Exportlist

TY - CONF
AB - The proceedings contain 2 papers. The topics discussed include: carbonaceous

nanoparticles and their interactions with biological cells; dose-dependent
intracellular reactive oxygen and nitrogen species production from particulate
matter exposure: results from ambient samples and chamber experiments; biofuel and
reference diesel particles: differences in inflammatory and oxidative effects; real
life PM emissions from traffic and human exposure implications; disinfection of
water using silver and copper nanoparticle impregnated activated carbon; amorphous
silicon dioxide nanoparticle interactions with pulmonary epithelial cells with and
without a pre-existing protein corona; physiochemical properties of nanoparticles
determine their in vitro cytotoxicity; potential impact of sublethal levels of
nanomaterials on interactive behavior of environmental bacteria; effect of size and
charge of metal ions on hydrogen peroxide stability in silica hydrogels; UV-
assisted synthesis of carbon nanotube-TiO2 nanocomposites for enhanced
photocatalytic air purification; effect of thermal treatment on the characteristics
of PES/PVA nanocomposite membranes modified with TiO2 nanoparticles: a comparitive
study between 1-step and 2-step thermal treatment; and eco-friendly dyeing of
electrospun cellulose nanofibers with reactive dye using ultrasonic energy.
DB - Scopus
N1 - Export Date: 28 August 2023; Cited By: 0
PY - 2016
ST - Environmental Aspects, Applications and Implications of Nanomaterials and
Nanotechnology 2016 - Topical Conference at the 2016 AIChE Annual Meeting
T2 - Environmental Aspects, Applications and Implications of Nanomaterials and
TI - Environmental Aspects, Applications and Implications of Nanomaterials and
UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-
85019046999&partnerID=40&md5=db7be0158733a68aa7d6cb78ce0afbb0
ID - 5569
ER -
TY - JOUR
AB - Declaration of Competing Interest statements were not included in the
published version of the following articles that appeared in previous issues of
Biocatalysis and Agricultural Biotechnology. The appropriate Declaration/Competing
Interest statements, provided by the Authors, are included below. 1.
“Biodegradation of triphenylmethane dye malachite green by a newly isolated fungus
strain” [Biocatalysis and Agricultural Biotechnology, 2019; 17C; 672–679]
https://10.1016/j.bcab.2019.01.030 Declaration of competing interest: The authors
declare that they have no known competing financial interests or personal
relationships that could have appeared to influence the work reported in this
paper.2. “Microbial lipases: An overview of screening, production and purification”
[Biocatalysis and Agricultural Biotechnology, 2019; 22C: 101368] DOI-
https://10.1016/j.bcab.2019.101368 Declaration of competing interest: The authors
paper.3. “Addition of electron shuttling compounds and different pH conditions for
hydrogen production by a heat-treated sludge” [Biocatalysis and Agricultural
Biotechnology, 2020; 23C: 101507] DOI – https://10.1016/j.bcab.2020.101507
Declaration of competing interest: The authors declare that they have no known
competing financial interests or personal relationships that could have appeared to
influence the work reported in this paper.4. “Enzymatic esterification for the
synthesis of butyl stearate and ethyl stearate” [Biocatalysis and Agricultural
Biotechnology, 2018; 16C: 373–377] DOI – https://10.1016/j.bcab.2018.09.008
influence the work reported in this paper.5. “Enzyme-catalyzed production of
emollient cetostearyl stearate using different immobilized commercial lipases under
vacuum system” [Biocatalysis and Agricultural Biotechnology, 2018; 15C: 229–234]
DOI – https://10.1016/j.bcab.2018.06.012 Declaration of competing interest: The
authors declare that they have no known competing financial interests or personal
paper.6. “Production, Characterization and Emulsifying property of
exopolysaccharide produced by marine isolate of Pseudomonas fluorescens”
[Biocatalysis and Agricultural Biotechnology,2018; 16C: 320–325] DOI –
https://10.1016/j.bcab.2018.08.023 Declaration of competing interest: The authors
paper.7. “Isolation, screening and characterization of plant growth promoting
rhizobacteria from rhizospheric soils of selected pulses” [Biocatalysis and
Agricultural Biotechnology, 2020; 27C: 101685] DOI –
paper.8. “Anti-inflammatory activity of a serine protease produced from bacillus
pumilus SG2” [Biocatalysis and Agricultural Biotechnology, 2019; 17C: 538–544] DOI
– https://10.1016/j.bcab.2019.01.015 Declaration of competing interest: The authors
paper.9. “Highly efficient fungal pectinase and laccase producers among isolates
from flax retting liquor” [Biocatalysis and Agricultural Biotechnology, 2020; 25C:
101570] https://10.1016/j.bcab.2020.101570 Declaration of competing interest: The
paper.10. “Spectral characterization of bioactive compounds from microalgae: N.
Oculata and C. Vulgaris” [Biocatalysis and Agricultural Biotechnology, 2019; 19C:
101094] DOI – https://10.1016/j.bcab.2019.101094Declaration of competing interest:
The authors declare that they have no known competing financial interests or
personal relationships that could have appeared to influence the work reported in
this paper.11. “In silico molecular docking of astaxanthin and sorafenib with
different apoptotic proteins involved in hepatocelllar carcinoma” [Biocatalysis and
Agricultural Biotechnology, 2019; 19C: 101076] https://10.1016/j.bcab.2019.101076
influence the work reported in this paper.12. “Studies on Estrone Biodegradation
Potential of Cyanobacterial Species” [Biocatalysis and Agricultural Biotechnology,
2019; 17C: 576–582] https://10.1016/j.bcab.2019.01.022 Declaration of competing
interest: The authors declare that they have no known competing financial interests
or personal relationships that could have appeared to influence the work reported
in this paper.13. “Hesperidin inhibits cell proliferation and induces
mitochondrial-mediated apoptosis in human lung cancer cells through down regulation
of β-catenin/c-myc” [Biocatalysis and Agricultural Biotechnology, 2019; 18C:
101065] https://10.1016/j.bcab.2019.101065 Declaration of competing interest: The
paper.14. “One-pot green route synthesis of silver nanoparticles from jack fruit
seeds and their antibacterial activities with escherichia coli and salmonella
bacteria” [Biocatalysis and Agricultural Biotechnology, 2019; 20C: 101241] DOI-
paper.15. “Persister cell development among Enterobacteriaceae, Pseudomonadaceae,
Mycobacteriaceae and Staphylococcaceae biotypes: A review” [Biocatalysis and
Agricultural Biotechnology, 2019; 22C: 101401] https://10.1016/j.bcab.2019.101401
influence the work reported in this paper. © 2020
C7 - 101872
DB - Scopus
DO - 10.1016/j.bcab.2020.101872
M3 - Erratum
PY - 2021
ST - Erratum regarding missing Declaration of Competing Interest statements in
previously published articles (Biocatalysis and Agricultural Biotechnology (2019)
20, (S1878818119307182), (10.1016/j.bcab.2019.101241))
T2 - Biocatalysis and Agricultural Biotechnology
TI - Erratum regarding missing Declaration of Competing Interest statements in
previously published articles (Biocatalysis and Agricultural Biotechnology (2019)
20, (S1878818119307182), (10.1016/j.bcab.2019.101241))
85096511049&doi=10.1016%2fj.bcab.2020.101872&partnerID=40&md5=aab2a9a9fff9ed812514c
1826d196b72
VL - 36
ID - 5198
ER -
TY - JOUR
AB - The unlimited use of nanoparticles (NPs) results in toxic impacts on
different tissues. The current study aimed to compare the adverse effects of AgNPs
and TiO(2)NPs on the parotid gland of adult male albino rats as regards the
histopathological, immunohistochemical, and biochemical changes, exploring the
possible underlying mechanisms and the degree of improvement after cessation of
administration. Fifty-four adult male albino rats were divided into control group
(I), AgNPs-injected group (II), and TiO(2)NPs-injected group (III). We measured the
levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL-6) in the
serum, and levels of MDA and GSH in parotid tissue homogenate. Quantitative real-
time polymerase-chain reaction (qRT-PCR) was used to measure the expression levels
of peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1-
alpha), nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), mouse double
minute 2 (MDM2), Caspase-3 Col1a1, and Occludin. Parotid tissue sections were
examined by light microscope (Hematoxylin & Eosin and Mallory trichrome stains),
electron microscope, and immunohistochemical examination of CD68 and anti-caspase-3
antibodies. Both NPs severely affected the acinar cells and damaged the tight
junction between them by enhancing expression of the inflammatory cytokines,
inducing oxidative stress, and disturbing the expression levels of the studied
genes. They also stimulated fibrosis, acinar cell apoptosis, and inflammatory cells
infiltration in parotid tissue. TiO(2)NPs effects were less severe than AgNPs.
Cessation of exposure to both NPs, ameliorated the biochemical and structural
findings with more improvement in TiO(2)NPs withdrawal. In conclusion: AgNPs and
TiO(2)NPs adversely affected the parotid gland, but TiO(2)NPs were less toxic than
AgNPs.
AN - WOS:000979875400001
AU - Aal, S. M. A.
AU - Mohammed, M. Z.
AU - Abdelrahman, A. A.
AU - Samy, W.
AU - Abdelaal, G. M. M.
AU - Deraz, R. H.
AU - Abdelrahman, S. A.
C6 - MAY 2023
DA - JUL 4
DO - 10.1080/01913123.2023.2205924
IS - 4
PY - 2023
SN - 0191-3123
1521-0758
SP - 339-363
ST - Histological and biochemical evaluation of the effects of silver
nanoparticles (AgNps) versus titanium dioxide nanoparticles (TiO(2)NPs) on rat
parotid gland
T2 - ULTRASTRUCTURAL PATHOLOGY
TI - Histological and biochemical evaluation of the effects of silver
nanoparticles (AgNps) versus titanium dioxide nanoparticles (TiO(2)NPs) on rat
parotid gland
VL - 47
ID - 6161
ER -
TY - JOUR
AB - Aim: The present study was carried out to determine the effects of ZnO
nanoparticles (ZnO-NPs) on intestinal function and pathophysiological alteration.
Materials & methods: ZnO-NPs were synthesized and their characterizations were
performed using various techniques. The Wistar male rats fed with normal diet
and/or high fat diet (HFD) for 8 weeks and then orally received ZnO-NPs (5, 50 and
100 mg/kg bodyweight) for 28 days. The oxidative stress (SOD, CAT, GPx),
inflammatory (TNF-alpha, iNOS) and apoptosis pathways (Bcl2, Bax and p53) genes
expression and protein levels were measured by real-time polymerase chain reaction
and available kit, respectively. The activity of Caspase-3, antioxidant capacity,
as well as inflammatory markers were determined. The histological alterations of
the large and small intestine were also evaluated with haematoxylin and eosin (H&E)
as well as TdT dUTP nick end labeling (TUNEL) assay. The biochemical factors,
viability and antioxidant activity were also determined in Caco-2 cells. Results:
It was found that the antioxidant enzymes activity and genes expression markedly
increased, while inflammatory and apoptosis pathways and TNF-alpha levels
significantly decreased in the intestine of HFD-fed rats treated with 5 mg/kg ZnO-
NPs. Intestinal morphological changes were also restored by 5 mg/kg ZnO-NPs in HFD
group. Conclusion: Treatment of rats with 50 and 100 mg/kg ZnO-NPs significantly
induced intestinal injury, while treatment with 5 mg/kg ZnO nanoparticle normalized
intestinal functions and structure. This study showed the synergistic effects of
ZnO-NPs and HFD administration on liver enzyme, oxidative stress, apoptosis,
inflammation, morphological changes and cell toxicity.
AN - WOS:000452396700008
AU - Abbasi-Oshaghi, E.
AU - Mirzaei, F.
AU - Mirzaei, A.
DA - NOV
DO - 10.2217/nnm-2018-0202
IS - 21
PY - 2018
SN - 1743-5889
1748-6963
SP - 2791-2816
ST - Effects of ZnO nanoparticles on intestinal function and structure in
normal/high fat diet-fed rats and Caco-2 cells
T2 - NANOMEDICINE
TI - Effects of ZnO nanoparticles on intestinal function and structure in
normal/high fat diet-fed rats and Caco-2 cells
VL - 13
ID - 6363
ER -
TY - JOUR
AB - Purpose: This study evaluated the effects of titanium dioxide nanoparticles
(TiO2 NPs) on liver and intestine of normal rats. Methods: Male rats were divided
into four groups as follows: 1) control rats, 2) control rats that orally received
10 mg/kg TiO2 NPs, 3) control rats that orally received 50 mg/kg TiO2 NPs, and 4)
control rats that orally received 100 mg/kg TiO2 NPs. After 30 days, the NLRP3
inflammasome pathway (NLRP3, caspase-1, and IL-1 beta), antioxidant pathway
(superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT]),
inflammatory pathway (inducible nitric oxide synthase [iNOS] and tumor necrosis
factor-alpha [TNF-alpha]), and the apoptosis pathway (p53, Bax, Bcl-2, and caspase-
3) were determined in the intestine and liver of the rats. H&E and Masson's
trichrome (MT) staining as well as TUNEL assay were used to examine the liver and
the intestine. Biochemical factors, cytotoxicity, ROS generation, and apoptosis
rate were also determined in HepG2 and Caco-2 cells. Results: TiO2 NPs in a dose-
dependent manner increased cytotoxicity, oxidative stress, and apoptosis rate in
Caco-2 and HepG2 cells. The administration of TiO2 NPs significantly reduced
antioxidant enzyme activity and gene expressions (SOD, CAT, and GPx) as well as
glutathione (GSH) levels and total antioxidant capacity (TAC) in a dose-dependent
manner. TiO2 NPs also induced the apoptosis pathway and inflammatory pathway gene
expressions and caspase-3 activity in the intestine and liver. TUNEL assay was in
agreement with gene expressions. TiO2 NPs also led to morphological changes in the
liver and intestine. Conclusion: TiO2 NPs could have cytotoxic effects on the
intestine and liver structure and function by inducing oxidative stress,
inflammation, and apoptosis.
AN - WOS:000461298100001
AU - Abbasi-Oshaghi, E.
AU - Mirzaei, F.
AU - Pourjafar, M.
DO - 10.2147/IJN.S192382
PY - 2019
SN - 1178-2013
SP - 1919-1936
ST - NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine
and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro
study
T2 - INTERNATIONAL JOURNAL OF NANOMEDICINE
TI - NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine
and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro
study
VL - 14
ID - 6380
ER -
TY - JOUR
AB - The effects of ingestion of engineered nanoparticles (NPs), especially via
drinking water, are unknown. Using NPs spiked into synthetic water and cell culture
media, we investigated cell death, oxidative stress, and inflammatory effects of
silver (Ag), titanium dioxide (TiO2), and zinc oxide (ZnO) NPs on human intestinal
Caco-2 and SW480 cells. ZnO NPs were cytotoxic to both cell lines, while Ag and
TiO2 NPs were toxic only at 100 mg/L to Caco-2 and SW480, respectively. ZnO NPs led
to significant cell death in synthetic freshwaters with 1 % phosphate-buffered
saline in both cell lines, while Ag and TiO2 NPs in buffered water led to cell
death in SW480 cells. NP exposures did not yield significant increased reactive
oxygen species generation but all NP exposures led to increased IL-8 cytokine
generation in both cell lines. These results indicate cell stress and cell death
from NP exposures, with a varied response based on NP composition. © 2013 Springer
Science+Business Media Dordrecht.
AU - Abbott Chalew, T. E.
AU - Schwab, K. J.
DB - Scopus
DO - 10.1007/s10565-013-9241-6
IS - 2
KW - Nanoparticles
Silver
Titanium dioxide
Zinc oxide
Apoptosis
Caco-2 Cells
Cell Line, Tumor
Cell Proliferation
Cell Survival
Epithelial Cells
Humans
Inflammation
Interleukin-8
Intestinal Mucosa
Metal Nanoparticles
Oxidative Stress
Reactive Oxygen Species
Titanium
Zinc Oxide
interleukin 8
nanoparticle
reactive oxygen metabolite
silver nanoparticle
titanium dioxide nanoparticle
unclassified drug
zinc oxide nanoparticle
apoptosis
article
cell strain CACO 2
cell viability
concentration (parameters)
controlled study
culture medium
cytokine production
cytotoxicity
epithelium cell
human
human cell
in vitro study
oxidative stress
priority journal
M3 - Article
PY - 2013
SP - 101-116
ST - Toxicity of commercially available engineered nanoparticles to Caco-2 and
SW480 human intestinal epithelial cells
T2 - Cell Biology and Toxicology
TI - Toxicity of commercially available engineered nanoparticles to Caco-2 and
84878922261&doi=10.1007%2fs10565-013-9241-
6&partnerID=40&md5=3ab132f43743c853824b9513a46b7e70
VL - 29
ID - 5673
ER -
TY - JOUR
AB - The biosynthesis of metal nanoparticles using plant extracts is an eco-
friendly and inexpensive solution that has strong potential and applications in
science and industry. This study aims to synthesize Cu, Ag, and Au monometallic and
trimetallic nanoparticles (NPs) using the extracted polysaccharides (PS) of Vossia
cuspidata (Roxb.) Griff. leaves. Besides, the anti-cancer, anti-inflammatory, and
wound healing potentials of the synthesized NPs were tested. The synthesized NPs
were characterized using standard technological methods. We succeeded in green
synthesizing CuO, Ag, Au, monometallic, and CuO-Ag-Au trimetallic NPs. The
synthesized NPs had weak cytotoxicity at low concentrations (6.5 mu g/ml), but the
viability of cancer cells was reduced by increasing the concentration, suggesting
that the synthesized NPs have potent anti-cancer properties against the cells. The
synthesized NPs had 19.44-45.9 mu g/ml cytotoxic activity (IC50) against the MCF-7
cell line, 16.50-51.92 mu g/ml against A549, and 115.90-165.9 mu g/ml for normal
lung cells (WI-38). TMNPs were the most effective cytotoxic agents against all the
tested cell lines, followed by AuNPs on MCF-7 and CuONPs on A549. The cotton
fabric-treated TMNPs and CuONPs exhibited anti-inflammatory properties greater than
fabric-treated AgNPs and AuNPs and showed the highest odema inhibition (84.61% and
79.28%, respectively). In the wound healing assay, CuONPs and TMNPs caused the
highest percentages of inhibition (87.82% and 61.98%, respectively) for the wound
compared to AgNPs and AuNPs. TMNPs and CuONPs were more efficient in restoring the
tissue integrity of wounds than AgNPs and AuNPs. Accordingly, we recommend using
TMNPs and CuONPs in the wound healing dressings.
AN - WOS:000920974100001
AU - Abd El-Aziz, S. M.
AU - Farahat, E. A.
C6 - JAN 2023
DA - MAR
DO - 10.1007/s10904-023-02542-x
IS - 3
PY - 2023
SN - 1574-1443
1574-1451
SP - 853-865
ST - The Activity of Vossia cuspidata Polysaccharides-Derived Monometallic CuO,
Ag, Au, and Trimetallic CuO-Ag-Au Nanoparticles Against Cancer, Inflammation, and
Wound Healing
T2 - JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
TI - The Activity of Vossia cuspidata Polysaccharides-Derived Monometallic CuO,
Ag, Au, and Trimetallic CuO-Ag-Au Nanoparticles Against Cancer, Inflammation, and
Wound Healing
VL - 33
ID - 6300
ER -
TY - JOUR
AB - Oral candidiasis (OC) is a fungal infection caused by an opportunistic fungi
Candida albicans, which is found in the normal flora of healthy people. In this
study, we examined the anti-candidal effect of green synthesized silver
nanoparticles using leaf extract of Erodium glaucophyllum (EGAgNPs) against C.
albicans in vitro and in vivo. EG-AgNPs were synthesized for the first time using
E. glaucophyllum extract and characterized by imaging (transmission electron
microscopy (TEM), UV-VIS spectroscopy, zeta potential, X-ray diffraction (XRD),
Energy dispersive x-ray analysis (EDX), and Fourier transform infrared spectroscopy
(FTIR). A mouse model of OC was used for in vivo study. The agar well diffusion
method showed the anti-candidal activity of EG-AgNPs against C. albicans with MIC
50 µg/mL. EG-AgNPs inhibited the dimorphic transition of C. albicans and suppressed
the formation of biofilm by 56.36% and 52%, respectively. Additionally, EG-AgNPs
significantly inhibited the production of phospholipases and proteinases by 30% and
45%, respectively. EG-AgNPs cause cytoplasm disintegration and deterioration of
cell wall as imaged by SEM and TEM. Interestingly, EG-AgNPs did not display any
cytotoxicity on the human gingival fibroblast-1 HGF-1 cell line at MIC
concentrations. Topical treatment of the tongue of the OC mouse model with EG-AgNPs
showed significant reduction in candidal tissue invasion, less inflammatory
changes, and no tissue modification, in association with marked low scare and
hyphal counts as compared to control group. In conclusion, our data demonstrated
the potent inhibitory action of EG-AgNPs on the growth and morphogenesis of C.
albicans in vitro and in vivo. Thus, EG-AgNPs represent a novel plausible
therapeutic approach for treatment of OC. © 2022 by the authors. Licensee MDPI,
Basel, Switzerland.
AU - Abdallah, B. M.
AU - Ali, E. M.
C7 - 4221
DB - Scopus
DO - 10.3390/molecules27134221
IS - 13
KW - C. albicans
Erodium glaucophyllum
oral candidiasis
plant extract
silver nanoparticles
Animals
Anti-Bacterial Agents
Candida
Candida albicans
Candidiasis, Oral
Humans
Metal Nanoparticles
Mice
Plant Extracts
Silver
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
antiinfective agent
metal nanoparticle
silver
animal
chemistry
human
infrared spectroscopy
metabolism
mouse
thrush
X ray diffraction
M3 - Article
PY - 2022
ST - Therapeutic Effect of Green Synthesized Silver Nanoparticles Using Erodium
glaucophyllum Extract against Oral Candidiasis: In Vitro and In Vivo Study
T2 - Molecules
TI - Therapeutic Effect of Green Synthesized Silver Nanoparticles Using Erodium
glaucophyllum Extract against Oral Candidiasis: In Vitro and In Vivo Study
85133483409&doi=10.3390%2fmolecules27134221&partnerID=40&md5=874eff633a9fcaa7546195
cab6acf3cd
VL - 27
ID - 5078
ER -
TY - JOUR
AB - Trichophyton rubrum is the most common dermatophyte, and can cause cutaneous
infections in humans and animals (dermatophytosis). In this study, we investigated
the anti-dermatophytic potential of green synthesized silver nanoparticles using
Achillea santolina extract (AS-AgNPs) in an in vitro and in vivo rat model of
dermal T. rubrum dermatophytosis (TRD). The green synthesis of AS-AgNPs was
performed using A. santolina extract and characterized by UV-VIS spectroscopy, zeta
potential, imaging (transmission electron microscopy (TEM), X-ray diffraction
(XRD), Fourier transform infrared spectroscopy (FTIR) and Energy dispersive X-ray
analysis (EDX). The antifungal activity of AS-AgNPs was determined by the broth
microdilution method, conidial germination, and hyphal growth inhibition. TEM and
SEM were used to study the mode of the antifungal action of AS-AgNPs. AS-AgNPs
inhibited the growth of T. rubrum with an MIC of 128 μg/mL, and suppressed the
conidial germination and hyphal growth by 55.3% 84.6%, respectively. AS-AgNPs
caused modified mycelial structures, increased cell membrane permeability, and cell
wall damage. AS-AgNPs significantly increase the permeability of the fungal
membrane, as revealed by reducing ergosterol biosynthesis. An increase in the
intracellular ROS and the induction of apoptosis were also observed during AS-AgNP
treatment. In addition, AS-AgNPs reduced the cell wall integrity, as shown by the
reduction in the β-(1,3)-d-glucan synthase and chitin synthase activities. AS-AgNPs
showed very low toxicity on primary human dermal fibroblasts (HDF) at the MIC. The
topical treatment of the infected skin in the TRD rat model with AS-AgNPs showed a
significant reduction in the fugal burden after 7 days and a complete clearance of
fungal conidia, with a high recovery of epidermal and dermal structures after 14
days, compared to control rats. Interestingly, AS-AgNPs significantly attenuated
the infiltrated inflammatory cells, in association with reducing the tissue
proinflammatory cytokines including TNF-α, IL-1, IL-6, MOP and IL-17. In
conclusion, our data prove AS-AgNPs to be a novel green topical therapy for
dermatophytosis caused by T. rubrum. © 2023 by the authors.
AU - Rajendran, P.
AU - Ali, E. M.
C7 - 1536
DB - Scopus
DO - 10.3390/molecules28041536
IS - 4
KW - Achillea santolina
dermatophytosis
Trichophyton rubrum
Achillea
Animals
Antifungal Agents
Arthrodermataceae
Humans
Metal Nanoparticles
Plant Extracts
Rats
Silver
Tinea
X-Ray Diffraction
antifungal agent
metal nanoparticle
plant extract
silver
animal
chemistry
human
rat
tinea
X ray diffraction
M3 - Article
PY - 2023
ST - Potential Treatment of Dermatophyte Trichophyton rubrum in Rat Model Using
Topical Green Biosynthesized Silver Nanoparticles with Achillea santolina Extract
T2 - Molecules
TI - Potential Treatment of Dermatophyte Trichophyton rubrum in Rat Model Using
Topical Green Biosynthesized Silver Nanoparticles with Achillea santolina Extract
85149053889&doi=10.3390%2fmolecules28041536&partnerID=40&md5=fce7944232362f6ec3c929
1296bb19be
VL - 28
ID - 5000
ER -
TY - JOUR
AB - Background: Metals are trace elements, vital in some instances or toxic in
others. Due to this toxicity, they have been used since ancient time as
antimicrobials, and prescribed when plant-only remedies were not efficient enough.
These remedies could still contain secrets that may lead to the discovery of new
therapeutically interesting combinations. The objective of this study was to give a
proof of concept that such remedies combining metals and plants are worth studying
again. Methods: We exploited 4 medical formularies (aqrābādhīn), from three Arab
authors from the 9-12th century. We reproduced a remedy, and analyzed the role of
each ingredient. We further looked for the minimum inhibitory concentration against
three pathogenic bacteria, and we analyzed toxic and inflammatory effects of this
remedy on macrophages. Results: Even if plants were extensively used (almost 80 %
of all ingredients), more than 36 different minerals have been found in these 4
aqrābādhīn. When it came to remedies against infections that could be applied
externally, the use of metals grew to 70 %. We focused on a remedy, containing
mainly metals. We have been able to attribute a role for each ingredient, to show
that this skin remedy helped to combat the infection and to resorb the wound, and
to highlight the mastering of metal transformation by these physicians.
Conclusions: With a very simple recipe, mainly composed of metals, these past
physicians designed a complete and synergistic remedy to combat abscesses, while
restricting the toxic effect of metals to the site of infection. It is a first
example showing that different metal manufactures were evolved to improve their
therapeutic potentials. The knowledge acquired by these physician should deserve
more attention, and unexpected features, original organo-metallic compounds or
therapeutic synergy could still be found from such research. © 2022 Elsevier GmbH
AU - Abdallah, B.
AU - Seguin, C.
AU - Aubert, E.
AU - Ait BenHassou, H.
AU - Sbabou, L.
AU - Choulier, L.
AU - Vonthron, C.
AU - Schalk, I. J.
AU - Mislin, G. L. A.
AU - Fournel, S.
AU - Pitchon, V.
AU - Fechter, P.
C7 - 126926
DB - Scopus
DO - 10.1016/j.jtemb.2022.126926
KW - Antimicrobial
Inflammation
Metals
Past pharmacopeia
Wound healing
alkali
ammonia
antimony
antimony sulfide
borate sodium
cadmium
calamine
calcium oxide
calcium sulfate
copper
ferric oxide
gold
lead oxide
lead sulfide
mercury
mercury sulfide
potassium carbonate
potassium nitrate
pyrite
silver
sodium carbonate
sulfur
sulfuric acid
tobramycin
trace element
tumor necrosis factor
unclassified drug
antibacterial activity
antiinflammatory activity
Article
bacteriostatic activity
biotransformation
cell proliferation
cell viability
clinical feature
coughing
cytokine production
cytotoxicity
disk diffusion
drug formulation
Escherichia coli
human
macrophage
minimum inhibitory concentration
nonhuman
proof of concept
Pseudomonas aeruginosa
pustule
pyrolysis
respiratory tract infection
skin abscess
skin fistula
skin infection
skin ulcer
Staphylococcus aureus
thorax pain
tuberculous lymphadenitis
urinary tract infection
zone of inhibition
M3 - Article
PY - 2022
ST - Past mastering of metal transformation enabled physicians to increase their
therapeutic potential
T2 - Journal of Trace Elements in Medicine and Biology
TI - Past mastering of metal transformation enabled physicians to increase their
therapeutic potential
85122648783&doi=10.1016%2fj.jtemb.2022.126926&partnerID=40&md5=885afe25544bd30c42b9
b20c2827ffce
VL - 71
ID - 5102
ER -
TY - JOUR
AB - The unlimited use of nanoparticles (NPs) results in toxic impacts on
different tissues. The current study aimed to compare the adverse effects of AgNPs
and TiO2NPs on the parotid gland of adult male albino rats as regards the
histopathological, immunohistochemical, and biochemical changes, exploring the
possible underlying mechanisms and the degree of improvement after cessation of
administration. Fifty-four adult male albino rats were divided into control group
(I), AgNPs-injected group (II), and TiO2NPs-injected group (III). We measured the
levels of tumor necrosis factor-alpha (TNF-α) and interleukin (IL-6) in the serum,
and levels of MDA and GSH in parotid tissue homogenate. Quantitative real-time
polymerase-chain reaction (qRT-PCR) was used to measure the expression levels of
peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1-α),
nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), mouse double minute 2
(MDM2), Caspase-3 Col1a1, and Occludin. Parotid tissue sections were examined by
light microscope (Hematoxylin & Eosin and Mallory trichrome stains), electron
microscope, and immunohistochemical examination of CD68 and anti-caspase-3
antibodies. Both NPs severely affected the acinar cells and damaged the tight
junction between them by enhancing expression of the inflammatory cytokines,
inducing oxidative stress, and disturbing the expression levels of the studied
genes. They also stimulated fibrosis, acinar cell apoptosis, and inflammatory cells
infiltration in parotid tissue. TiO2NPs effects were less severe than AgNPs.
Cessation of exposure to both NPs, ameliorated the biochemical and structural
findings with more improvement in TiO2NPs withdrawal. In conclusion: AgNPs and
TiO2NPs adversely affected the parotid gland, but TiO2NPs were less toxic than
AgNPs. © 2023 Taylor & Francis Group, LLC.
AU - Abdel Aal, S. M.
AU - Mohammed, M. Z.
AU - Samy, W.
AU - Abdelaal, G. M. M.
AU - Deraz, R. H.
DB - Scopus
DO - 10.1080/01913123.2023.2205924
IS - 4
KW - Biochemical, rats
histological
parotid gland
Silver nanoparticles (AgNPs)
Titanium dioxide nanoparticles (TiO2NPs)
Animals
Male
Metal Nanoparticles
Mice
Nanoparticles
Parotid Gland
Silver
Titanium
metal nanoparticle
nanoparticle
silver
titanium
titanium dioxide
animal
male
mouse
M3 - Article
PY - 2023
SP - 339-363
ST - Histological and biochemical evaluation of the effects of silver
nanoparticles (AgNps) versus titanium dioxide nanoparticles (TiO2NPs) on rat
parotid gland
T2 - Ultrastructural Pathology
TI - Histological and biochemical evaluation of the effects of silver
nanoparticles (AgNps) versus titanium dioxide nanoparticles (TiO2NPs) on rat
parotid gland
85158878833&doi=10.1080%2f01913123.2023.2205924&partnerID=40&md5=b48071534db69b4891
3bbc17726d6ee5
VL - 47
ID - 5034
ER -
TY - JOUR
AB - Background The green synthesis of metal and metal oxide nanoparticles (NPs),
notably from plants, has attracted increasing attention in recent years. Although
the increased popularity use of Cymbopogon citratus as a therapeutic substance, to
date, there has not been any research on the chemistry of C. citratus aqueous leaf
extract (ALE) or synthesis of ZnO NPs utilizing an extract from it. The
ecologically safe ALE of C. citratus was employed in this study as a bio-reducing
and capping agent to synthesize ZnO NPs. Results The novelty of the current study
is the investigation of the antioxidant, anti-inflammatory, anti-microbial, and
cytotoxic potencies of biosynthesized ZnO NPs utilizing C. citratus ALE. Zinc
acetate dihydrate was used as the precursor and the leaf extract serves as the
reducing agent. ZnO NPs from ALE of C. citratus were characterized by the spherical
in form by using high-resolution transmission electron microscopy (HR-TEM) and the
Scherrer formula was used to calculate the size of the crystalline structure. The
presence of numerous functional groups in both the ALE and the NPs is confirmed by
FTIR analysis. The highest absorption peak is observed at 370 nm. The stability and
particle size of the biosynthesized ZnO NPs are demonstrated by dynamic light
scattering (DLS) analysis. The biosynthesized ZnO NPs exhibited excellent
antioxidant activity with an IC50 value of 45.67 +/- 0.1 mu g/mL and exerted
interesting anti-inflammatory activity (98.1% +/- 0.04) when compared to the
standard indomethacin (92.1% +/- 0.07) at 1 mg/mL. They also showed anti-microbial
activity for both bacterial and fungal which growth rates for both significantly
decreased with the increase in ZnO NPs concentration compared to the control. The
anticancer activity of biosynthesized ZnO NPs and C. citratus ALE was in vitro
tested against seven human cancer cell lines (HCCL) (i.e. H1299, MDA-MB-468, HNO97,
HEK, HCT116, HuH7, and HEPG2) compared to normal cells (HSF) using the
sulforhodamine-B (SRB) assay. More interestingly, the biosynthesized ZnO NPs
displayed remarkable selective cytotoxicity against all tested cancer cell lines
without any effect on normal cells. In contrast, the cancer cell lines were not
affected by the ALE of C. citratus at any concentrations tested. Conclusions All
the findings confirm that the ZnO NPs biosynthesized in the current work are
promising candidates for a variety of biological activities, and as a result, they
can be helpful to the medical sector, environmental and agricultural applications.
AN - WOS:001033810700001
AU - Abdelbaky, A. S.
AU - Mohamed, Amha
AU - Sharaky, M.
AU - Mohamed, N. A.
AU - Diab, Y. M.
C7 - 63
DA - JUL 20
DO - 10.1186/s40538-023-00432-5
IS - 1
PY - 2023
SN - 2196-5641
ST - Green approach for the synthesis of ZnO nanoparticles using Cymbopogon
citratus aqueous leaf extract: characterization and evaluation of their biological
activities
T2 - CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
TI - Green approach for the synthesis of ZnO nanoparticles using Cymbopogon
citratus aqueous leaf extract: characterization and evaluation of their biological
activities
VL - 10
ID - 6275
ER -
TY - JOUR
AB - Tumor necrosis factor (TNF-α) and inflammatory cytokine (IL-6) play a vital
role in various cellular incidents such as the proliferation and death of cells
during carcinogenesis. Hence, regulation of these biomarkers could be a promising
tool for controlling tumor progression using nanoformulations. Silver
nanoparticles-poly (vinyl pyrrolidone) (AgNPs-PVP) were prepared using the
reduction of silver nitrate and stabilized with PVP. They are characterized through
yield percentage, UV-VIS, FT-IR, size, charge, and morphology. The obtained AgNPs
were tested for anticancer activity against prostate cancer (PC 3) and human skin
fibroblast (HFS) cell lines. Moreover, biomarker-based confirmations like TNF-α and
IL-6 were estimated. The synthesized AgNPs-PVP were stable, spherical in shape,
with particle sizes of 122.33 ± 17.61 nm, a polydispersity index of 0.49 ± 0.07,
and a negative surface charge of -19.23 ± 0.61 mV. In vitro cytotoxicity testing
showed the AgNPs-PVP exhibited antiproliferation properties in PC3 in a dose-
dependent manner. In addition, when compared to control cells, AgNPs-PVP has lower
TNF-α with a significant value (∗p<0.05); the value reached 16.84 ± 0.71 pg/ml
versus 20.81 ± 0.44 pg/ml, respectively. In addition, HSF cells showed a high level
of reduction (∗∗∗p<0.001) in IL-6 production. This study suggested that AgNPs-PVP
could be a possible therapeutic agent for human prostate cancer and anti-IL-6 in
cancerous and noncancerous cells. Further studies will be performed to investigate
the effect of AgNPs-PVP in different types of cancer. © 2022 Ahmed A. H. Abdellatif
et al.
AU - Abdellatif, A. A. H.
AU - Abdelfattah, A.
AU - Bouazzaoui, A.
AU - Osman, S. K.
AU - Al-Moraya, I. S.
AU - Showail, A. M. S.
AU - Alsharidah, M.
AU - Aboelela, A.
AU - Al Rugaie, O.
AU - Faris, T. M.
AU - Tawfeek, H. M.
C7 - 6181448
DB - Scopus
DO - 10.1155/2022/6181448
M3 - Article
PY - 2022
ST - Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential
Anticancer Activity towards Prostate Cancer
T2 - Bioinorganic Chemistry and Applications
TI - Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential
Anticancer Activity towards Prostate Cancer
85139723470&doi=10.1155%2f2022%2f6181448&partnerID=40&md5=0205460ea2e670b30a4cedf2f
93a6b6a
VL - 2022
ID - 5068
ER -
TY - JOUR
AB - OBJECTIVE: Silver nanoparticles (AgNPs) are known to exhibit anti-
inflammatory and anticancer activities. They have been reported to reduce the
levels of tumor necrosis factor (TNF) - alpha proinflammatory cytokine involved in
cell proliferation, differentiation, and apoptosis - in cell lines. As patients
with breast cancer have been reported to have higher serum TNF levels, we aimed at
developing a novel treatment for breast cancer by evaluating the effect of
Trigonella foenum-graecum extract (TFG)-reduced AgNPs on the MCF-7 cell line, which
serves as a model of human breast cancer. MATERIALS AND METHODS: TFG-capped AgNPs
were synthesized using a green reduction method, in which TFG reduced silver
nitrate to generate AgNPs-TFG. The particle size, surface charge, ultraviolet (UV)-
visible (VIS) spectra, surface morphology, % yield, and in vitro Ag+ release of the
formulated AgNPs-TFG were evaluated. Additionally, the prepared NPs were examined
for cytotoxicity using real-time polymerase chain reaction (real-time PCR), 3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and enzyme-
linked immunosorbent assay (ELISA). RESULTS: The prepared AgNPs-TFG were uniform,
small, discrete, and non-aggregated with a particle size of 22.5 +/- 0.75 nm and
zeta-potential of -47.45 +/- 0.666 mV. The yield of AgNPs-TFG was 224.545 +/- 3.9
mu M. Furthermore, the AgNP-TFG thin film exhibited a prolonged release of Ag+ in
phosphate buffer for up to 11 h. AgNPs-TFG suppressed TNF-alpha expression at mRNA
and protein levels in MCF-7 cells. Additionally, the formulated AgNPs-TFG did not
exhibit any toxicity toward MCF-7 cells. CONCLUSIONS: This study showed that Ag-NP-
TFG could effectively inhibit TNF-alpha. These results provide significant insights
for developing new therapeutic strategies for cancer and other inflammatory
illnesses.
AN - WOS:000864081200029
AU - Osman, S. K.
AU - Alsharidah, M.
AU - Al Rugaie, O.
AU - Faris, T. M.
AU - Alqasoumi, A.
AU - Mousa, A. M.
AU - Bouazzaoui, A.
DO - 10.26355/eurrev_202208_29424
IS - 15
PY - 2022
SN - 1128-3602
SP - 5529-5539
ST - Green synthesis of silver nanoparticles reduced with Trigonella foenum-
graecum and their effect on tumor necrosis factor-alpha in MCF7 cells
T2 - EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
TI - Green synthesis of silver nanoparticles reduced with Trigonella foenum-
graecum and their effect on tumor necrosis factor-alpha in MCF7 cells
VL - 26
ID - 5920
ER -
TY - JOUR
AB - OBJECTIVE: Silver nanoparticles (AgNPs) are known to exhibit anti-
inflammatory and anticancer activities. They have been reported to reduce the
levels of tumor necrosis factor (TNF) – a proinflammatory cytokine involved in cell
proliferation, differentiation, and apoptosis – in cell lines. As patients with
breast cancer have been reported to have higher serum TNF levels, we aimed at
developing a novel treatment for breast cancer by evaluating the effect of
Trigonella foenum-graecum extract (TFG)-reduced AgNPs on the MCF-7 cell line, which
serves as a model of human breast cancer. MATERIALS AND METHODS: TFG-capped AgNPs
were synthesized using a green reduction method, in which TFG reduced silver
nitrate to generate AgNPs-TFG. The particle size, surface charge, ultraviolet (UV)-
visible (VIS) spectra, surface morphology, % yield, and in vitro Ag+ release of the
formulated AgNPs-TFG were evaluated. Additionally, the prepared NPs were examined
for cytotoxicity using real-time polymerase chain reaction (real-time PCR), 3-(4,5-
dimethylth-iazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and enzyme-
linked immunosorbent assay (ELISA). RESULTS: The prepared AgNPs-TFG were uniform,
small, discrete, and non-aggregated with a particle size of 22.5±0.75 nm and ζ-
potential of -47.45±0.666 mV. The yield of AgNPs-TFG was 224.545±3.9 µM.
Furthermore, the AgNP-TFG thin film exhibited a prolonged release of Ag+ in
phosphate buffer for up to 11 h. AgNPs-TFG suppressed TNF-α expression at mRNA and
protein levels in MCF-7 cells. Additionally, the formulated AgNPs-TFG did not
exhibit any toxicity toward MCF-7 cells. CONCLUSIONS: This study showed that AgNP-
TFG could effectively inhibit TNF-α. These results provide significant insights for
developing new therapeutic strategies for cancer and other inflammatory illnesses.
© 2022 Verduci Editore s.r.l. All rights reserved.
AU - Osman, S. K.
AU - Alsharidah, M.
AU - Rugaie, O. A. L.
AU - Faris, T. M.
AU - Alqasoumi, A.
AU - Mousa, A. M.
AU - Bouazzaoui, A.
DB - Scopus
DO - 10.26355/eurrev_202208_29424
IS - 15
KW - MCF-7 cells
Silver nanoparticles
Trigonella foenum-graecum
Tumor necrosis factor-α
Breast Neoplasms
Female
Humans
MCF-7 Cells
Metal Nanoparticles
Plant Extracts
Silver
Trigonella
Tumor Necrosis Factor-alpha
fenugreek extract
silver nanoparticle
metal nanoparticle
plant extract
silver
antineoplastic activity
apoptosis
aqueous solution
Article
breast cancer
cell differentiation
cell proliferation
controlled study
cytokine release
cytotoxicity
enzyme linked immunosorbent assay
fenugreek
green chemistry
human
human cell
in vitro study
MCF-7 cell line
morphology
mRNA expression level
MTT assay
particle size
protein expression level
real time polymerase chain reaction
reduction (chemistry)
surface charge
synthesis
ultraviolet visible spectroscopy
breast tumor
female
pathology
M3 - Article
PY - 2022
SP - 5529-5539
ST - Green synthesis of silver nanoparticles reduced with Trigonella foenum-
graecum and their effect on tumor necrosis factor-α in MCF7 cells
T2 - European Review for Medical and Pharmacological Sciences
TI - Green synthesis of silver nanoparticles reduced with Trigonella foenum-
graecum and their effect on tumor necrosis factor-α in MCF7 cells
85135763768&doi=10.26355%2feurrev_202208_29424&partnerID=40&md5=9f09de6bf82ad990b85
53f39a93303c3
VL - 26
ID - 5147
ER -
TY - JOUR
AB - Background: The nuclear factor kappa-B (NF-κB) is a major transcription
factor respon-sible for the production of numerous inflammatory mediators,
including the tumor necrosis factor (TNFα), which has a lethal association with
cancer’s onset. The silver nanoparticles (AgNPs) are widely used in cancer
treatment and several other biomedical applications. Objective: The study aimed to
determine the effects of silver citrate nanoparticles (AgNPs-CIT) on NF-κB
activation together with TNFα mRNA/protein expressions in the phorbol myristate
acetate (PMA)-stimulated MCF-7 human breast cancer cell-lines. Methods: The AgNPs-
CIT were synthesized by the reduction method, and the prepared AgNPs-CIT were
characterized for their shape, absorption in UV-VIS electromagnetic radiations,
size distribution, ζ-potential, and antioxidant activity. The MCF-7 cell-lines were
pretreated with AgNPs-CIT and stimulated with PMA. The TNFα mRNA expressions were
determined by real-time PCR, whereas the protein production was determined by the
ELISA. The NF-κB activity was distinctly observed by highly-specific DNA-based
ELISA, and by NF-κB-specific inhibitor, Bay 11–7082. Results: The prepared AgNPs-
CIT were spherical and have an absorption wavelength range of 381–452 nm wherein
the particles size ranged between 19.2±0.1 to 220.77±0.12 nm with the charge range
−9.99±0.8 to −34.63±0.1 mV. The prepared AgNPs-CIT showed comparative antioxidant
activity at >40% inhibitions level of the DPPH radicals. The AgNPs-CIT were found
to be non-toxic to MCF-7 cell-lines and inhibited PMA-induced activation of the NF-
κBp65, and also the mRNA/protein expression of TNFα. Conclusion: This is the first
report that showed AgNPs-CIT inhibited TNFα expression via deactivation of the NF-
κB signaling event in stimulated breast cancer cells. The results have important
implications for the development of novel therapeutic strategies for the
prevention/ treatment of cancers and/or inflammatory disorders. © 2020 Abdellatif
et al.
AU - Rasheed, Z.
AU - Alhowail, A. H.
AU - Alqasoumi, A.
AU - Alsharidah, M.
AU - Khan, R. A.
AU - Aljohani, A. S. M.
AU - Aldubayan, M. A.
AU - Faisal, W.
DB - Scopus
DO - 10.2147/IJN.S274098
KW - Cancer
Inflammatory cytokines
MCF-7 cell lines
NF-κB
Phorbol myristate acetate
PMA
Silver citrate nanoparticles
TNFα
Citric Acid
Free Radical Scavengers
Gene Expression Regulation, Neoplastic
Humans
MCF-7 Cells
Metal Nanoparticles
NF-kappa B
Particle Size
Silver
Spectrophotometry, Ultraviolet
Static Electricity
Tetradecanoylphorbol Acetate
1,1 diphenyl 2 picrylhydrazyl
3 (4 methylphenylsulfonyl) 2 propenenitrile
citrate trisodium
immunoglobulin enhancer binding protein
messenger RNA
phorbol 13 acetate 12 myristate
silver citrate nanoparticle
silver nanoparticle
transcription factor RelA
unclassified drug
citric acid
metal nanoparticle
scavenger
silver
absorption
antioxidant activity
Article
cell stimulation
controlled study
drug cytotoxicity
electron microscopy
Fourier transform infrared spectroscopy
gene expression
human
human cell
MCF-7 cell line
particle size
photon correlation spectroscopy
physical parameters
protein expression
protein function
protein synthesis
signal transduction
surface property
synthesis
tumor necrosis factor alpha gene
zeta potential
chemistry
drug effect
gene expression regulation
genetics
metabolism
static electricity
ultraviolet spectrophotometry
M3 - Article
PY - 2020
SP - 8479-8493
ST - Silver citrate nanoparticles inhibit pma-induced tnfα expression via
deactivation of nf-κb activity in human cancer cell-lines, mcf-7
T2 - International Journal of Nanomedicine
TI - Silver citrate nanoparticles inhibit pma-induced tnfα expression via
deactivation of nf-κb activity in human cancer cell-lines, mcf-7
85094667691&doi=10.2147%2fIJN.S274098&partnerID=40&md5=683a14545e22fb97b0fcda6fb4c0
7429
VL - 15
ID - 5330
ER -
TY - JOUR
AB - Exposure to the deleterious effects of silver nanoparticles (AgNPs) is
inevitable due to their wide use in medicine and daily life. The current study
aimed to delineate the histomorphological changes and the molecular mechanisms
underlying the ameliorative effect of Resveratrol (RSV) on rats' livers exposed to
AgNPs. Fifty healthy adult male Wistar albino rats were divided into four groups:
control, AgNPs-exposed, RSV-treated after AgNPs exposure, and recovery groups.
Liver sections were examined by light and electron microscopes, and
immunohistochemistry was performed for detection of activated caspase3 and TNF
alpha. Serum ALT and AST, plasma levels of TNF alpha, IL-6, GSH and SOD were
measured. mRNA expression of SIRT1, ADORA3, PAI, CDK1, Nrf2 and NF kappa B genes in
liver tissue homogenate was performed using qRT-PCR. AgNPs-exposure for 28 days
caused marked liver tissue damage with trapping in hepatocytes and Kupffer cells,
while RSV treatment ameliorated liver ultrastructure and function. Our results
clarified the molecular basis of RSV ameliorative effect on liver tissue by
significant upregulation of SIRT1-NrF2 signaling pathway with increased levels of
the antioxidant GSH and SOD, which represent the antioxidant effect of RSV.
Significant upregulation of the protective ADORA3 with downregulation of the
proinflammatory PAI-1 and NF kappa B mRNA expression levels besides decreased
plasma levels of TNF alpha, IL-6 and decreased immunoexpression of TNF alpha in
liver tissue, represent the anti-inflammatory effect of RSV. In addition, decreased
immunoexpression of caspase3 and downregulation of CDK1 expression, represent its
antiapoptotic effect. In conclusion: RSV ameliorates AgNPs-induced liver damage by
antioxidant, anti-inflammatory and antiapoptotic effects.
AN - WOS:000787719100001
AU - Mahmoud, A. A.
AU - Samy, W.
AU - Saleh, E. Z. H.
C6 - APR 2022
DA - MAY 4
DO - 10.1080/01913123.2022.2067929
IS - 3
PY - 2022
SN - 0191-3123
1521-0758
SP - 268-284
ST - Histomorphological changes and molecular mechanisms underlying the
ameliorative effect of resveratrol on the liver of silver nanoparticles-exposed
rats
TI - Histomorphological changes and molecular mechanisms underlying the
ameliorative effect of resveratrol on the liver of silver nanoparticles-exposed
rats
VL - 46
ID - 5962
ER -
TY - JOUR
AB - Purpose: Nowadays, there is a dramatic increase in the interest of potential
impact of consumer-relevant engineered nanoparticles on pregnancy. Materials and
methods: This study investigated the possible protective effect of montelukast in
neonatal organ toxicity induced by maternal exposure to silver nanoparticles
(AgNPs) in rats. Results: It was noticed that montelukast reduced serum urea,
creatinine, renal caspase-3 immunoreactivity and IL-1β and increased total
antioxidant capacity, as compared to AgNPs. In kidney and bone tissue, montelukast
reduced oxidative stress parameters and TNF-α level that was increased with AgNPs.
Surprisingly, montelukast administration increased epidermal growth factor (EGF) in
bone and reduced it in kidney. Furthermore, as compared to AgNPs, montelukast
improved histopathological picture of kidney and bone. Conclusions: In conclusion,
montelukast antagonized the biochemical and histopathological changes occurred in
kidneys and bones of rat offspring by maternal exposure to AgNPs, mostly by anti-
oxidant, anti-apoptotic and anti-inflammatory actions with a possible role for EGF.
© 2021 Informa UK Limited, trading as Taylor & Francis Group.
AU - Abdelzaher, W. Y.
AU - Rofaeil, R. R.
AU - Abdel-Hafez, S. M. N.
AU - Atta, M.
AU - Bahaa El-deen, M. A.
AU - Ali, D. M.
DB - Scopus
DO - 10.1080/08923973.2021.1878213
IS - 2
KW - Epidermal growth factor
montelukast
multi-organ toxicity
rat offspring
Acetates
Animals
Calcification, Physiologic
Cyclopropanes
Epidermal Growth Factor
Female
Kidney
Leukotriene Antagonists
Male
Metal Nanoparticles
Oxidative Stress
Pregnancy
Prenatal Exposure Delayed Effects
Quinolines
Rats
Rats, Sprague-Dawley
Receptors, Leukotriene
Silver
Sulfides
caspase 3
creatinine
epidermal growth factor
interleukin 1beta
malonaldehyde
silver nanoparticle
acetic acid
cyclopropane derivative
leukotriene receptor
leukotriene receptor blocking agent
metal nanoparticle
quinoline derivative
silver
sulfide
animal experiment
animal model
animal tissue
Article
blood sampling
bone tissue
cell proliferation
chow diet
colorimetry
comparative study
controlled study
decapitation
female
histopathology
immunohistochemistry
immunoreactivity
kidney cortex tissue
kidney proximal tubule
kidney tissue
maternal exposure
morphometry
newborn
nonhuman
oxidative stress
particle size
pregnancy
priority journal
rat
tissue homogenate
animal
bone mineralization
drug effect
drug therapy
kidney
male
metabolism
pathology
physiology
prenatal exposure
Sprague Dawley rat
M3 - Article
PY - 2021
SP - 183-191
ST - Ameliorating effect of leukotriene receptor antagonist in multi-organ
toxicity induced in rat offspring, a possible role for epidermal growth factor
T2 - Immunopharmacology and Immunotoxicology
TI - Ameliorating effect of leukotriene receptor antagonist in multi-organ
85100045035&doi=10.1080%2f08923973.2021.1878213&partnerID=40&md5=2f63b851732b83dea6
399d84462ae567
VL - 43
ID - 5289
ER -
TY - JOUR
AB - Purpose: Nowadays, there is a dramatic increase in the interest of potential
impact of consumer-relevant engineered nanoparticles on pregnancy. Materials and
methods: This study investigated the possible protective effect of montelukast in
neonatal organ toxicity induced by maternal exposure to silver nanoparticles
(AgNPs) in rats. Results: It was noticed that montelukast reduced serum urea,
creatinine, renal caspase-3 immunoreactivity and IL-1 beta and increased total
antioxidant capacity, as compared to AgNPs. In kidney and bone tissue, montelukast
reduced oxidative stress parameters and TNF-alpha level that was increased with
AgNPs. Surprisingly, montelukast administration increased epidermal growth factor
(EGF) in bone and reduced it in kidney. Furthermore, as compared to AgNPs,
montelukast improved histopathological picture of kidney and bone. Conclusions: In
conclusion, montelukast antagonized the biochemical and histopathological changes
occurred in kidneys and bones of rat offspring by maternal exposure to AgNPs,
mostly by anti-oxidant, anti-apoptotic and anti-inflammatory actions with a
possible role for EGF.
AN - WOS:000612451800001
AU - Abdelzaher, W. Y.
AU - Rofaeil, R. R.
AU - Abdel-Hafez, S. M. N.
AU - Atta, M.
AU - El-deen, M. A. B.
AU - Ali, D. M.
C6 - JAN 2021
DA - MAR 4
DO - 10.1080/08923973.2021.1878213
IS - 2
PY - 2021
SN - 0892-3973
1532-2513
SP - 183-191
ST - Ameliorating effect of leukotriene receptor antagonist in multi-organ
T2 - IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
TI - Ameliorating effect of leukotriene receptor antagonist in multi-organ
VL - 43
ID - 6096
ER -
TY - JOUR
AB - In the present study, fish were exposed to sub‐lethal doses of CuONPs (68.92
± 3.49 nm) (10 mg/L, 20 mg/L, and 50 mg/L) for a long exposure period (25 days).
Compared to the control group (0.0 mg/L CuONPs), a significant dose‐dependent
elevation in blood urea and creatinine val-ues, serum alanine transaminase,
aspartate transaminase, and alkaline phosphatase enzyme activities were evident in
CuONPs‐exposed groups (p < 0.05). Fish exposure to 50 mg/L CuONPs significantly
upregulated the transcription of pro‐inflammatory cytokines (tumor necrosis factor‐
alpha, interleukin‐1beta, interleukin 12, and interleukin 8), heat shock protein
70, apoptosis‐related gene (caspase 3), and oxidative stress‐related (superoxide
dismutase, catalase, and glutathione peroxi-dase) genes in liver and gills of the
exposed fish in comparison with those in the control group (p < 0.05). Moreover,
varying histopathological injuries were noticed in the hepatopancreatic tissues,
posterior kidneys, and gills of fish groups correlated to the tested exposure dose
of CuONPs. In summary, our results provide new insights and helpful information for
better understanding the mechanisms of CuONPs toxicity in Nile tilapia at
hematological, molecular levels, and tissue levels. © 2021 by the authors. Licensee
MDPI, Basel, Switzerland.
AU - Abdel‐latif, H. M. R.
AU - Dawood, M. A. O.
AU - Mahmoud, S. F.
AU - Shukry, M.
AU - Noreldin, A. E.
AU - Ghetas, H. A.
AU - Khallaf, M. A.
C7 - 652
DB - Scopus
DO - 10.3390/ani11030652
IS - 3
KW - CuONPs
Gene transcription
Histopathology
Nile tilapia
Toxicity
alanine aminotransferase
alkaline phosphatase
aspartate aminotransferase
caspase
caspase 3
catalase
copper nanoparticle
copper oxide nanoparticle
creatinine
cytokine
glutathione
glutathione peroxidase
glutathione reductase
glutathione transferase
heat shock protein 70
interleukin 12
interleukin 1beta
interleukin 8
nanoparticle
silver nanoparticle
superoxide dismutase
alanine aminotransferase blood level
animal experiment
animal model
animal tissue
Article
cancer mortality
catfish
Clarias gariepinus
controlled study
DNA fragmentation
enzyme activity
epithelium hyperplasia
fish
gene expression
histopathology
injury
kidney
LC50
liver toxicity
myocarditis
necrosis
nonhuman
Oreochromis niloticus
oxidative stress
scanning electron microscopy
sublethal dose
tissue level
transcription initiation
transmission electron microscopy
urea blood level
zebra fish
zeta potential
M3 - Article
PY - 2021
SP - 1-21
ST - Copper oxide nanoparticles alter serum biochemical indices, induce
histopathological alterations, and modulate transcription of cytokines, hsp70, and
oxidative stress genes in oreochromis niloticus
T2 - Animals
TI - Copper oxide nanoparticles alter serum biochemical indices, induce
histopathological alterations, and modulate transcription of cytokines, hsp70, and
oxidative stress genes in oreochromis niloticus
85101717517&doi=10.3390%2fani11030652&partnerID=40&md5=0c6bde7a528d21c8899e073804ee
e875
VL - 11
ID - 5227
ER -
TY - JOUR
AB - Aim of investigation. To study of possibilities of endoscopic treatment of
vesicourethral reflux in children. Material & methods. During the period from 2002
until 2005 the endoscopic correction of vesicourethral reflux (VUR) have been
performed in 36 children at the age between 1.7-15 y.o. suffering from first,
second and third degrees of VUR (9 children had bilateral VUR). The statistics of
the urethral refluxes was as follows: first degree VUR - 11,1% (five ureters);
seond degree - 62,2% (28 ureters); third degree - 26,7% (12 ureters). In the
capacity of the implantant was used polyacrilamidal biopolymeral highly viscous gel
containing silver ions - "Argiform" (production of "Bioform", Russia). The gel
possesses constant action, does not dissolve, is not rejected by tissues, and does
not tend to migrate in the tissues. The implantation of the gel was being done
under the general anesthesia. The duration of unilateral endoscopic correction did
not exceed 15-20 minutes, and of bilateral correction - 30-40 minutes. The patients
stayed in hospital only one day. In our further work the implantation was being
done without hospitalization. Results. The results were followed up right after the
implantation. The remote results were followed up in 34 patients (94,4%): they were
examined after a long period - more than two years. In 95,3% of cases was achieved
total liquidation of reflux. In two ureters the severity of reflux was diminished
from third to first degree. There were no complications after the implantation of
gel, not a single case of obstructive post-implantation syndrome or aggravation of
inflammatory process in the urinary tract. The obtained data allow to conclude that
the endoscopic correction of VUR in children by means of injected implantation of
biopolymeral gel "Argiform" is noninvasive, takes little time, does not demand long
lasting hospitalization, is highly efficacious, and economically profitable.
AU - Abdullaev, K. I.
AU - Akbarov, T. R.
DB - Scopus
IS - 1
KW - Endoscopic correction
Vesicourethral reflux
polyacrylamide gel
silver
adolescent
article
biocompatibility
child
clinical article
cost effectiveness analysis
disease exacerbation
disease severity
endoscopic surgery
follow up
general anesthesia
hospitalization
human
non invasive measurement
operation duration
treatment outcome
ureter obstruction
urinary tract inflammation
vesicoureteral reflux
M3 - Article
PY - 2005
SP - 110-114
ST - Endoscopic treatment of vesicourethral reflux in children
T2 - Azerbaijan Medical Journal
TI - Endoscopic treatment of vesicourethral reflux in children
18744386716&partnerID=40&md5=4db6f2a9419ca52df2d77a8472542b7a
ID - 5778
ER -
TY - GEN
AB - O osso homógeno fresco congelado tem sido utilizado no intuito de substituir
o osso autógeno. No entanto as suas propriedades de osteoindução e até mesmo
osteocondução não estão bem conceituadas na literatura científica. Esse trabalho
teve por objetivo avaliar amostras de tecido ósseo homógeno enxertados em humanos
através das análises qualitativas histológica e imunoistoquímica. Para tal, dez
pacientes previamente selecionados foram submetidos à cirurgia de reconstrução de
defeitos ósseos em espessura, usando enxerto ósseo homógeno em bloco fresco
congelado estabilizado e fixado por meio de parafusos bicorticais. Após período de
integração de seia meses foi realizado procedimento de reabertura para instalação
dos implantes osseointegráveis. Neste momento cirúrgico, amostras do enxerto ósseo
foram removidas por meio de broca trefina. As amostras foram fixadas em formol 10%,
levadas ao processamento para corte descalcificado em parafina e coradas por
hematoxilina e eosina. Realizou-se processamento imunoistoquímico para a expressão
da enzima Caspase 3. As lâminas foram levadas à microscopia óptica para realização
das análises histológica e imunoistoquímica qualitativas. Os resultados mostraram
tecido ósseo não vital, com poucas áreas de deposição de osso neoformado sobre a
matriz amorfa, presença de infiltrado inflamatório com áreas de osteomielite e
imunomarcação expressiva da enzima Caspase 3. Diante da metodologia empregada e dos
resultados obtidos concluiu-se que o enxerto homógeno apresentou somente a
propriedade biológica de osteocondução durante a fase de incorporação de seis meses
Allogeneic bone has been used fresh frozen in order to replace bone autograft.
However, the properties of osteoinduction and osteoconduction are not even well-
regarded in the scientific literature. This work aimed to evaluate samples of
homogenous bone grafts in humans by qualitative histological analysis and
immunohistochemistry. For this ten pre-selected patients underwent surgical
reconstruction of bone defects in thick, homogenous bone graft using fresh frozen
block stabilized and fixed by bicortical screws. After integration period of six
months reopening procedure was performed for installation of osseointegrated
implants. At this surgical time bone graft samples were removed by means of drill
trephine. The samples were fixed in formalin 10%, taken to court for processing
decalcified paraffin, and stained with hematoxylin and eosin. Immunohistochemistry
was performed processing for the expression of the enzyme Caspase 3. The slides
were brought to light microscopy to carry out the qualitative histology and
immunohistochemistry. The results showed non-vital bone tissue, with few areas of
deposition of new bone formation on the amorphous matrix, presence of chronic
inflammatory infiltrate with areas of osteomyelitis, and immunostaining expressive
of the enzyme Caspase 3. Given the methods employed and the results obtained it was
concluded that the graft showed only a homogenous biological property of
osteoconduction, during the incorporation of six months
AU - Abla, Marcelo Sabbag
C1 - 20140326
C8 - lil-711313
DA - 2012/00
DB - LILACS
DP - http://bvsalud.org/
KW - Alogenic bone
Biocompatibilidade
Biocompatibility
Bone graft
Bone repair
Enxerto ósseo
Imunoistochemistry
Imunoistoquímica
Osso homógeno
Processo de reparo
LA - pt
PY - 2012
SP - 52-52
ST - Avaliação da incorporação de enxertos ósseos homógenos em humanos: análises
histológica e imunoistoquímica
TI - Avaliação da incorporação de enxertos ósseos homógenos em humanos: análises
histológica e imunoistoquímica
TT - Evaluation of the incorporation of allogenic bone grafts in humans:
histological and immunohistochemical
UR - http://www.athena.biblioteca.unesp.br/exlibris/bd/bfo/33004021011P0/2012/
abla_ms_dr_araca.pdf
ID - 4945
ER -
TY - JOUR
AB - O osso homógeno fresco congelado tem sido utilizado no intuito de substituir
o osso autógeno. No entanto, as suas propriedades de osseoindução e até mesmo
osseocondução não estão bem conceituadas na literatura científica. Esse trabalho
teve por objetivo avaliar amostras de tecido ósseo homógeno enxertados em humanos
através das análises qualitativas histológica e imuno-histoquímica. Para tal, dez
pacientes previamente selecionados foram submetidos à cirurgia de reconstrução de
defeitos ósseos em espessura, usando enxerto ósseo homógeno em bloco fresco
congelado estabilizado e fixado por meio de parafusos bicorticais. Após período de
integração de seis meses foi realizado procedimento de reabertura para instalação
dos implantes osseointegráveis. Neste momento cirúrgico, amostras do enxerto ósseo
foram removidas por meio de broca trefina. As amostras foram fixadas em formol 10%,
levadas ao processamento para corte descalcificado em parafina e coradas por
hematoxilina e eosina. Realizou-se processamento imuno-histoquímico para a
expressão da enzima Caspase 3. As lâminas foram levadas à microscopia óptica para
realização das análises histológica e imuno-histoquímica qualitativas. Os
resultados mostraram tecido ósseo não vital, com poucas áreas de deposição de osso
neoformado sobre a matriz amorfa, presença de infiltrado inflamatório com áreas de
osteomielite e imunomarcação expressiva da enzima Caspase 3. Diante da metodologia
empregada e dos resultados obtidos concluiu-se que o enxerto homógeno fresco
congelado em bloco não incorporou ao leito receptor após período de reparação de
seis meses.
Allogeneic, fresh-frozen bone has been used in order to replace bone autografts.
However, its osteoinduction and osteoconduction properties are not well-defined in
the scientific literature. This work aimed to evaluate samples of homogenous bone
grafts in humans by qualitative histological and immunohistochemical analysis. For
this, ten pre-selected patients underwent surgical augmentation of bone defects.
The homogenous fresh frozen block bone graft was stabilized and fixed by bicortical
screws. After six months, the reopening procedure was performed for installation of
osseointegrated implants. At this time surgical bone graft samples were removed by
means of drill trephine. The samples were fixed in 10% formalin, processed with
decalcified paraffin, and stained with hematoxylin and eosin. Immunohistochemistry
was performed for the expression of Caspase 3 enzyme. The slides were brought to
light microscopy for qualitative histology and immunohistochemistry. The results
showed non-vital bone tissue, with few areas of deposition of new bone formation on
the amorphous matrix, presence of chronic inflammatory infiltrate with areas of
osteomyelitis, and expressive immunolabeling of Caspase 3. Given the methods
employed and the results it was concluded that the allograft fresh-frozen block is
not incorporated into the recipient bed after a healing period of six months
AD - Abla, Marcelo Sabbag
Cetao. BR
Souza, Francisley Ávila
Unesp - Univ Estadual Paulista. Faculdade de Odontologia de Araçatuba. Araçatuba.
BR
Aranega, Alessandra Marcondes
BR
Okamoto, Roberta
Universidade Sagrado Coração USC. BR
Garcia Júnior, Idelmo Rangel
BR
Magro Filho, Osvaldo
BR
AU - Abla, Marcelo Sabbag
AU - Souza, Francisley Ávila
AU - Aranega, Alessandra Marcondes
AU - Okamoto, Roberta
AU - Garcia Júnior, Idelmo Rangel
AU - Magro Filho, Osvaldo
C1 - 20130110
DA - 2012/00
DB - LILACS
IS - 6a
KW - Biocompatibilidade
Enxerto ósseo
Imuno-histoquímica
Osso homógeno
Processo de reparo
LA - pt
PY - 2012
SN - 1678-6661
SP - 159-165
ST - Avaliação da incorporação de enxerto ósseo homógeno fresco congelado em
humanos. Análises histológica e imuno-histoquímica
T2 - ImplantNews
TI - Avaliação da incorporação de enxerto ósseo homógeno fresco congelado em
humanos. Análises histológica e imuno-histoquímica
TT - Evaluation of the allograft fresh frozen incorporation in humans.
Histological and immunohistochemistry analysis
UR - https://pesquisa.bvsalud.org/portal/resource/pt/biblio-851006
VL - 9
ID - 4944
ER -
TY - JOUR
AB - Background > Overcoming the failure percentage of orthodontic mini-screws
(OMSs), which is about 30% of overall orthodontic cases, especially in malocclusion
treatment that requires orthopaedic heavy forces, is a great challenge. Bacterial
infections, soft tissue and bone inflammation, and weak connections between bones
and the OMS surface are among the main causalities of this failure.Objective > The
aim of the study is to evaluate in vitro the microbiological activities of the
deposited nanomaterials (Silver/hydroxyapatite nanoparticles (Ag/HA NPs) and zinc
oxide nanoparticles (ZnO NPs)) in terms of microbial inhibition. In addition, the
in-vitro cytotoxicity and cytocompatibility of the synthesized nano-coatings prior
to their in-vivo application in animal models were tested on four types of cells,
namely, fibroblasts, osteocytes, osteoblasts, and oral epithelial cells.Materials
and methods > Ag/HA NPs and ZnO NPs were built up onto the surface of titanium OMSs
by electrochemical deposition. This electrochemical deposition was performed on 50
orthodontic mini screws and the deposited materials were characterized with the aid
of scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX)
analysis, X-ray Diffraction (XRD) and nano -scratch test. In addition, the
microbiological activities of the deposited nanomaterials were explored in vitro in
terms of microbial inhibition. Furthermore, the cytotoxicity and cytocompatibility
were tested on four types of cells, namely, fibroblasts, osteocytes, osteoblasts
and oral epithelial cells.Results > SEM images revealed spherical Ag NPs in the
range of 40-70 nm in diameter, rod-shaped HA NPs and porous scaly ZnO NPs on the
surface of the OMSs. XRD analysis confirmed the crystal structures of AgNPs, HA
NPs, and ZnO NPs. ZnO NPs coated OMS had the highest antimicrobial activity than
Ag/ HA coated OMS against Gram-positive, Gram-negative and fungal strains.
Moreover, after incubation, the decrease in the number of bacterial colonies was
significant with ZnO and Ag/HA nanoparticles (with the greatest decrease for the
former), due to the potent antibacterial effect of nanoparticles against
Escherichia coli and Enterococcus faecalis. Moreover, ZnO NPs-coated OMSs showed a
better cytocompatibility with oral epithelium, bone cells, and fibroblasts compared
to Ag/HA NPs. Conclusion > The suggested nanocoating is a promising strategy to
overcome the development of an inflammatory zone around the fixed OMSs.
AN - WOS:000903532000007
AU - Abo-Elmahasen, M. M. F.
AU - Dena, A. S. A.
AU - Zhran, M.
AU - Albohy, S. A. H.
C6 - DEC 2022
DA - MAR
DO - 10.1016/j.ortho.2022.100711
IS - 1
PY - 2023
SN - 1761-7227
1879-680X
ST - Do silver/hydroxyapatite and zinc oxide nano-coatings improve inflammation
around titanium orthodontic mini-screws? In vitro study
T2 - INTERNATIONAL ORTHODONTICS
TI - Do silver/hydroxyapatite and zinc oxide nano-coatings improve inflammation
VL - 21
ID - 6310
ER -
TY - JOUR
AB - Simple Summary The main objective of this study is to assess how eugenol
(Eug) affects AgNP-induced nephrotoxicity in rats. After 30 days of treatment with
AgNPs, rats developed nephrotoxicity, which was characterized by disruptions in the
serum levels of blood urea nitrogen, creatinine, uric acid, the total oxidant
capacity, the total antioxidant capacity, and interfering levels of kidney injury
molecule-1, superoxide dismutase, catalase, reduced glutathione, glutathione
peroxidase, malondialdehyde, tumor necrosis factor-alpha (TNF-alpha), and
interleukin-6 in kidney tissues. These biochemical alterations were accompanied by
the destruction of normal renal architecture, with most renal components shedding
their thickness, diameter, and quantity. Furthermore, P53, Caspase3, and TNF-alpha
immunoreactivity were considerably elevated; however, Bcl-2 immunoreactivity was
reduced. Most biochemical, histological, histomorphometrical, and
immunohistochemical alterations in AgNP-treated rats were reversed by Eug. We may
infer that Eug has a protective effect against AgNP-induced nephrotoxicity. The use
of silver nanoparticles (AgNPs) is expanding. This study evaluates the modulator
effect of eugenol (Eug) on AgNP-induced nephrotoxicity in rats. Sixty male rats
were separated into six groups: control, Eug, AgNPs low-dose, AgNPs high-dose, Eug
+ AgNPs low-dose, and Eug + AgNPs high-dose. After 30 days, kidney function,
antioxidative and proinflammatory status, histopathological, histomorphometrical,
and immunohistochemical assessments were performed. AgNPs markedly induced
oxidative stress in renal tissues, characterized by increased levels of blood urea
nitrogen, creatinine, uric acid, kidney injury molecule-1, the total oxidant
capacity, malondialdehyde, tumor necrosis factor-alpha (TNF-alpha), and
interleukin-6, as well as decreased levels of the total antioxidant capacity,
superoxide dismutase, catalase, reduced glutathione, and glutathione peroxidase.
Moreover, the normal renal architecture was destroyed, and the thickness of the
renal capsules, cortex, and medulla, alongside the diameter and quantity of the
normal Malpighian corpuscles and the proximal and distal convoluted tubules were
decreased. Immunoreactivity for P53, caspase-3, and TNF-alpha reactive proteins
were significantly increased; however, Bcl-2 immunoreactivity was decreased. Eug
reversed most biochemical, histological, histomorphometrical, and
immunohistochemical changes in AgNP-treated animals. This study demonstrated that
nephrotoxicity in AgNP-treated rats was mitigated by an Eug supplementation. Eug's
antioxidant, antiapoptotic, and anti-inflammatory capabilities were the key in
modulating AgNPs nephrotoxicity.
AN - WOS:000902360300001
AU - Aboelwafa, H. R.
AU - Ramadan, R. A.
AU - Ibraheim, S. S.
AU - Yousef, H. N.
C7 - 1719
DA - DEC
DO - 10.3390/biology11121719
IS - 12
PY - 2022
SN - 2079-7737
ST - Modulation Effects of Eugenol on Nephrotoxicity Triggered by Silver
Nanoparticles in Adult Rats
T2 - BIOLOGY-BASEL
TI - Modulation Effects of Eugenol on Nephrotoxicity Triggered by Silver
Nanoparticles in Adult Rats
VL - 11
ID - 6011
ER -
TY - CHAP
AB - Zika virus (ZIKV) specifically infects cells in the brain cortex, but it is
unclear if astrocytes, brain microvascular endothelial cells, neurons, pericytes,
and microglia can be infected by ZIKV. Additionally, the mechanisms that underlie
the neuropathogenic effects of the ZIKV remain to be determined. ZIKV crosses the
blood-brain barrier (BBB), which led us to examine the potential neurotoxic effects
on microglia, which are the immune cells of the CNS. The current study examined the
ability of ZIKV to infect HBMVEC, normal human astrocyte (NHA) and human microglia
cells, and the effects of ZIKV on the expression of inflammatory cytokines and the
immunomodulatory and antiviral effects of a curcumin-based silver nanoparticle
(Cur-AgNP) nanoformulation on ZIKV-infected microglial cells in vitro.
Additionally, our study examined the potential mechanisms that may underlie
neuroinflammation associated with ZIKV infection that contributes to ZIKV-
associated neuropathology. Our results indicate that the microglial cells are most
susceptible to the ZIKV and the treatment of microglial cultures with the ZIKV
resulted in a significant increase in inflammatory response as evident by the
increased expression of proinflammatory cytokines. Further, the Cur-AgNP
nanoformulation significantly reduced ZIKV expression in ZIKV-infected microglia
and inhibited the expression of cytokines such as, IL-4, INF-γ, and IL-1β. The ZIKV
activity was associated with increased apoptosis and oxidative stress, and
treatment with Cur-AgNP reduced microglial apoptosis and decreased both reactive
oxygen and nitric oxide species, thereby decreasing neuroinflammatory response. The
Cur-AgNP nanoformulation could thus have clinical utility as a potential antiviral
therapeutic in neuroinflammation associated with ZIKV infection. © 2022 Elsevier
Inc. All rights reserved.
AU - Abou-Jaoude, M.
AU - Sharma, R. K.
AU - Nair, A.
AU - Mammen, M. J.
AU - Aalinkeel, R.
AU - Schwartz, S. A.
AU - Mahajan, S. D.
DB - Scopus
DO - 10.1016/B978-0-323-99596-2.00013-3
KW - AgNPs
Blood-brain barrier
Curcumin
Cytokines
Immunomodulation
Nanoformulation
Reactive oxygen species
Zika virus
PY - 2022
SP - 113-128
ST - Nanotherapy approach to target ZIKA virus in microglia: A case study
T2 - Nanotechnological Applications in Virology
TI - Nanotherapy approach to target ZIKA virus in microglia: A case study
85137572518&doi=10.1016%2fB978-0-323-99596-2.00013-
3&partnerID=40&md5=dc3d685fb598f05274d1ffd23ab39a6f
ID - 5119
ER -
TY - JOUR
AB - Objective: Egyptian Purslane (Portulaca oleracea) is rich in omega-3 fatty
acids and a wide range of vitamins and phyto-constituents that were absorbed slowly
due to their high molecular weights. Therefore, this study was designed to
accelerate the absorption of these phyto-constituents and hence increase their
bioavailability by incorporating silver (Ag-NPs) and zinc oxide nanoparticles (ZnO-
NPs) due to their impressive properties. Methods: The major phytoconstituents and
different biological activities were quantified in aqueous extract before and after
incorporating metal nanoparticles (M-NPs). The efficiency of ZnO-P. nano-extract
was studied on cell cycle and apoptosis of human hepatocellular carcinoma (HEPG-2)
cells. Then, both Ag and ZnO-P. nano-extracts were studied against hepatic fibrosis
induced by thioacetamide (TAA) in rats through undergoing different hematological
and biochemical measurements in addition to the histopathological examination in
hepatic tissues compared to the extract itself. Results: The ZnO-P. nano-extract
showed significantly (P<0.05) higher antioxidant and scavenging activity due to the
existence of higher total polyphenolic content. Also, it exhibited a significantly
(P<0.05) higher inhibitory effect on acetyl cholinesterase (AChE) activity and
higher cytotoxic activity against HEPG-2 cells. Therefore, ZnO-P. nano-extract was
studied against the cell cycle and apoptosis of HEPG-2 cells compared to the
extract itself. It was found that ZnO-P. nano-extract was safer than Ag-P. nano-
extract. Both Ag and ZnO-P. nano-extracts were studied against the hepatic fibrosis
induced by thioacetamide (TAA) compared to the native extract. It was noticed that
ZnO-P. nano-extract exhibited an ameliorative effect against hepatic fibrosis by
decreasing levels of inflammatory and fibrotic markers significantly (P<0.05) more
than Ag-P. nano-extract. Furthermore, it improved the antioxidant status of the
hepatic tissue in addition to restoring the histopathological architecture of liver
tissue. Conclusion: ZnO-P. nano-extract showed higher in vitro and in vivo
biological activities than Ag-P. nano-extract and native P. extract itself ©
2022,Asian Pacific Journal of Cancer Prevention.All Rights Reserved
AU - Aboulthana, W. M.
AU - Omar, N. I.
AU - Hasan, E. A.
AU - Ahmed, K. A.
AU - Youssef, A. M.
DB - Scopus
DO - 10.31557/APJCP.2022.23.1.287
IS - 1
KW - Biological activity
Green nanotechnology
Portulaca oleracea
Zinc oxide nanoparticles
Animals
Cell Line, Tumor
Disease Models, Animal
Hep G2 Cells
Humans
Liver Cirrhosis
Metal Nanoparticles
Plant Extracts
Portulaca
Rats
Silver
Thioacetamide
Zinc Oxide
metal nanoparticle
plant extract
silver
thioacetamide
zinc oxide
animal
disease model
Hep-G2 cell line
human
liver cirrhosis
rat
tumor cell line
M3 - Article
PY - 2022
SP - 287-310
ST - Assessment of the Biological Activities of Egyptian Purslane (Portulaca
oleracea) Extract after Incorporating Metal Nanoparticles, in Vitro and in Vivo
Study
T2 - Asian Pacific Journal of Cancer Prevention
TI - Assessment of the Biological Activities of Egyptian Purslane (Portulaca
oleracea) Extract after Incorporating Metal Nanoparticles, in Vitro and in Vivo
Study
85123877576&doi=10.31557%2fAPJCP.2022.23.1.287&partnerID=40&md5=c7f83bd67f16ffed293
086a876090ad2
VL - 23
ID - 5131
ER -
TY - JOUR
AB - The synthesis of reliable biological nanomaterials is a crucial area of study
in nanotechnology. In this study, Emericella dentata was employed for the
biosynthesis of AgNPs, which were then combined with synthesized biochar, a porous
structure created through biomass pyrolysis. The synergistic effects of AgNPs and
biochar were evaluated through the assessment of pro-inflammatory cytokines, anti-
apoptotic gene expression, and antibacterial activity. Solid biosynthesized AgNPs
were evaluated by XRD and SEM, with SEM images revealing that most of the AgNPs
ranged from 10 to 80 nm, with over 70% being less than 40 nm. FTIR analysis
indicated the presence of stabilizing and reducing functional groups in the AgNPs.
The nanoemulsion's zeta potential, hydrodynamic diameter, and particle distribution
index were found to be -19.6 mV, 37.62 nm, and 0.231, respectively. Biochar, on the
other hand, did not have any antibacterial effects on the tested bacterial species.
However, when combined with AgNPs, its antibacterial efficacy against all bacterial
species was significantly enhanced. Furthermore, the combined material
significantly reduced the expression of anti-apoptotic genes and pro-inflammatory
cytokines compared to individual treatments. This study suggests that low-dose
AgNPs coupled with biochar could be a more effective method to combat lung cancer
epithelial cells and pathogenic bacteria compared to either substance alone.
AN - WOS:001017193200001
AU - Abu Hajleh, M. N.
AU - Al-limoun, M.
AU - Al-Tarawneh, A.
AU - Hijazin, T. J.
AU - Alqaraleh, M.
AU - Khleifat, K.
AU - Al-Madanat, O. Y.
AU - Al Qaisi, Y.
AU - AlSarayreh, A.
AU - Al-Samydai, A.
AU - Qaralleh, H.
AU - Al-Dujaili, E. A. S.
C7 - 4757
DA - JUN
DO - 10.3390/molecules28124757
IS - 12
PY - 2023
SN - 1420-3049
ST - Synergistic Effects of AgNPs and Biochar: A Potential Combination for
Combating Lung Cancer and Pathogenic Bacteria
T2 - MOLECULES
TI - Synergistic Effects of AgNPs and Biochar: A Potential Combination for
Combating Lung Cancer and Pathogenic Bacteria
VL - 28
ID - 6426
ER -
TY - JOUR
AB - Despite the potential antimicrobial and water purification benefits of
chitosan-based nanocomposites, there are growing concerns regarding the hazards of
leached nanoparticles (NPs) to the in-contact circumference. The antibacterial
performance of the nanocomposites of chitosan with silver and copper NPs and carbon
nanotubes was assessed with an emphasis on their impact on fish health. The minimal
inhibitory concentrations of each preparation and the growth curves of Aeromonas
hydrophila exposed to different nanocomposites were measured. Five groups of
Oreochromis niloticus were exposed to chitosan nanocomposites for three weeks. A
combination of a low concentration of the NPs in the chitosan matrix improved their
antimicrobial properties. However, aqueous exposure to these materials still had
hazardous effects on fish health. Experimental groups of O. niloticus exposed to
these nanocomposites exhibited oxidative stress, tissue DNA fragmentation and
higher expression of pro-inflammatory and immune-related genes such as TNF-α and
IL1β. Various pathological tissue alterations were observed in gills, liver, spleen
and intestine. Exposure to some of the prepared nanocomposites led to significant
DNA damage in hepatic cells with a marked increase in the apoptotic index. © 2018
Elsevier B.V.
AU - Abu-Elala, N. M.
AU - AbuBakr, H. O.
AU - Khattab, M. S.
AU - Mohamed, S. H.
AU - El-hady, M. A.
AU - Ghandour, R. A.
AU - Morsi, R. E.
DB - Scopus
DO - 10.1016/j.ijbiomac.2018.03.047
KW - Antimicrobial agents
Chitosan nanocomposites
Environmental risk assessment
Aeromonas hydrophila
Animals
Anti-Infective Agents
Aquatic Organisms
Chitosan
Cichlids
Copper
Cytokines
Dose-Response Relationship, Drug
Ecotoxicology
Gene Expression Regulation
Microbial Sensitivity Tests
Nanocomposites
Nanotubes, Carbon
Oxidative Stress
Risk Assessment
Silver
antiinfective agent
carbon nanotube
chitosan
copper nanoparticle
glutathione
interleukin 1beta
malonaldehyde
nanocomposite
silver nanoparticle
copper
cytokine
silver
animal cell
animal experiment
animal health
animal tissue
apoptosis
apoptotic index
aquatic environment
Article
bacterial growth
cell infiltration
controlled study
disease severity
DNA fragmentation
exposure
gene expression
health hazard
IL 1beta gene
lipid peroxidation
liver cell
nonhuman
oxidative stress
particle size
risk assessment
tissue injury
TNF alpha gene
animal
aquatic species
chemistry
cichlid
dose response
drug effect
ecotoxicology
growth, development and aging
metabolism
microbial sensitivity test
M3 - Article
PY - 2018
SP - 1105-1115
ST - Aquatic environmental risk assessment of chitosan/silver, copper and carbon
nanotube nanocomposites as antimicrobial agents
T2 - International Journal of Biological Macromolecules
TI - Aquatic environmental risk assessment of chitosan/silver, copper and carbon
nanotube nanocomposites as antimicrobial agents
85043995780&doi=10.1016%2fj.ijbiomac.2018.03.047&partnerID=40&md5=8ed5e132510e5f19b
28e26ad67cc69e9
VL - 113
ID - 5490
ER -
TY - JOUR
AB - Intrapleural talc is used to produce pleurodesis in malignant pleural
effusions. Prior in vivo studies have documented an acute inflammatory response to
talc in the pleural space but the cellular source of cytokines has not been
identified. The aim of this study was to investigate the acute response of rabbit
pleural mesothelial cells challenged with talc used for pleurodesis and compare it
to prior studies of the response to talc in the rabbit pleural space. Cultured
rabbit pleural mesothelial cells (PMC) were exposed to talc (25 mu g/cm(2)) for 6,
24, or 48 h and assessed for viability, necrosis, and apoptosis by flow cytometry,
Trypan Blue exclusion, and immunocytochemistry, and for the production of
interleukin-8 (IL-8), vascular endothelial growth factor (VEGF), and transforming
growth factor-beta(1) (TGF-beta(1)) by ELISA. More than 50% of the PMC remained
viable 48 h after talc stimulation. The PMC that were nonviable were identified as
either apoptotic or necrotic, with roughly 20% in each category over the 48 h. At 6
h, the IL-8, VEGF, and TGF-beta(1) levels produced by talc-exposed PMC increased
significantly and remained elevated for up to 48 h. These cytokine levels rose at
similar times and at the same or higher levels than have been measured in the
rabbit pleural space in prior studies. We report that viable, talc-exposed, pleural
mesothelial cells may actively mediate the primary inflammatory pleural response in
talc-induced pleurodesis.
AN - WOS:000251000200005
AU - Acencio, M. M. P.
AU - Vargas, F. S.
AU - Marchi, E.
AU - Carnevale, G. G.
AU - Teixeira, L. R.
AU - Antonangelo, L.
AU - Broaddus, V. C.
DA - DEC
DO - 10.1007/s00408-007-9041-y
IS - 6
PY - 2007
SN - 0341-2040
1432-1750
SP - 343-348
ST - Pleural mesothelial cells mediate inflammatory and profibrotic responses in
talc-induced pleurodesis
T2 - LUNG
TI - Pleural mesothelial cells mediate inflammatory and profibrotic responses in
talc-induced pleurodesis
VL - 185
ID - 6239
ER -
TY - JOUR
AB - Introduction: Polymethylmethacrylate (PMMA) acrylic resins are used to make
dentures for edentulous patients. Objective: To find out the prevalence of Candida
species in patients with and without removable prostheses from a dental clinic in
León, Gua-najuato, as well as to assess the antifungal effect and biological
behavior of an experimental PMMA with silver nanoparticles for its possible
application in prostheses. Method: To identify Candida species, smear samples were
obtained from the palatal mucosa of 140 patients aged ≥ 60 years. The experimental
PMMA with silver nanoparticles was placed in Candida albicans cultures, which were
stained with the Live/Dead® kit for analysis under confocal microscopy;
subsequently, it was implanted in Wistar rats in order to know its behavior in the
surrounding tissues. Results: Candida albicans was the most prevalent species in
the evaluated patients, followed by Candida tropicalis and Candida krusei. The
acrylic resin with silver nanoparticles significantly decreased the presence of
Candida albicans. In the animal model, a discrete and controlled inflammatory
reaction was found, which indicated biocompatibility of the acrylic resin that was
used. Conclusions: It is possible for the nanostructured material with antifungal
effect to be used in order to promote the reduction of oral Candida infections in
edentulous patients. © 2021, Academia Nacional de Medicina. All rights reserved.
AU - Acosta-Torres, L. S.
AU - Flores-Arriaga, J. C.
AU - Serrano-Díaz, P. N.
AU - González-García, I. A.
AU - Viveros-García, J. C.
AU - Villanueva-Vilchis, M. C.
AU - Villanueva-Sánchez, F. G.
AU - García-Contreras, R.
AU - Arenas-Arrocena, M. C.
DB - Scopus
DO - 10.24875/GMM.20000915
IS - 4
KW - Acrylic resin
Antifungal
Biocompatibility in animal model
antifungal agent
biomaterial
poly(methyl methacrylate)
silver nanoparticle
adult
animal experiment
animal model
animal tissue
antifungal activity
Article
biocompatibility
buccal mucosa
Candida albicans
confocal microscopy
controlled study
dental clinic
edentulousness
human
inflammation
major clinical study
male
middle aged
nonhuman
rat
M3 - Article
PY - 2021
SP - 437-442
ST - Biomaterial antifúngico para reducir las infecciones causadas por candida
albicans en pacientes edéntulos
T2 - Gaceta Medica de Mexico
TI - Antifungal biomaterial for reducing infections caused by candida albicans in
edentulous patients
85121732842&doi=10.24875%2fGMM.20000915&partnerID=40&md5=362d51a5c2e54d89201c468f5a
2f6513
VL - 157
ID - 5158
ER -
TY - JOUR
AB - INTRODUCTION: Polymethylmethacrylate (PMMA) acrylic resins are used to make
dentures for edentulous patients. OBJECTIVE: To find out the prevalence of Candida
León, Guanajuato, as well as to assess the antifungal effect and biological
application in prostheses. METHOD: To identify Candida species, smear samples were
obtained from the palatal mucosa of 140 patients aged ≥ 60 years. The experimental
PMMA with silver nnoparticles was placed in Candida albicans cultures, which were
stained with the Live/Dead® kit for analysis under confocal microscopy;
surrounding tissues. RESULTS: Candida albicans was the most prevalent species in
used. CONCLUSIONS: It is possible for the nanostructured material with antifungal
edentulous patients. Copyright: © 2021 Permanyer.; INTRODUCCIÓN: Las resinas
acrílicas de polimetilmetacrilato (PMMA) son utilizadas para elaborar dentaduras
para pacientes edéntulos. OBJETIVO: Conocer la prevalencia de las especies de
Candida en pacientes con y sin prótesis removibles de una clínica de odontología en
León, Guanajuato; así como valorar el efecto antifúngico y el comportamiento
biológico de un PMMA experimental con nanopartículas de plata para su posible
aplicación en prótesis. MÉTODO: Para identificar las especies de Candida se
obtuvieron muestras para frotis de la mucosa palatina de 140 pacientes con edad ≥
60 años. El PMMA experimental con nanopartículas de plata fue colocado en cultivos
de Candida albicans, los cuales fueron teñidos con el kit Live/Dead® para su
análisis bajo microscopia confocal; posteriormente, se implantó en ratas Wistar
para conocer su comportamiento en los tejidos circundantes. RESULTADOS: Candida
albicans fue la especie más prevalente en los pacientes valorados, seguida de
Candida tropicalis y Candida krusei. La resina acrílica con nanopartículas de plata
disminuyó significativamente la presencia de Candida albicans. En el modelo animal
se encontró reacción inflamatoria discreta y controlada, lo cual indicó la
biocompatibilidad de la resina acrílica utilizada. CONCLUSIONES: Es posible
utilizar el material nanoestructurado con efecto antifúngico para promover la
reducción de infecciones orales por Candida en pacientes edéntulos.
AU - Serrano-Díaz, P. N.
AU - González-García, I. A.
AU - Viveros-García, J. C.
AU - Villanueva-Vilchis, M. D. C.
AU - Villanueva-Sánchez, F. G.
AU - García-Contreras, R.
DB - Scopus
DO - 10.24875/GMM.M21000585
IS - 4
KW - Acrylic resin
Antifungal
Antifúngico
Biocompatibilidad en modelo animal
Biocompatibility in animal model
Nanopartículas de plata
Resina acrílica
Animals
Antifungal Agents
Biocompatible Materials
Candida albicans
Humans
Metal Nanoparticles
Rats
Rats, Wistar
Silver
antifungal agent
biomaterial
metal nanoparticle
silver
animal
human
rat
Wistar rat
M3 - Article
PY - 2021
SP - 422-427
ST - Biomaterial antifúngico para reducir las infecciones causadas por Candida
albicans en pacientes edéntulos
T2 - Gaceta medica de Mexico
TI - Antifungal biomaterial for reducing infections caused by Candida albicans in
edentulous patients
85124321924&doi=10.24875%2fGMM.M21000585&partnerID=40&md5=a4baf23c044d2cc474cfb076c
b44c733
VL - 157
ID - 5178
ER -
TY - JOUR
AB - Introduction: Polymethylmethadylate (PMMA) acrylic resins are used to make
dentures for edentulous patients. Objective: To find out the prevalence of Candida
Leon, Guanajuato, as well as to assess the antifungal effect and biological
application in prostheses. Method: To identify Candida species, smear samples were
obtained from the palatal mucosa of 140 patients aged 60 years. The experimental
PMMA with silver nanoparticles was placed in Candida albicans cultures, which were
stained with the Live/Deae kit for analysis under confocal microscopy;
surrounding tissues. Results: Candida albicans was the most prevalent species in
used. Conclusions: It is possible for the nanostructured material with antifungal
edentulous patients.
AN - WOS:000721676900015
AU - Serrano-Diaz, P. N.
AU - Gonzalez-Garcia, I. A.
AU - Viveros-Garcia, J. C.
AU - Villanueva-Vilchis, M. D.
AU - Villanueva-Sanchez, F. G.
AU - Garcia-Contreras, R.
DA - JUL-AUG
DO - 10.24875/GMM.20000915
IS - 4
PY - 2021
SN - 0016-3813
SP - 437-442
ST - Antifungal biomaterial for reducing infections caused by Candida albicans in
edentulous patients
T2 - GACETA MEDICA DE MEXICO
TI - Antifungal biomaterial for reducing infections caused by Candida albicans in
edentulous patients
VL - 157
ID - 6139
ER -
TY - JOUR
AB - Ethnopharmacological relevance: Sphenocentrum jollyanum is a flowering plant
of the Menispermaceae family with bright yellow roots and wedged-shaped leaves. The
plant is reputed to possess exceptional wound healing properties and used in
folkloric medicine to dress chronic wounds. Aim of the study: Wound repair in a
hyperglycemic state is known to be impaired and delayed making treatment a
difficult challenge. This study sought how the aqueous extracts of root and leaf of
Sphenocentrum jollyanum facilitated wound healing by modulating pro-inflammatory
cytokines, vascular endothelial growth factor and microbial colonization on
excision wound created in diabetic rats. Methods: Diabetes (blood glucose >250
mg/dl) was induced by feeding normal rats with high fat diet for 14 days after
which intraperitoneal injection of low dose streptozotocin (35 mg/kg b.w.) was
administered. Wounds were subsequently created and treatments administered
afterwards for 14 days. Results: Administration of Sphenocentrum jollyanum root and
leaf extracts both orally and topically (100 and 200 mg/kg b.w) significantly (p <
0.05) reduced secretion of pro-inflammatory cytokines (TNF-α, IL-6), number of
microbial colonies (CFU/ml × 102), activity of myeloperoxidase and significantly
increased growth factor secretion on wounds of the diabetic rats. Histological
evaluations of wound tissues of treated diabetic rats revealed matured tissue
granulation, presence of new blood vessels, collagen and fibroblast with fewer
inflammatory cells. Conclusion: The use of Sphenocentrum jollyanum effectively
enhanced wound healing which may be related to constituents identified by GC-MS
analysis and can thus, be suggested as a therapeutic agent for diabetic wound
management. © 2022 Elsevier B.V.
AU - Adeleke, O.
AU - Oboh, G.
AU - Adefegha, S.
AU - Osesusi, A.
C7 - 115266
DB - Scopus
DO - 10.1016/j.jep.2022.115266
KW - Angiogenesis
Cytokine
Growth factor
Microbes
Sphenocentrum jollyanum
Wound healing
Animals
Cytokines
Diabetes Mellitus, Experimental
Menispermaceae
Plant Extracts
Rats
Vascular Endothelial Growth Factor A
1 methyl 4 [nitromethyl] 4 piperidinol
4 amino 2 chloropyridine
4 bromo n (piperidinomethyl)phthalimide
antibiotic agent
benzopyrazine(quinoxaline)
chloramphenicol
collagen
cytokine
glucose
interleukin 6
malonaldehyde
myeloperoxidase
plant extract
pyridinemethanol
pyrrolo[3,4 c]pyridine 1,3 dione
RNA
Sphenocentrum jollyanum extract
streptozocin
sulfadiazine silver
unclassified drug
uracil
urea
uric acid
vasculotropin
wound healing promoting agent
vasculotropin A
acute toxicity
animal cell
animal experiment
animal model
animal tissue
Article
bacterial count
controlled study
diabetic patient
diabetic wound
fibroblast
glucose blood level
granulation tissue
in vivo study
inflammatory cell
lipid diet
male
mass fragmentography
microbial colonization
nonhuman
pathogen load
plant leaf
plant root
rat
RNA isolation
single drug dose
streptozotocin-induced diabetes mellitus
topical treatment
wound closure
wound healing
wound tissue
animal
experimental diabetes mellitus
metabolism
M3 - Article
PY - 2022
ST - Effect of aqueous extract from root and leaf of Sphenocentrum jollyanum
pierre on wounds of diabetic rats: Influence on wound tissue cytokines, vascular
endothelial growth factor and microbes
T2 - Journal of Ethnopharmacology
TI - Effect of aqueous extract from root and leaf of Sphenocentrum jollyanum
pierre on wounds of diabetic rats: Influence on wound tissue cytokines, vascular
endothelial growth factor and microbes
85128566858&doi=10.1016%2fj.jep.2022.115266&partnerID=40&md5=52064f67bbb668f1abb39e
cd3bcd0b1f
VL - 293
ID - 5139
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) synthesized from plant extracts are widely used
for the cure of many diseases from fever to cancers. Keeping in view the medicinal
value of AgNPs, here we report a cost-effective phytochemical method for the
biosynthesis of silver nanoparticles from Ajuga bracteosa. A. bracteosa is an
important medicinal plant used to cure fever, appetite-loss, and cancer. Silver-
nanoparticles were prepared from the aqueous extract of the plant. The methanolic
extract of A. bracteosa (ABMF) was separated and n-hexane (ABHF) and chloroform
(ABCF) fractions were obtained from the methanolic crude extract. The AgNPs were
characterized by UV-Visible spectrophotometer, FTIR, XRD, and TEM. The total
phenolic contents (TPC) and total flavonoid contents (TFC) in different fractions
were determined and compared with AgNPs. The medicinal value of ABMF, ABHF, ABCF,
and AgNPs was evaluated by antibacterial, antioxidant, anti-inflammatory, and
cytotoxicity bioassays. The UV-visible spectrum showed a peak at 484 nm while FTIR
results suggested strong capping of phytochemicals on AgNPs which was confirmed by
a high amount of TPC and TFC. XRD analysis depicted a high degree of crystallinity
and smaller size of AgNPs. TEM results showed spherical shaped AgNPs of size range
50 12 nm. The biosynthesized AgNPs showed better antibacterial activity than plant
extract fractions. Similarly, AgNPs have shown better antioxidant, cytotoxicity
against cancer cell lines in-vitro, and anti-inflammatory activity in-vivo than a
plant extract. The great medicinal value of A. bracteosa might be due to the
presence of pharmacologically active phytochemicals such as diterpenoids, neo-
clerodane flavonol glycosides, ergosterol, iridoid glycosides, phytoecdysones, and
other polyphenols. These phytochemicals surround the silver nanoparticles during
green synthesis and therefore, this capping of phytochemicals over silver
nanoparticles results in enhanced biomedical applications of plant extracts. © 2020
The Author(s). Published by IOP Publishing Ltd.
AU - Afreen, A.
AU - Ahmed, R.
AU - Mehboob, S.
AU - Tariq, M.
AU - Alghamdi, H. A.
AU - Zahid, A. A.
AU - Ali, I.
AU - Malik, K.
AU - Hasan, A.
C7 - 075404
DB - Scopus
DO - 10.1088/2053-1591/aba5d0
IS - 7
KW - antibacterial
antioxidant
cytotoxicity
phytochemicals
Antioxidants
Biochemistry
Biosynthesis
Cell culture
Chlorine compounds
Cost effectiveness
Crystallinity
Curing
Diseases
Green Synthesis
Hexane
Medical applications
Metal nanoparticles
Sugars
X ray diffraction
Anti-bacterial activity
Anti-inflammatory activity
Biomedical applications
Degree of crystallinity
Silver nanoparticles (AgNps)
Total flavonoid contents
Total phenolic content
UV-visible spectrophotometer
Plant extracts
M3 - Article
PY - 2020
ST - Phytochemical-assisted biosynthesis of silver nanoparticles from Ajuga
bracteosa for biomedical applications
T2 - Materials Research Express
TI - Phytochemical-assisted biosynthesis of silver nanoparticles from Ajuga
bracteosa for biomedical applications
85088977854&doi=10.1088%2f2053-
1591%2faba5d0&partnerID=40&md5=de192ca948029ee5f738860ec75a7355
VL - 7
ID - 5340
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) synthesized from plant extracts are widely used
for the cure of many diseases from fever to cancers. Keeping in view the medicinal
value of AgNPs, here we report a cost-effective phytochemical method for the
biosynthesis of silver nanoparticles fromAjuga bracteosa.A. bracteosais an
important medicinal plant used to cure fever, appetite-loss, and cancer. Silver-
nanoparticles were prepared from the aqueous extract of the plant. The methanolic
extract ofA. bracteosa(ABMF) was separated and n-hexane (ABHF) and chloroform
(ABCF) fractions were obtained from the methanolic crude extract. The AgNPs were
characterized by UV-Visible spectrophotometer, FTIR, XRD, and TEM. The total
phenolic contents (TPC) and total flavonoid contents (TFC) in different fractions
were determined and compared with AgNPs. The medicinal value of ABMF, ABHF, ABCF,
and AgNPs was evaluated by antibacterial, antioxidant, anti-inflammatory, and
cytotoxicity bioassays. The UV-visible spectrum showed a peak at 484 nm while FTIR
results suggested strong capping of phytochemicals on AgNPs which was confirmed by
a high amount of TPC and TFC. XRD analysis depicted a high degree of crystallinity
and smaller size of AgNPs. TEM results showed spherical shaped AgNPs of size range
50 12 nm. The biosynthesized AgNPs showed better antibacterial activity than plant
extract fractions. Similarly, AgNPs have shown better antioxidant, cytotoxicity
against cancer cell linesin-vitro,and anti-inflammatory activityin-vivothan a plant
extract. The great medicinal value ofA. bracteosamight be due to the presence of
pharmacologically active phytochemicals such as diterpenoids, neo-clerodane
flavonol glycosides, ergosterol, iridoid glycosides, phytoecdysones, and other
polyphenols. These phytochemicals surround the silver nanoparticles during green
synthesis and therefore, this capping of phytochemicals over silver nanoparticles
results in enhanced biomedical applications of plant extracts.
AN - WOS:000555559900001
AU - Afreen, A.
AU - Ahmed, R.
AU - Mehboob, S.
AU - Tariq, M.
AU - Alghamdi, H. A.
AU - Zahid, A. A.
AU - Ali, I.
AU - Malik, K.
AU - Hasan, A.
C7 - 075404
DA - JUL
DO - 10.1088/2053-1591/aba5d0
IS - 7
PY - 2020
SN - 2053-1591
ST - Phytochemical-assisted biosynthesis of silver nanoparticles fromAjuga
bracteosafor biomedical applications
T2 - MATERIALS RESEARCH EXPRESS
TI - Phytochemical-assisted biosynthesis of silver nanoparticles fromAjuga
bracteosafor biomedical applications
VL - 7
ID - 5862
ER -
TY - JOUR
AB - Apoptotic cell death occurs frequently after cerebral ischemia, although
prevailing necrosis could also be observed in older animals. The mechanisms that
lead to delayed cell death after hypoxic-ischemic injury in the developing brain
are not yet known. With silver staining, we showed increased dying neurons after
ischemia and 4 to 24 hours of reperfusion in 7-day-old rats. Using electron
microscopic studies, we investigated the presence of chromatin condensation.
Injured neurons exhibited 2 different morphological types of apoptosis: Type 1
(aggregation of dense masses of chromatin beneath the intact nuclear membrane)
predominately, and Type 2 (cytoplasmic vacuolization). Electron microscopy analysis
also showed that the fragmented chromatin and neuronal debris were phagocytosed by
surrounding glial cells, and associated with an inflammatory reaction leading to
leukocyte infiltration. These results suggest that the immature brain may be more
prone to apoptotic death with neuronal loss by neighboring cells. This experimental
model may be useful for identifying the biochemical mechanisms that initiate and
mediate Type 1 neuronal apoptosis after ischemia and reperfusion in neonate
rodents.
AU - Aggoun-Zouaoui, D.
AU - Ben-Ari, Y.
AU - Charriaut-Marlangue, C.
DB - Scopus
DO - 10.1016/S1052-3057(00)07279-7
IS - 1
KW - Cell death
Glial cells
Mitosis
Neuron
animal cell
apoptosis
article
brain hypoxia
brain ischemia
brain maturation
chromatin condensation
female
glia cell
male
nerve cell lesion
nerve cell necrosis
newborn
nonhuman
priority journal
rat
reperfusion
M3 - Article
PY - 2000
SP - 8-15
ST - Neuronal type 1 apoptosis after unilateral focal ischemia with reperfusion in
the P7 neonatal rat
T2 - Journal of Stroke and Cerebrovascular Diseases
TI - Neuronal type 1 apoptosis after unilateral focal ischemia with reperfusion in
the P7 neonatal rat
0033759887&doi=10.1016%2fS1052-3057%2800%2907279-
7&partnerID=40&md5=5bbc09c572196669afda9b183f32c645
VL - 9
ID - 5811
ER -
TY - JOUR
AB - Iron oxide (Fe3O4) nanoparticles (IONPs) have received much attention for
their utility in biomedical applications such as magnetic resonance imaging, drug
delivery and hyperthermia. Recent studies reported that IONPs induced cytotoxicity
in mammalian cells. However, little is known about the genotoxicity of IONPs
following exposure to human cells. In this study, we investigated the cytotoxicity,
oxidative stress and genotoxicity of IONPs in two human cell lines; skin epithelial
A431 and lung epithelial A549. Prepared IONPs were polygonal in shape with a smooth
surface and had an average diameter of 25 nm. IONPs (25-100 mu g/ml) induced dose-
dependent cytotoxicity in both types of cells, which was demonstrated by cell
viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide) and
lactate dehydrogenase leakage assays. IONPs were also found to induce oxidative
stress in a dose-dependent manner, evident by depletion of glutathione and
induction of reactive oxygen species (ROS) and lipid peroxidation. Comet assay
revealed that level of DNA damage was higher with concentration of IONPs in both
types of cells. Quantitative real-time PCR analysis showed that following the
exposure of cells to IONPs, the expression levels of mRNA of caspase-3 and caspase-
9 genes were higher. We also observed the higher activity of caspase-3 and caspase-
9 enzymes in IONPs treated cells. Moreover, western blot analysis showed that
protein expression level of cleaved caspase-3 was up-regulated by IONPs in both
types of cells. Taken together, our data demonstrates that IONPs have potential to
induce genotoxicity in A431 and A549 cells, which is likely to be mediated through
ROS generation and oxidative stress. This study suggests that genotoxic effects of
IONPs should be further investigated at in vivo level.
AN - WOS:000325153000011
AU - Ahamed, M.
AU - Alhadlaq, H. A.
AU - Alam, J.
AU - Khan, M.
AU - Ali, D.
AU - Alarafi, S.
DA - NOV
DO - 10.2174/1381612811319370011
IS - 37
PY - 2013
SN - 1381-6128
1873-4286
SP - 6681-6690
ST - Iron Oxide Nanoparticle-induced Oxidative Stress and Genotoxicity in Human
Skin Epithelial and Lung Epithelial Cell Lines
T2 - CURRENT PHARMACEUTICAL DESIGN
TI - Iron Oxide Nanoparticle-induced Oxidative Stress and Genotoxicity in Human
Skin Epithelial and Lung Epithelial Cell Lines
VL - 19
ID - 6479
ER -
TY - JOUR
AB - This study pertains to the new approach for the development of hybrid peptide
LL-37Tα1 and its biomedical applications. A linear cationic hybrid peptide, LL-
37Tα1 was derived from two parental peptides (LL-37 and Tα1) recognized as potent
anti-endotoxin without any hemolytic or cytotoxic activity. We successfully cloned
the gene of hybrid peptide LL-37Tα1 in PpICZαA vector and expressed in the Pichia
pastoris. The recombinant peptide was purified by Ni-affinity column and reverse-
phase high performance liquid chromatography (RP-HPLC) with an estimated molecular
mass of 3.9 kDa as determined by SDS-PAGE and mass spectrometry. We analyzed the
LPS neutralization by limulus amebocyte lysate (LAL) activity and the results
indicate that the hybrid peptide LL-37Tα1 directly binds endotoxin and
significantly (p < 0.05) neutralizes the effect of LPS in a dose-dependent manner.
Lactate dehydrogenase (LDH) assay revealed that LL-37Tα1 successfully reduces the
LPS-induced cytotoxicity in mouse RAW264.7 macrophages. Moreover, it significantly
(p < 0.05) decreased the levels of nitric oxide, proinflammatory cytokines
including TNF-α, IL-6, IL-1β, and diminished the number of apoptotic cells in LPS-
stimulated mouse RAW264.7 macrophages. Our results suggest that the P. pastoris
expression system is cost-effective for commercial production of the
immunomodulatory and anti-inflammatory hybrid peptide (IAHP) LL-37Tα1 and the
peptide may serve as effective anti-endotoxin/anti-inflammatory agent with minimal
cytotoxicity. Copyright © 2019 Ahmad, Hanif, Xubiao, Lulu, Shahid, Dayong and
Rijun. This is an open-access article distributed under the terms of the Creative
Commons Attribution License (CC BY). The use, distribution or reproduction in other
forums is permitted, provided the original author(s) and the copyright owner(s) are
credited and that the original publication in this journal is cited, in accordance
with accepted academic practice. No use, distribution or reproduction is permitted
which does not comply with these terms.
AU - Ahmad, B.
AU - Hanif, Q.
AU - Xubiao, W.
AU - Lulu, Z.
AU - Shahid, M.
AU - Dayong, S.
AU - Rijun, Z.
C7 - 1365
DB - Scopus
DO - 10.3389/fimmu.2019.01365
IS - JUN
KW - Apoptosis
Hybrid peptides
Immunomodulatory
LPS neutralization
Yeast expression
Animals
Anti-Inflammatory Agents
Antimicrobial Cationic Peptides
Chromatography, High Pressure Liquid
Cloning, Molecular
Cytokines
Fish Proteins
Immunologic Factors
Inflammation Mediators
L-Lactate Dehydrogenase
Lipopolysaccharides
Mice
Nitric Oxide
Pichia
RAW 264.7 Cells
antiinflammatory agent
cathelicidin antimicrobial peptide LL 37
cathelicidin antimicrobial peptide ll 37 t alpha 1
immunomodulating agent
interleukin 1beta
interleukin 6
lipopolysaccharide
restriction endonuclease
unclassified drug
antimicrobial cationic peptide
autacoid
CAP18 lipopolysaccharide-binding protein
cytokine
fish protein
immunologic factor
lactate dehydrogenase
nitric oxide
affinity chromatography
animal cell
apoptosis
Article
controlled study
cytotoxicity
down regulation
drug purification
electrospray mass spectrometry
hemolysis
immunomodulation
Komagataella pastoris
mouse
nonhuman
polyacrylamide gel electrophoresis
polymerase chain reaction
protein expression
RAW 264.7 cell line
reversed phase high performance liquid chromatography
silver staining
animal
genetics
high performance liquid chromatography
metabolism
molecular cloning
M3 - Article
PY - 2019
ST - Expression and purification of hybrid ll-37tα1 peptide in pichia pastoris and
evaluation of its immunomodulatory and anti-inflammatory activities by LPS
neutralization
T2 - Frontiers in Immunology
TI - Expression and purification of hybrid ll-37tα1 peptide in pichia pastoris and
evaluation of its immunomodulatory and anti-inflammatory activities by LPS
neutralization
85068956202&doi=10.3389%2ffimmu.2019.01365&partnerID=40&md5=b1298aa6cdd3fe8ae78c5a7
9f2cb5965
VL - 10
ID - 5448
ER -
TY - JOUR
AB - Nature is a rich source of natural drug-like compounds with minimal side
effects. Phytochemicals better known as "Natural Products" are found abundantly in
a number of plants. Since time immemorial, spices have been widely used in Indian
cuisine as flavoring and coloring agents. Most of these spices and condiments are
derived from various biodiversity hotspots in India (which contribute 75% of global
spice production) and form the crux of India's multidiverse and multicultural
cuisine. Apart from their aroma, flavor and taste, these spices and condiments are
known to possess several medicinal properties also. Most of these spices are
mentioned in the Ayurveda, the indigenous system of medicine. The antimicrobial,
antioxidant, antiproliferative, antihypertensive and antidiabetic properties of
several of these natural products are well documented in Ayurveda. These
phytoconstituemts are known to act as functional immunoboosters, immunomodulators
as well as anti-inflammatory agents. As anticancer agents, their mechanistic action
involves cancer cell death via induction of apoptosis, necrosis and autophagy. The
present review provides a comprehensive and collective update on the potential of
66 commonly used spices as well as their bioactive constituents as anticancer
agents. The review also provides an in-depth update of all major in vitro, in vivo,
clinical and pharmacological studies done on these spices with special emphasis on
the potential of these spices and their bioactive constituents as potential
functional foods for prevention, treatment and management of cancer.
AN - WOS:000529804200001
AU - Ahmad, R.
AU - Khan, M. A.
AU - Srivastava, A. N.
AU - Gupta, A.
AU - Srivastava, A.
AU - Jafri, T. R.
AU - Siddiqui, Z.
AU - Chaubey, S.
AU - Khan, T.
AU - Srivastava, A. K.
DO - 10.2174/1871520619666191015103712
IS - 2
PY - 2020
SN - 1871-5206
1875-5992
SP - 122-236
ST - Anticancer Potential of Dietary Natural Products: A Comprehensive Review
T2 - ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
TI - Anticancer Potential of Dietary Natural Products: A Comprehensive Review
VL - 20
ID - 6528
ER -
TY - JOUR
AB - Alcea rosea (AR) seed extracts blocked colon cancer cell proliferation,
promoted apoptosis, inhibited EZH2 and Notch and Wnt/beta-catenin signaling thereby
impeding the growth of tumor xenografts reiterating the notion that plant-derived
substances offer an effective preventative/therapeutic strategy to target
CRC.Phytochemicals modulate key cellular signaling pathways and have proven
anticancer effects. Alcea rosea (AR; Hollyhock) is an ornamental plant with known
anti-inflammatory properties. This study explored its role as an anticancer agent.
The AR seed extract (AR extract) inhibited proliferation and colony formation in a
dose- and time-dependent manner and promoted apoptosis as was evidenced by cleavage
of PARP and increased expression of Bax accompanying reduced levels of BCL-xl
protein in HCT116 and SW480 cells, respectively. In addition, AR extract-arrested
cells at Go/G1 phase of cell cycle and exhibited decreases in Cyclin D1. AR
extract-treated cells exhibited reduced number and size of colonospheres in a dose-
dependent manner concomitant with decreases in cancer stem cell (CSC) markers
ALDH1A1 and Dclk1. Relative levels of beta-catenin, Notch-ICD, Hes1 and EZH2 were
also attenuated by AR extract. TOP-flash reporter activity, a measure of Wnt
signaling, decreased significantly in response to treatment while overexpression of
wild type but not mutant EZH2, reversed the inhibitory effects. Moreover, WIF1 (a
Wnt antagonist) promoter activity increased dramatically following treatment with
AR extract which phenocopied increases in WIF1 reporter activity following EZH2
knockdown. In vivo, AR extract attenuated tumor growth due probably to reduced
levels of EZH2, beta-catenin, CyclinD1 and Ki-67 along with reduced levels of CSC
markers. Since partial purification via HPLC yielded a prominent peak, efforts are
underway to identify the active ingredient(s). Taken together, the results clearly
suggest that AR extract/active component(s) can be an effective
preventative/therapeutic agent to target colon cancer.
AN - WOS:000374245300005
AU - Ahmed, I.
AU - Roy, B. C.
AU - Subramaniam, D.
AU - Ganie, S. A.
AU - Kwatra, D.
AU - Dixon, D.
AU - Anant, S.
AU - Zargar, M. A.
AU - Umar, S.
DA - APR
DO - 10.1093/carcin/bgw009
IS - 4
PY - 2016
SN - 0143-3334
1460-2180
SP - 385-396
ST - An ornamental plant targets epigenetic signaling to block cancer stem cell-
driven colon carcinogenesis
T2 - CARCINOGENESIS
TI - An ornamental plant targets epigenetic signaling to block cancer stem cell-
driven colon carcinogenesis
VL - 37
ID - 6530
ER -
TY - JOUR
AB - In this present study, the endophytic bacteria were isolated from the
drought-tolerant ornamental plant Pennisetum setaceum. The biomass of endophytic
bacteria was utilized for the biogenic synthesis of silver nanoparticles (AgNPs).
The synthesis of AgNPs was confirmed by UV-Visible and FTIR spectroscopy followed
by SEM analysis. The antibacterial studies were performed through MIC, MBC, and
biofilm assays. Efficacy of AgNPs against the human breast cancer (MCF-7) cells was
also tested, and the IC50 was determined by MTT assay. In our study, we have
observed that the synthesized AgNPs exhibited a dose-dependent cytotoxicity (1-100
mu g/mL) against MCF-7 cells and morphological alterations of the cells were also
visualized and the IC50 was observed at 50 mu g/mL. The treatment of synthesized
AgNPs altered the expression of apoptotic proteins including Bax, Bcl-2, and
inflammatory marker COX-2 in MCF-7 cells. To the best of our knowledge, this is the
first report that demonstrates the AgNPs from endophytic bacteria isolated from the
plant Pennisetum setaceum can induce apoptosis in human breast cancer MCF-7 cells.
Our results suggest that AgNPs used in this study can be utilized to control human
pathogens and can also be utilized to induce apoptosis in breast cancer cells.
AN - WOS:000485053500041
AU - Ahmed, M. S.
AU - Soundhararajan, R.
AU - Akther, T.
AU - Kashif, M.
AU - Khan, J.
AU - Waseem, M.
AU - Srinivasan, H.
DA - SEP
DO - 10.1007/s11356-019-05869-6
IS - 26
PY - 2019
SN - 0944-1344
1614-7499
SP - 26939-26946
ST - Biogenic AgNPs synthesized via endophytic bacteria and its biological
applications
T2 - ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
TI - Biogenic AgNPs synthesized via endophytic bacteria and its biological
applications
VL - 26
ID - 6122
ER -
TY - JOUR
AB - Silver nanoparticles (SNP) are used in many pharmaceutical, cosmetic, and
industrial products already available in the market. Although they are considered
relatively safe, many toxic and pathological alterations in different organs
including immune organs were reported after SNP administration. In this study, 10-
week-old male mice (n = 20) were divided into two groups. Ten mice received greenly
synthesized gelatin-coated silver nanoparticles in a dose of 10 mg/kg body weight
for five consecutive days while the other 10 received 0.5 ml of distilled water
daily for 5 days and kept as control. At the sixth day, all mice were sacrificed;
blood and tissue samples were collected and prepared for pathological analysis.
Liver and kidney lesions were in the form of degenerative and inflammatory changes.
Interestingly, the immune organs were drastically affected by SNP treatment. Severe
hyperplasia of the Peyer's patches was noticed in the intestines of intoxicated
animals both in gross and microscopic examination. Spleen was enlarged and showed
large number of megakaryocytes. The particles were encountered in membrane-bound
phagosomes inside macrophages in different organs like lungs and spleen. Blood
picture complied to morphological findings with an increase in monocytes and
eosinophils accompanied by drop in the platelets count in the intoxicated animals.
AN - WOS:000389178600006
AU - Ahmed, O. B.
AU - Mahmoud, U. T.
AU - Elganady, S.
AU - Nafady, A. M.
AU - Afifi, S. M. H.
DA - NOV-DEC
DO - 10.1080/01913123.2016.1239666
IS - 6
PY - 2016
SN - 0191-3123
1521-0758
SP - 342-350
ST - Immunomodulatory effect of gelatin-coated silver nanoparticles in mice:
Ultrastructural evaluation
TI - Immunomodulatory effect of gelatin-coated silver nanoparticles in mice:
Ultrastructural evaluation
VL - 40
ID - 5948
ER -
TY - JOUR
AB - The demand for nanoparticles is increasing day by day due to their wide range
of applications in various areas including pharmaceutical industry. Nanoparticles
are formally synthesized by chemical methods in which the toxic and flammable
chemicals are used. Synthesis of nanoparticles from various biological systems has
been reported, but among all, biosynthesis of nanoparticles from plants is
considered as the most suitable method. The current study confirms the potential of
aqueous extract of Melissa officinalis grown under in vitro condition for the green
synthesis of silver nanoparticles (AgNPs). Also, we revealed the cytotoxicity,
antioxidant, and anti-acute myeloid leukemia effects of AgNPs compared to
mitoxantrone in a leukemic mouse model. The synthesized AgNPs were characterized
using several techniques including UV–Vis., FT-IR, TEM, FE-SEM, and EDS. In vivo
experiment, induction of acute myeloid leukemia was done by DMBA in 75 mice. The
obtained results were fed into SPSS-22 software and analyzed by one-way ANOVA. By
quantitative real-time PCR, S1PR1 and S1PR5 mRNA expression in lymphocytes were
significantly (p ≤ 0.01) increased by treating the leukemic mice with the AgNPs and
mitoxantrone. Also, AgNPs similar to mitoxantrone, significantly (p ≤ 0.01)
enhanced the platelet, lymphocyte, and RBC parameters and the anti-inflammatory
cytokines (IL4, IL5, IL10, IL13, and IFNα) and reduced the total WBC, blast,
monocyte, neutrophil, eosinophil, and basophil counts and the pro-inflammatory
cytokines (IL1, IL6, IL12, IL18, IFNY, and TNFα) as compared to the untreated mice.
In vitro experiment, AgNPs similar to mitoxantrone had low cell viability dose-
dependently against murine C1498, human HL-60/vcr, and 32D-FLT3-ITD cell lines
without any cytotoxicity on HUVEC cell line. Furthermore, the DPPH assay showed
similar antioxidant potentials for AgNPs and mitoxantrone. Above results approve
the excellent anti-acute myeloid leukemia, cytotoxicity, and antioxidant properties
of AgNPs compared to mitoxantrone. © 2019 John Wiley & Sons, Ltd.
AU - Ahmeda, A.
AU - Zangeneh, A.
AU - Zangeneh, M. M.
C7 - e5378
DB - Scopus
DO - 10.1002/aoc.5378
IS - 2
KW - acute myeloid leukemia
chemotherapeutic drug
Melissa officinalis
mitoxantrone
Antioxidants
Biochemistry
Cell culture
Cytotoxicity
Diseases
Drug delivery
Indicators (chemical)
Lymphocytes
Mammals
Metal nanoparticles
Plant extracts
Polymerase chain reaction
Synthesis (chemical)
Acute myeloid leukemia
Chemical characterization
Chemotherapeutic drugs
Mitoxantrone
Pro-inflammatory cytokines
Quantitative real time PCR
M3 - Article
PY - 2020
ST - Preparation, formulation, and chemical characterization of silver
nanoparticles using Melissa officinalis leaf aqueous extract for the treatment of
acute myeloid leukemia in vitro and in vivo conditions
T2 - Applied Organometallic Chemistry
TI - Preparation, formulation, and chemical characterization of silver
nanoparticles using Melissa officinalis leaf aqueous extract for the treatment of
acute myeloid leukemia in vitro and in vivo conditions
85076373188&doi=10.1002%2faoc.5378&partnerID=40&md5=dd2d7a210849b822bb8ca4dec2e72f2
6
VL - 34
ID - 5344
ER -
TY - JOUR
AB - Traditional synthetic techniques for silver nanoparticles synthesis involve
toxic chemicals that are harmful to humans as well as the environment. The green
chemistry method for nanoparticle synthesis is rapid, eco-friendly, and less toxic
as compared to the traditional methods. In the present research, we synthesized
silver nanoparticles employing a green chemistry approach from Parthenium
hysterophorus leaf extract. The optimized parthenium silver nanoparticles (PrSNPs)
had a mean particle size of 187.87 ± 4.89 nm with a narrow size distribution of
0.226 ± 0.009 and surface charge -34 ± 3.12mV, respectively. The physicochemical
characterization of optimized SNPs was done by Fourier transform infrared
spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning
calorimetry (DSC). Moreover, the transmission electron microscopy (TEM) analysis
indicates the spherical shape of NPs with an average diameter of 20-25 nm. PrSNPs
were investigated for in vitro antibacterial, antifungal, anti-inflammatory, and
antioxidant properties, and showed excellent profiles. The cytotoxic activity was
analyzed against two cancer cell lines, i.e., B16F10 and HepG2 for 24 h and 48 h.
PrSNPs proved to be an excellent anticancer agent. These PrSNPs were also employed
for the treatment of wastewater by monitoring the E. coli count, and it turned out
to be reduced by 58%; hence these NPs could be used for disinfecting water. Hence,
we can propose that PrSNPs could be a suitable candidate as an antimicrobial,
antioxidant, anti-inflammatory, and antitumor agent for the treatment of several
ailments. © 2020 by the authors.
AU - Ahsan, A.
AU - Farooq, M. A.
AU - Bajwa, A. A.
AU - Parveen, A.
C7 - 3324
DB - Scopus
DO - 10.3390/molecules25153324
IS - 15
KW - Anti-inflammatory
Antimicrobial
Antioxidant
Cytotoxicity
Green chemistry
Parthenium hysterophorus
Antineoplastic Agents
Antioxidants
Cell Line, Tumor
Cell Survival
Escherichia coli
Green Chemistry Technology
Humans
Metal Nanoparticles
Plant Extracts
Plant Leaves
Silver
Treatment Outcome
antiinfective agent
antineoplastic agent
antioxidant
metal nanoparticle
Parthenium hysterophorus extract
plant extract
silver
cell survival
chemistry
drug effect
green chemistry
human
plant leaf
treatment outcome
tumor cell line
M3 - Article
PY - 2020
ST - Green synthesis of silver nanoparticles using parthenium hysterophorus:
Optimization, characterization and in vitro therapeutic evaluation
T2 - Molecules
TI - Green synthesis of silver nanoparticles using parthenium hysterophorus:
Optimization, characterization and in vitro therapeutic evaluation
85088678393&doi=10.3390%2fmolecules25153324&partnerID=40&md5=a4fafc4f48e2eb4e1ba67b
1561b4c879
VL - 25
ID - 5336
ER -
TY - JOUR
AB - Recent advances in science and technology and greatly modified the way we
stumble on, deal with and prevent special diseases in all components of human
lifestyles. Rheumatoid arthritis (RA) is the most not unusual complex
multifactorial joint related autoimmune, chronic, severe systemic inflammatory
ailment with unknown etiology completed with increased cardiovascular risks. It is
regularly associated with critical synovial joint inflammation, autoantibody
production, cartilage/bone tissue destruction, cardiovascular, pulmonary, skeletal
disorders and massive inflammatory infiltration which might in the end motive
extreme disability, huge complications, premature mortality and decreased life
quality. Pro-inflammatory cytokines like IL-1, IL-6, IL-8 and IL-10 were dependable
for the induction of inflammation in RA patients. It has a global occurrence of
around 1% with the incidence among women being 2-3 times extra in men. Preclinical
RA, genetic variables, and environmental factors have all been linked to the
disease's etiology. Because there is no known cure for RA, the primary goal of
treatment is to achieve the shortest possible illness duration and, if possible,
rehabilitation. Current clinical remedies of RA display numerous drawbacks which
include excessive doses, common administration, speedy metabolism, bad absorption,
low responsiveness, higher cost and serious side consequences. These obstacles have
inspired extremely good growth of the studies and to enhance those obstacles,
nanoparticles that are able to encapsulating and protecting tablets from
degradation earlier than they reach the target site in vivo, might also function
drug delivery structures. Bioavailability and therapeutic bioactivity can be
improved, and limited emphasis on damaged joints can be allowed. The current study
provides a platform for different lipid nanoparticle methods for RA therapy, using
the newly developing field of lipid nanoparticles to improve a targeted theranostic
device for RA treatment. This review aims to present the most recent major
application of lipid nanoparticles as a biocompatible and biodegradable transport
device for improving RA concentration on over free drugs by presenting tissue-
specific concentrated on of ligand-controlled drug release by modulating
nanoparticle composition. Additionally, we also discuss the pivotal demanding
situations to be addressed, as well as destiny views. Therefore, it is feasible to
claim that nanoparticles will, within the near future, play a critical role in
advanced treatment and affected person-particular cures for human diseases which
include RA. © 2021 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
AU - Ahuja, N. K.
AU - Rajawat, J. S.
DB - Scopus
DO - 10.22159/ijap.2021v13i6.42912
IS - 6
KW - Drug delivery
Inflammation
Lipid nanoparticle
Rheumatoid arthritis
aceclofenac
actarit
autoantibody
C reactive protein
CD40 ligand
celecoxib
curcumin
cyclooxygenase 2
cyclosporine
cystatin C
cytokine
dexamethasone
dexamethasone sodium phosphate
doxorubicin
gelatinase B
glycerol stearate
glycosaminoglycan
hydrogel
interleukin 1
interleukin 10
interleukin 6
interleukin 8
lipid nanoparticle
liposome
macrogol
methotrexate
monocyte chemotactic protein 1
nanocarrier
nanochain
nanocomposite
nanoparticle
piperine
piroxicam
polyglactin
polymer
silver nanoparticle
solid lipid nanoparticle
tacrolimus
antimicrobial activity
autoimmune thyroiditis
bioavailability
biocompatibility
controlled drug release
drug bioavailability
drug delivery system
drug release
encapsulation
environmental factor
human
inflammation
major histocompatibility complex
nanotechnology
nonhuman
Review
rheumatoid arthritis
zeta potential
M3 - Review
PY - 2021
SP - 31-40
ST - Novel nano therapeutic materials for the effective treatment of rheumatoid
arthritis-recent insights
T2 - International Journal of Applied Pharmaceutics
TI - Novel nano therapeutic materials for the effective treatment of rheumatoid
arthritis-recent insights
85119059996&doi=10.22159%2fijap.2021v13i6.42912&partnerID=40&md5=d88756a71c72ac50ff
21b92dedaf53c2
VL - 13
ID - 5188
ER -
TY - JOUR
AB - By-products from the sugar industry (e.g., blackstrap molasses) can be a
source of bioactive compounds (e.g., phenolics) known to have antimicrobial,
antioxidant, anticancer, and anti-inflammatory properties. These bioactive
molecules can be used in the green synthesis of metal nanoparticles. Metal
nanoparticles have gathered attention because of their novel physico-chemical
properties and potential biological applications (e.g., biocides, fungicides,
pesticides, targeted drug and gene delivery, biosensing, medical implants, and
plant biostimulants). Gold, silver, iron, and copper nanoparticles are of
particular interest as they can be easy to operate and are cost effective, and
biocompatible, and their biological activities can be enhanced by surface
modifications. In this study, the reducing potential of the phenolic compounds in
molasses was investigated for the synthesis of silver nanoparticles without the
external addition of reducing agents. The reddish color formation and peak
appearance at 420 nm were indications of the successful synthesis of the silver
nanoparticles. The synthesized nanoparticles and reducing biomolecules were further
characterized by microscopy (SEM, TEM, EDS) and spectroscopy (FTIR) techniques
indicating nanoparticles of spherical shape and with particle sizes ranging from 15
nm-45 nm. Their antimicrobial activity was evaluated against several Gram-positive
and Gram-negative bacteria. The synthesized nanoparticles showed a biocidal effect,
further confirmed by microscopy techniques. It appears that the nanoparticles are
interacting with the cell surface of bacteria, penetrating the cell and also
causing the disruption of intracellular organelles.
AN - WOS:000983223300060
AU - Aita, G. M.
AU - Moon, Y. H.
DA - APR
DO - 10.36961/si29706
IS - 4
PY - 2023
SN - 0344-8657
SP - 206-213
ST - Blackstrap molasses as a source of bioactive compounds for the green
synthesis of nanoparticles
T2 - SUGAR INDUSTRY-ZUCKERINDUSTRIE
TI - Blackstrap molasses as a source of bioactive compounds for the green
synthesis of nanoparticles
VL - 148
ID - 6179
ER -
TY - JOUR
AB - This review examines the interaction of nanomaterials (NMs) with cells from
the perspective of major cellular differentiations. The structure and composition
of cells reflect their role and function in a particular organ or environment. The
normal differentiated-state and diseased cells may respond to NMs very differently.
This review progresses with due care on nanotoxicology while emphasizing the
potential of NMs in treating stress associated disorders, including cancer and
degeneration. The striking potential of NMs in inducing ROS, scavenging ROS,
depleting cellular antioxidants, replenishing antioxidants, mimicking antioxidant
enzyme activity, and modulating the immune system all show their considerable
potential in treating cancer and other aging-associated disorders. It is now clear
that NMs become more active and versatile when they come into contact with
biological machinery, surprisingly in some cases, in a manner dependent on cell
type. The mechanisms leading to the contrasting bioresponse of NMs ranging from
toxicity to anticancer and from cell survival to carcinogenicity followed by their
immuno-modulating potential show NMs to be a highly promising agent in biomedical
therapy. This first-of-its-kind article seeks the challenges to be addressed that
could provide a solid rationale in translating the promises of nanomedicine. A
thorough understanding of normal and cancer biology could help to minimize the gap
between basic and translational research in nanotechnology-based therapy.
AN - WOS:000451491300031
AU - Akhtar, M. J.
AU - Ahamed, M.
AU - Alhadlaq, H. A.
DA - DEC
DO - 10.1016/j.cca.2018.10.004
PY - 2018
SN - 0009-8981
1873-3492
SP - 186-196
ST - Challenges facing nanotoxicology and nanomedicine due to cellular diversity
T2 - CLINICA CHIMICA ACTA
TI - Challenges facing nanotoxicology and nanomedicine due to cellular diversity
VL - 487
ID - 6707
ER -
TY - JOUR
AB - SUMMARY: Adriamycin (ADR) is an anthracycline antibiotic used for treatment
of many types of cancer. However, its applications may damage to healthy tissues.
Chloroquine (CLQ) is an anti-inflammatory agent used in treatment of many
inflammation associated diseases such as malaria and rheumatoid arthritis.
Moreover, it is used in the treatment of pneumonia caused by COVID-19. The aim of
this study is to determine possible therapeutic effects of Chloroquine on
Adriamycin-induced testicular toxicity in rats. We investigated the effect of CLQ
on testicular injury caused by ADR. Rats were divided into four groups: Control,
ADR, CLQ, ADR+CLQ. After administrations, animals were sacrificed, and testis
tissues were extracted from the animals for the further examinations.
Histopathological changes in testis tissues were evaluated and TNF-α and IL-6
immunostaining were performed to determine the expression levels of these
cytokines. TUNEL method were used for evaluation of apoptotic index. Moreover,
serum testosterone levels were measured by ELISA assay. We observed that ADR group
showed histopathological deterioration when compared to the Control group and CLQ
treatment ameliorated this damage induced by Adriamycin.An increase in TNF-α and
IL-6 immunoreactivities and in the number of apoptotic cells and a decrease in
serum testosterone levels were determined in the ADR group compared to the Control
and CLQ group. Furthermore, our examinations showed an improvement in testicular
tissue in ADR+CLQ group in terms of these parameters when compared to the ADR
group. We suggest that CLQ can be used as a protective agent to reduce the toxic
effects of Adriamycin as a result of its anti-inflammatory and anti-apoptotic
properties.
RESUMEN: La adriamicina (ADR) es un antibiótico de antraciclina que se usa para el
tratamiento de muchos tipos de cáncer. Sin embargo, sus aplicaciones pueden dañar
los tejidos sanos. La cloroquina (CLQ) es un agente antiinflamatorio que se utiliza
en el tratamiento de enfermedades asociadas a la inflamación, tal como la malaria y
la artritis reumatoide. También se utiliza en el tratamiento de la neumonía causada
por COVID-19. El objetivo de este estudio fue determinar los posibles efectos
terapéuticos de la cloroquina sobre la toxicidad testicular inducida por
adriamicina en ratas. Investigamos el efecto de CLQ sobre la lesión testicular
causada por ADR. Las ratas se dividieron en cuatro grupos: Control, ADR, CLQ, ADR +
CLQ. Después de las administraciones, se sacrificaron los animales y se extrajeron
los testículos de los animales para los exámenes adicionales. Se evaluaron los
cambios histopatológicos en los tejidos testiculares y se realizó la inmunotinción
de TNF-α e IL-6 para determinar los niveles de expresión de estas citocinas. Se
utilizó el método TUNEL para la evaluación del índice apoptótico. Además, los
niveles de testosterona en suero se midieron mediante un ensayo ELISA. El grupo ADR
mostró un deterioro histopatológico en comparación con el grupo Control y
observamos que el tratamiento con CLQ mejoró el daño inducido por Adriamicina. Un
aumento en las inmunorreactividades de TNF-α e IL-6 y en el número de células
apoptóticas además de una disminución en los niveles séricos de testosterona se
determinaron en el grupo de ADR en comparación con el grupo de control y CLQ.
Además, nuestros exámenes mostraron una mejora en el tejido testicular en el grupo
ADR + CLQ en términos de estos parámetros en comparación con el grupo ADR.
Sugerimos que CLQ se puede utilizar como agente protector para reducir los efectos
tóxicos de la Adriamicina, gracias a sus propiedades antiinflamatorias y
antiapoptóticas.
AD - Akin, Ali Tugrul
Erciyes University. Science Faculty. Department of Biology. Kayseri. TR
Kaymak, Emin
Yozgat Bozok University. Faculty of Medicine. Histology-Embryology Department.
Yozgat. TR
Öztürk, Emel
Harran University. Faculty of Medicine. Histology-Embryology Department. Sanliurfa.
TR
Karabulut, Derya
Erciyes University. Faculty of Medicine. Histology-Embryology Department. Kayseri.
TR
Toluk, Ayse
Erciyes University. Science Faculty. Department of Biology. Kayseri. TR
AU - Akin, Ali Tugrul
AU - Kaymak, Emin
AU - Öztürk, Emel
AU - Karabulut, Derya
AU - Toluk, Ayse
C1 - 20220812
DA - 2021/08
DB - LILACS
DO - 10.4067/S0717-95022021000401123
IS - 4
KW - Chemotherapy
Daño de testículo
IL-6
Quimioterapia
TNF-&#945
TNF-a
TUNEL
Testis damage
LA - en
PY - 2021
SN - 0717-9367
SP - 1123-1131
ST - Chloroquine ameliorates adriamycin-induced testicular damage by suppressing
the inflammation and apoptosis in rats: a histological, immunohistochemical and
biochemical study
T2 - Int. j. morphol
TI - Chloroquine ameliorates adriamycin-induced testicular damage by suppressing
the inflammation and apoptosis in rats: a histological, immunohistochemical and
biochemical study
TT - La cloroquina mejora el daño testicular inducido por la adriamicina al
suprimir la inflamación y la apoptosis en ratas: un estudio histológico,
inmunohistoquímico y bioquímico
UR - http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-
95022021000401123&lng=en&nrm=iso&tlng=en
VL - 39
ID - 4918
ER -
TY - JOUR
AB - Zinc oxide nanoparticles (ZnO NPs) are one of the prominent metal oxide
nanoparticles with significant applications in many industries and research
institutes. Various methods of synthesis have been adopted in the production of ZnO
NPs so as to meet its high demand. The environmental implications and economic
challenges attached to most of the means of ZnO NPs synthesis have resulted in the
quest for other alternatives with environmental and economic benefits.
Interestingly, the biological method of synthesis using plant sources have been
found appropriate for the production of ZnO NPs dues to its numerous health,
environmental, economic, and medicinal benefits. The distinctive features of ZnO
NPs synthesized using plant extracts enhanced its application in agriculture for
the production of fertilizers, pesticides, and fumigants. In the field of medicine
and pharmacy, phytosynthesized ZnO NPs have gained remarkable usage in the
production of disinfectant, antifungal, anticancer, antioxidant, anti-inflammatory
and antidiabetics agents. Despite the enlisted benefits of biosynthesized ZnO NPs,
the difficulties associated with the elucidation of formation mechanism and
reactions still remain unraveled. This review described the summary of the recent
advances in the synthesis, mechanism routes, characterization techniques, and
applications of biosynthesized ZnO NPS in agriculture, medicine, and textile
industries. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
AU - Akintelu, S. A.
AU - Folorunso, A. S.
DB - Scopus
DO - 10.1007/s12668-020-00774-6
IS - 4
KW - Green synthesis
Nanoparticles
Plant extracts and biomedical applications
Zinc oxide
Agricultural robots
Agriculture
Antifungal agents
Fumigation
Green Synthesis
Medicine
Metal nanoparticles
Metals
Oxide minerals
Pesticides
Plant extracts
Textile industry
ZnO nanoparticles
alkaloid
antioxidant
disinfectant agent
flower extract
metal oxide
nanomaterial
nanoparticle
pesticide
plant extract
saponin
silver nanoparticle
terpenoid
Anti-inflammatories
Characterization techniques
Environmental implications
Formation mechanism
Metal oxide nanoparticles
Methods of synthesis
antifungal activity
biocompatibility
biosynthesis
Culex tritaeniorhynchus
cytotoxicity
environmental health
Escherichia coli
Euphorbia
human
Laurus nobilis
medical research
medicinal plant
MG-63 cell line
nonhuman
photocatalysis
phytochemistry
plant growth
quince
Review
stomach cancer
textile industry
X ray diffraction
II-VI semiconductors
M3 - Review
PY - 2020
SP - 848-863
ST - A Review on Green Synthesis of Zinc Oxide Nanoparticles Using Plant Extracts
and Its Biomedical Applications
T2 - BioNanoScience
TI - A Review on Green Synthesis of Zinc Oxide Nanoparticles Using Plant Extracts
and Its Biomedical Applications
85088506551&doi=10.1007%2fs12668-020-00774-
6&partnerID=40&md5=05f9760f2039450ac15e75e6fcaaedf2
VL - 10
ID - 5271
ER -
TY - JOUR
AB - Background: Ulcerative colitis (UC) is a debilitating chronic inflammatory
bowel disease that is prevalent in Egypt. Current treatment of UC is expensive and
has serious side effects. Aim of the Work: To evaluate the effect of ginger loaded
nanoparticles (GDNP) compared to ginger extract (GE) as an alternative treatment of
acute UC (AUC) in DSS/ ethanol rat model. Material and Methods: Twenty rats were
used. AUC was induced by administration of 2% synthetic dextran sulfate sodium
(DSS). Rats were divided into 4 groups: control (GP I), AUC (GP II), AUC received
2.5 gm GE (GP III) and AUC received 2.5 gm superparamagnetic@ silver nanoparticles
GDNP (GP VI). Colonic and rectal tissue were assessed histologically using
Hematoxylin and eosin (H&E), Periodic acid- Schiff (PAS), Toluidine blue, and
electron microscopy (EM). Epithelial proliferation and apoptosis were assessed by
anti-KI-67 antibody anti BCL2 antibody. Results: GP III and GP IV showed
improvement with GDNP and GE compared to control. Gross damage scores were as
follows: GPI 0±0, GPII 2.6±1.1, GPIII 1.4±1.1, and GPIV 0±0 with significant
variation (P = 0.000). Cumulative histopathological score of GPI, II, III, and IV
were 0±0, 9.2±3.3, 3±1.6, and 1±1 respectively with significant correlation (P=
0.000). GPIII and GPIV displayed strong positive nuclear and cytoplasmic staining
for KI-67 (GPIII Mean=50±15/ HPF). (GPIV Mean=66±8/ HPF) and BCL2 (Mean=60±16 and
76±9/ HPF) with significant variation (P= 0.000). Conclusion: GDNP has more
potential in treatment of AUC compared to GE. Treatment with GDNPs improved signs,
reduced apoptosis and enhanced repair of AUC more significantly EJH copyright ©
2022. All rights served.
AU - Al-Badawi, M. H.
AU - Waly, N. E.
AU - Eid, M. M.
AU - Soliman, N. A.
DB - Scopus
DO - 10.21608/ejh.2021.68124.1448
IS - 2
KW - BCL2
ginger
ginger loaded nanoparticles
KI-67
ulcerative colitis
M3 - Article
PY - 2022
SP - 442-456
ST - Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract
as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis
T2 - Egyptian Journal of Histology
TI - Histopathological Impact of Ginger Loaded Nanoparticle Versus Ginger Extract
as A Novel Therapy of Experimentally Induced Acute Ulcerative Colitis
85144743574&doi=10.21608%2fejh.2021.68124.1448&partnerID=40&md5=9cc1f5282261130cfe7
cf2f9f1a31cb0
VL - 45
ID - 5151
ER -
TY - JOUR
AB - Both Silver (AgNPs) and gold (AuNPs) nanoparticles are increased being
utilized broadly in many industries and biomedical products to enhance their
performance. However, humans are increasingly being exposed to the two metal-NPs,
which also been shown to be highly potential toxic to mammals. Meta-NPs has been
demonstrated to have the capability to cross biological barriers cell membranes and
subsequently interact with intracellular structures. The present in vivo study
assessed the toxicological potential of AgNPs and AuNPs, in 30 mature male albino
rats, assigned to three groups, to receive intraperitoneal injection of 0.25 mg/kg
b. w of AgNPs (G1) or AuNPs (G2) or vehicle only (G3) daily for 21 days. Liver
function makers, thyroid function hormones, testosterone hormone, inflammatory
biomarkers and plasma proteins as well as histological characteristics were tested.
The results revealed increased liver enzymes indicating liver toxicity and injury.
Liver inflammation was manifested by elevated inflammatory cytokines interleukin-6
(IL-6) and tumour-necrosis factor alpha (TNF-alpha). Thyroid hormones were elevated
indicating hyperthyroidism, while testosterone hormone was diminished indicating
their potential to cause infertility in males. However, the low-dose of AgNPs and
AuNPs has no noticeable changes in histomorphologic picture of liver. In
conclusion, both metal-NPs are potentially toxic, with AgNPs exhibiting a greater
toxicity effect than AuNPs. Toxicity mechanisms include direct cellular injury
(lysis) and induction of oxidative stress. Copyright (C) 2013 - All Rights Reserved
- Pharmacophore
AN - WOS:000429049400006
AU - Al-Bishri, W. M.
DA - JAN
IS - 1
PY - 2018
SN - 2229-5402
SP - 48-55
ST - TOXICITY STUDY OF GOLD AND SILVER NANOPARTICLES ON EXPERIMENTAL ANIMALS
T2 - PHARMACOPHORE
TI - TOXICITY STUDY OF GOLD AND SILVER NANOPARTICLES ON EXPERIMENTAL ANIMALS
VL - 9
ID - 5935
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NPs) are widely used in nanomedicine, pharmaceutical
products, industry and other consumer products owing to their unique physiochemical
properties with probable potential risk to human health and the ecosystems. The aim
of this work was to investigate the in-life morphological effects, biochemical,
histological and histochemical alterations that might be induced by variable sizes
of Ag NPs in hepatic, renal and testicular tissues with the hypothesis that
variable sizes of nano-Ag could induce variable effects in the vital organs. Five
groups of adult healthy male mice (BALB/C) were exposed to 35 intraperitoneal
injections of Ag NPs (1 mg/kg bw) using five different particle sizes (10, 20, 40,
60 and 100 nm). All mice were subjected to in-life morphometric, biochemical,
histological and histochemical analysis. The findings demonstrated that Ag NPs
could induce alterations in the average body weight gain, food consumption, water
intake and organ indices. In addition, these NPs significantly altered hepatic and
renal biomarkers. Moreover, Ag NPs produced ground glass hepatocyte cytoplasm, with
mitotic activity, nuclear alterations, degeneration, glycogen depletion and
inflammatory cells infiltration in the liver. The kidneys of treated mice exhibited
proximal renal tubules degeneration, distal renal tubules regeneration, glomerular
shrinkage, Bowman's capsule thickening and interstitial inflammation. The
testicular tissues demonstrated spermatocyte sloughing and spermatid giant cell
formation. The findings together indicated that Ag NPs could interact with the
anatomical structures of the liver, kidney and testis in ways that could induce
injury. In addition, the results indicated that smaller Ag NPs posed a greater
potential risk than the larger ones, which might be associated with their
behaviour, dissolution rate, bioavailability and their probable variable
toxicokinetics.
AN - WOS:000549952200001
AU - Al-Doaiss, A. A.
AU - Jarrar, Q.
AU - Alshehri, M.
AU - Jararr, B.
C6 - JUL 2020
C7 - 0748233720937201
DA - AUG
DO - 10.1177/0748233720937201
IS - 8
PY - 2020
SN - 0748-2337
1477-0393
SP - 540-557
ST - In vivostudy of silver nanomaterials' toxicity with respect to size
T2 - TOXICOLOGY AND INDUSTRIAL HEALTH
TI - In vivostudy of silver nanomaterials' toxicity with respect to size
VL - 36
ID - 6059
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NPs) are widely used in nanomedicine, pharmaceutical
products, industry and other consumer products owing to their unique physiochemical
properties with probable potential risk to human health and the ecosystems. The aim
of this work was to investigate the in-life morphological effects, biochemical,
histological and histochemical alterations that might be induced by variable sizes
of Ag NPs in hepatic, renal and testicular tissues with the hypothesis that
variable sizes of nano-Ag could induce variable effects in the vital organs. Five
groups of adult healthy male mice (BALB/C) were exposed to 35 intraperitoneal
injections of Ag NPs (1 mg/kg bw) using five different particle sizes (10, 20, 40,
60 and 100 nm). All mice were subjected to in-life morphometric, biochemical,
histological and histochemical analysis. The findings demonstrated that Ag NPs
could induce alterations in the average body weight gain, food consumption, water
intake and organ indices. In addition, these NPs significantly altered hepatic and
renal biomarkers. Moreover, Ag NPs produced ground glass hepatocyte cytoplasm, with
mitotic activity, nuclear alterations, degeneration, glycogen depletion and
inflammatory cells infiltration in the liver. The kidneys of treated mice exhibited
proximal renal tubules degeneration, distal renal tubules regeneration, glomerular
shrinkage, Bowman’s capsule thickening and interstitial inflammation. The
testicular tissues demonstrated spermatocyte sloughing and spermatid giant cell
formation. The findings together indicated that Ag NPs could interact with the
anatomical structures of the liver, kidney and testis in ways that could induce
injury. In addition, the results indicated that smaller Ag NPs posed a greater
potential risk than the larger ones, which might be associated with their
behaviour, dissolution rate, bioavailability and their probable variable
toxicokinetics. © The Author(s) 2020.
AU - Al-Doaiss, A. A.
AU - Jarrar, Q.
AU - Alshehri, M.
AU - Jarrar, B.
DB - Scopus
DO - 10.1177/0748233720937201
IS - 8
KW - kidney
liver
nanotoxicity
testis
Animals
Kidney
Liver
Liver Function Tests
Male
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Silver
Testis
glycogen
silver nanoparticle
metal nanoparticle
silver
adult
animal experiment
animal model
animal tissue
Article
bioavailability
biochemical composition
body weight gain
Bowman capsule
cell infiltration
cell nucleus
controlled study
cytoplasm
cytotoxicity
degenerative disease
dissolution
fluid intake
food intake
glomerulopathy
histochemistry
histopathology
in vivo study
inflammatory cell
kidney distal tubule
kidney injury
kidney interstitium
kidney tissue
liver cell
liver injury
liver tissue
liver toxicity
male
mitosis
morphology
mouse
nephritis
nephrotoxicity
nonhuman
particle size
reproductive toxicity
spermatid
spermatocyte
testis injury
testis tissue
toxicokinetics
animal
Bagg albino mouse
comparative study
drug effect
liver function test
pathology
M3 - Article
PY - 2020
SP - 540-557
ST - In vivo study of silver nanomaterials’ toxicity with respect to size
T2 - Toxicology and Industrial Health
TI - In vivo study of silver nanomaterials’ toxicity with respect to size
85088147027&doi=10.1177%2f0748233720937201&partnerID=40&md5=3a14f154c06577bb6295b36
991387eae
VL - 36
ID - 5282
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NPs) are invested in various sectors and are
becoming more persistent in our ambient environment with potential risk on our
health and the ecosystems. The current study aims to investigate the histological,
histochemical and ultrastructural hepatic changes that might be induced by 10 nm
silver nanomaterials. Male mice (BALB/C) were exposed for 35 injections of daily
dose of 10 nm Ag NPs (2 mg/kg). Liver tissues were subjected to examination by
light and electron microscopy for histological, histochemical and ultrastructural
alterations. Exposure to Ag NPs induced Kupffer cells hyperplasia, sinusoidal
dilatation, apoptosis, ground glass hepatocytes appearance, nuclear changes,
inflammatory cells infiltration, hepatocytes degeneration and necrosis. In
addition, 10 nm Ag NPs induced histochemical alterations mainly glycogen depletion
with no hemosiderin precipitation. Moreover, these nanomaterials exhibited
ultrastructure alterations including mitochondrial swelling and cristolysis,
cytoplasmic vacuolation, apoptosis, multilammellar myelin figures formation and
endoplasmic destruction and reduction. The findings revealed that Ag NPs can induce
alterations in the hepatic tissues, the chemical components of the hepatocytes and
in the ultrastructure of the liver. One may also conclude that small size Ag NPs,
which are increasingly used in human products could cause various toxigenic
responses to all hepatic tissue components. © The Institution of Engineering and
Technology 2020
AU - Al-Doaiss, A.
AU - Jarrar, Q.
AU - Moshawih, S.
DB - Scopus
DO - 10.1049/iet-nbt.2020.0039
IS - 5
KW - Animals
Apoptosis
Hepatocytes
Liver
Male
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Mitochondria
Particle Size
Silver
Cell death
Cell proliferation
Health risks
Histology
Mammals
Metal nanoparticles
Microstructure
Nanostructured materials
Tissue
metal nanoparticle
silver
Ambient environment
Chemical component
Hepatic tissue
Inflammatory cells
Mitochondrial swelling
Myelin figures
Potential risks
animal
apoptosis
Bagg albino mouse
chemistry
cytology
drug effect
liver
liver cell
male
mitochondrion
mouse
particle size
pathology
M3 - Article
PY - 2020
SP - 405-411
ST - Hepatic histopathological and ultrastructural alterations induced by 10 nm
T2 - IET Nanobiotechnology
TI - Hepatic histopathological and ultrastructural alterations induced by 10 nm
85088352128&doi=10.1049%2fiet-
nbt.2020.0039&partnerID=40&md5=5a2ad42481b314d84ad7eee4371788ee
VL - 14
ID - 5319
ER -
TY - JOUR
AB - Vicia faba seeds are a rich source of polyphenolic, and flavonoid compounds
that have high antioxidant activity. The aqueous extract of V. faba seeds has been
used for the biosynthesis of a nanocomposite (NC) consisting of three different
types of nanoparticles that are deposited onto each other as C@Cu2O@Cu.
Physicochemical, optical, electrical, and morphological properties of the as-
biofabricated C@Cu2O@Cu NC were characterized using different analytical techniques
as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR),
Ultraviolet-visible spectroscopy (UV-Vis), Photoluminescence (PL), Transmission
Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Energy Dispersive X-
Ray (EDX), Raman, Zeta-potential, and water/moisture content). Results confirmed
that V. faba seeds aqueous extract mediated the fabrication of C@Cu2O@Cu NC at high
and pure crystalline nature. The nano-formulation had a face center cubic
crystallographic system at the nano-scale range using Debye-Scherrer's equation
(13.8 nm) in XRD analysis and (20.9 +/- 6.2 nm) using TEM analysis. The
crystallinity index (1.6) with a large surface area and polycrystalline nature were
being investigated. In addition, the biological activity of the synthesized NC was
analyzed. C@Cu2O@Cu NC showed high antiinflammatory activity compared with
diclofenac potassium with IC50 = 213.3 mg/mL. Moreover, the nano-formulation had a
potent antibacterial activity, particularly against Gram-negative bacteria.
Cytotoxicity of the C@Cu2O@Cu NC against MCF7, HCT116, and HepG2 cell lines showed
efficient cytotoxic impact with IC50 = 84.8, 116.1, and 120.5 mg/mL, respectively.
So, the nanocomposite C@Cu2O@Cu may provide a promising platform for the effective
treatment of different types of cancer. (C) 2021 The Author(s). Published by
Elsevier B.V.
AN - WOS:000702864200003
AU - Al-Hakkani, M. F.
AU - Hassan, S. H. A.
AU - Saddik, M. S.
AU - El-Mokhtar, M. A.
AU - Al-Shelkamy, S. A.
C6 - AUG 2021
DA - SEP-OCT
DO - 10.1016/j.jmrt.2021.07.076
PY - 2021
SN - 2238-7854
2214-0697
SP - 1998-2016
ST - Bioengineering, characterization, and biological activities of C@Cu2O@Cu
nanocomposite based-mediated the Vicia faba seeds aqueous extract
T2 - JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
TI - Bioengineering, characterization, and biological activities of C@Cu2O@Cu
nanocomposite based-mediated the Vicia faba seeds aqueous extract
VL - 14
ID - 6388
ER -
TY - JOUR
AB - Gold nanoparticles with tiny sizes and biostability are particularly
essential and are employed in a variety of biomedical applications. Using a
reducing agent and a stabilising agent to make gold nanoparticles has been reported
in a number of studies. Gold nanoparticles with a particle size of 25.31 nm were
synthesized in this study utilising Hylocereus polyrhizus (Red Pitaya) extract,
which functions as a reducing and stabilising agent. The extract of Red Pitaya is
said to be a powerful antioxidant and anti-cancer agent. Because of its substantial
blood biocompatibility and physiological stability, green production of gold
nanoparticles with H. polyrhizus fruit extract is an alternative to chemical
synthesis and useful for biological and medical applications. The formation and
size distribution of gold nanoparticles were confirmed by HPLC, UV-Vis
spectrophotometer, X-ray diffraction (XRD), Dynamic light scattering (DLS), Zeta
potential, Transmission electron microscopy (TEM), Fourier transformed infrared
spectroscopy (FTIR), Energy dispersive X-ray (EDX) and X-ray photoelectron
spectroscopy (XPS). The well-analysed NPs were used in various biological assays,
including anti-diabetic, anti-inflammatory, anti-Alzheimer, and antioxidant (DPPH),
and cytotoxic investigations. The NPs also showed a dose-dependent cytotoxic
activity against HCT-116, HepG2 and MCF-7 cell lines, with IC50 of 100 mg/mL for
HCT-116 cells, 155 mg/mL for HepG2, and for MCF-7 cells the value was 165 mg/mL
respectively. Finally, the outstanding biocompatibility of Au-NPs has led to the
conclusion that they are a promising choice for various biological applications.
(c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud
University. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/).
AN - WOS:000788024000024
AU - Al-Radadi, N. S.
C6 - MAR 2022
DA - APR
DO - 10.1016/j.sjbs.2022.01.001
IS - 4
PY - 2022
SN - 1319-562X
2213-7106
SP - 2836-2855
ST - Biogenic proficient synthesis of (Au-NPs) via aqueous extract of Red Dragon
Pulp and seed oil: Characterization, antioxidant, cytotoxic properties, anti-
diabetic anti-inflammatory, anti-Alzheimer and their anti-proliferative potential
against cancer cell lines
T2 - SAUDI JOURNAL OF BIOLOGICAL SCIENCES
TI - Biogenic proficient synthesis of (Au-NPs) via aqueous extract of Red Dragon
Pulp and seed oil: Characterization, antioxidant, cytotoxic properties, anti-
diabetic anti-inflammatory, anti-Alzheimer and their anti-proliferative potential
against cancer cell lines
VL - 29
ID - 6141
ER -
TY - JOUR
AB - The progress in the innovative nanocrystal synthesis process by using
environmentally benign and low-priced nontoxic chemicals, solvents, and renewable
sources remains a challenging task for researchers worldwide. The majority of the
existing synthesis techniques engage in the potentially dangerous, for either human
health or the environment. Current investigation has been centered on green
synthesis pro-cesses to create novel nanomaterials, which are eco-friendly as well
as safer for sustainable marketable feasibility. The current work provides the
green synthesis method for gold nanoparticle (GNPs) synthesis using Commiphora
myrrh (C.myrrh) extract. This simple method includes 6 ml of HAuCl4.3H2O treated
with 4 ml C.myrrh extract having pH 4.5 after 80 min at 25 degrees C temperature.
In this novel method, green synthesized GNPs characterized by UV-Vis, X_ray
diffraction spectroscopy (XRD), zeta potential, fourier transform infrared (FT_IR),
high_resolution transmission electron microscopy (HR_TEM), energy disper-sive X_ray
spectroscopy (EDXA), and dynamic light scattering (DLS). During the development
successful antioxidant assay, the DPPH assay was applied. The cell toxicity of
green synthesized GNPs was evaluated following an MTT assay against HCT-116 (colon
cancer) and MCF-7 (breast cancer). Besides molecular docking in the d-elemene for
inhibitor to VEGFR-2 domain revealed more negative docking score (-3.976) which is
an excellent binding affinity to the C.myrrh@GNP. The synthesized GNPs showed
antidiabetic, antibiotic, and antibacterial properties and anti_inflammatory
inhibition against inhibiting COX-1, and COX-2 enzymes. In addition, molecular
docking by Lindestrene (-3.806) and Furanoeudesma-1,3-dien (-3.912) against COX1
and COX2 respectively showed strong binding affin-ity. The molecular docking study
evidenced the anti-inflammatory and cell toxicity study.(c) 2022 The Author(s).
Published by Elsevier B.V. on behalf of King Saud University. This is an open
access article under the CC BY-NC-ND license
AN - WOS:000867484200001
AU - Al-Radadi, N. S.
DA - SEP
DO - 10.1016/j.jsps.2022.06.028
IS - 9
PY - 2022
SN - 1319-0164
2213-7475
SP - 1215-1242
ST - Single-step green synthesis of gold conjugated polyphenol nanoparticle using
extracts of Saudi?s myrrh: Their characterization, molecular docking and essential
biological applications
T2 - SAUDI PHARMACEUTICAL JOURNAL
TI - Single-step green synthesis of gold conjugated polyphenol nanoparticle using
extracts of Saudi?s myrrh: Their characterization, molecular docking and essential
biological applications
VL - 30
ID - 6329
ER -
TY - JOUR
AB - An approach to synthesizing silver nanoparticles(AgNPs)using a Fusarium
graminarum fungus was established in the present study.These nanoparticles are
identified with the following techniques to confirm the form, size, and other
physical properties of the crystal:UV-Visible Spectroscopy(UV-Vis),where the AgNPs
showed absorbance at 420 nm, while X-Ray Diffraction (XRD)showed diffraction peaks
at(38.05°,4 nm).The experiment consisted of 40 mice divided into two groups, the
first group of 20 mice was considered to be the control animals and the other group
20mice was 21day of treatment with a dose of AgNPs(0.1ml/ day).Microscopic
examination of the kidney section showed lobulated glomeruli, a large area of
hemorrhage, changes in degeneration, and inflammatory cell infiltration. Biometric
changes of the renal corpuscle showed no significant difference between the study
group, while Bowman’s capsule, Proximal, and distal convoluted tubules revealed
increased significant differences between the two groups. © 2020, World
Informations Syndicate. All rights reserved.
AU - Al-Sharqi, S. A. H.
DB - Scopus
IS - 3
KW - AgNPs
Fusarium graminarum
Image J
Kidney
Toxicity
M3 - Article
PY - 2020
SP - 1029-1035
ST - Histological and biometric study of the effects of fusarium graminarum silver
nanoparticles on the kidney in male albino mice
T2 - Medico-Legal Update
TI - Histological and biometric study of the effects of fusarium graminarum silver
nanoparticles on the kidney in male albino mice
85093113700&partnerID=40&md5=47067abdb9ce6039c40a1dcd9be9de20
VL - 20
ID - 5297
ER -
TY - JOUR
AB - We evaluated the fects of different levels of dietary silver nanoparticle
(AgNP) powder on performance, intestinal microflora, carcass traits and blood
parameters of broiler chickens. Three hundred seven-day-old Ross broiler chicks
were randomly divided into five groups, each group replicated three times with 20
birds per replication. Chickens were fed the basal diet with 2.5, 5, 10 and 20 mg
AgNPs per kg feed. Dietary inclusion of AgNPs improved the final body weight,
cumulative weight gain and feed conversion ratio. The best broiler performance,
carcass traits, and relative organ weights were observed in the group supplemented
with 2.5 ppm AgNPs. Increasing the AgNP dose resulted in a significant decrease in
the caecal lactose positive and enterococci bacteria populations, while
lactobacilli counts were numerically increased. The silver residues in the breast
and thigh muscle significantly increased (p <.05) in a dose-dependent manner.
Dietary inclusion of AgNPs induced dose-dependent lesions in liver, kidney, spleen
and duodenum tissues involving degeneration, necrosis, mononuclear infiltration and
focal aggregation of inflammatory cells. In conclusion, despite its potential
positive impacts on growth performance, carcass traits and caecal microbial
population diversity at a dose of 2.5 ppm, dietary inclusion of AgNPs had the
following negative effects on broilers: 1) silver residues in breast and thigh
muscle, which may result in AgNPs transmission to consumers, and 2) cytotoxicity in
intestinal, liver, spleen and kidney cells in a dose-dependent manner. Therefore,
we suggest the use of lower doses of AgNPs (< 2.5 ppm diet) in poultry production
in the future studies.HIGHLIGHTS Dietary inclusion of silver nanoparticles (AgNPs)
in broiler diets more than 2.5 mg/kg diets had many negative effects represented by
accumulation of silver residue in broiler meat and the possibility of transmission
of nanosilver to consumers. AgNPs had a cytotoxic effect on intestine, liver,
spleen and kidney cells in a dose-dependent manner in broilers and might be harmful
to chicken and human health. Therefore, we do not recommend using AgNPs as a
dietary growth promotor or antibacterial agent in broiler diets and their use and
marketing should be controlled and restricted. © 2022 The Author(s). Published by
Informa UK Limited, trading as Taylor & Francis Group.
AU - Al-Sultan, S. I.
AU - Hereba, A. R. T.
AU - Hassanein, K. M. A.
AU - Abd-Allah, S. M. S.
AU - Mahmoud, U. T.
AU - Abdel-Raheem, S. M.
DB - Scopus
DO - 10.1080/1828051X.2022.2083528
IS - 1
KW - broilers
carcass traits
histopathology
microflora
Nanoparticles
performance
silver
tissue residues
M3 - Article
PY - 2022
SP - 967-978
ST - The impact of dietary inclusion of silver nanoparticles on growth
performance, intestinal morphology, caecal microflora, carcass traits and blood
parameters of broiler chickens
T2 - Italian Journal of Animal Science
TI - The impact of dietary inclusion of silver nanoparticles on growth
performance, intestinal morphology, caecal microflora, carcass traits and blood
parameters of broiler chickens
85131380851&doi=10.1080%2f1828051X.2022.2083528&partnerID=40&md5=13aafc626c6938f2df
af9881ce936ef3
VL - 21
ID - 5072
ER -
TY - JOUR
AB - This study was conducted to examine the possible effects of Ag-NPs
synthesized using the olive leaf extract on histopathology and cytogenetic effct in
mice. A total of thirty albino mice aged two months were divided into five
treatment groups as follows: Group 1: served as control was administrated orally
with 0.3 mg/kg bw of normal saline; Group 2 and 3 were administrated orally with
varying doses of Ag-NPs synthesized on olive leaf extract (10 and 100 mg/kg bw,
respectively) for 30 days; Group 4 and 5 were administrated orally with olive leaf
crude extract (300 and 1000 mg/kg bw, respectively) for 30 days. At the end of
experimental period, detection of possible chromosomal aberrations in blood samples
and histopathology (liver, spleen, kidney, uterus and testes) were carried out.
Statistically significant differences (P <= 0.05) were observed for the chromosomal
aberrations in all Ag-NPs groups compared to control and to crude olive leaf
extract groups. Histopathological study revealed various alterations in internal
organs at high dose of Ag-NPs group including: inflammatory reaction, blood
congestion, degeneration, fibrosis, mononuclear cells lesion and necrosis. Slight
changes were identified at both doses of crude olive extract treated groups. Based
on these results, oral administration of Ag-NPs could cause genotoxic and
inflammatory responses in mice and this could be representing a risk to both
environment and human health.
AN - WOS:000601190700022
AU - Al-taee, E. H.
DO - 10.36103/ijas.v51i5.1155
IS - 5
PY - 2020
SN - 0075-0530
2410-0862
SP - 1448-1457
ST - EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING LEAVES EXTRACT OF OLIVE ON
HISTOPATHOLOGICAL AND CYTOGENETIC EFFECTS IN ALBINO MICE
T2 - IRAQI JOURNAL OF AGRICULTURAL SCIENCES
TI - EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING LEAVES EXTRACT OF OLIVE ON
HISTOPATHOLOGICAL AND CYTOGENETIC EFFECTS IN ALBINO MICE
VL - 51
ID - 6385
ER -
TY - CHAP
AB - Traumatic brain injury (TBI) is a leading cause of mortality and chronic
disability worldwide TBI involves an initial primary phase triggered by an
impactful force to the brain and a subsequent secondary pathological phase. The
secondary phase is characterized by key cellular events such as the release of
calcium ions (Ca2+) and a cascade of inflammatory events such as the impairment of
mitochondrial function, increase in oxidative stress, activation of glial cells,
and impairment of the blood-brain barrier (BBB) causing paracellular leakage. There
is no FDA-approved drug for TBI, but current treatment strategies rely on the
delivery of small and macromolecular therapies to the brain, and these are severely
restricted by the BBB, poor retention, off-target toxicity, and by the complex
pathology of TBI. Therefore, there is a growing need for novel therapeutics for the
diagnosis and treatment of TBI with effective delivery tactics and treatment
paradigms such as nano-engineering nanoparticles (NPs). Nanoparticles sizes range
between 1-100 nm and are engineered to form distinct materials such as lipids,
organic polymers, and silica and metals complexes providing NPs with
characteristics that can mitigate TBI secondary events like BBB breakage,
neuroinflammation, oxidative stress, and mitochondrial dysfunction, leading to
mitigation of TBI pathology. Limitations of NP technology in the treatment of TBI
are related to bioavailability, toxicity load and proinflammatory activity of NPs
in the brain. In this chapter, we discuss nanoparticles (NPs) as novel strategies
for the treatment of TBI and explore their synthesis, mechanisms of action, and
limitations. Understanding the mechanisms and complications of NPs as novel
therapeutic strategies will help guide and improve the design of future TBI
therapies. © 2023 John Wiley & Sons Ltd.
AU - Al-Thani, N.
AU - Haider, M. Z.
AU - Al-Mansoob, M.
AU - Patel, S.
AU - Ahmad, S. M. S.
AU - Kobeissy, F.
AU - Shaito, A.
DB - Scopus
DO - 10.1002/9781119896258.ch8
KW - Carbon dots
Carbon nanotubules
Fullerenes
Gold nanoparticles
Lipid-based nanoparticles
Nanoparticles and brain injury
Oxidative stress
Polymeric nanoparticles
Reactive oxygen species (ROS)
Traumatic brain injury
PY - 2023
SP - 217-228
ST - Nano-engineering in traumatic brain injury
T2 - Impact of Engineered Nanomaterials in Genomics and Epigenomics
TI - Nano-engineering in traumatic brain injury
85160979667&doi=10.1002%2f9781119896258.ch8&partnerID=40&md5=9a1d6d326390c324e54813
d92ace129f
ID - 5023
ER -
TY - JOUR
AB - Polymerization of deoxy sickle cell hemoglobin (HbS) is well recognized as
the primary event that triggers the classic cycles of sickling/unsickling of
patients red blood cells (RBCs). RBCs are also subjected to continuous endogenous
and exogenous oxidative onslaughts resulting in hemolytic rate increases which
contribute to the evolution of vasculopathies associated with this disease.
Compared to steady-state conditions, the occurrences of vaso-occlusive crises
increase the levels of both RBC-derived microparticles as well as extracellular Hb
in circulation. Common byproduct resulting from free Hb oxidation and from Hb-laden
microparticles is heme (now recognized as damage associated molecular pattern
(DAMP) molecule) which has been shown to initiate inflammatory responses. This
review provides new insights into the interplay between microparticles, free Hb and
heme focusing on Hb's pseudoperoxidative activity that drives RBC's cytosolic,
membrane changes as well as oxidative toxicity towards the vascular system.
Emerging antioxidative strategies that include the use of protein and heme
scavengers in controlling Hb oxidative pathways are discussed.
AN - WOS:000430393500007
AU - Alayash, A. I.
DA - MAY
DO - 10.1016/j.bcmd.2017.05.009
PY - 2018
SN - 1079-9796
1096-0961
SP - 78-86
ST - Oxidative pathways in the sickle cell and beyond
T2 - BLOOD CELLS MOLECULES AND DISEASES
TI - Oxidative pathways in the sickle cell and beyond
VL - 70
ID - 6502
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are increasingly being incorporated into
products for their antimicrobial properties. This has resulted in increased human
exposures and the possibility of adverse health effects. Mast cells orchestrate
allergic immune responses through degranulation and release of pre-formed
mediators. Little data exists on understanding interactions of AgNPs with mast
cells and the properties that influence activation and degranulation. Using bone
marrow-derived mast cells and AgNPs of varying physicochemical properties we tested
the hypothesis that AgNP physicochemical properties influence mast cell
degranulation and osteopontin production. AgNPs evaluated included spherical 20 nm
and 110 nm suspended in either polyvinylpyrrolidone (PVP) or citrate, Ag plates
suspended in PVP of diameters between 40-60 nm or 100-130 nm, and Ag nanowires
suspended in PVP with thicknesses <100 nm and length up to 2 mu m. Mast cell
responses were found to be dependent on the physicochemical properties of the AgNP.
Further, we determined a role for scavenger receptor B1 in AgNP-induced mast cell
respons8. Mast cell degranulation was not dependent on AgNP dissolution but was
prevented by tyrosine lcinase inhibitor pretreatment. This study suggests that
exposure to AgNPs may elicit adverse mast cell responses that could contribute to
the initiation or exacerbation of allergic disease. (C) 2014 Elsevier Ltd. All
rights reserved.
AN - WOS:000347746600026
AU - Aldossari, A. A.
AU - Shannahana, J. H.
AU - Podila, R.
AU - Brown, J. M.
DA - FEB
DO - 10.1016/j.tiv.2014.10.008
IS - 1
PY - 2015
SN - 0887-2333
SP - 195-203
ST - Influence of physicochemical properties of silver nanoparticles on mast cell
activation and degranulation
T2 - TOXICOLOGY IN VITRO
TI - Influence of physicochemical properties of silver nanoparticles on mast cell
activation and degranulation
VL - 29
ID - 5953
ER -
TY - JOUR
AB - The bio-based nanoparticles synthesis and assessment of their potential
biomedical applications related research is rapidly emerging. The ability of an
aqueous ethanolic bark extract of Mangifera indica to synthesize silver
nanoparticles (AgNPs) as well as its antibacterial, anti-inflammatory, and
anticancer activities were investigated in this study. Interestingly, the bark
extract effectively synthesized the AgNPs, including an absorbance peak at 412 nm
and sizes ranging from 56 to 89 nm. The Fourier Transform Infrared spectroscopy
(FTIR) analysis confirmed that the presence of most essential functional groups
belongs to the most bioactive compounds. Synthesized AgNPs showed fine
antibacterial activity against the Urinary Tract Infection (UTI) causing bacterial
pathogens such as Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae,
Proteus mirabilis, and Staphylococcus saprophyticus at 50 μg mL−1 concentrations.
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration
(MBC) of AgNPs against these pathogens were found as 12.5 ± 0.8 & 13 ± 0.6, 13.6 ±
0.5 & 14 ± 0.7, 11.5 ± 0.3 & 11.5 ± 0.4, 13 ± 0.8 & 13 ± 0.7, and 11.8 ± 0.4 & 12 ±
0.8 μg mL−1 respectively. Interestingly, this AgNPs also possesses outstanding
anti-inflammatory and anticancer activities as studied against the egg albumin
denaturation (85%) inhibition and MCF 7 (Michigan Cancer Foundation-7: breast
cancer cells) cell line (cytotoxicity: 80.1%) at 50 μg mL−1 concentration.
Similarly at 50 μg mL−1 concentration showed 75% of DPPH radical scavenging
potential. These activities were dose dependent, and the findings suggest that the
M. indica bark aqueous ethanolic extract synthesized AgNPs can be used as
antibacterial, anti-inflammatory, and anticancer agents after in-vivo testing. ©
2023 Elsevier Inc.
AU - Algarni, A.
AU - Fayomi, A.
AU - Al Garalleh, H.
AU - Afandi, A.
AU - Brindhadevi, K.
AU - Pugazhendhi, A.
C7 - 115983
DB - Scopus
DO - 10.1016/j.envres.2023.115983
Antibacterial
Anticancer
Aqueous ethanolic extract
Mangifera indica
Anticoagulants
Metal Nanoparticles
Silver
Michigan
United States
chloramphenicol
Mangifera indica extract
silver nanoparticle
anticoagulant agent
antiinfective agent
metal nanoparticle
silver
anticoagulant
bacterial disease
bark
cancer
inhibition
anticoagulation
Article
controlled study
DPPH radical scavenging assay
drug synthesis
Enterococcus faecalis
Escherichia coli
in vitro study
Klebsiella pneumoniae
mango
MCF-7 cell line
minimum bactericidal concentration
nanofabrication
nonhuman
particle size
Proteus mirabilis
Staphylococcus saprophyticus
chemistry
M3 - Article
PY - 2023
ST - Nanofabrication synthesis and its role in antibacterial, anti-inflammatory,
and anticoagulant activities of AgNPs synthesized by Mangifera indica bark extract
T2 - Environmental Research
TI - Nanofabrication synthesis and its role in antibacterial, anti-inflammatory,
and anticoagulant activities of AgNPs synthesized by Mangifera indica bark extract
85158014937&doi=10.1016%2fj.envres.2023.115983&partnerID=40&md5=a9eba47ffe9f58ca33e
880265ad43365
VL - 231
ID - 5015
ER -
TY - JOUR
AB - Objective. Multinucleated cells are relatively resistant to classic
apoptosis, and the factors initiating cell death and damage in myositis are not
well defined. We hypothesized that nonimmune autophagic cell death may play a role
in muscle fiber damage. Recent reports indicate that TRAIL may induce both NF-kappa
B activation and autophagic cell death in other systems. We undertook this study to
investigate the role of TRAIL in cell death and pathogenesis in vitro and in vivo,
using myositis muscle tissues from humans and mice. Methods. Gene expression
profiling was performed in myositis patient and control muscle specimens.
Immunohistochemistry analysis was performed to confirm the gene array findings. We
also analyzed TRAIL-induced cell death (apoptosis and autophagy) and NF-kappa B
activation in vitro in cultured cells. Results. TRAIL was expressed predominantly
in myositis muscle fibers, but not in biopsy specimens from normal or other
dystrophic-diseased muscle. Autophagy markers were up-regulated in humans with
myositis and in mouse models of myositis. TRAIL expression was restricted to
regenerating/atrophic areas of muscle fascicles, blood vessels, and infiltrating
lymphocytes. TRAIL induced NF-kappa B activation and I kappa B degradation in
cultured cells that are resistant to TRAIL-induced apoptosis but that undergo
autophagic cell death. Conclusion. Our data demonstrate that TRAIL is expressed in
myositis muscle and may mediate both activation of NF-kappa B and autophagic cell
death in myositis. Thus, this nonimmune pathway may be an attractive target for
therapeutic intervention in myositis.
AN - WOS:000297221100029
AU - Alger, H. M.
AU - Raben, N.
AU - Pistilli, E.
AU - Francia, D. L.
AU - Rawat, R.
AU - Getnet, D.
AU - Ghimbovschi, S.
AU - Chen, Y. W.
AU - Lundberg, I. E.
AU - Nagaraju, K.
DA - NOV
DO - 10.1002/art.30530
IS - 11
PY - 2011
SN - 0004-3591
SP - 3448-3457
ST - The Role of TRAIL in Mediating Autophagy in Myositis Skeletal Muscle A
Potential Nonimmune Mechanism of Muscle Damage
T2 - ARTHRITIS AND RHEUMATISM
TI - The Role of TRAIL in Mediating Autophagy in Myositis Skeletal Muscle A
Potential Nonimmune Mechanism of Muscle Damage
VL - 63
ID - 6731
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are gaining interest in medical applications for
their prominent antibacterial and antimicrobial potentials. AgNPs possess
remarkable anti-inflammatory and antioxidant activities and enhances wound healing.
The main objective of the current study was to investigate the therapeutic effect
of administration of AgNPs on cisplatin (CP) induced pulmonary inflammation in
rats. Sixty male albino rats were used in this study. Rats were divided into 6
groups (n=10). Group I control group. Group II and III control groups received
AgNPs at doses (5 and 10 ppm). Group IV CP group received CP (2.5 mg/kg). Group V
and VI CP group received AgNPs (5, and 10 ppm). All doses were administered
intraperitoneally once a day for 4 weeks. Oxidative stress and antioxidant status,
inflammatory mediators, fibrogenic as well as apoptotic markers were determined in
lung tissues. The results revealed that rats treated with CP showed remarkable
elevation in lung tissues MDA, TNF-alpha, IFN-gamma, IL-6, CRP, Fibrinogen and P53
levels associated with depression in SOD, GSH and CAT activities. However,
administration of AgNPs (5 or 10 ppm) to CP group resulted in significant
amelioration of the aforementioned parameters in a dose dependent manner.
Histopathological investigation of lung tissues of CP group demonstrated disruption
of normal lung architecture and lung injury. However, treatment with AgNPs revealed
significant improvement in lung tissue against CP-induced inflammatory changes and
lung tissue damage. It could be concluded that AgNPs exert potent cytoprotective
effects via combating oxidative stress, inflammation, fibrogenic and apoptotic
markers and repairing histopathological changes in lung tissues.
AN - WOS:000708638800044
AU - Alharbi, H. Y.
AU - Helmi, N. W.
AU - Salem, N. A.
DO - 10.9734/JPRI/2021/v33i46A32888
IS - 46A
PY - 2021
SN - 2456-9119
SP - 453-463
ST - Possible Protective Effect of Silver Nanoparticles against Cisplatin Induced
Pulmonary Inflammation in Rat Model
T2 - JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL
TI - Possible Protective Effect of Silver Nanoparticles against Cisplatin Induced
Pulmonary Inflammation in Rat Model
VL - 33
ID - 5955
ER -
TY - JOUR
AB - Nanomaterials are utilized in a wide array of end user products such as
pharmaceuticals, electronics, clothes and cosmetic products. Due to its size (<100
nm), nanoparticles have the propensity to enter through the airway and skin, making
its path perilous with the potential to cause damages of varying severity. Once
within the body, these particles have unconstrained access to different tissues and
organs including the brain, liver, and kidney. As a result, nanomaterials may cause
the perturbation of the immune system eliciting an inflammatory response and
cytotoxicity. This potential role is dependent on many factors such as the
characteristics of the nanomaterials, presence or absence of diseases, and genetic
predisposition. Cobalt and nickel nanoparticles, for example, were shown to have
inflammogenic properties, while silver nanoparticles were shown to reduce allergic
inflammation. Just as asbestos fibers, carbon nanotubes were shown to cause lungs
damage. Some nanomaterials were shown, based on animal studies, to result in cell
damage, leading to the formation of pre-cancerous lesions. This review highlights
the impact of nanomaterials on immune system and its effect on human health with
toxicity consideration. It recommends the development of suitable animal models to
study the toxicity and bio-clearance of nanomaterials and propose safety
guidelines. Copyright © 2016 American Scientific Publishers.
AU - Ali, A.
AU - Suhail, M.
AU - Mathew, S.
AU - Shah, M. A.
AU - Harakeh, S. M.
AU - Ahmad, S.
AU - Kazmi, Z.
AU - Alhamdan, M. A. R.
AU - Chaudhary, A.
AU - Damanhouri, G. A.
AU - Qadri, I.
DB - Scopus
DO - 10.1166/jnn.2016.10885
IS - 1
KW - Apoptosis
Autophagy
Cellular uptake
Cytochrome p450
Inflammation
Macrophages
Nanomaterials
Nanoparticles
Nanotoxicology
Necrosis
Pyroptosis
Adaptive Immunity
Animals
Asbestos
Cytotoxins
Humans
Lung Injury
Metal Nanoparticles
Metals
Nanotubes, Carbon
Carbon
Cell death
Immune system
Pathology
Silver
Toxicity
Yarn
asbestos
carbon nanotube
cytotoxin
metal
metal nanoparticle
adaptive immunity
animal
chemically induced
drug effects
human
immunology
lung injury
pathology
M3 - Review
PY - 2016
SP - 40-57
ST - Nanomaterial induced immune responses and cytotoxicity
T2 - Journal of Nanoscience and Nanotechnology
TI - Nanomaterial induced immune responses and cytotoxicity
84959353064&doi=10.1166%2fjnn.2016.10885&partnerID=40&md5=ce6b031ae0187f5b67b62a5d5
2293956
VL - 16
ID - 5595
ER -
TY - JOUR
AB - Aspergillus fumigatus is one of the most common fungal pathogens that can
cause a diver-sity of diseases ranging from invasive pulmonary aspergillosis (IPA)
and aspergilloma to allergic syndromes. In this study, we investigated the
antifungal effect of silver nanoparticles biosynthe-sized with Artemisia sieberi
leaf extract (AS-AgNPs) against A. fumigatus in vitro and in vivo. The
biosynthesized AS-AgNPs were characterized by imaging (transmission electron
microscopy (TEM)), UV−VIS spectroscopy, X-ray diffraction (XRD), and Fourier
transform infrared spectroscopy (FTIR). The microdilution method showed the
antifungal activity of AS-AgNPs against A. fumigatus, with an MIC of 128 µg/mL. AS-
AgNPs significantly inhibited the growth of hyphae in all directions, as imaged by
SEM. Additionally, TEM on biofilm revealed invaginations of the cell membrane, a
change in the vacuolar system, and the presence of multilamellar bodies within
vacuoles. Interestingly, AS-AgNPs displayed low cytotoxicity on the A549 human lung
cell line in vitro. Treatment of an invasive pulmonary aspergillosis (IPA) mouse
model with AS-AgNPs demonstrated the potency of AS-AgNPs to significantly reduce
lung tissue damage and to suppress the elevated levels of pro-inflammatory
cytokines, tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1), and
interleukin-17 (IL-17). The therapeutic potential of AS-AgNPs was found to be due
to their direct action to suppress the fungal burden and gliotoxin production in
the lungs. In addition, AS-AgNPs reduced the oxidative stress in the lungs by
increasing the enzymatic activities of catalase (CAT) and superoxide dismutase
(SOD). Thus, our data indicate the biosynthesized AS-AgNPs as a novel antifungal
alternative treatment against aspergillosis. © 2021 by the authors. Licensee MDPI,
Basel, Switzerland.
AU - Ali, E. M.
C7 - 51
DB - Scopus
DO - 10.3390/nano12010051
IS - 1
KW - Antifungal
Aspergillus fumigatus
Calotropis gigantea
Invasive pulmonary aspergillosis
M3 - Article
PY - 2022
ST - Effective inhibition of invasive pulmonary aspergillosis by silver
nanoparticles biosynthesized with artemisia sieberi leaf extract
T2 - Nanomaterials
TI - Effective inhibition of invasive pulmonary aspergillosis by silver
nanoparticles biosynthesized with artemisia sieberi leaf extract
85121634607&doi=10.3390%2fnano12010051&partnerID=40&md5=551864f05dd8c1a8af30cfee70c
feb3b
VL - 12
ID - 5104
ER -
TY - JOUR
AB - Context: Nanotechnology is widely used nowadays in several fields of
industry, engineering, and medicine, the biological action mechanisms of AgNPs,
which mainly involve the release of silver ions (Ag+), generation of reactive
oxygen species (ROS). Objective: The potential toxicity AgNPs of damages to hepatic
cells, hesperidin, and naringin role for their protective effect against the
increase of ROS due to AgNPs toxicity. They can be restored, most cellular
biochemical parameters, genotoxicity, mutagenicity, and histopathological analysis.
Materials and methods: Toxicity was induced by an oral dose of Ag NPs of (20–100
nm) for one month, after that treated with hesperidin, naringin (100 mg/kg) for
three weeks, malondialdehyde (MDA) levels, nitric oxide (NO), glutathione (GSH) and
catalase were estimated. Also, aminotransferases (AST and ALT), alkaline
phosphatase (ALP), γ-glutamyltransferase (GGT), albumin, and total bilirubin were
determined, following Chromosomal aberrations, DNA breaks, and histological
analyses Results: hesperidin, and naringin treatment, recorded amelioration in most
biochemical, genetic, and spermatogenesis disturbances Also, histological
Investigations were improved. Conclusion: Their biological safety problems, such as
potential toxicity on cells, tissue, and organs should be paid enough attention,
hesperidin and naringin amelioration fundamental alterations, as hepatic
architectural and DNA damage, related to its role as an antioxidant and anti-
inflammatory agent. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
AU - Ali, S. A.
AU - Gooda, S. M.
AU - Aboul Naser, A. F.
AU - Younis, E. A.
AU - Hamed, M. A.
AU - Ahmed, Y. R.
AU - Farghaly, A. A.
AU - Khalil, W. K. B.
AU - Rizk, M. Z.
DB - Scopus
DO - 10.1080/1354750X.2022.2046856
IS - 4
KW - citrus flavonoids
DNA lesions
histopathology
liver function
oxidative stress
Animals
Chromosome Aberrations
DNA Damage
Glutathione
Hesperidin
Humans
Liver
Male
Metal Nanoparticles
Mice
Oxidative Stress
Particle Size
Silver
albumin
aminotransferase
aurantiin
bilirubin
bioflavonoid
catalase
gamma glutamyltransferase
glutathione
hesperidin
malonaldehyde
natural product
nitric oxide
silver nanoparticle
metal nanoparticle
silver
animal cell
animal experiment
Article
aspartate aminotransferase level
bone marrow cell
chemical structure
chromosome aberration
comet assay
controlled study
cytogenetic analysis
DNA damage
DNA determination
DNA strand breakage
drug effect
drug safety
grapefruit
liver tissue
male
mouse
nonhuman
particle size
relative humidity
room temperature
sour orange
spermatogenesis
animal
human
liver
metabolism
M3 - Article
PY - 2022
SP - 349-360
ST - Chromosomal aberrations, DNA damage, and biochemical disturbances induced by
silver nanoparticles in mice: role of particle size and natural compounds treatment
T2 - Biomarkers
TI - Chromosomal aberrations, DNA damage, and biochemical disturbances induced by
85126706950&doi=10.1080%2f1354750X.2022.2046856&partnerID=40&md5=42086ba43b86aeffdf
ccaa1ff125a92a
VL - 27
ID - 5089
ER -
TY - JOUR
AB - Context Nanotechnology is widely used nowadays in several fields of industry,
engineering, and medicine, the biological action mechanisms of AgNPs, which mainly
involve the release of silver ions (Ag+), generation of reactive oxygen species
(ROS). Objective The potential toxicity AgNPs of damages to hepatic cells,
hesperidin, and naringin role for their protective effect against the increase of
ROS due to AgNPs toxicity. They can be restored, most cellular biochemical
parameters, genotoxicity, mutagenicity, and histopathological analysis. Materials
and methods Toxicity was induced by an oral dose of Ag NPs of (20-100 nm) for one
month, after that treated with hesperidin, naringin (100 mg/kg) for three weeks,
malondialdehyde (MDA) levels, nitric oxide (NO), glutathione (GSH) and catalase
were estimated. Also, aminotransferases (AST and ALT), alkaline phosphatase (ALP),
gamma-glutamyltransferase (GGT), albumin, and total bilirubin were determined,
following Chromosomal aberrations, DNA breaks, and histological analyses Results
hesperidin, and naringin treatment, recorded amelioration in most biochemical,
genetic, and spermatogenesis disturbances Also, histological Investigations were
improved. Conclusion Their biological safety problems, such as potential toxicity
on cells, tissue, and organs should be paid enough attention, hesperidin and
naringin amelioration fundamental alterations, as hepatic architectural and DNA
damage, related to its role as an antioxidant and anti-inflammatory agent.
AN - WOS:000770256600001
AU - Ali, S. A.
AU - Gooda, S. M.
AU - Naser, A. F. A.
AU - Younis, E. A.
AU - Hamed, M. A.
AU - Ahmed, Y. R.
AU - Farghaly, A. A.
AU - Khalil, W. K. B.
AU - Rizk, M. Z.
C6 - MAR 2022
DA - MAY 19
DO - 10.1080/1354750X.2022.2046856
IS - 4
PY - 2022
SN - 1354-750X
1366-5804
SP - 349-360
ST - Chromosomal aberrations, DNA damage, and biochemical disturbances induced by
T2 - BIOMARKERS
TI - Chromosomal aberrations, DNA damage, and biochemical disturbances induced by
VL - 27
ID - 6047
ER -
TY - JOUR
AB - We report that the immunogenicity of colloidal gold nanoparticles coated with
polyvinylpyrrolidone (PVP-AuNPs) in a model organism, the sea urchin Paracentrotus
lividus, can function as a proxy for humans for in vitro immunological studies. To
profile the immune recognition and interaction from exposure to PVP-AuNPs (1 and 10
mu g mL-1), we applied an extensive nano-scale approach, including particle
physicochemical character-isation involving immunology, cellular biology, and
metabolomics. The interaction between PVP-AuNPs and soluble proteins of the sea
urchin physiological coelomic fluid (blood equivalent) results in the formation of
a protein "corona" surrounding the NPs from three major proteins that influence the
hydrodynamic size and colloidal stability of the particle. At the lower
concentration of PVP-AuNPs, the P. lividus phagocytes show a broad metabolic
plasticity based on the biosynthesis of metabolites mediating inflammation and
phagocytosis. At the higher concentration of PVP-AuNPs, phagocytes activate an
immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at
24 hours of exposure. These results emphasise that exposure to PVP-AuNPs drives
inflammatory signalling by the phagocytes and the resolution at both the low and
high concentrations of the PVP-AuNPs and provides more details regarding the
immunogenicity of these NPs.
AN - WOS:000593840400007
AU - Alijagic, A.
AU - Barbero, F.
AU - Gaglio, D.
AU - Napodano, E.
AU - Benada, O.
AU - Kofronova, O.
AU - Puntes, V. F.
AU - Bastus, N. G.
AU - Pinsino, A.
DA - JAN 15
PY - 2021
SN - 0304-3894
1873-3336
ST - Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin
extracellular molecules induce transient immune activation
T2 - JOURNAL OF HAZARDOUS MATERIALS
TI - Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin
extracellular molecules induce transient immune activation
VL - 402
ID - 6803
ER -
TY - JOUR
AB - Indole-3-carbinol (I3C) is a plant based compound present in vegetables
mostly belonging to cruciferous family. I3C has been shown to possess anticancer,
antioxidant and antiinflammatory properties. Humans are increasingly being exposed
to GNPs due an due to their widespread and increased applications in different
fields. Consistently, recent animal and cell based studies have found them to be
carcinogenic, prooxidant and inflammatory. This study sought to examine the
beneficial effects of I3C against oxidative stress, inflammation and
histopathological changes in liver tissues of rats administered with GNPs. Forty
rats randomly divided into four groups: G1 control G2, rats injected i.p. with a
suspension of GNPs (10 nm in size) (20 mu g/kg body wgt) for 7 days; G3, rats
supplemented orally with I3C (150 mg/kg body wgt) for 7 days; and G4, rats injected
with GNPs along with oral supplementation of I3C. Compared to control, rats
administered with GNPs had significantly increased liver functional markers
including ALT, AST, ALP, total and direct bilirubin and significantly decreased
albumin levels. GNP administered rats also demonstrated increased oxidative stress
and inflammatory markers, MDA, 8-OHdG and IL-6 levels and significantly depleted
activities of antioxidants such as glutathione reductase (GR) and glutathione S-
transferase (GST), and transcription factor, Nrf2 compared to control. GNPs also
exerted marked histological changes in liver tissues. Treatment with I3C
significantly restored the GNP induced changes in the levels of all the studied
parameters and also prevented pathological changes in liver tissues. Molecular
docking studies confirm the interaction of I3C with hepatic cell surface receptor
protein LT3 and thereby blocking GNPs from binding to I3C, confirming above
experimental findings. Collectively, the data demonstrate the hepatoprotective
effects of I3C against GNP-induced adverse effects on liver tissue. These
protective effects of I3C appear to be mediated by its ability to downregulate
oxidative stress and inflammation. Thus dietary intake of plant products rich in
I3C may have beneficial health effects. (C) 2020 Published by Elsevier B.V. on
behalf of King Saud University.
AN - WOS:000583851900036
AU - Alkhalaf, M. I.
DA - NOV
DO - 10.1016/j.arabjc.2020.09.035
IS - 11
PY - 2020
SN - 1878-5352
1878-5379
SP - 8060-8068
ST - Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced
hepatotoxicity in rats
T2 - ARABIAN JOURNAL OF CHEMISTRY
TI - Attenuating effect of Indole-3-Carbinol on gold nanoparticle induced
hepatotoxicity in rats
VL - 13
ID - 6699
ER -
TY - JOUR
AB - Green synthesis of silver nanoparticles has gained great interest among
scientists. In view of this data, we conducted this study to identify the
ameliorative effect of green synthesis of silver nanoparticles using Nigella sativa
extract in diabetic neuropathy induced experimentally. In this study, 50 adult male
albino rats were used and they were randomly divided into five groups; the first
group was the healthy control group, the second group were the diabetic neuropathy
diabetic neuropathy induced, Groups (3-6) diabetic neuropathy induced group and
treated with silver nanoparticles, Nigella sativa extract and green synthesized
silver nanoparticles using Nigella sativa extract respectively. Biochemical
parameters including diabetic, inflammatory and antioxidant biomarkers were
evaluated. Brain histopathology was also performed. Results revealed substantial
rise in glucose, AGE, aldose reductase with insulin reduction in diabetic
neuropathy induced group as compared to healthy control. Also, inflammatory markers
increased significantly in diabetic neuropathy induced group. A remarkable change
in oxidative status was observed in the same group. Furthermore, significant
decline in nitrotyrosin level was observed. Regarding gene expression, we found
significant down regulation in brain TKr A accompanied by upregulation of nerve
growth factor in diabetic neuropathy group comparing with healthy control. Several
treatments for diabetic neuropathy remarkably ameliorate all the investigated
biomarkers. Histological findings are greatly relied on for the results achieved in
this study. Therefore, it can be established that green synthesis of silver
nanoparticles in combination with Nigella sativa extract could be a newly
neuroprotective agents against inflammation and oxidative stress characterizing
diabetic neuropathy through their antidiabetic, anti-inflammatory and anti-oxidants
effects. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of King Saud
University.
AN - WOS:000559926000006
AU - Alkhalaf, M. I.
AU - Hussein, R. H.
AU - Hamza, A.
DA - SEP
DO - 10.1016/j.sjbs.2020.05.005
IS - 9
PY - 2020
SN - 1319-562X
2213-7106
SP - 2410-2419
ST - Green synthesis of silver nanoparticles by Nigella sativa extract alleviates
diabetic neuropathy through anti-inflammatory and antioxidant effects
TI - Green synthesis of silver nanoparticles by Nigella sativa extract alleviates
diabetic neuropathy through anti-inflammatory and antioxidant effects
VL - 27
ID - 5860
ER -
TY - JOUR
AB - Nanosilver is widely used in medicine, industry, and other applications where
it poses a high potential risk for human health, even though little information is
available on its toxicity to vital organs. This study was conducted to determine
the histopathological changes induced in renal tissues by various sizes of silver
nanoparticles (SNPs). Male BALB/C mice were exposed to various sizes of SNPs for 5
weeks. Renal tissue samples from all members of all experimental groups were
subjected to histological processing and histopathological examination. SNPs caused
glomerular and tubular alterations in the form of tubular degeneration, necrosis,
eosinophilia, glomerular shrinkage, and Bowman's capsule thickening. Moreover, SNPs
induced interstitial intertubular regeneration, mononuclear inflammatory cell
infiltration, proteinaceous casting, and fibrocyte proliferation. These results
indicated that smaller particles (10 and 20 nm) were more toxic than the larger
ones (40, 60, and 100 nm). In addition, the cortex was more affected than the
medulla, and the proximal tubules were more affected than the distal ones. The
results of the current investigation reveal that SNPs induce histomorphological
alterations in renal tissues, with size being a key factor in the toxicity of these
particles. © 2016 The Institution of Engineering and Technology.
