergent pipe.

Figure 8­62 Pull Out The Pipe

3) Screw off BNC signal line.

Figure 8­63 Before Screwing Figure 8­64 After Screwing

4) Screw off cover with liquid level sensor of detergent barrel.

Figure 8­65 Screw off Cover of Detergent Barrel Fiugre 8­66 Take out Cover with Liquid Level Sensor

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NOTE
Take out cover with liquid level sensor and put on clean desktop.

5) Pour the allowance of detergent into a clean container.


6) Clean the inwall of distilled water barrel by distilled water, using a brush to clean if necessary.

NOTE
Do not scratch the catheter and filter when using a brush to clean.

7) Clean liquid level sensor of detergent barrel by distilled water, using gauze to clean if necessary.
8) Using clean gauze to clean the external of detergent barrel.
9) Screw on cover with liquid level sensor of detergent barrel.
10) Connecting BNC signal line.
Insert the BNC signal line to the joint and tighten

Figu
re 8­67 Before Screwing Figure 8­68 After Screwing

11) Insert detergent pipe.

Figure 8­69 Insert Pipe


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8.4.5 Clean Drive Shaft of Aspirating Probe

1) To make sure the power switch is off.


2) Uplift the arm of aspirating probe to the highest point.
3) Using clean gauze to clean drive shaft
NOTE

Do not use alcohol or other corrosive detergent to clean drive shaft, otherwise will cause
stuck. Please use specified lubricant oil to maintain drive shaft.

8.4.6 Clean Drive Shaft of Stirrer

1) To make sure power switch is off.


2) Uplift the arm of stirrer to the highest point.
3) Using clean gauze to clean drive shaft of stirrer.
NOTE

Do not use alcohol or other corrosive detergent to clean drive shaft, otherwise will cause
stuck. Please use specified lubricant oil to maintain drive shaft.

8.4.7 Check Washing Hand

1) Click Maintenance Cuvette rinsing.


2) Check the rinse process to see whether the probe of group 1 to group 6 is parallel and in level or not, to
check whether probe tip of group 7 is parallel with the lower end surface of block or not.
3) To check whether there are stains or crack on probe tip or block. Clean or change probes if necessary.
NOTE

Do not use alcohol to clean washing hand, using the detergent provided by URIT to
clean will be better.

8.5 Maintenance for Every Three Months

8.5.1 Change Cuvette and Cuvette Bracket

It will appear scratch, stains on cuvette if used for a period. So it suggests replacing all cuvettes for every
three months to guarantee the accuracy of test result.

8.5.1.1 Detect Cuvette


1) Click Maintenance Cuvette Rinsing:

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Figure 8­70
2) Click Add water.

Figure 8­71
Click Cuv. Blank Reading

Figure 8­72

3) Observe the AD value

Figure 8­73
Check AD value of all cuvettes by clicking arrows of left and right.

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Figue 8­74
4) All AD values should be in the range of 40000 to 60000. If not, do the following step:
a) If the AD value exceeds 60000, adjust the potentionmeter according to 3.5.7.
b) If the AD value under the 40000, take out the cuvette which irradiate by light, observe the AD value, if it
is in the range, replace the cuvette; if there are no change or reduced, replacing light source. (refer to
8.6.3)
c) If the AD value of one row is too small, do the following steps:
To confirm the cuvette number which is problematic, the cuvette number corresponds with the number
on the left of list.
Screw off the fixed screws on the corresponding cuvette bracket.

Figure 8­75 Screw off the fixed screws on cuvette bracket


Take out cuvette bracket and observe if there are any problems on cuvette.

Figure 8­76 Take out cuvette


If there still have bubbles in cuvette, do step 1 to 4 again.
If there still have obvious stain on cuvette, clean cuvette by distlled water, if necessary, soak cuvette for ten
minutes by detergent then clean by distilled water again. If the cuvette still has stains, refer to 8.5.1.2 to
replace cuvette.
Refer to 8.5.1.2 to replace cuvette if there is fracture or scratch.

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8.5.1.2 Replacing Cuvette


Biological Hazard
Be sure to put on protective gloves, clothes, or even goggles if necessary.

1) Screw off fixed screws on cuvette bracket.


2) Take out bracket and cuvette.
3) Put new cuvette and bracket to incubation slot, fixed location hole to location pin on the reaction tray.
The two side of bracket should be in level with the near one.
NOTE
Do not touch the wall of cuvette to avoid stain on fingerprint.

