Name: Missy Arabella C.

Pame Date Performed: June 15, 2021

Course and Year: BSN1-C Name of Lab Instructor: Ma’am Guadalupe Oracion

COLLOIDS
Activity No. 2

I. OBJECTIVES:
1. To study some properties of colloids.
II. APPARATUS: Test tubes, 2 dialysis tubes, 2 beakers (250 ml), funnel
III. MATERIALS: 10 ml ferric hydroxide colloid, 10 ml arsenous sulfide colloid, 4 ml potassium
chromate solution, 2 ml each of 0.1N sodium chloride, 0.1N calcium chloride,
0.1N chromic chloride solution, a piece (1/2 x ½ inches) of gelatin, apiece (6
x 3 cellophane), filter paper.
IV. PROCEDURE:

1. DIALYSIS
a) Obtain 2 dialysis tubes and 2 pieces of dialysis membrane (cellophane) from the counter.
Cover the lipped end of each of the dialysis tube with a membrane and fasten it securely with
a piece of thread or rubber band.

b) Prepare 2 small beakers each half-filled with water.

c) Hold one of the dialysis tube in one beaker using the iron clamp with the iron stand. Make
sure that the membrane just submerge beneath the surface of the water. Pour into the
dialysis tube about 4 ml of colloidal Fe(OH)3.

d) Repeat (c) in the other beaker but use 4 ml of K2CrO4 solution instead of Fe(OH)3 colloid.

e) Set the beakers containing the dialysis tubes aside for about 20 minutes and record the color
change of the solution in the beaker.

2. Filter 2 ml of Fe(OH)3 colloid. Can a colloid be separated by the use of filter paper?

3. Boil 10 ml of water in a hard glass tube. Dissolve about ½ gm of gelatin in the boiling water. Allow
the tube to cool somewhat; then cool it rapidly by holding it under a stream of tap water. Set the
tube aside for later observations. What is a gel?

4. a.) Take 10 ml of distilled water in a test tube and add 1 ml of coconut oil. Shake vigorously. Put aside
in your table for later observation.
 b.) Again take 10 ml of distilled water in a test tube. Add 10 drops of concentrated soap solution.
Now add 1 ml of coconut oil. Shake vigorously for about 10 seconds. Note any difference in the
results obtained in the foregoing operation and this one. Explain what is emulsion? What is surface
tension?

5. Precipitation of Electrolytes
Take about 6 ml of the As2S3 colloid. Divide into 3 equal parts.
a) To one add 0.1 N NaCl solution drop by drop until coagulation occurs. Record the number of
drops needed.
b) To a second add 0.1 N CaCl2 solution until coagulation occurs. Record the number of drops
needed.
c) To the third add 0.1 N CrCl3 solution until coagulation occurs. Record the number of drops
needed. Explain the difference in the effect of the various electrolytes.

6. Protective Action of Colloid

Take about 4 ml of the Fe (OH)3 colloid. Divide into 3 equal parts.
a) To one add 2 ml of As2S3 colloid. Observe.
b) To the other add an equal volume of the gelatin suspension saved from (3) and shake
vigorously. Add 2 ml of the As2S3 colloid. Compare the results with that of 6a. Explain the
function of the gelatin.
RESULTS AND DISCUSSIONS:
Answer:
In this procedure, Setup A which is the ferric hydroxide after 20 minutes I observed that only a small
amount of color was in the water and in my opinion ferric hydroxide take more time to completely
change the color the water. In setup B the potassium chromate which was placed in the water for 20
minutes changed the color of the water completely and it only take 20 minutes so I could say that
setup A takes more time to change the color that setup B. A colloid cannot be separated with ordinary
filtration especially if one would use a filter paper to separate it because the colloid solution is smaller
in suspension. In this procedure I observe that when a gelatin is placed in a boiling water and is being
shaken vigorously the gelatin would mi in the boiling water and when it is cooled down using a tap
water and is being set aside for a few minutes, upon observation when the solution is cooled down
it created a gel like texture. The texture is ranging from either a soft or weak and hard or tough
solutions. For the first part of the fourth procedure the adding of coconut oil into the 10 ml distilled
water, after shaken vigorously and is set aside, I could say that the solution created a surface tension
between the solutions because water is holding of the coconut oil solution. On the second part of
the procedure when we add dishwashing soap to the first two solution the coconut oil and water,
the dishwashing soap would act as an emulsifier to mix the solution that would repel each other; the
coconut oil and distilled water. For the first part of the fifth procedure, when you add 0.1 N NaCl
solution into 6 ml arsenic sulfide (As2S3), there was no significant change when it comes to the color
or texture, it’s as if no other solution was added into the tube of arsenic sulfide. On the second part
of the procedure 0.1 N CaCl2 was added into the tube with arsenic sulfide solution and when the two
solutions were mixed both reacted resulting to the changing of it color and texture, the color was
white and for the texture it was like a sticky like texture. On the third part of the procedure 0.1 N
CrCl3 was added into the tube with arsenic sulfide solution and when the two solutions were mixed
 both reacted resulting to the changing of two colors, on the upper half part is dark green and the
lower half part is light green. Between the three parts I observe that the first part of the procedure
has no change because it a controlled setup. On the second part of the procedure there was a slight
change, when you add two ml of arsenic solution into the tube with ferric hydroxide colloid, the
result was on the lower part of the solution there was a black substance on the middle part the color
is light pink and the upper part a dark pink color. On the third part of the procedure when you add a
gelatin and arsenic sulfide into the tube of ferric hydroxide it resulted in to a changing of color and
texture. The color was pink with a tinge of orange and there was a blackish to brownish substance
on the lower part of the solution and for the texture it was a sticky or gooey like. The function of
gelatin in the mixture is to work as a hemostatic to provide a physical framework in the solution.
APPLICATION:
1. What is a colloid?
Answer: Colloid is mixture which is made up of small particles dispersed throughout another
substance. Any substance consisting of particles substantially larger than atoms or ordinary molecules
but too small to be visible to the unaided eye; more broadly, any substance, including thin films and
fibres, having at least one dimension in this general size range.
2. What is the importance of colloidal property in biochemistry?
Answer: The importance of colloidal property is that it helps the solution to remain dispersed and not
settle into the bottom of the container. It also prevents the separation of particle in ordinary filtration.
REFERENCES:
Britannica, colloid. Retrieved from: https://www.britannica.com/science/colloid
Soult, A. (2019) colloids and suspensions. Retrieved from:
https://chem.libretexts.org/Bookshelves/Introductory_Chemistry/Book%3A_Chemistry_for_Allied_He
alth_(Soult)/07%3A_Solids_Liquids_and_Gases/7.06%3A_Colloids_and_Suspensions
Totre, J., Ickowicz, D., Domb, A.J. (2011) properties of hemostatic application of gelatin. Retrieved
from:
https://www.researchgate.net/publication/230208126_Properties_and_Hemostatic_Application_of_G
elatin