AU - Almansour, M.
AU - Jarrar, Q.
AU - Battah, A.
AU - Obeidat, F.
AU - Battah, K.
AU - Jarrar, B.
DB - Scopus
DO - 10.1049/mnl.2016.0388
IS - 12
KW - Health risks
Histology
Silver
Tissue
silver nanoparticle
Experimental groups
High potential
Histopathological examinations
Inflammatory cells
Proximal tubules
Silver nanoparticles (SNPs)
Size effects
Tubular alterations
animal experiment
animal model
animal tissue
Article
Bowman capsule
cell infiltration
cell proliferation
controlled study
eosinophilia
histopathology
kidney parenchyma
male
mouse
necrosis
nonhuman
Toxicity
M3 - Article
PY - 2016
SP - 862-865
ST - Renal histopathological alterations induced by nanosilver toxicity: The size
effect
T2 - Micro and Nano Letters
TI - Renal histopathological alterations induced by nanosilver toxicity: The size
effect
84999040197&doi=10.1049%2fmnl.2016.0388&partnerID=40&md5=fa64f104bd4c2c86c8def6e411
a299b2
VL - 11
ID - 5519
ER -
TY - JOUR
AB - Silver nanoparticles (SNPs) are widely invested in nanomedicine and consuming
products due to their unique antimicrobial properties. However, little is known
about the toxicity of these particles on human health. The present investigation
was carried out to investigate the histological alterations induced in the lung
tissues by 20±5 nm SNPs. Male albino Wistar rats were exposed to SNPs at a daily
dose of 2 mg/kg for 21 days. Lung biopsies from all rats under study were subjected
to histopathological examinations. Exposure to 20±5 nm SNPs induced the following
pulmonary alterations: thickened alveolar wall, macrophages invasion and
inflammatory cells infiltration, lymphatic follicles enlargement, pulmonary edema,
alveolar hypersensitivity and interstitial congestion. Occasional atelectasis and
fibrocytes proliferation were also detected. The findings of the present work might
indicate that SNPs potentially trigger oxidative stress and alterations in the
pulmonary tissues that may affect the function of the lungs. © (2016) Trans Tech
Publications, Switzerland.
AU - Almansour, M.
AU - Sajti, C. L.
AU - Shraideh, Z.
AU - Jarrar, B.
DB - Scopus
DO - 10.4028/www.scientific.net/JNanoR.35.104
KW - Alveolar wall
Atelectasis
Lung
Nanotoxicity
Pulmonary tissues
Biological organs
Histology
Medical nanotechnology
Metal nanoparticles
Nanoparticles
Rats
Tissue
Silver
M3 - Article
PY - 2015
SP - 104-114
ST - Pulmonary histological alterations induced by 20 nm silver nanoparticles
T2 - Journal of Nano Research
TI - Pulmonary histological alterations induced by 20 nm silver nanoparticles
84945175371&doi=10.4028%2fwww.scientific.net
%2fJNanoR.35.104&partnerID=40&md5=a47a4ed4214332787d9c860951d789cf
VL - 35
ID - 5619
ER -
TY - JOUR
AB - Silver nanoparticles (SNPs) are widely invested in nanomedicine and consuming
products due to their unique antimicrobial properties. However, little is known
about the toxicity of these particles on human health. The present investigation
was carried out to investigate the histological alterations induced in the lung
tissues by 20 +/- 5 nm SNPs. Male albino Wistar rats were exposed to SNPs at a
daily dose of 2 mg/kg for 21 days. Lung biopsies from all rats under study were
subjected to histopathological examinations. Exposure to 20 +/- 5 nm SNPs induced
the following pulmonary alterations: thickened alveolar wall, macrophages invasion
and inflammatory cells infiltration, lymphatic follicles enlargement, pulmonary
edema, alveolar hypersensitivity and interstitial congestion. Occasional
atelectasis and fibrocytes proliferation were also detected. The findings of the
present work might indicate that SNPs potentially trigger oxidative stress and
alterations in the pulmonary tissues that may affect the function of the lungs.
AN - WOS:000373960200010
AU - Almansour, M.
AU - Sajti, C. L.
AU - Shraideh, Z.
AU - Jarrar, B.
DO - 10.4028/www.scientific.net/JNanoR.35.104
PY - 2016
SN - 1662-5250
1661-9897
SP - 104-114
ST - Pulmonary Histological Alterations Induced by 20 nm Silver Nanoparticles
T2 - JOURNAL OF NANO RESEARCH
TI - Pulmonary Histological Alterations Induced by 20 nm Silver Nanoparticles
VL - 35
ID - 5897
ER -
TY - JOUR
AB - Aim To evaluate bone tissue reactions in rats to an MTA-based endodontic
sealer with and without the addition of various concentrations of C3A or C3A + Ag.
Methodology Bone tissue reactions were evaluated in 45 Wistar rats after 7, 30 and
90 days (n = 5 per period). Three surgical cavities were prepared on the right
femur and filled with 0.2 mL MTA Fillapex, MTA Fillapex + C3A and C3A + Ag at
various concentrations: AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany),
EndoSequence BC (Brasseler USA, Savannah, GA, USA) or no sealer (negative control).
By the end of each experimental period, animals were randomly euthanized. The
samples were histologically processed and analysed using a light microscope. The
presence of inflammatory cells, fibres and hard tissue barrier formation was
evaluated. Data were analysed statistically using nonparametric tests to compare
the differences between groups. Multiple groups were compared using the Kruskal-
Wallis and Mann-Whitney U-tests with a Bonferroni correction at P = 0.05. Results
The inflammatory response significantly decreased from 30 to 90 days (P < 0.05).
Fibre condensation was similar amongst the groups at 07 and 30 days after
intervention (P > 0.05). At 90 days, however, fibres were absent in most specimens
of EndoSequence BC Sealer, AH Plus, MTA Fillapex and the control group, whilst they
were still observed in samples of the modified sealers (P < 0.05). At 90 days, all
specimens of AH Plus, EndoSequence BC Sealer and control group had complete
formation of hard tissue barrier. In the MTA Fillapex group, as well as in the
modified sealers groups, partial deposition of mineralized tissue was noticed.
Conclusion The hypothesis tested that the incorporation of C3A and C3A + Ag
particles to MTA Fillapex would improve bone tissue repair was partially accepted,
since modified MTA Fillapex did not have the same repair potential as the
commercial bioceramic material.
AN - WOS:000485318200006
AU - Almeida, L. H.
AU - Gomes, A. P. N.
AU - Gastmann, A. H.
AU - Pole, N. M.
AU - Moraes, R. R.
AU - Morgental, R. D.
AU - Cava, S. S.
AU - Felix, A. O. C.
AU - Peppen, F. G.
DA - OCT
DO - 10.1111/iej.13135
IS - 10
PY - 2019
SN - 0143-2885
1365-2591
SP - 1446-1456
ST - Bone tissue response to an MTA-based endodontic sealer, and the effect of the
addition of calcium aluminate and silver particles
T2 - INTERNATIONAL ENDODONTIC JOURNAL
TI - Bone tissue response to an MTA-based endodontic sealer, and the effect of the
addition of calcium aluminate and silver particles
VL - 52
ID - 6460
ER -
TY - JOUR
AB - Recent research has shown that latex from different species is able to
produce tissue replacement and regeneration. Particularly, biomembranes obtained
from Hancornia speciosa latex (HSB) have shown high angiogenic and osteogenic
activity. Considering new materials for wound healing, it would be interesting to
develop a product combining antibacterial and antifungal activities. Silver
nanoparticles (AgNP) have been commonly used for this purpose in medicinal products
and devices for decades. In order to combine angiogenic, antibacterial and
antifungal properties on the same platform, we developed an HSB containing 3
concentrations of AgNP. It was observed that the HSB successfully accommodated the
AgNP in the matrix and released them in a controlled way. The release dynamics of
AgNP by HSB was described by UV–vis absorption spectroscopy. The released
nanoparticles were evaluated by Transmission Electron Microscopy (TEM) and Dynamic
Light Scattering (DLS) measurements. In addition, the cytotoxic and genotoxic
effects were evaluated using the Allium cepa assay. The results showed no cytotoxic
effect of HSB-AgNP in all studied concentrations. The genotoxic effect was observed
in HSB-AgNP at the two highest concentrations, however not at the lowest
concentration. Thus, the addition of AgNP at the lowest concentration can improve
the pharmacological activity of HSB without causing a toxic effect on vegetal
cells. Therefore, the H. speciosa latex biomembrane presented in this paper
combines angiogenic, anti-inflammatory and antibacterial properties and can be
considered potentially new biomaterial for wound-healing. © 2018
AU - Almeida, L. M.
AU - Magno, L. N.
AU - Pereira, A. C.
AU - Guidelli, É J.
AU - Filho, O. B.
AU - Kinoshita, A.
AU - Gonçalves, P. J.
DB - Scopus
DO - 10.1016/j.saa.2018.11.050
KW - Allium cepa model
Angiogenic activity
Antibacterial properties
Biomaterial
Biomembrane
Hancornia speciosa latex
Nanoparticles
Apocynaceae
Delayed-Action Preparations
Dynamic Light Scattering
Materials Testing
Membranes, Artificial
Metal Nanoparticles
Microscopy, Electron, Transmission
Mutagenicity Tests
Onions
Silver
Absorption spectroscopy
Biomaterials
Dynamic light scattering
High resolution transmission electron microscopy
Metal nanoparticles
Tissue regeneration
Transmission electron microscopy
metal nanoparticle
silver
Allium cepa
Anti-fungal properties
Anti-inflammatories
Antibacterial and antifungal activity
Bio membranes
Pharmacological activity
VIS absorption spectroscopy
artificial membrane
chemistry
delayed release formulation
dose response
drug effect
materials testing
mutagen testing
onion
pharmacology
procedures
M3 - Article
PY - 2019
SP - 329-334
ST - Toxicity of silver nanoparticles released by Hancornia speciosa (Mangabeira)
biomembrane
T2 - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
TI - Toxicity of silver nanoparticles released by Hancornia speciosa (Mangabeira)
biomembrane
85056860006&doi=10.1016%2fj.saa.2018.11.050&partnerID=40&md5=5eb79921e151684558ba8f
a194045593
VL - 210
ID - 5406
ER -
TY - JOUR
AB - Recent research has shown that latex from different species is able to
produce tissue replacement and regeneration. Particularly, biomembranes obtained
from Hancornia speciosa latex (HSB) have shown high angiogenic and osteogenic
activity. Considering new materials for wound healing, it would be interesting to
develop a product combining antibacterial and antifungal activities. Silver
nanoparticles (AgNP) have been commonly used for this purpose in medicinal products
and devices for decades. In order to combine angiogenic, antibacterial and
antifungal properties on the same platform, we developed an HSB containing 3
concentrations of AgNP. It was observed that the HSB successfully accommodated the
AgNP in the matrix and released them in a controlled way. The release dynamics of
AgNP by HSB was described by UV-vis absorption spectroscopy. The released
nanoparticles were evaluated by Transmission Electron Microscopy (TEM) and Dynamic
Light Scattering (DLS) measurements. In addition, the cytotoxic and genotoxic
effects were evaluated using the Allium cepa assay. The results showed no cytotoxic
effect of HSB-AgNP in all studied concentrations. The genotoxic effect was observed
in HSB-AgNP at the two highest concentrations, however not at the lowest
concentration. Thus, the addition of AgNP at the lowest concentration can improve
the pharmacological activity of HSB without causing a toxic effect on vegetal
cells. Therefore, the H. speciosa latex biomembrane presented in this paper
combines angiogenic, anti-inflammatory and antibacterial properties and can be
considered potentially new biomaterial for wound-healing. (C) 2018 Elsevier B.V.
All rights reserved.
AN - WOS:000456223400042
AU - Almeida, L. M.
AU - Magno, L. N.
AU - Pereira, A. C.
AU - Guidelli, E. J.
AU - Baffa, O.
AU - Kinoshita, A.
AU - Goncalves, P. J.
DA - MAR 5
DO - 10.1016/j.saa.2018.11.050
PY - 2019
SN - 1386-1425
SP - 329-334
ST - Toxicity of silver nanoparticles released by Hancornia speciosa (Mangabeira)
biomembrane
T2 - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
TI - Toxicity of silver nanoparticles released by Hancornia speciosa (Mangabeira)
biomembrane
VL - 210
ID - 6001
ER -
TY - JOUR
AB - Pre-existing conditions modulate sensitivity to numerous xenobiotic exposures
such as air pollution. Specifically, individuals suffering from metabolic syndrome
(MetS) demonstrate enhanced acute inflammatory responses following particulate
matter inhalation. The mechanisms associated with these exacerbated inflammatory
responses are unknown, impairing interventional strategies and our understanding of
susceptible populations. We hypothesize MetS-associated lipid dysregulation
influences mediators of inflammatory resolution signaling contributing to increased
acute pulmonary toxicity. To evaluate this hypothesis, healthy and MetS mouse
models were treated with either 18-hydroxy eicosapentaenoic acid (18-HEPE), 14-
hydroxy docosahexaenoic acid (14-HDHA), 17-hydroxy docosahexaenoic acid (17-HDHA),
or saline (control) via intraperitoneal injection prior to oropharyngeal aspiration
of silver nanoparticles (AgNP). In mice receiving saline treatment, AgNP exposure
resulted in an acute pulmonary inflammatory response that was exacerbated in MetS
mice. A targeted lipid assessment demonstrated 18-HEPE, 14-HDHA, and 17-HDHA
treatments altered lung levels of specialized pro-resolving lipid mediators (SPMs).
14-HDHA and 17-HDHA treatments more efficiently reduced the exacerbated acute
inflammatory response in AgNP exposed MetS mice as compared to 18-HEPE. This
included decreased neutrophilic influx, diminished induction of inflammatory
cytokines/chemokines, and reduced alterations in SPMs. Examination of SPM receptors
determined baseline reductions in MetS mice compared to healthy as well as
decreases due to AgNP exposure. Overall, these results demonstrate AgNP exposure
disrupts inflammatory resolution, specifically 14-HDHA and 17-HDHA derived SPMs, in
MetS contributing to exacerbated acute inflammatory responses. Our findings
identify a potential mechanism responsible for enhanced susceptibility in MetS that
can be targeted for interventional therapeutic approaches. © 2021 Elsevier Inc.
AU - Alqahtani, S.
AU - Xia, L.
AU - Jannasch, A.
AU - Ferreira, C.
AU - Franco, J.
AU - Shannahan, J. H.
C7 - 115730
DB - Scopus
DO - 10.1016/j.taap.2021.115730
KW - Inflammatory resolution
Lipid supplementation
Metabolic syndrome
Nanoparticles
Nanotoxicity
Omega-3 polyunsaturated fatty acids
Specialized pro-resolving mediators
Susceptibility
Animals
Cytokines
Diet, High-Fat
Docosahexaenoic Acids
Hydroxyeicosatetraenoic Acids
Lipid Metabolism
Lung
Male
Metabolic Syndrome
Metal Nanoparticles
Mice, Inbred C57BL
Pneumonia
Signal Transduction
Silver Compounds
14 hydroxy docosahexaenoic acid
17 hydroxy docosahexaenoic acid
18 hydroxy eicosapentaenoic acid
arachidonate 15 lipoxygenase
chemokine
chemokine like receptor 1
CXCL1 chemokine
cytokine
G protein coupled receptor 18
interleukin 1beta
interleukin 6
isoflurane
leucine rich repeat containing G protein coupled receptor 6
macrophage inflammatory protein
receptor
silver nanoparticle
sodium chloride
unclassified drug
unsaturated fatty acid
14-hydroxydocosahexaenoic acid
17-hydroxy-4,7,10,13,15,19-docosahexaenoic acid
18-hydroxy-5,8,11,14-eicosatetraenoic acid
autacoid
docosahexaenoic acid
hydroxyicosatetraenoic acid
metal nanoparticle
silver derivative
animal cell
animal experiment
animal model
animal tissue
Article
bronchoalveolar lavage fluid
controlled study
exposure
gene expression profiling
lipid metabolism
lung injury
lung parenchyma
male
metabolic syndrome X
mouse
neutrophil
nonhuman
pneumonia
protein induction
signal transduction
animal
C57BL mouse
comparative study
complication
disease model
drug effect
genetics
lipid diet
lung
metabolism
M3 - Article
PY - 2021
ST - Disruption of pulmonary resolution mediators contribute to exacerbated silver
nanoparticle-induced acute inflammation in a metabolic syndrome mouse model
T2 - Toxicology and Applied Pharmacology
TI - Disruption of pulmonary resolution mediators contribute to exacerbated silver
nanoparticle-induced acute inflammation in a metabolic syndrome mouse model
85116321846&doi=10.1016%2fj.taap.2021.115730&partnerID=40&md5=9e7dff73b487cd9b7cedc
40ff636d221
VL - 431
ID - 5233
ER -
TY - JOUR
AB - Background: Metabolic syndrome (MetS) exacerbates susceptibility to
inhalation exposures such as particulate air pollution, however, the mechanisms
responsible remain unelucidated. Previously, we determined a MetS mouse model
exhibited exacerbated pulmonary inflammation 24 h following AgNP exposure compared
to a healthy mouse model. This enhanced response corresponded with reduction of
distinct resolution mediators. We hypothesized silver nanoparticle (AgNP) exposure
in MetS results in sustained pulmonary inflammation. Further, we hypothesized
treatment with resolvin D1 (RvD1) will reduce exacerbations in AgNP-induced
inflammation due to MetS. Results: To evaluate these hypotheses, healthy and MetS
mouse models were exposed to vehicle (control) or AgNPs and a day later, treated
with resolvin D1 (RvD1) or vehicle (control) via oropharyngeal aspiration.
Pulmonary lung toxicity was evaluated at 3-, 7-, 14-, and 21-days following AgNP
exposure. MetS mice exposed to AgNPs and receiving vehicle treatment, demonstrated
exacerbated pulmonary inflammatory responses compared to healthy mice. In the AgNP
exposed mice receiving RvD1, pulmonary inflammatory response in MetS was reduced to
levels comparable to healthy mice exposed to AgNPs. This included decreases in
neutrophil influx and inflammatory cytokines, as well as elevated anti-inflammatory
cytokines. Conclusions: Inefficient resolution may contribute to enhancements in
MetS susceptibility to AgNP exposure causing an increased pulmonary inflammatory
response. Treatments utilizing specific resolution mediators may be beneficial to
individuals suffering MetS following inhalation exposures. © 2022, The Author(s).
AU - Alqahtani, S.
AU - Xia, L.
C7 - 54
DB - Scopus
DO - 10.1186/s12989-022-00495-6
IS - 1
KW - Chronic inflammation
Failure of resolution
Inflammatory resolution
Lipid supplementation
Metabolic syndrome (MetS)
Nanoparticles (NPs)
Nanotoxicity
Omega-3 polyunsaturated fatty acids
Resolvin D1 (RvD1)
Specialized pro-resolving mediators (SPMs)
Susceptibility
Animals
Cytokines
Docosahexaenoic Acids
Inflammation
Metabolic Syndrome
Metal Nanoparticles
Mice
Pneumonia
Silver
cytokine
resolvin D1
silver nanoparticle
unclassified drug
metal nanoparticle
silver
animal cell
animal experiment
animal model
animal tissue
Article
controlled study
inflammation
lung toxicity
male
mouse
nanotoxicology
neutrophil chemotaxis
nonhuman
pneumonia
risk factor
animal
disease model
metabolism
M3 - Article
PY - 2022
ST - Enhanced silver nanoparticle-induced pulmonary inflammation in a metabolic
syndrome mouse model and resolvin D1 treatment
T2 - Particle and Fibre Toxicology
TI - Enhanced silver nanoparticle-induced pulmonary inflammation in a metabolic
syndrome mouse model and resolvin D1 treatment
85135590074&doi=10.1186%2fs12989-022-00495-
6&partnerID=40&md5=9146ab791a0b9762ed4cd30612e9a875
VL - 19
ID - 4968
ER -
TY - JOUR
AB - Background: Silver nanoparticles (AgNPs) are attractive substrates for new
medicinal treatments. Biochar is pyrolyzed biomass. Its porous architecture allows
it to hold and gather minuscule particles, through which nanoparticles can
accumulate in its porous structure. This study examined AgNPs’ antibacterial and
anticancer properties alone and combined with biochar. Methods: The fungus
Emericella dentata was responsible for biosynthesis of AgNPs. The characterization
of AgNPs using STEM images and a Zetasizer was carried out. Accordingly, the
antibacterial and antiproliferation activity of AgNPs and biochar was studied using
MIC and MTT assays, respectively. To evaluate the antiangiogenic and anti-
inflammatory effects of AgNPs with biochar, VEGF and cytokines including TNF alpha,
IL-6 and IL-beta were tested using an ELISA assay. Results: The size of the AgNPs
ranged from 10 to 80 nm, with more than 70% of them being smaller than 40 nm. The
combination of AgNPs and biochar enhanced the antibacterial activity against all
tested bacteria. Furthermore, this combination showed antiproliferative properties
against HT29 cancer cells with high selectivity to fibroblasts at low
concentrations. AgNPs with biochar significantly reduced VEGF and proinflammatory
cytokine expression levels. Conclusions: Biochar and AgNPs may be novel treatments
for bacteria and colorectal cancer cells, according to the current findings. © 2023
by the authors.
AU - Alqaraleh, M.
AU - Khleifat, K. M.
AU - Abu Hajleh, M. N.
AU - Farah, H. S.
AU - Ahmed, K. A. A.
C7 - 597
DB - Scopus
DO - 10.3390/antibiotics12030597
IS - 3
KW - antibacterial
antitumor activity
biochar
colorectal cancer
charcoal
fungal extract
graphene oxide
interleukin 1beta
interleukin 6
protein p53
silver nanoparticle
vasculotropin
animal cell
antifungal activity
apoptosis
Article
bacterial growth
bacterium isolation
broth dilution
cell proliferation
cell viability
circular dichroism
colorectal cancer cell line
cytotoxicity assay
disk diffusion
DNA damage
DNA fragmentation
fibroblast
gene expression
HT-29 cell line
human
human cell
hydrophobicity
IC50
MTT assay
nonhuman
nucleotide sequence
oxidative stress
protein expression
ultraviolet spectroscopy
zeta potential
M3 - Article
PY - 2023
ST - Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal
Cancer Cells and Pathogenic Bacteria
T2 - Antibiotics
TI - Fungal-Mediated Silver Nanoparticle and Biochar Synergy against Colorectal
Cancer Cells and Pathogenic Bacteria
85151741470&doi=10.3390%2fantibiotics12030597&partnerID=40&md5=29d87a14b5061f037e5d
d7270cf27101
VL - 12
ID - 5030
ER -
TY - CHAP
AB - Mast cells are key effector cells in inflammatory and allergic immune
responses such as asthma, rhinitis, and atopic dermatitis. Activation of mast cells
leads to immediate release of preformed mediators such as histamine and proteases,
which can regulate vascular permeability and the function of a number of immune and
nonimmune cells. Engineered nanomaterials (ENM) have been utilized for a wide range
of applications and introduced into a number of consumer products; yet the
consequent increase in human exposure and any potential adverse effects have not
been fully evaluated. Modulation of the immune system function has been shown to be
a major toxicological consequence of ENM exposure. The implication of mast cells in
ENM-mediated toxicity, including the most widely utilized carbon and metal-based
ENMs, has been previously demonstrated; and therefore, understanding direct ENM
interaction with mast cells at the cellular and molecular level is of critical
importance for the safe implementation of ENMs into consumer products. © Springer
Science+Business Media, LLC, part of Springer Nature 2019.
AU - Alsaleh, N. B.
AU - Brown, J. M.
DB - Scopus
DO - 10.1007/978-1-4939-8916-4_2
KW - Degranulation
Engineered nanomaterials
Immunotoxicity
Mast cell
Nanoparticles
Nanotoxicity
Animals
Carbon
Cell Degranulation
Cell Survival
Cells, Cultured
Consumer Product Safety
Humans
Hypersensitivity
Mast Cells
Metals
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Models, Animal
Nanostructures
Primary Cell Culture
Toxicity Tests
beta n acetylhexosaminidase
carbon
chemokine
cytokine
Fc receptor
histamine
lysosome associated membrane protein 2
proteinase
silver nanoparticle
stem cell factor receptor
metal
nanomaterial
animal cell
animal experiment
animal model
cell maturation
cell viability
controlled study
cytokine release
human
immune system
immunocompetent cell
mast cell
mast cell degranulation
mouse
nanotoxicology
nonhuman
passive skin anaphylaxis
protein expression
animal
Bagg albino mouse
C57BL mouse
cell culture
cell survival
degranulation
devices
hypersensitivity
immunology
primary cell culture
procedures
product safety
toxicity testing
PY - 2019
SP - 31-45
ST - Methods for assessing mast cell responses to engineered nanomaterial exposure
T2 - Methods in Molecular Biology
TI - Methods for assessing mast cell responses to engineered nanomaterial exposure
85058732936&doi=10.1007%2f978-1-4939-8916-
4_2&partnerID=40&md5=d56c02295a069d59d07a6b132062d392
VL - 1894
ID - 5426
ER -
TY - JOUR
AB - Engineered nanomaterials (ENM) are being used in a wide range of consumer
products and pharmaceuticals; hence, there is an increasing risk for human exposure
and potential adverse outcomes. The immune system, vital in host defense and
protection against environmental agents, is typically initiated and executed by
innate effector immune cells including macrophages and neutrophils. Previous
literature has reported the immune system as a major target of ENM toxicity;
however, there is inconsistency regarding the immunotoxicity of ENM. This could be
attributed to differences in ENM physicochemical properties, cellular models
examined, biocorona formation, etc. Thus, the current study examined the toxicity
and immunomodulatory effects of silver nanoparticles (AgNP), one of the most
utilized ENM in consumer and medical products, in two key innate immune cell
models, e.g. RAW 264.7 cells (macrophages) and differentiated MPRO 2.1 cells
(promyelocytes/neutrophils). The results showed that despite a generation of
reactive oxygen species, exposure to 20 nm citrate-coated AgNP was not associated
with major oxidative damage, inflammatory responses, nor cytotoxicity.
Nevertheless, and most importantly, pre-exposure to the AgNP for 24 h enhanced RAW
264.7 cell phagocytic ability as well as the release of inflammatory cytokine
interleukin-6 in response to lipopolysaccharide (LPS). In MPRO 2.1 cells, AgNP pre-
exposure also resulted in enhanced phagocytic ability; however, these cells
manifest reduced cell degranulation (elastase release) and oxidative burst in
response to phorbol myristate acetate (PMA). Taken together, these findings
indicated to us that exposure to AgNP, despite not being directly (cyto)toxic to
these cells, had the potential to alter immune cell responses. The findings
underscore the import of assessing immune cell function post-exposure to ENM beyond
the standard endpoints such as oxidative stress and cytotoxicity. In addition,
these findings further illustrate the importance of understanding the underlying
molecular mechanisms of ENM-cellular interactions, particularly in the immune
system. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as
Taylor & Francis Group.
AU - Alsaleh, N. B.
AU - Minarchick, V. C.
AU - Mendoza, R. P.
AU - Sharma, B.
AU - Podila, R.
AU - Brown, J. M.
DB - Scopus
DO - 10.1080/1547691X.2019.1588928
IS - 1
KW - immune activation
immunomodulation
immunotoxicity
nanotoxicity
Nanotoxicology
suppression
Animals
Cell Degranulation
Granulocyte Precursor Cells
Interleukin-6
Lipopolysaccharides
Metal Nanoparticles
Mice
Neutrophils
Oxidative Stress
Particle Size
Phagocytosis
RAW 264.7 Cells
Silver
Toxicity Tests
interleukin 6
lipopolysaccharide
silver nanoparticle
interleukin-6, mouse
metal nanoparticle
silver
animal cell
Article
cell function
cell viability
controlled study
drug uptake
neutrophil
nonhuman
phagocytosis
priority journal
RAW 264.7 cell line
animal
degranulation
drug effect
granulocyte precursor
immunology
metabolism
mouse
oxidative stress
particle size
toxicity testing
M3 - Article
PY - 2019
SP - 63-73
ST - Silver nanoparticle immunomodulatory potential in absence of direct
cytotoxicity in RAW 264.7 macrophages and MPRO 2.1 neutrophils
T2 - Journal of Immunotoxicology
TI - Silver nanoparticle immunomodulatory potential in absence of direct
cytotoxicity in RAW 264.7 macrophages and MPRO 2.1 neutrophils
85069285768&doi=10.1080%2f1547691X.2019.1588928&partnerID=40&md5=46bda8a6cc6b06f00e
5ed11b4a35eabd
VL - 16
ID - 5434
ER -
TY - JOUR
AB - Three biodegradable wound dressing based on binary Collagen (COL), Hyaluronic
acid (HA) crosslinked loaded with silver nanoparticles (AgNPs), Gentamicin (GENT)
and AgNPs/GENT successfully prepared using freeze drying technique. Chemical
evaluations for synthesized membranes were carried out using FTIR- ATR. While
physical properties were evaluated through swelling and degradation percent.
Antibacterial activity was evaluated against G+, G-, yeast and fungi. Finally,
cytotoxicity and wound healing evaluations were carried out against skin fibroblast
normal cell line, while anti-inflammatory evaluated using RAW 264.7 macrophage cell
line. The three produced membrane showed physically interaction between polymer
network and the loaded antibiotic. Swelling properties showed superior results for
three membranes. Degradability of prepared sheets was rapidly no more than three
days. Toxicity evaluations and anti-inflammatory showed superior results for all
examined samples except mixed with AgNPs and Gentamicin (GENT). Antibacterial
activity showed resistance to G+, G- and yeast. All prepared sheet showed safe
towards cell except COL/HA/AgNPs/GENT. Wound healing studied showed efficient of
both COL/HA/AgNPs and COL/HA/GENT compared to blank and mixed membrane
COL/HA/AgNPs/GENT. The obtained results recommended COL/HA loaded individually
either AgNPs or Gentamicin (GENT) as antibacterial and wound healing sheet rather
than mixed prepared membrane. © 2023 Elsevier B.V.
AU - AlSalem, H. S.
AU - Bukhari, A. A. H.
C7 - 124700
DB - Scopus
DO - 10.1016/j.ijbiomac.2023.124700
KW - Collagen
Gentamicin
Hyaluronic acid
Wound healing
Bandages
Gentamicins
Hyaluronic Acid
Metal Nanoparticles
Saccharomyces cerevisiae
Silver
Wound Healing
Cell culture
Crosslinking
Membranes
Organic acids
Yeast
antibiotic agent
antiinfective agent
collagen
gentamicin
hyaluronic acid
nitric oxide
silver nanoparticle
metal nanoparticle
silver
Anti-inflammatories
Chemical evaluation
Crosslinked
Drying technique
Freeze drying
Wound dressings
Wound healing applications
Article
attenuated total reflectance Fourier transform infrared spectroscopy
biodegradability
clinical article
cytotoxicity
fibroblast
freeze drying
human
human cell
macrophage cell line
particle size
RAW 264.7 cell line
skin fibroblast
swelling
wound healing
wound healing assay
chemistry
metabolism
M3 - Article
PY - 2023
ST - Biodegradable wound dressing-based collagen/hyaluronic acid loaded
antibacterial agents for wound healing application
TI - Biodegradable wound dressing-based collagen/hyaluronic acid loaded
antibacterial agents for wound healing application
85159578926&doi=10.1016%2fj.ijbiomac.2023.124700&partnerID=40&md5=c235efe2976d37c58
5739a6ea21335fd
VL - 242
ID - 5010
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have recently gained interest in the medical
field because of their biological features. The present study aimed at screening
Rhizophora apiculata secondary metabolites, quantifying their flavonoids and total
phenolics content, green synthesis and characterization of R. apiculata silver
nanoparticles. In addition, an assessment of in vitro cytotoxic, antioxidant, anti-
inflammatory and wound healing activity of R. apiculata and its synthesized AgNPs
was carried out. The powdered plant material (leaves) was subjected to Soxhlet
extraction to obtain R. apiculata aqueous extract. The R. apiculata extract was
used as a reducing agent in synthesizing AgNPs from silver nitrate. The synthesized
AgNPs were characterized by UV-Vis, SEM-EDX, XRD, FTIR, particle size analyzer and
zeta potential. Further aqueous leaf extract of R. apiculata and AgNPs was
subjected for in vitro antioxidant, anti-inflammatory, wound healing and cytotoxic
activity against A375 (Skin cancer), A549 (Lung cancer), and KB-3-1 (Oral cancer)
cell lines. All experiments were repeated three times (n = 3), and the results were
given as the mean +/- SEM. The flavonoids and total phenolics content in R.
apiculata extract were 44.18 +/- 0.086 mg/g of quercetin and 53.24 +/- 0.028 mg/g
of gallic acid, respectively. SEM analysis revealed R. apiculata AgNPs with
diameters ranging from 35 to 100 nm. XRD confirmed that the synthesized silver
nanoparticles were crystalline in nature. The cytotoxicity cell viability assay
revealed that the AgNPs were less toxic (IC50 105.5 mu g/mL) compared to the R.
apiculata extract (IC50 47.47 mu g/mL) against the non-cancerous fibroblast L929
cell line. Antioxidant, anti-inflammatory, and cytotoxicity tests revealed that
AgNPs had significantly more activity than the plant extract. The AgNPs inhibited
protein denaturation by a mean percentage of 71.65%, which was equivalent to the
standard anti-inflammatory medication diclofenac (94.24%). The AgNPs showed
considerable cytotoxic effect, and the percentage of cell viability against skin
cancer, lung cancer, and oral cancer cell lines was 31.84%, 56.09% and 22.59%,
respectively. R. apiculata AgNPs demonstrated stronger cell migration and
percentage of wound closure (82.79%) compared to the plant extract (75.23%). The
overall results revealed that R. apiculata AgNPs exhibited potential antioxidant,
anti-inflammatory, wound healing, and cytotoxic properties. In future, R. apiculata
should be further explored to unmask its therapeutic potential and the mechanistic
pathways of AgNPs should be studied in detail in in vivo animal models.
AN - WOS:000868134100001
AU - Alsareii, S. A.
AU - Alamri, A. M.
AU - AlAsmari, M. Y.
AU - Bawahab, M. A.
AU - Mahnashi, M. H.
AU - Shaikh, I. A.
AU - Shettar, A. K.
AU - Hoskeri, J. H.
AU - Kumbar, V.
C7 - 6306
DA - OCT
DO - 10.3390/molecules27196306
IS - 19
PY - 2022
SN - 1420-3049
ST - Synthesis and Characterization of Silver Nanoparticles from Rhizophora
apiculata and Studies on Their Wound Healing, Antioxidant, Anti-Inflammatory, and
Cytotoxic Activity
T2 - MOLECULES
TI - Synthesis and Characterization of Silver Nanoparticles from Rhizophora
Cytotoxic Activity
VL - 27
ID - 5843
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have recently gained interest in the medical
field because of their biological features. The present study aimed at screening
Rhizophora apiculata secondary metabolites, quantifying their flavonoids and total
phenolics content, green synthesis and characterization of R. apiculata silver
nanoparticles. In addition, an assessment of in vitro cytotoxic, antioxidant, anti-
inflammatory and wound healing activity of R. apiculata and its synthesized AgNPs
was carried out. The powdered plant material (leaves) was subjected to Soxhlet
extraction to obtain R. apiculata aqueous extract. The R. apiculata extract was
used as a reducing agent in synthesizing AgNPs from silver nitrate. The synthesized
AgNPs were characterized by UV-Vis, SEM-EDX, XRD, FTIR, particle size analyzer and
zeta potential. Further aqueous leaf extract of R. apiculata and AgNPs was
subjected for in vitro antioxidant, anti-inflammatory, wound healing and cytotoxic
activity against A375 (Skin cancer), A549 (Lung cancer), and KB-3-1 (Oral cancer)
cell lines. All experiments were repeated three times (n = 3), and the results were
given as the mean ± SEM. The flavonoids and total phenolics content in R. apiculata
extract were 44.18 ± 0.086 mg/g of quercetin and 53.24 ± 0.028 mg/g of gallic acid,
respectively. SEM analysis revealed R. apiculata AgNPs with diameters ranging from
35 to 100 nm. XRD confirmed that the synthesized silver nanoparticles were
crystalline in nature. The cytotoxicity cell viability assay revealed that the
AgNPs were less toxic (IC50 105.5 µg/mL) compared to the R. apiculata extract (IC50
47.47 µg/mL) against the non-cancerous fibroblast L929 cell line. Antioxidant,
anti-inflammatory, and cytotoxicity tests revealed that AgNPs had significantly
more activity than the plant extract. The AgNPs inhibited protein denaturation by a
mean percentage of 71.65%, which was equivalent to the standard anti-inflammatory
medication diclofenac (94.24%). The AgNPs showed considerable cytotoxic effect, and
the percentage of cell viability against skin cancer, lung cancer, and oral cancer
cell lines was 31.84%, 56.09% and 22.59%, respectively. R. apiculata AgNPs
demonstrated stronger cell migration and percentage of wound closure (82.79%)
compared to the plant extract (75.23%). The overall results revealed that R.
apiculata AgNPs exhibited potential antioxidant, anti-inflammatory, wound healing,
and cytotoxic properties. In future, R. apiculata should be further explored to
unmask its therapeutic potential and the mechanistic pathways of AgNPs should be
studied in detail in in vivo animal models. © 2022 by the authors.
AU - Alsareii, S. A.
AU - Manaa Alamri, A.
AU - AlAsmari, M. Y.
AU - Bawahab, M. A.
AU - Mahnashi, M. H.
AU - Shaikh, I. A.
AU - Shettar, A. K.
AU - Hoskeri, J. H.
AU - Kumbar, V.
C7 - 6306
DB - Scopus
DO - 10.3390/molecules27196306
IS - 19
KW - anti-inflammatory
antioxidant
aqueous extract
cytotoxicity
in vitro
Rhizophora apiculata leaf
wound healing
Animals
Antioxidants
Diclofenac
Gallic Acid
Metal Nanoparticles
Mouth Neoplasms
Plant Extracts
Quercetin
Reducing Agents
Rhizophoraceae
Silver
Silver Nitrate
Wound Healing
antiinfective agent
diclofenac
gallic acid
metal nanoparticle
plant extract
quercetin
reducing agent
silver
silver nitrate
animal
chemistry
mouth tumor
M3 - Article
PY - 2022
ST - Synthesis and Characterization of Silver Nanoparticles from Rhizophora
Cytotoxic Activity
T2 - Molecules
TI - Synthesis and Characterization of Silver Nanoparticles from Rhizophora
Cytotoxic Activity
85139873443&doi=10.3390%2fmolecules27196306&partnerID=40&md5=0e2297dc472bfb8b49a758
3e84d72a0d
VL - 27
ID - 5146
ER -
TY - JOUR
AB - Six new Ag(I) coordination complexes, namely [Ag4(μ-dicl)4(2-pic)3] 1,
[Ag2(μ-mef)2(2-pic)2] 2, [Ag2(μ-dicl)2(3-pic)2] 3, [Ag2(μ-mef)2(3-pic)2] 4, [Ag2(μ-
dicl)2(4-pic)2] 5 and [Ag2(μ-mef)2(4-pic)2] 6, were synthesized and characterized
by elemental analysis, FT-IR and thermal analysis techniques. The crystal
structures of 1, 3, 4 and 5 were determined by X-ray diffraction analysis, whereas
2 and 6 were obtained as microcrystalline powders. X-ray diffraction analysis
demonstrated that 1 occurs as a tetranuclear complex, while 3, 4 and 5 have
binuclear structures. A short Ag⋯Ag distances of 2.8446 and 2.8823 Å for 1, 2.8409
Å for 3, 2.8942 Å for 4 and 2.8423 Å for 5 were found in the complexes, indicating
that argentophilic interactions exist between the silver ions. In 1, the dicl
ligands act as μ3-O,O′,O′ bridging ligands between three Ag(I) ions. In 3, 4 and 5,
the dicl and mef ligands behave as bridging ligands and bind two Ag(I) ions
together with the carboxylato oxygen atoms. The simultaneous TG/DTG and DTA
techniques were applied to interpret the mass losses, decomposition temperatures
and corresponding processes of the thermal behaviours of the complexes. The
experimental and calculated mass losses of the proposed structures for complexes 2
and 6 are quite compatible with each other and these complexes have very similar
FT-IR spectra to the other complexes, which reflect their same structural
geometries and characteristics. The cytotoxic activities of the complexes (1–6)
were tested against three different cancer cell lines, MCF-7, HT-29 and HepG2, and
one normal cell line, 3 T3-L1. XTT assay results demonstrated that although all the
silver(I) complexes showed good cytotoxic activity, depending on the cell types
tested, the complexes with mefanamic acid (2, 4 and 6) were found to predominate
over those with diclofenac (1, 3 and 5), as well as the superiority of 3-picoline
to 4-picoline and 2-picoline. Moreover, compared with the cytotoxic potential of
carboplatin, 4 especially exhibited a significant cancer cell inhibitory rate and
lower cytotoxicity toward the normal cell line, with much higher selectivity
indexes. © 2018 Elsevier Ltd
AU - Altay, A.
AU - Caglar, S.
AU - Caglar, B.
AU - Sahin, O.
DB - Scopus
DO - 10.1016/j.poly.2018.05.038
KW - Cell culture
Cytotoxicity
Diclofenac
Mefenamic acid
Silver(I) complexes
M3 - Article
PY - 2018
SP - 160-170
ST - Synthesis, structural, thermal elucidation and in vitro anticancer activity
of novel silver(I) complexes with non-steroidal anti-inflammatory drugs diclofenac
and mefenamic acid including picoline derivatives
T2 - Polyhedron
TI - Synthesis, structural, thermal elucidation and in vitro anticancer activity
of novel silver(I) complexes with non-steroidal anti-inflammatory drugs diclofenac
and mefenamic acid including picoline derivatives
85047825401&doi=10.1016%2fj.poly.2018.05.038&partnerID=40&md5=56aa82bea48332fceb009
bebacfa4e2e
VL - 151
ID - 5402
ER -
TY - JOUR
AB - Currently, nanotechnology is perceived as a promising science that produces
materials with diverse unique properties at a nanometric scale. Biocompatibility
tests of poly-epsilon-caprolactone nanofibers, embedded with silver nanoparticles
manufactured by means of the electrospinning technique, were carried out in Wistar
rats to be used as oral dressings for the eradication of bacteria. Solutions of
12.5, 25, 50 and 100 mM of silver nitrate were made using N-dimethylformamide (DMF)
and tetrahydrofuran (THF) as reducing solvents with 8% of poly-epsilon-caprolactone
(PCL) polymer. The solutions were electrospun, and the nanofibers obtained in the
process were characterized by infrared spectroscopy, Raman spectroscopy, dark field
optical microscopy, scanning electron microscopy and X-ray scattering spectroscopy.
The nanofibers had an average diameter of 400 +/- 100 nm. Once the characterization
of the material was done, three implants of each concentration of the nanofibers
were formed and placed in the subcutaneous tissue of the rats. Three experimental
subjects were used, leaving the material in them for a length of two, four and six
weeks, respectively. The rats showed good healing, with the lesions completely
healed at four weeks after implantation. After that time, biopsies were taken, and
histopathological sections were made to evaluate the inflammatory infiltrate. The
tissues of the rats presented chronic inflammatory infiltrate composed mainly of
lymphocytes and giant multinucleated cells. The material was rejected by the rats
when a layer of collagen and fibroblasts was produced, coating the material, a
process characteristic of a foreign body reaction.
AN - WOS:000638784800001
AU - Alvarez-Ortega, O.
AU - Ruiz-Ramirez, L. R.
AU - Garibay-Alvarado, J. A.
AU - Donohue-Cornejo, A.
AU - Espinosa-Cristobal, L. F.
AU - Cuevas-Gonzalez, J. C.
AU - Reyes-Lopez, S. Y.
C7 - 1135
DA - APR
DO - 10.3390/polym13071135
IS - 7
PY - 2021
SN - 2073-4360
ST - Preliminary Biocompatibility Tests of Poly-epsilon-Caprolactone/Silver
Nanofibers in Wistar Rats
T2 - POLYMERS
TI - Preliminary Biocompatibility Tests of Poly-epsilon-Caprolactone/Silver
Nanofibers in Wistar Rats
VL - 13
ID - 6098
ER -
TY - JOUR
AB - Currently, nanotechnology is perceived as a promising science that produces
materials with diverse unique properties at a nanometric scale. Biocompatibility
tests of poly-ε-caprolactone nanofibers, embedded with silver nanoparticles
manufactured by means of the electrospinning technique, were carried out in Wistar
rats to be used as oral dressings for the eradication of bacteria. Solutions of
12.5, 25, 50 and 100 mM of silver nitrate were made using N-dimethylformamide (DMF)
and tetrahydrofuran (THF) as reducing solvents with 8% of poly-ε-caprolactone (PCL)
polymer. The solutions were electrospun, and the nanofibers obtained in the process
were characterized by infrared spectroscopy, Raman spectroscopy, dark field optical
microscopy, scanning electron microscopy and X-ray scattering spectroscopy. The
nanofibers had an average diameter of 400 ± 100 nm. Once the characterization of
the material was done, three implants of each concentration of the nanofibers were
formed and placed in the subcutaneous tissue of the rats. Three experimental
subjects were used, leaving the material in them for a length of two, four and six
weeks, respectively. The rats showed good healing, with the lesions completely
healed at four weeks after implantation. After that time, biopsies were taken, and
histopathological sections were made to evaluate the inflammatory infiltrate. The
tissues of the rats presented chronic inflammatory infiltrate composed mainly of
lymphocytes and giant multinucleated cells. The material was rejected by the rats
when a layer of collagen and fibroblasts was produced, coating the material, a
process characteristic of a foreign body reaction. © 2021 by the authors.
AU - Álvarez-Ortega, O.
AU - Ruiz-Ramírez, L. R.
AU - Garibay-Alvarado, J. A.
AU - Espinosa-Cristóbal, L. F.
AU - Cuevas-González, J. C.
AU - Reyes-López, S. Y.
C7 - 1135
DB - Scopus
DO - 10.3390/polym13071135
IS - 7
KW - Biocompatibility
Foreign body reaction
Inflammation
PCL/Ag
Cell culture
Dimethylformamide
Infrared spectroscopy
Nanofibers
Organic solvents
Scanning electron microscopy
Silver compounds
Tissue
X ray scattering
Biocompatibility tests
Chronic inflammatory
Electrospinning techniques
Experimental subjects
Foreign body reactions
Multinucleated cells
Process characteristics
X-ray scattering spectroscopy
Rats
M3 - Article
PY - 2021
ST - Preliminary biocompatibility tests of poly-ε-caprolactone/silver nanofibers
in wistar rats
T2 - Polymers
TI - Preliminary biocompatibility tests of poly-ε-caprolactone/silver nanofibers
in wistar rats
85104095920&doi=10.3390%2fpolym13071135&partnerID=40&md5=dca91b2315297ead8c427f2144
198b57
VL - 13
ID - 5160
ER -
TY - JOUR
AB - The treatment of wounds is expensive and challenging. Most of the available
wound dressings are not effective and suffer from limitations such as poor
antimicrobial activity, toxicity, inability to provide suitable moisture to the
wound and poor mechanical performance. The use of inappropriate wound dressings can
result in a delayed wound healing process. Nanosize range scaffolds have triggered
great attention because of their attractive properties, which include their
capability to deliver bioactive agents, high surface area, improved mechanical
properties, mimic the extracellular matrix (ECM), and high porosity. Nanofibrous
materials can be further encap-sulated/loaded with metal-based nanoparticles to
enhance their therapeutic outcomes in wound healing applications. The widely
studied metal-based nanoparticles, silver nanoparticles exhibit good properties
such as outstanding antibacterial activity, display antioxidant, and anti-
inflammatory properties, support cell growth, making it an essential bioactive
agent in wound dressings. This review article reports the biological (in vivo and
in vitro) and mechanical outcomes of nanofibrous scaffolds loaded with silver
nanoparticles on wound healing. © 2021 by the authors. Licensee MDPI, Basel,
Switzerland.
AU - Alven, S.
AU - Buyana, B.
AU - Feketshane, Z.
AU - Aderibigbe, B. A.
C7 - 964
DB - Scopus
DO - 10.3390/pharmaceutics13070964
IS - 7
KW - Nanofibers
Nanofibrous mats
Nanofibrous membranes
Nanoparticles
Scaffolds
Wound dressings
Wound treatment
cellulose acetate
chitosan
electrospun nanofiber
gum arabic
macrogol
molecular scaffold
nanofiber
nanomaterial
polycaprolactone
polyethylene
polyglactin
polylactic acid
polyvinyl alcohol
silver nanoparticle
unclassified drug
cell growth
electrospinning
extracellular matrix
human
in vitro study
mechanical stimulation
nanoencapsulation
nonhuman
porosity
Review
surface area
therapy delay
toxicity testing
treatment outcome
wound care
wound healing
M3 - Review
PY - 2021
ST - Electrospun nanofibers/nanofibrous scaffolds loaded with silver nanoparticles
as effective antibacterial wound dressing materials
T2 - Pharmaceutics
TI - Electrospun nanofibers/nanofibrous scaffolds loaded with silver nanoparticles
as effective antibacterial wound dressing materials
85109390469&doi=10.3390%2fpharmaceutics13070964&partnerID=40&md5=2eb7af684bbeb9c9cc
45fbf30a17061f
VL - 13
ID - 5230
ER -
TY - JOUR
AB - The administration of several chemotherapeutic regimens could be conditioned
by the onset of mucositis. The characteristic lesions of the mucositis affect whole
buccal mucosa, That derives from rapid turnover of the oropharyngeal epithelial
surfaces. The mucosa can suffer from direct damage of antiblastic drugs or be
susceptible of microbic infections. Moreover, other factors correlated to the
patients as age, nutritional status, tumor type, oral hygiene and neutrophil count.
Up to date, there is not a standard therapy for the cure or mucositis prevention.
Some formalities can be employed in order to reduce chemo-induced damage: 1)
altering the distribution and the excretion of drugs on the mucosa; 2) stimulating
the basal cells of the mucosa; 3) trying to modify the infectious or inflammatory
risks. The effective oral care, dietary changes and the use of protective topical
and the careful use of topical and systemic anesthetic drugs are the cornerstones
of mucositis care.
AU - Amadio, P.
AU - Ferraù, F.
AU - Priolo, D.
AU - Toscano, G.
AU - Colina, P.
AU - Mare, M.
AU - Zavettieri, M.
AU - La Torre, F.
AU - Mesiti, M.
AU - Maisano, R.
DB - Scopus
IS - 2
KW - Chemotherapy
Mucositis
Prevention
Treatment
Humans
Infection
Mouth Mucosa
Risk Factors
Stomatitis
allopurinol
alpha tocopherol
amphotericin B
anesthetic agent
antiinfective agent
beta carotene
capsaicin
chamomile
corticosteroid
cytokine
doxorubicin
etoposide
fluorouracil
folinic acid
glutamine
granulocyte colony stimulating factor
granulocyte macrophage colony stimulating factor
mafenide
methotrexate
nonsteroid antiinflammatory agent
pilocarpine
placebo
polymyxin B
propantheline bromide
prostaglandin E2
silver nitrate
sucralfate
tobramycin
unindexed drug
cancer
cancer chemotherapy
cancer prevention
clinical trial
controlled clinical trial
controlled study
crossover procedure
cryotherapy
double blind procedure
gastrointestinal toxicity
human
inflammation
mouth hygiene
mouth mucosa
mucosa inflammation
multicenter study
nutritional status
randomized controlled trial
review
ulcer
M3 - Review
PY - 2002
SP - 127-134
ST - Prevenzione e trattamento della mucosite da chemioterapici antiblastici
T2 - Clinica Terapeutica
TI - Prevention and treatment of chemotherapy induced mucositis
0036297911&partnerID=40&md5=f7d97b37ebc614d2dee16145adacc891
VL - 153
ID - 5783
ER -
TY - JOUR
AB - Silver has been used in medical application for its antibacterial,
antifungal, and anti-inflammatory effects. Silver nanoparticles (AgNPs) are
currently in the spotlight. It was shown that their application can be useful in
the management of wounds. Our study was conducted to determine whether AgNPs
(average size 10.43 +/- 4.74 nm) and ionic silver (Ag-I) could affect the wound
healing in the in vitro model of normal human dermal fibroblasts (NHDF). We
evaluated their effect on reactive oxygen species (ROS) generation and the
expression of key transcription factors that coordinate the cellular response to
oxidative stress [nuclear factor (erythroid-derived 2)-like 2 (Nrf2)] and
inflammation [nuclear factor-kappa B (NF-kappa B)], expression of heme oxygenase-1
(HO-1), and interleukin-6 (IL-6) level. Isolated primary NHDF were scratched,
heated (1 h; 42 degrees C), and cultured with AgNPs (0.25, 2.5, and 25 mu g/ml) and
Ag-I (0.025, 0.1, and 0.25 mu g/ml) for 8 or 24 h. The ROS generation, Nrf2, NF-
kappa B, and HO-1 protein expression and IL-6 protein level were then evaluated by
standard methods. Non-cytotoxic concentrations of AgNPs (0.25 and 2.5 mu g/ml) did
not affect the ROS generation but activated the Nrf2/ HO-1 pathway and decreased
the NF-.B expression and IL-6 level in the in vitro wound healing model. AgNPs at
concentrations of 0.25 and 2.5 mu g/ml seem to be suitable for the intended
application as a topical agent for wound healing, although the gene silencing
technique, chemical inhibitors, and detailed time-and concentration-dependent
experiments are needed for a comprehensive study of signaling pathway regulation.
Further investigation is also necessary to exclude any possible adverse effects.
AN - WOS:000411836100001
AU - Ambrozova, N.
AU - Zalesak, B.
AU - Ulrichova, J.
AU - Cizkova, K.
AU - Galandakova, A.
C7 - 108
DA - MAR 13
DO - 10.1007/s11051-017-3809-7
IS - 3
PY - 2017
SN - 1388-0764
1572-896X
ST - Low concentrations of silver nanoparticles have a beneficial effect on wound
healing in vitro
T2 - JOURNAL OF NANOPARTICLE RESEARCH
TI - Low concentrations of silver nanoparticles have a beneficial effect on wound
healing in vitro
VL - 19
ID - 6023
ER -
TY - JOUR
AB - Dysregulation of brain iron homeostasis is central to early neuropathological
events in Alzheimer's disease (AD), including oxidative stress, inflammatory
processes, amyloid deposition, tau phosphorylation, and neuronal cell cycle
regulatory failure, leading to apoptosis. Also, there is a direct link between iron
metabolism and AD pathogenesis, demonstrated by the presence of an iron-responsive
element in the 5′ UTR of the amyloid precursor protein transcript. As a consequence
of these findings, a new paradigm is emerging that includes the development of
iron-chelating neuroprotective-neurorescue drugs with multimodal functions, acting
at various pathological brain targets. This concept is challenging the widely held
assumption that "silver bullet" agents are superior to "dirty drugs" in drug
therapy for neurodegenerative diseases. At best, the so-called magic bullets
exhibit moderate symptomatic activity without modifying the course of disease
progression. The present review elaborates on conventional and novel therapeutic
targets of various multifunctional iron-chelating drugs (e.g., chemically designed
compounds; natural polyphenols) that address multiple central nervous system
etiologies in AD, aimed at preventing or slowing disease evolution. A similar
approach in drug design is being investigated for treatment of cancer, AIDS,
cardiovascular diseases, and depression. © FASEB.
AU - Amit, T.
AU - Avramovich-Tirosh, Y.
AU - Youdim, M. B. H.
AU - Mandel, S.
DB - Scopus
DO - 10.1096/fj.07-8627rev
IS - 5
KW - APP mRNA
Cell cycle
Multifunctional drugs
Neurodegenerative diseases
Alzheimer Disease
Amyloid beta-Protein Precursor
Animals
Brain
Catechin
Cell Cycle
Humans
Hydroxyquinolines
Iron
Iron Chelating Agents
Neurodegenerative Diseases
Neuroprotective Agents
PC12 Cells
Piperazines
Rats
2 (2 amino 3 methoxyphenyl)chromone
5 [4 propargylpiperazin 1 ylmethyl] 8 hydroxyquinoline
5 [n methyl n propargylaminomethyl] 8 hydroxyquinoline
8 quinolinol derivative
alpha tocopherol
antioxidant
chelating agent
clioquinol
deferoxamine
dimercaprol
dipeptidyl carboxypeptidase inhibitor
donepezil
epigallocatechin gallate
feralax
flavonoid
flavopiridol
glucocorticoid
hydroxymethylglutaryl coenzyme A reductase inhibitor
indirubin
iron chelating agent
metal chelate
mimosine
mitogen activated protein kinase inhibitor
nordihydroguaiaretic acid
peroxisome proliferator activated receptor gamma agonist
rasagiline
retinoic acid
rosmarinic acid
tetrathiomolybdic acid
allopathy
Alzheimer disease
cell cycle
cell cycle arrest
cell cycle progression
cell death
cell proliferation
central nervous system
disease course
drug design
drug targeting
human
iron binding capacity
nerve cell
neuroprotection
nonhuman
pathogenesis
priority journal
protein targeting
review
signal transduction
symptom
M3 - Review
PY - 2008
SP - 1296-1305
ST - Targeting multiple Alzheimer's disease etiologies with multimodal
neuroprotective and neurorestorative iron chelators
T2 - FASEB Journal
TI - Targeting multiple Alzheimer's disease etiologies with multimodal
neuroprotective and neurorestorative iron chelators
43249100611&doi=10.1096%2ffj.07-
8627rev&partnerID=40&md5=ffcc18ff0f62a157a049ba438ddc6d1d
VL - 22
ID - 5759
ER -
TY - JOUR
AB - The progress of nanoparticles production by eco-friendly route, with
desirable chemical and physical characteristics, and their application in helpful
fields is still under investigation. Therefore, this study aimed at biosynthesis,
characterization, and biomedical applications of silver nanoparticles (AgNPs) using
yeasts metabolite. The yeast strains, Pichia kudriavzeviiHA-NY2 and Saccharomyces
uvarumHA-NY3, were used for extracellular biosynthesis of AgNPsK and AgNPsU,
respectively. AgNPs were characterized by UV-visible spectrophotometry,
transmission electron microscopy (TEM), Fourier Transformed Infrared (FTIR)
spectrum and dynamic light scatter (DLS). TEM image showed well dispersed round and
cubic regular particles with size ranges of 12.4 +/- 6.02 nm for AgNPsU and 20.655
+/- 9.48 nm for AgNPsK. According to DLS analysis, the mean size diameters of
AgNPsU and AgNPsK were 20.3-21.5 and 29.6-30.14 nm, respectively. AgNPs showed
highly significant inhibitory activity against gram-positive bacteria (Bacillus
subtilis ATCC6633 and Staphylococcus aureus ATCC29213), gram-negative bacteria
(Pseudomonas aeruginosa ATCC27953), Candida tropicalis ATCC750, and Fusarium
oxysporium NRC21. The anti-inflammatory activity of AgNPs revealed that paw edema
was inhibited by the oral administration of the two biosynthesized silver-
nanoparticles. In addition, they showed carrageenan activity nearest to
indomethacin. All fabricated AgNPs showed a significant analgesic effect after one
hour of administration, which was comparable to aspirin. Further, both AgNPsK and
AgNPsU demonstrated a significant anticancer activity against HCT-116 (Colon cell
line) with IC50 values 0.29, 0.24 mu g ml(-1), respectively, and PC3 (Prostate cell
line) with IC50 values 0.57, 0.50 mu g ml(-1), respectively. No ulcerogenic effects
of AgNPs were detected on the rats' stomach and it was safe on the gastric profile.
AN - WOS:000610857800004
AU - Ammar, H. A.
AU - Abd El Aty, A. A.
AU - El Awdan, S. A.
C6 - JAN 2021
DA - APR
DO - 10.1007/s00449-020-02494-3
IS - 4
PY - 2021
SN - 1615-7591
1615-7605
SP - 841-854
ST - Extracellular myco-synthesis of nano-silver using the fermentable yeasts
Pichia kudriavzeviiHA-NY2 and Saccharomyces uvarumHA-NY3, and their effective
biomedical applications
T2 - BIOPROCESS AND BIOSYSTEMS ENGINEERING
TI - Extracellular myco-synthesis of nano-silver using the fermentable yeasts
VL - 44
ID - 6095
ER -
TY - JOUR
AB - The progress of nanoparticles production by eco-friendly route, with
desirable chemical and physical characteristics, and their application in helpful
fields is still under investigation. Therefore, this study aimed at biosynthesis,
characterization, and biomedical applications of silver nanoparticles (AgNPs) using
yeasts metabolite. The yeast strains, Pichia kudriavzeviiHA-NY2 and Saccharomyces
uvarumHA-NY3, were used for extracellular biosynthesis of AgNPsK and AgNPsU,
respectively. AgNPs were characterized by UV–visible spectrophotometry,
transmission electron microscopy (TEM), Fourier Transformed Infrared (FTIR)
spectrum and dynamic light scatter (DLS). TEM image showed well dispersed round and
cubic regular particles with size ranges of 12.4 ± 6.02 nm for AgNPsU and 20.655 ±
9.48 nm for AgNPsK. According to DLS analysis, the mean size diameters of AgNPsU
and AgNPsK were 20.3–21.5 and 29.6–30.14 nm, respectively. AgNPs showed highly
significant inhibitory activity against gram-positive bacteria (Bacillus subtilis
ATCC6633 and Staphylococcus aureus ATCC29213), gram-negative bacteria (Pseudomonas
aeruginosa ATCC27953), Candida tropicalis ATCC750, and Fusarium oxysporium NRC21.
The anti-inflammatory activity of AgNPs revealed that paw edema was inhibited by
the oral administration of the two biosynthesized silver-nanoparticles. In
addition, they showed carrageenan activity nearest to indomethacin. All fabricated
AgNPs showed a significant analgesic effect after one hour of administration, which
was comparable to aspirin. Further, both AgNPsK and AgNPsU demonstrated a
significant anticancer activity against HCT-116 (Colon cell line) with IC50 values
0.29, 0.24 µg ml−1, respectively, and PC3 (Prostate cell line) with IC50 values
0.57, 0.50 µg ml−1, respectively. No ulcerogenic effects of AgNPs were detected on
the rats’ stomach and it was safe on the gastric profile. © 2021, The Author(s),
under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
AU - Ammar, H. A.
AU - El Aty, A. A. A.
AU - El Awdan, S. A.
DB - Scopus
DO - 10.1007/s00449-020-02494-3
IS - 4
KW - AgNPs
Anti-inflammatory
Antimicrobial
Cytotoxicity
Yeast
Animals
Bacillus subtilis
Candida tropicalis
Carrageenan
Drug Screening Assays, Antitumor
Fermentation
Fusarium
HCT116 Cells
Humans
Indomethacin
Inhibitory Concentration 50
Light
Metal Nanoparticles
PC-3 Cells
Phylogeny
Pichia
Rats
Rats, Wistar
Saccharomyces
Scattering, Radiation
Silver
Bacteria
Bacteriology
Biochemistry
Biosynthesis
Candida
Cell culture
Metabolites
Metal nanoparticles
Particle size analysis
acetylsalicylic acid
carrageenan
indometacin
silver nanoparticle
metal nanoparticle
silver
Chemical and physical characteristics
Extracellular biosynthesis
Gram-positive bacterium
Visible spectrophotometries
analgesic activity
animal cell
animal experiment
animal model
biosynthesis
carrageenan-induced paw edema
controlled study
cytotoxicity
female
Fusarium oxysporum
HCT 116 cell line
in vitro study
male
MTT assay
nonhuman
phylogenetic tree
Pichia kudriavzevii
priority journal
rat
Saccharomyces uvarum
surface plasmon resonance
ultraviolet visible spectrophotometry
zeta potential
zone of inhibition
animal
chemistry
drug screening
fermentation
human
IC50
light
metabolism
phylogeny
radiation scattering
Wistar rat
M3 - Article
PY - 2021
SP - 841-854
ST - Extracellular myco-synthesis of nano-silver using the fermentable yeasts
T2 - Bioprocess and Biosystems Engineering
TI - Extracellular myco-synthesis of nano-silver using the fermentable yeasts
85099908307&doi=10.1007%2fs00449-020-02494-
3&partnerID=40&md5=ca8f70961c3877151b387ca09b5f729b
VL - 44
ID - 5207
ER -
TY - JOUR
AB - Cyclosporine is a calcineurin inhibitor that acts selectively on T cells. It
has been used in dermatology since 1997 for its US Food and Drug Administration
indication of psoriasis and off-label for various other inflammatory skin
conditions, including atopic dermatitis, blistering disorders, and connective
tissue diseases. In the last decade, many dermatologists have hesitated to use this
important drug in their clinical practices because of its toxicity profile. The
purpose of this article is to review the mechanism of action of cyclosporine and
its current uses and dosing schedules. It is our goal to create a framework in
which dermatologists feel comfortable and safe incorporating cyclosporine into
their prescribing regimens. Learning objectives: After completing this learning
activity, participants should be able to describe the mechanism of action of
cyclosporine, recognize the potential role of cyclosporine in dermatology and the
evidence to support this role, and incorporate cyclosporine into his or her
prescribing regimens. © 2010 by the American Academy of Dermatology, Inc.
AU - Amor, K. T.
AU - Ryan, C.
AU - Menter, A.
DB - Scopus
DO - 10.1016/j.jaad.2010.02.063
IS - 6
KW - Atopic dermatitis
Chronic urticaria
Cyclosporine
Psoriasis
Pyoderma gangrenosum
Dermatologic Agents
Dermatology
Education, Medical, Continuing
Humans
Skin Diseases
alpha interferon
azathioprine
betamethasone dipropionate
betamethasone valerate
capsaicin
cetirizine
colchicine
colecalciferol derivative
corticosteroid
cyclophosphamide
cyclosporin
cyclosporin A
dapsone
etretin
fumaric acid
gold
itraconazole
methotrexate
methylprednisolone
mycophenolic acid 2 morpholinoethyl ester
prednisolone
prednisone
rituximab
salazosulfapyridine
sulfadiazine silver
tacrolimus
triamcinolone acetonide
tumor necrosis factor inhibitor
unindexed drug
absence of side effects
acrodermatitis continua
alopecia
alopecia areata
alopecia totalis
aphthous ulcer
ascites
atopic dermatitis
Behcet disease
chronic urticaria
clinical trial
competitive inhibition
corticosteroid therapy
creatine kinase blood level
cryotherapy
dermatomyositis
diarrhea
dose response
drug dose increase
drug dose reduction
drug dose regimen
drug efficacy
drug intermittent therapy
drug mechanism
drug megadose
drug pulse therapy
drug structure
drug toxicity
drug treatment failure
edema
eospinophilic pustular folliculitis
epidermolysis bullosa acquisita
erythrodermic psoriasis
folliculitis
genital ulcer
graft rejection
Hailey Hailey disease
hirsutism
human
hypertension
immunosuppressive treatment
lichen planus
low drug dose
microemulsion
monotherapy
mouth ulcer
nephrotoxicity
pancreatitis
papular skin disease
pemphigus vulgaris
peripheral neuropathy
photodermatosis
pityriasis rubra pilaris
pompholyx
prescription
priority journal
protein phosphorylation
prurigo nodularis
psoriasis
psoriatic arthritis
pustulosis palmoplantaris
pyoderma gangrenosum
quality of life
review
Schnitzler syndrome
scleroderma
side effect
skin inflammation
solar urticaria
split thickness skin graft
suppurative hidradenitis
tachyphylaxis
therapy effect
treatment duration
ultraviolet A radiation
ultraviolet B radiation
unspecified side effect
urticaria
M3 - Review
PY - 2010
SP - 925-946
ST - The use of cyclosporine in dermatology: Part i
T2 - Journal of the American Academy of Dermatology
TI - The use of cyclosporine in dermatology: Part i
78649387833&doi=10.1016%2fj.jaad.2010.02.063&partnerID=40&md5=9f7af53d4ec158cb99928
8a62d8f45c0
VL - 63
ID - 5719
ER -
TY - JOUR
AB - The NF-kappa B family of transcription factors plays an important role in
determining cell survival during immune, inflammatory, and stress responses. NF-
kappa B activity is frequently deregulated in human cancers and is implicated in
the resistance of tumor cells to diverse anticancer agents. We studied the effects
of novel analogs of precursors of the natural product simplactone (A) on the
activity of IkB kinase and NF-kappa B. Screening of six compounds for the ability
to inhibit TNF-induced NF-kappa B activity revealed that compound SK2009 was the
most potent of these compounds in suppressing NF-kappa B activation in KBM-5
leukemic cells. Further characterization of SK2009 indicates that this newly
synthesized molecule can suppress TNF-induced I kappa B alpha kinase activation and
inhibit the expression of three NF-kappa B-dependent gene products, cyclin D1, Bcl-
2, and VEGF, in these cells. Published by Elsevier Ltd.
AN - WOS:000272892100026
AU - Anchoori, R. K.
AU - Harikumar, K. B.
AU - Batchu, V. R.
AU - Aggarwal, B. B.
AU - Khan, S. R.
DA - JAN 1
DO - 10.1016/j.bmc.2009.10.065
IS - 1
PY - 2010
SN - 0968-0896
1464-3391
SP - 229-235
ST - Inhibition of IkB kinase and NF-kappa B by a novel synthetic compound SK 2009
T2 - BIOORGANIC & MEDICINAL CHEMISTRY
TI - Inhibition of IkB kinase and NF-kappa B by a novel synthetic compound SK 2009
VL - 18
ID - 6774
ER -
TY - JOUR
AB - The review highlights the need of non-antibacterial, non-antifungal and non-
anticancer characters of metal or metal oxide nanoparticles. The usage of
nanoparticles as a part of therapeutic measures results in certain unfavourable
effects. The nanoparticles can disturb healthy gut microorganisms that may bring
about some health damages regarding pathogenic diseases, obesity, and inflammation
likewise. Even the nonspecific interactions of nanoparticles with healthy cells and
tissues can cause altered expressions of various pro-inflammatory factors and
stress related genes. This review indicates and prospect about the demand of
nanoparticles with non-antibacterial, non-antifungal and non-anticancer properties.
Such nanoparticles will be effective in various remedial and diagnostic purposes.
AN - WOS:000658972100001
AU - Ankamwar, B.
AU - Yadwade, R.
C7 - 012003
DA - MAR 1
DO - 10.1088/2632-959X/abe473
IS - 1
PY - 2021
SN - 2632-959X
ST - A review: non-antibacterial, non-antifungal and non-anticancer properties of
nanoparticles the forgotten paradigm
T2 - NANO EXPRESS
TI - A review: non-antibacterial, non-antifungal and non-anticancer properties of
nanoparticles the forgotten paradigm
VL - 2
ID - 6635
ER -
TY - JOUR
AB - Metal nanomaterials hold great potential and play an important role in
consumer products. However, the increasing use of nanomaterials has raised concern
over inadvertent exposure and potential risks for human health and the environment.
Henceforth, in vivo testing of nanoparticles and protection against its toxicity is
required. Using rat as an animal model, effect of sodium selenite (Se), an
essential trace element, on rat testes exposed to silver nanoparticles (AgNPs) was
evaluated. Male rats were treated with AgNPs (5 mg/kg/b.w) i/p or Se
(0.2 mg/kg/b.w) by gavage. AgNP administration decreased Glutathione (GSH) levels
and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione
peroxidase (GPx) and increased levels of malondialdehyde (MDA) and expression of
interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α). However,
treatment with Se increased GSH levels and activities of SOD, CAT, and GPx compared
with AgNP-treated group and decreased the level of MDA and inflammatory biomarkers
significantly (p < 0.05) as compared with AgNP-treated group. Light microscopic
analyses also revealed that AgNP induced histopathological changes in testes
tissue. Further, protection by Se on biochemical results was confirmed by
alleviation of the histopathological changes in the tissue. Results show the
adverse effects of AgNPs on the male reproductive tract, particularly
spermatogenesis, and suggest that Se possesses significant potential in reducing
AgNP-induced testicular toxicity. © 2016, Springer Science+Business Media New York.
AU - Ansar, S.
AU - Abudawood, M.
AU - Hamed, S. S.
AU - Aleem, M. M.
DB - Scopus
DO - 10.1007/s12011-016-0759-3
IS - 1
KW - Inflammation
Selenium
Testes
Toxicity
Animals
Cytokines
Humans
Male
Malondialdehyde
Metal Nanoparticles
Oxidoreductases
Rats
Rats, Wistar
Silver
Sodium Selenite
Testis
catalase
glutathione
interleukin 1beta
interleukin 6
malonaldehyde
silver nanoparticle
sodium selenite
cytokine
metal nanoparticle
oxidoreductase
silver
animal experiment
animal model
animal tissue
Article
biochemical analysis
controlled study
cytokine release
drug efficacy
drug mechanism
enzyme activity
enzyme inhibition
histopathology
in vivo study
male
microscopy
nonhuman
orchitis
oxidative stress
protection
protein expression
rat
spermatogenesis
testis disease
testis toxicity
treatment duration
animal
chemistry
human
metabolism
pathology
testis
Wistar rat
M3 - Article
PY - 2017
SP - 161-168
ST - Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular
Toxicity and Inflammation
T2 - Biological Trace Element Research
TI - Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular
Toxicity and Inflammation
84971455119&doi=10.1007%2fs12011-016-0759-
3&partnerID=40&md5=0210bce617c4555ee8a8ca6d45b14c71
VL - 175
ID - 5522
ER -
TY - JOUR
AB - Nowadays, bone diseases and defects as a result of trauma, cancers,
infections and degenerative and inflammatory conditions are increasing.
Consequently, bone repair and replacement have been developed with improvement of
orthopedic technologies and biomaterials of superior properties. This review paper
is intended to sum up and discuss the most relevant studies performed in the field
of bone biology and bone regeneration approaches. Therefore, the bone tissue
regeneration was investigated by synthetic substitutes, scaffolds incorporating
active molecules, nanomedicine, cell-based products, biomimetic fibrous and
nonfibrous substitutes, biomaterial-based three-dimensional (3D) cell-printing
substitutes, bioactive porous polymer/inorganic composites, magnetic field and
nano-scaffolds with stem cells and bone–biomaterials interface studies. © 2019, The
Author(s).
AU - Ansari, M.
DB - Scopus
DO - 10.1007/s40204-019-00125-z
IS - 4
Biology
Biomaterials
Bone regeneration
Tissue engineering
3 hydroxybutyric acid
antibiotic agent
bioceramics
biomaterial
bone morphogenetic protein 2
bone morphogenetic protein receptor
calcium ion
calcium phosphate
collagen type 1
growth differentiation factor 5
hydroxyapatite
hydroxyprogesterone caproate
magnetic nanoparticle
matrix protein
osteogenic protein 1
osteogenin
osteopontin
parathyroid hormone
polycaprolactone
polyetheretherketone
polyglactin
polyglycolic acid
polyhydroxyalkanoic acid
protein p15
selenium nanoparticle
silicate
silver nanoparticle
transforming growth factor beta1
unindexed drug
angiogenesis
biodegradability
biology
bone cell
bone development
bone disease
bone injury
bone matrix
bone regeneration
bone remodeling
bone structure
bone tissue
circulation
collagen fiber
computer assisted tomography
degeneration
dental pulp stem cell
electromagnetism
human
inflammation
mesenchymal stem cell
nanomedicine
nonhuman
orthopedics
osteocyte
priority journal
Review
static electricity
three-dimensional imaging
tissue engineering
tissue regeneration
M3 - Review
PY - 2019
SP - 223-237
ST - Bone tissue regeneration: biology, strategies and interface studies
T2 - Progress in Biomaterials
TI - Bone tissue regeneration: biology, strategies and interface studies
85126715689&doi=10.1007%2fs40204-019-00125-
z&partnerID=40&md5=d40f733c1f3394b2d6dda80048614495
VL - 8
ID - 5359
ER -
TY - JOUR
AB - Four-week-old mice, weighing about 25-35 g were divided into five groups (8
mice in each group): vehicle control, low-(0.5 g/kg), middle-(1 g/kg), high- (3
g/kg), and exceptionally high-dose (5 g/kg). After first and second weeks of
intraperitoneal exposure to AgNPs, biochemical, histopathological, and electron
microscopic ultrastructural changes were investigated. No significant changes were
observed in SGOT and ALP levels after first week of exposure, while the level of
SGPT significantly increased (p < 0.05) in 2nd week treated mice, indicating that
inflammatory of liver might be induced by high-dose (3 and 5 g/kg) of AgNPs. No
obvious changes were observed for UA and BUN in all groups of treated mice.
However, significant (p < 0.05) decrease in CR level was noticed in all groups of
treated mice only at high-dose (3 and 5 g/kg). No remarkable changes in lipid
profile were observed. Light microscopic histopathological investigation shows that
first week treatment had not perceptible effect on the cytoarchitecture on liver,
kidney, and spleen; while, second week treatment had only sporadic mild effects on
these organs. However, no ultrastructural electron microscopic changes were
observed in liver, kidney, and spleen of mice treated with 0.5, 1, and 3 g/kg of
AgNPs when sacrificed on first and second week; while, exceptionally high-dose (5
g/kg) of AgNPs resulted in slight nuclear chromatin condensation and irregularities
in nuclear membrane. The results suggested that AgNPs could be well tolerated in
mice when given intraperitoneally and no death has been found during the experiment
in any groups of treated mice. Interestingly, significant (<0.05) decrease in
glucose levels in all experiment group is suggestive of curious hypoglycemic role
of AgNPs warranting further study to explore its possible therapeutic potential in
hyperglycemic conditions as well as its mechanism of action at molecular level. (C)
2015 Wiley Periodicals, Inc.
AN - WOS:000382935400005
AU - Ansari, M. A.
AU - Khan, H. M.
AU - Khan, A. A.
AU - Alzohairy, M. A.
AU - Waseem, M.
AU - Ahmad, M. K.
AU - Mahdi, A. A.
DA - AUG
DO - 10.1002/tox.22104
IS - 8
PY - 2016
SN - 1520-4081
1522-7278
SP - 945-956
ST - Biochemical, Histopathological, and Transmission Electron Microscopic
Ultrastructural Changes in Mice After Exposure to Silver Nanoparticles
T2 - ENVIRONMENTAL TOXICOLOGY
TI - Biochemical, Histopathological, and Transmission Electron Microscopic
Ultrastructural Changes in Mice After Exposure to Silver Nanoparticles
VL - 31
ID - 6157
ER -
TY - JOUR
AB - Wounds represent a major healthcare problem especially in hospital-associated
infections where multi-drug resistant strains are often involved. Nowadays,
biomaterials with therapeutic molecules play an active role in wound healing and
infection prevention. In this work, the development of collagen hydrogels loaded
with silver nanoparticles and Cannabis sativa oil extract is described. The
presence of the silver nanoparticles gives interesting feature to the biomaterial
such as improved mechanical properties or resistance to collagenase degradation but
most important is the long-lasting antimicrobial effect. Cannabis sativa oil, which
is known for its anti-inflammatory and analgesic effects, possesses antioxidant
activity and successfully improved the biocompatibility and also enhances the
antimicrobial activity of the nanocomposite. Altogether, these results suggest that
this novel nanocomposite biomaterial is a promising alternative to common
treatments of wound infections and wound healing. © 2021 by the authors. Licensee
AU - Antezana, P. E.
AU - Municoy, S.
AU - Pérez, C. J.
AU - Desimone, M. F.
C7 - 1420
DB - Scopus
IS - 11
KW - Antimicrobial
Biomaterials
Cannabis sativa
Collagen
Wound healing
ammonia
antiinfective agent
antioxidant
biomaterial
cannabidiol
cannabigerol
cannabis sativa oil
collagen
collagen gel
collagen type 1
collagenase
dronabinol
essential oil
fibrillar collagen
hydrogel
hydroxyproline
metalloproteinase
nanocomposite
nanoparticle
silver nanoparticle
sodium borohydride
unclassified drug
analgesic activity
Article
atomic absorption spectrometry
bacterial growth
biocompatibility
biodegradability
carcinogenicity
cell proliferation
cell viability
colloid
colorimetry
controlled study
crystallization
cytotoxicity
disk diffusion
Gram negative bacterium
Gram positive bacterium
hospital infection
isotherm
MTT assay
nonhuman
oxidative stress
particle size
surface property
viscosity
wound healing
wound infection
zone of inhibition
M3 - Article
PY - 2021
ST - Collagen Hydrogels Loaded with Silver Nanoparticles and Cannabis Sativa Oil
T2 - Antibiotics
TI - Collagen Hydrogels Loaded with Silver Nanoparticles and Cannabis Sativa Oil
85122376182&doi=10.3390%2fantibiotics10111420&partnerID=40&md5=b0d5e8c76567e9152bdb
be5701185100
VL - 10
ID - 5174
ER -
TY - JOUR
AB - The present study was aimed to synthesize silver nanoparticles (AgNPs) from
the aqueous extracts of Odontosoria chinensis (L.) J. Sm. and the synthesized AgNPs
were examined for their biopotentials. The Odontosoria chinensis extracts were
added to 1 mM AgNO3 solution with different ratios viz., 0.5: 9.5, 1:9, 1.5: 8.5
and 2: 8 ratios for the reduction of Ag ions. After reduction, the AgNPs of
Odontosoria chinensis were analyzed spectroscopically for further confirmation. The
synthesized AgNPs of Odontosoria chinensis were characterized by pH, ultra violet–
visible spectroscopy (UV-Vis), Fourier transform–infra red spectroscopy (FT-IR),
scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDAX) and X-Ray
diffraction (XRD). The time taken for the complete reduction of Silver (Ag) in
solution to nanoparticle was 10 min. The O. chinensis aqueous extracts mediated
silver nanoparticles showed a broad peak with distinct absorption at around 400–420
nm and confirmed the silver nanoparticle formation. FT-IR results also confirmed
the existence of organic materials in the silver nanoparticles of O. chinensis. The
EDX spectra of AgNPs of O. chinenesis revealed the occurrence of a strong Ag peak.
The synthesis of AgNPs of O. chinenesis was confirmed with the existence of a peak
at 46.228°. The toxic potential of AgNPs of O. chinenesis showed varied percentage
mortality with the LC50 values of 134.68 μL/ 50 mL and 76.5 μL/50 mL, respectively.
The anti-inflammatory and anti-diabetic activities of aqueous and AgNPs of O.
chinenesis were statistically significant at p < 0.05 level. Conclusion: The
results demonstrated the toxicity, anti-diabetic and anti-inflammatory potential of
the studied AgNPs. The synthesized nanoparticles of Odontosoria chinensis could be
tested as an alternative to anticancer, anti-diabetic and anti-inflammatory drugs.
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Antonysamy Johnson, M. A.
AU - Shibila, T.
AU - Amutha, S.
AU - Menezes, I. R. A.
AU - Da Costa, J. G. M.
AU - Sampaio, N. F. L.
AU - Coutinho, H. D. M.
C7 - 66
DB - Scopus
DO - 10.3390/ph13040066
IS - 4
KW - Anti-diabetic
Anti-inflammatory
Cytotoxic
Odontosoria chinenesis
amylase
Odontosoria chinensis extract
plant extract
silver nanoparticle
silver nitrate
unclassified drug
antidiabetic activity
aqueous solution
Article
cell suspension
controlled study
energy dispersive X ray spectroscopy
enzyme inhibition
erythrocyte
fern
hemolysis
human
human cell
human experiment
LC50
mortality
normal human
Odontosoria chinensis
pH
synthesis
time
volunteer
X ray diffraction
M3 - Article
PY - 2020
ST - Synthesis of silver nanoparticles using odontosoria chinensis (L.) J. sm. and
evaluation of their biological potentials
T2 - Pharmaceuticals
TI - Synthesis of silver nanoparticles using odontosoria chinensis (L.) J. sm. and
evaluation of their biological potentials
85084145336&doi=10.3390%2fph13040066&partnerID=40&md5=db282cc35f1ca868226da1f641e5d
658
VL - 13
ID - 5315
ER -
TY - JOUR
AB - The increase in the usage of silica nanoparticles (SiNPs) in the industrial
and medical fields has raised concerns about their possible adverse effects on
human health. The present study aimed to investigate the potential adverse effects
of SiNPs at daily doses of 25 and 100 mg/kg body weight intraperitoneally (i.p.)
for 28 consecutive days on markers of liver damage in adult male rats. Results
revealed that SiNPs induced a marked increase in serum markers of liver damage,
including lactate dehydrogenase (LDH), alanine aminotransferase (ALAT), and
aspartate aminotransferase (ASAT). SiNPs also induced an elevation of reactive
oxygen species (ROS) production in liver, along with an increase in oxidative
stress markers (NO, MDA, PCO, and H2O2), and a decrease in antioxidant enzyme
activities (CAT, SOD, and GPx). Quantitative real-time PCR showed that SiNPs also
induced upregulation of pro-apoptotic gene expression (including Bax, p53, Caspase-
9/3) and downregulation of anti-apoptotic factors Bcl-2. Moreover,
histopathological analysis revealed that SiNPs induced hepatocyte alterations,
which was accompanied by sinusoidal dilatation, Kupffer cell hyperplasia, and the
presence of inflammatory cells in the liver. Taken together, these data showed that
SiNPs trigger hepatic damage through ROS-activated caspase signaling pathway, which
plays a fundamental role in SiNP-induced apoptosis in the liver.
AN - WOS:000659804400001
AU - Aouey, B.
AU - Boukholda, K.
AU - Gargouri, B.
AU - Bhatia, H. S.
AU - Attaai, A.
AU - Kebieche, M.
AU - Bouchard, M.
AU - Fetoui, H.
C6 - JUN 2021
DA - APR
DO - 10.1007/s12011-021-02774-3
IS - 4
PY - 2022
SN - 0163-4984
1559-0720
SP - 1688-1698
ST - Silica Nanoparticles Induce Hepatotoxicity by Triggering Oxidative Damage,
Apoptosis, and Bax-Bcl2 Signaling Pathway
T2 - BIOLOGICAL TRACE ELEMENT RESEARCH
TI - Silica Nanoparticles Induce Hepatotoxicity by Triggering Oxidative Damage,
Apoptosis, and Bax-Bcl2 Signaling Pathway
VL - 200
ID - 6457
ER -
TY - JOUR
AB - The health effects of silver nanoparticles (AgNPs) have not been well
investigated, despite AgNPs now being widely used in consumer products. We
investigated the metabolic behavior and toxicity of AgNPs in comparison to silver
nitrate (AgNO3) both in vivo and in vitro. AgNPs (20nm diameter) suspended in 1%
albumin solution or AgNO3 solution was injected into the mouse lung. Less than 1%
of the initial dose of AgNPs and more than 7% of the initial dose of AgNO3 was
recovered in the liver 4h after administration, suggesting that the ionic form of
silver was absorbed by the lung tissue and entered the systemic circulation more
efficiently than AgNPs. The pro-inflammatory cytokine, IL-1β, and neutrophils in
bronchoalveolar lavage fluid (BALF) increased following intratracheal instillation
of AgNPs or AgNO3. AgNO3 recruited more neutrophils in the alveolar space than did
AgNPs. In the in vitro study, AgNO3 was more cytotoxic than 20, 60, or 100nm
diameter AgNPs in a mouse macrophage cell line (J774.1). To investigate the
intracellular distribution of Ag in detail, J774.1 cells were exposed to AgNO3 or
20nm AgNPs and the distribution of Ag to cytosolic proteins was investigated using
HPLC-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). Ag was mainly
distributed to metallothioneins (MT) and to high molecular weight proteins in
AgNO3- and AgNPs-exposed cells, respectively. Confocal laser microscopic
examination of LysoTracker®-labeled cells indicated that AgNPs were colocalized
with lysosomes in J774.1 cells. These results suggest that AgNPs were transported
to lysosomes and only gradually dissolved in the macrophages, causing milder
inflammatory stimulation in the mouse lung compared to AgNO3. © 2014 Elsevier
Ireland Ltd.
AU - Arai, Y.
AU - Miyayama, T.
AU - Hirano, S.
DB - Scopus
DO - 10.1016/j.tox.2014.12.014
KW - Lung
Lysosome
Macrophages
Metallothionein
Silver ion
Silver nanoparticle
Animals
Biological Transport
Bronchoalveolar Lavage Fluid
Cell Line
Cell Survival
Inhalation Exposure
Interleukin-1beta
Liver
Lysosomes
Male
Metal Nanoparticles
Mice, Inbred ICR
Molecular Weight
Neutrophil Infiltration
Neutrophils
Particle Size
Silver Nitrate
Time Factors
albumin
interleukin 1beta
ion
metallothionein
silver nanoparticle
silver nitrate
autacoid
IL1B protein, mouse
metal nanoparticle
animal experiment
animal tissue
Article
cellular distribution
comparative study
controlled study
cytotoxicity
in vitro study
in vivo study
liver
lung lavage
lung parenchyma
lysosome
macrophage
male
mass spectrometry
microscopy
molecular weight
mouse
neutrophil
nonhuman
systemic circulation
tissue distribution
toxicity testing
animal cell
animal model
cell lysate
confocal laser microscopy
cytosol
cytosolic fraction
hydrodynamics
liver weight
lung
lung alveolus macrophage
lung toxicity
particle size
protein localization
zeta potential
adverse effects
animal
cell line
cell survival
cytology
dose response
drug effects
exposure
immunology
Institute for Cancer Research mouse
metabolism
pathology
time
transport at the cellular level
M3 - Article
PY - 2015
SP - 84-92
ST - Difference in the toxicity mechanism between ion and nanoparticle forms of
silver in the mouse lung and in macrophages
T2 - Toxicology
TI - Difference in the toxicity mechanism between ion and nanoparticle forms of
silver in the mouse lung and in macrophages
84919897809&doi=10.1016%2fj.tox.2014.12.014&partnerID=40&md5=56cbb81b5968cc29df9edf
01245905b4
VL - 328
ID - 5572
ER -
TY - JOUR
AB - Neural interfaces enabling light transmittance rely on optogenetics to
control and monitor specific neural activity, thereby facilitating deeper
understanding of intractable diseases. This study reports the material strategy
underlying an optogenetic neural interface comprising stretchable and transparent
conductive tracks and capable of demonstrating high biocompatibility after long-
term (5-month) implantation. Ag/Au core-shell nanowires contribute toward improving
track performance in terms of stretchability (<60% strain), transparency (<83%),
and electrical resistance (15 omega sq(-1)). The neural interface integrated with
gel-coated exterior microelectrodes preserves low impedance (1.1-3.2 omega cm(2))
in a saline solution over the evaluated 5-month period. Besides the use of
efficient conductive materials, surface treatment using antithrombogenic polymer
tends to prevent the growth of granulation tissue, thereby facilitating clear
monitoring of electrocorticograms (ECoG) in a rodent during chronic implantation.
The flexible and transparent neural interface pathologically exhibits
noncytotoxicity and low inflammatory response while efficiently recording evoked
ECoG in a nonhuman primate via optogenetic stimulation. The proposed highly
reliable interface can be employed in multifaceted approaches for translational
research based on chronic implants.
AN - WOS:000468803300013
AU - Araki, T.
AU - Yoshida, F.
AU - Uemura, T.
AU - Noda, Y.
AU - Yoshimoto, S.
AU - Kaiju, T.
AU - Suzuki, T.
AU - Hamanaka, H.
AU - Baba, K.
AU - Hayakawa, H.
AU - Yabumoto, T.
AU - Mochizuki, H.
AU - Kobayashi, S.
AU - Tanaka, M.
AU - Hirata, M.
AU - Sekitani, T.
C7 - 1900130
DA - MAY 23
DO - 10.1002/adhm.201900130
IS - 10
PY - 2019
SN - 2192-2640
2192-2659
ST - Long-Term Implantable, Flexible, and Transparent Neural Interface Based on
Ag/Au Core-Shell Nanowires
T2 - ADVANCED HEALTHCARE MATERIALS
TI - Long-Term Implantable, Flexible, and Transparent Neural Interface Based on
Ag/Au Core-Shell Nanowires
VL - 8
ID - 6741
ER -
TY - JOUR
AB - Neural interfaces enabling light transmittance rely on optogenetics to
control and monitor specific neural activity, thereby facilitating deeper
understanding of intractable diseases. This study reports the material strategy
underlying an optogenetic neural interface comprising stretchable and transparent
conductive tracks and capable of demonstrating high biocompatibility after long-
term (5-month) implantation. Ag/Au core–shell nanowires contribute toward improving
track performance in terms of stretchability (<60% strain), transparency (<83%),
and electrical resistance (15 Ω sq−1). The neural interface integrated with gel-
coated exterior microelectrodes preserves low impedance (1.1–3.2 Ω cm2) in a saline
solution over the evaluated 5-month period. Besides the use of efficient conductive
materials, surface treatment using antithrombogenic polymer tends to prevent the
growth of granulation tissue, thereby facilitating clear monitoring of
electrocorticograms (ECoG) in a rodent during chronic implantation. The flexible
and transparent neural interface pathologically exhibits noncytotoxicity and low
inflammatory response while efficiently recording evoked ECoG in a nonhuman primate
via optogenetic stimulation. The proposed highly reliable interface can be employed
in multifaceted approaches for translational research based on chronic implants. ©
2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
AU - Araki, T.
AU - Yoshida, F.
AU - Uemura, T.
AU - Noda, Y.
AU - Yoshimoto, S.
AU - Kaiju, T.
AU - Suzuki, T.
AU - Hamanaka, H.
AU - Baba, K.
AU - Hayakawa, H.
AU - Yabumoto, T.
AU - Mochizuki, H.
AU - Kobayashi, S.
AU - Tanaka, M.
AU - Hirata, M.
AU - Sekitani, T.
C7 - 1900130
DB - Scopus
DO - 10.1002/adhm.201900130
IS - 10
KW - electrocorticograms
flexible electronics
nanowires
optogenetics
transparent electrodes
Animals
Cerebral Cortex
Electric Impedance
Electrocorticography
Electrodes, Implanted
Evoked Potentials, Somatosensory
Gold
Nanowires
Optogenetics
Polyvinyl Alcohol
Rats
Silver
Biocompatibility
Conductive materials
Disease control
Electrophysiology
Flexible electronics
Microelectrodes
Neurons
Surface treatment
acrylic acid butyl ester
copolymer
fibrinolytic agent
gold nanoparticle
nanoshell
nanowire
poly(3 methoxypropyl acrylate)
polymer
silver nanoparticle
sodium chloride
stearic acid
unclassified drug
gold
polyvinyl alcohol
silver
Chronic implantation
Electrical resistances
Inflammatory response
Multi-faceted approach
Translational Research
Transparent conductive
animal experiment
Article
biocompatibility
brain computer interface
Callitrichinae
controlled study
electric conductance
electric resistance
electrocorticography
granulation tissue
hydrogel
impedance
implantation
inflammation
material coating
nanofabrication
neural interface
nonhuman
optics
priority journal
rat
somatosensory evoked potential
stress strain relationship
surface property
validation study
animal
brain cortex
chemistry
electrode implant
physiology
procedures
Transparent electrodes
M3 - Article
PY - 2019
ST - Long-Term Implantable, Flexible, and Transparent Neural Interface Based on
Ag/Au Core–Shell Nanowires
T2 - Advanced Healthcare Materials
TI - Long-Term Implantable, Flexible, and Transparent Neural Interface Based on
Ag/Au Core–Shell Nanowires
85063881948&doi=10.1002%2fadhm.201900130&partnerID=40&md5=95fc2ee12ab47dfb40edf9885
42f4791
VL - 8
ID - 5408
ER -
TY - JOUR
AB - Lately, nickel oxide nanoparticles (NiO NPs) have been employed in different
industrial and biomedical fields. Several studies have reported that NiO NPs may
affect the development of reproductive organs inducing oxidative stress and,
resulting in male infertility. We investigated the in vitro effects of NiO NPs on
porcine pre-pubertal Sertoli cells (SCs) which undergone acute (24 h) and chronic
(from 1 up to 3 weeks) exposure at two subtoxic doses of NiO NPs of 1 mu g/ml and 5
mu g/ml. After NiO NPs exposure we performed the following analysis: (a) SCs
morphological analysis (Light Microscopy); (b) ROS production and oxidative DNA
damage, gene expression of antioxidant enzymes (c) SCs functionality (AMH, inhibin
B Real-time PCR analysis and ELISA test); (d) apoptosis (WB analysis); (e) pro-
inflammatory cytokines (Real-time PCR analysis), and (f) MAPK kinase signaling
pathway (WB analysis). We found that the SCs exposed to both subtoxic doses of NiO
NPs didn't sustain substantial morphological changes. NiO NPs exposure, at each
concentration, reported a marked increase of intracellular ROS at the third week of
treatment and DNA damage at all exposure times. We demonstrated, un up-regulation
of SOD and HO-1 gene expression, at both concentrations tested. The both subtoxic
doses of NiO NPs detected a down-regulation of AMH and inhibin B gene expression
and secreted proteins. Only the 5 mu g/ml dose induced the activation of caspase-3
at the third week. At the two subtoxic doses of NiO NPs a clear pro-inflammatory
response was resulted in an up-regulation of TNF-alpha and IL-6 in terms of mRNA.
Finally, an increased phosphorylation ratio of p-ERK1/2, p-38 and p-AKT was
observed up to the third week, at both concentrations. Our results show the
negative impact of subtoxic doses NiO NPs chronic exposure on porcine SCs
functionality and viability.
AN - WOS:000968199300001
AU - Arato, I.
AU - Giovagnoli, S.
AU - Di Michele, A.
AU - Bellucci, C.
AU - Lilli, C.
AU - Aglietti, M. C.
AU - Bartolini, D.
AU - Gambelunghe, A.
AU - Muzi, G.
AU - Calvitti, M.
AU - Eugeni, E.
AU - Gaggia, F.
AU - Baroni, T.
AU - Mancuso, F.
AU - Luca, G.
C7 - 1063916
DA - MAR 30
DO - 10.3389/fendo.2023.1063916
PY - 2023
SN - 1664-2392
ST - Nickel oxide nanoparticles exposure as a risk factor for male infertility:
"In vitro" effects on porcine pre-pubertal Sertoli cells
T2 - FRONTIERS IN ENDOCRINOLOGY
TI - Nickel oxide nanoparticles exposure as a risk factor for male infertility:
"In vitro" effects on porcine pre-pubertal Sertoli cells
VL - 14
ID - 6591
ER -
TY - JOUR
AB - Introducción: Los fibroblastos gingivales humanos (FGH) tienen un papel
importante en la enfermedad periodontal, pues alteran su normal funcionamiento en
respuesta a estímulos pro-inflamatorios. Se cree que los fibroblastos se pueden
eliminar anormalmente por medio de apoptosis en periodontitis. El propósito de este
estudio es determinar y cuantificar la apoptosis de FGH en biopsias del periodonto
de individuos sanos y con enfermedad periodontal. Métodos: Se realizó un estudio
clínico descriptivo de corte transversal en personas con diagnóstico de salud
periodontal (S), gingivitis (G) y periodontitis crónica (PC). Se tomaron biopsias
escisionales y se hicieron tinciones inmunohistoquímicas (hematoxilina-eosina,
caspasa-3 y vimentina). Las placas se interpretaron por histopatología y se
digitalizaron para cuantificar las células apoptóticas. Todos los datos se
analizaron con un software estadístico para encontrar diferencias significativas
(p<0.05). Resultados: La población celular total de fibroblastos tuvo un promedio
de 430±67.6 en los individuos sanos y una disminución significativamente progresiva
en gingivitis (270±37.1) y periodontitis crónica (206.5±69.8) (p<0.05). La
expresión de fibroblastos apoptóticos por campo aumentó de acuerdo con las
severidades de la enfermedad [28±16 en sanos (6.5%); 31±17 en gingivitis (11.5%) y
51±24 en periodontitis (24.8%.), (p<0.001)]. La relación entre la expresión de
fibroblastos apoptóticos y la profundidad de la bolsa periodontal no fue
significante (p>0.5, r²=0.02); mientras que para las células inflamatorias se
encontró una relación proporcional significativa (p<0.05, r²=0.2018).
Introduction: Human gingival fibroblasts (HGF) have an important role in the
periodontal immune response. The fibroblasts alter their normal behavior in
response to pro-inflammatory cytokines. It is believed that HGF can be diminished
and/or eliminated by means of apoptosis. The purpose of this study was to determine
and to quantify apoptosis of HGF in periodontium biopsies from healthy and chronic
periodontitis patients. Methods: A clinical cross-sectional study in people with
healthy periodontium (S), gingivitis (G) and chronic periodontitis (PC) patients
was carried out. The periodontal biopsies were obtained and immunostained by means
of: hematoxylin-eosin, caspase-3, vimentin and caspase-vimentin double-staining for
specific visualization of apoptotic fibroblasts. Histopathological and digital
analyses were performed. Descriptive statistics were applied to categorical and
nominal variables. Results: Total cell population of HGF had an average of 430±67.6
cells/field in healthy people, and a significantly progressive decrease in
gingivitis (270±37.1) and chronic periodontitis groups (206.5±69.8) (p<0.05). As
for total population of inflammatory cells, an increase was noticed in gingivitis
(191.8±50.1) and a decrease in periodontitis (109.3±21.7) without statistical
significance. The expression of apoptotic HGF per field increased accordingly to
the severity of the disease [28±16 in health (6.5%); 31±17 in gingivitis (11.5%)
and 51±24 in periodontitis (24.8%), p<0.001]. Similar findings were observed for
inflammatory cells with different percentage expression [17±13 in health (23%);
28±19 in gingivitis (14.6%) and 47±35 in periodontitis (43.1%), p<0.05]. The
relationship between the percentage of expression of apoptotic cells and probing
pocket depth was proportional but not significant (p>0.5, r²=0.02); while for the
inflammatory cells a significant relationship was observed (p<0.05, r²=0.2018).
AD - Arce, Roger Mauricio
Universidad del Valle. Facultad de Salud. Escuela de Odontología. Santiago de Cali.
CO
Tamayo, Oscar
Universidad Santiago de Cali. Facultad de Medicina. Santiago de Cali. CO
Cortés, Armando
Universidad del Valle. Facultad de Salud. Escuela de Medicina. Santiago de Cali. CO
AU - Arce, Roger Mauricio
AU - Tamayo, Oscar
AU - Cortés, Armando
C1 - 20110131
DA - 2007/09
DB - LILACS
IS - 3
KW - Apoptosis
Fibroblasts
Gingival
Gingivitis
Immunohistochemistry
Inmunohistoquímica
Pathogenesis
Patogénesis
Periodontitis
LA - es
PY - 2007
SN - 0120-8322
SP - 197-209
ST - Apoptosis de fibroblastos gingivales en periodontitis
T2 - Colomb. med
TI - Apoptosis de fibroblastos gingivales en periodontitis
TT - Apoptosis of gingival fibroblasts in periodontitis
UR - http://colombiamedica.univalle.edu.co/index.php/comedica/article/view/505/984
VL - 38
ID - 4953
ER -
TY - JOUR
AB - Bacterial nanocellulose (BNC) is a novel nanomaterial known for its large
surface area, biocompatibility, and non-toxicity. BNC contributes to regenerative
processes in the skin but lacks antimicrobial and anti-inflammatory properties.
Herein, the development of bioactive wound dressings by loading antibacterial
povidone-iodine (PVI) or anti-inflammatory acetylsalicylic acid (ASA) into
bacterial cellulose is presented. BNC is produced using Hestrin-Schramm culture
media and loaded via immersion in PVI and ASA. Through scanning electron
microscopy, BNC reveals open porosity where the bioactive compounds are loaded; the
mechanical tests show that the dressing prevents mechanical wear. The loading
kinetic and release assays (using the Franz cell method) under simulated fluids
present a maximum loading of 589.36 mg PVI/g BNC and 38.61 mg ASA/g BNC, and both
systems present a slow release profile at 24 h. Through histology, the complete
diffusion of the bioactive compounds is observed across the layers of porcine skin.
Finally, in the antimicrobial experiment, BNC/PVI produced an inhibition halo for
Gram-positive and Gram-negative bacteria, confirming the antibacterial activity.
Meanwhile, the protein denaturation test shows effective anti-inflammatory activity
in BNC/ASA dressings. Accordingly, BNC is a suitable platform for the development
of bioactive wound dressings, particularly those with antibacterial and anti-
inflammatory properties.
AN - WOS:000845785000001
AU - Argel, S.
AU - Castano, M.
AU - Jimenez, D. E.
AU - Rodriguez, S.
AU - Vallejo, M. J.
AU - Castro, C. I.
AU - Osorio, M. A.
C7 - 1661
DA - AUG
IS - 8
PY - 2022
SN - 1999-4923
ST - Assessment of Bacterial Nanocellulose Loaded with Acetylsalicylic Acid or
Povidone-Iodine as Bioactive Dressings for Skin and Soft Tissue Infections
T2 - PHARMACEUTICS
TI - Assessment of Bacterial Nanocellulose Loaded with Acetylsalicylic Acid or
Povidone-Iodine as Bioactive Dressings for Skin and Soft Tissue Infections
VL - 14
ID - 6352
ER -
TY - JOUR
AB - The aim of the present review article was to analyze the biological impact
and toxicology of titanium dioxide nanoparticles (TiO2 NPs) to determine their
cytotoxicity, reactive oxygen species production as well as induction of oxidative
stress through a systematic review of the literature published so far about the
biocompatibility of TiO2 NPs in contact with oral cells. Available data on
nanoparticles (NPs) were collected from the PubMed and Science Direct electronic
databases, and other sources according to PRISMA recommendations for systematic
reviews. In the qualitative analysis of published data on cytotoxicity in oral
cells, a slight increase in the number of metabolically active cells has been
observed when the TiO2 NPs are in contact with oral cells; however, at certain
doses cellular viability decreases significantly. One of the negative effects of
NPs is the induction of prostaglandin E-2 production in a previous pro-inflammatory
state, which could increase the existing inflammatory effect. Therefore, the
biological application of these nanoparticles must be performed both in vivo and
metabolomic studies, since it is possible that the results of the in vitro
experiments cannot be extrapolated to an in vivo system, since it been observed
that the particles tend to aggregated among them, in the culture medium, used for
such as tests. Therefore, although it can be considered a material with light or
even biocompatible cytotoxicity, it must not be considered completely harmless.
AN - WOS:000538545400007
AU - Argueta-Figueroa, L.
AU - Torres-Gomez, N.
AU - Scougall-Vilchis, R. J.
AU - Garcia-Contreras, R.
DO - 10.22209/IC.v59n4a06
IS - 4
PY - 2018
SN - 0535-5133
SP - 352-368
ST - Biocompatibility and nanotoxicology of titanium dioxide in the oral cavity:
Systematic review
T2 - INVESTIGACION CLINICA
TI - Biocompatibility and nanotoxicology of titanium dioxide in the oral cavity:
Systematic review
VL - 59
ID - 6197
ER -
TY - JOUR
AB - Owing to the serious adverse effects caused by pyrimethamine and
sulfadiazine, the drugs commonly used to treat toxoplasmosis, there is a need for
treatment alternatives for this disease. Nanotechnology has enabled significant
advances toward this goal. This study was conducted to evaluate the activity of
biogenic silver nanoparticles (AgNp-Bio) in RAW 264.7 murine macrophages infected
with the RH strain of Toxoplasma gondii. The macrophages were infected with T.
gondii tachyzoites and then treated with various concentrations of AgNp-Bio. The
cells were evaluated by microscopy, and culture supernatants were collected for
ELISA determination of their cytokine concentration. Treatment with 6 μM AgNp-Bio
reduced the infection and parasite load in infected RAW 264.7 macrophages without
being toxic to the cells. The treatment also induced the synthesis of reactive
oxygen species and tumor necrosis factor-alpha (both pro-inflammatory mediators),
which resulted in ultrastructural changes in the tachyzoites and their
intramacrophagic destruction. Our findings suggest that AgNp-Bio affect T. gondii
tachyzoites by activating microbicidal and pro-inflammatory mechanisms and may be a
potential alternative treatment for toxoplasmosis. © 2022 Institut Pasteur
AU - Arruda da Silva Sanfelice, R.
AU - Silva, T. F.
AU - Tomiotto-Pellissier, F.
AU - Bortoleti, B. T. D. S.
AU - Lazarin-Bidóia, D.
AU - Scandorieiro, S.
AU - Nakazato, G.
AU - de Barros, L. D.
AU - Garcia, J. L.
AU - Verri, W. A.
AU - Conchon-Costa, I.
AU - Pavanelli, W. R.
AU - Costa, I. N.
C7 - 104971
DB - Scopus
DO - 10.1016/j.micinf.2022.104971
IS - 5
KW - Biogenic silver nanoparticles (AgNp-Bio)
Cytotoxicity
RAW 264.7 cells
Toxoplasmosis
Treatment
Animals
Cell Proliferation
Macrophages
Metal Nanoparticles
Mice
Silver
Toxoplasma
interleukin 10
pyrimethamine
silver nanoparticle
sulfadiazine
metal nanoparticle
silver
animal cell
antiparasitic activity
apicoplast
Article
cell proliferation
cell vacuole
cell viability
comparative study
controlled study
cytokine production
immunomodulation
microneme
microscopy
mitochondrion
mouse
nonhuman
parasite load
RAW 264.7 cell line
supernatant
tachyzoite
toxoplasmosis
ultrastructure
animal
macrophage
M3 - Article
PY - 2022
ST - Biogenic silver nanoparticles reduce Toxoplasma gondii infection and
proliferation in RAW 264.7 macrophages by inducing tumor necrosis factor-alpha and
reactive oxygen species production in the cells
T2 - Microbes and Infection
TI - Biogenic silver nanoparticles reduce Toxoplasma gondii infection and
85133168680&doi=10.1016%2fj.micinf.2022.104971&partnerID=40&md5=094e4f3a85a8fe95fd4
56dc5678f44e3
VL - 24
ID - 5155
ER -
TY - JOUR
AB - Stroke is among the three leading causes of death worldwide and the most
frequent cause of permanent disability. Brain ischemia induces an inflammatory
response involving activated complement fragments. Here we show that i.v. Ig (IVIG)
treatment, which scavenges complement fragments, protects brain cells against the
deleterious effects of experimental ischemia and reperfusion (I/R) and prevents
I/R-induced mortality in mice. Animals administered IVIG either 30 min before
ischemia or after 3 h of reperfusion exhibited a 50-60% reduction of brain infarct
size and a 2- to 3-fold improvement of the functional outcome. Even a single low
dose of IVIG given after stroke was effective. IVIG was protective in the
nonreperfusion model of murine stroke as well and did not exert any peripheral
effects. Human IgG as well as intrinsic murine C3 levels were significantly higher
in the infarcted brain region compared with the noninjured side, and their physical
association was demonstrated by immuno-coprecipitation. C5-deficient mice were
significantly protected from I/R injury compared with their wild-type littermates.
Exposure of cultured neurons to oxygen/glucose deprivation resulted in increased
levels of C3 associated with activation of caspase 3, a marker of apoptosis; both
signals were attenuated with IVIG treatment. Our data suggest a major role for
complement-mediated cell death in ischemic brain injury and the prospect of using
IVIG in relatively low doses as an interventional therapy for stroke.
AN - WOS:000249187500045
AU - Arumugam, T. V.
AU - Tang, S. C.
AU - Lathia, J. D.
AU - Cheng, A.
AU - Mughal, M. R.
AU - Chigurupati, S.
AU - Magnus, T.
AU - Chan, S. L.
AU - Jo, D. G.
AU - Ouyang, X.
AU - Fairlie, D. P.
AU - Granger, D. N.
AU - Vortmeyer, A.
AU - Basta, M.
AU - Mattson, M. P.
DA - AUG 28
DO - 10.1073/pnas.0700506104
IS - 35
PY - 2007
SN - 0027-8424
SP - 14104-14109
ST - Intravenous immunoglobulin (IVIG) protects the brain against experimental
stroke by preventing complement-mediated neuronal cell death
T2 - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA
TI - Intravenous immunoglobulin (IVIG) protects the brain against experimental
stroke by preventing complement-mediated neuronal cell death
VL - 104
ID - 6584
ER -
TY - JOUR
AB - Exposure to blast overpressure waves is implicated as the major cause of
ocular injuries and resultant visual dysfunction in veterans involved in recent
combat operations. No effective therapeutic strategies have been developed so far
for blast-induced ocular dysfunction. Lysophosphatidic acid (LPA) is a bioactive
phospholipid generated by activated platelets, astrocytes, choroidal plexus cells,
and microglia and is reported to play major roles in stimulating inflammatory
processes. The levels of LPA in the cerebrospinal fluid have been reported to
increase acutely in patients with traumatic brain injury (TBI) as well as in a
controlled cortical impact (CCI) TBI model in mice. In the present study, we have
evaluated the efficacy of a single intravenous administration of a monoclonal LPA
antibody (25 mg/kg) given at 1 h post-blast for protection against injuries to the
retina and associated ocular dysfunctions. Our results show that a single 19 psi
blast exposure significantly increased the levels of several species of LPA in
blood plasma at 1 and 4 h post-blast. The anti-LPA antibody treatment significantly
decreased glial cell activation and preserved neuronal cell morphology in the
retina on day 8 after blast exposure. Optokinetic measurements indicated that anti-
LPA antibody treatment significantly improved visual acuity in both eyes on days 2
and 6 post-blast exposure. Anti-LPA antibody treatment significantly increased rod
photoreceptor and bipolar neuronal cell signaling in both eyes on day 7 post-blast
exposure. These results suggest that blast exposure triggers release of LPAs, which
play a major role promoting blast-induced ocular injuries, and that a single early
administration of anti-LPA antibodies provides significant protection.
AN - WOS:000603039900001
AU - Arun, P.
AU - Rossetti, F.
AU - DeMar, J. C.
AU - Wang, Y.
AU - Batuure, A. B.
AU - Wilder, D. M.
AU - Gist, I. D.
AU - Morris, A. J.
AU - Sabbadini, R. A.
AU - Long, J. S. B.
C7 - 611816
DA - DEC 15
DO - 10.3389/fneur.2020.611816
PY - 2020
SN - 1664-2295
ST - Antibodies Against Lysophosphatidic Acid Protect Against Blast-Induced Ocular
Injuries
T2 - FRONTIERS IN NEUROLOGY
TI - Antibodies Against Lysophosphatidic Acid Protect Against Blast-Induced Ocular
Injuries
VL - 11
ID - 6763
ER -
TY - JOUR
AB - Despite an increasing surge in application of nanoparticles in industries,
there is a serious lack of information concerning their impact on human health and
the environment. The present study investigated effects of molybdenum nanoparticles
(Mo NPs) injected intraperitoneally into Sprague-Dawley rats at different doses of
Mo NPs (5, 10, and 15 mg/kg BW per day) during a period of 28 days. Hematological
and biochemical parameters as well as sexual hormones and histopathological
examinations of the liver and testis were assessed and compared with control group.
The results showed that the serum levels of testosterone decreased significantly in
both groups of 10 and 15 mg (Mo NPs)/kg BW in comparison with the control group (p
< 0.05). However, there were insignificant differences observed in luteinizing
hormone (LH) levels and hematological parameters when compared with the control
group (p > 0.05). The results of liver enzymes showed that serum levels of
aspartate aminotransferase (AST) decreased significantly in both dosage groups of 5
and 10 mg/kg BW (Mo NPs) when compared with the control group (p < 0.05), and
significant decrease obtained in lactate dehydrogenase (LDH) levels at dose of 5
mg/kg BW in comparison with the control group (p < 0.05). The histopathological
examination of testis showed a decrease in number of Leydig cells. Also, the number
of chronic inflammatory cells increased in portal triad and parenchyma in liver
tissue of rats exposed to Mo NPs.
AN - WOS:000392194700007
AU - Asadi, F.
AU - Mohseni, M.
AU - Noshahr, K. D.
AU - Soleymani, F. H.
AU - Jalilvand, A.
AU - Heidari, A.
DA - JAN
DO - 10.1007/s12011-016-0765-5
IS - 1
PY - 2017
SN - 0163-4984
1559-0720
SP - 50-56
ST - Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual
Hormones in Male Rats
TI - Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual
Hormones in Male Rats
VL - 175
ID - 6623
ER -
TY - JOUR
AB - We recently demonstrated that a new PG1828-encoded lipoprotein (PG1828LP) was
able to be separated from a Porphyromonas gingivalis lipopolysaccharide (LPS)
preparation, and we found that it exhibited strong cell activation, similar to that
of Escherichia coli LPS, through a Toll-like receptor 2 (TLR2)-dependent pathway.
In order to determine the virulence of PG1828LP toward cell activation, we
generated a PG1828-deficient mutant of P. gingivalis strain 381 by allelic exchange
mutagenesis using an ermF-ermAM antibiotic resistance cassette. A highly purified
preparation of LPS from a PG1828-deficient mutant (APG1828-LPS) showed nearly the
same ladder-like patterns in silver-stained gels as a preparation of LPS from a
wild-type strain (WT-LPS), as well as Limulus amoebocyte lysate activities that
were similar to those of the WT-LPS preparation. However, the ability of the
APG1828-LPS preparation to activate NF-êÂ in TLR2-expressing cells was markedly
attenuated. Cytokine production by human gingival fibroblasts was also decreased in
response to the APG1828-LPS preparation in comparison with the WT-LPS preparation,
and the activity was comparable to the stimulation of highly purified lipid A of P.
gingivalis by TLR4. Further, lethal toxicity was rarely observed following
intraperitoneal injection of the PG1828-deficient mutant into mice compared to that
with the wild-type strain, while the APG1828-LPS preparation showed no lethal
toxicity. Taken together, these results clearly indicate that PG1828LP plays an
essential role in inflammatory responses and may be a major virulence factor of P.
gingivalis. Copyright © 2005, American Society for Microbiology. All Rights
Reserved.
AU - Asai, Y.
AU - Hashimoto, M.
AU - Fletcher, H. M.
AU - Miyake, K.
AU - Akira, S.
AU - Ogawa, T.
DB - Scopus
DO - 10.1128/IAI.73.4.2157-2163.2005
IS - 4
KW - Animals
Humans
Lipopolysaccharides
Lipoproteins
Male
Mice
Mice, Inbred BALB C
Porphyromonas gingivalis
Receptors, Cell Surface
Signal Transduction
Toll-Like Receptor 2
Virulence Factors
lipoprotein
toll like receptor 2
unclassified drug
virulence factor
virulence factor pg1828lp
cell surface receptor
lipopolysaccharide
Tlr2 protein, mouse
animal cell
animal model
antibiotic resistance
article
bacterial virulence
cell activation
controlled study
cytokine production
enzyme activity
fibroblast
gene cassette
gingiva
Limulus
male
mouse
mutagenesis
nonhuman
nucleotide sequence
priority journal
protein expression
signal transduction
animal
Bagg albino mouse
human
pathogenicity
physiology
M3 - Article
PY - 2005
SP - 2157-2163
ST - Lipopolysaccharide preparation extracted from Porphyromonas gingivalis
lipoprotein-deficient mutant shows a marked decrease in toll-like receptor 2-
mediated signaling
T2 - Infection and Immunity
TI - Lipopolysaccharide preparation extracted from Porphyromonas gingivalis
lipoprotein-deficient mutant shows a marked decrease in toll-like receptor 2-
mediated signaling
16244411282&doi=10.1128%2fIAI.73.4.2157-
2163.2005&partnerID=40&md5=9db271ade42b17e30954249549c98516
VL - 73
ID - 5775
ER -
TY - JOUR
AB - Purpose: The current study proposed the simple, eco-friendly and cost-
effective synthesis of carboxymethyl cellulose (CMC) structured silver-based
nanocomposite (CMC-AgNPs) using Syzygium aromaticum buds extract. Methods: The CMC-
AgNPs were characterized by ultraviolet (UV) spectroscopy, scanning electron
microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD),
Fourier transmission infra-red (FTIR), energy-dispersive X-ray (EDX), and dynamic
light scattering (DLS) techniques. The synthesized nanocomposites were evaluated
for their bactericidal kinetics, in-vivo anti-inflammatory, anti-leishmaniasis,
antioxidant and cytotoxic activities using different in-vitro and in-vivo models.
Results: The spherical shape nanocomposite of CMC-AgNPs was synthesized with the
mean size range of 20–30 nm, and the average pore diameter is 18.2 nm while the
mean zeta potential of −31.6 ± 3.64 mV. The highly significant (P < 0.005)
antibacterial activity was found against six bacterial strains with the ZIs of 24.6
to 27.9 mm. More drop counts were observed in Gram-negative strains after 10 min
exposure with CMC-AgNPs. Significant damage in bacterial cell membrane was also
observed in atomic force microscopy (AFM) after treated with CMC-AgNPs.
Nanocomposite showed highly significant anti-inflammatory activity in cotton pellet
induced granuloma model (Phase I) in rats with the mean inhibitions of 43.13% and
48.68% at the doses of 0.025 and 0.05 mg/kg, respectively, when compared to
control. Reduction in rat paw edema (Phase II) was also highly significant (0.025
mg/kg; 42.39%; 0.05 mg/kg, 47.82%). At dose of 0.05 mg/kg, CMC-AgNPs caused highly
significant decrease in leukocyte counts (922 ± 83), levels of CRP (8.4 ± 0.73
mg/mL), IL-1 (177.4 ± 21.3 pg/mL), IL-2 (83.7 ± 11.5 pg/mL), IL-6 (83.7 ± 11.5
pg/mL) and TNF-α (18.3 ± 5.3 pg/mL) as compared to control group. CMC-AgNPs
produced highly effective anti-leishmaniasis activity with the viable Leishmania
major counts decreased up to 36.7% within 24 h, and the IC50 was found to be 28.41
μg/mL. The potent DPPH radical scavenging potential was also observed for CMC-AgNPs
with the IC50 value of 112 μg/mL. Furthermore, the cytotoxicity was assessed using
HeLa cell lines with the LC50 of 108.2 μg/mL. Conclusion: The current findings
demonstrate positive attributes of CMC fabricated AgNPs as a promising
antibacterial, anti-inflammatory, anti-leishmaniasis, and antioxidant agent with
low cytotoxic potential. © 2021 Asghar et al.
AU - Asghar, M. A.
AU - Yousuf, R. I.
AU - Shoaib, M. H.
AU - Zehravi, M.
AU - Rehman, A. A.
AU - Imtiaz, M. S.
AU - Khan, K.
DB - Scopus
DO - 10.2147/IJN.S321419
KW - Biological applications
Carboxymethyl cellulose
Green synthesis
Syzygium aromaticum
Animals
Carboxymethylcellulose Sodium
HeLa Cells
Humans
Metal Nanoparticles
Nanocomposites
Plant Extracts
Rats
Silver
X-Ray Diffraction
C reactive protein
carboxymethylcellulose
interleukin 1
interleukin 2
interleukin 6
plant extract
silver nanoparticle
Syzygium aromaticum extract
unclassified drug
antiinfective agent
metal nanoparticle
nanocomposite
silver
animal cell
animal experiment
animal model
antileishmanial activity
Article
atomic force microscopy
bacterial kinetics
bacterial membrane
cell mediated cytotoxicity
chemical structure
clove
controlled study
cotton pellet-induced granuloma
female
HeLa cell line
IC50
in vitro study
in vivo study
LC50
Leishmania major
leukocyte count
male
nanofabrication
nonhuman
particle size
paw edema
pore size
rat
structure analysis
synthesis
X ray diffraction
zeta potential
animal
human
M3 - Article
PY - 2021
SP - 5371-5393
ST - Green synthesis and characterization of carboxymethyl cellulose fabricated
silver-based nanocomposite for various therapeutic applications
TI - Green synthesis and characterization of carboxymethyl cellulose fabricated
silver-based nanocomposite for various therapeutic applications
85113159323&doi=10.2147%2fIJN.S321419&partnerID=40&md5=f30c413bd60eb03167cf43e63bcc
83c0
VL - 16
ID - 5193
ER -
TY - JOUR
AB - Driven by the need to biosynthesized alternate biomedical agents to prevent
and treat infection, copper oxide nanoparticles (CuONPs) have surfaced as a
promising avenue. Cyanobacteria-derived synthesis of CuONPs is of substantive
interest as it offers an eco-friendly, cost-effective, and biocompatible route. In
the present study biosynthesized CuONPs were characterized and investigated
regarding their toxicity. Morphological analysis using TEM, SEM and AFM showed the
spherical particle size of 20.7 nm with 96% copper that confirmed the purity of
CuONPs. Biogenic CuONPs with IC50 value of 64.6 mu g ml(-1) showed 90% scavenging
of free radicals in superoxide radical scavenging assay. CuONPs showed enhanced
anti-inflammatory activity by 86% of protein denaturation with IC50 value of 89.9
mu g ml(-1). Biogenic CuONPs exhibited significant toxicity against bacterial
strains with lowest MIC value of 62.5 mu g ml(-1) for B. cereus and fungal strain
with a MIC value of 125 mu g ml(-1) for C. albicans. In addition CuONPs
demonstrated a high degree of synergistic interaction when combined with standard
drugs. CuONPs exhibited significant cytotoxicity against non-small cell lung cancer
with an IC50 value of 100.8 mu g ml(-1) for A549 and 88.3 mu g ml(-1) for the H1299
cell line with apoptotic activities. Furthermore, biogenic CuONPs was evaluated for
their photocatalytic degradation potential against methylene blue dye and were able
to removed 94% dye in 90 min. Free radical scavenging analysis suggested that
CuONPs assisted dye degradation was mainly induced by hydroxide radicals. Biogenic
CuONPs appears as an eco-friendly and cost effective photocatalyst for the
treatment of wastewater contaminated with synthetic dyes that poses threat to
aquatic biota and human health. The present study highlighted the blend of
biomedical and photocatalytic potential of Phormidium derived CuONPs as an
attractive approach for future applications in nanomedicine and bioremediation.
AN - WOS:000976817400030
AU - Asif, N.
AU - Ahmad, R.
AU - Fatima, S.
AU - Shehzadi, S.
AU - Siddiqui, T.
AU - Zaki, A.
AU - Fatma, T.
DA - APR 17
DO - 10.1038/s41598-023-33360-3
IS - 1
PY - 2023
SN - 2045-2322
ST - Toxicological assessment of Phormidium sp. derived copper oxide nanoparticles
for its biomedical and environmental applications
T2 - SCIENTIFIC REPORTS
TI - Toxicological assessment of Phormidium sp. derived copper oxide nanoparticles
for its biomedical and environmental applications
VL - 13
ID - 6593
ER -
TY - JOUR
AB - Peripheral neuropathies are common sequelae to human immunodeficiency virus
(HIV) infection in humans and are due to a variety of mechanisms, including direct
antiretroviral toxicity, HIV-mediated damage, immune-mediated disorders, and
opportunistic viral infections. Rhesus macaques (Macaca mulatta) infected with
simian immunodeficiency virus (SIV) remain the most consistent animal model for
unraveling the pathogenesis of lentiviral-associated disease and its associated
opportunistic infections. Rhesus cytomegalovirus (RhCMV) is the most common
opportunistic viral infection in rhesus macaques infected with SIV and causes
multiorgan pathology; however, its role in peripheral nerve pathology has not been
explored. We have identified 115 coinfected cases with SIV and RhCMV, of which 10
cases of RhCMV-associated facial neuritis were found (8.7% prevalence). Histologic
lesions were consistent in all cases and ranged from partial to complete
obliteration of the nerves of the tongue, lacrimal gland, and other facial tissues
with a mixed inflammatory population of neutrophils and macrophages, of which the
latter commonly contained intranuclear inclusion bodies. Luxol fast blue staining
and myelin basic protein immunohistochemistry confirmed the progressive myelin loss
in the peripheral nerves. Bielschowsky silver stain revealed progressive loss of
axons directly related to the severity of inflammation. Double immunohistochemistry
with spectral imaging analysis revealed RhCMV-infected macrophages directly
associated with the neuritis, and there was no evidence to support RhCMV infection
of Schwann cells. These results suggest that peripheral nerve damage is a bystander
effect secondary to inflammation rather than a direct infection of Schwann cells
and warrants further investigations into the pathogenesis of RhCMV-induced
peripheral neuropathy.
AN - WOS:000347300600029
AU - Assaf, B. T.
AU - Knight, H. L.
AU - Miller, A. D.
DA - JAN
DO - 10.1177/0300985814529313
IS - 1
PY - 2015
SN - 0300-9858
1544-2217
SP - 217-223
ST - Rhesus Cytomegalovirus (Macacine Herpesvirus 3)-Associated Facial Neuritis in
Simian Immunodeficiency Virus-Infected Rhesus Macaques (Macaca mulatta)
T2 - VETERINARY PATHOLOGY
TI - Rhesus Cytomegalovirus (Macacine Herpesvirus 3)-Associated Facial Neuritis in
Simian Immunodeficiency Virus-Infected Rhesus Macaques (Macaca mulatta)
VL - 52
ID - 6743
ER -
TY - JOUR
AB - Peripheral neuropathies are common sequelae to human immunodeficiency virus
(HIV) infection in humans and are due to a variety of mechanisms, including direct
antiretroviral toxicity, HIV-mediated damage, immune-mediated disorders, and
opportunistic viral infections. Rhesus macaques (Macaca mulatta) infected with
simian immunodeficiency virus (SIV) remain the most consistent animal model for
unraveling the pathogenesis of lentiviral-associated disease and its associated
opportunistic infections. Rhesus cytomegalovirus (RhCMV) is the most common
opportunistic viral infection in rhesus macaques infected with SIV and causes
multiorgan pathology; however, its role in peripheral nerve pathology has not been
explored. We have identified 115 coinfected cases with SIV and RhCMV, of which 10
cases of RhCMV-associated facial neuritis were found (8.7% prevalence). Histologic
lesions were consistent in all cases and ranged from partial to complete
obliteration of the nerves of the tongue, lacrimal gland, and other facial tissues
with a mixed inflammatory population of neutrophils and macrophages, of which the
latter commonly contained intranuclear inclusion bodies. Luxol fast blue staining
and myelin basic protein immunohistochemistry confirmed the progressive myelin loss
in the peripheral nerves. Bielschowsky silver stain revealed progressive loss of
axons directly related to the severity of inflammation. Double immunohistochemistry
with spectral imaging analysis revealed RhCMV-infected macrophages directly
associated with the neuritis, and there was no evidence to support RhCMV infection
of Schwann cells. These results suggest that peripheral nerve damage is a bystander
effect secondary to inflammation rather than a direct infection of Schwann cells
and warrants further investigations into the pathogenesis of RhCMV-induced
peripheral neuropathy. © The Author(s) 2014.
AU - Assaf, B. T.
AU - Knight, H. L.
AU - Miller, A. D.
DB - Scopus
DO - 10.1177/0300985814529313
IS - 1
KW - cytomegalovirus
facial nerve diseases
Macaca mulatta
macacine herpesvirus 3
neuritis
peripheral nervous system diseases
rhesus
Animals
Cytomegalovirus
Cytomegalovirus Infections
Facial Nerve Diseases
Opportunistic Infections
Peripheral Nervous System
Simian Acquired Immunodeficiency Syndrome
Simian Immunodeficiency Virus
animal
complication
cytomegalovirus infection
disease model
facial nerve disease
isolation and purification
opportunistic infection
pathology
peripheral nervous system
rhesus monkey
simian acquired immunodeficiency syndrome
Simian immunodeficiency virus
veterinary
virology
M3 - Article
PY - 2015
SP - 217-223
ST - Rhesus Cytomegalovirus (Macacine Herpesvirus 3)–Associated Facial Neuritis in
Simian Immunodeficiency Virus–Infected Rhesus Macaques (Macaca mulatta)
T2 - Veterinary Pathology
TI - Rhesus Cytomegalovirus (Macacine Herpesvirus 3)–Associated Facial Neuritis in
Simian Immunodeficiency Virus–Infected Rhesus Macaques (Macaca mulatta)
84920020117&doi=10.1177%2f0300985814529313&partnerID=40&md5=b7f90d51c7d427820c1262b
e85a22868
VL - 52
ID - 5611
ER -
TY - JOUR
AB - Metal oxide nanoparticles are widely used in both consumer products and
medical applications, but the knowledge regarding exposure-related health effects
is limited. However, it is challenging to investigate nanoparticle interaction
processes with biological systems. The overall aim of this project was to improve
the possibility to predict exposure-related health effects of metal oxide
nanoparticles through interdisciplinary collaboration by combining workflows from
the pharmaceutical industry, nanomaterial sciences, and occupational medicine.
Specific aims were to investigate nanoparticle-protein interactions and possible
adverse immune reactions. Four different metal oxide nanoparticles; CeOx
nanocrystals with 5% or 14% Gd, Co3O4, and Fe2O3, were characterized by dynamic
light scattering and high-resolution transmission electron microscopy.
Nanoparticle-binding proteins were identified and screened for HLA-binding peptides
in silico. Monocyte interaction with nanoparticle-protein complexes was assessed in
vitro. Herein, for the first time, immunogenic properties of nanoparticle-binding
proteins have been characterized. The present study indicates that especially
Co3O4-protein complexes can induce both 'danger signals', verified by the
production of inflammatory cytokines and simultaneously bind autologous proteins,
which can be presented as immunogenic epitopes by MHC class II. The clinical
relevance of these findings should be further evaluated to investigate the role of
metal oxide nanoparticles in the development of autoimmune disease. The general
workflow identified experimental difficulties, such as nanoparticle aggregate
formation and a lack of protein-free buffers suitable for particle
characterization, protein analyses, as well as for cell studies. This confirms the
importance of future interdisciplinary collaborations.
AN - WOS:000692910600001
AU - Assenhoj, M.
AU - Eriksson, P.
AU - Donnes, P.
AU - Ljunggren, S. A.
AU - Marcusson-Stahl, M.
AU - Du Rietz, A.
AU - Uvdal, K.
AU - Karlsson, H.
AU - Cederbrant, K.
C6 - AUG 2021
DA - SEP 14
DO - 10.1080/17435390.2021.1966115
IS - 8
PY - 2021
SN - 1743-5390
1743-5404
SP - 1035-1058
ST - Protein interaction, monocyte toxicity and immunogenic properties of cerium
oxide crystals with 5% or 14% gadolinium, cobalt oxide and iron oxide nanoparticles
- an interdisciplinary approach
T2 - NANOTOXICOLOGY
TI - Protein interaction, monocyte toxicity and immunogenic properties of cerium
oxide crystals with 5% or 14% gadolinium, cobalt oxide and iron oxide nanoparticles
- an interdisciplinary approach
VL - 15
ID - 6757
ER -
TY - JOUR
AB - Trichinellosis is a serious disease with no satisfactory treatment. We aimed
to assess the effect of myrrh (Commiphora molmol) and, for the first time, thyme
(Thymus vulgaris L.) against enteral and encysted (parenteral) phases of
Trichinella spiralis in mice compared with albendazole, and detect their effect on
inducible nitric oxide synthase (iNOS) expression. Oral administration of 500 mg/kg
of myrrh and thyme led to adult reduction (90.9%, 79.4%), while 1,000 mg/kg led to
larvae reduction (79.6%, 71.3%), respectively. Administration of 50 mg/kg of
albendazole resulted in adult and larvae reduction (94.2%, 90.9%). Positive
immunostaining of inflammatory cells infiltrating intestinal mucosa and submucosa
of all treated groups was detected. Myrrh-treated mice showed the highest iNOS
expression followed by albendazole, then thyme. On the other hand, both myrrh and
thyme-treated groups showed stronger iNOS expression of inflammatory cells
infiltrating and surrounding encapsulated T. spiralis larvae than albendazole
treated group. In conclusion, myrrh and thyme extracts are highly effective against
both phases of T. spiralis and showed strong iNOS expressions, especially myrrh
which could be a promising alternative drug. This experiment provides a basis for
further exploration of this plant by isolation and retesting the active principles
of both extracts against different stages of T. spiralis.
AD - Attia, Rasha AH
Assiut University. Faculty of Medicine. Department of Parasitology. Assiut. EG
Mahmoud, Abeer E
Farrag, Haiam Mohammed Mahmoud
Makboul, Rania
Mohamed, Mona Embarek
Ibraheim, Zedan
AU - Attia, Rasha A. H.
AU - Mahmoud, Abeer E.
AU - Farrag, Haiam Mohammed Mahmoud
AU - Makboul, Rania
AU - Mohamed, Mona Embarek
AU - Ibraheim, Zedan
C1 - 20160121
DA - 2015/12
DB - LILACS
DO - 10.1590/0074-02760150295
IS - 8
KW - Albendazole - thyme
Cytotoxicity tests
Enteral, parenteral phases
INOS
Limulus amoebocyte lysate assay
Myrrh
Trichinella spiralis
LA - en
PY - 2015
SN - 0074-0276
SP - 1035-1041
ST - Effect of myrrh and thyme on Trichinella spiralisenteral and parenteral
phases with inducible nitric oxide expression in mice
T2 - Mem. Inst. Oswaldo Cruz
TI - Effect of myrrh and thyme on Trichinella spiralisenteral and parenteral
phases with inducible nitric oxide expression in mice
UR - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-
02762015000801035
VL - 110
ID - 4934
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are of potential interest because of their
effective antibacterial and antiviral activities. Capping agents are used for
exhibiting a better antibacterial activity than uncapped Ag NPs. There are very few
reports that have shown the usage of AgNPs for in-vivo antibacterial therapy.
Citrate-capped silver nanoparticles were synthesized chemically by citrate
reduction method; the size of Cit-AgNPs was determined by an atomic force
microscope (AFM) and was between 15 - 90 nm. Acinetobacter baumannii (A. baumannii)
isolates were the only sensitive species to Cit-AgNPs. MICs and MBC of Cit-AgNPs
were determined by using A. baumannii. The results showed an additive effect of
Cit-AgNPs. Four mice groups were infected with a sub-lethal dose of A. baumannii
intraperitoneally, IP. The single daily dose of Cit-AgNPs and imipenem plus Cit-
AgNPs combination were administered IP. Imipenem and phosphate buffer saline (PBS)
was used as positive control and negative control, respectively. Interestingly,
only the PBS-treated group showed growth of A. baumannii in the liver and spleen of
sacrificed mice. Histopathologically, Cit-AgNPs showed antibacterial activity and
had an additive effect when combined with imipenem in vivo and in vitro. Moreover,
the Cit-AgNPs showed dose-dependent activity and the organs differed in the
illumination of the toxicity effect of Cit-AgNPs even after high dose
administration. In conclusion, Cit-capped AgNPs had antibacterial activity against
multiple drug resistant (MDR) A. baumannii but not against K. pneumoniae and E.
coli. Cit-capped AgNPs increased the inhibition zone of imipenem in additive
effect; the minimum inhibitory concentration and the minimum bactericidal
concentration of Cit-capped AgNPs were relatively low. Cit-capped AgNPs eliminated
A. baumannii infection in vivo when it was given alone or in combination with
imipenem. The cytotoxicity of Cit-AgNPs was dose-dependent and the organs differed
in the illumination of the inflammatory effect of Cit-AgNPs after high dose
administration. It is not recommended to use Cit-capped AgNPs systemically despite
their valuable additive antibacterial effect especially with a high dose and the
combination with imipenem, Topical administration needs to be evaluated. Copyright
© Ibtesam Ghadban Auda, Istabreq Mohamed Ali Salman, Dalal Abed Al-Sattar, and
Jameelah Ghadban Oduha. This is an open-access article distributed under the terms
of the Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original author and
source are credited.
AU - Auda, I. G.
AU - Salman, I. M. A.
AU - Al-Sattar, D. A.
AU - Oduha, J. G.
DB - Scopus
DO - 10.5101/nbe.v13i3.p229-239
IS - 3
KW - Acinetobacter baumannii
Citrate capped silver nanoparticles
In vitro
In vivo
RecA gene
amoxicillin plus clavulanic acid
cefotaxime
ceftazidime
ciprofloxacin
citric acid
gentamicin
imipenem
phosphate buffered saline
piperacillin plus tazobactam
silver nanoparticle
Acinetobacter infection
animal experiment
animal model
animal tissue
antibiotic sensitivity
Article
bacterial growth
bacterium isolate
combination drug therapy
controlled study
drug potentiation
drug synthesis
Escherichia coli
histopathology
illumination
in vitro study
in vivo study
liver
mouse
multidrug resistant Acinetobacter baumannii
nonhuman
single drug dose
spleen
zone of inhibition
M3 - Article
PY - 2021
SP - 229-239
ST - In-vivo and In-vitro anti-acinetobacter baumannii activity of citrate-capped
T2 - Nano Biomedicine and Engineering
TI - In-vivo and In-vitro anti-acinetobacter baumannii activity of citrate-capped
85115930017&doi=10.5101%2fnbe.v13i3.p229-
239&partnerID=40&md5=7b1e9146d8371b876fcca91d8a96f5ee
VL - 13
ID - 5200
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) are used in a variety of consumers goods. Their
toxicological impact is currently intensely studied, mostly upon acute exposure,
but their intracellular dissolution and fate is rather poorly documented. In this
study, murine primary macrophages were exposed to a single high but non-lethal dose
of Ag-NPs or to repeated, low doses of Ag-NPs. Cells were either collected
immediately after acute exposure or after 72 h of recovery in the NP-free exposure
medium. Ag intracellular content and distribution were analyzed by particle-induced
X-ray emission, transmission electron microscopy coupled to energy-dispersive
spectroscopy analysis and inductively coupled plasma mass spectrometry. In
parallel, macrophage functionality as well as inflammatory and thiol-responses were
assessed after Ag-NP exposure. We show that Ag accumulation in macrophages is
similar upon acute and repeated exposure to Ag-NPs, and that Ag is partly expelled
from cells during the 72 h recovery stage. However, acute exposure leads to a
strong response of macrophages, characterized by reduced mitochondrial membrane
potential, phagocytic capacity and nitric oxide (NO) production upon
lipopolysaccharide (LPS) stimulation. Under this condition, we also show an
increased release of proinflammatory cytokines as well as a decreased release of
anti-inflammatory cytokines. This response is reversible since these biomarkers
reach their basal level after the recovery phase; and is much less intense in
repeatedly exposed cells. These results suggest that repeated exposure of
macrophages to Ag-NPs, which is a more realistic exposure scenario than acute
exposure, leads to significant Ag intracellular accumulation but a much less
intense toxicological response. © 2015 Taylor and Francis.
AU - Aude-Garcia, C.
AU - Villiers, F.
AU - Collin-Faure, V.
AU - Pernet-Gallay, K.
AU - Jouneau, P. H.
AU - Sorieul, S.
AU - Mure, G.
AU - Gerdil, A.
AU - Herlin-Boime, N.
AU - Carrière, M.
AU - Rabilloud, T.
DB - Scopus
DO - 10.3109/17435390.2015.1104738
IS - 5
KW - Macrophage
nanoparticle
silver
speciation
toxicity
Animals
Cytokines
Lipopolysaccharides
Macrophages
Membrane Potential, Mitochondrial
Metal Nanoparticles
Mice
Phagocytosis
Silver
Spectrometry, X-Ray Emission
glutathione
interleukin 10
interleukin 6
lipopolysaccharide
nitric oxide
silver nanoparticle
thiol
tumor necrosis factor alpha
cytokine
metal nanoparticle
animal cell
Article
bone marrow derived macrophage
controlled study
cytokine release
cytotoxicity
energy dispersive spectroscopy
exposure
in vitro study
inflammation
mass spectrometry
mitochondrial membrane potential
mouse
nanotoxicology
nonhuman
particle induced x ray emission analysis
phagocytosis
physical chemistry
priority journal
scanning transmission electron microscopy
spectroscopy
X ray analysis
animal
chemistry
dose response
drug effects
immunology
macrophage
metabolism
spectrometry
ultrastructure
M3 - Article
PY - 2016
SP - 586-596
ST - Different in vitro exposure regimens of murine primary macrophages to silver
nanoparticles induce different fates of nanoparticles and different toxicological
and functional consequences
T2 - Nanotoxicology
TI - Different in vitro exposure regimens of murine primary macrophages to silver
84946595346&doi=10.3109%2f17435390.2015.1104738&partnerID=40&md5=077c46d962d324903f
4c992e9bd3aa00
VL - 10
ID - 5470
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) are used in a variety of consumers' goods.
Their toxicological impact is currently intensely studied, mostly upon acute
exposure, but their intracellular dissolution and fate is rather poorly documented.
In this study, murine primary macrophages were exposed to a single high but non-
lethal dose of Ag-NPs or to repeated, low doses of Ag-NPs. Cells were either
collected immediately after acute exposure or after 72 h of recovery in the NP-free
exposure medium. Ag intracellular content and distribution were analyzed by
particle-induced X-ray emission, transmission electron microscopy coupled to
energy-dispersive spectroscopy analysis and inductively coupled plasma mass
spectrometry. In parallel, macrophage functionality as well as inflammatory and
thiol-responses were assessed after Ag-NP exposure. We show that Ag accumulation in
macrophages is similar upon acute and repeated exposure to Ag-NPs, and that Ag is
partly expelled from cells during the 72 h recovery stage. However, acute exposure
leads to a strong response of macrophages, characterized by reduced mitochondrial
membrane potential, phagocytic capacity and nitric oxide (NO) production upon
lipopolysaccharide (LPS) stimulation. Under this condition, we also show an
increased release of proinflammatory cytokines as well as a decreased release of
anti-inflammatory cytokines. This response is reversible since these biomarkers
reach their basal level after the recovery phase; and is much less intense in
repeatedly exposed cells. These results suggest that repeated exposure of
macrophages to Ag-NPs, which is a more realistic exposure scenario than acute
exposure, leads to significant Ag intracellular accumulation but a much less
intense toxicological response.
AN - WOS:000372756300008
AU - Villiers, F.
AU - Pernet-Gallay, K.
AU - Jouneau, P. H.
AU - Sorieul, S.
AU - Mure, G.
AU - Gerdil, A.
AU - Carriere, M.
AU - Rabilloud, T.
DA - MAY 27
DO - 10.3109/17435390.2015.1104738
IS - 5
PY - 2016
SN - 1743-5390
1743-5404
SP - 586-596
ST - Different in vitro exposure regimens of murine primary macrophages to silver
T2 - NANOTOXICOLOGY
TI - Different in vitro exposure regimens of murine primary macrophages to silver
VL - 10
ID - 6057
ER -
TY - JOUR
AB - Nanoparticles (NPs), in particular noble metal nanoparticles, have been
incorporated into many therapeutic and biodiagnostic applications. While these
particles have many advantageous physical and optical properties, little is known
about their intrinsic intracellular effects in biological environments. Here, we
report the possible cell death mechanisms triggered in human oral squamous cell
carcinoma (HSC-3) cells after exposure to extracellular, cytoplasm, and nuclear
localized AuNPs and AgNPs. NP uptake and localization, cell viability, ATP levels,
modes of cell death, ROS generation, mitochondrial depolarization, and the levels
and/or translocation of caspase-dependent and caspase-independent proteins were
assessed under control and localized metal nanoparticle exposure. Exposure to AuNPs
resulted the adoption of a quiescent cellular state, as AuNPs caused a decrease in
intracellular ATP, but no change in viability or cell death populations. However,
AgNP exposure significantly reduced HSC-3 cell viability and increased apoptotic
populations, especially when localized at the cytoplasm and nucleus. Increased cell
death populations were linked to an increase in intracellular ROS generation.
Western blot analysis indicated cytoplasm localized AgNPs and nuclear localized
AgNPs utilized a caspase-independent apoptotic pathway that involved the nuclear
translocation of AIF and p38 MAPK proteins. These results demonstrate that the
degree of cytotoxicity increases as AgNPs move from extracellular localization to
nuclear localization, whereas changing AuNP localization does not trigger any
significant cytotoxicity. © 2014 Elsevier Ltd.
AU - Austin, L. A.
AU - Ahmad, S.
AU - Kang, B.
AU - Rommel, K. R.
AU - Mahmoud, M.
AU - Peek, M. E.
AU - El-Sayed, M. A.
DB - Scopus
DO - 10.1016/j.tiv.2014.11.003
IS - 4
KW - Apoptosis
Cytotoxicity
Gold nanoparticles
Organelle-targeted nanoparticles
Caspases
Cell Line, Tumor
Cell Nucleus
Cell Survival
Cytoplasm
Gold
Humans
Metal Nanoparticles
Necrosis
Particle Size
Peptides
Polyethylene Glycols
Silver
adenosine triphosphate
allograft inflammatory factor
caspase
cytokine
gold nanoparticle
mitogen activated protein kinase p38
silver nanoparticle
unclassified drug
gold
macrogol derivative
metal nanoparticle
peptide
silver
apoptosis
Article
cell death
cell nucleus
cell transport
cell viability
controlled study
cytoplasm
cytotoxicity
depolarization
dielectric constant
energy yield
enzyme inhibition
exposure
extracellular space
HSC 3 cell line
human
human cell
hydrodynamics
mitochondrial membrane
mitochondrion
mouth squamous cell carcinoma
necrosis
nuclear localization signal
oxidative stress
protein function
protein transport
Western blotting
zeta potential
cell survival
chemistry
drug effects
metabolism
particle size
tumor cell line
M3 - Article
PY - 2015
SP - 694-705
ST - Cytotoxic effects of cytoplasmic-targeted and nuclear-targeted gold and
silver nanoparticles in HSC-3 cells - A mechanistic study
T2 - Toxicology in Vitro
TI - Cytotoxic effects of cytoplasmic-targeted and nuclear-targeted gold and
silver nanoparticles in HSC-3 cells - A mechanistic study
84924308261&doi=10.1016%2fj.tiv.2014.11.003&partnerID=40&md5=e7b8047a82ffd2a9de5a9b
823b4e6d5a
VL - 29
ID - 5582
ER -
TY - JOUR
AB - Molds are filamentous fungi able to grow on a variety of surfaces, including
constructed surfaces, food, rotten organic matter, and humid places. Mold growth is
characterized by having an unpleasant odor in enclosed or non-ventilated places and
a non-aesthetic appearance. They represent a health concern because of their
ability to produce and release mycotoxins, compounds that are toxic to animals and
humans. The aim of this study was to evaluate commercial nanoparticles (NPs) that
can be used as an additive in coatings and paints to effectively control the growth
of harmful molds. Four different NPs were screened for their antifungal activities
against the mycotoxin producing mold strains Aspergillus flavus and A. fumigatus.
The minimal inhibitory concentrations of the NPs were determined in broth media,
whereas an agar diffusion test was used to assess the antimold activity on acrylic-
and water-based paints. The cytotoxic activity and the inflammatory response of the
NPs were also evaluated using the established human derived macrophage cell line
THP-1. Results showed that a combination of mix metallic- and ZnO-NPs (50:10 mu
g/mL) effectively inhibited the fungal growth when exposed to fluorescent light.
Neither cytotoxic effect nor inflammatory responses were recorded, suggesting that
this combination can be safely used in humid or non-ventilated environments without
any health concerns.
AN - WOS:000396167300058
AU - Auyeung, A.
AU - Casillas-Santana, M. A.
AU - Martinez-Castanon, G. A.
AU - Slavin, Y. N.
AU - Zhao, W. N.
AU - Asnis, J.
AU - Hafeli, U. O.
AU - Bach, H.
C7 - e0169940
DA - JAN 25
DO - 10.1371/journal.pone.0169940
IS - 1
PY - 2017
SN - 1932-6203
ST - Effective Control of Molds Using a Combination of Nanoparticles
T2 - PLOS ONE
TI - Effective Control of Molds Using a Combination of Nanoparticles
VL - 12
ID - 6804
ER -
TY - JOUR
AB - Molds are filamentous fungi able to grow on a variety of surfaces, including
constructed surfaces, food, rotten organic matter, and humid places. Mold growth is
characterized by having an unpleasant odor in enclosed or non-ventilated places and
a non-aesthetic appearance. They represent a health concern because of their
ability to produce and release mycotoxins, compounds that are toxic to animals and
humans. The aim of this study was to evaluate commercial nanoparticles (NPs) that
can be used as an additive in coatings and paints to effectively control the growth
of harmful molds. Four different NPs were screened for their antifungal activities
against the mycotoxin producing mold strains Aspergillus flavus and A. fumigatus.
The minimal inhibitory concentrations of the NPs were determined in broth media,
whereas an agar diffusion test was used to assess the antimold activity on acrylic-
and water-based paints. The cytotoxic activity and the inflammatory response of the
NPs were also evaluated using the established human derived macrophage cell line
THP-1. Results showed that a combination of mix metallic- and ZnO-NPs (50:10 μg/mL)
effectively inhibited the fungal growth when exposed to fluorescent light. Neither
cytotoxic effect nor inflammatory responses were recorded, suggesting that this
combination can be safely used in humid or non-ventilated environments without any
health concerns. © 2017 Auyeung et al.This is an open access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited.
AU - Auyeung, A.
AU - Casillas-Santana, M. Á
AU - Martínez-Castañón, G. A.
AU - Slavin, Y. N.
AU - Zhao, W.
AU - Asnis, J.
AU - Häfeli, U. O.
AU - Bach, H.
C7 - e0169940
DB - Scopus
DO - 10.1371/journal.pone.0169940
IS - 1
KW - Acrylates
Aspergillus flavus
Cell Line
Culture Media
Cytokines
Disk Diffusion Antimicrobial Tests
Fluorescence
Fungicides, Industrial
Gold
Humans
Inflammation
Macrophage Activation
Macrophages
Nanoparticles
Paint
Particle Size
Silver
Water
Zinc Oxide
gold nanoparticle
interleukin 6
metal nanoparticle
silver nanoparticle
acrylic acid
acrylic acid derivative
culture medium
cytokine
fungicide
gold
nanoparticle
paint
silver
water
zinc oxide
Article
controlled study
cytotoxicity
disk diffusion
fluorescent lighting
fungus growth
growth inhibition
human
human cell
inflammation
macrophage
nonhuman
cell line
chemically induced
comparative study
drug effects
fluorescence
immunology
macrophage activation
microbiology
particle size
radiation response
secretion (process)
M3 - Article
PY - 2017
ST - Effective control of molds using a combination of nanoparticles
T2 - PLoS ONE
TI - Effective control of molds using a combination of nanoparticles
85010960685&doi=10.1371%2fjournal.pone.0169940&partnerID=40&md5=ecba50cd47b91215bbb
40208921876f6
VL - 12
ID - 5495
ER -
TY - JOUR
AB - Nitrogen dioxide (NO2) is an environmental oxidant, known to be associated
with lung epithelial injury. In the present study, cellular pro-inflammatory
responses following exposure to a brief high concentration of NO2 (45 ppm) were
assessed, using normal human bronchial epithelial (NHBE) cells as an in vitro model
of inhalation injury. Generation and release of pro-inflammatory mediators such as
nitric oxide (NO), IL-8, TNF-alpha, IFN-gamma and IL-1beta were assessed at
different time intervals following NO2 exposure. Effects of a pre-existing
inflammatory condition was tested by treating the NHBE cells with different
inflammatory cytokines such as IFN-gamma, IL-8, TNF-alpha, IL-1beta, either alone
or in combination, before exposing them to NO2 . Immunofluorescence studies
confirmed oxidant-induced formation of 3-nitrotyrosine in the NO2-exposed cells. A
marked increase in the levels of nitrite (as an index of NO) and IL-8 were observed
in the NO2-exposed cells, which were further enhanced in the presence of the
cytokines. Effects of various NO inhibitors combined, with immunofluorescence and
Western blotting data, indicated partial contribution of the nitric oxide synthases
(NOSs) toward the observed increase in nitrite levels. Furthermore, a significant
increase in IL-1beta and TNF-alpha generation was observed in the NO2-exposed
cells. Although NO2 exposure alone did induce slight cytotoxicity (< 12%), but
presence of inflammatory cytokines such as TNF-alpha and IFN-gamma resulted in an
increased cell death (28-36%). These results suggest a synergistic role of,
inflammatory mediators, particularly of NO and IL-8, in NO2-mediated early cellular
changes. Our results also demonstrate an increased sensitivity of the cytokine-
treated NHBE cells toward NO2, which may have significant functional implications
in vivo. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
AN - WOS:000220322700007
AU - Ayyagari, V. N.
AU - Januszkiewicz, A.
AU - Nath, J.
DA - APR 15
DO - 10.1016/j.tox.2003.12.017
IS - 2
PY - 2004
SN - 0300-483X
SP - 149-164
ST - Pro-inflammatory responses of human bronchial epithelial cells to acute
nitrogen dioxide exposure
T2 - TOXICOLOGY
TI - Pro-inflammatory responses of human bronchial epithelial cells to acute
nitrogen dioxide exposure
VL - 197
ID - 6382
ER -
TY - JOUR
AB - Nitrogen Dioxide (NO2) is a product of high-temperature combustion and an
environmental oxidant of concern. We have recently reported that early changes in
NO2-exposed human bronchial epithelial cells are causally linked to increased
generation of proinflammatory mediators, such as nitric oxide/nitrite and cytokines
like interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha and IL-8. The
objective of the present in vitro study was to further delineate the cellular
mechanisms of NO2-mediated toxicity, and to define the nature of cell death that
ensues upon exposure of normal human bronchial epithelial (NHBE) cells to a brief
high dose of NO2. Our results demonstrate that the NHBE cells undergo apoptotic
cell death during the early post-NO2 period, but this is independent of any
significant increase in caspase-3 activity. However, necrotic cell death was more
prevalent at later time intervals. Interestingly, an increased expression of HO-1,
a redox-sensitive stress protein, was observed in NO2-exposed NHBE cells at 24 h.
Since neutrophils (PMNs) play an active role in acute lung inflammation and
resultant oxidative injury, we also investigated changes in human PMN-NHBE cell
interactions. As compared to normal cells, increased adhesion of PMNs to NO2-
exposed cells was observed, which resulted in an increased NHBE cell death. The
latter was also increased in the presence of IL-8 and TNF-alpha + interferon (IFN)-
gamma, which correlated with upregulation of intercellular adhesion molecule-1
(ICAM-1). Our results confirmed an involvement of nitric oxide (NO) in NO2-induced
cytotoxicity. By using NO synthase inhibitors such as L-NAME and 3-aminoguanidine
(AG), a significant decrease in cell death, PMN adhesion, and ICAM-1 expression was
observed. These findings indicate a role for the L-arginine/NO synthase pathway in
the observed NO2-mediated toxicity in NHBE cells. Therapeutic strategies aimed at
controlling excess generation of NO and/or inflammatory cytokines may be useful in
alleviating NO2-mediated adverse effects on the bronchial epithelium.
AN - WOS:000242852300008
AU - Ayyagari, V. N.
AU - Januszkiewicz, A.
AU - Nath, J.
DA - JAN 15
DO - 10.1080/08958370601052121
IS - 2
PY - 2007
SN - 0895-8378
1091-7691
SP - 181-194
ST - Effects of nitrogen dioxide on the expression of intercellular adhesion
molecule-1, neutrophil adhesion, and cytotoxicity: Studies in human bronchial
epithelial cells
T2 - INHALATION TOXICOLOGY
TI - Effects of nitrogen dioxide on the expression of intercellular adhesion
molecule-1, neutrophil adhesion, and cytotoxicity: Studies in human bronchial
epithelial cells
VL - 19
ID - 6390
ER -
TY - JOUR
AB - Objective(s): Diabetes is related with the higher blood levels of liver
enzymes and inflammatory factors. Galega officinalis is used as a medicinal plant
for treatment of diabetes traditionally. In this work, silver nanoparticles (Ag-
NPs) were synthesized with green method using Galega officinalis extract. Materials
and Methods: 'I he synthesized green Ag-N Ps were characterized completely. Intact
or diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg green
synthesized Ag-NPs. Mean serum levels of glucose, hepatic enzymes and hematological
parameter were determined. Gene expression of tumor necrotic factor alpha (TNF-
alpha) was done by real-time PCR. Results: Synthesis of green synthesized Ag-NPs
was confirmed by FT-IR, XRD and UV-vis analyses. The FESEM and TEM images showed
spherical Ag-NPs with size of 25 nm. The hypoglycemic influence of Ag-NPs using
Galega officinalis extract is reported for the first time in this study. Blood
concentration of liver enzymes, urea, glucose, white blood cells count and TNF-
alpha mRNA levels in visceral adipose tissue significantly declined in diabetic
rats receiving Ag-NPs. Conclusion: The synthesized Ag-NPs using Galega officinalis
extract may improve complication of diabetes via preventing liver hepatocyte damage
and reducing inflammatory factors.
AN - WOS:000702744800003
AU - Azimi, F.
AU - Mahmoudi, F.
AU - Amini, M. M.
DA - FAL
DO - 10.22038/NMJ.2021.59391.1613
IS - 4
PY - 2021
SN - 2322-3049
2322-5904
SP - 255-263
ST - Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic
effects in type 1 diabetic rats
T2 - NANOMEDICINE JOURNAL
TI - Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic
effects in type 1 diabetic rats
VL - 8
ID - 6126
ER -
TY - JOUR
AB - Azadirachta indica (Neem) is a large tree that is native to India and is
traditionally used due to its several properties, mainly to treat skin diseases, as
well as its "herbicidal" activity. Its bark, leaves, seeds, fruits and flowers are
widely used in medicinal treatment due to the presence of active secondary
metabolites with biological effects, mainly limonoids and tetranortriterpenoids,
such as azadirachtin. Thus, A. indica was studied in a variety of conditions, such
as anticancer, antiseptic, anti-inflammatory and chemopreventive agents, as well as
a biopesticide. Furthermore, differentiated cell tissue in A. indica cultivation
was reported to produce active metabolites for different purposes. However, only a
few studies have been developed regarding its potential use in cosmetics. For
instance, most studies explained the antimicrobial properties in health conditions,
such as acne, dandruff and personal health care. Here, we summarized not only the
most common cosmetic claims to treat acne but also mitigating other skin disorders
related to inflammatory and oxidant processes in recent in vivo studies and patents
to aid researchers and industrialists to select A. indica derivatives as novel
cosmetic ingredients.
AN - WOS:000816142400001
AU - Baby, A. R.
AU - Freire, T. B.
AU - Marques, G. D.
AU - Rijo, P.
AU - Lima, F. V.
AU - de Carvalho, J. C. M.
AU - Rojas, J.
AU - Magalhaes, W. V.
AU - Velasco, M. V. R.
AU - Morocho-Jacome, A. L.
C7 - 58
DA - JUN
DO - 10.3390/cosmetics9030058
IS - 3
PY - 2022
SN - 2079-9284
ST - Azadirachta indica (Neem) as a Potential Natural Active for Dermocosmetic and
Topical Products: A Narrative Review
T2 - COSMETICS
TI - Azadirachta indica (Neem) as a Potential Natural Active for Dermocosmetic and
Topical Products: A Narrative Review
VL - 9
ID - 6586
ER -
TY - JOUR
AB - Objective: The study evaluates the effect of adding graphene-Ag nanoparticles
(G-AgNp) to a PMMA auto-polymerizing resin, with focus on antibacterial activity,
cytotoxicity, monomer release, and mechanical properties. Materials and methods:
Auto-polymerizing acrylic resin (M) was loaded with 1 wt% G-AgNp (P1) and 2 wt% G-
AgNp (P2). Methyl methacrylate monomer release (MMA) was measured after immersion
of the samples in chloroform and cell medium respectively. Cell viability was
assessed on dysplastic oral keratinocytes (DOK) and dental pulp stem cells.
Oxidative stress and inflammatory response following exposure of dysplastic oral
keratinocytes to the experimental resins was evaluated. Antibacterial activity
against Staphylococcus aureus, Streptococcus mutans and Escherichia coli and also
flexural strength of the resins were assessed. Results: Residual monomer: For
samples immersed in chloroform, MMA concentration reached high levels, 10.27 μg/g
for sample P1; MMA increased at higher G-AgNp loading; 0.63 μg/g MMA was found in
medium for P1, and less for sample P2. Cell viability: Both cell lines displayed a
viability decrease, but remained above 75%, compared to controls, when exposed to
undiluted samples. Inflammation: proinflammatory molecule TNF-α decreased when DOK
cultures were exposed to G-AgNp samples. MDA levels indicated increased oxidative
stress damage in cells treated with PMMA, confirmed by the antioxidant mechanism
activation, while samples containing G-AgNp induced an antioxidant effect. All
tested samples showed antibacterial properties against Gram-positive bacteria.
Samples containing G-AgNp also exhibited bactericide action on E. coli. Mechanical
properties: both samples containing G-AgNp improved flexural strength compared to
the sample resin, measured through elastic strength parameters. Conclusions: PMMA
resin loaded with G-AgNp presents promising antibacterial activity associated with
minimal toxicity to human cells, in vitro, as well as improved flexural properties.
Clinical relevance: These encouraging results obtained in vitro support further in
vivo investigation, to thoroughly check whether the PMMA loaded with graphene-
silver nanoparticles constitute an improvement over current denture materials. ©
2019, Springer-Verlag GmbH Germany, part of Springer Nature.
AU - Bacali, C.
AU - Baldea, I.
AU - Moldovan, M.
AU - Carpa, R.
AU - Olteanu, D. E.
AU - Filip, G. A.
AU - Nastase, V.
AU - Lascu, L.
AU - Badea, M.
AU - Constantiniuc, M.
AU - Badea, F.
DB - Scopus
DO - 10.1007/s00784-019-03133-2
IS - 8
KW - Antibacterial action
Biocompatibility
Graphene
PMMA resin
Denture Bases
Dentures
Escherichia coli
Flexural Strength
Graphite
Humans
Materials Testing
Metal Nanoparticles
Polymethyl Methacrylate
Silver
antiinfective agent
graphite
metal nanoparticle
silver
denture
denture base
human
materials testing
M3 - Article
PY - 2020
SP - 2713-2725
ST - Flexural strength, biocompatibility, and antimicrobial activity of a
polymethyl methacrylate denture resin enhanced with graphene and silver
nanoparticles
T2 - Clinical Oral Investigations
TI - Flexural strength, biocompatibility, and antimicrobial activity of a
polymethyl methacrylate denture resin enhanced with graphene and silver
nanoparticles
85075184307&doi=10.1007%2fs00784-019-03133-
2&partnerID=40&md5=6e96f69099c0cdf76916d8dca7373f50
VL - 24
ID - 5302
ER -
TY - JOUR
AB - While inhalation represents one of the most likely routes of exposure, the
toxicity and response of nanoparticles at concentrations expected from such an
exposure are not well understood. Here we characterized the in vitro response of
human A549 adenocarcinomic alveolar epithelial cells following exposure to gold
(AuNP) and silver (AgNP) nanoparticles at levels approximating an occupational
exposure. Changes in neither oxidative stress nor cytotoxicity were significantly
affected by exposure to AgNPs and AuNPs, regardless of NP type (Ag vs. Au),
concentration, surface ligand (citrate or tannic acid), or size. An inflammatory
response was, however, observed in response to 20 nm AgNPs and 20 nm AuNPs, where
significant differences in the release of interleukin (IL)- 8 but not IL-6 were
observed. Additional data demonstrated that increased IL-8 secretion was strongly
dependent on both nanoparticle size and concentration. Overall these data suggest
that, while not acutely toxic, occupational exposure to AuNPs and AgNPs may trigger
a significant inflammatory response in alveolar epithelium. Moreover, the
differential responses in IL-8 and IL-6 secretion suggest that NPs may induce a
response pathway that is distinct from those commonly elicited by allergens and
pathogens. © Springer Science+Business Media Dordrecht 2012.
AU - Bachand, G. D.
AU - Allen, A.
AU - Bachand, M.
AU - Achyuthan, K. E.
AU - Seagrave, J. C.
AU - Brozik, S. M.
C7 - 1212
DB - Scopus
DO - 10.1007/s11051-012-1212-y
IS - 10
KW - Nanotoxicology
Occupational health
Particle toxicology
Toxicology
Cells
Industrial hygiene
Nanoparticles
citric acid
gold nanoparticle
interleukin 6
interleukin 8
silver nanoparticle
tannin
Alveolar epithelial cells
Differential response
Gold and silver nanoparticles
Occupational exposure
article
cancer cell culture
controlled study
cytokine production
cytokine release
cytotoxicity
human
human cell
in vitro study
inflammation
lung alveolus epithelium
occupational exposure
oxidative stress
particle size
priority journal
M3 - Article
PY - 2012
ST - Cytotoxicity and inflammation in human alveolar epithelial cells following
exposure to occupational levels of gold and silver nanoparticles
T2 - Journal of Nanoparticle Research
TI - Cytotoxicity and inflammation in human alveolar epithelial cells following
exposure to occupational levels of gold and silver nanoparticles
84866479851&doi=10.1007%2fs11051-012-1212-
y&partnerID=40&md5=3ba3f5c6762e290bcdf99ccbd18affd1
VL - 14
ID - 5680
ER -
TY - JOUR
AB - Increasing production and application of silver nanoparticles (Ag NPs) have
raised concerns on their possible adverse effects on human health. However, a
comprehensive understanding of their effects on biological systems, especially
immunomodulatory responses involving various immune cell types and biomolecules
(e.g., cytokines and chemokines), is still incomplete. In this study, a singlecell-
based, high-dimensional mass cytometry approach is used to investigate the
immunomodulatory responses of Ag NPs using human peripheral blood mononuclear cells
(hPBMCs) exposed to polyvinyl-pyrrolidone (PVP)-coated Ag NPs of different core
sizes (i.e., 10-, 20-, and 40-nm). Although there were no severe cytotoxic effects
observed,PVPAg10 andPVPAg20 were excessively found in monocytes and dendritic
cells, whilePVPAg40 displayed more affinity with B cells and natural killer cells,
thereby triggering the release of proinflammatory cytokines such as IL-2, IL-17A,
IL-17F, MIP1β, TNFα, and IFNγ. Our findings indicate that under the exposure
conditions tested in this study, Ag NPs only triggered the inflammatory responses
in a size-dependent manner rather than induce cytotoxicity in hPBMCs. Our study
provides an appropriate ex vivo model to better understand the human immune
responses against Ag NP at a single-cell level, which can contribute to the
development of targeted drug delivery, vaccine developments, and cancer
radiotherapy treatments. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Bae, J.
AU - Ha, M.
AU - Perumalsamy, H.
AU - Lee, Y.
AU - Song, J.
AU - Yoon, T. H.
C7 - 630
DB - Scopus
IS - 3
KW - immune systems
immunomodulatory responses
mass cytometry
single-cell analysis
gamma interferon
interleukin 17
interleukin 17F
interleukin 2
macrophage inflammatory protein 1beta
povidone
silver nanoparticle
Article
B lymphocyte
binding affinity
cancer radiotherapy
cell specificity
coating (procedure)
controlled study
cytokine release
cytotoxicity
dendritic cell
drug research
ex vivo study
human
human cell
immune response
immunomodulation
inflammation
molecularly targeted therapy
monocyte
natural killer cell
particle size
peripheral blood mononuclear cell
single cell analysis
M3 - Article
PY - 2022
ST - Mass Cytometry Exploration of Immunomodulatory Responses of Human Immune
Cells Exposed to Silver Nanoparticles
T2 - Pharmaceutics
TI - Mass Cytometry Exploration of Immunomodulatory Responses of Human Immune
Cells Exposed to Silver Nanoparticles
85126971963&doi=10.3390%2fpharmaceutics14030630&partnerID=40&md5=bc18374039b1d230bf
eadb630abcf334
VL - 14
ID - 5060
ER -
TY - JOUR
AB - Objectives: The aim of this study was to investigate the cellular effects of
newly developed calcium phosphate-based sealers (CAPSEAL I and II) using cultured
human periodontal ligament cells (HPDLCs) compared with epoxy resin sealer (AH26;
Dentsply, DeTrey, Konstanz, Germany), zinc oxide eugenol [ZOE] sealer (extended
working time [EWT]; Kerr Corporation, Orange, CA), and CPC sealer (Sankin apatite
sealer; Sankin-kogyo, Tokyo, Japan). Methods: Cell viability by -(4,5-
dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide assay, cell attachment by
scanning electron microscopy, osteoblastic differentiation and inflammatory
mediators by reverse-transcriptase polymerase chain reaction, Western blotting, and
alizarin red staining were evaluated. Results: The cytotoxicities of CAPSEAL I and
II were less than those of AH 26 and EWT after 1 and 14 days. Cells on CAPSEAL I
and II were spread better as compared with those on other sealers. Mineralization
after 14 days and the expression of osteoblastic differentiation markers such as
alkaline phosphate and osteonectin messenger RNA increased in CAPSEAL I- and II-
exposed HPDLCs after 1 and 3 days, whereas the production of inflammatory
mediators, including cyclooxygeanse-2, inducible nitric oxide synthetase, and
reactive oxygen species (ROS), were lower than in other sealers. Conclusions: These
results suggest that both CAPSEAL I and II show less cytotoxicity and inflammatory
mediators compared with other sealers and have the potential to promote bone
regeneration as root canal sealers. Copyright © 2010 American Association of
Endodontists.
AU - Bae, W. J.
AU - Chang, S. W.
AU - Lee, S. I.
AU - Kum, K. Y.
AU - Bae, K. S.
AU - Kim, E. C.
DB - Scopus
DO - 10.1016/j.joen.2010.06.022
IS - 10
KW - Attachments
CAPSEAL
cytotoxicity
human dental pulp cells
inflammatory mediator
odontoblastic differentiation
Bismuth
Bone Regeneration
Calcium Phosphates
Cell Adhesion
Cell Differentiation
Cells, Cultured
Cyclooxygenase 2
Epoxy Resins
Humans
Nitric Oxide Synthase Type II
Odontoblasts
Osteonectin
Periodontal Ligament
Root Canal Filling Materials
Silver
Titanium
Zinc Oxide-Eugenol Cement
bismuth
calcium phosphate
cyclooxygenase 2
epoxy resin
epoxy resin AH 26
epoxy resin AH-26
inducible nitric oxide synthase
osteonectin
root canal filling material
silver
titanium
zinc oxide eugenol
article
biosynthesis
bone regeneration
cell adhesion
cell culture
comparative study
cytology
drug effect
human
metabolism
odontoblast
periodontal ligament
M3 - Article
PY - 2010
SP - 1658-1663
ST - Human periodontal ligament cell response to a newly developed calcium
phosphate-based root canal sealer
T2 - Journal of Endodontics
TI - Human periodontal ligament cell response to a newly developed calcium
phosphate-based root canal sealer
77956950105&doi=10.1016%2fj.joen.2010.06.022&partnerID=40&md5=831eacfb862d25738df52
8f8e81f886a
VL - 36
ID - 5693
ER -
TY - JOUR
AB - Methemoglobin-forming drugs, such as sodium nitrite (NaNO2), may exacerbate
oxidative toxicity under certain chronic or acute hemolytic settings. In this
study, we evaluated markers of renal oxidative stress and injury in guinea pigs
exposed to extracellular hemoglobin (Hb) followed by NaNO2 at doses sufficient to
simulate clinically relevant acute methemoglobinemia. NaNO2 induced rapid and
extensive oxidation of plasma Hb in this model. This was accompanied by increased
renal expression of the oxidative response effectors nuclear factor erythroid 2-
derived-factor 2 (Nrf-2) and heme oxygenase-1 (HO-1), elevated non-heme iron
deposition, lipid peroxidation, interstitial inflammatory cell activation,
increased expression of tubular injury markers kidney injury-1 marker (KIM-1) and
liver-fatty acid binding protein (L-FABP), podocyte injury, and cell death.
Importantly, these indicators of renal oxidative stress and injury were minimal or
absent following infusion of Hb or NaNO2 alone. Together, these results suggest
that the exposure to NaNO2 in settings associated with increased extracellular Hb
may potentiate acute renal toxicity via processes that are independent of NaNO2
induced erythrocyte methemoglobinemia. Published by Elsevier Ireland Ltd.
AN - WOS:000357228200009
AU - Baek, J. H.
AU - Zhang, X. Y.
AU - Williams, M. C.
AU - Hicks, W.
AU - Buehler, P. W.
AU - D'Agnillo, F.
DA - JUL 3
DO - 10.1016/j.tox.2015.04.007
PY - 2015
SN - 0300-483X
SP - 89-99
ST - Sodium nitrite potentiates renal oxidative stress and injury in hemoglobin
exposed guinea pigs
T2 - TOXICOLOGY
TI - Sodium nitrite potentiates renal oxidative stress and injury in hemoglobin
exposed guinea pigs
VL - 333
ID - 6770
ER -
TY - CHAP
AB - Inorganic nanoparticles (NPs) either based on metal oxides (iron oxide,
cerium oxide, titanium dioxide, silicon dioxide, etc.) or metals (gold and silver)
have now wide applications. Consequently it increases the probability of unintended
exposure that could affect workers as well as the general population including
susceptible people. Inhalation, ingestion, and dermal contact are the main routes
of exposure. Before reaching the epithelial barrier lining the respiratory tract,
the digestive tract, or the skin, NPs get in contact with biological fluids and
become covered by molecules present in these fluids forming the so-called corona.
The fate and the effects of NPs may be different according to the corona
composition as the cell membrane does not interact directly with the NPs surface
but with the NP surrounded by its corona. Endocytosis has been shown to be an
important route of NPs uptake. However, the rate and mechanism of uptake seem to be
cell-type dependent, cell density-dependent and vary for NPs of different size,
charge, and other surface properties. Uptake is mostly an energy-dependent process,
dependent on NPs size, shape, and charge. There is also some evidence of NPs
exocytosis allowing NPs to cross epithelial barrier and enter systemic circulation.
Different in vitro models have been proposed showing potential of different NPs to
translocate. NPs biodistribution have been studied in different in vivo models
after intravenous injection, oral ingestion, intratracheal instillation, or
inhalation showing that smaller NPs can be better eliminated, but are also more
widespread in secondary organs. Inhalation studies underline that NPs mainly remain
at the site of exposure and only a low amount translocates. NPs health effects are
widely studied. Toxicological studies performed on animals by intratracheal
instillation have underlined that the most predominant effect of NPs is the
induction of lung inflammation characterized by the increase of immune cells,
frequently macrophages and neutrophils, in the bronchoalveolar lavage and the
increased release of pro-inflammatory mediators (cytokines and chemokines), and all
this effects are dependent on dose, size, surface reactivity, and NPs composition.
There is also evidence of some cardiovascular and neurologic effects of NPs. NPs
toxicity mainly results from their ability to induce an oxidative stress resulting
from the ability of NPs to directly or indirectly generate reactive oxygen species
(ROS). Some studies have shown the role of specific interactions between NPs and
proteins in cell activation or cell metabolism suggesting potential additional
pathways of toxicity independent of oxidative stress. A better knowledge about the
NPs properties involved in their toxicity is expected in order to propose NPs safe
by design. © 2013 Springer-Verlag London. All rights are reserved.
AU - Baeza-Squiban, A.
AU - Vranic, S.
AU - Boland, S.
DB - Scopus
DO - 10.1007/978-1-4471-4213-3_9
KW - Cells
Iron oxides
Metabolism
Metal nanoparticles
Molecular biology
Nanoparticles
Oxides
Silver
Titanium oxides
Toxicity
Energy-dependent process
Inflammatory mediators
Inorganic nanoparticle
Intratracheal instillation
Intravenous injections
Manufactured nanoparticles
Specific interaction
Cytology
PY - 2013
SP - 245-267
ST - Fate and health impact of inorganic manufactured nanoparticles
T2 - Nanomaterials: A Danger or a Promise? A Chemical and Biological Perspective
TI - Fate and health impact of inorganic manufactured nanoparticles
84928925806&doi=10.1007%2f978-1-4471-4213-
3_9&partnerID=40&md5=02708a6a4ac7a6bfd3371215d1c241e9
VL - 9781447142133
ID - 5615
ER -
TY - JOUR
AB - In addition to excellent biocompatibility and mechanical performance, the new
generation of bone and craniofacial implants are expected to proactively contribute
to the regeneration process and dynamically interact with the host tissue. To this
end, integration and sustained delivery of therapeutic agents has become a rapidly
expanding area. The incorporated active molecules can offer supplementary features
including promoting oteoconduction and angiogenesis, impeding bacterial infection
and modulating host body reaction. Major limitations of the current practices
consist of low drug stability overtime, poor control of release profile and
kinetics as well as complexity of finding clinically appropriate drug dosage. In
consideration of the multifaceted cascade of bone regeneration process, this
research is moving towards dual/multiple drug delivery, where precise control on
simultaneous or sequential delivery, considering the possible synergetic
interaction of the incorporated bioactive factors is of utmost importance. Herein,
recent advancements in fabrication of synthetic load bearing implants equipped with
various drug delivery systems are reviewed. Smart drug delivery solutions, newly
developed to provide higher tempo-spatial control on the delivery of the
pharmaceutical agents for targeted and stimuli responsive delivery are highlighted.
The future trend of implants with bone drug delivery mechanisms and the most common
challenges hindering commercialization and the bench to bedside progress of the
developed technologies are covered. " (C) 2016 Elsevier B.V. All rights reserved.
AN - WOS:000390967200141
AU - Bagherifard, S.
DA - FEB 1
DO - 10.1016/j.msec.2016.11.011
PY - 2017
SN - 0928-4931
1873-0191
SP - 1241-1252
ST - Mediating bone regeneration by means of drug eluting implants: From passive
to smart strategies
T2 - MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
TI - Mediating bone regeneration by means of drug eluting implants: From passive
to smart strategies
VL - 71
ID - 6826
ER -
TY - JOUR
AB - The present investigation aims to explore therapeutic potential of biogenic
synthesised silver nanoparticles (AgNPs) mediated using endophytic fungus extract
isolated from Tinospora cordifolia. The synthesised AgNPs characterised using
various physicochemical analyses. In FTIR, amide I and II bands of protein and
hydroxyl groups of phenolic hydroxyl confirming bioactive metabolites presence over
AgNPs surface. The mean size of AgNPs found 25–35 nm spherical in size. Further,
AgNPs demonstrated higher antibacterial, antioxidant, and anti-inflammatory
activity than endophyte fungal extract. Such high activity of AgNPs might be a
resultant of the propounding synergistic activity of silver nanoparticle and
endophyte bioactive metabolites over AgNPs surface that is confirmed by FTIR.
Moreover, antiproliferative activity in cervical and breast cancer cells was
demonstrated. Finally, AgNPs found cytocompatibility and hemocompatibility. Thus,
the results obtained provide apparent and multiple biological activities of
biogenic silver nanoparticles synthesised as possible multipurpose therapeutic
material with little systemic toxicity. © 2020 Informa UK Limited, trading as
AU - Bagur, H.
AU - Medidi, R. S.
AU - Somu, P.
AU - Choudhury, P. W. J.
AU - karua, C. S.
AU - Guttula, P. K.
AU - Melappa, G.
AU - Poojari, C. C.
DB - Scopus
DO - 10.1080/10667857.2020.1819089
IS - 3
KW - anti-inflammatory activity
Biogenic synthesis
multipurpose therapeutic agent
synergistic effect
Amides
Metabolites
Metal nanoparticles
Anti-proliferative activities
Physico-chemical analysis
Synergistic activity
Therapeutic materials
Therapeutic potentials
M3 - Article
PY - 2022
SP - 167-178
ST - Endophyte fungal isolate mediated biogenic synthesis and evaluation of
biomedical applications of silver nanoparticles
T2 - Materials Technology
TI - Endophyte fungal isolate mediated biogenic synthesis and evaluation of
biomedical applications of silver nanoparticles
85091098023&doi=10.1080%2f10667857.2020.1819089&partnerID=40&md5=0c585f3a186c32918d
64757b1bbc59d5
VL - 37
ID - 5150
ER -
TY - JOUR
AB - In the present work, AgNPs have been prepared using an extract of Exserohilum
rostrata, an endophyte fungus isolated from Ocimum tenuiflorum leaf and
characterized using TEM, SEM, DLS, XRD, FT-IR, etc. The FT-IR analysis confirmed
the capping of AgNPs with bioactive molecules of endophyte extract, thereby
importing addition or enhancing inherent AgNPs therapeutic properties.
Both endophyte extract and AgNPs offered significant inhibition in Gram-positive
and Gram-negative bacteria. Further, we also demonstrated its ability to inhibit
bacterial biofilm formation of P. aeruginosa and S. aureus. Furthermore, we also
demontrated excellent antioxidant and anti-inflammatory activity of both endophyte
extract and AgNPs. Moreover, we observed ehanced antibacterial, anti-inflammatory,
and antioxidant activity of AgNPs which is due to the synergistic effect of the
bioactive agent forming the corona of AgNPs and with inhabitant activity of AgNPs.
We also demonstrated the antimitotic activity in A. cepa and antiproliferative
activity in breast cancer cells. AgNPs have also found to be excellent
compatibility with healthy human keratinocyte cells and RBC cells. Hence, we might
say that biogenically synthesized AgNPs using endophyte extract possess anti-
bacterial, anti-inflammatory, and anti-oxidant ability as well as antiproliferative
activity in breast cancer cells and thus possible found its application in the
biomedical industry due to it’s eco-friendly and cost-effectiveness. © 2019,
Springer Science+Business Media, LLC, part of Springer Nature.
AU - Bagur, H.
AU - Poojari, C. C.
AU - Melappa, G.
AU - Rangappa, R.
AU - Chandrasekhar, N.
AU - Somu, P.
DB - Scopus
DO - 10.1007/s10876-019-01731-4
IS - 6
KW - Anti-oxidant activity
Biocompatibility
Endophyte fungi
Green synthesis
M3 - Article
PY - 2020
SP - 1241-1255
ST - Biogenically Synthesized Silver Nanoparticles Using Endophyte Fungal Extract
of Ocimum tenuiflorum and Evaluation of Biomedical Properties
T2 - Journal of Cluster Science
TI - Biogenically Synthesized Silver Nanoparticles Using Endophyte Fungal Extract
of Ocimum tenuiflorum and Evaluation of Biomedical Properties
85075444921&doi=10.1007%2fs10876-019-01731-
4&partnerID=40&md5=4a83a42f2c82c96c7da462544ce04001
VL - 31
ID - 5355
ER -
TY - JOUR
AB - In the present work, AgNPs have been prepared using an extract ofExserohilum
rostrata, an endophyte fungus isolated fromOcimum tenuiflorumleaf and characterized
using TEM, SEM, DLS, XRD, FT-IR, etc. The FT-IR analysis confirmed the capping of
AgNPs with bioactive molecules of endophyte extract, thereby importing addition or
enhancing inherent AgNPs therapeutic properties. Both endophyte extract and AgNPs
offered significant inhibition in Gram-positive and Gram-negative bacteria.
Further, we also demonstrated its ability to inhibit bacterial biofilm formation
ofP. aeruginosaandS. aureus. Furthermore, we also demontrated excellent antioxidant
and anti-inflammatory activity of both endophyte extract and AgNPs. Moreover, we
observed ehanced antibacterial, anti-inflammatory, and antioxidant activity of
AgNPs which is due to the synergistic effect of the bioactive agent forming the
corona of AgNPs and with inhabitant activity of AgNPs. We also demonstrated the
antimitotic activity inA. cepaand antiproliferative activity in breast cancer
cells. AgNPs have also found to be excellent compatibility with healthy human
keratinocyte cells and RBC cells. Hence, we might say that biogenically synthesized
AgNPs using endophyte extract possess anti-bacterial, anti-inflammatory, and anti-
oxidant ability as well as antiproliferative activity in breast cancer cells and
thus possible found its application in the biomedical industry due to it's eco-
friendly and cost-effectiveness.
AN - WOS:000575451100002
AU - Bagur, H.
AU - Poojari, C. C.
AU - Melappa, G.
AU - Rangappa, R.
AU - Chandrasekhar, N.
AU - Somu, P.
C6 - NOV 2019
DA - NOV
DO - 10.1007/s10876-019-01731-4
IS - 6
PY - 2020
SN - 1040-7278
1572-8862
SP - 1241-1255
ST - Biogenically Synthesized Silver Nanoparticles Using Endophyte Fungal Extract
ofOcimum tenuiflorumand Evaluation of Biomedical Properties
T2 - JOURNAL OF CLUSTER SCIENCE
TI - Biogenically Synthesized Silver Nanoparticles Using Endophyte Fungal Extract
ofOcimum tenuiflorumand Evaluation of Biomedical Properties
VL - 31
ID - 5999
ER -
TY - JOUR
AB - Nanotechnology is a rapidly growing field having potential applications in
many areas. Nanoparticles (NPs) have been studied for cell toxicity,
immunotoxicity, and genotoxicity. Tetrazolium-based assays such as MTT, MTS, and
WST-1 are used to determine cell viability. Cell inflammatory response induced by
NPs is checked by measuring inflammatory biomarkers, such as IL-8, IL-6, and tumor
necrosis factor, using ELISA. Lactate dehydrogenase (LDH) assay is used for cell
membrane integrity. Different types of cell cultures, including cancer cell lines
have been employed as in vitro toxicity models. It has been generally agreed that
NPs interfere with either assay materials or with detection systems. So far,
toxicity data generated by employing such models are conflicting and inconsistent.
Therefore, on the basis of available experimental models, it may be difficult to
judge and list some of the more valuable NPs as more toxic to biological systems
and vice versa. Considering the potential applications of NPs in many fields and
the growing apprehensions of FDA about the toxic potential of nanoproducts, it is
the need of the hour to look for new internationally agreed free of bias
toxicological models by focusing more on in vivo studies. © 2016, Pasteur Institute
of Iran. All rights reserved.
AU - Bahadar, H.
AU - Maqbool, F.
AU - Niaz, K.
AU - Abdollahi, M.
DB - Scopus
IS - 1
KW - Cytotoxicity
In vitro
Metal nanoparticles
Review
Toxicology
Animals
Cell Line
Cell Survival
DNA Damage
Humans
Metal Nanoparticles
Nanostructures
Nanotechnology
aluminum oxide nanoparticle
carbon nanoparticle
gold nanoparticle
nanoparticle
silica nanoparticle
silver nanoparticle
ultrasmall superparamagnetic iron oxide
unclassified drug
metal nanoparticle
nanomaterial
apoptosis
blood brain barrier
cell structure
cell viability
chromosome damage
cytotoxicity
disorders of mitochondrial functions
DNA adduct
DNA damage
DNA strand breakage
genotoxicity
human
lipid peroxidation
nanotechnology
nanotoxicology
nonhuman
oxidative stress
protein expression
animal
cell line
cell survival
drug effects
physiology
procedures
trends
M3 - Review
PY - 2016
SP - 1-11
ST - Toxicity of nanoparticles and an overview of current experimental models
T2 - Iranian Biomedical Journal
TI - Toxicity of nanoparticles and an overview of current experimental models
84944755075&partnerID=40&md5=1c45a24b1c3af6c0ff01b0dcb9e7ed81
VL - 20
ID - 5614
ER -
TY - JOUR
AB - Background and Aims: Free-radical-mediated peroxidation of membrane lipids
and oxidative damage of DNA and proteins are believed to be associated with a
variety of chronic pathological complications such as cancer. The aim of this study
was to describe antioxidant and anti-inflammatory of silver nanoparticles (AgNPs)
synthesized using medicinal plant extract of Sage (Salvia officinalis). Materials
and Methods: AgNPs were synthesized using S. officinalis as reducer agents and
characterized using ultraviolet-visible, Fourier transform infrared spectroscopy,
particle seizer, and transmission electron microscopy. Toxicity of AgNPs on MCF-7
cells was investigated using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-
tetrazolium bromide (MTT) assay. Changes in expression of inflammation related
genes include cyclooxygenase-2, IL-8, and tumor necrosis factor-alpha (TNF-α) genes
were evaluated using semi-quantification reverse transcription-polymerase chain
reaction (RT-PCR). The antioxidant potential of capped AgNPs was assessed using 1,1
diphenyl-2-picryl-hydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-
sulfonic acid (ABTS) radicals activity assay. Results: AgNPs successfully synthesis
with an average size of 16 nm and spherical. FTIR spectrum from plant extract and
AgNPs indicated the extract covered nanoparticles. AgNPs decreased cells viability
with inhibitory concentration (IC50) of 25 μg/ml and 20 μg/ml after 24 and 48 h,
respectively. To ascertain the anti-inflammatory genes expression, MCF-7 cells were
treated with 20 μg/ml AgNPs (concentration below of IC50 value according to MTT
assay). Semi-quantification RT-PCR results showed that AgNPs increased IL-8 and
TNF-α genes expression 28.76% and 42%, respectively, but suppressed cyclooxygenase-
2 gene expression with 20.5% comparing to control groups. Antioxidant assay of
green synthesized AgNPs coated by S. officinalis extract showed free radical
scavenging effect with IC50 of 830 and 800 μg/ml for DPPH and ABTS radicals,
respectively. Conclusion: The coated AgNPs with S. officinalis have promising
potential as a source for the development of chemotherapeutic agents in future. ©
2017 Annals of Tropical Medicine and Public Health | Published by Wolters Kluwer -
Medknow.
AU - Baharara, J.
AU - Ramezani, T.
AU - Mousavi, M.
AU - Asadi-Samani, M.
DB - Scopus
DO - 10.4103/ATMPH.ATMPH_174_17
IS - 5
Antioxidant
Salvia officinalis
butylated hydroxyanisole
cyclooxygenase 2
glyceraldehyde 3 phosphate dehydrogenase
interleukin 8
Salvia officinalis extract
silver nanoparticle
ABTS radical scavenging assay
antioxidant assay
Article
cell survival
cell viability
comparative study
concentration response
controlled study
cytotoxicity
drug synthesis
gene expression
green chemistry
human
human cell
IC50
inflammation
nanotoxicology
particle size
reverse transcription polymerase chain reaction
M3 - Article
PY - 2017
SP - 1265-1270
ST - Antioxidant and anti-inflammatory activity of green synthesized silver
nanoparticles using Salvia officinalis extract
T2 - Annals of Tropical Medicine and Public Health
TI - Antioxidant and anti-inflammatory activity of green synthesized silver
nanoparticles using Salvia officinalis extract
85033221553&doi=10.4103%2fATMPH.ATMPH_174_17&partnerID=40&md5=54a8d2a6b64838da16b8b
42533081c1a
VL - 10
ID - 5512
ER -
TY - CONF
AB - The phytochemicals can act as reducing agents as well as nontoxic capping
materials of metal nanosilver. The objective of the present work was to fabricate
the synthesis of silver nanoparticle using aqueous bark extract of Saraca asoca as
reducing agent and testing the wound healing efficacy of the material by studying
the level of wound contraction and re-epithelialization and expression of
inflammatory factors like cytokines and different proinflammatory markers in
diabetic mice. The biocompatibility of the synthesized silver nanoparticle was
tested by cell cycle analysis, ROS generation, DAPI, comet assay. It was indicated
that the synthesized silver nanoparticles were nontoxic to normal mice in vivo.
Antibacterial properties of the silver formulation are comparable to different
antibiotics and more effective against Bacillus firmus and Staphylococcus
gallinarum of diabetic wound. SEM images and histopathological observations
revealed that the healing process was accelerated in the silver nanoparticle
treated group. The significant healing efficacy of phytofabricated nanoparticles
was evident by the observations of downregulation of proinflammatory cytokines such
as IL-1β, IL-6, and TNF-α, and upregulation of anti-inflammatory cytokine, IL-10. ©
2021, Springer Nature Singapore Pte Ltd.
AU - Bairagi, B.
AU - Nath, D.
DB - Scopus
DO - 10.1007/978-981-15-7409-2_48
KW - Antibacterial efficacy
Diabetic wound
Saraca asoca
Wound healing
PY - 2021
SP - 475-484
ST - Wound Healing and Antimicrobial Property of Phytofabricated Silver
Nanoparticle by Saraca asoca Bark Extract on Diabetic Wound In Mice
T2 - Lecture Notes in Bioengineering
TI - Wound Healing and Antimicrobial Property of Phytofabricated Silver
Nanoparticle by Saraca asoca Bark Extract on Diabetic Wound In Mice
85092898345&doi=10.1007%2f978-981-15-7409-
2_48&partnerID=40&md5=7d3172e549fd90aa540e83100bada97f
ID - 5327
ER -
TY - JOUR
AB - Fungal metabolites, proteins, and enzymes have been rich sources of
therapeutics so far. Therefore, in this study, the hypha extract of a newly
identified noble fungus (Alternaria sp. with NCBI Accession number: MT982648) was
used to synthesize silver nanoparticles (F-AgNPs) to utilize against bacteria,
fungi, and lung cancer. F-AgNPs were characterized by using physical techniques,
including UV–visible spectroscopy, zeta potential, DLS, XRD, TEM, and HR-TEM. The
particles were found to be polydispersed and quasi-spherical in shape under TEM.
They had an average size of ~15 nm. The well dispersed particles were found to have
consistent crystallinity with cubic phase geometry under XRD and HR-TEM. The
presence of different functional groups on the surfaces of biosynthesized F-AgNPs
was confirmed by FTIR. The particle distribution index was found to be 0.447 with a
hydrodynamic diameter of ~47 d.nm, and the high value of zeta potential (−20.3 mV)
revealed the stability of the nanoemulsion. These particles were found to be active
against Staphylococcus aureus (multidrug resistance-MDR), Klebsiella pneumonia,
Salmonella abony, and Escherichia coli (MDR) with MIC50 10.3, 12.5, 22.69, and
16.25 µg/mL, respectively. Particles also showed inhibition against fungal strains,
including A. flavus, A. niger, T. viridens, and F. oxysporium. Their inhibition of
biofilm formation by the same panel of bacteria was also found to be very promising
and ranged from 16.66 to 64.81%. F-AgNPs also showed anticancer potential (IC50—
21.6 µg/mL) with respect to methotrexate (IC50—17.7 µg/mL) against lung cancer cell
line A549, and they did not result in any significant inhibition of the normal cell
line BEAS-2. The particles were found to alter the mitochondrial membrane
potential, thereby disturbing ATP synthesis and leading to high ROS formation,
which are responsible for cell membrane damage and release of LDH, intracellular
proteins, lipids, and DNA. A high level of ROS also elicits pro-inflammatory
signaling cascades that lead to programmed cell death by either apoptosis or
necrosis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Baker, A.
AU - Iram, S.
AU - Syed, A.
AU - Elgorban, A. M.
AU - Al-Falih, A. M.
AU - Bahkali, A. H.
AU - Khan, M. S.
AU - Kim, J.
C7 - 3227
DB - Scopus
DO - 10.3390/nano11123227
IS - 12
KW - AgNPS
Antibacterial
Antibiofilm
Anticancer
Fungus
M3 - Article
PY - 2021
ST - Potentially bioactive fungus mediated silver nanoparticles
T2 - Nanomaterials
TI - Potentially bioactive fungus mediated silver nanoparticles
85120002675&doi=10.3390%2fnano11123227&partnerID=40&md5=17ef61d357ad7a40a490118a470
f3d10
VL - 11
ID - 5240
ER -
TY - JOUR
AB - The use of metal nanoparticles as supplements of animal diets does not always
bring unambiguous results. There are many reports in the literature about the
multifaceted effects of this type of supplementation on the animal organism.
Therefore, the aim of the paper is to present the current knowledge of the possible
application of nanometal forms in animal nutrition and its potential benefits and
threats. The positive effect of nanoparticles used as feed additives has most
frequently been reflected in an increase in body weight, higher average daily gain,
or improvement of the FCR value. In some cases, however, the effect of nanoparticle
addition to diets was indiscernible. The potent antibacterial activity of
nanoparticles, especially against Gram-negative bacteria and Gram-positive
bacteria, is regarded as a positive effect. In turn, the probability of their
toxicity is a potential risk in application thereof. Supplementation of diets with
nanometals has been accompanied by pathological changes in animal tissues,
primarily in the pancreas, kidney, liver, rumen, abomasum, small intestine, adrenal
glands, and brain. Additionally, at the the cellular level, nanoparticles were
found to induce toxicity, inflammatory excitation, and cell death. Oral
administration of nanoparticles induced a risk of malfunction of the nervous system
and even impairment of cognitive processes in animals. The increasing knowledge of
the possible toxic effects of nanoparticles on the animal organism suggests caution
in their use in animal production and necessitates further precise investigations
in this area.
AN - WOS:000449719600002
AU - Bakowski, M.
AU - Kiczorowska, B.
AU - Samolinska, W.
AU - Klebaniuk, R.
AU - Lipiec, A.
DA - OCT
DO - 10.2478/aoas-2018-0029
IS - 4
PY - 2018
SN - 2300-8733
SP - 879-898
ST - SILVER AND ZINC NANOPARTICLES IN ANIMAL NUTRITION - A REVIEW
T2 - ANNALS OF ANIMAL SCIENCE
TI - SILVER AND ZINC NANOPARTICLES IN ANIMAL NUTRITION - A REVIEW
VL - 18
ID - 6410
ER -
TY - JOUR
AB - The use of metal nanoparticles as supplements of animal diets does not always
bring unambiguous results. There are many reports in the literature about the
multifaceted effects of this type of supplementation on the animal organism.
Therefore, the aim of the paper is to present the current knowledge of the possible
application of nanometal forms in animal nutrition and its potential benefits and
threats. The positive effect of nanoparticles used as feed additives has most
frequently been reflected in an increase in body weight, higher average daily gain,
or improvement of the FCR value. In some cases, however, the effect of nanoparticle
addition to diets was indiscernible. The potent antibacterial activity of
nanoparticles, especially against Gram-negative bacteria and Gram-positive
bacteria, is regarded as a positive effect. In turn, the probability of their
toxicity is a potential risk in application thereof. Supplementation of diets with
nanometals has been accompanied by pathological changes in animal tissues,
primarily in the pancreas, kidney, liver, rumen, abomasum, small intestine, adrenal
glands, and brain. Additionally, at the the cellular level, nanoparticles were
found to induce toxicity, inflammatory excitation, and cell death. Oral
administration of nanoparticles induced a risk of malfunction of the nervous system
and even impairment of cognitive processes in animals. The increasing knowledge of
the possible toxic effects of nanoparticles on the animal organism suggests caution
in their use in animal production and necessitates further precise investigations
in this area. © 2018 Maciej BAkowski et al., published by Sciendo 2018.
AU - Bakowski, M.
AU - Kiczorowska, B.
AU - Samolińska, W.
AU - Klebaniuk, R.
AU - Lipiec, A.