Figure 8­77 Location hole Figure 8­78 Location pin

Figure 8­79 Two side in level with near one


4) Screwing on fixed screws and tighten.
5) Do step 2 to 4 repeatly.

8.5.2 Clean Distilled Water Tank and Negative Pressure Tank for Draining Waste Solution
1) To make sure power switch is off.
2) Open rear baffle.
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3) Empty water in distilled water tank.


4) Exhaust negative pressure in the tank which used for draining waste solution.
5) Screw off distilled water tank and negative pressure tank by hands or special tool.
6) Clean tank 3 to 4 times by water and dry inner by clean cloth.
7) Reinstall relative components.

Figure 8­80

8.6 Non‐Periodical Maintenance

8.6.1 Clear/Replace Aspirating Probe

This chapter mainly introduced the methods when aspirating probe is blocking, staining etc.

CAUTION
Be careful to operate to avoid scratch finger.
Biological Hazard

Be sure to put on protective gloves, clothes, or even goggles if necessary.

1) To make sure the test switch is off.


2) Observe the position of aspirating probe over the washing pool.
3) Uplift the arm of aspirating probe to the highest point, then turn to a position where convinent for
operation.
4) Lightly pinch the block of the probe cover and take out the cover.

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Figure 8­81 Take out cover of aspirating probe

NOTE
Do not lose the spring and retaining screw when take out the cover of probe.

5) One hand gently hold the joint of aspirating probe, the other hand pinches the joint of liquid level pipe
and take out.

Figure 8­82 Take out plug of aspirating probe

NOTE
Pinch the joint of liquid level pipe with sand paper to increase friction force.

6) Using needle cleaner to clean inner of aspirating probe until it is unblocked.


7) Injecting water to aspirating probe from joint to inner by syringe until the dirt are all discharged.
8) If the probe still block after cleaing for several times, or there are scratch on inner and outer wall of
probe, replacing a new aspirating probe.
Put the new aspirating probe to location hole from up to down.
9) One hand gently holds the joint of aspirating probe, the other hand hold the Teflon tube, insert the probe
to the tube.
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Figure 8­83 The joint of probe completely insert to tube


10) Put on the cover of aspirating probe.
NOTE
1) Do not scatch the inner wall of probe when clean the probe by needle cleaner.
2) The tube must be plug tightly to avoid leakage.
3) If the front of tube is damage, cut a little if necessary.
4) Attention the spring can not hook up the aspirating probe, otherwise the anti­collision
will be caused. And the cover should fasten and sealing.

11) Switching on test switch.


12) Click Maintenance Probe and Stirrer Cleaning.
13) Observe the position of aspirating probe over washing pool; it should the same with the position which
observed in step 2.
If there exsits great deviation, do the above steps again. If the problem still exists, please contact URIT.

8.6.2 Replacing Stirrer


1) To make sure the power switch is off.
2) Preparing a new stirrer, using gauze or cotton swab with detergent to clean stirrer, then clean by gauze
with distilled water.
3) Uplift the arm of stirrer to the highest point, and then turn it to a position where convenient to operate.
4) Pull out cover of arm of stirrer from down to up.
5) Loosen two fixed screws and joint of stirring motor line.

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Figure 8­84

6) Take out stirrer.


7) Loosen two screws on the micromotor adjusting plate, then take out the plate.
8) Install the micromotor adjusting plate on the component of new stirrer.

Figure 8­85

9) According to 4.3 to install the stirring component on stirring arm.


NOTE
The position of micromotor adjusting plate should the same with origin.

8.6.3 Replacing Lamp


The service life of lamp is 1500 hours. Replacing the lamp if the lamp is damage or achieve rated life

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according to the following steps:


1) Turn off light and wait 15 to 20 minutes until it is cool.
2) The lamp component is located on the bottom of reaction tray.
3) Take out cuvette bracket, then take out cover of reaction tray. See figure 8­86

Figure 8­86

4) Loosen jackscrew and power joint of light, then take out lamp.
5) Connecting the lamp well and insert into lamp­socket.
6) Turn on power switch and install the cover of reaction tray.
7) Run the software, click RESET or Roate to Cuv.1, when the original position of cuvette is confirm, click
Maintenance Cuvette signal
8) Take out cover of reaction tray again and adjusting the position of lamp, then observe the A/D signal.
9) When the A/D signal value is biggest, tighten the jackscrew to fix lamp.
10) At last install the cover and other components of reaction tray.
NOTE
If the reaction tray does not move to original position, the A/D value could not be read.