DB - Scopus
DO - 10.2478/aoas-2018-0029
IS - 4
KW - animal production
bactericidal properties
metal nanoparticles
nutrition
toxicity
M3 - Review
PY - 2018
SP - 879-898
ST - Silver and Zinc Nanoparticles in Animal Nutrition-A Review
T2 - Annals of Animal Science
TI - Silver and Zinc Nanoparticles in Animal Nutrition-A Review
85113166830&doi=10.2478%2faoas-2018-
0029&partnerID=40&md5=f017308c29cf82d259fab6ca3124d8a9
VL - 18
ID - 5367
ER -
TY - JOUR
AB - Biosynthesized low dimensional (<10 nm) nanoparticles are gaining great
attention in biomedical applications. Present research emphasizes a comparative
study of silver nanoparticles (Ag NPs) and Ag NPs decorated with reduced graphene
oxide nanocomposites (Ag NPs@rGO) synthesized from chemical and green methods, and
appraisal of their bioactivities and toxicity. We have used a unique sida acuta
leaf extract containing a multiplex combination of assorted bioactive compounds, as
a reducing agent of NPs formation from metal ions. We controlled the synthesis
conditions of molar ratio, pH, and reaction time to obtain performance enhanced Ag
NPs. Extremely-tiny spherical shaped Ag NPs (5 nm) with quite a delicate particle
dispersion over mono-layer graphene nanosheets was observed in green mediated Ag
NPs@rGO.The green synthesized Ag NPs@rGO outperformed to chemically prepared Ag NPs
and their composites for 500 μg/mL opposed to Methicillin-resistant Staphylococcus
aureus (MRSA) and Proteus mirabili bacteria. The G-Ag NPs and G-Ag NPs@rGO
demonstrated the highest cytotoxicity rate against MCF-7 cells at the half-maximal
inhibitory concentration (IC50) of 100 μg/mL and 108 μg/mL. Moreover, the green
mediated Ag NPs showed 99.18% anti-inflammatory, 96.09% anti-diabetic and 75.68%
antioxidant activities at 500 μg/mL according to their excellent temperature
stability. Ultimately, this study probes the novel approach of plant-mediated NPs
overcruel chemically incorporated NPs applied in pharmaceutical applications. ©
2023 Elsevier B.V.
AU - Balaji, V.
AU - Perumal, S.
AU - Palanisamy, S.
AU - Karuppaiah, M.
AU - Asaithambi, S.
AU - Velauthapillai, D.
AU - Kumar, P.
AU - Yuvakkumar, R.
AU - Ravi, G.
C7 - 171503
DB - Scopus
DO - 10.1016/j.jallcom.2023.171503
KW - MCF-7
MRSA
Reduced graphene oxide
Sidaacuta leaf
M3 - Article
PY - 2023
ST - Bio-inspired synthesis of silver nanoparticles and their nanocomposites for
antibacterial and anticancer activity: A comparative study
T2 - Journal of Alloys and Compounds
TI - Bio-inspired synthesis of silver nanoparticles and their nanocomposites for
antibacterial and anticancer activity: A comparative study
85166522611&doi=10.1016%2fj.jallcom.2023.171503&partnerID=40&md5=42300a4c3e59c63a4d
3eb6d39e20791d
VL - 966
ID - 5027
ER -
TY - JOUR
AB - The synthesis of metal nanoparticles by a green method is eco-friendly,
nontoxic and few benefits to the growing fields of research used inhibit bacteria
and cancer growth over the recent years. In this study, CuO NPs are synthesized
through Euphorbia hirta leaf extract. Formation of CuO NPs was confirmed by SPR
peak at UV spectrum. FTIR identified functional groups are responsible for
reduction and stabilization, GC-MS, phytochemicals present in the Euphorbia hirta
extract accountable for synthesizing CuO NPs. XRD confirmed crystalline nature, SEM
and TEM confirmed size and spherical morphology. Furthermore, CuO NPs effective
antibacterial activity to inhibit pathogens, strong biological activities regarding
antioxidant activity and anti-inflammatory activity and excellent anticancer
activity against the Ht-29 cell line. Also, good catalyst for the effective
degradation of methylene blue. The present study suggests that biosynthesized CuO
NPs through an extract from Euphorbia hirta may be used for therapeutic application
in future clinical studies.
AN - WOS:000678355100001
AU - Balakrishnan, V.
AU - Thangaraj, K.
AU - Palani, M.
AU - Vaiyapuri, M.
C6 - JUL 2021
DA - JUN 3
DO - 10.1080/24701556.2021.1952260
IS - 6
PY - 2022
SN - 2470-1556
2470-1564
SP - 809-818
ST - Green synthesis of copper oxide nanoparticles using Euphorbia hirta leaves
extract and its biological applications
T2 - INORGANIC AND NANO-METAL CHEMISTRY
TI - Green synthesis of copper oxide nanoparticles using Euphorbia hirta leaves
extract and its biological applications
VL - 52
ID - 6416
ER -
TY - JOUR
AB - Oral cancer is highly aggressive due to difficult diagnosis, therapy
resistance and increasing frequency; thus finding prevention therapies is very
important. This study evaluates the use of gold and silver nanoparticles (NPs),
phyto-synthesized with Cornus mas extract against oral dysplastic lesions. Methods:
NPs were characterized by UV-Vis, Fourier-transform infrared spectroscopy,
transmission electron microscopy, x-ray diffraction and laser Doppler
microelectrophoresis. Biological testing employed two human oral cell lines:
gingival fibroblasts and dysplastic keratinocytes and evaluated viability, cell
death mechanisms and cellular uptake. Results: NPs induced selective toxic effects
against dysplastic cells. p53/BAX/BCL2 activation and PI3K/AKT inhibition led to
cell death through necrosis and apoptosis. NPs also induced antioxidant and anti-
inflammatory effects. Conclusion: NPs of gold and silver showed promising
beneficial effects in the therapy of oral dysplasia. © 2020 Future Medicine Ltd.
AU - Baldea, I.
AU - Florea, A.
AU - Olteanu, D.
AU - Clichici, S.
AU - David, L.
AU - Moldovan, B.
AU - Cenariu, M.
AU - Achim, M.
AU - Suharoschi, R.
AU - Danescu, S.
AU - Vulcu, A.
AU - Filip, G. A.
DB - Scopus
DO - 10.2217/nnm-2019-0290
IS - 1
KW - apoptosis
cellular uptake
Cornus mas fruit
dysplastic oral keratinocytes
gold nanoparticles
green synthesis
oral cancer
p53/BAX/BCL2
bcl-2-Associated X Protein
Cell Line
Cell Survival
Cornus
Fibroblasts
Gingiva
Gold
Humans
Keratinocytes
Metal Nanoparticles
Mouth Neoplasms
Plant Extracts
Proto-Oncogene Proteins c-bcl-2
Silver
Tumor Suppressor Protein p53
Ultraviolet Rays
Cornus mas extract
gold nanoparticle
phosphatidylinositol 3 kinase
plant extract
protein Bax
protein bcl 2
protein kinase B
protein p53
silver nanoparticle
unclassified drug
BCL2 protein, human
gold
metal nanoparticle
silver
TP53 protein, human
Article
cell death
cell viability
cherry
controlled study
drug cytotoxicity
drug dosage form comparison
drug uptake
dysplasia
enzyme inhibition
fibroblast
fruit
gingiva
human
human cell
keratinocyte
laser Doppler microelectrophoresis
microelectrophoresis
nonhuman
oral cell line
oxidative stress
particle size
photosynthesis
Pi3K/Akt signaling
priority journal
spectrophotometry
Western blotting
X ray diffraction
zeta potential
cell line
cell survival
chemistry
drug effect
genetics
mouth tumor
pathology
ultraviolet radiation
M3 - Article
PY - 2019
SP - 55-75
ST - Effects of silver and gold nanoparticles phytosynthesized with Cornus mas
extract on oral dysplastic human cells
T2 - Nanomedicine
TI - Effects of silver and gold nanoparticles phytosynthesized with Cornus mas
85077172707&doi=10.2217%2fnnm-2019-
0290&partnerID=40&md5=c54b70748c9a357f624272e1096bfd2a
VL - 15
ID - 5401
ER -
TY - JOUR
AB - Background: Oral cancer is highly aggressive due to difficult diagnosis,
therapy resistance and increasing frequency; thus finding prevention therapies is
very important. Aim: This study evaluates the use of gold and silver nanoparticles
(NPs), phyto-synthesized with Cornus mas extract against oral dysplastic lesions.
Methods: NPs were characterized by UV-Vis, Fourier-transform infrared spectroscopy,
transmission electron microscopy, x-ray diffraction and laser Doppler
microelectrophoresis. Biological testing employed two human oral cell lines:
gingival fibroblasts and dysplastic keratinocytes and evaluated viability, cell
death mechanisms and cellular uptake. Results: NPs induced selective toxic effects
against dysplastic cells. p53/BAX/BCL2 activation and PI3K/AKT inhibition led to
cell death through necrosis and apoptosis. NPs also induced antioxidant and anti-
inflammatory effects. Conclusion: NPs of gold and silver showed promising
beneficial effects in the therapy of oral dysplasia.
AN - WOS:000504090800005
AU - Baldea, I.
AU - Florea, A.
AU - Olteanu, D.
AU - Clichici, S.
AU - David, L.
AU - Moldovan, B.
AU - Cenariu, M.
AU - Achim, M.
AU - Suharoschi, R.
AU - Danescu, S.
AU - Vulcu, A.
AU - Filip, G. A.
DA - JAN
DO - 10.2217/nnm-2019-0290
IS - 1
PY - 2020
SN - 1743-5889
1748-6963
SP - 55-75
ST - Effects of silver and gold nanoparticles phytosynthesized with Cornus mas
T2 - NANOMEDICINE
TI - Effects of silver and gold nanoparticles phytosynthesized with Cornus mas
VL - 15
ID - 6019
ER -
TY - JOUR
AB - Thiamethoxam is a broad-spectrum pesticide widely used in agricultural
practice throughout the world. Worryingly, this pesticide is considered a potential
contaminant on the surface and underground water, being a significant risk to
aquatic ecosystems and humans. In this sense, we decided to evaluate the activity
of enzymes belonging to purinergic system, which is linked with regulation of
extracellular nucleotides and nucleosides, as adenosine triphosphate (ATP) and
adenosine (Ado) molecules involved in the regulation of immune and inflammatory
responses. Such as the neurotoxic effects of thiamethoxam remain poorly understood,
the aim of this study was to evaluate whether purinergic signaling may be
considered a potential target of thiamethoxam-induced neurotoxicity in silver
catfish (Rhamdia quelen). Brain ectonucleoside triphosphate diphosphohydrolase (ATP
as substrate) and 5′-nucleotidases activities were inhibited at 3.75 µg L−1 after
24 h of exposure and at 1.125 and 3.75 µg L−1 after 96 h of exposure compared with
the control group. On the other hand, brain adenosine deaminase activity was
stimulated at 3.75 µg L−1 after 24 h of exposure and at 1.125 and 3.75 µg L−1 after
96 h of exposure compared with the control group. Brain ATP levels increased at
3.75 µg L−1 after 24 h of exposure and at 1.125 and 3.75 µg L−1 after 96 h of
exposure compared with the control group, while the Ado levels decreased. The
enzymatic activity of the purinergic signaling did not return to control levels
after a 48-h recovery period, revealing the potential neurotoxic effects of
thiamethoxam. In summary, the brain purinergic signaling may be considered a
potential target for thiamethoxam-induced neurotoxicity in silver catfish. © 2018,
AU - Baldissera, M. D.
AU - Souza, C. F.
AU - Golombieski, J. I.
AU - Seben, D.
AU - Sippert, L. R.
AU - Salbego, J.
AU - Marchesan, E.
AU - Zanella, R.
AU - Baldisserotto, B.
DB - Scopus
DO - 10.1007/s11010-018-3340-x
IS - 1-2
KW - Adenosine deaminase
Adenosine triphosphate
Inflammation
Pesticide
Adenosine
Adenosine Triphosphate
Animals
Brain
Catfishes
Fish Proteins
Neurotoxicity Syndromes
Signal Transduction
Thiamethoxam
5' nucleotidase
adenosine
adenosine deaminase
ectonucleoside triphosphate diphosphohydrolase
phosphatase
thiamethoxam
unclassified drug
fish protein
animal experiment
animal model
animal tissue
Article
catfish
controlled study
down regulation
enzyme activity
enzyme inhibition
female
hydrolysis
inflammation
male
neurotoxicity
nonhuman
Rhamdia quelen
signal transduction
animal
brain
disease model
drug effect
metabolism
pathology
toxicity and intoxication
M3 - Article
PY - 2018
SP - 39-45
ST - Purinergic signaling as potential target of thiamethoxam-induced
neurotoxicity using silver catfish (Rhamdia quelen) as experimental model
T2 - Molecular and Cellular Biochemistry
TI - Purinergic signaling as potential target of thiamethoxam-induced
neurotoxicity using silver catfish (Rhamdia quelen) as experimental model
85042523204&doi=10.1007%2fs11010-018-3340-
x&partnerID=40&md5=839f9f9db943b3bcb160283eb2d4aa05
VL - 449
ID - 5389
ER -
TY - JOUR
AB - Suitable control of immune and inflammatory responses is a critical
determinant of fish health, and the cholinergic system, through the enzyme
acetylcholinesterase (AChE), displays an important role in these responses by
regulation on acetylcholine (ACh) levels. In fish, exposure to aflatoxin B-1
(AEB(1)) is associated with reduced immune responsiveness and immunosuppression,
but the pathways involved on the impairment of fish immune system during AFB(1)
exposure remains poorly understood. Thus, the aim of this study was to evaluate
whether AChE activity in splenic tissue and immune cells is able to modulate the
inflammatory and immune responses in silver catfish (Rhamdia quelen) fed with a
diet contaminated by AFB(1). AChE activity in spleen tissue, as well as in
peripheric and splenic lymphocytes were lower on days 14 and 21 post-feeding in
animals fed with AFB(1) contaminated feed compared to the control group. Based on
these evidences, the results indicate that AChE activity is capable to modulate
immune and inflammatory responses in tissue and immune cells of fish fed with a
diet containing AFB(1), leading to the development of an anti-inflammatory profile
in an attempt to improve immune response and reduce or prevent tissue damage due to
the inflammatory response.
AN - WOS:000455344800002
AU - Baldissera, M. D.
AU - Souza, C. F.
AU - Zeppenfeld, C. C.
AU - Descovi, S. N.
AU - da Silva, A. S.
AU - Stefani, L. M.
DA - MAR 15
DO - 10.1016/j.aquaculture.2018.12.023
PY - 2019
SN - 0044-8486
1873-5622
SP - 8-11
ST - Modulation of acetylcholinesterase activity exerts anti-inflammatory effect
in spleen and immune cells of fish fed with a diet contaminated by aflatoxin B-1
T2 - AQUACULTURE
TI - Modulation of acetylcholinesterase activity exerts anti-inflammatory effect
in spleen and immune cells of fish fed with a diet contaminated by aflatoxin B-1
VL - 502
ID - 6181
ER -
TY - JOUR
AB - Two new mixed ligand-silver(i) complexes of the anti-inflammatory drug
naproxen (naprH) and triphenylphosphine (tpp) or tri(p-tolyl)phosphine (tptp) of
formulae {[Ag(tpp)3(napr)](H2O)} (1) and [Ag(tptp) 2(napr)] (2) have been
synthesized and characterized by m.p., vibrational spectroscopy (mid-FT-IR), Raman,
1H-NMR, UV-Vis, ESI-MS spectroscopic techniques and X-ray crystallography. The
complexes show high photo-sensitivity to UVC light. Photolysis of 1-2 was studied
and the results showed monotonic degradation of the complexes with simultaneous
triarylphosphine oxide formation. The complexes 1-2 were tested for their
antiproliferative activity against human breast adenocarcinoma (MCF-7) cells.
Complexes 1-2 were more active than cisplatin against cells. UVC light increases
the effectiveness of complexes 1-2 on MCF-7 cells by 13% and 38% respectively. Due
to the morphology of the MCF-7 cells, which were incubated with the complexes 1-2,
the cell death was ascribed to apoptosis. Electrophoresis to genomic DNA of MCF-7
cells confirmed the apoptosis through DNA fragmentation. The binding affinity of 1-
2 towards the intracellular molecules CT-DNA and lipoxygenase (LOX) was studied for
the evaluation of the mechanism of cell death. Thus, the binding constants (Kb) of
1-2 towards CT-DNA calculated by UV-Vis spectra are 32.8 ± 8.5 × 104 (1) and 4.7 ±
1.8 × 104 (2) M-1, respectively. Changes in fluorescent emission light of ethidium
bromide (EB) in the presence of DNA suggest intercalation or electrostatic
interactions into DNA of both complexes 1-2 in the minor groove. The corresponding
apparent binding constants (Kapp) of 1-2 towards CT-DNA calculated through
fluorescence spectra are 2.9 ± 0.3 × 104 (1) and 1.6 ± 0.4 × 104 (2) M -1
respectively. Docking studies on DNA-complexes interactions show the binding of 1
in the major groove and the corresponding one of 2 in the minor one. Moreover, the
influence of complexes 1-2 on the catalytic peroxidation of linoleic acid to
hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX) was kinetically and
theoretically studied. Only 1 inhibits lipoxygenase activity (IC50 = 5.1 (1), >30
(2) μM). This journal is © the Partner Organisations 2014.
AU - Banti, C. N.
AU - Giannoulis, A. D.
AU - Kourkoumelis, N.
AU - Owczarzak, A. M.
AU - Kubicki, M.
AU - Hadjikakou, S. K.
DB - Scopus
DO - 10.1039/c3dt53175a
IS - 18
KW - Anti-Inflammatory Agents, Non-Steroidal
Apoptosis
Humans
MCF-7 Cells
Molecular Docking Simulation
Naproxen
Protein Binding
Protein Structure, Secondary
Silver
Binding energy
Cell death
DNA
Electrophoresis
Fluorescence
Linoleic acid
Phosphorus compounds
Photodegradation
Photolysis
Platinum compounds
X ray crystallography
naproxen
protein binding
silver
Anti-inflammatory drugs
Binding affinities
Fluorescence spectra
Fluorescent emission
Intracellular components
Spectroscopic technique
Triphenylphosphines
apoptosis
chemistry
human
MCF 7 cell line
metabolism
molecular docking
physiology
procedures
protein secondary structure
Complexation
M3 - Article
PY - 2014
SP - 6848-6863
ST - Novel metallo-therapeutics of the NSAID naproxen. Interaction with
intracellular components that leads the cells to apoptosis
T2 - Dalton Transactions
TI - Novel metallo-therapeutics of the NSAID naproxen. Interaction with
intracellular components that leads the cells to apoptosis
84897036333&doi=10.1039%2fc3dt53175a&partnerID=40&md5=a31715c7a0274456900c0bcfd18c5
c48
VL - 43
ID - 5655
ER -
TY - JOUR
AB - A new mixed Iigand-silver(I) complex of formula [Ag(tpp)2(p-Hbza)] (1) (p-
HbzaH = 4-hydroxybenzoic acid and tpp = triphenylphosphine) has been synthesized
and characterized by elemental analysis, mp, vibrational spectroscopy (mid- and
far-FT-IR), 1H-NMR, UV-vis, ESI-MS spectroscopic techniques and X-ray
crystallography. Complex 1 and the already known mixed Iigand-silver(I) complexes
of formulae [Ag(tpp)2(salH)] (2) (salH2 = salicylic acid or 2-hydroxy-benzoic acid)
and {[Ag(tpp)3(asp)](dmf)} (3) (aspH = o-acetylsalicylic acid) were used for the
clarification of the cytostatic activity mechanism. Thus, 1-3 were tested for their
in vitro cytotoxic activity against leiomyosarcoma (LMS) and human breast
adenocarcinoma (MCF-7) cells with trypan blue and Thiazolyl Blue Tetrazolium
Bromide (MTT) assays. For both cell lines, complexes 1-3 were found to be more
active than cisplatin. Due to the morphology of the LMS cells after incubation with
1-3, the type of cell death was evaluated by flow cytometry assay and DNA
fragmentation. The results show that LMS cells undergo programmed cell death
(apoptosis). DNA binding tests indicate the ability of complexes 1-3 to modify the
activity of the cells. The binding constants of 1-3 towards calf-thymus DNA (CT-
DNA) ((27.7 ± 7.9) × 104 (1), (13.3 ± 6.5) × 104 (2) and (11 ± 2.8) × 10 4 (3) M-1)
indicate strong interaction. Moreover, the influence of complexes 1-3 on the
catalytic peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme
lipoxygenase (LOX) was kinetically studied. Finally, docking studies on DNA binding
interactions were performed. © The Royal Society of Chemistry 2012.
AU - Banti, C. N.
AU - Giannoulis, A. D.
AU - Owczarzak, A. M.
AU - Poyraz, M.
AU - Kubicki, M.
AU - Charalabopoulos, K.
DB - Scopus
DO - 10.1039/c2mt20039b
IS - 6
KW - Animals
Apoptosis
Binding Sites
Cattle
Cell Line, Tumor
Cell Shape
Cell Survival
Coordination Complexes
DNA
Flow Cytometry
Humans
Kinetics
Linoleic Acid
Lipid Peroxidation
Lipoxygenase
Models, Molecular
Organophosphorus Compounds
Parabens
Protein Binding
Silver
Assays
Binding energy
Bromine compounds
Carboxylic acids
Cell culture
Cell death
Flow cytometry
Linoleic acid
Platinum compounds
Silver compounds
4 hydroxybenzoic acid
cisplatin
linoleic acid
lipoxygenase
metal complex
salicylic acid
silver derivative
triphenylphosphine
4 hydroxybenzoic acid ester
4-hydroxybenzoic acid
calf thymus dna
coordination compound
organophosphorus compound
silver
4-hydroxybenzoic acids
Anti-inflammatory agents
Binding constant
Calf thymus DNA
Cell lines
Cis-platin
Complex 1
Cytostatic activities
DNA binding
DNA fragmentation
Flow cytometry assays
Human breast
In-vitro
Leiomyosarcoma
Lipoxygenases
Peroxidation
Programmed cell deaths
Salicylic acids
Strong interaction
Triphenyl phosphines
apoptosis
article
binding affinity
binding competition
breast adenocarcinoma
cancer cell culture
catalysis
cell death
cell proliferation
cell structure
cell viability
competitive inhibition
controlled study
crystal structure
cytotoxicity
enzyme substrate
flow cytometry
human
human cell
in vitro study
leiomyosarcoma
lipid peroxidation
molecular docking
priority journal
proton nuclear magnetic resonance
animal
binding site
cattle
cell shape
cell survival
chemical structure
chemistry
drug effect
kinetics
metabolism
protein binding
tumor cell line
Complexation
M3 - Article
PY - 2012
SP - 545-560
ST - Mixed ligand-silver(I) complexes with anti-inflammatory agents which can bind
to lipoxygenase and calf-thymus DNA, modulating their function and inducing
apoptosis
T2 - Metallomics
TI - Mixed ligand-silver(I) complexes with anti-inflammatory agents which can bind
to lipoxygenase and calf-thymus DNA, modulating their function and inducing
apoptosis
84866468739&doi=10.1039%2fc2mt20039b&partnerID=40&md5=91d8524fbaba047d4b59772c3ba6b
a47
VL - 4
ID - 5667
ER -
TY - JOUR
AB - Silver is proving to have a number of medicinal applications; as an
antiseptic, an antibacterial, and an anti-inflammatory, while any biological role
for it is currently unknown. Silver compounds and their therapeutic potentials are
under consideration from many research groups, while a number of early reviews
recording the advances of silver(i) chemistry are also available. However there is
no recent report on the screening for the antitumor potential of silver(i)
compounds. This review focuses upon results obtained on the anti-proliferative
activity of silver compounds in the past years. This survey shows that silver(i)
complexes containing various type of ligands such as carboxylic acids, amino acids,
nitrogen, phosphorus or sulfur donor ligands, exhibit selectivity against a variety
of cancer cells. The role of the coordination number, which is related to either
the stability or hydrophilicity-lipophilicity of a complex, is not clearly
elucidated within this review. © The Royal Society of Chemistry 2013.
AU - Banti, C. N.
DB - Scopus
DO - 10.1039/c3mt00046j
IS - 6
KW - Animals
Cell Proliferation
Humans
Models, Biological
Silver Compounds
Amino acids
Diagnosis
Functional groups
Ligands
6 hydroxycoumarin 3 carboxylatosilver
acetic acid derivative
acetylcysteine derivative
acetylsalicylic acid derivative
adamantane derivative
alkane derivative
benzimidazole derivative
benzoic acid derivative
cisplatin
coumarin derivative
diamine derivative
dicarboxylic acid derivative
hydantoin derivative
hydrazide derivative
hydroxybenzoic acid derivative
malonic acid derivative
mesylic acid derivative
phenanthroline derivative
phosphine derivative
phosphonic acid derivative
pyrimidine derivative
silver derivative
sulfur derivative
thiophene derivative
thiosemicarbazone derivative
tryptophan derivative
unclassified drug
unindexed drug
Anti-inflammatories
Anti-proliferative
Anti-tumor activities
Coordination number
Medicinal applications
Silver complexes
antiproliferative activity
binding affinity
cancer cell
carcinoma cell
cell viability
chronic myeloid leukemia
colon adenocarcinoma
cytostasis
DNA synthesis
drug cytotoxicity
drug screening
drug selectivity
drug stability
Ehrlich ascites tumor cell
fluorescence
human
hydrophilicity
IC 50
kidney carcinoma
lipophilicity
mastocytoma
melanoma
melanoma B16
neuroblastoma
nonhuman
nuclear magnetic resonance spectroscopy
ovary carcinoma
pancreas carcinoma
priority journal
R factor
review
squamous cell carcinoma
X ray diffraction
Silver compounds
M3 - Review
PY - 2013
SP - 569-596
ST - Anti-proliferative and anti-tumor activity of silver(i) compounds
T2 - Metallomics
TI - Anti-proliferative and anti-tumor activity of silver(i) compounds
84878796988&doi=10.1039%2fc3mt00046j&partnerID=40&md5=aa56e4a2e3b0a68ef8b41026b2ded
fdc
VL - 5
ID - 5704
ER -
TY - JOUR
AB - The conjugation of diclofenac (DICLH), a Non-Steroidal Anti-inflammatory Drug
(NSAID), with biocides such as dimethyl sulfoxide (DMSO) and triphenylphosphine
(TPP), through silver(I) ions, results into the chemical [Ag n (DICL) n (L) m ] k
(L = DMSO and n = 2, m = 2, k = infinite (1); L = TPP and n = 1, m = 2, k = 1 (2)).
The compounds were characterized by m.p., FT-IR, UV–vis and 1 H NMR spectroscopic
techniques. The crystal and molecular structures of 1–2 were determined by X-ray
crystallography. The in vitro cytotoxic activity of 1–2 against the human breast
adenocarcinoma cancer cells MCF-7 (hormone dependent) and MDA-MB-231 (hormone
independent) reveals that the 1 inhibits the MCF-7 rather than the MDA-MB-231
cells, suggesting hormone mimetic behaviour. Compound 2 inhibits both cancerous
cell lines, stronger than cisplatin. Both compounds inhibit MCF-7 cells migration.
Compounds 1–2, exhibit, lower toxicity against fetal lung fibroblast (MRC-5) cells
than cisplatin. Their genotoxicity was evaluated on MRC-5 cells. The molecular
mechanism of 1–2 against MCF-7 cells was clarified by (i) their cell cycle arrest
study (ii) their mitochondrial membrane permeability (iii) their binding affinity
towards Calf Thymus (CT)-DNA and (iv) their inhibitory activity against the enzyme
lipoxygenase (LOX). Regression analysis of the data obtained for [Ag(NSAID)(Ar 3 P)
m ] (NSAID = p-hydroxy-benzoic acid (p-HO-BZAH), salicylic acid (SALH 2 ), aspirin
(ASPH), naproxen (NAPRH), nimesulide (NIMH); L = TPP, Tri(p-tolyl)phosphine (TPTP),
Tri(o-tolyl)phosphine (TOTP), Tri(m-tolyl)phosphine (TMTP); m = 2 or 3) and
[Ag(DICL) 2 (DMSO) 2 ] k (k = infinite) was performed. Considering the biological
results (IC 50 ) as dependent variable a theoretical equation is obtained for these
compounds. The calculated IC 50 values are compared satisfactorily with the
corresponding experimental inhibitory activity of the complexes. © 2019 Elsevier
Inc.
AU - Banti, C. N.
AU - Hatzidimitriou, A. G.
DB - Scopus
DO - 10.1016/j.jinorgbio.2019.01.020
KW - Bioinorganic chemistry
Cytotoxicity
Diclofenac
Drugs development
Regression analysis
Silver(I)
Animals
Anti-Inflammatory Agents, Non-Steroidal
Cattle
Cell Line, Tumor
Cell Movement
Cell Proliferation
Disinfectants
DNA
Humans
Ligands
Lipoxygenase Inhibitors
Mitochondria
Multivariate Analysis
Regression Analysis
S Phase Cell Cycle Checkpoints
Silver
cisplatin
diclofenac
dimethyl sulfoxide
naproxen
nimesulide
salicylic acid
silver
tri(2 tolyl)phosphine
triphenylphosphine
unclassified drug
calf thymus DNA
disinfectant agent
ligand
lipoxygenase inhibitor
apoptosis
Article
binding affinity
cell migration
chemical structure
crystallization
cytotoxicity
DNA binding
drug conformation
drug conjugation
drug formulation
G2 phase cell cycle checkpoint
genetic damage
genotoxicity
human
human cell
hydrogen bond
IC50
in vitro study
lipophilicity
M phase cell cycle checkpoint
MCF-7 cell line
MDA-MB-231 cell line
micronucleus
mitochondrial permeability
molecular weight
MRC-5 cell line
prediction
S phase cell cycle checkpoint
solid state
structure activity relation
vibrational spectroscopy
animal
bovine
cell motion
cell proliferation
drug effect
mitochondrion
multivariate analysis
regression analysis
synthesis
tumor cell line
M3 - Article
PY - 2019
SP - 7-18
ST - Diclofenac conjugates with biocides through silver(I) ions (CoMeD's);
Development of a reliable model for the prediction of anti-proliferation of
NSAID's-silver formulations
T2 - Journal of Inorganic Biochemistry
TI - Diclofenac conjugates with biocides through silver(I) ions (CoMeD's);
Development of a reliable model for the prediction of anti-proliferation of
NSAID's-silver formulations
85061825166&doi=10.1016%2fj.jinorgbio.2019.01.020&partnerID=40&md5=11b7748f7b92b5d4
04d7df546140163c
VL - 194
ID - 5394
ER -
TY - JOUR
AB - Novel silver(I) metallo-drugs of the nonsteroidal anti-inflammatory drug
nimesulide (nim) and the mitochondriotropic triaryl derivatives of pnictogen
ligands (tpE, E = P (tpp, tptp, or totp), As (tpAs), Sb (tpSb)) with the formulas
{[Ag(nim) (tpp)2]DMF} (1), [Ag(nim) (tptp)2] (2), [Ag(nim) (totp)] (3), [Ag(nim)
(tpAs)2] (4), and [Ag(nim) (tpSb)3] (5) ((tpp = triphenyphosphine, tptp = tri(p-
tolyl)phosphine, totp = tri(o-tolyl)phosphine, tpAs = triphenylarsine, tpSb =
triphenylantimony, and DMF = dimethylformamide) were synthesized and characterized
by melting point, vibrational spectroscopy (mid-Fourier transform IR), 1H NMR, UV-
visible spectroscopic techniques, and X-ray crystallography. The in vitro cytotoxic
activity of 1-5 against human breast adenocarcinoma cancer cell lines: MCF-7
(estrogen receptor (ER) positive) and MDA-MB-231 (ER negative) was determined. The
genotoxicity on normal human fetal lung fibroblast cells (MRC-5) caused by 1-5 was
evaluated by fluorescence microscopy. The absence of micronucleus in MRC-5 cells
confirms the in vitro non toxicity behavior of the compounds. Because of the
morphology of the cells, an apoptotic pathway was concluded for the cell death. The
apoptotic pathway, especially though the mitochondrion damage, was confirmed by DNA
fragmentation, cell cycle arrest, and permeabilization of the mitochondrial
membrane tests. The molecular mechanism of action of 1-5 was further studied by (i)
the binding affinity of 1-5 toward the calf thymus (CT) DNA, (ii) the inhibitory
activity of 1-5 against lipoxygenase (an enzyme that oxidizes polyunsaturated fatty
acids to leukotrienes or prostaglandins), and (iii) the catalytic activity of 1-5
on the oxidation of linoleic acid (an acid that partakes in membrane fluidity,
membrane enzyme activities, etc.) to hyperoxolinoleic acid by oxygen. © 2016
American Chemical Society.
AU - Banti, C. N.
AU - Papatriantafyllopoulou, C.
AU - Manoli, M.
AU - Tasiopoulos, A. J.
DB - Scopus
DO - 10.1021/acs.inorgchem.6b01241
IS - 17
KW - Antineoplastic Agents
Apoptosis
Breast Neoplasms
Cell Cycle Checkpoints
Cell Line
Cell Line, Tumor
DNA Fragmentation
Humans
MCF-7 Cells
Models, Molecular
Silver
Sulfonamides
nimesulide
silver
sulfonamide
apoptosis
breast tumor
cell cycle checkpoint
cell line
chemistry
DNA fragmentation
drug effects
human
MCF-7 cell line
molecular model
pathology
tumor cell line
M3 - Article
PY - 2016
SP - 8681-8696
ST - Nimesulide Silver Metallodrugs, Containing the Mitochondriotropic, Triaryl
Derivatives of Pnictogen; Anticancer Activity against Human Breast Cancer Cells
T2 - Inorganic Chemistry
TI - Nimesulide Silver Metallodrugs, Containing the Mitochondriotropic, Triaryl
Derivatives of Pnictogen; Anticancer Activity against Human Breast Cancer Cells
84986239365&doi=10.1021%2facs.inorgchem.6b01241&partnerID=40&md5=b91f1b55801172c0f5
455ee56d10a7a7
VL - 55
ID - 5502
ER -
TY - JOUR
AB - {[Ag8(Mef)8(μ2-S,O-DMSO)2(μ2-O-DMSO)2(O-DMSO)8]·2(H2O)} (1), [Ag(Mef)(tpP)2]
(2), [Ag(Mef)(tpAs)3] (3), and {2 [Ag(Mef)(tpSb)3] (DMSO)} (4) were obtained by the
conjugation of mefenamic acid (MefH), a nonsteroidal anti-inflammatory drug
(NSAID), with a mitochondriotropic derivative of pnictogen tpE (tp = triphenyl
group; E = P, As, and Sb) through silver(I). Their hydrophilicity was adjusted by
their dispersion into sodium lauryl sulfate (SLS), forming SLS@1-4. 1-4 and SLS@1-4
were characterized by their spectral data and X-ray crystallography. They inhibit
the proliferation of human breast adenocarcinoma cells MCF-7 (hormone-dependent
(HD)) and MDA-MB-231 (hormone-independent (HI)). X-ray fluorescence reveals the Ag
cellular uptake. The in vitro and in vivo nongenotoxicity was confirmed with
micronucleus (MN), Artemia salina, and Allium cepa assays. Their mechanism of
action was studied by cell morphology, DNA fragmentation, acridine orange/ethidium
bromide (AO/EB) staining, cell cycle arrest, mitochondrial membrane
permeabilization tests, DNA binding affinity, and LOX inhibitory activity and was
rationalized by regression analysis. © 2023 American Chemical Society.
AU - Banti, C. N.
AU - Papachristodoulou, C.
DB - Scopus
DO - 10.1021/acs.jmedchem.2c02126
IS - 6
KW - Anti-Inflammatory Agents
Apoptosis
Cell Line, Tumor
Dimethyl Sulfoxide
Hormones
Humans
4 hydrobenzoic acid
acridine orange
aldo keto reductase
aneugen
antimony
apoptosis inducing factor
arsenic
benzene derivative
carbonyl derivative
carboxyl group
chelate
cisplatin
clastogen
diclofenac
disulfide
dodecyl sulfate sodium
drug carrier
environmental marker
ethidium bromide
glutathione
linoleic acid
lipoxygenase
mefenamic acid
naproxen
nimesulide
phosphorus
pnictogen derivative
reduced nicotinamide adenine dinucleotide phosphate
salicylic acid
silver
triphenylantimony
triphenylarsine
triphenylphosphine
unclassified drug
dimethyl sulfoxide
hormone
apoptosis
aqueous solution
Artemia salina
Article
association constant
base pairing
binding affinity
cell cycle arrest
cell structure
cell viability
chemical structure
chromosome damage
conjugation
controlled study
critical micelle concentration
crystal structure
cytotoxicity
deprotonation
dispersion
DNA binding
DNA fragmentation
drug conjugation
drug design
drug mechanism
enzyme activity
enzyme inhibition
genotoxicity
human
human cell
hydrogen bond
hydrophilicity
IC50
in vitro study
in vivo study
larva
lethality
MCF-7 cell line
micelle
micronucleus
mitosis index
MRC-5 cell line
nonhuman
onion
peroxidation
substitution reaction
synergistic effect
toxicity testing
viscometry
X ray fluorescence
X ray fluorescence spectrometry
chemistry
tumor cell line
M3 - Article
PY - 2023
SP - 4131-4149
ST - New Apoptosis Inducers Containing Anti-inflammatory Drugs and Pnictogen
Derivatives: A New Strategy in the Development of Mitochondrial Targeting
Chemotherapeutics
T2 - Journal of Medicinal Chemistry
TI - New Apoptosis Inducers Containing Anti-inflammatory Drugs and Pnictogen
Derivatives: A New Strategy in the Development of Mitochondrial Targeting
Chemotherapeutics
85147948975&doi=10.1021%2facs.jmedchem.2c02126&partnerID=40&md5=92c87ba5f9d5f041b34
e063ccba529c3
VL - 66
ID - 5036
ER -
TY - JOUR
AB - The non steroidal anti-inflammatory drugs (NSAID's)-silver(I) metallodrugs of
aspirin (aspH), salicylic acid (salH2), naproxen (napH) acid or p-hydrobenzoic acid
(pHbzaH) and the mitochondriotropic triphenylarsine (tpAs) with the formulae
[Ag(asp)(tpAs)3] (1), [Ag(salH)(tpAs)3] (2), [Ag(nap)(tpAs)3] (3) and {[Ag(pHbza)
(tpAs)3]∙(dmf)} (4) and [Ag(tpAs)3(NO3)] (5) have been synthesized and
characterized by m.p., FT-IR, UV-vis and 1H NMR, spectroscopic techniques and X-ray
crystallography. The in vitro cytotoxic activity of 1–5 against human breast
adenocarcinoma cancer cells: MCF-7 (positive to estrogen receptors (ERs)) and MDA-
MB-231 (negative to estrogen receptors (ERs)) was evaluated. Compound 4 exhibits
the stronger activity against MCF-7 (2.5 ± 0.1 μΜ), while 1 the strongest one
against MDA-MB-231 (3.2 ± 0.3 μΜ). The IC50 values against normal human fetal lung
fibroblast cells lie between 3.0 and 3.7 μΜ. The toxic effect of 1–5 was evaluated
against normal human fetal lung fibroblast cells (MRC-5 cells). The IC50 values of
1–5 lie between 2.9 and 3.7 μΜ. The genotoxicity or not of 1–5 against MRC-5 cells
was detected from the presence or absence of micronucleus using fluorescence
microscopy. The presence of micronucleus in MRC-5 cells (3.0–3.7% in contrast to 1%
of the untreated cells) confirms the in vitro toxic behaviour of the compounds. The
apoptotic pathway, though the mitochondrion, was confirmed by cell cycle arrest
(increasing of the apoptotic cells, in sub-G1 phase (3.5 (5) - 13.3% (4)) in
contrast of 1.8% in the control group) and permeabilization of the mitochondrial
membrane test (MMP assay). Moreover, the ability of 1–5 to interact with Calf
Thymus (CT)-DNA was also studied. Compound 4 exhibits the highest DNA binding
constant (Kb= (25.0 ± 9.7) × 104 M−1). The inhibitory activity of 1–5 against the
enzyme lipoxygenase (LOX) is also investigated. The activity order is 1 > 4 > 3 >
2,5. © 2017 Elsevier Masson SAS
AU - Banti, C. N.
DB - Scopus
DO - 10.1016/j.ejmech.2017.10.067
KW - Biological inorganic chemistry
Cytotoxicity
Drugs design
NSAIDs
Silver(I)
Breast Neoplasms
Cell Line
Cell Membrane Permeability
Cell Proliferation
Drug Screening Assays, Antitumor
Female
Humans
Mitochondrial Membranes
Molecular Structure
Structure-Activity Relationship
4 hydrobenzoic acid
mitochondriotropic triphenylarsine
naproxen
salicylic acid
unclassified drug
Article
breast cancer
cell cycle G1 phase
controlled study
cytotoxicity
drug synthesis
fetus
fluorescence microscopy
Fourier transform infrared photoacoustic spectroscopy
genotoxicity
human
human cell
IC50
in vitro study
lung fibroblast
MCF-7 cell line
micronucleus
mitochondrion
MRC-5 cell line
breast tumor
cell line
cell membrane permeability
cell proliferation
chemical structure
chemistry
dose response
drug effects
drug screening
female
pathology
synthesis
M3 - Article
PY - 2018
SP - 1687-1701
ST - New metalo-therapeutics of NSAIDs against human breast cancer cells
T2 - European Journal of Medicinal Chemistry
TI - New metalo-therapeutics of NSAIDs against human breast cancer cells
85033559611&doi=10.1016%2fj.ejmech.2017.10.067&partnerID=40&md5=014861bb51a6b0309a6
89ddec96d98cb
VL - 143
ID - 5557
ER -
TY - JOUR
AB - Uveitis is an inflammation of the middle layer of the eye with a high risk of
blindness. The Gi protein associated A(3) adenosine receptor (A(3)AR) is highly
expressed in inflammatory cells whereas low expression is found in normal cells.
CF101 is a highly specific agonist at the A(3)AR known to induce a robust anti-
inflammatory effect in different experimental animal models. The CF101 mechanism of
action entails down-regulation of the NF-kappa B-TNF-alpha signaling pathway,
resulting in inhibition of pro-inflammatory cytokine production and apoptosis of
inflammatory cells. In this study the effect of CF101 on the development of retinal
antigen interphotoreceptor retinoid-binding protein (IRBP)-induced experimental
autoimmune uveitis (EAU) was assessed. Oral treatment with CF101 (10 mu g/kg, twice
daily), initiated upon disease onset, improved uveitis clinical score measured by
fundoscopy and ameliorated the pathological manifestations of the disease. Shortly
after treatment with CF101 A(3)AR expression levels were down-regulated in the
lymph node and spleen cells pointing towards receptor activation. Downstream events
included a decrease in PI3K and STAT-1 and proliferation inhibition of IRPB auto-
reactive T cells ex vivo. Inhibition of interleukin-2, tumor necrosis factor-alpha
(TINF-alpha) and interferon-gamma (IFN-gamma) production and up-regulation of
interleukin-10 was found in cultured splenocytes derived from CF101-treated
animals. Overall, the present study data point towards a marked anti-inflammatory
effect of CF101 in EAU and support further exploration of this small molecule drug
for the treatment of uveitis.
AN - WOS:000295422600007
AU - Bar-Yehuda, S.
AU - Luger, D.
AU - Ochaion, A.
AU - Cohen, S.
AU - Patokaa, R.
AU - Zozulya, G.
AU - Silver, P. B.
AU - De Morales, Jmgr
AU - Caspi, R. R.
AU - Fishman, P.
DA - NOV
DO - 10.3892/ijmm.2011.753
IS - 5
PY - 2011
SN - 1107-3756
1791-244X
SP - 727-731
ST - Inhibition of experimental auto-immune uveitis by the A3 adenosine receptor
agonist CF101
T2 - INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
TI - Inhibition of experimental auto-immune uveitis by the A3 adenosine receptor
agonist CF101
VL - 28
ID - 6560
ER -
TY - JOUR
AB - The silver nanoparticles (AgNPs) prepared by chemical reduction with sodium
hypophosphite as a reducing agent and sodium hexametaphosphate as a stabilising
agent were highly cytotoxic against human cells (U-937 and HL-60). The aim of the
study was to determine the impact of selected antioxidants: ascorbic acid (AA),
gallic acid (GA), scavenger (trolox (TX)) and Ag + chelator (N-acetylcysteine, NAC)
on viability, modulation of inflammatory response and apoptosis index of cells
treated by AgNPs. Selected protectants added individually or together affects the
viability of cells treated by AgNPs (1 mg/L). The mixtures assuring the most
efficient defense against AgNPs were: AgNPs + TX + AA, AgNPs + GA + AA, AgNPs + TX
+ GA + AA and AgNPs + TX + GA + AA + NAC which synergistically interact in the
mixture. The greatest reduction in IL-6 and TNF-α levels was found for the mixture
containing AgNPs + TX + GA + AA. Mixture of this composition exhibited also the
strongest anti-apoptotic effect. Highly cytotoxic AgNPs may not damage human cells
if cytoprotectants are present. © 2019, © 2019 Informa UK Limited, trading as
AU - Barbasz, A.
AU - Czyżowska, A.
DB - Scopus
DO - 10.1080/08927022.2019.1566649
IS - 7
KW - antioxidants
human cells
Antioxidants
Cell death
Metal nanoparticles
Mixtures
Sodium compounds
Anti-apoptotic effects
Chemical reduction
Human cells
Sodium hexametaphosphate
Sodium hypophosphite
Stabilising agents
Ascorbic acid
M3 - Article
PY - 2019
SP - 585-594
ST - Is there a way to protect human immune cells against nanocytotoxicity?
T2 - Molecular Simulation
TI - Is there a way to protect human immune cells against nanocytotoxicity?
85060184139&doi=10.1080%2f08927022.2019.1566649&partnerID=40&md5=b78b4c5dfa794e43f2
f05e25034fe488
VL - 45
ID - 5358
ER -
TY - JOUR
AB - The silver nanoparticles (AgNPs) prepared by chemical reduction with sodium
hypophosphite as a reducing agent and sodium hexametaphosphate as a stabilising
agent were highly cytotoxic against human cells (U-937 and HL-60). The aim of the
study was to determine the impact of selected antioxidants: ascorbic acid (AA),
gallic acid (GA), scavenger (trolox (TX)) and Ag+ chelator (N-acetylcysteine, NAC)
on viability, modulation of inflammatory response and apoptosis index of cells
treated by AgNPs. Selected protectants added individually or together affects the
viability of cells treated by AgNPs (1 mg/L). The mixtures assuring the most
efficient defense against AgNPs were: AgNPs + TX + AA, AgNPs + GA + AA, AgNPs + TX
+ GA + AA and AgNPs + TX + GA + AA + NAC which synergistically interact in the
mixture. The greatest reduction in IL-6 and TNF-alpha levels was found for the
mixture containing AgNPs + TX + GA + AA. Mixture of this composition exhibited also
the strongest anti-apoptotic effect. Highly cytotoxic AgNPs may not damage human
cells if cytoprotectants are present.
AN - WOS:000462167900006
AU - Barbasz, A.
AU - Czyzowska, A.
DA - MAY 3
DO - 10.1080/08927022.2019.1566649
IS - 7
PY - 2019
SN - 0892-7022
1029-0435
SP - 585-594
ST - Is there a way to protect human immune cells against nanocytotoxicity?
T2 - MOLECULAR SIMULATION
TI - Is there a way to protect human immune cells against nanocytotoxicity?
VL - 45
ID - 6077
ER -
TY - JOUR
AB - Gold nanoparticles (AuN) are one of the most investigated nanomaterials,
finding numerous applications from medicine to industry. AuN were obtained by
reduction of gold salts using tannic acid as a reducing agent. To detach the impact
of AuN onto cells of two lines human monocytic cells (U-937) and human
promyelocytic leukaemia cells (HL-60), the effects of postreaction residues (of
effluent from sol cleaning) and gold ions were also examined. It was demonstrated
that resistance of cells to the toxic action of AuN is dependent not only on
incubation time and dosage, but also on stages of cell differentiation. It was
found that after incubation of promonocytes U-937 with 25ppm AuN, the cell
viability decreased by 25% and of macrophages by 50%. Differentiated cells U-937
showed a significantly lower resistance than the not-differentiated cells. For HL-
60 cells, regardless of differentiation, cell viability decreased by approximately
20% after treatment with 25ppm AuN sol. It was noticed that U-937 exhibited higher
vulnerability to AuN than HL-60 cells. It was proved that AuN induced over a 15-
fold increase in nitric oxide and a decrease of intracellular glutathione levels
indicating the inflammatory response of cells. Even though gold ions did not show a
significant effect on cell viability, they caused fivefold increase of nitric oxide
level. The results show a higher cytotoxicity of AuN than gold ions. An overall
picture of the interaction of AuN with human cells of first defence line was
obtained. The results indicate that AuN may cause changes in the response of
phagocytes in inflammatory conditions.
AN - WOS:000372089700010
AU - Barbasz, A.
AU - Ocwieja, M.
DA - MAY 2
DO - 10.1080/17458080.2015.1096024
IS - 7
PY - 2016
SN - 1745-8080
1745-8099
SP - 564-580
ST - Gold nanoparticles and ions - friends or foes? As they are seen by human
cells U-937 and HL-60
T2 - JOURNAL OF EXPERIMENTAL NANOSCIENCE
TI - Gold nanoparticles and ions - friends or foes? As they are seen by human
cells U-937 and HL-60
VL - 11
ID - 6485
ER -
TY - JOUR
AB - The properties of silver nanoparticles (AgNPs) synthesized using compounds
exhibiting biological activity seem to constitute an interesting issue worthy of
examination. In these studies, two types of AgNPs were synthesized by a chemical
reduction method using well-known antioxidants: gallic acid (GA) and ascorbic acid
(AA). Transmission electron microscopy (TEM) and atomic force microscopy (AFM)
revealed that the AgNPs were spherical. The average size was equal to 26 ± 6 nm and
20 ± 7 nm in the case of ascorbic acid-silver nanoparticles (AAgNPs) and gallic
acid-silver nanoparticles (GAAgNPs), respectively. Surface-enhanced Raman
spectroscopy (SERS) confirmed that the AgNPs were not stabilized by pure forms of
applied antioxidants. Changes in mitochondrial activity and secretion of
inflammatory and apoptosis mediators after the exposure of human promyelocytic (HL-
60) and histiocytic lymphoma (U-937) cells to the AgNPs were studied to determine
the impact of stabilizing layers on nanoparticle toxicity. The GAAgNPs were found
to be more toxic for the cells than the AAgNPs. Their toxicity was manifested by a
strong reduction in mitochondrial activity and induction of the secretion of
interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and caspase-9. The addition
of pure antioxidants to the AgNP suspensions was found to influence their toxicity.
There was a significant positive effect in the case of the mixture of AA with
AAgNPs and GA with GAAgNPs. The results obtained suggest that the presence of
stabilizing agents adsorbed on the surface of AgNPs is the main factor in shaping
their toxicity. Nevertheless, the toxic effect can be also tuned by the
introduction of free antioxidant molecules to the AgNP suspensions. © 2021 John
Wiley & Sons, Ltd.
AU - Barbasz, A.
AU - Oćwieja, M.
AU - Piergies, N.
AU - Duraczyńska, D.
AU - Nowak, A.
DB - Scopus
DO - 10.1002/jat.4173
IS - 11
KW - antioxidants
ascorbic acid
cytotoxicity
gallic acid
HL-60
tumoral cells
U-937
Antioxidants
HL-60 Cells
Humans
Metal Nanoparticles
Microscopy, Atomic Force
Silver
Spectrum Analysis, Raman
U937 Cells
antioxidant
caspase 9
interleukin 6
nitric oxide
silver nanoparticle
stabilizing agent
metal nanoparticle
silver
adsorption
apoptosis
Article
biological activity
cell viability
controlled study
cytokine release
dispersity
drug cytotoxicity
drug structure
drug synthesis
electrophoretic mobility
histiocytic lymphoma
HL-60 cell line
human
human cell
inflammation
mitochondrion
MTT assay
particle size
pyroptosis
surface charge
surface enhanced Raman spectroscopy
surface property
suspension
U-937 cell line
voltammetry
X ray photoemission spectroscopy
chemistry
metabolism
Raman spectrometry
ultrastructure
M3 - Article
PY - 2021
SP - 1863-1878
ST - Antioxidant-modulated cytotoxicity of silver nanoparticles
T2 - Journal of Applied Toxicology
TI - Antioxidant-modulated cytotoxicity of silver nanoparticles
85104670839&doi=10.1002%2fjat.4173&partnerID=40&md5=be7cdf006bdb4f11501bcebbdfa46a0
c
VL - 41
ID - 5248
ER -
TY - JOUR
AB - The properties of silver nanoparticles (AgNPs) synthesized using compounds
exhibiting biological activity seem to constitute an interesting issue worthy of
examination. In these studies, two types of AgNPs were synthesized by a chemical
reduction method using well-known antioxidants: gallic acid (GA) and ascorbic acid
(AA). Transmission electron microscopy (TEM) and atomic force microscopy (AFM)
revealed that the AgNPs were spherical. The average size was equal to 26 +/- 6 nm
and 20 +/- 7 nm in the case of ascorbic acid-silver nanoparticles (AAgNPs) and
gallic acid-silver nanoparticles (GAAgNPs), respectively. Surface-enhanced Raman
spectroscopy (SERS) confirmed that the AgNPs were not stabilized by pure forms of
applied antioxidants. Changes in mitochondrial activity and secretion of
inflammatory and apoptosis mediators after the exposure of human promyelocytic (HL-
60) and histiocytic lymphoma (U-937) cells to the AgNPs were studied to determine
the impact of stabilizing layers on nanoparticle toxicity. The GAAgNPs were found
to be more toxic for the cells than the AAgNPs. Their toxicity was manifested by a
strong reduction in mitochondrial activity and induction of the secretion of
interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and caspase-9. The
addition of pure antioxidants to the AgNP suspensions was found to influence their
toxicity. There was a significant positive effect in the case of the mixture of AA
with AAgNPs and GA with GAAgNPs. The results obtained suggest that the presence of
stabilizing agents adsorbed on the surface of AgNPs is the main factor in shaping
their toxicity. Nevertheless, the toxic effect can be also tuned by the
introduction of free antioxidant molecules to the AgNP suspensions.
AN - WOS:000641765900001
AU - Barbasz, A.
AU - Ocwieja, M.
AU - Piergies, N.
AU - Duraczynska, D.
AU - Nowak, A.
C6 - APR 2021
DA - NOV
DO - 10.1002/jat.4173
IS - 11
PY - 2021
SN - 0260-437X
1099-1263
SP - 1863-1878
ST - Antioxidant-modulated cytotoxicity of silver nanoparticles
T2 - JOURNAL OF APPLIED TOXICOLOGY
TI - Antioxidant-modulated cytotoxicity of silver nanoparticles
VL - 41
ID - 5849
ER -
TY - JOUR
AB - Copper(II) and silver(I) hyaluronane-based hydrogel complexes were
synthesised. The amount of metal ion uptaken by hydrogel was determined by atomic
absorption and the hydrogel-metal ion complexes were characterised by water-uptake
measurements, SEM-EDAX and FT-IR analysis. The coordination sites were identified
for both metal ions and the stability at various pH levels was determined. The
cytotoxicity of hydrogel metal ions was evaluated by using fibroblast (3T3) cells.
The copper(II) complex was "in vivo" tested and showed proangiogenic activity,
stimulating the growth of new vessels without inducing an inflammatory reaction.
The antibacterial activity of a silver complex was determined, showing that the
presence of silver ions drastically reduced the adhesion and proliferation of
Staphylococcus epidermidis.
AU - Barbucci, R.
AU - Leone, G.
AU - Magnani, A.
AU - Montanaro, L.
AU - Arciola, C. R.
AU - Peluso, G.
AU - Petillo, O.
DB - Scopus
DO - 10.1039/b205320a
IS - 10
KW - copper complex
hyaluronic acid
metal complex
metal ion
silver derivative
angiogenesis
animal cell
article
atomic absorption
complex formation
cytotoxicity
fibroblast
hydrogel
inflammation
mouse
nonhuman
pH
Staphylococcus epidermidis
synthesis
X ray microanalysis
M3 - Article
PY - 2002
SP - 3084-3092
ST - Cu2+- and Ag+-complexes with a hyaluronane-based hydrogel
T2 - Journal of Materials Chemistry
TI - Cu2+- and Ag+-complexes with a hyaluronane-based hydrogel
0036794482&doi=10.1039%2fb205320a&partnerID=40&md5=26c1218579fd16946180dc16128b0558
VL - 12
ID - 5807
ER -
TY - JOUR
AB - In recent decades, wound dressings have evolved from the simple gauze to
sophisticated, carefully designed functional materials which can enhance wound
healing and eliminate bacterial infections endangering the wounded area. With an
increasing number of multidrug-resistant strains, bacterial overgrowth of the
injured area poses a serious risk that can lead to severe conditions. Nanoparticles
can exhibit remarkable antibacterial properties thus incorporating them into
biocompatible matrices, effective antibacterial wound dressings can be fabricated.
Utilizing electrospinning, nanoparticles could be easily incorporated into fibrous
polymer meshes. Next to nanoparticles, electrospinning allows the simultaneous
encapsulation of small molecules as well, resulting in complex nanocomposite meshes
exhibiting antibacterial and anti-inflammatory and/or analgesic properties. In the
manuscript, we present a one-pot method for the synthesis of silver nanoparticles
(AgNP) in the presence of polysuccinimide (PSI) and a small molecule drug
(paracetamol), followed by the fabrication of an antibacterial wound dressing
system. Thorough characterization of both the AgNP-containing colloidal system and
the meshes were performed. Results reveal the stabilizing effect of PSI and
paracetamol enhancing the formation of a monodisperse colloidal system. Mechanical
studies confirm the reinforcing effect of silver-nanoparticles, and antibacterial
evaluation proves the applicability of the meshes. Drug-release measurement shows
prolonged three-step release kinetics. © 2020 The Authors
AU - Barczikai, D.
AU - Kacsari, V.
AU - Domokos, J.
AU - Szabó, D.
AU - Jedlovszky-Hajdu, A.
C7 - 114575
DB - Scopus
DO - 10.1016/j.molliq.2020.114575
KW - Antibacterial study
Drug release
Electrospinning
Nanocomposite
Nanoparticles
Paracetamol
Wound dressing
Aromatic compounds
Biocompatibility
Colloids
Controlled drug delivery
Drug products
Functional materials
Metal nanoparticles
Molecules
Targeted drug delivery
Tissue regeneration
Anti-inflammatories
Bacterial infections
Multidrug resistants
Reinforcing effects
Small-molecule drugs
Stabilizing effects
M3 - Article
PY - 2021
ST - Interaction of silver nanoparticle and commonly used anti-inflammatory drug
within a poly(amino acid) derivative fibrous mesh
T2 - Journal of Molecular Liquids
TI - Interaction of silver nanoparticle and commonly used anti-inflammatory drug
within a poly(amino acid) derivative fibrous mesh
85094614203&doi=10.1016%2fj.molliq.2020.114575&partnerID=40&md5=313863a9485cd73e298
29c2aa1c40f5a
VL - 322
ID - 5172
ER -
TY - JOUR
AB - The presence of inflammation and demyelination in a central nervous system
(CNS) biopsy points towards a limited, yet heterogeneous group of pathologies, of
which multiple sclerosis (MS) represents one of the principal considerations.
Inflammatory demyelination has also been reported in patients with clinically
suspected primary central nervous system lymphoma (PCNSL), especially when steroids
had been administered prior to biopsy acquisition. The histopathological changes
induced by corticosteroid treatment can range from mild reduction to complete
disappearance of lymphoma cells. It has been proposed that in the absence of
neoplastic B cells, these biopsies are indistinguishable from MS, yet despite the
clinical relevance, no histological studies have specifically compared the two
entities. In this work, we analyzed CNS biopsies from eight patients with
inflammatory demyelination in whom PCNSL was later histologically confirmed, and
compared them with nine well defined early active multiple sclerosis lesions. In
the patients with steroid-treated PCNSL (ST-PCNSL) the interval between first and
second biopsy ranged from 3 to 32 weeks; all of the patients had received
corticosteroids before the first, but not the second biopsy. ST-PCNSL patients were
older than MS patients (mean age: ST-PCNSL: 62 ± 4 years, MS: 30 ± 2 years), and
histological analysis revealed numerous apoptoses, patchy and incomplete rather
than confluent and complete demyelination and a fuzzy lesion edge. The loss of
Luxol fast blue histochemistry was more profound than that of myelin proteins in
immunohistochemistry, and T cell infiltration in ST-PCNSL exceeded that in MS by
around fivefold (P = 0.005). Our data indicate that in the presence of extensive
inflammation and incomplete, inhomogeneous demyelination, the neuropathologist
should refrain from primarily considering autoimmune inflammatory demyelination
and, even in the absence of lymphoma cells, instigate close clinical follow-up of
the patient to detect recurrent lymphoma. © 2017 International Society of
Neuropathology
AU - Barrantes-Freer, A.
AU - Engel, A. S.
AU - Rodríguez-Villagra, O. A.
AU - Winkler, A.
AU - Bergmann, M.
AU - Mawrin, C.
AU - Kuempfel, T.
AU - Pellkofer, H.
AU - Metz, I.
AU - Bleckmann, A.
AU - Hernández-Durán, S.
AU - Schippling, S.
AU - Rushing, E. J.
AU - Frank, S.
AU - Glatzel, M.
AU - Matschke, J.
AU - Hartmann, C.
AU - Reifenberger, G.
AU - Müller, W.
AU - Schildhaus, H. U.
AU - Brück, W.
AU - Stadelmann, C.
DB - Scopus
DO - 10.1111/bpa.12496
IS - 2
KW - corticosteroids
demyelination
diffuse large B cell lymphoma
inflammation
multiple sclerosis
Adrenal Cortex Hormones
Aged
Apoptosis
Biopsy
Central Nervous System Neoplasms
Cohort Studies
Diagnosis, Differential
Female
Humans
Inflammation
Lymphoma
Male
Middle Aged
Multiple Sclerosis
Myelin Sheath
T-Lymphocytes
amyloid precursor protein
steroid
corticosteroid
adult
Article
autoimmune disease
autoimmune inflammatory demyelination
B lymphocyte
cancer patient
CD4 CD8 ratio
cell infiltration
clinical article
corticosteroid therapy
differential diagnosis
disease course
female
follow up
human
human cell
human tissue
image analysis
lymphoma cell
male
middle aged
primary central nervous system lymphoma
silver impregnation
tumor biopsy
tumor spheroid
aged
apoptosis
biopsy
central nervous system tumor
cohort analysis
lymphoma
myelin sheath
pathology
T lymphocyte
M3 - Article
PY - 2018
SP - 225-233
ST - Diagnostic red flags: steroid-treated malignant CNS lymphoma mimicking
T2 - Brain Pathology
TI - Diagnostic red flags: steroid-treated malignant CNS lymphoma mimicking
85017381807&doi=10.1111%2fbpa.12496&partnerID=40&md5=9a168cc0b5e29d3c455568f0602824
8d
VL - 28
ID - 5498
ER -
TY - JOUR
AB - In this work, porous FeMn(-xAg) alloys are fabricated through powder
metallurgy methods. The effects of porosity and Ag addition on the microstructure,
biodegradability, magnetic and mechanical properties of the alloys are
investigated. Studies on the cytocompatibility, inflammatory cytokine response and
antibacterial effect are also conducted. The fabricated alloys exhibit a macro- and
nanoporous structure, with uniformly distributed silver particles. The
biodegradability tests reveal that the release of Mn to the Hank's solution is
higher than that of Fe, without significant differences between the alloys. The
degradation products consist mainly of Fe, Mn, O and compounds enriched in Ca, P
and Cl. As-sintered alloys show a low saturation magnetization value (below 1 emu
g−1), which does not increase significantly with immersion time. The results on
biocompatibility indicate that all tested alloys are non-cytotoxic, but the
addition of Ag might interfere with cell proliferation. However, the ions released
by the FeMn(-xAg) alloys do not induce an inflammatory response in macrophages. The
obtained results on microbiological interactions reveal that although no
significant bactericidal effect is observed at 4 h between FeMn control and FeMn-
5Ag, a significant reduction in the total biofilm biomass of both live and dead
bacteria is observed after 24 h in Ag containing FeMn-5Ag surfaces. © 2023 RSC.
AU - Bartkowska, A.
AU - Careta, O.
AU - Turner, A. B.
AU - Blanquer, A.
AU - Ibañez, E.
AU - Trobos, M.
AU - Nogués, C.
AU - Pellicer, E.
AU - Sort, J.
DB - Scopus
DO - 10.1039/d2ma00867j
IS - 2
M3 - Article
PY - 2022
ST - Biodegradable porous FeMn(-xAg) alloys: assessment of cytocompatibility,
mechanical, magnetic and antibiofilm properties
T2 - Materials Advances
TI - Biodegradable porous FeMn(-xAg) alloys: assessment of cytocompatibility,
85144160899&doi=10.1039%2fd2ma00867j&partnerID=40&md5=9dc1f7c60075600a56c7a8af400b3
d0a
VL - 4
ID - 4989
ER -
TY - JOUR
AB - In this work, porous FeMn(-xAg) alloys are fabricated through powder
metallurgy methods. The effects of porosity and Ag addition on the microstructure,
biodegradability, magnetic and mechanical properties of the alloys are
investigated. Studies on the cytocompatibility, inflammatory cytokine response and
antibacterial effect are also conducted. The fabricated alloys exhibit a macro- and
nanoporous structure, with uniformly distributed silver particles. The
biodegradability tests reveal that the release of Mn to the Hank's solution is
higher than that of Fe, without significant differences between the alloys. The
degradation products consist mainly of Fe, Mn, O and compounds enriched in Ca, P
and Cl. As-sintered alloys show a low saturation magnetization value (below 1 emu
g(-1)), which does not increase significantly with immersion time. The results on
biocompatibility indicate that all tested alloys are non-cytotoxic, but the
addition of Ag might interfere with cell proliferation. However, the ions released
by the FeMn(-xAg) alloys do not induce an inflammatory response in macrophages. The
obtained results on microbiological interactions reveal that although no
significant bactericidal effect is observed at 4 h between FeMn control and FeMn-
5Ag, a significant reduction in the total biofilm biomass of both live and dead
bacteria is observed after 24 h in Ag containing FeMn-5Ag surfaces.
AN - WOS:000897896600001
AU - Bartkowska, A.
AU - Careta, O.
AU - Turner, A. B.
AU - Blanquer, A.
AU - Ibanez, E.
AU - Trobos, M.
AU - Nogues, C.
AU - Pellicer, E.
AU - Sort, J.
C6 - DEC 2022
DA - JAN 23
DO - 10.1039/d2ma00867j
IS - 2
PY - 2023
SN - 2633-5409
SP - 616-630
ST - Biodegradable porous FeMn(-xAg) alloys: assessment of cytocompatibility,
T2 - MATERIALS ADVANCES
TI - Biodegradable porous FeMn(-xAg) alloys: assessment of cytocompatibility,
VL - 4
ID - 6655
ER -
TY - JOUR
AB - Skin explants maintained in culture may represent a reliable model for in
vitro tests of the irritancy of chemicals. During the process of skin irritation
intracellular enzymes migrate into the culture medium. The amount of released
enzyme activity corresponds to the degree of skin damage. Skin of hairless mice
(hr/hr) has been found to be especially useful for this model. Histomorphology
demonstrated that the explants were almost identical to the in vivo situation. Skin
explants of hairless mice of 50 mm2 were used for the tests. The dermal side of the
skin is in contact with the medium whereas the substance is applied to the
epidermal side and incubated for 24 hr. As parameters for the membrane-damaging
effect, the enzymes lactate dehydrogenase and glutamic-oxaloacetate transaminase
were measured. The determination of the glucose utilization during the incubation
period gave additional information about the viability of the cultured skin.
Various chemicals were used. Histological examination complemented the biochemical
results and differentiated epidermal lesions, but was limited by the absence of
inflammatory reactions of the dermal part of the skin. Overall, in vitro skin
culture tests seem to be useful as screening tests prior to in vivo studies and for
the development of new formulations. © 1990.
AU - Bartnik, F. G.
AU - Pittermann, W. F.
AU - Mendorf, N.
AU - Tillman, U.
AU - Künstler, K.
DB - Scopus
DO - 10.1016/0887-2333(90)90067-4
IS - 4-5
KW - Animalia
alpha tocopherol
castor oil
dodecyl sulfate
phosphonic acid derivative
silver nitrate
sodium hydroxide
sulfuric acid
surfactant
animal cell
biological model
conference paper
female
histology
in vitro study
methodology
mouse
nonhuman
organ culture
priority journal
skin irritation
toxicity testing
M3 - Article
PY - 1990
SP - 293-301
ST - Skin organ culture for the study of skin irritancy
TI - Skin organ culture for the study of skin irritancy
0024990485&doi=10.1016%2f0887-2333%2890%2990067-
4&partnerID=40&md5=68012ef87f408ee537b480eabc28b592
VL - 4
ID - 5813
ER -
TY - CONF
AB - Aloevera is one of the oldest medicinal plants and it possesses different
types of pharmacological properties including anti-microbial, anti-inflammatory,
anti-cancer and antioxidant activity. More over Aloevera contains 75 bioactive
compounds such as polyphenol, flavonoids, alkaloids, anthraquinone etc. Such
bioactive compounds can be used to reduce silver ions to produce silver
nanoparticles. In this study, we are trying to formulate bio-synthesised silver
nanoparticle (b-AgNps) by green chemistry approach. It is a very simple, efficient
and eco-friendly approach for silver nanoparticle synthesis that is formed by
reduction of silver nitrate (AgNO3) solution using Aloe vera leaf extract as a
reducing agent. Hydrothermal method was used to prepare b-AgNps using aloe vera
leaf extract as both reducing and stabilizing agent. Then b-AgNps were
characterized by FESEM, XRD, DLS and UV-VIS spectroscopy. Biological activity of b-
AgNps were evaluated by performing cytotoxic test (MTT assay) against breast cancer
cell lines and Fibroblast normal cell lines as well as screening antimicrobial
activity using agar well diffusion method. The results revealed that there was
significant amount of cell growth inhibition against breast cancer cell lines as
well as microbes compare to normal healthy fibroblast cell lines. © Published under
licence by IOP Publishing Ltd.
AU - Basak, P.
AU - Majumder, R.
AU - Jasu, A.
AU - Paul, S.
AU - Biswas, S.
DB - Scopus
DO - 10.1088/1757-899X/410/1/012020
ET - 1
KW - Alovera
bio-synthesised silver nanoparticle (b-AgNps)
Breast cancer cell lines
Reducing agent
Antimicrobial agents
Bioactivity
Cell culture
Cytotoxicity
Diseases
Fibroblasts
Functional materials
Ketones
Metal ions
Metal nanoparticles
Microorganisms
Nitrogen compounds
Plants (botany)
Reducing agents
Ultraviolet visible spectroscopy
Anti-microbial activity
Anti-oxidant activities
Breast cancer cells
Green chemistry approaches
Pharmacological properties
Reduction of silver nitrates
Synthesised
Silver compounds
PY - 2018
ST - Potential Therapeutic Activity of Bio-Synthesized Silver Nanoparticles as
Anticancer and Antimicrobial Agent
T2 - IOP Conference Series: Materials Science and Engineering
TI - Potential Therapeutic Activity of Bio-Synthesized Silver Nanoparticles as
Anticancer and Antimicrobial Agent
85054213504&doi=10.1088%2f1757-899X
%2f410%2f1%2f012020&partnerID=40&md5=3be0fa234f62b129da9e04445fc75fe2
VL - 410
ID - 5419
ER -
TY - JOUR
AB - Wound and its treatment is one of the major health concerns throughout the
globe. Various extrinsic and intrinsic factors can influence the dynamics of
healing mechanism. One such extrinsic factor is moist environment in wound healing.
The advantages of optimum hydration in wound healing are enhanced autolytic
debridement, angiogenesis and accelerated cell proliferation and collagen
formation. But hydrated wounds often end up with patient's uncomfortability,
associated infection, and tissue lipid peroxidation. Healing process prefers
antimicrobial, anti-inflammatory and optimum moist microenvironment. Here, we have
synthesized fumaric acid incorporated agar-silver hydrogel (AA-Ag-FA);
characterized by UV–Visible spectroscopy, FTIR spectroscopy and TEM. The surface
morphology is evaluated through SEM. The size of the silver nanoparticles (Ag NPs)
was found to be 10–15 nm. The hydrogel shows potential antibacterial effect against
Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa which are
predominantly responsible for wound infection. The gel shows reasonable antioxidant
property evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Topical
application of the gel on the wound site heals the wound at much faster rate even
compared to standard (Mega heal, Composition: Colloidal silver 32 ppm hydrogel)
gel. Histological analysis reveals better tissue proliferation (i.e.
epithelialization), more granulation tissue formation, neovascularisation,
fibroblast and mature collagen bundles. The lipid peroxidation of wound tissue
estimated through malondialdehyde (MDA) assay was found to be reasonably less when
treated with AA-Ag-FA hydrogel compared to standard (Mega heal). Cytotoxicity of
the samples tested through MTT assay and live-dead cell staining shows its nontoxic
biocompatibility nature. In our hydrogel scaffold, the bio-degradable agar-agar
provides the moist environment; the Ag NPs inside the gel acts as bactericidal
agent and fumaric acid facilities the antioxidant and angiogenesis path implicitly.
© 2020 Elsevier B.V.
AU - Basha, S. I.
AU - Ghosh, S.
AU - Vinothkumar, K.
AU - Ramesh, B.
AU - kumari, P. H. P.
AU - Mohan, K. V. M.
AU - Sukumar, E.
C7 - 110743
DB - Scopus
DO - 10.1016/j.msec.2020.110743
KW - Agar-silver hydrogel
Antibacterial
Antioxidant
Collagen-growth-factor
Wound healing
Agar
Animals
Antioxidants
Apoptosis
Cell Line
Fumarates
Humans
Hydrogels
Male
Metal Nanoparticles
Mice
Neovascularization, Physiologic
Rats
Rats, Wistar
Silver
Wound Healing
Algae
Ascorbic acid
Biocompatibility
Cell culture
Cell proliferation
Collagen
Escherichia coli
Hydration
Lipids
Morphology
Oxidation
Polysaccharides
Surface morphology
Tissue
Tissue regeneration
agar
antiinfective agent
antioxidant
biomaterial
fumaric acid
fumaric acid derivative
metal nanoparticle
silver
2 ,2-diphenyl-1-picrylhydrazyl
Antioxidant properties
Growth factor
Histological analysis
angiogenesis
animal
apoptosis
cell line
chemistry
drug effect
human
hydrogel
male
metabolism
mouse
pharmacology
rat
Wistar rat
wound healing
M3 - Article
PY - 2020
ST - Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional
avenue to tackle wound healing
T2 - Materials Science and Engineering C
TI - Fumaric acid incorporated Ag/agar-agar hybrid hydrogel: A multifunctional
avenue to tackle wound healing
85079846126&doi=10.1016%2fj.msec.2020.110743&partnerID=40&md5=6fc7db033f6ddb09630aa
fc2ef472ca8
VL - 111
ID - 5300
ER -
TY - JOUR
AB - Background: Nanopharmaceuticals have rapidly emerged as a means to cure
several diseases. There are numerous reports describing the development and
application of nanopharmaceuticals. Here, we discussed nanoparticle synthesis and
the mechanisms to scavenge free radicals. We also discuss their major properties
and list several commercially available nanomedicines. Results: Reactive oxygen and
hydrogen species are formed during normal metabolism, and excessive reactive
species can damage proteins, lipids, and DNA and cause disease. Plant-and microbe-
based nanoparticles, which can protect tissues from free radical damage, have
recently gained research momentum because they are inexpensive and safe.
Conclusion: Synthetic and biocompatible nanoparticles exhibit antioxidant,
antidiabetic, anti-inflammatory, and anticancer properties, which can be used to
treat several diseases. Further studies are needed to investigate their sizes,
dose-dependent activities, and mechanisms of action. © 2019 Bentham Science
Publishers.
AU - Baskaran, X. R.
AU - Vigila, A. V. G.
AU - Rajan, K.
AU - Zhang, S.
AU - Liao, W.
DB - Scopus
DO - 10.2174/1381612825666190716110330
IS - 24
KW - Anticancer
Antioxidant
DPPH
Free radicals
Free Radical Scavengers
Nanomedicine
Nanoparticles
alginic acid
antibiofim
antidiabetic agent
antiinfective agent
antioxidant
calcium phosphate
copper nanoparticle
curcumin
cytotoxic agent
free radical
gold nanoparticle
hydrogel
hydrogen
lactoferrin
lipid
metal nanoparticle
nanocarrier
nanoparticle
orotic acid
protein
silica nanoparticle
silver nanoparticle
tin oxide
unclassified drug
unindexed drug
ascorbic acid
Azadirachta indica extract
butylated hydroxyanisole
butylcresol
chitosan nanoparticle
cichoric acid
citrate sodium
gallic acid propyl ester
genistein
ginseng extract
herbaceous agent
hydrogen peroxide
hydroxyl radical
Moringa oleifera extract
peppermint oil
superoxide
tert butylhydroquinone
thymol
Thymus vulgaris extract
scavenger
biocompatibility
biosynthesis
diseases
DNA damage
dose response
drug approval
drug cost
drug mechanism
drug research
drug safety
free radical scavenging
human
metabolism
microorganism
nanoemulsion
nanomedicine
nonhuman
oxidative stress
particle size
plant
priority journal
Review
drug cytotoxicity
IC50
powder
Rhus coriaria
M3 - Review
PY - 2019
SP - 2677-2693
ST - Free radical scavenging and some pharmaceutical utilities of nanoparticles in
the recent scenario
T2 - Current Pharmaceutical Design
TI - Free radical scavenging and some pharmaceutical utilities of nanoparticles in
the recent scenario
85073124344&doi=10.2174%2f1381612825666190716110330&partnerID=40&md5=6d4004a8f040d7
a9882d5609a693d6c4
VL - 25
ID - 5384
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are among the most commonly used engineered NPs
and various commercially available products are designed to come in direct contact
with the skin (wound dressings, textiles, creams, among others). Currently, there
is limited understanding of the influence of coatings on the toxicity of AgNPs and
in particular their ability to impact on AgNP's mediated inflammatory responses. As
AgNPs are often stabilized by different coatings, including citrate and
polyethyleneglycol (PEG), in this study we investigate the influence of citrate
(Cit10) or PEG (PEG10) coatings to 10 nm AgNP on skin, using human HaCaT
keratinocytes. AgNPs cytotoxicity and inflammatory response (nuclear factor (NF)-
kappa B induction and cytokine production) of HaCaT were assessed after in vitro
exposure to 10 and 40 A mu g/mL after 4, 24, and 48 h. Results showed that although
both types of coated AgNPs decreased cell proliferation and viability, Cit10 AgNPs
were more toxic. NF-kappa B inhibition was observed for the highest concentration
(40 A mu g/mL) of PEG10 AgNPs, and the putative link to early apoptotic pathways
observed in these cells is discussed. No production of IL-1 beta, IL-6, IL-10, and
TNF alpha was stimulated by AgNPs. Furthermore, Cit10 and PEG10 AgNPs decreased the
release of MCP-1 by HaCaT cells after 48 h of exposure. As cytokines are vital for
the immunologic regulation in the human body, and it is demonstrated that they may
interfere with NPs, more research is needed to understand how different AgNPs
affect the immune system.
AN - WOS:000380274500001
AU - Bastos, V.
AU - Brown, D.
AU - Johnston, H.
AU - Daniel-da-Silva, A. L.
AU - Duarte, I. F.
AU - Santos, C.
AU - Oliveira, H.
C7 - 205
DA - JUL 20
DO - 10.1007/s11051-016-3515-x
IS - 7
PY - 2016
SN - 1388-0764
1572-896X
ST - Inflammatory responses of a human keratinocyte cell line to 10 nm citrate-
and PEG-coated silver nanoparticles
TI - Inflammatory responses of a human keratinocyte cell line to 10 nm citrate-
VL - 18
ID - 5964
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are among the most commonly used engineered NPs
and various commercially available products are designed to come in direct contact
with the skin (wound dressings, textiles, creams, among others). Currently, there
is limited understanding of the influence of coatings on the toxicity of AgNPs and
in particular their ability to impact on AgNP’s mediated inflammatory responses. As
AgNPs are often stabilized by different coatings, including citrate and
polyethyleneglycol (PEG), in this study we investigate the influence of citrate
(Cit10) or PEG (PEG10) coatings to 10 nm AgNP on skin, using human HaCaT
keratinocytes. AgNPs cytotoxicity and inflammatory response (nuclear factor (NF)-κB
induction and cytokine production) of HaCaT were assessed after in vitro exposure
to 10 and 40 µg/mL after 4, 24, and 48 h. Results showed that although both types
of coated AgNPs decreased cell proliferation and viability, Cit10 AgNPs were more
toxic. NF-κB inhibition was observed for the highest concentration (40 µg/mL) of
PEG10 AgNPs, and the putative link to early apoptotic pathways observed in these
cells is discussed. No production of IL-1β, IL-6, IL-10, and TNFα was stimulated by
AgNPs. Furthermore, Cit10 and PEG10 AgNPs decreased the release of MCP-1 by HaCaT
cells after 48 h of exposure. As cytokines are vital for the immunologic regulation
in the human body, and it is demonstrated that they may interfere with NPs, more
research is needed to understand how different AgNPs affect the immune system. ©
2016, Springer Science+Business Media Dordrecht.
AU - Bastos, V.
AU - Brown, D.
AU - Johnston, H.
AU - Duarte, I. F.
AU - Santos, C.
AU - Oliveira, H.
C7 - 205
DB - Scopus
DO - 10.1007/s11051-016-3515-x
IS - 7
KW - Citrate-AgNPs
Environmental and health effects
HaCaT cells
Inflammatory responses
Nanotoxicology
PEG-AgNPs
Skin
Cell culture
Cell proliferation
Cells
Coatings
Cytology
Metal nanoparticles
Nanoparticles
citric acid
interleukin 10
interleukin 1beta
interleukin 6
macrogol
silver nanoparticle
Apoptotic pathways
Cytokine production
apoptosis
Article
cell growth
cell proliferation
cell viability
controlled study
cytokine production
cytokine release
drug cytotoxicity
human
human cell
in vitro study
inflammation
keratinocyte
nanotoxicology
priority journal
Silver
M3 - Article
PY - 2016
ST - Inflammatory responses of a human keratinocyte cell line to 10 nm citrate-
TI - Inflammatory responses of a human keratinocyte cell line to 10 nm citrate-
84983086768&doi=10.1007%2fs11051-016-3515-
x&partnerID=40&md5=c6990ccd3f71a6a432125a9d6367bd20
VL - 18
ID - 5515
ER -
TY - JOUR
AB - Introduction: Periodontitis is a chronic inflammatory disease, resulting due
to host immune response against subgingival biofilm. Most conventional treatment
protocols aim to control the subgingival biofilm by mechanical means, such as
dental scaling and root planning, and frequently accompanied by antibacterial co-
adjuvant therapies, including antibiotics, antiseptics, or probiotics. Local drug
delivery facilitates administration of a lower dose of the drug to the target site,
but at higher concentration, thereby reducing systemic adverse effects and
toxicity. The present systematic review was conducted with the aim of identifying
and reporting nanoparticle based periodontal drug delivery systems, with a specific
focus on current trends and future perspectives in this field. Materials & methods:
Comprehensive literature search, restricted to published reports in English
language between January 2000 and February 2022, was done electronically and
manually. Search queries were addressed to the following electronic databases
including, PubMed (MEDLINE), Science Direct (Elsevier), Cochrane Library, Web of
Science (Clarivate Analytics) and Scholar (Google). Database search returned 780
results which were screened based on title, author names and publication dates, to
identify 13 studies fulfilling the review criteria. Results: Data from the 13
included studies were reviewed and tabulated, elaborating the type of nanoparticle
used, drug delivered and tissues/cells/subcellular components targeted by
periodontal drug delivery. While majority of the studies were conducted in vitro,
there were 3 in vivo studies and 3 clinical studies. Using nanotechnology for drug
delivery resulted in better inhibition of bacterial growth, inflammatory modulation
favoring resolution of periodontitis and capability for early tissue regeneration.
Conclusion: Recent developments in nanotechnology have enabled targeted local
delivery of drugs and anti-inflammatory biomolecules, in synergy with
nanoparticles, towards periodontal pathogens, inflammatory cells and periodontal
tissues. Further research evaluating clinical periodontal disease management
through nanoparticle based local drug delivery drugs is highly recommended. © 2022
The Authors
AU - Basudan, A. M.
DB - Scopus
DO - 10.1016/j.sdentj.2022.09.006
IS - 8
KW - Local drug delivery
Nanoparticles
Nanotechnology
Periodontal diseases
azithromycin
clarithromycin
doxycycline
drug
gelatin
glutaraldehyde
gold nanoparticle
metronidazole
mevinolin
minocycline
nanocarrier
placebo
polyglactin
silicon dioxide
silver nanoparticle
Syzygium cumini extract
tetracycline
zinc
antibiotic therapy
bacterial growth
Cochrane Library
controlled study
flower
human
Medline
nanoencapsulation
nanomedicine
nonhuman
periodontal drug administration
periodontitis
plant seed
Review
ScienceDirect
search engine
systematic review
Syzygium cumini
tissue regeneration
Web of Science
M3 - Review
PY - 2022
SP - 669-680
ST - Nanoparticle based periodontal drug delivery – A review on current trends and
future perspectives
T2 - Saudi Dental Journal
TI - Nanoparticle based periodontal drug delivery – A review on current trends and
future perspectives
85144245826&doi=10.1016%2fj.sdentj.2022.09.006&partnerID=40&md5=7658392a9d953fa45aa
cdea813052df9
VL - 34
ID - 4979
ER -
TY - JOUR
AB - Malva sylvestris is a plant commonly found in Europe, Asia, and Africa. The
leaves and flowers of this plant have been used for centuries in traditional
medicine to treat various ailments such as cough, cold, diarrhoea, and
constipation. Google Scholar, PubMed, Scopus, and Web of Science were used to
search for relevant material on the phytochemical profiling and pharmacologic
activities of Malva sylvestris. The techniques used in phytochemical profiling and
the pharmacologic activity of each compound were extracted from the included
studies, including in vitro, in vivo, and clinical studies. The phytochemical
analysis of Malva sylvestris revealed that the leaves and flowers are the most
commonly used parts of the plant and contain various bioactive compounds such as
flavonoids, mucilages, terpenoids, phenol derivatives, coumarins, sterols, tannins,
saponins, and alkaloids. These phytochemicals are responsible for the many
pharmacological activities of Malva sylvestris, such as anti-inflammatory,
antimicrobial, hepatoprotective, laxative, antiproliferative and antioxidant
properties. This review has presented an overview of the antinociceptive and anti-
inflammatory activities and the cytotoxic effects of Malva sylvestris on different
types of cancer cells. It has also summarised the work on developing copper oxide
nanoparticles using Malva sylvestris leaf extract and its potential use in food and
medicine. This review aims to highlight the traditional uses, phytochemistry,
pharmacological activities, and safety of Malva sylvestris. © 2022, The Author(s).
AU - Batiha, G. E. S.
AU - Tene, S. T.
AU - Teibo, J. O.
AU - Shaheen, H. M.
AU - Oluwatoba, O. S.
AU - Teibo, T. K. A.
AU - Al-kuraishy, H. M.
AU - Al-Garbee, A. I.
AU - Alexiou, A.
AU - Papadakis, M.
DB - Scopus
DO - 10.1007/s00210-022-02329-w
IS - 3
KW - Malva sylvestris
Pharmacological activities
Phytochemistry profiling
Safety
Traditional medicine
Malva
Phytochemicals
Phytotherapy
Plant Extracts
alkaloid
amino acid derivative
antiinfective agent
antinociceptive agent
antioxidant
coumarin derivative
cytotoxic agent
element
enzyme
fatty acid
flavonoid
laxative
liver protective agent
Malva sylvestris extract
metal nanoparticle
phenol derivative
phytosterol
pigment
plant extract
protein derivative
saponin
silver nanoparticle
tannin derivative
terpenoid
unclassified drug
vitamin
phytochemical
acute toxicity
agronomics
alternative medicine
antinociception
cancer cell
chemical analysis
chemical composition
chemical fingerprinting
crop
dietary fiber
drug activity
drug cytotoxicity
drug safety
ecology
economic aspect
flower
green chemistry
human
liver protection
medicinal plant
mucilage
nonhuman
phytochemistry
plant leaf
Review
synthesis
traditional medicine
veterinary medicine
chemistry
phytotherapy
M3 - Review
PY - 2023
SP - 421-440
ST - The phytochemical profiling, pharmacological activities, and safety of malva
sylvestris: a review
T2 - Naunyn-Schmiedeberg's Archives of Pharmacology
TI - The phytochemical profiling, pharmacological activities, and safety of malva
sylvestris: a review
85142434570&doi=10.1007%2fs00210-022-02329-
w&partnerID=40&md5=a13f78eeb2764975922b68bf318ba127
VL - 396
ID - 5004
ER -
TY - JOUR
AB - Objective: To compare the rate of cell proliferation and expression of
antiapoptotic protein Bcl-2 between drug-induced gingival overgrowth (DIGO) and
clinical healthy gingiva (CHG) and to establish associations with histopathological
features. Material and Methods: Twenty specimens of DIGO and 20 CHG specimens were
submitted to morphological and immunohistochemical analysis by light microscopy.
Cell proliferation (Ki-67) and the expression of Bcl-2 were evaluated in epithelial
cells and spindle-shaped mononuclear cells of the connective tissue by establishing
the labeling index (LI). Results: In epithelial tissue, the mean LI for Ki-67 was
17.2% in DIGO and 21.71% in CHG (p = 0.137). The mean LIs for Bcl-2 in epithelial
tissue were 14.67% and 10.24% in DIGO and CHG, respectively (p = 0.026). In
connective tissue, DIGO and CHG specimens exhibited low LIs for Ki-67 and Bcl-2,
with mean values of less than 0.5% in both groups. No significant differences in
the LIs for Ki-67 or Bcl-2 in epithelial tissue were observed according to the
degree of collagenization, degree of vascularization and intensity of inflammatory
infiltration (p > 0.05). No significant correlations were observed between the LIs
for Ki-67 and Bcl-2 (p > 0.05). Conclusion: The present results suggest that the
pathogenesis of DIGO does not involve increased proliferation or decreased
apoptosis of fibroblasts. On the other hand, the morphological pattern of elongated
epithelial cristae observed in DIGO could mainly be due to the inhibition of
keratinocyte apoptosis and not to increased proliferation of these cells.
AD - Batista, Ana Luzia Araújo
s.af
Mendonça, Angélica Kercya Pereira de
s.af
Freitas, Roseana de Almeida
Federal University of Rio Grande do Norte. Natal. BR
Alves, Pollianna Muniz
State University of Paraíba. Campina Grande. BR
Godoy, Gustavo Pina
Federal University of Pernambuco. Recife. BR
Nonaka, Cassiano Francisco Weege
State University of Paraíba. Campina Grande. BR
Lins, Ruthinéia Diógenes Alves Uchôa
Federal University of Rio Grande do Norte. Natal. BR
AU - Batista, Ana Luzia Araújo
AU - Mendonça, Angélica Kercya Pereira de
AU - Freitas, Roseana de Almeida
AU - Alves, Pollianna Muniz
AU - Godoy, Gustavo Pina
AU - Nonaka, Cassiano Francisco Weege
AU - Lins, Ruthinéia Diógenes Alves Uchôa
C1 - 20180921
DA - 2017/01
DB - LILACS
DO - 10.4034/PBOCI.2017.171.08
IS - 1
KW - Genes
Gingival Overgrowth
Ki-67 Antigen
bcl-2
LA - en
PY - 2017
SN - 1519-0501
SP - e3331-e3331
ST - Immunohistochemical Analysis of Cell Proliferation and Bcl-2 Expression in
Drug-Induced Gingival Overgrowth
T2 - Pesqui. bras. odontopediatria clín. integr
TI - Immunohistochemical Analysis of Cell Proliferation and Bcl-2 Expression in
Drug-Induced Gingival Overgrowth
UR - http://revista.uepb.edu.br/index.php/pboci/article/view/3331/pdf
UR - http://fi-admin.bvsalud.org/document/view/zhyh8
VL - 17
ID - 4928
ER -
TY - JOUR
AB - The incorporation of antimicrobial substances like silver into implant
surface coatings is one promising concept against primary infections of
endoprosthesis, especially for immunocompromised patients as well as against
reinfection after revision operations. However, besides good antimicrobial and
mechanical properties it is equally important that the implant material does not
disturb the local microvascular perfusion of muscle tissue to enable microbial host
defense and tissue repair processes. In this study the biocompatibility of a newly
developed TiAg-composite coating applied on conventional titanium via physical
vapor deposition was analysed. To evaluate the local microvascular and inflammatory
response of striated muscle tissue upon implantation of TiAg-coated plates the
murine dorsal skinfold chamber model was used. We repetitively examined local
capillary and venular perfusion, endothelial integrity as well as leucocyte
activation by intravital fluorescence microscopy at 1 h, 24 h as well as 3 and
7 days after implantation. TiAg-implants were well tolerated by the vascular system
as indicated by intact functional capillary density and endothelial integrity
compared to pure titanium plates and controls without a metal implant. Furthermore,
quantification of rolling and adherent leucocytes did not reveal signs of
inflammation upon TiAg-implantation. © 2015, Springer Science+Business Media New
York.
AU - Behrendt, A. K.
AU - Beythien, M.
AU - Huber, J.
AU - Zufraß, T.
AU - Butschkau, A.
AU - Mittlmeier, T.
AU - Vollmar, B.
DB - Scopus
DO - 10.1007/s10856-014-5373-3
IS - 1
KW - Aluminum
Animals
Bone and Bones
Bone Substitutes
Capillaries
Cell Adhesion
Coated Materials, Biocompatible
Female
Hemodynamics
Inflammation
Leukocyte Rolling
Leukocytes
Male
Mice
Mice, Inbred C57BL
Microcirculation
Microscopy, Fluorescence
Perfusion
Silver
Titanium
Murinae
Biocompatibility
Biomechanics
Blood vessels
Cardiovascular system
Composite coatings
Fluorescence microscopy
Mechanical properties
Microorganisms
Muscle
Physical vapor deposition
Repair
Tissue
silver
titanium
aluminum
antiinfective agent
biomaterial
bone prosthesis
Antimicrobial substance
Capillary density
Dorsal skinfold chambers
Implant materials
Pure titanium plates
Striated muscles
Vascular system
animal experiment
animal tissue
Article
capillary density
controlled study
dorsal skinfold chamber
immune response
in vivo study
inflammation
leukocyte activation
material coating
metal implant
mouse
muscle tissue
nonhuman
priority journal
skeletal muscle
skinfold
animal
bone
C57BL mouse
capillary
cell adhesion
chemistry
cytology
female
hemodynamics
leukocyte
leukocyte rolling
male
metabolism
microcirculation
pathology
perfusion
Neural prostheses
M3 - Article
PY - 2015
SP - 1-11
ST - New TiAg composite coating for bone prosthesis engineering shows promising
microvascular compatibility in the murine dorsal skinfold chamber model
T2 - Journal of Materials Science: Materials in Medicine
TI - New TiAg composite coating for bone prosthesis engineering shows promising
microvascular compatibility in the murine dorsal skinfold chamber model
84983573947&doi=10.1007%2fs10856-014-5373-
3&partnerID=40&md5=2b7af4051a462f90c03c813cca6c598e
VL - 26
ID - 5576
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have wide applications. Production of AgNPs can
be occurred through different method chemical, physical, and green methods. The
most popular methods are chemical approaches. Marine organisms exhibit a wide range
of bioactivity. The present study was designed to establish the biosynthesis of
silver nanoparticles from marine crustacean extract of the hard and soft parts of
male and female E. massavensis. The microstructure, morphology and optical
absorption properties of the nanoparticles were characterized by X-ray diffraction
(XRD), scanning electron microscopy (SEM) and UV-visible spectroscopy. The
formation of silver nanoparticles was confirmed by Uv-Vis absorption and the
spectra were observed plasmon bands between 441.79-462.74 nm. XRD results show that
the nanoparticles are crystalline in nature. SEM images detected the quasi-
spherical AgNPs morphological shape. Silver nanoparticles from marine crustacean
extract of the hard part of male E. massavensis (HM4) showed the best results in
morphology and particle size. Evaluation of the cytotoxicity of AgNPs (HM4) on
different cancer cell lines antiviral, anti-microbial, anti-diabetic, anti-
arthritic, anti-aging and anti-inflammatory properties were assessed. AgNPs
characterization may be introduced a promising applications in medical aspects. ©
2021 National Information and Documentation Center (NIDOC)
AU - Beltagy, D. M.
AU - Abdo, N. I.
AU - Samak, N. M.
AU - El-Khodary, G. M.
AU - Abdel-Aziz, K. K.
AU - Mona, M. H.
DB - Scopus
DO - 10.21608/ejchem.2021.70308.3549
IS - 8
KW - Bioapplications
Biosynthesis
Cytotoxicity
Marine crustacean
SEM
UV-Vis
XRD
M3 - Article
PY - 2021
SP - 4653-4662
ST - Beneficial bioapplications of silver nanoparticles synthesized by a marine
crustacean (Erugosquilla massavensis)
T2 - Egyptian Journal of Chemistry
TI - Beneficial bioapplications of silver nanoparticles synthesized by a marine
crustacean (Erugosquilla massavensis)
85112304638&doi=10.21608%2fejchem.2021.70308.3549&partnerID=40&md5=81e7942f785a5537
497283e0af09313d
VL - 64
ID - 5208
ER -
TY - JOUR
AB - Consumption of ethanol may have severe effects on human organs and tissues
and lead to acute and chronic inflammation of internal organs. The present study
aims at investigating the potential protective effects of three different extracts
prepared from the leaves, root, and stem of the sumac, Rhus tripartita, against
ethanol-induced toxicity and inflammation using intestinal cells as a cell culture
system, in vitro model of the intestinal mucosa. The results showed an induction of
cytotoxicity by ethanol, which was partially reversed by co-administration of the
plant extracts. As part of investigating the cellular response and the mechanism of
toxicity, the role of reduced thiols and glutathione-S-transferases were assessed.
In addition, intestinal cells were artificially imposed to an inflammation state
and the anti-inflammatory effect of the extracts was estimated by determination of
interleukin-8. Finally, a detailed characterization of the contents of the three
plant extracts by high resolution Nuclear Magnetic Resonance (NMR) spectroscopy and
mass spectrometry revealed significant differences in their chemical compositions.
(C) 2017 Elsevier B.V. All rights reserved.
AN - WOS:000423497000044
AU - Ben Barka, Z.
AU - Grintzalis, K.
AU - Polet, M.
AU - Heude, C.
AU - Sommer, U.
AU - Ben Miled, H.
AU - Ben Rhouma, K.
AU - Mohsen, S.
AU - Tebourbi, O.
AU - Schneider, Y. J.
DA - FEB 20
DO - 10.1016/j.jpba.2017.12.032
PY - 2018
SN - 0731-7085
1873-264X
SP - 347-354
ST - A combination of NMR and liquid chromatography to characterize the protective
effects of Rhus tripartita extracts on ethanol-induced toxicity and inflammation on
intestinal cells
T2 - JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
TI - A combination of NMR and liquid chromatography to characterize the protective
effects of Rhus tripartita extracts on ethanol-induced toxicity and inflammation on
intestinal cells
VL - 150
ID - 6065
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been reported as stressors for the
bivalves' immune system at different regulatory levels, impacting the detection
step and receptors, and other mediators, as well as effector molecules. However,
studies on how AgNPs impact the transmission of signals from receptors and whether
they have an effect on mediators and transcription factors are still scarce. This
study aims to investigate the effect of 12 hours of in vivo exposure to 100 mu g/L
of AgNPs on the gene expression of the cytosolic adaptor Myeloid, the
differentiation protein 88 (MgMyD88-b), and the interferon regulatory factor (Me4-
IRF) in the gills and digestive gland of Mytilus galloprovincialis, before and
after blocking two major uptake pathways of nanoparticles (clathrin- and caveolae-
mediated endocytosis). The results illustrate a tissue-specific gene expression of
the MgMyD88-b and the Me4-IRF in the gills and digestive gland of M.
galloprovincialis. In the gills, AgNPs did not significantly impact the expression
of the two genes. However, blocking the caveolae-mediated endocytosis decreased the
expression of Me4-IRF. However, inhibition of clathrin-mediated endocytosis in the
digestive gland recorded a significant decrease in the expression of MgMyD88-b.
Overall, the inhibition of the AgNPs' uptake routes have highlighted their
potential interference with the immune response through the studied mediators'
genes, which need to be studied further in future investigations.
AN - WOS:000670088300001
AU - Ben Younes, R.
AU - Bouallegui, Y.
AU - Fezai, O.
AU - Mezni, A.
AU - Touaylia, S.
AU - Oueslati, R.
C6 - JUL 2021
DA - SEP 3
DO - 10.1080/01480545.2021.1945128
IS - 5
PY - 2022
SN - 0148-0545
1525-6014
SP - 2371-2378
ST - Silver nanoparticles' impact on the gene expression of the cytosolic adaptor
MyD-88 and the interferon regulatory factor IRF in the gills and digestive gland of
mytilus galloprovincialis
T2 - DRUG AND CHEMICAL TOXICOLOGY
TI - Silver nanoparticles' impact on the gene expression of the cytosolic adaptor
MyD-88 and the interferon regulatory factor IRF in the gills and digestive gland of
mytilus galloprovincialis
VL - 45
ID - 6334
ER -
TY - JOUR
AB - Background: Diesel exhaust is carcinogenic and exposure to diesel particles
cause health effects. We investigated the toxicity of diesel exhaust particles
designed to have varying physicochemical properties in order to attribute health
effects to specific particle characteristics. Particles from three fuel types were
compared at 13% engine intake O2 concentration: MK1 ultra low sulfur diesel (DEP13)
and the two renewable diesel fuels hydrotreated vegetable oil (HVO13) and rapeseed
methyl ester (RME13). Additionally, diesel particles from MK1 ultra low sulfur
diesel were generated at 9.7% (DEP9.7) and 17% (DEP17) intake O2 concentration. We
evaluated physicochemical properties and histopathological, inflammatory and
genotoxic responses on day 1, 28, and 90 after single intratracheal instillation in
mice compared to reference diesel particles and carbon black. Results: Moderate
variations were seen in physical properties for the five particles: primary
particle diameter: 15-22 nm, specific surface area: 152-222 m2/g, and count median
mobility diameter: 55-103 nm. Larger differences were found in chemical
composition: organic carbon/total carbon ratio (0.12-0.60), polycyclic aromatic
hydrocarbon content (1-27 μg/mg) and acid-extractable metal content (0.9-16 μg/mg).
Intratracheal exposure to all five particles induced similar toxicological
responses, with different potency. Lung particle retention was observed in DEP13
and HVO13 exposed mice on day 28 post-exposure, with less retention for the other
fuel types. RME exposure induced limited response whereas the remaining particles
induced dose-dependent inflammation and acute phase response on day 1. DEP13
induced acute phase response on day 28 and inflammation on day 90. DNA strand break
levels were not increased as compared to vehicle, but were increased in lung and
liver compared to blank filter extraction control. Neutrophil influx on day 1
correlated best with estimated deposited surface area, but also with elemental
carbon, organic carbon and PAHs. DNA strand break levels in lung on day 28 and in
liver on day 90 correlated with acellular particle-induced ROS. Conclusions: We
studied diesel exhaust particles designed to differ in physicochemical properties.
Our study highlights specific surface area, elemental carbon content, PAHs and ROS-
generating potential as physicochemical predictors of diesel particle toxicity. ©
2020 The Author(s).
AU - Bendtsen, K. M.
AU - Gren, L.
AU - Malmborg, V. B.
AU - Shukla, P. C.
AU - Tunér, M.
AU - Essig, Y. J.
AU - Krais, A. M.
AU - Clausen, P. A.
AU - Berthing, T.
AU - Loeschner, K.
AU - Jacobsen, N. R.
AU - Wolff, H.
AU - Pagels, J.
AU - Vogel, U. B.
C7 - 38
DB - Scopus
DO - 10.1186/s12989-020-00369-9
IS - 1
KW - Diesel exhaust particles - ultrafine particles
Exhaust gas recirculation
Renewable diesel fuels
Toxicity
Animals
Carbon
Carcinogens
DNA Damage
Gasoline
Lung
Mice
Mice, Inbred C57BL
Particulate Matter
Polycyclic Aromatic Hydrocarbons
Vehicle Emissions
arsenic
black carbon
cadmium
carbon monoxide
cesium
chromium
cobalt
copper
diesel fuel
hydrotreated vegetable oil
indium
iron
manganese
nickel
nitrogen oxide
organic carbon
polycyclic aromatic hydrocarbon
rapeseed methyl ester
rapeseed oil
renewable fuel
rubidium
selenium
silver
strontium
sulfur
unclassified drug
vanadium
vegetable oil
volatile organic compound
carbon
carcinogen
gasoline
acute phase response
animal cell
animal experiment
animal tissue
Article
C57BL 6 mouse
concentration (parameter)
controlled study
count median mobility diameter
diesel engine
diesel particulate matter
DNA strand breakage
extraction
female
genotoxicity
histopathology
inflammation
inhalation
liver tissue
lung parenchyma
lung toxicity
mouse
nonhuman
particle size
particulate matter exposure
physical chemistry
physical parameters
priority journal
surface area
toxicity testing
trachea
animal
C57BL mouse
DNA damage
exhaust gas
lung
particulate matter
toxicity
M3 - Article
PY - 2020
ST - Particle characterization and toxicity in C57BL/6 mice following instillation
of five different diesel exhaust particles designed to differ in physicochemical
properties
TI - Particle characterization and toxicity in C57BL/6 mice following instillation
of five different diesel exhaust particles designed to differ in physicochemical
properties
85089261623&doi=10.1186%2fs12989-020-00369-
9&partnerID=40&md5=f65dfd261ca16e0db9d207afca3e15ab
VL - 17
ID - 5290
ER -
TY - JOUR
AB - Purpose: The leaves and flowering stem of Origanum vulgare contain essential
oils, flavonoids, phenolic acids and anthocyanins. We propose a new, simple, one-
pot, O. vulgare extract (OVE) mediated green synthesis method of biocompatible gold
nanoparticles (AuNPs) possessing improved antioxidant, antimicrobial and plasmonic
properties. Materials and methods: Different concentrations of OVEs were used to
reduce gold ions and to synthetize biocompatible spherical AuNPs. Their morphology
and physical properties have been investigated by means of transmission electron
microscopy, ultraviolet-visible absorption spectroscopy, photon correlation
spectroscopy and Fourier transform infrared spectroscopy, whereas their plasmonic
properties have been tested using surface-enhanced Raman spectroscopy (SERS). The
antioxidant properties of nanoparticles (NPs) have been evaluated by 2,2-diphenyl-
1-picrylhydrazyl radical scavenging assay, and the antimicrobial tests were
performed using the disk diffusion assay. Their cytotoxicity has been assessed by
means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: The experimental results confirmed the successful synthesis of
biocompatible, spherical, plasmonic NPs having a mean diameter of similar to 40 nm
and an outstanding aqueous stability. This new class of NPs exhibits a very good
antioxidant activity and presents interesting inhibitory effects against
Staphylococcus aureus and Candida albicans. Due to their plasmonic properties,
AuNPs are used as SERS substrates for the detection of a test molecule (methylene
blue) up to a concentration of 10(-7) M and a pharmaceutical compound (propranolol)
in solution. Cytotoxicity assays revealed that AuNPs are better tolerated by normal
human dermal fibroblast cells, while the melanoma cancer cells are more sensitive.
Conclusion: The biocompatible AuNPs synthetized using OVEs showed significant
bactericidal and antimycotic activities, the most sensitive microorganisms being S.
aureus and C. albicans, both commonly involved in various dermatological
infections. Moreover, the significant antioxidant effect might recommend their use
for protective and/or preventive effect in various skin inflammatory conditions,
including the reduction in side effects in dermatological infections. Meanwhile,
the as-synthesized biocompatible AuNPs can be successfully used as SERS substrates
for the detection of pharmaceutical compounds in aqueous solutions.
AN - WOS:000426467400001
AU - Benedec, D.
AU - Oniga, I.
AU - Cuibus, F.
AU - Sevastre, B.
AU - Stiufiuc, G.
AU - Duma, M.
AU - Hanganu, D.
AU - Iacovita, C.
AU - Stiufiuc, R.
AU - Lucaciu, C. M.
DO - 10.2147/IJN.S149819
PY - 2018
SN - 1178-2013
SP - 1041-1058
ST - Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles
simultaneously possessing plasmonic, antioxidant and antimicrobial properties
TI - Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles
simultaneously possessing plasmonic, antioxidant and antimicrobial properties
VL - 13
ID - 6638
ER -
TY - JOUR
AB - The current global occurrence of dengue infection annually is approximately
400 million, with a case fatality rate of 2.5%. However, there are no antiviral
agents. Carica papaya leaf extract is known for its medicinal value, due to the
presence of organic compounds that possess antimicrobial, anti-inflammatory, and
antioxidant activities. This study determined the anti-dengue effect of C. papaya
leaf extract silver synthesized nanoparticles. In this study, aqueous and non-
aqueous extractions were carried out, followed by the synthesis of silver
nanoparticles as well as characterization through Fourier transform infrared
spectroscopy (FTIR) and scanning electron microscopy. The in vitro anti-dengue
effect was evaluated using a focus reduction neutralization test on kidney Vero E2
cell lines. In silico studies involved molecular docking to determine the potential
interactions between the bioactive compounds in C. papaya leaf extract and the
viral NS5 protein. C. papaya leaf methanol extract silver synthesized nanoparticle
was the most promising with an IC50 of 9.20 mu g/mL. Molecular docking showed 5,7
dimethoxycoumarin as the best ligand, with binding energy of -7.75 kcal/mol,
indicating high affinity for the NS5 protein. These results highlight that C.
papaya leaf methanol extract silver synthesized nanoparticles could be used to
inhibit dengue virus type 2 viral replication. However, we recommend further
studies to determine their toxicity and the safety profiles.
AN - WOS:000689892200001
AU - Bere, A. W.
AU - Mulati, O.
AU - Kimotho, J.
AU - Ng'ong'a, F.
C7 - 718
DA - AUG
DO - 10.3390/ph14080718
IS - 8
PY - 2021
SN - 1424-8247
ST - Carica papaya Leaf Extract Silver Synthesized Nanoparticles Inhibit Dengue
Type 2 Viral Replication In Vitro
T2 - PHARMACEUTICALS
TI - Carica papaya Leaf Extract Silver Synthesized Nanoparticles Inhibit Dengue
Type 2 Viral Replication In Vitro
VL - 14
ID - 6120
ER -
TY - JOUR
AB - The current global occurrence of dengue infection annually is approximately
400 million, with a case fatality rate of 2.5%. However, there are no antiviral
agents. Carica papaya leaf extract is known for its medicinal value, due to the
presence of organic compounds that possess antimicrobial, anti-inflammatory, and
antioxidant activities. This study determined the anti-dengue effect of C. papaya
leaf extract silver synthesized nanoparticles. In this study, aqueous and non-
aqueous extractions were carried out, followed by the synthesis of silver
nanoparticles as well as characterization through Fourier transform infrared
spectroscopy (FTIR) and scanning electron microscopy. The in vitro anti-dengue
effect was evaluated using a focus reduction neutralization test on kidney Vero E2
cell lines. In silico studies involved molecular docking to determine the potential
interactions between the bioactive compounds in C. papaya leaf extract and the
viral NS5 protein. C. papaya leaf methanol extract silver synthesized nanoparticle
was the most promising with an IC50 of 9.20 µg/mL. Molecular docking showed 5,7
dimethoxycoumarin as the best ligand, with binding energy of −7.75 kcal/mol,
indicating high affinity for the NS5 protein. These results highlight that C.
papaya leaf methanol extract silver synthesized nanoparticles could be used to
inhibit dengue virus type 2 viral replication. However, we recommend further
studies to determine their toxicity and the safety profiles. © 2021 by the authors.
Licensee MDPI, Basel, Switzerland.
AU - Bere, A. W.
AU - Mulati, O.
AU - Kimotho, J.
AU - Ng’Ong’A, F.
C7 - 718
DB - Scopus
DO - 10.3390/ph14080718
IS - 8
KW - Antiviral activity
Carica papaya
Dengue virus
5,7 dimethoxycoumarin
Carica papaya extract
chlorogenic acid
coumarin derivative
kaempferol
methanol
nonstructural protein 5
protocatechuic acid
quercetin
silver nanoparticle
unclassified drug
animal cell
antiviral activity
Article
Chlorocebus
computer model
controlled study
Dengue virus 2
drug synthesis
epithelium cell
human
IC50
in vitro study
kidney cell
molecular docking
nonhuman
plant leaf
plaque reduction neutralization test
protein interaction
Vero cell line
virostatic activity
virus neutralization test
virus replication
M3 - Article
PY - 2021
ST - Carica papaya leaf extract silver synthesized nanoparticles inhibit dengue
type 2 viral replication in vitro
T2 - Pharmaceuticals
TI - Carica papaya leaf extract silver synthesized nanoparticles inhibit dengue
type 2 viral replication in vitro
85111965105&doi=10.3390%2fph14080718&partnerID=40&md5=23448e59fd46d5a19111eb497db5d
2db
VL - 14
ID - 5255
ER -
TY - JOUR
AB - So far, it was supposed that the increase of electrical impedance following
cochlear implant (CI) insertion was due to technical defects of the electrode,
inflammatory and/or formation of scar tissue along the electrode. However, it was
recently reported that corrosion of the platinum electrode contacts may be the
reason for high impedances. It could be shown that platinum particles were stripped
from the electrode surfaces. Its potential cytotoxic effects within the inner ear
remains to be examined. In this study in vitro cell culture models of the mouse
organ of Corti cell line (HEI-OC1) and the spiral ganglion (SG) cells derived from
the cochleae neonatal rats were used to investigate the effects of the
polyvinylpyrrolidone coated platinum nanoparticles (Pt-NPPVP, 3 nm) on cell
metabolism, neuronal survival and neurite outgrowth. Our data revealed no decrease
of the metabolic activity of the HEI-OC1 cells at Pt-NPPVP concentrations between
50-150 mu g/ml. Also, staining with Calcein AM/EthD demonstrated prevalent presence
of vital cells. As shown by transmission electron microscopy no Pt-NPPVP could be
found at the cell surface or in the cytosol of the HEI-OC1 cells. Similarly, the SG
cells exposed to 20-100 mu g/ml Pt-NPPVP did not show any reduced survival rate and
neurite outgrowth following staining of the neurofilament antigen even at the
highest Pt-NPPVP concentration. Although the SG cells were exposed to Pt-NPPVP for
further 72 h and 96 h immunocytochemical staining of the glial cells and
fibroblasts presented normal cell morphology and growth independently of the
cultivation period. Our data indicates that the used Pt-NPPVP do not trigger the
cellular uptake and, thus, presumable do not initiate apoptotic pathways in cells
of the organ of Corti cell line or the auditory nerve. The protection mechanisms to
the Pt-NPPVP interactions remain to be clarified.
AN - WOS:000984510200023
AU - Berger, E.
AU - Brandes, G.
AU - Reifenrath, J.
AU - Lenarz, T.
AU - Durisin, M.
AU - Wissel, K.
DA - APR 24
DO - 10.1371/journal.pone.0284794
IS - 4
PY - 2023
SN - 1932-6203
ST - In vitro impact of platinum nanoparticles on inner ear related cell culture
models
T2 - PLOS ONE
TI - In vitro impact of platinum nanoparticles on inner ear related cell culture
models
VL - 18
ID - 6672
ER -
TY - JOUR
AB - The present study focused on synthesizing, characterization, and evaluating
an in vitro anti-inflammatory and cytotoxic potential of green synthesized silver
nanoparticles (TB-Ag NPs) from aqueous stem bark extract of Terminalia brownii (TB-
AQ). The TB-Ag NPs were characterized by ultraviolet (UV)-visible, Fourier-
transform infrared spectroscopy (FTIR), dynamic light X-ray diffraction, and
energy-dispersive X-ray spectroscopy, as well as scanning and transmission electron
microscopy. The in vitro anti-inflammatory and cytotoxic potential of TB-Ag NPs and
TB-AQ were evaluated against RAW 264.7 macrophage and MDA-MB-231 triple-negative
breast cancer cells, respectively, by using 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide (MTT) assay. Further, the inhibitory effect on LPS-
induced production of inflammatory mediators PGE2 and NO in RAW 264.7 cell lines
was evaluated. The results showed that TB-Ag NPs were crystalline with face-
centered spherical polydispersed shaped nanoparticles with an average size between
20 and 67 ± 0.5 nm. Also, TB-Ag NPs had no cytotoxic effect on RAW cells (normal
healthy cells) in the range of 6.25–50 µg/mL. Besides, TB-Ag NPs at 50 µg/mL
concentration exhibited 67.02% of cytotoxic effect against MDA-MB-231 cells with
observed IC50 values of 29.08 µg/mL. TB-Ag NPs have shown significantly in
vitro anti-inflammatory activities by exhibiting dose-dependent NO and PGE2
inhibitory activities with IC50 values of 32.82 µg/mL and 67.25 µg/mL,
respectively. This study concluded that the novel green synthesized TB-Ag NPs can
be used as a potential novel anti-inflammatory and cytotoxicity agent to treat
inflammatory-related diseases and inflammatory breast cancer (invasive ductal
carcinoma) with biocompatible nature by targeting the tumor environment. © 2021,
The Author(s), under exclusive licence to Springer Science+Business Media, LLC,
part of Springer Nature.
AU - Berihu, H. T.
AU - Welderfael, T.
AU - Tekluu, B.
AU - Gopalakrishnan, V. K.
AU - Rao, M. R.
AU - Kumar, P. P. N. V.
AU - Shameem, U.
AU - Dogulas, P. J.
AU - Chaithanya, K. K.
DB - Scopus
DO - 10.1007/s12668-021-00885-8
IS - 4
MDA-MB-231breast cancer cell line
RAW macrophages
Terminalia brownii
Biocompatibility
Cell culture
Cells
Diseases
Energy dispersive spectroscopy
Metal nanoparticles
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
arginine methyl ester
carbon dioxide
cardiac glycoside
deionized water
dimethyl sulfoxide
distilled water
flavonoid
formazan
gallic acid
lipopolysaccharide
nitric oxide
penicillin derivative
phenol
phytochemical
plant extract
prostaglandin E2
silver nanoparticle
silver nitrate
sodium carbonate
streptomycin
sulfanilamide
tannin
toll like receptor
Energy dispersive X ray spectroscopy
Inflammatory breast cancers
Invasive ductal carcinomata
Raw 264.7 macrophages
Scanning and transmission electron microscopy
Triple-negative breast cancers
Article
bark
breast cancer
cell culture
cell density
cell viability
centrifugation
crystal
cytotoxicity
fetal bovine serum
incubation time
macrophage
MTT assay
nonhuman
RAW 264.7 cell line
Soxhlet extraction
triple negative breast cancer
tumor microenvironment
X ray diffraction
zeta potential
Silver metallography
M3 - Article
PY - 2021
SP - 998-1016
ST - Anti-inflammatory and Cytotoxicity activities of Green Synthesized Silver
Nanoparticles from Stem Bark of Terminalia brownii
T2 - BioNanoScience
TI - Anti-inflammatory and Cytotoxicity activities of Green Synthesized Silver
Nanoparticles from Stem Bark of Terminalia brownii
85113309220&doi=10.1007%2fs12668-021-00885-
8&partnerID=40&md5=7e28be98f8f6c29e49f726d49c549f59
VL - 11
ID - 5213
ER -
TY - JOUR
AB - Biofilms are matrices synthesized by bacteria containing polysaccharides,
DNA, and proteins. The development of biofilms in infectious processes can induce a
chronic inflammatory response that may progress to the destruction of tissues. The
treatment of biofilms is difficult because they serve as a bacterial mechanism of
defense and high doses of antibiotics are necessary to treat these infections with
limited positive results. It has been demonstrated that photothermal therapy using
gold nanorods (AuNRs) is an attractive treatment because of its anti-biofilm
activity. The purpose of this work was to generate a novel chitosan-based hydrogel
embedded with AuNRs to evaluate its anti-biofilm activity. AuNRs were synthesized
by the seed-mediated growth method and mixed with the chitosan-based hydrogel.
Hydrogels were characterized and tested against two bacterial strains by
irradiating the produced biofilm in the presence of the nanoformulation with a
laser adjusted at the near infrared spectrum. In addition, the safety of the
nanoformulation was assessed with normal human gingival fibroblasts. Results showed
that a significant bacterial killing was measured when biofilms were exposed to an
increase of 10 degrees C for a short time of 2 min. Moreover, no cytotoxicity was
measured when normal gingival fibroblasts were exposed to the nanoformulation using
the bactericidal conditions. The development of the reported formulation can be
used as a direct application to treat periodontal diseases or biofilm-produced
bacteria that colonize the oral cavity.
AN - WOS:000505634000004
AU - Bermudez-Jimenez, C.
AU - Nino-Martinez, N.
AU - Patino-Marin, N.
AU - Martinez-Gutierrez, F.
AU - Ruiz, F.
AU - Bach, H.
AU - Martinez-Castanon, G.
DA - FEB
DO - 10.1002/jbm.b.34392
IS - 2
PY - 2020
SN - 1552-4973
1552-4981
SP - 333-342
ST - Effective control of biofilms by photothermal therapy using a gold nanorod
hydrogel
T2 - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
TI - Effective control of biofilms by photothermal therapy using a gold nanorod
hydrogel
VL - 108
ID - 6775
ER -
TY - JOUR
AB - The biological effects of acute particulate air pollution exposure in host
innate immunity remain obscure and have relied largely on in vitro models. We
hypothesized that single acute exposure to ambient or engineered particulate matter
(PM) in the absence of other secondary stimuli would activate lung dendritic cells
(DC) in vivo and provide information on the early immunological events of PM
exposure and DC activation in a mouse model naïve to prior PM exposure. Activation
of purified lung DC was studied following oropharyngeal instillation of ambient
particulate matter (APM). We compared the effects of APM exposure with that of
diesel-enriched PM (DEP), carbon black particles (CBP) and silver nanoparticles
(AgP). We found that PM species induced variable cellular infiltration in the lungs
and only APM exposure induced eosinophilic infiltration. Both APM and DEP activated
pulmonary DC and promoted a Th2-type cytokine response from naïve CD4+ T cells ex
vivo. Cultures of primary peribronchial lymph node cells from mice exposed to APM
and DEP also displayed a Th2-type immune response ex vivo. We conclude that
exposure of the lower airway to various PM species induces differential
immunological responses and immunomodulation of DC subsets. Environmental APM and
DEP activated DC in vivo and provoked a Th2 response ex vivo. By contrast, CBP and
AgP induced altered lung tissue barrier integrity but failed to stimulate CD4+ T
cells as effectively. Our work suggests that respirable pollutants activate the
innate immune response with enhanced DC activation, pulmonary inflammation and Th2-
immune responsiveness. Copyright © 2010 S. Karger AG.
AU - Bezemer, G. F. G.
AU - Bauer, S. M.
AU - Oberdörster, G.
AU - Breysse, P. N.
AU - Pieters, R. H. H.
AU - Georas, S. N.
AU - Williams, M. A.
DB - Scopus
DO - 10.1159/000321725
IS - 2
KW - Allergic immunity
Dendritic cell
Immunotoxicology
Inflammation
Innate immunity
Lung
Nanoparticles
Particulate matter
Toxicology
Air Pollutants
Animals
Carbon
Dendritic Cells
Immunity, Innate
Mice
Mice, Inbred C57BL
Particulate Matter
Pneumonia
Silver
Th2 Cells
Vehicle Emissions
carbon
diesel fuel
gamma interferon
interleukin 10
interleukin 4
interleukin 5
interleukin 6
silver nanoparticle
ambient air
animal cell
animal experiment
article
CD4+ T lymphocyte
cell activation
cell infiltration
cell interaction
cell viability
cytokine production
cytotoxicity
dendritic cell
environmental exposure
eosinophil
ex vivo study
exhaust gas
immunomodulation
in vivo study
innate immunity
lung alveolus cell type 2
lymph node cell
mouse
nonhuman
particulate matter
peribronchial lymph node cell
priority journal
protein secretion
T lymphocyte subpopulation
M3 - Article
PY - 2011
SP - 150-166
ST - Activation of pulmonary dendritic cells and Th2-type inflammatory responses
on instillation of engineered, environmental diesel emission source or ambient air
pollutant particles in vivo
T2 - Journal of Innate Immunity
TI - Activation of pulmonary dendritic cells and Th2-type inflammatory responses
on instillation of engineered, environmental diesel emission source or ambient air
pollutant particles in vivo
79951993777&doi=10.1159%2f000321725&partnerID=40&md5=abd78ffde6bf101e45d7a91a828a1b
2d
VL - 3
ID - 5703
ER -
TY - JOUR
AB - The aim of this study was to evaluate radiographically and histologically the
pulpal and periapical response to self-adhesive (Rely X (TM) Unicem) and self-
etching and self-curing (Multilink (R)) resin-based luting materials in deep
cavities in dogs' teeth. Deep class V cavities (0.5-mm-thick dentin) were prepared
in 60 canine premolars and the following materials were applied on cavity floor:
Groups I/V-RelyX (TM) Unicem; Groups II/VI-Multilink (R); Groups III/VII-zinc
phosphate cement (control) and; Groups IV/VIII-gutta-percha (control). Cavities
were restored with silver amalgam. Animals were euthanized after 10 days (groups I-
IV) and 90 days (groups V-VIII). Tooth/bone blocks were radiographed and processed
for histopathological evaluation of pulp and periapical tissue response to the
materials. All materials presented similar histopathological features and
radiographic findings at both periods. The pulp tissue was intact. The apical and
periapical regions and periodontal ligament thickness were normal. No inflammatory
cells, resorption of mineralized tissue (dentin, cementum, and alveolar bone) or
bacteria were observed. The lamina dura was intact and no areas of periapical bone
rarefaction or internal/external root resorption were observed radiographically. It
can be concluded that Rely X (TM) Unicem and Multilink (R) caused no adverse tissue
reactions and may be indicated for cementation of indirect restorations in deep
dentin cavities without pulp exposure. (C) 2015 Wiley Periodicals, Inc.
AN - WOS:000368253300007
AU - Bezzon, O. L.
AU - Rivera, D. S. H.
AU - Silva, R. A. B.
AU - Oliveira, D. S. B.
AU - Silva-Herzog, D.
AU - Nelson, P.
AU - Lucisano, M. P.
AU - Silva, L. A. B.
DA - DEC
DO - 10.1002/jemt.22590
IS - 12
PY - 2015
SN - 1059-910X
1097-0029
SP - 1098-1103
ST - Resin Luting Materials: Tissue Response in Dog's Teeth
T2 - MICROSCOPY RESEARCH AND TECHNIQUE
TI - Resin Luting Materials: Tissue Response in Dog's Teeth
VL - 78
ID - 6428
ER -
TY - JOUR
AB - Euphorbia antiquorum was known for its various therapeutic values like anti-
inflammatory, anti-diabetic activity etc. Preliminary phytochemicals screening of
plant extract disclosed the existence of various impor-tant bioactive constituents
in hexane, ethanol and aqueous extract of the plant. Aqueous extract of Euphorbia
antiquorum was used for silver and copper nanoparticle synthesis. Synthesized
silver and copper nanoparti-cle showed the absorbance line at 422 and 367 nm
respectively. Fourier-transform infrared spectroscopy analysis of synthesized
nanoparticles reported the presence of phenol groups, amines and esters. Shape and
morphology of the synthesized nanoparticles were characterized through Field
Emission Scanning Electron Microscopy which showed the spherical shaped
nanoparticles. Energy dispersive X-ray spectroscopy images showed the composition
of analysed nanoparticles. The antioxidant activity of synthesized silver and
copper nanoparticles of Euphorbia antiquorum by hydrogen peroxide scavenging
activity method using ascorbic acid as the standard showed effective scavenging
activity and indicated the potentiality of the plant as a source of natural
antioxidant. Cytotoxicity study of silver nanoparticles showed 73.22% of inhibition
at the concentra-tion of 1000 mg/ml whereas copper nanoparticles at the same
concentration showed 68.85% of inhibition against breast cancer cell line.(c) 2022
Published by Elsevier B.V. on behalf of SAAB.
AN - WOS:000878638200002
AU - Bharathi, S. V.
AU - Das, M.
C6 - OCT 2022
DA - DEC
DO - 10.1016/j.sajb.2022.10.017
PY - 2022
SN - 0254-6299
1727-9321
SP - 410-416
ST - Cytotoxicity effect of nanoparticles of Euphorbia antiquorum on breast cancer
cell line
T2 - SOUTH AFRICAN JOURNAL OF BOTANY
TI - Cytotoxicity effect of nanoparticles of Euphorbia antiquorum on breast cancer
cell line
VL - 151
ID - 6153
ER -
TY - JOUR
AB - Objectives: The present research focuses on the in vitro anti-proliferative,
and in silico ribonucleotide reductase and pharmacokinetics studies of twelve
heteroleptic metal complexes of the general formulae [Ag(L1−4)(ibu)] (1–4) and
[M(L1−4)(ibu)2] (5–12), where L1−4 = 2-(1-(4-substitutedphenyl)ethylidene)-N-
methylhydrazinecarbothioamide, ibu = non-steroidal anti-inflammatory drug
(ibuprofen), and M = Cu(II) and Ni(II). Methods: Various spectroscopic techniques
were used to authenticate the structure of the synthesized complexes. UV-Vis and
cyclic voltammetry techniques were used to analyse the stability and the reducing
ability of the complexes. In vitro anti-proliferative studies by MTT assay,
apoptotic behaviour and cellular uptake studies were investigated followed by the
in silico interaction with ribonucleotide reductase (RNR) enzyme. Results: The
spectral studies predicted distorted tetrahedral geometry around silver(I) ion and
distorted octahedral geometry around nickel(II) and copper(II) ions. The reducing
ability of the copper(II) complexes was analysed using ascorbic acid by UV-Vis and
cyclic voltammetry techniques, which authenticate the reducing ability of the
complexes and the possible interactions within the cells. The in vitro anti-
proliferative activity of the synthesized complexes against three cancerous
(estrogen positive (MCF-7), estrogen negative (MDA-MB-231) and pancreatic (PANC-1))
and one normal (MCF-10a) cell lines by MTT assay showed enhanced activity for
copper(II) complexes 11 and 12 containing the hydrophobic substituents. The
apoptotic and cellular uptake studies showed that the complex 12 is readily taken
up by PANC-1 cell lines and induces ROS-mediated mitochondrial and caspase-
dependent apoptosis. The in silico studies indicated hydrogen bonding, hydrophobic
and π-pair (π–π, π–σ and π–cation) interactions between the complexes and the
ribonucleotide reductase (RNR) enzyme. The in silico pharmacokinetics studies of
the complexes predicted the drug-likeness characteristics of the complexes.
Conclusion: The synthesized complexes are found to be less toxic to normal cells
and inhibit the growth of cancerous cells by inducing mitochondrial-mediated and
caspase dependent apoptotic pathway in PANC-1 cells. © 2023 Elsevier GmbH
AU - Bharathi, S.
AU - Mahendiran, D.
AU - Ahmed, S.
AU - Rahiman, A. K.
C7 - 127211
DB - Scopus
DO - 10.1016/j.jtemb.2023.127211
KW - Anti-proliferative activity
Heteroleptic complexes
In silico studies
NSAIDs
Ribonuleoside phosphate reductase (RNRs)
Copper
Ibuprofen
Ligands
Nickel
Ribonucleotide Reductases
Silver
Thiosemicarbazones
4 methyl 3 thiosemicarbazone derivative
ascorbic acid
copper
ibuprofen
nickel
ribonucleotide reductase
silver
thiosemicarbazone derivative
unclassified drug
ligand
apoptosis
Article
cyclic voltammetry
cytotoxicity
DNA repair
DNA replication
drug design
drug distribution
drug stability
drug structure
drug synthesis
electrochemistry
geometry
human
human cell
hydrogen bond
hydrophobicity
in vitro study
lipophilicity
MCF-7 cell line
molecular weight
MTT assay
PANC-1 cell line
protein expression
stoichiometry
M3 - Article
PY - 2023
ST - In vitro anti-proliferative, and in silico ribonucleotide reductase and
pharmacokinetics studies of heteroleptic silver(I), nickel(II) and copper(II)
complexes of 4-methyl-3-thiosemicarbazones and ibuprofen
T2 - Journal of Trace Elements in Medicine and Biology
TI - In vitro anti-proliferative, and in silico ribonucleotide reductase and
pharmacokinetics studies of heteroleptic silver(I), nickel(II) and copper(II)
complexes of 4-methyl-3-thiosemicarbazones and ibuprofen
85160416489&doi=10.1016%2fj.jtemb.2023.127211&partnerID=40&md5=927701f97d4cf313af0d
f35f6bccf199
VL - 79
ID - 4976
ER -
TY - JOUR
AB - The increase in drug-resistant strains of Staphylococcus aureus, especially
methicillin-resistant S. aureus (MRSA), has led to an increased rate of infection-
related mortality. The emergence of drug resistance has rendered many antibiotics
ineffective. The poor penetration and retention of antibiotics in mammalian cells
lead to recurrent latent infections. Thus, there is an increasing need for
biodegradable, non-toxic anti-infectives that are effective in treating MRSA
infections. Phytochemicals such as berberine (BBR) and curcumin (CCR) have long
been explored for their antibacterial activities, but their efficacy is often
limited due to low bioavailability, water solubility, and poor cell penetration.
When used in combination these antimicrobials did not show any synergistic effect
against MRSA. Here, both of them were co-encapsulated in liposomes (BCL) and
evaluated for biocompatibility, synergistic antimicrobial activity, intracellular
infections, associated inflammation, and on biofilms formed by MRSA. Co-
encapsulation of BBR and CCR in liposomes decreased their MICs by 87% and 96%,
respectively, as compared to their free forms with a FICI of 0.13, indicating
synergy between them. BCL inhibited the growth of MRSA and prevented biofilm
formation better than free drugs. Co-culture studies showed that intracellular
infection was reduced to 77% post BCL treatment. It also reduced the production of
pro-inflammatory cytokines by macrophages following infection. The liposomes were
found to be five times more efficient than clindamycin and can be used as a
potential antimicrobial carrier against intracellular infections.
AN - WOS:000612856700027
AU - Bhatia, E.
AU - Sharma, S.
AU - Jadhav, K.
AU - Banerjee, R.
DA - JAN 21
DO - 10.1039/d0tb02036b
IS - 3
PY - 2021
SN - 2050-750X
2050-7518
SP - 864-875
ST - Combinatorial liposomes of berberine and curcumin inhibit biofilm formation
and intracellular methicillin resistant Staphylococcus aureus infections and
associated inflammation
T2 - JOURNAL OF MATERIALS CHEMISTRY B
TI - Combinatorial liposomes of berberine and curcumin inhibit biofilm formation
and intracellular methicillin resistant Staphylococcus aureus infections and
associated inflammation
VL - 9
ID - 6762
ER -
TY - JOUR
AB - Engineered nanomaterials (ENMs) are being produced for an increasing number
of applications. Therefore, it is important to assess and categorize ENMs on the
basis of their hazard potential. The immune system is the foremost defence against
foreign bodies. Here we performed cytokine profiling of a panel of nineteen
representative ENMs procured from the Joint Research Centre (JRC) and commercial
sources. Physicochemical characterization was performed using dynamic light
scattering. The ENMs were all shown to be endotoxin content free. The human
macrophage-differentiated THP.1 cell line was employed for cytotoxicity screening
and based on the calculated IC50 values, the multi-walled carbon nanotubes
(MWCNTs), ZnO, Ag and SiO2 NMs were found to be the most cytotoxic while single-
walled carbon nanotubes (SWCNTs), TiO2, BaSO4 and CeO2 NMs, as well as the
nanocellulose materials, were non-cytotoxic (at doses up to 100 µg/mL). Multiplex
profiling of cytokine and chemokine secretion indicated that the TiO2, SiO2, BaSO4,
CeO2 and nanocellulose materials induced potent inflammatory responses at sub-
cytotoxic doses. Hierarchical clustering of cytokine responses coupled with pathway
analysis demonstrated that the panel of ENMs could be segregated into two distinct
groups characterized by activation and deactivation, respectively, of PPAR
(peroxisome proliferator-activated receptor)/LXR (liver X receptor/retinoid X
receptor) nuclear receptor pathways (NRPs). Furthermore, using rosiglitazone, a
selective PPAR-γ agonist, we could show that PPAR-γ played an important role in the
activation of inflammatory responses in cells exposed to TiO2 and SiO2 NMs. These
studies show that ENMs of diverse chemical compositions can be grouped according to
their inflammatory potential. © 2017 The Author(s). Published by Informa UK
Limited, trading as Taylor & Francis Group.
AU - Bhattacharya, K.
AU - Kiliç, G.
AU - Costa, P. M.
AU - Fadeel, B.
DB - Scopus
DO - 10.1080/17435390.2017.1363309
IS - 6
KW - cytotoxicity
hierarchical clustering
in vitro screening
inflammation
Nanomaterials
Cell Line
Cell Survival
Cluster Analysis
Cytokines
Hazardous Substances
Humans
Macrophages
Nanostructures
Nanotubes, Carbon
Particle Size
cell nucleus receptor
cerium oxide nanoparticle
chemokine
cytokine
endotoxin
liver X receptor
multi walled nanotube
nanomaterial
peroxisome proliferator activated receptor
retinoid X receptor
rosiglitazone
silica nanoparticle
silver nanoparticle
single walled nanotube
carbon nanotube
Article
cytokine release
cytokine response
cytotoxicity test
human
human cell
IC50
in vitro study
macrophage
particle size
physical chemistry
priority journal
risk assessment
zeta potential
cell line
cell survival
chemistry
classification
cluster analysis
dangerous goods
dose response
drug effects
immunology
toxicity
M3 - Article
PY - 2017
SP - 809-826
ST - Cytotoxicity screening and cytokine profiling of nineteen nanomaterials
enables hazard ranking and grouping based on inflammogenic potential
T2 - Nanotoxicology
TI - Cytotoxicity screening and cytokine profiling of nineteen nanomaterials
enables hazard ranking and grouping based on inflammogenic potential
85027882619&doi=10.1080%2f17435390.2017.1363309&partnerID=40&md5=18a8487e3d6de498f0
35e46bcd6bd51c
VL - 11
ID - 5540
ER -
TY - JOUR
AB - Background: Nanocrystalline silver has both antimicrobial and anti-
inflammatory properties. However, the exact mechanisms underlying these activities
are not known. Objectives: The objectives of this study were to assess the anti-
inflammatory effects of nanocrystalline silver using a murine model of allergic
contact dermatitis, compare the effects with those of tacrolimus and a high potency
steroid, and to relate the effects to modulation of pro-inflammatory cytokines and
apoptosis of inflammatory cells. Methods: Dermatitis was induced on the ears of
BALB/c mice using dinitrofluorobenzene. Topical treatment, including vehicles, 1%
nanocrystalline silver cream, tacrolimus ointment and a high potency steroid, was
applied once a day for 4 days. Ear swelling was measured and the erythema was
evaluated daily. After 4 days of treatment the mice were killed and samples from
the ears were collected for histological and immunohistochemical examination,
terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labelling
(TUNEL) staining and extraction of total RNA for reverse transcriptase polymerase
chain reaction (RT-PCR). Results: Significant reductions of ear swelling, erythema
and histopathological inflammation in mice ears were observed after 4 days of
treatment with 1% nanocrystalline silver cream, tacrolimus ointment or a high
potency steroid with no significant difference among them. Both RT-PCR and
immunohistochemical staining of sections from ear biopsies demonstrated that
nanocrystalline silver, tacrolimus and steroid significantly suppressed the
expression of tumour necrosis factor (TNF)-α and interleukin (IL)-12. TUNEL
staining demonstrated a significant increase in the numbers of apoptotic cells in
material from the group treated with nanocrystalline silver when compared with that
from groups treated with vehicle, tacrolimus or steroid. Conclusions: This study
demonstrates that nanocrystalline silver inhibits allergic contact dermatitis in
mice, similar to steroid and tacrolimus. Nanocrystalline silver suppresses the
expression of TNF-α and IL-12 and induces apoptosis of inflammatory cells;
mechanisms by which nanocrystalline silver may exert its anti-inflammatory effects.
© 2005 British Association of Dermatologists.
AU - Bhol, K. C.
AU - Schechter, P. J.
DB - Scopus
DO - 10.1111/j.1365-2133.2005.06575.x
IS - 6
KW - Allergic contact dermatitis
Anti-inflammatory
Apoptosis induction
Inflammatory cytokines suppression
Nanocrystalline silver
Animals
Apoptosis
Crystallization
Dermatitis, Allergic Contact
Dinitrofluorobenzene
Female
Glucocorticoids
Immunosuppressive Agents
In Situ Nick-End Labeling
Interleukin-12
Mice
Mice, Inbred BALB C
Models, Animal
Nanostructures
Ointments
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Silver
Staining and Labeling
Tacrolimus
1 fluoro 2,4 dinitrobenzene
gamma interferon
interleukin 1
interleukin 12
messenger RNA
nanocrystalline silver
nanoparticle
protein p35
protein p40
RNA
silver derivative
steroid
tacrolimus
ulobetasol propionate
unclassified drug
animal experiment
animal model
animal tissue
apoptosis
article
cell assay
controlled study
cream
drug effect
edema
erythema
experimental model
female
histopathology
inflammation
inflammatory cell
mouse
mouse strain
nick end labeling
nonhuman
ointment
priority journal
protein expression
RNA extraction
scoring system
skin allergy
M3 - Article
PY - 2005
SP - 1235-1242
ST - Topical nanocrystalline silver cream suppresses inflammatory cytokines and
induces apoptosis of inflammatory cells in a murine model of allergic contact
dermatitis
T2 - British Journal of Dermatology
TI - Topical nanocrystalline silver cream suppresses inflammatory cytokines and
induces apoptosis of inflammatory cells in a murine model of allergic contact
dermatitis
21644473217&doi=10.1111%2fj.1365-
2133.2005.06575.x&partnerID=40&md5=40f55266bd0b57f247dbcbd8dbe5eca4
VL - 152
ID - 5801
ER -
TY - JOUR
AB - In this article, we report a simple, cost-effective and eco-friendly method
of airbrushing for the fabrication of antibacterial composite nanofibers using
Nylon-6 and silver chloride (AgCl). The Nylon-6 is a widely used polymer for
various biomedical applications because of its excellent biocompatibility and
mechanical properties. Similarly, silver has also been known for their
antibacterial, antifungal, antiviral, and anti-inflammatory properties. In order to
enhance the antibacterial functionality of the Nylon-6, composite nanofibers in
combination with AgCl have been fabricated using airbrush method. The chemical
functional groups and morphological studies of the airbrushed Nylon-6/AgCl
composite nanofibers were carried out by FTIR and SEM, respectively. The
antibacterial activity of airbrushed Nylon-6/AgCl composite nanofibers was
evaluated using Gram + ve (Staphylococcus aureus) and Gram -ve (Escherichia coli)
bacterial strains. The results showed that the airbrushed Nylon-6/AgCl composite
nanofibers have better antibacterial activity against the tested bacterial strains
than the airbrushed Nylon-6 nanofibers. Therefore, the airbrushed Nylon-6/AgCl
composite nanofibers could be used as a potential antibacterial scaffolding system
for tissue engineering and regenerative medicine.
AN - WOS:000426050500088
AU - Bhullar, S. K.
AU - Rana, D.
AU - Ozsel, B. K.
AU - Orhan, M.
AU - Jun, M. B. G.
AU - Buttar, H. S.
AU - Ostrovidov, S.
AU - Ramalingam, M.
DA - APR
DO - 10.1166/jnn.2018.14376
IS - 4
PY - 2018
SN - 1533-4880
1533-4899
SP - 2951-2955
ST - Development of Silver-Based Bactericidal Composite Nanofibers by Airbrushing
T2 - JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
TI - Development of Silver-Based Bactericidal Composite Nanofibers by Airbrushing
VL - 18
ID - 6247
ER -
TY - JOUR
AB - The recent need for remote health wellness monitoring has led to the
extensive use of wearable sensors. Owing to their increased use, these sensors are
required to exhibit both functionality and safety to the user. A major component in
the fabrication of these sensors and their associated circuitry is the use of
metallic/organic conductive inks. However, very less is known about the interfacial
and molecular interactions of these inks with biological matter as they can result
in an inflammatory reaction to the user. Significant efforts are thus needed to
explore and improve the bio-acceptability of such conductive ink-based wearable
sensors. The present study investigates the biocompatibility of encapsulated and
non-encapsulated wearable electrochemical sensors used for sensing uric acid as a
biomarker for wound healing fabricated using screen-printing technique. Ionic
release of metallic ions was investigated first to understand the susceptibility of
the conductive inks towards ionic leaching when in contact with a fluid. Time-lapse
investigation using ICPS (inductive couple plasma spectroscopy) shows a high
concentration (607.31 ppb) of leached silver (Ag+) ions from the non-encapsulated
sensors. The cell viability data suggests a 2.5-fold improvement in the sensor
biocompatibility for an encapsulated sensor. While the carbon ink shows negligible
effect on cell viability, the silver ink elicits significant decrease (< 50%) in
cell viability at concentrations higher than 2 mg ml-1. The toxicity pathway of
these sensors was further determined to be through the generation of reactive
oxygen species resulting in over 20% apoptotic cell death. Our results show that
the lower biocompatibility of the non-encapsulated sensor attributes to the higher
leaching of Ag+ ions from the printed inks which elicits several different
inflammatory pathways. This work highlights the importance biocompatibility
evaluation of the material used in sensor fabrication to develop safe and
sustainable sensors for long-term applications. © 2022, This is a U.S. Government
work and not under copyright protection in the US; foreign copyright protection may
apply.
AU - Bhushan, P.
AU - Kamat, V.
AU - Abrol, I.
AU - Kaushik, A.
AU - Bhansali, S.
C7 - 10782
DB - Scopus
DO - 10.1038/s41598-022-13810-0
IS - 1
KW - Humans
Inflammation
Ink
Ions
Silver
Wearable Electronic Devices
ink
ion
silver
electronic device
human
inflammation
M3 - Article
PY - 2022
ST - Bio-acceptability of wearable sensors: a mechanistic study towards evaluating
ionic leaching induced cellular inflammation
T2 - Scientific Reports
TI - Bio-acceptability of wearable sensors: a mechanistic study towards evaluating
ionic leaching induced cellular inflammation
85132938484&doi=10.1038%2fs41598-022-13810-
0&partnerID=40&md5=8f745c6cda74cc9ade4d599b3ed83174
VL - 12
ID - 4971
ER -
TY - JOUR
AB - 24 hours after administration of Intration in toxic doses, necrosis takes
place with calcifications of single fibres, focal acidophilic degeneration, oedema
of stroma and glycogen loss in the muscles of ventricles and auricles of the heart.
The necrosis foci were sometimes accompanied by an inflammatory reaction. In the
heart valves the oedema of stroma, subendothelial inflammatory infiltrations, as
well as degeneration and desquamation of endothelium cells were found. After 8 days
a significant decrease of the extent of foci of acidophilic degeneration occurred.
In the existing foci, however, coagulation necrosis of the cardiac muscle fibres
was evident. In the valves, besides oedema, the intensification of the inflammatory
reaction and focal fibrousness, as well as endothelium regeneration were found.
After PAM administration, necrosis and calcification of fibres were not found;
there were, however, extensive foci of acidophilic degeneration of cardiac muscle
fibers and more numerous inflammatory infiltrations in the valves. After 8 days,
the cardiac muscle fibres in the state of coagulation necrosis were more numerous
than in the untreated animals. Oedema and the increase of capillary vessel
endothelia, as well as ischaemia of cardiac muscle were also observed. The authors
discuss the possibility of the complex action of Intration, through the stimulation
of the parasympathetic system, as well as directly upon the cell. PAM abolishes
probably only the pharmacological action of Intration, but provokes intensification
of secondary changes, which could follow the action of the products of
decomposition of Intration and of PAM alike.
AU - Biernat, S.
AU - Giermaziak, H.
DB - Scopus
IS - 4
KW - intration
methenamine periodate silver
pam
thiometon
unclassified drug
dose response
drug toxicity
heart
histology
in vitro study
intoxication
intravenous drug administration
rat
theoretical study
M3 - Article
PY - 1976
SP - 241-251
ST - Pathomorphological changes in rat heart after administration of intration in
toxic doses and after large doses of PAM (Polish)
T2 - Medycyna Pracy
TI - Pathomorphological changes in rat heart after administration of intration in
toxic doses and after large doses of PAM (Polish)
0017139964&partnerID=40&md5=38f40b7a526341e0809f1b1562db0a85
VL - 27
ID - 5800
ER -
TY - JOUR
AB - SUMMARY: Liver transplantation is the only available method to treat liver
failure induced by chronic liver injury. We sought to determine whether the
angiotensin-converting enzyme inhibitor, captopril, can inhibit the development of
chronic liver injury induced by the hepatotoxic agent thioacetamide (TAA) in
association with the suppression of inflammation (hsCRP, TNF-α, and IL-6) /
hypoxia- inducible factor 1-alpha (HIF-1α) / profibrosis (TIMP-1, MMP-9, and α-SMA)
axis that mediates liver injury. Therefore, the model group of rats was injected
for eight weeks with 200 mg/kg TAA starting at week two. The protective group was
pretreated with 150 mg/ kg captopril daily for two weeks prior to TAA injections
and continued receiving both capropril and TAA agents until being humanely
scrificed at week 10. We observed a substantial damage to liver tissue in the model
group as demonstrated by a significant (p<0.0001) increase in blood and hepatic
tissue levels of high sensitivity C-reactive protein (hsCRP), tumor necrosis
factor-a (TNF-α), interleukin- 6 (L-6), HIF-1α, tissue inhibitor of
metalloproteinases-1 (TIMP-1), matrix metalloproteinase-9 (MMP-9), alpha-smooth
muscle actin (α-SMA), alanine aminotransferase (ALT), and aspartate
aminotransferase (AST). All these parameters were significantly (p<0.0244)
protected by captopril. Also, a significant (p<0.0001) positive correlation was
observed between a-SMA (profibrosis) and the serum and tissue levels of hsCRP, TNF-
α, HIF-1α, TIMP-1, MMP-9, and ALT. Thus, these findings suggest that the induction
of chronic liver injury by the hepatotoxic compound, TAA is associated with the
upregulation of inflammation/HIF-1α/profibrosis, with captopril exhibiting
beneficial hepatic pleotropic effects.
El trasplante de hígado es el único método disponible para tratar la insuficiencia
hepática inducida por una lesión hepática crónica. Buscamos determinar si el
inhibidor de la enzima convertidora de angiotensina, captopril, puede inhibir el
desarrollo de lesión hepática crónica inducida por el agente hepatotóxico
tioacetamida (TAA) en asociación con la supresión de la inflamación (hsCRP, TNF-α e
IL-6) / factor inducible por hipoxia 1-alfa (HIF-1α) / profibrosis (TIMP-1, MMP-9 y
α- SMA) eje que media la lesión hepática. Por lo tanto, al grupo modelo de ratas se
le inyectó durante ocho semanas 200 mg/kg de TAA a partir de la semana dos. El
grupo protector fue pretratado con 150 mg/kg de captopril al día durante dos
semanas antes de las inyecciones de TAA y continuó recibiendo capropril y agentes
TAA hasta que fue sacrificado en la semana 10. Observamos un daño sustancial en el
tejido hepático en el grupo modelo, como lo demuestra un aumento significativo
(p<0,0001) de los niveles en sangre y tejido hepático de proteína C reactiva de
alta sensibilidad (hsCRP), factor de necrosis tumoral-α (TNF-a), interleucina-6 (L-
6), HIF-1α, inhibidor tisular de metaloproteinasas-1 (TIMP-1), metaloproteinasa de
matriz-9 (MMP-9), actina de músculo liso alfa (α-SMA), alanina aminotransferasa
(ALT) y aspartato aminotransferasa (AST). Todos estos parámetros estaban
significativamente (p<0,0244) protegidos por captopril. Además, se observó una
correlación positiva significativa (p<0,0001) entre α-SMA (profibrosis) y los
niveles séricos y tisulares de hsCRP, TNF-α, HIF-1α, TIMP- 1, MMP-9 y ALT. Por lo
tanto, estos hallazgos sugieren que la inducción de daño hepático crónico por el
compuesto hepatotóxico, TAA, está asociada con la regulación al alza de la
inflamación/HIF-1α/profibrosis, con captopril exhibiendo efectos pleotrópicos
hepáticos beneficiosos.
AD - Bin-Jaliah, Ismaeel
King Khalid University. College of Medicine. Department of Physiology. Abha. SA
AU - Bin-Jaliah, Ismaeel
C1 - 20230707
DA - 2023/04
DB - LILACS
DO - 10.4067/S0717-95022023000200362
IS - 2
KW - HIF-1alpha
Inflamación
Inflammation
Lesión hepática
Liver injury
Model
Modelo
Profibrosis
Rat
Rata
Thioacetamide
Tioacetamida
LA - en
PY - 2023
SN - 0717-9367
SP - 362-367
ST - Captopril inhibits thioacetamide-induced chronic liver injury associated with
the suppression of inflammation / hypoxia- inducible factor 1-alpha / profibrogenic
axis-mediated hepatotoxicity in rats
TI - Captopril inhibits thioacetamide-induced chronic liver injury associated with
the suppression of inflammation / hypoxia- inducible factor 1-alpha / profibrogenic
axis-mediated hepatotoxicity in rats
TT - Captopril inhibe la lesión hepática crónica inducida por tioacetamida
asociada con la supresión de la inflamación/factor 1-alfa inducible por
hipoxia/hepatotoxicidad mediada por el eje profibrogénico en rata
VL - 41
ID - 4914
ER -
TY - JOUR
AB - A proof of concept for designing multi-drug-delivery systems suitable for
self-drug-delivery is disclosed. Simple coordination chemistry was employed to
anchor two kinds of drugs namely isoniazid (IZ - anti-tuberculosis), various non-
steroidal-anti-inflammatory-drugs (NSAIDs) namely ibuprofen-IBU, fenoprofen-FEN,
naproxen-NAP, diclofenac-DIC and mefenamic acid-MEF and Zn(NO3)(2) to synthesize a
series of 1D coordination polymers namely IZIBU, IZFEN, IZNAP, IZDIC and IZMEF
which were structurally characterized by single crystal X-ray diffraction (SXRD).
The coordination polymers wherein both types of drugs were anchored to Zn(II) metal
centers could easily be ground to nano-sized particles suitable for biological
studies by hand grinding in a mortar and pestle. Zone inhibition studies revealed
that all the coordination polymers possessed antibacterial properties against Gram
positive, Gram negative and mycobacteria namely Mycobacterium tuberculosis (M.tb).
Detailed studies carried out on IZDIC employing flow cytometry and confocal
microscopy under various staining conditions established that such antibacterial
activity was due to the generation of reactive oxygen species (ROS) such as nitric
oxide (NO) and also inhibition of mycolic acid leading to incomplete cell wall
formation. It was also established that IZDIC could indeed inhibit the growth of
M.tb within a mouse macrophage host cell namely RAW 264.7 thereby simulating the
treatment of Tuberculosis (TB) under in vitro conditions. Scratch assay and cell
cycle analysis on a human lung cancer cell line (A549) revealed its anti-cancer
property, thereby indicating its potential as a multidrug-delivery system. In vivo
toxicity assessment (serum parameters, histopathology, and haemolysis) carried out
on BALB/c mice showed that IZDIC was safe up to a concentration of 100 mg kg(-1).
Finally, a reasonably high yield in bulk synthesis, stability under high
temperature and humid conditions, tabletability and, slow and sustained release of
the drug component of IZDIC suggested its suitability in real-life applications as
multi-drug-delivery systems.
AN - WOS:000853010200001
AU - Biswas, P.
AU - Datta, H. K.
AU - Dastidar, P.
C6 - AUG 2022
DA - OCT 25
DO - 10.1039/d2bm00752e
IS - 21
PY - 2022
SN - 2047-4830
2047-4849
SP - 6201-6216
ST - Designing coordination polymers as multi-drug-self-delivery systems for
tuberculosis and cancer therapy: in vitro viability and in vivo toxicity assessment
T2 - BIOMATERIALS SCIENCE
TI - Designing coordination polymers as multi-drug-self-delivery systems for
tuberculosis and cancer therapy: in vitro viability and in vivo toxicity assessment
VL - 10
ID - 6558
ER -
TY - JOUR
AB - Pellets of a glass ionomer-silver cement and a zinc oxide-eugenol cement were
implanted into the soft tissues and bones of 30 rats. Following experimental
periods of 14, 30, and 80 days, the animals were killed and tissue sections were
prepared. The responses to each of the materials initially and at 30 days consisted
of mild inflammation. No severe inflammatory responses were noted in any of the
groups. By 80 days, although mild inflammation persisted, the materials appeared to
be well tolerated. Bone apposition occurred in the glass ionomer-silver cement
group; the zinc oxide-eugenol group produced fibrosis. © 1989 The American
Association of Endodontists.
AU - Blackman, R.
AU - Gross, M.
AU - Seltzer, S.
DB - Scopus
DO - 10.1016/S0099-2399(89)80112-8
IS - 2
KW - Animal
Bone and Bones
Cermet Cements
Dental Alloys
Dental Cements
Glass Ionomer Cements
Rats
Rats, Inbred Strains
Silver
Support, Non-U.S. Gov't
glass ionomer
biomaterial
cement
dental alloy
silver
tooth cement
animal cell
animal model
biocompatibility
connective tissue
inflammation
mammal
nonhuman
rat
seal
tooth
ultrastructure
animal
article
bone
drug effect
rat strain
M3 - Article
PY - 1989
SP - 76-79
ST - An evaluation of the biocompatibility of a glass lonomer-silver cement in rat
connective tissue
TI - An evaluation of the biocompatibility of a glass lonomer-silver cement in rat
connective tissue
0024472057&doi=10.1016%2fS0099-2399%2889%2980112-
8&partnerID=40&md5=4cc12cd366b77dec2bac8532d5073db5
VL - 15
ID - 5798
ER -
TY - JOUR
AB - Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in the
treatment of rheumatoid arthritis and osteoarthritis, and are also indicated for
periarticular and musculoskeletal diseases. However, the use of NSAIDs is limited
by their toxicity. NSAIDs have a variable effect on the regeneration of cells and
extracellular matrix that depends on the dose used. In this work, we examined the
effect of naproxen, a NSAID, on tail fin regeneration in carp (Cyprinus carpio), a
teleost fish that is a good model for studying the growth of connective tissue in
vivo. We used histochemical, ultrastructural and morphometric analyses to assess
the synthesis, deposition and organization of the lepidotrichial extracellular
matrix components and the total area of regenerating fins, including lepidotrichia,
epidermis and connective tissue. Naproxen (15.6 mg/L in the tank water) did not
affect the formation of the epidermal cap and blastema, the differentiation of
blastemal cells in scleroblasts or the synthesis, deposition, organization and
mineralization of lepidotrichial matrix components. In addition, there was no
significant difference in the area of regenerated tissue between control and
naproxen-treated fishes. These results indicate that at the concentration tested,
naproxen had no effect on tail fin regeneration.
AD - Bõckelmann, Petra Karla
State University of Campinas. Institute of Biology. Department of Histology and
Embriology. Campinas. BR
Bechara, Ivanira José
State University of Campinas. Institute of Biology. Department of Histology and
Embriology. Campinas. BR
AU - Bõckelmann, Petra Karla
AU - Bechara, Ivanira José
C1 - 20070711
DA - 2007/03
DB - LILACS
IS - 1
LA - en
PY - 2007
SN - 0102-9010
SP - 17-24
ST - Histochemical and ultrastructural analysis of the action of naproxen on tail
fin regeneration in carp (Cyprinus carpio)
T2 - Braz. j. morphol. sci
TI - Histochemical and ultrastructural analysis of the action of naproxen on tail
fin regeneration in carp (Cyprinus carpio)
UR - https://pesquisa.bvsalud.org/portal/resource/pt/lil-497613
VL - 24
ID - 4954
ER -
TY - JOUR
AB - Here, we review several articles of original research published in 2008 that
concerned the toxicity of metal and carbon-based nanoparticles. Articles were
selected from the MEDLINE PubMed database, all published or pre-published during
2008 and relating to nanomaterials, -particles or -structures and toxicity or
health. From the 746 articles, we concentrated on research into carbonaceous
(carbon nanotubes [CNTs] and fullerenes) and metallic materials (pure metal,
oxides), because these nanomaterials are produced and used worldwide and are the
most relevant for public health. Unfortunately, due to the large variability in
materials used and methods used conflicting data are generated hampering the risk
assessment. © 2010 Informa UK Ltd.
AU - Boczkowski, J.
AU - Hoet, P.
DB - Scopus
DO - 10.3109/17435390903428844
IS - 1
KW - Human health
Nanomaterials
Nanoparticles
Toxicity
Animals
Cell Line
Humans
Nanostructures
Nanotubes, Carbon
Public Health
PubMed
Review Literature as Topic
Risk Assessment
Toxicology
United States
carbon
carbon nanotube
catalase
CXCL2 chemokine
endothelial leukocyte adhesion molecule 1
fullerene derivative
gelatinase A
gelatinase B
glutathione
heme oxygenase 1
interleukin 1
interleukin 1beta
interleukin 6
interleukin 8
macrophage inflammatory protein 2
messenger RNA
metal nanoparticle
metal oxide
nanomaterial
silicon dioxide
silver
thioredoxin reductase
titanium dioxide
unindexed drug
vascular cell adhesion molecule 1
cytotoxicity
genotoxicity
health hazard
human
inflammation
nanotoxicology
nonhuman
oxidative stress
priority journal
public health
review
risk assessment
toxicity testing
M3 - Review
PY - 2010
SP - 1-14
ST - What's new in nanotoxicology? Implications for public health from a brief
review of the 2008 literature
T2 - Nanotoxicology
TI - What's new in nanotoxicology? Implications for public health from a brief
review of the 2008 literature
77749316839&doi=10.3109%2f17435390903428844&partnerID=40&md5=dfb002adcdaabbebcd50b0
708610a1e3
VL - 4
ID - 5698
ER -
TY - JOUR
AB - Thrombin activates protease-activated receptor (PAR)-1 and induces a
myofibroblast phenotype in normal lung fibroblasts that resembles the phenotype of
scleroderma lung fibroblasts. We now demonstrate that PAR-1 expression is
dramatically increased in lung tissue from scleroderma patients, where it is
associated with inflammatory and fibroproliferative foci. We also observe that
thrombin induces resistance to apoptosis in normal lung fibroblasts, and this
process is regulated by protein kinase C (PKC)-epsilon but not by PKC-alpha.
Overexpression of a constitutively active (c-a) form of PAR-1 or PKC-epsilon
significantly inhibits Fas ligand-induced apoptosis in lung fibroblasts, whereas
scleroderma lung fibroblasts are resistant to apoptosis de novo. Thrombin
translocates p21Cip1/WAF1, a signaling molecule downstream of PKC, from the nucleus
to cytoplasm in normal lung fibroblasts mimicking the localization of p21Cip1/WAF1
in scleroderma lung fibroblasts. Overexpression of c-a PKC-alpha or PKC-epsilon
results in accumulation of p21Cip1/WAF1 in the cytoplasm. Depletion of PKC-alpha or
inhibition of mitogen-activated protein kinase (MAPK) blocks thrombin-induced DNA
synthesis in lung fibroblasts. Inhibition of PKC by calphostin or PKC-alpha, but
not PKC-, by antisense oligonucleotides prevents thrombin-induced MAPK
phosphorylation and accumulation of G(1) phase regulatory protein cyclin D1,
suggesting that PKC-alpha, MAPK, and cyclin D1 mediate lung fibroblast
proliferation. These data demonstrate that two distinct PKC isoforms mediate
thrombin-induced resistance to apoptosis and proliferation and suggest that
p21Cip1/WAF1 promotes both phenomena.
AN - WOS:000225618600022
AU - Bogatkevich, G. S.
AU - Gustilo, E.
AU - Oates, J. C.
AU - Feghali-Bostwick, C.
AU - Harley, R. A.
AU - Silver, R. M.
AU - Ludwicka-Bradley, A.
DA - JAN
DO - 10.1152/ajplung.00448.2003
IS - 1
PY - 2005
SN - 1040-0605
SP - L190-L201
ST - Distinct PKC isoforms mediate cell survival and DNA synthesis in thrombin-
induced myofibroblasts
T2 - AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
TI - Distinct PKC isoforms mediate cell survival and DNA synthesis in thrombin-
induced myofibroblasts
VL - 288
ID - 6535
ER -
TY - JOUR
AB - In this work, the natural latex extracted from Harconia speciosa was
incorporated with silver nanoparticles (AgNP) to compose a functional biomaterial
associating the intrinsic angiogenic activity of the latex and the antimicrobial
activity of AgNP. Tissue reaction after subcutaneous implantation in dorsum of rats
of membranes without AgNP and with 0.05%, 0.4% AgNP was compared at 3, 7 and 25
days. No statistically significant difference in the tissue response of the
different biomaterials was observed, indicating that AgNP did not interfere with
the inflammatory reaction (p > 0.05) or with the angiogenic activity of latex.
Biomembranes were also tested against bacterial biofilm formation by Staphylococcus
aureus and the antimicrobial activity of the new biomaterial can be found with
bacteria crenation (0.05% AgNP) and no biofilm deposition (0.4% AgNP). Therefore,
this biomaterial has interesting properties for the tissue repair process and may
be feasible for future applications as dressing. © 2020, Academia Brasileira de
Ciencias. All rights reserved.
AU - Bonete, J. M.
AU - Silva, G. D.
AU - Guidelli, É J.
AU - Gonçalves, P. J.
AU - Almeida, L. M.
AU - Baffa, O.
AU - Kinoshita, A.
C7 - e20191584
DB - Scopus
DO - 10.1590/0001-3765202020191584
IS - 4
KW - Antimicrobial activity
Biocompatibility
Biomembrane
Inflammation
Silver nanoparticle
Wound dressings
Animals
Biofilms
Latex
Metal Nanoparticles
Rats
Silver
antiinfective agent
biomaterial
latex
metal nanoparticle
silver
animal
biofilm
rat
M3 - Article
PY - 2020
SP - 1-14
ST - Tissue reaction and anti-biofilm action of new biomaterial composed of latex
from hancornia speciosa gomes and silver nanoparticles
T2 - Anais da Academia Brasileira de Ciencias
TI - Tissue reaction and anti-biofilm action of new biomaterial composed of latex
from hancornia speciosa gomes and silver nanoparticles
85096375010&doi=10.1590%2f0001-
3765202020191584&partnerID=40&md5=1687ed86cf418dd38df5a6346d72e8f5
VL - 92
ID - 5291
ER -
TY - JOUR
AB - In this work, the natural latex extracted from Harconia speciosa was
incorporated with silver nanoparticles (AgNP) to compose a functional biomaterial
associating the intrinsic angiogenic activity of the latex and the antimicrobial
activity of AgNP. Tissue reaction after subcutaneous implantation in dorsum of rats
of membranes without AgNP and with 0.05%, 0.4% AgNP was compared at 3, 7 and 25
days. No statistically significant difference in the tissue response of the
different biomaterials was observed, indicating that AgNP did not interfere with
the inflammatory reaction (p > 0.05) or with the angiogenic activity of latex.
Biomembranes were also tested against bacterial biofilm formation by Staphylococcus
aureus and the antimicrobial activity of the new biomaterial can be found with
bacteria crenation (0.05% AgNP) and no biofilm deposition (0.4% AgNP). Therefore,
this biomaterial has interesting properties for the tissue repair process and may
be feasible for future applications as dressing.
AN - WOS:000592870800001
AU - Bonete, J. M.
AU - Silva, G. D.
AU - Guidelli, E. J.
AU - Gokalves, P. J.
AU - Almeida, L. M.
AU - Baffa, O.
AU - Kinoshita, A.
C7 - e20191584
DO - 10.1590/0001-3765202020191584
IS - 4
PY - 2020
SN - 0001-3765
1678-2690
ST - Tissue reaction and anti-biofitm action of new biomaterial composed of latex
from Hancornia speciosa Gomes and silver nanoparticles
T2 - ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
TI - Tissue reaction and anti-biofitm action of new biomaterial composed of latex
from Hancornia speciosa Gomes and silver nanoparticles
VL - 92
ID - 5998
ER -
TY - JOUR
AB - Purpose: To study the influence of silver nanoparticles (AgNP) on tissue
reaction when incorporated into a polymeric matrix of polyacrylic acid-based
(Carbopol (R)) gel as a proposal for a new low-cost type of biomaterial that is
simple to manufacture for use as an antimicrobial and antioxidative dressing.
Methods: In-vivo tests of implantation in the subcutaneous tissue of the back of
rats were performed using polyethylene tubes in three situations: empty, only the
gel, and gel incorporated with AgNP. Then, the tissue reaction was studied by
counting inflammatory cells. Additionally, in-vitro tests of the antioxidative and
antimicrobial activity of AgNP were performed. The radical 2,2 diphenyl-1
picrylhydrazyl (DPPH) was used to test the antioxidative activity of AgNP using
electron spin resonance. The antimicrobial activity of AgNP was determined by
minimum inhibitory concentration against the microorganisms: Staphylococcus aureus,
Pseudomonas aeruginosa, and Escherichia coli. Results: The results indicated that
AgNP presents antioxidative activity and was able to inhibit the growth of the
microorganisms tested. The addition of AgNP in Carbopol (R) did not alter the
tissue inflammatory response (p>0.05, Kruskal-Wallis's test). Conclusion: The new
biomaterial is promising for future use as a dressing for its beneficial properties
for regenerative processes.
AN - WOS:000842023700001
AU - Bonete, J. M.
AU - Tamashiro, J. R.
AU - de Paiva, F. F. G.
AU - de Queiroz-Fernandes, G. M.
AU - Guidelli, E.
AU - Baffa, O.
AU - Kinoshita, A.
C7 - e370504
DO - 10.1590/acb370504
IS - 5
PY - 2022
SN - 0102-8650
1678-2674
ST - Influence of silver nanoparticles on the tissue reaction of polyacrylic acid-
based gel
T2 - ACTA CIRURGICA BRASILEIRA
TI - Influence of silver nanoparticles on the tissue reaction of polyacrylic acid-
based gel
VL - 37
ID - 5995
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs), silver oxide nanoparticles (AgO-NPs), and zinc
oxide nanoparticles (ZnO-NPs) have healing, antibacterial, and antioxidant
properties. Furthermore, Ag-NPs and ZnO-NPs also have anti-inflammatory properties.
In this study, we synthesized a nanocomposite using Ag-ZnO and AgO-NPs (Ag-ZnO/AgO
NPs). The structural and morphological properties of nanocrystals and nanocomposite
were investigated by X-ray diffraction and scanning electronics microscopic. The
wurtzite crystalline structure of Ag-ZnO and two morphologies for the nanocomposite
(nanorods and nanoplatelets) were determined. Topical treatment with 1% Ag-ZnO/AgO
NPs was compared to untreated wounds (control group). Wounds were induced in the
dorsal region of BALB/c mice and evaluated after 3, 7, 14, and 21 days of
treatment. The nanocomposite demonstrated anti-inflammatory and antioxidant
capacities. In addition, wounds treated with Ag-ZnO/AgO NPs showed accelerated
closure, non-cytotoxicity, especially on keratinocytes and collagen deposition, and
increased metalloproteinases 2 and 9 activity. The nanocomposite improved healing
by reducing the inflammatory process, protecting tissues from damage caused by free
radicals, and increasing collagen deposition in the extracellular matrix. These
characteristics contributed to the accelerated wound closure process. Thus, Ag-
ZnO/AgO NPs show potential for can be a strategy for topical use in formulations of
new drugs to treat wounds. © 2022 Elsevier B.V.
AU - Borges Rosa de Moura, F.
AU - Antonio Ferreira, B.
AU - Helena Muniz, E.
AU - Benatti Justino, A.
AU - Gabriela Silva, A.
AU - de Azambuja Ribeiro, R. I. M.
AU - Oliveira Dantas, N.
AU - Lisboa Ribeiro, D.
AU - de Assis Araújo, F.
AU - Salmen Espindola, F.
AU - Christine Almeida Silva, A.
AU - Carla Tomiosso, T.
C7 - 121620
DB - Scopus
DO - 10.1016/j.ijpharm.2022.121620
KW - Inflammation
Nanocomposite
Nanomedicine
Oxidative stress
Skin
Animals
Antioxidants
Metal Nanoparticles
Mice
Nanocomposites
Oxides
Silver
Silver Compounds
Wound Healing
Zinc Oxide
antioxidant
bovine serum albumin
C peptide
catalase
chloroform
collagen
collagen type 1
collagen type 2
collagen type 3
dimethyl sulfoxide
ferric ion
free radical
gelatinase B
hydrogel
ketamine
lanolin
malonaldehyde
metalloproteinase
methanol
n acetyl beta glucosaminidase
nanocomposite
nanocrystal
nanoparticle
nanoplatelet
nanorod
polyvinyl alcohol
pyrogallol
silver nanoparticle
silveroxide nanoparticle
syntec
thiobarbituric acid reactive substance
thiopental
tolonium chloride
trichloroacetic acid
unclassified drug
xylazine
zinc oxide
antiinfective agent
disilver oxide
metal nanoparticle
oxide
silver
silver derivative
angiogenesis
animal experiment
animal model
animal tissue
Article
cell viability
controlled study
crystal structure
cytotoxicity
densitometry
enzyme activity
epithelization
ferric reducing antioxidant power assay
HaCat cell line
histology
human
human cell
IC50
lipid peroxidation
male
mouse
MTT assay
nanomedicine
nonhuman
oxidative stress
skin defect
topical treatment
wound closure
wound healing
X ray diffraction
animal
chemistry
M3 - Article
PY - 2022
ST - Antioxidant, anti-inflammatory, and wound healing effects of topical silver-
doped zinc oxide and silver oxide nanocomposites
T2 - International Journal of Pharmaceutics
TI - Antioxidant, anti-inflammatory, and wound healing effects of topical silver-
85125480732&doi=10.1016%2fj.ijpharm.2022.121620&partnerID=40&md5=bdde42c116b210101d
28111f1f092b05
VL - 617
ID - 5144
ER -
TY - JOUR
AB - The commensal microbiota plays a fundamental role in maintaining host gut
homeostasis by controlling several metabolic, neuronal and immune functions.
Conversely, changes in the gut microenvironment may alter the saprophytic microbial
community and function, hampering the positive relationship with the host. In this
bidirectional interplay between the gut microbiota and the host, hyaluronan (HA),
an unbranched glycosaminoglycan component of the extracellular matrix, has a
multifaceted role. HA is fundamental for bacterial metabolism and influences
bacterial adhesiveness to the mucosal layer and diffusion across the epithelial
barrier. In the host, HA may be produced and distributed in different cellular
components within the gut microenvironment, playing a role in the modulation of
immune and neuronal responses. This review covers the more recent studies
highlighting the relevance of HA as a putative modulator of the communication
between luminal bacteria and the host gut neuro-immune axis both in health and
disease conditions, such as inflammatory bowel disease and ischemia/reperfusion
injury. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Bosi, A.
AU - Banfi, D.
AU - Bistoletti, M.
AU - Moretto, P.
AU - Moro, E.
AU - Crema, F.
AU - Maggi, F.
AU - Karousou, E.
AU - Viola, M.
AU - Passi, A.
AU - Vigetti, D.
AU - Giaroni, C.
AU - Baj, A.
C7 - 126
DB - Scopus
DO - 10.3390/cells11010126
IS - 1
KW - Enteric nervous system
Gastrointestinal tract
Gut microbiota
Hyaluronan
Immune system
Animals
Extracellular Matrix
Gastrointestinal Microbiome
Homeostasis
Humans
Hyaluronic Acid
Intestines
Neuroimmunomodulation
acetic acid
alpha defensin
beta defensin
butyric acid
chondroitin sulfate
claudin 2
collagen type 4
cyclooxygenase 2
dextran sulfate
entactin
fibronectin
fluorescein isothiocyanate
glial fibrillary acidic protein
glucose
glucuronic acid
glycosaminoglycan
heparin
Hermes antigen
hyaluronic acid
hyaluronic acid binding protein
hyaluronidase
immunoglobulin
inflammasome
interleukin 1
interleukin 17
interleukin 1beta
interleukin 22
interleukin 6
interleukin 8
long untranslated RNA
membrane protein
myeloid differentiation factor 88
myeloperoxidase
n acetylglucosamine
neuropeptide
neurotoxin
neurotransmitter
platelet derived growth factor receptor
polymer
polypeptide antibiotic agent
probiotic agent
propionic acid
protein ZO1
proteoglycan
reactive nitrogen species
silver nanoparticle
streptavidin
transforming growth factor beta receptor 1
uridine diphosphate glucuronic acid
abdominal pain
Akkermansia muciniphila
amino acid sequence
angiogenesis
apoptosis
arthritis
B lymphocyte
bacterial metabolism
bacterial translocation
Bacteroides
basal lamina
Bifidobacterium
binding site
bleomycin-induced lung injury
blood vessel
carbon source
cartilage
cell adhesion
cell anchorage
cell division
cell membrane
cell migration
cell proliferation
cell surface
cell vacuole
chondrocyte
chromosome 11
Citrobacter rodentium
colon
commensal
Crohn disease
cytotoxic T lymphocyte
dendritic cell
diarrhea
dietary fiber
drug metabolism
dysbiosis
elasticity
embryo development
endocytosis
Enterococcus faecium
epithelial mesenchymal transition
epithelium cell
Escherichia coli
eye surgery
Faecalibacterium
fibroblast
fibrosarcoma cell
gastrointestinal disease
gastrointestinal epithelium
gastrointestinal hemorrhage
gastrointestinal tract
gene expression
gene overexpression
hemolytic Streptococcus
homeostasis
humoral immunity
hydration
immune response
immune system
immunofluorescence
immunoreactivity
inflammatory bowel disease
intestine cell
intestine epithelium
intestine flora
intestine innervation
intestine lymphatic tissue
intestine mucosa
intestine transplantation
Lactobacillus
lamina propria
lymphocyte
macrophage
malnutrition
mast cell
microbial community
microenvironment
microvasculature
molecular weight
motor performance
multiple organ failure
natural killer cell
necrotizing enterocolitis
nerve cell
nerve cell plasticity
nervous system inflammation
neutrophil
NF kB signaling
nonhuman
osteoarthritis
oxidative stress
Paneth cell
Pasteurella multocida
phagocytosis
plasma cell
Prevotella oralis
radiosensitivity
reperfusion injury
respiratory failure
revascularization
Review
rhinopharyngitis
salmonellosis
sepsis
shock
small intestine
smooth muscle cell
Streptococcus pyogenes
superior mesenteric artery
systematic review
T lymphocyte
tissue injury
TLR signaling
tumor growth
ulcerative colitis
upregulation
vascular endothelium
vascular smooth muscle cell
wound healing
animal
human
immunomodulation
intestine
metabolism
pathology
M3 - Review
PY - 2022
ST - Hyaluronan: A neuroimmune modulator in the microbiota-gut axis
T2 - Cells
TI - Hyaluronan: A neuroimmune modulator in the microbiota-gut axis
85122023921&doi=10.3390%2fcells11010126&partnerID=40&md5=484a75b15e53e12f66da994d62
04b0fa
VL - 11
ID - 5132
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) are highlighted due to their low toxicity,
compatibility with the human body, high surface area to volume ratio, and surfaces
that can be easily modified with ligands. Biosynthesis of AuNPs using plant extract
is considered a simple, low-cost, and eco-friendly approach. Brazilian Red Propolis
(BRP), a product of bees, exhibits anti-inflammatory, anti-tumor, antioxidant, and
antimicrobial activities. Here, we described the biosynthesis of AuNPs using BRP
extract (AuNPextract) and its fractions (AuNPhexane, AuNPdichloromethane, AuNPethyl
acetate) and evaluated their structural properties and their potential against
microorganisms and cancer cells. AuNPs showed a surface plasmon resonance (SPR)
band at 535 nm. The sizes and morphologies were influenced by the BRP sample used
in the reaction. FTIR and TGA revealed the involvement of bioactive compounds from
BRP extract or its fractions in the synthesis and stabilization of AuNPs.
AuNPdichloromethane and AuNPhexane exhibited antimicrobial activities against all
strains tested, showing their efficacy as antimicrobial agents to treat infectious
diseases. AuNPs showed dose-dependent cytotoxic activity both in T24 and PC-3
cells. AuNPdichloromethane and AuNPextract exhibited the highest in vitro cytotoxic
effect. Also, the cytotoxicity of biogenic nanoparticles was induced by mechanisms
associated with apoptosis. The results highlight a potential low-cost green method
using Brazilian red propolis to synthesize AuNPs, which demonstrated significant
biological properties.
AN - WOS:000612982200117
AU - Botteon, C. E. A.
AU - Silva, L. B.
AU - Ccana-Ccapatinta, G. V.
AU - Silva, T. S.
AU - Ambrosio, S. R.
AU - Veneziani, R. C. S.
AU - Bastos, J. K.
AU - Marcato, P. D.
C7 - 1974
DA - JAN 21
DO - 10.1038/s41598-021-81281-w
IS - 1
PY - 2021
SN - 2045-2322
ST - Biosynthesis and characterization of gold nanoparticles using Brazilian red
propolis and evaluation of its antimicrobial and anticancer activities
TI - Biosynthesis and characterization of gold nanoparticles using Brazilian red
propolis and evaluation of its antimicrobial and anticancer activities
VL - 11
ID - 6499
ER -
TY - JOUR
AB - Purpose: Interstitial cystitis is a sterile bladder inflammatory disease
characterized by pelvic pain, urinary urgency and frequency. Nanocrystalline silver
has anti-inflammatory properties, prompting us to investigate its effect in
experimental bladder inflammation. Materials and Methods: Nanocrystalline silver
(0.01%, 0.05%, 0.1%, 0.5% or 1%) or phosphate buffered saline (Invitrogen (TM))
(0.5 ml) was introduced intravesically in Sprague-Dawley female rat (Charles River
Laboratories, Wilmington, Massachusetts) bladders for 20 minutes, followed by
vehicle or protamine sulfate (10 mg/ml for 30 minutes) and lipopolysaccharide
(Sigma (R)) (2 mg/ml for 45 minutes). Urine was collected. throughout for histamine
assay. The catheter was removed, the rat was returned to its cage and 4 hours later
it was sacrificed. The bladder was harvested, minced and cultured overnight. The
medium was collected for tumor necrosis factor-alpha assay. Results: Mean +/- SD
total urine histamine increased from 270 +/- 190 ng in 4 controls to 842 +/- 239 ng
after protamine sulfate/lipopolysaccharide and it decreased to 505 +/- 187 ng in 6
animals after pretreatment with 1% nanocrystalline silver (p = 0.036). Tumor
necrosis factor-a release in explant medium increased from 0.02 +/- 0.03 pg/mg in 6
controls to 0.28 +/- 0.15 pg/mg in 14 animals after treatment with protamine
sulfate/lipopolysaccharide and it decreased to 0.12 +/- 0.11 pg/mg in 10 animals
pretreated with nanocrystalline silver (p = 0.009). Nanocrystalline silver was not
effective at less than 1% and at 1% alone it released 0.05 +/- 0.07 pg/mg tumor
necrosis factor-alpha in 7 rats (vs phosphate buffered saline in 6, p = 0.387).
Nanocrystalline silver (1%) significantly decreased bladder inflammation and mast
cell activation. These effects were apparent even 4 days later. Conclusions:
Intravesical administration of nanocrystalline silver (1%) decreased urine
histamine, bladder tumor necrosis factor-a and mast cell activation without any
toxic effect. This action may be useful for interstitial cystitis.
AN - WOS:000254175000093
AU - Boucher, W.
AU - Stern, J. M.
AU - Kotsinyan, V.
AU - Kempuraj, D.
AU - Papaliodis, D.
AU - Cohen, M. S.
AU - Theoharides, T. C.
DA - APR
DO - 10.1016/j.juro.2007.11.037
IS - 4
PY - 2008
SN - 0022-5347
1527-3792
SP - 1598-1602
ST - Intravesical nanocrystalline silver decreases experimental bladder
inflammation
T2 - JOURNAL OF UROLOGY
TI - Intravesical nanocrystalline silver decreases experimental bladder
inflammation
VL - 179
ID - 6298
ER -
TY - JOUR
AB - Ingestion of engineered nanomaterials is inevitable due to their addition to
food and prevalence in food packaging and domestic products such as toothpaste and
sun cream. In the absence of robust dosimetry and particokinetic data, it is
currently challenging to accurately assess the potential toxicity of food-borne
nanomaterials. Herein, we review current understanding of gastrointestinal uptake
mechanisms, consider some data on the potential for toxicity of the most commonly
encountered classes of food-borne nanomaterials (including TiO2, SiO2 , ZnO, and Ag
nanoparticles), and discuss the potential impact of the luminal environment on
nanoparticle properties and toxicity. Much of our current understanding of
gastrointestinal nanotoxicology is derived from increasingly sophisticated
epithelial models that augment in vivo studies. In addition to considering the
direct effects of food-borne nanomaterials on gastrointestinal tissues, including
the potential role of chronic nanoparticle exposure in development of inflammatory
diseases, we also discuss the potential for food-borne nanomaterials to disturb the
normal balance of microbiota within the gastrointestinal tract. The latter
possibility warrants close attention given the increasing awareness of the critical
role of microbiota in human health and the known impact of some food-borne
nanomaterials on bacterial viability. WIREs Nanomed Nanobiotechnol 2018, 10:e1481.
doi: 10.1002/wnan.1481. This article is categorized under: Toxicology and
Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials. © 2017 The
Authors. WIREs Nanomedicine and Nanobiotechnology published by Wiley Periodicals,
Inc.
AU - Bouwmeester, H.
AU - van der Zande, M.
AU - Jepson, M. A.
C7 - e1481
DB - Scopus
DO - 10.1002/wnan.1481
IS - 1
KW - Epithelium
Food
Gastrointestinal Tract
Humans
Kinetics
Microbiota
Nanostructures
Histology
Silica
Tissue
Titanium dioxide
Toxicity
Zinc oxide
silica nanoparticle
silver nanoparticle
nanomaterial
Bacterial viability
Gastrointestinal uptakes
Inflammatory disease
Nanoparticle exposures
Nanoparticle properties
Potential impacts
dosimetry
food poisoning
gastrointestinal tissue
human
ingestion
intestine flora
kinetics
nonhuman
priority journal
Review
sedimentation
chemistry
epithelium
food
metabolism
microflora
physiology
M3 - Review
PY - 2018
ST - Effects of food-borne nanomaterials on gastrointestinal tissues and
microbiota
T2 - Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
TI - Effects of food-borne nanomaterials on gastrointestinal tissues and
microbiota
85019711368&doi=10.1002%2fwnan.1481&partnerID=40&md5=021e5de7d85ef6ec819dc027cb8a3c
59
VL - 10
ID - 5529
ER -
TY - JOUR
AB - Background: Brain microglial activations and damage responses are most
commonly associated with neurodegeneration or systemic innate immune system
activation. Here, we used histological methods to focus on microglial responses
that are directed towards brain vasculature, previously undescribed, after a
neurotoxic exposure to methamphetamine. Methods: Male rats were given doses of
methamphetamine that produce pronounced hyperthermia, hypertension, and toxicity.
Identification of microglia and microglia-like cells (pericytes and possibly
perivascular cells) was done using immunoreactivity to allograft inflammatory
factor 1 (Aif1 a.k.a Iba1) and alpha M integrin (Itgam a.k.a. Cd11b) while
vasculature endothelium was identified using rat endothelial cell antigen 1 (RECA-
1). Regions of neuronal, axonal, and nerve terminal degeneration were determined
using Fluoro-Jade C. Results: Dual labeling of vasculature (RECA-1) and microglia
(Iba1) showed a strong association of hypertrophied cells surrounding and
juxtaposed to vasculature in the septum, medial dorsal hippocampus, piriform
cortex, and thalamus. The Iba1 labeling was more pronounced in the cell body while
Cd11b more so in the processes of activated microglia. These regions have been
previously identified to have vascular leakage after neurotoxic methamphetamine
exposure. Dual labeling with Fluoro-Jade C and Iba1 indicated that there was
minimal or no evidence of neuronal damage in the septum and hippocampus where many
hypertrophied Iba1-labeled cells were found to be associated with vasculature.
Although microglial activation around the prominent neurodegeneration was found in
the thalamus, there were also many examples of activated microglia associated with
vasculature. Conclusions: The data implicate microglia, and possibly related cell
types, in playing a major role in responding to methamphetamine-induced vascular
damage, and possibly repair, in the absence of neurodegeneration. Identifying brain
regions with hypertrophied/activated microglial-like cells associated with
vasculature has the potential for identifying regions of more subtle examples of
vascular damage and BBB compromise.
AN - WOS:000371783500001
AU - Bowyer, J. F.
AU - Sarkar, S.
AU - Tranter, K. M.
AU - Hanig, J. P.
AU - Miller, D. B.
AU - O'Callaghan, J. P.
C7 - 64
DA - MAR 12
DO - 10.1186/s12974-016-0526-6
PY - 2016
SN - 1742-2094
ST - Vascular-directed responses of microglia produced by methamphetamine
exposure: indirect evidence that microglia are involved in vascular repair?
T2 - JOURNAL OF NEUROINFLAMMATION
TI - Vascular-directed responses of microglia produced by methamphetamine
exposure: indirect evidence that microglia are involved in vascular repair?
VL - 13
ID - 6831
ER -
TY - JOUR
AB - A number of studies have shown that induction of pulmonary toxicity by
nanoparticles of the same chemical composition depends on particle size, which is
likely in part due to differences in lung deposition. Particle size mostly
determines whether nanoparticles reach the alveoli, and where they might induce
toxicity. For the risk assessment of nanomaterials, there is need for a suitable
dose metric that accounts for differences in effects between different sized
nanoparticles of the same chemical composition. The aim of the present study is to
determine the most suitable dose metric to describe the effects of silver
nanoparticles after short-term inhalation. Rats were exposed to different
concentrations (ranging from 41 to 1105 μg silver/m3 air) of 18, 34, 60 and 160 nm
silver particles for four consecutive days and sacrificed at 24 h and 7 days after
exposure. We observed a concentration-dependent increase in pulmonary toxicity
parameters like cell counts and pro-inflammatory cytokines in the bronchoalveolar
lavage fluid. All results were analysed using the measured exposure concentrations
in air, the measured internal dose in the lung and the estimated alveolar dose. In
addition, we analysed the results based on mass, particle number and particle
surface area. Our study indicates that using the particle surface area as a dose
metric in the alveoli, the dose-response effects of the different silver particle
sizes overlap for most pulmonary toxicity parameters. We conclude that the alveolar
dose expressed as particle surface area is the most suitable dose metric to
describe the toxicity of silver nanoparticles after inhalation. © 2015 Informa UK
Ltd.
AU - Braakhuis, H. M.
AU - Cassee, F. R.
AU - Fokkens, P. H. B.
AU - De La Fonteyne, L. J. J.
AU - Oomen, A. G.
AU - Krystek, P.
AU - De Jong, W. H.
AU - Van Loveren, H.
AU - Park, M. V. D. Z.
DB - Scopus
DO - 10.3109/17435390.2015.1012184
IS - 1
KW - Dose metrics
inhalation exposure
nanotoxicology
risk assessment
Animals
Cytokines
Inhalation Exposure
Lung
Male
Metal Nanoparticles
Oxidative Stress
Particle Size
Pneumonia
Rats
Rats, Inbred F344
Silver
interleukin 12p70
interleukin 1beta
RANTES
silver nanoparticle
cytokine
metal nanoparticle
silver
animal experiment
animal model
Article
cell count
controlled study
dose response
dosimetry
humidity
inhalational drug administration
leukocyte differential count
lung burden
lung clearance
lung toxicity
male
nonhuman
oxidative stress
particle size
pneumonia
premature mortality
priority journal
rat
surface area
animal
chemically induced
cytology
drug effects
exposure
Fischer 344 rat
immunology
lung
metabolism
M3 - Article
PY - 2016
SP - 63-73
ST - Identification of the appropriate dose metric for pulmonary inflammation of
silver nanoparticles in an inhalation toxicity study
T2 - Nanotoxicology
TI - Identification of the appropriate dose metric for pulmonary inflammation of
silver nanoparticles in an inhalation toxicity study
84959553835&doi=10.3109%2f17435390.2015.1012184&partnerID=40&md5=7193c7b4fcb52ec837
e2ef3a2115dbb1
VL - 10
ID - 5651
ER -
TY - JOUR
AB - Background: Although silver nanoparticles are currently used in more than 400
consumer products, it is not clear to what extent they induce adverse effects after
inhalation during production and use. In this study, we determined the lung burden,
tissue distribution, and the induction and recovery of adverse effects after short-
term inhalation exposure to 15 nm and 410 nm silver nanoparticles. Methods: Rats
were nose-only exposed to clean air, 15 nm silver nanoparticles (179 μg/m3) or 410
nm silver particles (167 μg/m3) 6 hours per day, for four consecutive days. Tissue
distribution and the induction of pulmonary toxicity were determined at 24 hours
and 7 days after exposure and compared with the internal alveolar dose. Presence of
silver nanoparticles in lung cells was visualized by transmission electron
microscopy (TEM). Results: Exposure to 15 nm silver nanoparticles induced moderate
pulmonary toxicity compared to the controls, indicated by a 175-fold increased
influx of neutrophils in the lungs, a doubling of cellular damage markers in the
lungs, a 5-fold increase in pro-inflammatory cytokines, and a 1.5-fold increase in
total glutathione at 24 hours after exposure. All the observed effects disappeared
at 7 days after exposure. No effects were observed after exposure to 410 nm silver
particles. The internal alveolar mass dose of the 15 nm nanoparticles was 3.5 times
higher compared to the 410 nm particles, which equals to a 66,000 times higher
particle number. TEM analysis revealed 15 nm nanoparticles in vesicles and nuclei
of lung cells, which were decreased in size to <5 nm at 24 hours after exposure.
This demonstrates substantial dissolution of the silver nanoparticles. Conclusion:
The results show a clear size-dependent effect after inhalation of similar mass
concentrations of 15 nm and 410 nm silver (nano)particles. This can be partially
explained by the difference in the internal alveolar dose between the 15 nm and 410
nm silver (nano)particles as well as by a difference in the release rate of silver
ions. © 2014 Braakhuis et al.; licensee BioMed Central Ltd.
AU - Braakhuis, H. M.
AU - Gosens, I.
AU - Krystek, P.
AU - Boere, J. A. F.
AU - Cassee, F. R.
AU - Post, J. A.
AU - van Loveren, H.
AU - Park, M. V. D. Z.
C7 - 49
DB - Scopus
DO - 10.1186/s12989-014-0049-1
IS - 1
KW - Cellular uptake
Dissolution
Inhalation exposure
Nanoparticles
Pulmonary toxicity
Air Pollutants
Animals
Biomarkers
Cell Nucleus
Cytokines
Cytoplasmic Vesicles
Glutathione
Inhalation Exposure
Lung
Male
Metal Nanoparticles
Particle Size
Pneumonia
Random Allocation
Rats, Inbred F344
Respiratory Mucosa
Respiratory Tract Absorption
Silver
Specific Pathogen-Free Organisms
Tissue Distribution
Toxicity Tests, Acute
Toxicokinetics
cytokine
gamma interferon
glutathione
interleukin 12p70
interleukin 13
interleukin 18
interleukin 1beta
interleukin 6
macrophage inflammatory protein 1alpha
RANTES
silver nanoparticle
air pollutant
biological marker
metal nanoparticle
silver
animal cell
animal experiment
animal model
animal tissue
Article
cell count
cell damage
cell migration
cell nucleus
cell vacuole
controlled study
dissolution
histopathology
inhalation
leukocyte count
lung alveolus cell
lung lavage
lung toxicity
lymphocyte count
male
neutrophil count
nonhuman
oxidative stress
particle size
pneumonia
rat
tissue distribution
adverse effects
agonists
analysis
animal
chemically induced
chemistry
comparative study
cytoplasm vesicle
drug effects
exposure
Fischer 344 rat
germfree animal
immunology
lung
metabolism
pathology
randomization
respiratory function
respiratory tract mucosa
toxicity
toxicity testing
toxicokinetics
ultrastructure
M3 - Article
PY - 2014
ST - Particle size dependent deposition and pulmonary inflammation after short-
term inhalation of silver nanoparticles
TI - Particle size dependent deposition and pulmonary inflammation after short-
term inhalation of silver nanoparticles
84907258515&doi=10.1186%2fs12989-014-0049-
1&partnerID=40&md5=baed119ccfa08678d61e939f1f2de722
VL - 11
ID - 5570
ER -
TY - JOUR
AB - Background: Multi-organ failure (MOF) following trauma remains a significant
cause of morbidity and mortality related to a poorly understood abnormal
inflammatory response. We characterized the inflammatory response in a non-human
primate soft tissue injury and closed abdomen hemorrhage and sepsis model developed
to assess realistic injury patterns and induce MOF. Methods: Adult male Mauritan
Cynomolgus Macaques underwent laparoscopy to create a cecal perforation and non-
anatomic liver resection along with a full-thickness flank soft tissue injury.
Treatment consisted of a pre-hospital phase followed by a hospital phase after 120
minutes. Blood counts, chemistries, and cytokines/chemokines were measured
throughout the study. Lung tissue inflammation/apoptosis was confirmed by mRNA
quantitative real-time PCR (qPCR), H&E, myeloperoxidase (MPO) and TUNEL staining
was performed comparing age-matched uninjured controls to experimental animals.
Results: Twenty-one animals underwent the protocol. Mean percent hepatectomy was
64.4 +/- 5.6; percent blood loss was 69.0 +/- 12.1. Clinical evidence of end-organ
damage was reflected by a significant elevation in creatinine (1.1 +/- 0.03 vs. 1.9
+/- 0.4, p=0.026). Significant increases in systemic levels of IL-10, IL-1ra, IL-6,
G-CSF, and MCP-1 occurred (11-2986-fold) by 240 minutes. Excessive pulmonary
inflammation was evidenced by alveolar edema, congestion, and wall thickening (H&E
staining). Concordantly, amplified accumulation of MPO leukocytes and significant
pulmonary inflammation and pneumocyte apoptosis (TUNEL) was confirmed using qRT-
PCR. Conclusion: We created a clinically relevant large animal multi-trauma model
using laparoscopy that resulted in a significant systemic inflammatory response and
MOF. With this model, we anticipate studying systemic inflammation and testing
innovative therapeutic options.
AN - WOS:000414532600001
AU - Bradley, M. J.
AU - Vicente, D. A.
AU - Bograd, B. A.
AU - Sanders, E. M.
AU - Leonhardt, C. L.
AU - Elster, E. A.
AU - Davis, T. A.
C7 - 23
DA - NOV 2
DO - 10.1186/s12950-017-0170-7
PY - 2017
SN - 1476-9255
ST - Host responses to concurrent combined injuries in non-human primates
T2 - JOURNAL OF INFLAMMATION-LONDON
TI - Host responses to concurrent combined injuries in non-human primates
VL - 14
ID - 6720
ER -
TY - JOUR
AB - Consumers are orally exposed to nanoparticulate or soluble species of the
non-essential element silver due to its use in food contact materials or as a food
additive. Potential toxicity of silver nanoparticles has gained special scientific
attention. A fraction of ingested ionic or particulate silver is taken up in the
intestine and transported to the liver, where it may induce oxidative stress and
elicit subsequent adverse responses. Here, we present a comprehensive analysis of
global proteomic changes induced in human Hep G2 hepatocarcinoma cells by different
concentrations of AgPURE silver nanoparticles or by corresponding concentrations of
ionic silver. Bioinformatic analysis of proteomic data confirms and substantiates
previous findings on silver-induced alterations related to redox stress,
mitochondrial dysfunction, intermediary metabolism, inflammatory responses,
posttranslational protein modification and other cellular parameters. Similarities
between the effects exerted by the two silver species are in line with the
assumption that silver ions released from nanoparticles substantially contribute to
their toxicity. Moreover, a comparative bioinformatic evaluation of proteomic
effects in hepatic and intestinal cells exerted either by silver nanoparticles or
bionic silver is presented. Our results show that, despite remarkable differences
at the level of affected proteins in the different cell lines, highly similar
biological consequences, corresponding to previous in vivo findings, can be deduced
by applying appropriate bioinformatic data mining. Copyright © 2017 John Wiley &
Sons, Ltd.
AU - Braeuning, A.
AU - Oberemm, A.
AU - Görte, J.
AU - Böhmert, L.
AU - Juling, S.
AU - Lampen, A.
DB - Scopus
DO - 10.1002/jat.3568
IS - 5
KW - Caco-2
Hep G2
molecular toxicity
nanotoxicology
proteomics
Electrophoresis, Gel, Two-Dimensional
Hep G2 Cells
Humans
Intestines
Liver
Mass Spectrometry
Metal Nanoparticles
Proteomics
Silver Compounds
silver nanoparticle
silver nitrate
metal nanoparticle
silver derivative
Article
bioinformatics
Caco-2 cell line
comparative study
cytotoxicity
Hep-G2 cell line
human
human cell
inflammation
intestine cell
liver cell
metabolism
oxidative stress
priority journal
protein processing
redox stress
cytology
drug effect
intestine
liver
mass spectrometry
two dimensional gel electrophoresis
M3 - Article
PY - 2018
SP - 638-648
ST - Comparative proteomic analysis of silver nanoparticle effects in human liver
and intestinal cells
TI - Comparative proteomic analysis of silver nanoparticle effects in human liver
85044444604&doi=10.1002%2fjat.3568&partnerID=40&md5=b56193292b3ddc318e18fb279482360
6
VL - 38
ID - 5538
ER -
TY - JOUR
AB - Consumers are orally exposed to nanoparticulate or soluble species of the
non-essential element silver due to its use in food contact materials or as a food
additive. Potential toxicity of silver nanoparticles has gained special scientific
attention. A fraction of ingested ionic or particulate silver is taken up in the
intestine and transported to the liver, where it may induce oxidative stress and
elicit subsequent adverse responses. Here, we present a comprehensive analysis of
global proteomic changes induced in human Hep G2 hepatocarcinoma cells by different
concentrations of AgPURE silver nanoparticles or by corresponding concentrations of
ionic silver. Bioinformatic analysis of proteomic data confirms and substantiates
previous findings on silver-induced alterations related to redox stress,
mitochondrial dysfunction, intermediary metabolism, inflammatory responses,
posttranslational protein modification and other cellular parameters. Similarities
between the effects exerted by the two silver species are in line with the
assumption that silver ions released from nanoparticles substantially contribute to
their toxicity. Moreover, a comparative bioinformatic evaluation of proteomic
effects in hepatic and intestinal cells exerted either by silver nanoparticles or
bionic silver is presented. Our results show that, despite remarkable differences
at the level of affected proteins in the different cell lines, highly similar
biological consequences, corresponding to previous in vivo findings, can be deduced
by applying appropriate bioinformatic data mining. Silver nanoparticles and ionic
silver exert toxicity in cells. Consumers are orally exposed to silver from food
packaging or food additives. Here, a proteomic analysis of effects of AgPURE silver
nanoparticles and ionic silver in Hep G2 hepatocarcinoma cells is presented. Data
confirm the important role of oxidative stress in silver toxicity. A comparative
analysis of silver effects in hepatic and intestinal cells shows how bioinformatic
can help to deduce similar biological effects from remarkably differing data sets.
AN - WOS:000428416500004
AU - Braeuning, A.
AU - Oberemm, A.
AU - Gorte, J.
AU - Bohmert, L.
AU - Juling, S.
AU - Lampen, A.
DA - MAY
DO - 10.1002/jat.3568
IS - 5
PY - 2018
SN - 0260-437X
1099-1263
SP - 638-648
ST - Comparative proteomic analysis of silver nanoparticle effects in human liver
TI - Comparative proteomic analysis of silver nanoparticle effects in human liver
VL - 38
ID - 6110
ER -
TY - JOUR
AB - Oral lichen planus (OLP) is a chronic inflammatory disease with different
clinical types. Reticular and erosive forms are the most common. Although the cause
of OLP remains speculative, many findings suggest auto-immune involvement, mediated
by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma
or toxic agents can affect the epithelial homeostasia. Increased apoptosis may
cause a decrease in epithelial thickness reflecting in the activity of the lesion.
The objective of this study was to evaluate the occurrence of apoptosis and
epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each
type (reticular and erosive) plus 10 of healthy mucosa were collected and
processed. After morphometry, the apoptotic index and epitelial thickness were
obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate
the morphologic criteria used. Apoptosis in the erosive OLP was significantly more
intense than in the reticular type and both forms of OLP presented more apoptosis
than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms
and thicker in OLP reticular form than in the erosive one. The clinical differences
between reticular and erosive forms of OLP are related to variations in epithelial
thickness and in intensity of apoptosis.
O líquen plano oral (LPO) é uma doença inflamatória crônica com diferentes tipos
clínicos. As mais comuns são as formas reticular e erosiva. Embora a causa do LPO
permaneça no campo especulativo, muitos achados sugerem tratar-se de uma doença
auto-imune, mediada por linfócitos T que têm como alvo os ceratinócitos basais.
Inflamação, trauma mecânico ou agentes tóxicos podem afetar a homeostasia
epitelial. O aumento da apoptose pode levar a uma diminuição da espessura epitelial
e isto refletir na atividade da doença. O objetivo deste estudo foi avaliar a
ocorrência de apoptose e a espessura epitelial nas formas reticular e erosiva de
LPO. 15 amostras de LPO de cada tipo reticular e erosivo, além de 10 amostras de
mucosa saudável foram coletadas e processadas. Depois da morfometria, o índice
apoptótico (IA) e a espessura do epitélio foram obtidas. Reação de TUNEL e
imunohistoquímica do M30 CytoDeath foram usadas para validação dos critérios
morfológicos. A apoptose no LPO erosivo foi significativamente maior que no tipo
reticular e ambas as formas de LPO apresentaram mais apoptose que a mucosa oral
normal. A mucosa oral normal foi mais espessa que ambas as formas de LPO, sendo
que, a forma reticular foi mais espessa que o tipo erosivo. As diferenças clínicas
entre as formas reticular e erosiva de LPO têm relação com as variações na
espessura epitelial e na intensidade da apoptose.
AD - Brant, Juliana M. Caldeira
Federal University of Minas Gerais. Laboratory of Apoptosis. Departament of General
Pathology. Institute of Biological Sciences. Belo Horizonte. BR
Vasconcelos, Anilton C
Rodrigues, Luciana V
AU - Brant, Juliana M. Caldeira
AU - Vasconcelos, Anilton C.
AU - Rodrigues, Luciana V.
C1 - 20081113
DA - 2008/00
DB - LILACS
IS - 3
KW - Apoptosis
M30 CytoDEATH
Oral lichen planus
TUNEL
LA - en
PY - 2008
SN - 0103-6440
SP - 179-185
ST - Role of apoptosis in erosive and reticular oral lichen planus exhibiting
variable epithelial thickness
T2 - Braz. dent. j
TI - Role of apoptosis in erosive and reticular oral lichen planus exhibiting
variable epithelial thickness
64402008000300001
VL - 19
ID - 4952
ER -
TY - JOUR
AB - Over the years of testing biocompatibility of endodontic filling materials,
little attention has been paid to the potential adverse influences on the function
of the immune system. Therefore, the purpose of this study was to investigate the
extent to which extractable components of some commonly used root canal sealing
materials (ERCS) may interfere with immunocompetent cells in vitro. The potential
of these materials to cause delayed-type hypersensitivity (DTH) was also addressed
in a rat model system. Extractable components were drawn in cell culture medium
from freshly mixed or set material of AH 26, Grossman's sealer, Endométhasone, and
Apexit. In-vitro assays included either spleen cells or rat pulp tissue cells that
were released following enzymatic digestion with collagenase. Purified T cells for
the pulpal cell assay were obtained from rat mesenteric lymph nodes. The effect of
ERCS on the proliferation of concanavalin A (con A) stimulated spleen cell was
measured by 3H-thymidine incorporation. Pulpal accessory cell function was
monitored by the capacity of pulpal cells, pretreated with components of ERCS, to
provide signals to con A stimulated T cells. DTH was tested after subcutaneous
implantation of root canal sealers (RCS) in rats and challenge by ear injection.
Pretreatment of pulpal cells with low dilutions of eluates from extracted AH 26 and
Endomethasone resulted in a strong reduction of the T cell proliferation rate. The
effect was considerably reduced (P < 0.01) when extracts of the solid material were
employed. Extracts of Grossmans' sealer and Apexit affected T cell proliferation
only to a limited extent in the pulpal cell assay. In general, assays on spleen
cells showed a similar profile, although increased cell division was induced by
Grossman's sealer at high eluate dilutions and a concentration-dependent decrease
of cell division at lower concentrations of this material. ERCS evoked both
immunosuppression and, in some instances, immunostimulation, but they did not
release DTH.
AU - Bratel, J.
AU - Jontell, M.
AU - Dahlgren, U.
AU - Bergenholtz, G.
DB - Scopus
DO - 10.1046/j.1365-2591.1998.00148.x
IS - 3
KW - Apical periodontitis
Delayed-type hypersensitivity
Dental pulp disease
Endodontic therapy
Administration, Topical
Animals
Bismuth
Calcium Hydroxide
Cells, Cultured
Dental Pulp
Dexamethasone
Drug Combinations
Epoxy Resins
Female
Formaldehyde
Hydrocortisone
Hypersensitivity, Delayed
Immunity, Cellular
Male
Methenamine
Rats
Rats, Inbred Lew
Rats, Wistar
Silver
Spleen
T-Lymphocytes
Thymol
Titanium
ah 26 filling material
Apexit
bismuth
calcium hydroxide
corticosteroid methanetriol mixture
dexamethasone
drug derivative
epoxy resin
formaldehyde
Grossman sealer
hydrocortisone
methenamine
silver
thymol
titanium
zinc oxide eugenol
animal
article
cell culture
cellular immunity
chemically induced disorder
cytology
delayed hypersensitivity
drug combination
drug effect
female
Lewis rat
male
rat
spleen
T lymphocyte
tooth pulp
topical drug administration
Wistar rat
M3 - Article
PY - 1998
SP - 178-188
ST - Effects of root canal sealers on immunocompetent cells in vitro and in vivo
T2 - International Endodontic Journal
TI - Effects of root canal sealers on immunocompetent cells in vitro and in vivo
0032057046&doi=10.1046%2fj.1365-
2591.1998.00148.x&partnerID=40&md5=b405ead35a11e52ab1908e6f95629e83
VL - 31
ID - 5805
ER -
TY - JOUR
AB - The incorporation of engineered nanoparticles (NPs) into everyday consumer
goods, products, and applications has given rise to the field of nanotoxicology,
which evaluates the safety of NPs within biological environments. The unique
physicochemical properties of NPs have made this an insurmountable challenge, as
their reactivity and variable behavior have given rise to discrepancies between
standard cell-based in vitro and animal in vivo models. In this study, enhanced in
vitro models were generated that retained the advantages of traditional cell
cultures, but incorporated the modifications of (1) inclusion of an activated
immune element and (2) the presence of physiologically-relevant dynamic flow.
Following verification that the human alveolar epithelial and macrophage
(A549/U937) co-culture could be successfully sustained under both static and
dynamic conditions, these cultures, in addition to a standard A549 static model,
were challenged with 10 nm citrate coated silver NPs (AgNPs). This work identified
a reshaping of the AgNP-cellular interface and differential biological responses
following exposure. The presence of dynamic flow modified cellular morphology and
reduced AgNP deposition by approximately 20% over the static exposure environments.
Cellular toxicity and stress endpoints, including reactive oxygen species, heat
shock protein 70, and secretion of pro-inflammatory cytokines, were found to vary
as a function of both cellular composition and flow conditions; with activated
macrophages and fluid flow both mitigating the severity of AgNP-dependent
bioeffects. This work highlights the possibility of enhanced in vitro systems to
assess the safety of engineered NPs and demonstrates their effectiveness in
elucidating novel NP-cellular interactions and toxicological profiles. © 2021 by
the authors. Licensee MDPI, Basel, Switzerland.
AU - Braun, N. J.
AU - Galaska, R. M.
AU - Jewett, M. E.
AU - Krupa, K. A.
C7 - 1807
DB - Scopus
DO - 10.3390/nano11071807
IS - 7
KW - Cellular co-culture
Cytokine production
Dynamic flow
Nanotoxicology
Silver nanoparticle
M3 - Article
PY - 2021
ST - Implementation of a dynamic co-culture model abated silver nanoparticle
interactions and nanotoxicological outcomes in vitro
T2 - Nanomaterials
TI - Implementation of a dynamic co-culture model abated silver nanoparticle
interactions and nanotoxicological outcomes in vitro
85109542954&doi=10.3390%2fnano11071807&partnerID=40&md5=6d1e55eaa20d9f766c2b02e38a7
1194e
VL - 11
ID - 5201
ER -
TY - JOUR
AB - Many host-microbiota interactions depend on the recognition of microbial
constituents by toll-like receptors of the host. The impacts of these interactions
on host health can shape the hosts response to environmental pollutants such as
nanomaterials. Here, we assess the role of toll-like receptor 2 (TLR2) signaling in
the protective effects of colonizing microbiota against silver nanoparticle (nAg)
toxicity to zebrafish larvae. Zebrafish larvae were exposed to nAg for two days,
from 3 to 5 days post-fertilization. Using an il1ß-reporter line, we first
characterized the accumulation and particle-specific inflammatory effects of nAg in
the total body and intestinal tissues of the larvae. This showed that silver
gradually accumulated in both the total body and intestinal tissues, yet
specifically caused particle-specific inflammation on the skin of larvae.
Subsequently, we assessed the effects of microbiota-dependent TLR2 signaling on nAg
toxicity. This was done by comparing the sensitivity of loss-of-function zebrafish
mutants for TLR2, and each of the TLR2-adaptor proteins MyD88 and TIRAP (Mal),
under germ-free and microbially-colonized conditions. Irrespective of their
genotype, microbially-colonized larvae were less sensitive to nAg than their germ-
free siblings, supporting the previously identified protective effect of microbiota
against nAg toxicity. Under germ-free conditions, tlr2, myd88 and tirap mutants
were equally sensitive to nAg as their wildtype siblings. However, when colonized
by microbiota, tlr2 and tirap mutants were more sensitive to nAg than their
wildtype siblings. The sensitivity of microbially-colonized myd88 mutants did not
differ significantly from that of wildtype siblings. These results indicate that
the protective effect of colonizing microbiota against nAg-toxicity to zebrafish
larvae involves TIRAP-dependent TLR2 signaling. Overall, this supports the
conclusion that host-microbiota interactions affect nanomaterial toxicity to
zebrafish larvae. © 2022 The Authors
AU - Brinkmann, B. W.
AU - Koch, B. E. V.
AU - Peijnenburg, W. J. G. M.
AU - Vijver, M. G.
C7 - 113522
DB - Scopus
DO - 10.1016/j.ecoenv.2022.113522
KW - Host-microbiota interactions
IL1ß
Inflammation
NM-300K
Toll-like receptor
Zebrafish mutants
Animals
Larva
Metal Nanoparticles
Microbiota
Myeloid Differentiation Factor 88
Silver
Zebrafish
adaptor protein
alanine
ampicillin
interleukin 1
kanamycin
nanomaterial
silver nanoparticle
threonine
toll like receptor
tricaine
metal nanoparticle
silver
bioaccumulation
cyprinid
larva
nanoparticle
toxicity
allele
anterior intestine
Article
dispersion
fertilization
gene expression
genotype
host microbe interaction
hydrodynamics
inductively coupled plasma mass spectrometry
inflammation
intestine tissue
loss of function mutation
microflora
mortality
nonhuman
septic shock
sibling
signal transduction
zebra fish
zeta potential
animal
genetics
metabolism
M3 - Article
PY - 2022
ST - Microbiota-dependent TLR2 signaling reduces silver nanoparticle toxicity to
zebrafish larvae
T2 - Ecotoxicology and Environmental Safety
TI - Microbiota-dependent TLR2 signaling reduces silver nanoparticle toxicity to
zebrafish larvae
85128308570&doi=10.1016%2fj.ecoenv.2022.113522&partnerID=40&md5=c79faed586cac86d39b
a2a4e982b232f
VL - 237
ID - 5093
ER -
TY - JOUR
AB - ABSTRACT Aims and Objectives: Polypropylene meshes have been increasingly
adopted for correction of pelvic organ prolapse due to its lower recurrence rate
when compared to surgeries without meshes. The study of the interaction of these
materials with the host tissue may contribute to the development of materials with
best biocompatibility and, consequently, less complication rates. Materials and
Methods: The present study compares the inflammatory reaction of standard-weight
(SW) and lightweight (LW) meshes (72 g/m216g/m2 respectively), implanted in the
abdomen of 20 adult rats, which were euthanized in four or 30 days. Quantification
of pro-inflammatory markers, IL-1 and TNF-α, and of metalloproteinases, MMP2 and
MMP3, were carried out through immunohistochemistry with AxioVision® software.
Results: There were no significant differences in the quantification of IL-1 and
TNF-α in LW versus SW meshes. However, IL-1 quantification increased along time (30
days >4 days, p=0.0269). Also, MMP-2 quantification was similar to SW and LW and
both presented a significant increase along time (30 days >4 days, p <0.0001). MMP-
3 quantification also showed no difference between the SW and LW groups, but
increased along time (30 days >4 days, p=0.02). Conclusions: Mesh's density did not
influence the quantification of pro-inflammatory cytokines IL-1 and TNF-α and
metalloproteinases 2 and 3. The increased expression of IL-1, MMP-2 and MMP-3 over
time could represent a longstanding inflammatory response after PP mesh
implantation. Possibly, the occurrence of adverse events following PP prosthetic
implants can be influenced by other factors, not solely related to the amount of
implanted material.
AD - Bronzatto, Elaine
Universidade Estadual de Campinas - Unicamp. Departamento de Urologia. Campinas. BR
Riccetto, Cássio Luis Zanettini
Universidade Estadual de Campinas - Unicamp. Departamento de Urologia. Campinas. BR
AU - Bronzatto, Elaine
AU - Riccetto, Cássio Luis Zanettini
C1 - 20180808
DA - 2018/08
DB - LILACS
DO - 10.1590/s1677-5538.ibju.2016.0553
IS - 4
KW - Cytokines
Metalloproteases
Polyamide mesh [Supplementary Concept]
Polypropylenes
LA - en
PY - 2018
SN - 1677-5538
SP - 819-825
ST - Pro - inflammatory cytokines and metalloproteinase activation in
polypropylene mesh implant in rat subcutaneous tissue
T2 - Int. braz. j. urol
TI - Pro - inflammatory cytokines and metalloproteinase activation in
polypropylene mesh implant in rat subcutaneous tissue
55382018000400819
VL - 44
ID - 4922
ER -
TY - JOUR
AB - Stenotrophomonas maltophilia is an opportunistic pathogen of significant
concern to susceptible patient populations. This pathogen can cause nosoco-mial and
community-acquired respiratory and bloodstream infections and various other
infections in humans. Sources include water, plant rhizospheres, animals, and
foods. Studies of the genetic heterogeneity of S. maltophilia strains have
identified several new genogroups and suggested adaptation of this pathogen to its
habitats. The mechanisms used by S. maltophilia during pathogenesis continue to be
uncov-ered and explored. S. maltophilia virulence factors include use of motility,
biofilm forma-tion, iron acquisition mechanisms, outer membrane components, protein
secretion sys-tems, extracellular enzymes, and antimicrobial resistance mechanisms.
S. maltophilia is intrinsically drug resistant to an array of different antibiotics
and uses a broad arsenal to protect itself against antimicrobials. Surveillance
studies have recorded increases in drug resistance for S. maltophilia, prompting
new strategies to be developed against this op-portunist. The interactions of this
environmental bacterium with other microorganisms are being elucidated. S.
maltophilia and its products have applications in biotechnology, including
agriculture, biocontrol, and bioremediation. © 2021 American Society for
Microbiology. All Rights Reserved.
AU - Brooke, J. S.
C7 - e00030-19
DB - Scopus
DO - 10.1128/CMR.00030-19
IS - 3
Antimicrobial resistance
Biofilms
Biotechnology
Cystic fibrosis
Genomes
Pathogenesis
Risk factors
S. maltophilia
Stenotrophomonas
Animals
Gram-Negative Bacterial Infections
Humans
Stenotrophomonas maltophilia
Virulence Factors
abamectin
acaricide
acetylcysteine
amikacin
aminoglycoside
antibiotic agent
avibactam
azithromycin
bacteriocin
beta lactamase AmpC
beta lactamase inhibitor
bilirubin
bilirubin glucuronide
caffeic acid
carbapenem
carbapenemase
carbendazim
catalase
cefepime
cefotaxime
ceftazidime
chloramphenicol
cinnamic acid
ciprofloxacin
colistin
cotrimoxazole
cyclopeptide
deflazacort
echinocandin
elongation factor G
erythromycin
ferulic acid
flagellin
FtsZ protein
gamma interferon
gentamicin
glycosyltransferase
hyaluronic acid
hyaluronidase
imipenem
kanamycin
lactoferrin
levofloxacin
macrolide
meropenem
minocycline
moxifloxacin
mycotoxin
nanocomposite
outer membrane protein
paraquat
peptidomimetic agent
piperacillin plus tazobactam
polymyxin B
quinoline derived antiinfective agent
quinolone derivative
rifampicin
RNA 16S
ropocamptide
signal peptidase
silver nanoparticle
spiramycin
tetracycline
ticarcillin
tobramycin
triclosan
virulence factor
antiinfective agent
Actinia equina
agriculture
Anemonia sulcata
bacteremia
bacterial virulence
biofilm
bioinformatics
biological pest control
bioremediation
bloodstream infection
bone disease
coinfection
controlled study
cystic fibrosis
cytotoxicity
DNA microarray
endocarditis
endophthalmitis
enterocolitis
eye infection
febrile neutropenia
fluorescence in situ hybridization
gas chromatography
gene sequence
genetic heterogeneity
genotype
hematologic malignancy
high throughput analysis
hospital infection
human
hypoalbuminemia
immunofluorescence assay
liquid chromatography-mass spectrometry
matrix assisted laser desorption ionization time of flight mass spectrometry
meningitis
methicillin resistant Staphylococcus aureus
microbiology
multidrug resistance
multiplex polymerase chain reaction
multiplex real time polymerase chain reaction
neurologic disease
neutropenic enterocolitis
nonhuman
osteomyelitis
outer membrane
protein secretion
quantitative methylation specific polymerase chain reaction
quorum sensing
respiratory failure
respiratory system
Review
rhizosphere
risk factor
RNA sequencing
septic shock
soft tissue disease
spinal cord disease
ventilator associated pneumonia
whole genome sequencing
animal
genetics
Gram negative infection
M3 - Review
PY - 2021
ST - Advances in the microbiology of stenotrophomonas maltophilia
T2 - Clinical Microbiology Reviews
TI - Advances in the microbiology of stenotrophomonas maltophilia
85108386768&doi=10.1128%2fCMR.00030-
19&partnerID=40&md5=9129e11c4a7991498a1527e087e28d16
VL - 34
ID - 5166
ER -
TY - JOUR
AB - A major user of nanoparticles (NPs) is the pigment and ink industry, where
NPs are incorporated into numerous products (e.g. paints, food, plastics, printers,
personal care products, and construction materials). Assessment of NP toxicity
requires potential impacts on human health and the environment to be evaluated. In
this study, we examined the toxicity of a range of NPs, of varied physico-chemical
properties, used in the pigment and ink industries including silver (Ag), iron
oxide (Fe2O3), titanium dioxide (TiO2), aluminium oxide (Al2O3), zinc oxide (ZnO),
cobalt aluminium oxide (CoAl2O4) and cadmium selenide/zinc sulphide (CdSe/ZnS)
quantum dots (QDs). Acute toxicity exerted by this NP panel to mammalian cells in
vitro (macrophages, hepatocytes and alveolar epithelial cells) and aquatic
environmental organisms (Raphidocelis subcapitata Daphnia magna, Lumbriculus
variegatus) was investigated. For mammalian cells, cytotoxicity was assessed 24 h
post exposure, at concentrations ranging from 1 to 125 μg/ml using the LDH and WST-
1 assays. The aquatic toxicity of the NP panel was assessed according to OECD
protocols (201, 202, 315), up to 96 h post exposure. Rats were exposed to selected
NPs via intratracheal instillation (62 μg) and the pulmonary inflammatory response
quantified 24 h post exposure. This cross-species comparison revealed that Ag, QDs
and ZnO NPs were consistently more toxic than the other NPs tested. By looking
across mammalian and aquatic ecotoxicological models we obtained a better
understanding of the sensitivity of each model, and thus which models should be
prioritised for selection in the future when assessing the mammalian and
ecotoxicity of NPs, and in particular when screening the toxicity of a panel of
NPs. We recommend that macrophage and daphnia models are prioritised when assessing
the mammalian toxicity and ecotoxicity of NPs, respectively, due to their increased
sensitivity, compared to the other models tested. Of interest is that the in vitro
and invertebrate models used were able to predict the toxic potency of the NPs in
rodents, and thus our approach has the potential to enhance the implementation of
the 3Rs principles in nanotoxicology and reduce reliance on rodent testing when
assessing NP safety. By identifying hazardous NPs the data obtained from this study
can feed into the selection of (low toxicity) NPs to use in products and will also
contribute to the safe design of future generations of NPs used by the pigment and
ink industries. © 2018
AU - Brown, D. M.
AU - Johnston, H. J.
AU - Gaiser, B.
AU - Pinna, N.
AU - Caputo, G.
AU - Culha, M.
AU - Kelestemur, S.
AU - Altunbek, M.
AU - Stone, V.
AU - Roy, J. C.
AU - Kinross, J. H.
AU - Fernandes, T. F.
DB - Scopus
DO - 10.1016/j.impact.2018.02.001
KW - Cross-species models
In vitro
Nanoparticles
Toxicity testing
Aluminum
Aluminum compounds
Aquatic organisms
Cadmium compounds
Cell culture
Chemical industry
Cobalt compounds
Iron oxides
Macrophages
Mammals
Oxides
Plastics industry
Product design
Safety testing
Selenium compounds
Semiconductor quantum dots
Silver
Silver compounds
Sulfur compounds
Titanium dioxide
Titanium oxides
Zinc compounds
Zinc oxide
Zinc sulfide
Alveolar epithelial cells
Cross-species
Cross-species comparisons
In-vitro
Physicochemical property
Titanium dioxides (TiO2)
Toxicity
M3 - Article
PY - 2018
SP - 20-32
ST - A cross-species and model comparison of the acute toxicity of nanoparticles
used in the pigment and ink industries
T2 - NanoImpact
TI - A cross-species and model comparison of the acute toxicity of nanoparticles
used in the pigment and ink industries
85041380349&doi=10.1016%2fj.impact.2018.02.001&partnerID=40&md5=b1f42f4e720461d16e4
d6cdda4a05820
VL - 11
ID - 5532
ER -
TY - JOUR
AB - Major molecular mechanisms that underpin the toxicity of nanoparticles (NPs)
are the formation of reactive oxygen species and the induction of inflammation. The
latter is frequently observed in vitro and in mammalian organisms, yet in aquatic
organisms, such NP-induced inflammatory responses remain largely unexplored.
Zebrafish offer a wide range of molecular tools to investigate immune responses in
an aquatic organism, and were therefore used here to describe how copper (Cu) NPs
(25 nm; 1 mg L-1) and soluble Cu as well as polystyrene (PS) NPs (25 nm; 10 mg L1-)
induce innate immune responses, focussing on the skin cells and the intestine as
likely organs of interaction. mRNA expression of the immune responsive genes
interleukin 1 beta (il1 beta) and immunoresponsive gene 1-like (irg1l) of CuNP
exposed embryos was observed to be weaker in the intestinal tissue compared to the
rest of the body, indicating a strong outer epithelium response. Specifically, NPs
were observed to accumulate in the cavities of lateral neuromasts in the skin,
which coincided with an increased local expression of il1 beta. Exposure to CuNPs
triggered the strongest transcriptional changes in pro-inflammatory-related genes
and was also observed to increase migration of neutrophils in the tail, indicating
a NP-specific inflammatory response. This is the first in vivo evidence for
waterborne NP exposure triggering alterations of immune system regulating genes in
the skin and intestine of zebrafish embryos. The observed molecular responses have
the potential to be linked to adverse effects at higher levels of biological
organization and hence might be used for screening purposes in nanotoxicology or as
building blocks for adverse outcome pathways.
AN - WOS:000435963000007
AU - Brun, N. R.
AU - Koch, B. E. V.
AU - Varela, M.
AU - Peijnenburg, Wjgm
AU - Spaink, H. P.
AU - Vijver, M. G.
DA - APR 1
DO - 10.1039/c8en00002f
IS - 4
PY - 2018
SN - 2051-8153
2051-8161
SP - 904-916
ST - Nanoparticles induce dermal and intestinal innate immune system responses in
zebrafish embryos
T2 - ENVIRONMENTAL SCIENCE-NANO
TI - Nanoparticles induce dermal and intestinal innate immune system responses in
zebrafish embryos
VL - 5
ID - 6715
ER -
TY - CHAP
AB - Glioblastoma multiforme (GBM) is one of the most aggressive types of brain
tumor in humans. The prognosis for patients with GBM is unfavorable and treatment
is largely ineffective, where modern treatment regimens typically increase survival
by 15 months. GBM relapse and progression are associated with cancer stem cells
(CSCs). The present review provides a critical analysis of the primary reasons
underlying the lack of effectiveness of modern CSC management methods. An emphasis
is placed on the role of the blood-brain barrier in the development of treatment
resistance. The existing methods for increasing the efficiency of antitumor
genotoxic therapy are also described, and a strategy for personalized regulation of
CSC based on post-genome technologies is suggested. The hypothesis that GBM cells
employ a special mechanism for DNA repair based on their interactions with normal
stem cells, is presented and the function of the tumor microenvironment in
fulfilling the antitumor potential of normal stem cells is explained. Additionally,
the mechanisms by which cancer stem cells regulate glioblastoma progression and
recurrence are described based on novel biomedical technologies. © 2020 Elsevier
Inc.
AU - Bryukhovetskiy, I.
AU - Pak, O.
AU - Khotimchenko, Y.
AU - Bryukhovetskiy, A.
AU - Sharma, A.
AU - Sharma, H. S.
DB - Scopus
DO - 10.1016/bs.irn.2020.03.002
KW - Blood-brain barrier
Genotoxic therapy
Glioblastoma multiforme
Hematopoietic stem cells
Blood-Brain Barrier
Brain Neoplasms
Glioblastoma
Hematopoietic Stem Cells
Humans
Neoplastic Stem Cells
Precision Medicine
Stem Cell Niche
Tumor Microenvironment
bevacizumab
chitosan
cilengitide
cisplatin
doxorubicin
duocarmycin SA
gemcitabine
gold nanoparticle
hyaluronic acid
lomeguatrib
lomustine
macrogol
nanoparticle
olaparib
oncolytic virus
paclitaxel
polyglactin
polylactic acid
procarbazine
rindopepimut
silver nanoparticle
temozolomide
vincristine
vorinostat
blood brain barrier
brain surgery
cancer growth
cancer radiotherapy
cancer recurrence
cancer stem cell
cancer survival
cell interaction
DNA repair
drug cytotoxicity
drug dose increase
drug potentiation
drug resistance
genotoxicity
glioblastoma
human
multiple cycle treatment
nonhuman
personalized medicine
priority journal
proton therapy
stem cell transplantation
brain tumor
hematopoietic stem cell
stem cell niche
PY - 2020
SP - 67-98
ST - Personalized therapy and stem cell transplantation for pro-inflammatory
modulation of cancer stem cells microenvironment in glioblastoma: Review
T2 - International Review of Neurobiology
TI - Personalized therapy and stem cell transplantation for pro-inflammatory
modulation of cancer stem cells microenvironment in glioblastoma: Review
85084631799&doi=10.1016%2fbs.irn.2020.03.002&partnerID=40&md5=45c4dcc50084a827c17f6
b4c7ecf60d9
VL - 151
ID - 5446
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials
due to their antibacterial properties. Owing to the recent boost in the usage of
AgNPs-containing products, human exposure to AgNPs is increasing, highlighting the
need for careful evaluation of AgNPs toxicity in humans. We used two cellular
models, hepatic HepG2 and epithelial A549 cell lines, to study the mechanism of
AgNPs-induced toxicity at the cellular level. These two cell lines differ
significantly in their response to AgNPs treatment. In the case of A549 cells, a
minor decrease in viability and increase in the extent of DNA breakage were
observed. A markedly different response to AgNPs was observed in HepG2 cells. In
short term, a massive induction of DNA breakage was observed, suggesting that the
basal activity of antioxidant defence in these cells was not sufficient to
effectively protect them from the nanoparticle-induced oxidative stress. After
prolonged exposure, the extent of DNA breakage decreased to the level observed in
the control cells proving that a successful adaptation to the new conditions had
taken place. The cells that were unable to adapt must have died, as revealed by the
Neutral Red assay that indicated less than half viable cells after 24-h treatment
with 100 μg/ml of 20nm AgNPs. The gene expression analysis revealed that the
observed adaptation was underlain by a pro-proliferative, anti-apoptotic signal
leading to up-regulation of the genes promoting proliferation and inflammatory
response (EGR1, FOS, JUN, HK2, IL4, MMP10, VEGFA, WISP1, CEBPB, IL8, SELPLG), genes
coding the anti-apoptotic proteins (BCL2A1, CCL2) and factors involved in the
response to stress (HSPB1, GADD45A). Such a selection of highly resistant
population of cells should be taken into account in the case of medical
applications of nanoparticles since the sustained proliferative signalling and
resistance to cell death are hallmarks of cancer, acquired by the cells in the
process of carcinogenesis. © 2015 © The Author 2015. Published by Oxford University
Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For
permissions, please e-mail: journals.permissions@oup.com.
AU - Brzóska, K.
AU - Męczyńska-Wielgosz, S.
AU - Stępkowski, T. M.
AU - Kruszewski, M.
DB - Scopus
DO - 10.1093/mutage/gev001
IS - 3
KW - Adaptation, Physiological
Apoptosis
Cell Proliferation
Cell Survival
DNA Damage
Gene Expression
Hep G2 Cells
Humans
Metal Nanoparticles
Silver
Bcl2 related protein A1
cyclin dependent kinase inhibitor 1B
early growth response factor 1
hepatocyte nuclear factor 3beta
interleukin 4
interleukin 8
protein c jun
protein fos
silver nanoparticle
stromelysin 2
vasculotropin A
metal nanoparticle
silver
A549 cell line
apoptosis
Article
CCL2 gene
CEBPB gene
cell proliferation
cell viability
cellular stress response
controlled study
cytotoxicity
DNA strand breakage
GADD45A gene
gene
gene expression
GREB1 gene
HepG2 cell line
HK2 gene
human
human cell
inflammation
long term exposure
oxidative stress
priority journal
SELPLG gene
signal transduction
upregulation
WISP1 gene
adaptation
cell survival
DNA damage
drug effects
M3 - Article
PY - 2015
SP - 431-439
ST - Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on
the pro-proliferative and anti-apoptotic changes in gene expression
T2 - Mutagenesis
TI - Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on
84928398030&doi=10.1093%2fmutage
%2fgev001&partnerID=40&md5=dbbde1de3e0f9fae3c5fa7f28aa6b9c7
VL - 30
ID - 5644
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials
due to their antibacterial properties. Owing to the recent boost in the usage of
AgNPs-containing products, human exposure to AgNPs is increasing, highlighting the
need for careful evaluation of AgNPs toxicity in humans. We used two cellular
models, hepatic HepG2 and epithelial A549 cell lines, to study the mechanism of
AgNPs-induced toxicity at the cellular level. These two cell lines differ
significantly in their response to AgNPs treatment. In the case of A549 cells, a
minor decrease in viability and increase in the extent of DNA breakage were
observed. A markedly different response to AgNPs was observed in HepG2 cells. In
short term, a massive induction of DNA breakage was observed, suggesting that the
basal activity of antioxidant defence in these cells was not sufficient to
effectively protect them from the nanoparticle-induced oxidative stress. After
prolonged exposure, the extent of DNA breakage decreased to the level observed in
the control cells proving that a successful adaptation to the new conditions had
taken place. The cells that were unable to adapt must have died, as revealed by the
Neutral Red assay that indicated less than half viable cells after 24-h treatment
with 100 A mu g/ml of 20nm AgNPs. The gene expression analysis revealed that the
observed adaptation was underlain by a pro-proliferative, anti-apoptotic signal
leading to up-regulation of the genes promoting proliferation and inflammatory
response (EGR1, FOS, JUN, HK2, IL4, MMP10, VEGFA, WISP1, CEBPB, IL8, SELPLG), genes
coding the anti-apoptotic proteins (BCL2A1, CCL2) and factors involved in the
response to stress (HSPB1, GADD45A). Such a selection of highly resistant
population of cells should be taken into account in the case of medical
applications of nanoparticles since the sustained proliferative signalling and
resistance to cell death are hallmarks of cancer, acquired by the cells in the
process of carcinogenesis.
AN - WOS:000355317800014
AU - Brzoska, K.
AU - Meczynska-Wielgosz, S.
AU - Stepkowski, T. M.
AU - Kruszewski, M.
DA - MAY
DO - 10.1093/mutage/gev001
IS - 3
PY - 2015
SN - 0267-8357
1464-3804
SP - 431-439
ST - Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on
T2 - MUTAGENESIS
TI - Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on
VL - 30
ID - 6183
ER -
TY - JOUR
AB - Cobalt sulfate is used in the electroplating and electrochemical industries.
It is also used as a coloring agent for ceramics and as a drying agent in inks,
paints, varnishes, and linoleum. Cobalt sulfate may be added to animal feed as a
mineral supplement and has been used as a top dressing on pasture lands. Cobalt
sulfate was nominated by the National Cancer Institute for study based on a lack of
information on the toxicity of soluble salts. Male and female F344/N rats and
B6C3F1 mice were exposed to cobalt sulfate heptahydrate (approximately 99% pure) by
inhalation for 2 years. Genetic toxicology studies were conducted in Salmonella
typhimurium. The results of prechronic inhalation toxicity studies were reported
previously (Bucher et al., 1990; NTP, 1991). 2-YEAR STUDY IN RATS Groups of 50 male
and 50 female rats were exposed to aerosols containing 0, 0.3, 1.0, or 3.0 mg/m3
cobalt sulfate heptahydrate 6 hours per day, 5 days per week, for 105 weeks.
Survival and Body Weights Survival of exposed males and females was similar to that
of the chamber controls. Mean body weights of exposed male and female rats were
similar to those of the chamber controls throughout the study. Pathology Findings
The incidences and severities of proteinosis, alveolar epithelial metaplasia,
granulomatous alveolar inflammation, and interstitial fibrosis were markedly
greater in all exposed groups of male and female rats than in the chamber controls.
The incidences of alveolar epithelial hyperplasia in all groups of exposed males
and in females exposed to 3.0 mg/m3 were significantly greater than those in the
chamber control groups, as were the incidences of squamous metaplasia in 1.0 mg/m3
females and atypical alveolar epithelial hyperplasia in 3.0 mg/m3 females. In 3.0
mg/m3 males, the combined incidence of alveolar/bronchiolar neoplasms (adenoma
and/or carcinoma) was significantly greater than in the chamber controls. In female
rats exposed to 1.0 or 3.0 mg/m3, the incidences of alveolar/bronchiolar neoplasms
were significantly greater than those in the chamber control group and exceeded the
NTP historical control ranges. A squamous cell carcinoma was observed in one 1.0
mg/m3 and one 3.0 mg/m3 female. The incidences of benign, complex, or malignant
pheochromocytoma (combined) in 1.0 mg/m3 males and in 3.0 mg/m3 females were
significantly greater than those in the chamber controls and exceeded the
historical control ranges. Hyperplasia of the lateral wall of the nose, atrophy of
the olfactory epithelium, and squamous metaplasia of the epiglottis were observed
in all exposed groups of males and females, and the severities of these lesions
increased with increasing exposure concentration. The incidences of squamous
metaplasia of the lateral wall of the nose and metaplasia of the olfactory
epithelium were increased in 3.0 mg/m3 males and females. 2-YEAR STUDY IN MICE
Groups of 50 male and 50 female mice were exposed to aerosols containing 0, 0.3,
1.0, or 3.0 mg/m3 cobalt sulfate heptahydrate 6 hours per day, 5 days per week, for
105 weeks. Survival and Body Weights Survival of exposed males and females was
similar to that of the chamber controls. Mean body weights of 3.0 mg/m3 male mice
were less than those of the chamber controls from week 96 until the end of the
study. The mean body weights of all exposed groups of female mice were generally
greater than those of the chamber controls from week 20 until the end of the study.
Pathology Findings The incidences of diffuse histiocytic cell infiltration in 3.0
mg/m3 males and of focal histiocytic cell infiltration in 3.0 mg/m3 females were
significantly greater than those in the chamber controls. The incidences of
alveolar/bronchiolar neoplasms in 3.0 mg/m3 males and females were significantly
greater than those in the chamber control groups. The combined incidences of
alveolar/bronchiolar adenoma or carcinoma and the incidences of
alveolar/bronchiolar carcinoma in 3.0 mg/m3 males and females and the incidence of
alveolar/bronchiolar adenoma in 3.0 mg/m3 females exceeded the NTP historical c
ntrol ranges for inhalation studies. The incidences of atrophy of the olfactory
epithelium in 1.0 and 3.0 mg/m3 males and females and hyperplasia of the olfactory
epithelium in 3.0 mg/m3 males and females were significantly greater than in the
chamber controls. Squamous metaplasia of the larynx was observed in all exposed
groups of males and females. Male mice had a pattern of nonneoplastic liver lesions
along with silver-staining helical organisms within the liver, characteristic of an
infection with Helicobacter hepaticus. In NTP studies with H. hepaticus-associated
hepatitis, increased incidences of heman-giosarcoma were seen in the liver of male
mice. In this study of cobalt sulfate heptahydrate, incidences of hemangiosarcoma
were increased in exposed groups of male mice. Because of the above association,
interpretation of the increased incidences of hemangiosarcoma in the livers of male
mice was confounded. Incidences of lesions at other sites in this study of cobalt
sulfate heptahydrate were not considered to have been significantly impacted by the
infection with H. hepaticus or its associated hepatitis. GENETIC TOXICOLOGY Cobalt
sulfate heptahydrate was mutagenic in S. typhimurium strain TA100 with and without
liver S9 metabolic activation enzymes; no mutagenic activity was detected in strain
TA98 or TA1535, with or without S9. CONCLUSIONS Under the conditions of these 2-
year inhalation studies, there was some evidence of carcinogenic activity* of
cobalt sulfate heptahydrate in male F344/N rats based on increased incidences of
alveolar/bronchiolar neoplasms. Marginal increases in incidences of
pheochromocytomas of the adrenal medulla may have been related to exposure to
cobalt sulfate heptahydrate. There was clear evidence of carcinogenic activity in
female F344/N rats based on increased incidences of alveolar/bronchiolar neoplasms
and pheochromocytomas of the adrenal medulla in groups exposed to cobalt sulfate
heptahydrate. There was clear evidence of carcinogenic activity of cobalt sulfate
heptahydrate in male and female B6C3F1 mice based on increased incidences of
alveolar/bronchiolar neoplasms. Exposure to cobalt sulfate heptahydrate caused a
spectrum of inflammatory, fibrotic, and proliferative lesions in the respiratory
tract of male and female rats and mice. © 1998 NTP Technical Report on the
Toxicology and Carcinogenesis Studies Series. All rights reserved.
AU - Bucher, J. R.
AU - Bridge, D. A.
AU - Chapin, R. E.
AU - Hailey, J. R.
AU - Haseman, J. K.
AU - Maronpot, R. R.
AU - Rao, G. N.
AU - Roycroft, J. H.
AU - Smith, C. S.
AU - Travlos, G. S.
AU - Walters, D. B.
AU - Witt, K. L.
AU - Chou, B. J.
AU - Dill, J. A.
AU - Grumbein, S. L.
AU - Mellick, P. W.
AU - Rowe, S. E.
AU - Hardisty, J. F.
AU - Elwell, M. R.
AU - Shackelford, C. C.
AU - Brecher, S.
AU - Jokinen, M. P.
AU - Dixon, D.
AU - Everitt, J.
AU - Hahn, F. F.
AU - Herbert, R. A.
AU - Nyska, A.
AU - Radovsky, A.
AU - Morris, R. W.
AU - Lloyd, S. R.
AU - Mintz, N. G.
AU - Gunnels, S. R.
AU - Harper, L. M.
AU - Macri-Hanson, A. M.
DB - Scopus
M3 - Review
PY - 1998
SP - 1-268
ST - NTP TECHNICAL REPORT ON THE TOXICOLOGY AND CARCINOGENESIS STUDIES OF COBALT
SULFATE HEPTAHYDRATE IN F344/N RATS AND B6C3F1 MICE (INHALATION STUDIES)
T2 - NTP Technical Report on the Toxicology and Carcinogenesis Studies Series
TI - NTP TECHNICAL REPORT ON THE TOXICOLOGY AND CARCINOGENESIS STUDIES OF COBALT
SULFATE HEPTAHYDRATE IN F344/N RATS AND B6C3F1 MICE (INHALATION STUDIES)
85149251503&partnerID=40&md5=e78a2a3f4a4739f31ff39a94b8f67487
VL - TR-471
ID - 5771
ER -
TY - JOUR
AB - Three bioactive glasses belonging to the system SiO2-CaO-Na2O-P2O5 elaborated
by conventional melt-quenching techniques were doped with silver, copper and copper
+ zinc. They were characterized using the usual physical methods. Human osteoblast
cells Saos-2 and human endothelial cells EAhy926 were used for viability assays and
to assess the metallic ions, self toxicity. Human monocyte cells THP-1 were used to
measure interleukins IL1 beta and IL6 release. Glass chemical structures did not
vary much on introduction of metal ions. A layer of hydroxyapatite was observed on
every glass after 30 days of SBF immersion. A proliferative action was seen on
Saos-2 after 24 h of incubation, EAhy926 growth was not affected. For both cell
lines, a moderate cytotoxicity was found after 72 h. Dose-dependent toxic effects
of Ag, Cu and Zn ions were observed on Saos-2 and EAhy926 cells. Measured CD50 of
silver against these two cell lines were 8 to 20 fold lower than copper and zinc's.
Except undoped control glass, all doped glasses tested showed anti-inflammatory
properties by preventing IL1 beta and IL6 excretion by differentiated THP-1. In
conclusion, strictly monitored adjunction of metal ions to bioglasses ensures good
anti-inflammatory properties without altering their biocompatibility.
AN - WOS:000370057200029
AU - Bunetel, L.
AU - Ers, E. W.
AU - Novella, A.
AU - Bodin, A.
AU - Pellen-Mussi, P.
AU - Oudadesse, H.
C7 - 095402
DA - SEP
DO - 10.1088/2053-1591/2/9/095402
IS - 9
PY - 2015
SN - 2053-1591
ST - In vitro chemical and biological effects of Ag, Cu and Cu plus Zn adjunction
in 46S6 bioactive glasses
TI - In vitro chemical and biological effects of Ag, Cu and Cu plus Zn adjunction
in 46S6 bioactive glasses
VL - 2
ID - 6067
ER -
TY - JOUR
AB - Three bioactive glasses belonging to the system SiO2-CaO-Na2O-P2O5 elaborated
by conventional melt-quenching techniques were doped with silver, copper and copper
+ zinc. They were characterized using the usual physical methods. Human osteoblast
cells Saos-2 and human endothelial cells EAhy926 were used for viability assays and
to assess the metallic ions, self toxicity. Human monocyte cells THP-1 were used to
measure interleukins IL1β and IL6 release. Glass chemical structures did not vary
much on introduction of metal ions. A layer of hydroxyapatite was observed on every
glass after 30 days of SBF immersion. A proliferative action was seen on Saos-2
after 24 h of incubation, EAhy926 growth was not affected. For both cell lines, a
moderate cytotoxicity was found after 72 h. Dosedependent toxic effects of Ag, Cu
and Zn ions were observed on Saos-2 and EAhy926 cells. Measured CD50 of silver
against these two cell lines were 8 to 20 fold lower than copper and zinc's. Except
undoped control glass, all doped glasses tested showed anti-inflammatory properties
by preventing IL1β and IL6 excretion by differentiated THP-1. In conclusion,
strictly monitored adjunction of metal ions to bioglasses ensures good anti-
inflammatory properties without altering their biocompatibility. © 2015 IOP
Publishing Ltd.
AU - Bunetel, L.
AU - Wers, E.
AU - Novella, A.
AU - Bodin, A.
AU - Pellen-Mussi, P.
AU - Oudadesse, H.
C7 - 095402
DB - Scopus
DO - 10.1088/2053-1591/2/9/095402
IS - 9
KW - Anti-inflammatory properties
Bioactive glasses
Bioactivity
Biocompatibility
Metals
Bioactive glass
Cell culture
Endothelial cells
Hydroxyapatite
Metal ions
Silica
Silver
Sodium compounds
Zinc
Anti-inflammatories
Chemical and biologicals
Copper and zinc
Human endothelial cells
Human monocytes
Human osteoblast cells
Melt quenching techniques
Physical methods
Copper
M3 - Article
PY - 2015
ST - In vitro chemical and biological effects of Ag, Cu and Cu + Zn adjunction in
46S6 bioactive glasses
T2 - Materials Research Express
TI - In vitro chemical and biological effects of Ag, Cu and Cu + Zn adjunction in
46S6 bioactive glasses
84953261069&doi=10.1088%2f2053-
1591%2f2%2f9%2f095402&partnerID=40&md5=03899e00ee932b7daccdc9f310e479d8
VL - 2
ID - 5626
ER -
TY - JOUR
AB - The common effects of acetaminophen (APA), isopropylantipyrine (IPA) and
caffeine on liver were examined in rats. The preparations were given in Tween-80
solution once daily, in a constant proportion of 5:3:1, during days 8 to 14 of
gestation (S1, S2, S3 groups) or between days 8 and 14 of the experiment in
nonpregnant female Wistar rats (S1-NP, S2-NP, S3-NP groups). The administration was
in three different doses: doses S1, S1-NP - 3.5 mg/kg APA, 2.14 mg/kg IPA, 0.7
mg/kg caffeine; doses S2, S2-NP were 10 times higher; and doses S3, S3-NP 100 times
higher than doses S1, S1-NP. There were two control groups (T, T-NP) with Tween-80.
At day 21 of gestation/experiment blood was taken for determination of activity of
alanine (ALA) and aspartate (AST) aminotransferase, lactate (LDH) and glutamate
(GLDH) dehydrogenase, levels of bilirubin (BIL), urea (URE), total protein (TP) and
thymol turbidity test (TTT). The liver sections were examined by light microscopy
with four stains: hematoxylin and eosin (H+E), silver Gomori, van Gieson and
periodic acid-Schiff (PAS). There was a statistically significant (P<0.05) increase
in the GLDH (S3-NP, S2, S3 groups) and AST, LDH (S3 group) activity, a decrease in
URE (S2, S3 groups) and decrease in TP (S3-NP, S2, S3 groups) compared to the
corresponding control group. Significant differences were noted in activity of AST,
GLDH, and levels of the URE and TP between pregnant and nonpregnant females. The
treatment resulted in minimal reactive and degenerative changes in light
microscopic pattern of liver.
AU - Burdan, F.
DB - Scopus
DO - 10.1191/096032701718620873
IS - 11
KW - Acetaminophen
Caffeine
Isopropylantipyrine
Liver
Paracetamol
Pregnancy
Propyphenazone
Rat
Toxic injury
Analgesics, Non-Narcotic
Animals
Antipyrine
Body Weight
Central Nervous System Stimulants
Drug Combinations
Female
Hepatitis, Toxic
Organ Size
Periodic Acid-Schiff Reaction
Pregnancy, Animal
Rats
Rats, Wistar
Animalia
Rattus norvegicus
bilirubin
caffeine
eosin
glutamate dehydrogenase
hematoxylin
paracetamol
propyphenazone
protein
thymol
urea
animal experiment
animal model
animal tissue
article
aspartate aminotransferase blood level
blood analysis
controlled study
histopathology
laboratory test
lactate dehydrogenase blood level
liver biopsy
liver injury
liver toxicity
microscopy
nonhuman
periodic acid Schiff stain
pregnancy
priority journal
rat
statistical analysis
urea nitrogen blood level
M3 - Article
PY - 2001
SP - 569-575
ST - Combined effects of acetaminophen, isopropylantipyrine and caffeine on
pregnant and nonpregnant liver
T2 - Human and Experimental Toxicology
TI - Combined effects of acetaminophen, isopropylantipyrine and caffeine on
pregnant and nonpregnant liver
0035724974&doi=10.1191%2f096032701718620873&partnerID=40&md5=25c18a9a9f4958a9e05c55
becd7e0212
VL - 20
ID - 5815
ER -
TY - JOUR
AB - During the past few years, silver nanoparticles (AgNPs) became one of the
most investigated and explored nanotechnology-derived nanostructures, given the
fact that nanosilver-based materials proved to have interesting, challenging, and
promising characteristics suitable for various biomedical applications. Among
modern biomedical potential of AgNPs, tremendous interest is oriented toward the
therapeutically enhanced personalized healthcare practice. AgNPs proved to have
genuine features and impressive potential for the development of novel
antimicrobial agents, drug-delivery formulations, detection and diagnosis
platforms, biomaterial and medical device coatings, tissue restoration and
regeneration materials, complex healthcare condition strategies, and performance-
enhanced therapeutic alternatives. Given the impressive biomedical-related
potential applications of AgNPs, impressive efforts were undertaken on
understanding the intricate mechanisms of their biological interactions and
possible toxic effects. Within this review, we focused on the latest data regarding
the biomedical use of AgNP-based nanostructures, including aspects related to their
potential toxicity, unique physiochemical properties, and biofunctional behaviors,
discussing herein the intrinsic anti-inflammatory, antibacterial, antiviral, and
antifungal activities of silver-based nanostructures. © 2018 by the authors.
AU - Burdușel, A. C.
AU - Gherasim, O.
AU - Grumezescu, A. M.
AU - Mogoantă, L.
AU - Ficai, A.
AU - Andronescu, E.
C7 - 681
DB - Scopus
DO - 10.3390/nano8090681
IS - 9
KW - Antimicrobial efficiency
Biofunctional performances
Biological interactions
Intrinsic anti-inflammatory activity
M3 - Review
PY - 2018
ST - Biomedical applications of silver nanoparticles: An up-to-date overview
T2 - Nanomaterials
TI - Biomedical applications of silver nanoparticles: An up-to-date overview
85053250747&doi=10.3390%2fnano8090681&partnerID=40&md5=37c015b5cf6a8ae2dd5e8799d3cf
3421
VL - 8
ID - 5388
ER -
TY - JOUR
AB - During the past few years, silver nanoparticles (AgNPs) became one of the
most investigated and explored nanotechnology-derived nanostructures, given the
fact that nanosilver-based materials proved to have interesting, challenging, and
promising characteristics suitable for various biomedical applications. Among
modern biomedical potential of AgNPs, tremendous interest is oriented toward the
therapeutically enhanced personalized healthcare practice. AgNPs proved to have
genuine features and impressive potential for the development of novel
antimicrobial agents, drug-delivery formulations, detection and diagnosis
platforms, biomaterial and medical device coatings, tissue restoration and
regeneration materials, complex healthcare condition strategies, and performance-
enhanced therapeutic alternatives. Given the impressive biomedical-related
potential applications of AgNPs, impressive efforts were undertaken on
understanding the intricate mechanisms of their biological interactions and
possible toxic effects. Within this review, we focused on the latest data regarding
the biomedical use of AgNP-based nanostructures, including aspects related to their
potential toxicity, unique physiochemical properties, and biofunctional behaviors,
discussing herein the intrinsic anti-inflammatory, antibacterial, antiviral, and
antifungal activities of silver-based nanostructures.
AN - WOS:000448659200049
AU - Burdusel, A. C.
AU - Gherasim, O.
AU - Mogoanta, L.
AU - Ficai, A.
AU - Andronescu, E.
C7 - 681
DA - SEP
DO - 10.3390/nano8090681
IS - 9
PY - 2018
SN - 2079-4991
ST - Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview
T2 - NANOMATERIALS
TI - Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview
VL - 8
ID - 5876
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are being employed in numerous consumer goods
and applications; however, they are renowned for inducing negative cellular
consequences including toxicity, oxidative stress, and an inflammatory response.
Nanotoxicological outcomes are dependent on numerous factors, including
physicochemical, biological, and environmental influences. Currently, NP safety
evaluations are carried out in both cell-based in vitro and animal in vivo models,
with poor correlation between these mechanisms. These discrepancies highlight the
need for enhanced exposure environments, which retain the advantages of in vitro
models but incorporate critical in vivo influences, such as fluid dynamics. This
study characterized the effects of dynamic flow on AgNP behavior, cellular
interactions, and oxidative stress within both adherent alveolar (A549) and
suspension monocyte (U937) models. This study determined that the presence of
physiologically relevant flow resulted in substantial modifications to AgNP
cellular interactions and subsequent oxidative stress, as assessed via reactive
oxygen species (ROS), glutathione levels, p53, NFκB, and secretion of pro-
inflammatory cytokines. Within the adherent model, dynamic flow reduced AgNP
deposition and oxidative stress markers by roughly 20%. However, due to increased
frequency of contact, the suspension U937 cells were associated with higher NP
interactions and intracellular stress under fluid flow exposure conditions. For
example, the increased AgNP association resulted in a 50% increase in intracellular
ROS and p53 levels. This work highlights the potential of modified in vitro systems
to improve analysis of AgNP dosimetry and safety evaluations, including oxidative
stress assessments. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Burns, K. E.
AU - Uhrig, R. F.
AU - Jewett, M. E.
AU - Bourbon, M. F.
AU - Krupa, K. A.
C7 - 832
DB - Scopus
DO - 10.3390/antiox10060832
IS - 6
KW - Cytokine secretion
Cytotoxicity
Dynamic flow
NFκB
P53
Silver nanoparticle
M3 - Article
PY - 2021
ST - Characterizing the role of biologically relevant fluid dynamics on silver
nanoparticle dependent oxidative stress in adherent and suspension in vitro models
T2 - Antioxidants
TI - Characterizing the role of biologically relevant fluid dynamics on silver
nanoparticle dependent oxidative stress in adherent and suspension in vitro models
85106308189&doi=10.3390%2fantiox10060832&partnerID=40&md5=b8eb2b534c1c903b28dd0a479
b6ccaff
VL - 10
ID - 5241
ER -
TY - JOUR
AB - Herein, a new silver(I) complex with the non-steroidal anti-inflammatory drug
naproxen and nitrogen donor 3-picoline ligands was synthesized, characterized, and
subsequently tested for its cytotoxicity against different types of cancer cell
lines. Elemental analysis, Fourier transform infrared spectroscopy, thermal, and
proton nuclear magnetic resonance techniques showed that the molecular formula of
the prepared complex is bis(3-picoline)tris(μ-naproxenato)trisilver(I) and naproxen
ligands bind to silver ions in a bridging bidentate mode. 2,3-bis-(2-methoxy-4-
nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) results revealed that
silver salts and naproxen alone showed quite weak cytotoxic activity against human
breast adenocarcinoma (MDA-MB-453), lung adenocarcinoma (A-549), and colorectal
adenocarcinoma (HT-29) cell lines (IC50 > 250 µM), whereas the complex displayed
dose dependent cytotoxicity against the aforementioned cell lines. The highest
cytotoxicity was observed on MDA-MB-453 cells with an IC50 value of 11.73 µM.
Moreover, the complex showed higher selectivity against the cancer cell lines
compared to fibroblast 3T3-L1 cells. This study provides preliminary scientific
data on the complex for further elucidation of its anticancer mechanism of action.
© 2021
AU - Caglar, S.
AU - Altay, A.
AU - Harurluoğlu, B.
AU - Caglar, B.
DB - Scopus
DO - 10.20450/MJCCE.2021.2414
IS - 2
KW - 3-picoline
cell culture
cytotoxicity
naproxen
Silver(I) complex
M3 - Article
PY - 2021
SP - 171-180
ST - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
NAPROXEN: SYNTHESIS, CHARACTERIZATION, AND IN VITRO CYTOTOXICITY
T2 - Macedonian Journal of Chemistry and Chemical Engineering
TI - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
85123805078&doi=10.20450%2fMJCCE.2021.2414&partnerID=40&md5=69aaf13bce6a141e1b9227d
bb15ab1fc
VL - 40
ID - 5180
ER -
TY - JOUR
AB - Herein, a new silver(I) complex with the non-steroidal anti-inflammatory drug
naproxen and nitrogen donor 3-picoline ligands was synthesized, characterized, and
subsequently tested for its cytotoxicity against different types of cancer cell
lines. Elemental analysis, Fourier transform infrared spectroscopy, thermal, and
proton nuclear magnetic resonance techniques showed that the molecular formula of
the prepared complex is bis(3-picoline)tris(mu-naproxenato)trisilver(I) and
naproxen ligands bind to silver ions in a bridging bidentate mode. 2,3-bis-(2-
methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) results
revealed that silver salts and naproxen alone showed quite weak cytotoxic activity
against human breast adenocarcinoma (MDA-MB-453), lung adenocarcinoma (A-549), and
colorectal adenocarcinoma (HT-29) cell lines (IC50 > 250 mu M), whereas the complex
displayed dose dependent cytotoxicity against the aforementioned cell lines. The
highest cytotoxicity was observed on MDA-MB-453 cells with an IC50 value of 11.73
mu M. Moreover, the complex showed higher selectivity against the cancer cell lines
compared to fibroblast 3T3-L1 cells. This study provides preliminary scientific
data on the complex for further elucidation of its anticancer mechanism of action.
AN - WOS:000747339100001
AU - Caglar, S.
AU - Altay, A.
AU - Harurluoglu, B.
AU - Caglar, B.
DO - 10.20450/mjcce.2021.2414
IS - 2
PY - 2021
SN - 1857-5552
1857-5625
SP - 171-180
ST - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
T2 - MACEDONIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING
TI - TRINUCLEAR SILVER(I) COMPLEX OF NON-STEROIDAL ANTI-INFLAMMATORY DRUG
VL - 40
ID - 5899
ER -
TY - JOUR
AB - Herein, we report the synthesis, characterization and anticancer activity of
six novel complexes of non-steroidal anti-inflammatory drug niflumic acid with
Co(II) and Ni(II). In vitro cytotoxicity screening in MCF-7, HepG2 and HT-29 cancer
cell lines showed that the complex 3 [Co(nif)(2)(met)(4-pic)] and complex 6
[Ni(nif)(2)(met)(4-pic)] among all the complexes exhibited the highest cytotoxicity
against MCF-7 cells with IC50 values of 11.14 mu M and, 41.47 mu M, respectively.
Besides, all the complexes exhibited significantly higher selectivity towards mouse
fibroblast 3T3L1 cells. Further mechanistic studies with both complexes on MCF-7
cells revealed their cytotoxic action through the mitochondrial-dependent apoptotic
pathway causing an increase oxidative/nitrosative stress, decrease in mitochondrial
membrane potential (Delta psi m), inducing the multicaspase activation and
arresting the cell cycle at S phase. q-PCR analysis resulted in an increase in the
expression of the apoptotic marker proteins bax, p53 and caspase-3 and -8 in MCF-7
cells, but a decrease in the expression of antiapoptotic bcl-2 gene. Moreover, both
complexes induced the apoptosis through the inhibition of PI3K/Akt signaling
pathway by decreasing the expression of PI3K and increasing dephosphorylation form
of Akt protein. These results provide a significant contribution to the explanation
of the anticancer mechanisms of these complexes in MCF-7 cells.
AN - WOS:000645503500001
AU - Caglar, S.
AU - Altay, A.
AU - Kuzucu, M.
AU - Caglar, B.
C6 - APR 2021
DA - DEC
DO - 10.1007/s12013-021-00984-z
IS - 4
PY - 2021
SN - 1085-9195
1559-0283
SP - 729-746
ST - In Vitro Anticancer Activity of Novel Co(II) and Ni(II) Complexes of Non-
steroidal Anti-inflammatory Drug Niflumic Acid Against Human Breast Adenocarcinoma
MCF-7 Cells
T2 - CELL BIOCHEMISTRY AND BIOPHYSICS
TI - In Vitro Anticancer Activity of Novel Co(II) and Ni(II) Complexes of Non-
steroidal Anti-inflammatory Drug Niflumic Acid Against Human Breast Adenocarcinoma
MCF-7 Cells
VL - 79
ID - 6170
ER -
TY - JOUR
AB - Immune responses are involved in the pathogenesis of many diseases, including
cancer, autoimmune diseases, and chronic inflammation. These responses are
attributed to immune cells that produce cytokines, mediate cytotoxicity, and
synthesize antibodies. Gold nanoparticles (GNPs) are novel agents that intervene
with immune responses because of their unique physical-chemical properties. In
particular, GNPs enhance anti-tumour activity during immunotherapy and eliminate
excessive inflammation in autoimmune diseases. However, GNPs synthesized by
conventional methods are toxic to living organisms. Green biosynthesis provides a
safe and eco-friendly method to obtain GNPs from microbes or plant extracts. In
this review, we describe several patterns for green GNP biosynthesis. The
applications of GNPs to target immune cells and modulate the immune response are
summarized. In particular, we elaborate on how GNPs regulate innate immunity and
adaptive immunity, including inflammatory signaling and immune cell
differentiation. Finally, perspectives and challenges in utilizing green
biosynthesized GNPs for novel therapeutic approaches are discussed.
AN - WOS:000679672600001
AU - Cai, F. Y.
AU - Li, S. Y.
AU - Huang, H.
AU - Iqbal, J.
AU - Wang, C. R.
AU - Jiang, X.
C6 - JUL 2021
DA - 2021 JUL 31
DO - 10.1002/jbm.a.37281
PY - 2021
SN - 1549-3296
1552-4965
ST - Green synthesis of gold nanoparticles for immune response regulation:
Mechanisms, applications, and perspectives
T2 - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
TI - Green synthesis of gold nanoparticles for immune response regulation:
Mechanisms, applications, and perspectives
ID - 6742
ER -
TY - JOUR
AB - Design of micro- or nanocarriers for drug delivery has primarily been focused
on properties such as hydrophobicity, biodegradability, size, shape, surface
charge, and toxicity, so that they can achieve optimal delivery with respect to
drug loading, release kinetics, biodistribution, cellular uptake, and
biocompatibility. Incorporation of stimulus-sensitive moieties into the carriers
would lead to “smart” delivery systems. A further evolution would be to endow the
carrier with a therapeutic function such that it no longer serves as a mere passive
entity to release the drug at the target tissue but can be viewed as a therapeutic
agent in itself. In this review, we will discuss recent and ongoing efforts over
the past decade to design therapeutic drug carriers that confer a biological
benefit, including ROS scavenging or generating, pro- or anti-inflammatory, and
immuno-evasive properties, to enhance the overall therapeutic efficacy of the
delivery systems. © 2021 Elsevier B.V.
AU - Cai, S. S.
AU - Li, T.
AU - Akinade, T.
AU - Zhu, Y.
AU - Leong, K. W.
C7 - 113884
DB - Scopus
DO - 10.1016/j.addr.2021.113884
KW - Anti-bacterial
Anti-fibrotic
Anti-inflammatory
Anti-microbial
Drug delivery
Immunoadjuvant
ROS generation
ROS scavenging
Therapeutic carriers
Animals
Drug Delivery Systems
Humans
Biocompatibility
Biodegradability
Biology
chitosan
dendrimer
gold nanoparticle
hyaluronic acid
manganese
polyglactin
silver nanoparticle
Anti-inflammatories
Delivery systems
Drug delivery carrier
Stimulus-sensitive
Therapeutic agents
Therapeutic drugs
Therapeutic efficacy
Therapeutic functions
Alzheimer disease
apoptosis
autoimmune disease
biosafety
cancer chemotherapy
cancer radiotherapy
cytotoxicity
drug mechanism
immunomodulation
micelle
molecular weight
nanocatalyst
nanofabrication
nonhuman
oxidative stress
particle size
photothermal therapy
Review
signal transduction
animal
human
M3 - Review
PY - 2021
ST - Drug delivery carriers with therapeutic functions
T2 - Advanced Drug Delivery Reviews
TI - Drug delivery carriers with therapeutic functions
85111542782&doi=10.1016%2fj.addr.2021.113884&partnerID=40&md5=8f2121fa126842a2993a2
3e290d095ed
VL - 176
ID - 5250
ER -
TY - JOUR
AB - Few studies were made regarding the pulmonary effects of exposure to
volcanogenic air pollution, representing an unrecognized health risk for humans
inhabiting non-eruptive volcanically active areas (10% of world human population).
We tested the hypothesis whether chronic exposure to air pollution of volcanogenic
origin causes lung injury, using wild mice (Mus musculus) as model. Lung injury was
determined using histological morphometric parameters, inflammatory status (InfS)
and the amount of black silver deposits (BSD). Mice exposed to volcanogenic air
pollution have decreased percentage of alveolar space, alveolar perimeter and lung
structural functionality (LSF) ratio and, increased alveolar septal thickness,
amount of BSD and InfS. For the first time it is evidenced that non-eruptive active
volcanism has a high potential to cause lung injury. This study also highlights the
usefulness of M. musculus as bioindicator species, and of the developed biomarker
of effect LSF ratio, for future animal and/or human biomonitoring programs. © 2013
Elsevier Ltd. All rights reserved.
AU - Camarinho, R.
AU - Garcia, P. V.
AU - Rodrigues, A. S.
DB - Scopus
DO - 10.1016/j.envpol.2013.05.052
KW - Air pollution
Lung structural functionality ratio
Mice
Pulmonary inflammation
Volcanism
Air Pollutants
Air Pollution
Animals
Environmental Monitoring
Humans
Inhalation Exposure
Lung
Lung Injury
Toxicity Tests, Chronic
Volcanic Eruptions
Animalia
Mus
Mus musculus
Biomarkers
Mammals
Silver deposits
Volcanoes
Alveolar Perimeter
Alveolar Septal Thickness
AlvP
AlvST
Black Silver Deposits
BSD
Inflammatory Status
InfS
LSF ratio
Lung Structural Functionality ratio
PAlvS
Percentage of Alveolar Space
Bioindicator species
Chronic exposure
Human biomonitoring
Morphometric parameters
Structural functionality
atmospheric pollution
bioindicator
health risk
hypothesis testing
injury
pollution exposure
respiratory disease
rodent
volcanism
air pollutant
air pollution
air pollution indicator
animal experiment
animal model
animal tissue
article
black silver deposit
histopathology
lung alveolus
lung function
lung injury
mouse
nonhuman
pneumonia
tissue structure
volcano
volcanogenic air pollution
wild animal
Biological organs
M3 - Article
PY - 2013
SP - 24-30
ST - Chronic exposure to volcanogenic air pollution as cause of lung injury
T2 - Environmental Pollution
TI - Chronic exposure to volcanogenic air pollution as cause of lung injury
84879427233&doi=10.1016%2fj.envpol.2013.05.052&partnerID=40&md5=22944d5b6c613a70ab0
2d05bd90ddfb9
VL - 181
ID - 5672
ER -
TY - JOUR
AB - Recently, interest for the potential impact of consumer-relevant engineered
nanoparticles on pregnancy has dramatically increased. This study investigates
whether inhaled silver nanoparticles (AgNPs) reach and cross mouse placental
barrier and induce adverse effects. Apart from their relevance for the growing use
in consumer products and biomedical applications, AgNPs are selected since they can
be unequivocally identified in tissues. Pregnant mouse females are exposed during
the first 15 days of gestation by nose-only inhalation to a freshly produced
aerosol of 18-20 nm AgNPs for either 1 or 4 h, at a particle number concentration
of 3.80 x 10(7) part./cm(-3) and at a mass concentration of 640 mu g/m(3). AgNPs
are identified and quantitated in maternal tissues, placentas and foetuses by
transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy
and single-particle inductively coupled plasma mass spectrometry. Inhalation of
AgNPs results in increased number of resorbed foetuses associated with reduced
oestrogen plasma levels, in the 4 h/day exposed mothers. Increased expression of
pregnancy-relevant inflammatory cytokines is also detected in the placentas of both
groups. These results prove that NPs are able to reach and cross the mouse placenta
and suggest that precaution should be taken with respect to acute exposure to
nanoparticles during pregnancy.
AN - WOS:000406632700008
AU - Campagnolo, L.
AU - Massimiani, M.
AU - Vecchione, L.
AU - Piccirilli, D.
AU - Toschi, N.
AU - Magrini, A.
AU - Bonanno, E.
AU - Scimeca, M.
AU - Castagnozzi, L.
AU - Buonanno, G.
AU - Stabile, L.
AU - Cubadda, F.
AU - Aureli, F.
AU - Kreyling, W. G.
AU - Cassee, F. R.
AU - Pietroiusti, A.
DO - 10.1080/17435390.2017.1343875
IS - 5
PY - 2017
SN - 1743-5390
1743-5404
SP - 687-698
ST - Silver nanoparticles inhaled during pregnancy reach and affect the placenta
and the foetus
T2 - NANOTOXICOLOGY
TI - Silver nanoparticles inhaled during pregnancy reach and affect the placenta
and the foetus
VL - 11
ID - 6015
ER -
TY - JOUR
AB - Oxidative stress and inflammation act on skin squamous cell carcinoma (SSCC)
development and progression. Curative therapy for SSCC patients is mainly based on
surgical resection, which can cause various sequelae. Silver ions have in vitro
activities over tumor cells, while nimesulide has antioxidant and anti-inflammatory
activities. This study aimed to evaluate the effects of a silver(I) complex with
nimesulide (AgNMS) incorporated in a sustained release device based on bacterial
cellulose membrane, named AgNMS@BCM, on topic SSCC treatment. The antiproliferative
effect of AgNMS complex was evaluated in the SCC4, SCC15 and FaDu SCC lines. AgNMS
complex activity on exposure of phosphatidylserine (PS) residues and multicaspase
activation were evaluated on FaDu cells by flow cytometry. The AgNMS@BCM effects
were evaluated in a SSCC model induced by 7,12-dimethylbenzanthracene/12-o-
tetradecanoyl-phorbol-13-acetate (DMBA/TPA) in mice. Toxicity and tumor size were
evaluated throughout the study. AgNMS complex showed antiproliferative activity in
SCC15 and FaDu lines in low to moderate concentrations (67.3 mu M and 107.3 mu M,
respectively), and induced multicaspase activation on FaDu cells. The AgNMS@BCM did
not induce toxicity and reduced tumor size up to 100%. Thus, the application of
AgNMS@BCM was effective and safe in SSCC treatment in mice, and can be seen as a
potential and safe agent for topic treatment of SSCC in humans.
AN - WOS:000827406700001
AU - Candido, T. Z.
AU - de Paiva, R. E. F.
AU - Figueiredo, M. C.
AU - Coser, L. D.
AU - Frajacomo, S. C. L.
AU - Abbehausen, C.
AU - Cardinalli, I. A.
AU - Lustri, W. R.
AU - Carvalho, J. E.
AU - Ruiz, Altg
AU - Corbi, P. P.
AU - Lima, C. S. P.
C7 - 462
DA - FEB
IS - 2
PY - 2022
SN - 1999-4923
ST - Silver Nimesulide Complex in Bacterial Cellulose Membranes as an Innovative
Therapeutic Method for Topical Treatment of Skin Squamous Cell Carcinoma
T2 - PHARMACEUTICS
TI - Silver Nimesulide Complex in Bacterial Cellulose Membranes as an Innovative
VL - 14
ID - 6267
ER -
TY - JOUR
AB - Oxidative stress and inflammation act on skin squamous cell carcinoma (SSCC)
development and progression. Curative therapy for SSCC patients is mainly based on
surgical resection, which can cause various sequelae. Silver ions have in vitro
activities over tumor cells, while nime-sulide has antioxidant and anti-
inflammatory activities. This study aimed to evaluate the effects of a silver(I)
complex with nimesulide (AgNMS) incorporated in a sustained release device based on
bacterial cellulose membrane, named AgNMS@BCM, on topic SSCC treatment. The
antiproliferative effect of AgNMS complex was evaluated in the SCC4, SCC15 and FaDu
SCC lines. AgNMS complex activity on exposure of phosphatidylserine (PS) residues
and multicaspase activation were evaluated on FaDu cells by flow cytometry. The
AgNMS@BCM effects were evaluated in a SSCC model induced by 7,12-
dimethylbenzanthracene/12-o-tetradecanoyl-phorbol-13-acetate (DMBA/TPA) in mice.
Toxicity and tumor size were evaluated throughout the study. AgNMS complex showed
antiproliferative activity in SCC15 and FaDu lines in low to moderate
concentrations (67.3 µM and 107.3 µM, respectively), and induced multicaspase
activation on FaDu cells. The AgNMS@BCM did not induce toxicity and reduced tumor
size up to 100%. Thus, the application of AgNMS@BCM was effective and safe in SSCC
treatment in mice, and can be seen as a potential and safe agent for topic
treatment of SSCC in humans. © 2022 by the authors. Licensee MDPI, Basel,
Switzerland.
AU - Candido, T. Z.
AU - de Paiva, R. E. F.
AU - Figueiredo, M. C.
AU - Coser, L. O.
AU - Frajácomo, S. C. L.
AU - Abbehausen, C.
AU - Cardinalli, I. A.
AU - Lustri, W. R.
AU - Carvalho, J. E.
AU - Ruiz, A. L. T. G.
AU - Corbi, P. P.
AU - Lima, C. S. P.
C7 - 462
DB - Scopus
IS - 2
KW - Antitumor activity
Bacterial cellulose
Nimesulide
Silver
Skin carcinoma
Topical administration
cellulose
doxorubicin
nimesulide
silver nanoparticle
animal cell
animal experiment
animal model
animal tissue
Article
bacterial membrane
coating (procedure)
controlled study
drug safety
elemental analysis
flow cytometry
histopathology
human
human cell
incubation time
male
mouse
nonhuman
squamous cell skin carcinoma
tumor volume
X ray diffraction
M3 - Article
PY - 2022
ST - Silver Nimesulide Complex in Bacterial Cellulose Membranes as an Innovative
T2 - Pharmaceutics
TI - Silver Nimesulide Complex in Bacterial Cellulose Membranes as an Innovative
85125308040&doi=10.3390%2fpharmaceutics14020462&partnerID=40&md5=3f850151df8e1a6bb2
38c1b62c10e76f
VL - 14
ID - 5126
ER -
TY - JOUR
AB - With the rapid development of nanotechnologies, nanoparticles (NPs) are
increasingly produced and used in many commercial products, which could lead to the
contact of human blood vessels with NPs. Thus, it is necessary to understand the
adverse effects of NPs to relevant cells lining human blood vessels, especially
endothelial cells (ECs) that cover the lumen of blood vessels. Human umbilical vein
endothelial cells (HUVECs) are among one of the most popular models used for ECs in
vitro. In the present review, we discussed studies that have used HUVECs as a model
to investigate the EC-NP interactions, the toxic effects of NPs on ECs and the
mechanisms. The results of these studies indicated that NPs could be internalized
into HUVECs by the endocytosis pathway as well as transported across HUVECs by
exocytosis and paracellular pathways. Exposure of HUVECs to NPs could induce
cytotoxicity, genotoxicity, eNOS uncoupling and endothelial activation, which could
be explained by NP-induced oxidative stress, inflammatory response and dysfunction
of organelles. In addition, some studies have also evaluated the influences of
microenvironment (e.g. the presence of proteins and excessive nutrients), the
physiological and/or pathological stimuli related to the diversity of ECs (e.g.
shear stress, cyclic stretch and inflammatory stimuli), and the physicochemical
properties of NPs on the responses of ECs to NP exposure. In conclusion, it has
been suggested that HUVECs could be considered as a relatively reliable and simple
in vitro model for ECs to predict and evaluate the toxicity of NPs to endothelium.
Copyright (c) 2017 John Wiley & Sons, Ltd. The increasing uses of nanoparticles
(NPs) could lead to contact of human blood vessels to NPs, and it is necessary to
assess the toxicity to endothelial cells (ECs) covering blood vessels. Human
umbilical vein endothelial cells (HUVECs) are among one of the most used models for
ECs. In this review, we discuss the validation of HUVECs as a model for ECs, the
NP-HUVEC interaction, the toxic effects of NP to HUVECs, and the mechanisms and
factors that can influence the responses.
AN - WOS:000413314000001
AU - Cao, Y.
AU - Gong, Y.
AU - Liu, L. L.
AU - Zhou, Y. W.
AU - Fang, X.
AU - Zhang, C.
AU - Li, Y. N.
AU - Li, J.
DA - DEC
DO - 10.1002/jat.3470
IS - 12
PY - 2017
SN - 0260-437X
1099-1263
SP - 1359-1369
ST - The use of human umbilical vein endothelial cells (HUVECs) as an in vitro
model to assess the toxicity of nanoparticles to endothelium: a review
TI - The use of human umbilical vein endothelial cells (HUVECs) as an in vitro
model to assess the toxicity of nanoparticles to endothelium: a review
VL - 37
ID - 6320
ER -
TY - JOUR
AB - Nickel oxide nanoparticles (NiONPs) toxicity has been evaluated in the human
pulmonary epithelial cell lines: BEAS-2B and A549. The nanoparticles, used at the
doses of 20, 40, 60, 80, 100 mu g/ml, induced a significant reduction of cell
viability and an increase of apoptotic and necrotic cells at 24 h. A significant
release of interleukin-6 and -8 was assessed after 24h of treatment, even
intracellular ROS increased already at 45 min after exposure. The results obtained
evidenced that the cytokines release was dependent on mitogen activated protein
kinases (MAPK) cascade through the induction of NF-kB pathway. NiONPs induced cell
cycle alteration in both the cell lines even in different phases and these
modifications may be induced by the NPs genotoxic effect, suggested by the nuclear
translocation of phospho-ATM and phospho-ATR. Our results confirm the cytotoxic and
pro-inflammatory potential of NiONPs. Moreover their ability in inducing DNA damage
responses has been demonstrated. Such effects were present in A549 cells which
internalize the NPs and BEAS-2B cells in which endocytosis has not been observed.
(C) 2014 Elsevier Ireland Ltd. All rights reserved.
AN - WOS:000332409000005
AU - Capasso, L.
AU - Camatini, M.
AU - Gualtieri, M.
DA - APR 7
DO - 10.1016/j.toxlet.2014.01.040
IS - 1
PY - 2014
SN - 0378-4274
1879-3169
SP - 28-34
ST - Nickel oxide nanoparticles induce inflammation and genotoxic effect in lung
epithelial cells
T2 - TOXICOLOGY LETTERS
TI - Nickel oxide nanoparticles induce inflammation and genotoxic effect in lung
epithelial cells
VL - 226
ID - 6514
ER -
TY - JOUR
AB - Background and objects: Lipids with platelet activating factor (PAF)-like
activity in supernatant of packed red blood cells (PRBC) cause priming of the
neutrophil respiratory burst. This effect increases with length of storage. Washing
of PRBC has been considered as a means to eliminate this effect; however, the role
of the cellular component was not evaluated independently of the supernatant. The
source of the inflammatory lipids of the supernatant is likely to be cell membranes
altered during ageing in storage and therefore, washing will not eliminate
neutrophil priming caused by transfusion of aged PRBC units. The ability of washed
PRBC to prime mononuclear cells for another known effect of PAF, the production of
IL-8, and the probability that this lipid activity is present on microparticles in
PRBC supernatant were also investigated. Materials and methods: At collection 10
units of whole blood were split into two equal aliquots one filtered and one
unfiltered. PRBC were prepared and stored at 4 degrees C in CPD-AS5. Each week,
fresh neutrophils were incubated with samples of washed PRBC and fixed. Change in
CD11b, a marker known to increase on the surface of primed neutrophils, was
determined by flow cytometry. To determine whether neutrophil priming ability of
PRBC supernatant is contained on microvesicles, centrifuged and uncentrifuged
supernatant samples were incubated with fresh neutrophils and change in CD11b
expression was determined. Plasma IL-8 levels were also measured after exposure of
monocytes from fresh whole blood to filtered and unfiltered washed PRBC with and
without the addition of fMLP. Results: Washed PRBC caused a 50-116% increase in
CD11b neutrophil surface expression over baseline expression. Filtration of whole
blood at collection reduced this CD11b up-regulation by 25-34%. Reduction of
priming ability by filtration began on the day of collection and persisted for the
storage life of the units. Centrifugation resulted in a reduction of CD11b up-
regulation of 11-28% compared with unspun supernatant. Incubation of unfiltered
PRBC resulted in priming of mononuclear leukocytes for IL-8 production with a 73-
109% increase over baseline, but no increase over baseline was seen for incubation
with filtered blood. Conclusion: Washing does not eliminate the ability of PRBC
units to prime neutrophils and mononuclear cells, because the cellular component of
PRBC, in addition to the supernatant, induces priming. Leukodepletion filters
significantly reduce these effects compared with unfiltered PRBC. The in vitro
beneficial effect of filtration lasts for the shelf life of 42 day units. The
ability of PRBC supernatant to prime neutrophils is present on microvesicles.
Published by Elsevier Ltd.
AN - WOS:000256336800005
AU - Cardo, L. J.
AU - Wilder, D.
AU - Salata, J.
DA - APR
DO - 10.1016/j.transci.2008.01.004
IS - 2
PY - 2008
SN - 1473-0502
SP - 117-125
ST - Neutrophil priming, caused by cell membranes and microvesicles in packed red
blood cell units, is abrogated by leukocyte depletion at collection
T2 - TRANSFUSION AND APHERESIS SCIENCE
TI - Neutrophil priming, caused by cell membranes and microvesicles in packed red
blood cell units, is abrogated by leukocyte depletion at collection
VL - 38
ID - 6610
ER -
TY - JOUR
AB - The rapid advancement of nanotechnology has created a vast array of
engineered nanomaterials (ENMs) which have unique physical (size, shape,
crystallinity, surface charge) and chemical (surface coating, elemental composition
and solubility) attributes. These physicochemical properties of ENMs can produce
chemical conditions to induce a pro-oxidant environment in the cells, causing an
imbalanced cellular energy system dependent on redox potential and thereby leading
to adverse biological consequences, ranging from the initiation of inflammatory
pathways through to cell death. The present study was designed to evaluate size-
dependent cellular interactions of known biologically active silver nanoparticles
(NPs, Ag-15nm, Ag-30nm, and A-55nm). Alveolar macrophages provide the first defense
and were studied for their potential role in initiating oxidative stress. Cell
exposure produced morphologically abnormal sizes and adherence characteristics with
significant NP uptake at high doses after 24 h. Toxicity evaluations using
mitochondrial and cell membrane viability along with reactive oxygen species (ROS)
were performed. After 24 h of exposure, viability metrics significantly decreased
with increasing dose (10-75 mu g/mL-) of Ag-15nm and Ag-30nm NPs. A more than 10-
fold increase of ROS levels in cells exposed to 50 mu g/mL A-15nm suggests that the
cytotoxicity of Ag-15nm is likely to be mediated through oxidative stress. In
addition, activation of the release of traditional inflammatory mediators were
examined by measuring levels of cytokines/chemokines, including tumor necrosis
factor (TNF-alpha), macrophage inhibitory protein (MIP-2), and interleukin-6 (IL-
6), released into the culture media. After 24 h of exposure to Ag-15nm
nanoparticles, a significant inflammatory response was observed by the release of
TNF-alpha, MIP-2, and IL- 1 beta. However, there was no detectable level of IL-6
upon exposure to silver nanoparticles. In Summary, a size-dependent toxicity was
produced by silver nanoparticles, and one predominant mechanism of toxicity was
found to be largely mediated through oxidative stress.
AN - WOS:000260357700019
AU - Carlson, C.
AU - Hussain, S. M.
AU - Schrand, A. M.
AU - Braydich-Stolle, L. K.
AU - Hess, K. L.
AU - Jones, R. L.
AU - Schlager, J. J.
DA - OCT 30
DO - 10.1021/jp712087m
IS - 43
PY - 2008
SN - 1520-6106
1520-5207
SP - 13608-13619
ST - Unique Cellular Interaction of Silver Nanoparticles: Size-Dependent
Generation of Reactive Oxygen Species
T2 - JOURNAL OF PHYSICAL CHEMISTRY B
TI - Unique Cellular Interaction of Silver Nanoparticles: Size-Dependent
Generation of Reactive Oxygen Species
VL - 112
ID - 6028
ER -
TY - JOUR
AB - The rapid advancement of nanotechnology has created a vast array of
engineered nanomaterials (ENMs) which have unique physical (size, shape,
crystallinity, surface charge) and chemical (surface coating, elemental composition
and solubility) attributes. These physicochemical properties of ENMs can produce
chemical conditions to induce a pro-oxidant environment in the cells, causing an
imbalanced cellular energy system dependent on redox potential and thereby leading
to adverse biological consequences, ranging from the initiation of inflammatory
pathways through to cell death. The present study was designed to evaluate size-
dependent cellular interactions of known biologically active silver nanoparticles
(NPs, Ag-15nm, Ag-30nm, and Ag-55nm). Alveolar macrophages provide the first
defense and were studied for their potential role in initiating oxidative stress.
Cell exposure produced morphologically abnormal sizes and adherence characteristics
with significant NP uptake at high doses after 24 h. Toxicity evaluations using
mitochondrial and cell membrane viability along with reactive oxygen species (ROS)
were performed. After 24 h of exposure, viability metrics significantly decreased
with increasing dose (10-75 μg/mL) of Ag-15nm and Ag-30nm NPs. A more than 10-fold
increase of ROS levels in cells exposed to 50 μg/mL Ag-15nm suggests that the
cytotoxicity of Ag-15nm is likely to be mediated through oxidative stress. In
addition, activation of the release of traditional inflammatory mediators were
examined by measuring levels of cytokines/chemokines, including tumor necrosis
factor (TNF-a), macrophage inhibitory protein (MIP-2), and interleukin-6 (IL-6),
released into the culture media. After 24 h of exposure to Ag-15nm nanoparticles, a
significant inflammatory response was observed by the release of TNF-α, MIP-2, and
IL- 1β. However, there was no detectable level of IL-6 upon exposure to silver
nanoparticles. In summary, a size-dependent toxicity was produced by silver
nanoparticles, and one predominant mechanism of toxicity was found to be largely
mediated through oxidative stress. © 2008 American Chemical Society.
AU - Carlson, C.
AU - Hussein, S. M.
AU - Schrand, A. M.
AU - Hess, K. L.
AU - Jones, R. L.
DB - Scopus
DO - 10.1021/jp712087m
IS - 43
KW - Cell culture
Cell death
Cell membranes
Cells
Chemical properties
Cytology
Electric load management
Flow interactions
Macrophages
Nanoparticles
Nanostructures
Nuclear propulsion
Oxidative stress
Oxygen
Photolithography
Plasma waves
Theorem proving
Toxicity
Alveolar macrophages
Cell exposures
Cellular energies
Cellular interactions
Chemical conditions
Crystallinity
Culture medias
Elemental compositions
High doses
In cells
Inhibitory proteins
Physico-chemical properties
Rapid advancements
Reactive Oxygen species
Redox potentials
Surface coatings
Tumor necrosis factors
Silver
M3 - Article
PY - 2008
SP - 13608-13619
ST - Unique cellular interaction of silver nanoparticles: Size-dependent
generation of reactive oxygen species
T2 - Journal of Physical Chemistry B
TI - Unique cellular interaction of silver nanoparticles: Size-dependent
generation of reactive oxygen species
55949113520&doi=10.1021%2fjp712087m&partnerID=40&md5=e9e6b122073bfedd7ae82359cd7495
76
VL - 112
ID - 5795
ER -
TY - JOUR
AB - The indusium griseum (IG), a thin layer of gray matter in contact with the
dorsal surface of the corpus callosum and the lateral gray matter of the cingulate
gyrus, has a common origin with hippocampus and shows similar organization with the
dentate gyrus. Although some studies have examined the effect of methamphetamine
(METH), an addictive and an illegal psychostimulant on this structure, quantitative
effects and possible mechanism of actions of METH in this area are lacking. By
applying two different protocols of equivalent METH administration (i.e., a high
dose of 1 × 30 mg/kg and a lower and repeated injection dose of 3 × 10 mg/kg) and
using a specific silver staining method in mice, we demonstrate that this drug
produces degeneration in IG with both protocols, without affecting the dopaminergic
system. Moreover, we observed quantitative increases in labeling of GFAP and Iba-1,
markers of astro- and microgliosis, respectively, which suggest astrogliosis and
microgliosis. Thus, our study provides morphological and semi-quantitative evidence
that METH induces neurodegeneration in IG and that this damage is associated with
astrogliosis and microgliosis in this area. © 2014, Springer Science+Business Media
New York.
AU - Carmena, A.
AU - Granado, N.
AU - Ares-Santos, S.
AU - Alberquilla, S.
AU - Tizabi, Y.
AU - Moratalla, R.
DB - Scopus
DO - 10.1007/s12640-014-9505-9
IS - 3
KW - Astrogliosis
Indusium griseum
Methamphetamine
Microgliosis
Neurodegeneration
Silver Staining
Animals
Astrocytes
Gliosis
Limbic Lobe
Male
Mice
Mice, Inbred C57BL
Microglia
allograft inflammatory factor 1
biological marker
methamphetamine
tyrosine 3 monooxygenase
animal experiment
animal model
animal tissue
Article
astrocytosis
brain region
controlled study
corpus striatum
dopaminergic nerve cell
dopaminergic system
gliosis
hyperthermia
indusium griseum
male
mouse
nerve degeneration
nonhuman
priority journal
quantitative analysis
rectum temperature
silver staining
toxicology
animal
astrocyte
C57BL mouse
chemically induced
drug effects
limbic cortex
microglia
pathology
M3 - Article
PY - 2015
SP - 209-216
ST - Methamphetamine-Induced Toxicity in Indusium Griseum of Mice is Associated
with Astro- and Microgliosis
T2 - Neurotoxicity Research
TI - Methamphetamine-Induced Toxicity in Indusium Griseum of Mice is Associated
with Astro- and Microgliosis
84925464640&doi=10.1007%2fs12640-014-9505-
9&partnerID=40&md5=deb9739a049e4bb6a5481514ac081f17
VL - 27
ID - 5590
ER -
TY - JOUR
AB - This study employed NMR metabolomics to characterize macrophage responses to
subtoxic concentrations of silver nanoparticles (AgNPs ca. 30 nm) and ionic silver
(Ag+), with a view to further elucidate their immunomodulatory activity at the cell
metabolism level. Exposure to AgNPs caused RAW 264.7 macrophages to decrease
intracellular glucose utilization, possibly due to interference with glycolytic
enzymes, and to reprogram the TCA cycle towards anaplerotic fueling and production
of anti-inflammatory metabolites (e.g. itaconate and creatine). Moreover, AgNPs-
exposed cells were able to control the levels of reactive oxygen/nitrogen species
(ROS/RNS), likely through upregulation of glutathione synthesis. On the other hand,
macrophages exposed to Ag+ at equivalent subtoxic concentrations showed reduced
metabolic activity, lower ability to counterbalance ROS/RNS and alterations in
membrane-related lipids. Overall, the metabolomics approach hereby employed
provided novel insights into the differential effects of AgNPs and Ag+, which help
explain the lower toxic potential of nanosilver than silver ions.
AN - WOS:000530644100001
AU - Carrola, J.
AU - Bastos, V.
AU - Gil, A. M.
AU - Santos, C.
AU - Oliveira, H.
AU - Duarte, I. F.
C6 - MAY 2020
DA - MAY 22
DO - 10.1002/ejic.202000095
IS - 19
PY - 2020
SN - 1434-1948
1099-0682
SP - 1867-1876
ST - Macrophage Metabolomics Reveals Differential Metabolic Responses to Subtoxic
Levels of Silver Nanoparticles and Ionic Silver
T2 - EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
TI - Macrophage Metabolomics Reveals Differential Metabolic Responses to Subtoxic
Levels of Silver Nanoparticles and Ionic Silver
VL - 2020
ID - 5971
ER -
TY - JOUR
AB - Aims: Capped silver nanoparticles that can be coupled to a variety of
molecules and biomolecules are of great interest owing to their potential
applications in biomedicine. However, there are no data about their toxicity or
functional effects on a key innate immune response, such as IL-6 secretion, after
the engagement of the main group of pathogen-associated molecular patterns
receptors, that is, the Toll-like receptors (TLRs). Materials & methods: N-(2-
mercaptopropionyl)glycine (tiopronin)-capped silver (Agτiopronin) nanoparticles of
a narrow sized distribution (∼5 nm) were synthesized and characterized by
transmission electron microscopy, Fourier transform infrared spectroscopy, Raman,
1H-NMR and total correlation spectroscopy. Cytotoxicity was determined by lactate
deshidrogenase and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium assays in
Raw 264.7 macrophages. IL-6 was measured by ELISA. Results & discussion:
Agτiopronin nanoparticles have a narrow size distribution (≈5 nm), high solubility
and stability in aqueous environment with no cytotoxicity in terms of mitochondrial
function or plasma-membrane integrity at concentrations as high as 200 μg/106
cells. Agτiopronin nanoparticles were not proinflammatory agents, but remarkably
they specifically impaired the IL-6 secretion mediated by TLR2, TLR2/6, TLR3 or
TLR9 stimulation in co-treatment experiments. However, in pretreatment experiments,
nanoparticles enhanced the susceptibility of macrophages to inflammatory
stimulation mediated by TLR2/1 and TLR2/6 specific ligands while severely impairing
the IL-6 secretion activated by the TLR3 or TLR9 ligands. Conclusions: Contrary to
what is found for bare silver nanoparticles, Agτiopronin nanoparticles are
noncytotoxic to macrophages. Agτiopronin nanoparticles showed differential effects
on TLR signaling of a high degree of specificity, without proinflammatory effects
by themselves. These effects have to be borne in mind when using bioconjugates of
Agτiopronin nanoparticles for future medical applications. © 2008 Future Medicine
Ltd.
AU - Castillo, P. M.
AU - Herrera, J. L.
AU - Fernandez-Montesinos, R.
AU - Caro, C.
AU - Zaderenko, A. P.
AU - Mejias, J. A.
AU - Pozo, D.
DB - Scopus
DO - 10.2217/17435889.3.5.627
IS - 5
KW - Immunoregulation
Innate immunity
Macrophages
Metal nanoparticles
Tiopronin
Toll-like receptors
Toxicity
Animals
Cell Line
Interleukin-6
Nanoparticles
Silver
Thiopronine
Toll-Like Receptors
interleukin 6
metal nanoparticle
nanocoating
silver nanoparticle
tiopronin
toll like receptor
unclassified drug
animal cell
article
controlled study
cytokine release
cytotoxicity
drug coating
drug distribution
drug solubility
drug stability
immunomodulation
inflammation
innate immunity
macrophage
nonhuman
priority journal
Raman spectrometry
signal transduction
toxicity testing
M3 - Article
PY - 2008
SP - 627-635
ST - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
mediated by Toll-like receptor ligands
T2 - Nanomedicine
TI - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
56549094531&doi=10.2217%2f17435889.3.5.627&partnerID=40&md5=0535acbdaadd8aa6eca147c
04d4270ca
VL - 3
ID - 5792
ER -
TY - JOUR
AB - Aims: Capped silver nanoparticles that can be coupled to a variety of
molecules and biomolecules are of great interest owing to their potential
applications in biomedicine. However, there are no data about their toxicity or
functional effects on a key innate immune response, such as IL-6 secretion, after
the engagement of the main group of pathogen-associated molecular patterns
receptors, that is, the Toll-like receptors (TLRs). Materials & methods: N-(2-
mercaptopropionyl)glycine (tiopronin)-capped silver (Ag@tiopronin) nanoparticles of
a narrow sized distribution (similar to 5 nm) were synthesized and characterized by
transmission electron microscopy, Fourier transform infrared spectroscopy, Raman,
H-1-NMR and total correlation spectroscopy. Cytotoxicity was determined by lactate
deshidrogenase and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium assays in
Raw 264.7 macrophages. IL-6 was measured by ELISA. Results & discussion:
Ag@tiopronin nanoparticles have a narrow size distribution (approximate to 5 nm),
high solubility and stability in aqueous environment with no cytotoxicity in terms
of mitochondrial function or plasma-membrane integrity at concentrations as high as
200 mu g/10(6) cells. Ag@tiopronin nanoparticles were not proinflammatory agents,
but remarkably they specifically impaired the IL-6 secretion mediated by TLR2,
TLR2/6, TLR3 or TLR9 stimulation in co-treatment experiments. However, in
pretreatment experiments, nanoparticles enhanced the susceptibility of macrophages
to inflammatory stimulation mediated by TLR2/1 and TLR2/6 specific ligands while
severely impairing the IL-6 secretion activated by the TLR3 or TLR9 ligands.
Conclusions: Contrary to what is found for bare silver nanoparticles, Ag@tiopronin
nanoparticles are noncytotoxic to macrophages. Ag@tiopronin nanoparticles showed
differential effects on TLR signaling of a high degree of specificity, without
proinflammatory effects by themselves. These effects have to be borne in mind when
using bioconjugates of Ag@tiopronin nanoparticles for future medical applications.
AN - WOS:000260291400011
AU - Herrera, J. L.
AU - Caro, C.
AU - Mijias, J. A.
AU - Pozo, D.
DA - OCT
DO - 10.2217/17435889.3.5.627
IS - 5
PY - 2008
SN - 1743-5889
1748-6963
SP - 627-635
ST - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
T2 - NANOMEDICINE
TI - Tiopronin monolayer-protected silver nanoparticles modulate IL-6 secretion
VL - 3
ID - 6079
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are one of the most studied nanoparticles due to
their anti-bacterial, -fungal, -viral, -parasitic, and -inflammatory properties.
This raises the need to evaluate the toxicity and biological effects of AgNP in the
immune system in order to develop new safer biomedical products. In this study, an
AgNP formulation currently approved for veterinary applications was applied to
mouse bone marrow-derived dendritic cells (BMDC), considered important antigen-
presenting cells of the immune system, to evaluate cytotoxicity, genotoxicity, and
any significant influence on expression of cellular markers associated with BMDC
phenotype and maturation status. The results showed that after 12 h of AgNP
exposure, a significant decrease in BMDC viability occurred at the highest
concentration tested (1.0 µg AgNP/ml) and at lower doses, the cells maintained
membrane integrity and metabolic activity. DNA damage was not significant with any
AgNP level aside from the 1.0 µg AgNP/ml level. Regarding phenotype, no differences
in expression of CD40 (co-stimulatory molecule highly present in mature BMDC) or in
CD273 (a marker for inhibitory T-cell response) were observed. The current results
showed that the toxicity of this AgNP formulation was dose-related. The findings
also suggest BMDC could maintain structural conservation of co-stimulatory/co-
inhibitory surface molecules after 12 h of exposure to this AgNP. This work
represents the first step in identifying the toxic effects of this AgNP formulation
on dendritic cells. © 2019, © 2019 The Author(s). Published by Informa UK Limited,
trading as Taylor & Francis Group.
AU - Castro-Gamboa, S.
AU - Garcia-Garcia, M. R.
AU - Piñon-Zarate, G.
AU - Rojas-Lemus, M.
AU - Jarquin-Yañez, K.
AU - Angel Herrera-Enriquez, M.
AU - Fortoul, T. I.
AU - Toledano-Magaña, Y.
AU - Garcia-Iglesias, T.
AU - Pestryakov, A.
AU - Eliu Castell-Rodriguez, A.
AU - Bogdanchikova, N.
DB - Scopus
DO - 10.1080/1547691X.2019.1584652
IS - 1
KW - Bone marrow-derived dendritic cells
cytotoxicity
genotoxicity
immunophenotype
Animals
Bone Marrow Cells
CD40 Antigens
Dendritic Cells
DNA Damage
Male
Metal Nanoparticles
Mice
Programmed Cell Death 1 Ligand 2 Protein
Silver
CD40 antigen
cell marker
programmed death 1 ligand 2
silver nanoparticle
metal nanoparticle
silver
animal cell
antigen presenting cell
apoptosis
Article
bone marrow derived dendritic cell
cell maturation
cell viability
cellular immunity
controlled study
DNA damage
immunophenotyping
male
mouse
nanotoxicology
nonhuman
oxidative stress
primary culture
priority journal
veterinary medicine
animal
bone marrow cell
dendritic cell
immunology
pathology
M3 - Article
PY - 2019
SP - 54-62
ST - Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic
cells: Implications for phenotype
TI - Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic
85063752098&doi=10.1080%2f1547691X.2019.1584652&partnerID=40&md5=e15a0c57f1827ec9f4
c061b3281d5845
VL - 16
ID - 5430
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are one of the most studied nanoparticles due to
their anti-bacterial, -fungal, -viral, -parasitic, and -inflammatory properties.
This raises the need to evaluate the toxicity and biological effects of AgNP in the
immune system in order to develop new safer biomedical products. In this study, an
AgNP formulation currently approved for veterinary applications was applied to
mouse bone marrowderived dendritic cells (BMDC), considered important antigen-
presenting cells of the immune system, to evaluate cytotoxicity, genotoxicity, and
any significant influence on expression of cellular markers associated with BMDC
phenotype and maturation status. The results showed that after 12 h of AgNP
exposure, a significant decrease in BMDC viability occurred at the highest
concentration tested (1.0 mg AgNP/ml) and at lower doses, the cells maintained
membrane integrity and metabolic activity. DNA damage was not significant with any
AgNP level aside from the 1.0 mg AgNP/ml level. Regarding phenotype, no differences
in expression of CD40 (co-stimulatory molecule highly present in mature BMDC) or in
CD273 (a marker for inhibitory T-cell response) were observed. The current results
showed that the toxicity of this AgNP formulation was dose-related. The findings
also suggest BMDC could maintain structural conservation of co-stimulatory/co-
inhibitory surface molecules after 12 h of exposure to this AgNP. This work
represents the first step in identifying the toxic effects of this AgNP formulation
on dendritic cells.
AN - WOS:000555038000006
AU - Castro-Gamboa, S.
AU - Garcia-Garcia, M. R.
AU - Pinon-Zarate, G.
AU - Rojas-Lemus, M.
AU - Jarquin-Yanez, K.
AU - Herrera-Enriquez, M. A.
AU - Fortoul, T. I.
AU - Toledano-Magana, Y.
AU - Garcia-Iglesias, T.
AU - Pestryakov, A.
AU - Castell-Rodriguez, A. E.
AU - Bogdanchikova, N.
DO - 10.1080/1547691X.2019.1584652
IS - 1
PY - 2019
SN - 1547-691X
1547-6901
SP - 54-62
ST - Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic
T2 - JOURNAL OF IMMUNOTOXICOLOGY
TI - Toxicity of silver nanoparticles in mouse bone marrow-derived dendritic
VL - 16
ID - 5865
ER -
TY - JOUR
AB - Antibodies to Circumsporozoite protein (CSP) confer protection against
controlled human malaria infection (CHMI) caused by the parasite Plasmodium
falciparum. Although CSP is highly immunogenic, it does not induce long lasting
protection and efforts to improve CSP-specific immunological memory and duration of
protection are underway. We have previously reported that the clinical grade CSP
vaccine FMP013 was immunogenic and protective against malaria challenge in mice
when combined with the Army Liposomal Formulation adjuvant containing immune
modulators 3D-PHAD (TM) and QS21 (ALFQ). To move forward with clinical evaluation,
we now report the safety, toxicity and immunogenicity of clinical grade FMP013 and
ALFQ in Rhesus macaques. Three groups of Rhesus (n = 6) received half or full human
dose of FMP013 + ALFQ on a 0-1-2 month schedule, which showed mild local site
reactions with no hematologic derangements in red blood cell homeostasis, liver
function or kidney function. Immunization induced a transient systemic inflammatory
response, including elevated white blood cell counts, mild fever, and a few
incidences of elevated creatine kinase, receding to normal range by day 7 post
vaccination. Optimal immunogenicity in Rhesus was observed using a 1 mL ALFQ+ 20 mu
g FMP013 dose. Doubling the FMP013 antigen dose to 40 mu g had no effect while
halving the ALFQ adjuvant dose to 0.5 mL lowered immunogenicity. Similar to data
generated in mice, FMP013 + ALFQ induced serum antibodies that reacted to all
regions of the CSP molecule and a Th1-biased cytokine response in Rhesus. Rhesus
antibody response to FMP013 + ALFQ was found to be non-inferior to historical
benchmarks including that of RTS,S + AS01 in humans. A four-dose GLP toxicity study
in rabbits confirmed no local site reactions and transient systemic inflammation
associated with ALFQ adjuvant administration. These safety and immunogenicity data
support the clinical progression and testing of FMP013 + ALFQ in a CHMI trial in
the near future. (C) 2019 The Author(s). Published by Elsevier Ltd.
AN - WOS:000472984900008
AU - Cawlfield, A.
AU - Genito, C. J.
AU - Beck, Z.
AU - Bergmann-Leitner, E. S.
AU - Bitzer, A. A.
AU - Soto, K.
AU - Zou, X. Y.
AU - Hadiwidjojo, S. H.
AU - Gerbasi, R. V.
AU - Mullins, A. B.
AU - Noe, A.
AU - Waters, N. C.
AU - Alving, C. R.
AU - Matyas, G. R.
AU - Dutta, S.
DA - JUN 27
DO - 10.1016/j.vaccine.2019.05.059
IS - 29
PY - 2019
SN - 0264-410X
1873-2518
SP - 3793-3803
ST - Safety, toxicity and immunogenicity of a malaria vaccine based on the
circumsporozoite protein (FMP013) with the adjuvant army liposome formulation
containing QS21 (ALFQ)
T2 - VACCINE
TI - Safety, toxicity and immunogenicity of a malaria vaccine based on the
circumsporozoite protein (FMP013) with the adjuvant army liposome formulation
containing QS21 (ALFQ)
VL - 37
ID - 6647
ER -
TY - JOUR
AB - Background: Despite the extensive use of silver ions or nanoparticles in
research related to preventing implant-associated infections (IAI), their use in
clinical practice has been debated. This is because the strong antibacterial
properties of silver are counterbalanced by adverse effects on host cells. One of
the reasons for this may be the lack of comprehensive in vitro models that are
capable of analyzing host-bacteria and host-host interactions. Methods and results:
In this study, we tested silver efficacy through multicellular in vitro models
involving macrophages (immune system), mesenchymal stem cells (MSCs, bone cells),
and S. aureus (pathogen). Our model showed to be capable of identifying each
element of culture as well as tracking the intracellular survival of bacteria.
Furthermore, the model enabled to find a therapeutic window for silver ions (AgNO3)
and silver nanoparticles (AgNPs) where the viability of host cells was not
compromised, and the antibacterial properties of silver were maintained. While
AgNO3 between 0.00017 and 0.017 µg/mL retained antibacterial properties, host cell
viability was not affected. The multicellular model, however, demonstrated that
those concentrations had no effect on the survival of S. aureus, inside or outside
host cells. Similarly, treatment with 20 nm AgNPs did not influence the phagocytic
and killing capacity of macrophages or prevent S. aureus from invading MSCs.
Moreover, exposure to 100 nm AgNPs elicited an inflammatory response by host cells
as detected by the increased production of TNF-α and IL-6. This was visible only
when macrophages and MSCs were cultured together. Conclusions: Multicellular in
vitro models such as the one used here that simulate complex in vivo scenarios can
be used to screen other therapeutic compounds or antibacterial biomaterials without
the need to use animals. Copyright © 2023 Cecotto, Stapels, van Kessel, Croes,
Lourens, Vogely, van der Wal, van Strijp, Weinans and Amin Yavari.
AU - Cecotto, L.
AU - Stapels, D. A. C.
AU - van Kessel, K. P. M.
AU - Croes, M.
AU - Lourens, Z.
AU - Vogely, H. C.
AU - van der Wal, B. C. H.
AU - van Strijp, J. A. G.
AU - Weinans, H.
AU - Amin Yavari, S.
C7 - 1186936
DB - Scopus
DO - 10.3389/fcimb.2023.1186936
KW - antimicrobial
biomaterial-related infection
co-culture
cytotoxicity
immune response
Animals
Bacteria
Metal Nanoparticles
Silver
biomaterial
interleukin 6
silver
silver nanoparticle
silver nitrate
antiinfective agent
metal nanoparticle
Article
bacterial survival
bacterium culture
bone marrow mesenchymal stem cell
cell culture
cell viability
coculture
colony forming unit
confocal microscopy
cytokine production
flow cytometry
host bacterium interaction
host cell
human
human cell
in vitro study
in vivo study
infection
inflammation
macrophage
microscopy
monocyte
nonhuman
animal
bacterium
M3 - Article
PY - 2023
ST - Evaluation of silver bio-functionality in a multicellular in vitro model:
towards reduced animal usage in implant-associated infection research
T2 - Frontiers in Cellular and Infection Microbiology
TI - Evaluation of silver bio-functionality in a multicellular in vitro model:
85162219526&doi=10.3389%2ffcimb.2023.1186936&partnerID=40&md5=ee0550bb5a85fefce11ed
3c4b6631ddf
VL - 13
ID - 5001
ER -
TY - JOUR
AB - BackgroundDespite the extensive use of silver ions or nanoparticles in
research related to preventing implant-associated infections (IAI), their use in
clinical practice has been debated. This is because the strong antibacterial
properties of silver are counterbalanced by adverse effects on host cells. One of
the reasons for this may be the lack of comprehensive in vitro models that are
capable of analyzing host-bacteria and host-host interactions. Methods and
resultsIn this study, we tested silver efficacy through multicellular in vitro
models involving macrophages (immune system), mesenchymal stem cells (MSCs, bone
cells), and S. aureus (pathogen). Our model showed to be capable of identifying
each element of culture as well as tracking the intracellular survival of bacteria.
Furthermore, the model enabled to find a therapeutic window for silver ions (AgNO3)
and silver nanoparticles (AgNPs) where the viability of host cells was not
compromised, and the antibacterial properties of silver were maintained. While
AgNO3 between 0.00017 and 0.017 mu g/mL retained antibacterial properties, host
cell viability was not affected. The multicellular model, however, demonstrated
that those concentrations had no effect on the survival of S. aureus, inside or
outside host cells. Similarly, treatment with 20 nm AgNPs did not influence the
phagocytic and killing capacity of macrophages or prevent S. aureus from invading
MSCs. Moreover, exposure to 100 nm AgNPs elicited an inflammatory response by host
cells as detected by the increased production of TNF-alpha and IL-6. This was
visible only when macrophages and MSCs were cultured together.
ConclusionsMulticellular in vitro models such as the one used here that simulate
complex in vivo scenarios can be used to screen other therapeutic compounds or
antibacterial biomaterials without the need to use animals.
AN - WOS:001009911000001
AU - Cecotto, L.
AU - Stapels, D. A. C.
AU - van Kessel, K. P. M.
AU - Croes, M.
AU - Lourens, Z.
AU - Vogely, H. C.
AU - van der Wal, B. C. H.
AU - van Strijp, J. A. G.
AU - Weinans, H.
AU - Yavari, S. A.
C7 - 1186936
DA - JUN 5
DO - 10.3389/fcimb.2023.1186936
PY - 2023
SN - 2235-2988
ST - Evaluation of silver bio-functionality in a multicellular in vitro model:
T2 - FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
TI - Evaluation of silver bio-functionality in a multicellular in vitro model:
VL - 13
ID - 6338
ER -
TY - JOUR
AB - Halloysite is a naturally-occurring nanomaterial occurring in the thousands
of tons and that serves as biomaterial, with applications in the areas of
biotechnology, pharmaceutical, and medical research. This study reports on the
anti-inflammatory, cytotoxic, and anti-oxidant activity of halloysite Jarrahdale
(collected at ~45km SE of Perth, Western Australia; JA), Dragon Mine (provided by
Natural Nano Inc., Rochester, New York; NA), and Kalgoorie Archean (collected at
Siberia, ~85km NW of Kalgoorlie, West Australia; PA). Prior to biological testing,
halloysites were characterized by 27Al and 29Si Nuclear Magnetic Resonance
Spectroscopy, the anti-inflammatory activity was determined by (a) the mouse ear
edema method, using 12-o-tetradecanoylphorbol-13-acetate (TPA) as anti-inflammatory
agent; and (b) the myeloperoxidase enzymatic activity method (MPO). Cell viability
was determined using the MTT method. Sample characterization by NMR method showed
similar symmetry and atomic environments, with no evidence of distortion(s) due to
shiftings in atomic ordering or electron density. The anti-inflammatory activity
followed the order: PA>JA>NA, and remained invariant with time. Prolonged anti-
inflammatory activity related inversely to surface area and lumen space. The low
extent of infiltration at shorter reaction times confirmed a limiting number of
active surface sites. EPR intensity signals followed the order: JA>NA>PA. The poor
stabilization of RO species in PA suspensions was explained by tube alignment
provoking occlusion, thus limiting transfer of H+ or e- from-and-to the surface,
and decreases in acidity associated to Aloct. Cell viability (%) varied from one
surface to the other, PA(92.3±6.0), JA(84.9±7.8), and NA(78.0±5.6), but related
directly to SBET values. © 2013 Elsevier B.V.
AU - Cervini-Silva, J.
AU - Nieto-Camacho, A.
AU - Palacios, E.
AU - Montoya, J. A.
AU - Gómez-Vidales, V.
AU - Ramírez-Apán, M. T.
DB - Scopus
DO - 10.1016/j.colsurfb.2013.06.056
KW - Surface morphology
Time-dependent infiltration
Aluminum Silicates
Animals
Cell Death
Cell Survival
Electrons
Ion Exchange
Macrophages, Peritoneal
Magnetic Resonance Spectroscopy
Mice
Peroxidase
Surface Plasmon Resonance
Surface Properties
Tetradecanoylphorbol Acetate
Biological materials
Cytotoxicity
Nuclear magnetic resonance spectroscopy
aluminum
dragon mine
hallosite
hydrogen
indometacin
jarrahdale
kalgoorie archean
myeloperoxidase
nanomaterial
silver
unclassified drug
aluminum silicate
antiinfective agent
clay
peroxidase
Active surface sites
Anti-inflammatories
Enzymatic activities
Perth , Western Australia
analytic method
animal cell
animal experiment
animal model
article
cell infiltration
cell viability
density
drug cytotoxicity
drug determination
ear edema
electron spin resonance
enzyme activity
mouse
nonhuman
priority journal
protein stability
proton transport
reaction time
surface property
suspension
animal
cell death
cell survival
cytology
drug effects
electron
ion exchange
metabolism
peritoneum macrophage
Kaolinite
M3 - Article
PY - 2013
SP - 651-655
ST - Anti-inflammatory and anti-bacterial activity, and CYTOTOXICITY of halloysite
surfaces
T2 - Colloids and Surfaces B: Biointerfaces
TI - Anti-inflammatory and anti-bacterial activity, and CYTOTOXICITY of halloysite
surfaces
84881245846&doi=10.1016%2fj.colsurfb.2013.06.056&partnerID=40&md5=2eb374e95d08c917a
280dc3d75618485
VL - 111
ID - 5631
ER -
TY - JOUR
AB - SUMMARY: The aim of this study is to determine the potential therapeutic
effects of CAPE in CP-induced nephrotoxicity in rats. Cisplatin (CP) is an
antineoplastic chemotherapeutic used for treatment of many cancer types but its
applications may induce nephrotoxicity. Caffeic acid phenethyl ester (CAPE) is an
active component of propolis and it has several important physiological activities.
Rats were divided into four groups: Control, CAPE (10 µmol/kg/i.p), CP (7
mg/kg/i.p), and CP+CAPE (7 mg/kg/i.p, CP and 10 µmol/kg/i.p, CAPE). After
administrations, animals were sacrificed, and kidney tissues were extracted.
Histopathological changes were evaluated and TNF-α and IL-6 immunostaining were
performed. Moreover, tissue SOD, CAT and MDA levels were measured by ELISA assay to
assessment of oxidative stress and lipid peroxidation. CP group showed
histopathological deterioration compared to the Control group and CAPE treatment
attenuated this damage. When compared with Control and CAPE group, an increase in
TNF-α and IL-6 immunoreactivities and tissue MDA levels were observed in the CP
group while a decrease in tissue SOD and CAT levels were detected. Furthermore, an
improvement was observed in the CP+CAPE compared to the CP group. We suggest that
CAPE can be used as a therapeutic agent to attenuate the toxic effects of
cisplatin, thanks to its antioxidant and anti-inflammatory properties.
RESUMEN: El objetivo de este estudio fue determinar los posibles efectos
terapéuticos de éster fenetílico del ácido cafeico (EFAC) en la nefrotoxicidad
inducida por cisplatino (CP) en ratas. El CP es un quimioterapéutico antineoplásico
utilizado para el tratamiento de muchos tipos de cáncer, sin embargo sus
aplicaciones pueden inducir nefrotoxicidad. El EFAC es un componente activo del
propóleo y tiene varias actividades fisiológicas importantes. Para el estudio las
ratas se dividieron en cuatro grupos: Control, EFAC (10 µmol / kg / ip), CP (7 mg /
kg / ip) y CP + EFAC (7 mg / kg / ip, CP y 10 µmol / kg / ip, EFAC). Después de las
administraciones, se sacrificaron los animales y se extrajeron los tejidos renales.
Se evaluaron los cambios histopatológicos y se realizó inmunotinción de TNF-α e IL-
6. Además, los niveles tisulares de SOD, CAT y MDA se midieron mediante un ensayo
ELISA para evaluar el estrés oxidativo y la peroxidación lipídica. El grupo CP
mostró deterioro histopatológico en comparación con el grupo Control y el
tratamiento con EFAC atenuó este daño. En comparación con el grupo de control y
EFAC, se observó un aumento en las inmunorreactividades de TNF-α e IL-6 y los
niveles de MDA en el tejido en el grupo de CP, mientras que se detectó una
disminución en los niveles de SOD y CAT en los tejidos. Además, se observó una
mejora en el CP + EFAC en comparación con el grupo CP. Sugerimos que EFAC puede
utilizarse como agente terapéutico para atenuar los efectos tóxicos del cisplatino,
gracias a sus propiedades antioxidantes y antiinflamatorias.
AD - Ceylan, Tayfun
Cappadocia University. Cappadocia Vocational School. Department of Medical Services
and Technique. Pathology Laboratory Techniques Pr. Nevsehir. TR
Kaymak, Emin
Yozgat Bozok University. Faculty of Medicine. Histology-Embryology Department.
Yozgat. TR
Akin, Ali Tugrul
Erciyes University. Science Faculty. Biology Department. Kayseri. TR
Yakan, Birkan
Erciyes University. Faculty of Medicine. Histology-Embryology Department. Kayseri.
TR
AU - Ceylan, Tayfun
AU - Kaymak, Emin
AU - Akin, Ali Tugrul
AU - Yakan, Birkan
C1 - 20220812
DA - 2021/04
DB - LILACS
DO - 10.4067/S0717-95022021000200612
IS - 2
KW - Caffeic acid phenethyl ester
Cisplatin
Cisplatino
Estrés oxidativo
Inflamación
Inflammation
Oxidative stress
Éster fenetílico del ácido cafeico
LA - en
PY - 2021
SN - 0717-9367
SP - 612-618
ST - The ameliorative effects of caffeic acid phenethyl ester in cisplatin-induced
nephrotoxicity: assessment of the oxidative stress an inflammation
TI - The ameliorative effects of caffeic acid phenethyl ester in cisplatin-induced
nephrotoxicity: assessment of the oxidative stress an inflammation
TT - Los efectos de mejora del éster fenetílico del ácido cafeico en la
nefrotoxicidad inducida por cisplatino: evaluación del estrés oxidativo y la
inflamación
VL - 39
ID - 4919
ER -
TY - JOUR
AB - The increased use of nano-sized metallic materials is likely to result in the
release of these particles into the environment. It is, however, unclear if these
materials are harmful to aquatic animals. Furthermore, because the dissolution of
such nanomaterials will occur, it is probable that some of the adverse effects
resulting will result from the dissolved metal species. In this study, therefore,
we investigated the health and environmental impact of silver nanoparticles (Ag-
NPs) on Japanese Medaka by studying changes in the expression of stress-related
genes using real time RT-PCR analysis and compared these results with those of
Medaka exposed to soluble silver ions. The stress-related genes selected here were
metallothionein, HSP 70, GST, p53, CYP 1A and the transferrin gene. The expression
levels of each gene were determined using two different Ag-NPs dosages and were
quantified by measuring the mRNA concentrations in liver extracts with the Taqman-
based Real-Time PCR method. The results suggest that these two silver forms have
distinguishable toxic fingerprints between them. While the Ag-NPs led to cellular
and DNA damage, as well as carcinogenic and oxidative stresses, genes related with
metal detoxification/metabolism regulation and radical scavenging action were also
induced. In contrast, the ionic silver led to an induction of inflammatory response
and metallic detoxification processes in the liver of the exposed fish, but
resulted in a lower overall stress response when compared with the Ag-NPs. © 2009
Elsevier B.V. All rights reserved.
AU - Chae, Y. J.
AU - Pham, C. H.
AU - Lee, J.
AU - Bae, E.
AU - Yi, J.
AU - Gu, M. B.
DB - Scopus
DO - 10.1016/j.aquatox.2009.07.019
IS - 4
KW - Japanese Medaka
Nanotoxicity
Real-time PCR
Silver nanoparticle
Animals
DNA Damage
Liver
Metal Nanoparticles
Oryzias
Oxidative Stress
RNA, Messenger
Silver
Water Pollutants, Chemical
Animalia
Oryzias latipes
Oryziinae
cytochrome P450 1A
messenger RNA
metallothionein
nanoparticle
protein p53
silver
silver nanoparticle
transferrin
unclassified drug
concentration (composition)
detoxification
dissolution
dose-response relationship
environmental impact
fish
mitochondrial DNA
toxicity test
animal experiment
animal tissue
article
carcinogenesis
cell damage
controlled study
DNA damage
ecotoxicology
environmental impact assessment
inflammation
liver parenchyma
metal metabolism
nanotoxicology
nonhuman
nucleotide sequence
oxidative stress
priority journal
stress
water contamination
M3 - Article
PY - 2009
SP - 320-327
ST - Evaluation of the toxic impact of silver nanoparticles on Japanese medaka
(Oryzias latipes)
T2 - Aquatic Toxicology
TI - Evaluation of the toxic impact of silver nanoparticles on Japanese medaka
(Oryzias latipes)
69949141979&doi=10.1016%2fj.aquatox.2009.07.019&partnerID=40&md5=bd40c9314b5b3ce4e2
abfc04c1a21ced
VL - 94
ID - 5766
ER -
TY - JOUR
AB - In the current study, silver nanoparticles (AgNPs) were biosynthesized using
galbanic acid (GA), a sesquiterpene coumarin. The formation of GA-AgNPs was
characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform
infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), Energy
Dispersive X-ray (EDX), and X-ray diffraction (XRD). The biosynthesized GA-AgNPs
were spherical in shape with an average particle size of 142.33 +/- 32.6 nm. The
results from the antibacterial assays sug-gested that biosynthesized GA-AgNPs were
more potent against multi-drug resistant (MDR) and non -MDR pathogenic bacteria
than the crude GA alone. The nanoparticles also showed potent antiproliferative
potential against H1229 and MCF-7 cancer cells with IC50 values of 25 mu g/mL and
50 mu g/mL by the MTT assay, respectively. These NPs showed good antioxidant (30 %
at 100 mu g/mL), anti-inflammatory (99.5 % at 500 mu g/mL), and anti-coagulant
properties without significant hemolysis on red blood cells (RBCs). These results
confirm the benefits of using the green, simple, and cost-effective manner for the
synthesis of AgNPs with excellent biological properties and hemocompatibility.(c)
2022 The Society of Powder Technology Japan. Published by Elsevier B.V. and The
Society of Powder Technology Japan. All rights reserved.
AN - WOS:000911829100001
AU - Chahardoli, A.
AU - Mavaei, M.
AU - Shokoohinia, Y.
AU - Fattahi, A.
C6 - DEC 2022
C7 - 103928
DA - JAN
DO - 10.1016/j.apt.2022.103928
IS - 1
PY - 2023
SN - 0921-8831
1568-5527
ST - Galbanic acid, a sesquiterpene coumarin as a novel candidate for the
biosynthesis of silver nanoparticles: In vitro hemocompatibility,
antiproliferative, antibacterial, antioxidant, and anti-inflammatory properties
T2 - ADVANCED POWDER TECHNOLOGY
TI - Galbanic acid, a sesquiterpene coumarin as a novel candidate for the
biosynthesis of silver nanoparticles: In vitro hemocompatibility,
antiproliferative, antibacterial, antioxidant, and anti-inflammatory properties
VL - 34
ID - 6031
ER -
TY - JOUR
AB - The effects of ingestion of engineered nanoparticles (NPs), especially via
drinking water, are unknown. Using NPs spiked into synthetic water and cell culture
media, we investigated cell death, oxidative stress, and inflammatory effects of
silver (Ag), titanium dioxide (TiO2), and zinc oxide (ZnO) NPs on human intestinal
Caco-2 and SW480 cells. ZnO NPs were cytotoxic to both cell lines, while Ag and
TiO2 NPs were toxic only at 100 mg/L to Caco-2 and SW480, respectively. ZnO NPs led
to significant cell death in synthetic freshwaters with 1 % phosphate-buffered
saline in both cell lines, while Ag and TiO2 NPs in buffered water led to cell
death in SW480 cells. NP exposures did not yield significant increased reactive
oxygen species generation but all NP exposures led to increased IL-8 cytokine
generation in both cell lines. These results indicate cell stress and cell death
from NP exposures, with a varied response based on NP composition.
AN - WOS:000316228400003
AU - Chalew, T. E. A.
AU - Schwab, K. J.
DA - APR
DO - 10.1007/s10565-013-9241-6
IS - 2
PY - 2013
SN - 0742-2091
1573-6822
SP - 101-116
ST - Toxicity of commercially available engineered nanoparticles to Caco-2 and
T2 - CELL BIOLOGY AND TOXICOLOGY
TI - Toxicity of commercially available engineered nanoparticles to Caco-2 and
VL - 29
ID - 6301
ER -
TY - JOUR
AB - Since the discovery of mutant Janus Kinase 2 (JAK2), JAK2V617F, in a major
proportion of myeloproliferative neoplasm (MPN) patients, there has been a flurry
of activity in the development of JAK2 inhibitors. Pan-JAK, predominantly JAK2 and
off-target JAK2 inhibitors have been developed in the short span of the past 5
years. These compounds have since been tested to varying success in both in vitro
and in vivo settings with several proceeding on to advanced clinical trials.
Although it was hoped that these inhibitors would be the silver bullet in the
manner than imatinib was to chronic myeloid leukemia, it is becoming apparent that
this is not the case for various reasons, chief of which is that a significant
reduction of the underlying pathogenic clone is not achieved. In fact, the very
notion that the target of JAK2 inhibitors (be it pan-JAK or JAK2 specific) is the
mutant JAK2V617F is being challenged with findings from several clinical trials
showing a poor correlation between the reduction in JAK2V617F mutant allele burden
and clinical response. In view of this, it is not surprising that several groups
are now investigating combinations of JAK2 inhibitors and other agents in MPN.
Although much knowledge has been added in this short span of time, it is apparent
that our understanding of the role of JAK2 inhibitors in the treatment scheme of
MPN is only beginning. © 2011, SAGE Publications. All rights reserved.
AU - Chan, D.
AU - Koren Michowitz, M.
DB - Scopus
DO - 10.1177/2040620711401646
IS - 2
KW - JAK2 inhibitors
JAK2V617F
myeloproliferative neoplasm
1 cyclopropyl 3 [3 (5 morpholinomethyl 1h benzimidazol 2 yl) 1h pyrazol 4 yl]urea
5 chloro n2 [1 (5 fluoro 2 pyrimidinyl)ethyl] n4 (5 methyl 1h pyrazol 3 yl) 2,4
pyrimidinediamine
anagrelide
antithrombocytic agent
bms 911543
C reactive protein
erlotinib
gandotinib
givinostat
hydroxyurea
interleukin 1 receptor blocking agent
interleukin 6
Janus kinase 2 inhibitor
Janus kinase inhibitor
lenalidomide
lestaurtinib
ls 104
momelotinib
n tert butyl 3 [5 methyl 2 [4 [2 (1 pyrrolidinyl)ethoxy]phenylamino] 4
pyrimidinylamino]benzenesulfonamide
ns 018
ns 108
pacritinib
panobinostat
peginterferon
pomalidomide
ruxolitinib
tofacitinib
unclassified drug
wp 1066
xl 019
abdominal pain
acute leukemia
advanced cancer
allele
anemia
blast transformation
bone marrow suppression
cancer chemotherapy
chronic myelomonocytic leukemia
cytoreductive surgery
diarrhea
drug activity
drug cytotoxicity
drug dose reduction
drug efficacy
drug response
drug tolerability
fatigue
follow up
gene mutation
hematologic disease
Hodgkin disease
human
leukocytosis
lung metastasis
lung non small cell cancer
lymphoma
myelofibrosis
myeloid leukemia
myeloid metaplasia
myelomonocytic leukemia
nausea
neurotoxicity
neutropenia
nonhuman
pathogenesis
phase 2 clinical trial (topic)
phlebotomy
polycythemia vera
postoperative complication
priority journal
protein blood level
rash
review
splenomegaly
symptom assessment
thrombocythemia
thrombocytopenia
thrombocytosis
M3 - Review
PY - 2011
SP - 61-71
ST - Update on JAK2 inhibitors in myeloproliferative neoplasm
T2 - Therapeutic Advances in Hematology
TI - Update on JAK2 inhibitors in myeloproliferative neoplasm
84993810398&doi=10.1177%2f2040620711401646&partnerID=40&md5=8bc6533748879e0b12a1660
13d43339e
VL - 2
ID - 5676
ER -
TY - JOUR
AB - In this report, a novel nanocomposite based on type I collagen (Col) was
prepared, which contained varying amounts (15.1, 30.2, and 75.5 ppm) of silver
nanoparticles (AgNPs). The surface morphology and chemical composition pure Col and
Col-AgNP nanocomposites (Col-Ag) was characterized by UV–Vis spectroscopy (UV–Vis),
atomic force microscope (AFM) and Fourier transform IR spectrometer (FTIR). The
biocompatibility effect and biological activity of Col-Ag culturing with
mesenchymal stem cells (MSCs), as well as guiding for angiogenesis differentiation,
were evaluated via in vitro assay. The Col-Ag in 30.2 ppm demonstrated better
biological properties and compatibility culturing with MSCs. The biological
properties could be associated with cell adhesion, proliferation, migration and
differentiation. Afterwards, the induced angiogenesis and differentiation of MSCs
by the expression of von Willebrand Factor (vWF) and CD31 were also investigated.
Furthermore, both anti-inflammatory and endothelialization ability were also
investigated in vivo assay. It was observed that Col-Ag nanocomposites not only
inhibited CD86 expression, but also facilitated endothelialization capacity, the
expression of CD31 when implanting Col-Ag into rats subcutaneously after 4 weeks.
This current research indicates that Col-Ag nanocomposites has potential of being
employed as a surface modification approach, and is better in clinical treatments
with MSCs for vascular regeneration applications. © 2021
AU - Chang, K. B.
AU - Shen, C. C.
AU - Hsu, S. H.
AU - Tang, C. M.
AU - Yang, Y. C.
AU - Liu, S. Y.
AU - Ku, T. R.
AU - Kung, M. L.
AU - Hsieh, H. H.
AU - Hung, H. S.
C7 - 126814
DB - Scopus
DO - 10.1016/j.colsurfa.2021.126814
Differentiation
Mesenchymal stem cells
Type I collagen
Vascular regeneration
Atomic force microscopy
Cell culture
Clinical research
Collagen
Differentiation (calculus)
Morphology
Nanocomposites
Spectrometers
Stem cells
Surface morphology
actin
collagen type 1
Hermes antigen
integrin
nanocomposite
platelet endothelial cell adhesion molecule 1
silver nanoparticle
stromal cell derived factor 1alpha
vasculotropin
vasculotropin receptor 2
von Willebrand factor
Ag$++$
Angiogenesis
Biological properties
Endothelialization
Functionalized
Mesenchymal stem cell
Type-I collagen
angiogenesis
animal cell
animal experiment
apoptosis
Article
biocompatibility
cell adhesion
cell interaction
cell migration
cell proliferation
cell survival rate
cell viability
chemical structure
collagen fiber
comparative study
controlled study
cytoskeleton
female
flow cytometry
fluorescence activated cell sorting
Fourier transform spectroscopy
human
human cell
immunophenotyping
in vitro study
in vivo study
nonhuman
protein expression
rat
stem cell culture
surface property
tissue regeneration
TUNEL assay
zeta potential
M3 - Article
PY - 2021
ST - Functionalized collagen-silver nanocomposites for evaluation of the
biocompatibility and vascular differentiation capacity of mesenchymal stem cells
T2 - Colloids and Surfaces A: Physicochemical and Engineering Aspects
TI - Functionalized collagen-silver nanocomposites for evaluation of the
biocompatibility and vascular differentiation capacity of mesenchymal stem cells
85106900438&doi=10.1016%2fj.colsurfa.2021.126814&partnerID=40&md5=66dfcd08c6e7afb9c
2af601d9c8b37eb
VL - 624
ID - 5222
ER -
TY - JOUR
AB - With the development of nanotechnology, metal-containing nanoparticles are
used widely in the diagnosis, monitoring and treatment of central nervous system
(CNS) diseases. The neurotoxicity of these nanoparticles has drawn attention. Glial
cells (particularly microglial cells and astrocytes) have important functions in
the CNS. Neural disorders are related to functional/histologic damage to glial
cells. Dysfunctions of microglial cells or astrocytes injure the brain, and cause
the neurodegeneration seen in Alzheimer's disease and Parkinson's disease. We have
summarized the route of access of metal-containing nanoparticles to the CNS, as
well as their neurotoxicity and potential molecular mechanisms involved in glial
cells. Metal-containing nanoparticles cross or bypass the blood-brain barrier,
access the CNS and cause neurotoxicity. The potential mechanisms are related to
inflammation, oxidative stress, DNA and/or mitochondrial damage and cell death, all
of which are mediated by microglial cell activation, inflammatory factor release,
generation of reactive oxygen species, apoptosis and/or autophagy in glial cells.
Moreover, these processes increase the burden of the CNS and even accelerate the
occurrence or development of neurodegenerative diseases. Some important signaling
pathways involved in the mechanism of neurotoxicity in glial cells caused by
nanoparticles are also discussed.
AN - WOS:000550438600001
AU - Chang, X. R.
AU - Li, J. Y.
AU - Niu, S. Y.
AU - Xue, Y. Y.
AU - Tang, M.
C6 - JUL 2020
DA - JAN
DO - 10.1002/jat.4037
IS - 1
PY - 2021
SN - 0260-437X
1099-1263
SP - 65-81
ST - Neurotoxicity of metal-containing nanoparticles and implications in glial
cells
TI - Neurotoxicity of metal-containing nanoparticles and implications in glial
cells
VL - 41
ID - 6509
ER -
TY - JOUR
AB - The aim of this work is to understand the potential health effects of metal
nanoparticles by exposing human leukemic cell lines (jurkat, K562 and KG1A cells)
to nanosize phosphonomethyl iminodiacetic acid coated cobalt oxide (PMIDA-CoO) NPs.
The synthesized PMIDA-CoO NPs were characterized by XRD, dynamic light scattering,
transmission electron microscopy and scanning electron microscopy. Our results
showed that exposure of leukemic cell lines to PMIDA-CoO NPs caused reactive oxygen
species (ROS) generation by increasing the concentration of free Co++ ions in
cancer microenvironment. But at physiological pH, PMIDA-CoO liberates little amount
of Co++ ions into media and exerts lower toxicity to normal cells up to a certain
dose. PMIDA-CoO NPs caused DNA damage in leukemic cell lines, which was reflected
by an increase in apoptosis of jurkat, KG-1A and K562 cells. PMIDA-CoO NPs induced
apoptosis by increasing pro-inflammatory cytokines, primarily TNF-alpha. The in
vivo study shows that PMIDA-CoO NPs were efficiently killed DLA cells. These
findings have important implications for understanding the potential anticancer
property induced by surface-modified cobalt oxide nanoparticles.
AN - WOS:000347407700011
AU - Chattopadhyay, S.
AU - Dash, S. K.
AU - Tripathy, S.
AU - Pramanik, P.
AU - Roy, S.
DA - JAN
DO - 10.1007/s00775-014-1221-7
IS - 1
PY - 2015
SN - 0949-8257
1432-1327
SP - 123-141
ST - Phosphonomethyl iminodiacetic acid-conjugated cobalt oxide nanoparticles
liberate Co++ ion-induced stress associated activation of TNF-alpha/p38
MAPK/caspase 8-caspase 3 signaling in human leukemia cells
T2 - JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
TI - Phosphonomethyl iminodiacetic acid-conjugated cobalt oxide nanoparticles
liberate Co++ ion-induced stress associated activation of TNF-alpha/p38
MAPK/caspase 8-caspase 3 signaling in human leukemia cells
VL - 20
ID - 6371
ER -
TY - JOUR
AB - Intermittent exposure to air is used as a protective strategy against
hyperbaric O-2 (HBO2) toxicity. Little is known about optimal intermittent exposure
schedules and the mechanism of protection. In this study, we examined the role of
antioxidant enzymes, and inflammatory cytokines in the mechanism of HBO2 tolerance
by intermittent air breaks. One group of rats was exposed continuously to 282 kPa
O-2 until death. Other groups were exposed to 30, 60, and 120 min intervals of HBO2
with different numbers of intermittent 30 min air breaks (1-12 breaks). After the
final break, animals were exposed to HBO2 until death. In a separate experiment,
animals were sacrificed before terminal exposure and lung tissues were collected
for analysis of gene expression. Two intermittent schedules with 6 h cumulative O-2
time (30/30 and 60/30 min schedules) were compared with continuous exposure to HBO2
for 6 h and with intermittent exposure of 8 h (120/30 min schedule) duration.
Continuous exposure resulted in activation of inflammatory cytokine TNF-alpha and
IL-1 beta mRNA expression, an increase in lung protein nitration and activation of
inducible NOS (iNOS) mRNA. Inflammatory response was not observed at intermittent
exposures of the same cumulative O-2 time duration (30/30 and 60/30 min schedule).
Expression of heme oxygenase-1 (HO-1) mRNA was significantly increased in all
exposure groups while manganese superoxide dismutase (MnSOD) mRNA expression was
increased only in continuous and 120/30 exposure groups. Results show that
intermittent exposure to air protects against pulmonary HBO2 toxicity by inhibiting
inflammation. The mechanism of inhibition may involve the antiinflammatory and
antioxidative effect of HO-1 but some other mechanisms may also be involved in
protection by intermittent air breaks.
AN - WOS:000252540300004
AU - Chavko, M.
AU - Mahon, R. T.
AU - McCarron, R. M.
DA - MAR
DO - 10.1007/s00421-007-0611-8
IS - 5
PY - 2008
SN - 1439-6319
1439-6327
SP - 525-532
ST - Mechanisms of protection against pulmonary hyperbaric O-2 toxicity by
intermittent air breaks
T2 - EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
TI - Mechanisms of protection against pulmonary hyperbaric O-2 toxicity by
intermittent air breaks
VL - 102
ID - 6405
ER -
TY - JOUR
AB - Aloe vera-based hydrogels have emerged as promising platforms for the
delivery of therapeutic agents in wound dressings due to their biocompatibility and
unique wound-healing properties. The present study provides a comprehensive
overview of recent advances in the application of Aloe vera-based hydrogels for
wound healing. The synthesis methods, structural characteristics, and properties of
Aloe vera-based hydrogels are discussed. Mechanisms of therapeutic agents released
from Aloe vera-based hydrogels, including diffusion, swelling, and degradation, are
also analyzed. In addition, the therapeutic effects of Aloe vera-based hydrogels on
wound healing, as well as the reduction of inflammation, antimicrobial activity,
and tissue regeneration, are highlighted. The incorporation of various therapeutic
agents, such as antimicrobial and anti-inflammatory ones, into Aloe vera-based
hydrogels is reviewed in detail. Furthermore, challenges and future prospects of
Aloe vera-based hydrogels for wound dressing applications are considered. This
review provides valuable information on the current status of Aloe vera-based
hydrogels for the delivery of therapeutic agents in wound dressings and highlights
their potential to improve wound healing outcomes.
AN - WOS:001035917900001
AU - Chelu, M.
AU - Musuc, A. M.
AU - Popa, M.
AU - Moreno, J. C.
C7 - 539
DA - JUL
DO - 10.3390/gels9070539
IS - 7
PY - 2023
SN - 2310-2861
ST - Aloe vera-Based Hydrogels for Wound Healing: Properties and Therapeutic
Effects
T2 - GELS
TI - Aloe vera-Based Hydrogels for Wound Healing: Properties and Therapeutic
Effects
VL - 9
ID - 6634
ER -
TY - JOUR
AB - In this study, we prepared core-sheath nanofiber membranes (CSNFMs) with
silver nanopar-ticles (Ag NPs) embedding in the polylactic acid (PLA) nanofiber
sheath and hyaluronic acid (HA) in the nanofiber core. The PLA/Ag NPs sheath
provides mechanical support as well as anti-bacterial and anti-inflammatory
properties. The controlled release of HA from the core could exert anti-adhesion
effects to promote tendon sliding while reducing fibroblast attachment. From the
microfibrous structural nature of CSNFMs, they function as barrier membranes to
reduce fibroblast penetration without hampering nutrient transports to prevent
post-operative peritendinous adhesion. As the anti-adhesion efficacy will depend on
release rate of HA from the core as well as Ag NP from the sheath, we fabricated
CSNFMs of comparable fiber diameter, but with thick (Tk) or thin (Tn) sheath.
Similar CSNFMs with thick (Tk+) and thin (Tn+) sheath but with embedded Ag NPs in
the sheath were also prepared. The physico-chemical properties of the barrier
membranes were characterized in details, together with their biological response
including cell penetration, cell attachment and proliferation, and cytotoxicity.
Peritendinous anti-adhesion models in rabbits were used to test the efficacy of
CSNFMs as anti-adhesion barriers, from gross observation, histology, and
biomechanical tests. Overall, the CSNFM with thin-sheath and Ag NPs (Tn+) shows
antibacterial activity with low cytotoxicity, prevents fibroblast penetration, and
exerts the highest efficacy in reducing fibroblast attachment in vitro. From in
vivo studies, the Tn+ membrane also shows significant improvement in preventing
peritendinous adhesions as well as anti-inflammatory efficacy, compared with Tk and
Tn CSNFMs and a commercial adhesion barrier film (SurgiWrap®) made from PLA. © 2021
by the authors. Licensee MDPI, Basel, Switzerland.
AU - Chen, C. H.
AU - Cheng, Y. H.
AU - Chen, S. H.
AU - Chuang, A. D. C.
AU - Chen, J. P.
C7 - 8781
DB - Scopus
DO - 10.3390/ijms22168781
IS - 16
KW - Anti-adhesion
Core-sheath nanofibers
Electrospinning
Hyaluronic acid
Polylactic acid
3T3 Cells
Animals
Cell Proliferation
Cell Survival
Gram-Negative Bacteria
Gram-Positive Bacteria
Hyaluronic Acid
Metal Nanoparticles
Mice
Nanofibers
Photoelectron Spectroscopy
Polyesters
Rabbits
Silver
Tendon Injuries
Tissue Adhesions
hyaluronic acid
nanofiber
polylactic acid
silver nanoparticle
antiinfective agent
metal nanoparticle
polyester
polylactide
silver
animal cell
animal experiment
animal model
animal tissue
Article
cell adhesion
cell culture
cell migration
cell proliferation
confocal laser scanning microscopy
controlled study
cytotoxicity
DNA determination
electrospinning
fibroblast
field emission scanning electron microscopy
flexor tendon
in vitro study
Leporidae
nonhuman
pore size
tendon
tendon rupture
tissue adhesion
X ray diffraction
3T3 cell line
animal
cell survival
chemistry
disease model
drug effect
mouse
tendon injury
M3 - Article
PY - 2021
ST - Functional hyaluronic acid-polylactic acid/silver nanoparticles core-sheath
nanofiber membranes for prevention of post-operative tendon adhesion
T2 - International Journal of Molecular Sciences
TI - Functional hyaluronic acid-polylactic acid/silver nanoparticles core-sheath
nanofiber membranes for prevention of post-operative tendon adhesion
85112545872&doi=10.3390%2fijms22168781&partnerID=40&md5=3b49a656939b0ef5af691925d2a
d650a
VL - 22
ID - 5215
ER -
TY - JOUR
AB - In this study, we prepared core-sheath nanofiber membranes (CSNFMs) with
silver nanoparticles (Ag NPs) embedding in the polylactic acid (PLA) nanofiber
sheath and hyaluronic acid (HA) in the nanofiber core. The PLA/Ag NPs sheath
provides mechanical support as well as anti-bacterial and anti-inflammatory
properties. The controlled release of HA from the core could exert anti-adhesion
effects to promote tendon sliding while reducing fibroblast attachment. From the
microfibrous structural nature of CSNFMs, they function as barrier membranes to
reduce fibroblast penetration without hampering nutrient transports to prevent
post-operative peritendinous adhesion. As the anti-adhesion efficacy will depend on
release rate of HA from the core as well as Ag NP from the sheath, we fabricated
CSNFMs of comparable fiber diameter, but with thick (Tk) or thin (Tn) sheath.
Similar CSNFMs with thick (Tk(+)) and thin (Tn(+)) sheath but with embedded Ag NPs
in the sheath were also prepared. The physico-chemical properties of the barrier
membranes were characterized in details, together with their biological response
including cell penetration, cell attachment and proliferation, and cytotoxicity.
Peritendinous anti-adhesion models in rabbits were used to test the efficacy of
CSNFMs as anti-adhesion barriers, from gross observation, histology, and
biomechanical tests. Overall, the CSNFM with thin-sheath and Ag NPs (Tn(+)) shows
antibacterial activity with low cytotoxicity, prevents fibroblast penetration, and
exerts the highest efficacy in reducing fibroblast attachment in vitro. From in
vivo studies, the Tn(+) membrane also shows significant improvement in preventing
peritendinous adhesions as well as anti-inflammatory efficacy, compared with Tk and
Tn CSNFMs and a commercial adhesion barrier film (SurgiWrap(R)) made from PLA.
AN - WOS:000689186100001
AU - Chen, C. H.
AU - Cheng, Y. H.
AU - Chen, S. H.
AU - Chuang, A. D. C.
AU - Chen, P.
C7 - 8781
DA - AUG
DO - 10.3390/ijms22168781
IS - 16
PY - 2021
SN - 1422-0067
ST - Functional Hyaluronic Acid-Polylactic Acid/Silver Nanoparticles Core-Sheath
Nanofiber Membranes for Prevention of Post-Operative Tendon Adhesion
T2 - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
TI - Functional Hyaluronic Acid-Polylactic Acid/Silver Nanoparticles Core-Sheath
Nanofiber Membranes for Prevention of Post-Operative Tendon Adhesion
VL - 22
ID - 5965
ER -
TY - JOUR
AB - Poor wound healing after diabetes or extensive burn remains a challenging
problem. Recently, we presented a physical approach to fabricate ultrasmall silver
particles from Ångstrom scale to nanoscale and determined the antitumor efficacy of
Ångstrom-scale silver particles (AgÅPs) in the smallest size range. Here we used
the medium-sized AgÅPs (65.9 ± 31.6 Å) to prepare carbomer gel incorporated with
these larger AgÅPs (L-AgÅPs-gel) and demonstrated the potent broad-spectrum
antibacterial activity of L-AgÅPs-gel without obvious toxicity on wound healing-
related cells. Induction of reactive oxygen species contributed to L-AgÅPs-gel-
induced bacterial death. Topical application of L-AgÅPs-gel to mouse skin triggered
much stronger effects than the commercial silver nanoparticles (AgNPs)-gel to
prevent bacterial colonization, reduce inflammation, and accelerate diabetic and
burn wound healing. L-AgÅPs were distributed locally in skin without inducing
systemic toxicities. This study suggests that L-AgÅPs-gel represents an effective
and safe antibacterial and anti-inflammatory material for wound therapy. Copyright
© 2020 The Authors, some rights reserved; exclusive licensee American Association
for the Advancement of Science. No claim to original U.S. Government Works.
Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-
NC).
AU - Chen, C. Y.
AU - Yin, H.
AU - Chen, X.
AU - Chen, T. H.
AU - Liu, H. M.
AU - Rao, S. S.
AU - Tan, Y. J.
AU - Qian, Y. X.
AU - Liu, Y. W.
AU - Hu, X. K.
AU - Luo, M. J.
AU - Wang, Z. X.
AU - Liu, Z. Z.
AU - Cao, J.
AU - He, Z. H.
AU - Wu, B.
AU - Yue, T.
AU - Wang, Y. Y.
AU - Xia, K.
AU - Luo, Z. W.
AU - Wang, Y.
AU - Situ, W. Y.
AU - Liu, W. E.
AU - Tang, S. Y.
AU - Xie, H.
C7 - aba0942
DB - Scopus
DO - 10.1126/sciadv.aba0942
IS - 43
KW - Acrylic Resins
Animals
Burns
Inflammation
Metal Nanoparticles
Mice
Silver
Wound Healing
Pathology
Tissue regeneration
Toxicity
acrylic acid resin
antiinfective agent
carbomer
metal nanoparticle
silver
Anti-inflammatories
Anti-tumor efficacy
Bacterial colonization
Physical approaches
Systemic toxicities
Topical application
animal
burn
inflammation
mouse
wound healing
M3 - Article
PY - 2020
ST - Ångstrom-scale silver particle-embedded carbomer gel promotes wound healing
by inhibiting bacterial colonization and inflammation
T2 - Science Advances
TI - Ångstrom-scale silver particle-embedded carbomer gel promotes wound healing
85094684662&doi=10.1126%2fsciadv.aba0942&partnerID=40&md5=1d2b7f52834fe745a3a3a15af
f65d7c2
VL - 6
ID - 5264
ER -
TY - JOUR
AB - Poor wound healing after diabetes or extensive burn remains a challenging
problem. Recently, we presented a physical approach to fabricate ultrasmall silver
particles from angstrom ngstrom scale to nanoscale and determined the antitumor
efficacy of angstrom ngstrom-scale silver particles (Ag angstrom Ps) in the
smallest size range. Here we used the medium-sized Ag angstrom Ps (65.9 +/- 31.6
angstrom) to prepare carbomer gel incorporated with these larger Ag angstrom Ps (L-
Ag angstrom Ps-gel) and demonstrated the potent broad-spectrum antibacterial
activity of L-Ag angstrom Ps-gel without obvious toxicity on wound healing-related
cells. Induction of reactive oxygen species contributed to L-Ag angstrom Ps-gel-
induced bacterial death. Topical application of L-Ag angstrom Ps-gel to mouse skin
triggered much stronger effects than the commercial silver nanoparticles (AgNPs)-
gel to prevent bacterial colonization, reduce inflammation, and accelerate diabetic
and burn wound healing. L-Ag angstrom Ps were distributed locally in skin without
inducing systemic toxicities. This study suggests that L-Ag angstrom Ps-gel
represents an effective and safe antibacterial and anti-inflammatory material for
wound therapy.
AN - WOS:000582114600005
AU - Chen, C. Y.
AU - Yin, H.
AU - Chen, X.
AU - Chen, T. H.
AU - Liu, H. M.
AU - Rao, S. S.
AU - Tan, Y. J.
AU - Qian, Y. X.
AU - Liu, Y. W.
AU - Hu, X. K.
AU - Luo, M. J.
AU - Wang, Z. X.
AU - Liu, Z. Z.
AU - Cao, J.
AU - He, Z. H.
AU - Wu, B.
AU - Yue, T.
AU - Wang, Y. Y.
AU - Xia, K.
AU - Luo, Z. W.
AU - Wang, Y.
AU - Situ, W. Y.
AU - Liu, W. E.
AU - Tang, S. Y.
AU - Xie, H.
C7 - eaba0942
DA - OCT
DO - 10.1126/sciadv.aba0942
IS - 43
PY - 2020
SN - 2375-2548
ST - Angstrom-scale silver particle-embedded carbomer gel promotes wound healing
T2 - SCIENCE ADVANCES
TI - Angstrom-scale silver particle-embedded carbomer gel promotes wound healing
VL - 6
ID - 6285
ER -
TY - JOUR
AB - PURPOSE. This exploratory study aimed to investigate the morphological and
pathological alterations of the meibomian gland (MG) with the Staphylococcus aureus
crude extracts (SACEs) treatment. METHODS. Mouse MG explants were cultured and
differentiated with or without SACEs for 48 hours. Explant's viability and cell
death were determined by thiazolyl blue tetrazolium bromide (MTT) assay and TUNEL
assay. MG morphology was observed by Hematoxylin and Eosin staining. Lipid droplet
production was detected by Nile Red staining and LipidTox immunostaining. The pro-
inflammatory cytokines were detected by ELISA. The relative gene and protein
expression in MG explants was determined via quantitative RT-PCR, immunostaining,
and immunoblotting. The components of the SACEs were analyzed by immunoblotting and
silver staining. RESULTS. Our findings demonstrated that the SACEs treatment
induced overexpression of keratin 1 (Krt1) in the ducts and acini of MG explants,
accompanied by a decrease in viability and an increase in cell death in explants.
Furthermore, the SACEs treatment dose-dependently increased the levels of TNF-α,
IL-1β, and IL-6 in MG explants. The SACEs treatment induced activation of the
nuclear factor kappa B (NF-κB) and AIM2 (absent in melanoma 2)/ASC (apoptosis-
associated speck-like protein containing a caspase recruitment domain) inflammasome
signaling pathway in explants. Further investigation showed expression of the key
adipogenesis-related molecule peroxisome proliferator-activated receptor γ was
decreased after SACEs treatment. However, no change was found in the lipid
synthesis of MG explants after treatment with the SACEs. Staphylococcal
enterotoxins B (SEB) was detected in the SACEs. SEB induced the overexpression of
Krt1 and IL-1β in ducts and acini of MG explants. CONCLUSIONS. Our findings confirm
that Staphylococcus aureus induced hyperkeratinization and pro-inflammatory
cytokines expression in MG explants ducts and acini. These effects might be
mediated by SEB. Activation of the NF-κB and AIM2/ASC signaling pathway is involved
in this process. © 2021 The Authors.
AU - Chen, H.
AU - Gao, H.
AU - Xie, H. T.
AU - Liu, S. T.
AU - Huang, Y. K.
AU - Zhang, M. C.
C7 - 2777979
DB - Scopus
DO - 10.1167/iovs.62.13.11
IS - 13
KW - Meibomian gland dysfunction
Mouse meibomian gland explants
Animals
Apoptosis
Cytokines
Down-Regulation
Eye Infections, Bacterial
Inflammasomes
Male
Meibomian Gland Dysfunction
Meibomian Glands
Mice
Signal Transduction
Staphylococcal Infections
beta actin
cytokeratin 1
eosin
fat droplet
hematoxylin
interleukin 1beta
interleukin 6
Ki 67 antigen
peroxisome proliferator activated receptor gamma
Staphylococcus enterotoxin B
cytokine
inflammasome
adipogenesis
animal cell
animal experiment
Article
bacterium isolate
C57BL 6 mouse
dyskeratosis
electrochemiluminescence
enzyme activity
explant
gene overexpression
hyperkeratinization
image analysis
immunoblotting
immunofluorescence
LipidTox staining
male
meibomian gland
meibomian gland dysfunction
morphology
mouse
mouse retina explant
MTT assay
nile red staining
nonhuman
protein analysis
silver staining
staining
TUNEL assay
Western blotting
animal
apoptosis
bacterial eye infection
biosynthesis
disease model
down regulation
genetics
metabolism
microbiology
pathology
signal transduction
Staphylococcus infection
M3 - Article
PY - 2021
ST - Hyperkeratinization and proinflammatory cytokine expression in meibomian
glands induced by staphylococcus aureus
T2 - Investigative Ophthalmology and Visual Science
TI - Hyperkeratinization and proinflammatory cytokine expression in meibomian
glands induced by staphylococcus aureus
85118305396&doi=10.1167%2fiovs.62.13.11&partnerID=40&md5=032d1f3e6e17c025908cd36a9f
4f7d30
VL - 62
ID - 5218
ER -
TY - JOUR
AB - An in vitro blood-brain barrier (BBB) model being composed of co-culture with
endothelial (bEnd.3) and astrocyte-like (ALT) cells was established to evaluate the
toxicity and permeability of Ag nanoparticles (AgNPs; 8 nm) and TiO2 nanoparticles
(TiO2NPs; 6 nm and 35 nm) in normal and inflammatory central nervous system.
Lipopolysaccharide (LPS) was pre-treated to simulate the inflammatory responses.
Both AgNPs and Ag ions can decrease transendothelial electrical resistance (TEER)
value, and cause discontinuous tight junction proteins (claudin-5 and zonula
occludens-1) of BBB. However, only the Ag ions induced inflammatory cytokines to
release, and had less cell-to-cell permeability than AgNPs, which indicated that
the toxicity of AgNPs was distinct from Ag ions. LPS itself disrupted BBB, while
co-treatment with AgNPs and LPS dramatically enhanced the disruption and
permeability coefficient. On the other hand, TiO2NPs exposure increased BBB
penetration by size, and disrupted tight junction proteins without size dependence,
and many of TiO2NPs accumulated in the endothelial cells were observed. This study
provided the new insight of toxic potency of AgNPs and TiO2NPs in BBB. © 2016
Elsevier B.V.
AU - Chen, I. C.
AU - Hsiao, I. L.
AU - Lin, H. C.
AU - Wu, C. H.
AU - Chuang, C. Y.
AU - Huang, Y. J.
DB - Scopus
DO - 10.1016/j.etap.2016.09.009
KW - In vitro blood-brain barrier
Permeability
Silver ions
Titanium dioxide nanoparticles
Animals
Astrocytes
Blood-Brain Barrier
Cell Survival
Cells, Cultured
Claudin-5
Coculture Techniques
Cytokines
Endothelial Cells
Metal Nanoparticles
Mice
Silver
Titanium
Toxicity Tests
Zonula Occludens-1 Protein
claudin 5
eotaxin
gamma interferon
interleukin 10
interleukin 12p40
interleukin 12p70
interleukin 13
interleukin 17
interleukin 1alpha
interleukin 1beta
interleukin 2
interleukin 4
interleukin 5
interleukin 6
interleukin 9
lipopolysaccharide
protein ZO1
RANTES
silver nanoparticle
Cldn5 protein, mouse
cytokine
metal nanoparticle
silver
titanium
titanium dioxide
Tjp1 protein, mouse
animal cell
apoptosis
Article
astrocyte
bioaccumulation
blood brain barrier
cell viability
coculture
controlled study
cytokine release
cytotoxicity
electric resistance
endothelium cell
in vitro study
mouse
nonhuman
particle size
priority journal
animal
cell culture
cell survival
chemistry
drug effects
metabolism
permeability
procedures
toxicity testing
M3 - Article
PY - 2016
SP - 108-118
ST - Influence of silver and titanium dioxide nanoparticles on in vitro blood-
brain barrier permeability
T2 - Environmental Toxicology and Pharmacology
TI - Influence of silver and titanium dioxide nanoparticles on in vitro blood-
brain barrier permeability
84988517231&doi=10.1016%2fj.etap.2016.09.009&partnerID=40&md5=48c2e8f031099ed57b619
041302196e4
VL - 47
ID - 5465
ER -
TY - JOUR
AB - Titanium dioxide (TiO2) has a long history of application in blood contact
materials, but it often suffers from insufficient anticoagulant properties.
Recently, we have revealed the photocatalytic effect of TiO2 also induces
anticoagulant properties. However, for long-term vascular implant devices such as
vascular stents, besides anticoagulation, also anti-inflammatory, anti-hyperplastic
properties, and the ability to support endothelial repair, are desired. To meet
these requirements, here, we immobilized silver nanoparticles (AgNPs) on the
surface of TiO2 nanotubes (TiO2-NTs) to obtain a composite material with enhanced
photo-induced anticoagulant property and improvement of the other requested
properties. The photo-functionalized TiO2-NTs showed protein-fouling resistance,
causing the anticoagulant property and the ability to suppress cell adhesion. The
immobilized AgNPs increased the photocatalytic activity of TiO2-NTs to enhances its
photo-induced anticoagulant property. The AgNP density was optimized to endow the
TiO2-NTs with anti-inflammatory property, a strong inhibitory effect on smooth
muscle cells (SMCs), and low toxicity to endothelial cells (ECs). The in vivo test
indicated that the photofunctionalized composite material achieved outstanding
biocompatibility in vasculature via the synergy of photo-functionalized TiO2-NTs
and the multifunctional AgNPs, and therefore has enormous potential in the field of
cardiovascular implant devices. Our research could be a useful reference for
further designing of multifunctional TiO2 materials with high vascular
biocompatibility.
AN - WOS:000574616100004
AU - Chen, J.
AU - Dai, S.
AU - Liu, L. Y.
AU - Maitz, M. F.
AU - Liao, Y. Z.
AU - Cui, J. W.
AU - Zhao, A.
AU - Yang, P.
AU - Huang, N.
AU - Wang, Y. B.
DA - JAN
DO - 10.1016/j.bioactmat.2020.07.009
IS - 1
PY - 2021
SN - 2452-199X
SP - 45-54
ST - Photo-functionalized TiO2 nanotubes decorated with multifunctional Ag
nanoparticles for enhanced vascular biocompatibility
T2 - BIOACTIVE MATERIALS
TI - Photo-functionalized TiO2 nanotubes decorated with multifunctional Ag
VL - 6
ID - 6073
ER -
TY - JOUR
AB - Titanium dioxide (TiO2) has a long history of application in blood contact
materials, but it often suffers from insufficient anticoagulant properties.
Recently, we have revealed the photocatalytic effect of TiO2 also induces
anticoagulant properties. However, for long-term vascular implant devices such as
vascular stents, besides anticoagulation, also anti-inflammatory, anti-hyperplastic
properties, and the ability to support endothelial repair, are desired. To meet
these requirements, here, we immobilized silver nanoparticles (AgNPs) on the
surface of TiO2 nanotubes (TiO2-NTs) to obtain a composite material with enhanced
photo-induced anticoagulant property and improvement of the other requested
properties. The photo-functionalized TiO2-NTs showed protein-fouling resistance,
causing the anticoagulant property and the ability to suppress cell adhesion. The
immobilized AgNPs increased the photocatalytic activity of TiO2-NTs to enhances its
photo-induced anticoagulant property. The AgNP density was optimized to endow the
TiO2-NTs with anti-inflammatory property, a strong inhibitory effect on smooth
muscle cells (SMCs), and low toxicity to endothelial cells (ECs). The in vivo test
indicated that the photofunctionalized composite material achieved outstanding
biocompatibility in vasculature via the synergy of photo-functionalized TiO2-NTs
and the multifunctional AgNPs, and therefore has enormous potential in the field of
cardiovascular implant devices. Our research could be a useful reference for
further designing of multifunctional TiO2 materials with high vascular
biocompatibility. © 2020 [The Author/The Authors]
AU - Chen, J.
AU - Dai, S.
AU - Liu, L.
AU - Maitz, M. F.
AU - Liao, Y.
AU - Cui, J.
AU - Zhao, A.
AU - Yang, P.
AU - Huang, N.
AU - Wang, Y.
DB - Scopus
DO - 10.1016/j.bioactmat.2020.07.009
IS - 1
KW - Sliver nanoparticles(AgNPs)
Synergic effect
TiO2
UV irradiation
Vascular biocompatibility
ammonium fluoride
anticoagulant agent
gelatinase A
glycerol
heparin
horseradish peroxidase
polyvinylchloride
silver nanoparticle
silver nitrate
sodium chloride
STAT3 protein
sulfuric acid
animal cell
animal experiment
animal tissue
anticoagulation
Article
biocompatibility
blood brain barrier
cell adhesion
cell aggregation
cell culture
controlled study
emulsion
endothelium cell
hemolysis
histology
human
human cell
hydrophilicity
implantation
light intensity
light related phenomena
nonhuman
photoactivation
photolithography
photoreduction
rat
thermotherapy
thrombocyte activation
thrombocyte aggregation
thrombocyte rich plasma
thrombosis
tissue regeneration
X ray diffraction
M3 - Article
PY - 2021
SP - 45-54
ST - Photo-functionalized TiO2 nanotubes decorated with multifunctional Ag
T2 - Bioactive Materials
TI - Photo-functionalized TiO2 nanotubes decorated with multifunctional Ag
85089148815&doi=10.1016%2fj.bioactmat.2020.07.009&partnerID=40&md5=bf39474b1f1db5c3
d0e7c07862072e58
VL - 6
ID - 5352
ER -
TY - JOUR
AB - To explore the physiological mechanism of silver for the clinical application
of silver-containing biomaterials and biomedical devices, the plasma immersion ion
implantation method was used to prepare silver-doped titanium samples in this work.
The findings disclosed that with the increase of the introduced Ag amount, the
micro galvanic effects formed by silver and the titanium matrix became stronger,
which could stimulate the opening of voltage-gated calcium channels in macrophages
to facilitate Ca2+ influx. The increase of intracellular Ca2+ concentration could
induce macrophages to polarize towards the M1 phenotype by activating the Ca2+-
depen-dent PKC-NF-kappa B signaling pathway, while it was more favorable to the
polarization of M2 macrophages by improving the synthesis and secretion of calcium-
dependent PGE2. Therefore, with the increase of Ag amount, the micro-galvanic
effect was enhanced, and the anti-inflammatory responses of macrophages on samples
were improved. Furthermore, the co-cultured bone marrow mesenchymal stem cells
could inhibit the Ca2+ influx of the co-cultured macrophages to down-regulate the
Ca2+-dependent PKC-NF-kappa B signaling pathway in macrophages and secrete PGE2 to
promote the polarization of M2 macrophages, which weaken the inflammatory response.
Our results indicate that the immune response can be regulated by the micro-
galvanic effects of silver and titanium matrix, and provide new insights into
designing and developing the silver-containing biomaterials with a desirable
biological response.
AN - WOS:000728679700002
AU - Chen, L.
AU - Wang, D. H.
AU - Liu, X. D.
AU - Yan, B. C.
AU - Zhang, H. F.
AU - Zhang, X. M.
AU - Qiao, Y. Q.
AU - Qiu, J. J.
AU - Liu, X. Y.
C6 - JUL 2021
C7 - 131068
DA - JAN 15
DO - 10.1016/j.cej.2021.131068
PY - 2022
SN - 1385-8947
1873-3212
ST - Micro-galvanic effects of silver-containing titanium implants regulate the
immune responses via activating voltage-gated calcium channels in macrophages
T2 - CHEMICAL ENGINEERING JOURNAL
TI - Micro-galvanic effects of silver-containing titanium implants regulate the
immune responses via activating voltage-gated calcium channels in macrophages
VL - 428
ID - 6333
ER -
TY - JOUR
AB - Ds-block elements have been gaining increasing attention in the field of
biomaterials modification, owing to their excellent biological properties, such as
antibiosis, osteogenesis, etc. However, their function mechanisms are not well
understood and conflicting conclusions were drawn by previous studies on this
issue, which are mainly resulted from the inconsistent experimental conditions. In
this work, three most widely used ds-block elements, copper, zinc, and silver were
introduced on titanium substrate by plasma immersion ion implantation method to
investigate the rule of ds-block elements in the immune responses. Results showed
that the implanted samples could decrease the inflammatory responses compared with
Ti sample. The trend of anti-inflammatory effects of macrophages on samples was in
correlation with cellular ROS levels, which was induced by the implanted
biomaterials and positively correlated with the number of valence electrons of ds-
block elements. The co-culture experiments of macrophages and bone marrow
mesenchymal stem cells showed that these two kinds of cells could enhance the anti-
inflammation and osteogenesis of samples by the paracrine manner of PGE2. In
general, in their steady states on titanium substrate (Cu2+, Zn2+, Ag), the ds-
block elements with more valence electrons exhibit better anti-inflammatory and
osteogenic effects. Moreover, molecular biology experiments indicate that the PGE2-
related signaling pathway may contribute to the desired immunoregulation and
osteoinduction capability of ds-block elements. These findings suggest a
correlation between the number of valence electrons of ds-block elements and the
relevant biological responses, which provides new insight into the selection of
implanted ions and surface design of biomaterials. © 2020 [The Author/The Authors]
AU - Chen, L.
AU - Wang, D.
AU - Qiu, J.
AU - Zhang, X.
AU - Liu, X.
AU - Qiao, Y.
DB - Scopus
DO - 10.1016/j.bioactmat.2020.08.001
IS - 1
KW - Ds-block element
Immune response
Molecular mechanism
Osteoimmunology
Valence electron
copper
copper zinc superoxide dismutase
gamma interferon
interleukin 1 receptor
interleukin 1beta
interleukin 4
interleukin 6
prostaglandin E2
silver nanoparticle
titanium
zinc
air pouch
animal cell
animal experiment
animal tissue
Article
bone development
bone marrow derived mesenchymal stem cell
bone tissue
cell adhesion
cell density
cell proliferation
cell spreading
cell structure
coculture
cytokine production
cytotoxicity
flow cytometry
immune response
immunofluorescence
immunoregulation
inductively coupled plasma atomic emission spectrometry
macrophage
male
microscopy
mineralization
mouse
nonhuman
photoelectron spectroscopy
RAW 264.7 cell line
surface property
tumor associated leukocyte
zeta potential
M3 - Article
PY - 2021
SP - 191-207
ST - Synergistic effects of immunoregulation and osteoinduction of ds-block
elements on titanium surface
TI - Synergistic effects of immunoregulation and osteoinduction of ds-block
elements on titanium surface
85089337798&doi=10.1016%2fj.bioactmat.2020.08.001&partnerID=40&md5=5ec9743ba308f8e0
7a17883e6b9bc131
VL - 6
ID - 5354
ER -
TY - JOUR
AB - The adverse outcomes of silver nanoparticles (AgNPs) on pregnancy have been
studied in murine animals. However, the potential toxicity of AgNPs to immune
balance, which is essential for maintaining a normal pregnancy, still requires
further exploration. Therefore, this study assessed the effect of AgNPs on the
immune balance during gestation time. Pregnant mice were given a dose of 1 mg/kg of
AgNPs and silver ion on gestation days 3.5 to 9.5 by tail vein injection. Results
showed that the AgNPs and silver ion decreased the number of CD4(+)CD25(+) Treg
cells which were the important cells in the immune system, thereby disrupting the
balance of normal immune tolerance function, activated the inflammatory responses,
together with the reductive production of placental immunoregulatory genes, and the
expression of inflammatory factors in the placenta in the Ag-treated groups
increased. These effects increased the absorption rate. Furthermore, the
inflammatory signaling pathway p38MAPK/AP-1/MMP-9 in the placenta was activated,
indicating that Ag induced inflammation through this signaling pathway. All results
indicated that undesirable pregnancy outcome caused by AgNPs could be happened by
stimulating immunological dysfunction. Therefore, the potential risks to
embryogenesis exposure to AgNPs that caused immune imbalance should be given
sufficient attention.
AN - WOS:000538805800001
AU - Chen, L.
AU - Wu, H. F.
AU - Le, L. L.
AU - Yang, P. F.
AU - Fu, F.
AU - Liu, W. T.
AU - Xu, H. Y.
C6 - JUN 2020
DA - NOV
DO - 10.1002/tox.22981
IS - 11
PY - 2020
SN - 1520-4081
1522-7278
SP - 1161-1169
ST - Exposure to silver nanoparticles induces immunological dysfunction in
pregnant mice
T2 - ENVIRONMENTAL TOXICOLOGY
TI - Exposure to silver nanoparticles induces immunological dysfunction in
pregnant mice
VL - 35
ID - 5925
ER -
TY - JOUR
AB - The adverse outcomes of silver nanoparticles (AgNPs) on pregnancy have been
studied in murine animals. However, the potential toxicity of AgNPs to immune
balance, which is essential for maintaining a normal pregnancy, still requires
further exploration. Therefore, this study assessed the effect of AgNPs on the
immune balance during gestation time. Pregnant mice were given a dose of 1 mg/kg of
AgNPs and silver ion on gestation days 3.5 to 9.5 by tail vein injection. Results
showed that the AgNPs and silver ion decreased the number of CD4+CD25+ Treg cells
which were the important cells in the immune system, thereby disrupting the balance
of normal immune tolerance function, activated the inflammatory responses, together
with the reductive production of placental immunoregulatory genes, and the
expression of inflammatory factors in the placenta in the Ag-treated groups
increased. These effects increased the absorption rate. Furthermore, the
inflammatory signaling pathway p38MAPK/AP-1/MMP-9 in the placenta was activated,
indicating that Ag induced inflammation through this signaling pathway. All results
indicated that undesirable pregnancy outcome caused by AgNPs could be happened by
stimulating immunological dysfunction. Therefore, the potential risks to
embryogenesis exposure to AgNPs that caused immune imbalance should be given
sufficient attention. © 2020 Wiley Periodicals LLC.
AU - Chen, L.
AU - Wu, H.
AU - Le, L.
AU - Yang, P.
AU - Fu, F.
AU - Liu, W.
AU - Xu, H.
DB - Scopus
DO - 10.1002/tox.22981
IS - 11
KW - CD4+CD25+ Treg cells
immune dysfunction
inflammation
Animals
Embryonic Development
Female
Immune System
Metal Nanoparticles
Mice
Placenta
Pregnancy
Silver
Animalia
Murinae
Mus
Mammals
Metal ions
Metal nanoparticles
Obstetrics
CD4 antigen
gamma interferon
gelatinase B
indoleamine 2,3 dioxygenase
interleukin 10
interleukin 2 receptor alpha
interleukin 6
messenger RNA
silver nanoparticle
suppressor of cytokine signaling 3
transcription factor AP 1
metal nanoparticle
silver
Absorption rates
Immune tolerances
Immunoregulatory genes
Inflammatory signaling
Signaling pathways
Tail vein injections
ecotoxicology
embryonic development
gene expression
immune response
immunoassay
nanoparticle
pregnancy
rodent
signaling
animal experiment
animal tissue
Article
CD4+ CD25+ T lymphocyte
controlled study
embryo development
female
fetus
gestational age
histopathology
immunological tolerance
immunoregulation
male
maternal exposure
maternal serum
mouse
nanotoxicology
nonhuman
placenta
pregnancy outcome
priority journal
regulatory T lymphocyte
signal transduction
animal
drug effect
immune system
M3 - Article
PY - 2020
SP - 1161-1169
ST - Exposure to silver nanoparticles induces immunological dysfunction in
pregnant mice
T2 - Environmental Toxicology
TI - Exposure to silver nanoparticles induces immunological dysfunction in
pregnant mice
85086095697&doi=10.1002%2ftox.22981&partnerID=40&md5=068fd69e936ab1b95d74c1eb2874ed
c7
VL - 35
ID - 5308
ER -
TY - JOUR
AB - Introduction: Increased use of silver nanoparticles (AgNPs) has raised
concerns that AgNPs may induce toxic effects. In vitro studies of cell monolayers
and in vivo studies have produced conflicting results. The inconsistency of these
results has been mainly due to limitations of two-dimensional (2D) monolayer cell
systems. Methods: A three-dimensional (3D) epidermal model called EpiKutis (R),
which exhibits good tissue viability and barrier function was developed. The
cytotoxicity of AgNPs against EpiKutis was compared to that against 2D
keratinocytes at equivalent AgNPs doses (0.035, 0.07, 0.14, 0.28, and 0.56 ng per
cell). The amount and distribution of AgNPs in the 3D EpiKutis and 2D keratinocytes
after exposure were determined. The toxic mechanisms of AgNPs, such as oxidative
stress and production of pro-inflammatory cytokines, were investigated. Results:
The results demonstrated that cell viability was greater than 80% and lactate
dehydrogenase (LDH) release did not increase even at the highest dose of AgNPs in
EpiKutis. In contrast, treatment of 2D keratinocytes with AgNPs resulted in dose-
dependent decrease in cell viability from 63% to 11%, and a dose-dependent increase
in LDH release from 8% to 16%. Cytotoxicity of AgNPs in 2D keratinocytes was
related to oxidative damage and inflammation, as evidenced by increased levels of
reactive oxygen species (ROS), malondialdehyde (MDA), IL-1 alpha, IL-6, and IL-8.
In addition, levels of superoxide dismutase (SOD) were decreased. EpiKutis treated
with AgNPs did not exhibit increased oxidative damage or inflammation, which may
have been due to the barrier properties of the 3D structure, resulting in reduced
penetration of AgNPs. At equivalent per cell doses, total silver penetration into
EpiKutis was 0.9 +/- 0.1%, and total silver penetration into 2D keratinocytes was
8.8 +/- 0.6% detected by ICP-MS. The penetration and distribution of AgNPs in 2D
keratinocytes were confirmed by the TEM-EDS analysis, which was not found in the 3D
EpiKutis. These results showed that AgNPs penetrated EpiKutis to a lesser degree
than they penetrated 2D keratinocytes, which suggested that EpiKutis exhibited
significant barrier function. Discussion: The results of this study showed that
AgNP toxicity should be evaluated using 3D epidermal models, which may provide
better estimates of in vivo conditions than 2D models. The EpiKutis model may be an
ideal model for assessment of nanotoxicity.
AN - WOS:000502016400001
AU - Chen, L.
AU - Wu, M. Y.
AU - Jiang, S.
AU - Zhang, Y. Y.
AU - Li, R. Z.
AU - Lu, Y. B.
AU - Liu, L.
AU - Wu, G.
AU - Liu, Y.
AU - Xie, L. M.
AU - Xu, L. M.
DO - 10.2147/IJN.S225451
PY - 2019
SN - 1178-2013
SP - 9707-9719
ST - Skin Toxicity Assessment of Silver Nanoparticles in a 3D Epidermal Model
Compared to 2D Keratinocytes
TI - Skin Toxicity Assessment of Silver Nanoparticles in a 3D Epidermal Model
Compared to 2D Keratinocytes
VL - 14
ID - 5877
ER -
TY - JOUR
AB - Introduction: Increased use of silver nanoparticles (AgNPs) has raised
concerns that AgNPs may induce toxic effects. In vitro studies of cell monolayers
and in vivo studies have produced conflicting results. The inconsistency of these
results has been mainly due to limitations of two-dimensional (2D) monolayer cell
systems. Methods: A three-dimensional (3D) epidermal model called EpiKutis®, which
exhibits good tissue viability and barrier function was developed. The cytotoxicity
of AgNPs against EpiKutis was compared to that against 2D keratinocytes at
equivalent AgNPs doses (0.035, 0.07, 0.14, 0.28, and 0.56 ng per cell). The amount
and distribution of AgNPs in the 3D EpiKutis and 2D keratinocytes after exposure
were determined. The toxic mechanisms of AgNPs, such as oxidative stress and
production of pro-inflammatory cytokines, were investigated. Results: The results
demonstrated that cell viability was greater than 80% and lactate dehydrogenase
(LDH) release did not increase even at the highest dose of AgNPs in EpiKutis. In
contrast, treatment of 2D keratinocytes with AgNPs resulted in dose-dependent
decrease in cell viability from 63% to 11%, and a dose-dependent increase in LDH
release from 8% to 16%. Cytotoxicity of AgNPs in 2D keratinocytes was related to
oxidative damage and inflammation, as evidenced by increased levels of reactive
oxygen species (ROS), malondialdehyde (MDA), IL-1α, IL-6, and IL-8. In addition,
levels of superoxide dismutase (SOD) were decreased. EpiKutis treated with AgNPs
did not exhibit increased oxidative damage or inflammation, which may have been due
to the barrier properties of the 3D structure, resulting in reduced penetration of
AgNPs. At equivalent per cell doses, total silver penetration into EpiKutis was 0.9
± 0.1%, and total silver penetration into 2D keratinocytes was 8.8 ± 0.6% detected
by ICP-MS. The penetration and distribution of AgNPs in 2D keratinocytes were
confirmed by the TEM-EDS analysis, which was not found in the 3D EpiKutis. These
results showed that AgNPs penetrated EpiKutis to a lesser degree than they
penetrated 2D keratinocytes, which suggested that EpiKutis exhibited significant
barrier function. Discussion: The results of this study showed that AgNP toxicity
should be evaluated using 3D epidermal models, which may provide better estimates
of in vivo conditions than 2D models. The EpiKutis model may be an ideal model for
assessment of nanotoxicity. © 2019 Chen et al.
AU - Chen, L.
AU - Wu, M.
AU - Jiang, S.
AU - Zhang, Y.
AU - Li, R.
AU - Lu, Y.
AU - Liu, L.
AU - Wu, G.
AU - Liu, Y.
AU - Xie, L.
AU - Xu, L.
DB - Scopus
DO - 10.2147/IJN.S225451
KW - 3D epidermal model
AgNPs
Cytotoxicity
EpiKutis®
Keratinocytes
Oxidative stress
Cell Culture Techniques
Cell Survival
Epidermis
Humans
Male
Malondialdehyde
Metal Nanoparticles
Oxidative Stress
Silver
Skin
Superoxide Dismutase
Toxicity Tests
interleukin 1alpha
interleukin 6
interleukin 8
malonaldehyde
silver nanoparticle
metal nanoparticle
silver
Article
biological model
cell culture
cell viability
controlled study
cytokine production
cytokine release
enzyme release
human
human cell
human tissue
in vivo study
inflammation
intermethod comparison
keratinocyte
oxidative stress
physical chemistry
skin toxicity
toxicity testing
cell culture technique
cell survival
chemistry
comparative study
cytology
drug effect
epidermis
male
metabolism
procedures
skin
M3 - Article
PY - 2019
SP - 9707-9719
ST - Skin toxicity assessment of silver nanoparticles in a 3D epidermal model
compared to 2D keratinocytes
TI - Skin toxicity assessment of silver nanoparticles in a 3D epidermal model
compared to 2D keratinocytes
85076841592&doi=10.2147%2fIJN.S225451&partnerID=40&md5=e257e00e7f463730220f5e0a9b8d
2bb8
VL - 14
ID - 5444
ER -
TY - JOUR
AB - Alveolar bone loss is a common problem that affects dental implant placement.
A barrier between the bone substitute and gingiva that can prevent fibro-tissue
ingrowth, bacterial infection and induce bone formation is a key factor in
improving the success of alveolar ridge reconstruction. This study aims to develop
a bioactive collagen barrier material for guided bone regeneration, that is coupled
with anti-bacterial and anti-inflammatory properties. We have evaluated two silver
coating methods and found controllable and precise coating achieved by sonication
compared with sputtering. The optimized AgNP-coated collagen membrane exhibited
excellent anti-bacterial effects against Staphylococcus aureus (S. aureus) and
Pseudomonas aeruginosa (P. aeruginosa) with limited cellular toxicity. It also
displayed effective anti-inflammatory effects by reducing the expression and
release of inflammatory cytokines including IL-6 and TNF-alpha. Additionally, AgNP-
coated collagen membranes were able to induce osteogenic differentiation of
mesenchymal stem cells that guide bone regeneration. These findings demonstrate the
potential application of AgNP- coated collagen membranes to prevent infection after
bone graft introduction in alveolar ridge reconstruction.
AN - WOS:000446194300004
AU - Chen, P. L.
AU - Wu, Z. G.
AU - Leung, A.
AU - Chen, X. B.
AU - Landao-Bassonga, E.
AU - Gao, J. J.
AU - Chen, L. Z.
AU - Zheng, M.
AU - Yao, F.
AU - Yang, H.
AU - Lidgren, L.
AU - Allan, B.
AU - Liu, Y. N.
AU - Wang, T.
AU - Zheng, M. H.
C7 - 065014
DA - NOV
DO - 10.1088/1748-605X/aae15b
IS - 6
PY - 2018
SN - 1748-6041
1748-605X
ST - Fabrication of a silver nanoparticle-coated collagen membrane with anti-
bacterial and anti-inflammatory activities for guided bone regeneration
T2 - BIOMEDICAL MATERIALS
TI - Fabrication of a silver nanoparticle-coated collagen membrane with anti-
VL - 13
ID - 5944
ER -
TY - JOUR
AB - Alveolar bone loss is a common problem that affects dental implant placement.
A barrier between the bone substitute and gingiva that can prevent fibro-tissue
ingrowth, bacterial infection and induce bone formation is a key factor in
improving the success of alveolar ridge reconstruction. This study aims to develop
a bioactive collagen barrier material for guided bone regeneration, that is coupled
with anti-bacterial and anti-inflammatory properties. We have evaluated two silver
coating methods and found controllable and precise coating achieved by sonication
compared with sputtering. The optimized AgNP-coated collagen membrane exhibited
excellent anti-bacterial effects against Staphylococcus aureus (S. aureus) and
Pseudomonas aeruginosa (P. aeruginosa) with limited cellular toxicity. It also
displayed effective anti-inflammatory effects by reducing the expression and
release of inflammatory cytokines including IL-6 and TNF-alpha. Additionally, AgNP-
coated collagen membranes were able to induce osteogenic differentiation of
mesenchymal stem cells that guide bone regeneration. These findings demonstrate the
potential application of AgNP-coated collagen membranes to prevent infection after
bone graft introduction in alveolar ridge reconstruction. © 2018 IOP Publishing
Ltd.
AU - Chen, P.
AU - Wu, Z.
AU - Leung, A.
AU - Chen, X.
AU - Landao-Bassonga, E.
AU - Gao, J.
AU - Chen, L.
AU - Zheng, M.
AU - Yao, F.
AU - Yang, H.
AU - Lidgren, L.
AU - Allan, B.
AU - Liu, Y.
AU - Wang, T.
C7 - 065014
DB - Scopus
DO - 10.1088/1748-605X/aae15b
IS - 6
KW - anti-bacterial
anti-inflammation
collagen
guide bone regeneration
Alveolar Process
Animals
Bone Regeneration
Bone Substitutes
Cell Survival
Collagen
Dental Implants
Gingiva
Guided Tissue Regeneration
Guided Tissue Regeneration, Periodontal
Interleukin-6
Mesenchymal Stem Cells
Metal Nanoparticles
Mice
Mice, Inbred C3H
Osseointegration
Osteogenesis
Silver
Surface Properties
Bacteria
Cell culture
Coatings
Dental prostheses
Metal nanoparticles
Stem cells
alkaline phosphatase bone isoenzyme
interleukin 6
nanocoating
osteopontin
silver nanoparticle
transcription factor RUNX2
antiinfective agent
biocompatible coated material
metal nanoparticle
silver
Anti-bacterial
Anti-inflammation
Anti-inflammatory effects
Bone regeneration
Guided bone regeneration
Osteogenic differentiation
36b4 gene
animal cell
Article
bone development
bone regeneration
chemical procedures
controlled study
cytokine release
cytotoxicity
drug efficacy
evaluation study
housekeeping gene
in vitro study
material coating
mouse
nanofabrication
nanopharmaceutics
nanotoxicology
nonhuman
protein expression
RAW 264.7 cell line
reaction optimization
sputtering technique
ultrasound
alveolar bone
animal
bone prosthesis
C3H mouse
cell survival
chemistry
drug effect
gingiva
metabolism
osseointegration
periodontal guided tissue regeneration
physiology
procedures
surface property
tissue regeneration
tooth implant
Bone
M3 - Article
PY - 2018
ST - Fabrication of a silver nanoparticle-coated collagen membrane with anti-
T2 - Biomedical Materials (Bristol)
TI - Fabrication of a silver nanoparticle-coated collagen membrane with anti-
85054399200&doi=10.1088%2f1748-605X
%2faae15b&partnerID=40&md5=8e47c0f9a11b54e28801ad64c40cb576
VL - 13
ID - 5369
ER -
TY - JOUR
AB - Lanthanide-doped sodium yttrium fluoride (NaYF4) nanoparticles exhibit novel
optical properties which make them be widely used in various fields. The extensive
applications increase the chance of human exposure to these nanoparticles and thus
raise deep concerns regarding their riskiness. In the present study, we have
synthesized europium doped NaYF4 (NaYF4:Eu3+) nanoparticles with three diameters
and used endothelial cells (ECs) as a cell model to explore the potential toxic
effect. The cell viability, cytomembrane integrity, cellular uptake, intracellular
localization, intracellular reactive oxygen species (ROS), mitochondrial membrane
potential (MMP), apoptosis detection, caspase-3 activity and expression of
inflammatory gene were studied. The results indicated that these nanoparticles
could be uptaken into ECs and decrease the cell viability, induce the intracellular
lactate dehydrogenase (LDH) release, increase the ROS level, and decrease the cell
MMP in a size-dependent manner. Besides that, the cells were suffered to apoptosis
with the caspase-3 activation, and the inflammation specific gene expressions
(ICAM1 and VCAM1) were also increased. Our results suggest that the damage pathway
may be related to the ROS generation and mitochondrial damage. The results provide
novel evidence to elucidate their toxicity mechanisms and may be helpful for more
rational applications of these compounds in the future. (C) 2014 Elsevier B.V. All
rights reserved.
AN - WOS:000342529000041
AU - Chen, S. Z.
AU - Zhang, C. M.
AU - Jia, G.
AU - Duan, J. L.
AU - Wang, S. X.
AU - Zhang, J. C.
DA - OCT 1
DO - 10.1016/j.msec.2014.07.029
PY - 2014
SN - 0928-4931
1873-0191
SP - 330-342
ST - Size-dependent cytotoxicity of europium doped NaYF4 nanoparticles in
endothelial cells
TI - Size-dependent cytotoxicity of europium doped NaYF4 nanoparticles in
endothelial cells
VL - 43
ID - 6142
ER -
TY - JOUR
AB - Rationale: Inflammatory osteolysis, characterized by abundant immune cell
infiltration and osteoclast (OC) formation, is a common complication induced by
bacterial products and/or wear particles at the bone-prosthesis interface that
severely reduces long-term stability after implantation. Molecular nanoclusters are
ultrasmall particles with unique physicochemical and biological properties that
have great potential as theranostic agents for treating inflammatory
diseases.Methods: In this study, heterometallic PtAu2 nanoclusters with sensitive
nitric oxide-responsive phosphorescence turn-on characteristics and strong binding
interactions with cysteine were designed, making them desirable candidates for the
treatment of inflammatory osteolysis.Results: PtAu2 clusters exhibited satisfactory
biocompatibility and cellular uptake behavior, with potent antiinflammatory and
anti-OC activities in vitro. In addition, PtAu2 clusters alleviated
lipopolysaccharide-induced calvarial osteolysis in vivo and activated nuclear
factor erythroid 2-related factor 2 (Nrf2) expression by disrupting its association
with Kelch-like ECH-associated protein 1 (Keap1), thereby upregulating the
expression of endogenous anti-inflammatory and anti-oxidative products.Conclusion:
Through the rational design of novel heterometallic nanoclusters that activate the
endogenous anti-inflammatory system, this study provides new insights into the
development of multifunctional molecular therapeutic agents for inflammatory
osteolysis and other inflammatory diseases.
AN - WOS:000927791500003
AU - Chen, X. Z.
AU - Cao, X. K.
AU - Zheng, D. S.
AU - Li, C.
AU - Chen, Y.
AU - Kong, K. Y.
AU - Xu, W. F.
AU - Shi, B.
AU - Chen, X. W.
AU - Dai, F. R.
AU - Zhang, S. Y.
DO - 10.7150/thno.80514
IS - 3
PY - 2023
SN - 1838-7640
SP - 1010-1027
ST - Ultrasmall PtAu2 nanoclusters activate endogenous anti-inflammatory and anti-
oxidative systems to prevent inflammatory osteolysis
T2 - THERANOSTICS
TI - Ultrasmall PtAu2 nanoclusters activate endogenous anti-inflammatory and anti-
oxidative systems to prevent inflammatory osteolysis
VL - 13
ID - 6164
ER -
TY - JOUR
AB - Bacterial infections are often treated inadequately. Sepsis, being one of its
most severe forms, is a multi-layered, life-threatening syndrome induced by rampant
immune responses, like cytokine storms, that leads to high morbidity and death of
infected patients. Particularly, the current increment in resistant bacterial
strains and the lack of creative antibiotics to counter such menace are central
reasons to the worsening of the situation. To avoid the said crisis, the
antimicrobial peptides (AMPs) were used to target cell wall components, such as
lipopoly-saccharides (LPS), seems to have the most promise. These combine the
ability of broad-spectrum bactericidal activity with low potential for induction of
resistance. Inhibition of cytokine storms induced by activated immune cells has
been considered a feasible treatment for in sepsis. One of the therapeutic
approaches widely utilized in inducing apoptosis in inflammatory cells is the use
of tumor necrosis factor (TNF)-related apoptosisinducing ligands (TRAIL), which
trigger an extrinsic apoptotic pathway via death receptors. Herein, we report TRAIL
encapsulated in a bactericidal polypeptide-crosslinked nanogel that suppressed
Klebsiella pneumoniae infection and overactive macrophages. Of interest, nanogel
and TRAIL-nanogel treatments were more toxic towards LPS-activated cells than to
naive cells in cell viability assays. Treatment with TRAIL-nanogel significantly
prolonged survival in septic mice and reduced bacterial numbers in circulation. As
such, TRAIL-nanogel may be promising as a therapeutic agent for treating bacteria-
infected diseases.
AN - WOS:000472241700009
AU - Chen, Y. F.
AU - Chen, G. Y.
AU - Chang, C. H.
AU - Su, Y. C.
AU - Chen, Y. C.
AU - Jiang, Y. S.
AU - Jan, J. S.
DA - SEP
DO - 10.1016/j.msec.2019.04.023
PY - 2019
SN - 0928-4931
1873-0191
SP - 85-95
ST - TRAIL encapsulated to polypeptide-crosslinked nanogel exhibits increased
anti-inflammatory activities in Klebsiella pneumoniae-induced sepsis treatment
T2 - MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
TI - TRAIL encapsulated to polypeptide-crosslinked nanogel exhibits increased
anti-inflammatory activities in Klebsiella pneumoniae-induced sepsis treatment
VL - 102
ID - 6244
ER -
TY - JOUR
AB - A reliable electrophysiological electrode interface (EEI) between
bioelectronic devices and biological tissues is indispensable to achieve the high
fidelity recording of bioelectricity. However, there is an inherent tradeoff among
EEI's electrochemical characteristics, mechanical properties and biocompatibility
when considering the desired nanostructure and optimum composition. Here, we
proposed a mechanically matched, highly conductive and biocompatible EEI, a tissue-
like metal-doped hydrogel which could enable excellent electro-biosensing, by
bringing disulfide modified silver nanowires into difunctional
hyaluronan/carboxymethyl chitosan composite. The intensity of cortical signals at
specific frequency domain recorded by the hydrogel-based EEI is doubled, which is
significant for the diagnosis of epilepsy. Furthermore, the natural gel matrix
could lead to seamless bio-integration between EEI and the tissue site of interest,
minimizing signal dissipation without causing obvious inflammatory response.
Overall, the EEI we designed contributes to improving tissue-device integration as
well as bioelectronic device's performance and further leads to more effective
human-computer interfaces.
AN - WOS:000860754400005
AU - Chen, Z. H.
AU - Liu, X. Y.
AU - Ding, J.
AU - Tian, Y.
AU - Zhang, Y. S.
AU - Wei, D.
AU - Sun, J.
AU - Luo, F.
AU - Zhou, L. X.
AU - Fan, H. S.
C6 - AUG 2022
C7 - 119923
DA - NOV 15
DO - 10.1016/j.carbpol.2022.119923
PY - 2022
SN - 0144-8617
1879-1344
ST - Tissue-like electrophysiological electrode interface construction by multiple
crosslinked polysaccharide-based hydrogel
T2 - CARBOHYDRATE POLYMERS
TI - Tissue-like electrophysiological electrode interface construction by multiple
VL - 296
ID - 6359
ER -
TY - JOUR
AB - A reliable electrophysiological electrode interface (EEI) between
bioelectronic devices and biological tissues is indispensable to achieve the high
fidelity recording of bioelectricity. However, there is an inherent tradeoff among
EEI's electrochemical characteristics, mechanical properties and biocompatibility
when considering the desired nanostructure and optimum composition. Here, we
proposed a mechanically matched, highly conductive and biocompatible EEI, a tissue-
like metal-doped hydrogel which could enable excellent electro-biosensing, by
bringing disulfide modified silver nanowires into difunctional
hyaluronan/carboxymethyl chitosan composite. The intensity of cortical signals at
specific frequency domain recorded by the hydrogel-based EEI is doubled, which is
significant for the diagnosis of epilepsy. Furthermore, the natural gel matrix
could lead to seamless bio-integration between EEI and the tissue site of interest,
minimizing signal dissipation without causing obvious inflammatory response.
Overall, the EEI we designed contributes to improving tissue-device integration as
well as bioelectronic device's performance and further leads to more effective
human-computer interfaces. © 2022
AU - Chen, Z.
AU - Liu, X.
AU - Ding, J.
AU - Tian, Y.
AU - Zhang, Y.
AU - Wei, D.
AU - Sun, J.
AU - Luo, F.
AU - Zhou, L.
AU - Fan, H.
C7 - 119923
DB - Scopus
DO - 10.1016/j.carbpol.2022.119923
KW - Bioelectronics
Conductive hydrogel
Electrophysiological electrode interface
Hyaluronic acid
Tissue adhesion
Electrodes
Humans
Hydrogels
Nanowires
Polysaccharides
Silver
Biocompatibility
Biomechanics
Electrophysiology
Frequency domain analysis
Sulfur compounds
nanowire
polysaccharide
silver
Bioelectronic
Bioelectronic device
Biological tissues
Crosslinked
Electrochemical characteristics
Electrode interface
High-fidelity
chemistry
electrode
human
hydrogel
Tissue
M3 - Article
PY - 2022
ST - Tissue-like electrophysiological electrode interface construction by multiple
T2 - Carbohydrate Polymers
TI - Tissue-like electrophysiological electrode interface construction by multiple
85135379566&doi=10.1016%2fj.carbpol.2022.119923&partnerID=40&md5=e09f317118322bf508
b643fd84ae3c3e
VL - 296
ID - 5045
ER -
TY - JOUR
AB - Today, earphones have almost been owned by everyone. However, wearing
earphones for a long time can cause two serious problems: (1) irreversible damage
to hearing; (2) rapid proliferation of bacteria in the ear canal. Herein, an
earphone modification strategy is developed for the first time, to reduce hearing
loss and inhibit bacteria simultaneously. This earphone is equipped with a high
purity yellow light (YL) light-emitting diode chip developed by our university.
Then, the surface of the earphone is loaded with porous zinc oxide and silver
nanoparticle composite material (ZnO-Ag) that can respond to the YL. Under the
excitation of YL, the porous ZnO-Ag can release reactive oxygen species with strong
antibacterial activity. More importantly, we discover that YL can reduce the
expression of matrix metalloproteinase-3, the secretion of inflammatory factors,
and apoptosis in the cochlea, thereby effectively reduce hearing loss. [Figure not
available: see fulltext.] © 2022, Tsinghua University Press.
AU - Cheng, H.
AU - Liu, H.
AU - Liu, Z.
AU - Xu, Z.
AU - Liu, X.
AU - Jia, S.
AU - He, C.
AU - Liu, S.
AU - Zhang, J.
AU - Wang, X.
DB - Scopus
DO - 10.1007/s12274-022-4240-7
IS - 7
KW - antibacterial activity
earphone
hearing loss
yellow light
ZnO
Bacteria
Cell death
Zinc oxide
Diode chips
Ear canal
Hearing loss
High purity
Irreversible damage
Lightemitting diode
Material-based
Responsive materials
Yellow light
Audition
M3 - Article
PY - 2022
SP - 6297-6305
ST - Yellow responsive material based modification to reduce earphone induced
Infection and hearing loss
T2 - Nano Research
TI - Yellow responsive material based modification to reduce earphone induced
Infection and hearing loss
85127634912&doi=10.1007%2fs12274-022-4240-
7&partnerID=40&md5=d7afd9124bb1ad85e3fb6fa8324c5eab
VL - 15
ID - 5140
ER -
TY - JOUR
AB - Tendon injuries occur commonly in sports and workplace. Tendon-derived stem
cells (TDSCs) have great potential for tendon healing because they can
differentiate into functional tenocytes. To grow TDSCs properly in vivo, a scaffold
is needed. Silver nanoparticles (AgNPs) have been used in a range of biomedical
applications for their anti-bacterial and -inflammatory effects. AgNPs are
therefore expected to be a good scaffolding coating material for tendon
engineering. Yet, their cytotoxicity in TDSCs remains unknown. Moreover, their
sublethal effects were mysterious in TDSCs. In our study, decahedral AgNPs (43.5 nm
in diameter) coated with polyvinylpyrrolidone (PVP) caused a decrease in TDSCs'
viability beginning at 37.5 mu g ml(-1) but showed non-cytotoxic effects at
concentrations below 18.8 mu g ml(-1). Apoptosis was observed in the TDSCs when
higher doses of AgNPs (75-150 mu g ml(-1)) were used. Mechanistically, AgNPs
induced reactive oxygen species (ROS) formation and mitochondrial membrane
potential (MMP) depolarization, resulting in apoptosis. Interestingly, treating
TDSCs with N-acetyl-L-cysteine (NAC) antioxidant significantly antagonized the ROS
formation, MMP depolarization and apoptosis indicating that ROS accumulation was a
prominent mediator in the AgNP-induced cytotoxicity. On the other hand, AgNPs
inhibited the tendon markers' mRNA expression (0-15 mu g ml(-1)), proliferation and
clonogenicity (0-15 mu g ml(-1)) in TDSCs under non-cytotoxic concentrations. Taken
together, we have reported here for the first time that the decahedral AgNPs are
cytotoxic to rat TDSCs and their sublethal effects are also detrimental to stem
cells' proliferation and tenogenic differentiation. Therefore, AgNPs are not a good
scaffolding coating material for tendon engineering.