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Figure 8­87 Lamp components

8.6.4 Solenoid Valve Rinse


It will cause water and air leakage if the solenoid valve is block. So it should better to clean valves irregularly.
1) Take out solenoid valve and push off circlip.
2) Loosen screw cap and take out piston rod.
3) Clean cavity and piston rod by distilled water.
4) Reinstall and test after power on.

Figure 8­88

8.6.5 Check/Replacing BNC Line


1) To make sure the test switch is off.
2) Check whether there is rupture, wire expose and damage or not, etc. If not, it is not need to replacing
BNC line, if so, do the following steps:
3) Screw off BNC line and replacing a new one.

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NOTE
Attention one to one correspondence when screw off the BNC line to avoid wrongly
insert.

4) To connect BNC line.


Insert the line and fixed well.

8.6.6 Replacing Waste Solution Tube


The waste solution tube will appear block after long time use. It is suggest to replacing the specified tube.
There are two specification tubes for URIT­8021A.

8.7 Long­time Disuse Maintenance

If the instrument will not use for a long time, do the following steps before power off.
1) Click Maintenance Cuvette Rinsing.

Figure 8­89
Click WASH to rinse all cuvettes.

Figure 8­90

2) Repeat step 1 at least two times.


3) Pull out distilled water tube.
Press circle and pull out tube.
NOTE
Put the tube on a clean desk after pulling out.

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Figure 8­91 Pull out tube


4) Click Maintenance Cuvette Signal;

Figure 8­92
Click Water Probe and Water Arm repeatly until there are no waste solution discharge from waste solution
tube.

Figure 8­93

5) Empty distilled water barrel, detergent barrel and waste solution tube.

Biological Hazard
Be sure to put on protective gloves, clothes, or even goggles if necessary.

According to the local discharge standard to dispose waste solution and consult reagent
manufacturer or distributor.

6) Turn off test switch, main power switch and pull out plug.
7) Close the up cover.
8) Put on a dust cover.

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9 TROUBLESHOOTING
This chapter lists the various malfunctions, along with probable causes and recommended remedies to
correct the problem quickly and easily. If the problem still exists after following the recommended remedy,
contact URIT for Technical support.
CAUTION
Handle malfunction with utmost care and confirm if it is necessary to cut off the power supply at the
first.

Biological Hazard
Put on protective gloves to avoid contacting chemical solution. If it spill to human body, wash it off
with water immediately.

9.1 Troubleshooting Guide

To eliminate malfunction easily and correctly, users should read through the Operating Manual and be
familiar with the routine operation and maintenance of URIT­8021A .In general, there are three steps to
handle with malfunction:
Ø Step 1: Confirming Malfunction
Users should not only confirm the malfunction, but also clearly know what the normal status should be when
the malfunction is eliminated.
Ø Step 2: Classifying Malfunction
Malfunction can be categorized into three types.
l Malfunction relating to hardware
l Malfunction relating to software
l Malfunction relating to operation and analyses.
If malfunction relates to the hardware or software, contact your local distributor or URIT. If malfunction
relates to the operation and analyses, refer to the troubleshooting table below for solution.
Ø Step 3: Eliminating Malfunction
The maintenance engineer authorized by URIT takes proper measures to correct the problem.
Users can also eliminate the malfunction under the directions of maintenance engineer.

9.2 Obtaining Technical Help

Our Customer Service Office is available to help if the problem is beyond the scope of this manual or if you
need more technical assistance from URIT. Before calling, please identity the following information:
1) The instrument’s model;
2) The serial number of instrument;
3) The specific symptom and operating condition;
4) The data and report relating to the problem.
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9.3 Troubleshooting Method

The troubleshooting table below presents the various problems and malfunctions that may occur during
operation. If the problem can not be solved through the recommended methods, contact URIT please.
NOTE
For replacement parts of the instrument, refer to Appendix 1.