AN - WOS:000366832700028
AU - Cheung, T. S.
AU - Lau, P. M.
AU - Lu, H. F.
AU - Ho, H. P.
AU - Lui, P. P. Y.
AU - Kong, S. K.
DO - 10.1039/c5tx00349k
IS - 1
PY - 2016
SN - 2045-452X
2045-4538
SP - 318-330
ST - Cytotoxic and sublethal effects of silver nanoparticles on tendon-derived
stem cells - implications for tendon engineering
T2 - TOXICOLOGY RESEARCH
TI - Cytotoxic and sublethal effects of silver nanoparticles on tendon-derived
stem cells - implications for tendon engineering
VL - 5
ID - 6078
ER -
TY - JOUR
AB - Tendon injuries occur commonly in sports and workplace. Tendon-derived stem
cells (TDSCs) have great potential for tendon healing because they can
differentiate into functional tenocytes. To grow TDSCs properly in vivo, a scaffold
is needed. Silver nanoparticles (AgNPs) have been used in a range of biomedical
applications for their anti-bacterial and -inflammatory effects. AgNPs are
therefore expected to be a good scaffolding coating material for tendon
engineering. Yet, their cytotoxicity in TDSCs remains unknown. Moreover, their
sublethal effects were mysterious in TDSCs. In our study, decahedral AgNPs (43.5 nm
in diameter) coated with polyvinylpyrrolidone (PVP) caused a decrease in TDSCs'
viability beginning at 37.5 μg ml-1 but showed non-cytotoxic effects at
concentrations below 18.8 μg ml-1. Apoptosis was observed in the TDSCs when higher
doses of AgNPs (75-150 μg ml-1) were used. Mechanistically, AgNPs induced reactive
oxygen species (ROS) formation and mitochondrial membrane potential (MMP)
depolarization, resulting in apoptosis. Interestingly, treating TDSCs with N-
acetyl-l-cysteine (NAC) antioxidant significantly antagonized the ROS formation,
MMP depolarization and apoptosis indicating that ROS accumulation was a prominent
mediator in the AgNP-induced cytotoxicity. On the other hand, AgNPs inhibited the
tendon markers' mRNA expression (0-15 μg ml-1), proliferation and clonogenicity (0-
15 μg ml-1) in TDSCs under non-cytotoxic concentrations. Taken together, we have
reported here for the first time that the decahedral AgNPs are cytotoxic to rat
TDSCs and their sublethal effects are also detrimental to stem cells' proliferation
and tenogenic differentiation. Therefore, AgNPs are not a good scaffolding coating
material for tendon engineering. © The Royal Society of Chemistry 2016.
AU - Cheung, T. S.
AU - Lau, P. M.
AU - Lu, H.
AU - Ho, H. P.
AU - Lui, P. P. Y.
AU - Kong, S. K.
DB - Scopus
DO - 10.1039/c5tx00349k
IS - 1
KW - acetylcysteine
biological marker
messenger RNA
povidone
silver nanoparticle
animal cell
apoptosis
Article
cell engineering
cell growth
cell viability
clonogenesis
controlled study
cytotoxicity
gene expression
material coating
membrane depolarization
nonhuman
particle size
priority journal
rat
stem cell
tendon derived stem cell
M3 - Article
PY - 2015
SP - 318-330
ST - Cytotoxic and sublethal effects of silver nanoparticles on tendon-derived
stem cells - Implications for tendon engineering
T2 - Toxicology Research
TI - Cytotoxic and sublethal effects of silver nanoparticles on tendon-derived
stem cells - Implications for tendon engineering
84951856775&doi=10.1039%2fc5tx00349k&partnerID=40&md5=49d03f1f0c08e9c0ee590e1e13161
890
VL - 5
ID - 5564
ER -
TY - JOUR
AB - Background The use of nanomaterials is constantly increasing in electronics,
cosmetics, food additives, and is emerging in advanced biomedical applications such
as theranostics, bio-imaging and therapeutics. However their safety raises concerns
and requires appropriate methods to analyze their fate in vivo. Scope of review In
this review, we describe the current knowledge about the toxicity of labile metal
(ZnO, CuO and Ag) nanoparticles (NPs) both at the organism and cellular levels, and
describe the pathways that are triggered to maintain cellular homeostasis. We also
describe advanced elemental imaging approaches to analyze intracellular NP fate.
Finally, we open the discussion by presenting recent developments in terms of
synthesis and applications of Ag and CuO NPs. Major conclusions Labile metal
nanoparticles (MeNPs) release metal ions that trigger a cellular response involving
biomolecules binding to the ions followed by regulation of the redox balance. In
addition, specific mechanisms are set up by the cell in response to physiological
ions such as Cu(I) and Zn(II). Among all types of NPs, labile MeNPs induce the
strongest inflammatory responses which are most probably due to the combined
effects of the NPs and of its released ions. Interestingly, recent developments in
imaging technologies enable the intracellular visualization of both the NPs and
their ions and promise new insights into nanoparticle fate and toxicity. General
significance The exponential use of nanotechnologies associated with the
difficulties of assessing their impact on health and the environment has prompted
scientists to develop novel methodologies to characterize these nanoobjects in a
biological context. © 2016 Elsevier B.V.
AU - Chevallet, M.
AU - Veronesi, G.
AU - Fuchs, A.
AU - Mintz, E.
AU - Michaud-Soret, I.
AU - Deniaud, A.
DB - Scopus
DO - 10.1016/j.bbagen.2016.12.012
IS - 6
KW - Copper
Labile metal nanoparticles
Metal homeostasis
Silver
X-ray fluorescence microscopy
Zinc
Animals
Biological Assay
Cell Biology
Cell Line
Homeostasis
Humans
Inflammation
Metal Nanoparticles
Nanotechnology
Oxidation-Reduction
Particle Size
Risk Assessment
Silver Compounds
Toxicity Tests
Zinc Oxide
metal nanoparticle
nanomaterial
silver nanoparticle
copper
cupric oxide
silver derivative
zinc oxide
controlled study
exocytosis
in vivo study
inflammation
nanomedicine
nanotechnology
priority journal
Review
synthesis
theranostic nanomedicine
trans Golgi network
animal
bioassay
cell line
chemically induced
chemistry
cytology
homeostasis
human
metabolism
oxidation reduction reaction
particle size
procedures
risk assessment
toxicity testing
M3 - Review
PY - 2017
SP - 1566-1577
ST - Impact of labile metal nanoparticles on cellular homeostasis. Current
developments in imaging, synthesis and applications
T2 - Biochimica et Biophysica Acta - General Subjects
TI - Impact of labile metal nanoparticles on cellular homeostasis. Current
developments in imaging, synthesis and applications
85009469576&doi=10.1016%2fj.bbagen.2016.12.012&partnerID=40&md5=b1a05186e84e3feda5c
663e54aa39d74
VL - 1861
ID - 5514
ER -
TY - JOUR
AB - Antibiotic era is coming to the end, becouse of the wrong selecting of
antibiotics and combination therapy and the mutation skills of bacteria. In recent
years, the use of nanosilver particles for therapeutic purposes has become more
relevant. These particles have an antibacterial effect and a fairly good effect on
local inflammatory processes. We made suspension of silver nanoparticles size 50 nm
by the physic method and introduced into laboratory rats. Our studies demonstrates
that argentium nano-particles can be administered in physical (non-chemical)
pathways. It does not have LD50, however, its consumption with high doses for
prolonged period of time leads to histomorphological changes in healthy tissues. ©
2019, Advanced Scientific Research. All rights reserved.
AU - Chichiveishvili, N.
AU - Tavkhelidze, T.
AU - Khubulava, S.
AU - Pitchkhaia, G.
AU - Kristesashvili, J.
AU - Kacadze, D.
DB - Scopus
IS - 1
KW - Drug toxicity.a
nanoparticle
silver nanoparticle
animal experiment
animal model
animal tissue
Article
body movement
chronic inflammation
chronic toxicity
controlled study
defecation
edema
emphysema
erythrocyte
feeding
histopathology
LD50
lethality
liver congestion
lung congestion
morphological trait
nonhuman
particle size
rat
M3 - Article
PY - 2019
SP - 713-715
ST - Study of LD50 and chronic toxicity of suspension of silver nanoparticles size
50 nm in laboratory rats
T2 - International Journal of Pharmaceutical Research
TI - Study of LD50 and chronic toxicity of suspension of silver nanoparticles size
50 nm in laboratory rats
85065082999&partnerID=40&md5=a3ede26ff901eece5e5c05fdc8af4762
VL - 11
ID - 5373
ER -
TY - JOUR
AB - Introduction: Perioperative anesthesia and analgesia exacerbate
immunosuppression in immunocompromised cancer patients. The natural killer (NK)
cell is a critical part of anti-tumor immunity. We compared the effects of two
different anesthesia and analgesia methods on the NK cell cytotoxicity (NKCC) in
patients undergoing breast cancer surgery. Methods: Fifty patients undergoing
breast cancer resection were randomly assigned to receive propofol-remifentanil
anesthesia with postoperative ketorolac analgesia (Propofol-ketorolac groups) or
sevoflurane-remifentanil anesthesia with postoperative fentanyl analgesia
(Sevoflurane-fentanyl group). The primary outcome was NKCC, which was measured
before and 24 h after surgery. Post-surgical pain scores and inflammatory responses
measured by white blood cell, neutrophil, and lymphocyte counts were assessed.
Cancer recurrence or metastasis was evaluated with ultrasound and whole body bone
scan every 6 months for 2 years after surgery. Results: The baseline NKCC (%) was
comparable between the two groups (P = 0.082). Compared with the baseline value,
NKCC (%) increased in the Propofol-ketorolac group [15.2 (3.2) to 20.1 (3.5), P =
0.048], whereas it decreased in the Sevoflurane-fentanyl group [19.5 (2.8) to 16.4
(1.9), P = 0.032]. The change of NKCC over time was significantly different between
the groups (P = 0.048). Pain scores during 48 h after surgery and post-surgical
inflammatory responses were comparable between the groups. One patient in the
Sevoflurane-fentanyl group had recurrence in the contralateral breast and no
metastasis was found in either group. Conclusions: Propofol anesthesia with
postoperative ketorolac analgesia demonstrated a favorable impact on immune
function by preserving NKCC compared with sevoflurane anesthesia and postoperative
fentanyl analgesia in patients undergoing breast cancer surgery.
AN - WOS:000408154900007
AU - Cho, J. S.
AU - Lee, M. H.
AU - Kim, S. I.
AU - Park, S.
AU - Park, H. S.
AU - Oh, E.
AU - Lee, J. H.
AU - Koo, B. N.
DO - 10.7150/ijms.20064
IS - 10
PY - 2017
SN - 1449-1907
SP - 970-976
ST - The Effects of Perioperative Anesthesia and Analgesia on Immune Function in
Patients Undergoing Breast Cancer Resection: A Prospective Randomized Study
T2 - INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
TI - The Effects of Perioperative Anesthesia and Analgesia on Immune Function in
Patients Undergoing Breast Cancer Resection: A Prospective Randomized Study
VL - 14
ID - 6683
ER -
TY - JOUR
AB - In Parkinson disease (PD), the dopaminergic (DAergic) neurons in the
substantia nigra undergo degeneration. While the exact mechanism for the
degeneration is still not completely understood, neuronal apoptosis and
inflammation are thought to play roles. We have recently obtained evidence that
matrix metalloproteinase (MMP)-3 plays a crucial role in the apoptotic signal in
DAergic cells as well as activation of microglia. The present study tested whether
doxycycline might modulate MMP-3 and provide neuroprotection of DAergic neurons.
Doxycycline effectively suppressed the expression of MMP-3 induced in response to
cellular stress in the DAergic CATH.a cells. This was accompanied by protection of
CATH.a cells as well as primary cultured mesencephalic DAergic neurons via
attenuation of apoptosis. The active form of MMP-3, released under the cell stress
condition, was also decreased in the presence of doxycycline. In addition,
doxycycline led to downregulation of MMP-3 in microglial BV-2 cells exposed to
lipopolysaccharide (LPS). This was accompanied by suppression of production of
nitric oxide and TNFa, as well as gene expression of iNOS, TNF-α, IL-1β, and COX-2.
In vivo, doxycycline provided neuroprotection of the nigral DAergic neurons
following MPTP treatment, as assessed by tyrosine hydroxylase immunocytochemistry
and silver staining, and suppressed microglial activation and astrogliosis as
assessed by Iba-1 and GFAP immunochemistry, respectively. Taken together,
doxycycline showed neuroprotective effect on DAergic system both in vitro and in
vivo and this appeared to derive from antiapoptotic and anti-inflammatory
mechanisms involving downregulation of MMP-3. © 2009 Springer Science+Business
Media, LLC.
AU - Cho, Y.
AU - Son, H. J.
AU - Kim, E. M.
AU - Choi, J. H.
AU - Kim, S. T.
AU - Ji, I. J.
AU - Choi, D. H.
AU - Joh, T. H.
AU - Kim, Y. S.
AU - Hwang, O.
DB - Scopus
DO - 10.1007/s12640-009-9078-1
IS - 4
KW - Dopaminergic neurons
Doxycycline
Matrix metalloproteinase-3
Microglia
Parkinson's disease
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Animals
Cell Death
Cells, Cultured
Dopamine
Enzyme-Linked Immunosorbent Assay
Female
Gene Expression Regulation, Enzymologic
Male
Matrix Metalloproteinase 3
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins
Neurons
Nitrites
Pregnancy
Rats
Silver Staining
Statistics, Nonparametric
Substantia Nigra
1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine
cell marker
cyclooxygenase 2
doxycycline
interleukin 1beta
lipopolysaccharide
nitric oxide
protein Iba 1
stromelysin
unclassified drug
dopamine
nerve protein
neuroprotective agent
nitrite
animal cell
animal experiment
animal model
apoptosis
article
astrocytosis
cell stress
controlled study
down regulation
drug mechanism
immunocytochemistry
in vivo study
male
microglia
mouse
nerve cell culture
neuroprotection
nonhuman
Parkinson disease
priority journal
protein expression
rat
silver staining
striatonigral degeneration
animal
C57BL mouse
cell culture
cell death
cytology
dose response
drug effect
female
genetics
metabolism
methodology
nerve cell
nick end labeling
nonparametric test
pregnancy
Sprague Dawley rat
substantia nigra
ultrastructure
M3 - Article
PY - 2009
SP - 361-371
ST - Doxycycline is neuroprotective against nigral dopaminergic degeneration by a
dual mechanism involving MMP-3
TI - Doxycycline is neuroprotective against nigral dopaminergic degeneration by a
dual mechanism involving MMP-3
74949110283&doi=10.1007%2fs12640-009-9078-
1&partnerID=40&md5=cfab74759b2139ec5fd6a5efbc09a0db
VL - 16
ID - 5835
ER -
TY - CPAPER
AB - Biocompatibility of photoluminescent silicon nanocrystals was tested using
standard cytotoxicity protocols with murine macrophage cell line RAW 264.7. We
investigated the cytotoxicity and inflammatory responses of cells exposed to
silicon nanocrystals by several biological endpoints. Cell death ratio,
morphological changes, and the levels of nitric oxide production were studied.
Spatial position of the nanoparticles relative to cell body was investigated using
fluorescent microscopy. No statistically significant cytotoxicity or inflammatory
response was detected with autoclaved silicon nanoparticles at concentrations up to
20 mu g/ml in RAW 264.7 cells. The present study of murine macrophages, exposed to
autoclaved silicon nanocrystals, will help define safety requirements for
comparable nanoparticle biomedical applications.
AN - WOS:000250804900038
AU - Choi, J. H.
AU - Zhang, Q.
AU - Hitchins, V. M.
AU - Wang, N. S.
AU - Reipa, V.
DO - 10.1117/12.734222
PY - 2007
T2 - NANOENGINEERING: FABRICATION, PROPERTIES, OPTICS, AND DEVICES IV
TI - Cytotoxicity of the photoluminescent silicon nanocrystals
VL - 6645
ID - 6144
ER -
TY - JOUR
AB - Photoluminescent silicon nanoparticles have a bright and stable fluorescence
and are promising candidates for bio-imaging, cell staining and drug delivery. With
increasing development of nanotechnology applications for biomedicine, an
understanding of the potential toxicity of nanoparticles is needed to assess safety
concerns for clinical applications. The objective of this study was to compare
biological responses of silicon nanoparticles (SNs, 3 nm diameter) with silicon
microparticles (SMs, similar to 100-3000 nm diameter) in cultured murine
macrophages (RAW 264.7) using standard protocols for assessing cytotoxicity/cell
viability and inflammatory responses developed for micron-sized particles. SNs and
SMs were exposed to macrophages with and without addition of endotoxin
lipopolysaccharide (LIPS), a positive inducer of tumor necrosis factor-alpha (TNF-
alpha), interleukin 6 (IL-6), and nitric oxide (NO). Cytotoxicity was assayed using
the dye exclusion and MTT assays. Cell supernatants were assayed for production
TNF-alpha, IL-6 and NO. SNs at concentrations <= 20 mu g ml(-1) exhibited no
cytotoxicity or inflammatory responses; however, SNs and SMs > 20 and 200 mu g ml(-
1), respectively, increased cytotoxicity compared with controls. SMs induced
concentration-related increases in TNF-alpha and IL-6 production; in contrast, the
production of these cytokines was shown to decrease with increasing concentrations
of SNs. NO production was not induced by SNs or SMs alone. Fluorescence microscopy
demonstrated that SNs were associated with the macrophages, either internalized or
attached to cell membranes. In conclusion, evaluating differences in biological
responses for nanoparticles compared with microparticles of the same material may
help improve tests to assess biological responses of nanoparticles that may be used
in biomedical applications. Copyright (C) 2008 John Wiley & Sons, Ltd.
AN - WOS:000262271000008
AU - Choi, J.
AU - Zhang, Q.
AU - Reipa, V.
AU - Wang, N. S.
AU - Stratmeyer, M. E.
AU - Goering, P. L.
DA - JAN
DO - 10.1002/jat.1382
IS - 1
PY - 2009
SN - 0260-437X
1099-1263
SP - 52-60
ST - Comparison of cytotoxic and inflammatory responses of photoluminescent
silicon nanoparticles with silicon micron-sized particles in RAW 264.7 macrophages
TI - Comparison of cytotoxic and inflammatory responses of photoluminescent
silicon nanoparticles with silicon micron-sized particles in RAW 264.7 macrophages
VL - 29
ID - 6354
ER -
TY - JOUR
AB - Nanoparticles (range under 100 nm) prepared by different technology modes
including physical, chemical, biological have many applications. Like in the same
way silver nanoparticles are used for different beneficial actions like
antimicrobial- antibacterial, antifungal and antiviral, anti-inflammatory,
anticancer, water treatment, cosmetics, and in the textiles industry. As silver
nanoparticles have shown wide application by different mechanisms against various
pathophyisiological conditions. To maintain safety under their use, the study of
the toxicity of silver nanoparticles has become more important. Health agencies
like WHO, NIOSH, EPA, EFSA & EU have issued guidelines for unrisky exposure limit
of silver nanopartricles in drinking water, food and breathing. The main purpose of
this article is to summarize genotoxicity, cytotoxicity, neurotoxicity,
reproductive toxicity of silver nanoparticles in both in vitro and in vivo studies
focused on mechanism and methods of detection. The main mechanism of silver
nanoparticles toxicity involves disruption of the mitochondrial respiratory chain,
which results in the generation of ROS and the stoppage of ATP synthesis which
further leads to a cascade of toxic events. ROS production measured by the
technique like flow cytometry using DCFHDA dye and other method includes a confocal
microscope, lipid peroxidation, etc. Different assay techniques used for evaluation
of different kind of toxicities such as the comet assay, MTT assay, and
histological assay, are also discussed. © 2022 Informa UK Limited, trading as
AU - Choudhary, A.
AU - Singh, S.
AU - Ravichandiran, V.
DB - Scopus
DO - 10.1080/15376516.2022.2064257
IS - 9
KW - cytotoxicity
genotoxicity
neurotoxicity
ROS
Antifungal Agents
Antiviral Agents
Drinking Water
Metal Nanoparticles
Silver
drinking water
silver
silver nanoparticle
antifungal agent
antiinfective agent
antivirus agent
metal nanoparticle
acrosome
breathing
cell line
cell membrane
comet assay
DNA damage
female genital system
flow cytometry
histopathology
in vitro study
in vivo study
lipid peroxidation
mitochondrion
MTT assay
practice guideline
respiratory chain
Review
sperm quality
synthesis
toxicity assay
M3 - Review
PY - 2022
SP - 650-661
ST - Toxicity, preparation methods and applications of silver nanoparticles: an
update
T2 - Toxicology Mechanisms and Methods
TI - Toxicity, preparation methods and applications of silver nanoparticles: an
update
85129616600&doi=10.1080%2f15376516.2022.2064257&partnerID=40&md5=8d9802527dd1e96d02
7e782a7ca01c19
VL - 32
ID - 5116
ER -
TY - JOUR
AB - Wounds associated with diabetes mellitus are the most severe co-morbidities,
which could be progressed to cause cell necrosis leading to amputation. Statistics
on the recent status of the diabetic wounds revealed that the disease affects 15%
of diabetic patients, where 20% of them undergo amputation of their limb.
Conventional therapies are found to be ineffective due to changes in the molecular
architecture of the injured area, urging novel deliveries for effective treatment.
Therefore, recent researches are on the development of new and effective wound care
materials. Literature is evident in providing potential tools in topical drug
delivery for wound healing under the umbrella of nanotechnology, where nano-
scaffolds and nanofibers have shown promising results. The nano-sized particles are
also known to promote healing of wounds by facilitating proper movement through the
healing phases. To date, focuses have been made on the efficacy of silver
nanoparticles (AgNPs) in treating the diabetic wound, where these nanoparticles are
known to exploit potential biological properties in producing anti-inflammatory and
antibacterial activities. AgNPs are also known to activate cellular mechanisms
towards the healing of chronic wounds; however, associated toxicities of AgNPs are
of great concern. This review is an attempt to illustrate the use of AgNPs in wound
healing to facilitate this delivery system in bringing into clinical applications
for a superior dressing and treatment over wounds and ulcers in diabetes patients.
© 2020
AU - Choudhury, H.
AU - Pandey, M.
AU - Lim, Y. Q.
AU - Low, C. Y.
AU - Lee, C. T.
AU - Marilyn, T. C. L.
AU - Loh, H. S.
AU - Lim, Y. P.
AU - Lee, C. F.
AU - Bhattamishra, S. K.
AU - Kesharwani, P.
AU - Gorain, B.
C7 - 110925
DB - Scopus
DO - 10.1016/j.msec.2020.110925
KW - Antibacterial property
Clinical aspects
Diabetic wound
Effective dressing
Nanotechnology
Silver nanoparticle
Bandages
Diabetic Foot
Humans
Metal Nanoparticles
Silver
Wound Healing
Artificial limbs
Cell death
Diseases
Metal nanoparticles
Particle size
antiinfective agent
metal nanoparticle
silver
Cellular mechanisms
Clinical application
Molecular architecture
Nano-sized particles
Topical drug deliveries
bandage
chemistry
diabetic foot
human
metabolism
pathology
wound healing
M3 - Review
PY - 2020
ST - Silver nanoparticles: Advanced and promising technology in diabetic wound
therapy
TI - Silver nanoparticles: Advanced and promising technology in diabetic wound
therapy
85083296998&doi=10.1016%2fj.msec.2020.110925&partnerID=40&md5=d14b2a2aeecd13dac87d8
e358fcee115
VL - 112
ID - 5272
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) find increasing applications, and therefore
humans and the environment are increasingly exposed to them. However, potential
toxicological implications are not sufficiently known. Here we investigate effects
of AgNPs (average size 120. nm) on zebrafish in vitro and in vivo, and compare them
to human hepatoma cells (Huh7). AgNPs are incorporated in zebrafish liver cells
(ZFL) and Huh7, and in zebrafish embryos. In ZFL cells AgNPs lead to induction of
reactive oxygen species (ROS), endoplasmatic reticulum (ER) stress response, and
TNF-α. Transcriptional alterations also occur in pro-apoptotic genes p53 and Bax.
The transcriptional profile differed in ZFL and Huh7 cells. In ZFL cells, the ER
stress marker BiP is induced, concomitant with the ER stress marker ATF-6 and
spliced XBP-1 after 6. h and 24. h exposure to 0.5. g/L and 0.05. g/L AgNPs,
respectively. This indicates the induction of different pathways of the ER stress
response. Moreover, AgNPs induce TNF-α. In zebrafish embryos exposed to 0.01, 0.1,
1 and 5. mg/L AgNPs hatching was affected and morphological defects occurred at
high concentrations. ER stress related gene transcripts BiP and Synv are
significantly up-regulated after 24. h at 0.1 and 5. mg/L AgNPs. Furthermore,
transcriptional alterations occurred in the pro-apoptotic genes Noxa and p21. The
ER stress response was strong in ZFL cells and occurred in zebrafish embryos as
well. Our data demonstrate for the first time that AgNPs lead to induction of ER
stress in zebrafish. The induction of ER stress can have several consequences
including the activation of apoptotic and inflammatory pathways. © 2013 Elsevier
Inc.
AU - Christen, V.
AU - Capelle, M.
AU - Fent, K.
DB - Scopus
DO - 10.1016/j.taap.2013.06.011
IS - 2
KW - ER stress response
Nanoparticles
Toxicity
Zebrafish
Animals
Cell Line, Tumor
Cell Survival
Embryo, Nonmammalian
Endoplasmic Reticulum Stress
Environmental Pollutants
Humans
Liver
Metal Nanoparticles
Particle Size
Real-Time Polymerase Chain Reaction
Silver
Species Specificity
Spectrophotometry, Atomic
Surface Properties
Transcription, Genetic
activating transcription factor 6
protein Bax
protein Noxa
protein p21
protein p53
silver nanoparticle
X box binding protein 1
animal cell
animal embryo
animal experiment
animal tissue
apoptosis
article
controlled study
cytotoxicity
embryo
endoplasmic reticulum stress
exposure
gene expression
gene targeting
hatching
hepatoma cell
human
human cell
in vitro study
in vivo study
liver cell
morphology
nonhuman
oxidative stress
tumor suppressor gene
upregulation
water analysis
zebra fish
M3 - Article
PY - 2013
SP - 519-528
ST - Silver nanoparticles induce endoplasmatic reticulum stress response in
zebrafish
TI - Silver nanoparticles induce endoplasmatic reticulum stress response in
zebrafish
84883758937&doi=10.1016%2fj.taap.2013.06.011&partnerID=40&md5=77b3f6940545abc13afb0
08e5c156b57
VL - 272
ID - 5587
ER -
TY - JOUR
AB - Gymnema sylvestre is a plant that is enriched in bioactive compounds. In
particular, gymnemic acids (GA) and phenolic compounds (PC) are pharmaceutically
important. There is a commercial demand for naturally occurring bioactive
compounds, but their availability is limited due to geographical and seasonal
variations. The elicitation approach can enhance the biosynthesis of phytochemicals
during in vitro culture of G. sylvestre. Here, to further improve gymnemic acid II
(GA II) and phenolic compounds (PC) production by G. sylvestre, cell suspension
cultures (CSC), which has attracted attention for the production of essential
phytochemicals, was explored using copper oxide nanoparticles (CuO NPs). Callus was
obtained on MS medium containing 2,4-dichlorophenoxyacetic acid, kinetin,
phytoagar, and sucrose. Agar-free MS medium was used to initiate CSC, which was
treated with three concentrations of CuO NPs (1, 3 or 5 mg/L). Treatment for 48 h
with 3 mg/L CuO NPs resulted in the greatest yields of GA II, total phenolics, and
flavonoids. The cultures also displayed pronounced antioxidant, antidiabetic, anti-
inflammatory, antibacterial, antifungal, and anticancer activities. The use of CuO
NPs (3 mg/L) significantly increased the production of GA II (nine-fold) and PC
compared to unamended CSC. We propose that CSC and use of nanoparticles (NPs) as a
new generation of elicitors, offer a suitable prospect for the production of
bioactive compounds.
AN - WOS:000473748100200
AU - Chung, I. M.
AU - Rajakumar, G.
AU - Subramanian, U.
AU - Venkidasamy, B.
AU - Thiruvengadam, M.
C7 - 2165
DA - MAY 2
DO - 10.3390/app9102165
IS - 10
PY - 2019
SN - 2076-3417
ST - Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds
Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br
T2 - APPLIED SCIENCES-BASEL
TI - Impact of Copper Oxide Nanoparticles on Enhancement of Bioactive Compounds
Using Cell Suspension Cultures of Gymnema sylvestre (Retz.) R. Br
VL - 9
ID - 6537
ER -
TY - JOUR
AB - The preparation of graphene-based nanomaterials (GBNs) with appropriate
stability and biocompatibility is crucial for their use in biomedical applications.
In this work, three GBNs differing in size and/or functionalization have been
synthetized and characterized, and their in vitro biological effects were compared.
Pegylated graphene oxide (GO-PEG, 200-500 nm) and flavin mononucleotide-stabilized
pristine graphene with two different sizes (PG-FMN, 200-400 nm and 100-200 nm) were
administered to macrophages, chosen as cellular model due to their key role in the
processing of foreign materials and the regulation of inflammatory responses. The
results showed that cellular uptake of GBNs was mainly influenced by their lateral
size, while the inflammatory potential depended also on the type of
functionalization. PG-FMN nanomaterials (both sizes) triggered significantly higher
nitric oxide (NO) release, together with some intracellular metabolic changes,
similar to those induced by the prototypical inflammatory stimulus LPS. NMR
metabolomics revealed that macrophages incubated with smaller PG-FMN displayed
increased levels of succinate, itaconate, phosphocholine and phosphocreatine,
together with decreased creatine content. The latter two variations were also
detected in cells incubated with larger PG-FMN nanosheets. On the other hand, GO-
PEG induced a decrease in the inflammatory metabolite succinate and a few other
changes distinct from those seen in LPS-stimulated macrophages. Assessment of TNF-
alpha secretion and macrophage surface markers (CD80 and CD206) further
corroborated the low inflammatory potential of GO-PEG. Overall, these findings
revealed distinct phenotypic and metabolic responses of macrophages to different
GBNs, which inform on their immunomodulatory activity and may contribute to guide
their therapeutic applications.
AN - WOS:000518493000027
AU - Cicuendez, M.
AU - Fernandes, M.
AU - Ayan-Varela, M.
AU - Oliveira, H.
AU - Feito, M. J.
AU - Diez-Orejas, R.
AU - Paredes, J. I.
AU - Villar-Rodil, S.
AU - Vila, M.
AU - Portoles, M. T.
AU - Duarte, I. F.
C7 - 110709
DA - FEB
DO - 10.1016/j.colsurfb.2019.110709
PY - 2020
SN - 0927-7765
1873-4367
ST - Macrophage inflammatory and metabolic responses to graphene-based
nanomaterials differing in size and functionalization
T2 - COLLOIDS AND SURFACES B-BIOINTERFACES
TI - Macrophage inflammatory and metabolic responses to graphene-based
nanomaterials differing in size and functionalization
VL - 186
ID - 6430
ER -
TY - JOUR
AB - Fucoidan is a polysaccharide built from L-fucose molecules. The main source
of this polysaccharide is the extracellular matrix of brown seaweed (Phaeophyta),
but it can be also isolated from invertebrates such as sea urchins (Echinoidea) and
sea cucumbers (Holothuroidea). Interest in fucoidan is related to its broad
biological activity, including possible antioxidant, anti-inflammatory, antifungal,
antiviral or antithrombotic effects. The potential application of fucoidan in the
pharmaceutical technology is also due to its ionic nature. The negative charge of
the molecule results from the presence of sulfate residues in the C-2 and C-4
positions, occasionally in C-3, allowing the formation of complexes with other
oppositely charged molecules. Fucoidan is non-toxic, biodegradable and
biocompatible compound approved by Food and Drug Administration (FDA) as Generally
Recognized As Safe (GRAS) category as food ingredient. Fucoidan plays an important
role in the pharmaceutical technology, so in this work aspects concerning its
pharmaceutical characteristics and designing of various dosage forms
(nanoparticles, liposomes, microparticles, and semisolid formulations) with
fucoidan itself and with its combinations with other polymers or components that
give a positive charge were reviewed. Advantages and limitations of fucoidan
utilization in the pharmaceutical technology were also discussed. © 2019 by the
authors.
AU - Citkowska, A.
AU - Szekalska, M.
AU - Winnicka, K.
C7 - 458
DB - Scopus
DO - 10.3390/md17080458
IS - 8
KW - Fucoidan
Fucospheres
Marine-derived
Multifunctional polymer
Pharmaceutical formulations
Polysaccharide
Animals
Chemistry, Pharmaceutical
Phaeophyta
Polysaccharides
Sea Cucumbers
Sea Urchins
Seaweed
Technology, Pharmaceutical
chitosan
cisplatin
curcumin
doxorubicin
fucoidin
heparin
liposome
methotrexate
nanoparticle
polymer
silver nitrate
tissue plasminogen activator
biomaterial
polysaccharide
anticoagulation
apoptosis
biocompatibility
cross linking
cytokine production
drug design
drug dosage form
drug formulation
drug isolation
drug mechanism
drug safety
drug structure
Food and Drug Administration
Fucus vesiculosus
genotoxicity
human
hydrogel
hydrophilicity
Laminaria japonica
nonhuman
particle size
pH
Review
temperature
Undaria pinnatifida
viscosity
animal
brown alga
chemistry
medicinal chemistry
pharmaceutics
procedures
sea cucumber
sea urchin
seaweed
M3 - Review
PY - 2019
ST - Possibilities of fucoidan utilization in the development of pharmaceutical
dosage forms
T2 - Marine Drugs
TI - Possibilities of fucoidan utilization in the development of pharmaceutical
dosage forms
85070219424&doi=10.3390%2fmd17080458&partnerID=40&md5=60f227ce03ad04366db853d519874
fff
VL - 17
ID - 5437
ER -
TY - JOUR
AB - Anti-inflammatory molecules often display little affinity for inflamed
tissues, leading to low accumulation into this site of action (and inefficiency),
and high incidence of severe side effects. To face the problem, numerous strategies
have been proposed, i.e., chemical modifications to the drug molecule, and
engineering of drug nanocarriers. The later approach to the problem can result in
optimized drug biodistribution and concentration into the target region, thus
enhancing the anti-inflammatory effect while reducing the associated drug toxicity.
Such nanoparticulate systems offer remarkable possibilities when they are made of
biodegradable polymers, lipid-based structures, and/or inorganic particles. Recent
advances in the field have been devoted to the optimization of the in vivo fate and
effectiveness of these drug nanocarriers, e.g., passive targeting strategies based
on the functionalization of nanoparticle surface with special biomolecules. In this
contribution, we analyze the possibilities and future perspectives of nanoparticle
therapy in inflammatory processes. © 2012 Bentham Science Publishers.
AU - Clares, B.
AU - Ruiz, M. A.
AU - Gallardo, V.
AU - Arias, J. L.
DB - Scopus
DO - 10.2174/092986712800784676
IS - 19
KW - Arthritis
Enhanced permeation and retention effect
Inflammation
Ligand-mediated drug/gene delivery
Nanoparticle
Passive drug targeting
Animals
Humans
Nanoparticles
celecoxib
dexamethasone
diclofenac
gold nanoparticle
ibuprofen
inorganic compound
lipid
liposome
lutetium 177
methylprednisolone
nanocarrier
nanomaterial
nanoparticle
niosome
palmitic acid
phosphatidylcholine
poloxamer
polymer
polysorbate 80
prednisolone
radioisotope
silver nanoparticle
stearic acid
triacylglycerol
tripalmitin
tristearin
unindexed drug
biocompatibility
biodegradation
chemical modification
drug accumulation
drug blood level
drug distribution
drug formulation
drug half life
drug release
drug safety
drug stability
emulsion
human
hydrophilicity
hydrophobicity
in vivo study
inflammation
lipid bilayer
lipogenesis
liposomal delivery
microemulsion
molecule
nonhuman
particle size
polymerization
rabbit
review
M3 - Review
PY - 2012
SP - 3203-3211
ST - Drug delivery to inflammation based on nanoparticles surface decorated with
biomolecules
T2 - Current Medicinal Chemistry
TI - Drug delivery to inflammation based on nanoparticles surface decorated with
biomolecules
84863682372&doi=10.2174%2f092986712800784676&partnerID=40&md5=91c6f2c60aa040fe3c072
0ef076c0b32
VL - 19
ID - 5670
ER -
TY - JOUR
AB - Large wounds are characterized by clinical and surgical challenges, requiring
numerous searches on demand for inexpensive biocompatible materials that produce
the best quality of cutaneous healing. Cellulose is a biopolymer of greatest
abundance, good biocompatibility and wide application due to its chemical and
physical properties. This study was aimed to develop and characterize low cost
membranes of wood cellulose nanofibrils for potential application as a wound
dressing in comparison to a commercial porous regenerating membrane. An inexpensive
mechanical method was used to defibrillate cellulose, and later the membranes were
obtained from the nanofibrils by filtering and drying under mild pressure. The
techniques employed for characterization included scanning electron microscopy,
physical testing, application in vivo and cost-effectiveness analysis. The
membranes presented promising physical properties for application as cutaneous
dressing, with characteristic translucency allowing the evaluation of the wound
without the need of removal and exchange of the dressing. Wood nanocellulose
dressing seems to be promising for wound care since it presented good adhesion to
the moist wound surfaces thus promoting the most efficient repitialization in the
first 4 days. No allergic reaction or inflammatory response to wood nanocellulose
dressings was observed. The wood nanocellulose membranes stand out as a potential
wound dressing, as it shows similar efficacy of bacterial cellulose dressing.
Besides the efficacy, the membrane developed in this study presents a simpler,
faster and cheaper manufacturing process, which may reduce the production cost by
99%.
AN - WOS:000524398000006
AU - Claro, F. C.
AU - Jordao, C.
AU - de Viveiros, B. M.
AU - Isaka, L. J. E.
AU - Villanova, J. A.
AU - Magalhaes, W. L. E.
C6 - APR 2020
DA - DEC
DO - 10.1007/s10570-020-03129-2
IS - 18
PY - 2020
SN - 0969-0239
1572-882X
SP - 10765-10779
ST - Low cost membrane of wood nanocellulose obtained by mechanical defibrillation
for potential applications as wound dressing
T2 - CELLULOSE
TI - Low cost membrane of wood nanocellulose obtained by mechanical defibrillation
for potential applications as wound dressing
VL - 27
ID - 6794
ER -
TY - JOUR
AB - BACKGROUND: In lung transplantation, ischemia-reperfusion injury associated
with mitochondrial damage can lead to graft rejection. Intact, exogenous
mitochondria provide a unique treatment option to salvage damaged cells within lung
tissue. METHODS: We developed a novel method to freeze and store allogeneic
mitochondria isolated from porcine heart tissue. Stored mitochondria were injected
into a model of induced ischemia-reperfusion injury using porcine ex-vivo lung
perfusion. Treatment benefits to immune modulation, antioxidant defense, and
cellular salvage were evaluated. These findings were corroborated in human lungs
undergoing ex-vivo lung perfusion. Lung tissue homogenate and primary lung
endothelial cells were then used to address underlying mechanisms. RESULTS:
Following cold ischemia, mitochondrial transplant reduced lung pulmonary vascular
resistance and tissue pro-inflammatory signaling and cytokine secretion. Further,
exogenous mitochondria reduced reactive oxygen species by-products and promoted
glutathione synthesis, thereby salvaging cell viability. These results were
confirmed in a human model of ex-vivo lung perfusion wherein transplanted
mitochondria decreased tissue oxidative and inflammatory signaling, improving lung
function. We demonstrate that transplanted mitochondria induce autophagy and
suggest that bolstered autophagy may act upstream of the anti-inflammatory and
antioxidant benefits. Importantly, chemical inhibitors of the MEK autophagy pathway
blunted the favorable effects of mitochondrial transplant. CONCLUSIONS: These data
provide direct evidence that mitochondrial transplant improves cellular health and
lung function when administered during ex-vivo lung perfusion and suggest the
mechanism of action may be through promotion of cellular autophagy. Data herein
contribute new insights into the therapeutic potential of mitochondrial transplant
to abate ischemia-reperfusion injury during lung transplant, and thus reduce graft
rejection.
AN - WOS:000989697300001
AU - Cloer, C. M.
AU - Givens, C. S.
AU - Buie, L. K.
AU - Rochelle, L. K.
AU - Lin, Y. T.
AU - Popa, S.
AU - Shelton, R. V. M.
AU - Zhan, J. M.
AU - Zimmerman, T. R.
AU - Jones, B. G.
AU - Lesesne, Z.
AU - Hogan, S. S.
AU - Petersen, T. H.
C6 - APR 2023
DA - MAY
DO - 10.1016/j.healun.2023.01.002
IS - 5
PY - 2023
SN - 1053-2498
1557-3117
ST - Mitochondrial transplant after ischemia reperfusion promotes cellular salvage
and improves lung function during ex-vivo lung perfusion
T2 - JOURNAL OF HEART AND LUNG TRANSPLANTATION
TI - Mitochondrial transplant after ischemia reperfusion promotes cellular salvage
and improves lung function during ex-vivo lung perfusion
VL - 42
ID - 6609
ER -
TY - JOUR
AB - In previous work, we developed novel antibacterial hybrid coatings based on
dextran containing dispersed Ag NPs (~5nm, DEX-Ag) aimed to offer dual protection
against two of the most common complications associated with implant surgery,
infections and rejection of the implant. However, their blood-material interactions
are unknown. In this study, we assess the hemocompatibility and biocompatibility of
DEX-Ag using fresh blood and two cell lines of the immune system, monocytes (THP-1
cells) and macrophages (PMA-stimulated THP-1 cells). Glass, polyurethane (PU) and
bare dextran (DEX) were used as reference surfaces. PU, DEX and DEX-Ag exhibited
non-hemolytic properties. Relative to glass (100%), platelet attachment on PU, DEX
and DEX-Ag was 15%, 10% and 34%, respectively. Further, we assessed cell morphology
and viability, pro-inflammatory cytokines expression (TNF-α and IL-1β), pro-
inflammatory eicosanoid expression (Prostaglandin E2, PGE2) and release of reactive
oxygen species (ROS, superoxide and H2O2) following incubation of the cells with
the surfaces. The morphology and cell viability of THP-1 cells were not affected by
DEX-Ag whereas DEX-Ag minimized spreading of PMA-stimulated THP-1 cells and caused
a reduction in cell viability (16% relative to other surfaces). Although DEX-Ag
slightly enhanced release of ROS, the expression of pro-inflammatory cytokines
remained minimal with similar levels of PGE2, as compared to the other surfaces
studied. These results highlight low toxicity of DEX-Ag and hold promise for future
applications in vivo. © 2013 Elsevier Inc.
AU - Coll Ferrer, M. C.
AU - Eckmann, U. N.
AU - Composto, R. J.
AU - Eckmann, D. M.
DB - Scopus
DO - 10.1016/j.taap.2013.07.023
IS - 3
KW - Cytocompatibility
Dextran
Hemocompatibility
Hybrid coating
Biomimetic Materials
Cell Survival
Cells, Cultured
Dextrans
Humans
Monocytes
Platelet Adhesiveness
Surface Properties
antibiotic agent
dextran
dextran silver
glass
hydrogen peroxide
interleukin 1beta
polyurethan
prostaglandin E2
superoxide
unclassified drug
article
biocompatibility
blood compatibility
cell structure
cell viability
controlled study
cytokine production
human
human cell
macrophage
monocyte
thrombocyte adhesion
M3 - Article
PY - 2013
SP - 703-712
ST - Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid
films
TI - Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid
films
84884724541&doi=10.1016%2fj.taap.2013.07.023&partnerID=40&md5=32b6d7be0ed514e323a3b
c25f0900aa2
VL - 272
ID - 5599
ER -
TY - JOUR
AB - Plastics are one of the most preoccupying emerging pollutants. Macroplastics
released into the environment degrade into microplastics and nanoplastics. Because
of their small size, these micro and nano plastic particles can enter the food
chain and, in addition to their ecotoxicological effects, contaminate humans with
still unknown biological effects. Plastics being particulate pollutants, they are
handled in the human body by scavenger cells such as macrophages, which are
important players in the immune system. In order to get a better appraisal of the
effects of plastic particles on macrophages, we have studied the effects of
unmodified polystyrene particles of 0.1, 1 and 10 micrometers of diameter, by a
combination of proteomics and targeted validation experiments. Proteomics showed
important adaptive changes of the proteome in response to exposure to plastics,
with more than one third of the detected proteins showing a significance change in
their abundance in response to cell exposure to at least one plastic bead size.
These changes affected for example mitochondrial, lysosomal or cytoskeletal
proteins. Although an increase in the mitochondrial transmembrane potential was
detected in response to 10 micrometer beads, no alteration in cell viability was
observed. Similarly, no lysosomal dysfunction and no alteration in the phagocytic
capability of the cells was observed in response to exposure to plastics. When the
inflammatory response was examined, no increase in the secretion of tumor necrosis
factor or interleukin 6 was observed. Oppositely, the secretion of these cytokines
in response to lipopolysaccharide was observed after exposure to plastics, which
suggested a decreased ability of macrophages to respond to bacterial infection. In
conclusion, these results provide a better understanding of the responses of
macrophages to exposure to polystyrene particles of different sizes.
AN - WOS:000820824900001
AU - Dalzon, B.
AU - Devcic, J.
AU - Diemer, H.
AU - Cianferani, S.
AU - Rabilloud, T.
C6 - JUL 2022
DA - AUG 11
DO - 10.1039/d2en00214k
IS - 8
PY - 2022
SN - 2051-8153
2051-8161
SP - 2827-2840
ST - Does size matter? A proteomics-informed comparison of the effects of
polystyrene beads of different sizes on macrophages
T2 - ENVIRONMENTAL SCIENCE-NANO
TI - Does size matter? A proteomics-informed comparison of the effects of
polystyrene beads of different sizes on macrophages
VL - 9
ID - 6823
ER -
TY - JOUR
AB - From a toxicological point of view, nanomaterials are of interest; because -
on account of their great surface area relative to mass -they tend to be more
reactive than the bulk chemicals from which they are derived. They might in some
cases have the potential to damage DNA directly, or could act via the induction of
oxidative stress. The comet assay (single cell gel electrophoresis) is widely used
to measure DNA strand breaks and also oxidised bases, by including in the procedure
digestion with lesion-specific enzymes such as formamidopyrimidine DNA glycosylase
(which converts oxidised purines to breaks) or endonuclease III (recognising
oxidised pyrimidines). We summarise reports in which these enzymes have been used
to study a variety of nanomaterials in diverse cell types. We also stress that it
is important to carry out tests of cell viability alongside the genotoxicity assay,
since cytotoxicity can lead to adventitious DNA damage. Different concentrations of
nanomaterials should be investigated, concentrating on a non-cytotoxic range; and
incubating for short and longer periods can give valuable information about the
mode of damage induction. The use of lesion-specific enzymes can substantially
enhance the sensitivity of the comet assay in detecting genotoxic effects.
AN - WOS:000402552600007
AU - Collins, A.
AU - El Yamani, N.
AU - Dusinska, M.
DA - JUN
DO - 10.1016/j.freeradbiomed.2017.02.001
PY - 2017
SN - 0891-5849
1873-4596
SP - 69-76
ST - Sensitive detection of DNA oxidation damage induced by nanomaterials
T2 - FREE RADICAL BIOLOGY AND MEDICINE
TI - Sensitive detection of DNA oxidation damage induced by nanomaterials
VL - 107
ID - 6789
ER -
TY - JOUR
AB - Cadaver grafts, laminated metallic materials, and synthetic fabrics have been
evaluated as dural substitutes. Use of cadaver tissues is limited by fear of
transmission of infectious disease while use of synthetic materials is associated
with implant encapsulation and foreign body reactions. The purpose of this study is
to evaluate the use of collagen film as a dural substitute. Collagen films prepared
from bovine skin were used to replace the dura of rabbits and histological
observations were made at 16, 28, 42, and 56 days postimplantation. Controls
consisted of dura that was removed and then reattached. Control dura showed no
signs of inflammation or adhesion to underlying tissue at 16 and 28 days
postimplantation. By 56 days postimplantation, extensive connective tissue
deposition was observed in close proximity to adjacent bone as well as pia
arachnoid adhesions. Implanted collagen film behaved in a similar manner to control
dura showing minimal inflammatory response at all time periods. At 56 days
postimplantation collagen film appeared strongly infiltrated by connective tissue
cells that deposited new collagen. The results of this study suggest that a
reconstituted type I collagen film crosslinked with cynanamide acts as a temporary
barrier preventing loss of fluid and adhesion formation. It is replaced after
approximately 2 months with host collagen with limited inflammatory and fibrotic
complications. Further studies are needed to completely characterize the new
connective tissue formed as well as long-term biocompatibility and functioning of a
reconstituted collagen dural substitute.
AN - WOS:A1991EV47200011
AU - Collins, R. L. L.
AU - Christiansen, D.
AU - Zazanis, G. A.
AU - Silver, F. H.
DA - FEB
DO - 10.1002/jbm.820250212
IS - 2
PY - 1991
SN - 0021-9304
SP - 267-276
ST - USE OF COLLAGEN FILM AS A DURAL SUBSTITUTE - PRELIMINARY ANIMAL STUDIES
T2 - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
TI - USE OF COLLAGEN FILM AS A DURAL SUBSTITUTE - PRELIMINARY ANIMAL STUDIES
VL - 25
ID - 6678
ER -
TY - JOUR
AB - Conventional treatment of inflammatory bowel disease (IBD) is based on the
daily administration of high doses of immune-suppressant or anti-inflammatory
drugs, often complicated by serious adverse effects. Thus, a carrier system that
delivers the drug specifically to the inflamed intestinal regions and shows
prolonged drug release would be desirable. The advent of TNF-α antibodies and other
biopharmaceuticals as potent and specific immune modulators in recent years has
broadened the treatment options in IBD, but further increases the necessity for
adequate drug delivery, as integrity and bioactivity of the biological active have
to be ensured. Exploiting the pathophysiological idiosyncrasies of IBD such as
increased mucus production, changes in the structure of the intestinal epithelium
and invasion of activated macrophages, different colloidal drug carrier systems
have been designed to passively or actively target the site of inflammation. This
review introduces different micro- or nanoparticulate drug delivery systems for
oral application in IBD therapy for the delivery of small molecular compounds and
next generation therapeutics from the group of biological (i.e. peptide and
nucleotide based) drugs. Crown Copyright © 2012 Published by Elsevier B.V. All
rights reserved.
AU - Collnot, E. M.
AU - Ali, H.
AU - Lehr, C. M.
DB - Scopus
DO - 10.1016/j.jconrel.2012.01.028
IS - 2
KW - Crohn's disease
Nanomedicine
Nanoparticle
Oral
Ulcerative colitis
Animals
Biological Agents
Drug Carriers
Humans
Inflammatory Bowel Diseases
Intestinal Mucosa
Models, Animal
Nanoparticles
Nucleotides
Polymers
Diseases
Drug delivery
Drug products
adalimumab
alicaforsen
budesonide
curcumin
cyclosporin
cyclosporin A
dexamethasone
drug carrier
eudragit
gelatin
glucocorticoid
infliximab
interleukin 10
Lactobacillus casei extract
liposome
mercaptopurine
mesalazine
methotrexate
microsphere
nanocarrier
nanocrystal
nanoparticle
olsalazine
rolipram
salazosulfapyridine
selenium
silver
tacrolimus
Activated macrophages
Adverse effect
Biopharmaceuticals
Carrier systems
Colloidal drug carriers
Conventional treatments
Drug carrier
Drug release
High dose
Immune modulators
Inflammatory bowel disease
Intestinal epithelium
Intestinal mucosa
Molecular compounds
Nanoparticulate drug delivery systems
Pathophysiological
bone marrow toxicity
chemical bond
Crohn disease
Cushing syndrome
dendritic cell
diabetes mellitus
diarrhea
disease model
disease severity
drug absorption
drug conjugation
drug contraindication
drug efficacy
drug formulation
drug stability
embryotoxicity
enteritis
enzyme degradation
experimental animal
gene expression
helper cell
hematologic disease
human
hydrophilicity
hypertension
immune deficiency
infection
intestine motility
intestine mucosa
kidney disease
lipid peroxidation
liver toxicity
lymphoma
macrophage
mast cell
membrane permeability
meta analysis (topic)
microemulsion
natural killer cell
nephrotoxicity
neutrophil
nonhuman
opportunistic infection
osteoporosis
oxidation
pancreatitis
paresthesia
particle size
priority journal
review
RNA interference
tremor
ulcerative colitis
Drug dosage
M3 - Review
PY - 2012
SP - 235-246
ST - Nano- and microparticulate drug carriers for targeting of the inflamed
intestinal mucosa
T2 - Journal of Controlled Release
TI - Nano- and microparticulate drug carriers for targeting of the inflamed
intestinal mucosa
84862644846&doi=10.1016%2fj.jconrel.2012.01.028&partnerID=40&md5=83427e10f92305efc9
ba9ed22872a8d4
VL - 161
ID - 5665
ER -
TY - JOUR
AB - The aim of this research was to evaluate the inflammatory and/or oxidative
damage related to silver nanoparticles (AgNPs), which are responsible for negative
effects on sperm physiology and metabolism. Thirty New Zealand White rabbit bucks
were divided into 5 experimental groups (6 animals/group): Control, treated with
0.9% NaCl; AgNP, treated with a 5 mM AgNP solution; LPS, treated with 50 g/kg b.w.
E. coli LPS; AgNPs + NSAID, treated with an anti-inflammatory drug at 0.2 mg/kg
b.w. and 5 mM AgNPs; and AgNPs + Vit E, treated with 0.18 mg/kg b.w. vitamin E and
5 mM AgNPs. Sperm quality and oxidative and inflammatory status were assessed at
different times (0-60 days). Two statistical models were built: the first evaluated
the effects of AgNPs and LPS (vs. Control), whereas the second evaluated the
protective effect of an NSAID and vitamin E against AgNP-induced damage. Three
principal component analyses were performed: sperm traits (motility, volume),
oxidative status (antioxidants, oxidative metabolites, and redox reactions), and
cytokines (TNF-α, IL-8, and IL-6). A negative effect on reproductive traits
resulted after NP administration. In particular, an inflammatory/oxidative response
took place in the reproductive tract during the first 2-3 wks of AgNP
administration (cytokine and oxidative metabolite generation); the
inflammatory/oxidative thrust impaired the status of rabbit tissues (seminal
plasma, sperm, and blood), inducing a response (increased antioxidant enzymes and
redox reactions) at 4-7 wks; oxidative stress, if not totally counteracted, likely
induced toxicity in the late phases of AgNP administration (8-9 wks). In
conclusion, exposure to silver nanoparticles produced a similar but more persistent
effect than that of LPS on rabbit reproductive tissues: AgNP administration
triggered a proinflammatory response linked to oxidative thrust, worsening many
sperm parameters. However, only anti-inflammatory treatment counteracted the
negative effects of AgNPs, whereas vitamin E seemed to act as an adjuvant,
attenuating the oxidative cascade. © 2020 Collodel Giulia et al.
AU - Collodel, G.
AU - Simona, M.
AU - Moretti, E.
AU - Cerretani, D.
AU - Lucia, M.
AU - Anna Ida, F.
AU - Laura, M.
AU - Gabriele, B.
AU - Cesare, C.
C7 - 6664062
DB - Scopus
DO - 10.1155/2020/6664062
KW - Animals
Antioxidants
Escherichia coli
Inflammation
Lipopolysaccharides
Male
Metal Nanoparticles
Oxidation-Reduction
Oxidative Stress
Oxygen
Principal Component Analysis
Rabbits
Semen
Silver
Vitamin E
alpha tocopherol
antioxidant
catalase
Escherichia coli lipopolysaccharide
interleukin 6
interleukin 8
lidocaine plus prilocaine
malonaldehyde
meloxicam
nitric oxide
silver nanoparticle
sodium chloride
vitalene e
lipopolysaccharide
metal nanoparticle
oxygen
silver
animal experiment
animal model
animal tissue
Article
blood
buck (mammal)
controlled study
enzyme activity
male
metabolite
New Zealand White (rabbit)
nonhuman
oxidative stress
semen parameters
seminal plasma
sperm function
sperm quality
spermatozoon density
spermatozoon motility
animal
chemistry
drug effect
inflammation
Leporidae
metabolism
principal component analysis
sperm
M3 - Article
PY - 2020
ST - Oxidative and/or Inflammatory Thrust Induced by Silver Nanoparticles in
Rabbits: Effect of Vitamin E or NSAID Administration on Semen Parameters
T2 - Mediators of Inflammation
TI - Oxidative and/or Inflammatory Thrust Induced by Silver Nanoparticles in
85099292234&doi=10.1155%2f2020%2f6664062&partnerID=40&md5=6a10ea059e8f5120b7cef09cc
9f58838
VL - 2020
ID - 5318
ER -
TY - JOUR
AB - Metallic nanomaterials, including silver, gold, and iron oxide, are being
utilized in an increasing number of fields and specialties. The use of nanosilver
as an antimicrobial agent is becoming ever-more common, whereas gold and Iron oxide
nanomaterials are frequently utilized in the medical field due to their recognized
"biocompatibility". Numerous reports have examined the general toxicity of these
nanomaterials; however, little data exists on how the introduction of these
nanomaterials, at nontoxic levels, affects normal cellular processes. In the
present study the impact of low levels of 10 nm silver (Ag-NP), gold (Au-NP), and
iron oxide nanoparticles (SPION) on epidermal growth factor (EGF) signal
transduction within the human epithelial cell line, A-431, was Investigated.
Following a biocompatibility assessment, the nanoparticle-induced interference at
four specific targets within the EGF signaling process was evaluated: (1)
nanoparticle-EGF association, (2) Akt and Erk phosphorylation, (3) Akt activity,
and (4) EGF-dependent gene regulation. For all tested nanoparticles, following
cellular exposure, a disruption in the EGF signaling response transpired; however,
the metallic composition determined the mechanism of alteration. In addition to
inducing high quantities of ROS, Ag-NPs attenuated levels of Akt and Erk
phosphorylation. Au-NPs were found to decrease EGF-dependent Akt and Erk
phosphorylation as well as inhibit Akt activity. Lastly, SPIONs produced a strong
alteration In EGF activated gene transcription, with targeted genes influencing
cell proliferation, migration, and receptor expression. These results demonstrate
that even at low doses, Introduction of Ag-NPs, Au-NPs, and SPIONs impaired the A-
431 cell line's response to EGF.
AN - WOS:000298316700076
AU - Comfort, K. K.
AU - Maurer, E. I.
AU - Hussain, S. M.
DA - DEC
DO - 10.1021/nn203785a
IS - 12
PY - 2011
SN - 1936-0851
1936-086X
SP - 10000-10008
ST - Interference of Silver, Gold, and Iron Oxide Nanoparticles on Epidermal
Growth Factor Signal Transduction in Epithelial Cells
T2 - ACS NANO
TI - Interference of Silver, Gold, and Iron Oxide Nanoparticles on Epidermal
Growth Factor Signal Transduction in Epithelial Cells
VL - 5
ID - 6250
ER -
TY - JOUR
AB - Local delivery of drugs or antimicrobial agents is a suitable approach in the
management of periodontitis when the infection is localized deep in the pockets and
does not adequately respond to mechanical debridement and/or systemic antibiotic
treatment. In this context, the objective of this study was to prepare new
biocomposite films with antimicrobial, anti-inflammatory, and good mechanical
properties to be applied in periodontal pockets. The composite film is eco-friendly
synthesized from poly(vinyl alcohol) (PVA) cross-linked with oxidized chitosan
(OxCS). Silver nanoparticles (AgNps) were inserted during film synthesis by adding
freshly chitosan-capped AgNps colloidal solution to the polymer mixture; the
addition of AgNps up to 1.44 wt.% improves the physico-chemical properties of the
film. The characterization of the films was performed by FT-IR, atomic mass
spectrometry, X-ray spectroscopy, and SEM. The films displayed a high swelling
ratio (162%), suitable strength (1.46 MPa), and excellent mucoadhesive properties
(0.6 N). Then, ibuprofen (IBF) was incorporated within the best film formulation,
and the IBF-loaded PVA/OxCS-Ag films could deliver the drug in a sustained manner
up to 72 h. The biocomposite films have good antimicrobial properties against
representative pathogens for oral cavities. Moreover, the films are biocompatible,
as demonstrated by in vitro tests on HDFa cell lines. © 2022 by the authors.
AU - Constantin, M.
AU - Lupei, M.
AU - Bucatariu, S. M.
AU - Pelin, I. M.
AU - Doroftei, F.
AU - Ichim, D. L.
AU - Daraba, O. M.
AU - Fundueanu, G.
C7 - 4
DB - Scopus
DO - 10.3390/polym15010004
IS - 1
KW - drug delivery systems
ibuprofen
periodontitis
poly(vinyl alcohol)/chitosan nanocomposite films
Biocompatibility
Chitosan
Crosslinking
Diseases
Mass spectrometry
Metal nanoparticles
Microorganisms
Nanocomposite films
Polyvinyl alcohols
Sols
Thin films
Biocomposite films
Drug-delivery systems
Ibuprofen
Oxidized chitosan
Periodontiti
Poly (vinyl alcohol) (PVA)
Poly(vinyl alcohol)
Poly(vinyl alcohol) (PVA)
Poly(vinyl alcohol)/chitosan nanocomposite film
Thin-films
M3 - Article
PY - 2023
ST - PVA/Chitosan Thin Films Containing Silver Nanoparticles and Ibuprofen for the
Treatment of Periodontal Disease
T2 - Polymers
TI - PVA/Chitosan Thin Films Containing Silver Nanoparticles and Ibuprofen for the
Treatment of Periodontal Disease
85146055699&doi=10.3390%2fpolym15010004&partnerID=40&md5=034794f9e2c2dab7e327d0337c
362f54
VL - 15
ID - 5008
ER -
TY - JOUR
AB - Alterations of skin homeostasis are widely diffused in our everyday life both
due to accidental injuries, such as wounds and burns, and physiological conditions,
such as late-stage diabetes, dermatitis, or psoriasis. These events are locally
characterized by an intense inflammatory response, a high generation of harmful
free radicals, or an impairment in the immune response regulation, which can
profoundly change the skin tissue’ repair process, vulnerability, and
functionality. Moreover, diabetes diffusion, antibiotic resistance, and abuse of
aggressive soaps and disinfectants following the COVID-19 emergency could be causes
for the future spreading of skin disorders. In the last years, hydroxycinnamic
acids and derivatives have been investigated and applied in several research fields
for their anti-oxidant, anti-inflammatory, and anti-bacterial activities. First, in
this study, we give an overview of these natural molecules’ current source and
applications. Afterwards, we review their potential role as valid alternatives to
the current therapies, supporting the management and rebalancing of skin disorders
and diseases at different levels. Also, we will introduce the recent advances in
the design of biomaterials loaded with these phenolic compounds, specifically
suitable for skin disorders treatments. Lastly, we will suggest future perspectives
for introducing hydroxycinnamic acids and derivatives in treating skin disorders. ©
2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Contardi, M.
AU - Lenzuni, M.
AU - Fiorentini, F.
AU - Summa, M.
AU - Bertorelli, R.
AU - Suarato, G.
AU - Athanassiou, A.
C7 - 999
DB - Scopus
IS - 7
KW - Anti-bacterial
Anti-inflammatory
Anti-oxidants
Biomaterials
Hydroxycinnamic acids
Skin care
Skin disorders
1 chloro 2,4 dinitrobenzene
anthocyanin
caffeic acid
chitosan
chlorogenic acid
cinnamic acid
corticosteroid
coumaric acid
essential oil
ferulic acid
flavonoid
gallic acid
hyaluronic acid
hydroxyproline
inflammasome
interleukin 13
interleukin 17
limonene
malonaldehyde
methotrexate
microsphere
molecular scaffold
monophenol monooxygenase
n,n dimethylformamide
nanocomposite
nitric oxide
phosphodiesterase IV inhibitor
plant extract
polyvinyl alcohol
quercetin
rosmarinic acid
silver nanoparticle
syringic acid
transforming growth factor beta
uric acid
vitamin D
Article
atopic dermatitis
biocompatibility
biodegradability
bioremediation
contact dermatitis
crystallization
DNA damage
drug formulation
electrochemistry
electrospinning
emotional stress
emulsion
epithelization
flow kinetics
gene therapy
genetic engineering
granulation tissue
human
hyperkeratosis
hyperpigmentation
immune response
inflammation
keratinocyte
Leishmania braziliensis
leishmaniasis
lipid peroxidation
melanogenesis
microalga
microbial community
microemulsion
oxidative stress
paw edema
phototherapy
prevalence
pruritus
psoriasis
rosemary
Saccharomyces cerevisiae
shear stress
signal transduction
skin defect
skin disease
skin irritation
skin permeability
skinfold thickness
thermogravimetry
thermostability
three dimensional printing
tissue regeneration
wound healing
M3 - Article
PY - 2021
ST - Hydroxycinnamic acids and derivatives formulations for skin damages and
disorders: A review
T2 - Pharmaceutics
TI - Hydroxycinnamic acids and derivatives formulations for skin damages and
disorders: A review
85110041221&doi=10.3390%2fpharmaceutics13070999&partnerID=40&md5=6ae54ab54575db7229
9785acc75d907e
VL - 13
ID - 5238
ER -
TY - JOUR
AB - Due to the increasing interest in nanotechnology in very large application
fields, including biotechnology, electronics and food industries, humans are
increasingly exposed to nanoparticles (NPs). Consequently, the question about the
safety of these nanomaterials and their impact on human health is a legitimate
concern. The liver is the primary organ of detoxification and is one of the tissues
that is most exposed to NPs. When they reach the bloodstream, NPs are mainly
internalized by liver cells. This review focuses on recent in vitro and in vivo
studies addressing the effects of organic and inorganic NPs on the liver.
Specifically, the impact of the NPs on hepatic enzyme activities, the inflammatory
response and genotoxicity processes will be described. Depending on the
physicochemical parameters of the NPs and the conditions of exposure, NPs could
lead to global liver injury.
AN - WOS:000509788200002
AU - Cornu, R.
AU - Beduneau, A.
AU - Martin, H.
C7 - 152344
DA - JAN
DO - 10.1016/j.tox.2019.152344
PY - 2020
SN - 0300-483X
ST - Influence of nanoparticles on liver tissue and hepatic functions: A review
T2 - TOXICOLOGY
TI - Influence of nanoparticles on liver tissue and hepatic functions: A review
VL - 430
ID - 6575
ER -
TY - JOUR
AB - The combination of sulfadiazine and pyrimethamine plus folinic acid is the
conventional treatment for congenital toxoplasmosis. However, this classical
treatment presents teratogenic effects and bone marrow suppression. In this sense,
new therapeutic strategies are necessary to reduce these effects and improve the
control of infection. In this context, biogenic silver nanoparticles (AgNp-Bio)
appear as a promising alternative since they have antimicrobial, antiviral, and
antiparasitic activity. The purpose of this study to investigate the action of
AgNp-Bio in BeWo cells, HTR-8/SVneo cells and villous explants and its effects
against Toxoplasma gondii infection. Both cells and villous explants were treated
with different concentrations of AgNp-Bio or combination of sulfadiazine +
pyrimethamine (SDZ + PYZ) in order to verify the viability. After, cells and villi
were infected and treated with AgNp-Bio or SDZ + PYZ in different concentrations to
ascertain the parasite proliferation and cytokine production profile. AgNp-Bio
treatment did not reduce the cell viability and villous explants. Significant
reduction was observed in parasite replication in both cells and villous explants
treated with silver nanoparticles and classical treatment. The AgNp-Bio treatment
increased of IL-4 and IL-10 by BeWo cells, while HTR8/SVneo cells produced
macrophage migration inhibitory factor (MIF) and IL-4. In the presence of T.
gondii, the treatment induced high levels of MIF production by BeWo cells and IL-6
by HTR8SV/neo. In villous explants, the AgNp-Bio treatment downregulated production
of IL-4, IL-6, and IL-8 after infection. In conclusion, AgNp-Bio can decrease T.
gondii infection in trophoblast cells and villous explants. Therefore, this
treatment demonstrated the ability to reduce the T. gondii proliferation with
induction of inflammatory mediators in the cells and independent of mediators in
chorionic villus which we consider the use of AgNp-Bio promising in the treatment
of toxoplasmosis in BeWo and HTR8/SVneo cell models and in chorionic villi. ©
Copyright © 2021 Costa, Ribeiro, Silva Franco, da Silva, de Araújo, Milián, Luz,
Guirelli, Nakazato, Mineo, Mineo, Barbosa and Ferro.
AU - Costa, I. N.
AU - Ribeiro, M.
AU - Silva Franco, P.
AU - da Silva, R. J.
AU - de Araújo, T. E.
AU - Milián, I. C. B.
AU - Luz, L. C.
AU - Guirelli, P. M.
AU - Nakazato, G.
AU - Mineo, J. R.
AU - Mineo, T. W. P.
AU - Barbosa, B. F.
AU - Ferro, E. A. V.
C7 - 623947
DB - Scopus
DO - 10.3389/fmicb.2020.623947
KW - congenital toxoplasmosis
nanoparticles treatment
placenta
Toxoplasma gondii
trophoblast
antiparasitic agent
biogenic silver nanoparticle
deoxycholate sodium
folinic acid
formazan
interleukin 10
interleukin 2
interleukin 4
interleukin 6
interleukin 8
macrolide
macrophage migration inhibition factor
migration inhibition factor
mitogen activated protein kinase 3
nanoparticle
nitric oxide
pyrimethamine
silver nanoparticle
spiramycin
sulfadiazine
unclassified drug
anemia
Article
BeWo cell line
blood vessel reactivity
CD4+ T lymphocyte
cell proliferation
cell viability
cesarean section
chorion villus
controlled study
cytokine production
cytotoxicity
cytotoxicity assay
cytotrophoblast
drug combination
enzyme activity
explant
fetus membrane
filamentous fungus
fungal biomass
gene expression
granulocytopenia
hippocampus
human
human cell
hypertension
immune response
Leishmania
limit of detection
macrophage
macrophage migration
megaloblastic anemia
MTT assay
nonhuman
parasite
parasitism
preeclampsia
protein expression
stillbirth
syncytiotrophoblast
tachyzoite
toxoplasmosis
Trypanosoma cruzi
M3 - Article
PY - 2021
ST - Biogenic Silver Nanoparticles Can Control Toxoplasma gondii Infection in Both
Human Trophoblast Cells and Villous Explants
T2 - Frontiers in Microbiology
TI - Biogenic Silver Nanoparticles Can Control Toxoplasma gondii Infection in Both
Human Trophoblast Cells and Villous Explants
85100542403&doi=10.3389%2ffmicb.2020.623947&partnerID=40&md5=7e2909d8d5c68ef5590fc0
be9415ed4c
VL - 11
ID - 5176
ER -
TY - JOUR
AB - Amaranthus spp. são plantas nefrotóxicas popularmente conhecidas como
"caruru". Em casos de intoxicação por estas plantas, a principal alteração
histopatológica está presente no rim, sob forma de nefrose tubular tóxica, porém em
alguns casos pode haver alterações cardíacas. Alterações no eletrocardiograma,
compatíveis com quadros de hipercalemia, foram descritas em suínos intoxicados por
Amaranthus retroflexus e lesões como degeneração e necrose de miócitos cardíacos
descritas em suínos intoxicados por A. caudatus e ovinos intoxicados por A.
spinosus. Há dúvidas com relação às alterações cardíacas, que, na maioria dos
casos, são incipientes, o que pode levar a erros de interpretação. Para a
realização do trabalho foram utilizados blocos parafinados oriundos de um surto
natural de intoxicação por A. spinosus no sudeste do Brasil. Esse estudo teve como
objetivo detectar a presença de alterações regressivas incipientes no miocárdio de
ovinos intoxicados por A. spinosus, através da utilização imuno-histoquímica do
anticorpo anti-troponina C. Foram utilizados fragmentos de coração de 8 ovinos
adultos e 2 fetos, intoxicados naturalmente por A. spinosus. Estes fragmentos foram
submetidos à técnica de imuno-histoquímica com a utilização do anticorpo anti-
troponina C. Pela avaliação imuno-histoquímica do coração dos oito ovinos adultos
observaram-se diversos grupos de miócitos com diminuição significativa ou ausência
de imunorreatividade para o anticorpo anti-troponina C; essas áreas correspondiam,
em grande parte, aos mesmos grupos de miócitos que apresentavam, pela coloração de
Hematoxilina e Eosina (H.E.) alterações que variavam de leve tumefação celular a
aumento da eosinofilia, perda de estriação, lise celular e cariólise, ou mais
raramente, acompanhadas de infiltrado inflamatório...
Amaranthus spp. are nephrotoxic plants popularly known as "pigweed". In cases of
poisoning by these plants, the main histopathological alteration is found in the
kidneys as toxic tubular nephrosis; however, in some cases, there may be cardiac
changes. ECG changes associated with hyperkalemia have been described in pigs
poisoned by Amaranthus retroflexus. Degeneration and necrosis of myocytes have been
described in pigs poisoned by A. caudatus and sheep poisoned by A. spinosus. There
are doubts regarding cardiac changes, since in most cases they are incipient and
don't exhibit inflammatory reaction, which can lead to misinterpretation. For this
study, paraffin blocks with tissues from a poisoning outbreak by A. spinosus in
southeastern Brazil were used. The objective of the study was to detect the
presence of incipient regressive changes in the myocardium of sheep poisoned by A.
spinosus using anti-troponin C antibody-based immunohistochemistry. Fragments of
hearts from 8 adult sheep and 2 fetuses naturally poisoned by A. spinosus were
used. In the immunohistochemistry evaluation of the 8 hearts from the adult sheep
there were several groups of myocytes with significant decrease or absence of
immunoreactivity for anti-troponin C antibody. In most cases, these same areas on
Hematoxylin and Eosin (HE) staining exhibited changes that varied from mild
cellular tumefaction to increased eosinophilia, as well as loss of striation, cell
lysis and karyolysis, sometimes accompanied by inflammatory infiltrate...
AD - Costa, Samay Z R
s.af
Peixoto, Paulo V
Universidade Federal Rural do Rio de Janeiro. Instituto de Zootecnia. Departamento
de Nutrição Animal e Pastagem. Seropédica. BR
Brust, Luiz Armando C
s.af
D'Avila, Mariana S
s.af
Santos, André M
s.af
Driemeier, David
Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Departamento
de Patologia Clínica Veterinária. Porto Alegre. BR
Nogueira, Vivian de A
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária.
Departamento de Epidemiologia e Saúde Pública. Seropédica. BR
França, Ticiana N
Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária.
Departamento de Epidemiologia e Saúde Pública. Seropédica. BR
AU - Costa, Samay Z. R.
AU - Peixoto, Paulo V.
AU - Brust, Luiz Armando C.
AU - D'Avila, Mariana S.
AU - Santos, André M.
AU - Driemeier, David
AU - Nogueira, Vivian de A.
AU - França, Ticiana N.
C1 - 20160403
DA - 2016/02
DB - LILACS
DO - 10.1590/S0100-736X2016000200004
IS - 2
KW - Amaranthaceae
Amaranthus spinosus
Caruru
Imuno-histoquímica
Insuficiência renal
Intoxicação por plantas
Miocárdio
Ovinos
Pigweed
Plant poisoning
Plantas tóxicas
Poisonous plants
Renal failure
Sheep, myocardium
Troponin C
Troponina C
LA - pt
PY - 2016
SN - 0100-736X
SP - 83-89
ST - Troponina C na detecção imuno-histoquímica de alterações regressivas precoces
no miocárdio de ovinos naturalmente intoxicados por Amaranthus spinosus
(Amaranthaceae)
T2 - Pesqui. vet. bras
TI - Troponina C na detecção imuno-histoquímica de alterações regressivas precoces
no miocárdio de ovinos naturalmente intoxicados por Amaranthus spinosus
(Amaranthaceae)
TT - Troponin C in the immunohistochemistry detection of early regressive changes
in sheep myocardium naturally poisoned by Amaranthus spinosus (Amaranthaceae)
736X2016000200083
VL - 36
ID - 4933
ER -
TY - JOUR
AB - Biodegradability and mechanical properties of magnesium alloys are attractive
for orthopaedic and cardiovascular applications. In order to study their
cytotoxicity usually bone cells are used. However, after implantation, diverse and
versatile cells are recruited and interact. Among the first ones coming into play
are cells of the immune system, which are responsible for the inflammatory
reaction. Macrophages play a central role in the inflammatory process due to the
production of cytokines involved in the tissue healing but also in the possible
failure of the implants. In order to evaluate the in vitro influence of the
degradation products of magnesium-based alloys on cytokine release, the extracts of
pure magnesium and two magnesium alloys (with gadolinium and silver as alloying
elements) were examined in an inflammatory in vitro model. Human promonocytic cells
(U937 cells) were differentiated into macrophages and further cultured with
magnesium-based extracts for 1 and 3 days (simulating early and late inflammatory
reaction phases), either at 37 °C or at 39 °C (mimicking normal and inflammatory
conditions, respectively). All extracts exhibit very good cytocompatibility on
differentiated macrophages. Results suggest that M1 and even more M2 profiles of
macrophage were stimulated by the extracts of Mg. Furthermore, Mg–10Gd and Mg–2Ag
extracts introduced a nuancing effect by rather inhibiting macrophage M1 profile.
Magnesium-based biomaterials could thus induce a faster inflammation resolution
while improving tissue repair. Statement of Significance: Macrophage are the key-
cells during inflammation and can influence the fate of tissue healing and implant
performance. Magnesium-based implants are biodegradable and bioactive. Here we
selected an in vitro system to model early and late inflammation and effect of
pyrexia (37 °C versus 39 °C). We showed the beneficial and nuancing effects of
magnesium (Mg) and the selected alloying elements (silver (Ag) and gadolinium (Gd))
on the macrophage polarisation. Mg extracts exacerbated simultaneously the
macrophage M1 and M2 profiles while Mg–2Ag and Mg–10Gd rather inhibited the M1
differentiation. Furthermore, 39 °C exhibited protective effect by either
decreasing cytokine production or promoting anti-inflammatory ones, with or without
extracts. Mg-based biomaterials could thus induce a faster inflammation resolution
while improving tissue repair. © 2019
AU - Costantino, M. D.
AU - Schuster, A.
AU - Helmholz, H.
AU - Meyer-Rachner, A.
AU - Willumeit-Römer, R.
AU - Luthringer-Feyerabend, B. J. C.
DB - Scopus
DO - 10.1016/j.actbio.2019.10.014
KW - Biomaterial
Cytocompatibility
Inflammation
Macrophage polarisation
Magnesium alloys
Alloying
Alloying elements
Biodegradability
Biomechanics
Cytology
Degradation
Macrophages
Pathology
Polarization
Repair
Silver alloys
Tissue
gadolinium
interleukin 1beta
magnesium
silver
Cell-be
Cell/B.E
Cell/BE
Cytokines
Gadolinia
In-vitro
Inflammatory reaction
Macrophage polarization
Tissue healing
Article
biocompatibility
biodegradability
biomechanics
cell viability
cytokine release
fever
human
human cell
inflammation
macrophage
tissue repair
U-937 cell line
wound healing
M3 - Article
PY - 2020
SP - 598-608
ST - Inflammatory response to magnesium-based biodegradable implant materials
T2 - Acta Biomaterialia
TI - Inflammatory response to magnesium-based biodegradable implant materials
85073820936&doi=10.1016%2fj.actbio.2019.10.014&partnerID=40&md5=0ba4feedca248e3dada
4a20869759316
VL - 101
ID - 5403
ER -
TY - JOUR
AB - Biodegradability and mechanical properties of magnesium alloys are attractive
for orthopaedic and cardiovascular applications. In order to study their
cytotoxicity usually bone cells are used. However, after implantation, diverse and
versatile cells are recruited and interact. Among the first ones coming into play
are cells of the immune system, which are responsible for the inflammatory
reaction. Macrophages play a central role in the inflammatory process due to the
production of cytokines involved in the tissue healing but also in the possible
failure of the implants. In order to evaluate the in vitro influence of the
degradation products of magnesium-based alloys on cytokine release, the extracts of
pure magnesium and two magnesium alloys (with gadolinium and silver as alloying
elements) were examined in an inflammatory in vitro model. Human promonocytic cells
(U937 cells) were differentiated into macrophages and further cultured with
magnesium-based extracts for 1 and 3 days (simulating early and late inflammatory
reaction phases), either at 37 degrees C or at 39 degrees C (mimicking normal and
inflammatory conditions, respectively). All extracts exhibit very good
cytocompatibility on differentiated macrophages. Results suggest that M1 and even
more M2 profiles of macrophage were stimulated by the extracts of Mg. Furthermore,
Mg-10Gd and Mg-2Ag extracts introduced a nuancing effect by rather inhibiting
macrophage M1 profile. Magnesium based biomaterials could thus induce a faster
inflammation resolution while improving tissue repair. Statement of Significance
Macrophage are the key-cells during inflammation and can influence the fate of
tissue healing and implant performance. Magnesium-based implants are biodegradable
and bioactive. Here we selected an in vitro system to model early and late
inflammation and effect of pyrexia (37 degrees C versus 39 degrees C). We showed
the beneficial and nuancing effects of magnesium (Mg) and the selected alloying
elements (silver (Ag) and gadolinium (Gd)) on the macrophage polarisation. Mg
extracts exacerbated simultaneously the macrophage M1 and M2 profiles while Mg-2Ag
and Mg-10Gd rather inhibited the M1 differentiation. Furthermore, 39 degrees C
exhibited protective effect by either decreasing cytokine production or promoting
anti-inflammatory ones, with or without extracts. Mg-based biomaterials could thus
induce a faster inflammation resolution while improving tissue repair. (C) 2019
Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
AN - WOS:000504504300048
AU - Costantino, M. D.
AU - Schuster, A.
AU - Helmholz, H.
AU - Meyer-Rachner, A.
AU - Willumeit-Romer, R.
AU - Luthringer-Feyerabend, B. J. C.
DA - JAN 1
DO - 10.1016/j.actbio.2019.10.014
PY - 2020
SN - 1742-7061
1878-7568
SP - 598-608
ST - Inflammatory response to magnesium-based biodegradable implant materials
T2 - ACTA BIOMATERIALIA
TI - Inflammatory response to magnesium-based biodegradable implant materials
VL - 101
ID - 6332
ER -
TY - JOUR
AB - Background: Damage or exposure of the dental pulp requires immediate
therapeutic intervention. Methods: This study assessed the biocompatibility of a
silver-containing PLGA/TCP-nanofabric scaffold (PLGA/Ag-TCP) in two in vitro
models, i.e. the material adapted on pre-cultured cells and cells directly cultured
on the material, respectively. Collagen saffolds with and without hyaluronan acid
(Coll-HA; Coll) using both cell culturing methods and cells growing on culture
plates served as reference. Cell viability and proliferation were assessed after
24, 48, and 72 h based on formazan formation and BrdU incorporation. Scaffolds were
harvested. Gene expression of interleukin(IL)-6, tumor necrosis factor (TNF)-alpha,
and alkaline phosphatase (AP) was assessed 24 h after stimulation. Results: In both
models formazan formation and BrdU incorporation was reduced by PLGA/Ag-TCP on
dental pulp cells, while no significant reduction was found in cells with Coll and
Coll-HA. Cells with PLGA/Ag-TCP for 72 h showed similar relative BrdU incorporation
than cells stimulated with Coll and Coll-HA. A prominent increase in the pro-
inflammatory genes IL-6 and TNF-α was observed when cells were cultured with
PLGA/Ag-TCP compared to the other groups. This increase was parallel with a slight
increase in AP expression. Overall, no differences between the two culture methods
were observed. Conclusions: PLGA/Ag-TCP decreased viability and proliferation rate
of human dental pulp cells and increased the pro-inflammatory capacity and alkaline
phosphatase expression. Whether these cellular responses observed in vitro
translate into pulp regeneration in vivo will be assessed in further studies. ©
2017 The Author(s).
AU - Cvikl, B.
AU - Hess, S. C.
AU - Miron, R. J.
AU - Agis, H.
AU - Bosshardt, D.
AU - Attin, T.
AU - Schmidlin, P. R.
AU - Lussi, A.
C7 - 57
DB - Scopus
DO - 10.1186/s12903-017-0348-7
IS - 1
KW - Capping
Dental pulp
In vitro techniques
Regeneration
Alkaline Phosphatase
Cell Proliferation
Cells, Cultured
Chlorine
Collagen
Dental Pulp
Drug Combinations
Humans
Hyaluronic Acid
In Vitro Techniques
Iodine
Lactic Acid
Materials Testing
Nanofibers
Polyglycolic Acid
Pulp Capping and Pulpectomy Agents
Salicylates
Silver
antiinfective agent
biomaterial
biomedical and dental materials
chlorine
collagen
hyaluronic acid
iodine
lactic acid
nanofiber
polyglycolic acid
polylactic acid-polyglycolic acid copolymer
salicylic acid derivative
silver
TCP (antiseptic)
cell culture
cell proliferation
comparative study
cytology
drug combination
drug effect
human
in vitro study
materials testing
metabolism
physiology
regeneration
tooth pulp
M3 - Article
PY - 2017
ST - Response of human dental pulp cells to a silver-containing PLGA/TCP-
nanofabric as a potential antibacterial regenerative pulp-capping material
T2 - BMC Oral Health
TI - Response of human dental pulp cells to a silver-containing PLGA/TCP-
nanofabric as a potential antibacterial regenerative pulp-capping material
85015199159&doi=10.1186%2fs12903-017-0348-
7&partnerID=40&md5=878339fff3d3a2653f895f12855e5b81
VL - 17
ID - 5503
ER -
TY - JOUR
AB - Human triple-negative breast cancer (TNBC) being an aggressive cancer type
accounts for about 15–20% of global breast cancer cases. In the present study, the
cytotoxicity of pure silver (AgVI) and silver/zinc oxide (Ag/ZnOVI) nanostructures
was evaluated against the TNBC cells. The nanostructures synthesized from a green
route using Vateria indica (L.) fruit extract were characterized to scrutinize
their formation, crystal phase, size, shape, and surface properties via FTIR, PXRD,
FE-SEM coupled with EDS spectroscopy, and BET analysis. The results of the studies
have unveiled the formation of 26.43 nm and 20.97 nm sized AgVI and Ag/ZnOVI
nanostructures in their purest form. The in-vitro anticancer study performed on
human TNBC cells [MDA-MB468] revealed the enhancement in the antiproliferative
potentiality of bimetallic Ag/ZnOVI nanostructures from 66.99 ± 0.13 to 79.73 ±
0.23 in comparison to pure AgVI nanostructures. In addition to this, the greenish
yellow-fluorescence observed in the TNBC nuclei during the AO-EB staining study
manifested the early apoptosis. Furthermore, the anti-inflammatory and cytotoxicity
study performed on the human RBC and normal NIH3T3 murine fibroblasts cells proved
the biocompatibility and non-toxic nature of the synthesized nanostructures with
membrane stabilization percentage up to 94.5 ± 0.001. Additionally, the antioxidant
and antidiabetic studies carried out have corroborated the radical scavenging and
α-amylase inhibition capability up to 85.87 ± 0.001 and 89.60 ± 0.002 %
respectively. Thus the overall results of the study substantiate the superlative
antioxidant, antidiabetic, and antiproliferative property of green synthesized AgVI
and Ag/ZnOVI nanostructures with excellent biocompatibility. © 2022 Elsevier B.V.
AU - D'Souza, J. N.
AU - Nagaraja, G. K.
AU - Prabhu, A.
AU - Navada, K. M.
AU - Kouser, S.
AU - Manasa, D. J.
C7 - 121450
DB - Scopus
DO - 10.1016/j.ijpharm.2022.121450
KW - Ag/ZnOVI nanostructures
Antidiabetic
Antiproliferative
Membrane stabilization
Vateria indica
Animals
Antioxidants
Dipterocarpaceae
Humans
Hypoglycemic Agents
Metal Nanoparticles
Mice
Nanostructures
NIH 3T3 Cells
Plant Extracts
Triple Negative Breast Neoplasms
nanomaterial
plant extract
silver
unclassified drug
Vateria indica extract
zinc oxide
antidiabetic agent
antioxidant
metal nanoparticle
adsorption
animal cell
Article
biocompatibility
Brunauer Emmett Teller method
controlled study
crystal
cytotoxicity
desorption
drug efficacy
drug structure
drug synthesis
human
human cell
in vitro study
isotherm
medicinal plant
membrane stabilization
NIH 3T3 cell line
nonhuman
particle size
pore size distribution
pore volume
porosity
surface area
surface property
X ray powder diffraction
animal
mouse
M3 - Article
PY - 2022
ST - AgVI and Ag/ZnOVI nanostructures from Vateria indica (L.) exert antioxidant,
antidiabetic, anti-inflammatory and cytotoxic efficacy on triple negative breast
cancer cells in vitro
TI - AgVI and Ag/ZnOVI nanostructures from Vateria indica (L.) exert antioxidant,
antidiabetic, anti-inflammatory and cytotoxic efficacy on triple negative breast
cancer cells in vitro
85122978468&doi=10.1016%2fj.ijpharm.2022.121450&partnerID=40&md5=f101471277943987a1
1637217ebfc1df
VL - 615
ID - 5109
ER -
TY - JOUR
AB - Background: Poly-lactic acid nanoparticles (PLA-NP) are a type of polymeric
NP, frequently used as nanomedicines, which have advantages over metallic NP such
as the ability to maintain therapeutic drug levels for sustained periods of time.
Despite PLA-NP being considered biocompatible, data concerning alterations in
cellular physiology are scarce. Methods: We conducted an extensive evaluation of
PLA-NP biocompatibility in human lung epithelial A549 cells using high throughput
screening and more complex methodologies. These included measurements of
cytotoxicity, cell viability, immunomodulatory potential, and effects upon the
cells' proteome. We used non-and green-fluorescent PLA-NP with 63 and 66 nm
diameters, respectively. Cells were exposed with concentrations of 2, 20, 100 and
200 mu g/mL, for 24, 48 and 72 h, in most experiments. Moreover, possible endocytic
mechanisms of internalization of PLA-NP were investigated, such as those involving
caveolae, lipid rafts, macropinocytosis and clathrin-coated pits. Results: Cell
viability and proliferation were not altered in response to PLA-NP. Multiplex
analysis of secreted mediators revealed a low-level reduction of IL-12p70 and
vascular epidermal growth factor (VEGF) in response to PLA-NP, while all other
mediators assessed were unaffected. However, changes to the cells' proteome were
observed in response to PLA-NP, and, additionally, the cellular stress marker
miR155 was found to reduce. In dual exposures of staurosporine (STS) with PLA-NP,
PLA-NP enhanced susceptibility to STS-induced cell death. Finally, PLA-NP were
rapidly internalized in association with clathrin-coated pits, and, to a lesser
extent, with lipid rafts. Conclusions: These data demonstrate that PLA-NP are
internalized and, in general, tolerated by A549 cells, with no cytotoxicity and no
secretion of pro-inflammatory mediators. However, PLA-NP exposure may induce
modification of biological functions of A549 cells, which should be considered when
designing drug delivery systems. Moreover, the pathways of PLA-NP internalization
we detected could contribute to the improvement of selective uptake strategies.
AN - WOS:000395576900002
AU - da Luz, C. M.
AU - Boyles, M. S. P.
AU - Falagan-Lotsch, P.
AU - Pereira, M. R.
AU - Tutumi, H. R.
AU - Santos, E. D.
AU - Martins, N. B.
AU - Himly, M.
AU - Sommer, A.
AU - Foissner, I.
AU - Duschl, A.
AU - Granjeiro, J. M.
AU - Leite, P. E. C.
C7 - 11
DA - JAN 31
DO - 10.1186/s12951-016-0238-1
PY - 2017
SN - 1477-3155
ST - Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications
in lung epithelial cells and are internalized by clathrin-coated pits and lipid
rafts
T2 - JOURNAL OF NANOBIOTECHNOLOGY
TI - Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications
in lung epithelial cells and are internalized by clathrin-coated pits and lipid
rafts
VL - 15
ID - 6536
ER -
TY - JOUR
AB - This research aimed to assess the influence of dietary addition of rutin on
inflammation, apoptosis and antioxidative responses in muscle of silver catfish
(Rhamdia quelen) challenged with Aeromonas hydrophila (A. hydrophila). Fish were
split into four groups as follows: control, 0.15% rutin, A. hydrophila, 0.15% rutin
+ A. hydrophila. After 2 weeks of feeding with standard or rutin diets, fish were
challenged or not with A. hydrophila for 1 week. Rutin-added diet abrogates A.
hydrophila induced-hemorrhage and inflammatory infiltration. It decreases A.
hydrophila induced-apoptosis through decreasing the ratio of Bax to Bcl-2 and
increasing phospho-Akt to Akt ratio. It diminishes the A. hydrophila induced-rise
in nitric oxide and superoxide anion levels and reestablishes superoxide dismutase
activity as well. Although such diet is unable to recover the levels of reduced
glutathione (GSH), cysteine and glutamate cysteine ligase, which are depleted as a
result of A. hydrophila infection, it diminishes the oxidized glutathione (GSSG)
content, thus decreasing GSSG to GSH ratio. It increases the levels of cysteine
residues of proteins and diminishes those of thiol-protein mixed disulfides, which
were changed after A. hydrophila challenge. Finally, it reduces A. hydrophila
induced-lipid peroxidation, markedly elevates ascorbic acid and thus reestablishes
total antioxidant capacity, whose levels were decreased after A. hydrophila
challenge. In conclusion, the dietary addition of rutin at 0.15% impairs A.
hydrophila-induced inflammatory response, inhibits A. hydrophila-induced apoptosis
and promotes cell survival. It also reduces the A. hydrophila-induced oxidative
stress and stimulates the antioxidative responses in muscle of A. hydrophila-
infected silver catfish. © 2019
AU - da Rosa, V. M.
AU - Ariotti, K.
AU - Bressan, C. A.
AU - da Silva, E. G.
AU - Dallaporta, M.
AU - Júnior, G. B.
AU - da Costa, S. T.
AU - de Vargas, A. C.
AU - Finamor, I. A.
AU - Pavanato, M. A.
C7 - 108611
DB - Scopus
DO - 10.1016/j.cbpc.2019.108611
KW - Cell survival
Diet
Fish
Infection
Oxidative stress
Aeromonas hydrophila
Animal Feed
Animals
Antioxidants
Apoptosis
Catfishes
Dietary Supplements
Fish Diseases
Gram-Negative Bacterial Infections
Muscles
Oxidative Stress
Protective Agents
Rutin
cysteine
glutamate cysteine ligase
glutathione
glutathione disulfide
nitric oxide
rutoside
superoxide
antioxidant
protective agent
animal experiment
animal model
apoptosis
Article
bacterial infection
catfish
donkey
inflammation
lipid peroxidation
muscle
muscle action potential
nonhuman
oxidative stress
priority journal
Rhamdia quelen
animal
animal food
dietary supplement
fish disease
Gram negative infection
immunology
metabolism
veterinary medicine
M3 - Article
PY - 2019
ST - Dietary addition of rutin impairs inflammatory response and protects muscle
of silver catfish (Rhamdia quelen) from apoptosis and oxidative stress in Aeromonas
hydrophila-induced infection
T2 - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
TI - Dietary addition of rutin impairs inflammatory response and protects muscle
85071473977&doi=10.1016%2fj.cbpc.2019.108611&partnerID=40&md5=2765794ff285d55aa6d0d
4912c11a47e
VL - 226
ID - 5385
ER -
TY - JOUR
AB - This research aimed to assess the influence of dietary addition of rutin on
inflammation, apoptosis and antioxidative responses in muscle of silver catfish
(Rhamdia quelen) challenged with Aeromonas hydrophila (A. hydrophila). Fish were
split into four groups as follows: control, 0.15% rutin, A. hydrophila, 0.15% rutin
+ A. hydrophila. After 2 weeks of feeding with standard or rutin diets, fish were
challenged or not with A. hydrophila for 1 week. Rutin-added diet abrogates A.
hydrophila induced-hemorrhage and inflammatory infiltration. It decreases A.
hydrophila induced-apoptosis through decreasing the ratio of Bax to Bcl-2 and
increasing phospho-Akt to Akt ratio. It diminishes the A. hydrophila induced-rise
in nitric oxide and superoxide anion levels and re-establishes superoxide dismutase
activity as well. Although such diet is unable to recover the levels of reduced
glutathione (GSH), cysteine and glutamate cysteine ligase, which are depleted as a
result of A. hydrophila infection, it diminishes the oxidized glutathione (GSSG)
content, thus decreasing GSSG to GSH ratio. It increases the levels of cysteine
residues of proteins and diminishes those of thiol-protein mixed disulfides, which
were changed after A. hydrophila challenge. Finally, it reduces A. hydrophila
induced-lipid peroxidation, markedly elevates ascorbic acid and thus reestablishes
total antioxidant capacity, whose levels were decreased after A. hydrophila
challenge. In conclusion, the dietary addition of rutin at 0.15% impairs A.
hydrophila-induced inflammatory response, inhibits A. hydrophila-induced apoptosis
and promotes cell survival. It also reduces the A. hydrophila-induced oxidative
stress and stimulates the antioxidative responses in muscle of A. hydrophila-
infected silver catfish.
AN - WOS:000496603700010
AU - da Rosa, V. M.
AU - Ariotti, K.
AU - Bressan, C. A.
AU - da Silva, E. G.
AU - Dallaporta, M.
AU - Junior, G. B.
AU - da Costa, S. T.
AU - de Vargas, A. C.
AU - Finamor, I. A.
C7 - 108611
DA - DEC
DO - 10.1016/j.cbpc.2019.108611
PY - 2019
SN - 1532-0456
1878-1659
ST - Dietary addition of rutin impairs inflammatory response and protects muscle
T2 - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
TI - Dietary addition of rutin impairs inflammatory response and protects muscle
VL - 226
ID - 6277
ER -
TY - JOUR
AB - Stryphnodendron adstringens (Martius) Coville is a medicinal plant described
as having pharmacological properties as anti-inflammatory and antimicrobial
activities. Silver chloride nanoparticles (AgCl-NPs) have shown great potential for
biomedical applications with efficient antimicrobial properties. Here, we report
the photosynthesis of AgCl-NPs using plant extract from S. adstringens (SaAgCl-NPs)
and their cytotoxic and antimicrobial activities. We photosynthesized SaAgCl-NPs
nearly spherical with low heterogeneity in size and enveloped by an organic
material layer responsible for colloidal stability. SaAgCl-NPs was non-cytotoxic
against mammalian VERO cells; however, SaAgCl-NPs presented remarkable antifungal
activity against the pathogenic yeast Cryptococcus neoformans (MIC80 of 0.32 µg/mL)
the causative agent of human cryptococcosis. Notable antibacterial activity was
observed against Gram-negative bacteria Pseudomonas aeruginosa (MIC80 of
2.56 µg/mL) e Serratia marcescens (MIC80 of 20.48 µg/mL) both microorganisms
associated with a variety of human infections, in particular pneumonia. In
contrast, Gram-positive bacteria Staphylococcus aureus and Staphylococcus
epidermidis, microorganisms that cause pathologies as skin infections, were less
susceptible to the SaAgCl-NPs both with MIC80 of 40.93 µg/mL. Thus, SaAgCl-NPs
represents an organic–inorganic hybrid nanomaterial with very low cytotoxicity
against mammalian cells and high antimicrobial efficiency against pathogenic
microorganisms and may be explored as an alternative to antimicrobial drugs. ©
2021, The Author(s), under exclusive licence to Springer Science+Business Media,
LLC part of Springer Nature.
AU - da S. Fernandes, D. G.
AU - Andrade, V. B.
AU - Lucena, L. N.
AU - Ambrosio, F. N.
AU - de Souza, A. L. M.
AU - Batista, B. L.
AU - Rolim, W. R.
AU - Seabra, A. B.
AU - Lombello, C. B.
AU - da Silva, F. D.
AU - Garcia, W.
DB - Scopus
DO - 10.1007/s10876-021-02011-w
IS - 2
Pathogenic microorganisms
Silver chloride nanoparticles
Stryphnodendron adstringens
M3 - Article
PY - 2022
SP - 687-695
ST - Cytotoxicity and Antimicrobial Properties of Photosynthesized Silver Chloride
Nanoparticles Using Plant Extract from Stryphnodendron adstringens (Martius)
Coville
TI - Cytotoxicity and Antimicrobial Properties of Photosynthesized Silver Chloride
Coville
85100540258&doi=10.1007%2fs10876-021-02011-
w&partnerID=40&md5=d8171cbe5db8a8871a814e739768237c
VL - 33
ID - 5145
ER -
TY - JOUR
AB - A morphologic study of the lungs was carried out in Swiss mice infected with
yeast isolated from Paracoccidioides brasiliensis (Pbl8). The lung was processed 1,
2, 4, and 8 weeks after inoculation for histologic staining with hematoxylin and
eosin (H&E), methenamine silver nitrate (Gomori-Grocott), and picrosirius to
qualitative and quantitative analyses of the granulomas and the presence of fungal
lesions. The numbers of CFUs/g counted in the lungs were 189.8 ± 20.64, 353.6 ±
46.21, 547.2 ± 108.1, and 295.2 ±89.17 in the first, second, fourth, and eighth
weeks, respectively. One week after infection, inflammatory cells and reticular and
collagens fibers, the latest typical of fibrosis, were detected. After 2 and 4
weeks, a progressive intensification of the infection and fibrosis was observed,
but in week 8 a more orga-nized granuloma was evident, with macrophages,
epithelioid cells, and yeasts in the central portion, and intense peripheral
basophilia. Pycnotic structures typical of apoptotic bodies were observed in weeks
1 and 8. The different histologic stain-ing used acted as a fundamental tool for
the study of the morphologic organization of granuloma formation. Copyright © 2009
by The American Society of Tropical Medicine and Hygiene.
AU - Da Silva, F. C.
AU - Svidzinski, T. I. E.
AU - Patussi, E. V.
AU - Cardoso, C. P.
AU - De Oliveira Dalalio, M. M.
AU - Hernandes, L.
DB - Scopus
DO - 10.4269/ajtmh.2009.80.798
IS - 5
KW - Animals
Granuloma
Lung
Lung Diseases, Fungal
Male
Mice
Mice, Inbred BALB C
Paracoccidioides
Paracoccidioidomycosis
Mus
Paracoccidioides brasiliensis
collagen fiber
eosin
hematoxylin
silver nitrate
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
basophilia
colony forming unit
controlled study
disease course
disease duration
epithelioid cell
fungus isolation
histopathology
inflammatory cell
inoculation
lung
lung fibrosis
lung granuloma
macrophage
male
micromorphology
mouse
nonhuman
qualitative analysis
reticulum cell
South American blastomycosis
Swiss Webster mouse
yeast
animal
Bagg albino mouse
granuloma
lung mycosis
microbiology
pathology
M3 - Article
PY - 2009
SP - 798-804
ST - Morphologic organization of pulmonary granulomas in mice infected with
T2 - American Journal of Tropical Medicine and Hygiene
TI - Morphologic organization of pulmonary granulomas in mice infected with
66149098517&doi=10.4269%2fajtmh.2009.80.798&partnerID=40&md5=956dd328be58b4acd34e6b
b3b201a1a2
VL - 80
ID - 5820
ER -
TY - JOUR
AB - In the present study, new-layered inorganic/organic hybrid of silver/talc
nanocomposites (Ag/Tlc-NPs) and its chitosan-capped derivative (Ag/Tlc/Csn NCs)
were biochemically synthesized utilizing Lawsonia inermis L. extract. The silver
nanoparticles (Ag NPs) were synthesized employing green method on the exterior
surface layer of talc mineral as a solid substrate. The negatively charged surface
layer of talc might function as templates and can attract the chitosan cations from
a solution to yield a layered hybrid structure, whose inorganic phase is formed by
Si-O-Ag bonds. Our results revealed that Ag NPs were formed on the exterior surface
of talc with a diameter with size of 124–215 nm. In addition, cytotoxicity, in
vitro antibacterial activity, and clinical effects of wound-healing ointments
containing talc were investigated. The results implied the successful synthesis of
Ag/Tlc/Csn NCs using the extract. The structures were safe up to 0.50 mg/mL. In
vitro studies confirmed antioxidant and antibacterial properties of Ag/Tlc/Csn NCs.
In sum, our findings showed that the ointments improve wound healing process by
inducing an anti-inflammatory M2 phenotype and bFGF, CD206, collagen1A, and IL-10
production that causes fibroblast migration and wound closure through influencing
M2 macrophage. Ag/Tlc/Csn is suggested to be taken into consideration as a medical
combination for improving infected wound healing and as a promising agent for
clinical administration. © 2021 Elsevier B.V.
AU - Daghian, S. G.
AU - Farahpour, M. R.
AU - Jafarirad, S.
C7 - 112294
DB - Scopus
DO - 10.1016/j.msec.2021.112294
KW - Chitosan
Collagen biosynthesis
Infected wound
Nanocomposites
Silver
Talc
Acceleration
Lawsonia Plant
Metal Nanoparticles
Static Electricity
Wound Healing
Bacteria
Cell culture
Silicon compounds
Silver compounds
antiinfective agent
chitosan
metal nanoparticle
nanocomposite
silver
talc
Ag$++$
Electrostatic attractions
Infected wounds
Inorganic-organic hybrid
Lawsonia inermis
Synthesised
Wound healing
acceleration
Lawsonia (plant)
static electricity
wound healing
Biochemistry
M3 - Article
PY - 2021
ST - Biological fabrication and electrostatic attractions of new layered
silver/talc nanocomposite using Lawsonia inermis L. and its chitosan-capped
inorganic/organic hybrid: Investigation on acceleration of Staphylococcus aureus
and Pseudomonas aeruginosa infected wound healing
TI - Biological fabrication and electrostatic attractions of new layered
silver/talc nanocomposite using Lawsonia inermis L. and its chitosan-capped
inorganic/organic hybrid: Investigation on acceleration of Staphylococcus aureus
and Pseudomonas aeruginosa infected wound healing
85109214091&doi=10.1016%2fj.msec.2021.112294&partnerID=40&md5=0d096770290281577dfd6
f4eac572568
VL - 128
ID - 5237
ER -
TY - JOUR
AB - Implantation of a drug-eluting stent is the most common treatment method for
patients with cardiovascular atherosclerosis. However, this treatment may delay re-
endothelialization, and the drug polymer-coated stent may induce thrombosis months
after a stent implantation. The development of polymer-free drug-eluting stents is
a promising approach to overcome these shortcomings. Titanium dioxide nanotubes
(TiO2-NTs) are excellent drug carriers and have been considered as a potential
material for polymer-free drug-eluting stents. However, TiO2-NTs reportedly induce
severe blood clotting, which is a significant shortcoming for use as a stent.
Vascular stents must be compatible with blood and must have antibacterial, anti-
inflammatory, and selective inhibitory activities in the abnormal hyperplasia of
smooth muscle cells, instead of delaying the re-endothelialization of endothelial
cells. To meet these requirements, we presented a composite material that featured
ultraviolet (UV) irradiation of TiO2-NTs-containing silver nanoparticles (AgNPs).
The AgNPs were loaded in the lumen of TiO2-NTs as a representative compound to
suppress the inflammatory response and hyperplasia. UV irradiation was performed as
a novel method to improve the anticoagulant ability of the AgNP-loaded TiO2-NTs.
The chemical state and biocompatibility of the UV-TiO2-NTs@AgNPs were evaluated. UV
irradiation strongly improved the anticoagulant ability of the TiO2-NTs and
moderated the release of Ag+ from AgNPs, which selectively suppressed the
inflammatory response and hyperplasia. Furthermore, the UV-TiO2-NTs@AgNPs-2
displayed enhanced biocompatibility evidenced by the inhibition of platelet
adhesion, bactericidal activity, selective suppression of the smooth muscle cell
proliferation, and inhibition of the adhesion of macrophages. The collective
findings indicate the potential of the photofunctionalized TiO2-NTs loaded with
AgNPs as a material for polymer-free drug-eluting stents.
AN - WOS:000526319100020
AU - Dai, S.
AU - Jiang, L.
AU - Liu, L. Y.
AU - Chen, J.
AU - Liao, Y. Z.
AU - He, S.
AU - Cui, J. W.
AU - Liu, X. Q.
AU - Zhao, A. S.
AU - Yang, P.
AU - Huang, N.
DA - APR
DO - 10.1021/acsbiomaterials.0c00041
IS - 4
PY - 2020
SN - 2373-9878
SP - 2038-2049
ST - Photofunctionalized and Drug-Loaded TiO2 Nanotubes with Improved Vascular
Biocompatibility as a Potential Material for Polymer-Free Drug-Eluting Stents
T2 - ACS BIOMATERIALS SCIENCE & ENGINEERING
TI - Photofunctionalized and Drug-Loaded TiO2 Nanotubes with Improved Vascular
VL - 6
ID - 5976
ER -
TY - JOUR
AB - Implantation of a drug-eluting stent is the most common treatment method for
patients with cardiovascular atherosclerosis. However, this treatment may delay re-
endothelialization, and the drug polymer-coated stent may induce thrombosis months
after a stent implantation. The development of polymer-free drug-eluting stents is
a promising approach to overcome these shortcomings. Titanium dioxide nanotubes
(TiO2-NTs) are excellent drug carriers and have been considered as a potential
material for polymer-free drug-eluting stents. However, TiO2-NTs reportedly induce
severe blood clotting, which is a significant shortcoming for use as a stent.
Vascular stents must be compatible with blood and must have antibacterial, anti-
inflammatory, and selective inhibitory activities in the abnormal hyperplasia of
smooth muscle cells, instead of delaying the re-endothelialization of endothelial
cells. To meet these requirements, we presented a composite material that featured
ultraviolet (UV) irradiation of TiO2-NTs-containing silver nanoparticles (AgNPs).
The AgNPs were loaded in the lumen of TiO2-NTs as a representative compound to
suppress the inflammatory response and hyperplasia. UV irradiation was performed as
a novel method to improve the anticoagulant ability of the AgNP-loaded TiO2-NTs.
The chemical state and biocompatibility of the UV-TiO2-NTs@AgNPs were evaluated. UV
irradiation strongly improved the anticoagulant ability of the TiO2-NTs and
moderated the release of Ag+ from AgNPs, which selectively suppressed the
inflammatory response and hyperplasia. Furthermore, the UV-TiO2-NTs@AgNPs-2
displayed enhanced biocompatibility evidenced by the inhibition of platelet
adhesion, bactericidal activity, selective suppression of the smooth muscle cell
proliferation, and inhibition of the adhesion of macrophages. The collective
findings indicate the potential of the photofunctionalized TiO2-NTs loaded with
AgNPs as a material for polymer-free drug-eluting stents. © 2020 American Chemical
Society.
AU - Dai, S.
AU - Jiang, L.
AU - Liu, L.
AU - Chen, J.
AU - Liao, Y.
AU - He, S.
AU - Cui, J.
AU - Liu, X.
AU - Zhao, A.
AU - Yang, P.
AU - Huang, N.
DB - Scopus
IS - 4
antibacterial
anticoagulant
silver nanoparticles (AgNPs)
titanium dioxide nanotubes (TiO2-NTs)
UV treatment
Drug-Eluting Stents
Humans
Metal Nanoparticles
Nanotubes
Pharmaceutical Preparations
Polymers
Silver
Titanium
Adhesion
Artificial organs
Biocompatibility
Blood
Cell proliferation
Diseases
Drug delivery
Drug products
Endothelial cells
Irradiation
Muscle
Silver alloys
Stents
Titanium dioxide
silver nanoparticle
drug
metal nanoparticle
nanotube
polymer
silver
titanium
titanium dioxide
Anti-inflammatories
Bactericidal activity
Representative compound
Smooth muscle cell proliferation
Titanium dioxide nanotubes
Ultraviolet irradiations
animal cell
animal tissue
anticoagulation
Article
bactericidal activity
biocompatibility
cell proliferation
drug release
drug selectivity
histocompatibility
human
human cell
hyperplasia
in vivo study
male
nonhuman
priority journal
rat
smooth muscle cell
ultraviolet irradiation
drug eluting stent
TiO2 nanoparticles
M3 - Article
PY - 2020
SP - 2038-2049
ST - Photofunctionalized and Drug-Loaded TiO2 Nanotubes with Improved Vascular
T2 - ACS Biomaterials Science and Engineering
TI - Photofunctionalized and Drug-Loaded TiO2 Nanotubes with Improved Vascular
85083916466&doi=10.1021%2facsbiomaterials.0c00041&partnerID=40&md5=3478b8af440f18df
00a70e7d820a1545
VL - 6
ID - 5322
ER -
TY - JOUR
AB - Multidrug resistance of the pathogenic microorganisms to the antimicrobial
drugs has become a major impediment toward successful diagnosis and management of
infectious diseases. Recent advancements in nanotechnology-based medicines have
opened new horizons for combating multidrug resistance in microorganisms. In
particular, the use of silver nanoparticles (AgNPs) as a potent antibacterial agent
has received much attention. The most critical physico-chemical parameters that
affect the antimicrobial potential of AgNPs include size, shape, surface charge,
concentration and colloidal state. AgNPs exhibits their antimicrobial potential
through multifaceted mechanisms. AgNPs adhesion to microbial cells, penetration
inside the cells, ROS and free radical generation, and modulation of microbial
signal transduction pathways have been recognized as the most prominent modes of
antimicrobial action. On the other side, AgNPs exposure to human cells induces
cytotoxicity, genotoxicity, and inflammatory response in human cells in a cell-type
dependent manner. This has raised concerns regarding use of AgNPs in therapeutics
and drug delivery. We have summarized the emerging endeavors that address current
challenges in relation to safe use of AgNPs in therapeutics and drug delivery
platforms. Based on research done so far, we believe that AgNPs can be engineered
so as to increase their efficacy, stability, specificity, biosafety and
biocompatibility. In this regard, three perspectives research directions have been
suggested that include (1) synthesizing AgNPs with controlled physico-chemical
properties, (2) examining microbial development of resistance toward AgNPs, and (3)
ascertaining the susceptibility of cytoxicity, genotoxicity, and inflammatory
response to human cells upon AgNPs exposure. © 2016 Dakal, Kumar, Majumdar and
Yadav.
AU - Dakal, T. C.
AU - Kumar, A.
AU - Majumdar, R. S.
AU - Yadav, V.
C7 - 1831
DB - Scopus
DO - 10.3389/fmicb.2016.01831
IS - NOV
Cytotoxicity
Genotoxicity
Multidrug resistance
Physico-chemical property
8 hydroxydeoxyguanosine
ampicillin
bacterium lipopolysaccharide
catalase
chloramphenicol
flavoprotein
glutathione
heme oxygenase 1
hydrogen peroxide
hydroxyl radical
hypochlorous acid
malonaldehyde
metallothionein
mitogen activated protein kinase kinase 4
potassium ion
reduced nicotinamide adenine dinucleotide phosphate oxidase
sigma factor
silver nanoparticle
single stranded DNA binding protein
superoxide
transcription factor Nrf2
unindexed drug
uridine diphosphate glucose dehydrogenase
analytical parameters
apoptosis
Article
binding affinity
biocompatibility
biosafety
colloidal state
cytotoxicity
DNA damage
drug efficacy
drug specificity
drug stability
gene expression
genotoxicity
Hepatitis B virus
human
Human immunodeficiency virus
inflammation
lipid peroxidation
nonhuman
oxidative stress
particle size
physical chemistry
signal transduction
surface charge
surface property
unfolded protein response
M3 - Article
PY - 2016
ST - Mechanistic basis of antimicrobial actions of silver nanoparticles
TI - Mechanistic basis of antimicrobial actions of silver nanoparticles
85006802503&doi=10.3389%2ffmicb.2016.01831&partnerID=40&md5=16362d8533614e620271601
fc44d2c5e
VL - 7
ID - 5550
ER -
TY - JOUR
AB - Copper is an essential metal for life, but is toxic at high concentrations.
In mammalian cells, two copper transporters are known, CTR1 and CTR2. In order to
gain insights on the possible influence of the import pathway on cellular responses
to copper, two copper challenges were compared: one with copper ion, which is
likely to use preferentially CTR1, and one with a copper-polyacrylate complex,
which will be internalized via the endosomal pathway and is likely to use
preferentially CTR2. A model system consisting in the J774A1 mouse macrophage
system, with a strong endosomal/lysosomal pathway, was used. In order to gain wide
insights into the cellular responses to copper, a proteomic approach was used. The
proteomic results were validated by targeted experiments, and showed differential
effects of the import mode on cellular physiology parameters. While the
mitochondrial transmembrane potential was kept constant, a depletion in the free
glutahione content was observed with copper (ion and polylacrylate complex). Both
copper-polyacrylate and polyacrylate induced perturbations in the cytoskeleton and
in phagocytosis. Inflammatory responses were also differently altered by copper ion
and copper-polyacrylate. Copper-polyacrylate also perturbed several metabolic
enzymes. Lastly, enzymes were used as a test set to assess the predictive value of
proteomics. Significance: Proteomic profiling provides an in depth analysis of the
alterations induced on cells by copper under two different exposure modes to this
metal, namely as the free ion or as a complex with polyacrylate. The cellular
responses were substantially different between the two exposure modes, although
some cellular effects are shared, such as the depletion in free glutathione.
Targeted experiments were used to confirm the proteomic results. Some metabolic
enzymes showed altered activities after exposure to the copper-polyacrylate
complex. The basal inflammatory responses were different for copper ion and for the
copper-polyacrylate complex, while the two forms of copper inhibited
lipopolysaccharide-induced inflammatory responses.
AN - WOS:000637802000004
AU - Dalzon, B.
AU - Devcic, J.
AU - Bons, J.
AU - Torres, A.
AU - Diemer, H.
AU - Ravanel, S.
AU - Cianferani, S.
AU - Carapito, C.
AU - Rabilloud, T.
C6 - MAR 2021
C7 - 104178
DA - MAY 15
DO - 10.1016/j.jprot.2021.104178
PY - 2021
SN - 1874-3919
1876-7737
ST - A proteomic view of cellular responses of macrophages to copper when added as
ion or as copper-polyacrylate complex
T2 - JOURNAL OF PROTEOMICS
TI - A proteomic view of cellular responses of macrophages to copper when added as
ion or as copper-polyacrylate complex
VL - 239
ID - 6815
ER -
TY - JOUR
AB - Immunotoxicology sensu lato comprises not only toxicity toward immune cells,
but also biological reactions from immune cells exposed to toxicants, reactions
that may have deleterious effects at the organismal level. Within this wide frame,
a specific case of interest is represented by the response of macrophages to
particulate materials, with the epitome examples of asbestos and crystalline
silica. For such toxicants that are both persistent and often encountered in an
occupational setting, i.e. at low but repeated doses, there is a need for in vitro
systems that can take into account these two parameters. Currently, most in vitro
systems are used in an acute exposure mode, i.e., with a single dose and a readout
made shortly if not immediately after exposure. We describe here how adequate
changes of the culture methods applied to the murine macrophage cell line J774A.1
enable longer periods of culture (several days), which represents a first
opportunity to address the persistence and dose-rate issues. To respond to this,
the protocol uses a reduction in the concentration of the animal serum used for
cell culture, as well as a switch from fetal to adult serum, which is less rich in
proliferation factors. By doing so, we have considerably reduced cell
proliferation, which is a problem with cell lines when they are supposed to
represent slowly-dividing cells such as resident macrophages. We also succeeded in
maintaining the differentiated functions of macrophages, such as phagocytosis or
inflammatory responses, over the whole culture period. Furthermore, the presence of
serum, even at low concentrations, provides excellent cell viability and keeps the
presence of a protein corona on particulate materials, a feature that is known to
strongly modulate their effects on cells and is lost in serum-free culture. Besides
data showing the impact of these conditions on macrophages cell line cultures,
illustrative examples are shown on silica- and cobalt-based pigments.
AN - WOS:001002896400001
AU - Dalzon, B.
AU - Torres, A.
AU - Devcic, J.
AU - Fenel, D.
AU - Sergent, J. A.
AU - Rabilloud, T.
C7 - 780778
DA - DEC 6
DO - 10.3389/ftox.2021.780778
PY - 2021
SN - 2673-3080
ST - A Low-Serum Culture System for Prolonged in Vitro Toxicology Experiments on a
Macrophage System
T2 - FRONTIERS IN TOXICOLOGY
TI - A Low-Serum Culture System for Prolonged in Vitro Toxicology Experiments on a
Macrophage System
VL - 3
ID - 6772
ER -
TY - JOUR
AB - Introduction Leishmania braziliensis infection induces a large spectrum of
lesions that clinically manifest as nodules or papules that progress to ulcers.
Although it is already known that T helper cells predominate in the lesions,
cytotoxic T cells have also been reported to be present, and their role in
leishmaniasis immunopathogenesis is not well known. This study investigated the
amounts of CD8+ and granzyme B+ cells in different clinical forms of human
cutaneous leishmaniasis (CL). Methods Forty tissue fragments from early (E-CL) and
late CL (L-CL) lesions and from disseminated leishmaniasis (DL) - papules and
ulcers - were characterized. The inflamed area per fragment was calculated, and the
CD8 and granzyme B expression levels in the infiltrates were quantified by counting
positive cells in 15 fields. The localization of CD8 and granzyme B was graded
subjectively. Results Inflammation was higher in L-CL and DL ulcers. CD8 expression
was increased in late ulcerated lesions compared to recent lesions. The increase in
CD8+ cells also correlated with the duration of the lesion. Papules had a higher
frequency of granzyme B+ cells than E-CL lesions, although the frequency was
similar to those for late and DL ulcers. CD8+ cells were mostly found in the
papillary dermis. Conclusions CD8+ T and granzyme B+ cells are present in the
inflammatory infiltrates of CL and DL and may participate in the immunopathogenesis
of Leishmania infection. .
AD - Dantas, Marina Loyola
Fundacao Oswaldo Cruz. Centro de Pesquisas Goncalo Moniz. Laboratorio Avancado de
Saude Publica. Salvador. BR
Oliveira, Juliana Cabral de
Carvalho, Lucas
Passos, Sara Timoteo
Queiroz, Adriano
Machado, Paulo
Carvalho, Edgar
Arruda, Sergio
AU - Dantas, Marina Loyola
AU - Oliveira, Juliana Cabral de
AU - Carvalho, Lucas
AU - Passos, Sara Timoteo
AU - Queiroz, Adriano
AU - Machado, Paulo
AU - Carvalho, Edgar
AU - Arruda, Sergio
C1 - 20140127
DA - 2013/12
DB - LILACS
DO - 10.1590/S0037-86822013000600728
IS - 6
KW - CD8
Cutaneous
Cytotoxicity
Human
Inflammation
Leishmaniasis
LA - en
PY - 2013
SN - 0037-8682
SP - 728-734
ST - CD8+ T cells in situ in different clinical forms of human cutaneous
leishmaniasis
T2 - Rev. Soc. Bras. Med. Trop
TI - CD8+ T cells in situ in different clinical forms of human cutaneous
leishmaniasis
86822013000600728
VL - 46
ID - 4941
ER -
TY - JOUR
AB - This work investigates the influence of Ag (1 wt%) on the mechanical
properties, in vitro and in vivo corrosion, and biocompatibility of Fe-35Mn. The
microstructure of Fe-35Mn-1Ag possesses a uniform dispersion of discrete silver
particles. Slight improvements in compressive properties are attributed to enhanced
density and low porosity volume. Fe-35Mn-1Ag exhibits good in vitro and in vivo
corrosion rate of Fe-35Mn due to an increase in microgalvanic corrosion. Gas
pockets, which originate from an inflammatory response to the implants, are
observed in the rats after 4 weeks implantation but are undetectable after 12
weeks. No chronic toxicity is observed with the Fe-35Mn-1Ag, suggesting acceptable
in vivo biocompatibility. The high corrosion rate of the alloy triggers an
increased level of nonadverse tissue inflammatory responses 4 weeks after
implantation, which subsequently subsides at 12 weeks. The Fe-35Mn-1Ag displays
properties that are suitable for orthopedic applications. © 2020 Wiley-VCH GmbH
AU - Dargusch, M. S.
AU - Venezuela, J.
AU - Dehghan-Manshadi, A.
AU - Johnston, S.
AU - Yang, N.
AU - Mardon, K.
AU - Lau, C.
AU - Allavena, R.
C7 - 2000667
DB - Scopus
DO - 10.1002/adhm.202000667
IS - 2
KW - biocompatibility
biodegradable metals
corrosion
iron alloys
mechanical properties
Absorbable Implants
Alloys
Animals
Corrosion
Hydrogen
Materials Testing
Rats
Silver
Binary alloys
Biocompatibility
Corrosion rate
Manganese alloys
Silver alloys
Ternary alloys
iron
manganese
silver
alloy
biomaterial
hydrogen
Bioabsorbable
Chronic toxicity
Compressive properties
Hydrogen gas evolution
Orthopedic applications
Silver particles
Uniform dispersions
Article
biodegradation
chemical structure
compression
controlled study
density
dispersion
gas evolution
histology
hydrogen gas evolution
in vitro study
in vivo study
porosity
priority journal
X ray diffraction
animal
biodegradable implant
materials testing
rat
Iron alloys
M3 - Article
PY - 2021
ST - In Vivo Evaluation of Bioabsorbable Fe-35Mn-1Ag: First Reports on In Vivo
Hydrogen Gas Evolution in Fe-Based Implants
TI - In Vivo Evaluation of Bioabsorbable Fe-35Mn-1Ag: First Reports on In Vivo
Hydrogen Gas Evolution in Fe-Based Implants
85094653318&doi=10.1002%2fadhm.202000667&partnerID=40&md5=a1ca9269917ed85647fb2f8f0
17edb20
VL - 10
ID - 5310
ER -
TY - JOUR
AB - Polymeric nanoparticles (NPs) have the potential to provide effective and
safe delivery of antiretroviral drugs in the context of prophylactic anti-HIV
vaginal microbicides. Dapivirine-loaded poly(D,L-lactic-co-glycolic acid) (PLGA)
NPs were produced by an emulsion-solvent evaporation method, optimized for
colloidal properties using a 3-factor, 3-level Box-Behnken experimental design, and
characterized for drug loading, production yield, morphology, thermal behavior,
drug release, in vitro cellular uptake, cytotoxicity and pro-inflammatory
potential. Also, drug permeability/membrane retention in well-established HEC-1-A
and CaSki cell monolayer models as mediated by NPs was assessed in the absence or
presence of mucin. Box-Behnken design allowed optimizing monodisperse 170 nm drug-
loaded NPs. Drug release experiments showed an initial burst effect up to 4 h,
followed by sustained 24 h release at pH 4.2 and 7.4. NPs were readily taken up by
different genital and macrophage cell lines as assessed by fluorescence microscopy.
Drug-loaded NPs presented lower or at least similar cytotoxicity as compared to the
free drug, with up to around one-log increase in half-maximal cytotoxic
concentration values. In all cases, no relevant changes in cell pro-inflammatory
cytokine/chemokine production were observed. Dapivirine transport across cell
monolayers was significantly decreased when mucin was present at the donor side
with either NPs or the free drug, thus evidencing the influence of this natural
glycoprotein in membrane permeability. Moreover, drug retention in cell monolayers
was significantly higher for NPs in comparison with the free drug. Overall,
obtained dapivirine-loaded PLGA NPs possess interesting technological and
biological features that may contribute to their use as novel safe and effective
vaginal microbicides. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All
rights reserved.
AN - WOS:000353605300009
AU - das Neves, J.
AU - Sarmento, B.
DA - MAY
DO - 10.1016/j.actbio.2015.02.007
PY - 2015
SN - 1742-7061
1878-7568
SP - 77-87
ST - Precise engineering of dapivirine-loaded nanoparticles for the development of
anti-HIV vaginal microbicides
TI - Precise engineering of dapivirine-loaded nanoparticles for the development of
anti-HIV vaginal microbicides
VL - 18
ID - 6651
ER -
TY - JOUR
AB - In this study, silver nanoparticles (PE-AgNPs) were synthesized using Premna
esculenta (PE) leaf extract, characterized by UV, TEM, TEM-EDX, XRD, DLS, and
ICPOES studies. AgNPs were found to be stable at -35 mV with 26.157 ppm
concentration. AgNPs were found triangular in shape with an average size of about
44 nm. Hepatoprotective activity was assessed in animal model using silymarin as
standard drug. Biochemical parameters (serum AST, ALT, gamma GT, ACP, and ALP),
inflammatory markers (IL 1 beta, IL 17, TNF alpha, and IL 10), and antioxidant
markers (GSH, SOD, Catalase, and LPO) were assayed. Liver tissue was processed for
histological analysis. Induction of hepatotoxicity increases AST, ALT, gamma GT,
ACP, ALP, IL 10, LPO, and decreases IL 1 beta, IL 17, TNF alpha, GSH, SOD, and
Catalase. PE-AgNPs treatment significantly decreases IL 10, LPO while IL 1 beta, IL
17, TNF alpha, GSH, and SOD were increased in animals. PE-AgNPs partially recovered
CCl4-induced changes in liver histology.
AN - WOS:000940303300001
AU - Das, B. K.
AU - Ghosh, S.
AU - Gomes, A.
AU - De, U. C.
C6 - FEB 2023
DA - 2023 FEB 18
DO - 10.1080/24701556.2023.2181821
PY - 2023
SN - 2470-1556
2470-1564
ST - Synthesis of silver nanoparticles using aqueous leaf extract of Premna
esculenta and in vivo evaluation of its hepatoprotective activity in Swiss albino
male mice
TI - Synthesis of silver nanoparticles using aqueous leaf extract of Premna
esculenta and in vivo evaluation of its hepatoprotective activity in Swiss albino
male mice
ID - 6086
ER -
TY - JOUR
AB - Background The advances in products based on nanotechnology have directed
extensive research on low-cost, biologically compatible, and easily degradable
materials. Main body Sericin (SER) is a protein mainly composed of glycine, serine,
aspartic acid, and threonine amino acids removed from the silkworm cocoon
(particularly Bombyx mori and other species). SER is a biocompatible material with
economic viability, which can be easily functionalized due to its potential
crosslink reactions. Also, SER has inherent biological properties, which makes
possible its use as a component of pharmaceutical formulations with several
biomedical applications, such as anti-tumor, antimicrobials, antioxidants and as
scaffolds for tissue repair as well as participating in molecular mechanisms
attributed to the regulation of transcription factors, reduction of inflammatory
signaling molecules, stimulation of apoptosis, migration, and proliferation of
mesenchymal cells. Conclusion In this review, the recent innovations on SER-based
nano-medicines (nanoparticles, micelles, films, hydrogels, and their hybrid
systems) and their contributions for non-conventional therapies are discussed
considering different molecular mechanisms for promoting their therapeutic
applications.
AN - WOS:000612942200002
AU - Das, G.
AU - Shin, H. S.
AU - Campos, E. V. R.
AU - Fraceto, L. F.
AU - Rodriguez-Torres, M. D.
AU - Mariano, K. C. F.
AU - de Araujo, D. R.
AU - Fernandez-Luqueno, F.
AU - Grillo, R.
AU - Patra, J. K.
C7 - 30
DA - JAN 22
DO - 10.1186/s12951-021-00774-y
IS - 1
PY - 2021
SN - 1477-3155
ST - Sericin based nanoformulations: a comprehensive review on molecular
mechanisms of interaction with organisms to biological applications
TI - Sericin based nanoformulations: a comprehensive review on molecular
mechanisms of interaction with organisms to biological applications
VL - 19
ID - 6733
ER -
TY - JOUR
AB - Engineered nanoparticles (ENPs) offer technological advantages for a variety
of industrial and consumer products as well as show promise for biomedical
applications. Recent progress in the field of nanotechnology has led to increased
exposure to nanoparticles by humans. To date, little is known about the adverse
effects of these ENPs on reproductive health, although interest in nanotechnology
area is growing. A few biocompatible ENPs have a high loading capacity for
exogenous substances, including drugs, DNA or proteins, and can selectively deliver
molecular cargo into cells; however, they represent a potential tool for gene
delivery into gametes and embryos. Understanding the reprotoxicological aspects of
these ENPs is of the utmost importance to reliably estimate its potential impact on
human health. In addition, a search for protective agents to combat ENP-mediated
reproductive toxicity is warranted. Therefore, in this review we summarize the
toxic effects of a few ENPs (metal and metal oxides, carbon-based nanoparticles,
quantum dots and chitosan) in mammalian germ cells and developing embryos, and
propose some treatment strategies that could mitigate nanoparticle-mediated
toxicity. In addition, we outline the anticipated applications of ENPs in
transgenic animal production in order to generate models for investigations into
the mechanisms for human disease. A literature search was performed using the
National Center for Biotechnology Information PubMed database up until March 2016
and relevant keywords were used to obtain information regarding mammalian germ
cell-specific toxicity and embryotoxicity of ENPs, possible treatment strategies,
as well as the anticipated applications of nanoparticles in gene delivery in germ
cells and embryos. Only English language publications were included. Here, we
demonstrate the toxicological effects of ENPs in mammalian germ cells and
developing embryos by considering both in vitro and in vivo experimental models
based on the existing literature. The biodistribution and cellular uptake of ENPs
and the observed toxicities are mostly dependent on ENP size and surface-coating
agents (surface functional groups/surface charge). ENPs have been shown to induce
toxicity via oxidative stress, inflammation and DNA damage in both human and mouse
germ cells. Use of antioxidant, anti-inflammatory drugs and selective metal
chelators would be beneficial against nanoparticle-induced toxicity. Our review
provides the reproductive scientists a mechanistic insight into the
reprotoxicological aspects of ENPs to reliably estimate its potential impact on
human health and help to select/design protective agents to combat ENP-mediated
toxicity. Furthermore, research regarding the detailed mechanism(s) of ENP toxicity
in mammalian germ cells and developing embryos as well as the search for protective
agents to combat ENP-mediated reproductive toxicity is warranted. Furthermore, we
anticipate that investigations into the possibility of applying nanovectors to gene
delivery in germ cells and early embryos will open new horizons in reproductive
biology.
AN - WOS:000383902200004
AU - Das, J.
AU - Choi, Y. J.
AU - Song, H.
AU - Kim, J. H.
DA - SEP-OCT
DO - 10.1093/humupd/dmw020
IS - 5
PY - 2016
SN - 1355-4786
1460-2369
SP - 588-619
ST - Potential toxicity of engineered nanoparticles in mammalian germ cells and
developing embryos: treatment strategies and anticipated applications of
nanoparticles in gene delivery
T2 - HUMAN REPRODUCTION UPDATE
TI - Potential toxicity of engineered nanoparticles in mammalian germ cells and
developing embryos: treatment strategies and anticipated applications of
nanoparticles in gene delivery
VL - 22
ID - 6292
ER -
TY - JOUR
AB - Yersinia pestis, the causative agent of plague, is known to develop
strategies to overcome the host immune mechanisms and survive in the host. The
molecular changes induced by Y. pestis in the host are not well delineated. Here,
we examined the early events triggered after the intracellular infection of Y.
pestis in human monocytes and lymphocytes by analyzing the host transcriptional
profiles using cDNA arrays. We found that sets of genes that, especially at early
time periods, were highly upregulated in monocytes alone when compared with a mixed
culture of lymphocytes and monocytes. Gene expression responses revealed genes
coding for cytokines, chemokines, transcription factors, inflammatory and
apoptosis-related genes. Protein levels were measured, and real-time polymerase
chain reaction was used to validate the microarray results. Our data suggest that
intracellular infection of human monocytes with Y. pestis results in a strong
inflammatory response at early time periods and a downregulation of genes such as
thromobomodulin, which may play a role in coagulation, resulting in disseminated
intravascular coagulation, a primary cause of death in plague infected hosts. We
provide evidence that genomic analysis can provide a solid foundation to
mechanistic insights to explain some of the symptoms induced by Y. pestis.
AN - WOS:000247115900005
AU - Das, R.
AU - Dhokalia, A.
AU - Huang, Z.
AU - Hammamieh, R.
AU - Chakraborty, N.
AU - Linder, L. E.
AU - Jett, M.
DA - JUN
DO - 10.1038/sj.gene.6364389
IS - 4
PY - 2007
SN - 1466-4879
1476-5470
SP - 308-319
ST - Study of proinflammatory responses induced by Yersinia pestis in human
monocytes using cDNA arrays
T2 - GENES AND IMMUNITY
TI - Study of proinflammatory responses induced by Yersinia pestis in human
monocytes using cDNA arrays
VL - 8
ID - 6819
ER -
TY - JOUR
AB - This research aimed at reporting the synthesis, characterization and
evaluation of the anti-inflammatory effects of some new biomaterials based on
silver nanoparticles and polyphenols rich natural extracts. A fast and eco-friendly
extracellular biosynthesis of silver nanoparticles (AgNPs), using European black
elderberry (Sambucus nigra - SN, Adoxaceae family) fruit extracts was developed.
The phytosynthesized nanoparticles exhibited an absorbance peak at 426nm,
characteristic for AgNPs and their sizes were ranged from 20 to 80 nm. The anti-
inflammatory properties of AgNPs were assessed in vitro on HaCaT cells exposed to
UVB radiation, in vivo on acute inflammation model and in humans on psoriasis
lesions. In vitro, our results demonstrated the anti-inflammatory effects of
functionalized AgNPs by the decrease of cytokines production induced by UVB
irradiation. In vivo, the pre-administration of AgNPs reduced the edema and
cytokines levels in the paw tissues, early after the induction of inflammation. The
present study also demonstrated the possible use of synthesized AgNPs for the
treatment of psoriasis lesions. (C) 2014 Elsevier B.V. All rights reserved.
AN - WOS:000343612900096
AU - David, L.
AU - Moldovan, B.
AU - Vulcu, A.
AU - Olenic, L.
AU - Perde-Schrepler, M.
AU - Fischer-Fodor, E.
AU - Florea, A.
AU - Crisan, M.
AU - Chiorean, I.
AU - Clichici, S.
AU - Filip, G. A.
DA - OCT 1
DO - 10.1016/j.colsurfb.2014.08.018
PY - 2014
SN - 0927-7765
1873-4367
SP - 767-777
ST - Green synthesis, characterization and anti-inflammatory activity of silver
nanoparticles using European black elderberry fruits extract
TI - Green synthesis, characterization and anti-inflammatory activity of silver
nanoparticles using European black elderberry fruits extract
VL - 122
ID - 5840
ER -
TY - JOUR
AB - Cisplatin (CDDP) is a well-known platinum-based drug used in the treatment of
various malignancies. However, the widespread side effects that this drug leaves on
normal tissues make its use limited. Since cisplatin is mainly eliminated from the
kidneys, CDDP-induced nephrotoxicity is the most significant dose-limiting
complication attributed to cisplatin, which often leads to dose withdrawal.
Considering the high efficiency of cisplatin in chemotherapy, finding
renoprotective drug delivery systems for this drug is a necessity. In this regard,
we can take advantages of different nanoparticle-based approaches to deliver
cisplatin into tumors either using passive targeting or using specific receptors.
In an effort to find more effective cisplatin-based nano-drugs with less
nephrotoxic effect, the current 2011-2022 review study was conducted to investigate
some of the nanotechnology-based methods that have successfully been able to
mitigate CDDP-induced nephrotoxicity. Accordingly, although cisplatin can cause
renal failures through inducing mitochondria dysfunction, oxidative stress, lipid
peroxidation and endoplasmic reticulum stress, some CDDP-based nano-carriers have
been able to reverse a wide range of these advert effects. Based on the obtained
results, it was found that the use of different metallic and polymeric
nanoparticles can help renal cells to strengthen their antioxidant systems and stay
alive through reducing CDDP-induced ROS generation, inhibiting apoptosis-related
pathways and maintaining the integrity of the mitochondrial membrane. For example,
nanocurcumin could inhibit oxidative stress and acting as a ROS scavenger. CONPs
could reduce lipid peroxidation and pro-inflammatory cytokines. CDDP-loaded silver
nanoparticles (AgNPs) could inhibit mitochondria-mediated apoptosis. In addition,
tea polyphenol-functionalized SeNPs (Se@TE) NPs could mitigate the increased level
of dephosphorylated AKT, phosphorylated p38 MAPK and phosphorylated c-Jun N-
terminal kinase (JNK) induced by cisplatin. Moreover, exosomes mitigated cisplatin-
induced renal damage through inhibiting Bcl2 and increasing Bim, Bid, Bax, cleaved
caspase-9, and cleaved caspase-3. Hence, nanoparticle-based techniques are
promising drug delivery systems for cisplatin so that some of them, such as
lipoplatins and nanocurcumins, have even reached phases 1-3 trials.
AN - WOS:000893065500005
AU - Davoudi, M.
AU - Jadidi, Y.
AU - Moayedi, K.
AU - Farrokhi, V.
AU - Afrisham, R.
C7 - 504
DA - DEC 1
DO - 10.1186/s12951-022-01718-w
IS - 1
PY - 2022
SN - 1477-3155
ST - Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-
induced nephrotoxicity: a 2011-2022 review
TI - Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-
induced nephrotoxicity: a 2011-2022 review
VL - 20
ID - 6496
ER -
TY - JOUR
AB - Cisplatin (CDDP) is a well-known platinum-based drug used in the treatment of
various malignancies. However, the widespread side effects that this drug leaves on
normal tissues make its use limited. Since cisplatin is mainly eliminated from the
kidneys, CDDP-induced nephrotoxicity is the most significant dose-limiting
complication attributed to cisplatin, which often leads to dose withdrawal.
Considering the high efficiency of cisplatin in chemotherapy, finding
renoprotective drug delivery systems for this drug is a necessity. In this regard,
we can take advantages of different nanoparticle-based approaches to deliver
cisplatin into tumors either using passive targeting or using specific receptors.
In an effort to find more effective cisplatin-based nano-drugs with less
nephrotoxic effect, the current 2011–2022 review study was conducted to investigate
some of the nanotechnology-based methods that have successfully been able to
mitigate CDDP-induced nephrotoxicity. Accordingly, although cisplatin can cause
renal failures through inducing mitochondria dysfunction, oxidative stress, lipid
peroxidation and endoplasmic reticulum stress, some CDDP-based nano-carriers have
been able to reverse a wide range of these advert effects. Based on the obtained
results, it was found that the use of different metallic and polymeric
nanoparticles can help renal cells to strengthen their antioxidant systems and stay
alive through reducing CDDP-induced ROS generation, inhibiting apoptosis-related
pathways and maintaining the integrity of the mitochondrial membrane. For example,
nanocurcumin could inhibit oxidative stress and acting as a ROS scavenger. CONPs
could reduce lipid peroxidation and pro-inflammatory cytokines. CDDP-loaded silver
nanoparticles (AgNPs) could inhibit mitochondria-mediated apoptosis. In addition,
tea polyphenol-functionalized SeNPs (Se@TE) NPs could mitigate the increased level
of dephosphorylated AKT, phosphorylated p38 MAPK and phosphorylated c-Jun N-
terminal kinase (JNK) induced by cisplatin. Moreover, exosomes mitigated cisplatin-
induced renal damage through inhibiting Bcl2 and increasing Bim, Bid, Bax, cleaved
caspase-9, and cleaved caspase-3. Hence, nanoparticle-based techniques are
promising drug delivery systems for cisplatin so that some of them, such as
lipoplatins and nanocurcumins, have even reached phases 1–3 trials. © 2022, The
Author(s).
AU - Davoudi, M.
AU - Jadidi, Y.
AU - Moayedi, K.
AU - Farrokhi, V.
AU - Afrisham, R.
C7 - 504
DB - Scopus
DO - 10.1186/s12951-022-01718-w
IS - 1
KW - Anti-apoptosis
Antioxidant
Cisplatin
Kidney
Metallic nanoparticles
Nephroprotection
Nephrotoxicity
Polymers
Metal Nanoparticles
Silver
Antioxidants
Chemotherapy
Drug dosage
Drug interactions
Lipids
Mitochondria
Oxidation
Oxidative stress
Phosphorylation
albumin
alginic acid
BIM protein
boldine
calcium carbonate
carbon monoxide
caspase 3
caspase 9
chitosan derivative
cisplatin
curcumin
cytokine
drug carrier
eudragit rs
gallic acid
gelatin
gold nanoparticle
honokiol
hyaluronic acid
lipid
liposome
macrogol
metal nanoparticle
microsphere
n,n' diphenyl 1,4 phenylenediamine
poloxamer
polyglactin
polymer nanoparticle
polymethacrylic acid
polyphenol
polystyrene
protein Bax
protein bcl 2
protein Bid
protein kinase B
silk fibroin
silver nanoparticle
small interfering RNA
stress activated protein kinase
trolox C
polymer
silver
Cis-platin
Lipid peroxidation
antiapoptotic activity
apoptosis
Article
cell survival
dephosphorylation
enzyme phosphorylation
exosome
human
inhibition kinetics
kidney cell
kidney disease
kidney failure
lipid peroxidation
nanotechnology
nonhuman
oxidative stress
particle size
protein dephosphorylation
renal protection
sustained release formulation
zeta potential
Cell death
M3 - Article
PY - 2022
ST - Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-
induced nephrotoxicity: a 2011–2022 review
T2 - Journal of Nanobiotechnology
TI - Ameliorative impacts of polymeric and metallic nanoparticles on cisplatin-
induced nephrotoxicity: a 2011–2022 review
85143181343&doi=10.1186%2fs12951-022-01718-
w&partnerID=40&md5=7c983268c7970ea7577e667d9b121dec
VL - 20
ID - 4998
ER -
TY - JOUR
AB - SUMMARY: Rheumatoid arthritis (RA) is considered an autoimmune disease
distinguished by chronic synovial membrane inflammation, degraded cartilage, as
well as bone destruction, which lead to joints pain and stiffness. The pathogenesis
of RA involved at least two mechanisms: Cellular proliferation and activation of
glycogen synthase kinase-3β (GSK3β) enzyme. Thus, we tested the hypothesis that the
GSK3binhibitor, TDZD-8, can treat the synovial tissue toward collagen type II
(COII)-mediated RA linked to apoptosis induction and biomarker suppression of
inflammation. Wistar rats were immunized with COII (the model group) for 21 days.
Matched immunized rats were daily injected with TDZD-8 (1 mg/kg; i.p) for three
additional weeks (COII+TDZD- 8).After 42 days of post-immunization, blood and
tissues were collected. Histology (H&E) and immunohistochemistry (CD45; leukocyte
common antigen) images showed that COII induced RA was demonstrated by profound
damage to the synovial tissue and infiltration of the inflammatory cells, which
were substantially ameliorated with TDZD-8. In addition, COII immunization caused
the induction of rheumatoid factor (RF), tumor necrosis factor-alpha (TNF-α),
interleukin-6 (IL-6), and interleukin 1 beta (IL-1β) that were substantially
(p<0.05) suppressed by TDZD-8. Whereas, TDZD-8 augmented the apoptotic biomarker,
Bcl-2-associated X protein (Bax), which was significantly (p<0.05) ameliorated by
RA. We also showed a substantial relationship (p<0.001) between the blood levels of
RF and the synovial tissue levels of TNF-α (r = 0.759), IL-1b (r = 0.969), IL-6 (r
= 0.749), and Bax (r = - 0.914). These results indicate effective treatment of the
injured synovial tissue by TDZD-8 against COII-induced RA in rats, which also
decreases inflammatory biomarkers and augmentation of apoptosis.
RESUMEN: La artritis reumatoide (AR) es una enfermedad autoinmune que se distingue
por la inflamación crónica de la membrana sinovial, el cartílago degradado y la
destrucción de los huesos, lo que provoca dolor y rigidez en las articulaciones. La
patogenia de la AR involucra al menos dos mecanismos: la proliferación celular y la
activación de la enzima glucógeno sintasa quinasa-3b (GSK3β) Por lo tanto, probamos
la hipótesis de que el inhibidor de GSK3β, TDZD-8, puede tratar el tejido sinovial
hacia el colágeno tipo II (COII) - AR mediada por inducción de apoptosis y
supresión de biomarcadores de inflamación. Se inmunizaron ratas Wistar con COII (el
grupo modelo) durante 21 días. Se inyectaron diariamente ratas emparejadas
inmunizadas con TDZD-8 (1 mg / kg; i.p) durante tres semanas adicionales (COII +
TDZD-8). Después de 42 días de post-inmunización, se recolectó sangre y tejidos.
Las imágenes de histología (H&E) e inmunohistoquímica (CD45; antígeno común de
leucocitos) mostraron que la AR inducida por COII presentaba un daño profundo en el
tejido sinovial e infiltración de las células inflamatorias, las que mejoraron con
TDZD-8. Además, la inmunización con COII provocó la inducción de factor reumatoide
(FR), factor de necrosis tumoral alfa (TNF-α), interleucina-6 (IL-6) e interleucina
1 beta (IL-1β) que fueron suprimidos por TDZD-8 de manera significativa (p < 0.05).
Considerando que TDZD-8 aumentó el biomarcador apoptótico, la proteína X asociada a
Bcl-2 (Bax), que fue mejorado (p <0,05) por RA. También se observó una relación
sustancial (p <0,001) entre los niveles sanguíneos de RF y los niveles de tejido
sinovial de TNF-α (r = 0,759), IL-1β (r = 0,969), IL-6 (r = 0,749), y Bax (r = -
0,914). Estos resultados indicaron un tratamiento eficaz del tejido sinovial
lesionado por TDZD-8 contra la AR inducida por COII en ratas, que también disminuye
los biomarcadores inflamatorios y el aumento de la apoptosis.
AD - Dawood, Amal F
Princess Nourah Bint Abdulrahman University. College of Medicine. Department of
Basic Medical Sciences. Riyadh. SA
AU - Dawood, Amal F.
C1 - 20220812
DA - 2021/02
DB - LILACS
DO - 10.4067/S0717-95022021000100311
IS - 1
KW - Artritis reumatoide
Bax
Inflamación
Inflammation
Modelo de rata
Rat model
TDZD-8
LA - en
PY - 2021
SN - 0717-9367
SP - 311-317
ST - TDZD-8 suppresses rheumatoid arthritis induced by collagen type II in rats:
potential role of apoptosis and inflammation
TI - TDZD-8 suppresses rheumatoid arthritis induced by collagen type II in rats:
potential role of apoptosis and inflammation
TT - TDZD-8 suprime la artritis reumatoide inducida por colágeno tipo II en ratas:
papel potencial de la apoptosis y la inflamación
VL - 39
ID - 4920
ER -
TY - JOUR
AB - Background/Aims: A new type of nanoparticle, called NP CB-EDA (Black Carbon
modified with ethylenediamine), is commonly used in the oil industry. In the
literature, few studies are found in biological models, making NP-EDA potential
cytotoxicity in organisms unclear. As its large surface area is capable of
interacting with the biological system, that interaction could lead to factors
harmful to health. The objective of this study was to investigate the cytotoxic
effect of NP CB-EDA on fibroblasts LA-9 at 24 and 48 hours, at different
concentrations of the nanoparticle (1, 50, 250, 500 and 1000 μg/ml). Methods: NP
CB-EDA was characterized by TEM microscopy and its effect on cell viability (MTT
method), cell morphology (optical microscopy), cell membrane (lactate dehydrogenase
release - LDH), oxidative stress pathways (species levels reactive oxygen, ROS and
nitrogen, NOS) and apoptosis/necrosis (flow cytometry) were evaluated. Results: The
results show that NP CB-EDA at concentrations of 500 and 1000 μg/ ml form clusters.
The nanoparticle can be absorbed by cells decreasing cell viability. There was
damage to the cell membrane of fibroblasts LA 9, an increase in the production of
ROS, NOS and pro-inflammatory interleukins TNF-a and IL-6; it was also observed an
increase in % of cells in the state of apoptosis in the two periods analyzed, being
this response more significant in 24 hours, and concentrations of 250, 500 and 1000
μg/ml presenting higher cytotoxicity. Conclusion: The data suggest that NP CB-EDA
in fibroblasts LA9 presents cytotoxic potential, which is associated with oxidative
stress and apoptosis. © 2021 The Author(s).
AU - De Almeida Rodolpho, J. M.
AU - De Godoy, K. F.
AU - Brassolatti, P.
AU - De Lima Fragelli, B. D.
AU - De Castro, C. Aq
AU - Assis, M.
AU - Speglich, C.
AU - Cancino-Bernardi, J.
AU - Longo, E.
AU - De Freitas Anibal, F.
DB - Scopus
DO - 10.33594/000000382
IS - 3
KW - Apoptosis
Carbon black
Cell viability
Nanoparticle
Oxidative stress
ROS/RNS
Animals
Cell Line
Cytotoxins
Fibroblasts
Mice
Nanoparticles
Oxidative Stress
Soot
carbon nanoparticle
caspase 3
CD4 antigen
interleukin 6
silver nanoparticle
cytotoxin
nanoparticle
apoptosis
Article
atherogenesis
cell viability
clonogenic assay
colony formation
controlled study
cytotoxicity
EC50
fibroblast
flow cytometry
human
human cell
lung embolism
microscopy
MTT assay
nonhuman
oxidative stress
particle size
zeta potential
animal
cell line
drug effect
metabolism
mouse
pharmacology
soot
M3 - Article
PY - 2021
SP - 364-377
ST - Apoptosis and oxidative stress triggered by carbon black nanoparticle in the
LA-9 fibroblast
T2 - Cellular Physiology and Biochemistry
TI - Apoptosis and oxidative stress triggered by carbon black nanoparticle in the
LA-9 fibroblast
85109635875&doi=10.33594%2f000000382&partnerID=40&md5=ee7245fc78db6792a398ab029fb6a
e66
VL - 55
ID - 5221
ER -
TY - JOUR
AB - Engineered nanoparticles offer great promise in many industrial and
biomedical applications, however little information is available about
gastrointestinal toxicity The purpose of this study was to assess the cytotoxicity,
oxidative stress, apoptosis and proinflammatory mediator release induced by ZnO
nanoparticles on human colon carcinoma LoVo cells The biological activity of these
particles was related to their physico-chemical characteristics The physico-
chemical characteristics were evaluated by analytical electron microscopy The
cytotoxicity was determined by growth curves and water-soluble tetrazolium assay
The reactive oxygen species production, cellular glutathione content, changes of
mitochondrial membrane potential and apoptosis cell death were quantified by flow
cytometry. The inflammatory cytokines were evaluated by enzyme-linked
immunoadsorbent assay Treatment with ZnO (5 mu g/cm(2) corresponding to 11 5 mu
g/ml) for 24 h induced on LoVo cells a significant decrease of cell viability,
H2O2/OH center dot increase, 02(-center dot) and GSH decrease, depolarization of
inner mitochondrial membranes, apoptosis and IL-8 release Higher doses induced
about 98% of cytotoxicity already after 24 h of treatment The experimental data
show that oxidative stress may be a key route in inducing the cytotoxicity of ZnO
nanoparticles in colon carcinoma cells Moreover, the study of the relationship
between toxicological effects and physico-chemical characteristics of particles
suggests that surface area does not play a primary role in the cytotoxicity (C)
2010 Elsevier Inc All rights reserved
AN - WOS:000280042700002
AU - De Berardis, B.
AU - Civitelli, G.
AU - Condello, M.
AU - Lista, P.
AU - Pozzi, R.
AU - Arancia, G.
AU - Meschini, S.
DA - AUG 1
DO - 10.1016/j.taap.2010.04.012
IS - 3
PY - 2010
SN - 0041-008X
1096-0333
SP - 116-127
ST - Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in
human colon carcinoma cells
T2 - TOXICOLOGY AND APPLIED PHARMACOLOGY
TI - Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in
human colon carcinoma cells
VL - 246
ID - 6187
ER -
TY - JOUR
AB - Bioactive glasses (BG) applications include tissue engineering for bone
regeneration, coating for implants, and scaffolds for wound healing. BG can be
conjugated to ions like silver, which might add some antimicrobial properties to
this biomaterial. The immunomodulatory activity of ion-doped bioactive glasses
particles was not investigated before. The aim of this work was to evaluate the
cytotoxic and immunomodulatory effect of BG and silver-doped bioactive glass (BGAg)
in human peripheral blood cells. BG and BGAg samples belonging to the system
58SiO(2)center dot(36-x)CaO6P(2)O(5)xAg(2)O, where x = 0 and 1 mol%, respectively,
were synthesized via sol-gel method and characterized. Cytotoxicity, modulation of
cytokine production (TNF-, IL-1, IL-6, IL-4, and IL-10), and oxidative stress
response were investigated in human polymorphonuclear cells (PMNs) and peripheral
blood mononuclear cells (PBMCs) cultures. Cell viability in the presence of BG or
BGAg was concentration-dependent. In addition, BGAg presented higher PBMCs toxicity
(LC50 = 0.005%) when compared to BG (LC50 = 0.106%). Interestingly, interleukin4
was produced by PBMCs in response to BG and BGAg in absence of phytohemagglutinin
(PHA) and did not modulate PHA-induced cytokine levels. Subtoxic concentrations
(0.031% for BG and 0.0008% for BGAg) did not change other cytokines in PBMCs nor
reactive oxygen species (ROS) production by PMN. However, BG and BGAg particles
decreased zymosan-induced ROS levels in PMN. Although ion incorporation increased
BG cytotoxicity, the bioactive glass particles demonstrated a in vitro anti-
inflammatory potencial. Future studies are needed to clarify the scavenger
potential of the BG/BGAg particles/scaffolds as well as elucidate the effect of the
anti-inflammatory potential in modulating tissue growth in vivo.
AN - WOS:000472831700001
AU - de Lima, J. M.
AU - Ferreira, E. P.
AU - Bonan, R. F.
AU - Silva-Teixeira, D. N.
AU - Goulart, L. R.
AU - de Souza, J. R.
AU - de Medeiros, E. S.
AU - Bonan, P. R. F.
AU - Castellano, L. R. C.
C7 - 3210530
DO - 10.1155/2019/3210530
PY - 2019
SN - 2314-6133
2314-6141
ST - Cytokine Regulation from Human Peripheral Blood Leukocytes Cultured In Vitro
with Silver Doped Bioactive Glasses Microparticles
T2 - BIOMED RESEARCH INTERNATIONAL
TI - Cytokine Regulation from Human Peripheral Blood Leukocytes Cultured In Vitro
VL - 2019
ID - 6284
ER -
TY - JOUR
AB - In recent years interest in silver nanoparticles and their applications has
increased mainly because of the important antimicrobial activities of these
nanomaterials, allowing their use in several industrial sectors. However, together
with these applications, there is increasing concerning related to the biological
impacts of the use of silver nanoparticles on a large scale, and the possible risks
to the environment and health. In this scenario, some recent studies have been
published based on the investigation of potential inflammatory effects and diverse
cellular impacts of silver nanoparticles. Another important issue related to
nanoparticle toxicity in biological media is the capacity for increased damage to
the genetic material, since nanoparticles are able to cross cell membranes and
reach the cellular nucleus. In this regard, there is increasing interest in the
analysis of potential nanoparticle genotoxicity, including the effects of different
nanoparticle sizes and methods of synthesis. However, little is known about the
genotoxicity of different silver nanoparticles and their effects on the DNA of
organisms; thus further studies in this field are required. This mini-review aims
to present and to discuss recent publications related to genotoxicity and the
cytotoxicity of silver nanoparticles in order to better understand the possible
applications of these nanomaterials in a safe manner. This present work concludes
that biogenic silver nanoparticles are generally less cyto/genotoxic in vivo
compared with chemically synthesized nanoparticles. Furthermore, human cells were
found to have a greater resistance to the toxic effects of silver nanoparticles in
comparison with other organisms. Copyright © 2012 John Wiley & Sons, Ltd. There is
increasing concerning related to the biological impacts of silver nanoparticles. In
this regard, there is increasing interest in the analysis of potential nanoparticle
genotoxicity, including the effects of different nanoparticle sizes and methods of
synthesis. This mini-review aims to present and to discuss recent publications
related to genotoxicity and the cytotoxicity of silver nanoparticles in order to
better understand the possible applications of these nanomaterials in a safe
manner. © 2012 John Wiley & Sons, Ltd.
AU - de Lima, R.
AU - Seabra, A. B.
AU - Durán, N.
DB - Scopus
DO - 10.1002/jat.2780
IS - 11
KW - Biogenic nanoparticle
Cytotoxicity
Genotoxicity
Nanobiotechnology
Nanotechnology
Nanotoxicology
Silver nanoparticle
Animals
Humans
Invertebrates
Metal Nanoparticles
Mice
Mutagens
Rats
Silver
Vertebrates
DNA
nanomaterial
silver nanoparticle
article
cell membrane
cell nucleus
cytotoxicity
genotoxicity
in vivo study
inflammation
medical literature
nonhuman
particle size
priority journal
synthesis
M3 - Article
PY - 2012
SP - 867-879
ST - Silver nanoparticles: A brief review of cytotoxicity and genotoxicity of
chemically and biogenically synthesized nanoparticles
TI - Silver nanoparticles: A brief review of cytotoxicity and genotoxicity of
84866847776&doi=10.1002%2fjat.2780&partnerID=40&md5=ac9a53867f4c843f7d362a8f9867cd6
9
VL - 32
ID - 5675
ER -
TY - JOUR
AB - In recent years interest in silver nanoparticles and their applications has
increased mainly because of the important antimicrobial activities of these
nanomaterials, allowing their use in several industrial sectors. However, together
with these applications, there is increasing concerning related to the biological
impacts of the use of silver nanoparticles on a large scale, and the possible risks
to the environment and health. In this scenario, some recent studies have been
published based on the investigation of potential inflammatory effects and diverse
cellular impacts of silver nanoparticles. Another important issue related to
nanoparticle toxicity in biological media is the capacity for increased damage to
the genetic material, since nanoparticles are able to cross cell membranes and
reach the cellular nucleus. In this regard, there is increasing interest in the
analysis of potential nanoparticle genotoxicity, including the effects of different
nanoparticle sizes and methods of synthesis. However, little is known about the
genotoxicity of different silver nanoparticles and their effects on the DNA of
organisms; thus further studies in this field are required. This mini-review aims
to present and to discuss recent publications related to genotoxicity and the
cytotoxicity of silver nanoparticles in order to better understand the possible
applications of these nanomaterials in a safe manner. This present work concludes
that biogenic silver nanoparticles are generally less cyto/genotoxic in vivo
compared with chemically synthesized nanoparticles. Furthermore, human cells were
found to have a greater resistance to the toxic effects of silver nanoparticles in
comparison with other organisms. Copyright (c) 2012 John Wiley & Sons, Ltd.
AN - WOS:000309190200001
AU - de Lima, R.
AU - Seabra, A. B.
AU - Duran, N.
DA - NOV
DO - 10.1002/jat.2780
IS - 11
PY - 2012
SN - 0260-437X
1099-1263
SP - 867-879
ST - Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of
TI - Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of
VL - 32
ID - 5895
ER -
TY - JOUR
AB - Graphene oxide (GO) and silver nanoparticles (AgNPs) can be formed into a
hybrid nanomaterial, known as GOAg nanocomposite, which presents high antibacterial
activity. The successful translation of this nanomaterial into medical use depends
on critical information about its toxicological profile. In keeping with a Safe-by-
design approach, we evaluated the immunotoxicity of GOAg using J774 and primary
murine macrophages. The interaction between GOAg and macrophages was investigated
with a scanning electron microscope (SEM). High-throughput technologies were
employed to evaluate cell viability, apoptosis/necrosis, mitochondrial
depolarization and lipid peroxidation. The inflammogenicity of nanomaterials was
predicted after quantification of the cytokines IL-1β, TNF-α and IL-10 before and
after stimulation with interferon-γ (IFN-γ). The ratio between CD80 and CD206
macrophage populations were also estimated. In addition, the production of nitric
oxide (NO) was investigated. SEM surveys revealed the potential of GOAg to induce
frustrated phagocytosis. GOAg induced a dose-dependent mitochondrial
depolarization, apoptosis and lipid peroxidation to J774 macrophages. GOAg toxicity
was not modified in an inflammatory microenvironment, but its toxicity was within
the range of concentrations used in bacterial inactivation. GOAg did not induce
primary macrophages to significantly produce inflammatory cytokines, and previous
macrophage stimulation did not enhance GOAg inflammogenicity. Additionally, the
pristine nanomaterials and GOAg do not shift macrophages polarization towards M1.
Sublethal concentrations of GOAg did not impair macrophages NO production. Finally,
we suggest options for improvement of GOAg nanocomposite in ways that may help
minimize its possible adverse outcomes to human health. © 2018, © 2018 Informa UK
AU - de Luna, L. A. V.
AU - Zorgi, N. E.
AU - de Moraes, A. C. M.
AU - da Silva, D. S.
AU - Consonni, S. R.
AU - Giorgio, S.
AU - Alves, O. L.
DB - Scopus
DO - 10.1080/17435390.2018.1537410
IS - 2
KW - Graphene oxide
immune system
inflammation
nanosafety
Animals
Apoptosis
Cell Line
Cell Survival
Cytokines
Graphite
Humans
Macrophages
Mice
Mice, Inbred BALB C
Nanocomposites
Phagocytosis
Silver
Surface Properties
B7 antigen
gamma interferon
graphene oxide
interleukin 10
interleukin 1beta
lymphocyte antigen
nanocomposite
nitric oxide
silver nanoparticle
antiinfective agent
cytokine
graphite
silver
animal cell
apoptosis
apoptosis rate
Article
CD206 antigen
cell membrane depolarization
cell polarity
cell population
cell viability
controlled study
cytokine production
cytotoxicity
immunotoxicity
in vitro study
internalization
J774 cell line
limit of detection
limit of quantitation
lipid peroxidation
nonhuman
phagocytosis
priority journal
surface charge
zeta potential
animal
Bagg albino mouse
cell line
cell survival
drug effect
human
immunology
macrophage
mouse
surface property
M3 - Article
PY - 2019
SP - 189-203
ST - In vitro immunotoxicological assessment of a potent microbicidal
nanocomposite based on graphene oxide and silver nanoparticles
T2 - Nanotoxicology
TI - In vitro immunotoxicological assessment of a potent microbicidal
nanocomposite based on graphene oxide and silver nanoparticles
85057555694&doi=10.1080%2f17435390.2018.1537410&partnerID=40&md5=d8d341ef26782df4c2
4cf656869519ca
VL - 13
ID - 5395
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs), silver oxide nanoparticles (AgO-NPs), and zinc
oxide nanoparticles (ZnO-NPs) have healing, antibacterial, and antioxidant
properties. Furthermore, Ag-NPs and ZnO-NPs also have antiinflammatory properties.
In this study, we synthesized a nanocomposite using Ag-ZnO and AgO-NPs (Ag-ZnO/ AgO
NPs). The structural and morphological properties of nanocrystals and nanocomposite
were investigated by X-ray diffraction and scanning electronics microscopic. The
wurtzite crystalline structure of Ag-ZnO and two morphologies for the nanocomposite
(nanorods and nanoplatelets) were determined. Topical treatment with 1% Ag-ZnO/AgO
NPs was compared to untreated wounds (control group). Wounds were induced in the
dorsal region of BALB/c mice and evaluated after 3, 7, 14, and 21 days of
treatment. The nanocomposite demonstrated antiinflammatory and antioxidant
capacities. In addition, wounds treated with Ag-ZnO/AgO NPs showed accelerated
closure, non-cytotoxicity, especially on keratinocytes and collagen deposition, and
increased metalloproteinases 2 and 9 activity. The nanocomposite improved healing
by reducing the inflammatory process, protecting tissues from damage caused by free
radicals, and increasing collagen deposition in the extracellular matrix. These
characteristics contributed to the accelerated wound closure process. Thus, Ag-
ZnO/AgO NPs show potential for can be a strategy for topical use in formulations of
new drugs to treat wounds.
AN - WOS:000780821600002
AU - de Moura, F. B. R.
AU - Ferreira, B. A.
AU - Muniz, E. H.
AU - Justino, A. B.
AU - Silva, A. G.
AU - Ribeiro, Rimd
AU - Dantas, N. O.
AU - Ribeiro, D. L.
AU - Araujo, F. D.
AU - Espindola, F. S.
AU - Silva, A. C. A.
AU - Tomiosso, T. C.
C6 - MAR 2022
C7 - 121620
DA - APR 5
DO - 10.1016/j.ijpharm.2022.121620
PY - 2022
SN - 0378-5173
1873-3476
ST - Antioxidant, anti-inflammatory, and wound healing effects of topical silver-
T2 - INTERNATIONAL JOURNAL OF PHARMACEUTICS
TI - Antioxidant, anti-inflammatory, and wound healing effects of topical silver-
VL - 617
ID - 6091
ER -
TY - JOUR
AB - Oxidative stress has increasingly been demonstrated as playing a key role in
the biological response induced by nanoparticles (NPs). The acellular cytochrome c
oxidation assay has been proposed to determine the intrinsic oxidant-generating
capacity of NPs. Yet, there is a need to improve this method to allow a rapid
screening to classify NPs in terms of toxicity. We adapted the cytochrome c assay
to take into account NP interference, to improve its sensitivity and to develop a
high-throughput method. The intrinsic oxidative ability of a panel of NPs (carbon
black, Mn2O3, Cu, Ag, BaSO4, CeO2, TiO2 and ZnO) was measured with this enhanced
test and compared to other acellular redox assays. To assess whether their
oxidative potential correlates with cellular responses, we studied the effect of
insoluble NPs on the human bronchial epithelial cell line NCI-H292 by measuring the
cytotoxicity (WST-1 assay), pro-inflammatory response (IL-8 cytokine production and
expression) and antioxidant defense induction (SOD2 and HO-1 expression). The
adapted cytochrome c assay had a greatly increased sensitivity allowing the ranking
of NPs in terms of their oxidative potential by using the developed high-throughput
technique. Besides, a high oxidative potential revealed to be predictive for toxic
effects as Mn2O3 NPs induced a strong oxidation of cytochrome c and a dose-
dependent cytotoxicity, pro-inflammatory response and antioxidant enzyme
expression. BaSO4, which presented no intrinsic oxidative potential, had no
cellular effects. Nevertheless, CeO2 and TiO2 NPs demonstrated no acellular
oxidant-generating capacity but induced moderate cellular responses. In conclusion,
the novel cytochrome c oxidation assay could be used for high-throughput screening
of the intrinsic oxidative potential of NPs. However, acellular redox assays may
not be sufficient to discriminate among low-toxicity NPs, and additional tests are
thus needed. © 2016, Springer-Verlag Berlin Heidelberg.
AU - Delaval, M.
AU - Wohlleben, W.
AU - Landsiedel, R.
AU - Boland, S.
DB - Scopus
DO - 10.1007/s00204-016-1701-3
IS - 1
KW - Barium sulfate
Carbon black
Cell culture
Cerium
Copper
In vitro
Lung
Manganese
Nanomaterial
Nanotoxicity
Nanotoxicology
Oxide
Pulmonary
Silver
Titanium
Zinc
Animals
Bronchi
Cell Line
Cell Survival
Chemical Phenomena
Cytochromes c
High-Throughput Screening Assays
Horses
Humans
Indicators and Reagents
Metal Nanoparticles
Oxidants
Oxidation-Reduction
Oxidative Stress
Particle Size
Reproducibility of Results
Respiratory Mucosa
Surface Properties
Toxicity Tests
barium sulfate
copper nanoparticle
cytochrome c
heme oxygenase 2
interleukin 8
manganese oxide
manganese superoxide dismutase
nanoparticle
silver nanoparticle
dyes, reagents, indicators, markers and buffers
metal nanoparticle
oxidizing agent
Article
cell viability
controlled study
cytochrome c assay
cytokine production
cytotoxicity
cytotoxicity assay
dispersion
high throughput screening
human
human cell
inflammation
nanotoxicology
oxidation reduction potential
oxidative stress
priority journal
protein expression
WST-1 assay
zeta potential
agonists
animal
bronchus
cell line
cell survival
chemical phenomena
chemistry
drug effects
horse
immunology
metabolism
particle size
reproducibility
respiratory mucosa
surface property
toxicity testing
validation study
M3 - Article
PY - 2017
SP - 163-177
ST - Assessment of the oxidative potential of nanoparticles by the cytochrome c
assay: assay improvement and development of a high-throughput method to predict the
toxicity of nanoparticles
T2 - Archives of Toxicology
TI - Assessment of the oxidative potential of nanoparticles by the cytochrome c
assay: assay improvement and development of a high-throughput method to predict the
toxicity of nanoparticles
84964040092&doi=10.1007%2fs00204-016-1701-
3&partnerID=40&md5=f612880c717700ee508761fc487e57f5
VL - 91
ID - 5462
ER -
TY - JOUR
AB - In this study, an adipic acid dihydrazide (ADH)/ tannic acid (TA)-grafted
hyaluronic acid (HA)-based multifunctional hydrogel was synthesized through a
spontaneous amino-yne click reaction and used to promote the improved healing of
infected diabetic wounds. This hydrogel exhibited a range of beneficial properties
such as tunable gelation time, adjustable mechanical properties, pH-sensitive
response characteristics, excellent injectability, the ability to readily adhere to
tissue, and ultra-intimate contact capabilities. Following the encapsulation of
ultrasmall Ag nanoclusters (AgNCs) and deferoxamine loaded polydopamine/ hollow
mesoporous manganese dioxide (PHMD, PDA/H-mMnO2@DFO) nanoparticles, the prepared
hydrogel presented with robust antibacterial, anti-inflammatory, and pro-angiogenic
properties and a desirable smart drug release profile. In this fabricated platform,
PHMD was able to effectively alleviate localized oxidative stress and prolonged
oxygen deprivation via the decomposition of endogenous H2O2 to produce O2. Further
in vivo assays revealed that this hydrogel was capable of facilitating the healing
of infected wounds through the sequential engagement of antibacterial, anti-
inflammatory, and pro-angiogenic activities. Together, this synthesized clickable
environmentally-responsive hydrogel offers great promise as a tool that can be
applied to aid in the healing of chronically infected diabetic wounds and other
inflammatory conditions. © 2022 Elsevier B.V.
AU - Deng, M.
AU - Wu, Y.
AU - Ren, Y.
AU - Song, H.
AU - Zheng, L.
AU - Lin, G.
AU - Wen, X.
AU - Tao, Y.
AU - Kong, Q.
AU - Wang, Y.
DB - Scopus
DO - 10.1016/j.jconrel.2022.08.053
KW - Amino-yne click reaction
Diabetic wound healing
Environmentally-responsive hydrogel
Smart drug delivery
Deferoxamine
Diabetes Mellitus
Humans
Hyaluronic Acid
Hydrogels
Hydrogen Peroxide
Oxygen
Tannins
Amines
Gelation
Hyaluronic acid
Manganese oxide
adipic acid
deferoxamine
hyaluronic acid
hydrogel
hydrogen peroxide
manganese dioxide
nanoparticle
oxygen
silver
tannin
antiinfective agent
tannin derivative
Click reaction
Diabetic wounds
Property
Responsive hydrogels
Smart drug deliveries
Synthesised
Wound healing
angiogenesis
animal cell
animal experiment
animal model
Article
biocompatibility
controlled study
decomposition
diabetic wound
drug release
encapsulation
gelation
human
in vivo study
nonhuman
oxidative stress
oxygenation
rat
synthesis
wound healing
wound infection
diabetes mellitus
M3 - Article
PY - 2022
SP - 613-629
ST - Clickable and smart drug delivery vehicles accelerate the healing of infected
diabetic wounds
TI - Clickable and smart drug delivery vehicles accelerate the healing of infected
diabetic wounds
85137153931&doi=10.1016%2fj.jconrel.2022.08.053&partnerID=40&md5=8976323348ab45c49b
1f8931fc18e11f
VL - 350
ID - 5120
ER -
TY - JOUR
AB - The abuse of antibiotics induces the emergence of drug-resistant bacteria,
which greatly increases the difficulty of clinical treatment of infected wounds. It
is urgent to design a multifunctional wound dressing independent of antibiotics. In
this work, we designed multifunctional hydrogels based on lignin and cellulose in
natural polymers. Lignin with antioxidant properties could reduce silver
nanoparticles in situ and could also be used as a crosslinking agent to construct
hydrogels between hydroxypropyl cellulose modified with phenylboric acid by a
dynamic borate bond. Hydrogels have excellent properties such as self-healing,
shape adaptability, biocompatibility, blood compatibility, antioxidant properties,
excellent broad-spectrum antimicrobial properties, good tissue adhesion, and
electrical conductivity. The tissue adhesion of hydrogels endows them with an
excellent hemostasis property in a rat liver injury model. In vivo experiments
demonstrated that hydrogels can maintain a moist healing environment, reduce
inflammatory cell infiltration, promote M2 macrophage polarization, accelerate
collagen deposition, promote the regeneration of new blood vessels, and
significantly speed up the wound healing of methicillin-resistant Staphylococcus
aureus (MRSA)-infected wounds. Therefore, these multifunctional hydrogels are an
excellent candidate to treat multiple stages of wound healing and have a broad
application prospect in the medical field.
AN - WOS:000718271300050
AU - Deng, P. P.
AU - Chen, F. X.
AU - Zhang, H. D.
AU - Chen, Y.
AU - Zhou, J. P.
C6 - NOV 2021
DA - NOV 10
DO - 10.1021/acsami.1c14608
IS - 44
PY - 2021
SN - 1944-8244
1944-8252
SP - 52333-52345
ST - Conductive, Self-Healing, Adhesive, and Antibacterial Hydrogels Based on
Lignin/Cellulose for Rapid MRSA-Infected Wound Repairing
T2 - ACS APPLIED MATERIALS & INTERFACES
TI - Conductive, Self-Healing, Adhesive, and Antibacterial Hydrogels Based on
Lignin/Cellulose for Rapid MRSA-Infected Wound Repairing
VL - 13
ID - 6492
ER -
TY - JOUR
AB - The abuse of antibiotics induces the emergence of drug-resistant bacteria,
which greatly increases the difficulty of clinical treatment of infected wounds. It
is urgent to design a multifunctional wound dressing independent of antibiotics. In
this work, we designed multifunctional hydrogels based on lignin and cellulose in
natural polymers. Lignin with antioxidant properties could reduce silver
nanoparticles in situ and could also be used as a crosslinking agent to construct
hydrogels between hydroxypropyl cellulose modified with phenylboric acid by a
dynamic borate bond. Hydrogels have excellent properties such as self-healing,
shape adaptability, biocompatibility, blood compatibility, antioxidant properties,
excellent broad-spectrum antimicrobial properties, good tissue adhesion, and
electrical conductivity. The tissue adhesion of hydrogels endows them with an
excellent hemostasis property in a rat liver injury model. In vivo experiments
demonstrated that hydrogels can maintain a moist healing environment, reduce
inflammatory cell infiltration, promote M2 macrophage polarization, accelerate
collagen deposition, promote the regeneration of new blood vessels, and
significantly speed up the wound healing of methicillin-resistant Staphylococcus
aureus (MRSA)-infected wounds. Therefore, these multifunctional hydrogels are an
excellent candidate to treat multiple stages of wound healing and have a broad
application prospect in the medical field. © 2021 American Chemical Society. All
rights reserved.
AU - Deng, P.
AU - Chen, F.
AU - Zhang, H.
AU - Chen, Y.
AU - Zhou, J.
DB - Scopus
DO - 10.1021/acsami.1c14608
IS - 44
KW - Ag-lignin nps
Conductive
Hydrogel
Hydroxypropyl cellulose
Wound healing
Adhesion
Adhesives
Antibiotics
Antioxidants
Bacteria
Blood
Biocompatibility
Blood vessels
Cellulose
Drug delivery
Free radicals
Lignin
Metal nanoparticles
Self-healing materials
Tissue
Tissue regeneration
Ag-lignin NP
Infected wounds
Methicillin-resistant staphylococcus aureus
Property
Self-healing
Tissue adhesion
Hydrogels
M3 - Article
PY - 2021
SP - 52333-52345
ST - Conductive, self-healing, adhesive, and antibacterial hydrogels based on
lignin/cellulose for rapid mrsa-infected wound repairing
T2 - ACS Applied Materials and Interfaces
TI - Conductive, self-healing, adhesive, and antibacterial hydrogels based on
lignin/cellulose for rapid mrsa-infected wound repairing
85119431888&doi=10.1021%2facsami.1c14608&partnerID=40&md5=7716949bb677ca3a800cc3c16
aef7bfd
VL - 13
ID - 5182
ER -
TY - JOUR
AB - Solar burn reactivation, a rare and idiosyncratic drug reaction, has been
reported with the use of a variety of drugs. This reaction is believed to be the
result of exposure to ultraviolet light during the subsiding phase of an acute
inflammatory reaction. It affects areas of the body that have been previously
sunburned. We describe a 16-year-old girl who was receiving treatment for acute
lymphoblastic leukemia and experienced a second-degree solar burn reactivation
reaction to methotrexate. The patient had a mild sunburn on her face and shoulders
the day she went to the oncology clinic for her interim maintenance chemotherapy
with vincristine 1.5 mg/m2/dose and methotrexate 100 mg/m2/dose. Three days later,
she returned to the clinic with a 2-day history of fever (≤ 100.2°F), nausea,
vomiting, and malaise; the sunburn on her face and shoulders also had become
severe, without further sun exposure. Laboratory results revealed elevated blood
urea nitrogen and serum creatinine concentrations, and her methotrexate level was
elevated at 0.9 mM. The patient was diagnosed with acute renal failure,
dehydration, methotrexate toxicity, and second-degree solar burn reactivation
reaction. She was admitted to the children's hospital and treated with sodium
bicarbonate, acetaminophen with codeine, ondansetron, and silvadene cream. On
hospital day 3, the patient's methotrexate level decreased to less than 0.1 mM. The
sunburn continued to heal, and after a 14-day hospital stay, complicated by a
streptococcal infection, grade 3 mucositis, bacteremia, and mild gastritis and
duodenitis, the patient recovered and was discharged. Use of the Naranjo adverse
drug reaction probability scale indicated a probable relationship (score of 6)
between the patient's solar burn reactivation and methotrexate. Although
methotrexate-induced solar burn reactivation is rare, clinicians should be aware of
this potential adverse reaction and consider delaying administration of
methotrexate by 5-7 days if a patient reports ultraviolet-related erythema in the
past 2-4 days or presents with a notable sunburn.
AU - DeVore, K. J.
DB - Scopus
DO - 10.1592/phco.30.4.419
IS - 4
KW - Chemotherapy
Dermatology
Methotrexate
Photoreaction
Photosensitivity
Solar burn reactivation
Sunburn
Toxicity
Acute Disease
Adolescent
Antineoplastic Agents, Phytogenic
Blood Urea Nitrogen
Creatinine
Female
Humans
Kidney Failure, Acute
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Treatment Outcome
Ultraviolet Rays
Vincristine
bicarbonate
codeine
creatinine
methotrexate
ondansetron
paracetamol
sulfadiazine silver
vincristine
acute kidney failure
acute lymphoblastic leukemia
adolescent
article
bacteremia
cancer patient
case report
convalescence
cream
creatinine blood level
dehydration
disease severity
duodenitis
erythema
face
female
fever
gastritis
healing
hospital discharge
human
length of stay
maintenance therapy
malaise
mucosa inflammation
Naranjo adverse drug reaction probability scale
nausea
rating scale
shoulder
side effect
Streptococcus infection
sun exposure
sunburn
vomiting
M3 - Article
PY - 2010
SP - 123e-126e
ST - Solar burn reactivation induced by methotrexate
T2 - Pharmacotherapy
TI - Solar burn reactivation induced by methotrexate
77950455599&doi=10.1592%2fphco.30.4.419&partnerID=40&md5=4efb1b355d626781ea01b9addb
c04d22
VL - 30
ID - 5664
ER -
TY - JOUR
AB - Green nanotechnology elucidates highly prioritized anticancer activity. We
synthesized Copper oxide nanoparticles (CuONPs) using leaves of Azadirachta indica
(A. indica) plants and studied the molecular mechanism of cancer cell apoptosis.
After their synthesis, with the help of expository tools like Fourier transform
infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Dynamic
light scattering (DLS) and surface zeta potential we confirmed the successful
synthesis of CuONPs. Here, crystalline structure of green synthesized CuONPs of 36
+/- 8 nm size and spherical shape was able to kill MCF-7 and Hela cells, estimated
by MTT assay. Successful internalization of Cu+2 ions inside the cell was estimated
by the atomic absorption study. Cellular uptake of Cu+2 ions inflicted significant
Reactive Oxygen Species (ROS) generation inside the cancer cells, thereby leading
to DNA fragmentation as observed by DAPI staining. In in vivo model, CuONPs reduced
the breast tumor volume in Balb/C mice and increased the mean survival time through
the alteration of pro-inflammatory cytokines level. In case of both in vivo and in
vitro models, CuONPs altered the pro-inflammatory cytokine level and pro-apoptotic
protein expressions. In future, green synthesized CuONPs might be beneficial for
its application as an anticancer drug in in vivo (mice model) and in vitro, though
further study is needed on its toxicity. (C) 2018 King Saud University. Production
and hosting by Elsevier B.V.
AN - WOS:000456445700012
AU - Dey, A.
AU - Manna, S.
AU - Chattopadhyay, S.
AU - Mondal, D.
AU - Chattopadhyay, D.
AU - Raj, A.
AU - Das, S.
AU - Bag, B. G.
AU - Roy, S.
DA - FEB
DO - 10.1016/j.jscs.2018.06.011
IS - 2
PY - 2019
SN - 1319-6103
2212-4640
SP - 222-238
ST - Azadirachta indica leaves mediated green synthesized copper oxide
nanoparticles induce apoptosis through activation of TNF-alpha and caspases
signaling pathway against cancer cells
T2 - JOURNAL OF SAUDI CHEMICAL SOCIETY
TI - Azadirachta indica leaves mediated green synthesized copper oxide
nanoparticles induce apoptosis through activation of TNF-alpha and caspases
signaling pathway against cancer cells
VL - 23
ID - 6348
ER -
TY - JOUR
AB - With the increasing focus on healthcare research in the current times,
therapeutic and biomaterial interventions for healing of wounds and mitigation of
wound-associated infections have seen expedited progress. Conventional approaches
consist of release-active gels, which demonstrate leaching of antimicrobials, such
as antibiotics, metal ions, etc. However, these systems suffer from the
disadvantages of burst release, reservoir exhaustion, and associated toxicity. In
this report, intrinsically antimicrobial hydrogel (HyDex) is developed by one-pot
UV crosslinking of methacrylated dextran, polyethylene glycol diacrylate, and
cationic lipophilic methacrylate with varied hydrophobic chain, which displays
broad-spectrum antimicrobial activity, hemostatic ability, and rapid wound closure
efficacy. The optimized hydrogel exhibits potent antimicrobial efficacy against
multidrug-resistant Gram-positive and Gram-negative bacteria as well as against
pathogenic fungus Candida albicans. The HyDex hydrogel shows rapid arrest of
bleeding in mice liver puncture model. The hydrogel kills carbapenem-resistant
Acinetobacter baumannii in a murine model of burn wound infection with >99%
reduction in bacterial burden. Furthermore, this hydrogel displays significant
reduction in inflammatory responses, with accelerated wound healing in rat deep
wound model. Collectively, these results imply the excellent promise held by lead
hydrogel to be developed for tackling deep tissue wounds, notorious infections, and
resulting inflammatory responses.
AN - WOS:000812698700001
AU - Dey, R.
AU - Mukherjee, R.
AU - Haldar, J.
C6 - JUN 2022
C7 - 2200536
DA - AUG
DO - 10.1002/adhm.202200536
IS - 15
PY - 2022
SN - 2192-2640
2192-2659
ST - Photo-Crosslinked Antimicrobial Hydrogel Exhibiting Wound Healing Ability and
Curing Infections In Vivo
TI - Photo-Crosslinked Antimicrobial Hydrogel Exhibiting Wound Healing Ability and
Curing Infections In Vivo
VL - 11
ID - 6740
ER -
TY - JOUR
AB - Various studies have shown that the reproductive organs are highly sensitive
to toxic elements found in the environment. Due to technological progress, the use
of nanoparticles has become more common nowadays. Nanoparticles are used for drug
delivery because their dimensions allow them to circulate throughout the body and
enter directly into the cell. Antimicrobial properties are increasingly used in the
manufacture of medical devices, textiles, food packaging, cosmetics, and other
consumer products. Nanoparticles provide several benefits, but aspects related to
their effects on living organisms and the environment are not well known. This
review summarizes current in vivo, and in vitro animal studies focused on the
evaluation of toxicity of selected metal nanoparticles (Ag, ZnO, TiO2) on male and
female reproductive health. It can be concluded that higher concentrations of metal
nanoparticles in the male reproductive system can cause a decrease in spermatozoa
motility, viability and disruption of membrane integrity. Histopathological changes
of the testicular epithelium, infiltration of inflammatory cells in the epididymis,
and prostatic hyperplasia have been observed. Nanoparticles in the female
reproductive system caused their accumulation in the ovaries and uterus. Metal
nanoparticles most likely induce polycystic ovary syndrome and follicular atresia,
inflammation, apoptosis, and necrosis also occurred. © 2022 by the authors.
AU - Dianová, L.
AU - Tirpák, F.
AU - Halo, M.
AU - Slanina, T.
AU - Massányi, M.
AU - Stawarz, R.
AU - Formicki, G.
AU - Madeddu, R.
AU - Massányi, P.
C7 - 459
DB - Scopus
DO - 10.3390/toxics10080459
IS - 8
KW - Ag
nanoparticles
reproductive system
TiO2
toxicity
ZnO
silver nanoparticle
apoptosis
epididymis
female genital system
health hazard
histopathology
human
in vitro study
in vivo study
inflammation
inflammatory cell
male genital system
membrane damage
necrosis
ovary follicle atresia
ovary polycystic disease
oxidative stress
prostate hypertrophy
reproductive health
Review
sperm viability
spermatozoon motility
M3 - Review
PY - 2022
ST - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
Function of Reproductive Organs
T2 - Toxics
TI - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
85137353498&doi=10.3390%2ftoxics10080459&partnerID=40&md5=66b28ec2158f21e2005fe5d7d
b010943
VL - 10
ID - 5112
ER -
TY - JOUR
AB - Various studies have shown that the reproductive organs are highly sensitive
to toxic elements found in the environment. Due to technological progress, the use
of nanoparticles has become more common nowadays. Nanoparticles are used for drug
delivery because their dimensions allow them to circulate throughout the body and
enter directly into the cell. Antimicrobial properties are increasingly used in the
manufacture of medical devices, textiles, food packaging, cosmetics, and other
consumer products. Nanoparticles provide several benefits, but aspects related to
their effects on living organisms and the environment are not well known. This
review summarizes current in vivo, and in vitro animal studies focused on the
evaluation of toxicity of selected metal nanoparticles (Ag, ZnO, TiO2) on male and
female reproductive health. It can be concluded that higher concentrations of metal
nanoparticles in the male reproductive system can cause a decrease in spermatozoa
motility, viability and disruption of membrane integrity. Histopathological changes
of the testicular epithelium, infiltration of inflammatory cells in the epididymis,
and prostatic hyperplasia have been observed. Nanoparticles in the female
reproductive system caused their accumulation in the ovaries and uterus. Metal
nanoparticles most likely induce polycystic ovary syndrome and follicular atresia,
inflammation, apoptosis, and necrosis also occurred.
AN - WOS:000845115300001
AU - Dianova, L.
AU - Tirpak, F.
AU - Halo, M.
AU - Slanina, T.
AU - Massanyi, M.
AU - Stawarz, R.
AU - Formicki, G.
AU - Madeddu, R.
AU - Massanyi, P.
C7 - 459
DA - AUG
DO - 10.3390/toxics10080459
IS - 8
PY - 2022
SN - 2305-6304
ST - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
T2 - TOXICS
TI - Effects of Selected Metal Nanoparticles (Ag, ZnO, TiO2) on the Structure and
VL - 10
ID - 6527
ER -
TY - JOUR
AB - The N-heterocyclic carbene (NHC) precursor 2-pyridin-2-yl-2H-imidazo[1,5-
a]pyridin-4-ylium hexafluorophosphate (1 center dot HPF6) was used to synthesize
various Ag and Au complexes, including [Ag(1)(2)][PF6] (2), [Au(1)(2)][PF6] (3) and
[Au(1)Cl-3] (4). The structure of the silver(I) complex 2 was established via NMR
spectroscopy, mass spectrometry and single crystal X-ray crystallography. The
gold(I)-NHC complex 3 was synthesized via transmetallation of the aforementioned
silver complex and characterized using various spectroscopic methods. Treatment of
3 with Au(SMe2)Cl afforded 4, ostensibly via a disproportionation process. Close
inspection of the solid state structure of 2 revealed that the Ag(I) center adopted
a linear geometry; in contrast, a square planar geometry was observed for the solid
structure of 4. The cytotoxicities of the gold complexes 3 and 4 were tested in
vitro against Human colorectal carcinoma (HCT 116), Human hepatocellular carcinoma
(HepG2), Human breast adenocarcinoma (MCF-7) and Murine melanoma (B16F10). The
measured IC50 values showed that the Au(I) complex 3 was more potent than the
Au(III) complex 4 as well as cisplatin.
AN - WOS:000331805000046
AU - Dinda, J.
AU - Samanta, T.
AU - Nandy, A.
AU - Das Saha, K.
AU - Seth, S. K.
AU - Chattopadhyay, S. K.
AU - Bielawskie, C. W.
DO - 10.1039/c3nj01463k
IS - 3
PY - 2014
SN - 1144-0546
1369-9261
SP - 1218-1224
ST - N-heterocyclic carbene supported Au(I) and Au(III) complexes: a comparison of
cytotoxicities
T2 - NEW JOURNAL OF CHEMISTRY
TI - N-heterocyclic carbene supported Au(I) and Au(III) complexes: a comparison of
cytotoxicities
VL - 38
ID - 6835
ER -
TY - JOUR
AB - The current study was designed to investigate the cytotoxicity and
immunomodulatory effects of sol-gel combustion based TiO2 particles (glycine and L-
alanine as reducing agents) of large surface area on RAW 264.7 macrophages. RAW
264.7 macrophages exposed to varying concentrations of TiO2 particles (0.001 to
1000 mu g/ml) were assessed after 24 h and showed a reduced cell viability at 100
and 1000 mu g/ml and increased LDH release at 10 mu g/ml. Furthermore, TiO2
particles (0.1, 1 and 10 mu g/ml) were utilized to assess the immune responses and
intracellular ROS levels on RAW 264.7 macrophages. TiO2 particles at 10 mu g/ml
showed increased mRNA expression of inflammatory cytokines (TNF alpha, IL-1 beta
and IL-6), inflammatory mediators (iNOS and COX -2) and transcription factor (NFO)
similar to that of LPS stimulated macrophages. However, the mRNA expression levels
were found near normal levels at lower concentrations (0.1 and 1 mu g/ml). In
addition, TiO2 particles at 10 mu g/ml also increased the production of
inflammatory cytokines (TNF alpha, IL-1 beta and IL-6) and intracellular ROS levels
in RAW 264.7 macrophages similar to that of LPS stimulated macrophages.
Conclusively, TiO2 particles prepared through this method at a concentration <= 0.1
mu g/ml can be used for various biological applications with minimal
immunomodulatory effects.
AN - WOS:000405157200013
AU - Dinesh, P.
AU - Yadav, C. S.
AU - Kannadasan, S.
AU - Rasool, M.
DA - SEP
DO - 10.1016/j.tiv.2017.06.006
PY - 2017
SN - 0887-2333
SP - 92-103
ST - Cytotoxicity and immunomodulatory effects of sol-gel combustion based
titanium dioxide (TiO2) particles of large surface area on RAW 264.7 macrophages
TI - Cytotoxicity and immunomodulatory effects of sol-gel combustion based
titanium dioxide (TiO2) particles of large surface area on RAW 264.7 macrophages
VL - 43
ID - 6450
ER -
TY - JOUR
AB - The purpose of this study was to develop a promising wound dressing. Though
chitosan cross-linked with genipin has been widely used as biomaterials, with the
addition of partially oxidized Bletilla striata polysaccharide, the newly developed
material in this study (coded as CSGB) showed less gelling time, more uniform
aperture distribution, higher water retention, demanded mechanical strength and
more L929 cell proliferation compared to the chitosan cross-linked only with
genipin. Owning to partial blocking of free amino groups of chitosan, CSGB revealed
almost no antibacterial activities, thus the bilayer composite of chitosan-silver
nanoparticles (CS-AgG) on CSGB was designed to inhibit microbial invasion. The in
vivo studies indicated that both CSGB and bilayer wound dressing significantly
accelerated the healing rate of cutaneous wounds in mice, and the bilayer exhibited
better mature epidermization with less inflammatory cells on Day 7. Therefore, this
novel bilayer composite has great potential in wound dressing applications. (C)
2016 Elsevier Ltd. All rights reserved.
AN - WOS:000391896800172
AU - Ding, L.
AU - Shan, X. D.
AU - Zhao, X. L.
AU - Zha, H. L.
AU - Chen, X. Y.
AU - Wang, J. J.
AU - Cai, C.
AU - Wang, X. J.
AU - Li, G. Y.
AU - Hao, J. J.
AU - Yu, G. L.
DA - FEB 10
DO - 10.1016/j.carbpol.2016.11.040
PY - 2017
SN - 0144-8617
1879-1344
SP - 1538-1547
ST - Spongy bilayer dressing composed of chitosan-Ag nanoparticles and chitosan-
Bletilla striata polysaccharide for wound healing applications
TI - Spongy bilayer dressing composed of chitosan-Ag nanoparticles and chitosan-
Bletilla striata polysaccharide for wound healing applications
VL - 157
ID - 6259
ER -
TY - JOUR
AB - PurposePeri-implantitis is one of the most common inflammatory complications
in dental implantology. Similar to periodontitis, in peri-implantitis, destructive
inflammatory changes take place in the tissues surrounding a dental implant.
Bacterial flora at the failing implant sites resemble the pathogens in periodontal
disease and consist of Gram-negative anaerobic bacteria including Aggregatibacter
actinomycetemcomitans (Aa). Here we demonstrate the effectiveness of a silver
lactate (SL)-containing RGD-coupled alginate hydrogel scaffold as a promising stem
cell delivery vehicle with antimicrobial properties. Materials and MethodsGingival
mesenchymal stem cells (GMSCs) or human bone marrow mesenchymal stem cells
(hBMMSCs) were encapsulated in SL-loaded alginate hydrogel microspheres. Stem cell
viability, proliferation, and osteo-differentiation capacity were analyzed.
ResultsOur results showed that SL exhibited antimicrobial properties against Aa in
a dose-dependent manner, with 0.50 mg/ml showing the greatest antimicrobial
properties while still maintaining cell viability. At this concentration, SL-
containing alginate hydrogel was able to inhibit Aa growth on the surface of Ti
discs and significantly reduce the bacterial load in Aa suspensions. Silver ions
were effectively released from the SL-loaded alginate microspheres for up to 2
weeks. Osteogenic differentiation of GMSCs and hBMMSCs encapsulated in the SL-
loaded alginate microspheres were confirmed by the intense mineral matrix
deposition and high expression of osteogenesis-related genes. ConclusionTaken
together, our findings confirm that GMSCs encapsulated in RGD-modified alginate
hydrogel containing SL show promise for bone tissue engineering with antimicrobial
properties against Aa bacteria in vitro.
AN - WOS:000371426300003
AU - Diniz, I. M. A.
AU - Chen, C.
AU - Ansari, S.
AU - Zadeh, H. H.
AU - Moshaverinia, M.
AU - Chee, D.
AU - Marques, M. M.
AU - Shi, S. T.
AU - Moshaverinia, A.
DA - FEB
DO - 10.1111/jopr.12316
IS - 2
PY - 2016
SN - 1059-941X
1532-849X
SP - 105-115
ST - Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD-Coupled
Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for
Peri-Implantitis
T2 - JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY
TI - Gingival Mesenchymal Stem Cell (GMSC) Delivery System Based on RGD-Coupled
Alginate Hydrogel with Antimicrobial Properties: A Novel Treatment Modality for
Peri-Implantitis
VL - 25
ID - 6313
ER -
TY - JOUR
AB - Burn wound progression is an inflammation-driven process where an initial
partial-thickness thermal burn wound can evolve over time to a full-thickness
injury. We have developed an oil-in-water nanoemulsion formulation (NB-201)
containing benzalkonium chloride for use in burn wounds that is antimicrobial and
potentially inhibits burn wound progression. We used a porcine burn injury model to
evaluate the effect of topical nanoemulsion treatment on burn wound conversion and
healing. Anesthetized swine received thermal burn wounds using a 25-cm(2) surface
area copper bar heated to 80 degrees C. Three different concentrations of NB-201
(10, 20, or 40% nanoemulsion), silver sulfadiazine cream, or saline were applied to
burned skin immediately after injury and on days 1, 2, 4, 7, 10, 14, and 18
postinjury. Digital images and skin biopsies were taken at each dressing change.
Skin biopsy samples were stained for histological evaluation and graded. Skin
tissue samples were also assayed for mediators of inflammation. Dermal treatment
with NB-201 diminished thermal burn wound conversion to a full-thickness injury as
determined by both histological and visual evaluation. Comparison of epithelial
restoration on day 21 showed that 77.8% of the nanoemulsion-treated wounds had an
epidermal injury score of 0 compared to 16.7% of the silver sulfadiazine-treated
burns (P = .01). Silver sulfadiazine cream- and saline-treated wounds (controls)
converted to full-thickness burns by day 4. Histological evaluation revealed
reduced inflammation and evidence of skin injury in NB-201-treated sites compared
to control wounds. The nanoemulsion-treated wounds often healed with complete
regrowth of epithelium and no loss of hair follicles (NB-201: 4.8 +/- 2.1, saline:
0 +/- 0, silver sulfadiazine: 0 +/- 0 hair follicles per 4-mm biopsy section, P
< .05). Production of inflammatory mediators and sequestration of neutrophils were
also inhibited by NB-201. Topically applied NB-201 prevented the progression of a
partial-thickness burn wound to full-thickness injury and was associated with a
concurrent decrease in dermal inflammation.
AN - WOS:000728187700025
AU - Dolgachev, V. A.
AU - Ciotti, S.
AU - Liechty, E.
AU - Levi, B.
AU - Wang, S. C.
AU - Baker, Jr.
AU - Hemmila, M. R.
C6 - JUN 2021
DA - NOV-DEC
DO - 10.1093/jbcr/irab118
IS - 6
PY - 2021
SN - 1559-047X
1559-0488
SP - 1232-1242
ST - Dermal Nanoemulsion Treatment Reduces Burn Wound Conversion and Improves Skin
Healing in a Porcine Model of Thermal Burn Injury
T2 - JOURNAL OF BURN CARE & RESEARCH
TI - Dermal Nanoemulsion Treatment Reduces Burn Wound Conversion and Improves Skin
Healing in a Porcine Model of Thermal Burn Injury
VL - 42
ID - 6781
ER -
TY - JOUR
AB - Here we describe development of a silicone rubber/stainless steel mesh cage
implant system, much like that used to assess biocompatibility of biomaterials [1],
for easy removal of injectable polymer micro spheres in vivo. The sterile cage has
a type 316 stainless steel mesh size (38 gm) large enough for cell penetration and
free fluid flow in vivo but small enough for microsphere retention, and a silicone
rubber shell for injection of the microspheres. Two model drugs, the poorly soluble
steroid, triamcinolone acetonide, and the highly water-soluble luteinizing hormone-
releasing hormone (LHRH) peptide superagonist, leuprolide, were encapsulated in
PLGA microspheres large enough (63-90 mu m) to be restrained' by the cage implant
in vivo. The in vitro release from both formulations was followed by ultra
performance liquid chromatography (UPLC) with and without the cage in a standard
release media, PBS pH 7.4 + 0.02% Tween 80 0.05% sodium azide, at 37 degrees C.
Pharmacokinetics (PM) in rats was assessed after SC injection or SC in-cage
implantation of microspheres with plasma analysis by LC-MS/MS or EIA. Tr-A and
leuprolide in vitro release was largely unaffected after the initial, burst
irrespective of the cage or test tube incubation vessel and release was much slower
than observed in vivo for both drugs. Moreover, Tr-A and leuprolide
pharmacokinetics with and without the cage were highly similar during the 2-3 week
release duration before a significant inflammatory response was caused by the cage
implant. Hence, the PM-validated cage implant provides a simple means to recover
and evaluate the microsphere drug carriers in vivo during a time window of at least
a few weeks in order to characterize the polymer microsphere release and erosion
behavior in vivo. This approach may facilitate development of mechanism-based in
vitro/in vivo correlations and enable development of more accurate and useful in
vitro release tests. (C) 2016 Elsevier Ltd. All rights reserved.
AN - WOS:000386407500009
AU - Doty, A. C.
AU - Hirota, K.
AU - Olsen, K. F.
AU - Sakamoto, N.
AU - Ackermann, R.
AU - Feng, M. R.
AU - Wang, Y.
AU - Choi, S.
AU - Qu, W.
AU - Schwendeman, A.
AU - Schwendeman, S. P.
DA - DEC
DO - 10.1016/j.biomaterials.2016.07.041
PY - 2016
SN - 0142-9612
1878-5905
SP - 88-96
ST - Validation of a cage implant system for assessing in vivo performance of
long-acting, release microspheres
T2 - BIOMATERIALS
TI - Validation of a cage implant system for assessing in vivo performance of
long-acting, release microspheres
VL - 109
ID - 6813
ER -
TY - JOUR
AB - The use of selective barriers as resorbable membranes has become a routine
clinical procedure for guided bone regeneration. Therefore, the production of
membranes with a low inflammatory potential during their resorption process has
become the goal of a considerable number of researches. Aim: The purpose of the
present study was to evaluate the biocompatibility of poly (L- lactic acid) (PLLA)
and biocelulose membranes (BC) inserted in the subcutaneous tissue on the dorsum of
rats. Methods: Fifteen animals underwent surgical procedures for the insertion of 4
types of membranes: COL (Collagen membrane) Control Group; BC (Biocellulose
membrane); BCAg (Biocellulose membrane impregnated with Silver); PLLA (Poly (L-
lactic acid) membrane). All membrane types were inserted into each animal. Animals
were euthanized after 3, 7, and 15 days of the surgical procedure. Descriptive
histological analyses were carried out to investigate host tissue reaction to
membrane presence by assessing the anti-inflammatory process composition associated
with the membrane resorption and the presence of foreign-body reaction or
encapsulation. Results: The BC membranes showed a higher degree of inflammation and
poor pattern of integration with the surrounding tissues than the PLLA and COL
membranes. Conclusion: The PLLA and COL membranes present better biocompatibility
than the BC membranes
AD - Doval Neto, José
Araraquara University Center. Araraquara Dental School. Department of Postgraduate
Studies in Implantology. Araraquara. BR
Marques, Rodrigo Fernando Costa
Paulista State University. Institute of Chemistry. Araraquara. BR
Motta, Adriana Cristina
Pontifical Catholic University of São Paulo. Faculty of Medical Sciences and
Health. Biomaterials Laboratory. Sorocaba. BR
Duek, Eliana Aparecida de Rezende
Pontifical Catholic University of São Paulo. Faculty of Medical Sciences and
Health. Biomaterials Laboratory. Sorocaba. BR
Oliveira, Guilherme José Pimentel Lopes de
Federal University of Uberlândia. School of Dentistry. Department of
Periodontology/Implantodontology. Uberlândia. BR
Marcantonio, Cláudio
Araraquara University Center. Araraquara Dental School. Department of Postgraduate
Studies in Implantology. Araraquara. BR
AU - Doval Neto, José
AU - Marques, Rodrigo Fernando Costa
AU - Motta, Adriana Cristina
AU - Duek, Eliana Aparecida de Rezende
AU - Oliveira, Guilherme José Pimentel Lopes de
AU - Marcantonio, Cláudio
C1 - 20220920
DA - 2022/12
DB - LILACS
DO - 10.20396/bjos.v21i00.8670616
KW - Biocompatible materials
Celulose
Inflammation
Membranes
Polyesters
LA - en
PY - 2022
SN - 1677-3217
SP - e220616-e220616
ST - Analysis of the biocompatibility of a biocelulose and a poly L- lactic acid
membrane
T2 - Braz. j. oral sci
TI - Analysis of the biocompatibility of a biocelulose and a poly L- lactic acid
membrane
UR - https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/
8670616/29880
VL - 21
ID - 4916
ER -
TY - JOUR
AB - This work describes measurement methodology on acrylic acid-based hydrogels
with nanogold and starch, including evaluation of pro-inflammatory activity and
cytotoxicity by MIT reduction assay, swelling capacity, tensile strength using
Brookfield texture analyzer, surface morphology by scanning electron microscopy,
structure by FT-IR spectroscopy, crystallinity by X-ray diffraction and hydrogel
behaviour in simulated body fluids. The modified polymers exhibited approx. 75%
lower deformation than unmodified polymer. Hydrogels showed buffering properties in
simulated body fluids. Hydrogels containing 5-10% starch solution did not exhibit
cytotoxicity. Cells exposed to unmodified hydrogel and to hydrogels containing 5%
and 10% starch solutions were characterized by the desired morphology. Importantly,
hydrogel nanocomposites have not showed pro-inflammatory activity. It was proved
that highly developed specific surface area of modified hydrogels affected their
sorption properties. Nanogold present in nanocomposites did not affect directly
their physicochemical properties but, due to its features, antibacterial activity
of so modified materials could be enhanced. (C) 2020 The Author(s). Published by
Elsevier Ltd.
AN - WOS:000519983300040
AU - Drabczyk, A.
AU - Kudlacik-Kramarczyk, S.
AU - Tyliszczak, B.
AU - Rudnicka, K.
AU - Urbaniak, M.
AU - Michlewska, S.
AU - Krolczyk, J. B.
AU - Gajda, P.
AU - Pielichowski, K.
C7 - 107608
DA - MAY
DO - 10.1016/j.measurement.2020.107608
PY - 2020
SN - 0263-2241
1873-412X
ST - Measurement methodology toward determination of structure-property
relationships in acrylic hydrogels with starch and nanogold designed for biomedical
applications
T2 - MEASUREMENT
TI - Measurement methodology toward determination of structure-property
relationships in acrylic hydrogels with starch and nanogold designed for biomedical
applications
VL - 156
ID - 6627
ER -
TY - JOUR
AB - The most important concept behind using bone scaffolds is the
biocompatibility of the material to avoid a local inflammatory response and must
have the following properties: osteoinduction, osteoconductivity, angiogenesis, and
mechanical support for cell growth. Gold nanoparticles/gold and silver
nanoparticles -containing bioactive glasses in biopolymer composites have been used
to enhance bone regeneration. These composites were tested in vitro on fibroblast
and osteoblast cell lines using MTT tests, immunofluorescence, scanning electron
microscopy analysis, and in vivo in an experimental bone defect in Sprague-Dawley
rats. Both composites promoted adequate biological effects on human fibroblastic BJ
(CRL 2522TM) cell lines and human osteoblastic cells isolated from the human
patella in terms of cell proliferation, morphology, migration, and attachment. Most
importantly, they did not cause cellular apoptosis and necrosis. According to the
histological and immunohistochemical results, both composites were osteoinductive
and promoted new bone formation at 60 d. Evidence from this study suggests that the
small amount of silver content does not influence negatively the in vitro or in
vivo results. In addition, we obtained accurate results proving that the existence
of apatite layer and proteins on the surface of the recovered composite, supports
the validity of in vitro bioactivity research. © 2023 IOP Publishing Ltd.
AU - Dreanca, A.
AU - Bogdan, S.
AU - Popescu, A.
AU - Sand, D.
AU - Pall, E.
AU - Astilean, A. N.
AU - Pestean, C.
AU - Toma, C.
AU - Marza, S.
AU - Taulescu, M.
AU - Cenariu, M.
AU - Sevastre, B.
AU - Oana, L.
AU - Todea, M.
AU - Baia, L.
AU - Magyari, K.
C7 - 055014
DB - Scopus
DO - 10.1088/1748-605X/ace9a6
IS - 5
KW - alginate
bone regeneration biomarker
cell attachment
immunofluorescence
pullulan
Animals
Biopolymers
Bone Regeneration
Gold
Humans
Metal Nanoparticles
Rats
Silver
Tissue Scaffolds
Bioactive glass
Biocompatibility
Biomolecules
Bone
Cell culture
Cell proliferation
Fluorescence
Gold nanoparticles
Metal nanoparticles
Morphology
Phosphate minerals
Scaffolds (biology)
alginic acid
apatite
biopolymer
enrofloxacin
glass
gold nanoparticle
isoflurane
ketamine
oxygen
protein
silver nanoparticle
tramadol
xylazine
gold
metal nanoparticle
silver
Bone defect
Bone regeneration
Bone regeneration biomarker
Bone scaffolds
Cell attachments
Immunofluorescence
In-vitro
In-vivo
Pullulans
animal experiment
animal model
animal tissue
apoptosis
apoptosis assay
Article
biocompatibility
biological activity
BJ [Human fibroblast] cell line
bone defect
bone regeneration
bone tissue
cell adhesion
cell migration
cell proliferation
cell structure
cell viability
controlled study
evaluation study
femur diaphysis
fibroblast cell line
histopathology
human
human cell
in vitro study
in vivo study
male
MTT assay
necrosis
necrosis assay
nonhuman
ossification
osteoblast cell line
patella
rat
Sprague Dawley rat
surgical technique
validity
X ray diffraction
animal
chemistry
Cell death
M3 - Article
PY - 2023
ST - The evaluation of the osteopromoting capabilities of composites based on
biopolymers and gold/silver nanoparticles doped bioactive glasses on an
experimental rat bone defect
TI - The evaluation of the osteopromoting capabilities of composites based on
biopolymers and gold/silver nanoparticles doped bioactive glasses on an
experimental rat bone defect
85166442745&doi=10.1088%2f1748-605X
%2face9a6&partnerID=40&md5=8322612fc8ef9056d91901c1fcb1898a
VL - 18
ID - 4992
ER -
TY - JOUR
AB - Antimicrobial resistance poses a significant threat to public health and
social development worldwide. This study aimed to investigate the effectiveness of
silver nanoparticles (AgNPs) in treating multidrug-resistant bacterial infections.
Eco-friendly spherical AgNPs were synthesized using rutin at room temperature. The
biocompatibility of both polyvinyl pyrrolidone (PVP) and mouse serum (MS)-
stabilized AgNPs was evaluated at 20 μg/mL and showed a similar distribution in
mice. However, only MS-AgNPs significantly protected mice from sepsis caused by the
multidrug-resistant Escherichia coli (E. coli) CQ10 strain (p = 0.039). The data
revealed that MS-AgNPs facilitated the elimination of Escherichia coli (E. coli) in
the blood and the spleen, and the mice experienced only a mild inflammatory
response, as interleukin-6, tumor necrosis factor-α, chemokine KC, and C-reactive
protein levels were significantly lower than those in the control group. The
results suggest that the plasma protein corona strengthens the antibacterial effect
of AgNPs in vivo and may be a potential strategy for combating antimicrobial
resistance. Copyright © 2023 Du, Wang, Zhang, Chen, Deng, He, Tang, Deng and Ren.
AU - Du, H.
AU - Wang, X.
AU - Zhang, H.
AU - Chen, H.
AU - Deng, X.
AU - He, Y.
AU - Tang, H.
AU - Deng, F.
AU - Ren, Z.
C7 - 1153147
DB - Scopus
DO - 10.3389/fmicb.2023.1153147
KW - multidrug-resistant bacteria
pro-inflammatory cytokine
protein corona
sepsis
C reactive protein
creatinine
interleukin 6
povidone
silver nanoparticle
acute toxicity
animal experiment
animal tissue
antisepsis
Article
bacterial load
biocompatibility
cell infiltration
controlled study
Escherichia coli
gene ontology
hemolysis
histology
in vitro study
inflammation
LD50
liver cell
male
mass spectrometry
methicillin resistant Staphylococcus aureus infection
morbidity
mortality
mortality rate
mouse
nonhuman
public health
spleen
M3 - Article
PY - 2023
ST - Serum protein coating enhances the antisepsis efficacy of silver
nanoparticles against multidrug-resistant Escherichia coli infections in mice
TI - Serum protein coating enhances the antisepsis efficacy of silver
nanoparticles against multidrug-resistant Escherichia coli infections in mice
85161218779&doi=10.3389%2ffmicb.2023.1153147&partnerID=40&md5=d0f7cd708c38bcacdf122
a14fcd4469a
VL - 14
ID - 5043
ER -
TY - JOUR
AB - The toxicity mechanism of nanoparticles on vertebrate cardiovascular system
is still unclear, especially on the low-level exposure. This study was to explore
the toxic effect and mechanisms of low-dose exposure of silica nanoparticles
(SiNPs) on cardiac function in zebrafish embryos via the intravenous
microinjection. The dosage of SiNPs was based on the no observed adverse effect
level (NOAEL) of malformation assessment in zebrafish embryos. The mainly cardiac
toxicity phenotypes induced by SiNPs were pericardial edema and bradycardia but had
no effect on atrioventricular block. Using o-Dianisidine for erythrocyte staining,
the cardiac output of zebrafish embryos was decreased in a dose-dependent manner.
Microarray analysis and bioinformatics analysis were performed to screen the
differential expression genes and possible pathway involved in cardiac function.
SiNPs induced whole-embryo oxidative stress and neutrophil-mediated cardiac
inflammation in Tg(mpo:GFP) zebrafish. Inflammatory cells were observed in atrium
of SiNPs-treated zebrafish heart by histopathological examination. In addition, the
expression of TNNT2 protein, a cardiac contraction marker in heart tissue had been
down-regulated compared to control group using immunohistochemistry. Confirmed by
qRT-PCR and western blot assays, results showed that SiNPs inhibited the calcium
signaling pathway and cardiac muscle contraction via the down-regulated of related
genes, such as ATPase-related genes (atp2a1l, atp1b2b, atp1a3b), calcium channel-
related genes (cacna1ab, cacna1da) and the regulatory gene tnnc1a for cardiac
troponin C. Moreover, the protein level of TNNT2 was decreased in a dose-dependent
manner. For the first time, our results demonstrated that SiNPs induced cardiac
dysfunction via the neutrophil-mediated cardiac inflammation and cardiac
contraction in zebrafish embryos.
AN - WOS:000372756300007
AU - Duan, J. C.
AU - Yu, Y.
AU - Li, Y.
AU - Li, Y. B.
AU - Liu, H. C.
AU - Jing, L.
AU - Yang, M.
AU - Wang, J.
AU - Li, C. Q.
AU - Sun, Z. W.
DA - MAY 27
DO - 10.3109/17435390.2015.1102981
IS - 5
PY - 2016
SN - 1743-5390
1743-5404
SP - 575-585
ST - Low-dose exposure of silica nanoparticles induces cardiac dysfunction via
neutrophil-mediated inflammation and cardiac contraction in zebrafish embryos
T2 - NANOTOXICOLOGY
TI - Low-dose exposure of silica nanoparticles induces cardiac dysfunction via
neutrophil-mediated inflammation and cardiac contraction in zebrafish embryos
VL - 10
ID - 6709
ER -
TY - JOUR
AB - Nanoparticles (NPs) are widely used in consumer and medicinal products. The
high prevalence of nanoparticles in the environment raises concerns regarding their
effects on human health, but there is limited knowledge about how NPs interact with
cells or tissues. Because the European Union has called for a substantial reduction
of animal experiments for scientific purposes (Directive 2010/63), increased
efforts are required to develop in vitro models to evaluate potentially hazardous
agents. Here, we describe a new cell-based biosensor for the evaluation of NPs
cytotoxicity. The new biosensor is based on transgenic human hepatoblastoma cells
(HepG2) that express a secreted form of alkaline phosphatase (SEAP) as a reporter
protein whose expression is induced upon activation of a stress response pathway
controlled by the transcription regulator nuclear factor-kappa B (NF-kappa B). The
NF-kappa B_HepG2 sensor cells were cultured in a Matrigel-based three dimensional
environment to simulate the in vivo situation. The new biosensor cells offer the
advantage of generating fast and reproducible readout at lower concentrations and
shorter incubation time than conventional viability assays, avoid possible
interaction between nanomaterials and assay compounds, therefore, minimize
generation of false positive or negative results and indicate mechanism of toxicity
through NF-kappa B signaling. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
AN - WOS:000387629700006
AU - Dubiak-Szepietowska, M.
AU - Karczmarczyk, A.
AU - Winckler, T.
AU - Feller, K. H.
DA - AUG 31
DO - 10.1016/j.tox.2016.09.012
PY - 2016
SN - 0300-483X
SP - 60-69
ST - A cell-based biosensor for nanomaterials cytotoxicity assessment in three
dimensional cell culture
T2 - TOXICOLOGY
TI - A cell-based biosensor for nanomaterials cytotoxicity assessment in three
dimensional cell culture
VL - 370
ID - 6234
ER -
TY - JOUR
AB - Nanoparticles found in air pollutants can alter neurotransmitter profiles,
increase neuroinflammation, and alter brain function. Therefore, the assay
described here will aid in elucidating the role of microglia in neuroinflammation
and neurodegenerative diseases. The use of microglia, resident immune cells of the
brain, as a surrogate biosensor provides novel insight into how inflammatory
responses mediate neuronal insults. Here, we utilize an immortalized murine
microglial cell line, designated BV2, and describe a method for nanoparticle
exposure using silver nanoparticles (AgNPs) as a standard. We describe how to
expose microglia to nanoparticles, how to remove nanoparticles from supernatant,
and how to use supernatant from activated microglia to determine toxicity, using
hypothalamic cell survival as a measure. Following AgNP exposure, BV2 microglial
activation was validated using a tumor necrosis factor alpha (TNF-α) enzyme linked
immunosorbent assay (ELISA). The supernatant was filtered to remove the AgNP and to
allow cytokines and other secreted factors to remain in the conditioned media.
Hypothalamic cells were then exposed to supernatant from AgNP activated microglia
and survival of neurons was determined using a resazurinbased fluorescent assay.
This technique is useful for utilizing microglia as a surrogate biomarker of
neuroinflammation and determining the effect of neuroinflammation on other cell
types. © 2016 Journal of Visualized Experiments.
AU - Duffy, C. M.
AU - Ahmed, S.
AU - Yuan, C.
AU - Mavanji, V.
AU - Nixon, J. P.
AU - Butterick, T.
C7 - e54662
DB - Scopus
DO - 10.3791/54662
IS - 116
KW - Cell death
Conditioned media
Hypothalamic cells
Issue 116
Microglia
Nanoparticle
Neuroinflammation
Neuroscience
Animals
Biosensing Techniques
Cell Line
Cell Survival
Cells, Cultured
Hypothalamus
Mice
Nanoparticles
Toxicity Tests
nanoparticle
animal
cell culture
cell line
cell survival
genetic procedures
hypothalamus
microglia
mouse
toxicity testing
M3 - Article
PY - 2016
ST - Microglia as a surrogate biosensor to determine nanoparticle neurotoxicity
T2 - Journal of Visualized Experiments
TI - Microglia as a surrogate biosensor to determine nanoparticle neurotoxicity
84992521894&doi=10.3791%2f54662&partnerID=40&md5=d4b495cc0f85b4c7863c9cba1776b6d5
VL - 2016
ID - 5527
ER -
TY - JOUR
AB - Fc-related antibody activities, such as antibody-dependent cellular
cytotoxicity (ADCC), or more broadly, antibody-mediated cellular viral inhibition
(ADCVI), play a role in curbing early SIV viral replication, are enriched in human
long-term infected nonprogressors, and could potentially contribute to protection
from infection. However, little is known about the mechanism by which such humoral
immune responses are naturally induced following infection. Here, we focused on the
early evolution of the functional antibody response, largely driven by the Fc
portion of the antibody, in the context of the evolving binding and neutralizing
antibody response, which is driven mainly by the antibody-binding fragment (Fab).
We show that ADCVI/ ADCC-inducing responses in humans are rapidly generated
following acute HIV-1 infection, peak at approximately 6 months postinfection, but
decay rapidly in the setting of persistent immune activation, as Fab-related
activities persistently increase. Moreover, the loss of Fc activity occurred in
synchrony with a loss of HIV-specific IgG3 responses. Our data strongly suggest
that Fc- and Fab-related antibody functions are modulated in a distinct manner
following acute HIV infection. Vaccination strategies intended to optimally induce
both sets of antiviral antibody activities may, therefore, require a fine tuning of
the inflammatory response.
AN - WOS:000343827600011
AU - Dugast, A. S.
AU - Stamatatos, L.
AU - Tonelli, A.
AU - Suscovich, T. J.
AU - Licht, A. F.
AU - Mikell, I.
AU - Ackerman, M. E.
AU - Streeck, H.
AU - Klasse, P. J.
AU - Moore, J. P.
AU - Alter, G.
DA - OCT
DO - 10.1002/eji.201344305
IS - 10
PY - 2014
SN - 0014-2980
1521-4141
SP - 2925-2937
ST - Independent evolution of Fc- and Fab-mediated HIV-1-specific antiviral
antibody activity following acute infection
T2 - EUROPEAN JOURNAL OF IMMUNOLOGY
TI - Independent evolution of Fc- and Fab-mediated HIV-1-specific antiviral
antibody activity following acute infection
VL - 44
ID - 6830
ER -
TY - JOUR
AB - For the last years scientific community has witnessed a rapid development of
novel types of biomaterials, which properties made them applicable in numerous
fields of medicine. Although nanosilver, well-known for its antimicrobial, anti-
angiogenic, anti-inflammatory and anticancer activities, as well as hyaluronic
acid, a natural polysaccharide playing a vital role in the modulation of tissue
repair, signal transduction, angiogenesis, cell motility and cancer metastasis, are
both thoroughly described in the literature, their complexes are still a novel
topic. In this review we introduce the most recent research about the synthesis,
properties, and potential applications of HA-nanosilver composites. We also make an
attempt to explain the variety of mechanisms involved in their action. Finally, we
present biocompatible and biodegradable complexes with bactericidal activity and
low cytotoxicity, which properties suggest their suitability for the prophylaxis
and therapy of chronic wounds, as well as analgetic therapies, anticancer
strategies and the detection of chemical substances and malignant cells. Cited
studies reveal that the usage of hyaluronic acid-silver nanocomposites appears to
be efficient and safe in clinical practice. © 2021 by the authors. Licensee MDPI,
Basel, Switzerland.
AU - Dulińska-Litewka, J.
AU - Dykas, K.
AU - Felkle, D.
AU - Karnas, K.
AU - Khachatryan, G.
AU - Karewicz, A.
C7 - 234
DB - Scopus
DO - 10.3390/ma15010234
IS - 1
KW - Biopolymers
Hyaluronic acid
Nanocomposites
Polysaccharides
Biocompatibility
Chemical detection
Diseases
Organic acids
Signal transduction
Angiogenesis
Anticancer activities
Nano silver
Natural polysaccharide
Property
Scientific community
Silver nanocomposites
Tissue repair
M3 - Article
PY - 2022
ST - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
T2 - Materials
TI - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
85122931311&doi=10.3390%2fma15010234&partnerID=40&md5=c67a9f3a5af61ee87db7e54f744a2
b8b
VL - 15
ID - 5137
ER -
TY - JOUR
AB - For the last years scientific community has witnessed a rapid development of
novel types of biomaterials, which properties made them applicable in numerous
fields of medicine. Although nanosilver, well-known for its antimicrobial, anti-
angiogenic, anti-inflammatory and anticancer activities, as well as hyaluronic
acid, a natural polysaccharide playing a vital role in the modulation of tissue
repair, signal transduction, angiogenesis, cell motility and cancer metastasis, are
both thoroughly described in the literature, their complexes are still a novel
topic. In this review we introduce the most recent research about the synthesis,
properties, and potential applications of HA-nanosilver composites. We also make an
attempt to explain the variety of mechanisms involved in their action. Finally, we
present biocompatible and biodegradable complexes with bactericidal activity and
low cytotoxicity, which properties suggest their suitability for the prophylaxis
and therapy of chronic wounds, as well as analgetic therapies, anticancer
strategies and the detection of chemical substances and malignant cells. Cited
studies reveal that the usage of hyaluronic acid-silver nanocomposites appears to
be efficient and safe in clinical practice.
AN - WOS:000758547300001
AU - Dulinska-Litewka, J.
AU - Dykas, K.
AU - Felkle, D.
AU - Karnas, K.
AU - Khachatryan, G.
AU - Karewicz, A.
C7 - 234
DA - JAN
DO - 10.3390/ma15010234
IS - 1
PY - 2022
SN - 1996-1944
ST - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
T2 - MATERIALS
TI - Hyaluronic Acid-Silver Nanocomposites and Their Biomedical Applications: A
Review
VL - 15
ID - 6135
ER -
TY - JOUR
AB - BackgroundSepsis biomarker panels that provide diagnostic and prognostic
discrimination in sepsis patients would be transformative to patient care. We
assessed the mortality prediction and diagnostic discriminatory accuracy of two
biomarkers reflective of cell death (apoptosis), circulating cell-free DNA (cfDNA),
and nucleosomes.MethodsThe cfDNA and nucleosome levels were assayed in plasma
samples acquired in patients admitted from four emergency departments with
suspected sepsis. Subjects with non-infectious systemic inflammatory response
syndrome (SIRS) served as controls. Samples were acquired at enrollment (T0) and
24h later (T24). We assessed diagnostic (differentiating SIRS from sepsis) and
prognostic (28-day mortality) predictive power. Models incorporating procalcitonin
(diagnostic prediction) and APACHE II scores (mortality prediction) were
generated.ResultsTwo hundred three subjects were included (107 provided
procalcitonin measurements). Four subjects exhibited uncomplicated sepsis, 127
severe sepsis, 35 septic shock, and 24 had non-infectious SIRS. There were 190-
survivors and 13 non-survivors. Mortality prediction models using cfDNA,
nucleosomes, or APACHEII yielded AUC values of 0.61, 0.75, and 0.81, respectively.
A model combining nucleosomes with the APACHE II score improved the AUC to 0.84.
Diagnostic models distinguishing sepsis from SIRS using procalcitonin, cfDNA(T0),
or nucleosomes(T0) yielded AUC values of 0.64, 0.65, and 0.63, respectively. The
three parameter model yielded an AUC of 0.74.ConclusionsTo our knowledge, this is
the first head-to-head comparison of cfDNA and nucleosomes in diagnosing sepsis and
predicting sepsis-related mortality. Both cfDNA and nucleosome concentrations
demonstrated a modest ability to distinguish sepsis survivors and non-survivors and
provided additive diagnostic predictive accuracy in differentiating sepsis from
non-infectious SIRS when integrated into a diagnostic prediction model including
PCT and APACHE II. A sepsis biomarker strategy incorporating measures of the
apoptotic pathway may serve as an important component of a sepsis diagnostic and
mortality prediction tool.
AN - WOS:000450522500001
AU - Duplessis, C.
AU - Gregory, M.
AU - Frey, K.
AU - Bell, M.
AU - Truong, L.
AU - Schully, K.
AU - Lawler, J.
AU - Langley, R. J.
AU - Kingsmore, S. F.
AU - Woods, C. W.
AU - Rivers, E. P.
AU - Jaehne, A. K.
AU - Quackenbush, E. B.
AU - Fowler, V. G.
AU - Tsalik, E. L.
AU - Clark, D.
C7 - 72
DA - NOV 13
DO - 10.1186/s40560-018-0341-5
PY - 2018
SN - 2052-0492
ST - Evaluating the discriminating capacity of cell death (apoptotic) biomarkers
in sepsis
T2 - JOURNAL OF INTENSIVE CARE
TI - Evaluating the discriminating capacity of cell death (apoptotic) biomarkers
in sepsis
VL - 6
ID - 6640
ER -
TY - JOUR
AB - Rhamnolipids extracted from Pseudomonas aeruginosa strain JS-11 were utilized
for synthesis of stable silver nanoparticles (Rh-AgNPs). The Rh-AgNPs (23nm) were
characterized by Fourier transform infra-red (FTIR) spectroscopy, atomic force
microscopy (AFM) and transmission electron microscopy (TEM). The cytotoxicity
assays suggested significant decrease in viability of Rh-AgNPs treated human breast
adenocarcinoma (MCF-7) cells, compared with normal human peripheral blood
mononuclear (PBMN) cells. Flow cytometry data revealed 1.25-fold (p<0.05) increase
in the fluorescence of 2',7'-dichlorofluorescein (DCF) at 0.25μg/mL. However, at
Rh-AgNPs concentrations of 0.5 and 1.0μg/mL, much lesser fluorescence was noticed,
which is attributed to cell death. Results with the fluorescent probe Rh123
demonstrated change in inner mitochondrial membrane and dissipation of membrane
potential. The cell cycle analysis suggested 19.9% (p<0.05) increase in sub-G1 peak
with concomitant reduction in G1 phase at 1μg/mL of Rh-AgNPs, compared to 2.7% in
untreated control. The real-time RT2 Profiler™ PCR array data elucidated the
overexpression of seven oxidative stress and DNA damage pathways genes viz. BAX,
BCl2, Cyclin D1, DNAJA1, E2F transcription factor 1, GPX1 and HSPA4, associated
with apoptosis signaling, proliferation and carcinogenesis, pro inflammatory and
heat shock responses in Rh-AgNPs treated cells. Thus, the increased ROS production,
mitochondrial damage and appearance of sub-G1 (apoptotic) population suggested the
anti-proliferative activity, and role of oxidative stress pathway genes in Rh-AgNPs
induced death of MCF-7 cancer cells. © 2015 Elsevier B.V.
AU - Dwivedi, S.
AU - Saquib, Q.
AU - Al-Khedhairy, A. A.
AU - Ahmad, J.
AU - Siddiqui, M. A.
AU - Musarrat, J.
DB - Scopus
DO - 10.1016/j.colsurfb.2015.05.034
KW - Cytotoxicity
Gene expression
Rhamnolipid
ROS
XRD
Breast Neoplasms
Cell Death
Female
Gene Expression
Humans
Lipids
MCF-7 Cells
Metal Nanoparticles
Oxidative Stress
Silver
X-Ray Diffraction
Atomic force microscopy
Cell death
Cell membranes
Cells
Cytology
Fluorescence
Genes
Mitochondria
Nanoparticles
Oxidative stress
Rhodium
Surface active agents
Transcription
placebo
rhamnolipid
silver nanoparticle
lipid
metal nanoparticle
silver
Fourier transform infra red (FTIR) spectroscopy
Human peripheral blood
Inner mitochondrial membranes
Rhamnolipids
apoptosis
Article
BAX gene
BCL2 gene
cancer cell culture
carcinogenesis
cell proliferation
cell stress
cell structure
cell viability
cellular stress response
controlled study
CyclinD1 gene
cytotoxicity assay
cytotoxicity test
DNA damage
DNAJA1 gene
drug determination
drug isolation
drug stability
drug structure
drug synthesis
E2F transcription factor 1 gene
flow cytometry
fluorescence analysis
gene
gene function
gene identification
gene location
gene overexpression
GPX1 gene
heat shock response
HSPA4 gene
human
human cell
inflammation
lipid analysis
mitochondrial toxicity
molecular probe
oxidative stress
priority journal
signal transduction
toxicity testing
zeta potential
breast tumor
cell death
chemistry
drug effects
female
gene expression
genetics
MCF 7 cell line
metabolism
pathology
X ray diffraction
Cell signaling
M3 - Article
PY - 2015
SP - 290-298
ST - Rhamnolipids functionalized AgNPs-induced oxidative stress and modulation of
toxicity pathway genes in cultured MCF-7 cells
TI - Rhamnolipids functionalized AgNPs-induced oxidative stress and modulation of
toxicity pathway genes in cultured MCF-7 cells
84936114732&doi=10.1016%2fj.colsurfb.2015.05.034&partnerID=40&md5=aebbdc218402f05cc
6cbe439f5292e47
VL - 132
ID - 5634
ER -
TY - JOUR
AB - Following spinal cord injury (SCI), the population of mature oligodendrocytes
undergoes substantial cell death; promoting their preservation and replacement is a
viable strategy for preserving axonal integrity and white matter repair in the
injured spinal cord. Dramatic upregulation of matrix chondroitin sulfate
proteoglycans (CSPGs) is shown to pose an obstacle to endogenous repair processes,
and targeting CSPGs improves functional recovery after SCI. However, the cellular
and molecular mechanisms underlying the inhibitory effects of CSPGs remain largely
undefined. Modulation of CSPGs specific signaling receptors, leukocyte common
antigen-related (LAR), and protein tyrosine phosphatase-sigma (PTP sigma) allows us
to uncover the role and mechanisms of CSPGs in regulating oligodendrocytes in SCI.
Here, utilizing specific functionally blocking peptides in a clinically relevant
model of contusive/compressive SCI in the rat, we demonstrate that inhibition of
PTP sigma and LAR receptors promotes oligodendrogenesis by endogenous precursor
cells, attenuates caspase 3-mediated cell death in mature oligodendrocytes, and
preserves myelin. In parallel in vitro systems, we have unraveled that CSPGs
directly induce apoptosis in populations of neural precursor cells and
oligodendrocyte progenitor cells and limit their ability for oligodendrocyte
differentiation, maturation, and myelination. These negative effects of CSPGs are
mediated through the activation of both LAR and PTP sigma receptors and the
downstream Rho/ROCK pathway. Thus, we have identified a novel inhibitory role for
PTP sigma and LAR in regulating oligodendrocyte differentiation and apoptosis in
the injured adult spinal cord and a new feasible therapeutic strategy for
optimizing endogenous cell replacement following SCI.
AN - WOS:000454601200010
AU - Dyck, S.
AU - Kataria, H.
AU - Akbari-Kelachayeh, K.
AU - Silver, J.
AU - Karinni-Abdolrezaee, S.
DA - JAN
DO - 10.1002/glia.23533
IS - 1
PY - 2019
SN - 0894-1491
1098-1136
SP - 125-145
ST - LAR and PTP sigma receptors are negative regulators of oligodendrogenesis and
oligodendrocyte integrity in spinal cord injury
T2 - GLIA
TI - LAR and PTP sigma receptors are negative regulators of oligodendrogenesis and
oligodendrocyte integrity in spinal cord injury
VL - 67
ID - 6617
ER -
TY - JOUR
AB - Due to their potent antibacterial properties, silver nanoparticles (AgNPs)
are widely used in industry and medicine. However, they can cross the brain-blood
barrier, posing a risk to the brain and its functions. In our previous study, we
demonstrated that oral administration of bovine serum albumin (BSA)-coated AgNPs
caused an impairment in spatial memory in a dose-independent manner. In this study,
we evaluated the effects of AgNPs coating material on cognition, spatial memory
functioning, and neurotransmitter levels in rat hippocampus. AgNPs coated with BSA
(AgNPs(BSA)), polyethylene glycol (AgNPs(PEG)), or citrate (AgNPs(Cit)) or silver
ions (Ag+) were orally administered at a dose of 0.5 mg/kg b.w. to male Wistar rats
for a period of 28 days, while the control (Ctrl) rats received 0.2 mL of water.
The acquisition and maintenance of spatial memory related to place avoidance were
assessed using the active allothetic place avoidance task, in which rats from
AgNPs(BSA), AgNPs(PEG), and Ag+ groups performed worse than the Ctrl rats. In the
retrieval test assessing long-term memory, only rats from AgNPs(Cit) and Ctrl
groups showed memory maintenance. The analysis of neurotransmitter levels indicated
that the ratio between serotonin and dopamine concentration was disturbed in the
AgNPs(BSA) rats. Furthermore, treatment with AgNPs or Ag+ resulted in the induction
of peripheral inflammation, which was reflected by the alterations in the levels of
serum inflammatory mediators. In conclusion, depending on the coating material used
for their stabilization, AgNPs induced changes in memory functioning and
concentration of neurotransmitters.
AN - WOS:000735642700001
AU - Dziendzikowska, K.
AU - Wesierska, M.
AU - Gromadzka-Ostrowska, J.
AU - Wilczak, J.
AU - Oczkowski, M.
AU - Meczynska-Wielgosz, S.
AU - Kruszewski, M.
C7 - 12706
DA - DEC
DO - 10.3390/ijms222312706
IS - 23
PY - 2021
SN - 1422-0067
ST - Silver Nanoparticles Impair Cognitive Functions and Modify the Hippocampal
Level of Neurotransmitters in a Coating-Dependent Manner
TI - Silver Nanoparticles Impair Cognitive Functions and Modify the Hippocampal
Level of Neurotransmitters in a Coating-Dependent Manner
VL - 22
ID - 6129
ER -
TY - JOUR
AB - Due to undesirable hazardous interactions with biological systems, we
evaluated the effect of silver nanoparticles (AgNPs) intake on oxidative stress and
inflammation. Rats received for 81. days a standard diet (Controls) or a standard
diet plus 500. mg/d/kg BW AgNPs. We assayed plasma lipids, and oxidative stress was
assessed by measuring liver and heart superoxide anion production (O2°-) and liver
malondialdehyde levels (MDA). Antioxidant status was appraised using plasma
paraoxonase activity (PON), plasma antioxidant capacity (PAC) and liver superoxide
dismutase activity (SOD). Liver inflammatory cytokines TNFα and IL-6 levels and
plasma alanine aminotransferase (ALT) were assayed. Compared with Controls, AgNPs
raised cholesterolemia (9.5%), LDL-cholesterol (30%), and lowered triglycerides
(41%). They also increased liver (30%) and cardiac (41%) O2°- production, reduced
PON activity (15%) and raised liver TNFα (9%) and IL-6 (~12%). Plasma ALT activity
rose (12%) after treatment with AgNPs. However, PAC and liver MDA and SOD activity
were unchanged. These features indicate that exposure to 500. mg/d/kg BW of AgNPs
results in liver damage by a dysregulation of lipid metabolism, highlighting liver
and heart as the most sensitive organs to the deleterious effects. Our findings
also demonstrate for the first time the oxidative and inflammatory effects of
dietary AgNPs. © 2013 Elsevier Ltd.
AU - Ebabe Elle, R.
AU - Gaillet, S.
AU - Vidé, J.
AU - Romain, C.
AU - Lauret, C.
AU - Rugani, N.
AU - Cristol, J. P.
AU - Rouanet, J. M.
DB - Scopus
DO - 10.1016/j.fct.2013.07.071
KW - Collargol
Inflammation
Oxidative stress
Rats
Toxicity
Administration, Oral
Alanine Transaminase
Animals
Antioxidants
Cholesterol
Heart
Hypercholesterolemia
Interleukin-6
Lipid Metabolism
Liver
Liver Diseases
Male
Malondialdehyde
Metal Nanoparticles
Oxidative Stress
Silver
Superoxides
Triglycerides
aryldialkylphosphatase
interleukin 6
low density lipoprotein cholesterol
malonaldehyde
silver nanoparticle
triacylglycerol
animal experiment
animal model
animal tissue
article
body weight
cardiotoxicity
controlled study
enzyme activity
enzyme blood level
food intake
hypercholesterolemia
inflammation
lipid metabolism
liver level
liver toxicity
male
nonhuman
oxidative stress
rat
M3 - Article
PY - 2013
SP - 297-301
ST - Dietary exposure to silver nanoparticles in Sprague-Dawley rats: Effects on
oxidative stress and inflammation
T2 - Food and Chemical Toxicology
TI - Dietary exposure to silver nanoparticles in Sprague-Dawley rats: Effects on
oxidative stress and inflammation
84882949984&doi=10.1016%2fj.fct.2013.07.071&partnerID=40&md5=bb69426426544421ee0aff
ca56758b47
VL - 60
ID - 5727
ER -
TY - JOUR
AB - Leishmaniasis, classified as a neglected tropical disease (NTD), is a
parasitic infection caused by protozoa and transmitted through vectors. It is a
widespread condition found in over 100 countries, known for its destructive nature.
Consequently, there is an increasing need to establish novel approaches for
combating leishmaniasis and developing effective strategies to combat the disease.
In this study, we report an eco-friendly, cost-effective, and biocompatible process
for the preparation of silver nanoparticles (AgNPs) using aqueous extract of Crocus
sativus petals and their antibacterial and anti-leishmanial activities.
Characterization of biosynthesized AgNPs was conducted by UV-vis spectroscopy, TEM,
FTIR, DLS, and FESEM spectroscopy. TEM and FESEM images revealed the existence of
spherical/oval morphology with a size range of 30–70 nm. Additionally, functional
groups associated with the extract were observed, indicating their involvement in
the stabilization and coating of the biologically fabricated AgNPs. Furthermore,
the AgNPs were confirmed through the observation of a surface plasmon response
(SPR) with a peak wavelength of approximately 423 nm. This was accompanied by
noticeable color transformation from transparent to brown, providing further
evidence of the successful formation of AgNPs. Biosynthesized AgNPs from saffron
wastages showed promising anti-leishmanial and antibacterial activities. The AgNPs
displayed a zone of inhibition of 23 mm against Staphylococcus aureus and 10 mm
against Pseudomonas aeruginosa, indicating their potential antibacterial
performance against these microorganisms. The application of an ointment containing
quercetin/saffron-capped silver nanoparticles on mice infected with Leishmania
major resulted in promising outcomes. The treated group exhibited a reduction in
inflammatory responses and an increase in fibroblast activity compared to the
untreated group. These results indicate the potential of this compound to alleviate
inflammation and promote the growth of fibroblasts, which are beneficial for wound
healing and tissue repair. In conclusion, the utilization of biogenically
synthesized silver nanoparticles derived from saffron holds promise as an
alternative therapeutic approach for treating cutaneous leishmaniasis. © 2023, The
Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of
Springer Nature.
AU - Ebrahimzadeh, A.
AU - Karamian, M.
AU - Alemzadeh, E.
AU - Solgi, R.
AU - Mortazavi-Derazkola, S.
DB - Scopus
DO - 10.1007/s13399-023-04630-x
KW - Anti-leishmanial
Antibacterial
Green synthesis
Saffron wastages
Bacteria
Biocompatibility
Cost effectiveness
Fibroblasts
Mammals
Metal nanoparticles
Morphology
Surface plasmons
Antibacterials
Classifieds
Crocus sativus
Cutaneous leishmaniasis
Neglected tropical disease
Parasitic infections
Saffron wastage
M3 - Article
PY - 2023
ST - Investigation of therapeutic potential in murine cutaneous leishmaniasis and
antibacterial using biosynthesized silver nanoparticles using extract of Crocus
sativus petals
T2 - Biomass Conversion and Biorefinery
TI - Investigation of therapeutic potential in murine cutaneous leishmaniasis and
antibacterial using biosynthesized silver nanoparticles using extract of Crocus
sativus petals
85165259721&doi=10.1007%2fs13399-023-04630-
x&partnerID=40&md5=86b90627dcbe89c25c1786cdc52dacbd
ID - 5032
ER -
TY - JOUR
AB - Four root canal sealers (AH-26, Roth 811, CRCS, and Sealapex) were tested for
tissue biocompatibility in rat connective tissue. Each sealer was placed in Teflon
tubes and implanted subcutaneously in Wistar-Furth rats. The implants were removed
after 7, 14, and 21 days, fixed, and histologically prepared for microscopical
evaluation. Brain, liver, kidneys, and uterus were removed from the animals killed
at the first experimental period (7 days) and analyzed for zinc and calcium
concentration by flame atomic absorption spectrophotometry. In total, 100 specimens
were examined. At the seventh day, the most irritant material was seen to be AH-26,
but this inflammatory reaction decreased with time. Roth 811 and Sealapex caused
moderate-to-severe inflammatory reaction, whereas CRCS caused mild to moderate.
CRCS and Roth 811 induced redistrubution of zinc, whereas AH-26 induced changes in
calcium content in some organs. © 1995 The American Association of Endodontists.
AU - Economides, N.
AU - Kotsaki-Kovatsi, V. P.
AU - Poulopoulos, A.
AU - Kolokuris, I.
AU - Rozos, G.
AU - Shore, R.
DB - Scopus
DO - 10.1016/S0099-2399(06)80436-X
IS - 3
KW - Animal
Bismuth
Brain Chemistry
Calcium
Calcium Hydroxide
Comparative Study
Connective Tissue
Drug Combinations
Female
Foreign-Body Reaction
Kidney
Liver
Methenamine
Rats
Rats, Wistar
Silver
Tissue Distribution
Titanium
Uterus
Zinc
Zinc Oxide
biomaterial
bismuth
Calcibiotic Root Canal Sealer
calcium
calcium hydroxide
methenamine
Roth's 811 sealer
sealapex
silver
titanium
zinc
zinc oxide
zinc oxide eugenol
animal
article
brain level
chemistry
comparative study
connective tissue
drug combination
drug effect
female
foreign body reaction
kidney
liver
metabolism
rat
rat strain
tissue distribution
uterus
M3 - Article
PY - 1995
SP - 122-127
ST - Experimental study of the biocompatibility of four root canal sealers and
their influence on the zinc and calcium content of several tissues
TI - Experimental study of the biocompatibility of four root canal sealers and
their influence on the zinc and calcium content of several tissues
0029268077&doi=10.1016%2fS0099-2399%2806%2980436-
X&partnerID=40&md5=b2057f4559d36783a0d2ad95ac72d82a
VL - 21
ID - 5803
ER -
TY - JOUR
AB - Drug-loaded nanoparticles are currently gaining attention due to their
improved drug delivery properties. Apocynin, a natural polyphenolic compound, is a
component of many plants. It has many medicinal and pharmacological properties.
Pyrogallol is an anti-psoriatic agent. However, its clinical usage is limited due
to its cumulative and dose-dependent hepatotoxicity. The objective of this study
was to synthesize silver nanoparticles coated with Apocynin (Apo-AgNPs), and
investigate the antioxidant and liver protective effects of Apo-AgNPs on
pyrogallol-induced toxicity in rats. The nanoparticles were characterized and it
was determined that the synthesis technique results in homogeneously dispersed
core–shell Ag structures with spherical forms and an average diameter of 13 nm (6.3
nm). Our results showed that Apo-AgNPs exhibited potent antioxidant and excellent
membrane stability activities in vitro. In rats, Apo-AgNPs (10 and 30 mg/kg)
significantly prevented pyrogallol-induced elevations of alkaline phosphatase,
gamma-glutamyl transferase, creatinine, urea, aspartate aminotransferase, alkaline
aminotransferase, total bilirubin, and decreased blood levels of uric acid.
Moreover, Apo-AgNPs restored the decreased activities of the liver antioxidant
enzymes, including superoxide dismutase and glutathione peroxidase, glutathione
transferase, as well as non-enzyme antioxidant glutathione, as well as
significantly decreased catalase activities which were induced by pyrogallol
treatment. Histological studies indicated that pyrogallol -induced liver damage was
alleviated following Apo-AgNPs treatment in rats. Apo-AgNPs significantly
suppressed the up-regulation of Cyclooxygenase-2 (COX-2), Interleukin 6 (IL-6) and
Nuclear factor-κB (NF-κB) protein expression. These results indicated that Apo-
AgNPs protected the rats from damage via preserving the antioxidant defense
systems, lowering pro-inflammatory cytokines, and expression of COX-2 and NF-κB in
rats. © 2022 Elsevier B.V.
AU - Ekozin, A.
AU - Otuechere, C. A.
AU - Adewuyi, A.
C7 - 110069
DB - Scopus
DO - 10.1016/j.cbi.2022.110069
KW - Ampocynin
Hepatic toxicity marker
Nanoparticle
Oxidative stress
Pyrogallol
Acetophenones
Animals
Antioxidants
Chemical and Drug Induced Liver Injury
Cyclooxygenase 2
Lipid Peroxidation
Liver
Metal Nanoparticles
NF-kappa B
Oxidative Stress
Rats
Silver
antioxidant
apocynin
bilirubin
creatinine
cyclooxygenase 2
gamma glutamyltransferase
glutathione
hydrogen peroxide
interleukin 6
malonaldehyde
nitric oxide
pyrogallol
silver nanoparticle
thiobarbituric acid
urea
uric acid
acetophenone derivative
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
blood biochemistry
cervical spine dislocation
controlled study
histology
histopathology
in vitro study
lipid peroxidation
liver protection
liver toxicity
male
nonhuman
oxidative stress
physical chemistry
protein expression
Raman spectrometry
rat
thermogravimetry
thermostability
animal
chemistry
liver
metabolism
toxic hepatitis
M3 - Article
PY - 2022
ST - Apocynin loaded silver nanoparticles displays potent in vitro biological
activities and mitigates pyrogallol-induced hepatotoxicity
T2 - Chemico-Biological Interactions
TI - Apocynin loaded silver nanoparticles displays potent in vitro biological
activities and mitigates pyrogallol-induced hepatotoxicity
85135704587&doi=10.1016%2fj.cbi.2022.110069&partnerID=40&md5=83e5e7bacbdbcf607f1726
4512cafb80
VL - 365
ID - 5063
ER -
TY - JOUR
AB - There is serious concern about the potential harmful effects of certain
nanomaterials (NMs), on account of their ability to penetrate cell membranes and
the increased reactivity that results from their increased surface area compared
with bulk chemicals. To assess the safety of NMs, reliable tests are needed. We
have investigated the possible genotoxicity of four representative NMs, derived
from titanium dioxide, zinc oxide, cerium oxide and silver, in two human cell
lines, A549 alveolar epithelial cells and lymphoblastoid TK6 cells. A high-
throughput version of the comet assay was used to measure DNA strand beaks (SBs) as
well as oxidised purines (converted to breaks with the enzyme formamidopyrimidine
DNA glycosylase). In parallel, cytotoxicity was measured with the alamarBlue (R)
assay, and the ability of NM-treated cells to survive was assessed by their colony-
forming efficiency. TiO2 and CeO2 NMs were only slightly cytotoxic by the
alamarBlue r test, and had no long-term effect on colony-forming efficiency.
However, both induced DNA damage at non-cytotoxic concentrations; the damage
decreased from 3 to 24-h exposure, except in the case of CeO2 -treated A549 cells.
ZnO and Ag NMs affected cell survival, and induced high levels of DNA damage at
cytotoxic concentrations. At lower concentrations, there was significant damage,
which tended to persist over 24 h. The implication is that all four reference metal
NMs tested-whether cytotoxic or not-are genotoxic. A full assessment of NM toxicity
should include tests on different cell types, different times of incubation and a
wide range of (especially non-cytotoxic) concentrations; a test for cell viability
should be performed in parallel. Inclusion of Fpg in the comet assay allows
detection of indirect genotoxic effects via oxidative stress.
AN - WOS:000397070600011
AU - El Yamani, N.
AU - Collins, A. R.
AU - Runden-Pran, E.
AU - Fjellsbo, L. M.
AU - Shaposhnikov, S.
AU - Zienolddiny, S.
AU - Dusinska, M.
DA - JAN
DO - 10.1093/mutage/gew060
IS - 1
PY - 2017
SN - 0267-8357
1464-3804
SP - 117-126
ST - In vitro genotoxicity testing of four reference metal nanomaterials, titanium
dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment
T2 - MUTAGENESIS
TI - In vitro genotoxicity testing of four reference metal nanomaterials, titanium
dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment
VL - 32
ID - 6442
ER -
TY - JOUR
AB - This review summarizes the four processes of wound healing in the human body
(hemostasis, inflammatory, proliferation, and remodeling) and the most current
research on the most important factors affecting cutaneous wound healing and the
underlying cellular and/or molecular pathways. Local factors, including
temperature, oxygenation, and infection, and systemic factors, such as age,
diabetes, sex hormones, genetic components, autoimmune diseases, psychological
stress, smoking and obesity are also addressed. A better understanding of the role
of these factors in wound repair could result in the development of therapeutics
that promote wound healing and resolve affected wounds. Additionally, natural
products obtained from plants and animals are critical targets for the discovery of
novel biologically significant pharmacophores, such as medicines and agrochemicals.
This review outlines the most recent advances in naturally derived targeted
treatment for wound healing. These are plant-derived natural products, insect-
derived natural products, marine-derived natural products, nanomaterial-based
wound-healing therapeutics (metal- and non-metal-based nanoparticles), and natural
product-based nanomedicine to improve the future direction of wound healing.
Natural products extracted from plants and animals have advanced significantly,
particularly in the treatment of wound healing. As a result, the isolation and
extraction of bioactive compounds from a variety of sources can continue to advance
our understanding of wound healing. Undescribed bioactive compounds or unexplored
formulations that could have a role in today's medicinal arsenal may be contained
in the abundance of natural products and natural product derivatives. © 2021
Elsevier Ltd
AU - El-Ashram, S.
AU - El-Samad, L. M.
AU - Basha, A. A.
AU - El Wakil, A.
C7 - 105749
DB - Scopus
DO - 10.1016/j.phrs.2021.105749
KW - Insect-derived natural products
Marine-derived natural products
Nanomaterial-based wound-healing therapeutics
Natural product-based nanomedicine
Plant-derived products
Wound healing
Animals
Aquatic Organisms
Biological Products
Humans
Insecta
Nanomedicine
Phytotherapy
Plant Preparations
Skin
Wound Healing
Wounds and Injuries
acaricide
agricultural chemical
apamin
bee venom
catalase
chitosan
chrysin
curcumin
galangin
gelatinase A
glibenclamide
glutathione
gold nanoparticle
graphene
herbaceous agent
hyaluronic acid
hydrogel
hydroxyproline
kaempferol
lipoxygenase
luteolin
metal nanoparticle
methylglyoxal
microRNA
nanocomposite
nanofiber
nanoparticle
natural product
netilmicin
nonmetal
oleic acid
pectin
plasmin
polyvinyl alcohol
quercetin
salicylic acid
saponin
sericin
sex hormone
silver nanoparticle
sunscreen
vasculotropin
biological product
plant medicinal product
Aloe vera
anemia
angiogenesis
antifungal activity
antiphospholipid syndrome
antiviral activity
aquatic environment
autoimmune disease
biocompatibility
biodegradability
cell migration
cell proliferation
cytokine production
cytotoxicity
debridement
depression
diabetes mellitus
diabetic foot
diabetic neuropathy
Down syndrome
drug formulation
drug release
dyspepsia
enzyme activity
episiotomy
epithelization
Escherichia coli
extraction
female
gangrene
gingivitis
glucose blood level
glycosylation
granulation tissue
hemostasis
hermaphrodite
honeybee
human
hyperglycemia
hyperoxia
hypertension
hyperthermia
hypoxia
inflammation
insect
Klinefelter syndrome
male
mental stress
molecularly targeted therapy
mouse
myofibroblast
nanomedicine
nonhuman
obesity
osteolysis
osteomyelitis
oxidative stress
oxygenation
phagocytosis
pharmacophore
quorum sensing
rat
regenerative medicine
Review
sepsis
sickle cell anemia
signal transduction
skin injury
skin irritation
skin water loss
smoking
systemic lupus erythematosus
tissue engineering
tissue regeneration
tonsillectomy
vagina flora
virus replication
wound dehiscence
wound healing
wound infection
animal
aquatic species
chemistry
drug effect
injury
metabolism
pathology
phytotherapy
skin
M3 - Review
PY - 2021
ST - Naturally-derived targeted therapy for wound healing: Beyond classical
strategies
T2 - Pharmacological Research
TI - Naturally-derived targeted therapy for wound healing: Beyond classical
strategies
85109002351&doi=10.1016%2fj.phrs.2021.105749&partnerID=40&md5=2a59937bec7cec08150f5
3c1d3942a1d
VL - 170
ID - 5225
ER -
TY - JOUR
AB - Bacterial infections are the key cause of death in patients suffering from
burns and diabetic wounds while the use of traditional antibiotics has been growing
steadily. Thus, in the present study, we are trying to introduce a paradigm shift
strategy to improve chronic wound healing of bacterial infection. To that end, we
have biologically synthesized silver nanoparticles (AgNPs) usingArthrospira
sppolysaccharides, and evaluated their antibacterial efficacy with their safety
pattern. Scanning electron micrographs showed spherical AgNPs coated with algal
polysaccharides with an approximate size of 9.7 nm. Treatment ofPseudomonas
aeruginosawith the AgNPs (0.5-1 mu g/mL) resulted in a significant disruption inP.
aeruginosaouter membrane, reduction in biofilm formation, and a significant
decrease of production of alginate and pyocyanin along with a concentration-
dependent reduction in beta-lactamase activity. In addition, at thein vivolevel,
AgNPs displayed substantial activity to controlP. aeruginosainfections in rat skin
wounds with significant reduction in in COX-2 enzyme in both rat skin homogenate
and serum samples. Furthermore, AgNPs facilitated wound curative in theP.
aeruginosainfected model by reducing the hemorrhagic areas number and the
infiltrated inflammatory cells. Taken all together, these biogenic nanoparticles
showed unique properties in controlling bacterial wound infections and improving
the healing process of damaged tissues via its direct and indirect effects.
AN - WOS:000560523500001
AU - El-Deeb, N. M.
AU - Abo-Eleneen, M. A.
AU - Al-Madboly, L. A.
AU - Sharaf, M. M.
AU - Othman, S. S.
AU - Ibrahim, O. M.
AU - Mubarak, M. S.
C7 - 643
DA - JUL 21
DO - 10.3389/fbioe.2020.00643
PY - 2020
SN - 2296-4185
ST - Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles:
Immunomodulatory and Antibacterial Potentialities Against ResistantPseudomonas
aeruginosa
T2 - FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
TI - Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles:
Immunomodulatory and Antibacterial Potentialities Against ResistantPseudomonas
aeruginosa
VL - 8
ID - 6024
ER -
TY - JOUR
AB - Plants belonging to the Launaea genus have been extensively utilized
ethnopharmacologically to treat a variety of diseases, including kidney disorders.
Chromium is a common industrial pollutant that has been linked to kidney disease.
The present work was designed for the investigation of the UPLC-QTOF–MS/MS
metabolite profile of the L. mucronate ethanolic extract (LME), along with
assessing the mechanistic protective actions of LME and its nano-silver formulation
(LMNS) against K2Cr2O7-induced nephrotoxicity in rats. LMNE was successfully
biosynthesized and confirmed using UV–Visible (UV–Vis) spectroscopy and
transmission electron microscopy (TEM). The nephroprotective effects of LME and
LMNE was assessed in rats exposed to potassium dichromate (K2Cr2O7, 15 mg/kg BW) to
cause nephrotoxicity. LME and LMNS, separately, were administered twice daily for
14 days at doses of 200 and 400 mg/kg BW, respectively. The kidney function,
catalase, UGT, Nrf2, PGE2, Cox-2, ERK, and MAPK levels in renal tissue were all
assessed, along with histopathological examinations for exploring their
ameliorative effects. Forty-five bioactive metabolites were annotated belonging to
flavonoids, phenolic and organic acids, coumarins, and fatty acids. Metabolite
profiling revealed that chlorogenic acid, apigenin, and luteolin glycosides were
the main phenolics, with chlorogenic acid-O-hexoside reported for the first time in
LME. The findings revealed that the serum kidney function indicators (urea and
creatinine) were markedly elevated in K2Cr2O7-intoxicated rats. Furthermore,
inflammatory indicators (COX-2 and PGE2), MAPK, and ERK were all markedly elevated
in kidney tissue, whereas catalase, UGT, and Nrf2 levels were downregulated.
Histological and immunohistochemical assays confirmed the toxic effects of K2Cr2O7
in the kidneys. In contrast, the administration of LME and LMNS prior to K2Cr2O7
considerably improved the architecture of the renal tissue, while also restoring
levels of most biochemical markers. Functioning via the inhibition of the MAPK/ERK
pathway, activating Nrf2, and modifying the antioxidant and metabolic enzymes, LME
and LMNS exerted their nephroprotective effects against K2Cr2O7-induced toxicity. ©
2023 by the authors.
AU - El-Fadaly, A. A.
AU - Younis, I. Y.
AU - Abdelhameed, M. F.
AU - Ahmed, Y. H.
AU - Ragab, T. I. M.
AU - El Gendy, A. E. N. G.
AU - Farag, M. A.
AU - Elshamy, A. I.
AU - Elgamal, A. M.
C7 - 786
DB - Scopus
DO - 10.3390/metabo13070786
IS - 7
KW - Launaea mucronata
oxidative stress
potassium dichromate toxicity
renal injury
secondary metabolites
UPLC-MS
apigenin
carboxylic acid
catalase
chlorogenic acid
coumaric acid
coumarin derivative
creatinine
cyclooxygenase 2
dichromate potassium
fatty acid
flavonoid
glucuronosyltransferase
launaea mucronata extract
luteolin
mitogen activated protein kinase
phenol derivative
plant extract
prostaglandin E2
unclassified drug
urea
animal experiment
animal model
animal tissue
Article
controlled study
drug formulation
drug toxicity
female
glycosylation
histopathology
kidney function
kidney tissue
LD50
male
metabolic fingerprinting
metabolite
mouse
nephrotoxicity
nonhuman
rat
renal protection
signal transduction
tandem mass spectrometry
ultra performance liquid chromatography
M3 - Article
PY - 2023
ST - Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-
Formulation against Nephrotoxicity in Rats as Revealed via Biochemical,
Histopathological, and UPLC-QTOF–MS/MS Analyses
T2 - Metabolites
TI - Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-
Histopathological, and UPLC-QTOF–MS/MS Analyses
85166289094&doi=10.3390%2fmetabo13070786&partnerID=40&md5=13cb7a5d1c284b96cd0318ade
9cce003
VL - 13
ID - 5019
ER -
TY - JOUR
AB - Plants belonging to the Launaea genus have been extensively utilized
ethnopharmacologically to treat a variety of diseases, including kidney disorders.
Chromium is a common industrial pollutant that has been linked to kidney disease.
The present work was designed for the investigation of the UPLC-QTOF-MS/MS
metabolite profile of the L. mucronate ethanolic extract (LME), along with
assessing the mechanistic protective actions of LME and its nano-silver formulation
(LMNS) against K2Cr2O7-induced nephrotoxicity in rats. LMNE was successfully
biosynthesized and confirmed using UV-Visible (UV-Vis) spectroscopy and
transmission electron microscopy (TEM). The nephroprotective effects of LME and
LMNE was assessed in rats exposed to potassium dichromate (K2Cr2O7, 15 mg/kg BW) to
cause nephrotoxicity. LME and LMNS, separately, were administered twice daily for
14 days at doses of 200 and 400 mg/kg BW, respectively. The kidney function,
catalase, UGT, Nrf2, PGE2, Cox-2, ERK, and MAPK levels in renal tissue were all
assessed, along with histopathological examinations for exploring their
ameliorative effects. Forty-five bioactive metabolites were annotated belonging to
flavonoids, phenolic and organic acids, coumarins, and fatty acids. Metabolite
profiling revealed that chlorogenic acid, apigenin, and luteolin glycosides were
the main phenolics, with chlorogenic acid-O-hexoside reported for the first time in
LME. The findings revealed that the serum kidney function indicators (urea and
creatinine) were markedly elevated in K2Cr2O7-intoxicated rats. Furthermore,
inflammatory indicators (COX-2 and PGE2), MAPK, and ERK were all markedly elevated
in kidney tissue, whereas catalase, UGT, and Nrf2 levels were downregulated.
Histological and immunohistochemical assays confirmed the toxic effects of K2Cr2O7
in the kidneys. In contrast, the administration of LME and LMNS prior to K2Cr2O7
considerably improved the architecture of the renal tissue, while also restoring
levels of most biochemical markers. Functioning via the inhibition of the MAPK/ERK
pathway, activating Nrf2, and modifying the antioxidant and metabolic enzymes, LME
and LMNS exerted their nephroprotective effects against K2Cr2O7-induced toxicity.
AN - WOS:001036603700001
AU - El-Fadaly, A. A.
AU - Younis, I. Y.
AU - Abdelhameed, M. F.
AU - Ahmed, Y. H.
AU - Ragab, T. I. M.
AU - El Gendy, A. G.
AU - Farag, M. A.
AU - Elshamy, A. I.
AU - Elgamal, A. M.
C7 - 786
DA - JUL
DO - 10.3390/metabo13070786
IS - 7
PY - 2023
SN - 2218-1989
ST - Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-
Histopathological, and UPLC-QTOF-MS/MS Analyses
T2 - METABOLITES
TI - Protective Action Mechanisms of Launaea mucronata Extract and Its Nano-
Histopathological, and UPLC-QTOF-MS/MS Analyses
VL - 13
ID - 6661
ER -
TY - JOUR
AB - To find potential alternatives for certain conventional antibiotics, we
investigated the effects of silver nanoparticles (AgNPs) synthesized using Moringa
oleifera extract on serum biochemistry, immunological, inflammatory, oxidative, and
histological alterations, and DNA damage in Oreochromis niloticus infected with
Aeromonas hydrophila. For determining the concentration of AgNPs, 110 fish were
used; 11 groups were challenged with Aeromonas hydrophila (each group = 10 fish)
and exposed to different concentrations of AgNPs (0, 0.4, 0.8, 1.2, 1.6, 2, 2.4,
2.8, 3.2, 3.6, and 4.0 mg/L) as immersion. Another 360 fish were categorized into
eight groups: G1 was non-infected with Aeromonas hydrophila and treated with 0 mg/L
AgNPs, G2 was infected and treated with 0 mg/L AgNPs, G3, G4, and G5 were non-
infected and treated with 0.4, 0.8, and 1.2 mg/L AgNPs, respectively. G6, G7, and
G8 were infected and treated with 0.4, 0.8, and 1.2 mg/L AgNPs, respectively, for
seven days. Infection with Aeromonas hydrophila significantly altered the health
and reduced lysozyme, hepatic CAT, and hepatic SOD activity, and IgM, complement 3,
total protein, albumin, globulin, serum nitric oxide, and IL-10 levels, with a
significant increase in the activity of ALT, AST, LDH, and levels of urea,
creatinine, bilirubin, hepatic MDA, and inflammatory biomarkers (IL-1β and IL-6).
Moreover, the infection produced a prominent genotoxic effect and impaired the
architecture of hepatic, renal, splenic, and gill tissues with a strong expression
of NF-kB. Fish exposed to different treatments of AgNPs showed improvement in these
markers with a significant reduction in the mortality rate. Interestingly, the
submersion of infected fish in 0.8 mg/L AgNPs produced the best result. These
results showed that green biosynthesis of AgNPs is non-cytotoxic and provides an
alternative antimicrobial compound against hepatotoxic, splenotoxic, nephrotoxic,
genotoxic, and immunosuppressive impacts of Aeromonas hydrophila in Nile tilapia. ©
2021 Elsevier B.V.
AU - El-Houseiny, W.
AU - Mansour, M. F.
AU - Mohamed, W. A. M.
AU - Al-Gabri, N. A.
AU - El-Sayed, A. A.
AU - Altohamy, D. E.
AU - Ibrahim, R. E.
C7 - 736430
DB - Scopus
DO - 10.1016/j.aquaculture.2021.736430
KW - Aeromonas hydrophila
Green synthesis
Immunity
Moringa oleifera
Nile tilapia
Nuclear factor-kappa B
antibiotics
apoptosis
bacterium
biochemistry
cichlid
genotoxicity
immune response
induced response
nanoparticle
oxidative stress
pollution effect
silver
M3 - Article
PY - 2021
ST - Silver nanoparticles mitigate Aeromonas hydrophila-induced immune
suppression, oxidative stress, and apoptotic and genotoxic effects in Oreochromis
niloticus
T2 - Aquaculture
TI - Silver nanoparticles mitigate Aeromonas hydrophila-induced immune
suppression, oxidative stress, and apoptotic and genotoxic effects in Oreochromis
niloticus
85100062381&doi=10.1016%2fj.aquaculture.2021.736430&partnerID=40&md5=cb9e23de371546
327b822d89ec1e0404
VL - 535
ID - 5202
ER -
TY - JOUR
AB - A novel anticancer and anti-inflammatory agent based on hybrid curcuminoid-
Gboxin analog (FLLL49-GbA) and its macromolecular silver(I) complex (Ag(I)FLLL49-
GbA) have successfully synthesized. In addition, chitosan nanoparticles (CNPs) were
used to encapsulate this macromolecular complex, targeting enhancing its
therapeutic effect and minimizing its side impacts. The encapsulated Ag(I) complex
was significantly triggered apoptosis (P < 0.05) with much more rapidly release of
Ag(I)FLLL49-GbA from the CNPs at pH 5.3 than at pH 7.4, which is beneficial for
cancer-targeted drug delivery. Free complex showed promising ability in preventing
glucose uptake and lactate production coupled with cellular ATP depletion in cancer
cells. Additionally, there was significant decrease in the inflammatory cytokines
in breast cancer (MCF-7) and lung cancer (A549) cells with values of P < 0.01 and P
< 0.001 after 24 h incubation. Furthermore, the death-inducing proteins have been
significantly up-regulated (P < 0.01 to P < 0.001) after 36 h incubation of cancer
cells. Consequently, the novel curcuminoid macromolecule showed significant
feasibility in triggering the high expression of apoptotic caspases caspase 3,
caspase 8, P53, and Bax (P < 0.01 to P < 0.001) after 48 h of chemotherapy.
Noteworthy, the cytotoxicity of Ag (I)FLLL49-GbA was significantly increased toward
cancer cells (MCF-7 > A549), while, reduced toward normal cells (HeLa) after
loading on chitosan Nano-vehicles. (C) 2020 Elsevier B.V. All rights reserved.
AN - WOS:000600773500108
AU - Elbehairi, S. E. I.
AU - Ismail, L. A.
AU - Alfaifi, M. Y.
AU - Elshaarawy, R. F. M.
AU - Hafez, H. S.
DA - DEC 15
DO - 10.1016/j.ijbiomac.2020.10.153
PY - 2020
SN - 0141-8130
1879-0003
SP - 2750-2764
ST - Chitosan nano-vehicles as biocompatible delivering tools for a new Ag(I)
curcuminoid-Gboxin analog complex in cancer and inflammation therapy
T2 - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
TI - Chitosan nano-vehicles as biocompatible delivering tools for a new Ag(I)
curcuminoid-Gboxin analog complex in cancer and inflammation therapy
VL - 165
ID - 6278
ER -
TY - JOUR
AB - A novel anticancer and anti-inflammatory agent based on hybrid curcuminoid-
Gboxin analog (FLLL49-GbA) and its macromolecular silver(I) complex (Ag(I)FLLL49-
GbA) have successfully synthesized. In addition, chitosan nanoparticles (CNPs) were
used to encapsulate this macromolecular complex, targeting enhancing its
therapeutic effect and minimizing its side impacts. The encapsulated Ag(I) complex
was significantly triggered apoptosis (P < 0.05) with much more rapidly release of
Ag(I)FLLL49-GbA from the CNPs at pH 5.3 than at pH 7.4, which is beneficial for
cancer-targeted drug delivery. Free complex showed promising ability in preventing
glucose uptake and lactate production coupled with cellular ATP depletion in cancer
cells. Additionally, there was significant decrease in the inflammatory cytokines
in breast cancer (MCF-7) and lung cancer (A549) cells with values of P < 0.01 and P
< 0.001 after 24 h incubation. Furthermore, the death-inducing proteins have been
significantly up-regulated (P < 0.01 to P < 0.001) after 36 h incubation of cancer
cells. Consequently, the novel curcuminoid macromolecule showed significant
feasibility in triggering the high expression of apoptotic caspases caspase 3,
caspase 8, P53, and Bax (P < 0.01 to P < 0.001) after 48 h of chemotherapy.
Noteworthy, the cytotoxicity of Ag(I)FLLL49-GbA was significantly increased toward
cancer cells (MCF-7 > A549), while, reduced toward normal cells (HeLa) after
loading on chitosan Nano-vehicles. © 2020 Elsevier B.V.
AU - Elbehairi, S. E. I.
AU - Ismail, L. A.
AU - Alfaifi, M. Y.
AU - Elshaarawy, R. F. M.
AU - Hafez, H. S.
DB - Scopus
DO - 10.1016/j.ijbiomac.2020.10.153
KW - Ag(I)-curcuminoid macromolecule-loaded chitosan nano-vehicles
Anticancer and anti-inflammatory
Structure-function relationship
A549 Cells
Caspase 3
Cell Proliferation
Cell Survival
Chitosan
Cytokines
Diarylheptanoids
HeLa Cells
Humans
MCF-7 Cells
Nanoparticles
Neoplasms
Silver
Up-Regulation
caspase 3
caspase 8
chitosan
curcuminoid derivative
death inducing protein
glucose
lactic acid
nanocarrier
protein Bax
protein p53
regulator protein
silver curcuminoid Gboxin analog complex
silver derivative
unclassified drug
CASP3 protein, human
cytokine
heptane derivative
nanoparticle
silver
A-549 cell line
apoptosis
Article
biocompatibility
cancer cell
cancer therapy
controlled study
drug cytotoxicity
drug effect
drug loading
drug release
drug structure
drug synthesis
drug targeting
encapsulation
feasibility study
glucose transport
HeLa cell line
human
human cell
incubation time
inflammation
malignant neoplasm
MCF-7 cell line
MTT assay
pH
pharmacological parameters
protein expression
upregulation
Western blotting
cell proliferation
cell survival
chemistry
metabolism
neoplasm
M3 - Article
PY - 2020
SP - 2750-2764
ST - Chitosan nano-vehicles as biocompatible delivering tools for a new
Ag(I)curcuminoid-Gboxin analog complex in cancer and inflammation therapy
TI - Chitosan nano-vehicles as biocompatible delivering tools for a new
Ag(I)curcuminoid-Gboxin analog complex in cancer and inflammation therapy
85094182312&doi=10.1016%2fj.ijbiomac.2020.10.153&partnerID=40&md5=607d4d9886a143d8a
c4773587f1b3f08
VL - 165
ID - 5270
ER -
TY - JOUR
AB - This study aimed to investigate the oxidative neurotoxicity induced by silver
nanoparticles (AgNPs) and assess the neuroprotective effects of quercetin against
this toxicity. Forty adult male rats were divided into four equal groups: control,
AgNPs (50 mg/kg intraperitoneally), quercetin (50 mg/kg orally), and quercetin +
AgNPs. After 30 days, blood and brain tissue samples were collected for further
studies. AgNP exposure increased lipid peroxidation and decreased glutathione
peroxidase, catalase, and superoxide dismutase activities in brain tissue. AgNPs
decreased serum acetylcholine esterase activity and γ-aminobutyric acid
concentrations. AgNPs upregulated tumor necrosis factor-α, interleukin-1β, and Bax
transcript levels. AgNPs reduced the transcripts of claudin-5, brain-derived
neurotrophic factor, paraoxonase, nuclear factor-erythroid factor 2 (Nrf2), and
Bcl-2. Histopathologically, AgNPs caused various degenerative changes and neuronal
necrosis associated with glial cell reactions. AgNPs increased the
immunohistochemical staining of glial fibrillary acidic protein (GFAP) in the
cerebrum and cerebellum. Oral treatment with quercetin efficiently counteracted the
opposing effects of AgNPs on brain tissue via modulation of tight junction
proteins, Nrf2, and paraoxonase, and its positive mechanism in modulating pro-
inflammatory cytokines and the downregulation of GFAP expression, and the apoptotic
pathway. AgNPs also altered the severity of histopathological lesions and modulated
GFAP immunostaining in the examined tissue. © 2022 by the authors. Licensee MDPI,
Basel, Switzerland.
AU - Elblehi, S. S.
AU - Abd El-Maksoud, E. M.
AU - Aldhahrani, A.
AU - Alotaibi, S. S.
AU - Ghamry, H. I.
AU - Elgendy, S. A.
AU - Soliman, M. M.
AU - Shukry, M.
C7 - 578
DB - Scopus
DO - 10.3390/life12040578
IS - 4
KW - brain
gene expression
neurotoxicity
quercetin
M3 - Article
PY - 2022
ST - Quercetin Abrogates Oxidative Neurotoxicity Induced by Silver Nanoparticles
in Wistar Rats
T2 - Life
TI - Quercetin Abrogates Oxidative Neurotoxicity Induced by Silver Nanoparticles
in Wistar Rats
85129023265&doi=10.3390%2flife12040578&partnerID=40&md5=dedc195473ef7ed24d0f69a190f
3474f
VL - 12
ID - 5115
ER -
TY - JOUR
AB - Silver nanoparticles have been used for numerous therapeutic purposes because
of their increased biodegradability and bioavailability, yet their toxicity remains
questionable as they are known to interact easily with biological systems because
of their small size. This study aimed to investigate and compare the effect of
silver nanoparticles’ particle size in terms of their potential hazard, as well as
their potential protective effect in an LPS-induced hepatotoxicity model. Liver
slices were obtained from Sprague Dawley adult male rats, and the thickness of the
slices was optimized to 150 µm. Under regulated physiological circumstances,
freshly cut liver slices were divided into six different groups; GP1: normal, GP2:
LPS (control), GP3: LPS + AgNpL (positive control), GP4: LPS + silymarin (standard
treatment), GP5: LPS + AgNpS + silymarin (treatment I), GP6: LPS + AgNpL +
silymarin (treatment II). After 24 h of incubation, the plates were gently removed,
and the supernatant and tissue homogenate were all collected and then subjected to
the following biochemical parameters: Cox2, NO, IL-6, and TNF-α. The LPS elicited
marked hepatic tissue injury manifested by elevated cytokines and proinflammatory
markers. Both small silver nanoparticles and large silver nanoparticles efficiently
attenuated LPS hepatotoxicity, mainly via preserving the cytokines’ level and
diminishing the inflammatory pathways. In conclusion, large silver nanoparticles
exhibited effective hepatoprotective capabilities over small silver nanoparticles.
AU - Elfaky, M. A.
AU - Sirwi, A.
AU - Ismail, S. H.
AU - Awad, H. H.
AU - Gad, S. S.
DB - Scopus
DO - 10.3390/cimb44070202
IS - 7
KW - cyclooxygenase enzyme 2
interleukin-6
nitric oxide
silymarin
cyclooxygenase 2
interleukin 6
lipopolysaccharide
silver nanoparticle
adult
animal tissue
Article
comparative study
controlled study
COX 2 gene
drug effect
drug efficacy
gene expression
IL 6 gene
liver injury
liver protection
male
NO gene
nonhuman
particle size
rat
risk assessment
TNF alpha gene
zeta potential
M3 - Article
PY - 2022
SP - 2923-2938
ST - Hepatoprotective Effect of Silver Nanoparticles at Two Different Particle
Sizes: Comparative Study with and without Silymarin
T2 - Current Issues in Molecular Biology
TI - Hepatoprotective Effect of Silver Nanoparticles at Two Different Particle
Sizes: Comparative Study with and without Silymarin
85133571531&doi=10.3390%2fcimb44070202&partnerID=40&md5=48f2c0625fd6d3355c8ef2f94ee
c1739
VL - 44
ID - 5069
ER -
TY - JOUR
AB - For the next-generation risk assessment (NGRA) of chemicals and
nanomaterials, new approach methodologies (NAMs) are needed for hazard assessment
in compliance with the 3R's to reduce, replace and refine animal experiments. This
study aimed to establish and characterize an advanced respiratory model consisting
of human epithelial bronchial BEAS-2B cells cultivated at the air-liquid interface
(ALI), both as monocultures and in cocultures with human endothelial EA.hy926
cells. The performance of the bronchial models was compared to a commonly used
alveolar model consisting of A549 in monoculture and in coculture with EA.hy926
cells. The cells were exposed at the ALI to nanosilver (NM-300K) in the VITROCELL
(R) Cloud. After 24 h, cellular viability (alamarBlue assay), inflammatory response
(enzyme-linked immunosorbent assay), DNA damage (enzyme-modified comet assay), and
chromosomal damage (cytokinesis-block micronucleus assay) were measured.
Cytotoxicity and genotoxicity induced by NM-300K were dependent on both the cell
types and model, where BEAS-2B in monocultures had the highest sensitivity in terms
of cell viability and DNA strand breaks. This study indicates that the four ALI
lung models have different sensitivities to NM-300K exposure and brings important
knowledge for the further development of advanced 3D respiratory in vitro models
for the most reliable human hazard assessment based on NAMs.
AN - WOS:000930353800001
AU - Elje, E.
AU - Mariussen, E.
AU - McFadden, E.
AU - Dusinska, M.
AU - Runden-Pran, E.
C7 - 407
DA - FEB
DO - 10.3390/nano13030407
IS - 3
PY - 2023
SN - 2079-4991
ST - Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture
Models for Hazard Assessment of Nanomaterials
T2 - NANOMATERIALS
TI - Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture
Models for Hazard Assessment of Nanomaterials
VL - 13
ID - 6725
ER -
TY - JOUR
AB - Ion-exchange resin beads, implanted into connective tissue, were used as the
vehicle for the delivery of radiolabelled cations to determine the local
distribution of lead, silver and cadmium in loose connective tissue. The system was
devoid of systemic toxicity, permitted a predictable release, enabled location of
released cations in respect to the site of bead implantation, afforded safety and
could be used with several other cations. The radiolabels released into the tissue
formed an immediate relationship with inflammatory cells and a more protracted
relationship with the matrix elements of the local connective tissue. The lesion
induced by the presence of the bead was complicated by the particular cation. ©
1988 Chapman and Hall Ltd.
AU - Ellender, G.
AU - Ham, K. N.
DB - Scopus
DO - 10.1097/00006231-198806000-00003
IS - 6
KW - Animal
Cadmium Radioisotopes
Connective Tissue
Ion Exchange Resins
Lead Radioisotopes
Radioisotopes
Rats
Silver
Tissue Distribution
cadmium 109
ion exchange resin
lead 210
radioisotope
silver 110m
unclassified drug
animal experiment
autoradiography
connective tissue
drug distribution
histology
nonhuman
rat
M3 - Article
PY - 1988
SP - 403-409
ST - Cationic radioisotope delivery to loose connective tissue in vivo using ion-
exchange resin beads
T2 - Nuclear Medicine Communications
TI - Cationic radioisotope delivery to loose connective tissue in vivo using ion-
exchange resin beads
0023794666&doi=10.1097%2f00006231-198806000-
00003&partnerID=40&md5=3320ba7412f40fd24c00c36b348fac14
VL - 9
ID - 5837
ER -
TY - JOUR
AB - The widespread use of silver in various forms raises concerns about its
potential adverse effects. Silver nanoparticles (AgNPs) can enter the brain and
subsequently induce neurotoxicity. As a source of diverse growth factors and for
its cytoprotective properties, platelet-rich plasma (PRP) has received considerable
attention in regenerative medicine. Our aim was to estimate the toxic effects of
AgNPs on the rat brain and assess the possible protective effects of PRP against
AgNPs induced neurotoxicity. A total of 40 adult male rats were divided into four
groups (n = 10), namely the control, AgNPs, AgNPs+PRP, and auto-recovery groups.
AgNPs were given intraperitoneally (i.p.) at a 10 mg/kg dose.bw daily for 28 days.
PRP was given (a day after AgNPs treatment) i.p. at a dose of 0.5 mL/kg.bw twice
weekly for 3 weeks. Rats in the auto-recovery group were left without treatment for
3 weeks after AgNP toxicity. Serum and brain tissue samples were collected for
assessment of proinflammatory cytokines, oxidative stress markers, as well as the
expression levels of apoptotic markers. Brain histopathological and
immunohistochemistry examinations were done. AgNPs significantly increased
oxidative stress markers and proinflammatory cytokines, decreased antioxidant
defense markers, and induced apoptosis and histopathological brain injuries.
However, PRP treatment restored brain oxidant/antioxidant balance, attenuated the
inflammatory state, prevented apoptosis, and improved the brain histopathological
lesions induced by AgNPs, with no significant improvements shown by auto-recovery
group. Our data provided a novel protective effect for PRP against AgNPs-induced
neurotoxicity due to its antioxidant, anti-inflammatory, and antiapoptotic effects.
© 2023 Wiley Periodicals LLC.
AU - Elmongy, N. F.
AU - Meawad, S. B.
AU - Elshora, S. Z.
AU - Atwa, A. H.
AU - Hammad, A. M.
AU - Mehanna, O. M.
AU - Ashry, W. M.
DB - Scopus
DO - 10.1002/jbt.23420
KW - brain toxicity
inflammation
neuronal apoptosis
platelet-rich plasma
M3 - Article
PY - 2023
ST - Platelet-rich plasma ameliorates neurotoxicity induced by silver
nanoparticles in male rats via modulation of apoptosis, inflammation, and oxidative
stress
T2 - Journal of Biochemical and Molecular Toxicology
TI - Platelet-rich plasma ameliorates neurotoxicity induced by silver
nanoparticles in male rats via modulation of apoptosis, inflammation, and oxidative
stress
85162659878&doi=10.1002%2fjbt.23420&partnerID=40&md5=b523422f33304616c48af154b7c41d
66
ID - 5037
ER -
TY - JOUR
AB - Periodontitis, as one of the most common diseases on a global scale, is a
public health concern. Microbial resistance to currently available antimicrobial
agents is becoming a growing issue in periodontal treatment. As a result, it is
critical to develop effective and environmentally friendly biomedical approaches to
overcome such challenges. The investigation of Streptomyces rochei MS-37’s
performance may be the first of its kind as a novel marine actinobacterium for the
green biosynthesis of silver nanoparticles (SNPs) and potentials as antibacterial,
anti-inflammatory, antibiofilm, and antioxidant candidates suppressing membrane-
associated dental infections. Streptomyces rochei MS-37, a new marine
actinobacterial strain, was used in this study for the biosynthesis of silver
nanoparticles for various biomedical applications. Surface plasmon resonance
spectroscopy showed a peak at 429 nm for the SNPs. The SNPs were spherical, tiny
(average 23.2 nm by TEM, 59.4 nm by DLS), very stable (−26 mV), and contained
capping agents. The minimum inhibitory concentrations of the SNPs that showed
potential antibacterial action ranged from 8 to 128 µg/mL. Periodontal pathogens
were used to perform qualitative evaluations of microbial adhesion and bacterial
penetration through guided tissue regeneration membranes. The findings suggested
that the presence of the SNPs could aid in the suppression of membrane-associated
infection. Furthermore, when the anti-inflammatory action of the SNPs was tested
using nitric oxide radical scavenging capacity and protein denaturation inhibition,
it was discovered that the SNPs were extremely efficient at scavenging nitric oxide
free radicals and had a strong anti-denaturation impact. The SNPs were found to be
more cytotoxic to CAL27 than to human peripheral blood mononuclear cells (PBMCs),
with IC50 values of 81.16 µg/mL in PBMCs and 34.03 µg/mL in CAL27. This study’s
findings open a new avenue for using marine actinobacteria for silver nanoparticle
biosynthesis, which holds great promise for a variety of biomedical applications,
in particular periodontal treatment. © 2022 by the authors.
AU - Elsilk, S. E.
AU - Khalil, M. A.
AU - Aboshady, T. A.
AU - Alsalmi, F. A.
AU - Ali, S. S.
C7 - 7296
DB - Scopus
DO - 10.3390/molecules27217296
IS - 21
antibacterial
antibiofilm
cytotoxicity
marine actinobacteria
Actinobacteria
Humans
Leukocytes, Mononuclear
Metal Nanoparticles
Plant Extracts
Silver
Streptomyces
antiinfective agent
metal nanoparticle
plant extract
silver
chemistry
human
metabolism
mononuclear cell
M3 - Article
PY - 2022
ST - Streptomyces rochei MS-37 as a Novel Marine Actinobacterium for Green
Biosynthesis of Silver Nanoparticles and Their Biomedical Applications
T2 - Molecules
TI - Streptomyces rochei MS-37 as a Novel Marine Actinobacterium for Green
Biosynthesis of Silver Nanoparticles and Their Biomedical Applications
85141581602&doi=10.3390%2fmolecules27217296&partnerID=40&md5=4bc33d5bed79110b59ea76
29d168d945
VL - 27
ID - 4961
ER -
TY - JOUR
AB - Thioacetamide (TAA) is one of the common fungicidal agents that induce liver
injury varying from inflammation, necrosis, and fibrosis to cirrhosis. Many recent
studies reported the beneficial effect of probiotics and silymarin on
hepatotoxicity regardless the causative agents. Therefore, the present study aimed
to evaluate the ameliorative role of probiotics and/or silymarin on TAA induced
hepatotoxicity in rats via histological, and immunohistochemical methods. Twenty
five male albino rats were used for this experiment and were divided into five
groups (n=5 rats/group); group I acts as negative control, group II was orally
administrated distilled water for six weeks, then injected with TAA (200 mg/kg
b.wt./ 5 ml physiological saline/ I.P.) twice a week for another six weeks, group
III was treated with probiotics at a dose of 135 mg/ kg b.wt. orally in drinking
water daily for six weeks, then injected with TAA (dosage of group II), twice
weekly for another six weeks, group IV was treated with silymarin at a dose of 200
mg/ kg b.wt orally 4 times per week for six weeks, then injected with TAA (dosage
of group II), twice weekly for another six weeks and group V was treated with
combination of both probiotics and silymarin, at the same dosage in groups III and
IV respectively then injected with TAA (dosage of group II), twice weekly for
another six weeks. Histologically, TAA induced hepatocytes degeneration,
inflammatory cells infiltration, and pseudolobular parenchyma as well as, high
apoptosis and low proliferation rates that were proved by immunohistochemical
staining for caspase 3 and ki-67 respectively. Probiotics and/or silymarin improved
the histological feature of hepatocytes, reduced apoptosis and stimulated
proliferation. Based on these results, we concluded that the use of probiotics and
silymarin combination ameliorates the hepatotoxic effect of TAA in rats more than
the use of probiotics or silymarin alone.
La tioacetamida (TAA) es uno de los agentes fungicidas más comunes que inducen
lesiones hepáticas que varían desde inflamación, necrosis y fibrosis hasta
cirrosis. Muchos estudios recientes informaron el efecto beneficioso de los
probióticos y la silimarina sobre la hepatotoxicidad independientemente de los
agentes causantes. Por lo tanto, el presente estudio tuvo como objetivo evaluar el
papel paliativo de los probióticos y / o silimarina en la hepatotoxicidad inducida
por TAA en ratas a través de métodos histológicos e inmunohistoquímicos. Para este
experimento se usaron veinticinco ratas albinas y se dividieron en cinco grupos (n
= 5 ratas / grupo); el grupo I se usó como control negativo; en el grupo II se
administró por vía oral agua destilada durante seis semanas y luego se inyectó TAA
(200 mg / kg b.wt./ 5 ml solución salina fisiológica / IP) dos veces por semana
durante otras seis semanas; el grupo III se trató con probióticos, dosis diaria de
135 mg / kg b.wt. por vía oral en agua potable, durante seis semanas y luego fue
inyectado con TAA (dosis del grupo II), dos veces por semana durante otras seis
semanas; el grupo IV se trató con silimarina, con una dosis de 200 mg / kg b.wt por
vía oral 4 veces por semana durante seis semanas, luego se inyectó TAA
(dosificación del grupo II), dos veces por semana durante otras seis semanas; y el
grupo V, se trató con una combinación de ambos probióticos y silimarina con la
misma dosis que en los grupos III y IV, respectivamente, luego fueron inyectados
con TAA (dosificación del grupo II), dos veces por semana durante otras seis
semanas. Histológicamente, la TAA indujo la degeneración de los hepatocitos, la
infiltración de células inflamatorias y el parénquima pseudolobular, así como
también una apoptosis alta y tasas de proliferación bajas que se probaron mediante
tinción inmunohistoquímica para caspasa 3 y ki-67, respectivamente. Los probióticos
y / o la silimarina mejoraron la característica histológica de los hepatocitos,
redujeron la apoptosis y estimularon la proliferación. En base a estos resultados,
concluimos que el uso de la combinación de probióticos y silimarina mejora el
efecto hepatotóxico del TAA en ratas más que el uso de probióticos o silimarina
individualmente.
AD - Emam, Mahmoud Abdelghaffar
Benha University. Faculty of Veterinary Medicine. Histology and Cytology
Department. EG
Farouk, Sameh Mohamed
Suez Canal University. Faculty of Veterinary Medicine. Cytology and Histology
Department. Ismailia. EG
Abdo, Mohamed
University of Sadat City. Faculty of Veterinary Medicine. Anatomy and Embryology
Department. EG
AU - Emam, Mahmoud Abdelghaffar
AU - Farouk, Sameh Mohamed
AU - Abdo, Mohamed
C1 - 20180706
DA - 2018/06
DB - LILACS
DO - 10.4067/S0717-95022018000200661
IS - 2
KW - Histology
Histología, Inmunohistoquímica
Hígado
Liver
Probiotics
Probióticos
Silimarina
Silymarin
Thioacetamide
Tioacetamida
LA - en
PY - 2018
SN - 0717-9367
SP - 661-669
ST - The ameliorative potential of probiotics and/or silymarin on thioacetamide
induced hepatotoxicity in rats: histological and immunohistochemical study
TI - The ameliorative potential of probiotics and/or silymarin on thioacetamide
induced hepatotoxicity in rats: histological and immunohistochemical study
TT - El potencial de mejora de los probióticos y / o silimarina sobre la
hepatotoxicidad inducida por tioacetamida en ratas: estudio histológico e
inmunohistoquímico
95022018000200661
VL - 36
ID - 4923
ER -
TY - JOUR
AB - The immune system contributes to maintaining the body's functional integrity
through its two main functions: recognizing and destroying foreign external agents
(invading microorganisms) and identifying and eliminating senescent cells and
damaged or abnormal endogenous entities (such as cellular debris or
misfolded/degraded proteins). Accordingly, the immune system can detect molecular
and cellular structures with a spatial resolution of a few nm, which allows for
detecting molecular patterns expressed in a great variety of pathogens, including
viral and bacterial proteins and bacterial nucleic acid sequences. Such patterns
are also expressed in abnormal cells. In this context, it is expected that
nanostructured materials in the size range of proteins, protein aggregates, and
viruses with different molecular coatings can engage in a sophisticated interaction
with the immune system. Nanoparticles can be recognized or passed undetected by the
immune system. Once detected, they can be tolerated or induce defensive
(inflammatory) or anti-inflammatory responses. This paper describes the different
modes of interaction between nanoparticles, especially inorganic nanoparticles, and
the immune system, especially the innate immune system. This perspective should
help to propose a set of selection rules for nanosafety-by-design and medical
nanoparticle design.
AN - WOS:000725696500001
AU - Ernst, L. M.
AU - Casals, E.
AU - Italiani, P.
AU - Boraschi, D.
AU - Puntes, V.
C7 - 2991
DA - NOV
DO - 10.3390/nano11112991
IS - 11
PY - 2021
SN - 2079-4991
ST - The Interactions between Nanoparticles and the Innate Immune System from a
Nanotechnologist Perspective
T2 - NANOMATERIALS
TI - The Interactions between Nanoparticles and the Innate Immune System from a
Nanotechnologist Perspective
VL - 11
ID - 6693
ER -
TY - JOUR
AB - Cytotoxic and mutagenic effects of root canal filling cements of various
chemical composition were determined in vitro. Materials set for 24 h and 1 wk were
eluted for 24 h in cell culture medium (cytotoxicity testing) and dimethyl
sulfoxide or physiological saline (mutagenicity testing). The differences between
cytotoxic potencies of eluates of the endodontic materials on L-929 cells were
quantified colorimetrically (MTT test). Eluates of Traitment SPAD were about 5-to
30-fold more toxic than silver-free AH26, Tubli-Seal, CRCS, and Endomethsone N. The
rank order of the toxic effects depended on the setting time of mixed materials.
Dimethyl sulfoxide and saline eluates of Traitment SPAD, Tubli-Seal, Endomethasone
N, CRCS, and Ketac-Endo were not mutagenic in the Ames test. Both eluates of
silver-free AH26 set for 24 h were weakly mutagenic in Salmonella typhimurium
TA100. Weak mutagenicity of saline eluates of the material was also observed in
TA97a and TA102. These results point to the possibility that mixed silver-free AH26
might contain small amounts of two mutagenic substances: bisphenol A diglycidyl
ether and formaldehyde. Copyright © 1999 by The American Association of
Endodontists.
AU - Ersev, H.
AU - Schmalz, G.
AU - Bayirli, G.
AU - Schweikl, H.
DB - Scopus
DO - 10.1016/S0099-2399(06)81172-6
IS - 5
KW - Administration, Topical
Animals
Bismuth
Calcium Hydroxide
Dexamethasone
Drug Combinations
Epoxy Resins
Formaldehyde
Hydrocortisone
L Cells (Cell Line)
Methenamine
Mice
Mutagenicity Tests
Polymers
Resorcinols
Salmonella typhimurium
Silver
Thymol
Titanium
Zinc Oxide
bismuth
Calcibiotic Root Canal Sealer
calcium hydroxide
dexamethasone
drug derivative
epoxy resin
formaldehyde
glass ionomer
hydrocortisone
Ketac Endo
methenamine
polymer
resorcinol derivative
silver
thymol
titanium
tubliseal
zinc oxide
zinc oxide eugenol
animal
article
chemistry
comparative study
drug combination
drug effect
L cell
mouse
mutagen testing
spad
M3 - Article
PY - 1999
SP - 359-363
ST - Cytotoxic and mutagenic potencies of various root canal filling materials in
eukaryotic and prokaryotic cells in vitro
TI - Cytotoxic and mutagenic potencies of various root canal filling materials in
eukaryotic and prokaryotic cells in vitro
0033128087&doi=10.1016%2fS0099-2399%2806%2981172-
6&partnerID=40&md5=59c075d06cf229654ec4dbea7b791e38
VL - 25
ID - 5762
ER -
TY - JOUR
AB - Zinc oxide (ZnO) nanoparticles (NPs) have received considerable attention in
the medical field because of their antibacterial properties, primarily for killing
and reducing the activity of numerous microorganisms. The purpose of this study was
to determine whether surface-modified ZnO NPs exhibit different properties compared
with unmodified ZnO. The antimicrobial and cytotoxic properties of modified ZnO NPs
as well as their effects on inflammatory cytokine production were evaluated. ZnO
NPs were prepared using a wet chemical method. Then, the surfaces of these NPs were
modified using 3-aminopropyltriethoxysilane (APTES) and dimethyl sulfoxide (DMSO)
as modifying agents via a chemical hydrolysis method. According to infrared
spectroscopy analysis (FTIR), the structure of the ZnO remained unchanged after
modification. Antibacterial assays demonstrated that APTES modification is more
effective at inducing an antimicrobial effect against Gram-negative bacteria than
against Gram-positive bacteria. Cytotoxicity studies showed that cell viability was
dose-dependent; moreover, pristine and APTES-modified ZnO exhibited low
cytotoxicity, whereas DMSO-modified ZnO exhibited toxicity even at a low NP
concentration. An investigation of inflammatory cytokine production demonstrated
that the extent of stimulation was related to the ZnO NP concentration but not to
the surface modification, except for IFN-gamma and IL-10, which were not detected
even at high NP concentrations. (C) 2016 Elsevier Ltd. All rights reserved.
AN - WOS:000387198300016
AU - Esparza-Gonzalez, S. C.
AU - Sanchez-Valdes, S.
AU - Ramirez-Barron, S. N.
AU - Loera-Arias, M. J.
AU - Bernal, J.
AU - Melendez-Ortiz, H. I.
AU - Betancourt-Galindo, R.
DA - DEC
DO - 10.1016/j.tiv.2016.09.020
PY - 2016
SN - 0887-2333
SP - 134-141
ST - Effects of different surface modifying agents on the cytotoxic and
antimicrobial properties of ZnO nanoparticles
TI - Effects of different surface modifying agents on the cytotoxic and
antimicrobial properties of ZnO nanoparticles
VL - 37
ID - 6573
ER -
TY - JOUR
AB - Background: Emerging data implicate nicotinamide phosphoribosyl transferase
(NAMPT) in the pathogenesis of cancer and inflammation. NAMPT inhibitors have
proven beneficial in inflammatory animal models of arthritis and endotoxic shock as
well as in autoimmune encephalitis. Given the role of inflammatory responses in
spinal cord injury (SCI), the effect of NAMPT inhibitors was examined in this
setting.Methods: We investigated the effects of the NAMPT inhibitor FK866 in an
experimental compression model of SCI.Results: Twenty-four hr following induction
of SCI, a significant functional deficit accompanied widespread edema,
demyelination, neuron loss and a substantial increase in TNF-α, IL-1β, PAR, NAMPT,
Bax, MPO activity, NF-κB activation, astrogliosis and microglial activation was
observed. Meanwhile, the expression of neurotrophins BDNF, GDNF, NT3 and anti-
apoptotic Bcl-2 decreased significantly. Treatment with FK866 (10 mg/kg), the best
known and characterized NAMPT inhibitor, at 1 h and 6 h after SCI rescued motor
function, preserved perilesional gray and white matter, restored anti-apoptotic and
neurotrophic factors, prevented the activation of neutrophils, microglia and
astrocytes and inhibited the elevation of NAMPT, PAR, TNF-α, IL-1β, Bax expression
and NF-κB activity.We show for the first time that FK866, a specific inhibitor of
NAMPT, administered after SCI, is capable of reducing the secondary inflammatory
injury and partly reduce permanent damage. We also show that NAMPT protein levels
are increased upon SCI in the perilesional area which can be corrected by
administration of FK866.Conclusions: Our findings suggest that the inflammatory
component associated to SCI is the primary target of these inhibitors. © 2012
Esposito et al; licensee BioMed Central Ltd.
AU - Esposito, E.
AU - Impellizzeri, D.
AU - Mazzon, E.
AU - Fakhfouri, G.
AU - Rahimian, R.
AU - Travelli, C.
AU - Tron, G. C.
AU - Genazzani, A. A.
AU - Cuzzocrea, S.
C7 - 554
DB - Scopus
DO - 10.1186/1742-2094-9-66
KW - Apoptosis
Cytokines
Inflammation
NAMPT inhibitor
Neurotrophic factors
Spinal cord injury
Acrylamides
Alcohol Oxidoreductases
Animals
Enzyme Inhibitors
Laminectomy
Male
Mice
Movement Disorders
Nerve Growth Factors
NF-kappa B
Nicotinamide Phosphoribosyltransferase
Peroxidase
Phosphorylation
Piperidines
Silver Staining
Spinal Cord
Spinal Cord Injuries
Time Factors
brain derived neurotrophic factor
daporinad
interleukin 1beta
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
nicotinamide phosphoribosyltransferase
protein Bax
serine
synaptotagmin I
acrylamide derivative
alcohol dehydrogenase
cytokine
enzyme inhibitor
N (4 (1 benzoylpiperidin 4 yl)butyl) 3 (pyridin 3 yl)acrylamide
N-(4-(1-benzoylpiperidin-4-yl)butyl)-3-(pyridin-3-yl)acrylamide
nerve growth factor
nerve protein
nicotinamide phosphoribosyltransferase, mouse
peroxidase
phenylacetaldehyde reductase
piperidine derivative
animal experiment
animal model
apoptosis
article
astrocyte
cell activation
controlled study
cytokine production
demyelination
disease severity
drug efficacy
enzyme activity
male
microglia
motor activity
motor performance
nonhuman
protein expression
spinal cord injury
treatment response
animal
disease model
drug effect
immunology
laminectomy
metabolism
motor dysfunction
mouse
nick end labeling
pathology
phosphorylation
silver staining
spinal cord
time
ultrastructure
M3 - Article
PY - 2012
ST - The NAMPT inhibitor FK866 reverts the damage in spinal cord injury
T2 - Journal of Neuroinflammation
TI - The NAMPT inhibitor FK866 reverts the damage in spinal cord injury
84859482714&doi=10.1186%2f1742-2094-9-
66&partnerID=40&md5=915f3fbcf36a49801be560b6ee2eb188
VL - 9
ID - 5717
ER -
TY - JOUR
AB - Resistance to therapy-mediated apoptosis in inflammatory breast cancer, an
aggressive and distinct subtype of breast cancer, was recently attributed to
increased superoxide dismutase (SOD) expression, glutathione (GSH) content, and
decreased accumulation of reactive species. In this study, we demonstrate the
unique ability of two Mn(III) N-substituted pyridylporphyrin (MnP)-based SOD mimics
(MnTE-2-PyP5+ and MnTnBuOE-2-PyP5+) to catalyze oxidation of ascorbate, leading to
the production of excessive levels of peroxide, and in turn cell death. The
accumulation of peroxide, as a consequence of MnP+ ascorbate treatment, was fully
reversed by the administration of exogenous catalase, showing that hydrogen
peroxide is essential for cell death. Cell death as a consequence of the action of
MnP + ascorbate corresponded to decreases in GSH levels, prosurvival signaling (p-
NF-kappa B, p-ERK1/2), and in expression of X-linked inhibitor of apoptosis
protein, the most potent caspase inhibitor. Although markers of classical apoptosis
were observed, including PARP cleavage and annexin V staining, administration of a
pan-caspase inhibitor, Q-VD-OPh, did not reverse the observed cytotoxicity. MnP +
ascorbate-treated cells showed nuclear translocation of apoptosis-inducing factor,
suggesting the possibility of a mechanism of caspase-independent cell death.
Pharmacological ascorbate has already shown promise in recently completed phase I
clinical trials, in which its oxidation and subsequent peroxide formation was
catalyzed by endogenous metalloproteihs. The catalysis of ascorbate oxidation by an
optimized metalbased catalyst (such as MnP) carries a large therapeutic potential
as an anticancer agent by itself or in combination with other modalities such as
radio- and chemotherapy. (C) 2013 Elsevier Inc. All rights reserved.
AN - WOS:000332429900031
AU - Evans, M. K.
AU - Tovmasyan, A.
AU - Batinic-Haberle, I.
AU - Devi, G. R.
DA - MAR
DO - 10.1016/j.freeradbiomed.2013.11.031
PY - 2014
SN - 0891-5849
1873-4596
SP - 302-314
ST - Mn porphyrin in combination with ascorbate acts as a pro-oxidant and mediates
caspase-independent cancer cell death
T2 - FREE RADICAL BIOLOGY AND MEDICINE
TI - Mn porphyrin in combination with ascorbate acts as a pro-oxidant and mediates
caspase-independent cancer cell death
VL - 68
ID - 6396
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely applied in various aspects of life.
However, recent studies reported their potential toxicity both on environment and
human health. The present study aimed to unravel the underlying molecular
mechanisms involved in AgNPs-induced brain toxicity. Moreover, chemopreventive
effect of tranilast, an analogue of tryptophan metabolite and a mast cell membrane
stabilizer was evaluated. Thirty Sprague Dawley rats were enrolled equally into
Normal control group, AgNPs-intoxicated group (50 mg/kg, 3 times/week) and
tranilast (300 mg/kg, 3 times/week)+AgNPs group. AgNPs administration triggered
brain oxidative stress as depicted by reduced Nrf-2 expression, decreased TAC and
GSH as well as upregulated brain lipid peroxidation. The apparent brain oxidative
damage was accompanied by elevated levels of inflammatory cytokines (IL-1β, IL-6
and TNF-α). Moreover, brain levels of TLR4, NLRP3 and caspase-1 were up-regulated.
Additionally, histological study indicated marked cellular injury in cerebrum and
cerebellum specimens. This was concomitant with elevated serum CK activity and CK-
BB level. On the other hand, tanilast administration remarkably alleviated AgNPs-
induced brain toxicity. The present study shed the light on implication of
TLR4/NLRP3 axis and NrF2 in AgNPs-induced brain toxicity. In addition, it explored
the potential protective effect of tranilast on AgNPs-induced brain injury via
antioxidant and anti-inflammatory efficacies. © 2020 Elsevier B.V.
AU - Fahmy, E. K.
AU - El-Sherbiny, M.
AU - Said, E.
AU - Elkattawy, H. A.
AU - Qushawy, M.
AU - Elsherbiny, N.
DB - Scopus
DO - 10.1016/j.neuro.2020.12.008
KW - Cerebral toxicity
NLRP3
Nrf-2
Tranilast
Animals
Caspase 1
Cerebrum
Creatine Kinase
Creatine Kinase, BB Form
Interleukin-1beta
Interleukin-6
Metal Nanoparticles
NF-E2-Related Factor 2
NLR Family, Pyrin Domain-Containing 3 Protein
ortho-Aminobenzoates
Oxidative Stress
Rats
Silver Compounds
caspase 3
CD68 antigen
creatine kinase
creatine kinase BB
cryopyrin
glutathione
interleukin 1beta
interleukin 1beta converting enzyme
interleukin 6
malonaldehyde
silver nanoparticle
tranilast
tryptophan
anthranilic acid derivative
metal nanoparticle
Nlrp3 protein, rat
silver derivative
Tlr4 protein, rat
adult
animal cell
animal experiment
animal model
animal tissue
Article
brain homogenate
brain injury
brain tissue
brain toxicity
cell damage
cerebellum
chemoprophylaxis
choroid plexus
controlled study
drug mechanism
enzyme activity
histopathology
in vivo study
lipid peroxidation
nerve cell necrosis
nonhuman
oxidative stress
priority journal
protein cleavage
protein expression
rat
Sprague Dawley rat
upregulation
animal
blood
drug effect
metabolism
pathology
M3 - Retracted
PY - 2021
SP - 167-176
ST - Tranilast ameliorated subchronic silver nanoparticles-induced cerebral
toxicity in rats: Effect on TLR4/NLRP3 and Nrf-2
T2 - NeuroToxicology
TI - Tranilast ameliorated subchronic silver nanoparticles-induced cerebral
toxicity in rats: Effect on TLR4/NLRP3 and Nrf-2
85098147543&doi=10.1016%2fj.neuro.2020.12.008&partnerID=40&md5=8d5801547e67e02df625
311cb63adaae
VL - 82
ID - 5280
ER -
TY - JOUR
AB - We presented a low-cost, eco-friendly, and efficient bacterium-mediated
synthesis of zinc oxide nanoparticles (ZnO-NPs) utilizing Paraclostridium
benzoelyticum strain 5610 as a capping and reducing agent. Scanning electron
microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, energy-
dispersive X-ray, and UV-vis spectroscopy were used to physiochemically
characterize the biosynthesized ZnO-NPs. A major narrow peak at 441 nm was observed
using UV-visible spectroscopy, verifying the presence of nanoparticles. According
to SEM and TEM studies, the average dimensions of ZnO-NPs was 50 nm. The crystal
size of 48.22 nm was determined by XRD analysis. FTIR analysis confirmed the
presence of various reducing metabolites on the surface of ZnO-NPs. The synthesized
nanoparticles were investigated for biological activity against Helicobacter suis,
Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis.
Helicobacter suis was the most vulnerable strain, with an inhibitory zone of 19.53
+/- 0.62 mm at 5 mg/mL dosage. The anti-inflammatory and the findings of the rat
paw edema experiments revealed that the bacterium-mediated ZnO-NPs had a strong
inhibitory action. In the arthritis model, the solution of ZnO-NPs showed 87.62 +/-
0.12% inhibitory effect of edema after 21 days when linked with that of the
standard drug. In the antidiabetic assay, ZnO-NPs sharply reduced glucose level in
STZ-induced diabetic mice. In this study, the particle biocompatibility by human
red blood cells was also determined. Keeping in view the biological importance of
ZnO-NPs, we may readily get the conclusion that Paraclostridium benzoelyticum
strain 5610-mediated ZnO-NPs will be a prospective antidiabetic, antibacterial,
antiarthritic, and anti-inflammatory agent in vivo experimental models and can be
used as a potent antidiabetic drug.
AN - WOS:000797681500002
AU - Faisal, S.
AU - Rizwan, M.
AU - Ullah, R.
AU - Alotaibi, A.
AU - Khattak, A.
AU - Bibi, N.
AU - Idrees, M.
C7 - 5994033
DA - MAY 5
DO - 10.1155/2022/5994033
PY - 2022
SN - 1942-0900
1942-0994
ST - Paraclostridium benzoelyticum Bacterium-Mediated Zinc Oxide Nanoparticles and
Their In Vivo Multiple Biological Applications
T2 - OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
TI - Paraclostridium benzoelyticum Bacterium-Mediated Zinc Oxide Nanoparticles and
Their In Vivo Multiple Biological Applications
VL - 2022
ID - 6594
ER -
TY - JOUR
AB - The current study aims to utilize the bacteria Paraclostridium benzoelyticum
strain 5610 to synthesize bio-genic silver nanoparticles (AgNPs). Biogenic AgNPs
were thoroughly examined using various characterization techniques such as UV-
spectroscopy, XRD, FTIR, SEM, and EDX. Synthesis of AgNPs was confirmed by UV–vis
analysis resulting in absorption peak at 448.31 nm wavelength. The SEM analysis
indicated the morphological characteristics and size of AgNPs which was 25.29 nm.
The face centered cubic (FCC) crystallographic structure was confirmed by XRD.
Furthermore, FTIR study affirmed the capping of AgNPs by different compounds found
in biomass of the Paraclostridium benzoelyticum strain 5610. Later, EDX was used to
determine the elemental composition with respective concentration and distribution.
Additionally, in the current study the antibacterial, anti-inflammatory,
antioxidant, anti-aging, and anti-cancer ability of AgNPs was assessed. The
antibacterial activity of AgNPs was tested against four distinct sinusitis
pathogens: Haemophilus in-fluenza, Streptococcus pyogenes, Moraxella catarrhalis
and Streptococcus pneumonia. AgNPs shows significant inhibition zone against
Streptococcus pyogenes 16.64 ± 0.35 followed by 14.32 ± 071 for Moraxella
catarrhalis. Similarly, the antioxidant potential was found maximum (68.37 ± 0.55%)
at 400 μg/mL and decrease (5.48 ± 0.65%) at 25 μg/mL, hence the significant
antioxidant ability was observed. Furthermore, anti-inflammatory activity of AgNPs
shows the strongest inhibitory action (42.68 ± 0.62%) for 15-LOX with lowest
inhibition activity for COX-2 (13.16 ± 0.46%). AgNPs have been shown to exhibit
significant inhibitory actions against the enzyme elastases AGEs (66.25 ± 0.49%),
which are followed by AGEs of visperlysine (63.27 ± 0.69%). Furthermore, the AgNPs
show high toxicity against HepG2 cell line which shows 53.543% reduction in the
cell viability after 24 h of treatment. The anti-inflammatory activity demonstrated
a potent inhibitory effect of the bio-inspired AgNPs. Overall, the biogenic AgNPs
have the ability to be served for the treatments of anti-aging and also due to
their anti-cancer, antioxidant abilities NPs may be a useful therapy choice for a
variety of disorders including cancer, bacterial infections and other inflammatory
diseases. Moreover, further studies are required in the future to evaluate their in
vivo biomedical applications. Highlights: Biogenic synthesis of AgNPs using
Paraclostridium benzoelyticum Strain for the first time. FTIR analysis confirmed
capping of potent biomolecules which are of great use in applied field especially
Nanomedicines. Notable antimicrobial activity against sinusitis bacteria and
cytotoxic potential of synthesized AgNPs on in vitro basis produce a new idea
shifting us to treat cancerous cell lines. © 2023 Wiley Periodicals LLC.
AU - Faisal, S.
AU - Ullah, R.
AU - Alotaibi, A.
AU - Zafar, S.
AU - Rizwan, M.
AU - Tariq, M. H.
DB - Scopus
DO - 10.1002/jemt.24362
IS - 7
KW - AgNPs
anti-aging
anti-cancer
anti-inflammatory
Paraclostridium benzoelyticum
Antioxidants
Bacteria
Glycation End Products, Advanced
Metal Nanoparticles
Plant Extracts
Silver
Cell culture
Diseases
Metal nanoparticles
Silver compounds
Ultraviolet spectroscopy
X ray diffraction
advanced glycation end product
antiinfective agent
antioxidant
metal nanoparticle
plant extract
silver
'current
AgNP
Anti-aging
Anti-cancer
Anti-inflammatories
Antibacterials
Biogenics
In-vitro
bacterium
chemistry
M3 - Article
PY - 2023
SP - 846-861
ST - Biofabrication of silver nanoparticles employing biomolecules of
Paraclostridium benzoelyticum strain: Its characterization and their in-vitro
antibacterial, anti-aging, anti-cancer and other biomedical applications
T2 - Microscopy Research and Technique
TI - Biofabrication of silver nanoparticles employing biomolecules of
Paraclostridium benzoelyticum strain: Its characterization and their in-vitro
antibacterial, anti-aging, anti-cancer and other biomedical applications
85160814913&doi=10.1002%2fjemt.24362&partnerID=40&md5=029b9468e7d6f4064cf4a40a9d5f3
3fa
VL - 86
ID - 5038
ER -
TY - JOUR
AB - Medicinal plants play a role in the traditional form of providing relief to
several diseases. Couroupita guianensis is also a type of medicinal plant which has
antibacterial, antimycobacterial, antimicrobial, antioxidant, antitumor, antiulcer,
antinociceptive, anthelmintic, antifertility, and antifungal activities. The
chemical constituent of C. guianensis such as indirubin serves as an antibacterial
and antifungal agent because it particularly cures fungal diseases. It is active
for the treatment of chronic myelocytic leukemia. The extract of isatin from the
flower of C. guianensis is also a chemical component that has been used as
prophylactic agent, prevents free radial-induced cancer, acts as a chemotherapeutic
agent to kill cancer cells, and it has antioxidant (act as major defense) and
anticancer activities against human promylocytic leukemia 60 cells. The extract of
chloroform of fruit of C. guianensis shows good antimicrobial activity but low
antimycobacterial activity. The plant extract of C. guianensis is equipotent to
standard drugs such as paracetamol in its analgesic activity and indomethacin in
its anti-inflammatory activity. © 2017 The Authors.
AU - Famina Jasmine, S.
AU - Vinayaga Moorthi, P.
DB - Scopus
DO - 10.22159/ajpcr.2017.v10i3.16174
IS - 3
KW - Anti biofil
Anti tumor activity
Antibacterial activity
Antioxidant activity
Couroupita quianensis
anthelmintic agent
antifungal agent
antiinfective agent
antimycobacterial agent
antioxidant
antiulcer agent
chloroform
contraceptive agent
Couroupita guianensis extract
fibroblast growth factor receptor 1
methanol
paracetamol
piperazine citrate
plant extract
silver nanoparticle
unclassified drug
Aedes aegypti
anthelmintic activity
antibiofilm ctivity
antifungal activity
antinociception
antiulcer activity
autophosphorylation
Bacillus subtilis
bacterium isolate
Candida albicans
CC50
contraception
Couroupita guianensis
cytotoxicity
drug activity
EC50
enzyme activity
enzyme inhibition
Ericales
Escherichia coli
human
leukocyte migration
Malassezia pachydermatis
Mycobacterium tuberculosis
nonhuman
Plesiomonas shigelloides
Proteus vulgaris
Review
Salmonella enterica serovar Typhi
Vibrio mimicus
wound healing
M3 - Review
PY - 2017
SP - 50-52
ST - Couroupita guianensis: The reservoir of medicinal compounds of human welfare
T2 - Asian Journal of Pharmaceutical and Clinical Research
TI - Couroupita guianensis: The reservoir of medicinal compounds of human welfare
85014766901&doi=10.22159%2fajpcr.2017.v10i3.16174&partnerID=40&md5=5fdfc5053862f9e4
75cdf9adf409a328
VL - 10
ID - 5513
ER -
TY - JOUR
AB - Neuroinflammation is a complex pathological process usually results from
abnormal microglial activation, thus, intervention in a microglial stimulation
pathway could be a promising approach for the treatment of neurode-generative
diseases. Luteolin is an important bioflavonoid possesses anti-inflammatory
properties, which is widely studied over these years. Light emitting diode (LED)
therapy is reported to be a potential therapeutic strategy for many diseases
including neurodegenerative diseases. However, little is known about the anti-
inflammatory effect of LED therapy on activated microglial cells, even less is
known whether there is a synergistic anti-inflammatory effect exist in LED and
luteolin therapy. In this study, we aimed to confirm the anti-inflammatory effect
of luteolin and LED combination therapy in lipopolysaccharide (LPS)-stimulated BV2
microglial cells. We showed that luteolin inhibited LPS-induced cytotoxicity, tumor
necrosis factor alpha (TNF alpha) and interleukin-6 (IL-6) production through
modulation of p38 and extracellular signal-regulated kinase (ERK) signaling in BV2
cells. In addition, LED therapy enhanced the anti-inflammatory effect of luteolin.
These results suggest that a synergistic effect between luteolin and LED could be a
new effective therapy in relieving neuroinflammation.
AN - WOS:000425213900025
AU - Fan, S. N.
AU - Habib, A.
AU - Liu, J.
AU - Tan, J.
IS - 1
PY - 2018
SN - 1943-8141
SP - 283-291
ST - LED enhances anti-inflammatoryeffect of luteolin (3', 4', 5,7-
tetrahydroxyflavone) in vitro
T2 - AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
TI - LED enhances anti-inflammatoryeffect of luteolin (3', 4', 5,7-
tetrahydroxyflavone) in vitro
VL - 10
ID - 6425
ER -
TY - JOUR
AB - Enterococcus faecalis (E. faecalis)is one of the dominant bacteria for
refractory infections of teeth. Silver ions (Ag+) have been proved to be a strong
bactericide but with high cytotoxicity and discoloration property. Simvastatin is
an agent used for dyslipidemia treatment and has anti-inflammatory property. In
this study, Ag(+)and simvastatin were for the first time used in combination, and
poly (lactide-co-glycolide) (PLGA) submicron particles carrying both Ag(+)and
simvastatin (AgS-PLGA) were fabricated for further investigations. Results
confirmed the enhanced antibacterial activity againstE. faecalisof Ag(+)by
simvastatin. AgS-PLGA could release both Ag(+)and simvastatin for 24 days and also
showed enhanced antibacterial activities. On dentin slices, AgS-PLGA could enter
dentinal tubules by ultrasonic activation and inhibit the colonization ofE.
faecalis. AgS-PLGA showed no cytotoxicity on MC3T3-E1 cells and slight suppressive
effect on RAW-264.7 cells, and could reduce the secretion of IL-6 and IL-1 beta of
RAW-264.7 cells. AgS-PLGA could be developed as a new biomaterial for infection and
inflammation control for dental and related medical treatments.
AN - WOS:000566619200001
AU - Fan, W.
AU - Duan, M. T.
AU - Sun, Q.
AU - Fan, B.
C6 - SEP 2020
DA - SEP 1
DO - 10.1080/09205063.2020.1811188
IS - 18
PY - 2020
SN - 0920-5063
1568-5624
SP - 2331-2346
ST - Simvastatin enhanced antimicrobial effect of Ag(+)againstE. faecalisinfection
of dentine through PLGA co-delivery submicron particles
T2 - JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
TI - Simvastatin enhanced antimicrobial effect of Ag(+)againstE. faecalisinfection
of dentine through PLGA co-delivery submicron particles
VL - 31
ID - 6418
ER -
TY - JOUR
AB - Enterococcus faecalis (E. faecalis) is one of the dominant bacteria for
refractory infections of teeth. Silver ions (Ag+) have been proved to be a strong
bactericide but with high cytotoxicity and discoloration property. Simvastatin is
an agent used for dyslipidemia treatment and has anti-inflammatory property. In
this study, Ag+ and simvastatin were for the first time used in combination, and
poly (lactide-co-glycolide) (PLGA) submicron particles carrying both Ag+ and
simvastatin (AgS-PLGA) were fabricated for further investigations. Results
confirmed the enhanced antibacterial activity against E. faecalis of Ag+ by
simvastatin. AgS-PLGA could release both Ag+ and simvastatin for 24 days and also
showed enhanced antibacterial activities. On dentin slices, AgS-PLGA could enter
dentinal tubules by ultrasonic activation and inhibit the colonization of E.
faecalis. AgS-PLGA showed no cytotoxicity on MC3T3-E1 cells and slight suppressive
effect on RAW-264.7 cells, and could reduce the secretion of IL-6 and IL-1β of RAW-
264.7 cells. AgS-PLGA could be developed as a new biomaterial for infection and
inflammation control for dental and related medical treatments. © 2020 Informa UK
AU - Fan, W.
AU - Duan, M.
AU - Sun, Q.
AU - Fan, B.
DB - Scopus
DO - 10.1080/09205063.2020.1811188
IS - 18
KW - antibacterial agent
E. faecalis
PLGA
silver
Simvastatin
Dentin
Silver
Bacteria
Cells
Metal ions
antiinfective agent
simvastatin
Ag +
Anti-microbial effects
Co deliveries
Property
Silver ions
Submicron particle
dentin
M3 - Article
PY - 2020
SP - 2331-2346
ST - Simvastatin enhanced antimicrobial effect of Ag+ against E. faecalis
infection of dentine through PLGA co-delivery submicron particles
T2 - Journal of Biomaterials Science, Polymer Edition
TI - Simvastatin enhanced antimicrobial effect of Ag+ against E. faecalis
infection of dentine through PLGA co-delivery submicron particles
85090240898&doi=10.1080%2f09205063.2020.1811188&partnerID=40&md5=4d84a809a9953d875a
f8a57b8ef5630b
VL - 31
ID - 5331
ER -
TY - JOUR
AB - Polyethylene glycol (PEG) is present in a variety of products. Little is
known regarding the accumulation of high molecular-weight PEGs or the long-term
effects resulting from PEG accumulation in certain tissues, especially the choroid
plexus. We evaluated the toxicity of high-molecular-weight PEGs administered to
Sprague Dawley rats. Groups of 12 rats per sex were administered subcutaneous
injections of 20, 40, or 60 kDa PEG or intravenous injections of 60 kDa PEG at 100
mg PEG/kg body weight/injection once a week for 24 weeks. A significant decrease in
triglycerides occurred in the 60 kDa PEG groups. PEG treatment led to a molecular-
weight-related increase in PEG in plasma and a low level of PEG in cerebrospinal
fluid. PEG was excreted in urine and feces, with a molecular-weight-related
decrease in the urinary excretion. A higher prevalence of anti-PEG IgM was observed
in PEG groups; anti-PEG IgG was not detected. PEG treatment produced a molecular-
weight-related increase in vacuolation in the spleen, lymph nodes, lungs, and
ovaries/testes, without an inflammatory response. Mast cell infiltration at the
application site was noted in all PEG-treated groups. These data indicate that
subcutaneous and intravenous exposure to high-molecular-weight PEGs produces anti-
PEG IgM antibody responses and tissue vacuolation without inflammation.
AN - WOS:000791286700003
AU - Fang, J. L.
AU - Vanlandingham, M. M.
AU - Beland, F. A.
AU - Felton, R. P.
AU - Maisha, M. P.
AU - Olson, G. R.
AU - Patton, R. E.
AU - Rosenberg, A. S.
AU - Da Costa, G. G. G.
DA - APR 15
DO - 10.1016/j.toxlet.2022.01.011
PY - 2022
SN - 0378-4274
1879-3169
SP - 22-30
ST - Toxicity of high-molecular-weight polyethylene glycols in Sprague Dawley rats
TI - Toxicity of high-molecular-weight polyethylene glycols in Sprague Dawley rats
VL - 359
ID - 6512
ER -
TY - JOUR
AB - Low efficiency of targeting and delivery toward the thrombus site poses
challenges to using thrombolytic drugs. Inspired by the biomimetic system of
platelet membranes (PMs) and glucose oxidase (GOx) modification technologies, we
develop a novel GOx-powered Janus nanomotor by asymmetrically attaching the GOx to
polymeric nanomotors coated with the PMs. Then the PM-coated nanomotors were
conjugated with urokinase plasminogen activators (uPAs) on their surfaces. The PM-
camouflaged design conferred excellent biocompatibility to the nanomotors and
improved their targeting ability to thrombus. The Janus distribution of GOx also
allows the uneven decomposition of glucose in biofluids to produce a chemophoretic
motion, increasing the drug delivery efficiency of nanomotors. In addition, these
nanomotors are located at the lesion site due to the mutual adhesion and
aggregation of platelet membranes. Furthermore, thrombolysis effects of nanomotors
are enhanced in static and dynamic thrombus as well as in mouse models. It is
believed that the novel PM-coated enzyme-powered nanomotors represent a great value
for thrombolysis treatment. © 2023 American Chemical Society.
AU - Fang, X.
AU - Ye, H.
AU - Shi, K.
AU - Wang, K.
AU - Huang, Y.
AU - Zhang, X.
AU - Pan, J.
DB - Scopus
IS - 7
KW - GOx
Janus
nanomotor
platelet
thrombus therapy
uPAs
Animals
Blood Platelets
Fibrinolytic Agents
Glucose Oxidase
Mice
Polymers
Thrombosis
Biocompatibility
Biomimetics
Blood vessels
Disease control
Efficiency
Glucose
Glucose sensors
Nanotechnology
Platelets
adenosine diphosphate
carrageenan
CD47 antigen
fibrinogen receptor
fibrinolytic agent
glucose oxidase
nanoparticle
PADGEM protein
sodium chloride
thrombin
urokinase
polymer
Nanomotors
Plasminogen activators
Platelet membranes
Targeted delivery
Thrombolysis
Thrombolytic
Thrombus therapy
Urokinase plasminogen
Urokinase plasminogen activator
animal cell
Article
biocompatibility
biosafety
blood clot lysis
blood toxicity
cell viability
coating (procedure)
conjugation
controlled study
cytotoxicity
decomposition
female
gel electrophoresis
HUVEC cell line
in vitro study
in vivo study
inflammatory cell
leukocyte count
lymphocyte count
monocyte
mouse
MTT assay
neutrophil count
nonhuman
protein content
protein fingerprinting
silver staining
surface property
thrombocyte membrane
thrombosis
TUNEL assay
Western blotting
animal
thrombocyte
Glucose oxidase
M3 - Article
PY - 2023
SP - 4302-4310
ST - GOx-Powered Janus Platelet Nanomotors for Targeted Delivery of Thrombolytic
Drugs in Treating Thrombotic Diseases
TI - GOx-Powered Janus Platelet Nanomotors for Targeted Delivery of Thrombolytic
Drugs in Treating Thrombotic Diseases
85163565601&doi=10.1021%2facsbiomaterials.3c00387&partnerID=40&md5=810554614467a448
6d276ff18099bc92
VL - 9
ID - 5003
ER -
TY - JOUR
AB - American Cutaneous Leishmaniasis (ACL) is a zoonosis caused by Leishmania
protozoa. The ACL chemotherapy available is unsatisfactory motivating researches to
seek alternative treatments. In this study, we investigated the action of biogenic
silver nanoparticle (AgNp-bio) obtained from Fusarium oxysporium, against
Leishmania amazonensis promastigote and amastigote forms. The AgNp-bio promastigote
treatment results in promastigote death leading to apoptosis-like events due an
increased production of reactive oxygen species (ROS), loss of mitochondrial
integrity, phosphatidylserine exposure and damage on promastigotes membrane. In L.
amazonensis infected macrophages, AgNp-bio treatment was still able to reduce the
percentage of infected macrophages and the amount of amastigotes per macrophage,
consequently, the amount of promastigotes recovered. This leishmanicidal effect was
also accompanied by a decrease in the levels of ROS and nitric oxide. By observing
the ultrastructural integrity of the intracellular amastigotes, we found that the
AgNp-bio treatment made a significant damage, suggesting that the compound has a
direct effect on intracellular amastigotes. These results demonstrated that AgNp-
bio had a direct effect against L. amazonensis forms and acted on immunomodulatory
ability of infected macrophages, reducing the infection without inducing the
synthesis of inflammatory mediators, which continuous stimulation can generate and
aggravate leishmaniotic lesions. Overall, our findings suggest that the use of
AgNp-bio stands out as a new therapeutic option to be considered for further in
vivo investigations representing a possible treatment for ACL. © 2017 Elsevier B.V.
AU - Fanti, J. R.
AU - Miranda-Sapla, M. M.
AU - Cataneo, A. H. D.
AU - Andrade, C. G. T. D. J.
AU - Panis, C.
AU - Rodrigues, J. H. D. S.
AU - Wowk, P. F.
AU - Kuczera, D.
AU - Costa, I. N.
AU - Nakamura, C. V.
AU - Nakazato, G.
AU - Durán, N.
DB - Scopus
DO - 10.1016/j.actatropica.2017.10.027
KW - Immunomodulation
Leishmaniasis
Nanoparticles
Nanotechnology
Reactive oxygen species (ROS)
Animals
Antiprotozoal Agents
Apoptosis
Leishmania
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Silver
Fusarium oxysporum
Leishmania amazonensis
Protozoa
nitric oxide
nitrite
phosphatidylserine
silver nanoparticle
antiprotozoal agent
metal nanoparticle
silver
apoptosis
cell organelle
immune system
nanoparticle
parasite
protist
reactive oxygen species
amastigote
animal cell
Article
cell damage
cell death
controlled study
death
dose response
drug activity
exposure
in vitro study
leishmanicidal effect
macrophage
membrane damage
mouse
necrosis
nonhuman
promastigote
zeta potential
animal
Bagg albino mouse
drug effect
leishmaniasis
M3 - Article
PY - 2018
SP - 46-54
ST - Biogenic silver nanoparticles inducing Leishmania amazonensis promastigote
and amastigote death in vitro
T2 - Acta Tropica
TI - Biogenic silver nanoparticles inducing Leishmania amazonensis promastigote
and amastigote death in vitro
85032457954&doi=10.1016%2fj.actatropica.2017.10.027&partnerID=40&md5=e09f0013b1fca2
2c2ba11bb7e44b89b3
VL - 178
ID - 5505
ER -
TY - JOUR
AB - Objective: Burn injuries are among the most common accidental health problems
worldwide, frequently leading to health and socio-economic challenges. Despite
this, no standard protocol for managing burn injuries can overcome the adverse
effects of currently used drugs. The present study sets out to develop and evaluate
the efficacy of new herbal ointments in providing synergistic anti-inflammatory,
antimicrobial, antioxidant, and cell-proliferating activities. It also investigates
the high-performance liquid chromatography (HPLC) characterisation of these new
herbal ointments. Method: Three different concentrations of the new herbal
ointment, which incorporates extracts of Matricaria aurea flower heads, arial parts
of Calendula tripterocarpa, Rosmarinus officinalis leaves, Alkanna tinctoria roots,
and myrrh were developed and evaluated. Ointments designed to promote burn-wound
healing were prepared and compared with β-sitosterol ointment and silver
sulfadiazine cream, as a commercial standards. Results: According to statistical
and histopathological analyses and visual inspections, the new herbal formulas
showed faster wound healing, more tolerability, and less toxicity than the
commercial standards. Conclusion: The new herbal ointments, developed in our study,
have shown promising results. The formula offers mechanical protection without any
release of non-biodegradable particles. It maintains the optimum moisture and pH of
the skin, while minimising scar-tissue formation. These advantages, in addition to
availability, low costs, and easy handling, may support the use of this new herbal
formula as an effective and safe alternative treatment, designed to promote the
healing of burn injuries. © 2020 The Authors
AU - Farhan, A.
AU - Alsuwayt, B.
AU - Alanazi, F.
AU - Yaseen, A.
AU - Ashour, M. A.
DB - Scopus
DO - 10.1016/j.jtumed.2020.10.023
IS - 2
KW - Alkanna tinctoria
Burn
Calendula tripterocarpa
Matricaria aurea
Myrrh
myrrh
plant extract
sulfadiazine silver
animal experiment
animal model
animal tissue
Article
burn
controlled study
flower head
male
nonhuman
ointment
plant leaf
plant root
rat
rosemary
M3 - Article
PY - 2021
SP - 152-161
ST - Evaluation and HPLC characterisation of a new herbal ointment for the
treatment of full-thickness burns in rats
T2 - Journal of Taibah University Medical Sciences
TI - Evaluation and HPLC characterisation of a new herbal ointment for the
treatment of full-thickness burns in rats
85098971557&doi=10.1016%2fj.jtumed.2020.10.023&partnerID=40&md5=2c9dc86653a32173bf6
e0700b608d884
VL - 16
ID - 5204
ER -
TY - JOUR
AB - Purpose: The main objective of this study is to investigate the antibacterial
activity of silver nanoparticles (AgNPs) against multidrug-resistant Salmonella
isolates recovered from diarrheic sheep and goats Methods: This study used chemical
reduction synthesis of AgNPs to evaluate their antimicrobial effects by estimation
of minimum inhibitory concentration (MIC) and minimum bactericidal concentration
(MBC) for each isolate using the microplate dilution method and tetrazolium salt
reduction test to detect the viability percentage. In vivo treatment efficacy was
assessed in mice by determining the viable count of Salmonella Enteritidis
recovered from feces and by hematologic, biochemical and histopathologic
examinations to confirm that use of AgNPs has no toxic or pathologic effects and to
evaluate its ability in tissue regeneration following treatment. Results: All
recovered strains were identified as MDR with a prevalence of 4% and 3.6% in sheep
and goats, respectively. The results of TEM, DLS, Zeta potential, and FTIR revealed
typical characteristics of the synthesized AgNPs. Silver nanoparticles showed
antibacterial activity against all recovered strains with MIC of <= 0.02 +/- 0.313
mu g/mL (mean average 0.085 +/- 0.126 mu g/mL) and MBC of 0.078 +/- 1.250 mu g/mL
(average 0.508 +/- 0.315 ng/mL). In vivo efficacy of AgNPs was observed by a
reduction in the number of viable S. Enteritidis recovered from feces in an S.
Enteritidis infected mouse model, with complete shedding stopping between treatment
days 4 and 6. Hematologic, serum biochemical, and histopathologic analyses proved
the ability of AgNPs to suppress inflammatory reaction caused by S. Enteritidis
infection. Conclusion: The study proved the effective ability of AgNPs to fight MDR
Salmonella spp. in vitro and in vivo without adverse effects.
AN - WOS:000575368600001
AU - Farouk, M. M.
AU - El-Molla, A.
AU - Salib, F. A.
AU - Soliman, Y. A.
AU - Shaalan, M.
DO - 10.2147/IJN.S270204
PY - 2020
SN - 1178-2013
SP - 6993-7011
ST - The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-
Resistant Salmonella Species Isolates
TI - The Role of Silver Nanoparticles in a Treatment Approach for Multidrug-
Resistant Salmonella Species Isolates
VL - 15
ID - 6042
ER -
TY - JOUR
AB - Liver ischemia-reperfusion injury (IRI) is a pathophysiological insult that
often occurs during liver surgery. Blackberry leaves are known for their anti-
inflammatory and antioxidant activities. Aims: To achieve site-specific delivery of
blackberry leaves extract (BBE) loaded AgNPs to the hepatocyte in IRI and to verify
possible molecular mechanisms. Methods: IRI was induced in male Wister rats. Liver
injury, hepatic histology, oxidative stress markers, hepatic expression of
apoptosis-related proteins were evaluated. Non-targeted metabolomics for chemical
characterization of blackberry leaves extract was performed. Key findings: Pre-
treatment with BBE protected against the deterioration caused by I/R, depicted by a
significant improvement of liver functions and structure, as well as reduction of
oxidative stress with a concomitant increase in antioxidants. Additionally, BBE
promoted phosphorylation of antiapoptotic proteins; PI3K, Akt and mTOR, while
apoptotic proteins; Bax, Casp-9 and cleaved Casp-3 expressions were decreased. LC-
HRMS-based metabolomics identified a range of metabolites, mainly flavonoids and
anthocyanins. Upon comprehensive virtual screening and molecular dynamics
simulation, the major annotated anthocyanins, cyanidin and pelargonidin glucosides,
were suggested to act as PLA2 inhibitors. Significance: BBE can ameliorate hepatic
IRI augmented by BBE-AgNPs nano-formulation via suppressing, oxidative stress and
apoptosis as well as stimulation of PI3K/Akt/mTOR signaling pathway. © 2023 by the
authors.
AU - Fathi, A. M.
AU - Waz, S.
AU - Alaaeldin, E.
AU - Toni, N. D. M.
AU - El-Sheikh, A. A. K.
AU - Sayed, A. M.
AU - Abdelmohsen, U. R.
AU - Nazmy, M. H.
C7 - 419
DB - Scopus
DO - 10.3390/metabo13030419
IS - 3
KW - AgNPs
blackberry
caspase-3
cell apoptosis
liver ischemia-reperfusion injury
metabolomics
PI3K/Akt/mTOR
anthocyanin
blackberry extract
caspase 3
caspase 9
catalase
cyanidin chloride
flavonoid
glucoside
glutathione
malonaldehyde
plant extract
protein Bax
protein bcl 2
quercetin
silver nanoparticle
silymarin
unclassified drug
urethan
Akt signaling
animal experiment
animal model
animal tissue
apoptosis
Article
cell survival
controlled study
cryopreservation
hepatic ischemia reperfusion injury
histopathology
hypoxia
immunoblotting
immunoreactivity
laparotomy
lipid peroxidation
liver function
liver histology
liver toxicity
male
molecular dynamics
MTT assay
nonhuman
oxidative stress
Pi3K/Akt signaling
protein expression
rat
TUNEL assay
upregulation
Western blotting
zeta potential
M3 - Article
PY - 2023
ST - Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via
PI3K/Akt/mTOR Pathway
T2 - Metabolites
TI - Blackberry-Loaded AgNPs Attenuate Hepatic Ischemia/Reperfusion Injury via
PI3K/Akt/mTOR Pathway
85151495875&doi=10.3390%2fmetabo13030419&partnerID=40&md5=d72994ba47651b732d21393c3
a06bfeb
VL - 13
ID - 5017
ER -
TY - JOUR
AB - Background: Overcoming the failure percentage of orthodontic mini-screws
(OMSs), which is about 30% of overall orthodontic cases, especially in malocclusion
treatment that requires orthopaedic heavy forces, is a great challenge. Bacterial
infections, soft tissue and bone inflammation, and weak connections between bones
and the OMS surface are among the main causalities of this failure. Objective: The
aim of the study is to evaluate in vitro the microbiological activities of the
deposited nanomaterials (Silver/hydroxyapatite nanoparticles (Ag/HA NPs) and zinc
oxide nanoparticles (ZnO NPs)) in terms of microbial inhibition. In addition, the
in-vitro cytotoxicity and cytocompatibility of the synthesized nano-coatings prior
to their in-vivo application in animal models were tested on four types of cells,
namely, fibroblasts, osteocytes, osteoblasts, and oral epithelial cells. Materials
and methods: Ag/HA NPs and ZnO NPs were built up onto the surface of titanium OMSs
by electrochemical deposition. This electrochemical deposition was performed on 50
orthodontic mini screws and the deposited materials were characterized with the aid
of scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX)
analysis, X-ray Diffraction (XRD) and nano-scratch test. In addition, the
microbiological activities of the deposited nanomaterials were explored in vitro in
terms of microbial inhibition. Furthermore, the cytotoxicity and cytocompatibility
were tested on four types of cells, namely, fibroblasts, osteocytes, osteoblasts
and oral epithelial cells. Results: SEM images revealed spherical Ag NPs in the
range of 40–70 nm in diameter, rod-shaped HA NPs and porous scaly ZnO NPs on the
surface of the OMSs. XRD analysis confirmed the crystal structures of AgNPs, HA
NPs, and ZnO NPs. ZnO NPs coated OMS had the highest antimicrobial activity than
Ag/HA coated OMS against Gram-positive, Gram-negative and fungal strains. Moreover,
after incubation, the decrease in the number of bacterial colonies was significant
with ZnO and Ag/HA nanoparticles (with the greatest decrease for the former), due
to the potent antibacterial effect of nanoparticles against Escherichia coli and
Enterococcus faecalis. Moreover, ZnO NPs-coated OMSs showed a better
cytocompatibility with oral epithelium, bone cells, and fibroblasts compared to
Ag/HA NPs. Conclusion: The suggested nanocoating is a promising strategy to
overcome the development of an inflammatory zone around the fixed OMSs. © 2022 The
Authors
AU - Fathy Abo-Elmahasen, M. M.
AU - Abo Dena, A. S.
AU - Zhran, M.
AU - Albohy, S. A. H.
C7 - 100711
DB - Scopus
DO - 10.1016/j.ortho.2022.100711
IS - 1
KW - Electrodeposition
Energy dispersive X-ray spectroscopy (SEM/EDX)
Hydroxyapatite
Nano-scratch test
Nanocoating technology
Orthodontic mini-screws
Silver Nanoparticles
TADS
X-ray Diffraction (XRD)
Zinc oxide
Animals
Durapatite
Inflammation
Silver
Titanium
Zinc Oxide
hydroxyapatite
silver
titanium
zinc oxide
animal
chemistry
inflammation
M3 - Article
PY - 2023
ST - Do silver/hydroxyapatite and zinc oxide nano-coatings improve inflammation
T2 - International Orthodontics
TI - Do silver/hydroxyapatite and zinc oxide nano-coatings improve inflammation
85143127447&doi=10.1016%2fj.ortho.2022.100711&partnerID=40&md5=69c1a7f2bb9f2897cada
b502bbfc542c
VL - 21
ID - 5031
ER -
TY - JOUR
AB - Hydraulic fracturing (“fracking”) is used in unconventional gas drilling to
allow for the free flow of natural gas from rock. Sand in fracking fluid is pumped
into the well bore under high pressure to enter and stabilize fissures in the rock.
In the process of manipulating the sand on site, respirable dust (fracking sand
dust, FSD) is generated. Inhalation of FSD is a potential hazard to workers
inasmuch as respirable crystalline silica causes silicosis, and levels of FSD at
drilling work sites have exceeded occupational exposure limits set by OSHA. In the
absence of any information about its potential toxicity, a comprehensive rat animal
model was designed to investigate the bioactivities of several FSDs in comparison
to MIN-U-SIL® 5, a respirable α-quartz reference dust used in previous animal
models of silicosis, in several organ systems (Fedan, J.S., Toxicol Appl Pharmacol.
00, 000–000, 2020). The present report, part of the larger investigation,
describes: 1) a comparison of the physico-chemical properties of nine FSDs,
collected at drilling sites, and MIN-U-SIL® 5, a reference silica dust, and 2) a
comparison of the pulmonary inflammatory responses to intratracheal instillation of
the nine FSDs and MIN-U-SIL® 5. Our findings indicate that, in many respects, the
physico-chemical characteristics, and the biological effects of the FSDs and MIN-U-
SIL® 5 after intratracheal instillation, have distinct differences. © 2020 Elsevier
Inc.
AU - Fedan, J. S.
AU - Hubbs, A. F.
AU - Barger, M.
AU - Schwegler-Berry, D.
AU - Friend, S. A.
AU - Leonard, S. S.
AU - Thompson, J. A.
AU - Jackson, M. C.
AU - Snawder, J. E.
AU - Dozier, A. K.
AU - Coyle, J.
AU - Kashon, M. L.
AU - Park, J. H.
AU - McKinney, W.
AU - Roberts, J. R.
C7 - 115282
DB - Scopus
DO - 10.1016/j.taap.2020.115282
KW - Fracking sand dust
MIN-U-SIL®
Particle characterization
Rat model
Silica
Air Pollutants, Occupational
Animals
Dust
Hydraulic Fracking
Inhalation Exposure
Lung
Male
Occupational Exposure
Pneumonia
Quartz
Rats
Sand
Silicon Dioxide
Silicosis
Trachea
aluminum
antimony
arsenic
barium
beryllium
cadmium
chromium
cobalt
copper
endotoxin
iron
lanthanum
lead
lithium
magnesium
manganese
phosphorus
silicon dioxide
silver
strontium
tellurium
thallium
tin
titanium
unindexed drug
vanadium
yttrium
zinc
zirconium
animal cell
animal experiment
animal model
animal tissue
Article
biological activity
cell hyperplasia
comparative study
controlled study
crystallization
dust
electron spin resonance
elemental analysis
enzyme activity
eosinophil
fracking
histiocytosis
histopathology
hypertrophy
inflammation
inhalation
lung alveolus epithelium cell
lung fibrosis
lung lavage
lung parenchyma
lung toxicity
lymphocyte
male
neutrophil
nonhuman
particle size
physical chemistry
rat
respirable particulate matter
sand
silicosis
X ray diffraction
adverse event
air pollutant
animal
chemistry
disease model
drug effect
exposure
lung
pneumonia
procedures
Sprague Dawley rat
trachea
M3 - Article
PY - 2020
ST - Biological effects of inhaled hydraulic fracturing sand dust. II. Particle
characterization and pulmonary effects 30 d following intratracheal instillation
TI - Biological effects of inhaled hydraulic fracturing sand dust. II. Particle
characterization and pulmonary effects 30 d following intratracheal instillation
85094567790&doi=10.1016%2fj.taap.2020.115282&partnerID=40&md5=b98965d8e36ec318b8b9b
bd37ce02853
VL - 409
ID - 5316
ER -
TY - JOUR
AB - Silica (SiO2) nanoparticles (NPs) have found extensive applications in
industrial manufacturing, biomedical and biotechnological fields. Therefore, the
increasing exposure to such ultrafine particles requires studies to characterize
their potential cytotoxic effects in order to provide exhaustive information to
assess the impact of nanomaterials on human health. The understanding of the
biological processes involved in the development and maintenance of a variety of
pathologies is improved by genome-wide approaches, and in this context, gene set
analysis has emerged as a fundamental tool for the interpretation of the results.
In this work we show how the use of a combination of gene-by-gene and gene set
analyses can enhance the interpretation of results of in vitro treatment of A549
cells with Ludox (R) colloidal amorphous silica nanoparticles. By gene-by-gene and
gene set analyses, we evidenced a specific cell response in relation to NPs size
and elapsed time after treatment, with the smaller NPs (SM30) having higher impact
on inflammatory and apoptosis processes than the bigger ones. Apoptotic process
appeared to be activated by the up-regulation of the initiator genes TNFa and IL1b
and by ATM. Moreover, our analyses evidenced that cell treatment with Ludox (R)
silica nanoparticles activated the matrix metalloproteinase genes MMP1, MMP10 and
MMP9. The information derived from this study can be informative about the
cytotoxicity of Ludox (R) and other similar colloidal amorphous silica NPs prepared
by solution processes.
AN - WOS:000342027500016
AU - Fede, C.
AU - Millino, C.
AU - Pacchioni, B.
AU - Celegato, B.
AU - Compagnin, C.
AU - Martini, P.
AU - Selvestrel, F.
AU - Mancin, F.
AU - Celotti, L.
AU - Lanfranchi, G.
AU - Mognato, M.
AU - Cagnin, S.
DA - SEP
DO - 10.3390/ijerph110908867
IS - 9
PY - 2014
SN - 1660-4601
SP - 8867-8890
ST - Altered Gene Transcription in Human Cells Treated with Ludox (R) Silica
Nanoparticles
T2 - INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
TI - Altered Gene Transcription in Human Cells Treated with Ludox (R) Silica
Nanoparticles
VL - 11
ID - 6356
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely known to have anti-inflammatory
properties, but the exact mechanism underlying this anti-inflammatory effect is not
clearly understood. Tumor necrosis factor-alpha (TNF alpha) is a major pro-
inflammatory cytokine that is expressed in the early stage of cell inflammation and
induces apoptosis by several known pathways. Our study aimed to investigate the
effect of AgNPs on the response of lung epithelial cells to TNF alpha and the
molecular mechanism of this response. Lung epithelial cell line NCI-H292 cells were
exposed to AgNPs (5 mu g/mL) and/or TNF alpha (20 ng/mL) for 24 h, then cellular
uptake was analyzed using flow cytometry. Our results showed that AgNPs were taken
up by cells in a dose-dependent manner and that the cellular uptake ratio of AgNPs
was significantly increased in the presence of TNF alpha. Apoptosis assays
indicated that exposure to AgNPs significantly decreased the apoptotic effect of
TNF alpha. Confocal microscopy was used to localize the tumor necrosis factor
receptor 1 (TNFR1) and revealed that TNFR1 localized on the surface of cells
exposed to TNF alpha. In contrast, TNFR1 localized inside cells exposed to both
AgNPs and TNF alpha, with very few receptors scattered on the cell membrane. The
results indicated that AgNPs reduced the cell surface TNFR1 expression level. The
results suggested that the reduction of surface TNFR1 reduced cellular response to
TNF alpha, resulting in an antiapoptotic effect. [GRAPHICS] .
AN - WOS:000438775300001
AU - Fehaid, A.
AU - Taniguchi, A.
DA - JUL 16
DO - 10.1080/14686996.2018.1487761
IS - 1
PY - 2018
SN - 1468-6996
1878-5514
SP - 526-534
ST - Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-
alpha
T2 - SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
TI - Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-
alpha
VL - 19
ID - 5857
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely known to have anti-inflammatory
properties, but the exact mechanism underlying this anti-inflammatory effect is not
clearly understood. Tumor necrosis factor-α (TNFα) is a major pro-inflammatory
cytokine that is expressed in the early stage of cell inflammation and induces
apoptosis by several known pathways. Our study aimed to investigate the effect of
AgNPs on the response of lung epithelial cells to TNFα and the molecular mechanism
of this response. Lung epithelial cell line NCI-H292 cells were exposed to AgNPs
(5 µg/mL) and/or TNFα (20 ng/mL) for 24 h, then cellular uptake was analyzed using
flow cytometry. Our results showed that AgNPs were taken up by cells in a dose-
dependent manner and that the cellular uptake ratio of AgNPs was significantly
increased in the presence of TNFα. Apoptosis assays indicated that exposure to
AgNPs significantly decreased the apoptotic effect of TNFα. Confocal microscopy was
used to localize the tumor necrosis factor receptor 1 (TNFR1) and revealed that
TNFR1 localized on the surface of cells exposed to TNFα. In contrast, TNFR1
localized inside cells exposed to both AgNPs and TNFα, with very few receptors
scattered on the cell membrane. The results indicated that AgNPs reduced the cell
surface TNFR1 expression level. The results suggested that the reduction of surface
TNFR1 reduced cellular response to TNFα, resulting in an anti-apoptotic effect. ©
2018, © 2018 The Author(s). Published by National Institute for Materials Science
in partnership with Taylor & Francis Group.
AU - Fehaid, A.
AU - Taniguchi, A.
DB - Scopus
DO - 10.1080/14686996.2018.1487761
IS - 1
KW - 211 Scaffold / Tissue engineering / Drug delivery
212 Surface and interfaces
30 Bio-inspired and biomedical materials
apoptosis
cellular uptake
lung cell
Biological organs
Cell culture
Cell death
Cell membranes
Glycoproteins
Macrophages
Metal nanoparticles
Scaffolds (biology)
Silver compounds
Tumors
211 Scaffold/Tissue engineering/Drug delivery
Biomedical material
Cellular uptake
Lung cells
Surface and interfaces
Tumor necrosis factors
Cell engineering
M3 - Article
PY - 2018
SP - 526-534
ST - Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α
T2 - Science and Technology of Advanced Materials
TI - Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α
85050085010&doi=10.1080%2f14686996.2018.1487761&partnerID=40&md5=a63d6c1e23a702adb6
dd674a756b0c79
VL - 19
ID - 5453
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely used in many consumer products due to
their anti-inflammatory properties. Therefore, the effect of exposure to AgNPs
should be investigated in diseased states in addition to healthy ones. Tumor
necrosis factor- (TNF) is a major cytokine that is highly expressed in many
diseased conditions, such as inflammatory diseases, sepsis, and cancer. We
investigated the effects of two different sizes of AgNPs on the TNF-induced DNA
damage response. Cells were exposed to 10 and 200 nm AgNPs separately and the
results showed that the 200 nm AgNPs had a lower cytotoxic effect with a higher
percent of cellular uptake compared to the 10 nm AgNPs. Moreover, analysis of
reactive oxygen species (ROS) generation and DNA damage indicated that TNF-induced
ROS-mediated DNA damage was reduced by 200 nm AgNPs, but not by 10 nm AgNPs. Tumor
necrosis factor receptor 1 (TNFR1) was localized on the cell surface after TNF
exposure with or without 10 nm AgNPs. In contrast, the expression of TNFR1 on the
cell surface was reduced by the 200 nm AgNPs. These results suggested that exposure
of cells to 200 nm AgNPs reduces the TNF-induced DNA damage response via reducing
the surface expression of TNFR1, thus reducing the signal transduction of TNF.
AN - WOS:000462542300037
AU - Fehaid, A.
AU - Taniguchi, A.
C7 - 1038
DA - MAR 1
DO - 10.3390/ijms20051038
IS - 5
PY - 2019
SN - 1422-0067
ST - Size-Dependent Effect of Silver Nanoparticles on the Tumor Necrosis Factor -
Induced DNA Damage Response
TI - Size-Dependent Effect of Silver Nanoparticles on the Tumor Necrosis Factor -
Induced DNA Damage Response
VL - 20
ID - 5968
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely used in many consumer products due to
their anti-inflammatory properties. Therefore, the effect of exposure to AgNPs
should be investigated in diseased states in addition to healthy ones. Tumor
necrosis factor-α (TNFα) is a major cytokine that is highly expressed in many
diseased conditions, such as inflammatory diseases, sepsis, and cancer. We
investigated the effects of two different sizes of AgNPs on the TNFα-induced DNA
damage response. Cells were exposed to 10 and 200 nm AgNPs separately and the
results showed that the 200 nm AgNPs had a lower cytotoxic effect with a higher
percent of cellular uptake compared to the 10 nm AgNPs. Moreover, analysis of
reactive oxygen species (ROS) generation and DNA damage indicated that TNFα-induced
ROS-mediated DNA damage was reduced by 200 nm AgNPs, but not by 10 nm AgNPs. Tumor
necrosis factor receptor 1 (TNFR1) was localized on the cell surface after TNFα
exposure with or without 10 nm AgNPs. In contrast, the expression of TNFR1 on the
cell surface was reduced by the 200 nm AgNPs. These results suggested that exposure
of cells to 200 nm AgNPs reduces the TNFα-induced DNA damage response via reducing
the surface expression of TNFR1, thus reducing the signal transduction of TNFα. ©
AU - Fehaid, A.
AU - Taniguchi, A.
C7 - 1038
DB - Scopus
DO - 10.3390/ijms20051038
IS - 5
KW - DNA damage
TNFR1
Tumor necrosis factor
Cell Line
Cell Survival
DNA Damage
Endocytosis
Humans
Immediate-Early Proteins
Metal Nanoparticles
Models, Biological
Particle Size
Receptors, Tumor Necrosis Factor, Type I
RNA, Messenger
Silver
Tumor Suppressor Proteins
ATM protein
checkpoint kinase 1
protein p53
rad 21 homolog
silver nanoparticle
structural maintenance of chromosomes 1a
tumor necrosis factor receptor 1
unclassified drug
BTG2 protein, human
immediate early protein
messenger RNA
metal nanoparticle
silver
tumor suppressor protein
antioxidant assay
Article
cell density
cell transport
cell viability
cell viability assay
controlled study
cytotoxicity
dichlorodihydrofluorescein assay
dispersity
DNA damage response
down regulation
flow cytometry
gene expression
genetic transfection
human
human cell
immunocytochemistry
immunofluorescence
light scattering
luciferase assay
oxidative stress
particle size
protein expression
upregulation
zeta potential
biological model
cell line
cell survival
chemistry
drug effect
endocytosis
genetics
metabolism
M3 - Article
PY - 2019
ST - Size-dependent effect of silver nanoparticles on the tumor necrosis factor α-
induced dna damage response
TI - Size-dependent effect of silver nanoparticles on the tumor necrosis factor α-
induced dna damage response
85062394902&doi=10.3390%2fijms20051038&partnerID=40&md5=be6d827ca09b5bf2a5547c992c1
7b273
VL - 20
ID - 5445
ER -
TY - JOUR
AB - BackgroundA link between histone deacetylases (HDACs) and intestinal
inflammation has been established. HDAC inhibitors that target gut-selective
inflammatory pathways represent a potential new therapeutic strategy in patients
with refractory inflammatory bowel diseases (IBD). AimsTo review the use of
selective HDAC inhibitors to treat gut inflammation and to highlight potential
improvements in selectivity/sensitivity by additional targeting of HDAC-regulating
microRNAs (miRNAs). MethodsOriginal articles and reviews have been identified using
PubMed search terms: histone deacetylase', HDAC inhibitor', inflammatory bowel
disease', gut inflammation,' and microRNA and HDAC'. ResultsThe use of butyrate in
distal colitis provided the first evidence that inhibition of HDACs decreases
intestinal inflammation in IBD. HDAC inhibitors, such as valproic acid, vorinostat
and givinostat, reduce inflammation and tissue damage in experimental murine
colitis. Potential mechanisms of action for HDAC inhibitors include increased
apoptosis, reduction of pro-inflammatory cytokine release, regulation of
transcription factors and modulation of HDAC-regulatory miRNAs. HDAC2, HDAC3,
HDAC6, HDAC9 and HDAC10 isoforms seem to be specifically involved in chronic
intestinal inflammation, justifying the use of selective inhibitors as new
therapeutic strategies in IBD. Controlling miRNAs for these isoforms can be
identified. ConclusionsThe pro-inflammatory influence of HDACs in the gut has been
confirmed, but mostly in murine studies. Considerably more human data are required
to permit development of selective HDAC inhibitors for IBD treatment. Inhibition of
key HDAC isoforms in combination with modulation of HDAC-regulatory miRNAs has
potential as a novel therapeutic approach.
AN - WOS:000346034200002
AU - Felice, C.
AU - Lewis, A.
AU - Armuzzi, A.
AU - Lindsay, J. O.
AU - Silver, A.
DA - JAN
DO - 10.1111/apt.13008
IS - 1
PY - 2015
SN - 0269-2813
1365-2036
SP - 26-38
ST - Review article: selective histone deacetylase isoforms as potential
therapeutic targets in inflammatory bowel diseases
T2 - ALIMENTARY PHARMACOLOGY & THERAPEUTICS
TI - Review article: selective histone deacetylase isoforms as potential
therapeutic targets in inflammatory bowel diseases
VL - 41
ID - 6539
ER -
TY - JOUR
AB - The over-use of antibiotics has promoted multidrug resistance and decreased
the efficacy of antibiotic therapy. Thus, it is still in great need to develop
efficient treatment strategies to combat the bacteria infection. The antimicrobial
photodynamic therapy (aPDT) and silver nanoparticles have been emerged as effective
antibacterial methods. However, the silver therapy may induce serious damages to
human cells at high concentrations and, the bare silver nanoparticles may rapidly
aggregate, which would reduce the antibacterial efficacy. The encapsulation of
sliver by nano-carrier is a promising way to avoid its aggregation and facilitates
the co-delivery of drugs for combination therapy, which does not require high
concentration of sliver to exert antibacterial efficacy. This work constructed a
self-assembled supermolecular nano-carrier consisting of the photosensitizers
(PSs), the anti-inflammatory agent and silver. The synthesized supermolecular nano-
carrier produced reactive oxygen species (ROS) under the exposure of 620-nm laser.
It exhibited satisfying biocompatibility in L02 cells. And, this nano-carrier
showed excellent antibacterial efficacy in Escherichia coli (E. coli) and
Staphylococcus aureus (S. aureus) as indicated by bacterial growth and colony
formation. Its antibacterial performance is further validated by the bacteria
morphology through the scanning electron microscope (SEM), showing severely damaged
structures of bacteria. To summary, the supermolecular nano-carrier TCPP-MTX-Ag-NP
combining the therapeutic effects of ROS and silver may serve as a novel strategy
of treatment for bacterial infection. © Copyright © 2021 Feng, Huang, Jiang, Deng,
Li, Li, Wu, Li, Sun, Huang, Qin, Liang and Fu.
AU - Feng, G. N.
AU - Huang, X. T.
AU - Jiang, X. L.
AU - Deng, T. W.
AU - Li, Q. X.
AU - Li, J. X.
AU - Wu, Q. N.
AU - Li, S. P.
AU - Sun, X. Q.
AU - Huang, Y. G.
AU - Qin, A. P.
AU - Liang, L.
AU - Fu, J. J.
C7 - 666408
DB - Scopus
DO - 10.3389/fchem.2021.666408
KW - antimicrobial photodynamic therapy
bacteria
silver
supermolecular nano-carrier
synergistic efficacy
M3 - Article
PY - 2021
ST - The Antibacterial Effects of Supermolecular Nano-Carriers by Combination of
Silver and Photodynamic Therapy
T2 - Frontiers in Chemistry
TI - The Antibacterial Effects of Supermolecular Nano-Carriers by Combination of
Silver and Photodynamic Therapy
85105021593&doi=10.3389%2ffchem.2021.666408&partnerID=40&md5=9fe10eaad3bcc389487a0a
bda7901982
VL - 9
ID - 5236
ER -
TY - GEN
AB - O agregado de trióxido mineral (MTA) tem sido amplamente utilizado em
Endodontia, devido à sua boa adaptação marginal e biocompatibilidade. Na tentativa
de associar propriedades físico-químicas de um cimento obturador de canal radicular
a biocompatibilidade do MTA, algumas modificações foram feitas no MTA para
possibilitar seu uso como um cimento obturador de canal radicular. Assim, a reação
de promovida por um cimento a base de MTA (MTA Fillapex) em subcutâneo de ratos foi
investigada por análise morfológica e morfométrica. Oitenta ratos foram
distribuídos em 4 grupos (n=20); em cada animal, um tubos de polietileno preenchido
com MTA Fillapex, MTA, AH Plus ou Fill Canal foi implantado no subcutânea dorsal.
Após 7, 15, 30 e 60 dias, os tubos rodeados por tecido conjuntivo foram removidos,
fixados em formaldeído 4% e incluídos em parafina. Nos cortes corados com HE, foi
avaliada a densidade numérica de células inflamatórias na cápsula e análises
estatísticas realizadas usando ANOVA e do Student-Newman-Keuls (p≤0.05). Alguns
cortes foram submetidos a imuno-histoquímica para marcação de macrófagos; o método
de von Kossa foi usado para detecção de estruturas calcificadas. Nossos resultados
revelaram que, nos períodos de 7 para 15 dias, o número de células inflamatórias
foi significantemente mais elevado na cápsula do MTA Fillapex em comparação com
outros materiais. No entanto, uma redução significante na densidade numérica de
células inflamatórias foi verificada na cápsula do MTA Fillapex aos 30 e 60 dias
quando comparado com os períodos iniciais. Aos 30 dias, macrófagos imunopositivos
foram observados principalmente na superfície da cápsula em íntima justaposição ao
MTA Fillapex, MTA e AH Plus. No entanto, o intenso processo inflamatório crônico
adjacente ao Fill Canal exibiu numerosas células imunopositivas por toda extensão
da cápsula. No período de 60 dias, a reação inflamatória promovida pelo MTA
Fillapex foi semelhante do MTA e significantemente menor ao Fill Canal de
preenchimento. Em contrapartida, observou-se um aumento gradual e significante de
células inflamatórias na cápsula adjacente ao Fill Canal. Estruturas von Kossa-
positivas foram observadas na cápsula adjacente para o MTA Fillapex, o MTA e AH
Plus; estruturas positivas não foram encontradas na cápsula ao redor do Fill Canal.
Os nossos resultados indicam que MTA Fillapex apresente, no período de 60 dias,
biocompatibilidade semelhante ao MTA
Mineral Trioxide Aggregate (MTA) has been widely used in Endodontics due to its
good marginal adaptation and biocompatibility. In attempt to associate
physicochemical properties of a root canal sealer with biocompatibility of the MTA,
some modifications have been made in the MTA to provide its use as a root canal
sealer. Thus, the tissue reaction promoted by a MTA-based sealer (MTA-Fillapex) in
rat subcutaneous was investigated by morphological and morphometric analyses.
Eighty male rats were distributed into 4 groups (n=20); in each animal, one
polyethylene tubes filled with MTA-Fillapex, MTA, AH-Plus or Fill Canal was
implanted in the dorsal subcutaneous. After 7, 15, 30 and 60 days, the tubes
surrounded by connective tissue were removed, fixed in 4% formaldehyde and embedded
in paraffin. In the HE-stained sections, the numerical density of inflammatory
cells in the capsule was evaluated and statistical analyses performed using ANOVA
and Student-Newman-Keuls test (p≤0.05). Sections were submitted to
immunohistochemistry for the detection of macrophage; von Kossa method was used for
detection of calcified structures. Our results revealed that, in the periods of 7
and 15 days, the number of inflammatory cells was significantly higher in the
capsule of the MTA-Fillapex in comparison to other materials. However, significant
reduction in the density numerical of inflammatory cells was verified in the
capsule of the MTA-Fillapex in the 30 and 60 days when compared to the initial
periods. At 30 days, immunolabelling for macrophages was mainly detected in the
surface of capsule in close juxtaposition to the MTA Fillapex, MTA and AH Plus.
However, the intense chronic inflammatory process adjacent to the Fill Canal
exhibited numerous immunolabelled cells. At 60 days, the inflammatory reaction
promoted by MTA Fillapex was similar to the MTA and significantly lower than Fill
Canal. Otherwise, a gradual and significant increase was observed in the number of
inflammatory cells in the capsule adjacent to the Fill Canal. Structures von
Kossapositive were observed in the capsule adjacent to the MTA-Fillapex, MTA and
AHPlus; positive structures were not seen in the capsule surrounding the Fill
Canal. In the period of 60 days, our results indicate that MTA-Fillapex, exhibits
biocompatibility similar to MTA
AU - Ferino, Rafael Vicente
C1 - 20140407
C8 - biblio-866842
DA - 2012/00
DB - LILACS
KW - Endodontia
Endodontics
Imunoistoquímica
Material testing
Ratos
Rats
Teste de materiais
LA - pt
PY - 2012
SP - 60-60
ST - Reação histológica ao cimento endodôntico à base de MTA (Fillapex®) em
subcutâneo de ratos
TI - Reação histológica ao cimento endodôntico à base de MTA (Fillapex®) em
subcutâneo de ratos
TT - Histological reaction to the endodontic MTA-based sealer (Fillapex®) in rat
subcutaneous
UR -
https://repositorio.unesp.br/bitstream/handle/11449/90405/ferino_rv_me_arafo.pdf?
sequence=1&isAllowed=y
ID - 4946
ER -
TY - JOUR
AB - Stryphnodendron adstringens (Martius) Coville is a medicinal plant described
as having pharmacological properties as anti-inflammatory and antimicrobial
activities. Silver chloride nanoparticles (AgCl-NPs) have shown great potential for
biomedical applications with efficient antimicrobial properties. Here, we report
the photosynthesis of AgCl-NPs using plant extract from S. adstringens (SaAgCl-NPs)
and their cytotoxic and antimicrobial activities. We photosynthesized SaAgCl-NPs
nearly spherical with low heterogeneity in size and enveloped by an organic
material layer responsible for colloidal stability. SaAgCl-NPs was non-cytotoxic
against mammalian VERO cells; however, SaAgCl-NPs presented remarkable antifungal
activity against the pathogenic yeast Cryptococcus neoformans (MIC80 of 0.32 mu
g/mL) the causative agent of human cryptococcosis. Notable antibacterial activity
was observed against Gram-negative bacteria Pseudomonas aeruginosa (MIC80 of 2.56
mu g/mL) e Serratia marcescens (MIC80 of 20.48 mu g/mL) both microorganisms
associated with a variety of human infections, in particular pneumonia. In
contrast, Gram-positive bacteria Staphylococcus aureus and Staphylococcus
epidermidis, microorganisms that cause pathologies as skin infections, were less
susceptible to the SaAgCl-NPs both with MIC80 of 40.93 mu g/mL. Thus, SaAgCl-NPs
represents an organic-inorganic hybrid nanomaterial with very low cytotoxicity
against mammalian cells and high antimicrobial efficiency against pathogenic
microorganisms and may be explored as an alternative to antimicrobial drugs.
AN - WOS:000615190900002
AU - Fernandes, D. G. D.
AU - Andrade, V. B.
AU - Lucena, L. N.
AU - Ambrosio, F. N.
AU - de Souza, A. L. M.
AU - Batista, B. L.
AU - Rolim, W. R.
AU - Seabra, A. B.
AU - Lombello, C. B.
AU - da Silva, F. D.
AU - Garcia, W.
C6 - FEB 2021
DA - MAR
DO - 10.1007/s10876-021-02011-w
IS - 2
PY - 2022
SN - 1040-7278
1572-8862
SP - 687-695
ST - Cytotoxicity and Antimicrobial Properties of Photosynthesized Silver Chloride
Coville
TI - Cytotoxicity and Antimicrobial Properties of Photosynthesized Silver Chloride
Coville
VL - 33
ID - 6230
ER -
TY - GEN
AB - A raiva é uma doença do sistema nervoso central que é quase invariavelmente
fatal. Apesar de causar cerca de 60.000 mortes/ano, a raiva ainda permanece uma
doença negligenciada na maioria dos países, principalmente naqueles em
desenvolvimento. O objetivo do nosso estudo foi verificar nos microambientes
meningeal, perivascular e intraparenquimatoso do sistema nervoso central, o
processo inflamatório, a resposta imune do hospedeiro e a morte dos neurônios
frente à infecção rábica transmitida por morcegos. Verificamos que a raiva humana
transmitida por morcegos é uma meningoencefalomielite. Através de reação imuno-
histoquímica caracterizamos e quantificamos in situ a distribuição do antígeno
viral, o fenótipo de células inflamatórias, as células expressando citocinas pró
inflamatórias, citocinas representativas de padrão Th1 e Th2 e células em apoptose.
O antígeno viral foi encontrado difusamente no parênquima cerebral, em maior
abundância em neurônios, não diferindo sua distribuição em relação as regiões
cerebrais. As células da glia, em especial os astrócitos, estavam imunomarcadas com
o antígeno da raiva, assim como as células endoteliais e células mononucleadas da
luz vascular. Esses achados contribuíram para a hipótese da ocorrência de uma via
hematogênica alternativa de disseminação viral, através da infecção de células
endoteliais pelo vírus, posterior infecção de astrócitos e finalmente infecção de
neurônios. A expressão significativa de IL-12 nos pacientes rábicos provavelmente
traduz imunidade de base preservada. Identificamos, outrossim, falta de resposta
efetiva das células NK e comprometimento da resposta imune adaptativa sequencial,
demonstrada pela depleção de linfócitos TCD4+ no parênquima cerebral, prejuízo da
atividade citotóxica dos linfócitos TCD8+ com proporcionalmente baixa expressão de
granzima e expressão rarefeita de IFN-g e IL-2r. Essa situação deve ser reflexo da
manipulação do vírus sobre a resposta imune inata e adaptativa...
Viral disease of central nervous system almost invariable fatal, rabies causes
about 60.000 deaths yearly, and still remain a neglected disease in most countries,
specially in developing ones. We study the meningeal, perivascular and parenquimal
environment in central nervous system from patients who dies after bat transmitted
rabies, looking for the inflammatory process, host immune response and neuronal
death. We show that human rabies transmitted by bats is a meningoencephalomyelitis.
Immunohistochemistry allows the in situ quantification of viral antigen
distribution, inflammatory cellular phenotype, expression of cytokines representing
both Th1 and Th2 profile and pro-inflammatory and cell apoptosis. Viral antigen was
found disseminatted in cerebral parenquima, more abundant in neurons, without
cerebral area preference. Glial cells, specially astrocytes, were immunostained
with rabies antigen, as well as endothelial and vascular mononuclear cells. These
findings contributed for an alternative hypothesis of the occurrence of hematogenic
viral dissemination, through viral infection or passage by endothelial cells,
followed by astrocyte infection, and finally reaching neurons. IL-12 significant
expression in central nervous system from rabies patients probably reflect
preservation of immunity. The lack of effective NK cells could compromise adaptive
immune response, demonstred by TCD4+ lymphocytes depletion in cerebral parenquima,
ineffective TCD8+ cytotoxic activity with low granzyme expression and low
expression of IFN-g and IL-2r. This situation reflects viral effect on host innate
and adaptive immune response leading to an ineffective response for viral
clearance. High pro-inflammatory cytokine expression, IL- 1b, IL-6 and TNF-a,
contribute for neuronal damage, with predominant Th2 cytokine profile in central
nervous system in rabies patients, with high expression of IL-4 and IL-10.
Increased TGF-b expression also shows an immune suppressor...
AU - Fernandes, Elaine Raniero
C1 - 20110323
C8 - lil-587148
DA - 2009/00
DB - LILACS
KW - Central nervous system
Chiroptera
Imunoistoquímica
Inflamação
Inflammation
Quirópteros
Rabies
Raiva
Sistema nervoso central
LA - pt
PY - 2009
SP - [275]-[275]
ST - O processo inflamatório, a resposta imune in situ e a morte neuronal em
sistema nervoso central de pacientes com raiva transmitida por morcegos
TI - O processo inflamatório, a resposta imune in situ e a morte neuronal em
sistema nervoso central de pacientes com raiva transmitida por morcegos
TT - Inflammatory process, in situ immune response and neuronal death in central
nervous system of patients with rabies transmitted by bats
UR - http://www.teses.usp.br/teses/disponiveis/5/5144/tde-09122009-171548/
publico/ElaineRanieroFernandesDoutorado.pdf
ID - 4950
ER -
TY - GEN
AB - O objetivo deste estudo foi avaliar a capacidade de produção de
nanopartículas de prata através do extrato da casca de romã, e produzir formulações
contendo estas nanopartículas para uso em feridas. Elas foram testadas quanto à
ação antimicrobiana, citotóxica e potencial cicatrizante. Para a produção das
nanopartículas de prata propôs-se uma síntese utilizando-se como base duas
metodologias já estabelecidas na literatura, utilizando-se para reação
carboximetilcelulose, propilenoglicol, nitrato de prata, água e extrato da casca de
romã como agente redutor. O extrato da casca de romã foi caracterizado em
parâmetros como pH, massa seca e quantidade de taninos bioativos (ácido elágico e
totais fenólicos expressos em ácido gálico). Os totais fenólicos do extrato foram
também dosados após seu aquecimento nas diferentes condições de tempo e temperatura
propostos para as sínteses das nanopartículas de prata (12 minutos, 1 hora e 2
horas, à 50ºC e 100ºC). As nanopartículas de prata produzidas foram, então,
adicionadas a uma solução contendo compostos para produção de formulações para
serem utilizadas no tratamento de feridas. Elas foram caracterizadas através de
espectroscopia UV-Visível, microscopia eletrônica de varredura (MEV), potencial
zeta e dosagem de íons remanescentes após as reações. A atividade antimicrobiana
tanto das nanopartículas como de suas formulações contra Candida albicans SC 5314 e
Staphylococcus aureus ATCC 25923 foi avaliada por meio do método da microdiluição.
Na avaliação dos extratos, apesar de ocorrer um aumento na concentração de totais
fenólicos com o aumento da temperatura, a concentração inibitória mínima (CIM)
manteve-se estável com valores de 391 µg/ml e 781 µg/ml para S. aureus e C.
albicans. A formação das nanopartículas de prata foi confirmada com a formação de
picos característicos na espectroscopia UV-Visível e pelas imagens de MEV, onde
verificou-se que a síntese com tempo de reação de 12 minutos e aquecimento a 50ºC
gerou nanopartículas mais uniformes e melhor distribuídas na formulação. As
sínteses propostas promoveram a redução iônica da prata de aproximadamente 100%,
independente do tempo temperatura utilizados na reação. Valores de CIM para as
nanopartículas de prata foram de 67,50 µg/ml e 68,75 µg/ml respectivamente para S.
aureus e C. albicans independente das variações das condições de síntese. Após a
seleção da síntese das nanopartículas de prata por 12 min à 50ºC, estas partículas
foram também caracterizadas por difração de raios-X e microscopia eletrônica de
transmissão (TEM), e as respectivas formulações por meio de espalhamento de luz
dinâmica, dosagem de íons livres, potencial zeta, MEV e TEM. Para essas formulações
foi também realizado um teste de estabilidade variando-se umidade e temperatura.
Além da atividade antimicrobiana contra Candida albicans SC 5314 e Staphylococcus
aureus ATCC 25923, a citotoxicidade em fibroblastos (L929) das nanopartículas de
prata e das formulações destas partículas e do extrato da casca de romã foram
também avaliadas. Para os extratos foram observados valores de 3,13, 86,39±0,96%
m/m, 3,64±0,03 mg/g, 392,0±9 mg/g respectivamente para pH, massa seca, ácido
elágico e totais fenólicos. Como controle, foram produzidas nanopartículas de prata
sintetizadas convencionalmente (AgNP química), e observou-se potencial redutor de
99,89% e 99,51% para as sínteses utilizando-se extrato de romã (AgNP green) e um
agente redutor químico convencional (AgNP química). Verificou-se a formação de
partículas com tamanhos médios de 89 e 19 nm para nanopartículas green e química. A
formulação contendo as nanopartículas de prata apresentaram um potencial
antimicrobiano expressivamente maior do que o princípio ativo isolado, sendo 255 e
4 vezes mais efetiva contra S. aureus e C. albicans, respectivamente. Os valores de
citotoxicidade foram consideravelmente menores para as nanopartículas de prata
sintetizadas pelo extr to de romã quando comparadas as produzidas
convencionalmente. De acordo com os valores de CIM e da citotoxicidade, a
concentração das formulações foi ajustada, gerando assim três formulações: i) AgNP
green, ii) AgNP química e iii) extrato de romã, nas concentrações de 337,5 µg/ml,
5,55 µg/ml e 94 µg/ml respectivamente. Para o estudo in vivo, utilizou-se como
controle um medicamento comercial contendo prata indicado para tratamento de
feridas (Sulfadiazina de prata). Foram selecionados noventa ratos Wistar machos com
peso médio de 180 gramas. Foi induzida diabetes nos ratos, e, em seguida, realizou-
se duas incisões no dorso dos animais e as lesões foram imediatamente infectadas
com S. aureus (ATCC 25923) e C. albicans (SC 5314). Após 24 h, os animais foram
divididos em grupos de acordo com as formulações propostas em cada tratamento,
seguindo-se um protocolo de duas vezes ao dia por 2, 7 e 14 dias. Após o período de
tratamento os animais foram eutanasiados e verificado o potencial reparador através
do índice de fechamento de ferida, avaliação do infiltrado inflamatório,
angiogenese, mieloperoxidase e hidroxiprolina. Ainda foi verificado o potencial
antimicrobiano das formulações através da contagem de células viáveis de cada
microrganismo infectado nas feridas. De forma geral, as formulações contendo
nanopartículas de prata mostraram os melhores resultados para o fechamento das
feridas, apresentando ainda uma atividade anti-inflamatória maior que a do extrato
de casca de romã, o qual apresentou atividade pró inflamatória. Todos os
tratamentos não foram capazes de reduzir de forma significativa o número de células
de C. albicans, enquanto para S. aureus todos os tratamentos apresentaram redução
significativa após quatorze dias de tratamento. Independente das nanopartículas de
prata serem produzidas quimicamente ou por meio do extrato da casca de romã, ambas
apresentaram considerável potencial de reparo de feridas infectadas em modelos in
vivo com ratos. Os achados das presentes pesquisas reforçam e estimulam o uso
potencial das nanopartículas de prata no tratamento de feridas, com destaque para a
síntese green por gerar menos danos ao meio ambiente e às pessoas envolvidas tanto
em sua produção como aos pacientes, e por apresentar, ainda, custo inferior quando
comparada às sínteses utilizando reagentes químicos e processos convencionais(AU)
The aim of this study was to investigate the production of silver nanoparticles
through peel extract of pomegranate, and produce formulations containing these
particles to be used in wound healings. Its antimicrobial action, cytotoxicity and
healing potential were tested. The synthesis of silver nanoparticles were based on
two methods proposed in the literature with some modifications, which were used
carboxymethylcellulose, propylene glycol, silver nitrate, water and peel extract of
pomegranate as reducing agent. The peel extract was characterized by pH, dry mass
and bioactive tannins (elagic acid and total phenols expressed as galic acid). The
total phenols were also quantified after being heated at 50ºC and 100ºC for 12
minutes, 1 hour and 2 hours. Then, silver nanoparticles were added in a solution
containing products to develop a formulation to be tested in wound healing. They
were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), zeta
potential and the quantification of remaining silver ions after the synthesis
reaction. The antimicrobial activity of the nanoparticles and formulations were
tested against Candida albicans (SC 5314) e Staphylococcus aureus (ATCC 25923) by
microdilution method. After submitting the peel extract to different conditions of
temperature and times (50ºC and 100ºC for 12 minutes, 1 hour and 2 hours), it was
noted that the values of the minimum inhibitory concentration was not affected and
were 391 µg/ml and 781 µg/ml for S. aureus and C. albicans. The formation of silver
nanoparticles was confirmed through the formation of characteristic peaks in the
UV-Vis spectroscopy and SEM images, and it was observed that the reaction at 50ºC
for 12 min produced silver nanoparticles with regular forms and better dispersed in
the formulation. The synthesis proposed promoted the reduction of silver ions at
about 100%, regardless of the time and temperature used in the reaction, which also
did not interfere in antimicrobial activity against C. albicans (68,75 µg/ml) and
S. aureus (67,50 µg/ml). After selecting the reaction at 50ºC for 12 min, the
silver nanoparticles produced ere also characterized by X-ray diffraction and
transmission electron microscopy (TEM), and the respective formulations through
dynamic light scattering, free ion dosage, zeta potential, SEM and TEM. The
stability test varying humidity and temperature was also performed for those
formulations. Besides antimicrobial assays, the cytotoxicity (L929 fibroblasts) of
the silver nanoparticles and the formulations of these particles and of the
pomegranate peel extract were evaluated. It was observed in the peel extract the
values of 3,13, 86,39±0,96% m/m, 3,64±0,03 mg/g and 392,0±9 mg/g respectively for
pH, dry mass, elagic acid and total phenols concentrations. Silver nanoparticles
produced by conventional chemical method was prepared and used as controls, and it
was noted the reduction potential of 99,89% and 99,51% for the synthesis using
pomegranate peel extract (AgNP green) and chemical reducing agent (AgNP chemical).
The averages sizes of green and chemical AgNP were 89 and 19 nm. The formulation
containing silver nanoparticles presented an antimicrobial potential expressively
higher than the active input, being 254 and 5- fold more effective against S.
aureus and C. albicans. Also, the cytotoxicity was notable reduced when silver
nanoparticles were produced using pomegranate peel extract. Based on the MIC values
and the cytotoxicity findings, the concentration of the formulations were
determined: i) AgNP green at 337.5 µg/ml, ii) AgNP chemical at 5.55 µg/ml, and iii)
pomegranate peel extract at 94 µg/ml. In the in vivo study, a commercial form of
silver (Sulfadizsine) to the wound healing was used as control. Ninety Wistar male
rats were selected, and, after inducing diabetes, two incisions on the dorsum of
the animals were made and followed infected with S. aureus (ATCC 25923) and C.
albicans (SC 5314). After the infection, the animals were treated with the
formulations twice a day for 2, 7 and 14 days. Then, the animals were euthanized
and the repair potential was verified through wound closure index, inflammatory
infiltrate evaluation, angiogenesis, myeloperoxidase and hydroxyproline. It was
also determined the antimicrobial potential by counting the viable cells of each
microorganism used to infect the wounds. In general, the formulations containing
silver nanoparticles promoted a better closure of the wounds, and a higher anti-
inflamatory activity than the peel extract formulation which otherwise presented a
pro-inflamatory effect. All formulations could not significantly reduce the viable
cells of C. albicans, while for S. aureus they reduced significantly the cells
after 14 days of treatment. Silver nanoparticles produced by both green and
conventional chemical process present notable potential in repairing infected
wounds in in vivo rat model. The findings of the present research strengthen and
stimulate the potential application of silver nanoparticles in wound healings,
highlighting the green production of these particles which apart from being lower
costly, it is ecofriendly and less detrimental to people involved in its production
and use(AU)
AU - Fernandes, Renan Aparecido
C1 - 20180828
C8 - biblio-911106
DA - 2017/00
DB - LILACS
KW - Biofilmes
Biofilms
Candida albicans
Nanotecnologia
Prata
Silver
Virulence nanotechnology
LA - en
PY - 2017
SP - 120-120
ST - Fitossíntese de nanopartículas de prata a partir de extrato de cascas de romã
e desenvolvimento de formulação para tratamento de feridas: avaliação
antimicrobiana, citotóxica e potencial cicatrizante em um modelo in vivo
TI - Fitossíntese de nanopartículas de prata a partir de extrato de cascas de romã
e desenvolvimento de formulação para tratamento de feridas: avaliação
antimicrobiana, citotóxica e potencial cicatrizante em um modelo in vivo
TT - Phytosynthesis of silver nanoparticles using pomegranate peel extract and
development of formulation for wound healing: antimicrobial and cytotoxicity
evaluation and repair potential in a in vivo model
UR - https://repositorio.unesp.br/handle/11449/151982
ID - 4929
ER -
TY - JOUR
AB - Objective: To evaluate the success and failure, apical sealing and
biocompatibility of silver amalgam, IRM®, SuperEBA® and MTA as retrograde filler
materials. Study design: A metaanalysis is made of filler materials in periapical
surgery, evaluating a total of 30 articles published in recent years. Results:
Percentage success with silver amalgam was 76.5% and slightly inferior to that
afforded by IRM®. Performance in turn increased considerably when the materials
used were SuperEBA® or MTA. As regards marginal leakage, MTA with a mean leakage
time of 65.5 days afforded the best results, followed by SuperEBA®, IRM® and silver
amalgam. MTA was the most biocompatible of the materials studied, with practically
no inflammatory response, while inflammation proved mild or moderate with
SuperEBA®, mild with IRM®, and moderate to severe in the case of silver amalgam.
Tissue regeneration was only observed with MTA, in the same way as cement
appositioning. Bone neoformation was observed with all four filler materials.
Conclusions: MTA appears to be an ideal material, though the results obtained
require confirmation by in vivo studies. © Medicina Oral S. L. C.I.F.
AU - Fernandez Yanez Sanchez, Á
AU - Leco Berrocal, M. I.
AU - Martínez González, J. M.
DB - Scopus
IS - 3
KW - Apical sealing
Apicoectomy
Irm®
MTA
Periapical surgery
Retrograde filler materials
Silver amalgam
SuperEBA®
Aluminum Compounds
Calcium Compounds
Dental Amalgam
Dentin-Bonding Agents
Drug Combinations
Humans
Methylmethacrylates
Oxides
Periapical Diseases
Silicates
amalgam
biomaterial
filler
silver
article
biocompatibility
clinical trial
cytotoxicity
dental surgery
disease severity
human
inflammation
meta analysis
outcome assessment
periapical surgery
surgical technique
systematic review
tooth filling
tooth root
treatment outcome
M3 - Article
PY - 2008
SP - 180-185
ST - Metaanalysis of filler materials in periapical surgery
T2 - Medicina Oral, Patologia Oral y Cirugia Bucal
TI - Metaanalysis of filler materials in periapical surgery
41849150775&partnerID=40&md5=aaacde848b86cd37e54c54c6b5969740
VL - 13
ID - 5761
ER -
TY - JOUR
AB - We characterized a method to conjugate functional silver nanoparticles with
vasoactive intestinal peptide (VIP), which could be used as a working model for
further tailor-made applications based on VIP surface functionality. Despite
sustained interest in the therapeutic applications of VIP, and the fact that its
drugability could be largely improved by the attachament to functionalized metal
nanoparticles, no methods have been described so far to obtain them. Materials &
methods: VIP was conjugated to tiopronin-capped silver nanoparticles of a narrow
size distribution, by means of proper linkers, to obtain VIP functionalized silver
nanoparticles with two different VIP orientations (Ag-tiopronin-PEG-succinic-
[His]VIP and Ag-tiopronin-PEG-VIP[His]). VIP intermediate nanoparticles were
characterized by transmission-electron microscopy and Fourier transform infrared
spectroscopy. VIP functionalized silver nanoparticles cytotoxicity was determined
by lactate dehydrogenase release from mixed glial cultures prepared from cerebral
cortices of 1-3 days-old C57/Bl mice. Cells were used for lipopolysaccharide
stimulation at day 18-22 of culture. Results: Two different types of VIP-
functionalized silver nanoparticles were obtained; both expose the C-terminal part
of the neuropeptide, but in the first type VIP is attached to silver nanoparticle
through its free amine terminus (Ag-tiopronin-PEG-succinic-[His]VIP), while in the
second type, VIP N-terminus remains free (Ag-tiopronin-PEG-VIP[His]). VIP-
functionalized silver nanoparticles did not compromise cellular viability and
inhibited microglia-induced stimulation under inflammatory conditions. Conclusion:
The chemical synthesis procedure developed to obtain VIP-functionalized silver
nanoparticles rendered functional products, in terms of biological activity. The
two alternative orientations designed, reduced the constraints for chemical
synthesis that depends on the nanosurface to be functionalized. Our study provides,
for the first time, a proof of principle to enhance the therapeutic potential of
VIP with the valuable properties of metal nanoparticles for imaging, targeting and
drug delivery. © 2009 Future Medicine Ltd.
AU - Klippstein, R.
AU - Gonzalez-Rey, E.
AU - Mejias, J. A.
AU - Pozo, D.
DB - Scopus
DO - 10.2217/nnm.09.79
IS - 8
KW - Bioimaging
Immune system
Inflammation
Microglia
Neurodegeneration
Neuropeptide
Silver nanoparticle
Theranostic agent
Animals
Cell Survival
Cells, Cultured
Interleukin-10
Interleukin-6
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Molecular Structure
Nanotechnology
Silver
Vasoactive Intestinal Peptide
CD68 antigen
histidinylserylaspartylalanylleucylphenylalanylthreonylaspartylthreonyltyrosylthreo
nylarginylleucylarginyllysylglutaminylmethionylalanylmethionyllysyllysyltyrosylleuc
ylasparaginylserylvalylleucylasparagine
interleukin 10
interleukin 6
nanoparticle
silver
tiopronin
unclassified drug
vasoactive intestinal polypeptide
article
carboxy terminal sequence
cytotoxicity
drug structure
drug synthesis
glia cell
hydrophilicity
immunocytochemistry
nonhuman
particle size
priority journal
M3 - Article
PY - 2009
SP - 919-930
ST - Chemical synthesis and characterization of silver-protected vasoactive
intestinal peptide nanoparticles
T2 - Nanomedicine
TI - Chemical synthesis and characterization of silver-protected vasoactive
intestinal peptide nanoparticles
73349118484&doi=10.2217%2fnnm.09.79&partnerID=40&md5=1398dc1dec8bee3d4a43980eb8a693
ae
VL - 4
ID - 5706
ER -
TY - JOUR
AB - Spondyloarthropathies are a group of chronic inflammatory disorders that
involve the joints of the axial skeleton, peripheral joints and have extra-
articular manifestations. Treatment includes inhibitors of tumor necrosis factor α.
Currently there are five approved inhibitors: a soluble receptor, Etanercept and
four monoclonal. Etanercept has very low toxicity with pulmonary adverse reactions
being very rare. We present the case of a patient who developed respiratory
symptoms and pulmonary infiltrates of rapid evolution after the third dose of
treatment with Etanercept. © 2020 The Authors
AU - Fernández-Trujillo, L.
AU - Iriarte, M. B.
AU - Puerta, G.
AU - Morales, E. I.
AU - Sua, L. F.
AU - Cañas, C. A.
C7 - 101079
DB - Scopus
DO - 10.1016/j.rmcr.2020.101079
KW - Anti-TNF
Case report
Etanercept
Pulmonary granulomatosis
Spondyloarthropathy
TNF-α
bilirubin
creatinine
etanercept
etoricoxib
hemoglobin
methylprednisolone
prednisone
vitamin D
adult
arthralgia
Article
bone marrow edema
bronchoscopy
case report
clinical article
coughing
crackle
cytology
disease association
dry cough
dyspnea
electrocardiogram
female
granulomatous pneumonitis
human
human cell
human tissue
laboratory test
lung lavage
myalgia
nuclear magnetic resonance imaging
pain
pneumonia
priority journal
provocation test
sacroiliitis
silver staining
spondyloarthropathy
transbronchial biopsy
transthoracic echocardiography
M3 - Article
PY - 2020
ST - Early instauration granulomatous pneumonitis associated with use of
etanercept in seronegative spondyloarthropathy: Case report
T2 - Respiratory Medicine Case Reports
TI - Early instauration granulomatous pneumonitis associated with use of
etanercept in seronegative spondyloarthropathy: Case report
85084975572&doi=10.1016%2fj.rmcr.2020.101079&partnerID=40&md5=eb63ce9a113008d0ff553
2bac226a3bd
VL - 30
ID - 5379
ER -
TY - JOUR
AB - Bacterial, protozoan and other microbial infections share an accelerated
metabolic rate. In order to ensure a proper functioning of cell replication and
proteins and nucleic acids synthesis processes, folate metabolism rate is also
increased in these cases. For this reason, folic acid antagonists have been used
since their discovery to treat different kinds of microbial infections, taking
advantage of this metabolic difference when compared with human cells. However,
resistances to these compounds have emerged since then and only combined therapies
are currently used in clinic. In addition, some of these compounds have been found
to have an immunomodulatory behavior that allows clinicians using them as anti-
inflammatory or immunosuppressive drugs. Therefore, the aim of this review is to
provide an updated state-of-the-art on the use of antifolates as antibacterial and
immunomodulating agents in the clinical setting, as well as to present their action
mechanisms and currently investigated biomedical applications. © 2019 by the
authors. Licensee MDPI, Basel, Switzerland.
AU - Fernández-Villa, D.
AU - Aguilar, M. R.
AU - Rojo, L.
C7 - 4996
DB - Scopus
DO - 10.3390/ijms20204996
IS - 20
KW - Antibacterials
Antibiotics
Antifolates
Antimalarial
Folic acid antagonists
Immunomodulation
Sulfonamides
Animals
Drug Resistance
Folic Acid Antagonists
Humans
Immunologic Factors
carbonate dehydratase
cotrimoxazole
dihydrofolate reductase
dihydropteroate synthase
endothelial nitric oxide synthase
endothelin 1
erythromycin
folic acid
folic acid antagonist
G protein coupled receptor
glutamate synthase
interleukin 17
interleukin 8
mafenide acetate
n4 acetylsulfafurazole
sulfadiazine
sulfadiazine silver
sulfafurazole
sulfathiazole
thymidylate synthase
vancomycin
antiinfective agent
immunologic factor
apoptosis
cancer growth
CD4+ T lymphocyte
CD8+ T lymphocyte
nonhuman
prostatitis
pruritus
psoriasis
Review
toxoplasmosis
ulcerative colitis
wound healing
animal
chemistry
drug effect
drug resistance
human
immunomodulation
M3 - Review
PY - 2019
ST - Folic acid antagonists: Antimicrobial and immunomodulating mechanisms and
applications
TI - Folic acid antagonists: Antimicrobial and immunomodulating mechanisms and
applications
85073095400&doi=10.3390%2fijms20204996&partnerID=40&md5=97b37543682d9443d70846dfd8e
6e490
VL - 20
ID - 5404
ER -
TY - GEN
AB - Este estudo teve como objetivo avaliar a biocompatibilidade, através de
análise histopatológica e de imuno-histoquímica, de um novo cimento reparador à
base de MTA com alta plasticidade: MTA HP (Angelus Londrina, PR). O MTA branco
(Angelus Londrina, PR), e um material a base de óxido de zinco e eugenol (IRM,
Dentsply, Petrópolis, RJ) foram utilizados como referências para comparação. Para
isso, trinta ratos machos de linhagem Wistar tiveram inoculados no tecido
subcutâneo um tubo de polietileno vazio (controle negativo) e mais três tubos, cada
um preenchido com um dos materiais testados. Os animais foram eutanasiados após 7,
30 e 60 dias da implantação dos tubos e as amostras foram fixadas e incluídas em
parafina. Os cortes histológicos foram corados com hematoxilina e eosina e
tricômico de gomori para avaliação das reações inflamatórias e a presença de
angiogênese foi realizada utilizando o marcador VEGF (do inglês vascular
endothelial growth factor). Os cortes também foram corados com Picrosirius Red para
quantificar as fibras colágenas do tipo I e tipo III, assim como a coloração de
Weigert foi realizada para observar as fibras elásticas. Os dados não-paramétricos
foram analisados usando o ensaio de Kruskal-Wallis seguido do teste de Dunn. Os
níveis de significância adotados foram de 5% (P < 0,05). Os resultados mostraram
diferença significativa da resposta inflamatória após 60 dias entre os grupos IRM e
tubo vazio (P < 0,05). O MTA HP apresentou biocompatibilidade similar ao MTA branco
e ao grupo controle negativo em todos os períodos experimentais. Além disso, após 7
dias o MTA HP estimulou a angiogênese de forma menos acentuada que o MTA branco,
assim como apresentou inicialmente um remodelamento mais lento da matriz
extracelular quando comparado ao MTA branco e o IRM. Foi observado uma diminuição
da espessura da cápsula fibrosa, da quantidade de fibras elásticas e da
imonumarcação com VEGF em todos os grupos experimentais e controle negativo ao
longo do processo de cicatrização. Após 60 dias os grupos experimentais
apresentaram matriz extracelular com tecido conjuntivo mais maduro, com
predominância de fibras colágenas do tipo I. De acordo com os resultados obtidos no
presente estudo, pode-se concluir que o novo cimento reparador com alta
plasticidade, MTA HP, apresentou-se biocompatível em todos os períodos
experimentais, com resultados similares aos grupos controle negativo e
experimentais com MTA branco e IRM.
This study evaluated the biocompatibility, through histopathological analysis and
immunohistochemistry of a new repair cement based on MTA with high plasticity: MTA
HP (Angelus Londrina, PR). White MTA (Angelus Londrina, PR), and a material based
on zinc oxide and eugenol (IRM, Dentsply, Petrópolis, RJ) were used as references
for comparison. Thirty male Wistar rats had inoculated into the subcutaneous tissue
an empty polyethylene tube (negative control) and three more tubes, each filled
with one of the tested materials. The animals were euthanized after 7, 30 and 60
days of tube implantation and the specimens were fixed and embedded in paraffin.