Table 9­1 Troubleshooting


SN SYPTOM POSSIBLE CAUSE REMEDY
1) Connect power cord correctly.
1) Incorrect connection with
2) Check if the power receptacle is in good
power cord.
condition.
2) No electricity with power
3) Replace fuse(8A)
Instrument is not receptacle.
4)Confirm select the correct connector at
active when power 3) The safety fuse is fusing
1 [System parameter—instrument
is on. 4) Improper COM interface
parameter—COM set]
is selected.
5) Make sure the RS232 communication cable
5) Communication cable
is connected to PC correctly.
error.
If the problem still persists, contact your local
distributor or URIT.
1) Select [Maintenance—Cuvette rinse],Check
if reaction cuvettes are dirty or damage.
Cuvette dirty or damage Replace them if necessary
2 Cuvette blank error
Light source aging 2) Replace light source.
3) If the problem persists, contact your local
distributor or URIT.
1) Check or replace the lamp holder
1) Bad contact of lamp
2) Replace the lamp.
3 Lamp is dark holder.
3) If the problem persists, contact your local
2) Lamp is burned out.
distributor or URIT.
1) Check flow path tubes. Reconnect or
1) Air leaks in the flow replace tubes if necessary.
paths. 2) Reconnect meter regulator. Eject air
Inaccurate
2) Air bubbles are formed in bubbles.
aspirated volume of
4 meter regulator. 3) Unclog or replace probe.
reagent or sample.
3) Probe is clogged. 4) Check magnetic valve and replace it if
4) Magnetic valve problem. necessary.
5) If the problem persists, contact your local
distributor or URIT.

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Water or detergent
1) Flow path tube is leaky.
does not come out 1) Reconnect the tube or replace it.
2) Flow path tube is
through the probe 2) Unclog the tube.
clogged.
5 washing pool or 3) Replenish water or wash solution.
3) Water or wash solution is
stirrer washing 4) If the problem persists, contact your local
used up.
pool. distributor or URIT.

1) Reconnect the tube or replace it.


1) Flow path tube is leaky. 2) Unclog the tube.
2) Flow path tube is 3) Check the vacuum pump and replace it if
Adding or draining
6 clogged. necessary.
water is abnormal
3) Vacuum pump error. 4) If the problem persists, contact your local
distributor or URIT.

A certain movable 1) Check the wire of light coupling or replace


Light coupling is short
part is out of the light coupling.
7 circuited or broken.
control. 2) If the problem persists, contact your local
distributor or URIT.
1) Liquid level sensor board 1) Check the liquid level sensor board and
Liquid level sensor is defective. replace it if necessary.
8 is out of order 2) Bad contact with liquid 2) Reconnect the liquid level sensor board.
level sensor board. 3) If the problem persists, contact your local
distributor or URIT.
1) Select Maintenance –­­ Cuvette Rinsing to
1) Cuvette is dirty or check whether cuvette is dirty. Clean or
breakage. replace the cuvette.
2) Inaccurate aspirated 2) Check sample injector and tube whether
volume of reagent or leakage existed.
Inaccurate test
sample. 3) Replace the lamp.
result or poor
3) Lamp is deteriorated. 4) Set the parameter follows the operation
9 repeatability.
4) Parameters of analytical manual. Make sure the instrument is well
item are set improperly. grounded by means of the ground pole.
5) Ground wire is absent 5) Check if the reagent is certified. Perform
with power supply. recalibration.
6) Reagent problem. 6) If the problem persists, contact your local
distributor or URIT.

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1) Turn the power switch off, slowly rotate the


problem parts and observe if there is abnormal
1) Communication error. noisy or if it is stuck.
2) Mechanical parts are 2) Enter into system parameter interface,
loose or stuck. select Engineer set to adjust parameter. (Only
Abnormal motor 3) Light coupling joint of accessible to professional person authorized
10
movement motor is loose. by URIT).
4) Light coupling is 3) Check the light coupling and replace it if
defective. necessary.
4) If the problem persists, contact your local
distributor or URIT.

1) Replace the stirrer motor.


Stirrer does not 1) Stirrer motor is broken. 2) Reinstall the stirrer motor.
11 work. 2) Bad contact of stirrer 3) If the problem persists, contact your local
circuit. distributor or URIT.

1) Check the flow path tube. Reconnect or


replace the tube if necessary.
1) Flow path tube is leaky. 2) Check the magnetic valve and replace it if
Water leaks from
2) Magnetic valve problem. necessary.
12 nozzles of cuvette
3) Vacuum pump problem. 3) Check the vacuum pump and replace it if
cleaner.
necessary.
4) If the problem persists, contact your local
distributor or URIT.
1) Check the flow path tube. Reconnect or
replace the tube if necessary.
2) Check the magnetic valve and replace it if
1) Flow path tube is leaky.
necessary.
Water drops adhere 2) Magnetic valve problem.
13 3) Check the exterior of probe. Clean or
to the tip of probe. 3) The exterior of probe is
replace the probe if necessary.
dirty or scathed.
4) If the problem persists, contact your local
distributor or URIT.

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1) Check if the reagent tray is sealed


completely.
2) Check if the heat dissipation device works
The cooling system is failed normally.
Reagent tray
or cooling temperature is 3) Check if the refrigerants are used up.
14 cannot be cooled.
not low enough. 4) Check if the circulation system of the
cooling device works normally.
5) Replace the peltier.
6) If the problem persists, contact your local
distributor or URIT.
1) Enter into system parameter interface,
select Engineer set to adjust parameter. (Only
accessible to professional person authorized
1) Instrument parameters
by URIT).
are set improperly.
2) Read through the Operating Manual and
2) Human carelessness,
Probe and stirrer avoid human mistake.
such as reagent bottle lid is
conflict with each 3) DO NOT put foreign objects on the
15 not opened.
other. operation panel.
3) Put foreign objects on the
4) Check if the motor is installed and works
operation panel.
properly.
4) Motor problem.
5) Check the light coupling and replace it if
5) Light coupling error.
necessary.
6) If the problem persists, contact your local
distributor or URIT.

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URIT‐8021A Service Manual

CHAPTER 10 Measurement Method


URIT­8021A automatic chemistry analyzer is based on the Lambert’s Beer principle to analyse and
calculate. This chapter will mainly introduce the calculation method.

10.1 Method of Measurement


The URIT­8021A performs measurement with the following methods:
1) Endpoint method
2) Two­point end method
3) Two­point rate method
4) Rate method(Kinetic method)

10.2 Endpoint
In the endpoint measurement, the reaction reaches equilibrium after a period time, it can be considerate that
all analytes(substrates) have changed into products. It’s best that the absorbance is directly proportion to the
analytes’ concentration.
Endpoint method (single reagent) reaction graph see as figure 10­1:

Figure 10­1 Single Reagent Endpoint Method Graph

R1: Add first reagent


S: Add sample
a: Test start point of absorbance
b: Test end point of absorbance
1 5: Test point of reagent­blank absorbance
The URIT­8021A system records the absorbance change start from adding R1 and end at the maximum
reaction time.
n Setup of reagent parameter:
From S to a: The first incubate time of first reagent
From a to b: The number of test points
n Result calculation:

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URIT‐8021A Service Manual

C =(A1 ­ A0)´ K
C: The concentration of reactant
K: Calculation factor

A1: The average of absorbance from a to b

A0: The average of absorbance from the first test point to the fifth test point before adding sample.

(R1 blank)
NOTE
When double reagents test use endpoint method, the result calculation is the same as the above
calculation formula that test point absorbance substracts R1 blank, then multiplied by K to get the
concentration.

10.3 Two‐Point End Method (Double Reagent Endpoint)


Two­point end method add sample blank and volume correction factor to calculate, maily used in the double
reagent endpoint. See figure 10­2:

Figure 10­2 Double Reagent Two­Point End Method Graph


R1: Add first reagent
S: Add sample
R2: Add second reagent
A: Test start point of absorbance
B: Test end point of absorbance
c d: Special blank test start point, in general to take five test points. System will take
the blank test points automatically according to the time, if the set time is insufficient
five test points
1 5: Test point of reagent­blank absorbance
The URIT­8021A system records the absorbance change start from adding R1 and end at the maximum
reaction time.
n Setup of reagent parameter:
From S to R2: The incubate time of first reagent
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URIT‐8021A Service Manual

From R2 to a: The incubate time of second reagent


From a to b: The number of test point
n Result calculation:

C =(
[ A2 ­ A0)­ ( A1 - A0 ) ´ k1 ] ´ K
C: The concentration of reactant
K: Calculation factor

K1: Volume correction factor

A1: The average of absorbance from c to d. (Sample blank)

A2: The average of absorbance from a to b

A0: The average of absorbance from the first test point to the fifth test point before adding sample.

(R1 blank)

VR1 + VS
k1 =
VR1 + VS + VR 2
VR1: The volume of first reagent
VRS: The volume of sample
VR2: The volume of second reagent

10.3 Two‐Point Rate Method (Fixed‐Time)


Two­point rate method (Fixed ­Time), namely, first­order kinetic method, the reaction velocity is directly
proportional to the first­order of substrate concentration within a special period. As the substrate is
consumed continuously, the velocity is descreasing gradually, and the absorbance is nonlinear increasing
descreasing). See the graph as 10­3:
Two­point rate method allows the check of substrate depletion. When detecting substrate depletion, the
system will perform the related operation and flag the test result.