The sections were stained with hematoxylin and eosin and gomori trichrome to assess
inflammatory reactions, and the presence of angiogenesis was performed using the
VEGF (vascular endothelial growth factor) marker. The sections were also stained
with Picrosirius Red to quantify as type I and type III collagen fibers, as well as
a Weigert staining was performed to observe elastic fibers. Non-parametric data
were analyzed using the Kruskal-Wallis assay followed Dunn's test. The significance
levels adopted were 5% (P < 0.05). The results demonstrated a significant
difference in inflammatory response after 60 days between IRM and empty tube groups
(P < 0.05). MTA HP showed similar biocompatibility to the White MTA and the
negative control group in all experimental periods. Furthermore, after 7 days MTA
HP stimulated less pronounced angiogenesis than White MTA, as it initially
exhibited slower extracellular matrix remodeling when compared to White MTA and
IRM. A decrease in the thickness of the fibrous capsule, the amount of elastic
fibers and the immunostaining with VEGF in all experimental groups and control
throughout the healing process was observed. After 60 days, the experimental groups
presented extracellular matrix with more mature connective tissue, with
predominance of type I collagen fibers. According to the results obtained in the
present study, it can be concluded that the new repair cement with high plasticity,
MTA HP, was biocompatible in all the experimental periods, presenting similar
results to the control and experimental groups with White MTA and IRM.
AU - Ferreira, Cláudio Malizia Alves
C1 - 20190918
C8 - biblio-1016644
DA - 2018/00
DB - LILACS
KW - Agregado trióxido mineral
Biocompatibilidade
Biocompatibility
Imuno-histoquímica
Mineral trioxide aggregate
Subcutaneous tissue of rats
Tecido subcutâneo de ratos
LA - pt
PY - 2018
SP - 76-76
ST - Reação tecidual em subcutâneo de ratos frente a um cimento reparador à base
de MTA com alta plasticidade
TI - Reação tecidual em subcutâneo de ratos frente a um cimento reparador à base
de MTA com alta plasticidade
TT - Tissue reaction to repair cement based on MTA with high plasticity in
subcutaneous of rats
UR - http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=16327
ID - 4925
ER -
TY - JOUR
AB - The use of natural resources for the prevention and treatment of diseases
considered fatal to humanity has evolved. Several medicinal plants have nutritional
and pharmacological potential in the prevention and treatment of viral infections,
among them, turmeric, which is recognized for its biological properties associated
with curcuminoids, mainly represented by curcumin, and found mostly in rhizomes.
The purpose of this review was to compile the pharmacological activities of
curcumin and its analogs, aiming at stimulating their use as a therapeutic strategy
to treat infections caused by RNA genome viruses. We revisited its historical
application as an anti-inflammatory, antioxidant, and antiviral agent that combined
with low toxicity, motivated research against viruses affecting the population for
decades. Most findings concentrate particularly on arboviruses, HIV, and the recent
SARS-CoV-2. As one of the main conclusions, associating curcuminoids with
nanomaterials increases solubility, bioavailability, and antiviral effects,
characterized by blocking the entry of the virus into the cell or by inhibiting key
enzymes in viral replication and transcription.
AN - WOS:000774677500001
AU - Ferreira, L. L. C.
AU - Abreu, M. P.
AU - Costa, C. B.
AU - Leda, P. O.
AU - Behrens, M. D.
AU - dos Santos, E. P.
C6 - MAR 2022
DA - JUN
DO - 10.1007/s12560-022-09514-3
IS - 2
PY - 2022
SN - 1867-0334
1867-0342
SP - 120-137
ST - Curcumin and Its Analogs as a Therapeutic Strategy in Infections Caused by
RNA Genome Viruses
T2 - FOOD AND ENVIRONMENTAL VIROLOGY
TI - Curcumin and Its Analogs as a Therapeutic Strategy in Infections Caused by
RNA Genome Viruses
VL - 14
ID - 6611
ER -
TY - JOUR
AB - In previous work, we developed novel antibacterial hybrid coatings based on
dextran containing dispersed Ag NPs (similar to 5 nm, DEX-Ag) aimed to offer dual
protection against two of the most common complications associated with implant
surgery, infections and rejection of the implant. However, their blood-material
interactions are unknown. In this study, we assess the hemocompatibility and
biocompatibility of DEX-Ag using fresh blood and two cell lines of the immune
system, monocytes (THP-1 cells) and macrophages (PMA-stimulated THP-1 cells).
Glass, polyurethane (PU) and bare dextran (DEX) were used as reference surfaces.
PU, DEX and DEX-Ag exhibited non-hemolytic properties. Relative to glass (100%),
platelet attachment on PU, DEX and DEX-Ag was 15%, 10% and 34%, respectively.
Further, we assessed cell morphology and viability, pro-inflammatory cytokines
expression (TNF-alpha and IL-1 beta), pro-inflammatory eicosanoid expression
(Prostaglandin E-2, PGE(2)) and release of reactive oxygen species (ROS, superoxide
and H2O2) following incubation of the cells with the surfaces. The morphology and
cell viability of THP-1 cells were not affected by DEX-Ag whereas DEX-Ag minimized
spreading of PMA-stimulated THP-1 cells and caused a reduction in cell viability
(16% relative to other surfaces). Although DEX-Ag slightly enhanced release of ROS,
the expression of pro-inflammatory cytokines remained minimal with similar levels
of PGE(2), as compared to the other surfaces studied. These results highlight low
toxicity of DEX-Ag and hold promise for future applications in vivo. (C) 2013
Elsevier Inc. All rights reserved.
AN - WOS:000326141400016
AU - Ferrer, M. C. C.
AU - Eckmann, U. N.
AU - Composto, R. J.
AU - Eckmann, D. M.
DA - NOV 1
DO - 10.1016/j.taap.2013.07.023
IS - 3
PY - 2013
SN - 0041-008X
1096-0333
SP - 703-712
ST - Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid
films
TI - Hemocompatibility and biocompatibility of antibacterial biomimetic hybrid
films
VL - 272
ID - 6224
ER -
TY - JOUR
AB - Aim The aim of this study was to evaluate pigmentation and tissue response to
four endodontic sealers placed in the oral mucosa of rabbits by either submucous
injection or implant in polyethylene tubes. Methodology Thirty white New Zealand
rabbits were divided randomly into two groups of eight for N-Rickert and AH-26, and
two groups of seven for Fillcanal and Sealer 26. On the right side of the filter,
corresponding to the gingivo-labial sulcus in humans, the sealer was injected; on
the left side the sealer was placed within a polyethylene tube and implanted.
Direct clinical observations were made at 30, 60 and 90 days. The animals were then
sacrificed for histological analysis. Results After 60 days of observation N-
Rickert and AH-26 produced tattoos that became larger by 90 days. Submucous
injection produced larger and more numerous pigmentation. when compared to implant
in polyethylene tubes. N-Rickert sealer displayed larger and more numerous tattoos
when compared to AH-26, Histological analysis showed no differences between the two
methods of implantation. All sealers elicited some kind of inflammatory response:
the most irritant was Fillcanal, followed by N-Rickert and AH-26, Sealer 26
elicited a mild reaction only. Conclusions Under the conditions of this study there
was no relationship between the method of implantation and the tissue response: the
silver-containing sealers produced pigmentation. and the concentration of silver
influenced the quantity and size of the tattoos. The sealers elicited various
responses when in direct contact with the surrounding tissues; the calcium
hydroxide-containing sealer had enhanced healing when compared to the other
sealers.
AN - WOS:000169890000006
AU - Figueiredo, J. A. P.
AU - Pesce, H. F.
AU - Gioso, M. A.
AU - Figueiredo, M. A. Z.
DA - JUL
DO - 10.1046/j.1365-2591.2001.00407.x
IS - 5
PY - 2001
SN - 0143-2885
SP - 377-385
ST - The histological effects of four endodontic sealers implanted in the oral
mucose: submucous injection versus implant in polyethylene tubes
T2 - INTERNATIONAL ENDODONTIC JOURNAL
TI - The histological effects of four endodontic sealers implanted in the oral
mucose: submucous injection versus implant in polyethylene tubes
VL - 34
ID - 6722
ER -
TY - JOUR
AB - The study aims to evaluate the impact of silver nanoparticles,
phytosynthesized with polyphenols from Sambucus nigra L. (SN) fruit extract (AgSN),
on dysplastic oral keratinocytes (DOK) and human gingival fibroblasts (HGF) in
terms of cell viability and apoptosis. The morphology and ultrastructure of treated
cells as well as the mechanisms involved in cell death induction were investigated
in DOK cultures. The structure of AgSN was studied by using the appropriate
analysis tools such as UV–Vis, transmission electron microscopy, Raman
spectroscopy, dynamic light scattering (DLS) and zeta potential assessment. DOK and
HGF were treated either with silver nanoparticles capped with Sambucus nigra L.
extract or with SN extract. Untreated cells were used as controls. Viability was
determined by MTS assay. Transmission electronic microscopy (TEM) was used to
evaluate the intracellular localization of the nanoparticles at 4 and 24 h. Annexin
V-FITC/propidium iodide staining and the expressions of p53, BAX, BCL2, NFkB,
phosphorylated NFkB (pNFkB), pan AKT, pan phosphoAKT, LC3B and ɣH2AX were evaluated
to quantify the cell death. ELISA measurements of TNF-α and TRAIL was used for the
study of the inflammatory response. Oxidative stress damage induced by
nanoparticles was assessed by the malondialdehyde (MDA) level. Silver nanoparticles
stimulated HGF proliferation and significantly diminished DOK viability at doses
higher than 20 μg/ml. TEM analysis demonstrated the internalization of silver
nanoparticles and showed ultrastructural changes of cells such as the appearance of
vacuoles, autophagosomes, endosomes. AgSN inhibited the pro-survival molecules and
regulators of apoptosis, diminished oxidative stress and inflammation and induced
cell death through various mechanisms: necrosis, autophagy and DNA lesions. SN
extract had antioxidant and anti-inflammatory effect and increased the DNA lesions
and autophagy in DOK cells. Silver nanoparticles protected the normal cells and
induced cell death in dysplastic cells by different mechanisms thus offering
beneficial effects in the treatment of oral dysplasia. © 2021 Elsevier B.V.
AU - Filip, G. A.
AU - Florea, A.
AU - Olteanu, D.
AU - Clichici, S.
AU - David, L.
AU - Moldovan, B.
AU - Cenariu, M.
AU - Scrobota, I.
AU - Potara, M.
AU - Baldea, I.
C7 - 111974
DB - Scopus
DO - 10.1016/j.msec.2021.111974
KW - Autophagy
Inflammation
Necrosis
Oral dysplastic keratinocytes
Oxidative stress
Fruit
Humans
Metal Nanoparticles
Plant Extracts
Sambucus nigra
Silver
Biochemistry
Cell culture
Cell death
Damage detection
Fruits
Metal nanoparticles
Silver compounds
Transmissions
metal nanoparticle
plant extract
silver
Beneficial effects
Different mechanisms
Human gingival fibroblasts
Intracellular localization
Transmission electronic microscopies
Ultrastructural changes
fruit
human
M3 - Article
PY - 2021
ST - Biosynthesis of silver nanoparticles using Sambucus nigra L. fruit extract
for targeting cell death in oral dysplastic cells
TI - Biosynthesis of silver nanoparticles using Sambucus nigra L. fruit extract
for targeting cell death in oral dysplastic cells
85101424473&doi=10.1016%2fj.msec.2021.111974&partnerID=40&md5=d13233c74f076e4f50d9e
685f622c542
VL - 123
ID - 5252
ER -
TY - JOUR
AB - The purpose of our study is to investigate the comparative effects of
materials based on silver and gold nanoparticles functionalized with polyphenols
from Cornus Mas extract (AgNPs-CM and AuNPs-CM) in vivo on experimental
inflammation. The nanoparticles were obtained at room temperature under UV
irradiation and were characterized by different methods: ultraviolet-visible
spectroscopy, transmission electron microscopy, X ray diffraction, Fourier
transform infrared spectroscopy and dynamic light scattering. The modulatory
effects of AgNPs-CM and AuNPs-CM on inflammation were quantified by oxidative
stress parameters, pro and anti-inflammatory cytokines levels and apoptosis
assessment at 2 h, 24 and 48 h after induction of inflammation with carrageenan in
the paw tissue of Wistar rats. Our results showed that silver and gold
nanoparticles only partial and for a short period have mobilized the antioxidant
defense mechanisms. In addition, they diminished inflammation and apoptosis in the
early stage while later, at 48 h, exerted an immunomodulatory effect, activated ERK
½ and induced apoptosis. The photoreduced silver and gold nanoparticles,
functionalized with natural compounds, modulated the inflammation in a similar
manner in the soft tissue injected with carrageenan. In order to decipher the
mechanisms involved in interactions of metallic nanoparticles with biological
systems and for a complete assessment of the risks and benefits of these products
in clinical practice long term studies are necessary. © 2018 Elsevier B.V.
AU - Filip, G. A.
AU - Moldovan, B.
AU - Baldea, I.
AU - Olteanu, D.
AU - Suharoschi, R.
AU - Decea, N.
AU - Cismaru, C. M.
AU - Gal, E.
AU - Cenariu, M.
AU - Clichici, S.
AU - David, L.
DB - Scopus
DO - 10.1016/j.jphotobiol.2018.12.006
KW - Apoptosis
Inflammation
Polyphenols
Rats
Animals
Cornus
Gold
Metal Nanoparticles
Plant Extracts
Rats, Wistar
Silver
Ultraviolet Rays
Cornus Mas extract
cytokine
gold nanoparticle
plant extract
polyphenol
silver nanoparticle
unclassified drug
gold
metal nanoparticle
silver
animal experiment
animal model
animal tissue
apoptosis
Article
carrageenan-induced inflammation
cherry
comparative effectiveness
controlled study
Cornus Mas
drug effect
drug efficacy
drug mechanism
drug response
drug synthesis
immunomodulation
in vivo study
nonhuman
oxidative stress
priority journal
rat
room temperature
treatment outcome
ultraviolet irradiation
Wistar rat
X ray diffraction
animal
chemistry
green chemistry
inflammation
pathology
M3 - Article
PY - 2019
SP - 26-37
ST - UV-light mediated green synthesis of silver and gold nanoparticles using
Cornelian cherry fruit extract and their comparative effects in experimental
inflammation
T2 - Journal of Photochemistry and Photobiology B: Biology
TI - UV-light mediated green synthesis of silver and gold nanoparticles using
Cornelian cherry fruit extract and their comparative effects in experimental
inflammation
85058369716&doi=10.1016%2fj.jphotobiol.2018.12.006&partnerID=40&md5=42f8a58e9db584c
7c5d722f5de711b8e
VL - 191
ID - 5450
ER -
TY - JOUR
AB - Engineered T cell therapies such as chimeric antigen receptor (CAR)
expressing T cells (CAR-T cells) have great potential to treat many human diseases;
however, inflammatory toxicities associated with these therapies present safety
risks and can greatly limit its widespread use. This article briefly reviews our
current understanding of mechanisms for inflammatory toxicities during CAR T-cell
therapy, current strategies for management and mitigation of these risks and
highlights key areas of knowledge gap for future research.
AN - WOS:000668926400001
AU - Fischer, J. W.
AU - Bhattarai, N.
C7 - 693016
DA - JUN 18
DO - 10.3389/fimmu.2021.693016
PY - 2021
SN - 1664-3224
ST - CAR-T Cell Therapy: Mechanism, Management, and Mitigation of Inflammatory
Toxicities
T2 - FRONTIERS IN IMMUNOLOGY
TI - CAR-T Cell Therapy: Mechanism, Management, and Mitigation of Inflammatory
Toxicities
VL - 12
ID - 6131
ER -
TY - JOUR
AB - Vaccination with live attenuated Leishmania parasites such as centrin deleted
Leishmania donovani (LdCen(-/-)) against visceral leishmaniasis has been reported
extensively. The protection induced by LdCen(-/-) parasites was mediated by both
CD4(+) and CD8(+) T cells. While the host immune mediators of protection are known,
parasite determinants that affect the CD4(+) and CD8(+) T cell populations remain
unknown. Parasite encoded inflammatory cytokine MIF has been shown to modulate the
T cell differentiation characteristics by altering the inflammation induced
apoptosis during contraction phase in experimental infections with Leishmania or
Plasmodium. Neutralization of parasite encoded MIF either by antibodies or gene
deletion conferred protection in Plasmodium and Leishmania studies. We investigated
if the immunogenicity and protection induced by LdCen(-/-) parasites is affected by
deleting MIF genes from this vaccine strain. Our results showed that
LdCen(-/-)MIF(-/-) immunized group presented higher percentage of CD4(+) and CD8(+)
central memory T cells, increased CD8(+) T cell proliferation after challenge
compared to LdCen(-/-) immunization. LdCen(-/-)MIF(-/-) immunized group presented
elevated production of IFN-& gamma;(+) and TNF-& alpha;(+) CD4(+) T cells
concomitant with a reduced parasite load in spleen and liver compared to
LdCen(-/-)group following challenge with L. infantum. Our results demonstrate the
role of parasite induced factors involved in protection and long-term immunity of
vaccines against VL.
AN - WOS:001022550400001
AU - Fiuza, J. A.
AU - Gannavaram, S.
AU - Gaze, S. T.
AU - de Ornellas, L. G.
AU - Alves, E. A.
AU - Ismail, N.
AU - Nakhasi, H. L.
AU - Correa-Oliveira, R.
C7 - 7362
DA - MAY 5
DO - 10.1038/s41598-023-34333-2
IS - 1
PY - 2023
SN - 2045-2322
ST - Deletion of MIF gene from live attenuated LdCen(-/-) parasites enhances
protective CD4(+) T cell immunity
TI - Deletion of MIF gene from live attenuated LdCen(-/-) parasites enhances
protective CD4(+) T cell immunity
VL - 13
ID - 6670
ER -
TY - JOUR
AB - Background: The present study aimed to evaluate the potential differences in
the biological effects of two types of spherical silver particles of 20 and 200 nm
(Ag20 and Ag200), and of PVP-coated silver nanowires (AgNWs) with a diameter of 50
nm and length up to 50 μm, using a complex 3D model representative for the alveolar
barrier cultured at air-liquid interface (ALI). The alveolar model was exposed to
0.05, 0.5 and 5 μg/cm 2 of test compounds at ALI using a state-of-the-art exposure
system (Vitrocell™Cloud System). Endpoints related to the oxidative stress
induction, anti-oxidant defence mechanisms, pro-inflammatory responses and cellular
death were selected to evaluate the biocompatibility of silver particles and
nanowires (AgNMs) and to further ascribe particular biological effects to the
different morphologic properties between the three types of AgNMs evaluated.
Results: Significant cytotoxic effect was observed for all three types of AgNMs at
the highest tested doses. The increased mRNA levels of the pro-apoptotic gene CASP7
suggests that apoptosis may occur after exposure to AgNWs. All three types of AgNMs
increased the mRNA level of the anti-oxidant enzyme HMOX-1 and of the metal-binding
anti-oxidant metallothioneins (MTs), with AgNWs being the most potent inducer. Even
though all types of AgNMs induced the nuclear translocation of NF-kB, only AgNWs
increased the mRNA level of pro-inflammatory mediators. The pro-inflammatory
response elicited by AgNWs was further confirmed by the increased secretion of the
10 evaluated interleukins. Conclusion: In the current study, we demonstrated that
the direct exposure of a complex tetra-culture alveolar model to different types of
AgNMs at ALI induces shape- and size-specific biological responses. From the three
AgNMs tested, AgNWs were the most potent in inducing biological alterations.
Starting from 50 ng/cm 2 , a dose representative for an acute exposure in a high
exposure occupational setting, AgNWs induced prominent changes indicative for a
pro-inflammatory response. Even though the acute responses towards a dose
representative for a full-lifetime exposure were also evaluated, chronic exposure
scenarios at low dose are still unquestionably needed to reveal the human health
impact of AgNMs during realistic conditions. © 2019 The Author(s).
AU - Fizeşan, I.
AU - Cambier, S.
AU - Moschini, E.
AU - Chary, A.
AU - Nelissen, I.
AU - Ziebel, J.
AU - Audinot, J. N.
AU - Wirtz, T.
AU - Kruszewski, M.
AU - Pop, A.
AU - Kiss, B.
AU - Serchi, T.
AU - Loghin, F.
AU - Gutleb, A. C.
C7 - 14
DB - Scopus
DO - 10.1186/s12989-019-0297-1
IS - 1
KW - Air-liquid interface
Inflammation
Silver nanowires
Tetra-culture
Air Pollutants
Blood-Air Barrier
Cell Survival
Cells, Cultured
Cytokines
Endothelial Cells
Gene Expression
Humans
Metal Nanoparticles
Models, Biological
Nanowires
Oxidative Stress
Particle Size
Pulmonary Alveoli
Silver
caspase 7
cytokine
endothelial leukocyte adhesion molecule 1
heme oxygenase 1
intercellular adhesion molecule 1
interleukin 6
interleukin 8
messenger RNA
metallothionein
nanowire
silver nanoparticle
silver nanowire
transcription factor
unclassified drug
metal nanoparticle
silver
air liquid interface cell culture
alveolar barrier culture
apical compartment
apoptosis
art
Article
biocompatibility
CASP7 gene
cell culture
cell transfer
controlled study
cytokine release
cytotoxicity
death
defense mechanism
dose response
E SELECTIN gene
gene
gene expression
human
human cell
ICAM1 gene
IL 6 gene
IL 8 gene
IL6 gene
IL8 gene
in vitro study
inflammation
long term exposure
metabolic disorder
morphology
oxidative stress
particle size
priority journal
stress
VCAM1 gene
air pollutant
biological model
cell survival
coculture
drug effect
endothelium cell
genetics
immunology
lung alveolus
lung gas exchange
metabolism
M3 - Article
PY - 2019
ST - In vitro exposure of a 3D-tetraculture representative for the alveolar
barrier at the air-liquid interface to silver particles and nanowires
TI - In vitro exposure of a 3D-tetraculture representative for the alveolar
85063801688&doi=10.1186%2fs12989-019-0297-
1&partnerID=40&md5=6a33bf587473bc58b6cff805634adb11
VL - 16
ID - 5376
ER -
TY - JOUR
AB - BackgroundThe present study aimed to evaluate the potential differences in
the biological effects of two types of spherical silver particles of 20 and 200nm
(Ag20 and Ag200), and of PVP-coated silver nanowires (AgNWs) with a diameter of
50nm and length up to 50m, using a complex 3D model representative for the alveolar
barrier cultured at air-liquid interface (ALI). The alveolar model was exposed to
0.05, 0.5 and 5g/cm(2) of test compounds at ALI using a state-of-the-art exposure
system (VitrocellCloud System). Endpoints related to the oxidative stress
induction, anti-oxidant defence mechanisms, pro-inflammatory responses and cellular
death were selected to evaluate the biocompatibility of silver particles and
nanowires (AgNMs) and to further ascribe particular biological effects to the
different morphologic properties between the three types of AgNMs
evaluated.ResultsSignificant cytotoxic effect was observed for all three types of
AgNMs at the highest tested doses. The increased mRNA levels of the pro-apoptotic
gene CASP7 suggests that apoptosis may occur after exposure to AgNWs. All three
types of AgNMs increased the mRNA level of the anti-oxidant enzyme HMOX-1 and of
the metal-binding anti-oxidant metallothioneins (MTs), with AgNWs being the most
potent inducer. Even though all types of AgNMs induced the nuclear translocation of
NF-kB, only AgNWs increased the mRNA level of pro-inflammatory mediators. The pro-
inflammatory response elicited by AgNWs was further confirmed by the increased
secretion of the 10 evaluated interleukins.ConclusionIn the current study, we
demonstrated that the direct exposure of a complex tetra-culture alveolar model to
different types of AgNMs at ALI induces shape- and size-specific biological
responses. From the three AgNMs tested, AgNWs were the most potent in inducing
biological alterations. Starting from 50ng/cm(2), a dose representative for an
acute exposure in a high exposure occupational setting, AgNWs induced prominent
changes indicative for a pro-inflammatory response. Even though the acute responses
towards a dose representative for a full-lifetime exposure were also evaluated,
chronic exposure scenarios at low dose are still unquestionably needed to reveal
the human health impact of AgNMs during realistic conditions.
AN - WOS:000463602100001
AU - Fizesan, I.
AU - Cambier, S.
AU - Moschini, E.
AU - Chary, A.
AU - Nelissen, I.
AU - Ziebel, J.
AU - Audinot, J. N.
AU - Wirtz, T.
AU - Kruszewski, M.
AU - Pop, A.
AU - Kiss, B.
AU - Serchi, T.
AU - Loghin, F.
AU - Gutleb, A. C.
C7 - 14
DA - APR 2
DO - 10.1186/s12989-019-0297-1
PY - 2019
SN - 1743-8977
ST - In vitro exposure of a 3D-tetraculture representative for the alveolar
T2 - PARTICLE AND FIBRE TOXICOLOGY
TI - In vitro exposure of a 3D-tetraculture representative for the alveolar
VL - 16
ID - 6227
ER -
TY - JOUR
AB - The study was focused on the phytochemicals-mediated biosynthesis of silver
nanoparticles using leaf extracts and infusions from Cynara scolymus. To identify
the antioxidant activity and total phenolic content, the 1,1-diphenyl-1-
picrylhydrazyl and Folin–Ciocalteau methods were applied, respectively. The
formation and stability of the reduced silver ions were monitored by UV–vis
spectrophotometer. The particle sizes of the silver nanoparticles were
characterised using the dynamic light scattering technique and scanning electron
microscope. The phase composition of the obtained silver nanoparticles was
characterised by X-ray diffraction. The silver nanoparticles suspension, artichoke
infusion, and silver ions were separately tested towards potential cytotoxicity and
pro-inflammatory effect using mouse fibroblasts and human monocytes cell line,
respectively. The total phenolic content and antioxidant activity of ethanol
extract and infusion were found significantly higher as compared to aqueous extract
and infusion. The UV–visible spectrophotometric analysis revealed the presence of
the characteristic absorption band of the Ag nanoparticles. Moreover, it was found
that with the increasing volume of plant extract, the average size of particles was
increased. Biocompatibility results evidently showed that silver nanoparticles do
not induce monocyte activation, however in order to avoid their cytotoxicity
suspension at a concentration <2 ppm should be applied. © The Institution of
Engineering and Technology 2019.
AU - Florkiewicz, W.
AU - Malina, D.
AU - Pluta, K.
AU - Rudnicka, K.
AU - Gajewski, A.
AU - Olejnik, E.
AU - Tyliszczak, B.
AU - Sobczak-Kupiec, A.
DB - Scopus
DO - 10.1049/iet-nbt.2018.5357
IS - 7
KW - Animals
Antioxidants
Cell Proliferation
Cell Survival
Cells, Cultured
Cynara scolymus
Fibroblasts
Humans
Immune System
Materials Testing
Metal Nanoparticles
Mice
Monocytes
Phytochemicals
Plant Extracts
Plant Leaves
Silver
Toxicity Tests
Biochemistry
Biocompatibility
Cell culture
Cytotoxicity
Light scattering
Metal ions
Metal nanoparticles
Plant extracts
Spectrophotometers
Suspensions (fluids)
antioxidant
metal nanoparticle
phytochemical
plant extract
silver
Aqueous extracts
Characteristic absorption
Dynamic light scattering technique
Monocyte activation
Mouse-fibroblasts
Spectrophotometric analysis
Total phenolic content
animal
artichoke
cell culture
cell proliferation
cell survival
chemistry
cytology
drug effect
fibroblast
human
immune system
materials testing
metabolism
monocyte
mouse
physiology
plant leaf
synthesis
toxicity testing
M3 - Article
PY - 2019
SP - 726-735
ST - Assessment of cytotoxicity and immune compatibility of phytochemicals-
mediated biosynthesised silver nanoparticles using Cynara scolymus
T2 - IET Nanobiotechnology
TI - Assessment of cytotoxicity and immune compatibility of phytochemicals-
mediated biosynthesised silver nanoparticles using Cynara scolymus
85072701892&doi=10.1049%2fiet-
nbt.2018.5357&partnerID=40&md5=c231e797ccfbde9d791f3002939400f4
VL - 13
ID - 5413
ER -
TY - JOUR
AB - Despite the potential antimicrobial activity of metallic nanoparticles, the
increasing concerns about nanosafety have been holding back the use of these
materials in therapeutics and biomedical devices. In the last years, several
studies called attention to metallic nanoparticles toxicity. In the most part of in
vitro studies performed with mammalian cells, metallic NPs reduced cell viability
and induced genotoxicity and inflammatory responses. Bimetallic NPs have attracted
great attention because they present distinct and even more advanced
characteristics when compared to nanoparticles formed by a single metal. Recently,
bimetallic NPs have emerged as an alternative to improve the antimicrobial activity
of metallic nanoparticles, aiming at the broadening of the action spectrum and the
reduction of the toxicity. However, the biocompatibility of bimetallic
nanoparticles has been demonstrated only by in vitro studies. In the present work,
the toxicity of AuPt nanoparticles was addressed both in vitro and in vivo. In
addition, the antimicrobial activity of AuPt bimetallic nanoparticles has been
evaluated in comparison with Au and Ag nanoparticles. The nanoparticles were
characterized by ultraviolet-visible spectroscopy, dynamic light scattering,
transmission electron microscopy, inductively coupled plasma optical emission
spectroscopy, and X-ray diffraction. The antimicrobial activity was studied against
Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. The toxicity
of nanoparticles was evaluated in vitro by analyzing their toxicity against human
fibroblast cells (HS68 cell line) and in vivo by embryonic toxicity test in
zebrafish (Danio rerio). The results confirmed the intrinsic antimicrobial activity
of the three types of nanoparticles but different toxicity. Bimetallic
nanoparticles showed enhanced antimicrobial activity in comparison with Au
nanoparticles but lower antimicrobial activity compared with Ag nanoparticles.
However, AuPt nanoparticles showed great advantage over Ag nanoparticles due to the
absence of cytotoxicity and lower toxicity in vivo. © 2019, Springer Nature B.V.
AU - Formaggio, D. M. D.
AU - de Oliveira Neto, X. A.
AU - Rodrigues, L. D. A.
AU - de Andrade, V. M.
AU - Nunes, B. C.
AU - Lopes-Ferreira, M.
AU - Ferreira, F. G.
AU - Wachesk, C. C.
AU - Camargo, E. R.
AU - Conceição, K.
AU - Tada, D. B.
C7 - 244
DB - Scopus
DO - 10.1007/s11051-019-4683-2
IS - 11
Bimetallic nanoparticles
Environmental and health effects
Gold nanoparticles
Toxicity
Zebrafish
Bacteria
Binary alloys
Biocompatibility
Cell culture
Cells
Cytotoxicity
Inductively coupled plasma
Light scattering
Light transmission
Mammals
Metal nanoparticles
Metals
Optical emission spectroscopy
Platinum alloys
gold nanoparticle
gold platinum nanoparticle
platinum nanoparticle
unclassified drug
Human fibroblast cells
Inductively coupled plasma-optical emission spectroscopy
animal experiment
Article
biocompatibility
biological activity
Candida albicans
controlled study
embryo
fibroblast cell line
human
human cell
in vitro study
in vivo study
nonhuman
priority journal
toxicity testing
X ray diffraction
zebra fish
M3 - Article
PY - 2019
ST - In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles
TI - In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles
85075394946&doi=10.1007%2fs11051-019-4683-
2&partnerID=40&md5=f52e7e23b4df7dbd734f8b0ef63406d3
VL - 21
ID - 5392
ER -
TY - JOUR
AB - Despite the potential antimicrobial activity of metallic nanoparticles, the
increasing concerns about nanosafety have been holding back the use of these
materials in therapeutics and biomedical devices. In the last years, several
studies called attention to metallic nanoparticles toxicity. In the most part of in
vitro studies performed with mammalian cells, metallic NPs reduced cell viability
and induced genotoxicity and inflammatory responses. Bimetallic NPs have attracted
great attention because they present distinct and even more advanced
characteristics when compared to nanoparticles formed by a single metal. Recently,
bimetallic NPs have emerged as an alternative to improve the antimicrobial activity
of metallic nanoparticles, aiming at the broadening of the action spectrum and the
reduction of the toxicity. However, the biocompatibility of bimetallic
nanoparticles has been demonstrated only by in vitro studies. In the present work,
the toxicity of AuPt nanoparticles was addressed both in vitro and in vivo. In
addition, the antimicrobial activity of AuPt bimetallic nanoparticles has been
evaluated in comparison with Au and Ag nanoparticles. The nanoparticles were
characterized by ultraviolet-visible spectroscopy, dynamic light scattering,
transmission electron microscopy, inductively coupled plasma optical emission
spectroscopy, and X-ray diffraction. The antimicrobial activity was studied against
Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. The toxicity
of nanoparticles was evaluated in vitro by analyzing their toxicity against human
fibroblast cells (HS68 cell line) and in vivo by embryonic toxicity test in
zebrafish (Danio rerio). The results confirmed the intrinsic antimicrobial activity
of the three types of nanoparticles but different toxicity. Bimetallic
nanoparticles showed enhanced antimicrobial activity in comparison with Au
nanoparticles but lower antimicrobial activity compared with Ag nanoparticles.
However, AuPt nanoparticles showed great advantage over Ag nanoparticles due to the
absence of cytotoxicity and lower toxicity in vivo.
AN - WOS:000497742600001
AU - Formaggio, D. M. D.
AU - Neto, X. A. D.
AU - Rodrigues, L. D. A.
AU - de Andrade, V. M.
AU - Nunes, B. C.
AU - Lopes-Ferreira, M.
AU - Ferreira, F. G.
AU - Wachesk, C. C.
AU - Camargo, E. R.
AU - Conceicao, K.
AU - Tada, D. B.
C7 - 244
DA - NOV
DO - 10.1007/s11051-019-4683-2
IS - 11
PY - 2019
SN - 1388-0764
1572-896X
ST - In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles
TI - In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles
VL - 21
ID - 6137
ER -
TY - JOUR
AB - Objective. Ischemia-reperfusion injury (IRI) produces systemic inflammation
with the potential for causing organ failure in tissues peripheral to the initial
site of injury. We speculate that treatment strategies that dampen inflammation may
be therapeutically beneficial to either the initial site of injury or peripheral
organs. To test this, we evaluated the impact of FTY720-induced sequestration of
circulating mature lymphocytes on renal IRI and secondary organ injury. Methods. A
microvascular clamp was surgically placed around the left renal pedicle of
anesthetized male Sprague-Dawley rats with either vehicle or FTY720 treatment
(0.3mg/kg) intravenously injected after 15min of ischemia. Blood flow was restored
after 60min. Cohorts of anesthetized rats were euthanized at 6, 24, or 72hrs with
tissue samples collected for analysis. Results. FTY720 treatment resulted in
profound T lymphocyte reduction in peripheral blood. Histopathologic examination,
clinical chemistries, and gene transcript expression measurements revealed that
FTY720 treatment reduced hepatocellular degeneration, reduced serum markers of
liver injury (ALT/AST), and reduced the expression of gene targets associated with
IRI. Conclusion. These findings support an anti-inflammatory effect of FTY720 in
the liver where the expression of genes associated with apoptosis, chemotaxis, and
the AP-1 transcription factor was reduced. Findings presented here provide the
basis for future studies evaluating FTY720 as a potential therapeutic agent to
treat complications resulting from renal IRI.
AN - WOS:000463696100001
AU - Foster, A. D.
AU - Vicente, D.
AU - Clark, N.
AU - Leonhardt, C.
AU - Elster, E. A.
AU - Davis, T. A.
AU - Bradley, M. J.
C7 - 3496836
DO - 10.1155/2019/3496836
PY - 2019
SN - 0962-9351
1466-1861
ST - FTY720 Effects on Inflammation and Liver Damage in a Rat Model of Renal
Ischemia-Reperfusion Injury
T2 - MEDIATORS OF INFLAMMATION
TI - FTY720 Effects on Inflammation and Liver Damage in a Rat Model of Renal
Ischemia-Reperfusion Injury
VL - 2019
ID - 6675
ER -
TY - JOUR
AB - Noble metal nanoparticles (NP) with intrinsic antiangiogenic, antibacterial,
and anti-inflammatory properties have great potential as potent chemotherapeutics,
due to their unique features, including plasmonic properties for application in
photothermal therapy, and their capability to slow down the migration/invasion
speed of cancer cells and then suppress metastasis. In this work, gold (Au), silver
(Ag), and palladium (Pd) NP were synthesized by a green redox chemistry method with
the reduction of the metal salt precursor with glucose in the presence of
polyvinylpyrrolidone (PVP) as stabilizing and capping agent. The physicochemical
properties of the PVP-capped NP were investigated by UV-visible (UV-vis) and
attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopies,
dynamic light scattering (DLS), and atomic force microscopy (AFM), to scrutinize
the optical features and the interface between the metal surface and the capping
polymer, the hydrodynamic size, and the morphology, respectively. Biophysical
studies with model cell membranes were carried out by using laser scanning confocal
microscopy (LSM) with fluorescence recovery after photobleaching (FRAP) and
fluorescence resonance energy transfer (FRET) techniques. To this purpose,
artificial cell membranes of supported lipid bilayers (SLBs) made with 1-palmitoyl-
2-oleoyl-sn-glycerol-3-phosphocholine (POPC) dye-labeled with 7-nitro-2-1,3-
benzoxadiazol-4-yl (NBD, FRET donor) and/or lissamine rhodamine B sulfonyl (Rh,
FRET acceptor) were prepared. Proof-of-work in vitro cellular experiments were
carried out with prostate cancer cells (PC-3 line) in terms of cytotoxicity, cell
migration (wound scratch assay), NP cellular uptake, and cytoskeleton actin
perturbation. © 2023 by the authors.
AU - Foti, A.
AU - Calì, L.
AU - Petralia, S.
AU - Satriano, C.
C7 - 1624
DB - Scopus
DO - 10.3390/nano13101624
IS - 10
KW - FRAP
FRET
gold nanoparticles
palladium nanoparticles
plasmonic nanoparticles
prostate cancer cells
supported lipid bilayers
M3 - Article
PY - 2023
ST - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Study at the Hybrid Interface with Biomimetic Cell Membranes and In Vitro Cell
Models
T2 - Nanomaterials
TI - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Models
85160446358&doi=10.3390%2fnano13101624&partnerID=40&md5=f9e3eb01bfe5971d8c47c1533af
afa65
VL - 13
ID - 4975
ER -
TY - JOUR
AB - Noble metal nanoparticles (NP) with intrinsic antiangiogenic, antibacterial,
and anti-inflammatory properties have great potential as potent chemotherapeutics,
due to their unique features, including plasmonic properties for application in
photothermal therapy, and their capability to slow down the migration/invasion
speed of cancer cells and then suppress metastasis. In this work, gold (Au), silver
(Ag), and palladium (Pd) NP were synthesized by a green redox chemistry method with
the reduction of the metal salt precursor with glucose in the presence of
polyvinylpyrrolidone (PVP) as stabilizing and capping agent. The physicochemical
properties of the PVP-capped NP were investigated by UV-visible (UV-vis) and
attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopies,
dynamic light scattering (DLS), and atomic force microscopy (AFM), to scrutinize
the optical features and the interface between the metal surface and the capping
polymer, the hydrodynamic size, and the morphology, respectively. Biophysical
studies with model cell membranes were carried out by using laser scanning confocal
microscopy (LSM) with fluorescence recovery after photobleaching (FRAP) and
fluorescence resonance energy transfer (FRET) techniques. To this purpose,
artificial cell membranes of supported lipid bilayers (SLBs) made with 1-palmitoyl-
2-oleoyl-sn-glycerol-3-phosphocholine (POPC) dye-labeled with 7-nitro-2-1,3-
benzoxadiazol-4-yl (NBD, FRET donor) and/or lissamine rhodamine B sulfonyl (Rh,
FRET acceptor) were prepared. Proof-of-work in vitro cellular experiments were
carried out with prostate cancer cells (PC-3 line) in terms of cytotoxicity, cell
migration (wound scratch assay), NP cellular uptake, and cytoskeleton actin
perturbation.
AN - WOS:000997232600001
AU - Foti, A.
AU - Cali, L.
AU - Petralia, S.
AU - Satriano, C.
C7 - 1624
DA - MAY 12
DO - 10.3390/nano13101624
IS - 10
PY - 2023
SN - 2079-4991
ST - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Models
T2 - NANOMATERIALS
TI - Green Nanoformulations of Polyvinylpyrrolidone-Capped Metal Nanoparticles: A
Models
VL - 13
ID - 6202
ER -
TY - JOUR
AB - Recruitment of cells and mediators is altered during impaired wound healing,
thereby delaying this process. To overcome this problem, the correlation of wound
healing in older rats, and the impact of different molecular weight of hyaluronan
without silver nanoparticles; (low-HA1), (High-HA2), (Medium- HA3) and with silver
nanoparticles (High-HA4) is investigated. The superior HA were selected to be
further investigated onto diabetic wounds. Our results pointed to a marked
deficiency in wounds granulation in older rats, which was accompanied with
impairment of healing process. In older rats group treated with HA2 or HA4,
granulation and dermal construction were improved. Furthermore, the number of
pathogenic bacteria on wounds was declined throughout the first 24 h by HA2 and
HA4. The wound size in HA4-treated older rats was significantly smaller than that
in other HA1, HA2 or HA3-treated older ones. Also, diabetes impaired the level of
inflammatory cytokine, in diabetic model. On contrary, HA4 was found to normalize
the level of inflammatory cytokine, in the diabetic model. Furthermore, HA4 was
found to recover all oxidative and toxicity markers in diabetic models. This data
confirms the critical role of HA4 to improve granulation and inflammatory mediators
in impaired older and diabetic rat wound healing. © 2016 Elsevier B.V.
AU - Fouda, M. M. G.
AU - Abdel-Mohsen, A. M.
AU - Ebaid, H.
AU - Hassan, I.
AU - Al-Tamimi, J.
AU - Abdel-Rahman, R. M.
AU - Metwalli, A.
AU - Alhazza, I.
AU - Rady, A.
AU - El-Faham, A.
AU - Jancar, J.
DB - Scopus
DO - 10.1016/j.ijbiomac.2016.05.021
KW - Animal models
Hyaluronan
Wound healing
Animals
Humans
Hyaluronic Acid
Inflammation
Metal Nanoparticles
Molecular Weight
Rats
Silver
Wound Healing
cytokine
hyaluronic acid
silver nanoparticle
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
bacterial count
controlled study
diabetes mellitus
in vivo study
inflammation
male
molecular weight
nonhuman
oxidative stress
rat
tissue regeneration
wound
wound healing
wound healing impairment
animal
chemistry
complication
drug effects
human
microbiology
pathology
M3 - Article
PY - 2016
SP - 582-591
ST - Wound healing of different molecular weight of hyaluronan; in-vivo study
TI - Wound healing of different molecular weight of hyaluronan; in-vivo study
84969262169&doi=10.1016%2fj.ijbiomac.2016.05.021&partnerID=40&md5=15662c9dc076c52ee
aa6852b5b45f854
VL - 89
ID - 5546
ER -
TY - JOUR
AB - Nitric oxide (NO) is important for the regulation of a number of diverse
biological processes, including vascular tone, neurotransmission, inflammatory cell
responsiveness, defence against invading pathogens and wound healing. Transition
metal exchanged zeolites are nanoporous materials with high-capacity storage
properties for gases such as NO. The NO stores are liberated upon contact with
aqueous environments, thereby making them ideal candidates for use in biological
and clinical settings. Here, we demonstrate the NO release capacity and powerful
bactericidal properties of a novel NO-storing Zn2+-exchanged zeolite material at a
50 wt.% composition in a polytetrafluoroethylene polymer. Further to our published
data showing the anti-thrombotic effects of a similar NO-loaded zeolite, this study
demonstrates the antibacterial properties of NO-releasing zeolites against
clinically relevant strains of bacteria, namely Gram-negative Pseudomonas
aeruginosa and Gram-positive methicillin-sensitive and methicillin-resistant
Staphylococcus aureus and Clostridium difficile. Thus our study highlights the
potential of NO-loaded zeolites as biocompatible medical device coatings with anti-
infective properties. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All
rights reserved.
AN - WOS:000276013500034
AU - Fox, S.
AU - Wilkinson, T. S.
AU - Wheatley, P. S.
AU - Xiao, B.
AU - Morris, R. E.
AU - Sutherland, A.
AU - Simpson, A. J.
AU - Barlow, P. G.
AU - Butler, A. R.
AU - Megson, I. L.
AU - Rossi, A. G.
DA - APR
DO - 10.1016/j.actbio.2009.10.038
IS - 4
PY - 2010
SN - 1742-7061
SP - 1515-1521
ST - NO-loaded Zn2+-exchanged zeolite materials: A potential bifunctional anti-
bacterial strategy
TI - NO-loaded Zn2+-exchanged zeolite materials: A potential bifunctional anti-
bacterial strategy
VL - 6
ID - 6724
ER -
TY - JOUR
AB - The nanoparticles are known to reduce toxicity, enhance bioactivity and
improve targeting cells. The metal and metal oxide nanoparticles such as gold,
silver, zinc oxide, zirconium oxide, copper oxide, copper sulfide, selenium,
hydroxyl apatite, and titanium oxide were used in several biomedical applica-tions
especially diagnosis of cancer, etc. The selenium nanoparticles (SeNPs) are
explored due toits unique characteristics and various known therapeutic benefits
such as anti bacterial, anti fungal and anti cancer activities. The selenium
nanoparticles actively involved in the free radical scavenging activity and anti
inflammatory activity. The antioxidant activities and reducing risk of cancer.In
this present study, Cynodondactylon or Bermuda grass mediated synthesis of selenium
nanoparticles and it was characterized using UV-vis spectrophotometer. And its
antioxidant effect was analysed using DPPH assay. The plant extract of
Cynodondactylon was prepared using distilled water and mixed with the sodium
selenite solution. The solution was kept in a magnetic stirrer at 100 RPM for
constant stirring while being observed for any colour change. The results showed
that there was a significant antioxidant activity and peak confirm the selenium
nanoparticles formation. Finally we conclude that the selenium nanoparticles can be
used in various drug designing aspects in future. © 2020 International Journal of
Research in Pharmaceutical Sciences. All rights reserved.
AU - Francis, T.
AU - Preejitha, V. B.
AU - Rajeshkumar, S.
DB - Scopus
DO - 10.26452/ijrps.v11i4.3301
IS - 4
KW - Antioxidant activity
Green synthesis
Selenium nanoparticles
ascorbic acid
sodium selenite
antifungal activity
arrow root
Article
biocompatibility
Cynodon dactylon
diabetic complication
drug development
liver cirrhosis
nephrotoxicity
M3 - Article
PY - 2020
SP - 6221-6226
ST - Antioxidant activity of cynodon dactylon mediated selenium nanoparticles
T2 - International Journal of Research in Pharmaceutical Sciences
TI - Antioxidant activity of cynodon dactylon mediated selenium nanoparticles
85092203654&doi=10.26452%2fijrps.v11i4.3301&partnerID=40&md5=c00c41651bea474a784938
8a067e028b
VL - 11
ID - 5328
ER -
TY - JOUR
AB - Background: Nanoparticles are widely used in different technological fields,
one of which is medicine. Because of their antibacterial properties, silver
nanoparticles (AgNPs) are used in several types of wound dressings for the
treatment of burns and nonhealing wounds, but their influence on each component of
the wound-healing process remains unclear. In the present study, we evaluated the
effects of AgNPs on normal human dermal fibroblasts (NHDFs) and normal human
epidermal keratinocytes (NHEKs). Both cell types are important for wound healing,
including with regard to inflammation, proliferation and tissue remodeling. Each
phase of wound healing can be characterized by the secretion of cytokines,
chemokines and growth factors. Methods: The production of inflammatory parameters
(tumor necrosis factor alpha [TNF-alpha], interleukin-6 [IL-6], IL-8 and IL-12 and
cyclooxygenase-2 [COX-2]), angiogenesis parameters (vascular endothelial growth
factor [VEGF], granulocyte macrophage colony-stimulating factor) and matrix
metalloproteinases (MMP-1, MMP-2, MMP-3 and MMP-9) by NHDFs and NHEKs were examined
by ELISA or Western blot after 24 and 48 hours of incubation with AgNPs. Results:
We found that AgNPs decreased some inflammatory cytokines (TNF-alpha and IL-12) and
growth factors (VEGF) that were produced by NHDFs and NHEKs after 24 and 48 hours
and decreased the expression of COX-2 after 24 hours but only at the highest
concentration of AgNPs (25 parts per million). Conclusions: The results indicate
that NHEKs are more susceptible to the application of AgNPs than NHDFs, and AgNPs
may be useful for medical applications for the treatment of wounds.
AN - WOS:000388385600002
AU - Frankova, J.
AU - Pivodova, V.
AU - Vagnerova, H.
AU - Juranova, J.
AU - Ulrichova, J.
DA - APR-JUN
DO - 10.5301/jabfm.5000268
IS - 2
PY - 2016
SN - 2280-8000
SP - E137-E142
ST - Effects of silver nanoparticles on primary cell cultures of fibroblasts and
keratinocytes in a wound-healing model
T2 - JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS
TI - Effects of silver nanoparticles on primary cell cultures of fibroblasts and
keratinocytes in a wound-healing model
VL - 14
ID - 5986
ER -
TY - JOUR
AB - Neutrophil surveillance is central to nanoparticle clearance. Silver
nanoparticles (AgNP) have numerous uses, however conflicting evidence exists as to
their impact on neutrophils and whether they trigger damaging inflammation.
Neutrophil's importance in innate defence and regulating immune networks mean it's
essential we understand AgNP's impact on neutrophil function. Human neutrophil
viability following AgNP or Ag Bulk treatment was analysed by flow cytometry and
AnV/PI staining. Whilst AgNP exposure did not increase the total number of
apoptotic neutrophils, the number of late apoptotic neutrophils was increased,
suggesting AgNP increase transit through apoptosis. Mature
(CD16bright/CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic
(CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil
preparations. AgNP exposure significantly reduced CD62L staining of
CD16bright/CD62Lbright neutrophils, and increased CD16 staining of
CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and
maturation, respectively. AgNP exposure dramatically increased IL-8, yet not
classical pro-inflammatory cytokine release, suggesting AgNP triggers neutrophil
activation, without pro-inflammation or damaging, necrotic cell death. For the
first time, we show AgNPs differentially affect distinct sub-populations of
circulating human neutrophils; activating mature neutrophils with the emergence of
CD16bright/CD62Ldim neutrophils. This may stimulate particle clearance without
harmful inflammation, challenging previous assumptions that silver nanomaterials
induce neutrophil toxicity and damaging inflammatory responses. © 2018 The
Author(s).
AU - Fraser, J. A.
AU - Kemp, S.
AU - Young, L.
AU - Ross, M.
AU - Prach, M.
AU - Hutchison, G. R.
AU - Malone, E.
C7 - 7506
DB - Scopus
DO - 10.1038/s41598-018-25854-2
IS - 1
KW - Cell Survival
Flow Cytometry
Humans
Interleukin-8
L-Selectin
Metal Nanoparticles
Neutrophil Activation
Neutrophils
Receptors, IgG
Silver
Up-Regulation
CXCL8 protein, human
Fc receptor
interleukin 8
L selectin
metal nanoparticle
SELL protein, human
silver
cell survival
cytology
drug effect
flow cytometry
human
immunology
metabolism
neutrophil
upregulation
M3 - Article
PY - 2018
ST - Silver nanoparticles promote the emergence of heterogeneic human neutrophil
sub-populations
TI - Silver nanoparticles promote the emergence of heterogeneic human neutrophil
sub-populations
85047013524&doi=10.1038%2fs41598-018-25854-
2&partnerID=40&md5=1d42ecd330d255342cd8bcf85b06d64e
VL - 8
ID - 5365
ER -
TY - CONF
AB - Biocompatibility is the ability of a material appropriate trigger a
biological response, when applied to the body, without causing a chronic
inflammatory reaction, foreign body reaction or toxicity, is related to the
interaction of the cell/biomaterial. A few materials, if any, are completely inert
from the physiological point of view since, most of the components with a variety
of potential toxic or irritating. In addition, chemical reactions during cure of
the material may also produce undesirable effects. In order to increase knowledge
about the characteristics and properties of materials and their interaction with
the biological environment, this study aimed, through literature review, guide and
inform didactically professionals and academics on the importance of
biocompatibility of restorative materials more direct use in dental practice:
silver amalgam, composite resins and glass ionomer cements. It was concluded that,
among the restorative materials studied, the glass ionomer cement showed the best
characteristics and properties that confirm its biocompatibility in dental
practice. © (2015) Trans Tech Publications, Switzerland.
AU - Freire, W. P.
AU - Fook, M. V. L.
AU - Barbosa, E. F.
AU - dos S. Araújo, C.
AU - Barbosa, R. C.
AU - Pinheiro, Í M. F.
DB - Scopus
DO - 10.4028/www.scientific.net/MSF.805.19
Biomaterial
Restorative materials
Artificial organs
Biomaterials
Cements
Dental alloys
Dental materials
Glass
Ionomers
Biological environments
Biological response
Dental restorative materials
Glass ionomer cement
Undesirable effects
Toxic materials
PY - 2015
SP - 19-25
ST - Biocompatibility of dental restorative materials
T2 - Materials Science Forum
TI - Biocompatibility of dental restorative materials
84922222528&doi=10.4028%2fwww.scientific.net
%2fMSF.805.19&partnerID=40&md5=7ed8fafa2b1c7c15371cc8226f36b884
VL - 805
ID - 5585
ER -
TY - JOUR
AB - PURPOSE: To evaluate the effects of maternal remote ischemic preconditioning
(IPCr) in the colonic mucosa of newborn rats subjected to hypoxia and
reoxygenation. METHODS: Newborn Wistar rats were divided into three groups. Control
Group (CG), Hypoxia and Reoxygenation Group (HRG) and Remote Ischemic
Preconditioning Group (IPCrG). Hypoxia and reoxygenation was performed 2x per day,
with an interval of 6 hours, on the 1st, 2nd and 3rd days of life, with 10 minutes
of CO2 at 100%, followed by 10 minutes O2 at 100%(HRG/IPCrG). The maternal IPCr was
performed 24 hours before delivery by applying a rubber band tourniquet to the left
hind limb (IPCrG). Segments of the colon underwent histological (HE) and
immunohistochemical analysis for caspase-3 and COX - 2. RESULTS: The histological
findings showed no intestinal mucosal damage in the CG group and severe lesions in
HRG that was attenuated in the IPCrG (p<0.05). The expression of the apoptotic
cells was lower in the HRG group than in the CG and IPCrG. The COX-2 expression was
intense in HRG and attenuated in the IPCrG (p<0.05). CONCLUSIONS: Maternal IPCr
protected the colonic mucosa of newborn rats subjected to hypoxia and
reoxygenation, reducing the morphological alterations and inflammatory response. It
ameliorates the occurrence of apoptosis, keeping the physiological process of
renewal and regeneration in the epithelial lining of the colonic mucosa. .
AD - Freitas, Maria Andréia Lopes de
Federal University of Sao Paulo. Paulista School of Medicinev. Recife. BR
Gomes, Rúdnei de Oliveira Luciano
Soares, Bruno Leonardo de Freitas
Artigiani Neto, Ricardo
Montero, Edna Frasson de Souza
Martins, José Luiz
AU - Freitas, Maria Andréia Lopes de
AU - Gomes, Rúdnei de Oliveira Luciano
AU - Soares, Bruno Leonardo de Freitas
AU - Artigiani Neto, Ricardo
AU - Montero, Edna Frasson de Souza
AU - Martins, José Luiz
C1 - 20140717
DA - 2014/07
DB - LILACS
DO - 10.1590/S0102-86502014000700005
IS - 7
KW - Apoptosis
Colon
Enterocolitis
Necrotizing
Rats
LA - en
PY - 2014
SN - 0102-8650
SP - 438-444
ST - Effects of maternal ischemic preconditioning in the colon of newborn rats
submitted to hypoxia-reoxygenation insult
T2 - Acta cir. bras
TI - Effects of maternal ischemic preconditioning in the colon of newborn rats
submitted to hypoxia-reoxygenation insult
86502014000700438
VL - 29
ID - 4940
ER -
TY - CONF
AB - Background: Some of the views contrasting the beneficial and toxic effects of
antimicrobials upon wound healing remain controversial. Objective: To assess the
clinical relevance of histological findings following antimicrobial applications on
chronic leg ulcers. Method: The present study was performed in three parallel
groups of 17 patients suffering from at least 2 similar chronic leg ulcers.
Clinical planimetric assessments were performed before and after 3 and 6 weeks of
treatment using hydrocolloid dressings. In addition, 1 ulcer in each patient
received applications of povidone-iodine (PVP-I), silver sulfadiazine or
chlorhexidine digluconate. Histological examinations were made at inclusion and
after the 6-week therapy. Time to healing was also recorded. Results: At entry in
the study, fibroblasts, macrophages, neutrophils and vessels were abundant in the
ulcers. In addition, focal necrotizing vasculitis was related to the
microbiological load. Compared to the control lesions, both the healing rate and
time to healing of the leg ulcers showed a modest improvement at the sites
receiving silver sulfadiazine (2-7%) or chlorhexidine digluconate (-1 to 5%). By
contrast, PVP-I increased significantly the healing rate (4-18%, p < 0.01), and
time to healing was reduced by 2-9 weeks (p < 0.01). The 3 antimicrobials decreased
the bacterial density, and the vascular margination and migration of inflammatory
cells, thus abating the vasculitic changes. PVP-I applications did not alter the
microvessels and did not significantly reduce the density in dendrocytes and
fibroblasts. By contrast, both silver sulfadiazine and chorhexidine digluconate
appeared to alter the superficial microsvasculature including the dendrocyte
population. Conclusion: Although topical antimicrobials may apparently achieve
almost similar activity on the bacterial load inside chronic leg ulcers, the
toxicity upon host cells was different among these agents. PVP-I appeared to be an
efficient compound in these respects exhibiting a positive and relevant clinical
effect. Copyright © 2002 S. Karger AG, Basel.
AU - Fumal, I.
AU - Braham, C.
AU - Paquet, P.
AU - Piérard-Franchimont, C.
AU - Piérard, G. E.
DB - Scopus
DO - 10.1159/000057729
ET - SUPPL. 1
KW - Antiseptic
Bacteria
Cytotoxicity
Leg ulcer
Wound healing
Anti-Infective Agents, Local
Chlorhexidine
Humans
Leg Ulcer
Middle Aged
Povidone-Iodine
Silver Sulfadiazine
Wound Healing
antiinfective agent
chlorhexidine gluconate
iso betadine dermique
povidone iodine
sulfacetamide
sulfadiazine
adult
antimicrobial therapy
cell density
cell migration
clinical trial
conference paper
controlled study
cytotoxicity
dose response
drug effect
histology
human
human tissue
inflammatory cell
leg ulcer
macrophage
microflora
microvasculature
necrotizing arteritis
neutrophil
priority journal
skin cell
skin fibroblast
wound dressing
wound healing
PY - 2002
SP - 70-74
ST - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
impaired by a polymicrobial flora: A proof-of-concept study
T2 - Dermatology
TI - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
0036092834&doi=10.1159%2f000057729&partnerID=40&md5=e25513e9fabe22a97d9aac685875269
4
VL - 204
ID - 5765
ER -
TY - JOUR
AB - Background: Some of the views contrasting the beneficial and toxic effects of
antimicrobials upon wound healing remain controversial. Objective: To assess the
clinical relevance of histological findings following antimicrobial applications on
chronic leg ulcers. Method. The present study was performed in three parallel
groups of 17 patients suffering from at least 2 similar chronic leg ulcers.
Clinical planimetric assessments were performed before and after 3 and 6 weeks of
treatment using hydrocolloid dressings. In addition, 1 ulcer in each patient
received applications of povidone-iodine (PVP-I), silver sulfadiazine or
chlorhexidine digluconate. Histological examinations were made at inclusion and
after the 6-week therapy. Time to healing was also recorded. Results: At entry in
the study, fibroblasts, macrophages, neutrophils and vessels were abundant in the
ulcers. In addition, focal necrotizing vasculitis was related to the
microbiological load. Compared to the control lesions, both the healing rate and
time to healing of the leg ulcers showed a modest improvement at the sites
receiving silver sulfadiazine (2-7%) or chlorhexidine digluconate (-1 to 5%). By
contrast, PVP-I increased significantly the healing rate (4-18%, p<0.01), and time
to healing was reduced by 2-9 weeks (p<0.01). The 3 antimicrobials decreased the
bacterial density, and the vascular margination and migration of inflammatory
cells, thus abating the vasculitic changes. PVP-I applications did not alter the
microvessels and did not significantly reduce the density in dendrocytes and
fibroblasts. By contrast, both silver sulfadiazine and chorhexidine digluconate
appeared to alter the superficial microsvasculature including the dendrocyte
population. Conclusion: Although topical antimicrobials may apparently achieve
almost similar activity on the bacterial load inside chronic leg ulcers, the
toxicity upon host cells was different among these agents. PVP-I appeared to be an
efficient compound in these respects exhibiting a positive and relevant clinical
effect. Copyright (C) 2002 S. Karger AG, Basel.
AN - WOS:000175633100014
AU - Fumal, I.
AU - Braham, C.
AU - Paquet, P.
AU - Pierard-Franchimont, C.
AU - Pierard, G. E.
DO - 10.1159/000057729
PY - 2002
SN - 1018-8665
1421-9832
SP - 70-74
ST - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
T2 - DERMATOLOGY
TI - The beneficial toxicity paradox of antimicrobials in leg ulcer healing
VL - 204
ID - 6413
ER -
TY - JOUR
AB - Objective: Previous studies have shown that silver formulations coated onto
implantable materials retard bacterial colonization and reduce the incidence of
catheter related infections. The objective of this study was to assess the
histologic effects of sputter-coated silver/silicone implants on host tissue.
Design: Sputter silver-coated silicone peritoneal dialysis catheter segments with
and without Dacron cuffs were implanted in the subcutaneous fat and muscle in 4
pigs. Noncoated implants served as controls. The specimens were retrieved at 1, 2,
3, 4, 7, 8, 9, 10, 12, and 27 weeks. Experimental Animals: Four 6-week-old male
Yorkshire-Landrace pigs (5-6 kg) were used. Main Outcome Measures: Histologic
parameters evaluated included the degree of inflammation, the number of giant
cells, the extent of silver particulate inclusions, and the thickness of the
capsules. All specimens were evaluated by a single blinded pathologist.
Microbiologic analyses were also performed. Results: The silver-coated catheters
were associated with less inflammation than were the noncoated catheters, both in
fat and muscle (p = 0.04). The number of giant cells was also lower around the
silver-coated than the noncoated catheters, which were implanted in subcutaneous
fat (p < 0.05). Particulate inclusions compatible with silver or silver oxide were
observed only in tissue around silver-coated implants (p < 0.0001). The thickness
of the capsules and the extent of the inflammatory zones were not significantly
different. There was no evidence of infection-related changes. Conclusions: These
data suggest that the sputter silver coating does not act as a significant tissue
irritant.
AU - Fung, L. C. T.
AU - Khoury, A. E.
AU - Vas, S. I.
AU - Smith, C.
AU - Oreopoulos, D. G.
AU - Mittelman, M. W.
DB - Scopus
DO - 10.1177/089686089601600414
IS - 4
Catheter
Indwelling
Infections
Peritoneal dialysis
Silver
dacron
silicone
silver
animal experiment
animal model
animal tissue
article
bacterial colonization
biocompatibility
catheter infection
controlled study
giant cell
indwelling catheter
male
muscle
nonhuman
peritoneal dialysis
priority journal
subcutaneous fat
swine
time
M3 - Article
PY - 1996
SP - 398-405
ST - Biocompatibility of silver-coated peritoneal dialysis catheter in a porcine
model
T2 - Peritoneal Dialysis International
TI - Biocompatibility of silver-coated peritoneal dialysis catheter in a porcine
model
0029811654&doi=10.1177%2f089686089601600414&partnerID=40&md5=7f6a29497a614926ac54ad
3fabe41941
VL - 16
ID - 5817
ER -
TY - JOUR
AB - Background: Disruption of bile acid (BA) homeostasis plays a key role in
intestinal inflammation. The gut-liver axis is the main site for the regulation of
BA synthesis and BA pool size via the combined action of the nuclear Farnesoid X
Receptor (FXR) and the enterokine Fibroblast Growth Factor 19 (FGF19). Increasing
evidence have linked derangement of BA metabolism with dysbiosis and mucosal
inflammation. Thus, here we aimed to investigate the potential action of an FGF19
analogue on intestinal microbiota and inflammation. Methods: A novel engineered
non-tumorigenic variant of the FGF19 protein, M52-WO 2016/0168219 was generated. WT
and FXRnull mice were injected with AAV-FGF19-M52 or the control AAV-GFP and
subjected to Sodium Dextran Sulphate-induced colitis. Findings: FGF19-M52 reduced
BA synthesis and pool size, modulated its composition and protected mice from
intestinal inflammation. These events were coupled with preservation of the
intestinal epithelial barrier integrity, inhibition of inflammatory immune response
and modulation of microbiota composition. Interestingly, FGF19-M52-driven systemic
and local anti-inflammatory activity was completely abolished in Farnesoid X
Receptor (FXR)null mice, thus underscoring the need of FXR to guarantee
enterocytes’ fitness and complement FGF19 anti-inflammatory activity. To provide a
translational perspective, we also show that circulating FGF19 levels are reduced
in patients with Crohn's disease. Interpretation: Reactivation of the FXR-FGF19
axis in a murine model of intestinal inflammation could bona fide provide positive
changes in BA metabolism with consequent reduction of intestinal inflammation and
modulation of microbiota. These results point to the therapeutic potential of FGF19
in the treatment of intestinal inflammation with concomitant derangement of BA
homeostasis. Funding: A. Moschetta is funded by MIUR-PRIN 2017 <- 2017J3E2W2;
Italian Association for Cancer Research (AIRC, IG 23239); Interreg V-A Greece-Italy
2014-2020-SILVER WELLBEING, MIS5003627; HDHL-INTIMIC EuJPI-FATMAL; MIUR PON “R&I”
2014-2020-ARS01_01220. No money has been paid by NGM Biopharmaceuticals or any
other agency to write this article. © 2020 The Author(s)
AU - Gadaleta, R. M.
AU - Garcia-Irigoyen, O.
AU - Cariello, M.
AU - Scialpi, N.
AU - Peres, C.
AU - Vetrano, S.
AU - Fiorino, G.
AU - Danese, S.
AU - Ko, B.
AU - Luo, J.
AU - Porru, E.
AU - Roda, A.
AU - Sabbà, C.
AU - Moschetta, A.
C7 - 102719
DB - Scopus
DO - 10.1016/j.ebiom.2020.102719
KW - Bile acids
DSS-colitis
Enterokine
Intestinal inflammation
Nuclear receptors
Animals
Bile Acids and Salts
Colitis, Ulcerative
Crohn Disease
Female
Fibroblast Growth Factors
Humans
Male
Mice
Mice, Inbred C57BL
Peptides
Receptors, Cytoplasmic and Nuclear
Recombinant Proteins
adeno associated virus vector
bile acid
chenodeoxycholic acid
cholesterol 7alpha monooxygenase
cholic acid
claudin 2
dextran sulfate
farnesoid X receptor
fibroblast growth factor 19
interleukin 1beta
interleukin 6
kruppel like factor 4
messenger RNA
occludin
RNA 16S
transcription factor Cdx2
cell receptor
farnesoid X-activated receptor
fibroblast growth factor
fibroblast growth factor 15, mouse
peptide
recombinant protein
Anaeroplasmataceae
animal experiment
animal model
animal tissue
Article
body weight loss
colitis
controlled study
Crohn disease
cytotoxicity
dysbiosis
electrospray
gene expression
goblet cell
high throughput sequencing
histology
homeostasis
human
image analysis
inflammation
intestine flora
intestine mucosa permeability
male
metagenomics
Mollicutes
mouse
nonhuman
priority journal
rectum hemorrhage
remission
RNA extraction
ulcer
animal
C57BL mouse
chemistry
female
genetics
metabolism
microbiology
ulcerative colitis
M3 - Article
PY - 2020
ST - Fibroblast Growth Factor 19 modulates intestinal microbiota and inflammation
in presence of Farnesoid X Receptor
T2 - EBioMedicine
TI - Fibroblast Growth Factor 19 modulates intestinal microbiota and inflammation
in presence of Farnesoid X Receptor
85082820119&doi=10.1016%2fj.ebiom.2020.102719&partnerID=40&md5=2010285f7f4ba2bc1bfb
0c9946c71770
VL - 54
ID - 5335
ER -
TY - JOUR
AB - Because of their antimicrobial properties, the use of silver nanoparticles
(AgNPs) is increasing fast in industry, food, and medicine. In the food industry,
nanoparticles are used in packaging to enable better conservation products such as
sensors to track their lifetime, and as food additives, such as anti-caking agents
and clarifying agents for fruit juices. Nanoemulsions, used to encapsulate, protect
and deliver additives are also actively developed. Nanomaterials in foods will be
ingested and passed through the digestive tract. Those incorporated in food
packaging may also be released unintentionally into food, ending up in the
gastrointestinal tract. It is therefore important to make a risk assessment of
nanomaterials to the consumer. Thus, exposure to AgNPs is increasing in quantity
and it is imperative to know their adverse effects in man. However, controversies
still remain with respect to their toxic effects and their mechanisms.
Understanding the toxic effects and the interactions of AgNPs with biological
systems is necessary to handle these nanoparticles and their use. They usually
generate reactive oxygen species resulting in increased pro-inflammatory reactions
and oxidative stress via intracellular signalling pathways. Here, we mainly focus
on the routes of exposure of AgNPs, toxic effects and the mechanisms underlying the
induced toxicity. © 2014 Elsevier Ltd.
AU - Gaillet, S.
AU - Rouanet, J. M.
DB - Scopus
DO - 10.1016/j.fct.2014.12.019
KW - Mechanisms of action
Oral exposure
Oxidative stress
Toxicity
Animals
Food Additives
Food Contamination
Humans
Metal Nanoparticles
Oxidative Stress
Silver
silver nanoparticle
food additive
metal nanoparticle
silver
apoptosis
excretion
exposure
food industry
gastrointestinal absorption
human
inflammation
ingestion
intracellular signaling
nonhuman
oxidative stress
Review
risk assessment
tissue distribution
toxicity
toxicokinetics
analysis
animal
drug effects
food contamination
metabolism
oral drug administration
M3 - Review
PY - 2015
SP - 58-63
ST - Silver nanoparticles: Their potential toxic effects after oral exposure and
underlying mechanisms - A review
TI - Silver nanoparticles: Their potential toxic effects after oral exposure and
underlying mechanisms - A review
84921306344&doi=10.1016%2fj.fct.2014.12.019&partnerID=40&md5=1a1e9b461b5e87456d3e46
77f72fa305
VL - 77
ID - 5588
ER -
TY - JOUR
AB - With the increasing use and incorporation of nanoparticles (NPs) into
consumer products, screening for potential toxicity is necessary to ensure customer
safety. NPs have been shown to translocate to the bloodstream following inhalation
and ingestion, and such studies demonstrate that the liver is an important organ
for accumulation. Silver (Ag) NPs are highly relevant for human exposure due to
their use in food contact materials, dietary supplements, and antibacterial wound
treatments. Due to the large number of different NPs already used in various
products and being developed for new applications, it is essential that relevant,
quick, and cheap methods of in vitro risk assessment suitable for these new
materials are established. Therefore, this study used a simple hepatocytes model
combined with an in vivo injection model to simulate the passage of a small amount
of NPs into the bloodstream following exposure, e.g., via ingestion or inhalation,
and examined the potential of Ag NPs of 20nm diameter to cause toxicity,
inflammation, and oxidative stress in the liver following in vivo exposures of
female Wistar rats via iv injection to 50 μg of NPs and in vitro exposures using
the human hepatocyte cell line C3A. We found that Ag NPs were highly cytotoxic to
hepatocytes (LC50 lactate dehydrogenase: 2.5 μg/cm2) and affected hepatocyte
homeostasis by reducing albumin release. At sublethal concentrations with normal
cell or tissue morphology, Ag NPs were detected in cytoplasm and nuclei of
hepatocytes. We observed similar effects of Ag NPs on inflammatory mediator
expression in vitro and in vivo with increase of interleukin-8 (IL-8)/macrophage
inflammatory protein 2, IL-1RI, and tumor necrosis factor-α expression in both
models and increased IL-8 protein release in vitro. This article presents evidence
of the potential toxicity and inflammogenic potential of Ag NPs in the liver
following ingestion. In addition, the similarities between in vitro and in vivo
responses are striking and encouraging for future reduction, refinement, and
replacement of animal studies by the use of hepatocyte cell lines in particle risk
assessment. © The Author 2012. Published by Oxford University Press on behalf of
the Society of Toxicology. All rights reserved.
AU - Gaiser, B. K.
AU - Hirn, S.
AU - Kermanizadeh, A.
AU - Kanase, N.
AU - Fytianos, K.
AU - Wenk, A.
AU - Haberl, N.
AU - Brunelli, A.
AU - Stone, V.
DB - Scopus
DO - 10.1093/toxsci/kfs306
IS - 2
KW - Hepatocytes
In vitro alternatives
Liver toxicology
Metals
Nanoparticles
Animals
Apoptosis
Cell Line
Female
Flow Cytometry
Glutathione
Liver
Metal Nanoparticles
Microscopy, Confocal
Rats
Rats, Wistar
RNA, Messenger
Silver
albumin
interleukin 1 receptor type I
interleukin 8
silver nanoparticle
animal experiment
article
blood flow
cell line
cell nucleus
cell structure
controlled study
cytokine production
cytoplasm
cytotoxicity
female
homeostasis
human
human cell
in vitro study
in vivo study
ingestion
injection
liver
liver cell
liver toxicity
low drug dose
mediator
nonhuman
protein expression
protein secretion
rat
M3 - Article
PY - 2013
SP - 537-547
ST - Effects of silver nanoparticles on the liver and hepatocytes in vitro
T2 - Toxicological Sciences
TI - Effects of silver nanoparticles on the liver and hepatocytes in vitro
84873857913&doi=10.1093%2ftoxsci
%2fkfs306&partnerID=40&md5=2f4a86345fa373059b4456f02ce75e51
VL - 131
ID - 5682
ER -
TY - JOUR
AB - Biomedical application of silver nanoparticles (AgNPs) has been rapidly
increasing. Owing to their strong antimicrobial activity, AgNPs are used in
dermatology in the treatment of wounds and burns. However, recent evidence for
their cytotoxicity gives rise to safety concerns. This study was undertaken as a
part of an ongoing programme in our laboratory to develop a topical agent for wound
healing. Here, we investigated the potential toxicity of AgNPs using normal human
dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK) with the
aim of comparing the effects of AgNPs and ionic silver (Ag-I). Besides the effect
of AgNPs and Ag-I on cell viability, the inflammatory response and DNA damage in
AgNPs and Ag-I-treated cells were examined. The results showed that Ag-I were
significantly more toxic than AgNPs both on NHDF and NHEK. Non-cytotoxic
concentrations of AgNPs and Ag-I did not induce DNA strand breaks and did not
affect inflammatory markers, except for a transient increase in interleukin 6
levels in Ag-I-treated NHDF. The results showed that AgNPs are more suitable for
the intended application as a topical agent for wound healing up to the
concentration 25 μg/mL. © SAGE Publications.
AU - Galandáková, A.
AU - Franková, J.
AU - Ambrožová, N.
AU - Habartová, K.
AU - Pivodová, V.
AU - Zálešák, B.
AU - Šafářová, K.
AU - Smékalová, M.
AU - Ulrichová, J.
DB - Scopus
DO - 10.1177/0960327115611969
IS - 9
KW - DNA damage
Human dermal fibroblasts
human epidermal keratinocytes
inflammation
toxicity
Cell Survival
Cells, Cultured
DNA Damage
Epidermis
Fibroblasts
Humans
Keratinocytes
Metal Nanoparticles
Silver
Skin
Surface Properties
biological marker
cyclooxygenase 2
interleukin 6
interleukin 8
silver nanoparticle
silver nitrate
antiinfective agent
metal nanoparticle
silver
Article
cell viability
comparative study
controlled study
cytotoxicity
dermatitis
DNA strand breakage
epidermis
genotoxicity
human
human cell
keratinocyte
nanotoxicology
particle size
priority journal
protein expression
skin fibroblast
skin toxicity
zeta potential
cell culture
cell culture technique
cell survival
chemistry
dose response
drug effects
fibroblast
pathology
skin
surface property
M3 - Article
PY - 2016
SP - 946-957
ST - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
T2 - Human and Experimental Toxicology
TI - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
84979673390&doi=10.1177%2f0960327115611969&partnerID=40&md5=ac76a3543051032617a912a
715ea4b75
VL - 35
ID - 5474
ER -
TY - JOUR
AB - Biomedical application of silver nanoparticles (AgNPs) has been rapidly
increasing. Owing to their strong antimicrobial activity, AgNPs are used in
dermatology in the treatment of wounds and burns. However, recent evidence for
their cytotoxicity gives rise to safety concerns. This study was undertaken as a
part of an ongoing programme in our laboratory to develop a topical agent for wound
healing. Here, we investigated the potential toxicity of AgNPs using normal human
dermal fibroblasts (NHDF) and normal human epidermal keratinocytes (NHEK) with the
aim of comparing the effects of AgNPs and ionic silver (Ag-I). Besides the effect
of AgNPs and Ag-I on cell viability, the inflammatory response and DNA damage in
AgNPs and Ag-l treated cells were examined. The results showed that Ag-I were
significantly more toxic than AgNPs both on NHDF and NHEK. Noncytotoxic
concentrations of AgNPs and Ag-I did not induce DNA strand breaks and did not
affect inflammatory markers, except for a transient increase in interleukin 6
levels in Ag-I treated NHDF. The results showed that AgNPs are more suitable for
the intended application as a topical agent for wound healing up to the
concentration 25 mu g/mL.
AN - WOS:000381951900005
AU - Galandakova, A.
AU - Frankova, J.
AU - Ambrozova, N.
AU - Habartova, K.
AU - Pivodova, V.
AU - Zalesak, B.
AU - Safarova, K.
AU - Smekalova, M.
AU - Ulrichova, J.
DA - SEP
DO - 10.1177/0960327115611969
IS - 9
PY - 2016
SN - 0960-3271
1477-0903
SP - 946-957
ST - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
T2 - HUMAN & EXPERIMENTAL TOXICOLOGY
TI - Effects of silver nanoparticles on human dermal fibroblasts and epidermal
keratinocytes
VL - 35
ID - 5904
ER -
TY - JOUR
AB - This study evaluated the biodistribution and organ oxidative effects of
silver nanoparticles (AgNPs) coated with/without polyvinylpyrrolidone (PVP) (AgNP-
20 and AgNP-PVP) in mice; these were administered by gavage at a dose of 10-250
mg/kg body weight per day for 28 days. The results showed that both the AgNPs could
induce subacute toxicity and oxidative damage to mice and were mainly accumulated
in the liver and spleen and excreted by feces. AgNPs could be absorbed into blood
and might cross the blood-brain barrier, and be distributed extensively in mice.
The malondialdehyde content in the liver, lungs and kidneys increased in both AgNP
groups, while the content of glutathione decreased, and the activity of superoxide
dismutase increased at first and then decreased along with the increased doses.
Inflammatory pathological changes in the lung and liver at high dose of both AgNPs
were consistent with increases in glutamate pyruvic transaminase, glutamate
oxaloacetic transaminase and the total protein in serum detection. The Ag content
was detected in organs, with the highest content in the liver, followed by spleen,
while the Ag content in feces was about 500 times higher than that in urine. AgNP-
PVP could induce higher oxidative stress and subacute toxicity than AgNP-20 at the
same dose, which might be related to the higher concentrations and more Ag+ ions
released in mice after AgNP-PVP exposure. The data from this research provided
information on toxicity and biodistribution of AgNPs following gavage
administration in mice, and might shed light for future application of AgNPs in
daily life. © 2020 John Wiley & Sons, Ltd.
AU - Gan, J.
AU - Sun, J.
AU - Chang, X.
AU - Li, W.
AU - Li, J.
AU - Niu, S.
AU - Kong, L.
AU - Zhang, T.
AU - Wu, T.
AU - Tang, M.
AU - Xue, Y.
DB - Scopus
DO - 10.1002/jat.3946
IS - 6
KW - distribution
excretion
oxidative stress
subacute toxicity
Animals
Female
Male
Metal Nanoparticles
Mice, Inbred ICR
Povidone
Silver Compounds
Tissue Distribution
glutathione
hydroxypropylmethylcellulose
malonaldehyde
povidone
silver nanoparticle
metal nanoparticle
silver derivative
animal experiment
animal model
animal tissue
Article
body weight gain
brain tissue
cell infiltration
coating (procedure)
controlled study
female
heart tissue
kidney tissue
liver cell
liver tissue
lung parenchyma
male
mouse
nonhuman
particle size
priority journal
spleen tissue
zeta potential
animal
metabolism
tissue distribution
M3 - Article
PY - 2020
SP - 815-831
ST - Biodistribution and organ oxidative damage following 28 days oral
administration of nanosilver with/without coating in mice
TI - Biodistribution and organ oxidative damage following 28 days oral
administration of nanosilver with/without coating in mice
85078751051&doi=10.1002%2fjat.3946&partnerID=40&md5=c3daf58018af98f3cb24170cadb460a
0
VL - 40
ID - 5292
ER -
TY - JOUR
AB - Chronic wounds affect healthcare systems and increase the risk of infection.
Despite the clinical demand for improving healing, an effective and safe
therapeutic approach is still lacking. Here, we present a novel spatio-temporal-
varying adhesive microneedle (MN) patch encapsulated with mesenchymal stem cell-
derived exosomes (MSC-exos) and antibacterial Ag nanoparticles (AgNPs) for
improving wound healing. This MN patch is fabricated by replicating a predesigned
flexible template. Consisting of porous methacrylate gelatin hydrogels, the MN tips
can continuously deliver anti-inflammatory and pro-angiogenic MSC-exos to wound
beds, which can accelerate healing by improving cell functions, remodeling blood
vessels and restoring immune systems. Besides, the silk-fibroin-composed MN back
enables the patch to stick to the skin well and fix at the wounded site, thus
avoiding secondary bandage. By incorporating AgNPs in the MN back, the MN patch is
endowed with the ability to efficiently resist microbial contamination and further
benefits wound repair. Such a composite MN patch performs well in relieving
inflammation, promoting angiogenesis, and suppressing wound-infecting bacteria
during wound injury in diabetic rats, with no adverse effects observed. All the
features make this multifunctional MN patch potentially valuable for clinical
applications. © 2022
AU - Gan, J.
AU - Zhang, X.
AU - Ma, W.
AU - Zhao, Y.
AU - Sun, L.
C7 - 101630
DB - Scopus
DO - 10.1016/j.nantod.2022.101630
KW - Bioinspired
Microneedle
MSC-exosome
Patch
Wound healing
Adhesives
Blood vessels
Cell culture
Needles
Stem cells
adhesive agent
CD11b antigen
CD63 antigen
CD81 antigen
CD86 antigen
CD9 antigen
dimeticone
gelatin
hydrogel
interleukin 10
interleukin 6
methacrylic acid
silk fibroin
silver nanoparticle
vasculotropin
vasculotropin A
Antibacterials
Exosomes
Microneedle patches
Microneedles
Spatio-temporal variation
agar diffusion
angiogenesis
animal cell
animal experiment
animal model
Article
biocompatibility
blood vessel
controlled study
diabetic wound
drug cytotoxicity
elasticity
exosome
human
human cell
immunofluorescence
in vitro study
macrophage
male
microbial contamination
NIH 3T3 cell line
nonhuman
permeability
rat
RAW 264.7 cell line
Western blotting
wound healing
M3 - Article
PY - 2022
ST - Antibacterial, adhesive, and MSC exosomes encapsulated microneedles with
spatio-temporal variation functions for diabetic wound healing
T2 - Nano Today
TI - Antibacterial, adhesive, and MSC exosomes encapsulated microneedles with
spatio-temporal variation functions for diabetic wound healing
85139297839&doi=10.1016%2fj.nantod.2022.101630&partnerID=40&md5=d76ffaccc4a44b14f26
6db520be79a82
VL - 47
ID - 5002
ER -
TY - JOUR
AB - The Lichen, Parmelia sulcata synthesizes various secondary metabolites, in
which phenolic based compounds received much attention due to their importance in
biomedical application. Especially the phenolic compound was effective against the
cancer treatment. An effective administration of such plant natural product can
represent a significant conventional management of cancer in terms of
chemoprevention. The nanomedicines are group of agents that selectively interfere
the cancer cells which leads to reduction of side effect thereby reducing the
doses. Silver nanoparticles is a promising antitumor agent, however, the
conventional production of silver nanoparticles have many drawbacks which led to
increase in need of eco-friendly biological production methods. In this study, we
made an attempt to synthesise a nano silver (Ps-AgNPs) from phenolic extract of
lichen Parmelia sulcata extract. The Ps-AgNps was applied for anticancer activity
using MCF-7 cells and the effect was characterised by western blotting method. The
FTIR, XRD, UV and TEM results confirms the presence of silver nanoparticles in
phenolic extract of lichen Parmelia sulcata. The cytotoxicity assay shows that the
Ps-AgNPs is toxic against cancer cells (MCF-7) but not to normal cells (NIH3T3),
which confirm the selective induction of cell death (apoptosis) against cancer
cells. The Western blot analysis also clearly indicates the down regulation of
inflammatory genes (TNF-alpha and IL-6) and cell cycle genes (PCNA and Cyclin-D1)
thus promoting intrinsic apoptotic pathway. The results suggest that Ps-AgNPs can
effectively kill cancer cells and can be used as an alternative therapeutic agent
for cancer treatment. © 2021 Elsevier Inc.
AU - Gandhi, A. D.
AU - Miraclin, P. A.
AU - Abilash, D.
AU - Sathiyaraj, S.
AU - Velmurugan, R.
AU - Zhang, Y.
AU - Soontarapa, K.
AU - Sen, P.
AU - Sridharan, T. B.
C7 - 111375
DB - Scopus
DO - 10.1016/j.envres.2021.111375
KW - Anticancer activity
Apoptosis
Parmelia sulcata
Phenolic compounds
Animals
Humans
MCF-7 Cells
Metal Nanoparticles
Mice
NIH 3T3 Cells
Parmeliaceae
Plant Extracts
Silver
antimitotic agent
apoptosis inducing factor
cyclin D1
cycline
cytotoxic agent
doxorubicin
interleukin 6
Parmelia sulcata extract
plant extract
silver nanoparticle
unclassified drug
antiinfective agent
metal nanoparticle
silver
apoptosis
biological production
lichen
nanoparticle
phenolic compound
Article
cell death
controlled study
down regulation
drug cytotoxicity
lichen (organism)
MCF-7 cell line
nanomedicine
proapoptotic activity
signal transduction
upregulation
Western blotting
X ray diffraction
animal
human
mouse
NIH 3T3 cell line
M3 - Article
PY - 2021
ST - Nanosilver reinforced Parmelia sulcata extract efficiently induces apoptosis
and inhibits proliferative signalling in MCF-7 cells
TI - Nanosilver reinforced Parmelia sulcata extract efficiently induces apoptosis
and inhibits proliferative signalling in MCF-7 cells
85108123711&doi=10.1016%2fj.envres.2021.111375&partnerID=40&md5=83a4dccf562180f34cc
03d6231a9ac6d
VL - 199
ID - 5191
ER -
TY - JOUR
AB - Nanoparticle preparations of heavy metals have attracted enormous scientific
and technological interest. Biologically produced nanoparticle preparations of
heavy metals are elaborately described in traditional texts and being widely
prescribed. The underlying interactions of nano preparations within the
physiological fluids are key feature to understand their biological impact. In this
perspective, we performed an experimental assessment of the toxicity potential of a
marketed metallic preparation named Vasant Kusumakar Ras (VKR), wherein different
heavy metals in composite form are reduced to nanoparticle size to produce the
desired effect in diabetes and its complications. VKR (50. mg/kg) was administered
to Albino Wistar rats rendered diabetic using streptozotocin (90. mg/kg) in 2 days
old neonates. Anti-hyperglycemic effect was observed with VKR along with increased
levels of plasma insulin. Renal variables including total proteins and albumin
along with glomerular filtration rate were found to improve biochemically. The
results were supplemented by effects on different inflammatory and growth factors
like TNF-α, nitric oxide, TGF-β and VEGF. However, the results observed in kidney
histopathology were not in accordance with the biochemical parameters. Inflammation
observed in kidney was confirmed by immunostaining metallothionein, which was due
to the accumulation of heavy metals. Furthermore, mercury accumulation in kidney
further confirmed by autometallography, which activated mononuclear phagocyte
system, which generated an immune response. This was further supported by increase
in the extent of apoptosis in kidney tissues. In conclusion, nanoparticle
preparations of heavy metals can be toxic to kidney if it is not regulated with
respect to its surface chemistry and dosage. © 2013 Elsevier GmbH.
AU - Gandhi, S.
AU - Srinivasan, B. P.
AU - Akarte, A. S.
DB - Scopus
DO - 10.1016/j.etp.2013.05.004
IS - 7-8
KW - Apoptosis
Autometallography
Diabetic nephropathy
Mercury
Metallothionein
Nephrin
Animals
DNA Fragmentation
Glomerular Filtration Rate
Insulin
Kidney
Medicine, Ayurvedic
Metal Nanoparticles
Metals, Heavy
Rats
Rats, Wistar
Rattus norvegicus
adiponectin
albumin
creatinine
cystatin C
erythropoietin
glucose transporter 2
glucose transporter 4
gold
heavy metal
insulin
iron
lead
mercury
nanoparticle
nephrin
nitric oxide
silver
tin
vasculotropin
albumin blood level
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
controlled study
diabetic nephropathy
experimental study
glomerulus filtration rate
histopathology
immune response
insulin blood level
kidney parenchyma
non insulin dependent diabetes mellitus
nonhuman
particle size
protein blood level
protein expression
rat
streptozocin diabetes
toxicity testing
M3 - Article
PY - 2013
SP - 1127-1135
ST - An experimental assessment of toxic potential of nanoparticle preparation of
heavy metals in streptozotocin induced diabetes
T2 - Experimental and Toxicologic Pathology
TI - An experimental assessment of toxic potential of nanoparticle preparation of
heavy metals in streptozotocin induced diabetes
84885855265&doi=10.1016%2fj.etp.2013.05.004&partnerID=40&md5=a356d49f243d3d96075952
f81d3e7ad2
VL - 65
ID - 5646
ER -
TY - JOUR
AB - Urinary tract infections (UTIs) caused by the contamination of the ureteral
stent and the pain associated with secondary stent extractions are worldwide
problems in the treatment of urinary tract disorders. Here, we reported a
biodegradable, long-term antibacterial, and extraction-free ureteral stent with a
constantly renewable contact-killing surface and an antibiofilm function achieved
by constructing a hyperbranched poly(amide-amine)-capped Ag shell and Au core
nanoparticle (Ag@Au NP)-embedded fiber membrane-structured poly(glycolic
acid)/poly(lactic-co-glycolic acid) (PGA/PGLA) ureteral stent. The ureteral stent
showed fast contact-killing properties, i.e., 5 min for Escherichia coli and 10 min
for Staphylococcus aureus, with an inhibition rate higher than 99%. In addition,
gradient degradation of PGA/PGLA endowed the stent with a self-cleaning property
and long-term antibacterial function by continuous exfoliation of the stent
surface, thereby exposing the inner Ag@Au NPs and eliminating adherent bacteria and
proteins. Subsequently, in the 16-day in vitro degradation test, the stent showed
durable bactericidal activity, less total release of Ag and Au elements (6.7%,
similar to 8 mu g), and low cytotoxicity (with a relative growth rate of >80% of
L929 cells). In vivo experiments on a farm pig model showed that the stent
exhibited a remarkable antibiofilm property and reduced the level of inflammatory
and necrotic cells. After seven days of implantation, the stent showed a gradient
degradation behavior and maintained structural integrity without the presence of
any large fragments in the urinary system according to the B-ultrasonic
examination. The as-developed biodegradable and renewable contact-killing
antibacterial strategy was efficient in preparing the ureteral stent with
antibiofilm and extraction-free properties to treat stent-induced UTI. Statement of
significance This study presents a customized antibiofilm solution for
biodegradable implants. Two particularly important aspects of this work are as
follows: (1) The ureteral stent showed functions of bacterial inactivation and
removal of dead bacterial debris. It can continuously exfoliate the stent surface,
expose internal antibacterial agents, and remove adherent bacteria and proteins.
The stent exhibited a remarkable antibiofilm property and reduced the level of
inflammatory and necrotic cells after three weeks of implantation. (2) The
antibacterial agents were firmly attached to the degrading matrix by hydrogen
bonding, thereby resulting in long-term bactericidal activity, low total release of
the antibacterial agent (6.7%, similar to 8 mu g), and low cytotoxicity (with a
relative growth rate of >80% of L929 cells). (C) 2020 Acta Materialia Inc.
Published by Elsevier Ltd. All rights reserved.
AN - WOS:000567880100009
AU - Gao, L. H.
AU - Wang, Y. W.
AU - Li, Y. M.
AU - Xu, M. X.
AU - Sun, G.
AU - Zou, T.
AU - Wang, F. J.
AU - Xu, S. J.
AU - Da, J.
AU - Wang, L.
DA - SEP 15
DO - 10.1016/j.actbio.2020.07.025
PY - 2020
SN - 1742-7061
1878-7568
SP - 117-132
ST - Biomimetic biodegradable Ag@Au nanoparticle-emb edded ureteral stent with a
constantly renewable contact-killing antimicrobial surface and antibiofilm and
extraction-free properties
TI - Biomimetic biodegradable Ag@Au nanoparticle-emb edded ureteral stent with a
VL - 114
ID - 6809
ER -
TY - JOUR
AB - In the present study genipin crosslinked chitosan (CHI) hydrogels, which had
been constructed and reported in our previous studies (Gao et al., 2014 [22]), were
further evaluated for their advantage as a carrier for silver sulfadiazine (AgSD)
nanocrystal systems. Firstly, AgSD nanocrystals with a mean particle size of 289 nm
were prepared by wet milling method and encapsulated into genipin crosslinked CHI
hydrogels. AgSD nanocrystals displayed a uniform distribution and very good
physical stability in the hydrogel network. Swelling-dependent release pattern was
found for AgSD nanocrystals from hydrogels and the release profile could be well
fitted with Peppas equation. When AgSD nanocrystals were encapsulated in hydrogels
their fibroblast cytotoxicity decreased markedly, and their antibacterial effects
against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were
still comparable to unencapsulated AgSD nanocrystals. In vivo evaluation in
excision and burn cutaneous wound models in mice showed that AgSD nanocrystal
hydrogels markedly decreased the expression of inflammatory cytokine IL-6, but
increased the levels of growth factors VEGF-A and TGF-beta 1. Histopathologically,
the wounds treated by hydrogels containing AgSD nanocrystals showed the best
healing state compared with commercial AgSD cream, hydrogels containing AgSD bulk
powders and blank hydrogels. The wounds treated by AgSD nanocrystal hydrogels were
dominated by marked fibroblast proliferation, new blood vessels and thick
regenerated epithelial layer. Sirius Red staining assay indicated that AgSD
nanocrystal hydrogels resulted in more collagen deposition characterized by a large
proportion of type I fibers. Our study suggested that genipin-crosslinked CHI
hydrogel was a potential carrier for local antibacterial nanomedicines. (C) 2016
Published by Elsevier B.V.
AN - WOS:000388248500039
AU - Gao, L.
AU - Gan, H.
AU - Meng, Z. Y.
AU - Gu, R. L.
AU - Wu, Z. N.
AU - Zhu, X. X.
AU - Sun, W. Z.
AU - Li, J.
AU - Zheng, Y.
AU - Sun, T.
AU - Dou, G. F.
DA - DEC 1
DO - 10.1016/j.colsurfb.2016.06.016
PY - 2016
SN - 0927-7765
1873-4367
SP - 343-353
ST - Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier
for silver sulfadiazine nanocrystals
TI - Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier
VL - 148
ID - 6456
ER -
TY - JOUR
AB - In the present study genipin crosslinked chitosan (CHI) hydrogels, which had
been constructed and reported in our previous studies (Gao et al., 2014 [22]), were
further evaluated for their advantage as a carrier for silver sulfadiazine (AgSD)
nanocrystal systems. Firstly, AgSD nanocrystals with a mean particle size of 289 nm
were prepared by wet milling method and encapsulated into genipin crosslinked CHI
hydrogels. AgSD nanocrystals displayed a uniform distribution and very good
physical stability in the hydrogel network. Swelling-dependent release pattern was
found for AgSD nanocrystals from hydrogels and the release profile could be well
fitted with Peppas equation. When AgSD nanocrystals were encapsulated in hydrogels
their fibroblast cytotoxicity decreased markedly, and their antibacterial effects
against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were
still comparable to unencapsulated AgSD nanocrystals. In vivo evaluation in
excision and burn cutaneous wound models in mice showed that AgSD nanocrystal
hydrogels markedly decreased the expression of inflammatory cytokine IL-6, but
increased the levels of growth factors VEGF-A and TGF-β1. Histopathologically, the
wounds treated by hydrogels containing AgSD nanocrystals showed the best healing
state compared with commercial AgSD cream, hydrogels containing AgSD bulk powders
and blank hydrogels. The wounds treated by AgSD nanocrystal hydrogels were
dominated by marked fibroblast proliferation, new blood vessels and thick
regenerated epithelial layer. Sirius Red staining assay indicated that AgSD
nanocrystal hydrogels resulted in more collagen deposition characterized by a large
proportion of type I fibers. Our study suggested that genipin-crosslinked CHI
hydrogel was a potential carrier for local antibacterial nanomedicines. © 2016
AU - Gao, L.
AU - Gan, H.
AU - Meng, Z.
AU - Gu, R.
AU - Wu, Z.
AU - Zhu, X.
AU - Sun, W.
AU - Li, J.
AU - Zheng, Y.
AU - Sun, T.
AU - Dou, G.
DB - Scopus
DO - 10.1016/j.colsurfb.2016.06.016
KW - Antibacterial
Chitosan
Hydrogel
Silver sulfadiazine
Wound healing
Drug Carriers
Hydrogels
Iridoids
Nanoparticles
Silver Sulfadiazine
Bacteria
Biomaterials
Blood vessels
Cell culture
Chitin
Escherichia coli
Fibroblasts
Nanocrystals
Particle size
Silver
genipin
interleukin 6
sulfadiazine silver
vasculotropin A
chitosan
drug carrier
hydrogel
iridoid
nanoparticle
Antibacterial effects
Cross linked chitosan
Fibroblast proliferation
Silver sulfadiazines
Article
biocompatibility
cell proliferation
controlled study
cross linking
cytotoxicity
drug release
drug solubility
drug stability
encapsulation
fibroblast
histology
in vitro study
nonhuman
particle size
powder
priority journal
protein expression
skin injury
wound contraction
chemistry
M3 - Article
PY - 2016
SP - 343-353
ST - Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier
TI - Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier
84986552418&doi=10.1016%2fj.colsurfb.2016.06.016&partnerID=40&md5=be2a0264e48825640
6e40c3f62624525
VL - 148
ID - 5506
ER -
TY - JOUR
AB - Urinary tract infections (UTIs) caused by the contamination of the ureteral
stent and the pain associated with secondary stent extractions are worldwide
problems in the treatment of urinary tract disorders. Here, we reported a
biodegradable, long-term antibacterial, and extraction-free ureteral stent with a
constantly renewable contact-killing surface and an antibiofilm function achieved
by constructing a hyperbranched poly(amide-amine)-capped Ag shell and Au core
nanoparticle (Ag@Au NP)-embedded fiber membrane-structured poly(glycolic
acid)/poly(lactic-co-glycolic acid) (PGA/PGLA) ureteral stent. The ureteral stent
showed fast contact-killing properties, i.e., 5 min for Escherichia coli and 10 min
for Staphylococcus aureus, with an inhibition rate higher than 99%. In addition,
gradient degradation of PGA/PGLA endowed the stent with a self-cleaning property
and long-term antibacterial function by continuous exfoliation of the stent
surface, thereby exposing the inner Ag@Au NPs and eliminating adherent bacteria and
proteins. Subsequently, in the 16-day in vitro degradation test, the stent showed
durable bactericidal activity, less total release of Ag and Au elements (6.7%, ~8
μg), and low cytotoxicity (with a relative growth rate of >80% of L929 cells). In
vivo experiments on a farm pig model showed that the stent exhibited a remarkable
antibiofilm property and reduced the level of inflammatory and necrotic cells.
After seven days of implantation, the stent showed a gradient degradation behavior
and maintained structural integrity without the presence of any large fragments in
the urinary system according to the B-ultrasonic examination. The as-developed
biodegradable and renewable contact-killing antibacterial strategy was efficient in
preparing the ureteral stent with antibiofilm and extraction-free properties to
treat stent-induced UTI. Statement of significance This study presents a customized
antibiofilm solution for biodegradable implants. Two particularly important aspects
of this work are as follows: (1) The ureteral stent showed functions of bacterial
inactivation and removal of dead bacterial debris. It can continuously exfoliate
the stent surface, expose internal antibacterial agents, and remove adherent
bacteria and proteins. The stent exhibited a remarkable antibiofilm property and
reduced the level of inflammatory and necrotic cells after three weeks of
implantation. (2) The antibacterial agents were firmly attached to the degrading
matrix by hydrogen bonding, thereby resulting in long-term bactericidal activity,
low total release of the antibacterial agent (6.7%, ~8 μg), and low cytotoxicity
(with a relative growth rate of >80% of L929 cells). © 2020 Acta Materialia Inc.
AU - Gao, L.
AU - Wang, Y.
AU - Li, Y.
AU - Xu, M.
AU - Sun, G.
AU - Zou, T.
AU - Wang, F.
AU - Xu, S.
AU - Da, J.
AU - Wang, L.
DB - Scopus
DO - 10.1016/j.actbio.2020.07.025
KW - Antibiofilm
Contact killing
Gradient degradation
Ureteral stent
UTI
Animals
Biofilms
Biomimetics
Gold
Homicide
Metal Nanoparticles
Silver
Stents
Swine
Amides
Biodegradation
Extraction
Growth rate
Mammals
Morphology
Proteins
biomimetic material
gold nanoparticle
polyglactin
silver
silver nanoparticle
antiinfective agent
gold
metal nanoparticle
Antibacterials
Antibiofilms
Free property
Poly lactic-co-glycolic acid
Poly(glycolic acid)
Property
Urinary tract infections
antibiofilm activity
Article
biocidal activity
controlled study
cytotoxicity
degradation
echography
Escherichia coli
growth rate
nonhuman
priority journal
animal
biofilm
biomimetics
homicide
pig
stent
M3 - Article
PY - 2020
SP - 117-132
ST - Biomimetic biodegradable Ag@Au nanoparticle-embedded ureteral stent with a
TI - Biomimetic biodegradable Ag@Au nanoparticle-embedded ureteral stent with a
85088303358&doi=10.1016%2fj.actbio.2020.07.025&partnerID=40&md5=1ca8ba3a2314f1018aa
ddafe4ea46ac2
VL - 114
ID - 5320
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been increasingly used in a variety of
consumer products over the last decades. However, their potential adverse effects
have not been fully understood. In a previous study, we characterized
transcriptomic changes in human induced pluripotent stem cell (iPSC)-derived
hepatocyte-like cells (HLCs) in response to AgNP exposure. Here, we report findings
of a follow-up proteomic study that evaluated alternations at the protein level in
the same cell after being exposed to 10 mu g/ml AgNPs for 24 h. In total, 6287
proteins were identified across two groups of samples (n = 3). Among these
proteins, 665 were found to be differentially regulated (fold change >= 1.25, p <
0.01) between the AgNP-treated group and the untreated control group, including 264
upregulated and 401 downregulated. Bioinformatics analysis of the proteomics data,
in side-by-side comparison to the transcriptomics data, confirms and substantiates
previous findings on AgNP-induced alterations in metabolism, oxidative stress,
inflammation, and potential association with cancer. A mechanism of action was
proposed based on these results. Collectively, the findings of the current
proteomic study are consistent with those of the previous transcriptomic study and
further demonstrate the usefulness of iPSC-derived HLCs as an in vitro model for
liver nanotoxicology.
AN - WOS:000747784000007
AU - Gao, X. G.
AU - Li, R.
AU - Yourick, J. J.
AU - Sprando, R. L.
C6 - NOV 2021
C7 - 105274
DA - MAR
DO - 10.1016/j.tiv.2021.105274
PY - 2022
SN - 0887-2333
1879-3177
ST - Transcriptomic and proteomic responses of silver nanoparticles in hepatocyte-
like cells derived from human induced pluripotent stem cells
TI - Transcriptomic and proteomic responses of silver nanoparticles in hepatocyte-
like cells derived from human induced pluripotent stem cells
VL - 79
ID - 6094
ER -
TY - JOUR
AB - Background: Sulfur mustard (SM) inhalation causes apoptosis and death of
airway epithelial cells as well as inflammation in the airway. Efficient clearance
of the cell debris by alveolar macrophages is necessitated to reduce the
inflammation. Macrolide antibiotics have been reported to have anti-inflammatory
properties by modulating the production of proinflammatory cytokines and mediators,
and by improving macrophage functions. The present study investigated the effects
of four commonly used macrolide antibiotics, namely azithromycin, clarithromycin,
erythromycin, and roxithromycin, on chemotactic and phagocytotic function and on
inflammatory cytokines/mediators production in vitro in SM-exposed monocyte THP-1
cells. Results: Chemotaxis and phagocytosis of the monocytes reduced upon exposure
to 10 mu M SM (8.1% and 17.5%, respectively) were restored by treatment with 10 mu
M of any of the four macrolides. Overexpression of inflammatory cytokines following
SM exposure was decreased by 50-70% with macrolide treatment. Similarly,
exaggerated iNOS expression and nitric oxide (NO) production induced by SM exposure
was largely inhibited by treatment with macrolides. Conclusion: The data
demonstrate that macrolide antibiotics were effective in improving the degenerated
chemotactic and phagocytotic functions of monocytes following SM exposure, and in
reducing SM-induced overproduction of proinflammatory cytokines and mediators.
Thus, treatment with macrolide antibiotics may lead to improved clearance of
apoptotic material in the airway and ultimately result in reduced airway
inflammation and injury caused by SM inhalation, suggesting that macrolide
antibiotics may serve as potential vesicant respiratory therapeutics. Published by
Elsevier Ltd.
AN - WOS:000275356700005
AU - Gao, X. G.
AU - Ray, R.
AU - Xiao, Y.
AU - Ishida, K.
AU - Ray, P.
DA - APR
DO - 10.1016/j.pupt.2009.10.010
IS - 2
PY - 2010
SN - 1094-5539
SP - 97-106
ST - Macrolide antibiotics improve chemotactic and phagocytic capacity as well as
reduce inflammation in sulfur mustard-exposed monocytes
T2 - PULMONARY PHARMACOLOGY & THERAPEUTICS
TI - Macrolide antibiotics improve chemotactic and phagocytic capacity as well as
reduce inflammation in sulfur mustard-exposed monocytes
VL - 23
ID - 6629
ER -
TY - JOUR
AB - Background: Sulfur mustard (SM) is a potent chemical vesicant warfare agent
that remains a significant military and civilian threat. Inhalation of SM gas
causes airway inflammation and injury. In recent years, there has been increasing
evidence of the effectiveness of macrolide antibiotics in treating chronic airway
inflammatory diseases. In this study, the anti-cytotoxic and anti-inflammatory
effects of a representative macrolide antibiotic, roxithromycin, were tested in
vitro using SM-exposed normal human small airway epithelial (SAE) cells and
bronchial/tracheal epithelial (BTE) cells. Cell viability, expression of
proinflammatory cytokines including interleukin (IL)-1 beta, IL-6, IL-8 and tumor
necrosis factor (TNF), and expression of inducible nitric oxide synthase (iNOS)
were examined, since these proinflammatory cytokines/mediators are import
indicators of tissue inflammatory responses. We suggest that the influence of
roxithromycin on SM-induced inflammatory reaction could play an important
therapeutic role in the cytotoxicity exerted by this toxicant. Results: MTS assay
and Calcein AM/ethidium homodimer (EthD-1) fluorescence staining showed that
roxithromycin decreased SM cytotoxicity in both SAE and BTE cells. Also,
roxithromycin inhibited the SM-stimulated overproduction of the proinflammatory
cytokines IL-1 beta, IL-6, IL-8 and TNF at both the protein level and the mRNA
level, as measured by either enzyme-linked immunosorbent assay (ELISA) or real-time
RT-PCR. In addition, roxithromycin inhibited the SM-induced overexpression of iNOS,
as revealed by immunocytochemical analysis using quantum dots as the fluorophore.
Conclusion: The present study demonstrates that roxithromycin has inhibitory
effects on the cytotoxicity and inflammation provoked by SM in human respiratory
epithelial cells. The decreased cytotoxicity in roxithromycin-treated cells likely
depends on the ability of the macrolide to down-regulate the production of
proinflammatory cytokines and/or mediators. The results obtained in this study
suggest that macrolide antibiotics may serve as potential vesicant respiratory
therapeutics through mechanisms independent of their antibacterial activity.
AN - WOS:000247145400001
AU - Gao, X.
AU - Ray, R.
AU - Xiao, Y.
AU - Barker, P. E.
AU - Ray, P.
C7 - 17
DA - MAY 24
DO - 10.1186/1471-2121-8-17
PY - 2007
SN - 1471-2121
ST - Inhibition of sulfur mustard-induced cytotoxicity and inflammation by the
macrolide antibiotic roxithromycin in human respiratory epithelial cells
T2 - BMC CELL BIOLOGY
TI - Inhibition of sulfur mustard-induced cytotoxicity and inflammation by the
macrolide antibiotic roxithromycin in human respiratory epithelial cells
VL - 8
ID - 6303
ER -
TY - JOUR
AB - Objectives: Strong evidence has proven that cerebral hypoperfusion is closely
related to Parkinson's disease (PD)with cognitive impairment. The aim of this study
was to investigate the effect of chronic cerebral hypoperfusion (CCH)on cognitive
dysfunction, structural abnormalities of the hippocampus and white matter (WM), and
levels of inflammatory cytokines in control and 1-methyl-4-phenyl-1,2,3,6-
tetrahydropyridine (MPTP)-lesioned mouse models. Methods: In the present study,
bilateral common carotid artery stenosis (BCCAS)was performed using microcoils, and
the cognitive function and WM lesions (WMLs)after BCCAS were compared between
microcoil with 0.18 mm and 0.20 mm diameters. CCH and MPTP-lesioned mice were
induced by intraperitoneal injection of MPTP and BCCAS. These mice were further
divided into seven groups: a control group, sham-operated group, BCCAS group, PD
with normal cognition (PDCN)group, PD with mild cognitive impairment (PDMCI)group,
PDCN + BCCAS group, and PDMCI + BCCAS group. After 28 days of BCCAS, the mice were
tested through pole-climbing experiments and by TUNEL, Nissl, and Bielschowsky
silver staining. Immunohistochemistry was used to evaluate lesions in the
dopaminergic (DAergic)nigrostriatal system and the number of activated microglia.
Chip-based liquid chromatography was employed to measure the levels of inflammatory
cytokines in the plasma. Results: The results indicated that the histological
results of the 0.18 mm microcoil were superior to that of the 0.20 mm microcoil.
Based on these finding, BCCAS impaired the climbing ability of MPTP-lesioned PD
mice. Moreover, immunohistochemistry for tyrosine hydroxylase (TH)revealed a
significant reduction in the number of DAergic neurons in the substantia nigra of
PD mice following BCCAS, particularly in the PDMCI + BCCAS group. In addition,
Nissl, TUNEL and Bielschowsky silver staining confirmed decreased hippocampal
neuron numbers, increased neuronal apoptosis and more significant WM fiber damage
in the corpus callosum of the PDMCI + BCCAS group. Finally,immunohistochemistry for
ionized calcium binding adaptor molecule-1 (Iba-1)and chip-based liquid
chromatography revealed significantly increased microglial activation (P < 0.01)and
significantly increased levels of interleukin-1β (IL-1β)and interferon-γ (IFN-γ)(P
< 0.05)in the PDMCI + BCCAS group compared with the corresponding levels in the
PDCN + BCCAS group. Conclusions: Cerebral hypoperfusion can aggravate the cognitive
impairment in MPTP-lesioned PD mice. This finding may be related to the
hypoperfusion-mediated deterioration of neuroinflammation, aggravation of WM
damage, and induction of hippocampal neuron apoptosis in PD mice. © 2019 Elsevier
B.V.
AU - Gao, Y.
AU - Tang, H.
AU - Nie, K.
AU - Zhu, R.
AU - Gao, L.
AU - Feng, S.
AU - Wang, L.
AU - Zhao, J.
AU - Huang, Z.
AU - Zhang, Y.
C7 - 111885
DB - Scopus
DO - 10.1016/j.bbr.2019.03.054
KW - Cerebral hypoperfusion
Cerebral microvascular injury
Microglial activation
MPTP
Parkinson's disease
White matter lesion
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Animals
Apoptosis
Brain
Brain Ischemia
Carotid Stenosis
Cognitive Dysfunction
Corpus Callosum
Corpus Striatum
Dopamine
Dopaminergic Neurons
Hippocampus
Male
Mice
Mice, Inbred C57BL
MPTP Poisoning
Nervous System Diseases
Parkinson Disease
Substantia Nigra
Tyrosine 3-Monooxygenase
White Matter
1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine
adaptor protein
cytokine
gamma interferon
interleukin 1beta
ionized calcium binding adaptor molecule 1
unclassified drug
dopamine
animal cell
animal experiment
animal model
animal tissue
Article
brain damage
carotid artery obstruction
cell count
cerebrovascular disease
chronic cerebral hypoperfusion
chronic disease
cognitive defect
controlled study
corpus callosum
cytokine blood level
experimental study
functional assessment
hippocampus
histopathology
left common carotid artery
liquid chromatography
microglia
mild cognitive impairment
mouse
nerve cell stimulation
neuroapoptosis
nigroneostriatal system
nonhuman
physical performance
priority journal
protein blood level
right common carotid artery
sham procedure
white matter lesion
animal
apoptosis
brain
brain ischemia
C57BL mouse
corpus striatum
disease model
drug effect
intoxication
male
metabolism
neurologic disease
Parkinson disease
pathology
pathophysiology
substantia nigra
white matter
M3 - Article
PY - 2019
ST - Hippocampal damage and white matter lesions contribute to cognitive
impairment in MPTP-lesioned mice with chronic cerebral hypoperfusion
T2 - Behavioural Brain Research
TI - Hippocampal damage and white matter lesions contribute to cognitive
impairment in MPTP-lesioned mice with chronic cerebral hypoperfusion
85065534255&doi=10.1016%2fj.bbr.2019.03.054&partnerID=40&md5=fd194b93fc3c18e8bbc81c
7880881dee
VL - 368
ID - 5452
ER -
TY - JOUR
AB - Nanotechnology is an emerging science involving the manipulation of matter on
the nanometer scale. Nanoparticles (NPs) are engineered structures with at least
one dimension of 100 nm or less. These materials are progressively being used for
commercial purposes and being incorporated into everyday manufactured articles at
an increasing rate. These products include consumer items such as pharmaceuticals,
cosmetics, food, food packaging, and household products, among others. The same
unique physical and chemical properties that make NPs so attractive may be
associated with their potentially hazardous effects on cells and tissues. Despite
the large benefit ensured from the application of nanotechnology, many issues
related to NP behavior and adverse effects are not fully understood or should be
examined anew. The traditional hypothesis that NPs exhibit different or additional
hazards due to their "nano" size has been challenged in recent years, and NP
categorization according to their properties and toxicity mechanism has been
proposed instead. Possible undesirable results of these capabilities are harmful
interactions with biological systems and the environment, with the potential to
generate toxicity. Both in vivo and in vitro studies have shown that NPs are
closely associated with toxicity by increasing intracellular reactive oxygen
species (ROS) levels, and/or the levels of pro-inflammatory mediators. This review
summarizes available data on NP toxicity in biological systems, with particular
focus on oxidative stress and inflammation as the main mechanisms that lead to
adverse health effects following NP exposure.
AN - WOS:000677725700001
AU - Garces, M.
AU - Caceres, L.
AU - Chiappetta, D.
AU - Magnani, N.
AU - Evelson, P.
C6 - JUL 2021
DA - AUG 28
DO - 10.1039/d1nj01415c
IS - 32
PY - 2021
SN - 1144-0546
1369-9261
SP - 14328-14344
ST - Current understanding of nanoparticle toxicity mechanisms and interactions
with biological systems
TI - Current understanding of nanoparticle toxicity mechanisms and interactions
with biological systems
VL - 45
ID - 6489
ER -
TY - JOUR
AB - The aim of this experiment was to investigate the effect of a glass ionomer
cement with silver particles (Ketac Silver) on pulp tissue. Class V cavities were
prepared in 60 healthy teeth scheduled for extraction for orthodontic reasons. A
base of Dycal was placed in each cavity. Thirty teeth were filled with Ketac Silver
and 30 with ZOE. Ten teeth of each group were extracted 15, 30, and 60 days later.
At 15 days, the pulps in the Ketac group showed vacuolization and disruption of the
odontoblastic layer, edema, vasodilation, chronic inflammatory infiltrate (CII, and
necrosis. At 30 days, a necrotic odontoblastic layer, severe CII, and extensive
areas of necrosis were seen. At 60 days, pulp tissue was almost completely
necrotic. The ZOE control group showed a slight CII at 15 days. Our results suggest
that under these experimental conditions, this cement is highly toxic and induces
irreversible pulpal damage. Copyright © 1997 by The American Association of
Endodontists.
AU - Garcés-Ortíz, M.
DB - Scopus
DO - 10.1016/S0099-2399(97)80185-9
IS - 6
KW - Cermet Cements
Dental Pulp
Dental Pulp Necrosis
Dentin Sensitivity
Humans
Odontoblasts
cement
article
dentin sensitivity
drug effect
human
odontoblast
tooth pulp
tooth pulp disease
M3 - Article
PY - 1997
SP - 371-373
ST - Cytotoxicity of ketac silver cement
TI - Cytotoxicity of ketac silver cement
0031151296&doi=10.1016%2fS0099-2399%2897%2980185-
9&partnerID=40&md5=1755b52239f241fefe6f67af67b855a2
VL - 23
ID - 5827
ER -
TY - JOUR
AB - The aim of this experiment was to investigate the effect of a glass ionomer
cement with silver particles (Ketac Silver) on pulp tissue. Class V cavities were
prepared in 60 healthy teeth scheduled for extraction for orthodontic reasons. A
base of Dycal was placed in each cavity. Thirty teeth were filled with Ketac Silver
and 30 with ZOE. Ten teeth of each group were extracted 15, 30, and 60 days later.
At 15 days, the pulps in the Ketac group showed vacuolization and disruption of the
odontoblastic layer, edema, vasodilation, chronic inflammatory infiltrate (CII),
and necrosis. At 30 days, a necrotic odontoblastic layer, severe CII, and extensive
areas of necrosis were seen. At 60 days, pulp tissue was almost completely
necrotic. The ZOE control group showed a slight CII at 15 days. Our results suggest
that under these experimental conditions, this cement is highly toxic and induces
irreversible pulpal damage.
AN - WOS:A1997XB72600005
AU - GarcesOrtiz, M.
AU - LedesmaMontes, C.
DA - JUN
DO - 10.1016/S0099-2399(97)80185-9
IS - 6
PY - 1997
SN - 0099-2399
SP - 371-373
ST - Cytotoxicity of Ketac Silver cement
T2 - JOURNAL OF ENDODONTICS
TI - Cytotoxicity of Ketac Silver cement
VL - 23
ID - 6219
ER -
TY - JOUR
AB - Biomimetic nanomaterials (BNMs) are functional materials containing nanoscale
components and having structural and technological similarities to natural
(biogenic) prototypes. Despite the fact that biomimetic approaches in materials
technology have been used since the second half of the 20th century, BNMs are still
at the forefront of materials science. This review considered a general
classification of such nanomaterials according to the characteristic features of
natural analogues that are reproduced in the preparation of BNMs, including
biomimetic structure, biomimetic synthesis, and the inclusion of biogenic
components. BNMs containing magnetic, metal, or metal oxide organic and ceramic
structural elements (including their various combinations) were considered
separately. The BNMs under consideration were analyzed according to the declared
areas of application, which included tooth and bone reconstruction, magnetic and
infrared hyperthermia, chemo- and immunotherapy, the development of new drugs for
targeted therapy, antibacterial and anti-inflammatory therapy, and bioimaging. In
conclusion, the authors' point of view is given about the prospects for the
development of this scientific area associated with the use of native, genetically
modified, or completely artificial phospholipid membranes, which allow combining
the physicochemical and biological properties of biogenic prototypes with high
biocompatibility, economic availability, and scalability of fully synthetic
nanomaterials.
AN - WOS:000833707400001
AU - Gareev, K. G.
AU - Grouzdev, D. S.
AU - Koziaeva, V. V.
AU - Sitkov, N. O.
AU - Gao, H. L.
AU - Zimina, T. M.
AU - Shevtsov, M.
C7 - 2485
DA - JUL
DO - 10.3390/nano12142485
IS - 14
PY - 2022
SN - 2079-4991
ST - Biomimetic Nanomaterials: Diversity, Technology, and Biomedical Applications
T2 - NANOMATERIALS
TI - Biomimetic Nanomaterials: Diversity, Technology, and Biomedical Applications
VL - 12
ID - 6644
ER -
TY - JOUR
AB - The aim of this review is to evaluate the possibility of delivering a silver-
acid complex via a Trojan-horse mechanism for managing periodontits. We theroised
that the complex could be an effective treatment option for bacterial inflammatory
processes in the oral cavity. Searches were conducted using MEDLINE, Embase, Web of
Science Core Collection, and Goo-gle Scholar search engines. We also reviewed
several reference lists of the included studies or relevant reviews identified by
the search. By using Medical Subject Headings (MeSH) ter-minology, a comprehensive
search was performed for the following keywords: silver, folic acid, periodontitis,
macrophages, Trojan-horse mechanism, toxicity, and targeting. Using the keywords
mentioned earlier, we selected 110 articles and after appropriate elimination the
review was written based on 37 papers. Accordingly the we noted that silver isons
were an effective approach to kill oral pathogens. Secondly the Trojan-horse
mechanism. could be used by macrophages (as the Trojan horse) to deliver silver
ions in large quantities to the inflammatory focus to kill the periodontopathogens.
The Trojan-horse mechanism has never been described in the field of dentistry
before. The proposed novel approach using the principle of Trojan Horse delivery of
drugs/chemicals could be used to manage oral inflam-matory conditions. This method
can be used to supplement regular treatments. (c) 2022 The Authors. Published by
Elsevier Inc. on behalf of FDI World Dental Federation. This is an open access
article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-
nd/4.0/)
AN - WOS:001008767600001
AU - Geczi, Z.
AU - Roth, I.
AU - Kohidai, Z.
AU - Kohidai, L.
AU - Mukaddam, K.
AU - Hermann, P.
AU - Vegh, D.
AU - Zelles, T.
C6 - MAY 2023
DA - JUN
DO - 10.1016/j.identj.2022.08.003
IS - 3
PY - 2023
SN - 0020-6539
1875-595X
SP - 346-353
ST - The use of Trojan-horse drug delivery system in managing periodontitis
T2 - INTERNATIONAL DENTAL JOURNAL
TI - The use of Trojan-horse drug delivery system in managing periodontitis
VL - 73
ID - 6397
ER -
TY - JOUR
AB - Background: Leishmaniasis is an infectious disease caused by parasites of the
genus Leishmania and presents different clinical manifestations. The adverse
effects, immunosuppression and resistant strains associated with this disease
necessitate the development of new drugs. Nanoparticles have shown potential as
alternative antileishmanial drugs. We showed in a previous study the biosynthesis,
characterization and ideal concentration of a nanocomposite that promoted
leishmanicidal activity. In the present study, we conducted a specific analysis to
show the mechanism of action of AgNP-PVP-MA (silver nanoparticle–
polyvinylpyrrolidone-[meglumine antimoniate (Glucantime®)]) nanocomposite during
Leishmania amazonensis infection in vitro. Results: Through ultrastructural
analysis, we observed significant alterations, such as the presence of small
vesicles in the flagellar pocket and in the extracellular membrane, myelin-like
structure formation in the Golgi complex and mitochondria, flagellum and plasma
membrane rupture, and electrodense material deposition at the edges of the parasite
nucleus in both evolutive forms. Furthermore, the Leishmania parasite infection
index in macrophages decreased significantly after treatment, and nitric oxide and
reactive oxygen species production levels were determined. Additionally,
inflammatory, and pro-inflammatory cytokine and chemokine production levels were
evaluated. The IL-4, TNF-α and MIP-1α levels increased significantly, while the IL-
17 A level decreased significantly after treatment. Conclusions: Thus, we
demonstrate in this study that the AgNP-PVP-MA nanocomposite has leishmanial
potential, and the mechanism of action was demonstrated for the first time, showing
that this bioproduct seems to be a potential alternative treatment for
leishmaniasis. © 2021, The Author(s).
AU - Gélvez, A. P. C.
AU - Diniz Junior, J. A. P.
AU - Brígida, R. T. S. S.
AU - Rodrigues, A. P. D.
C7 - 211
DB - Scopus
DO - 10.1186/s12866-021-02267-2
IS - 1
KW - AgNP-PVP-MA nanocomposite
Antileishmanial
Cytokine production
Leishmaniasis
Ultrastructural alterations
Animals
Antiprotozoal Agents
Cells, Cultured
In Vitro Techniques
Leishmania
Macrophages
Meglumine Antimoniate
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Nanocomposites
Povidone
Silver
amphotericin B
CD14 antigen
gamma interferon
interleukin 17
interleukin 1beta
interleukin 4
interleukin 6
meglumine antimonate
nanocomposite
nanoparticle
nitric oxide
RANTES
silver nanoparticle
antiprotozoal agent
metal nanoparticle
povidone
silver
animal cell
animal experiment
Article
Aspergillus flavus
biological activity
biosynthesis
cell activation
cell membrane
cell structure
cell vacuole
controlled study
cytokine production
cytotoxicity
cytotoxicity assay
electron microscopy
flow cytometry
gene expression
immune response
Leishmania amazonensis
leishmaniasis
leishmanicidal activity
limit of detection
macrophage
membrane rupture
mitochondrion
morphological adaptation
mouse
multivesicular body
nonhuman
oxidative stress
oxygen consumption
phagocytosis
promastigote
protein expression
Trypanosoma cruzi
ultrastructural
animal
Bagg albino mouse
cell culture
chemistry
drug effect
drug therapy
in vitro study
parasitology
pharmacology
physiology
ultrastructure
M3 - Article
PY - 2021
ST - AgNP-PVP-meglumine antimoniate nanocomposite reduces Leishmania amazonensis
infection in macrophages
T2 - BMC Microbiology
TI - AgNP-PVP-meglumine antimoniate nanocomposite reduces Leishmania amazonensis
infection in macrophages
85110045001&doi=10.1186%2fs12866-021-02267-
2&partnerID=40&md5=6c820eb6a685091175e8268bd81acee4
VL - 21
ID - 5256
ER -
TY - JOUR
AB - Activated macrophages can promote regeneration of CNS axons. However,
macrophages also release factors that kill neurons. These opposing functions are
likely induced simultaneously but are rarely considered together in the same
experimental preparation. A goal of this study was to unequivocally document the
concurrent neurotoxic and neuroregenerative potential of activated macrophages. To
do so, we quantified the length and magnitude of axon growth from enhanced green
fluorescent protein-expressing dorsal root ganglion (DRG) neurons transplanted into
the spinal cord in relationship to discrete foci of activated macrophages.
Macrophages were activated via intraspinal injections of zymosan, a potent
inflammatory stimulus known to increase axon growth and cause neurotoxicity. Using
this approach, a significant increase in axon growth up to macrophage foci was
evident. Within and adjacent to macrophages, DRG and spinal cord axons were
destroyed. Macrophage toxicity became more evident when zymosan was injected closer
to DRG soma. Under these conditions, DRG neurons were killed or their ability to
extend axons was dramatically impaired. The concurrent induction of pro-
regenerative and neurotoxic functions in zymosan-activated macrophages (ZAMs) was
confirmed in vitro using DRG and cortical neurons. Importantly, the ability of ZAMs
to stimulate axon growth was transient; prolonged exposure to factors produced by
ZAMs enhanced cell death and impaired axon growth in surviving neurons.
Lipopolysaccharide, another potent macrophage activator, elicited a florid
macrophage response, but without enhancing axon growth or notable toxicity.
Together, these data show that a single mode of activation endows macrophages with
the ability to simultaneously promote axon regeneration and cell killing.
AN - WOS:000264544900030
AU - Gensel, J. C.
AU - Nakamura, S.
AU - Guan, Z.
AU - van Rooijen, N.
AU - Ankeny, D. P.
AU - Popovich, P. G.
DA - MAR 25
DO - 10.1523/JNEUROSCI.3992-08.2009
IS - 12
PY - 2009
SN - 0270-6474
SP - 3956-3968
ST - Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity
T2 - JOURNAL OF NEUROSCIENCE
TI - Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity
VL - 29
ID - 6800
ER -
TY - JOUR
AB - Previous work indicates that silver nanoparticles (AgNPs) given IP to mice
alter the regulation of inflammation- and oxidative stress-related genes in brain.
Here we assessed the distribution and toxic potential of AgNP following intranasal
(IN) exposure. Adult male C57BL/6J mice received 25-nm AgNP (100 or 500 mg/kg) once
IN. After 1 or 7 days, histopathology of selected organs was performed, and tissue
reduced glutathione (GSH) levels were measured as an indicator of oxidative stress.
Aggregated AgNP were found in spleen, lung, kidney, and nasal airway by routine
light microscopy. Splenic AgNP accumulation was greatest in red pulp and occurred
with modestly reduced cellularity and elevated hemosiderin deposition. Aggregated
AgNP were not associated with microscopic changes in other tissues except for nasal
mucosal erosions. Autometallography revealed AgNP in olfactory bulb and the lateral
brain ventricles. Neither inflammatory cell infiltrates nor activated microglia
were detected in brains of AgNP-treated mice. Elevated tissue GSH levels was
observed in nasal epithelia (both doses at 1 day, 500 mg/kg at 7 days) and blood
(500 mg/kg at 7 days). Therefore, IN administration of AgNP permits systemic
distribution, produces reversible oxidative stress in the nose and in blood, and
mildly enhances macrophage-mediated erythrocyte destruction in the spleen. © 2012
by The Author(s).
AU - Genter, M. B.
AU - Newman, N. C.
AU - Shertzer, H. G.
AU - Ali, S. F.
AU - Bolon, B.
DB - Scopus
DO - 10.1177/0192623312444470
IS - 7
KW - autometallography
glutathione
intranasal instillation
nanoparticles
silver
spleen
Administration, Intranasal
Air Pollutants, Occupational
Animals
Brain
Erythrocytes
Glutathione
Macrophages
Male
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Nasal Mucosa
Oxidative Stress
Silver Compounds
Tissue Distribution
Toxicity Tests
Mus
silver nanoparticle
animal experiment
animal model
animal tissue
article
brain ventricle
cell destruction
cell infiltration
controlled study
drug accumulation
drug distribution
drug effect
erythrocyte
histopathology
inflammatory cell
kidney
lung
macrophage
male
microglia
microscopy
mouse
nonhuman
nose mucosa
olfactory bulb
oxidative stress
particle size
priority journal
protein blood level
single drug dose
tissue level
upper respiratory tract
M3 - Article
PY - 2012
SP - 1004-1013
ST - Distribution and systemic effects of intranasally administered 25 nm silver
nanoparticles in adult mice
T2 - Toxicologic Pathology
TI - Distribution and systemic effects of intranasally administered 25 nm silver
nanoparticles in adult mice
84865578615&doi=10.1177%2f0192623312444470&partnerID=40&md5=7a065d52e737b7ed4fc645c
85f95a006
VL - 40
ID - 5661
ER -
TY - JOUR
AB - Background: The increased incorporation of silver nanoparticles (Ag NPs) into
consumer products makes the characterization of potential risk for humans and other
organisms essential. The oral route is an important uptake route for NPs, therefore
the study of the gastrointestinal tract in respect to NP uptake and toxicity is
very timely. The aim of the present study was to evaluate the effects of Ag NPs and
ions on a Caco-2/TC7:HT29-MTX intestinal co-culture model with mucus secretion,
which constitutes an important protective barrier to exogenous agents in vivo and
may strongly influence particle uptake. Methods: The presence of the mucus layer
was confirmed with staining techniques (alcian blue and toluidine blue). Mono and
co-cultures of Caco-2/TC7 and HT29-MTX cells were exposed to Ag NPs (Ag 20 and 200
nm) and AgNO3 and viability (alamar blue), ROS induction (DCFH-DA assay) and IL-8
release (ELISA) were measured. The particle agglomeration in the media was
evaluated with DLS and the ion release with ultrafiltration and ICP-MS. The effects
of the Ag NPs and AgNO3 on cells in co-culture were studied at a proteome level
with two-dimensional difference in gel electrophoresis (2D-DIGE) followed by Matrix
Assisted Laser Desorption Ionization - Time Of Flight/Time Of Flight
(MALDI-TOF/TOF) mass spectrometry (MS). Intracellular localization was assessed
with NanoSIMS and TEM. Results: The presence of mucus layer led to protection
against ROS and decrease in IL-8 release. Both Ag 20 and 200 nm NPs were taken up
by the cells and Ag NPs 20 nm were mainly localized in organelles with high sulfur
content. A dose-and size-dependent increase in IL-8 release was observed with a
lack of cytotoxicity and oxidative stress. Sixty one differentially abundant
proteins were identified involved in cytoskeleton arrangement and cell cycle,
oxidative stress, apoptosis, metabolism/detoxification and stress. Conclusions: The
presence of mucus layer had an impact on modulating the induced toxicity of NPs.
NP-specific effects were observed for uptake, pro-inflammatory response and changes
at the proteome level. The low level of overlap between differentially abundant
proteins observed in both Ag NPs and AgNO3 treated co-culture suggests size-
dependent responses that cannot only be attributed to soluble Ag.
AN - WOS:000370227900001
AU - Georgantzopoulou, A.
AU - Serchi, T.
AU - Cambier, S.
AU - Leclercq, C. C.
AU - Renaut, J.
AU - Shao, J.
AU - Kruszewski, M.
AU - Lentzen, E.
AU - Grysan, P.
AU - Eswara, S.
AU - Audinot, J. N.
AU - Contal, S.
AU - Ziebel, J.
AU - Guignard, C.
AU - Hoffmann, L.
AU - Murk, A. J.
AU - Gutleb, A. C.
C7 - 9
DA - FEB 17
DO - 10.1186/s12989-016-0117-9
PY - 2016
SN - 1743-8977
ST - Effects of silver nanoparticles and ions on a co-culture model for the
TI - Effects of silver nanoparticles and ions on a co-culture model for the
VL - 13
ID - 5973
ER -
TY - JOUR
AB - Background: The increased incorporation of silver nanoparticles (Ag NPs) into
consumer products makes the characterization of potential risk for humans and other
organisms essential. The oral route is an important uptake route for NPs, therefore
the study of the gastrointestinal tract in respect to NP uptake and toxicity is
very timely. The aim of the present study was to evaluate the effects of Ag NPs and
ions on a Caco-2/TC7:HT29-MTX intestinal co-culture model with mucus secretion,
which constitutes an important protective barrier to exogenous agents in vivo and
may strongly influence particle uptake. Methods: The presence of the mucus layer
was confirmed with staining techniques (alcian blue and toluidine blue). Mono and
co-cultures of Caco-2/TC7 and HT29-MTX cells were exposed to Ag NPs (Ag 20 and 200
nm) and AgNO3 and viability (alamar blue), ROS induction (DCFH-DA assay) and IL-8
release (ELISA) were measured. The particle agglomeration in the media was
evaluated with DLS and the ion release with ultrafiltration and ICP-MS. The effects
of the Ag NPs and AgNO3 on cells in co-culture were studied at a proteome level
with two-dimensional difference in gel electrophoresis (2D-DIGE) followed by Matrix
Assisted Laser Desorption Ionization - Time Of Flight/ Time Of Flight
(MALDI-TOF/TOF) mass spectrometry (MS). Intracellular localization was assessed
with NanoSIMS and TEM. Results: The presence of mucus layer led to protection
against ROS and decrease in IL-8 release. Both Ag 20 and 200 nm NPs were taken up
by the cells and Ag NPs 20 nm were mainly localized in organelles with high sulfur
content. A dose- and size-dependent increase in IL-8 release was observed with a
lack of cytotoxicity and oxidative stress. Sixty one differentially abundant
proteins were identified involved in cytoskeleton arrangement and cell cycle,
oxidative stress, apoptosis, metabolism/detoxification and stress. Conclusions: The
presence of mucus layer had an impact on modulating the induced toxicity of NPs.
NP-specific effects were observed for uptake, pro-inflammatory response and changes
at the proteome level. The low level of overlap between differentially abundant
proteins observed in both Ag NPs and AgNO3 treated co-culture suggests size-
dependent responses that cannot only be attributed to soluble Ag. © 2016
Georgantzopoulou et al.
AU - Georgantzopoulou, A.
AU - Serchi, T.
AU - Cambier, S.
AU - Leclercq, C. C.
AU - Renaut, J.
AU - Shao, J.
AU - Kruszewski, M.
AU - Lentzen, E.
AU - Grysan, P.
AU - Eswara, S.
AU - Audinot, J. N.
AU - Contal, S.
AU - Ziebel, J.
AU - Guignard, C.
AU - Hoffmann, L.
AU - Murk, A. T. J.
AU - Gutleb, A. C.
C7 - 9
DB - Scopus
DO - 10.1186/s12989-016-0117-9
IS - 1
KW - Intestinal co-culture
Mucus layer
Proteomics
Toxicology
Caco-2 Cells
Cell Survival
Epithelial Cells
HT29 Cells
Humans
Interleukin-8
Intestinal Mucosa
Metal Nanoparticles
Mucus
Oxidative Stress
Risk Assessment
Silver
Silver Nitrate
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
autacoid
IL8 protein, human
interleukin 8
metal nanoparticle
silver
silver nitrate
Caco-2 cell line
cell survival
coculture
comparative study
dose response
drug effects
epithelium cell
HT-29 cell line
human
intestine mucosa
matrix-assisted laser desorption-ionization mass spectrometry
metabolism
mucus
oxidative stress
pathology
procedures
proteomics
risk assessment
secretion (process)
M3 - Article
PY - 2016
ST - Effects of silver nanoparticles and ions on a co-culture model for the
TI - Effects of silver nanoparticles and ions on a co-culture model for the
84957959737&doi=10.1186%2fs12989-016-0117-
9&partnerID=40&md5=6ca826a0a5c98b9bf31d1198242720f2
VL - 13
ID - 5493
ER -
TY - JOUR
AB - Cell cultures of human gingival fibroblasts obtained from healthy patients
were used to evaluate the toxicity of six different endodontic cements: AH-26, Pulp
Canal Sealer, Rocanal-R2, Rocanal-R3, Bioseal, and Endomethasone. The toxicity was
determined by measuring spectrophotometrically at 405 nm the colorimetric reaction
of N-acetyl-β-hexosaminidase, an endogenous enzyme, with the chromogenic substrate
[p-nitrophenol-N-acetyl-β-D-glucosamide (NAG)]. Severe cytotoxicity was observed in
the 1- and 2-wk test solutions of AH-26. Pulp Canal Sealer and Endomethasone showed
low cytotoxicity in the 1- and 2-wk test solutions at 24, 48, and 72 h. Moderate
cytotoxicity was observed in the 1- and 2-wk test solutions of Bioseal, except at
48 and 72 h of 1-wk test solutions. Rocanal-R2 showed severe cytotoxicity in the 1-
wk test solutions at 48 and 72 h, and in the 2-wk test solutions at 24, 48, and 72
h. Moderate cytotoxicity was seen in the 1- and 2-wk test solutions of Rocanal-R3
only at 24 h. © 1995 The American Association of Endodontists.
AU - Gerosa, R.
AU - Menegazzi, G.
AU - Borin, M.
AU - Cavalleri, G.
DB - Scopus
DO - 10.1016/S0099-2399(06)81525-6
IS - 9
beta-N-Acetylhexosaminidase
Bismuth
Cell Survival
Cells, Cultured
Colorimetry
Dexamethasone
Drug Combinations
Epoxy Resins
Fibroblasts
Formaldehyde
Gingiva
Humans
Hydrocortisone
Methenamine
Phenols
Silver
Thymol
Titanium
Bioseal
bismuth
dexamethasone
drug derivative
epoxy resin
formaldehyde
hydrocortisone
Kerr Pulp Canal Sealer
methenamine
phenol derivative
Rocanal
silver
thymol
titanium
zinc oxide eugenol
article
cell culture
cell survival
colorimetry
comparative study
cytology
drug combination
drug effect
enzymology
fibroblast
gingiva
human
metabolism
M3 - Article
PY - 1995
SP - 446-448
ST - Cytotoxicity evaluation of six root canal sealers
TI - Cytotoxicity evaluation of six root canal sealers
0029365027&doi=10.1016%2fS0099-2399%2806%2981525-
6&partnerID=40&md5=c787031e4764dc315e840baaa68a2db4
VL - 21
ID - 5781
ER -
TY - JOUR
AB - Achillea millefolium L. is a medicinal herb with more than 100 antifungal and
antimicrobial active biological compounds. In the present study, A. millefolium
plants at the flowering stage were treated with different concentrations of methyl
jasmonate and silver nanoparticles. Treatments increased lipid peroxidation,
flavonoid content, and yield of essential oils (ca. 230 %), but reduced radical
scavenging capacity and anthocyanin contents of the plants. Cytotoxicity of the
extracts of treated plants against cancer HeLa cells was improved as well.
Production of certain antimicrobial isoprenoids, i.e., camphor was significantly
increased. Certain precious compounds such as allo-ocimene, germacrene, trans-
caryophyllene, and farnesol with antibacterial, antifungal, anti-inflammatory, and
anticancer effects were also induced just after elicitation of the plants with
silver nanoparticles and methyl jasmonate. The results suggested silver
nanoparticles as a novel elicitor in plant biotechnology as effective as methyl
jasmonate in order for production of desired secondary metabolites in A.
millefolium.
AU - Ghanati, F.
AU - Bakhtiarian, S.
AU - Parast, B. M.
AU - Behrooz, M. K.
DB - Scopus
DO - 10.13005/bbra/1287
IS - 2
KW - Achillea millefolium L.
Essential oil
Methyl jasmonate (MeJA)
Redox status
Silver nano particles (AgNPS)
Achillea millefolium
Dryobalanops
3 carene
Achillea millefolium extract
allo ocimene
anthocyanin
apigenin
bicyclogermacrene
camphene
camphor
carvyl acetate
caryophyllene
coumarin
drug metabolite
essential oil
farnesol
flavonoid
germacrene D
jasmonic acid methyl ester
junipene
limonene
luteolin
para cymene
para mentha 1,5,8 triene
plant medicinal product
sesquiterpene derivative
silver nanoparticle
terpinene
terpinolene
trans 3 carene 2 ol
unclassified drug
unindexed drug
valencene
Achillea
antifungal activity
Article
controlled study
drug cytotoxicity
drug isolation
female
flowering
HeLa cell line
human
human cell
lipid peroxidation
nanopharmaceutics
plant biotechnology
plant metabolism
M3 - Article
PY - 2014
SP - 391-399
ST - Production of new active phytocompounds by Achillea millefolium L. after
elicitation with silver nanoparticles and methyl jasmonate
T2 - Biosciences Biotechnology Research Asia
TI - Production of new active phytocompounds by Achillea millefolium L. after
elicitation with silver nanoparticles and methyl jasmonate
84913592936&doi=10.13005%2fbbra
%2f1287&partnerID=40&md5=16788ffbf16641337424de4947fae540
VL - 11
ID - 5592
ER -
TY - JOUR
AB - Research to identify and develop compounds that facilitate wound healing is
important, especially for hard-to-heal chronic wounds. PURPOSE: This study was
conducted to investigate the effects of orally administered propolis (a resinous
substance found in beehives), alone and in combination with silver nanoparticles
(SNPs), on the wound healing process in male rats. METHODS: Forty (40) male Wistar
rats were randomly divided into 4 groups of 10 each: 1 control group received no
treatment, and 3 study groups received a daily dose of 1) propolis (100 mg/kg), 2)
propolis + 30 ppm SNPs, or 3) propolis + 60 ppm SNPs. Healing rate was determined
by wound surface area reduction on days 4, 6, 8, and 10 post-surgery. On day 12
after wound creation, histological changes of wound healing, including number of
new vessels, inflammatory cells (neutrophils, eosinophils, and mast cells), and
fibroblasts, were counted based on morphology using a 400x objective lens, and
collagen deposition density was determined using hematoxylin and eosin and
trichrome staining, respectively. The histological scores were based on a 0 to 4
scale from lowest to highest amount of improving tissue status and were analyzed
using one-way analysis of variance, Tukey test, Kruskal-Wallis test, t test, and
Mann-Whitney U test to examine differences among the groups. Significance was set
at P <.05. RESULTS: The rate of wound healing was significantly different between
the control and the treated groups on days 4, 6, 8, and 10 (percent change was not
assessed on day 12) post-surgery, especially in the propolis + 30 ppm SNPs group
compared to the control group. This difference was more significant on days 6
(wound healing percentage [WHP]: 75% and 45%) and 8 (WHP: 88% and 65%) post-surgery
(P <.001). Mean neutrophil count on day 12 was highest in the control (34.8 +/-
2.97) and lowest in the propolis + 30 ppm SNPs group (16.55 +/- 2.12). The number
of eosinophils on day 12 was considerably higher in the control group (1.05 +/- 4)
compared to those in the propolis group (3 +/- 0.70), propolis + 30 ppm SNPs group
(60/0 +/- 1/1), and propolis + 60 ppm SNPs group (0.5 +/- 0.52) (P <.001). Mean
propolis + 30 ppm SNPs scores for epithelialization and granulation tissue
formation were 3 and 4, respectively; in the propolis + 60 ppm SNPs, scores were 2
and 3, respectively; in the propolis alone group scores were 2 and 3, respectively
(statistical significance not computed for semiquantitative values). The highest
fibroblast count was in the propolis + 30 ppm SNPs group (114.44 +/- 3.90) compared
to control group (73.2 +/- 2.8); P <.001). The difference in collagen fiber density
scores was also significant: 1.2 +/- 0.42 in the control and 3.66 +/- 0.50 in the
propolis + 30 ppm SNPs group; (P <.001). The mean of collagen fiber density in the
propolis + 60 ppm SNPs group was 2.63 +/- 0.51. CONCLUSION: Oral propolis alone and
in combination with 30 ppm SNPs appears to provide anti-inflammatory effects and
increase fibroblast proliferation and collagen deposition in experimental wounds,
which may explain the observed differences in healing. Propolis + 60 ppm SNPs
appears to have a cytotoxic effect. Research confirming these results and that
examines toxicity levels in animals and humans is needed.
AN - WOS:000589352700003
AU - Gheib, N.
AU - Farzam, A.
AU - Habibian, Z.
AU - Samiee-Rad, F.
DA - APR
DO - 10.25270/wmp.2020.4.3846
IS - 4
PY - 2020
SN - 2640-5237
2640-5245
SP - 38-46
ST - The Effect of Oral Consumption of Propolis Alone and in Combination With
Silver Nanoparticles on Wound Healing in Male Wistar Rats
T2 - WOUND MANAGEMENT & PREVENTION
TI - The Effect of Oral Consumption of Propolis Alone and in Combination With
Silver Nanoparticles on Wound Healing in Male Wistar Rats
VL - 66
ID - 6185
ER -
TY - JOUR
AB - Research to identify and develop compounds that facilitate wound healing is
important, especially for hard-to-heal chronic wounds. PURPOSE: This study was
conducted to investigate the effects of orally administered propolis (a resinous
substance found in beehives), alone and in combination with silver nanoparticles
(SNPs), on the wound healing process in male rats. METHODS: Forty (40) male Wistar
rats were randomly divided into 4 groups of 10 each: 1 control group received no
treatment, and 3 study groups received a daily dose of 1) propolis (100 mg/kg), 2)
propolis + 30 ppm SNPs, or 3) propolis + 60 ppm SNPs. Healing rate was determined
by wound surface area reduction on days 4, 6, 8, and 10 post-surgery. On day 12
after wound creation, histological changes of wound healing, including number of
new vessels, inflammatory cells (neutrophils, eosinophils, and mast cells), and
fibroblasts, were counted based on morphology using a 400x objective lens, and
collagen deposition density was determined using hematoxylin and eosin and
trichrome staining, respectively. The histological scores were based on a 0 to 4
scale from lowest to highest amount of improving tissue status and were analyzed
using one-way analysis of variance, Tukey test, Kruskal-Wallis test, t test, and
Mann-Whitney U test to examine differences among the groups. Significance was set
at P <.05. RESULTS: The rate of wound healing was significantly different between
the control and the treated groups on days 4, 6, 8, and 10 (percent change was not
assessed on day 12) post-surgery, especially in the propolis + 30 ppm SNPs group
compared to the control group. This difference was more significant on days 6
(wound healing percentage [WHP]: 75% and 45%) and 8 (WHP: 88% and 65% ) post-
surgery (P <.001). Mean neutrophil count on day 12 was highest in the control (34.8
± 2.97) and lowest in the propolis + 30 ppm SNPs group (16.55 ± 2.12). The number
of eosinophils on day 12 was considerably higher in the control group (1.05 ± 4)
compared to those in the propolis group (3 ± 0.70), propolis + 30 ppm SNPs group
(60/0 ± 1/1), and propolis + 60 ppm SNPs group (0.5 ± 0.52) (P <.001). Mean
propolis + 30 ppm SNPs scores for epithelialization and granulation tissue
formation were 3 and 4, respectively; in the propolis + 60 ppm SNPs, scores were 2
and 3, respectively; in the propolis alone group scores were 2 and 3, respectively
(statistical significance not computed for semiquantitative values). The highest
fibroblast count was in the propolis + 30 ppm SNPs group (114.44 ± 3.90) compared
to control group (73.2 ± 2.8); P <.001). The difference in collagen fiber density
scores was also significant: 1.2 ± 0.42 in the control and 3.66 ± 0.50 in the
propolis + 30 ppm SNPs group; (P <.001). The mean of collagen fiber density in the
propolis + 60 ppm SNPs group was 2.63 ± 0.51. CONCLUSION: Oral propolis alone and
in combination with 30 ppm SNPs appears to provide anti-inflammatory effects and
increase fibroblast proliferation and collagen deposition in experimental wounds,
which may explain the observed differences in healing. Propolis + 60 ppm SNPs
appears to have a cytotoxic effect. Research confirming these results and that
examines toxicity levels in animals and humans is needed. © 2020, HMP Global. All
rights reserved.
AU - Gheibi, N.
AU - Farzam, A.
AU - Habibian, Z.
AU - Samiee-Rad, F.
DB - Scopus
DO - 10.25270/wmp.2020.4.3846
IS - 4
KW - Pharmaceutical preparations
Propolis
Research
Silver
Wound healing
Animals
Female
Rats, Wistar
Silver Nitrate
Soft Tissue Injuries
Wound Healing
propolis
silver nitrate
animal
disease model
drug effect
female
pathophysiology
physiology
soft tissue injury
Wistar rat
wound healing
M3 - Article
PY - 2020
SP - 38-46
ST - The effect of oral consumption of propolis alone and in combination with
silver nanoparticles on wound healing in male wistar rats
T2 - Wound Management and Prevention
TI - The effect of oral consumption of propolis alone and in combination with
silver nanoparticles on wound healing in male wistar rats
85083477176&doi=10.25270%2fwmp.2020.4.3846&partnerID=40&md5=20a2d21556def3f498d73e4
09dfb2739
VL - 66
ID - 5277
ER -
TY - JOUR
AB - Improvement in nanotechnology has identified promising silver nanoparticles
(AgNPs) for many biomedicine applications. To assess the toxicity of silver
nanoparticles in vivo, histopatological examinations of experimental mice were
studied. Forty adult male Sprague-Dawley rats were randomly divided into five
groups and orally treated AgNPs in different doses (30,125,300,700 mg/kg) during
the 28-days. After paraffin embedding and hematoxylin and eosin (H&E) and periodic
acid Schiff (PAS) staining, histopathological changes evaluated in kidney using
light micrographs. The obtained results showed a decrease in diameter of convoluted
tubules, glomerulus diameter, Bowman's space thickness and number of mesangeal
cells in 30 and 125 and 300 mg/kg treated groups. These changes are more evident in
125 mg/kg of AgNPs group (P<0.05). The other histological changes in the tubules of
rats affected by AgNPs included loose of brush border, basement membrane
irregularity, necrosis, vacuolar degeneration and Congestion. The other glomerular
and interstital alterations of rats affected by AgNPs included: basement membrane
thickness, accumulation of mesangial matrix, necrosis and infiltration of
inflammatory cells. These histological alterations were also more evident in rats
exposed to 125 mg/kg of AgNPs. In 700 mg/kg group, no major changes in the
structural component of the kidney were observed while occasional foci of
inflammatory cell infiltrates were present.
AN - WOS:000428114000009
AU - Gherkhbolagh, M. H.
AU - Alizadeh, Z.
AU - Asari, M. J.
AU - Sohrabi, M.
DA - FEB
DO - 10.24896/jrmds.2018619
IS - 1
PY - 2018
SN - 2347-2545
2347-2367
SP - 43-51
ST - In Vivo Induced Nephrotoxicity of Silver Nanoparticles in Rat after Oral
Administration
T2 - JOURNAL OF RESEARCH IN MEDICAL AND DENTAL SCIENCE
TI - In Vivo Induced Nephrotoxicity of Silver Nanoparticles in Rat after Oral
Administration
VL - 6
ID - 5919
ER -
TY - JOUR
AB - Copper nanoparticles are widely utilized in a variety of applications,
including metal catalysts, semiconductors, heat transfer fluids in machine tools,
and even in antibacterial medications. Forty mature healthy Westar rats were
utilized in the current investigation and grouped randomly into four groups (n = 10
rats/group). Group I (G1) was kept as a control group, but G2, G3, and G4 were
intraperitoneally injected with CuO NPs with a dose (5 mg, 10 mg, 25 mg/kg body
weight/day) respectively for 9 days. Rats were sacrificed; then, the livers and
kidneys were dissected and subjected to histopathological and immunohistochemical
examination. Our findings of G2 and G3 revealed mild to moderate degenerative
changes within the hepatic parenchyma, moderate blood vessel congestions, glycogen
depletion, hemosiderosis, and microvesicular steatosis (fatty changes within the
hepatocytes). In addition, at the level of kidney, our examination clarified
moderate degenerations of the renal corpuscles and renal tubules with moderate
swelling and congestions of the glomerulus with moderate vacuolations in the renal
tubules lining epithelium. On the other hand, increasing the dose of CuO NPs, the
toxicity became more obvious, where the liver of G4 revealed severe necrosis of
hepatocytes with completely disorganizations of the hepatic rays, loss of the
hepatic architectures, severe steatosis, severe hemosiderosis, sinusoidal
dilatations with congestions, as well as severe fibrous tissue proliferation with
anti-inflammatory cell infiltrations specially around portal triad with hyperplasia
of bile duct. Meanwhile in kidney, G4 clarified severe necrosis and atrophy of the
renal corpuscles with severe damage of Bowman's capsule leading to completely
disorganization and loss of normal renal cortex architectures, severe congestion of
the glomerulus, severe necrosis of the renal tubules with damage and sloughing for
its lining epithelium, and severe hemorrhage between renal tubules. In addition,
severe and diffuse caspase 3 immunoreactivity were observed within the hepatic and
renal tissues of G4. The present investigation was concluded that the CuO NPs have
a potential toxicological effect on the hepatic and renal tissues that may affect
their functions.-->
AN - WOS:000814967400011
AU - Ghonimi, W. A. M.
AU - Alferah, M. A. Z.
AU - Dahran, N.
AU - El-Shetry, E. S.
C6 - JUN 2022
DA - NOV
DO - 10.1007/s11356-022-21521-2
IS - 54
PY - 2022
SN - 0944-1344
1614-7499
SP - 81923-81937
ST - Hepatic and renal toxicity following the injection of copper oxide
nanoparticles (CuO NPs) in mature male Westar rats: histochemical and caspase 3
immunohistochemical reactivities
TI - Hepatic and renal toxicity following the injection of copper oxide
nanoparticles (CuO NPs) in mature male Westar rats: histochemical and caspase 3
immunohistochemical reactivities
VL - 29
ID - 6412
ER -
TY - JOUR
AB - Nanoparticles (NPs) are being used in several industries worldwide and can
introduce into the human body through different exposure routes, including
inhalation, oral administration, intravenous injection, and intramuscular or
transdermal delivery. The present in vivo study aimed to evaluate the acute oral
toxicological effects of silica (SiO2) and magnesium oxide (MgO) NPs in rats by
using histological, biochemical, and biodistribution parameters. The results
revealed that acute exposure to higher doses of these NPs produced a significant
decrease (p < 0.05) in alanine aminotransferase, alkaline phosphatase serum levels
lactate dehydrogenase, and aspartate aminotransferase. Mild congestion, non-zonal
hepatocellular swelling and degeneration, and apoptotic cells with significant
pyknotic or shrunken nuclei were found in the liver of the treated rats at 2000
mg/kg of the MgO NPs. Moreover, under the microscopic examination, focal
hepatocellular degeneration and necrosis, accumulation of mononuclear inflammatory
cells within the necrotic area and in the portal tract, and severe central vein and
sinusoidal congestion and focal edematous fluids in the hepatic parenchyma were
observed in the livers of the treated rats with 2000 mg/kg of SiO2 NPs. Moreover,
MgO NPs exhibited higher liver and kidney accumulation than SiO2 NPs. In
conclusion, these NPs at a high concentration could have toxicological effects on
rats' liver and kidney tissues. However, further studies require examining the
safety and the other possible toxic effects of these NPs before entering the
consumer market.
AN - WOS:000967260900001
AU - Ghorbani, S.
AU - Moshtaghi, H.
AU - Shekarforoush, S. S.
AU - Gheisari, H. R.
AU - Sedaghati, F.
AU - Nazifi, S.
AU - Ahmadi, N.
C6 - APR 2023
DA - JUN
DO - 10.1007/s40995-023-01451-5
IS - 3
PY - 2023
SN - 2731-8095
2731-8109
SP - 695-705
ST - Histopathologic, Biochemical, and Biodistribution Studies of Orally
Administrated Silica and Magnesium Oxide Nanoparticles in Rats
T2 - IRANIAN JOURNAL OF SCIENCE
TI - Histopathologic, Biochemical, and Biodistribution Studies of Orally
Administrated Silica and Magnesium Oxide Nanoparticles in Rats
VL - 47
ID - 6766
ER -
TY - JOUR
AB - Background: Aegialitis rotundifolia Roxb., is a small mangrove tree or shrub
used traditionally as a potent cure for pain arising from various injuries.
Presently, there is a single scientific report on the wound healing property of
this mangrove species which has been performed in-vitro but there is no in vivo
scientific evidence of the wound healing properties. Further, few reports have
claimed that reduction of pain could accelerate wound healing process and thus, the
present work deals with the development of a topical ointment formulation
incorporated with Aegialitis rotundifolia Roxb., ethanolic leaves extract (ARELE)
which could potentially accelerate healing of excision, incision and burn wound
models in Wistar rats. Methods: Topical ointment containing ARELE was first tested
for their stability (90 days) and possible dermal toxicity using standard
procedures. In the excision wound model, parameters such as in-vivo antioxidant,
acute inflammatory marker (myeloperoxidase), wound microbial load, connective
tissue parameters, and histopathology of healed skin were performed. Incision and
burn (thermal and chemical) wounds were conducted following the standard methods.
Results: The ointment formulations were found to be stable and dermatologically
non-toxic. In the excision wound model, a significant increase in percent wound
contraction was observed for ARELE ointment treated groups which are substantiated
by strong in-vivo antioxidant activity, increased collagen formation, almost normal
skin histology, and reduced myeloperoxidase and microbial colonies. Strong wound
breaking strength was observed in incision wound repair and a significant increase
in percent wound closure in both thermal and chemical burn wound model was recorded
for ARELE ointment treated groups. Conclusion: Therefore, the topical application
of ARELE ointment formulations showed remarkable excision, incision and burn wound
healing in Wistar rats thus showing its potential as a promising wound healing
agent. © 2019 Elsevier GmbH
AU - Ghosh, D.
AU - Mondal, S.
AU - Ramakrishna, K.
C7 - 100168
DB - Scopus
DO - 10.1016/j.wndm.2019.100168
IS - 1
KW - Aegialitis rotundifolia
Burn wound
In-vivo antioxidant activity
Mangrove
Ointment formulation
Wound healing
Aegialitis rotundifolia extract
alcohol
alkaloid
carbohydrate
flavonoid
myeloperoxidase
phytosterol
plant extract
povidone iodine
saponin
sulfadiazine silver
tannin derivative
triterpenoid
unclassified drug
adult
animal experiment
animal model
animal tissue
Article
breaking strength
burn
chemical burn
collagen synthesis
connective tissue
controlled study
drug formulation
drug screening
drug stability
epithelization
histopathology
in vivo study
male
mangrove
nonhuman
ointment
pathogen load
plant leaf
priority journal
rat
skin irritation
skin toxicity
surgical wound
wound closure
wound contraction
wound healing
M3 - Article
PY - 2019
ST - A topical ointment formulation containing leaves extract of Aegialitis
rotundifolia Roxb., accelerates excision, incision and burn wound healing in rats
T2 - Wound Medicine
TI - A topical ointment formulation containing leaves extract of Aegialitis
rotundifolia Roxb., accelerates excision, incision and burn wound healing in rats
85070057071&doi=10.1016%2fj.wndm.2019.100168&partnerID=40&md5=91c57444bc61a660ac399
2b83125b213
VL - 26
ID - 5420
ER -
TY - JOUR
AB - Along the line of recent vaccine advancements, new antiviral therapeutics are
compelling to combat viral infection-related public health crises. Several
properties of silver nanoparticles (AgNPs) such as low level of cytotoxicity, ease
of tunability of the AgNPs in the ultra-small nanoscale size and shape through
different convenient bottom-up chemistry approaches, high penetration of the
composite with drug formulations into host cells has made AgNPs, a promising
candidate for developing antivirals. In this review, we have highlighted the recent
advancements in the AgNPs based nano-formulations to target cellular mechanisms of
viral propagation, immune modulation of the host, and the ability to
synergistically enhance the activity of existing antiviral drugs. On the other
hand, we have discussed the recent advancements on AgNPs based detection of viral
pathogens from clinical samples using inherent physicochemical properties. This
article will provide an overview of our current knowledge on AgNPs based
formulations that has promising potential for developing a counteractive strategy
against emerging and existing viruses.
AN - WOS:000746211100001
AU - Ghosh, U.
AU - Ahammed, K. S.
AU - Mishra, S.
AU - Bhaumik, A.
C6 - JAN 2022
C7 - e202101149
DA - MAR 1
DO - 10.1002/asia.202101149
IS - 5
PY - 2022
SN - 1861-4728
1861-471X
ST - The Emerging Roles of Silver Nanoparticles to Target Viral Life Cycle and
Detect Viral Pathogens
T2 - CHEMISTRY-AN ASIAN JOURNAL
TI - The Emerging Roles of Silver Nanoparticles to Target Viral Life Cycle and
Detect Viral Pathogens
VL - 17
ID - 6162
ER -
TY - JOUR
AB - Hazard studies for nanomaterials (NMs) commonly assess whether they activate
an inflammatory response. Such assessments often rely on rodents, but alternative
models are needed to support the implementation of the 3Rs principles. Zebrafish
(Danio rerio) offer a viable alternative for screening NM toxicity by investigating
inflammatory responses. Here, we used non-protected life stages of transgenic
zebrafish (Tg(mpx:GFP)i114) with fluorescently-labeled neutrophils to assess
inflammatory responses to silver (Ag) and zinc oxide (ZnO) NMs using two
approaches. Zebrafish were exposed to NMs via water following a tail fin injury, or
NMs were microinjected into the otic vesicle. Zebrafish were exposed to NMs at 3
days post-fertilization (dpf) and neutrophil accumulation at the injury or
injection site was quantified at 0, 4, 6, 8, 24, and 48 h post-exposure. Zebrafish
larvae were also exposed to fMLF, LTB4, CXCL-8, C5a, and LPS to identify a suitable
positive control for inflammation induction. Aqueous exposure to Ag and ZnO NMs
stimulated an enhanced and sustained neutrophilic inflammatory response in injured
zebrafish larvae, with a greater response observed for Ag NMs. Following
microinjection, Ag NMs stimulated a time-dependent neutrophil accumulation in the
otic vesicle which peaked at 48 h. LTB4 was identified as a positive control for
studies investigating inflammatory responses in injured zebrafish following aqueous
exposure, and CXCL-8 for microinjection studies that assess responses in the otic
vesicle. Our findings support the use of transgenic zebrafish to rapidly screen the
pro-inflammatory effects of NMs, with potential for wider application in assessing
chemical safety (e.g. pharmaceuticals). © 2022 The Author(s). Published by Informa
UK Limited, trading as Taylor & Francis Group.
AU - Gillies, S.
AU - Verdon, R.
AU - Stone, V.
AU - Brown, D. M.
AU - Henry, T.
AU - Tran, L.
AU - Tucker, C.
AU - Rossi, A. G.
AU - Tyler, C. R.
DB - Scopus
DO - 10.1080/17435390.2022.2088312
IS - 3
KW - 3Rs
inflammation
Nanomaterial
neutrophils
zebrafish larvae
Animals
Animals, Genetically Modified
Larva
Nanostructures
Neutrophils
Zebrafish
Zinc Oxide
complement component C5a
interleukin 8
nanomaterial
silver
water
zinc oxide
animal cell
animal experiment
animal model
Article
caudal fin
chemical safety
fertilization
larva
microinjection
neutrophil
nonhuman
transgenic zebrafish
animal
transgenic animal
zebra fish
M3 - Article
PY - 2022
SP - 333-354
ST - Transgenic zebrafish larvae as a non-rodent alternative model to assess pro-
inflammatory (neutrophil) responses to nanomaterials
T2 - Nanotoxicology
TI - Transgenic zebrafish larvae as a non-rodent alternative model to assess pro-
inflammatory (neutrophil) responses to nanomaterials
85133635141&doi=10.1080%2f17435390.2022.2088312&partnerID=40&md5=3c778daeb5944cf78c
bc30fe4aec0fae
VL - 16
ID - 5103
ER -
TY - JOUR
AB - Wound dressing developed using bioactive materials has been a current area of
research for treating chronic non healing wounds owing to its high demand. Here, we
report the fabrication and evaluation of nanofibrous matrix based wound dressings
using biopolymer poly(vinyl alcohol) (PVA) incorporated with silk sericin (SS). SS
extracted from the cocoons of mulberry variety Botnbyx mori and non-mulberry
variety Antheraea assama has been used to develop two types of blended mats.
Herein, SS based nanofibrous dressings fabricated using electro-spinning technique
were thoroughly characterized and evaluated for wound healing applications. The
developed SS based nanofibrous mats ranged from 130 to 160 nm in diameter with
micro to nanoporous structure. The dressings were endowed with free radical
scavenging capacity, antibacterial activity, swelling capacity, and
biocompatibility due to incorporation of SS. Furthermore, murine fibroblasts (L929)
and human keratinocytes (HaCaT) cultured on the PVA-SS blended mats showed higher
proliferation as compared to pristine PVA mats as observed over a period of 14 days
(p <= 0.01). The blended mats also showed spread out morphology of cells in
comparison to spherical clumps formed on PVA mats. In addition, SS from both silk
types exhibited excellent antioxidant potential without hampering the cell
viability even under H2O2 driven oxidative stress. Moreover, SS (both types)
released from the nanofibrous mats also healed the wounds at thrice the rate of
control under in vitro conditions. Furthermore, subcutaneous implantation of
nanofibrous mats in mice showed in vivo tolerance of the blended nanofibrous mats
observed over four weeks without eliciting any inflammatory reactions to the host
tissue. Taken together, the developed silk sericin-based dressings signify an
attractive substrate for treatment of chronic wounds like diabetic foot ulcers.
AN - WOS:000435619600044
AU - Gilotra, S.
AU - Chouhan, D.
AU - Bhardwaj, N.
AU - Nandi, S. K.
AU - Mandal, B. B.
DA - SEP 1
DO - 10.1016/j.msec.2018.04.077
PY - 2018
SN - 0928-4931
1873-0191
SP - 420-432
ST - Potential of silk sericin based nanofibrous mats for wound dressing
applications
TI - Potential of silk sericin based nanofibrous mats for wound dressing
applications
VL - 90
ID - 6749
ER -
TY - JOUR
AB - Although gold nanoparticles (AuNPs) are currently used in several industrial
products and biomedical applications, information about their biological effects is
very limited. Thus, it is becoming crucial to assess their safety and adequately
investigate the complexity of cell-nanoparticles interactions. In this work, the
Balb/3T3 mouse fibroblast cell line was selected as an in vitro model to study the
effects of AuNPs. Alteration of cellular processes and biochemical pathways caused
by AuNPs exposure was investigated by analysing the differentially expressed
proteome. Of interest was the difference observed in the protein pattern expression
of cells exposed to AuNPs. It was found that 88 and 83 proteins were de-regulated
after exposure to 5 and 15 nm AuNPs, respectively. Analysis of the proteome
revealed that AuNPs triggers several pathways related to cellular growth and
proliferation, cell morphology, cell cycle regulation, cellular function and
maintenance, oxidative stress, and inflammatory response. Moreover, SPR analysis
showed an increase of ECM proteins biosynthesis in cells exposed to AuNPs. We
observed by TEM analysis that NPs are internalized and confined mainly in
autophagosomes. Endoplasmic reticulum stressed and modification at mitochondrial
level occurred. This study aims to improve existing knowledge necessary for a
correct assessment of the balance between AuNPs potential adverse and beneficial
effects and might have important implications for biomedical applications (e.g.
nanomedicine). To conclude proteomics link to system biology analysis is a valuable
tool to understand and predict nanoparticles' toxicity, furthermore it has the
potential to reveal pathways that may not be immediately evident with classical
toxicological assays. (C) 2014 The Authors. Published by Elsevier Ireland Ltd.
AN - WOS:000336657400006
AU - Gioria, S.
AU - Chassaigne, H.
AU - Carpi, D.
AU - Parracino, A.
AU - Meschini, S.
AU - Barboro, P.
AU - Rossi, F.
DA - JUL 15
DO - 10.1016/j.toxlet.2014.04.016
IS - 2
PY - 2014
SN - 0378-4274
1879-3169
SP - 111-126
ST - A proteomic approach to investigate AuNPs effects in Balb/3T3 cells
TI - A proteomic approach to investigate AuNPs effects in Balb/3T3 cells
VL - 228
ID - 6643
ER -
TY - JOUR
AB - The aims of this study were to evaluate the ratio between inflammatory
reactions induced by four endodontic sealers and the occurrence of fibrosis and the
number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA).
Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus,
Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar
rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8
animals were euthanized, providing 6 specimens per test group and 2 specimens from
the control group. The fragments were subjected to histological processing and
immunohistochemical analysis for anti α-SMA protein. All specimens, except those
from the control group, presented severe inflammatory reaction on the 7th
postoperative day, which also coincided with a large number of myofibroblasts. On
the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill,
AH Plus and Acroseal decreased significantly, which coincided with reduced presence
of myofibroblasts and usual collagen deposition. In contrast, in the group filled
with Epiphany, significant inflammatory cell infiltrate was present in all analyzed
periods. The persistence of an inflammatory reaction induced by endodontic sealer
may also induce the development of fibrosis in combination with presence of
myofibroblasts.
O objetivo deste estudo foi avaliar a relação entre reação inflamatória induzida
por quatro cimentos endodônticos e a presença de fibrose e quantidade de
miofibroblastos que apresentam positividade para α-SMA. Tubos de polietileno foram
preenchidos com o cimento (I: Endofill; II: AH Plus; III: Acroseal; IV: Epiphany) e
inseridos em 4 regiões do dorso de 24 ratos Wistar, enquanto 2 tubos vazios (V -
controle) foram inseridos em 6 ratos. Após 7, 21 e 45 dias, oito animais foram
sacrificados obtendo 6 indivíduos por grupo e 2 para o grupo controle. Os
fragmentos foram submetidos ao processamento histológico e à análise imuno-
histoquímica para a proteína anti-α-SMA. Todos os grupos, exceto o controle,
demonstraram notável reação inflamatória no 7º dia pós-operatório, que também
coincidiu com uma grande quantidade de miofibroblastos. No 21º e 45º dia pós-
operatório, a reação inflamatória induzida pelo Endofill, AH Plus e Acroseal
diminuiu significativamente, o que coincidiu com reduzida presença de
miofibroblastos e deposição de colágeno normal. Em contraste, no grupo Epiphany,
infiltrado inflamatório significativo esteve presente em todos os períodos
analisados. A persistência do infiltrado inflamatório induzido por cimento
endodôntico pode também provocar uma fibrose associada com a presença de
miofibroblastos.
AD - Giovanini, Allan Fernando
Positivo University. Masters Program in Clinical Dentistry. Curitiba. BR
Leonardi, Denise Piotto
Baratto-Filho, Flares
Valença, Paola Cristine
Moresca, Ricardo César
Moro, Alexandre
Schramm, Celso Alfredo
AU - Giovanini, Allan Fernando
AU - Leonardi, Denise Piotto
AU - Baratto-Filho, Flares
AU - Valença, Paola Cristine
AU - Moresca, Ricardo César
AU - Moro, Alexandre
AU - Schramm, Celso Alfredo
C1 - 20111026
DA - 2011/00
DB - LILACS
DO - 10.1590/S0103-64402011000500004
IS - 5
Endodontic sealers
Fibrosis
Myofibroblasts
LA - en
PY - 2011
SN - 0103-6440
SP - 369-376
ST - An endodontic sealer induces a pathological condition when associated with
persistent tissue toxicity and presence of myofibroblasts
T2 - Braz. dent. j
TI - An endodontic sealer induces a pathological condition when associated with
persistent tissue toxicity and presence of myofibroblasts
64402011000500004
VL - 22
ID - 4947
ER -
TY - JOUR
AB - To cellular systems, nanoparticles are considered as foreign particles. Upon
particles and cells contact, innate immune system responds by activating the
inflammatory pathway. However, excessive inflammation had been linked to various
diseases ranging from allergic responses to cancer. Common nanoparticles, namely
silver, titanium dioxide, and zinc oxide exist in the environment as well as in
consumer products at ultralow level of 10(-6)-10(-3) mu g mL(-1). However, so far
the risks of such low NPs concentrations remain unexplored. Therefore, we attempted
to screen the pro-inflammatory responses after ultralow concentration treatments of
the three nanoparticles on RAW264.7 macrophages, which are a part of the immune
system, at both cellular and gene levels. Even though cytotoxicity was only
observed at nanoparticles concentrations as high as 10 mu g mL(-1), through the
level of NF-kappa B and upregulation of pro-inflammatory genes, we observed
activation of the induction of genes encoding pro-inflammatory cytokines starting
already at 10(-7) mu g mL(-1). This calls for more thorough characterization of
nanoparticles in the environment as well as in consumer products to ascertain the
health and safety of the consumers and living systems in general. (C) 2015 Elsevier
B.V. All rights reserved.
AN - WOS:000360595700018
AU - Giovanni, M.
AU - Yue, J. Q.
AU - Zhang, L. F.
AU - Xie, J. P.
AU - Ong, C. N.
AU - Leong, D. T.
DA - OCT 30
DO - 10.1016/j.jhazmat.2015.04.081
PY - 2015
SN - 0304-3894
1873-3336
SP - 146-152
ST - Pro-inflammatory responses of RAW264.7 macrophages when treated with ultralow
concentrations of silver, titanium dioxide, and zinc oxide nanoparticles
T2 - JOURNAL OF HAZARDOUS MATERIALS
TI - Pro-inflammatory responses of RAW264.7 macrophages when treated with ultralow
VL - 297
ID - 6288
ER -
TY - JOUR
AB - To cellular systems, nanoparticles are considered as foreign particles. Upon
particles and cells contact, innate immune system responds by activating the
inflammatory pathway. However, excessive inflammation had been linked to various
diseases ranging from allergic responses to cancer.Commonnanoparticles, namely
silver, titanium dioxide, and zinc oxide exist in the environment as well as in
consumer products at ultralow level of 10-6-10-3 μg mL-1. However, so far the risks
of such low NPs concentrations remain unexplored. Therefore, we attempted to screen
the pro-inflammatory responses after ultralow concentration treatments of the three
nanoparticles on RAW264.7 macrophages, which are a part of the immune system, at
both cellular and gene levels. Even though cytotoxicity was only observed at
nanoparticles concentrations as high as 10μgmL-1, through the level of NF-κB and
upregulation of pro-inflammatory genes, we observed activation of the induction of
genes encoding pro-inflammatory cytokines starting already at 10-7 μg mL-1. This
calls for more thorough characterization of nanoparticles in the environment as
well as in consumer products to ascertain the health and safety of the consumers
and living systems in general. © 2015 Elsevier B.V. All rights reserved.
AU - Giovanni, M.
AU - Yue, J.
AU - Zhang, L.
AU - Xie, J.
AU - Ong, C. N.
AU - Leong, D. T.
DB - Scopus
DO - 10.1016/j.jhazmat.2015.04.081
KW - Nanoparticles
Pro-inflammatory response
RAW264.7
Animals
Cell Line
Cell Survival
Cytokines
Genes, Reporter
Macrophages
Mice
NF-kappa B
Silver
Surface Properties
Titanium
Transfection
Zinc Oxide
Chemical activation
Consumer products
Genes
Immune system
Oxides
Titanium dioxide
Titanium oxides
Zinc
Zinc oxide
cytokine
silver nanoparticle
nanoparticle
silver
titanium
titanium dioxide
zinc oxide
Allergic response
Characterization of nanoparticles
Inflammatory genes
Innate immune systems
bacteriophage
cells and cell components
disease
gene
health and safety
health impact
immune system
oxide
toxicity
zinc
animal cell
Article
controlled study
cytotoxicity
gene induction
inflammation
innate immunity
macrophage
mouse
nonhuman
particle size
RAW 264.7 cell line
upregulation
animal
cell line
cell survival
chemistry
dose response
drug effects
genetics
immunology
reporter gene
surface property
M3 - Article
PY - 2015
SP - 146-152
ST - Pro-inflammatory responses of RAW264.7 macrophages when treated with ultralow
T2 - Journal of Hazardous Materials
TI - Pro-inflammatory responses of RAW264.7 macrophages when treated with ultralow
84966389584&doi=10.1016%2fj.jhazmat.2015.04.081&partnerID=40&md5=4230bf8a6b483c2262
89c7cedd6c1452
VL - 297
ID - 5640
ER -
TY - JOUR
AB - The recent widespread applications of nanomaterials, because of their
properties, opens new scenarios that affect their dispersal in the environment. In
particular multiwall carbon nanotubes (MWCNTs), despite their qualities, seem to be
harmful for animals and humans. To evaluate possible toxic effects caused by carbon
nanotube environmental dispersion, with regard to aquatic compartment, we proposed
as experimental model a freshwater invertebrate: Hirudo medicinalis. In the present
study we analyse acute and chronic immune responses over a short (1, 3, 6 and 12
hours) and long time (from 1 to 5 weeks) exposure to MWCNTs by optical, electron
and immunohistochemical approaches. In the exposed leeches angiogenesis and
fibroplasia accompanied by massive cellular migration occur. Immunocytochemical
characterization using specific markers shows that in these inflammatory processes
the monocyte-macrophages (CD45(+), CD68(+)) are the most involved cells. These
immunocompetent cells are characterized by sequence of events starting from the
expression of pro-inflammatory cytokines (in particular IL-18), and
amyloidogenensis. Our combined experimental approaches, basing on high sensitive
inflammatory response can highlight adverse effects of nanomaterials on aquatic
organisms and could be useful to assess the MWCNTs impact on aquatic, terrestrial
animal and human health.
AN - WOS:000366143800081
AU - Girardello, R.
AU - Tasselli, S.
AU - Baranzini, N.
AU - Valvassori, R.
AU - de Eguileor, M.
AU - Grimaldi, A.
C7 - e0144361
DA - DEC 4
DO - 10.1371/journal.pone.0144361
IS - 12
PY - 2015
SN - 1932-6203
ST - Effects of Carbon Nanotube Environmental Dispersion on an Aquatic
Invertebrate, Hirudo medicinalis
T2 - PLOS ONE
TI - Effects of Carbon Nanotube Environmental Dispersion on an Aquatic
Invertebrate, Hirudo medicinalis
VL - 10
ID - 6783
ER -
TY - JOUR
AB - The aim of the study is to evaluate the therapeutic applications of silver
nanoparticles synthesized using 2 medicinal plants collected from yelagiri hills.
The leaves of the plants were used for optimization of silver nanoparticles by
varying the time exposure of the reaction mixture to sunlight (5, 10, 15 minutes).
The anti-oxidant, anti inflammatory and antimicrobial potentials of samples was
studied by different assays. Also, the synthesized nanoparticles were characterized
by UV, SEM, XRD and FTIR techniques. The results suggest that nanoparticles
synthesis was significant at exposure time of 5 and 10minutes. The synthesized
particles were confirmed by UV spectroscopy, which showed a characteristic peak at
427 and 418nm for the 2 samples respectively. The synthesized nanoparticles were
found to be in the size range of 60-80nm and possessed characteristic XRD peaks.
The results of the study revealed that the synthesized silver nanoparticles
possessed significant antioxidant, anti inflammatory, anti-proliferative and
antimicrobial properties.
AU - Giridharan, T.
AU - Chandran, M.
AU - Sindhu, S.
AU - Arumugam, P.
DB - Scopus
IS - 4
KW - Flacourtia sepiaria
FTIR
MCF7 cells
Rhinacanthus nasutus
SEM
XRD
Flacourtia
ascorbic acid
cefotaxime
doxorubicin
Flacourtia sepiaria extract
free radical
hydroxyl radical
plant extract
Rhinacanthus nasutus extract
silver nanoparticle
tocopherol
unclassified drug
antifungal activity
Article
Bacillus subtilis
cancer cell culture
Candida albicans
Candida tropicalis
controlled study
drug cytotoxicity
Escherichia coli
green synthesis
human
human cell
liver cancer
MCF 7 cell line
medicinal plant
nonhuman
particle size
plant leaf
sun exposure
synthesis
X ray diffraction
M3 - Article
PY - 2014
SP - B560-B569
ST - Comparative studies on green synthesis and therapeutic applications of silver
nano particles using Flacourtia sepiaria and Rhinacanthus nasutus
T2 - International Journal of Pharma and Bio Sciences
TI - Comparative studies on green synthesis and therapeutic applications of silver
nano particles using Flacourtia sepiaria and Rhinacanthus nasutus
84908011906&partnerID=40&md5=a49856f130fd318461bb7b877984d8ae
VL - 5
ID - 5633
ER -
TY - JOUR
AB - The aim of this research was to evaluate the inflammatory and/or oxidative
damage related to silver nanoparticles (AgNPs), which are responsible for negative
effects on sperm physiology and metabolism. Thirty New Zealand White rabbit bucks
were divided into 5 experimental groups (6 animals/group): Control, treated with
0.9% NaCl; AgNP, treated with a 5 mM AgNP solution; LPS, treated with 50 g/kg b.w.
E. coli LPS; AgNPs + NSAID, treated with an anti-inflammatory drug at 0.2 mg/kg
b.w. and 5 mM AgNPs; and AgNPs + Vit E, treated with 0.18 mg/kg b.w. vitamin E and
5 mM AgNPs. Sperm quality and oxidative and inflammatory status were assessed at
different times (0-60 days). Two statistical models were built: the first evaluated
the effects of AgNPs and LPS (vs. Control), whereas the second evaluated the
protective effect of an NSAID and vitamin E against AgNP-induced damage. Three
principal component analyses were performed: sperm traits (motility, volume),
oxidative status (antioxidants, oxidative metabolites, and redox reactions), and
cytokines (TNF-alpha, IL-8, and IL-6). A negative effect on reproductive traits
resulted after NP administration. In particular, an inflammatory/oxidative response
took place in the reproductive tract during the first 2-3 wks of AgNP
administration (cytokine and oxidative metabolite generation); the
inflammatory/oxidative thrust impaired the status of rabbit tissues (seminal
plasma, sperm, and blood), inducing a response (increased antioxidant enzymes and
redox reactions) at 4-7 wks; oxidative stress, if not totally counteracted, likely
induced toxicity in the late phases of AgNP administration (8-9 wks). In
conclusion, exposure to silver nanoparticles produced a similar but more persistent
effect than that of LPS on rabbit reproductive tissues: AgNP administration
triggered a proinflammatory response linked to oxidative thrust, worsening many
sperm parameters. However, only anti-inflammatory treatment counteracted the
negative effects of AgNPs, whereas vitamin E seemed to act as an adjuvant,
attenuating the oxidative cascade.
AN - WOS:000610163600002
AU - Giulia, C.
AU - Simona, M.
AU - Elena, M.
AU - Daniela, C.
AU - Lucia, M.
AU - Ida, F. A.
AU - Laura, M.
AU - Gabriele, B.
AU - Cesare, C.
C7 - 6664062
DA - DEC 28
DO - 10.1155/2020/6664062
PY - 2020
SN - 0962-9351
1466-1861
ST - Oxidative and/or Inflammatory Thrust Induced by Silver Nanoparticles in
T2 - MEDIATORS OF INFLAMMATION
TI - Oxidative and/or Inflammatory Thrust Induced by Silver Nanoparticles in
VL - 2020
ID - 6039
ER -
TY - JOUR
AB - The already known Ag(I)-Sb(III) compound of the formula {Ag(Ph3Sb)3(NO3)} (1)
and two novel mixed metal Ag(I)-Sb(III) metallotherapeutics of the formulae
{Ag(Ph3Sb)3(SalH)}(2) and {Ag(Ph3Sb)3(Asp)}(3) (SalH2 = salicylic acid, AspH =
aspirin or 2-acetylsalicylic acid and Ph3Sb = triphenyl antimony(III)) have been
synthesised and characterised by m.p., vibrational spectroscopy (mid-FT-IR),
13C-,1H-NMR, UV-visible (UV-vis) spectroscopic techniques, high resolution mass
spectroscopy (HRMS) and X-ray crystallography. Compounds 1,- 3 were treated with
the surfactant cetyltrimethylammonium bromide (CTAB) in order to enhance their
solubility and as a consequence their bioactivity. The resulting micelles a-c were
characterised with X-ray powder diffraction (XRPD) analysis, X-ray fluorescence
(XRF) spectroscopy, Energy-dispersive X-ray spectroscopy (EDX), conductivity,
Thermal gravimetry-differential thermal analysis (TG-DTA), and atomic absorption.
Compounds 1-3 and the relevant micelles a-c were evaluated for their in vitro
cytotoxic activity against human cancer cell lines: MCF-7 (breast, estrogen
receptor (ER) positive), MDA-MB-231 (breast, ER negative) and MRC-5 (normal human
fetal lung fibroblast cells) with sulforhodamine B (SRB) colorimetric assay. The
results show significant increase in the activity of micelles compared to that of
the initial compounds. Moreover, micelles exhibited lower activity against normal
cells than tumor cells. The binding affinity of a-c towards the calf thymus (CT)-
DNA, lipoxygenase (LOX) and glutathione (GSH) was studied by the fluorescent
emission light and UV-vis spectroscopy. © 2015 Elsevier Inc.
AU - Gkaniatsou, E. I.
AU - Banti, C. N.
AU - Skoulika, S.
AU - Manoli, M.
DB - Scopus
DO - 10.1016/j.jinorgbio.2015.04.014
KW - Anti-inflammatory drugs
Bioinorganic chemistry
Cytotoxic activity
Micelles
Silver(I)-antimony(III) compounds
Antimony
Aspirin
Cetrimonium Compounds
Glutathione
Humans
Kinetics
Linoleic Acid
Lipoxygenase
MCF-7 Cells
Proton Magnetic Resonance Spectroscopy
Receptors, Estrogen
Salicylic Acid
Silver
Solubility
Surface-Active Agents
antimony
cetrimide
glutathione
lipoxygenase
salicylic acid
silver
sulforhodamine B
estrogen receptor
linoleic acid
micelle
surfactant
Article
atomic absorption
binding affinity
biological activity
calf thymus
carbon nuclear magnetic resonance
colorimetry
conductance
cytotoxicity
differential scanning calorimetry
drug solubility
energy dispersive x ray spectroscopy
fetus lung
fluorescence spectroscopy
high resolution mass spectroscopy
human
human cell
in vitro study
lung fibroblast
mass spectrometry
MCF 7 cell line
roentgen spectroscopy
thermal gravimetry differential thermal analysis
thymus
X ray fluorescence
chemistry
kinetics
metabolism
solubility
spectrometry
synthesis
M3 - Article
PY - 2015
SP - 108-119
ST - Novel mixed metal Ag(I)-Sb(III)-metallotherapeutics of the NSAIDs, aspirin
and salicylic acid: Enhancement of their solubility and bioactivity by using the
surfactant CTAB
TI - Novel mixed metal Ag(I)-Sb(III)-metallotherapeutics of the NSAIDs, aspirin
and salicylic acid: Enhancement of their solubility and bioactivity by using the
surfactant CTAB
84948712625&doi=10.1016%2fj.jinorgbio.2015.04.014&partnerID=40&md5=bc5564aed4251a14
d0f27eb42c4c6113
VL - 150
ID - 5607
ER -
TY - JOUR
AB - Silver (Ag) nanoparticles are commonly used in consumer products due to their
antimicrobial properties. Here we studied the impact of Ag nanoparticles on immune
responses by using cell lines of monocyte/macrophage and lung epithelial cell
origin, respectively. Short-term experiments (24 h) showed that Ag nanoparticles
reduced the lipopolysaccharide (LPS)-induced secretion of pro-inflammatory
cytokines in THP-1 cells under serum-free conditions. ICP-MS analysis revealed that
cellular uptake of Ag was higher under these conditions. Long-term exposure (up to
6 weeks) of BEAS-2B cells to Ag nanoparticles also suppressed pro-inflammatory
cytokine production following a brief challenge with LPS. Experiments using
reporter cells revealed that Ag nanoparticles as well as AgNO3 inhibited LPS-
triggered Toll-like receptor (TLR) signaling. Furthermore, RNA-sequencing of BEAS-
2B cells indicated that Ag nanoparticles affected TLR signaling pathways. In
conclusion, Ag nanoparticles reduced the secretion of pro-inflammatory cytokines in
response to LPS, likely as a result of the release of silver ions leading to an
interference with TLR signaling. This could have implications for the use of Ag
nanoparticles as antibacterial agents. Further in vivo studies are warranted to
study this.
AN - WOS:000517128400008
AU - Gliga, A. R.
AU - De Loma, J.
AU - Di Bucchianico, S.
AU - Skoglund, S.
AU - Keshavan, S.
AU - Wallinder, I. O.
AU - Karlsson, H. L.
AU - Fadeel, B.
DA - FEB 1
DO - 10.1039/c9na00721k
IS - 2
PY - 2020
SN - 2516-0230
SP - 648-658
ST - Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor
signaling in immune-competent human cell lines
T2 - NANOSCALE ADVANCES
TI - Silver nanoparticles modulate lipopolysaccharide-triggered Toll-like receptor
signaling in immune-competent human cell lines
VL - 2
ID - 6064
ER -
TY - JOUR
AB - Nanotechnology occupies a prominent space in economy and science due to the
beneficial properties of nanomaterials. However, nanoparticles may pose risks to
living organisms due to their adsorption and pro-oxidative properties. The aim of
the current study was to investigate the effects of polymer-coated silver
nanoparticles (AgNPs) and organochlorine pesticides (OCPs), as well as their
combined effects on mouse peritoneal macrophages. Macrophages were isolated and
exposed to three concentrations of AgNPs (groups: N1 = 30, N2 = 300 and N3 = 3000
ng.ml(-1)), two concentrations of OCPs (groups: P1 = 30 and P2 = 300 ng.ml(-1)) and
the six possible combinations of these two contaminants for 24 h. AgNPs had
irregular shape, Feret diameter of 8.7 +/- 7.5 nm and zeta potential of -28.7 +/-
3.9 mV in water and -10.7 +/- 1.04 mV in culture medium. OCP mixtures and the lower
concentrations of AgNPs had no detectable effects on cell parameters, but the
highest AgNPs concentration showed high toxicity (trypan blue and MTT assays)
resulting in morphological changes, increase of nitric oxide levels and phagocytic
index. Foremost, the association of N3 and P2 led to distinct effects from those
observed under single exposure.
AN - WOS:000380052700004
AU - Glinski, A.
AU - Liebel, S.
AU - Pelletier, E.
AU - Voigt, C. L.
AU - Randi, M. A. F.
AU - Campos, S. X.
AU - Ribeiro, C. A. O.
AU - Neto, F. F.
DO - 10.3109/15376516.2016.1159770
IS - 4
PY - 2016
SN - 1537-6516
1537-6524
SP - 251-259
ST - Toxicological interactions of silver nanoparticles and organochlorine
pesticides in mouse peritoneal macrophages
T2 - TOXICOLOGY MECHANISMS AND METHODS
TI - Toxicological interactions of silver nanoparticles and organochlorine
pesticides in mouse peritoneal macrophages
VL - 26
ID - 5977
ER -
TY - JOUR
AB - Consumer products manufactured with antimicrobial silver nanoparticles
(AgNPs) may affect the gastrointestinal (GI) system. The human GI-tract is complex
and there are physiological and anatomical differences between human and animal
models that limit comparisons between species. Thus, assessment of AgNP toxicity on
the human GI-tract may require tools that allow for the examination of subtle
changes in inflammatory markers and indicators of epithelial perturbation. Fresh
tissues were excised from the GI-tract of human male and female subjects to
evaluate the effects of AgNPs on the GI-system. The purpose of this study was to
perform an assessment on the ability of the ex vivo model to evaluate changes in
levels of pro-/anti-inflammatory cytokines/chemokines and mRNA expression of
intestinal permeability related genes induced by AgNPs in ileal tissues. The ex
vivo model preserved the structural and biological functions of the in-situ organ.
Analysis of cytokine expression data indicated that intestinal tissue of male and
female subjects responded differently to AgNP treatment, with male samples showing
significantly elevated Granulocyte-macrophage colonystimulating factor (GM-CSF)
after treatment with 10 nm and 20 nm AgNPs for 2 h and significantly elevated
RANTES after treatment with 20 nm AgNPs for 24 h. In contrast, tissues of female
showed no significant effects of AgNP treatment at 2 h and significantly decreased
RANTES (20 nm), TNF-α (10 nm), and IFN-γ (10 nm) at 24 h. Smaller size AgNPs (10
nm) perturbed more permeability-related genes in samples of male subjects, than in
samples from female subjects. In contrast, exposure to 20 nm AgNPs resulted in
upregulation of a greater number of genes in female-derived samples (36 genes) than
in male-derived samples (8 genes). The ex vivo tissue model can distinguish sex
dependent effects of AgNP and could serve as a translational non-animal model to
assess the impacts of xenobiotics on human intestinal mucosa. © 2020 by the
AU - Gokulan, K.
AU - Williams, K.
AU - Orr, S.
AU - Khare, S.
C7 - 9
DB - Scopus
DO - 10.3390/ijms22010009
IS - 1
KW - Ex vivo intestinal model
Human intestine
Immune status
Intestinal mucosa
Permeability
Cytokines
Epithelial Cells
Female
Humans
Ileum
Intestinal Mucosa
Male
Metal Nanoparticles
Microscopy, Electron, Scanning
Particle Size
RNA, Messenger
Sex Factors
Silver
caveolin 1
gamma interferon
granulocyte macrophage colony stimulating factor receptor
interleukin 10
interleukin 2
interleukin 6
interleukin 8
messenger RNA
RANTES
RNA
silver nanoparticle
autacoid
cytokine
metal nanoparticle
silver
adherens junction
Article
controlled study
desmosome
epithelium cell
ex vivo study
explant
exposure
female
gene expression
human
human tissue
ileum mucosa
immune response
inflammation
intestine tissue
limit of detection
male
protein expression
sex difference
tight junction
upregulation
drug effect
genetics
ileum
intestine mucosa
metabolism
particle size
permeability
sex factor
ultrastructure
M3 - Article
PY - 2021
SP - 1-15
ST - Human intestinal tissue explant exposure to silver nanoparticles reveals sex
dependent alterations in inflammatory responses and epithelial cell permeability
TI - Human intestinal tissue explant exposure to silver nanoparticles reveals sex
dependent alterations in inflammatory responses and epithelial cell permeability
85098484265&doi=10.3390%2fijms22010009&partnerID=40&md5=ad7f106f8df9f2e0b8c5ffb252b
6cb40
VL - 22
ID - 5293
ER -
TY - JOUR
AB - Systemic exposure to nanoparticles (NPs) adversely affects different organs,
including the nervous system. We systematically extracted data from publication on
PubMed and Embase database up to the year 2020, and analyzed in vitro and in vivo
neurotoxicity of 4 of the most well studied NPs (silver NPs, carbon-based NPs, iron
NPs and silica NPs). A relatively good correlation was observed between in vitro
and in vivo effects, including genotoxicity, oxidative stress, apoptosis and pro-
inflammatory effects. However, crucial knowledge gap exists in current
understanding of the underlying mechanisms. Some of the critical knowledge gaps and
research needs identified in relation to neurotoxicity of nanoparticles include (1)
lack of physio-chemical characteristics of NPs used, (2) cellular/tissue uptake of
NP, (3) NP translocation across the blood-brain barrier (BBB), (4) Effect of
exposure routes. © 2022, The Author(s), under exclusive licence to Springer-Verlag
GmbH Germany, part of Springer Nature.
AU - Gong, J. Y.
AU - Holt, M. G.
AU - Hoet, P. H. M.
AU - Ghosh, M.
DB - Scopus
DO - 10.1007/s00204-022-03233-1
IS - 5
KW - Blood-Brain Barrier
Humans
Metal Nanoparticles
Nanoparticles
Oxidative Stress
Silicon Dioxide
carbon nanoparticle
iron oxide nanoparticle
nanoparticle
silica nanoparticle
silver nanoparticle
metal nanoparticle
silicon dioxide
apoptosis
astrocyte
blood brain barrier
cell activation
cell death
cell transport
cell ultrastructure
cytotoxicity
Embase
exposure
genotoxicity
histopathology
human
in vitro study
in vivo study
inflammation
Medline
microglia
neurotoxicity
nonhuman
oxidative stress
Review
systematic review
M3 - Review
PY - 2022
SP - 1141-1212
ST - Neurotoxicity of four frequently used nanoparticles: a systematic review to
reveal the missing data
TI - Neurotoxicity of four frequently used nanoparticles: a systematic review to
reveal the missing data
85126117209&doi=10.1007%2fs00204-022-03233-
1&partnerID=40&md5=2f92a150d969d1fc125e256356877cf9
VL - 96
ID - 5086
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are known to penetrate into the brain and cause
neuronal death. However, there is a paucity in studies examining the effect of AgNP
on the resident immune cells of the brain, microglia. Given microglia are
implicated in neurodegenerative disorders such as Parkinson's disease (PD), it is
important to examine how AgNPs affect microglial inflammation to fully assess AgNP
neurotoxicity. In addition, understanding AgNP processing by microglia will allow
better prediction of their long term bioreactivity. In the present study, the in
vitro uptake and intracellular transformation of citrate-capped AgNPs by microglia,
as well as their effects on microglial inflammation and related neurotoxicity were
examined. Analytical microscopy demonstrated internalization and dissolution of
AgNPs within microglia and formation of non-reactive silver sulphide (Ag2S) on the
surface of AgNPs. Furthermore, AgNP-treatment up-regulated microglial expression of
the hydrogen sulphide (H2S)-synthesizing enzyme cystathionine-3-lyase (CSE). In
addition, AgNPs showed significant anti-inflammatory effects, reducing
lipopolysaccharide (LPS)-stimulated ROS, nitric oxide and TNFα production, which
translated into reduced microglial toxicity towards dopaminergic neurons. Hence,
the present results indicate that intracellular Ag2S formation, resulting from CSE-
mediated H2S production in microglia, sequesters Ag+ ions released from AgNPs,
significantly limiting their toxicity, concomitantly reducing microglial
inflammation and related neurotoxicity. © 2017 The Author(s).
AU - Gonzalez-Carter, D. A.
AU - Leo, B. F.
AU - Ruenraroengsak, P.
AU - Chen, S.
AU - Goode, A. E.
AU - Theodorou, I. G.
AU - Chung, K. F.
AU - Carzaniga, R.
AU - Shaffer, M. S. P.
AU - Dexter, D. T.
AU - Ryan, M. P.
AU - Porter, A. E.
C7 - 42871
DB - Scopus
DO - 10.1038/srep42871
KW - Animals
Cell Line
Cell Survival
Cystathionine gamma-Lyase
Encephalitis
Hydrogen Sulfide
Lipopolysaccharides
Metal Nanoparticles
Mice
Microglia
Models, Biological
Neurons
Oxidative Stress
Silver
cystathionine gamma lyase
hydrogen sulfide
lipopolysaccharide
metal nanoparticle
silver
animal
biological model
cell line
cell survival
chemistry
cytology
drug effect
encephalitis
metabolism
microglia
mouse
nerve cell
oxidative stress
M3 - Article
PY - 2017
ST - Silver nanoparticles reduce brain inflammation and related neurotoxicity
through induction of H 2 S-synthesizing enzymes
TI - Silver nanoparticles reduce brain inflammation and related neurotoxicity
through induction of H 2 S-synthesizing enzymes
85014466450&doi=10.1038%2fsrep42871&partnerID=40&md5=49685fba68da7a3ad8f79ffbffe5fe
bf
VL - 7
ID - 5531
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are known to penetrate into the brain and cause
neuronal death. However, there is a paucity in studies examining the effect of AgNP
on the resident immune cells of the brain, microglia. Given microglia are
implicated in neurodegenerative disorders such as Parkinson's disease (PD), it is
important to examine how AgNPs affect microglial inflammation to fully assess AgNP
neurotoxicity. In addition, understanding AgNP processing by microglia will allow
better prediction of their long term bioreactivity. In the present study, the in
vitro uptake and intracellular transformation of citrate-capped AgNPs by microglia,
as well as their effects on microglial inflammation and related neurotoxicity were
examined. Analytical microscopy demonstrated internalization and dissolution of
AgNPs within microglia and formation of non-reactive silver sulphide (Ag2S) on the
surface of AgNPs. Furthermore, AgNP-treatment up-regulated microglial expression of
the hydrogen sulphide (H2S)-synthesizing enzyme cystathionine-gamma-lyase (CSE). In
addition, AgNPs showed significant anti-inflammatory effects, reducing
lipopolysaccharide (LPS)-stimulated ROS, nitric oxide and TNF alpha production,
which translated into reduced microglial toxicity towards dopaminergic neurons.