Figure 10­3 Two­Point Rate Method Graph (Single reagent, positive direction reaction)
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URIT‐8021A Service Manual

R1: Add first reagent


S: Add sample
a: Test start point of absorbance
b: Test end point of absorbance
The URIT­8021A system records the absorbance change start from adding R1 and end at the maximum
reaction time.
n Setup of reagent parameter:
From S to a: The incubate time of first reagent
From a to b: The number of test point
n Result calculation:

æ A - Aa ö
C = çç b ÷÷ ´ K
è tb - tb ø
C: The concentration of reactant
K: Calculation factor

Aa: The absorbance of point a

Ab: The absorbance of point b

ta: The time of point a

tb: The time of point b


NOTE
1) tb­ta is the time that the number of test points multiplied by the period time.
2) The double reagent calculation is the same as single reagent.

10.4 Rate Method (Kinetic method)


Rate method (kinetic method) namely zero­order kinetic method, the reaction velocity is not related to
substrate concentration. The analytes absorbance descreasing or inscreasing evenly at a given wavelength,
and the change rate is directly proportional to the activity of the analytes. The kinetic method is usually used
to measure enzyme activity. See graph 10­4.
In fact, it’s impossible for the substrate concentration to be absolutely high, and the reaction will be no longer
a zero­order reaction when the substrate is consumed to certain degree. Therefore, the substrate depletion
appears in the rate method. In addition, the reaction can become steady only after a period of time, because
the reaction is complicated at the beginning and there are miscellaneous reactions due to complex serum
compositions. Please set the reagent parameter according to the reagent manual that provided by reagent
manufacturer.

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URIT‐8021A Service Manual

Figure 10­4 Rate Method Graph(Single reagent, negative direction reaction)


The URIT­8021A system records the absorbance change start from adding R1 and end at the maximum
reaction time.
n Setup of reagent parameter:
From S to a: The incubator time of first reagent
From a to b: The number of test point
n Result calculation:

C = DAab / min´ K
C: The concentration of reactant
K: Calculation factor

DAab / min : The absorbance change rate from a to b.

NOTE
The double reagent calculation is the same as single reagent.

10.5 Substrate Exhaust


In rate method and two­point rate method appears substrate exhaust because the concentration of part of
sample is high. URIT­8021A provides two methods to check of substrate exhaust, can be choose two or one.
But two­point rate method only chooses the method of first substrate exhaust. The graph 10­5 is substrate
exhaust in the single reagent negative direction reaction.

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URIT‐8021A Service Manual

Figure 10­5

10.5.1 Substrate Exhaust Method 1(Substrate exhaust Limit)

Make experiment to obtain the absorbance of substrate exhaust and set the limit. The average value of the
last three points in positive reaction is greater than the absorbance value of substrate exhaust limit. If the
average value smaller than the absorbance value in negative reaction, it could judge as substrate exhaust.
The red box in the figure 10­6 is the substrate exhaust setup.

Figure 10­6

Figure 10­7 Substrate exhaust (Single reagent, positive direction)

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URIT‐8021A Service Manual

10.5.2 Substrate Exhaust Method 2(Slope Ratio)

The second method of substrate exhaust is using the slope ratio of reaction curve (absorbance change rate)
to judge.

The meaning of each parameter:


First slope (First read point section): The slope in the first read point section that setted in the reagent
parameter.
Second slope (Second read point section): The slope in the second read point section that setted in the
substrate exhaust method 2.
Begin point: The begin read point of second slope ratio. It should be not greater than the begin read point of
first slope ratio. The begin point of single reagent is the point that start to add sample, and the double
reagents is the point that start to add second reagent
End point: The end read point of second slope ratio that less than the end read point of first slope.
Slope ratio: First slope/ second slope
When the calculated slope ratio is less than the set value, the system will judge for substrate exhaust. This
method can be used simultaneously with the method 1 or used alone. But two­point rate method only use
method 1.
As long as choosing the substrate exhaust method 2, system will automatically take the second slope as the
calculated slope when the instrument judge for substrate exhaust. The result of the sample is: second
slope K.
Caution
The begin point of second slope is restricted by begin point of first slope. Begin point should be not
greater than the begin point of first slope, and end point not greater than the end point of first
slope.

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URIT‐8021A Service Manual

Figure 10­8
NOTE
The judgement method of double reagent is the same as the method in 10.4.2.

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Appendix A URIT‐8021A Circuit Schematic Diagram

8021A Circuit
Diagram.pdf

143

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