Hence, the present results indicate that intracellular Ag2S formation, resulting
from CSE-mediated H2S production in microglia, sequesters Ag+ ions released from
AgNPs, significantly limiting their toxicity, concomitantly reducing microglial
inflammation and related neurotoxicity.
AN - WOS:000395369700001
AU - Gonzalez-Carter, D. A.
AU - Leo, B. F.
AU - Chen, S.
AU - Goode, A. E.
AU - Chung, K. F.
AU - Carzaniga, R.
AU - Dexter, D. T.
AU - Ryan, M. P.
AU - Porter, A. E.
C7 - 42871
DA - MAR 2
DO - 10.1038/srep42871
PY - 2017
SN - 2045-2322
ST - Silver nanoparticles reduce brain inflammation and related neurotoxicity
through induction of H2S-synthesizing enzymes
TI - Silver nanoparticles reduce brain inflammation and related neurotoxicity
through induction of H2S-synthesizing enzymes
VL - 7
ID - 6104
ER -
TY - JOUR
AB - The aim of this study was to determine the effect of AgNPs on the epigenome
of endothelial cells EA.hy926, including the levels of expression of microRNAs
(miRNAs) and global DNA methylation patterns. In addition, evaluation of the
expression of inflammatory genes and the levels of VCAM-1 protein (miRNA-126
target) was performed. The expression levels of analyzed miRNAs (microRNAs-126, 155
and 146) were reduced significantly and there were not observed changes in
inflammatory gene expression. Regarding the levels of protein vascular cell
adhesion molecule 1 (VCAM-1), they increase significantly to 0.5 mu M AgNPs at 24 h
of exposure. As far as DNA methylation is concerned, we found that AgNPs induce a
state of global hyper-methylation. In conclusion, it was demonstrated that direct
contact between AgNPs and endothelial cells resulted in the dysregulation of highly
enriched and vastly functional miRNAs and DNA hypermethylation, that may have
multiple effects on endothelium function and integrity.
AN - WOS:000606755900003
AU - Gonzalez-Palomo, A. K.
AU - Saldana-Villanueva, K.
AU - Cortes-Garcia, J. D.
AU - Fernandez-Macias, J. C.
AU - Mendez-Rodriguez, K. B.
AU - Maldonado, I. N. P.
C7 - 103543
DA - JAN
DO - 10.1016/j.etap.2020.103543
PY - 2021
SN - 1382-6689
1872-7077
ST - Effect of silver nanoparticles (AgNPs) exposure on microRNA expression and
global DNA methylation in endothelial cells EA.hy926
T2 - ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
TI - Effect of silver nanoparticles (AgNPs) exposure on microRNA expression and
VL - 81
ID - 6237
ER -
TY - JOUR
AB - The aim of this study was to determine the effect of AgNPs on the epigenome
of endothelial cells EA.hy926, including the levels of expression of microRNAs
(miRNAs) and global DNA methylation patterns. In addition, evaluation of the
expression of inflammatory genes and the levels of VCAM-1 protein (miRNA-126
target) was performed. The expression levels of analyzed miRNAs (microRNAs-126, 155
and 146) were reduced significantly and there were not observed changes in
inflammatory gene expression. Regarding the levels of protein vascular cell
adhesion molecule 1 (VCAM-1), they increase significantly to 0.5 μM AgNPs at 24 h
of exposure. As far as DNA methylation is concerned, we found that AgNPs induce a
state of global hyper-methylation. In conclusion, it was demonstrated that direct
contact between AgNPs and endothelial cells resulted in the dysregulation of highly
enriched and vastly functional miRNAs and DNA hypermethylation, that may have
multiple effects on endothelium function and integrity. © 2020
AU - González-Palomo, A. K.
AU - Saldaña-Villanueva, K.
AU - Cortés-García, J. D.
AU - Fernández-Macias, J. C.
AU - Méndez-Rodríguez, K. B.
AU - Pérez Maldonado, I. N.
C7 - 103543
DB - Scopus
DO - 10.1016/j.etap.2020.103543
KW - AgNPs
Citotoxicity
DNA methylation
Endothelial cells
microRNAs
Nanomaterials
Cell Line
DNA Methylation
Endothelial Cells
Gene Expression
Humans
Metal Nanoparticles
MicroRNAs
Silver
Vascular Cell Adhesion Molecule-1
DNA
microRNA
microRNA 126
microRNA 146
microRNA 155
silver nanoparticle
unclassified drug
metal nanoparticle
silver
Article
cell culture
cell viability
controlled study
cytotoxicity
EA.hy 926 cell line
epigenetics
epigenome
gene expression
gene expression level
gene function
human
human cell
in vitro study
nanotoxicology
priority journal
cell line
drug effect
endothelium cell
metabolism
M3 - Article
PY - 2021
ST - Effect of silver nanoparticles (AgNPs) exposure on microRNA expression and
TI - Effect of silver nanoparticles (AgNPs) exposure on microRNA expression and
85096232622&doi=10.1016%2fj.etap.2020.103543&partnerID=40&md5=4fe47e79feb3547217f37
8c63dd674e3
VL - 81
ID - 5296
ER -
TY - JOUR
AB - Comparative hazard identification of nanomaterials (NMs) can aid in the
prioritisation for further toxicity testing. Here, we assessed the acute lung,
systemic and liver responses in C57BL/6N mice for three NMs to provide a hazard
ranking. A silver (Ag), non-functionalised zinc oxide (ZnO) and a
triethoxycaprylylsilane functionalised ZnO NM suspended in water with 2% mouse
serum were examined 24 hours following a single intratracheal instillation (IT.).
An acute pulmonary inflammation was noted (marked by a polymorphonuclear neutrophil
influx) with cell damage (LDH and total protein) in broncho-alveolar lavage fluid
(BALF) after administration of both non-functionalised and functionalised ZnO. The
latter also induced systemic inflammation measured as an increase in blood
neutrophils and a decrease in blood lymphocytes. Exposure to Ag NM was not
accompanied by pulmonary inflammation or cytotoxicity, or by systemic inflammation.
A decrease in glutathione levels was demonstrated in the liver following exposure
to high doses of all three nanomaterials irrespective of any noticeable
inflammatory or cytotoxic effects in the lung. By applying benchmark dose (BMD)
modeling statistics to compare potencies of the NMs, we rank functionalised ZnO
ranked the highest based on the largest number of affected endpoints, as well as
the strongest responses observed after 24 hours. The non-functionalised ZnO NM gave
an almost similar response, whereas Ag NM did not cause an acute response at
similar doses. © 2015 Gosens et al.
AU - Gosens, I.
AU - Lenz, A. G.
AU - Bokkers, B.
AU - De Jong, W. H.
AU - Krystek, P.
AU - Tran, L.
AU - Stone, V.
AU - Wallin, H.
AU - Stoeger, T.
AU - Cassee, F. R.
C7 - 0126934
DB - Scopus
DO - 10.1371/journal.pone.0126934
IS - 5
KW - Animals
Injections, Spinal
Instillation, Drug
Lung
Mice
Mice, Inbred C57BL
Nanostructures
Silver
Toxicity Tests
Zinc Oxide
Mus
glutathione
protein
silane derivative
silver
silver nanoparticle
triethoxycaprylylsilane
unclassified drug
water
zinc oxide
nanomaterial
animal cell
animal experiment
animal tissue
Article
C57BL/6N mouse
cell damage
comparative hazard identification
controlled study
cytotoxicity
exposure
female
hazard assessment
inflammation
liver
lung
lung lavage
lymphocyte
mouse
nonhuman
statistical model
toxicity testing
animal
C57BL mouse
chemistry
comparative study
drug effects
drug instillation
intraspinal drug administration
metabolism
pathology
procedures
M3 - Article
PY - 2015
ST - Comparative hazard identification by a single dose lung exposure of zinc
oxide and silver nanomaterials in mice
T2 - PLoS ONE
TI - Comparative hazard identification by a single dose lung exposure of zinc
oxide and silver nanomaterials in mice
84930678084&doi=10.1371%2fjournal.pone.0126934&partnerID=40&md5=c9844120208678e8a2f
849b3ab41a919
VL - 10
ID - 5627
ER -
TY - JOUR
AB - BackgroundMonocytes are myeloid cells that reside in the blood and bone
marrow and respond to inflammation. At the site of inflammation, monocytes express
cytokines and chemokines. Monocytes have been shown to be cytotoxic to tumor cells
in the presence of pro-inflammatory cytokines such as Interferon Alpha, Interferon
Gamma, and IL-6. We have previously shown that monocytes stimulated with both
interferons (IFNs) results in synergistic killing of ovarian cancer cells. We
translated these observations to an ongoing clinical trial using adoptive cell
transfer of autologous monocytes stimulated ex vivo with IFNs and infused into the
peritoneal cavity of patients with advanced, chemotherapy resistant, ovarian
cancer. Here we describe the optimization of the monocyte elutriation protocol and
a cryopreservation protocol of the monocytes isolated from peripheral
blood.MethodsCounter flow elutriation was performed on healthy donors or women with
ovarian cancer. The monocyte-containing, RO-fraction was assessed for total
monocyte number, purity, viability, and cytotoxicity with and without a
cryopreservation step. All five fractions obtained from the elutriation procedure
were also assessed by flow cytometry to measure the percent of immune cell subsets
in each fraction.ResultsBoth iterative monocyte isolation using counter flow
elutriation or cryopreservation following counter flow elutriation can yield over 2
billion monocytes for each donor with high purity. We also show that the monocytes
are stable, viable, and retain cytotoxic functions when cultured with
IFNs.ConclusionLarge scale isolation of monocytes from both healthy donors and
patients with advanced, chemotherapy resistant ovarian cancer, can be achieved with
high total number of monocytes. These monocytes can be cryopreserved and maintain
viability and cytotoxic function. All of the elutriated cell fractions contain
ample immune cells which could be used for other cell therapy-based applications.
AN - WOS:000461327900003
AU - Green, D. S.
AU - Nunes, A. T.
AU - Tosh, K. W.
AU - David-Ocampo, V.
AU - Fellowes, V. S.
AU - Ren, J. Q.
AU - Jin, J. J.
AU - Frodigh, S. E.
AU - Pham, C.
AU - Procter, J.
AU - Tran, C.
AU - Ekwede, I.
AU - Khuu, H.
AU - Stroncek, D. F.
AU - Highfill, S. L.
AU - Zoon, K. C.
AU - Annunziata, C. M.
C7 - 82
DA - MAR 14
DO - 10.1186/s12967-019-1822-6
PY - 2019
SN - 1479-5876
ST - Production of a cellular product consisting of monocytes stimulated with
Sylatron((R)) (Peginterferon alfa-2b) and Actimmune((R)) (Interferon gamma-1b) for
human use
T2 - JOURNAL OF TRANSLATIONAL MEDICINE
TI - Production of a cellular product consisting of monocytes stimulated with
Sylatron((R)) (Peginterferon alfa-2b) and Actimmune((R)) (Interferon gamma-1b) for
human use
VL - 17
ID - 6745
ER -
TY - JOUR
AB - Highly refined mineral hydrocarbons (MHCs) such as low melting point paraffin
wax (LMPW) and low viscosity white oils can cause inflammatory changes in the liver
and mesenteric lymph nodes (MLNs) of the Fischer-344 (F-344) rat. In contrast, only
minimal MLN changes are seen in the Sprague-Dawley (S-D) rat with no changes in the
liver. In this study, the response of female F-344 and S-D rats was compared after
90 days dietary treatment with 0%. 0.2% or 2% LMPW. Effects in the F-344 rats were
significantly greater than in the S-D rats: increased liver and splenic weights and
inflammatory changes (hepatic microgranulomas) in these tissues were observed only
in the F-344 rats. Microgranulomas in the MLNs were observed in both strains but
the effects were substantially greater in the F-344 rats. Cellular markers of
inflammation were examined in a subset of rats from each group using
immunohistochemical staining. An increase in staining for CD3 (T-cells), CD8a
(suppresser/cytotoxic T-cells) and CD4 (helper T-cells) correlated with an increase
in lymphoid cells in the livers of treated F-344 rats. The majority of macrophages
in the hepatic microgranulomas of treated F-344 rats were negative for the ED2
marker, indicating a likely origin from non-resident macrophages. Electron
microscopy showed Kupffer cell hypertrophy and hyperplasia in treated F-344 rats.
However, lysozyme staining (indicating activation of epithelioid macrophages)
decreased with increasing granuloma size. Non-ED2 expressing cells may have been
recruited but not sufficiently activated to be lysozyme positive. Inflammatory
changes in the cardiac mitral valve noted in previous studies of LMPW were also
seen in the F-344 rats in this study but not in the S-D rats. Chemical analysis
showed that MHC accumulated in livers from treated F-344 but not S-D rats and the
concentration was more than 2-fold greater in MLNs from the F-344 than from the S-D
rats. The F-344 appears to be more immunologically sensitive to a number of agents
than other rat strains and the results of this study suggest that this may
contribute, along with pharmacokinetic differences, to the inflammatory response of
F-344 rats to dietary MHCs. (C) 2009 Elsevier Ltd. All rights reserved.
AN - WOS:000274460400054
AU - Griffis, L. C.
AU - Twerdok, L. E.
AU - Francke-Carroll, S.
AU - Biles, R. W.
AU - Schroeder, R. E.
AU - Bolte, H.
AU - Faust, H.
AU - Hall, W. C.
AU - Rojko, J.
DA - JAN
DO - 10.1016/j.fct.2009.10.024
IS - 1
PY - 2010
SN - 0278-6915
SP - 363-372
ST - Comparative 90-day dietary study of paraffin wax in Fischer-344 and Sprague-
Dawley rats
T2 - FOOD AND CHEMICAL TOXICOLOGY
TI - Comparative 90-day dietary study of paraffin wax in Fischer-344 and Sprague-
Dawley rats
VL - 48
ID - 6712
ER -
TY - JOUR
AB - The inhalation toxicology of ricin (supplied by Sigma) from the seed variety
"Hale Queen" and abrin was examined following head-only exposure of rats to a range
of concentrations of each toxin generated as an aerosol from solution using a
constant-output nebulizer. The inhalation toxicity of an in-house preparation of
ricin from a different seed type, Ricinus communis var. zanzibariensis (R.
zanzibariensis), was also assessed for comparison. The approximate LCt50 values
determined were very similar for the Sigma ricin and abrin (4.54-5.96 and 4.54 mg
min m-3, respectively). However, the LCt50 of ricin toxin prepared in-house from
seeds of the R. zanzibariensis variety was assessed to be 12.7 mg min m-3. Ricin
prepared from this seed variety was therefore less toxic than Sigma ricin by a
factor of almost threefold. Given that both ricin preparations were pure by silver-
stained, sodium dodecyl sulfate polyacrylamide electrophoresis gels, the data must
reflect differences in specific toxicity between seed varieties. The histopathology
was studied in a separate group of experimental animals exposed to approximate
LCt30 levels of each in-house toxin preparation and was found to be entirely
restricted to the lung. The overall pattern and time course of damage observed were
similar for ricin and abrin and were characterized by rapidly progressive and
overwhelming pulmonary edema accompanied by acute destructive alveolitis and
necrosis/apoptosis of the lower respiratory tract epithelium; severe intraalveolar
edema and resulting hypoxia accounted for the majority of deaths in the decedent
population. The resolution phase of the pulmonary damage in those animals destined
to survive was heralded by a gradual disappearance of edema fluid accompanied by
generalized, focally florid, hyperplasia of type II pneumocytes and striking
transitory consolidation of the lung parenchyma by chronic inflammatory cells.
Despite many similarities in histopathology between abrin and ricin there were some
differences. Although by systemic administration abrin is several times more toxic
than ricin, when delivered by inhalation there was no significant difference in
potency between abrin and the commercial preparation of ricin (Sigma). © 1995
Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
AU - Griffiths, G. D.
AU - Rice, P.
AU - Allenby, A. C.
AU - Bailey, S. C.
AU - Upshall, D. G.
DB - Scopus
DO - 10.3109/08958379509029098
IS - 2
M3 - Article
PY - 1995
SP - 269-288
ST - Inhalation toxicology and histopathology of ricin and abrin toxins
T2 - Inhalation Toxicology
TI - Inhalation toxicology and histopathology of ricin and abrin toxins
0000876903&doi=10.3109%2f08958379509029098&partnerID=40&md5=7ae661ae7db2c2dd437c5ef
23ac2cc67
VL - 7
ID - 5828
ER -
TY - JOUR
AB - Polyethylene terephthalate (PET) is a major pollutant polymer, due to its
wide use in food packaging and fiber production industries worldwide. Currently,
there is great interest for recycling the huge amount of PET-based materials,
derived especially from the food and textile industries. In this study, we applied
the electrospinning technique to obtain nanostructured fibrillary membranes based
on PET materials. Subsequently, the recycled PET networks were decorated with
silver nanoparticles through the chemical reduction method for antimicrobial
applications. After the characterization of the materials in terms of
crystallinity, chemical bonding, and morphology, the effect against Gram-positive
and Gram-negative bacteria, as well as fungal strains, was investigated.
Furthermore, in vitro and in vivo biocompatibility tests were performed in order to
open up potential biomedical applications, such as wound dressings or implant
coatings. Silver-decorated fibers showed lower cytotoxicity and inflammatory
effects and increased antibiofilm activity, thus highlighting the potential of
these systems for antimicrobial purposes. © 2019 by the authors. Licensee �MDPI,
Basel, Switzerland.
AU - Stoica, A. E.
AU - Dima-Bălcescu, M. Ș
AU - Chircov, C.
AU - Gharbia, S.
AU - Baltă, C.
AU - Roșu, M.
AU - Herman, H.
AU - Holban, A. M.
AU - Ficai, A.
AU - Vasile, B. S.
AU - Andronescu, E.
AU - Chifiriuc, M. C.
AU - Hermenean, A.
C7 - 1039
DB - Scopus
DO - 10.3390/jcm8071039
IS - 7
Biocompatibility
Electrospinning
Nanofibers
PET
Polyethylene terephthalate
C reactive protein
nanofiber
polybutylene terephthalate
silver nanoparticle
amniotic fluid stem cell
animal experiment
animal model
animal tissue
anti-infective therapy
antifungal activity
Article
bacterial strain
biocompatibility
biofilm
biological activity
blood sampling
chemical binding
chemical reaction
controlled study
cytotoxicity
electrospinning
fungal strain
histopathology
human
human cell
mouse
MTT assay
nonhuman
particle size
phagocytosis
plankton
X ray diffraction
M3 - Article
PY - 2019
ST - Electrospun polyethylene terephthalate nanofibers loaded with silver
nanoparticles: Novel approach in anti-infective therapy
T2 - Journal of Clinical Medicine
TI - Electrospun polyethylene terephthalate nanofibers loaded with silver
nanoparticles: Novel approach in anti-infective therapy
85076947131&doi=10.3390%2fjcm8071039&partnerID=40&md5=062acb5060622c269804d1f587ef8
be3
VL - 8
ID - 5435
ER -
TY - JOUR
AB - Responsive, theranostic nanosystems, capable of both signaling and treating
wound infections, is a sophisticated approach to reduce the most common and
potentially traumatizing side effects of burn wound treatment: slowed wound healing
due to prophylactic anti-infective drug exposure as well as frequent painful
dressing changes. Antimicrobials as well as dye molecules have been incorporated
into biodegradable nanosystems that release their content only in the presence of
pathogens. Following nanocarrier degradation by bacterial enzymes, any infection
will thus emit a visible signal and be effectively treated at its source. In this
study, we investigated the effect of fluorescent-labeled hyaluronan nanocapsules
containing polyhexanide biguanide and poly-L-lactic acid nanoparticles loaded with
octenidine on primary human dermal microvascular endothelial cells, which play a
major role in cutaneous wound healing. Microscopic and flow cytometric analysis
indicated a time-dependent uptake of both the nanocapsules and the nanoparticles.
However, enzyme immunoassays showed no significant influence on the expression of
pro-inflammatory cell adhesion molecules and cytokines by the endothelial cells.
Under angiogenic-stimulating conditions, the potential to form capillary-like
structures in coculture with dermal fibroblasts was not inhibited. Furthermore,
cytotoxicity studies (the MTS and crystal violet assay) after short- and long-term
exposure to the materials demonstrated that both systems exhibited less toxicity
than solutions of the antiseptic agents alone in comparable concentrations. The
results indicate that responsive antimicrobial nanocomposites could be used as an
advanced drug delivery system and a promising addition to current best practice
wound infection prophylaxis with few side effects.
AN - WOS:000357032100001
AU - Grutzner, V.
AU - Unger, R. E.
AU - Baier, G.
AU - Choritz, L.
AU - Freese, C.
AU - Bose, T.
AU - Landfester, K.
AU - Kirkpatrick, C. J.
DO - 10.2147/IJN.S81263
PY - 2015
SN - 1178-2013
SP - 4111-4124
ST - Enzyme-responsive nanocomposites for wound infection prophylaxis in burn
management: in vitro evaluation of their compatibility with healing processes
TI - Enzyme-responsive nanocomposites for wound infection prophylaxis in burn
management: in vitro evaluation of their compatibility with healing processes
VL - 10
ID - 6642
ER -
TY - JOUR
AB - Nontypeable Haemophilus influenzae (NTHi) is a major pathogen causing otitis
media in children. NTHi releases lipooligosaccharide (LOS) as outer membrane
fragments during its growth. The release of LOS may play an important role in the
pathogenicity of otitis media caused by this organism. The amounts of LOS in
bacterial cells and growth media for five NTHi strains were determined by
quantitative silver staining after sodium dodecyl sulfate-polyacrylamide gel
electrophoresis. These strains were estimated to have 1.6 x 10(6) to 4.8 x 10(6)
LOS molecules per bacterium, During a 3-day growth period, these NTHi strains
released variable but significant amounts of LOS into the growth medium. Cells
started to release detectable amounts of LOS into the medium st 2 to 5 h and
continued to do so for up to 48 or 72 h. The concentrations of LOS in the culture
supernatants released by these five strains were 10 to 55 mu g/ml at 24 h and 40 to
100 mu g/ml at 72 h, which was 34 to 189% of the cell-bound LOS concentration, The
biological properties of released and cell-bound LOSs from two representative
strains were compared. Released LOS showed an approximately 10-fold increase in
inducing human monocytes to produce tumor necrosis factor alpha, interleukin 1
beta, and interleukin 6, a 13- to 28-fold increase in mouse lethal toxicity, and a
16- to 37-fold increase in the clotting of Limulus amebocyte lysate, These results
suggested that released LOS or its inflammatory mediators play a more important
role than the LOS in bacteria in the pathogenicity of otitis media caused by this
organism.
AN - WOS:A1995RW00600052
AU - Gu, X. X.
AU - Tsai, C. M.
AU - Apicella, M. A.
AU - Lim, D. J.
DA - OCT
DO - 10.1128/IAI.63.10.4115-4120.1995
IS - 10
PY - 1995
SN - 0019-9567
SP - 4115-4120
ST - QUANTITATION AND BIOLOGICAL PROPERTIES OF RELEASED AND CELL-BOUND
LIPOOLIGOSACCHARIDES FROM NONTYPABLE HAEMOPHILUS-INFLUENZAE
T2 - INFECTION AND IMMUNITY
TI - QUANTITATION AND BIOLOGICAL PROPERTIES OF RELEASED AND CELL-BOUND
LIPOOLIGOSACCHARIDES FROM NONTYPABLE HAEMOPHILUS-INFLUENZAE
VL - 63
ID - 6659
ER -
TY - JOUR
AB - Nontypeable Haemophilus influenzae (NTHi) is a major pathogen causing otitis
media in children. NTHi releases lipooligosaccharide (LOS) as outer membrane
fragments during its growth. The release of LOS may play an important role in the
pathogenicity of otitis media caused by this organism. The amounts of LOS in
bacterial cells and growth media for five NTHi strains were determined by
quantitative silver staining after sodium dodecyl sulfate- polyacrylamide gel
electrophoresis. These strains were estimated to have 1.6 x 106 to 4.8 x 106 LOS
molecules per bacterium. During a 3-day growth period, these NTHi strains released
variable but significant amounts of LOS into the growth medium. Cells started to
release detectable amounts of LOS into the medium at 2 to 5 h and continued to do
so for up to 48 or 72 h. The concentrations of LOS in the culture supernatants
released by these five strains were 10 to 55 μg/ml at 24 h and 40 to 100 μg/ml at
72 h, which was 34 to 189% of the cell-bound LOS concentration. The biological
properties of released and cell-bound LOSs from two representative strains were
compared. Released LOS showed an approximately 10-fold increase in inducing human
monocytes to produce tumor necrosis factor alpha, interleukin 1β, and interleukin
6, a 13- to 28-told increase in mouse lethal toxicity, and a 16- to 37-told
increase in the clotting of Limulus amebocyte lysate. These results suggested that
released LOS or its inflammatory mediators play a more important role than the LOS
in bacteria in the pathogenicity of otitis media caused by this organism.
AU - Gu, X. X.
AU - Tsai, C. M.
AU - Apicella, M. A.
AU - Lim, D. J.
DB - Scopus
DO - 10.1128/iai.63.10.4115-4120.1995
IS - 10
KW - oligosaccharide
article
bacterial virulence
Haemophilus influenzae
human
human cell
immune response
otitis media
outer membrane
pathogenicity
priority journal
M3 - Article
PY - 1995
SP - 4115-4120
ST - Quantitation and biological properties of released and cell-bound
lipooligosaccharides from nontypeable Haemophilus influenzae
T2 - Infection and Immunity
TI - Quantitation and biological properties of released and cell-bound
lipooligosaccharides from nontypeable Haemophilus influenzae
0029084577&doi=10.1128%2fiai.63.10.4115-
4120.1995&partnerID=40&md5=4148596a1df62cc11ad817b299ae6ccc
VL - 63
ID - 5821
ER -
TY - JOUR
AB - Nanoparticulate materials are produced by industrial processing or engineered
for specific biomedical applications. In both cases, their contact with the human
body may lead to adverse reactions. Most of the published papers so far have
focused on the cytotoxic effects of nanoparticles (NPs). Instead, the present in
vitro study investigates the effect of different types of NP on key components of
the host response such as clot formation and the inflammatory cells. The different
NPs were pre-conditioned with platelet-rich human plasma for 30 min and then
incubated with the blood mononuclear cells for 20 hours. The potential of the
different NPs to induce clot formation, platelet activation and monocyte/macrophage
differentiation was assessed by morphological analysis, immunocytochemistry and
biochemical assays. The data showed that nanoparticulate materials based on
antimony, silver and nickel were capable of promoting the polymerization of fibrin
and the aggregation and fragmentation of platelets, leading to a moderately
activated monocyte phenotype. This process was more pronounced in the case of
antimony- and silver-based NPs that share a similar size and round-shaped
morphology. Conversely, NPs of cobalt, titanium and iron appeared to stimulate
cells to acquire a macrophage phenotype able to secrete higher levels of tumour
necrosis factor α, a pro-inflammatory cytokine. Therefore, the present study
provides clear indications about the subtle and adverse effects that the invasion
of these materials may produce in the cardiovascular system and in vital organs. ©
2009 The Royal Society.
AU - Guildford, A. L.
AU - Poletti, T.
AU - Osbourne, L. H.
AU - Di Cerbo, A.
AU - Gatti, A. M.
AU - Santin, M.
DB - Scopus
DO - 10.1098/rsif.2009.0021
IS - 41
KW - Clot formation
Fibrin
Macrophages
Nanoparticles
Platelets
Activation analysis
Antimony
Blood substitutes
Cobalt
Silver
Titanium
Tumors
antimony
cobalt
fibrin
iron
nanoparticle
nickel
platelet derived growth factor BB
silver
titanium
vasculotropin
Adverse effect
Biochemical assay
Blood mononuclear cells
Cellular components
Cytokines
Cytotoxic effects
Host response
Human bodies
Human plasmas
Immunocytochemistry
In-vitro
Industrial processing
Inflammatory cells
Key component
Morphological analysis
Nano particulates
Necrosis factors
Platelet activation
adult
article
blood clotting
cellular immunity
chemical analysis
controlled study
cytotoxicity
female
human
human cell
immune response
immunocytochemistry
in vitro study
incubation time
inflammatory cell
male
mononuclear cell
polymerization
scanning electrochemical microscopy
thrombocyte rich plasma
M3 - Article
PY - 2009
SP - 1213-1221
ST - Nanoparticles of a different source induce different patterns of activation
in key biochemical and cellular components of the host response
T2 - Journal of the Royal Society Interface
TI - Nanoparticles of a different source induce different patterns of activation
in key biochemical and cellular components of the host response
71049165212&doi=10.1098%2frsif.2009.0021&partnerID=40&md5=76ace3ef3797a633d7e16a4ea
30aa172
VL - 6
ID - 5669
ER -
TY - JOUR
AB - The development of nanotechnologies is leading to greater abundance of
engineered nanoparticles (EN) in the environment, including in the atmospheric air.
To date, it has been shown that the most prevalent EN found in the air are silver
(Ag), titanium dioxide (TiO2), titanium (Ti), and silicon dioxide (SiO2). As the
intestinal tract is increasingly recognized as a target for adverse effects induced
by inhalation of air particles, the aim of this study was to assess the impact of
these 4 atmospheric EN on intestinal inflammation and microbiota. We assessed the
combined toxicity effects of Ag, Ti, TiO2, and SiO2 following a 28-day inhalation
protocol in male and female mice. In distal and proximal colon, and in jejunum, EN
mixture inhalation did not induce overt histological damage, but led to a
significant modulation of inflammatory cytokine transcript abundance, including
downregulation of Tnfα, Ifnγ, Il1β, Il17a, Il22, IL10, and Cxcl1 mRNA levels in
male jejunum. A dysbiosis was observed in cecal microbiota of male and female mice
exposed to the EN mixture, characterized by sex-dependent modulations of specific
bacterial taxa, as well as sex-independent decreased abundance of the
Eggerthellaceae family. Under dextran sodium sulfate-induced inflammatory
conditions, exposure to the EN mixture increased the development of colitis in both
male and female mice. Moreover, the direct dose-response effects of individual and
mixed EN on gut organoids was studied and Ag, TiO2, Ti, SiO2, and EN mixture were
found to generate specific inflammatory responses in the intestinal epithelium.
These results indicate that the 4 most prevalent atmospheric EN could have the
ability to disturb intestinal homeostasis through direct modulation of cytokine
expression in gut epithelium, and by altering the inflammatory response and
microbiota composition following inhalation. © 2022 The Authors
AU - Guilloteau, E.
AU - Djouina, M.
AU - Caboche, S.
AU - Waxin, C.
AU - Deboudt, K.
AU - Beury, D.
AU - Hot, D.
AU - Pichavant, M.
AU - Dubuquoy, L.
AU - Launay, D.
AU - Vignal, C.
AU - Choël, M.
AU - Body-Malapel, M.
C7 - 113442
DB - Scopus
DO - 10.1016/j.ecoenv.2022.113442
KW - Colitis
Digestive tract
Engineered nanoparticles
Inflammation
Inhalation
Intestine
Microbiome
Mixture
Organoid
Animals
Cytokines
Female
Male
Mice
Microbiota
Nanoparticles
Silicon Dioxide
Titanium
cytokine
dextran sulfate
silica nanoparticle
silver nanoparticle
titanium
nanoparticle
silicon dioxide
titanium dioxide
digestive system
nanotechnology
rodent
silicon
silver
animal experiment
animal tissue
Article
ascending colon
atmospheric particulate matter
colitis
controlled study
Cxcl1 gene
descending colon
dose response
down regulation
dysbiosis
Eggerthellaceae
enteritis
environmental impact assessment
female
gene
genetic transcription
homeostasis
Ifngamma gene
IL10 gene
Il17a gene
Il1beta gene
Il22 gene
inhalation
intestine epithelium
intestine flora
jejunum
male
modulation
mouse
nanoengineering
nonhuman
organoid
population abundance
species composition
taxonomy
tissue injury
Tnfa gene
animal
genetics
metabolism
microflora
M3 - Article
PY - 2022
ST - Exposure to atmospheric Ag, TiO2, Ti and SiO2 engineered nanoparticles
modulates gut inflammatory response and microbiota in mice
TI - Exposure to atmospheric Ag, TiO2, Ti and SiO2 engineered nanoparticles
modulates gut inflammatory response and microbiota in mice
85127225863&doi=10.1016%2fj.ecoenv.2022.113442&partnerID=40&md5=b307503dd2cb07a8f17
261a6f46c5930
VL - 236
ID - 5130
ER -
TY - JOUR
AB - Pinus roxburghii Sarg., commonly known as “chir pine,” belonging to the
family Pinaceae, is found in the Himalayan region of Tibet, Bhutan, Nepal,
Pakistan, and North Indian provinces. Due to its medicinal properties, it has been
used by folklore for treating several ailments. Several medicinal characteristics
like wound healing activity, cognitive enhancement, anti-inflammatory, anti-
diabetic, anti-dyslipidemic, anticonvulsant, hepatoprotection, and anticancer
properties have been proved scientifically. The medicinal properties of P.
roxburghii can be attributed to the presence of various secondary metabolites. This
review focuses on medicinal properties and secondary metabolites present in P.
roxburghii found in different regions like Egypt, India, Nepal, and Pakistan as
analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). GC-MS analysis for
secondary metabolites of P. roxburghii species from Egypt, India, and Pakistan
revealed dominated compounds like α-pinene, 3-carene, caryophyllene, whereas P.
roxburghii samples from Nepal showed major compounds like terpinen- 4-ol and
eugenol. Similarly, samples from Egypt were also found to be enriched with α-
thujene and d-longifolene. On the other hand, α-terpinene and α-phellandrene were
identified as major compounds in samples from India. These variations in the
phytocomponents of P. roxburghii can be attributed to the geographical conditions
of these places. © 2021, Indian journals. All rights reserved.
AU - Gulilat, H.
AU - Saini, A. K.
AU - Saini, R. V.
DB - Scopus
DO - 10.5958/0975-6892.2021.00046.0
IS - 3
KW - GC-MS
Geographical location
Natural product
P. roxburghii
Phytoconstituents
3 carene
acetic acid ethyl ester
acetone
albumin
alcohol
aldehyde reductase
alkaloid
alpha phellandrene
alpha thujene
anthracene
baculoviral IAP repeat containing protein 5
bilirubin
butanol
camphene
carbohydrate
cardiac glycoside
caryophyllene
caspase 3
chloroform
cholesterol
coumarin
dipeptidyl peptidase IV
essential oil
eugenol
flavonoid
FLICE inhibitory protein
gelatinase B
glutathione
glycerol
glycoside
hexane
high density lipoprotein cholesterol
insulin receptor
interleukin 6
lignan
limonene
malonaldehyde
methanol
myeloperoxidase
nitric oxide
ocimene
palmitic acid
phenol
phosphorothioic acid o,o,o trimethyl ester
phytochemical
pinene
pinoresinol
plant extract
poly(adenosine diphosphate ribose)
protein bcl 2
protein bcl x
protein bcl xl
protein tyrosine phosphatase 1B
resin
RNA directed DNA polymerase
saponin
sesquiterpene
silver nanoparticle
stilbene
sugar
tannin
terpenoid
terpinen 4 ol
terpinene
triacylglycerol
turpentine oil
water
xanthone derivative
A-549 cell line
Agrobacterium tumefaciens
alkaline phosphatase blood level
antiaging activity
anticonvulsant activity
antimalarial activity
antiviral activity
apoptosis
Article
bilirubin blood level
bioassay
Candida albicans
cholesterol synthesis
cognition
cytotoxicity
distillation
electric shock
Enterobacter aerogenes
epithelization
Escherichia coli
folklore
frontal cortex
HCT 116 cell line
hippocampus
KBM-5 cell line
liver histology
liver injury
liver protection
lymphocytic infiltration
MCF-7 cell line
mental disease
necrosis
neuroprotection
nonhuman
oxidative stress
pentylenetetrazole-induced seizure
pine
SCC-4 cell line
systematic review
T-47D cell line
U266B1 cell line
wound healing
M3 - Article
PY - 2021
SP - 396-408
ST - The comparative analysis of pinus roxburghii phytoconstituents found at
various asian locations as determined by gc-ms and its medicinal properties
T2 - Medicinal Plants
TI - The comparative analysis of pinus roxburghii phytoconstituents found at
various asian locations as determined by gc-ms and its medicinal properties
85118489423&doi=10.5958%2f0975-
6892.2021.00046.0&partnerID=40&md5=08217113edbdc33b87bc3aea715fca6d
VL - 13
ID - 5203
ER -
TY - JOUR
AB - Experimental modeling to identify specific inhalation hazards for
nanomaterials has in the main focused on in vivo approaches. However, these models
suffer from uncertainties surrounding species-specific differences and cellular
targets for biologic response. In terms of pulmonary exposure, approaches which
combine ‘inhalation-like’ nanoparticulate aerosol deposition with relevant human
cell and tissue air–liquid interface cultures are considered an important
complement to in vivo work. In this study, we utilized such a model system to build
on previous results from in vivo exposures, which highlighted the small airway
epithelium as a target for silver nanoparticle (AgNP) deposition. RNA-SEQ was used
to characterize alterations in mRNA and miRNA within the lung. Organotypic-
reconstituted 3D human primary small airway epithelial cell cultures (SmallAir)
were exposed to the same spark-generated AgNP and at the same dose used in vivo, in
an aerosol-exposure air–liquid interface (AE-ALI) system. Adverse effects were
characterized using lactate, LDH release and alterations in mRNA and miRNA. Modest
toxicological effects were paralleled by significant regulation in gene expression,
reflective mainly of specific inflammatory events. Importantly, there was a level
of concordance between gene expression changes observed in vitro and in vivo. We
also observed a significant correlation between AgNP and mass equivalent silver ion
(Ag+) induced transcriptional changes in SmallAir cultures. In addition to key
mechanistic information relevant for our understanding of the potential health
risks associated with AgNP inhalation exposure, this work further highlights the
small airway epithelium as an important target for adverse effects. © 2018, © 2018
British Crown Copyright, the Met Office. Published by Informa UK Limited, trading
as Taylor & Francis Group.
AU - Guo, C.
AU - Buckley, A.
AU - Marczylo, T.
AU - Seiffert, J.
AU - Römer, I.
AU - Warren, J.
AU - Hodgson, A.
AU - Chung, K. F.
AU - Gant, T. W.
AU - Smith, R.
AU - Leonard, M. O.
DB - Scopus
DO - 10.1080/17435390.2018.1465140
IS - 6
KW - genomics
inflammation
mechanistic toxicology
Small airway
Aerosols
Animals
Cells, Cultured
Epithelium
Humans
Inhalation Exposure
Lung
Male
Metal Nanoparticles
Rats
Silver
complementary DNA
messenger RNA
microRNA
microRNA 1298
microRNA 142
microRNA 146b
microRNA 155
microRNA 192
microRNA 200b
microRNA 21
microRNA 224
microRNA 340
microRNA 423
microRNA 872
microRNA 99b
silver nanoparticle
unclassified drug
metal nanoparticle
silver
aerosol
airway epithelium cell
animal experiment
animal model
animal tissue
Article
cell culture
complement activation
controlled study
cytotoxicity
DNA damage
enzyme release
exposure
human
human cell
in vitro study
in vivo study
lung lavage
lung parenchyma
male
nanotoxicology
nonhuman
oxidative stress
priority journal
rat
respiratory epithelium
respiratory tract inflammation
RNA sequence
signal transduction
tissue injury
animal
drug effect
epithelium
lung
metabolism
Sprague Dawley rat
M3 - Article
PY - 2018
SP - 539-553
ST - The small airway epithelium as a target for the adverse pulmonary effects of
silver nanoparticle inhalation
T2 - Nanotoxicology
TI - The small airway epithelium as a target for the adverse pulmonary effects of
85046722109&doi=10.1080%2f17435390.2018.1465140&partnerID=40&md5=2cd2d90976d7e1acf1
eb70f22cefbf00
VL - 12
ID - 5382
ER -
TY - JOUR
AB - Experimental modeling to identify specific inhalation hazards for
nanomaterials has in the main focused on in vivo approaches. However, these models
suffer from uncertainties surrounding species-specific differences and cellular
targets for biologic response. In terms of pulmonary exposure, approaches which
combine 'inhalation-like' nano-particulate aerosol deposition with relevant human
cell and tissue air-liquid interface cultures are considered an important
complement to in vivo work. In this study, we utilized such a model system to build
on previous results from in vivo exposures, which highlighted the small airway
epithelium as a target for silver nanoparticle (AgNP) deposition. RNA-SEQ was used
to characterize alterations in mRNA and miRNA within the lung. Organotypic-
reconstituted 3D human primary small airway epithelial cell cultures (SmallAir)
were exposed to the same spark-generated AgNP and at the same dose used in vivo, in
an aerosol-exposure air-liquid interface (AE-ALI) system. Adverse effects were
characterized using lactate, LDH release and alterations in mRNA and miRNA. Modest
toxicological effects were paralleled by significant regulation in gene expression,
reflective mainly of specific inflammatory events. Importantly, there was a level
of concordance between gene expression changes observed in vitro and in vivo. We
also observed a significant correlation between AgNP and mass equivalent silver ion
(Ag+) induced transcriptional changes in SmallAir cultures. In addition to key
mechanistic information relevant for our understanding of the potential health
risks associated with AgNP inhalation exposure, this work further highlights the
small airway epithelium as an important target for adverse effects.
AN - WOS:000439981600004
AU - Guo, C.
AU - Buckley, A.
AU - Marczylo, T.
AU - Seiffert, J.
AU - Romer, I.
AU - Warren, J.
AU - Hodgson, A.
AU - Chung, K. F.
AU - Gant, T. W.
AU - Smith, R.
AU - Leonard, M. O.
DO - 10.1080/17435390.2018.1465140
IS - 6
PY - 2018
SN - 1743-5390
1743-5404
SP - 539-553
ST - The small airway epithelium as a target for the adverse pulmonary effects of
T2 - NANOTOXICOLOGY
TI - The small airway epithelium as a target for the adverse pulmonary effects of
VL - 12
ID - 6446
ER -
TY - JOUR
AB - With increasing applications of nanomaterials, including silver nanoparticles
(AgNPs), unknown potential risks are present against humans and the environment,
especially to the fetus and neonates, which are more sensitive to the cytotoxicity
of such agents. This study focused on the effects of AgNP exposure on newborn
neurons differentiated from neural stem cells (NSCs) in vitro. We isolated NSCs
from fetal rat hippocampus and incubated them in neural differentiation medium for
3-7 days to form newborn neurons and networks. After exposure to 2 mu g/mL AgNPs,
cell viability was reduced, and early neuronal processes and extensions were
fragmented. Furthermore, AgNP treatment increased cellular superoxide dismutase
activity and decreased the mitochondrial membrane potential, leading to neuronal
death. AgNPs also increased the expression of FOXO3 and decreased nuclear factor-
erythroid 2-related factor-2, as well as stimulated the formation of
autophagosomes. Therefore, even a low concentration of AgNPs can interrupt early
neuronal processes, and facilitate neuron apoptosis by increased cellular oxidative
stress and mitochondrial disruption. Thus, it is necessary to note the daily
exposure of nanomaterials (e.g., AgNPs) to pregnant women and infants, which may
cause neurodevelopmental disorders.
AN - WOS:000414399400001
AU - Guo, X. Y.
AU - Zhang, G. L.
AU - Chen, L. K.
AU - Khan, A. A.
AU - Gu, B.
AU - Li, B. Q.
C6 - OCT 2017
DA - DEC
DO - 10.1089/dna.2017.3795
IS - 12
PY - 2017
SN - 1044-5498
1557-7430
SP - 1062-1070
ST - Newborn Neurons Are Damaged In Vitro by a Low Concentration of Silver
Nanoparticles Through the Inflammatory Oxidative Stress Pathway
T2 - DNA AND CELL BIOLOGY
TI - Newborn Neurons Are Damaged In Vitro by a Low Concentration of Silver
Nanoparticles Through the Inflammatory Oxidative Stress Pathway
VL - 36
ID - 5918
ER -
TY - JOUR
AB - With increasing applications of nanomaterials, including silver nanoparticles
(AgNPs), unknown potential risks are present against humans and the environment,
especially to the fetus and neonates, which are more sensitive to the cytotoxicity
of such agents. This study focused on the effects of AgNP exposure on newborn
neurons differentiated from neural stem cells (NSCs) in vitro. We isolated NSCs
from fetal rat hippocampus and incubated them in neural differentiation medium for
3-7 days to form newborn neurons and networks. After exposure to 2 μg/mL AgNPs,
cell viability was reduced, and early neuronal processes and extensions were
fragmented. Furthermore, AgNP treatment increased cellular superoxide dismutase
activity and decreased the mitochondrial membrane potential, leading to neuronal
death. AgNPs also increased the expression of FOXO3 and decreased nuclear factor-
erythroid 2-related factor-2, as well as stimulated the formation of
autophagosomes. Therefore, even a low concentration of AgNPs can interrupt early
neuronal processes, and facilitate neuron apoptosis by increased cellular oxidative
stress and mitochondrial disruption. Thus, it is necessary to note the daily
exposure of nanomaterials (e.g., AgNPs) to pregnant women and infants, which may
cause neurodevelopmental disorders. © Mary Ann Liebert, Inc. 2017.
AU - Guo, X.
AU - Zhang, G.
AU - Chen, L.
AU - Khan, A. A.
AU - Gu, B.
AU - Li, B.
DB - Scopus
DO - 10.1089/dna.2017.3795
IS - 12
KW - inflammation
neural stem cells
neuron
Animals
Animals, Newborn
Apoptosis
Autophagosomes
Cells, Cultured
Female
Humans
Inflammation
Metal Nanoparticles
Neural Stem Cells
Neurons
Oxidative Stress
Pregnancy
Rats
Silver
silver nanoparticle
transcription factor FKHRL1
metal nanoparticle
silver
animal cell
animal tissue
apoptosis
Article
autophagosome
cell viability
controlled study
fetus
hippocampus
in vitro study
nerve cell differentiation
neural stem cell
nonhuman
oxidative stress
priority journal
protein expression
rat
animal
cell culture
chemically induced
drug effects
female
human
metabolism
nerve cell
newborn
pathology
pregnancy
Sprague Dawley rat
M3 - Article
PY - 2017
SP - 1062-1070
ST - Newborn neurons are damaged in vitro by a low concentration of silver
nanoparticles through the inflammatory oxidative stress pathway
T2 - DNA and Cell Biology
TI - Newborn neurons are damaged in vitro by a low concentration of silver
nanoparticles through the inflammatory oxidative stress pathway
85038364030&doi=10.1089%2fdna.2017.3795&partnerID=40&md5=10e8f390308e44a69be74ed19c
45c4c9
VL - 36
ID - 5458
ER -
TY - JOUR
AB - The thermoplasmonic properties of platinum nanoparticles (PtNPs) render them
desirable for use in diagnosis, detection, therapy, and surgery. However, their
toxicological effects and impact at the molecular level remain obscure.
Nanotoxicology is mainly focused on the interactions of nanostructures with
biological systems, particularly with an emphasis on elucidating the relationship
between the physical and chemical properties such as size and shape. Therefore, we
hypothesized whether these unique anisotropic nanoparticles could induce
cytotoxicity similar to that of spherical nanoparticles and the mechanism involved.
Thus, we synthesized unique and distinct anisotropic PtNPs using lycopene as a
biological template and investigated their biological activities in model human
acute monocytic leukemia (THP-1) macrophages. Exposure to PtNPs for 24 h dose-
dependently decreased cell viability and proliferation. Levels of the cytotoxic
markers lactate dehydrogenase and intracellular protease significantly and dose-
dependently increased with PtNP concentration. Furthermore, cells incubated with
PtNPs dose-dependently produced oxidative stress markers including reactive oxygen
species (ROS), malondialdehyde, nitric oxide, and carbonylated protein. An
imbalance in pro-oxidants and antioxidants was confirmed by significant decreases
in reduced glutathione, thioredoxin, superoxide dismutase, and catalase levels
against oxidative stress. The cell death mechanism was confirmed by mitochondrial
dysfunction and decreased ATP levels, mitochondrial copy numbers, and PGC-1 alpha
expression. To further substantiate the mechanism of cell death mediated by
endoplasmic reticulum stress (ERS), we determined the expression of the inositol-
requiring enzyme (IRE1), (PKR-like ER kinase) PERK, activating transcription factor
6 (ATF6), and activating transcription factor 4 ATF4, the apoptotic markers p53,
Bax, and caspase 3, and the anti-apoptotic marker Bcl-2. PtNPs could activate ERS
and apoptosis mediated by mitochondria. A proinflammatory response to PtNPs was
confirmed by significant upregulation of interleukin-1-beta (IL-1 beta), interferon
gamma (IFN gamma), tumor necrosis factor alpha (TNF alpha), and interleukin (IL-6).
Transcriptomic and molecular pathway analyses of THP-1 cells incubated with the
half maximal inhibitory concentration (IC50) of PtNPs revealed the altered
expression of genes involved in protein misfolding, mitochondrial function, protein
synthesis, inflammatory responses, and transcription regulation. We applied
transcriptomic analyses to investigate anisotropic PtNP-induced toxicity for
further mechanistic studies. Isotropic nanoparticles are specifically used to
inhibit non-specific cellular uptake, leading to enhanced in vivo bio-distribution
and increased targeting capabilities due to the higher radius of curvature. These
characteristics of anisotropic nanoparticles could enable the technology as an
attractive platform for nanomedicine in biomedical applications.
AN - WOS:000515380000070
AU - Gurunathan, S.
AU - Jeyaraj, M.
AU - La, H.
AU - Yoo, H.
AU - Choi, Y.
AU - Do, J. T.
AU - Park, C.
AU - Kim, J. H.
AU - Hong, K.
C7 - 440
DA - JAN 2
DO - 10.3390/ijms21020440
IS - 2
PY - 2020
SN - 1422-0067
ST - Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis,
Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute
Monocytic Leukemia Cells
TI - Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis,
Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute
Monocytic Leukemia Cells
VL - 21
ID - 6155
ER -
TY - JOUR
AB - The rapid development of nanotechnology has led to the use of silver
nanoparticles (AgNPs) in biomedical applications, including antibacterial,
antiviral, anti-inflammatory, and anticancer therapies. The molecular mechanism of
AgNPs-induced cytotoxicity has not been studied thoroughly using a combination of
cellular assays and RNA sequencing (RNA-Seq) analysis. In this study, we prepared
AgNPs using myricetin, an anti-oxidant polyphenol, and studied their effects on
NIH3T3 mouse embryonic fibroblasts as an in vitro model system to explore the
potential biomedical applications of AgNPs. AgNPs induced loss of cell viability
and cell proliferation in a dose-dependent manner, as evident by increased leakage
of lactate dehydrogenase (LDH) from cells. Reactive oxygen species (ROS) were a
potential source of cytotoxicity. AgNPs also incrementally increased oxidative
stress and the level of malondialdehyde, depleted glutathione and superoxide
dismutase, reduced mitochondrial membrane potential and adenosine triphosphate
(ATP), and caused DNA damage by increasing the level of 8-hydroxy-2′-deoxyguanosine
and the expressions of the p53 and p21 genes in NIH3T3 cells. Thus, activation of
oxidative stress may be crucial for NIH3T3 cytotoxicity. Interestingly, gene
ontology (GO) term analysis revealed alterations in epigenetics-related biological
processes including nucleosome assembly and DNA methylation due to AgNPs exposure.
This study is the first demonstration that AgNPs can alter bulk histone gene
expression. Therefore, our genome-scale study suggests that the apoptosis observed
in NIH3T3 cells treated with AgNPs is mediated by the repression of genes required
for cell survival and the aberrant enhancement of nucleosome assembly components to
induce apoptosis. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Gurunathan, S.
AU - Qasim, M.
AU - Park, C.
AU - Yoo, H.
AU - Choi, D. Y.
AU - Song, H.
AU - Kim, J. H.
AU - Hong, K.
C7 - 3618
DB - Scopus
DO - 10.3390/ijms19113618
IS - 11
KW - Antioxidants
Apoptosis
Cytotoxicity
DNA damage
Epigenetics
Oxidative stress
Animals
Autophagosomes
Cell Proliferation
Cell Survival
DNA Damage
Embryo, Mammalian
Endocytosis
Fibroblasts
Flavonoids
Gene Expression Profiling
Lysosomes
Malondialdehyde
Metal Nanoparticles
Mice
NIH 3T3 Cells
Nucleosomes
Oxidative Stress
Silver
Static Electricity
antioxidant
caspase 3
catalase
cholecystokinin octapeptide
cyclin dependent kinase 2
cyclin dependent kinase 4
deoxyguanosine derivative
glutathione
growth arrest and DNA damage inducible protein 45
growth arrest and DNA damage inducible protein 45 a
malonaldehyde
messenger RNA
myricetin
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
protein p21
protein p53
proteinase
silver nanoparticle
unclassified drug
flavonoid
metal nanoparticle
silver
animal cell
apoptosis
Article
bioinformatics
BrdU assay
controlled study
cytotoxicity
DNA fragmentation
drug analysis
drug cytotoxicity
drug formulation
drug mechanism
drug uptake
fibroblast culture
gene expression
genotoxicity
internalization
lipid peroxidation
mouse
NIH 3T3 cell line
nonhuman
oxidative stress
particle size
physical chemistry
RNA sequence
transcriptomics
upregulation
zeta potential
animal
autophagosome
cell proliferation
cell survival
cytology
drug effect
endocytosis
fibroblast
genetics
lysosome
mammalian embryo
metabolism
nucleosome
static electricity
ultrastructure
M3 - Article
PY - 2018
ST - Cytotoxicity and transcriptomic analysis of silver nanoparticles in mouse
embryonic fibroblast cells
TI - Cytotoxicity and transcriptomic analysis of silver nanoparticles in mouse
embryonic fibroblast cells
85056691798&doi=10.3390%2fijms19113618&partnerID=40&md5=383899a4e891d0731d9676b9250
2e1ed
VL - 19
ID - 5387
ER -
TY - JOUR
AB - Background Gold nanoparticles (AuNPs) have potential for a wide range of
applications as therapeutic and diagnostic agents. Since they have a high
probability of interacting with human immune cells, cytotoxicity studies must be
conducted. The investigation of AuNP/immune cell interaction has mainly focused on
macrophages and dendritic cells, along with some other cell lineages. Scarce
information is available regarding the effect of AuNPs on mast cells, which are
abundant in the skin, mucosa, and perivascular space. Objective To examine the
uptake of AuNPs by HMC-1 human mast cells and the resulting effect on cell
viability, pro-inflammatory mediators production, and proliferation. Results With
AuNPs treatment, the viability of HMC-1 cells decreased slightly (never less than
95%) during the first 4 h, but no changes were detected in the proliferation rate
at any time. Increasing concentrations of AuNPs produced greater cell granularity
(uptake). CLSM images exhibited AuNPs clusters in the cell cytoplasm. TNF-alpha and
ROS production was not stimulated by AuNPs treatment at any concentration/time.
Conclusion Internalization of AuNPs into HMC-1 cells was demonstrated in an in
vitro model, without showing cytotoxic effects or induction of pro-inflammatory
mediators at any concentration tested.
AN - WOS:000673490400001
AU - Gutierrez-Calleja, R. A.
AU - Rodriguez-Cortes, O.
AU - Flores-Mejia, R.
AU - Munoz-Diosdado, A.
C6 - JUL 2021
DA - OCT
DO - 10.1007/s13273-021-00152-7
IS - 4
PY - 2021
SN - 1738-642X
2092-8467
SP - 439-452
ST - Gold nanoparticles: uptake in human mast cells and effect on cell viability,
inflammatory mediators, and proliferation
T2 - MOLECULAR & CELLULAR TOXICOLOGY
TI - Gold nanoparticles: uptake in human mast cells and effect on cell viability,
inflammatory mediators, and proliferation
VL - 17
ID - 6272
ER -
TY - JOUR
AB - In recent years, there have been remarkable efforts to examine and understand
the adverse effects of nanoparticles (NPs) on the environment and human health, not
only qualitatively but also quantitatively. Mass cytometry has been developed for
high-dimensional single-cell analyses and used to quantify the cellular association
of inorganic NPs. Here, we have applied this novel technique to investigate the
heterogeneity in cellular association and cytotoxicity of polyvinylpyrrolidone-
coated silver nanoparticles with diameters of 10 nm and 20 nm in primary human
immune cells. Our results revealed the cell-type-dependent heterogeneity in which
AgNPs showed higher affinity to phagocytic cells like monocytes and dendritic cells
than to other immune cell types. Upon exposure to AgNPs, these cells exhibited
complex pro-inflammatory and pro-apoptotic responses, such as IκBα degradation,
STAT1 phosphorylation and caspase-7 activation. Quantitative analyses of the
single-cell dose-response relationship between the cellular AgNP association and
signalling activities further revealed heterogeneity even within a monocyte
population. The majority of cells belonged to the 'low affinity' subset, which
showed a positive AgNP dose-cisplatin uptake (i.e. viability loss) correlation, as
opposed to the remaining cells which belonged to the 'high affinity' subset and had
an insignificant relationship between the cell-associated AgNP amount and cisplatin
uptake/viability loss level. These subsets were distinctly responsive to the
cellular AgNP content, as they showed different levels of signalling proteins such
as IκBα, STAT1 and caspase-7. Our study can be helpful for further understanding
the heterogeneous nature of cell-NP interactions and development of dose-response
models at the single-cell level for various NPs, which will provide key information
for the safe use of nanomaterials in biomedical applications. This journal is © The
Royal Society of Chemistry.
AU - Ha, M. K.
AU - Choi, J. S.
AU - Kwon, S. J.
AU - Song, J.
AU - Lee, Y.
AU - Kim, Y. E.
AU - Yoon, T. H.
DB - Scopus
DO - 10.1039/c9en01104h
IS - 4
KW - Cells
Cytology
Metal nanoparticles
Apoptotic response
Cellular association
Dose response relationships
Dose-response models
Poly vinyl pyrrolidone
Signalling proteins
Singlecell analysis
apoptosis
cell
nanoparticle
silver
Cell signaling
M3 - Article
PY - 2020
SP - 1102-1114
ST - Mass cytometric study on the heterogeneity in cellular association and
cytotoxicity of silver nanoparticles in primary human immune cells
T2 - Environmental Science: Nano
TI - Mass cytometric study on the heterogeneity in cellular association and
cytotoxicity of silver nanoparticles in primary human immune cells
85081742245&doi=10.1039%2fc9en01104h&partnerID=40&md5=10366e3c8dc40f34df191f461030a
396
VL - 7
ID - 5273
ER -
TY - JOUR
AB - Silver is commonly used as an antibacterial agent, e. g., in various medical
applications, and the availability of silver nanoparticles (AgNP) has fueled this
development. Their antibacterial properties are well defined, whereas there are
concerns regarding unknown and potentially harmful effects of AgNPs on immune cells
and an ongoing immune reaction. Aim of the present study is a comparison of the
effects of AgNPs and ionic silver (Ag+) on cells of the innate immune system, in
particular on neutrophil granulocytes and macrophages. The AgNPs were synthesized
within hydroxylated polyester dendrimer templates via an in situ approach,
generating five kinds of AgNPs with mean diameters from 2.0 to 34.7 nm. 4 No impact
is observed on phagocytosis and oxidative burst, as well as activation of the
promoter for the pro-inflammatory cytokine TNF-alpha. In contrast, both AgNPs and
Ag+, but not the dendrimer templates, trigger the release of neutrophil
extracellular traps and inhibit the formation of nitric monoxide. On the molecular
level, AgNPs and Ag+ cause elevated intracellular levels of reactive oxygen species
and the second messenger Zn2+. Moreover, protein phosphatases are inhibited by an
oxidative mechanism. Taken together, there are several effects of AgNPs on
neutrophil granulocytes and macrophages in vitro, but these are not specific for
AgNP, instead they are also observed with Ag+, and Ag+ released from AgNPs seems to
be the component responsible for most of the particles' immunomodulatory activity.
AN - WOS:000328729400024
AU - Haase, H.
AU - Fahmi, A.
AU - Mahltig, B.
DA - JUN
DO - 10.1166/jbn.2014.1784
IS - 6
PY - 2014
SN - 1550-7033
1550-7041
SP - 1146-1156
ST - Impact of Silver Nanoparticles and Silver Ions on Innate Immune Cells
T2 - JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
TI - Impact of Silver Nanoparticles and Silver Ions on Innate Immune Cells
VL - 10
ID - 6069
ER -
TY - JOUR
AB - The synthesis and characterization of the silver(I) chloride complex of
formula { [ AgCI(CMBZT) (TPTP)2 ]̇(MeOH) } (1) (CMBZT = 5-chloro-2-
mercaptobenzothiazole, TPTP = tris(p-tolyl)phosphine) is described. Also the
structure of the hydrate derivative { [ AgCI (TPTP)3 ]̇(0.5̇ H2 O) } (2) of the
corresponding known anhydrous silver complex (Zartilas et al., 2009), and the
polymorph 3 of the known [ AgI (TPTP)3 ] complex (Zartilas et al., 2009) were
determined and compared with the known ones. In addition, the structure of the
known one silver(I) cluster { [ AgI(TPTP) ] 4 } (4) (Meijboom et al., 2009) was re-
determined at 120(2) K and possible Ag-Ag interactions were analyzed. The compounds
1-4 were characterized by X-ray crystallography at r.t (1) and 120 K (2-4). All
these complexes and { [ (Et3 NH) + ] 2 [ Ag6 ( μ3 -Hmna)4 ( μ3-mna)2 ]2- (DMSO)2 (H
2 O) } (5) (Hmna = 2-mercaptonicotinic acid) were evaluated for cytotoxic and anti-
inflammatory activity. The in vitro testing of cytotoxic activity of 1-5 against
leiomyosarcoma cancer cells (LMS), were evaluated with Trypan Blue and Thiazolyl
Blue Tetrazolium Bromide or 3-(4.5-dimethylthiazol-2- yl)-2.5-diphenyltetrazolium
bromide (MTT) assays. The flow cytometry assay for complex 1 and showed that at
15μM of 1, 62.38 of LMS cells undergo apoptosis, while 7 of LMS cells undergo cell
necrosis. The antitumor activity of 3 is comparable with that of its reported
polymorph (Zartilas et al., 2009). The anti-inflammatory, activity of complexes 1-3
and 5 was also studied. The activity towards cell viability was 2>3>5>1>4, while
the order of the inhibitory activity in cell growth proliferation follows the
order, 2>3>1>4>5. The anti-inflammatory activity on the other hand is 1>2>5>⋯>3. ©
2010 L. Kyros et al.
AU - Kyros, L.
AU - Kubicki, M.
AU - Male, L.
AU - Hursthouse, M. B.
AU - Verginadis, I. I.
AU - Gouma, E.
AU - Karkabounas, S.
C7 - 386860
DB - Scopus
DO - 10.1155/2010/386860
M3 - Article
PY - 2010
ST - Structural properties, cytotoxicity, and anti-inflammatory activity of
silver(I) Complexes with tris(p-tolyl)Phosphine and 5-Chloro-2-
mercaptobenzothiazole
T2 - Bioinorganic Chemistry and Applications
TI - Structural properties, cytotoxicity, and anti-inflammatory activity of
silver(I) Complexes with tris(p-tolyl)Phosphine and 5-Chloro-2-
mercaptobenzothiazole
77951494263&doi=10.1155%2f2010%2f386860&partnerID=40&md5=6de2822ac6089266529b12c9ad
e819ea
VL - 2010
ID - 5700
ER -
TY - JOUR
AB - The increasing emergence of antibiotic resistance coupled with the limited
effectiveness of current treatments highlights the need for the development of new
treatment modalities. Silver nanoparticles (AgNPs) are a promising alternative with
broad-spectrum antibacterial activity. However, the clinical translation of AgNPs
have been hampered primarily due to the delivery of unsafe levels of silver ions
(Ag+) resulting in cellular toxicity and their susceptibility to aggregation
resulting in loss of efficacy. Here, we describe a safe and effective, thermo-
responsive AgNP hydrogel that provides antibacterial effects in conjunction with
wound promoting properties. Using a murine model of wound infection, we demonstrate
that the applied AgNP hydrogel to the wound (12 µg silver) not only provides
superior bactericidal activity but also reduces inflammation leading to accelerated
wound closure when compared to industry-standard silver sulfadiazine (302 µg
silver). The AgNP hydrogel-treatment significantly accelerated wound closure at day
4 post-infection (56 closure) compared to both blank hydrogel or Ag SD (74% and 91%
closure respectively) with a concurrent increase in PCNA-positive proliferating
cells corresponding with a significant 32% improvement in wound re-epithelization
compared to the blank hydrogel. Treatment of infected wounds with AgNP hydrogel
also decreased neutrophil infiltration, increased anti-inflammatory Ym-1 positive
M2 macrophages, and reduced the number of caspase-1 positive apoptotic cells.
Therefore, this novel multifunctional AgNP thermo-responsive hydrogel is
potentially a safe and effective treatment at much lower concentration for the
treatment of wound infections. Statement of significance: In this study, we
describe the development of a multifunctional thermo-responsive hydrogel of
ultrasmall silver nanoparticles (AgNPs) for controlled and optimized delivery of
silver to infected wounds. The in vivo biological effects of the developed hydrogel
showed significant S. aureus elimination from infected mouse wounds compared to a
commercial antibacterial formulation. The developed AgNP hydrogel optimally
regulates inflammatory responses to promote wound healing as indicated by increased
cell proliferation and wound re-epithelization. Additionally, AgNP hydrogel shows
significant potential in regulating neutrophil infiltration while increasing levels
of anti-inflammatory M2 macrophages and reduces the number of apoptotic cells.
Therefore, the multifunctional properties of the developed AgNP thermo-responsive
hydrogel offers great clinical potential to control bacterial infections and
promote wound healing. © 2021 Acta Materialia Inc.
AU - Haidari, H.
AU - Bright, R.
AU - Strudwick, X. L.
AU - Garg, S.
AU - Vasilev, K.
AU - Cowin, A. J.
AU - Kopecki, Z.
DB - Scopus
DO - 10.1016/j.actbio.2021.04.007
KW - Antibacterial
Chronic wound
Controlled release
In vivo wound infection
Thermo-responsive
Topical silver application
Wound healing
Animals
Hydrogels
Metal Nanoparticles
Methicillin-Resistant Staphylococcus aureus
Mice
Silver
Wound Healing
Wound Infection
Cell proliferation
Macrophages
Metal ions
Metal nanoparticles
cycline
hydrogel
interleukin 10
interleukin 1beta
multifunctional nanoparticle
poloxamer
silver nanoparticle
sulfadiazine silver
antiinfective agent
metal nanoparticle
silver
Antibacterials
Chronic wounds
In-vivo
Infected wounds
Wound infections
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
bacterial viability
cell proliferation
controlled study
drug efficacy
drug formulation
drug safety
epithelization
in vivo study
mouse
nonhuman
wound closure
wound healing
wound infection
animal
pharmacology
M3 - Article
PY - 2021
SP - 420-434
ST - Multifunctional ultrasmall AgNP hydrogel accelerates healing of S. aureus
infected wounds
TI - Multifunctional ultrasmall AgNP hydrogel accelerates healing of S. aureus
infected wounds
85106251829&doi=10.1016%2fj.actbio.2021.04.007&partnerID=40&md5=d4d4dd3f8b3d1fbab9e
6ea9b263d8e5c
VL - 128
ID - 5212
ER -
TY - JOUR
AB - Shigella and Salmonella kill host cells and trigger inflammatory responses by
mechanisms that are not fully understood. The goal of this review is to reevaluate
key observations reported over the past 15 years and, whenever possible, to provide
a chronological perspective as to how our understanding of the pathways by which
Shigella and Salmonella kill host cells has evolved. Published by Elsevier SAS.
AN - WOS:000235790200032
AU - Haimovich, B.
AU - Venkatesan, M. M.
DA - FEB
DO - 10.1016/j.micinf.2005.08.002
IS - 2
PY - 2006
SN - 1286-4579
SP - 568-577
ST - Shigella and Salmonella: death as a means of survival
T2 - MICROBES AND INFECTION
TI - Shigella and Salmonella: death as a means of survival
VL - 8
ID - 6687
ER -
TY - JOUR
AB - Biomaterials applications have rapidly expanded into different fields of
sciences. One of the important fields of using biomaterials is dentistry, which can
facilitate implantation, surgery, and treatment of oral diseases such as peri-
implantitis, periodontitis, and other dental problems. Drug delivery systems based
on biocompatible materials play a vital role in the release of drugs into aim
tissues of the oral cavity with minimum side effects. Therefore, scientists have
studied various delivery systems to improve the efficacy and acceptability of
therapeutic approaches in dental problems and oral diseases. Also, biomaterials
could be utilized as carriers in biocompatible drug delivery systems. For instance,
natural polymeric substances, such as gelatin, chitosan, calcium phosphate,
alginate, and xanthan gum are used to prepare different forms of delivery systems.
In addition, some alloys are conducted in drug complexes for the better in
transportation. Delivery systems based on biomaterials are provided with different
strategies, although individual biomaterial has advantages and disadvantages which
have a significant influence on transportation of complex such as solubility in
physiological environments or distribution in tissues. Biomaterials have
antibacterial and anti-inflammatory effects and prolonged time contact and even
enhance antibiotic activities in oral infections. Moreover, these biomaterials are
commonly prepared in some forms such as particulate complex, fibers, microspheres,
gels, hydrogels, and injectable systems. In this review, we examined the
application of biocompatible materials in drug delivery systems of oral and dental
diseases or problems. © 2021 Lotfollah Kamali Hakim et al.
AU - Hakim, L. K.
AU - Yazdanian, M.
AU - Alam, M.
AU - Abbasi, K.
AU - Tebyaniyan, H.
AU - Tahmasebi, E.
AU - Khayatan, D.
AU - Seifalian, A.
AU - Ranjbar, R.
AU - Yazdanian, A.
C7 - 9011226
DB - Scopus
DO - 10.1155/2021/9011226
KW - alginic acid
antibiotic agent
antiinfective agent
biomaterial
calcium phosphate
chitosan
drug carrier
gel
gelatin
gold nanoparticle
hyaluronic acid
hydrogel
metal nanoparticle
microsphere
silver nanoparticle
stabilizing agent
biocompatibility
bone development
complex formation
human
injection
mouth cavity
mouth disease
mouth infection
nonhuman
Review
tooth disease
M3 - Review
PY - 2021
ST - Biocompatible and Biomaterials Application in Drug Delivery System in Oral
Cavity
T2 - Evidence-based Complementary and Alternative Medicine
TI - Biocompatible and Biomaterials Application in Drug Delivery System in Oral
Cavity
85119981141&doi=10.1155%2f2021%2f9011226&partnerID=40&md5=19d3dc6c88b6809ee34422a2f
bd3ab59
VL - 2021
ID - 5181
ER -
TY - JOUR
AB - Periodontal disease is a set of inflammatory conditions affecting the tissues
surrounding the teeth, which can be fairly preventable. In this research,
antibacterial polymeric nanofibers were prepared for the management of periodontal
disease treatment. New hybrid organic/inorganic nanofibers of chitosan/polyethylene
oxide (CS/PEO) contains doxycycline hyclate (DOXH) (as an antibacterial drug) and
silver/hydroxyapatite/silica (Ag/HA/Si) nanocomposite (CS/PEO-DOXH-Ag/HA/Si)
fabricated by electrospinning technique. The nanofibers were characterized by
Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD),
field emission scanning electron microscope (FE-SEM), thermogravimetric analysis
(TGA) and transmission electron microscopy (TEM). In vitro release profile,
degradation (DE) test, swelling (SW) test, antimicrobial activity and cell
viability were also obtained. The DOXH release profile was measured by ultraviolet
visible (UV–Vis) spectrophotometer in artificial saliva (pH 6.8) at 37 °C. The
application of Ag/HA/Si increased the morphological integrity during the
degradation process and decreased the rate of drug delivery. The electrospun
nanofibers showed an enhanced antibacterial activity against Escherichia coli (E.
coli), Staphylococcus aureus (S. aureus), and Streptococcus mutans (S. mutans)
bacterium strains with the lowest DOXH amount, which can reduce the high dosage of
antibiotics and side effects. Cell viability assays revealed that the prepared
nanofibers had no cytotoxicity against the human gingival fibroblast 2 (HGF-2) cell
line. The results suggest that the prepared organic/inorganic hybrid nanofibers
with drug delivery properties could be a promising candidate for the treatment of
periodontal diseases. © 2022, The Author(s), under exclusive licence to Springer-
Verlag GmbH Germany, part of Springer Nature.
AU - Hakimi, F.
AU - Hashemikia, S.
AU - Sadighian, S.
AU - Ramazani, A.
DB - Scopus
DO - 10.1007/s00289-022-04466-x
IS - 8
KW - Chitosan
Hybrid nanofiber
Local drug delivery
Periodontal disease
Cell culture
Diseases
Drug dosage
Escherichia coli
Nanofibers
Silver
Thermogravimetric analysis
Antibacterial drugs
Antibacterials
Doxycycline
Inflammatory conditions
Nano-fibrous
Release profiles
Silica composites
M3 - Article
PY - 2023
SP - 8703-8723
ST - Nanofibrous chitosan/polyethylene oxide silver/hydroxyapatite/silica
composite as a potential biomaterial for local treatment of periodontal disease
T2 - Polymer Bulletin
TI - Nanofibrous chitosan/polyethylene oxide silver/hydroxyapatite/silica
composite as a potential biomaterial for local treatment of periodontal disease
85138813253&doi=10.1007%2fs00289-022-04466-
x&partnerID=40&md5=46120c5fd3fd271d43ff5140b0d5c62b
VL - 80
ID - 5054
ER -
TY - JOUR
AB - Background: Anthrax is caused by Bacillus anthracis that produce two
exotoxins, lethal toxin and edema toxin. The lethal toxin is composed of the lethal
factor (LF) complexed with the cell binding protective antigen (PA(83), 83 kDa).
Likewise, the edema factor (EF) binds to the PA(83) to form the edema toxin. Once
PA(83) is bound to the host cell surface, a furin-like protease cleaves the full-
length, inactive protein into 63 kDa and 20 kDa antigens (PA(63) and PA(20)).
PA(63) forms a heptamer and is internalized via receptor mediated endocytosis
forming a protease-stable pore, which allows EF and LF to enter the cell and exert
their toxic effects. Both proteolytically cleaved protective antigens (PA(63) and
PA(20) fragments) are found in the blood of infected animals. The 63 kDa protective
antigen PA(63) fragment has been thoroughly studied while little is known about the
PA(20). Methods: In this study we examined the role of PA(20) using high throughput
gene expression analysis of human peripheral blood mononuclear cells (PBMC) exposed
to the PA(20). We constructed a PA mutant in which a Factor X-a proteolytic
recognition site was genetically engineered into the protective antigen PA(83) to
obtain PA(20) using limited digestion of this recombinant PA(83) with trypsin.
Results: Global gene expression response studies indicated modulation of various
immune functions and showed gene patterns indicative of apoptosis via the F-as
pathway in a subset of the lymphoid cells. This finding was extended to include
observations of increased Caspase-3 enzymatic activity and the identification of
increases in the population of apoptotic, but not necrotic cells, based on
differential staining methods. We identified a list of similar to 40 inflammatory
mediators and heat-shock proteins that were altered similarly upon exposure of PBMC
to either rPA(20) or B. anthracis spores/vegetative cells. Conclusion: This study
shows that the PA(20) has an effect on human peripheral blood leukocytes and can
induce apoptosis in the absence of other PA components.
AN - WOS:000260224200002
AU - Hammamieh, R.
AU - Ribot, W. J.
AU - Abshire, T. G.
AU - Jett, M.
AU - Ezzell, J.
C7 - 124
DA - SEP 22
DO - 10.1186/1471-2334-8-124
PY - 2008
SN - 1471-2334
ST - Activity of the Bacillus anthracis 20 kDa protective antigen component
T2 - BMC INFECTIOUS DISEASES
TI - Activity of the Bacillus anthracis 20 kDa protective antigen component
VL - 8
ID - 6776
ER -
TY - JOUR
AB - The present study was conducted to evaluate the antitumor effect of silver
nanoparticles and Alpha-lipoic acid and their combination against 1,2-
dimethylhydrazine (DMH-) induced colorectal carcinogenesis in experimental rats.
Fifty Male wistar albino rats were divided into five groups. Group (1): negative
control group, group (2) colorectal cancer induced rats (CRC) (positive control),
group (3): CRC group treated with silver nanoparticles (CRC+AgNPs), group (4): CRC
induced rats treated with Alpha-lipoic acid (CRC+ALA) and group (5): CRC induced
rats treated with combinations (CRC+AgNPs + ALA). The obtained biochemical results
revealed significant reduction in serum transforming growth factor-beta (TGF-beta),
Tumor necrosis factor alpha(TNF-alpha), C - Reactive Protein (CRP),
carcinoembryonic antigen (CEA) and colon cancer specific antigen-4 (CCSA-4) levels
in CRC induced group treated either with silver nanoparticles or alpha-lipoic acid
and their combinations compared to CRC induced group. Regarding gene expression, we
found significant down regulation in K-RAS gene expression with treatment with
either AgNps or ALA or their combinations comparing to untreated group.
Histopathological study greatly supports these results. In Conclusion; the present
study highlighted the antitumor effect of AgNPs, ALA and their Combination in
ameliorating CRC induced experimentally through its potential anti-inflammatory
effect. Copyright (C) 2013 - All Rights Reserved - Pharmacophore
AN - WOS:000432735400006
AU - Hamza, A.
AU - AlSolami, F.
DA - MAR
IS - 2
PY - 2018
SN - 2229-5402
SP - 45-51
ST - ANTITUMOR ACTIVITY OF SILVER NANOPARTICLES AND ALPHA-LIPOIC ACID COMBINATIONS
IN COLORECTAL CANCER INDUCED EXPERIMENTALLY
T2 - PHARMACOPHORE
TI - ANTITUMOR ACTIVITY OF SILVER NANOPARTICLES AND ALPHA-LIPOIC ACID COMBINATIONS
IN COLORECTAL CANCER INDUCED EXPERIMENTALLY
VL - 9
ID - 6050
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely used as an antibiotic agent in
textiles, wound dressings, medical devices, and appliances such as refrigerators
and washing machines. The increasing use of AgNPs has raised concerns about their
potential risks to human health. Therefore, this study was aimed to determine the
impact of AgNPs in germ cell specific complications in mice. The administration of
AgNPs results in toxicity in mice; however, a more detailed understanding of the
effects of AgNPs on germ cells remains poorly understood. Here, we demonstrate the
effects of AgNPs (20 nm in diameter) in a mouse Sertoli and granulosa cells in
vitro, and in male and female mice in vivo. Soluble silver ion (Ag+)-treated cells
were used as a positive control. We found that excessive AgNP-treated cells
exhibited cytotoxicity, the formation of autophagosomes and autolysosomes in
Sertoli cells. Furthermore, an increase in mitochondrial-mediated apoptosis by
cytochrome c release from mitochondria due to translocation of Bax to mitochondria
was observed. In in vivo studies, the expression of pro-inflammatory cytokines,
including tumor necrosis factor α, interferon-γ, -6, -1β, and monocyte
chemoattractant protein-1 were significantly increased (p < 0.05).
Histopathological analysis of AgNP-treated mice shows that a significant loss of
male and female germ cells. Taken together, these data suggest that AgNPs with an
average size of 20 nm have negative impact on the reproduction. © 2015 Taylor &
Francis.
AU - Han, J. W.
AU - Jeong, J. K.
AU - Gurunathan, S.
AU - Choi, Y. J.
AU - Das, J.
AU - Kwon, D. N.
AU - Cho, S. G.
AU - Park, C.
AU - Seo, H. G.
AU - Park, J. K.
AU - Kim, J. H.
DB - Scopus
DO - 10.3109/17435390.2015.1073396
IS - 3
KW - Apoptosis
germ cells
pro-inflammatory cytokines
Animals
Cell Survival
Cells, Cultured
Female
Fertility
Germ Cells
Lysosomes
Male
Metal Nanoparticles
Mice
Ovary
Phagosomes
Silver
Spermatogenesis
Testis
aromatase
cytochrome c
gamma interferon
hemoglobin
interleukin 1beta
interleukin 6
messenger RNA
protein Bax
silver nanoparticle
autacoid
metal nanoparticle
silver
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
autolysosome
autophagosome
cell viability
controlled study
cytosol
cytotoxicity
female
fetus
flow cytometry
gene expression
gene translocation
granulosa cell
hematocrit
histopathology
male
mean corpuscular hemoglobin
mean corpuscular volume
mitochondrion
mouse
nanotoxicology
nonhuman
oxidative stress
priority journal
protein expression
Sertoli cell
somatic cell
spermatocyte
spermatogenesis
animal
cell culture
cell survival
chemistry
cytology
dose response
drug effects
fertility
germ cell
lysosome
metabolism
ovary
phagosome
testis
TUNEL assay
M3 - Article
PY - 2016
SP - 361-373
ST - Male- and female-derived somatic and germ cell-specific toxicity of silver
nanoparticles in mouse
T2 - Nanotoxicology
TI - Male- and female-derived somatic and germ cell-specific toxicity of silver
nanoparticles in mouse
84959458966&doi=10.3109%2f17435390.2015.1073396&partnerID=40&md5=613a7a3ed3fefc1a7b
cdf038f46f7c7c
VL - 10
ID - 5486
ER -
TY - JOUR
AB - Biocomposite nanomaterials have been evolved as the new generation catalysts
and therapeutic supplement in these days. Magnetically isolation has added new
features to this category. This has encouraged us to synthesize a novel Ag NP
adorned chitosan-alginate dual bio-polysaccharide (two of the more versatile
polysaccharides) modified core-shell magnetic nanocomposite (Fe3O4/CS-Alg/Ag NPs).
The material was meticulously characterized following different physicochemical
techniques, such as, FT-IR, ICP-OES, FESEM, EDX, atomic mapping, TEM, VSM, XRD and
XPS studies. The as synthesized material was catalytically explored in the one-pot
multicomponent synthesis of biologically potent 2H-indazolo[2,1-b]phthalazine-
trione derivatives involving a wide range of substrates. The reactions were ended
up with excellent yields under solvent-free heating conditions. The catalyst
recyclability, heterogeneity and leaching tests were performed to ensure its high
stability and robustness. It could be reused as much as 10 times in succession with
almost unchanged catalytic performances. In the lung protective part of the present
research, the human lung toxicity was induced by α-Guttiferin. The cell viability
of lung MRC-5, CCD[sbnd]19Lu, WI-38, and BEAS-2B cell lines was measured by trypan
blue assay. Caspase-3 activity was assessed by the caspase activity colorimetric
assay kit and mitochondrial membrane potential of lung cells was studied by
Rhodamine123 fluorescence dye. Terminal deoxynucleotidyl transferase dUTP nick end
labeling (TUNEL) test was used to show DNA fragmentation and apoptosis of lung
cells. Also, the Rat inflammatory cytokine assay kit was used to measure the
concentrations of inflammatory cytokines. The catalyst-treated cell cutlers
significantly (p ≤ 0.01) reduced the DNA fragmentation, caspase-3 activity, and
inflammatory cytokines concentrations, and raised the mitochondrial membrane
potential and cell viability in the high concentration of α-Guttiferin-treated lung
MRC-5, CCD[sbnd]19Lu, WI-38, and BEAS-2B cells. The best result of lung protective
properties of catalyst against α-Guttiferin was seen in the high dose of catalyst
i.e., 4 μg. DPPH test revealed similar antioxidant potentials for catalyst and
butylated hydroxytoluene. The catalyst inhibited half of the DPPH molecules in the
concentration of 171 μg/mL. According to the above results, catalyst can be
administrated as a lung protective drug for the treatment of lung diseases after
approving in the clinical trial studies in humans. © 2020 Elsevier B.V.
AU - Han, Y.
AU - Gao, Y.
AU - Cao, X.
AU - Liu, S.
AU - Li, J.
DB - Scopus
DO - 10.1016/j.ijbiomac.2020.08.183
KW - 3drug
Ag nanoparticles
Chitosan-alginate
Indazolophthalazine-triones
Magnetic nanocomposite
α-Guttiferin
Alginates
Animals
Catalysis
Cell Line
Cell Survival
Chitosan
Cytokines
Humans
Indazoles
Lung
Magnetite Nanoparticles
Phthalazines
Rats
Silver
Umbelliferones
alginic acid
alpha guttiferin
caspase 3
chemical compound
chitosan
cresol
indazolo[2,1 b]phthalazine
interleukin 1alpha
interleukin 1beta
interleukin 6
magnetite
nanocomposite
rhodamine 123
silver nanoparticle
unclassified drug
cytokine
indazole derivative
magnetite nanoparticle
phthalazine derivative
silver
umbelliferone derivative
apoptosis
Article
BEAS-2B cell line
catalyst
cell viability
colorimetry
controlled study
cytokine release
DNA fragmentation
enzyme activity
heating
human
human cell
lung toxicity
MRC-5 cell line
one pot synthesis
physical chemistry
TUNEL assay
vibrating sample magnetometry
WI-38 cell line
X ray diffraction
animal
catalysis
cell line
cell survival
chemistry
cytology
drug effect
immunology
lung
metabolism
rat
M3 - Article
PY - 2020
SP - 2974-2986
ST - Ag NPs on chitosan-alginate coated magnetite for synthesis of indazolo[2,1-
b]phthalazines and human lung protective effects against α-Guttiferin
TI - Ag NPs on chitosan-alginate coated magnetite for synthesis of indazolo[2,1-
b]phthalazines and human lung protective effects against α-Guttiferin
85090020864&doi=10.1016%2fj.ijbiomac.2020.08.183&partnerID=40&md5=b7503dbbcd15c06b9
2dbf5ce7f4bca2b
VL - 164
ID - 5307
ER -
TY - JOUR
AB - In this study, two new binuclear silver(I)-tolfenamic acid complexes
including picoline derivative ligands, [Ag2(μ-tolf)2(2-pic)2] 1 and [Ag2(μ-
tolf)2(4-pic)2] 2, were synthesized and identified by elemental analysis, FT-IR, 1H
NMR and thermal analysis techniques. The in vitro cytotoxicity of the complexes was
investigated against human breast cancer cell lines by XTT, flow cytometry, enzyme
activity and western blot studies. The structures of 1 and 2 were clarified by
single-crystal X-ray diffraction determination. The data clearly indicated that the
Ag(I) ion is coordinated to one auxiliary ligand (2-pic or 4-pic), two tolfenamato
ligands and another Ag(I) ion, demonstrating a distorted tetrahedral geometry. The
tolfenamato ligands act as a bridging bidentate ligands. The chains are stabilized
by intramolecular Ag(I)⋯π and π···π interactions. The 1H NMR and thermal analyses
displayed the presence of picoline and tolfenamato ligands in the coordination
sphere and the purity of the complexes. Both complexes exhibited potent
cytotoxicity against cancer cell lines with higher selectivity than carboplatin.
Flow cytometry and enzyme activity studies indicated that both complexes caused an
increase in apoptosis, ROS and NO levels, cell cycle arrest, mitochondrial membrane
damage and caspase activity, as well as inhibiting PI3K/Akt phosphorylation and
modulating the oxidant/antioxidant balance system. Western blot analyses revealed
the up-regulation of bax, caspase-3, caspase-9 and p53 proteins, but the down-
regulation of bcl-2. These results demonstrate the vigorous apoptotic potential of
the novel Ag(I) complexes in human breast cancer MDA-MB-453 cells. © 2021 Elsevier
Ltd
AU - Altay, A.
AU - Caglar, S.
AU - Kubra Kagan Yeniceri, E.
AU - Caglar, B.
AU - Sibel Şahin, Z.
C7 - 115189
DB - Scopus
DO - 10.1016/j.poly.2021.115189
KW - Ag(I) complexes
Breast cancer
Flow cytometry
NSAIDs
Western blot
M3 - Article
PY - 2021
ST - Binuclear silver(I) complexes with the non-steroidal anti-inflammatory drug
tolfenamic acid: Synthesis, characterization, cytotoxic activity and evaluation of
cellular mechanism of action
T2 - Polyhedron
TI - Binuclear silver(I) complexes with the non-steroidal anti-inflammatory drug
85103936561&doi=10.1016%2fj.poly.2021.115189&partnerID=40&md5=581b03a33c312248bcd7d
d327b2185cb
VL - 202
ID - 5159
ER -
TY - JOUR
AB - In this study, two new binuclear silver(I)-tolfenamic acid complexes
including picoline derivative ligands, [Ag-2(mu-tolf)(2)(2-pic)(2)] 1 and [Ag-2(mu-
tolf)(2)(4-pic)(2)] 2, were synthesized and identified by elemental analysis, FT-
IR, 1H NMR and thermal analysis techniques. The in vitro cytotoxicity of the
complexes was investigated against human breast cancer cell lines by XTT, flow
cytometry, enzyme activity and western blot studies. The structures of 1 and 2 were
clarified by single-crystal X-ray diffraction determination. The data clearly
indicated that the Ag(I) ion is coordinated to one auxiliary ligand (2-pic or 4-
pic), two tolfenamato ligands and another Ag(I) ion, demonstrating a distorted
tetrahedral geometry. The tolfenamato ligands act as a bridging bidentate ligands.
The chains are stabilized by intramolecular Ag(I)...pi and pi....pi interactions.
The H-1 NMR and thermal analyses displayed the presence of picoline and tolfenamato
ligands in the coordination sphere and the purity of the complexes. Both complexes
exhibited potent cytotoxicity against cancer cell lines with higher selectivity
than carboplatin. Flow cytometry and enzyme activity studies indicated that both
complexes caused an increase in apoptosis, ROS and NO levels, cell cycle arrest,
mitochondrial membrane damage and caspase activity, as well as inhibiting PI3K/Akt
phosphorylation and modulating the oxidant/antioxidant balance system. Western blot
analyses revealed the up-regulation of bax, caspase-3, caspase-9 and p53 proteins,
but the down-regulation of bcl-2. These results demonstrate the vigorous apoptotic
potential of the novel Ag(I) complexes in human breast cancer MDA-MB-453 cells. (C)
2021 Elsevier Ltd. All rights reserved.
AN - WOS:000652846800005
AU - Altay, A.
AU - Caglar, S.
AU - Yeniceri, E. K. K.
AU - Caglar, B.
AU - Sahin, Z. S.
C6 - APR 2021
C7 - 115189
DA - JUL 1
DO - 10.1016/j.poly.2021.115189
PY - 2021
SN - 0277-5387
1873-3719
ST - Binuclear silver(I) complexes with the non-steroidal anti-inflammatory drug
T2 - POLYHEDRON
TI - Binuclear silver(I) complexes with the non-steroidal anti-inflammatory drug
VL - 202
ID - 6249
ER -
TY - JOUR
AB - Hepatocellular carcinoma is one of the most common lethal diseases worldwide
and there is no effective treatment till date. Natural products derived from the
plants play an important role in chemoprevention and act as therapeutic antitumor
agents. Licorice is a plant that has been used in food and medicine for the
treatment of various diseases. 18 beta-Glycyrrhetinic acid (18 beta-GA), a
pentacyclic triterpenoid obtained from the roots of licorice plant, is reported to
possess various pharmacological properties such as antitumor and antiinflammatory
activities. The present study was designed to elucidate the chemopreventive effect
of 18 beta-GA through antiinflammation, antiproliferation, and induction of
apoptosis in human hepatoma cell line HepG2. 18 beta-GA significantly inhibits the
proliferation of HepG2 cell without affecting the normal liver cell line (Chang's).
In the present study, 18 beta-GA increased the formation of reactive oxygen
species, nitric oxide production, and loss of mitochondrial membrane potential,
suggesting the involvement of 18 beta-GA in apoptosis which was also confirmed by
assessing the markers involved in apoptosis like caspase-3, caspase-9, Bax:Bcl-2
ratio, and cleaved PARP. 18 beta-GA also downregulated the expression of
inflammatory proteins such as NF-kappa B, iNOS, and COX-2. Keeping these data into
consideration, our results suggest that 18 beta-GA may be used as a chemopreventive
agent in liver cancer.
AN - WOS:000377592800015
AU - Hasan, S. K.
AU - Siddiqi, A.
AU - Nafees, S.
AU - Ali, N.
AU - Rashid, S.
AU - Ali, R.
AU - Shahid, A.
AU - Sultana, S.
DA - MAY
DO - 10.1007/s11010-016-2705-2
IS - 1-2
PY - 2016
SN - 0300-8177
1573-4919
SP - 169-177
ST - Chemopreventive effect of 18 beta-glycyrrhetinic acid via modulation of
inflammatory markers and induction of apoptosis in human hepatoma cell line (HepG2)
T2 - MOLECULAR AND CELLULAR BIOCHEMISTRY
TI - Chemopreventive effect of 18 beta-glycyrrhetinic acid via modulation of
inflammatory markers and induction of apoptosis in human hepatoma cell line (HepG2)
VL - 416
ID - 6355
ER -
TY - JOUR
AB - Liver and kidney diseases are the most frequently encountered problems around
the globe. Damage to the liver and kidney may occur as a result of exposure to
various drugs, chemicals, toxins, and pathogens, leading to severe disease
conditions such as cirrhosis, fibrosis, hepatitis, acute kidney injury, and liver
and renal failure. In this regard, the use of nanoparticles (NPs) such as silver
nanoparticles (AgNPs), gold nanoparticles (AuNPs), and zinc oxide nanoparticles
(ZnONPs) has emerged as a rapidly developing field of study in terms of safe
delivery of various medications to target organs with minimal side effects. Due to
their physical characteristics, NPs have inherent pharmacological effects, and an
accidental buildup can have a significant impact on the structure and function of
the liver and kidney. By suppressing the expression of the proinflammatory
cytokines iNOS and COX-2, NPs are known to possess anti-inflammatory effects.
Additionally, NPs have demonstrated their ability to operate as an antioxidant,
squelching the generation of ROS caused by substances that cause oxidative stress.
Finally, because of their pro-oxidant properties, they are also known to increase
the level of ROS, which causes malignant liver and kidney cells to undergo
apoptosis. As a result, NPs can be regarded as a double-edged sword whose inherent
therapeutic benefits can be refined as we work to comprehend them in terms of their
toxicity. © 2022 by the authors.
AU - Hashim, M.
AU - Mujahid, H.
AU - Hassan, S.
AU - Bukhari, S.
AU - Anjum, I.
AU - Hano, C.
AU - Abbasi, B. H.
AU - Anjum, S.
C7 - 1337
DB - Scopus
DO - 10.3390/biom12101337
IS - 10
antioxidant
kidney
liver
nanoparticles
pro-oxidant
toxicity
Antioxidants
Cyclooxygenase 2
Cytokines
Gold
Humans
Kidney Diseases
Liver
Metal Nanoparticles
Nanoparticles
Oxidative Stress
Silver
Zinc Oxide
alloxan
azathioprine
cadmium chloride
carbon tetrachloride
chitosan
cisplatin
corticosteroid
cyclooxygenase 2
cyclosporine
cytokine
diethylnitrosamine
dimethylnitrosamine
entecavir
gold nanoparticle
interleukin 12
lead
methotrexate
nanoparticle
nitric oxide
ochratoxin
paracetamol
protein p53
silver nanoparticle
sirolimus
streptozocin
tenofovir
thioacetamide
gold
metal nanoparticle
silver
zinc oxide
anorexia
apoptosis
cardiovascular disease
chronic kidney failure
chronic liver disease
diabetes mellitus
hematologic disease
Hep-G2 cell line
human
human cell
hypertension
hypothyroidism
jaundice
kidney cell
kidney disease
kidney injury
Kupffer cell
liver cell
liver injury
MTT assay
nephrotoxicity
oxidative stress
Review
seizure
toxicity testing
wound healing
wound infection
chemistry
metabolism
M3 - Review
PY - 2022
ST - Implication of Nanoparticles to Combat Chronic Liver and Kidney Diseases:
Progress and Perspectives
T2 - Biomolecules
TI - Implication of Nanoparticles to Combat Chronic Liver and Kidney Diseases:
Progress and Perspectives
85140441320&doi=10.3390%2fbiom12101337&partnerID=40&md5=7a579e9b5622b1c299e3ecb5e6f
32c9d
VL - 12
ID - 5108
ER -
TY - JOUR
AB - Nanoparticles (NPs) are currently the focus of considerable attention for
dental applications; however, their biological effects have not been fully
elucidated. The long-term, slow release of matrix metalloproteases (MMPs) digests
collagen fibrils within resin-dentin bonds. Therefore, MMP inhibitors can prolong
the durability of resin-dentin bonds. However, there have been few reports
evaluating the combined effect of MMP inhibition and the cytotoxic effects of NPs
for dentin bonding. The aim of this study was to evaluate MMP inhibition and
cytotoxic responses to gold (AuNPs) and platinum nanoparticles (PtNPs) stabilized
by polyvinylpyrrolidone (PVP) in cultured murine macrophages (RAW264) by using MMP
inhibition assays, measuring cell viability and inflammatory responses
(quantitative reverse transcription polymerase chain reaction [RT-qPCR]), and
conducting a micromorphological analysis by fluorescence and transmission electron
microscopy. Cultured RAW264 cells were exposed to metal NPs at various
concentrations (1, 10, 100, and 400 mu g/mL). AuNPs and PtNPs markedly inhibited
MMP-8 and MMP-9 activity. Although PtNPs were cytotoxic at high concentrations (100
and 400 mu g/mL), no cytotoxic effects were observed for AuNPs at any
concentration. Transmission electron microscopy images showed a significant
nonrandom intercellular distribution for AuNPs and PtNPs, which were mostly
observed to be localized in lysosomes but not in the nucleus. RT-qPCR analysis
demonstrated inflammatory responses were not induced in RAW264 cells by AuNPs or
PtNPs. The cytotoxicity of nanoparticles might depend on the core metal composition
and arise from a "Trojan horse" effect; thus, MMP inhibition could be attributed to
the surface charge of PVP, which forms the outer coating of NPs. The negative
charge of the surface coating of PVP binds to Zn2+ from the active center of MMPs
by chelate binding and results in MMP inhibition. In summary, AuNPs are attractive
NPs that effectively inhibit MMP activity without cytotoxicity or inflammatory
responses.
AN - WOS:000358182000010
AU - Hashimoto, M.
AU - Sasaki, J. I.
AU - Yamaguchi, S.
AU - Kawai, K.
AU - Kawakami, H.
AU - Iwasaki, Y.
AU - Imazato, S.
DA - AUG
DO - 10.1177/0022034515589282
IS - 8
PY - 2015
SN - 0022-0345
1544-0591
SP - 1085-1091
ST - Gold Nanoparticles Inhibit Matrix Metalloproteases without Cytotoxicity
T2 - JOURNAL OF DENTAL RESEARCH
TI - Gold Nanoparticles Inhibit Matrix Metalloproteases without Cytotoxicity
VL - 94
ID - 6311
ER -
TY - JOUR
AB - Nanometals are currently receiving considerable attention for industrial and
biomedical applications, but their potentially hazardous and toxic effects have not
been extensively studied. This study evaluated the biological responses of novel
water-dispersible gold (Au-NPs) and silver nanoparticles (Ag-NPs) stabilized by Au-
C or Ag-C sigma-bonds in cultured macrophages (RAW264.7), via analysis of the cell
viability, the integrity of the plasma membrane, and the inflammatory and
morphological properties. The cultured RAW264.7 was exposed to metal-NPs at various
concentrations. The Ag-NPs showed cytotoxicity at high NP concentrations, but the
cytotoxic effects of the Au-NPs were smaller than those of the Ag-NPs. For the
microscopic analysis, both types of particles were internalized into cells, the
morphological changes in the cells which manifested as an expansion of the
vesicles' volume, were smaller for the Au-NPs compared with the Ag-NPs. For the Ag-
NPs, the endocytosis abilities of the macrophages might have induced harmful
effects, because of the expansion of the cell vesicles. Although an inflammatory
response was observed for both the Au- and Ag-NPs, the harmful effects of the Au-
NPs were smaller than those of the Ag-NPs, with minor morphological changes
observed even after internalization of the NPs into the cells. (c) 2013 Wiley
Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1838-1849, 2014.
AN - WOS:000334488800022
AU - Hashimoto, M.
AU - Toshima, H.
AU - Yonezawa, T.
AU - Kawai, K.
AU - Narushima, T.
AU - Kaga, M.
AU - Endo, K.
DA - JUN
DO - 10.1002/jbm.a.34854
IS - 6
PY - 2014
SN - 1549-3296
1552-4965
SP - 1838-1849
ST - Responses of RAW264.7 macrophages to water-dispersible gold and silver
nanoparticles stabilized by metal-carbon sigma-bonds
T2 - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
TI - Responses of RAW264.7 macrophages to water-dispersible gold and silver
nanoparticles stabilized by metal-carbon sigma-bonds
VL - 102
ID - 6102
ER -
TY - JOUR
AB - Nanometals are currently receiving considerable attention for industrial and
biomedical applications, but their potentially hazardous and toxic effects have not
been extensively studied. This study evaluated the biological responses of novel
water-dispersible gold (Au-NPs) and silver nanoparticles (Ag-NPs) stabilized by Au-
C or Ag-C σ-bonds in cultured macrophages (RAW264.7), via analysis of the cell
viability, the integrity of the plasma membrane, and the inflammatory and
morphological properties. The cultured RAW264.7 was exposed to metal-NPs at various
concentrations. The Ag-NPs showed cytotoxicity at high NP concentrations, but the
cytotoxic effects of the Au-NPs were smaller than those of the Ag-NPs. For the
microscopic analysis, both types of particles were internalized into cells, the
morphological changes in the cells which manifested as an expansion of the
vesicles' volume, were smaller for the Au-NPs compared with the Ag-NPs. For the Ag-
NPs, the endocytosis abilities of the macrophages might have induced harmful
effects, because of the expansion of the cell vesicles. Although an inflammatory
response was observed for both the Au- and Ag-NPs, the harmful effects of the Au-
NPs were smaller than those of the Ag-NPs, with minor morphological changes
observed even after internalization of the NPs into the cells. © 2013 Society of
Plastics Engineers.
AU - Hashimoto, M.
AU - Toshima, H.
AU - Yonezawa, T.
AU - Kawai, K.
AU - Narushima, T.
AU - Kaga, M.
AU - Endo, K.
DB - Scopus
DO - 10.1002/jbm.a.34854
IS - 6
KW - endocytosis
gold
macrophage
nanoparticle
silver
Animals
Carbon
Cell Line
Cell Survival
Gold
Macrophages
Mice
Nanoparticles
Silver
Solubility
Water
Cell membranes
Cytology
Cytotoxicity
Molecular biology
Morphology
carbon
gold nanoparticle
metal
silver nanoparticle
water
Microscopic analysis
Morphological changes
Morphological properties
animal cell
cell culture
cell membrane
cell structure
cell vacuole
cell viability
chemical bond
cytotoxicity
dispersion
enzyme release
inflammation
molecular stability
nonhuman
review
animal
cell line
cell survival
chemistry
cytology
drug effects
immunology
metabolism
mouse
solubility
M3 - Review
PY - 2014
SP - 1838-1849
ST - Responses of RAW264.7 macrophages to water-dispersible gold and silver
nanoparticles stabilized by metal-carbon σ-bonds
T2 - Journal of Biomedical Materials Research - Part A
TI - Responses of RAW264.7 macrophages to water-dispersible gold and silver
nanoparticles stabilized by metal-carbon σ-bonds
84899409054&doi=10.1002%2fjbm.a.34854&partnerID=40&md5=a5997f1a60452ea46b40eef0b152
a4a0
VL - 102
ID - 5650
ER -
TY - JOUR
AB - This study evaluated the inhibition of matrix metalloproteases (MMPs) and
cellular responses elicited by gold (Au) and platinum (Pt) nanoparticles (NPs). The
interaction of MMP-1 and NPs was evaluated using an MMP assay kit. The cultured
L929 cells were exposed to various concentrations of NPs. The cellular responses to
NPs were examined using a cytotoxicity assay (that evaluated cell viability and
lactic dehydrogenase production), real-time polymerase chain reaction (RT-qPCR),
and transmission electron microscopy. Both types of NPs, when used at
concentrations above 10 g ml(-1), inhibited MMP-1 activity. No cytotoxic effects
were found when the cells were exposed to AuNPs. In contrast, PtNPs, at both 100
and 400 g ml(-1), induced cytotoxicity. No inflammatory responses (production of
interleukin-6 and tumor necrosis factor-alpha) to NPs were identified by RT-qPCR.
The negative surface charge of NPs (COOH-) binds to the Zn2+ of the MMP active
center by chelation, leading to MMP inhibition. Gold nanoparticles are plausible
candidates for MMP inhibitors in resin-bonding materials because they effectively
inhibit MMP-1 activity without cytotoxic or inflammatory effects.
AN - WOS:000367956900011
AU - Hashimoto, M.
AU - Yamaguchi, S.
AU - Sasaki, J. I.
AU - Kawai, K.
AU - Kawakami, H.
AU - Iwasaki, Y.
AU - Imazato, S.
DA - FEB
DO - 10.1111/eos.12235
IS - 1
PY - 2016
SN - 0909-8836
1600-0722
SP - 68-74
ST - Inhibition of matrix metalloproteinases and toxicity of gold and platinum
nanoparticles in L929 fibroblast cells
T2 - EUROPEAN JOURNAL OF ORAL SCIENCES
TI - Inhibition of matrix metalloproteinases and toxicity of gold and platinum
nanoparticles in L929 fibroblast cells
VL - 124
ID - 6312
ER -
TY - JOUR
AB - Objectives The first goal of this study was to synthesize the silver
nanoparticles Alcaligenes xylosoxidans exopolysaccharide (Ag-AXEPS). The second
objective was to analyse the role of Ag-AXEPS nanoparticles (NPS) in treating
bleomycin (BLM)-induced lung fibrosis. Methods Intratracheal bleomycin (2.5 U/kg)
was administered to prompt pulmonary fibrosis in rats, and pulmonary fibrosis was
treated with Ag-AXEPS nanoparticles (100 ppm/twice a week for four weeks). Key
findings Ag-AXEPS nanoparticles significantly decreased the diversity of pulmonary
inflammatory agents in rats with BLM-induced fibrosis. Reduced levels of
respiratory tumor necrosis factor-alpha, monocyte chemotactic protein-1, matrix
metalloproteinases (MMP-2 and MMP-9) were observed on treatment with synthesized
Ag-AXEPS. Similarly, the treatment decreased IL-12, mRNA levels of BAX and plasma
fibrosis markers like N-terminal procollagen III propeptide and transforming growth
factor-β1. On the other hand, the treatment increased mRNA BCL2 and total
antioxidant capacity. It also lowered the level of fibrosis, as was shown by a
quantified pathologic study of hematoxylin–eosin-stained lung parts.The treatment,
however, ensured that lung collagen was restored, as assessed by Masson’s trichrome
stain, and that overall survival was increased and enhanced. Conclusions: Our work
showed that nanoparticles could be obtained at 37°C and may be a possible pulmonary
fibrosis therapeutic agent. © The Author(s) 2021. Published by Oxford University
Press on behalf of the Royal Pharmaceutical Society. All rights reserved.
AU - Hassan, A. I.
AU - Samir, A.
AU - Youssef, H. F.
AU - Mohamed, S. S.
AU - Asker, M. S.
AU - Mahmoud, M. G.
DB - Scopus
DO - 10.1093/jpp/rgab037
IS - 11
KW - apoptosis
exopolysaccharides
Masson’s trichrome
matrix metalloproteinases
pulmonary fibrosis
Alcaligenes
Animals
Antibiotics, Antineoplastic
Antioxidants
Apoptosis
Bleomycin
Collagen
Fibrosis
Inflammation
Lung
Male
Matrix Metalloproteinases
Metal Nanoparticles
Nanoconjugates
Nanoparticles
Pneumonia
Polysaccharides
Polysaccharides, Bacterial
Pulmonary Fibrosis
Silver
Transforming Growth Factor beta1
bleomycin
collagen
eosin
exopolysaccharide
gelatinase A
gelatinase B
hematoxylin
interleukin 12
messenger RNA
nanocarrier
procollagen type 3 aminopropeptide
protein Bax
protein bcl 2
respiratory tract agent
silver nanoparticle
antineoplastic antibiotic
antioxidant
bacterial polysaccharide
matrix metalloproteinase
metal nanoparticle
nanoconjugate
nanoparticle
polysaccharide
silver
Tgfb1 protein, rat
Achromobacter xylosoxidans
adult
animal experiment
animal model
animal tissue
Article
bleomycin-induced pulmonary fibrosis
controlled study
drug effect
lung fibrosis
male
Masson staining
nonhuman
overall survival
rat
synthesis
treatment duration
animal
fibrosis
inflammation
lung
metabolism
pathology
pneumonia
Sprague Dawley rat
M3 - Article
PY - 2021
SP - 1503-1512
ST - Effects of silver nanoparticles–polysaccharide on bleomycin-induced pulmonary
fibrosis in rats
T2 - Journal of Pharmacy and Pharmacology
TI - Effects of silver nanoparticles–polysaccharide on bleomycin-induced pulmonary
fibrosis in rats
85118096853&doi=10.1093%2fjpp
%2frgab037&partnerID=40&md5=1627596c4e9794f645c28499fb092a5b
VL - 73
ID - 5246
ER -
TY - JOUR
AB - Detoxification is one of the main vital tasks performed by the liver. The
purpose of this study was to investigate whether mustard in its normal or
nanoparticles could confer a protective/therapeutic effect against TAA-induced
acute liver failure in experimental animal models. Mustard ethanolic extract was
analyzed by HPLC/MS. To induce liver failure, male rats were injected with 350
mg/kg bw TAA IP, then treated orally with a dose of 100 mg/kg for 15 d of mustard
extract and its nanoform before and following induction. The levels of serum liver
functions, total cholesterol (TCHo), total glyceride (TG), total bilirubin (TBIL),
hepatic malonaldhyde (MDA) and nitric oxide (NO),glutathione (GSH), sodium oxide
dismutase (SOD), as well as tumor necrosis factor (TNF-alpha,) and interleukin 6
(IL-6), were estimated. DNA genotoxicity and hepatic pathology, and
immunohistologic (IHC) changes were assayed. The antioxidant content of Phenolic
acids, flavonoids in mustard ethanolic extract substantially decreased the levels
of ALT, AST, ALP and rehabilitated the histopathological alterations. In addition,
nanoforms of mustard ethanol extract have notably increased the levels of GSH, SOD
and significantly reduced the levels of MDA. The expression levels of TNF-alpha and
IL-6 in serum and tissue were markedly downregulated. DNA genotoxicity was
significantly reversed. Mustard introduced a protective and medicinal effect
against TAA in both its forms.
AN - WOS:000601755000001
AU - Hassan, S. A.
AU - El Hagrassi, A. M.
AU - Hammam, O.
AU - Soliman, A. M.
AU - Ezzeldin, E.
AU - Aziz, W. M.
C7 - 1650
DA - DEC
DO - 10.3390/biom10121650
IS - 12
PY - 2020
SN - 2218-273X
ST - Brassica juncea L. (Mustard) Extract Silver NanoParticles and Knocking off
Oxidative Stress, ProInflammatory Cytokine and Reverse DNA Genotoxicity
T2 - BIOMOLECULES
TI - Brassica juncea L. (Mustard) Extract Silver NanoParticles and Knocking off
Oxidative Stress, ProInflammatory Cytokine and Reverse DNA Genotoxicity
VL - 10
ID - 6345
ER -
TY - JOUR
AB - Detoxification is one of the main vital tasks performed by the liver. The
purpose of this study was to investigate whether mustard in its normal or
nanoparticles could confer a protective/therapeutic effect against TAA-induced
acute liver failure in experimental animal models. Mustard ethanolic extract was
analyzed by HPLC/MS. To induce liver failure, male rats were injected with 350
mg/kg bw TAA IP, then treated orally with a dose of 100 mg/kg for 15 d of mustard
extract and its nanoform before and following induction. The levels of serum liver
functions, total cholesterol (TCHo), total glyceride (TG), total bilirubin (TBIL),
hepatic malonaldhyde (MDA) and nitric oxide (NO),glutathione (GSH), sodium oxide
dismutase (SOD), as well as tumor necrosis factor (TNF-α,) and interleukin 6 (IL-
6), were estimated. DNA genotoxicity and hepatic pathology, and immunohistologic
(IHC) changes were assayed. The antioxidant content of Phenolic acids, flavonoids
in mustard ethanolic extract substantially decreased the levels of ALT, AST, ALP
and rehabilitated the histopathological alterations. In addition, nanoforms of
mustard ethanol extract have notably increased the levels of GSH, SOD and
significantly reduced the levels of MDA. The expression levels of TNF-α and IL-6 in
serum and tissue were markedly downregulated. DNA genotoxicity was significantly
reversed. Mustard introduced a protective and medicinal effect against TAA in both
its forms. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Hassan, S. A.
AU - Hagrassi, A. M. E.
AU - Hammam, O.
AU - Soliman, A. M.
AU - Ezzeldin, E.
AU - Aziz, W. M.
C7 - 1650
DB - Scopus
DO - 10.3390/biom10121650
IS - 12
KW - Acute liver toxicity
Brassica juncea L. seeds extract
DNA genotoxicity
LC/MS
Phenolic compounds
Pro-inflammatory cytokine
Thioacetamide (TAA)
Animals
Antioxidants
Bilirubin
Cholesterol
DNA Damage
Drug Administration Schedule
Glutathione
Interleukin-6
Liver
Male
Malondialdehyde
Metal Nanoparticles
Mustard Plant
Nitric Oxide
Oxidative Stress
Plant Extracts
Rats
Rats, Wistar
Silver
Thioacetamide
Triglycerides
antioxidant
bilirubin
Brassica juncea extract
catalase
cholesterol
essential oil
flavonoid
glutathione
interleukin 6
malonaldehyde
nitric oxide
phenol derivative
plant extract
quercetin
silver nanoparticle
terpenoid
triacylglycerol
unclassified drug
Il6 protein, rat
metal nanoparticle
silver
thioacetamide
animal experiment
animal model
animal tissue
Article
controlled study
DNA damage
drug synthesis
gene expression
genotoxicity
histopathology
lipid peroxidation
liver injury
mass spectrometry
nonhuman
oxidative stress
phytochemistry
protein expression
rat
thioacetamide-induced liver injury
animal
blood
chemistry
drug administration
drug effect
genetics
liver
male
metabolism
mustard
pathology
toxic hepatitis
Wistar rat
M3 - Article
PY - 2020
SP - 1-19
ST - Brassica juncea l. (mustard) extract silver nanoparticles and knocking off
oxidative stress, proinflammatory cytokine and reverse dna genotoxicity
T2 - Biomolecules
TI - Brassica juncea l. (mustard) extract silver nanoparticles and knocking off
oxidative stress, proinflammatory cytokine and reverse dna genotoxicity
85097418514&doi=10.3390%2fbiom10121650&partnerID=40&md5=f021a57dd0002afbf72ca83dc24
f17a3
VL - 10
ID - 5286
ER -
TY - JOUR
AB - Background: Pomegranate (Punica granatum L) has been used since ancient times
in the traditional medicine of several cultures, particularly in the Middle East.
It is an essential commercial crop full of bioactive compounds with several medical
applications. Pomegranate is very popular for its biological effects exerted by
phenolic compounds via free radical scavenging abilities. It has revealed high
antioxidant and anti-inflammatory activities and is beneficial for the amelioration
of liver and kidney diseases. Purpose: To elucidate the potential efficacy of
pomegranate juice (PJ) against copper oxide nanoparticles (CuO-NPs)-induced
apoptosis, inflammation, and oxidative stress damage. Study design: 37 nm sized
CuO-NPs were prepared by precipitation method and characterized by using X-ray
diffractometer (XRD), Zetasizer nano-and high-resolution transmission electron
microscope (HR-TEM). 30 Wistar rats were partitioned into 6 equal groups as
follows: Group 1 (negative control), groups 2 & 3 (PJ control groups), group 4
(CuO-NPs group), groups 5 & 6 (CuO-NPs + PJ groups). Methods: Hepato-renal
protective effect of PJ was evaluated by measuring levels of serum marker enzymes
(ALT, AST,blood urea nitrogen and creatinine). Cu NPs bioaccumulation in liver and
kidneys was determined by using atomic absorption spectrophotometer. The oxidative
stress markers, Rt-PCR analysis, histopathological and immunohistochemical studies
were carried out in the liver and kidneys to support the above parameters. Results:
Rats injected with CuO-NPs showed higher levels of the above serum marker enzymes,
alteration of oxidant-antioxidant balance together with severe pathological
alterations in liver and kidney tissues and overexpression of both caspase-3 and
nuclear factor kappa B protein (NF-kappa B) associated with upregulation of Box
gene and downregulation of Bcl2 gene in these organs. PJ ameliorated all of the
above toxicological parameters. Conclusion: PJ was proved to be a potential hepato-
renal protective agent against liver and kidney damage induced by CuO-NPs via its
antioxidant, anti-inflammatory, and anti-apoptotic effects.
AN - WOS:000496719300001
AU - Hassanen, E. I.
AU - Tohamy, A. F.
AU - Issa, M. Y.
AU - Ibrahim, M. A.
AU - Farroh, K. Y.
AU - Hassan, A. M.
DO - 10.2147/IJN.S229461
PY - 2019
SN - 1178-2013
SP - 8905-8922
ST - Pomegranate Juice Diminishes The Mitochondria-Dependent Cell Death And NF-kB
Signaling Pathway Induced By Copper Oxide Nanoparticles On Liver And Kidneys Of
Rats
TI - Pomegranate Juice Diminishes The Mitochondria-Dependent Cell Death And NF-kB
Signaling Pathway Induced By Copper Oxide Nanoparticles On Liver And Kidneys Of
Rats
VL - 14
ID - 6389
ER -
TY - JOUR
AB - The green synthesis of silver nanoparticles (Ag NPs) for catalysis and
biological applications has gained great interest. Natural elm pods are a type of
food that possesses anti-inflammatory and pain-relieving effects. In this study,
elm pod polysaccharide (EPP) was extracted from elm pods using hot water extraction
for the first time. Biocompatible EPP-stabilized silver nanoparticles (EPP-Agn NPs)
were prepared by using a green synthesis method. The EPP-Ag25 NPs had a
hydrodynamic size of 40.9 nm and a highly negative surface charge of −27.4 mV.
Furthermore, EPP-Ag25 NPs exhibited high catalytic activity for the reduction of 4-
nitrophenol, and the catalytic reaction followed a pseudo-first order kinetic
equation. More importantly, the inhibition rate of EPP-Ag25 NPs on Escherichia coli
was 71 % when samples were treated with an 808 nm laser. Besides, EPP-Agn NPs
effectively inhibited the proliferation of tumor cells irradiated by an 808 nm
laser. The improved performance of EPP-Agn NPs was due to the good stability of
EPP. Taken together, EPP-Agn NPs had good stability, catalytic activity,
antibacterial and antitumor ability under laser irradiation. EPP is a good
stabilizer for many nanoparticles which have broad applications in the field of
catalysis and biomedicine in the future. © 2022 Elsevier B.V.
AU - He, M.
AU - Han, Z.
AU - Liang, Y.
AU - Zhao, H.
AU - Ji, X.
AU - Ma, G.
AU - Cui, Y.
AU - Wang, L.
DB - Scopus
DO - 10.1016/j.ijbiomac.2022.06.025
KW - Catalysis
Elm pod polysaccharide
Escherichia coli
Metal Nanoparticles
Polysaccharides
Silver
4 nitrophenol
antiinfective agent
elm pod polysaccharide
polysaccharide
silver nanoparticle
stabilizing agent
unclassified drug
water
metal nanoparticle
silver
Article
bacteriostasis
biocompatibility
biomedicine
catalysis
cell proliferation
cell viability
controlled study
extraction
green chemistry
HeLa cell line
human
human cell
hydrodynamics
kinetics
molecular stability
nonhuman
particle size
performance
pseudo first order kinetic equation
surface charge
tumor cell
M3 - Article
PY - 2022
SP - 1078-1087
ST - Green synthesis of Ag nanoparticles using elm pod polysaccharide for
catalysis and bacteriostasis
TI - Green synthesis of Ag nanoparticles using elm pod polysaccharide for
catalysis and bacteriostasis
85131964056&doi=10.1016%2fj.ijbiomac.2022.06.025&partnerID=40&md5=6ac15c01078fdbadb
2e934d1e292b91a
VL - 213
ID - 5059
ER -
TY - JOUR
AB - Brucella spp. are facultative intracellular bacteria that cause brucellosis
in humans and other animals. Brucella spp. are taken up by macrophages, and the
outcome of the macrophage-Brucella interaction is a basis for establishment of a
chronic Brucella infection. Microarrays were used to analyze the transcriptional
response of the murine macrophage-like J774.A1 cell line to infection with virulent
Brucella melitensis strain 16M. It was found that most significant changes in
macrophage gene transcription happened early following infection, and global
macrophage gene expression profiles returned to normal between 24 and 48 h
postinfection. These findings support the observation that macrophages kill the
majority of Brucella cells at the early infection stage, but the surviving Brucella
cells are able to avoid macrophage brucellacidal activity inside replicative
phagosomes at the later infection stage. At 4 h postinfection, macrophage genes
involved in cell growth, metabolism, and responses to endogenous stimuli were down-
regulated, while the inflammatory response (e.g., tumor necrosis factor alpha and
Toll-like receptor 2), the complement system, the responses to external stimuli,
and other immune responses were up-regulated. It is likely that the most active
brucellacidal activity happened between 0 and 4 111 postinfection. Mitochondrion-
associated gene expression, which is involved in protein synthesis and transport,
electron transfer, and small-molecule transfer, and many other mitochondrial
functions were significantly down-regulated at 4 h postinfection. Although there
were both pro- and antiapoptosis effects, B. melitensis 16M appears to inhibit
apoptosis of macrophages by blocking release of cytochrome c and production of
reactive oxygen species in the mitochondria, thus preventing activation of caspase
cascades.
AN - WOS:000240296400009
AU - He, Y. Q.
AU - Reichow, S.
AU - Ramamoorthy, S.
AU - Ding, X. C.
AU - Lathigra, R.
AU - Craig, J. C.
AU - Sobral, B. W. S.
AU - Schurig, G. G.
AU - Sriranganathan, N.
AU - Boyle, S. M.
DA - SEP
DO - 10.1128/IAI.01998-05
IS - 9
PY - 2006
SN - 0019-9567
1098-5522
SP - 5035-5046
ST - Brucella melitensis triggers time-dependent modulation of a apoptosis and
down-regulation of mitochondrion-associated gene expression in mouse macrophages
TI - Brucella melitensis triggers time-dependent modulation of a apoptosis and
down-regulation of mitochondrion-associated gene expression in mouse macrophages
VL - 74
ID - 6660
ER -
TY - JOUR
AB - Rationale: Humans with a dominant negative mutation in STAT3 are susceptible
to severe skin infections, suggesting an essential role for STAT3 signaling in
defense against cutaneous pathogens. Methods: To focus on innate antiviral defenses
in keratinocytes, we used a standard model of cutaneous infection of severe
combined immunodeficient mice with the current smallpox vaccine, ACAM-2000. In
parallel, early events post-infection with the smallpox vaccine ACAM-2000 were
investigated in cultured keratinocytes of human and mouse origin. Results: Mice
treated topically with a STAT3 inhibitor (Stattic) developed larger vaccinia
lesions with higher virus titers and died more rapidly than untreated controls.
Cultured human and murine keratinocytes infected with ACAM-2000 underwent rapid
necrosis, but when treated with Stattic or with inhibitors of RIP1 kinase or
caspase-1, they survived longer, produced higher titers of virus, and showed
reduced activation of type I interferon responses and inflammatory cytokines
release. Treatment with inhibitors of RIP1 kinase and STAT3, but not caspase-1,
also reduced the inflammatory response of keratinocytes to TLR ligands. Vaccinia
growth properties in Vero cells, which are known to be defective in some antiviral
responses, were unaffected by inhibition of RIP1K, caspase-1, or STAT3.
Conclusions: Our findings indicate that keratinocytes suppress the replication and
spread of vaccinia virus by undergoing rapid programmed cell death, in a process
requiring STAT3. These data offer a new framework for understanding susceptibility
to skin infection in patients with STAT3 mutations. Interventions which promote
prompt necroptosis/pyroptosis of infected keratinocytes may reduce risks associated
with vaccination with live vaccinia virus.
AN - WOS:000346766900066
AU - He, Y.
AU - Fisher, R.
AU - Chowdhury, S.
AU - Sultana, I.
AU - Pereira, C. P.
AU - Bray, M.
AU - Reed, J. L.
C7 - e113690
DA - NOV 24
DO - 10.1371/journal.pone.0113690
IS - 11
PY - 2014
SN - 1932-6203
ST - Vaccinia Virus Induces Rapid Necrosis in Keratinocytes by a STAT3-Dependent
Mechanism
T2 - PLOS ONE
TI - Vaccinia Virus Induces Rapid Necrosis in Keratinocytes by a STAT3-Dependent
Mechanism
VL - 9
ID - 6821
ER -
TY - JOUR
AB - Recently, the development of skin barrier depend on wound healing, which is
one of the most complicated biological processes. As an alternative to conventional
antibiotics, nanoparticles (NPs) have become more utilized generally to attack
bacteria. Due to their distinct characteristics, potential microbicidal action, and
ability to speed up the wound healing process, zinc oxide nanoparticles (ZnO-NPs)
have attracted much attention. Biological techniques can solve the restrictions of
both physical and chemical approaches for nanoparticles synthesis. Because it does
not require expensive chemicals, high temperatures, or a lot of time, biological
synthesis is relatively easy, inexpensive, and environmentally benign. The
secondary metabolic extract from Escherichia coli was used in this study to
biologically synthesize three distinct quantities of ZnO-NPs, which were then
assessed for their effectiveness in wound healing and bacterial infection
prevention. The biofabricated ZnO-NPs were fully characterized in terms of particle
shape, morphology, and stability against aggregation. Depending on the
concentration of the utilized zinc salt, three different samples were fabricated
biologically, nominated as ZnO-NPs-1, ZnO-NPs-2, and ZnO-NPs-3. The findings of Uv-
vis absorption peaks were obtained at 352 nm, demonstrating the preparation of ZnO-
NPs. The results demonstrated the formation of ZnO-NPs with an average particle
size of 79.19, 79.83 and 91.57 nm for the three prepared samples (ZnO-NPs-1, ZnO-
NPs-2, and ZnO-NPs-3), respectively. Additionally, these samples of ZnO-NPs
exhibited zeta potential values around -34.3, -33.7, and -33.4 mV, respectively.
Energy dispersive X-ray confirmed the successful formation of ZnO-NPs. It was also
observed from the obtained results that, ZnO-NP-3 showed superior antimicrobial
potential against selected skin infectious microbes. The effective killing dosage
of ZnO-NPs-3 was recorded to be 40 mg/L which can eliminate microbial growth. The
dysregulation of skin flora significantly influences the etiology of inflammatory
skin disorders.
AN - WOS:000997003000004
AU - Hemdan, B. A.
AU - El-Naggar, M. E.
AU - Abd-Elgawad, S. E.
AU - El Zawawy, N. A.
AU - Mahmoud, Y. A. G.
C6 - MAY 2023
DA - 2023 MAY 29
DO - 10.1007/s13399-023-04313-7
PY - 2023
SN - 2190-6815
2190-6823
ST - Bacterial cell-free metabolites-based zinc oxide nanoparticles for combating
skin-causing bacterial infections
T2 - BIOMASS CONVERSION AND BIOREFINERY
TI - Bacterial cell-free metabolites-based zinc oxide nanoparticles for combating
skin-causing bacterial infections
ID - 6755
ER -
TY - JOUR
AB - Fragaria ananassa, also known as "Strawberry" is a common species in Iran and
widely used for its anti-inflammatory, anti-ulcer, astringent, anti-allergic,
antibacterial, antifungal, and antidiarrheal activities and also in the treatment
of skin wounds. The purpose of the study was chemical characterization and
assessment of cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous
wound healing properties of copper nanoparticles (CuNPs) using the aqueous extract
of Strawberry fruit and L-Ascorbic acid as reducing and stabilizing agents. These
nanoparticles were characterized by FT-IR, UV-visible spectroscopy, EDS, FE-SEM,
and TEM analysis. TEM images exhibited a uniform spherical morphology and diameters
of 10-30 nm for the biosynthesized nanoparticles. DPPH free radical scavenging test
revealed similar antioxidant properties for Strawberry, CuNPs, and butylated
hydroxytoluene. The Strawberry and synthesized CuNPs had great cell viability dose-
dependently against HUVEC cell line. In the microbiological part of this study,
CuNPs showed higher antibacterial and antifungal properties than all standard
antibiotics (p <= 0.01). Also, CuNPs prevented the growth of all bacteria at 2-8
mg/mL concentrations and destroyed them at 2-16 mg/mL concentrations (p <= 0.01).
In the case of antifungal property of CuNPs, they inhibited the growth of all fungi
at 2-4 mg/mL concentrations and destroyed them at 2-8 mg/mL concentrations (p <=
0.01). In vivo design, the use of CuNPs ointment in the treatment groups
substantially remarkably raised (p <= 0.01) the wound contracture, hydroxyl
proline, hexosamine, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and
reduced (p <= 0.01) the wound area, total cells, neutrophil, macrophage, and
lymphocyte compared to Strawberry, CuSO4, tetracycline, Eucerin basal, and
untreated control groups. In conclusion, the results of chemical characterization
confirm that the Strawberry fruit can be consumed to produce copper nanoparticles
with a remarkable amount of remedial effects without any cytotoxicity against
HUVECs. (C) 2020 Elsevier Ltd. All rights reserved.
AN - WOS:000523765000028
AU - Hemmati, S.
AU - Ahmeda, A.
AU - Salehabadi, Y.
AU - Zangeneh, A.
C7 - 114425
DA - APR 1
DO - 10.1016/j.poly.2020.114425
PY - 2020
SN - 0277-5387
1873-3719
ST - Synthesis, characterization, and evaluation of cytotoxicity, antioxidant,
antifungal, antibacterial, and cutaneous wound healing effects of copper
nanoparticles using the aqueous extract of Strawberry fruit and L-Ascorbic acid
T2 - POLYHEDRON
TI - Synthesis, characterization, and evaluation of cytotoxicity, antioxidant,
antifungal, antibacterial, and cutaneous wound healing effects of copper
nanoparticles using the aqueous extract of Strawberry fruit and L-Ascorbic acid
VL - 180
ID - 6381
ER -
TY - JOUR
AB - The recent study reveals the cytotoxicity, antioxidant, and anti-acute
myeloid leukemia properties of polydopamine (PDA)-capped silver nanoparticles
(AgNPs) compared to doxorubicin in a leukemic mouse model. The synthesized AgNPs
were characterized using different techniques including ultraviolet–visible
spectroscopy (UV–Vis.), Fourier-transform infrared spectroscopy (FT-IR)
spectroscopy, X-ray diffraction (XRD), energy Dispersive X-ray Spectrometry (EDS),
field emission-scanning electron microscopy (FE-SEM), and transmission electron
microscopy (TEM). FE-SEM and TEM images revealed a uniform spherical morphology and
average diameters of 15–25 nm for the nanoparticles. Also, in XRD analysis, ~20 nm
was measured for the crystal size of nanoparticles. The results of the biological
experiments were fed into SPSS-22 software and analyzed by one-way ANOVA. In the
biological in vitro part of this study, AgNPs similar to doxorubicin had low cell
viability dose-dependently against Human HL-60/vcr, 32D-FLT3-ITD, and Murine C1498
cell lines without any cytotoxicity on HUVEC cell line. In this study, 2,2-
diphenyl-1-picrylhydrazyl (DPPH) test revealed similar antioxidant potentials for
doxorubicin and AgNPs. In the biological in vivo part of this study, induction of
acute myeloid leukemia was done by 7,12-Dimethylbenz[a]anthracene (DMBA) in 75
mice. Then, the animals were randomly divided into six subgroups, including
control, untreated, doxorubicin, AgNPs, PDA, and AgNO3. AgNPs similar to
doxorubicin, significantly (p ≤ 0.05) reduced the pro-inflammatory cytokines, and
the total white blood cell (WBC), blast, monocyte, neutrophil, eosinophil, and
basophil counts and enhanced the anti-inflammatory cytokines and the platelet,
lymphocyte, and red blood cell (RBC) parameters as compared to the untreated mice.
By quantitative real-time polymerase chain reaction (PCR), sphingosine-1-phosphate
receptor-1 (S1PR1) and sphingosine-1-phosphate receptor-5 (S1PR5) mRNA expression
in lymphocytes were significantly (p ≤ 0.05) raised by treating the leukemic mice
with the AgNPs and doxorubicin. Above results confirm the therapeutic effects of
AgNPs on acute myeloid leukemia in the leukemic mice. Further clinical trials are
necessary for confirmation these remedial properties of AgNPs in human. © 2019 John
Wiley & Sons, Ltd.
AU - Hemmati, S.
AU - Joshani, Z.
AU - Zangeneh, A.
C7 - e5277
DB - Scopus
DO - 10.1002/aoc.5277
IS - 2
doxorubicin
leukemic mouse model
polydopamine
Alkanolamines
Antioxidants
Biochemistry
Blood
Cell culture
Diseases
Enamels
Field emission microscopes
Lymphocytes
Mammals
Metal nanoparticles
Silver compounds
Sphingosines
X ray diffraction
Doxorubicin
Energy dispersive x-ray spectrometries (EDS)
Field emission scanning electron microscopy
Fourier transform infra red (FTIR) spectroscopy
Mouse models
Polydopamine
Quantitative real-time polymerase chain reaction
M3 - Article
PY - 2020
ST - Biosynthesis and chemical characterization of polydopamine-capped silver
nanoparticles for the treatment of acute myeloid leukemia in comparison to
doxorubicin in a leukemic mouse model
TI - Biosynthesis and chemical characterization of polydopamine-capped silver
nanoparticles for the treatment of acute myeloid leukemia in comparison to
doxorubicin in a leukemic mouse model
85076193172&doi=10.1002%2faoc.5277&partnerID=40&md5=584e5f6eca176d44de04fe8390fffde
9
VL - 34
ID - 5351
ER -
TY - JOUR
AB - Nanoparticles usually have better outcomes than the bulk samples of the same
element because they possess a higher specificity level than the larger particles.
This is also true for silver nanoparticles, and little amount of these materials
has high remedial effects. Silver nanoparticles are used as a therapeutic tool for
the treatment of several diseases such as cancer. In this study, silver
nanoparticles using chitosan (AgNPs-chitosan composite) are reported for the first
time to exert a dietary therapeutic potential compared to Daunorubicin in an animal
model of acute myeloid leukemia. The synthesized AgNPs-chitosan composite was
characterized using different techniques including ultraviolet–visible
spectroscopy, fourier-transform infrared spectroscopy, energy dispersive X-ray
spectrometry, scanning electron microscopy, and transmission electron microscopy.
FTIR findings suggested antioxidant compounds in the nanoparticles were the sources
of reducing power, reducing silver ions to AgNPs. SEM and TEM images exhibited a
uniform spherical morphology and average diameters of 30 nm for the nanoparticles.
Then, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging test was done to
evaluate the antioxidant potentials of Daunorubicin, AgNO3, chitosan, and AgNPs-
chitosan composite. DPPH test revealed similar antioxidant potentials for
Daunorubicin and AgNPs-chitosan composite. For the analyzing of cytotoxicity
effects of Daunorubicin, AgNO3, chitosan, and AgNPs, 3-(4,5-Dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromidefor (MTT) assay was used on HUVEC, 32D-FLT3-ITD, and
Murine C1498 cell lines. AgNPs-chitosan composite similar to Daunorubicin had low
cell viability dose-dependently against 32D-FLT3-ITD and Murine C1498 cell lines
without any cytotoxicity on HUVEC cell line. In vivo design, induction of acute
myeloid leukemia was done by 7,12-Dimethylbenz[a]anthracene in 50 mice. Then, the
animals were randomly divided into six subgroups, including control, untreated,
Daunorubicin, AgNO3, chitosan, and AgNPs-chitosan composite. Similar to
Daunorubicin, AgNPs-chitosan composite significantly (P ≤.01) decreased the weight
of the body, the pro-inflammatory cytokines, and the total white blood cells,
blast, neutrophil, monocyte, eosinophil, and basophil counts and increased the
anti-inflammatory cytokines and the lymphocyte, platelet, and red blood cell
parameters as compared to the untreated mice. These results show that the inclusion
of chitosan improves the therapeutic properties of AgNPs-chitosan composite, which
led to a significant enhancement in the antioxidant, cytotoxicity, and anti-acute
myeloid leukemia activities of the nanoparticles. It appears that AgNPs-chitosan
composite can be used as a chemotherapeutic drug for the treatment of acute myeloid
leukemia in the clinical trial. © 2019 John Wiley & Sons, Ltd.
AU - Hemmati, S.
AU - Zamenian, T.
AU - Delsooz, N.
AU - Zangeneh, A.
AU - Mahdi Zangeneh, M.
C7 - e5274
DB - Scopus
DO - 10.1002/aoc.5274
IS - 2
chitosan
Daunorubicin
Antioxidants
Blood
Cell culture
Cells
Chemical analysis
Chitosan
Cytotoxicity
Diseases
Free radicals
Mammals
Metal ions
Metal nanoparticles
Silver compounds
1 ,1-diphenyl-2-picrylhydrazyl
Energy dispersive X-ray spectrometry
Free radical scavenging
Spherical morphologies
Drug delivery
M3 - Article
PY - 2020
ST - Preparation and synthesis a new chemotherapeutic drug of silver nanoparticle-
chitosan composite; Chemical characterization and analysis of their antioxidant,
cytotoxicity, and anti-acute myeloid leukemia effects in comparison to Daunorubicin
in a leukemic mouse model
TI - Preparation and synthesis a new chemotherapeutic drug of silver nanoparticle-
85076380141&doi=10.1002%2faoc.5274&partnerID=40&md5=5d57ab20effb80be3e5d79c28007754
d
VL - 34
ID - 5338
ER -
TY - JOUR
AB - Nanoparticles usually have better outcomes than the bulk samples of the same
element because they possess a higher specificity level than the larger particles.
This is also true for silver nanoparticles, and little amount of these materials
has high remedial effects. Silver nanoparticles are used as a therapeutic tool for
the treatment of several diseases such as cancer. In this study, silver
nanoparticles using chitosan (AgNPs-chitosan composite) are reported for the first
time to exert a dietary therapeutic potential compared to Daunorubicin in an animal
model of acute myeloid leukemia. The synthesized AgNPs-chitosan composite was
characterized using different techniques including ultraviolet-visible
spectroscopy, fourier-transform infrared spectroscopy, energy dispersive X-ray
spectrometry, scanning electron microscopy, and transmission electron microscopy.
FTIR findings suggested antioxidant compounds in the nanoparticles were the sources
of reducing power, reducing silver ions to AgNPs. SEM and TEM images exhibited a
uniform spherical morphology and average diameters of 30 nm for the nanoparticles.
Then, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging test was done to
evaluate the antioxidant potentials of Daunorubicin, AgNO3, chitosan, and AgNPs-
chitosan composite. DPPH test revealed similar antioxidant potentials for
Daunorubicin and AgNPs-chitosan composite. For the analyzing of cytotoxicity
effects of Daunorubicin, AgNO3, chitosan, and AgNPs, 3-(4,5-Dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromidefor (MTT) assay was used on HUVEC, 32D-FLT3-ITD, and
Murine C1498 cell lines. AgNPs-chitosan composite similar to Daunorubicin had low
cell viability dose-dependently against 32D-FLT3-ITD and Murine C1498 cell lines
without any cytotoxicity on HUVEC cell line. In vivo design, induction of acute
myeloid leukemia was done by 7,12-Dimethylbenz[a]anthracene in 50 mice. Then, the
animals were randomly divided into six subgroups, including control, untreated,
Daunorubicin, AgNO3, chitosan, and AgNPs-chitosan composite. Similar to
Daunorubicin, AgNPs-chitosan composite significantly (P <= .01) decreased the
weight of the body, the pro-inflammatory cytokines, and the total white blood
cells, blast, neutrophil, monocyte, eosinophil, and basophil counts and increased
the anti-inflammatory cytokines and the lymphocyte, platelet, and red blood cell
parameters as compared to the untreated mice. These results show that the inclusion
of chitosan improves the therapeutic properties of AgNPs-chitosan composite, which
led to a significant enhancement in the antioxidant, cytotoxicity, and anti-acute
myeloid leukemia activities of the nanoparticles. It appears that AgNPs-chitosan
composite can be used as a chemotherapeutic drug for the treatment of acute myeloid
leukemia in the clinical trial.
AN - WOS:000501526400001
AU - Hemmati, S.
AU - Zamenian, T.
AU - Delsooz, N.
AU - Zangeneh, A.
C6 - DEC 2019
C7 - e5274
DA - FEB
DO - 10.1002/aoc.5274
IS - 2
PY - 2020
SN - 0268-2605
1099-0739
ST - Preparation and synthesis a new chemotherapeutic drug of silver nanoparticle-
T2 - APPLIED ORGANOMETALLIC CHEMISTRY
TI - Preparation and synthesis a new chemotherapeutic drug of silver nanoparticle-
VL - 34
ID - 6049
ER -
TY - JOUR
AB - Phosphorylase kinase (PhK) is a unique enzyme in which the spatial
arrangements of the specificity determinants can be manipulated to allow the enzyme
to recognize substrates of different specificities. In this way, PhK is capable of
transferring high energy phosphate bonds from ATP to serine/threonine and tyrosine
moieties in serine/threonine kinases and tyrosine kinases, thus playing a key role
in the activation of multiple signaling pathways. Phosphorylase kinase is released
within five minutes following injury and is responsible for activating inflammatory
pathways in injury-activated scarring following burns. In photo-damaged skin, PhK
plays an important role in promoting photocarcinogenesis through activation of NF-
kB-dependent signaling pathways with inhibition of apoptosis of photo-damaged
cells, thus promoting the survival of precancerous cells and allowing for
subsequent tumor transformation. Curcumin, the active ingredient in the spice,
turmeric, is a selective and non-competitive PhK inhibitor. By inhibition of PhK,
curcumin targets multiple PhK-dependent pathways, with salutary effects on a number
of skin diseases induced by injury. In this paper, we show that curcumin gel
produces rapid healing of burns, with little or no residual scarring. Curcumin gel
is also beneficial in the repair of photo-damaged skin, including pigmentary
changes, solar elastosis, thinning of the skin with telangiectasia (actinic
poikiloderma), and premalignant lesions such as actinic keratoses, dysplastic nevi,
and advanced solar lentigines, but the repair process takes many months. © 2012 The
International Society of Dermatology.
AU - Heng, M. C. Y.
DB - Scopus
DO - 10.1111/j.1365-4632.2012.05703.x
IS - 5
KW - Apoptosis
Burns
Curcumin
Enzyme Inhibitors
Gels
Humans
I-kappa B Kinase
NF-kappa B
Phosphorylase Kinase
Signal Transduction
Skin Aging
Skin Diseases
alitretinoin
curcumin
cyclin dependent kinase inhibitor 1
I kappa B kinase alpha
phosphorylase kinase
prednisone
protein kinase B
protein p53
pyrimidine dimer
retinoid
sulfadiazine silver
actinic keratosis
apoptosis
burn
cell proliferation
cell survival
DNA replication
double stranded DNA break
dysplastic nevus
human
lentigo
malignant transformation
photoaging
phototoxicity
point mutation
precancer
protein expression
review
signal transduction
skin carcinogenesis
skin injury
sunburn
M3 - Review
PY - 2013
SP - 531-543
ST - Signaling pathways targeted by curcumin in acute and chronic injury: Burns
and photo-damaged skin
T2 - International Journal of Dermatology
TI - Signaling pathways targeted by curcumin in acute and chronic injury: Burns
and photo-damaged skin
84876452159&doi=10.1111%2fj.1365-
4632.2012.05703.x&partnerID=40&md5=d38c3195df9cf5d20653230f0c8baae9
VL - 52
ID - 5754
ER -
TY - JOUR
AB - Purpose: Propolis, a natural honey bee hive product, has anti-inflammatory
and antioxidative properties. We aimed to assess the possible contribution of
topically applied propolis to the suppression of corneal neovascularization (CNV).
Methods: The effect of a water extract of propolis (WEP) 1% drops (group 1) in
comparison with dexamethasone 0.1% (group 2) and saline (group 3) on CNV was tested
in rabbit corneas injured by silver nitrate cauterization. The extent of CNV was
quantitated as the area of CNV and the percent area of CNV for each cornea of the
three groups (12 right eyes per group) in the first week of the treatment. The mean
percent CNV was used for statistical analysis. Results: The corneas treated with
the topical WEP 1% had an almost equal percent CNV as compared with the corneas
treated with topical dexamethasone 0.1% and had less percent CNV than the control
eyes. The quantitative analysis in groups 1, 2 and 3 revealed that the mean percent
CNV was 41.0 +/- 14.1, 39.4 +/- 11.0 and 56.9 +/- 18.4, respectively. The
differences between both groups 1 and 3 as well as groups 2 and 3 were
statistically significant (p = 0.02 and p = 0.01, respectively), whereas the
difference between groups 1 and 2 was not significant (p = 0.86). Conclusions The
topical application of a WEP 1% has an inhibitory effect on CNV in the rabbit's
cornea. The inhibitory effect of propolis was shown to be comparable to that of
topical dexamethasone 0.1%, a potent inhibitor of angiogenesis. We suggest that the
effect of propolis may partially be due to its inhibitory effect on the activity of
both cyclo-oxygenase and lipo-oxygenase.
AN - WOS:000082988400006
AU - Hepsen, I. F.
AU - Er, H.
AU - Cekic, O.
DA - NOV-DEC
DO - 10.1159/000055567
IS - 6
PY - 1999
SN - 0030-3747
SP - 426-431
ST - Topically applied water extract of propolis to suppress corneal
neovascularization in rabbits
T2 - OPHTHALMIC RESEARCH
TI - Topically applied water extract of propolis to suppress corneal
neovascularization in rabbits
VL - 31
ID - 6778
ER -
TY - JOUR
AB - Background: Due to its antibacterial properties, silver (Ag) has been used in
more consumer products than any other nanomaterial so far. Despite the promising
advantages posed by using Ag-nanoparticles (NPs), their interaction with mammalian
systems is currently not fully understood. An exposure route via inhalation is of
primary concern for humans in an occupational setting. Aim of this study was
therefore to investigate the potential adverse effects of aerosolised Ag-NPs using
a human epithelial airway barrier model composed of A549, monocyte derived
macrophage and dendritic cells cultured in vitro at the air-liquid interface. Cell
cultures were exposed to 20 nm citrate-coated Ag-NPs with a deposition of 30 and
278 ng/cm2 respectively and incubated for 4 h and 24 h. To elucidate whether any
effects of Ag-NPs are due to ionic effects, Ag-Nitrate (AgNO3) solutions were
aerosolised at the same molecular mass concentrations.Results: Agglomerates of Ag-
NPs were detected at 24 h post exposure in vesicular structures inside cells but
the cellular integrity was not impaired upon Ag-NP exposures. Minimal cytotoxicity,
by measuring the release of lactate dehydrogenase, could only be detected following
a higher concentrated AgNO3-solution. A release of pro-inflammatory markers TNF-α
and IL-8 was neither observed upon Ag-NP and AgNO3 exposures as well as was not
affected when cells were pre-stimulated with lipopolysaccharide (LPS). Also, an
induction of mRNA expression of TNF-α and IL-8, could only be observed for the
highest AgNO3 concentration alone or even significantly increased when pre-
stimulated with LPS after 4 h. However, this effect disappeared after 24 h.
Furthermore, oxidative stress markers (HMOX-1, SOD-1) were expressed after 4 h in a
concentration dependent manner following AgNO3 exposures only.Conclusions: With an
experimental setup reflecting physiological exposure conditions in the human lung
more realistic, the present study indicates that Ag-NPs do not cause adverse
effects and cells were only sensitive to high Ag-ion concentrations. Chronic
exposure scenarios however, are needed to reveal further insight into the fate of
Ag-NPs after deposition and cell interactions. © 2013 Herzog et al.; licensee
BioMed Central Ltd.
AU - Herzog, F.
AU - Clift, M. J. D.
AU - Piccapietra, F.
AU - Behra, R.
AU - Schmid, O.
AU - Petri-Fink, A.
AU - Rothen-Rutishauser, B.
C7 - 11
DB - Scopus
DO - 10.1186/1743-8977-10-11
IS - 1
KW - Aerosols
Biological Markers
Blood-Air Barrier
Cell Line, Tumor
Cytokines
Heme Oxygenase-1
Humans
Inhalation Exposure
Lipopolysaccharides
Metal Nanoparticles
Oxidative Stress
Risk Assessment
RNA, Messenger
Silver Nitrate
Time Factors
Mammalia
copper zinc superoxide dismutase
heme oxygenase 1
interleukin 8
lipopolysaccharide
silver
silver nanoparticle
silver nitrate
aerosol
autacoid
biological marker
cytokine
HMOX1 protein, human
messenger RNA
metal nanoparticle
air liquid interface cell exposure
article
controlled study
cytokine release
cytotoxicity
gene expression
HMOX 1 gene
human
human cell
IL 8 gene
in vitro study
inflammation
lung alveolus cell
nucleotide sequence
oxidative stress
priority journal
procedures concerning cells
SOD 1 gene
TNF alpha gene
toxicity testing
adverse effects
coculture
dose response
drug effects
exposure
genetics
lung gas exchange
metabolism
risk assessment
time
tumor cell line
M3 - Article
PY - 2013
ST - Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at
the air-liquid interface
TI - Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at
the air-liquid interface
84875690495&doi=10.1186%2f1743-8977-10-
11&partnerID=40&md5=a54ad3947ca3c5e5696e126a12e319c4
VL - 10
ID - 5724
ER -
TY - JOUR
AB - In the emerging market of nano-sized products, silver nanoparticles (Ag NPs)
are widely used due to their antimicrobial properties. Human interaction with Ag
NPs can occur through the lung, skin, gastrointestinal tract, and bloodstream.
However, the inhalation of Ag NP aerosols is a primary concern. To study the
possible effects of inhaled Ag NPs, an in vitro triple cell co-culture model of the
human alveolar/airway barrier (A549 epithelial cells, human peripheral blood
monocyte derived dendritic and macrophage cells) together with an air-liquid
interface cell exposure (ALICE) system was used in order to reflect a real-life
exposure scenario. Cells were exposed at the air-liquid interface (ALI) to 0.03,
0.3, and 3 μg Ag/cm2 of Ag NPs (diameter 100 nm; coated with polyvinylpyrrolidone:
PVP). Ag NPs were found to be highly aggregated within ALI exposed cells with no
impairment of cell morphology. Furthermore, a significant increase in release of
cytotoxic (LDH), oxidative stress (SOD-1, HMOX-1) or pro-inflammatory markers (TNF-
α, IL-8) was absent. As a comparison, cells were exposed to Ag NPs in submerged
conditions to 10, 20, and 30 μg Ag/mL. The deposited dose per surface area was
estimated by using a dosimetry model (ISDD) to directly compare submerged vs ALI
exposure concentrations after 4 and 24 h. Unlike ALI exposures, the two highest
concentrations under submerged conditions promoted a cytotoxic and pro-inflammatory
response after 24 h. Interestingly, when cell cultures were co-incubated with
lipopolysaccharide (LPS), no synergistic inflammatory effects were observed. By
using two different exposure scenarios it has been shown that the ALI as well as
the suspension conditions for the lower concentrations after 4 h, reflecting
reallife concentrations of an acute 24 h exposure, did not induce any adverse
effects in a complex 3D model mimicking the human alveolar/airway barrier. However,
the highest concentrations used in the ALI setup, as well as all concentrations
under submerged conditions after 24 h, reflecting more of a chronic lifetime
exposure concentration, showed cytotoxic as well as pro-inflammatory effects. In
conclusion, more studies need to address long-term and chronic Ag NP exposure
effects. © 2014 Herzog et al.
AU - Herzog, F.
AU - Loza, K.
AU - Balog, S.
AU - Epple, M.
AU - Gehr, P.
AU - Petri-Fink, A.
DB - Scopus
DO - 10.3762/bjnano.5.149
IS - 1
KW - Air-liquid exposure
Dosimetry
Lung cells in vitro
Toxicity
Biological organs
Cell culture
Liquids
Metal nanoparticles
Phase interfaces
Plants (botany)
Air liquid interfaces
Anti-microbial properties
Exposure concentration
Human peripheral blood
In-vitro
Poly vinyl pyrrolidone
Cells
M3 - Article
PY - 2014
SP - 1357-1370
ST - Mimicking exposures to acute and lifetime concentrations of inhaled silver
nanoparticles by two different in vitro approaches
T2 - Beilstein Journal of Nanotechnology
TI - Mimicking exposures to acute and lifetime concentrations of inhaled silver
nanoparticles by two different in vitro approaches
84987677215&doi=10.3762%2fbjnano.5.149&partnerID=40&md5=bc253756dd8e1d264f1033bdd87
c21ca
VL - 5
ID - 5558
ER -
TY - JOUR
AB - Background Peroxisome proliferator activated receptor alpha (PPARα), a
regulator of enzymes involved in β oxidation, has been reported to influence
lymphocyte activation. The purpose of this study was to determine whether PPARα
plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA). Methods
Wild type (wt) or PPARα-deficient (PPARα−/−) mice were treated with ConA (15 mg/kg)
by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue
collection for analysis of tissue injury, cytokine response, T cell activation and
characterization. Results Ten and 24 h following ConA administration, wt mice had
significant liver injury as demonstrated by serum transaminase levels, inflammatory
cell infiltrate, hepatocyte apoptosis, and expression of several cytokines
including interleukin 4 (IL4) and interferon gamma (IFNγ). In contrast, PPARα−/−
mice were protected from ConA-induced liver injury with significant reductions in
serum enzyme release, greatly reduced inflammatory cell infiltrate, hepatocellular
apoptosis, and IFNγ expression, despite having similar levels of hepatic T cell
activation and IL4 expression. This resistance to liver injury was correlated with
reduced numbers of hepatic natural killer T (NKT) cells and their in vivo
responsiveness to alpha-galactosylceramide. Interestingly, adoptive transfer of
either wt or PPARα−/− splenocytes reconstituted ConA liver injury and cytokine
production in lymphocyte-deficient, severe combined immunodeficient mice
implicating PPARα within the liver, possibly through support of IL15 expression
and/or suppression of IL12 production and not the lymphocyte as the key regulator
of T cell activity and ConA-induced liver injury. Conclusion Taken together, these
data suggest that PPARα within the liver plays an important role in ConA-mediated
liver injury through regulation of NKT cell recruitment and/or survival.
AD - Hines, Ian N.
East Carolina University. College of Allied Health Sciences. Department of
Nutrition Science. Greenville. US
Kremer, Michael
Moore, Sherri M.
Wheeler, Michael D.
AU - Hines, Ian N.
AU - Kremer, Michael
AU - Moore, Sherri M.
AU - Wheeler, Michael D.
C1 - 20180309
DA - 2018/00
DB - LILACS
DO - 10.1186/s40659-018-0153-z
KW - Cytokines
Inflammation
Interferon gamma
T helper phenotype
LA - en
PY - 2018
SN - 0716-9760
SP - 5-5
ST - Impaired T cell-mediated hepatitis in peroxisome proliferator activated
receptor alpha (PPARα)-deficient mice
T2 - Biol. Res
TI - Impaired T cell-mediated hepatitis in peroxisome proliferator activated
receptor alpha (PPARα)-deficient mice
97602018000100204
VL - 51
ID - 4926
ER -
TY - JOUR
AB - Precision-cut lung slices (PCLS) are an established ex vivo alternative to in
vivo experiments in pharmacotoxicology. The aim of this study was to evaluate the
potential of PCLS as a tool in nanotoxicology studies. Silver (Ag-NPs) and zinc
oxide (ZnO-NPs) nanoparticles as well as quartz particles were used because these
materials have been previously shown in several in vitro and in vivo studies to
induce a dose-dependent cytotoxic and inflammatory response. PCLS were exposed to
three concentrations of 70 nm monodisperse polyvinylpyrrolidone (PVP)-coated Ag-NPs
under submerged culture conditions in vitro. ZnO-NPs (NM110) served as 'soluble'
and quartz particles (Min-U-Sil) as 'non-soluble' control particles. After 4 and 24
h, the cell viability and the release of proinflammatory cytokines was measured. In
addition, multiphoton microscopy was employed to assess the localization of Ag-NPs
in PCLS after 24 h of incubation. Exposure of PCLS to ZnO-NPs for 4 and 24 h
resulted in a strong decrease in cell viability, while quartz particles had no
cytotoxic effect. Moreover, only a slight cytotoxic response was detected by LDH
release after incubation of PCLS with 20 or 30 μg/mL of Ag-NPs. Interestingly, none
of the particles tested induced a proinflammatory response in PCLS. Finally,
multiphoton microscopy revealed that the Ag-NP were predominantly localized at the
cut surface and only to a much lower extent in the deeper layers of the PCLS. In
summary, only 'soluble' ZnO-NPs elicited a strong cytotoxic response. Therefore, we
suggest that the cytotoxic response in PCLS was caused by released Zn2+ ions rather
than by the ZnO-NPs themselves. Moreover, Ag-NPs were predominantly localized at
the cut surface of PCLS but not in deeper regions, indicating that the majority of
the particles did not have the chance to interact with all cells present in the
tissue slice. In conclusion, our findings suggest that PCLS may have some
limitations when used for nanotoxicology studies. To strengthen this conclusion,
however, other NP types and concentrations need to be tested in further studies. ©
2014 Hirn et al.
AU - Hirn, S.
AU - Haberl, N.
AU - Loza, K.
AU - Epple, M.
AU - Rehberg, M.
AU - Krombach, F.
DB - Scopus
DO - 10.3762/bjnano.5.253
IS - 1
KW - Cytokines
Cytotoxicity
Ex vivo
Lung slices
Nanoparticles
Biological organs
Quartz
Zinc oxide
Ex-vivo
Multi-photon microscopy
Proinflammatory response
Submerged culture condition
M3 - Article
PY - 2014
SP - 2440-2449
ST - Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung
slices
T2 - Beilstein Journal of Nanotechnology
TI - Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung
slices
84987625580&doi=10.3762%2fbjnano.5.253&partnerID=40&md5=cccddf9935efbd73bbe64287d80
b385d
VL - 5
ID - 5565
ER -
TY - JOUR
AB - Background Torsion of the spermatic cord and the resulting testicular
ischemia leads to the production of in-flammatory cytokines and cell death due to
impaired aerobic metabolism. Following reperfusion of the testis, a robust innate
inflammatory response fur-thers tissue injury due to the production of reactive
oxygen species and disruption of normal capillary function. Blunting the innate
immune response with antioxidants, anti-inflammatory medications and targeted
genetic interventions reduces long term testicular injury in animal models of
torsion, how-ever these approaches have limited clinical appli-cability. Mediated
via a7 nACh receptors, the cholinergic anti-inflammatory pathway limits NFKB
signaling and prevents renal fibrosis following warm renal ischemia. We identified
varenicline as an FDA approved a7 nAChR agonist and hypothesized that varenicline
administration would decrease long-term testicular atrophy and fibrosis in a murine
model of testicular torsion. Methods Using an established model, unilateral
testicular torsion was induced in mature male CD1 mice by rotating the right
testicle 720 degrees for 2 h. In the treatment group, 4 doses of varenicline
(1mg/grm)were administered via intraperitoneal injection every 12 h, with the first
dose given 1 h after the creation of testicular torsion. The acute inflamma-tory
response was evaluated 48 h following reper-fusion of the testis. Long term
outcomes were evaluated 30 days following testicular perfusion. Results 48 h
following reperfusion, the testis of animals treated with varenicline demonstrated
a significant reduction in the inflammatory response as measured by the acute
immune cell infiltrate, myeloperox-idase activity, concentration of reduced
glutathione and expression of downstream NF-KB targets. 30 days following
reperfusion, animals treated with varenicline, demonstrated decreased testicular
at-rophy (Summary Figure), fibrosis and expression of pro-fibrotic genes.
Conclusion Activation of a central immunosuppressive cascade with varenicline after
the onset of testicular torsion reduces ischemia reperfusion injury and prevents
long term testicular atrophy and fibrosis. Further studies are needed to define the
optimum dose and varenicline administration regimen. Our results suggest that
varenicline offers a novel, FDA approved, adjunct to the current management of
testicular torsion.
AN - WOS:000714954600007
AU - Ho, C.
AU - Zee, R. S.
AU - Omidi, N.
AU - Bayne, C.
AU - Hester, A.
AU - Mukherjee, E.
AU - Sims-Lucas, S.
AU - Mbanefo, E. C.
AU - Hsieh, M. H.
AU - Casella, D. P.
C6 - NOV 2021
DA - OCT
DO - 10.1016/j.jpurol.2021.07.004
IS - 5
PY - 2021
SN - 1477-5131
1873-4898
ST - Varenicline limits ischemia reperfusion injury following testicular torsion
in mice
T2 - JOURNAL OF PEDIATRIC UROLOGY
TI - Varenicline limits ischemia reperfusion injury following testicular torsion
in mice
VL - 17
ID - 6639
ER -
TY - JOUR
AB - Gold (Au) is long considered as a biocompatible metal, and gold nanoparticles
(AuNPs ∼5 nm) were recently reported to scavenge free radicals. The effect of Au
embedded in a polymeric material is less investigated compared with that of silver.
In this study, nanocomposites from polyurethane (PU) and 43.5 or 174 ppm of AuNPs
were prepared from a waterborne process. The response of endothelial cells (ECs) to
the PU-Au nanocomposites was investigated in vitro and in vivo. ECs on PU-Au
nanocomposites showed lamellipodia formation and better cell proliferation. The
activation of proteins in ECs grown on PU-Au nanocomposites was analyzed by two-
dimensional gel electrophoresis and confirmed by Western blot. The new protein
identified through this procedure was valosin-containing protein (VCP), which is
known to have immunomodulating effect. VCP was upregulated by PU-Au 43.5 ppm and
PU-Au 174 ppm, but more in PU-Au 43.5 ppm. This suggested that the dispersion of
AuNPs in the polymer matrix may be more important than the loading amount. PU-Au
catheters implanted in rat blood vessels showed less foreign body reaction and more
extensive EC coverage than the control PU catheters. The good in vivo
biocompatibility of PU-Au may be associated with the antiinflammatory effect of PU-
Au. Based on this study, AuNPs may serve as an antioxidant additive for biomedical
polymers. © The Author(s) 2012. This article is published with open access at
Springerlink.com.
AU - Ho, T. T.
AU - Lin, Y. C.
AU - Hsu, S. H.
DB - Scopus
DO - 10.1007/s13404-012-0062-9
IS - 3
Gold nanoparticles (AuNPs)
Polymer nanocomposites
Two-dimensional gel electrophoresis
Additives
Biocompatibility
Blood vessels
Catheters
Cell proliferation
Electrophoresis
Endothelial cells
Free radicals
Functional polymers
Gold
Loading
Metal nanoparticles
Polyurethanes
Proteins
Rat control
Two dimensional
Anti-inflammatories
Antioxidant additives
Biocompatible metals
Biomedical polymers
Gold nanoparticle
Gold Nanoparticles
In-vitro
In-vivo
Lamellipodia
Loading amount
Polymer nanocomposite
Western blots
Nanocomposites
M3 - Article
PY - 2012
SP - 161-170
ST - Human endothelial cell response to polyurethane-gold nanocomposites
T2 - Gold Bulletin
TI - Human endothelial cell response to polyurethane-gold nanocomposites
84868155542&doi=10.1007%2fs13404-012-0062-
9&partnerID=40&md5=fb245b5f682a55a124f9fe1263f32360
VL - 45
ID - 5660
ER -
TY - JOUR
AB - The inhalation or application of nanoparticles (NPs) has serious impacts on
immunological reactivity. However, the effects of NPs on the immune system are
influenced by numerous factors, which cause a high variability in the results.
Here, mice were exposed to a three month continuous inhalation of copper oxide
(CuO) NPs, and at different time intervals (3, 14, 42 and 93 days), the composition
of cell populations of innate and adaptive immunity was evaluated in the spleen by
flow cytometry. The ability of spleen cells from exposed and control mice to
respond to stimulation with T- or B-cell mitogens by proliferation and by
production of cytokines IL-2, IL-6, IL-10, IL-17 and IFN-gamma was characterized.
The results showed that the inhalation of CuO NPs predominantly affects the cells
of innate immunity (changes in the proportion of eosinophils, neutrophils,
macrophages and antigen-presenting cells) with a minimal effect on the percentage
of T and B lymphocytes. However, the proliferative and secretory activity of T
cells was already significantly enhanced after 3 days from the start of inhalation,
decreased on day 14 and normalized at the later time intervals. There was no
correlation between the impacts of NPs on the cells of innate and adaptive
immunity. The results have shown that the inhalation of CuO NPs significantly
alters the composition of cell populations of innate immunity and modulates the
proliferation and production of cytokines by cells of the adaptive immune system.
However, the immunomodulatory effects of inhaled NPs strongly depend on the time of
inhalation.
AN - WOS:000469701900001
AU - Holan, V.
AU - Javorkova, E.
AU - Vrbova, K.
AU - Vecera, Z.
AU - Mikuska, P.
AU - Coufalik, P.
AU - Kulich, P.
AU - Skoupy, R.
AU - Machala, M.
AU - Zajicova, A.
AU - Rossner, P.
C6 - APR 2019
DA - AUG 9
DO - 10.1080/17435390.2019.1602679
IS - 7
PY - 2019
SN - 1743-5390
1743-5404
SP - 952-963
ST - A murine model of the effects of inhaled CuO nanoparticles on cells of innate
and adaptive immunity - a kinetic study of a continuous three-month exposure
T2 - NANOTOXICOLOGY
TI - A murine model of the effects of inhaled CuO nanoparticles on cells of innate
and adaptive immunity - a kinetic study of a continuous three-month exposure
VL - 13
ID - 6814
ER -
TY - JOUR
AB - This review highlights and discusses the impact of nanotechnology on the
inhibition of microbial colonization and biofilm development on modified surface
prosthetic devices. In the first part of the paper the current status of infections
related to prosthetic devices and the inquiries resulting from the increased number
of patients with these infections are briefly reviewed. Next we discuss several
aspects about the implication of nanotechnology in prosthetic devices surface
modification and its impact on the prevention of infections. The main aspects
regarding the biocompatibility and the application of these nanomodified prosthetic
devices in tissue engineering are also highlighted. © 2015 Bentham Science
Publishers
AU - Holban, A. M.
AU - Iordanskii, A.
AU - Bychkova, A.
AU - Andronescu, E.
AU - Mogoantă, L. I.
AU - Mogo Ș Anu, G. D.
AU - Iordache, F.
DB - Scopus
DO - 10.2174/138920101602150112150303
IS - 2
KW - Antimicrobial coating
Biocompatible nanoparticles
Biofilm inhibition
Modified surface
Prosthetic device
Animals
Bacterial Infections
Biofilms
Humans
Mycoses
Nanostructures
Nanotechnology
Prostheses and Implants
Surface Properties
alginic acid
alloy
aluminum oxide
cefotaxime
cellulose
chitin
collagen
erythromycin
hydroxyapatite
interleukin 12
kanamycin
metal oxide
molecular scaffold
nanomaterial
poly (methyl methacrylate)
polycaprolactone
polyglactin
polylactide
polymyxin B
polypropylene
polyvinyl alcohol
polyvinylchloride
silver nanoparticle
streptomycin
titanium dioxide
unclassified drug
zirconium oxide
biomaterial
Article
bacterial growth
bacterium contamination
biocompatibility
biodegradable implant
biofilm
bone remodeling
cell proliferation
ceramic prosthesis
clinical trial (topic)
growth inhibition
human
infection prevention
inflammatory infiltrate
material coating
nanotechnology
nonhuman
prosthesis infection
surface property
tissue engineering
tissue regeneration
animal
prostheses and orthoses
M3 - Article
PY - 2015
SP - 112-120
ST - Prosthetic devices with nanostructurated surfaces for increased resistance to
T2 - Current Pharmaceutical Biotechnology
TI - Prosthetic devices with nanostructurated surfaces for increased resistance to
84926151693&doi=10.2174%2f138920101602150112150303&partnerID=40&md5=22d3da349bcff86
0a938e14404f75301
VL - 16
ID - 5561
ER -
TY - JOUR
AB - Silver nanoparticles are one of the most prevalent nanomaterials in consumer
products. Some of these products are likely to be aerosolized, making silver
nanoparticles a high priority for inhalation toxicity assessment. To study the
inhalation toxicity of silver nanoparticles, we have exposed cultured lung cells to
them at the air-liquid interface. Cells were exposed to suspensions of silver or
nickel oxide (positive control) nanoparticles at concentrations of 2.6, 6.6, and
13.2 g cm-2 (volume concentrations of 10, 25, and 50 g ml-1) and to 0.7 μg cm-2
silver or 2.1 μg cm-2 nickel oxide aerosol at the air-liquid interface. Unlike a
number of in vitro studies employing suspensions of silver nanoparticles, which
have shown strong toxic effects, both suspensions and aerosolized nanoparticles
caused negligible cytotoxicity and only a mild inflammatory response, in agreement
with animal exposures. Additionally, we have developed a novel method using a
differential mobility analyzer to select aerosolized nanoparticles of a single
diameter to assess the size-dependent toxicity of silver nanoparticles. © 2013
Amara L. Holder and Linsey C. Marr.
AU - Holder, A. L.
AU - Marr, L. C.
C7 - 328934
DB - Scopus
DO - 10.1155/2013/328934
KW - Aerosols
Cell Line
Cytotoxins
Humans
Nanoparticles
Nickel
Oxides
Silver
Silver Compounds
silver nanoparticle
cytotoxin
disilver oxide
nanoparticle
nickel
nickel monoxide
oxide
silver
silver derivative
aerosol
air
article
controlled study
cytotoxicity
exposure
human
human cell
in vitro study
inflammation
liquid
lung
lung alveolus cell
particle size
prevalence
suspension
cell line
M3 - Article
PY - 2013
ST - Toxicity of silver nanoparticles at the air-liquid interface
T2 - BioMed Research International
TI - Toxicity of silver nanoparticles at the air-liquid interface
84874600868&doi=10.1155%2f2013%2f328934&partnerID=40&md5=d9cbd837534e6df2d1aabb0431
83fe5f
VL - 2013
ID - 5728
ER -
TY - JOUR
AB - Background: The uses of engineered nanomaterials have expanded in biomedical
technology and consumer manufacturing. Furthermore, pulmonary exposure to various
engineered nanomaterials has, likewise, demonstrated the ability to exacerbate
cardiac ischemia reperfusion (I/R) injury. However, the influence of particle size
or capping agent remains unclear. In an effort to address these influences we
explored response to 2 different size gold core nanosilver particles (AgNP) with
two different capping agents at 2 different time points. We hypothesized that a
pulmonary exposure to AgNP induces cardiovascular toxicity influenced by
inflammation and vascular dysfunction resulting in expansion of cardiac I/R Injury
that is sensitive to particle size and the capping agent. Methods: Male Sprague-
Dawley rats were exposed to 200 μg of 20 or 110 nm polyvinylprryolidone (PVP) or
citrate capped AgNP. One and 7 days following intratracheal instillation serum was
analyzed for concentrations of selected cytokines; cardiac I/R injury and isolated
coronary artery and aorta segment were assessed for constrictor responses and
endothelial dependent relaxation and endothelial independent nitric oxide dependent
relaxation. Results: AgNP instillation resulted in modest increase in selected
serum cytokines with elevations in IL-2, IL-18, and IL-6. Instillation resulted in
a derangement of vascular responses to constrictors serotonin or phenylephrine, as
well as endothelial dependent relaxations with acetylcholine or endothelial
independent relaxations by sodium nitroprusside in a capping and size dependent
manner. Exposure to both 20 and 110 nm AgNP resulted in exacerbation cardiac I/R
injury 1 day following IT instillation independent of capping agent with 20 nm AgNP
inducing marginally greater injury. Seven days following IT instillation the
expansion of I/R injury persisted but the greatest injury was associated with
exposure to 110 nm PVP capped AgNP resulted in nearly a two-fold larger infarct
size compared to na�ve. Conclusions: Exposure to AgNP may result in vascular
dysfunction, a potentially maladaptive sensitization of the immune system to
respond to a secondary insult (e.g., cardiac I/R) which may drive expansion of I/R
injury at 1 and 7 days following IT instillation where the extent of injury could
be correlated with capping agents and AgNP size. � 2016 The Author(s).
AU - Holland, N. A.
AU - Thompson, L. C.
AU - Vidanapathirana, A. K.
AU - Urankar, R. N.
AU - Lust, R. M.
AU - Fennell, T. R.
AU - Wingard, C. J.
C7 - 48
DB - Scopus
DO - 10.1186/S12989-016-0159-Z
IS - 1
KW - Aorta
Coronary artery
Myocardial infarction
Nanotoxicology
Pulmonary instillation
Serum cytokines
Animals
Gold
Heart Injuries
Inhalation Exposure
Lung
Male
Metal Nanoparticles
Particle Size
Rats
Silver
gamma interferon
interleukin 10
interleukin 13
interleukin 18
interleukin 1beta
interleukin 2
interleukin 5
interleukin 6
RANTES
silver nanoparticle
gold
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
cardiotoxicity
controlled study
heart muscle ischemia
in vivo study
male
nonhuman
particle size
priority journal
protein blood level
protein determination
rat
zeta potential
animal
chemically induced
chemistry
exposure
heart injury
lung
Sprague Dawley rat
M3 - Article
PY - 2016
ST - Impact of pulmonary exposure to gold core silver nanoparticles of different
size and capping agents on cardiovascular injury
TI - Impact of pulmonary exposure to gold core silver nanoparticles of different
size and capping agents on cardiovascular injury
85007489612&doi=10.1186%2fS12989-016-0159-
Z&partnerID=40&md5=2c141fd65191416baa2fb3f98a3b4dea
VL - 13
ID - 5623
ER -
TY - JOUR
AB - Bacterial adhesion to the calcium phosphate surface is a serious problem in
surgery. To prevent bacterial infection, the development of calcium-phosphate
cements (CPCs) with bactericidal properties is indispensable. The aim of this study
was to fabricate antibacterial CPCs and evaluate their biological properties.
Silver-containing tricalcium phosphate (Ag-TCP) microspheres consisting of α/β-TCP
phases were synthesized by an ultrasonic spray-pyrolysis technique. The powders
prepared were mixed with the setting liquid to fabricate the CPCs. The resulting
cements consisting of β-TCP and hydroxyapatite had a porous structure and wash-out
resistance. Additionally, silver and calcium ions could be released into the
culture medium from Ag-TCP cements for a long time accompanied by the dissolution
of TCP. These data showed the bioresorbability of the Ag-TCP cement. In vitro
antibacterial evaluation demonstrated that both released and immobilized silver
suppressed the growth of bacteria and prevented bacterial adhesion to the surface
of CPCs. Furthermore, histological evaluation by implantation of Ag-TCP cements
into rabbit tibiae exhibited abundant bone apposition on the cement without
inflammatory responses. These results showed that Ag-TCP cement has a good
antibacterial property and good biocompatibility. The present Ag-TCP cements are
promising for bone tissue engineering and may be used as antibacterial
biomaterials. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Honda, M.
AU - Kawanobe, Y.
AU - Nagata, K.
AU - Ishii, K.
AU - Matsumoto, M.
AU - Aizawa, M.
C7 - 3745
DB - Scopus
DO - 10.3390/ijms21113745
IS - 11
KW - Antimicrobial property
Calcium phosphate cement
Implant-related infection
Silver-containing tricalcium phosphate
Animals
Bone Cements
Calcium Phosphates
Hydroxyapatites
Male
Microspheres
Rabbits
Silver
Tibia
bone cement
calcium phosphate
hydroxyapatite
microsphere
silver
antiinfective agent
animal experiment
animal tissue
Article
bacterial cell wall
bacterial growth
bacterial viability
bacterium adherence
biocompatibility
biofilm
bone tissue
compressive strength
controlled study
dissolution
histology
hydrolysis
in vitro study
macrophage
male
nonhuman
ossification
osteoclast
precipitation
pyrolysis
rabbit model
tissue engineering
ultrasound
X ray diffraction
zone of inhibition
animal
chemistry
drug effect
Leporidae
surgery
tibia
M3 - Article
PY - 2020
ST - Bactericidal and bioresorbable calcium phosphate cements fabricated by
silver-containing tricalcium phosphate microspheres
TI - Bactericidal and bioresorbable calcium phosphate cements fabricated by
silver-containing tricalcium phosphate microspheres
85085543480&doi=10.3390%2fijms21113745&partnerID=40&md5=b6e069e259fda7fb95bba27bb44
61a3c
VL - 21
ID - 5263
ER -
TY - JOUR
AB - Although TiO2 nanopartides (NPs) as endocrine disruptors have been
demonstrated to be able to cross the blood testis barriers and induce reproductive
toxicity in male animals, whether the reproductive toxicity of male animals due to
exposure to endocrine disruptor TiO2 NPs is related to immunological dysfunction in
the testis remains not well understood. This study determined whether the
reproductive toxicity and immunological dysfunction induced by exposure to TiO2 NPs
is associated with activation or inhibition of TAM/TLR-mediated signal pathway in
mouse testis. The results showed that male mice exhibited significant reduction of
fertility, infiltration of inflammatory cells, rarefaction, apoptosis, and/or
necrosis of spermatogenic cells and Sertoli cells due to TiO2 NPs. Furthermore,
these were associated with decreased expression of Tyro3 (-18.16 to -66.6%), Axl (-
14.7 to -57.99%), Mer (-7.98 to -72.62%), and I kappa B (-11.25 to -63.16%),
suppression of cytokine signaling (SOCS) 1 (-21.99 to -73.8%) and SOCS3 (-8.11 to -
34.86%), and increased expression of Toll-like receptor (TLR)-3 (21.4-156.03%),
TLR-4 (37.0-109.87%), nuclear factor-kappa B (14.75-69.34%), interleukin (1L)-1
beta (46.15-123.08%), IL-6 (2.54-81.98%), tumor necrosis factor-alpha (6.95-
88.39%), interferon (IFN)-alpha (2.54-37.25%), and IFN-beta (10.19-80.56%), which
are involved in the immune environment in the testis. The findings showed that
reproductive toxicity of male mice induced by exposure to endocrine disruptor TiO2
NPs may be associated with biomarkers of impairment of immune environment or
dysfunction of TAM/TLR3-mediated signal pathway in mouse testitis. Therefore, the
potential risks to reproductive health should be attended, especially in those who
are occupationally exposed to TiO2 NPs.
AN - WOS:000368322900044
AU - Hong, F. S.
AU - Wang, Y. J.
AU - Zhou, Y. J.
AU - Zhang, Q.
AU - Ge, Y. S.
AU - Chen, M.
AU - Hong, J.
AU - Wang, L.
DA - JAN 13
DO - 10.1021/acs.jafc.5b05262
IS - 1
PY - 2016
SN - 0021-8561
1520-5118
SP - 346-355
ST - Exposure to TiO2 Nanoparticles Induces Immunological Dysfunction in Mouse
Testitis
T2 - JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
TI - Exposure to TiO2 Nanoparticles Induces Immunological Dysfunction in Mouse
Testitis
VL - 64
ID - 6555
ER -
TY - JOUR
AB - Emerging evidence indicates that a vicious cycle between inflammation and
microthrombosis catalyzes the pathogenesis of inflammatory bowel disease (IBD).
Over-stimulated inflammation triggers a coagulation cascade and leads to
microthrombosis, which further complicates the injury through tissue hypoxia and
ischemia. Herein, an injectable protein hydrogel with anti-thrombosis and anti-
inflammation competency is developed to impede this cycle, cross-linked by silver
ion mediated metal-ligand coordination and electronic interaction with sulfhydryl
functionalized bovine serum albumin and heparin, respectively. The ex vivo
experiments show that the hydrogel, HEP-Ag-BSA, exhibits excellent self-healing
ability, injectability, biocompatibility, and sustained drug release. HEP-Ag-BSA
also demonstrates anti-coagulation and anti-inflammation abilities via coagulation
analysis and lipopolysaccharide stimulation assay. The in vivo imaging confirms the
longer retention time of HEP-Ag-BSA at inflammatory sites than in normal mucosa
owing to electrostatic interactions. The in vivo study applying a mouse model with
colitis also reveals that HEP-Ag-BSA can robustly inhibit inflammatory
microthrombosis with reduced bleeding risk. This versatile protein hydrogel
platform can definitively hinder the "inflammation and microthrombosis" cycle,
providing a novel integrated approach against IBD.
AN - WOS:000791692000001
AU - Hong, L. W.
AU - Chen, G. X.
AU - Cai, Z. W.
AU - Liu, H.
AU - Zhang, C.
AU - Wang, F.
AU - Xiao, Z. Y.
AU - Zhong, J.
AU - Wang, L.
AU - Wang, Z. T.
AU - Cui, W. G.
C6 - MAY 2022
C7 - 2200281
DA - JUL
DO - 10.1002/advs.202200281
IS - 20
PY - 2022
SN - 2198-3844
ST - Balancing Microthrombosis and Inflammation via Injectable Protein Hydrogel
for Inflammatory Bowel Disease
T2 - ADVANCED SCIENCE
TI - Balancing Microthrombosis and Inflammation via Injectable Protein Hydrogel
VL - 9
ID - 6373
ER -
TY - JOUR
AB - Emerging evidence indicates that a vicious cycle between inflammation and
microthrombosis catalyzes the pathogenesis of inflammatory bowel disease (IBD).
Over-stimulated inflammation triggers a coagulation cascade and leads to
microthrombosis, which further complicates the injury through tissue hypoxia and
ischemia. Herein, an injectable protein hydrogel with anti-thrombosis and anti-
inflammation competency is developed to impede this cycle, cross-linked by silver
ion mediated metal-ligand coordination and electronic interaction with sulfhydryl
functionalized bovine serum albumin and heparin, respectively. The ex vivo
experiments show that the hydrogel, HEP-Ag-BSA, exhibits excellent self-healing
ability, injectability, biocompatibility, and sustained drug release. HEP-Ag-BSA
also demonstrates anti-coagulation and anti-inflammation abilities via coagulation
analysis and lipopolysaccharide stimulation assay. The in vivo imaging confirms the
longer retention time of HEP-Ag-BSA at inflammatory sites than in normal mucosa
owing to electrostatic interactions. The in vivo study applying a mouse model with
colitis also reveals that HEP-Ag-BSA can robustly inhibit inflammatory
microthrombosis with reduced bleeding risk. This versatile protein hydrogel
platform can definitively hinder the “inflammation and microthrombosis” cycle,
providing a novel integrated approach against IBD. © 2022 The Authors. Advanced
Science published by Wiley-VCH GmbH.
AU - Hong, L.
AU - Chen, G.
AU - Cai, Z.
AU - Liu, H.
AU - Zhang, C.
AU - Wang, F.
AU - Xiao, Z.
AU - Zhong, J.
AU - Wang, L.
AU - Wang, Z.
AU - Cui, W.
C7 - 2200281
DB - Scopus
DO - 10.1002/advs.202200281
IS - 20
KW - inflammatory bowel disease
microthrombosis
protein hydrogel
Animals
Drug Liberation
Hydrogels
Inflammation
Mice
Serum Albumin, Bovine
Coagulation
Diseases
Drug delivery
Mammals
Metal ions
Pathology
Proteins
Anti-inflammation
Catalyse
Injectables
Ischaemia
Metal ligands
Microthrombosis
Protein hydrogels
Silver ions
Tissue hypoxia
animal
drug release
hydrogel
inflammation
mouse
pharmacology
Biocompatibility
M3 - Article
PY - 2022
ST - Balancing Microthrombosis and Inflammation via Injectable Protein Hydrogel
T2 - Advanced Science
TI - Balancing Microthrombosis and Inflammation via Injectable Protein Hydrogel
85129398714&doi=10.1002%2fadvs.202200281&partnerID=40&md5=d0b98e36a82680148a64e9f86
4790927
VL - 9
ID - 5062
ER -
TY - JOUR
AB - Infectious diseases caused by new or unknown bacteria and viruses, such as
anthrax, cholera, tuberculosis and even COVID-19, are a major threat to humanity.
Thus, the development of new synthetic compounds with efficient antimicrobial
activity is a necessity. Herein, rationally designed novel multifunctional cationic
alternating copolymers were directly synthesized through a step-growth
polymerization reaction using a bivalent electrophilic cross-linker containing
disulfide bonds and a diamine heterocyclic ring. To optimize the activity of these
alternating copolymers, several different diamines and cross-linkers were explored
to find the highest antibacterial effects. The synthesized nanopolymers not only
displayed good to excellent antibacterial activity as judged by minimum inhibitory
concentration (MIC) and minimum bactericidal concentration (MBC) against
Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and
Escherichia coli, but also reduced the number of biofilm cells even at low
concentrations, without killing mammalian cells. Furthermore, in vivo experiments
using infected burn wounds in mice demonstrated good antibacterial activity and
stimulated wound healing, without causing systemic inflammation. These findings
suggest that the multifunctional cationic nanopolymers have potential as a novel
antibacterial agent for eradication of multidrug resistant bacterial infections. ©
2021
AU - Hooshmand, S. E.
AU - Ebadati, A.
AU - Hosseini, E. S.
AU - Vahabi, A. H.
AU - Oshaghi, M.
AU - Rahighi, R.
AU - Orooji, Y.
AU - Jahromi, M. A. M.
AU - Varma, R. S.
AU - Hamblin, M. R.
AU - Karimi, M.
C7 - 105550
DB - Scopus
DO - 10.1016/j.bioorg.2021.105550
KW - Alternating copolymer
Antibacterial activity
Antibiofilm activity
Cationic polymer
Nanomedicine
Wound healing
Amines
Animals
Bacteria
Biofilms
Burns
Cations
Cell Survival
COVID-19
Cross-Linking Reagents
Drug Resistance, Multiple, Bacterial
HEK293 Cells
Humans
Mice
Polymers
Wound Healing
1,4 diazabicyclo[2.2.2]octane
antibiotic agent
cation
cystamine
gentamicin
gold nanoparticle
graphene oxide
imidazole
interleukin 1
interleukin 6
ketamine
metal organic framework
metal organic framework 199
methenamine
piperazine
silver nanoparticle
spermine
unclassified drug
xylazine
amine
antiinfective agent
cross linking reagent
polymer
animal experiment
animal model
Article
bacterial strain
Bagg albino mouse
burn infection
cell regeneration
cell viability
colorimetry
comparative study
controlled study
cross linking
cytotoxicity
cytotoxicity assay
disulfide bond
drug design
drug potency
drug synthesis
electrophilicity
Escherichia coli
gel permeation chromatography
HEK293 cell line
hospital infection
human
human cell
in vitro study
in vivo study
mouse
MTT assay
nanoanalysis
nonhuman
nuclear magnetic resonance
particle size
polymerization
protein blood level
reaction time
wound healing
zeta potential
animal
bacterial infection
bacterium
biofilm
burn
cell survival
chemistry
complication
drug effect
M3 - Article
PY - 2022
ST - Antibacterial, antibiofilm, anti-inflammatory, and wound healing effects of
nanoscale multifunctional cationic alternating copolymers
T2 - Bioorganic Chemistry
TI - Antibacterial, antibiofilm, anti-inflammatory, and wound healing effects of
nanoscale multifunctional cationic alternating copolymers
85121271231&doi=10.1016%2fj.bioorg.2021.105550&partnerID=40&md5=1a333915523d12e042d
9c1090295c84b
VL - 119
ID - 5096
ER -
TY - JOUR
AB - Background: Multiple sclerosis (MS) is often accompanied by optic nerve
inflammation. And some patients experience permanent vision loss. We examined if
the grade of optic nerve infiltration and demyelination affects the severity of
clinical signs in an experimental autoimmune encephalomyelitis (EAE) model. The
loss of retinal ganglion cells (RGC) and alterations in glia activity were also
investigated.Methods: C57BL/6 mice were immunized with peptide MOG35-55 in complete
Freund's adjuvant (CFA) and controls received PBS in CFA. Then 23 days post
immunization eyes were prepared for flatmounts and stained with Nissl to evaluated
neuronal density. Clinical EAE symptoms as well as cell infiltration and
demyelination in the optic nerve were examined. Retinal sections were stained with
hematoxylin and eosin and silver stain. Immunohistochemistry was used to label RGCs
(Brn-3a), apoptotic cells (caspase 3), macroglia (glial fibrillary acidic protein
(GFAP)), microglia (Iba1), macrophages (F 4/80) and interleukin-6 (IL-6) secretion.
Results: EAE symptoms started at day 8 and peaked at day 15. Cell infiltrations (P
= 0.0047) and demyelination (P = 0.0018) of EAE nerves correlated with the clinical
score (r > 0.8). EAE led to a significant loss of RGCs (P< 0.0001). Significantly
more caspase 3+ cells were noted in these animals (P = 0.0222). They showed an
increased expression of GFAP (P< 0.0002) and a higher number of microglial cells
(P< 0.0001). Also more macrophages and IL-6 secretion were observed in EAE mice.
Conclusions: MOG immunization leads to optic neuritis and RGC loss. EAE severity is
related to the severity of optic nerve inflammation and demyelination. EAE not only
affects activation of apoptotic signals, but also causes a glial response in the
retina. © 2013 Horstmann et al.; licensee BioMed Central Ltd.
AU - Horstmann, L.
AU - Schmid, H.
AU - Heinen, A. P.
AU - Kurschus, F. C.
AU - Dick, H. B.
AU - Joachim, S. C.
C7 - 888
DB - Scopus
DO - 10.1186/1742-2094-10-120
KW - Apoptosis
Demyelination
EAE
Glia
MOG
Multiple sclerosis
Optic nerve
Optic neuritis
Retina
RGC
Animals
Demyelinating Diseases
Encephalomyelitis, Autoimmune, Experimental
Inflammation
Male
Mice
Mice, Inbred C57BL
Neuroglia
Optic Neuritis
Retinal Ganglion Cells
caspase 3
cell protein
interleukin 6
myelin oligodendrocyte glycoprotein
protein F4/80
protein Iba1
transcription factor POU4F1
unclassified drug
allergic encephalomyelitis
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
cell density
cell infiltration
cell labeling
cell loss
controlled study
cytokine release
demyelination
disease severity
histopathology
macroglia
male
mouse
nonhuman
optic neuritis
protein expression
retina ganglion cell
silver staining
M3 - Article
PY - 2013
ST - Inflammatory demyelination induces glia alterations and ganglion cell loss in
the retina of an experimental autoimmune encephalomyelitis model
T2 - Journal of Neuroinflammation
TI - Inflammatory demyelination induces glia alterations and ganglion cell loss in
the retina of an experimental autoimmune encephalomyelitis model
84884923616&doi=10.1186%2f1742-2094-10-
120&partnerID=40&md5=ef6d3cb74c55b9e8e2a4f903b8755883
VL - 10
ID - 5730
ER -
TY - JOUR
AB - Impaired wound healing is a common complication associated with diabetes with
complex pathophysiological underlying mechanisms and often necessitates amputation.
With the advancement in laser technology, irradiation of these wounds with low-
intensity laser irradiation (LILI) or phototherapy, has shown a vast improvement in
wound healing. At the correct laser parameters, LILI has shown to increase
migration, viability, and proliferation of diabetic cells in vitro; there is a
stimulatory effect on the mitochondria with a resulting increase in adenosine
triphosphate (ATP). In addition, LILI also has an anti-inflammatory and protective
effect on these cells. In light of the ever present threat of diabetic foot ulcers,
infection, and amputation, new improved therapies and the fortification of wound
healing research deserves better prioritization. In this review we look at the
complications associated with diabetic wound healing and the effect of laser
irradiation both in vitro and in vivo in diabetic wound healing. © 2014 Nicolette
N. Houreld.
AU - Houreld, N. N.
C7 - 398412
DB - Scopus
DO - 10.1155/2014/398412
KW - Animals
Diabetes Complications
Humans
Laser Therapy, Low-Level
Phototherapy
Treatment Outcome
Wound Healing
Wounds and Injuries
antibiotic agent
collagen
cyclic AMP
cytochrome c oxidase
nitric oxide
procollagen
sulfadiazine silver
vasculotropin
aerobic metabolism
antibiotic therapy
apoptosis
calcium cell level
cell death
cell migration
cell proliferation
cell viability
collagen synthesis
debridement
diabetes mellitus
diabetic foot
human
hyperbaric oxygen
hyperglycemia
hypoxemia
in vitro study
in vivo study
leg revascularization
light emitting diode
low level laser therapy
nonhuman
oxidative stress
pathogenesis
phototherapy
randomized controlled trial (topic)
review
upregulation
wound infection
animal
procedures
treatment outcome
wound healing
M3 - Review
PY - 2014
ST - Shedding light on a new treatment for diabetic wound healing: A review on
phototherapy
T2 - The Scientific World Journal
TI - Shedding light on a new treatment for diabetic wound healing: A review on
phototherapy
84893166567&doi=10.1155%2f2014%2f398412&partnerID=40&md5=ce7abf9c3f32e7bd9702211114
e72836
VL - 2014
ID - 5624
ER -
TY - JOUR
AB - Recently, the incidence of chronic diabetic wounds increases continuously,
and the existing clinical treatment is less effective. Thus, it is an urgent need
to solve these problems for better clinical treatment effects. Herein, we prepared
a brand-new tailored recombinant human collagen type III (rhCol III) and
constructed a multifunctional microenvironment-responsive hydrogel carrier based on
multifunctional antibacterial nanoparticles (PDA@Ag NPs) and our tailored rhCol
III. The multifunctional smart hydrogel disintegrated quickly at the chronic
diabetic wound sites and achieved the programed on-demand release of different
therapeutic substances. The first released PDA@Ag NPs showed great antibacterial
properties against S. aureus and E. coli. They could kill bacteria rapidly, and
also showed antioxidant and anti-inflammatory effects at the wound site. The
subsequent release of our tailored rhCol III could promote the proliferation and
migration of mouse fibroblasts and endothelial cells during the proliferation and
remodeling process of wound healing. Relevant results showed that the
multifunctional smart hydrogel could promote the expression levels of basic
fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF),
decrease the inflammatory response, accelerate the deposition of collagen and
increase cell proliferation and angiogenesis, thereby speeding up the healing of
infected chronic wounds. In a word, the hydrogel, which took into consideration the
complex microenvironment at the wound site and multi-stage healing process, could
achieve programmed and responsive release of different therapeutic substances to
meet the treatment needs in different wound healing stages. More importantly, our
work illustrated the great application potential of our brand-new rhCol III in
promoting chronic wound repair and regeneration. This journal is © The Royal
Society of Chemistry.
AU - Hu, C.
AU - Liu, W.
AU - Long, L.
AU - Wang, Z.
AU - Yuan, Y.
AU - Zhang, W.
AU - He, S.
AU - Wang, J.
AU - Yang, L.
AU - Lu, L.
AU - Wang, Y.
DB - Scopus
DO - 10.1039/d1tb02170b
IS - 47
KW - Animals
Cell Line
Collagen Type III
Diabetes Complications
Drug Liberation
Escherichia coli
Hemolysis
Humans
Hydrogels
Indoles
Metal Nanoparticles
Mice
Polymers
Rabbits
Rats
Silver
Wound Healing
Wound Infection
Cell culture
Cell proliferation
Collagen
Endothelial cells
Fibroblasts
Repair
Tissue regeneration
antiinfective agent
collagen type 3
indole derivative
metal nanoparticle
polydopamine
polymer
recombinant protein
silver
Chronic wounds
Clinical treatments
Diabetic wounds
Microenvironments
Recombinant human collagen
Smart hydrogels
Treatment effects
Wound healing
Wound site
animal
cell line
chemistry
diabetic complication
drug effect
drug release
hemolysis
human
hydrogel
Leporidae
mouse
rat
toxicity
wound healing
wound infection
M3 - Article
PY - 2021
SP - 9684-9699
ST - Microenvironment-responsive multifunctional hydrogels with spatiotemporal
sequential release of tailored recombinant human collagen type III for the rapid
repair of infected chronic diabetic wounds
T2 - Journal of Materials Chemistry B
TI - Microenvironment-responsive multifunctional hydrogels with spatiotemporal
sequential release of tailored recombinant human collagen type III for the rapid
repair of infected chronic diabetic wounds
85121136617&doi=10.1039%2fd1tb02170b&partnerID=40&md5=a16205bb46bcac1197adf95c505ac
f2d
VL - 9
ID - 5171
ER -
TY - JOUR
AB - A novel injectable hydrogel dressing (GA@AgNPs-SA) with long-term
antimicrobial effect is developed that can accelerate the closure of bacteria-
infected wounds. The hydrogel dressing was prepared by cross-linking sodium
alginate molecular chains and gallic acid functionalized silver nanoparticles
(GA@AgNPs) via calcium ions to form a three-dimensional network. The hydrogel
dressing demonstrates excellent biocompatibility and can achieve a sustainable
release of silver ions, ensuring a long-term antibacterial activity and inhibiting
biofilm formation. Moreover, an in vivo study demonstrates that the GA@AgNPs-SA
hydrogel can effectively decrease the expression of IL-6 and TNF-α to alleviate the
inflammatory response, and promote angiogenesis by upregulating CD31, α-SMA and
VEGF expression, thus significantly accelerating the repair of infected wounds.
Given these interesting properties, this antibacterial hydrogel has great potential
for application in the clinical care of bacteria-infected wounds. © 2023
AU - Hu, Q.
AU - Nie, Y.
AU - Xiang, J.
AU - Xie, J.
AU - Si, H.
AU - Li, D.
AU - Zhang, S.
AU - Li, M.
AU - Huang, S.
C7 - 123691
DB - Scopus
DO - 10.1016/j.ijbiomac.2023.123691
KW - Injectable hydrogel
Plant polyphenol-functionalized silver nanoparticles
Wound healing
Alginates
Hydrogels
Ions
Metal Nanoparticles
Silver
Wound Healing
alginic acid
alpha smooth muscle actin
calcium ion
gallic acid
hydrogel
interleukin 6
polyphenol
silver nanoparticle
silver nitrate
vasculotropin
antiinfective agent
ion
metal nanoparticle
silver
angiogenesis
animal experiment
animal tissue
Article
bacterial infection
biocompatibility
biofilm
cell viability
Escherichia coli
inflammation
male
nonhuman
wound healing
wound infection
zone of inhibition
pharmacology
M3 - Article
PY - 2023
ST - Injectable sodium alginate hydrogel loaded with plant polyphenol-
functionalized silver nanoparticles for bacteria-infected wound healing
TI - Injectable sodium alginate hydrogel loaded with plant polyphenol-
functionalized silver nanoparticles for bacteria-infected wound healing
85148373889&doi=10.1016%2fj.ijbiomac.2023.123691&partnerID=40&md5=eeca901c5d1f34af1
0a0a574b1ff9fb8
VL - 234
ID - 5011
ER -
TY - JOUR
AB - With the rapid development of nanotechnology, there is a growing interest on
the application of nanoparticles in various fields such as photonics, catalysis,
magnetics, and biotechnology including cosmetics, pharmaceutics, and medicines.
However, little is known about their potential toxicity to human health. Owing to
their special properties, nanoparticles have the capacity to bypass the blood-brain
barrier (BBB). However, the toxic effects of nanoparticles on central nervous
system (CNS) function are still lacking. And the interactions of nanoparticles with
the cells and tissues in CNS are poorly understood. Thus, neurotoxicity induced by
nanoparticles is still a new topic that requires more attention. In this review, we
summarized the pathways by which the nanoparticles could enter into the CNS and the
recent investigations on the neurotoxicity of nanoparticles both in vitro and in
vivo, as well as the potential mechanisms. Furthermore, the future direction in the
neurotoxicity studies of nanoparticles is also discussed. © 2010 Elsevier B.V.
AU - Hu, Y. L.
AU - Gao, J. Q.
DB - Scopus
DO - 10.1016/j.ijpharm.2010.04.026
IS - 1-2
KW - Nanoparticles
Neurotoxicity
Polymer nanocarrier
Animals
Blood-Brain Barrier
Brain
Humans
Nanotechnology
copper nanoparticle
CXCL9 chemokine
dihydroxyphenylacetic acid
dopamine
doxorubicin
ferric oxide nanoparticle
fullerene
homovanillic acid
interleukin 1beta
leucine enkephalin[2 dextro alanine 6 arginine]
metal nanoparticle
nanoparticle
nerve cell adhesion molecule
polysorbate 80
silver nanoparticle
titanium oxide nanoparticle
ultrafine carbon black nanoparticle
unclassified drug
blood brain barrier
cell line
cell viability
central nervous system function
cytotoxicity
drug penetration
drug solubility
drug stability
drug targeting
glioblastoma
human
in vitro study
in vivo study
lipid peroxidation
microarray analysis
nerve cell lesion
neurotoxicity
nonhuman
oxidative stress
particle size
physical chemistry
priority journal
review
toxicokinetics
animal
brain
metabolism
nanotechnology
procedures
toxicity
M3 - Review
PY - 2010
SP - 115-121
ST - Potential neurotoxicity of nanoparticles
TI - Potential neurotoxicity of nanoparticles
77953717599&doi=10.1016%2fj.ijpharm.2010.04.026&partnerID=40&md5=2284af8e982ff148b5
e928ce6c80b0b4
VL - 394
ID - 5755
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have antibacterial characteristics, and
currently are applied in Ag-containing products. This study found neural cells can
uptake 3-5. nm AgNPs, and investigated the potential effects of AgNPs on gene
expression of inflammation and neurodegenerative disorder in murine brain ALT
astrocytes, microglial BV-2 cells and neuron N2a cells. After AgNPs (5, 10, 12.5.
μg/ml) exposure, these neural cells had obviously increased IL-1β secretion, and
induced gene expression of C-X-C motif chemokine 13 (CXCL13), macrophage receptor
with collagenous structure (MARCO) and glutathione synthetase (GSS) for
inflammatory response and oxidative stress neutralization. Additionally, this study
found amyloid-β (Aβ) plaques for pathological feature of Alzheimer's disease (AD)
deposited in neural cells after AgNPs treatment. After AgNPs exposure, the gene
expression of amyloid precursor protein (APP) was induced, and otherwise,
neprilysin (NEP) and low-density lipoprotein receptor (LDLR) were reduced in neural
cells as well as protein level. These results suggested AgNPs could alter gene and
protein expressions of Aβ deposition potentially to induce AD progress in neural
cells. It's necessary to take notice of AgNPs distribution in the environment. ©
2014 The Authors.
AU - Huang, C. L.
AU - Hsiao, I. L.
AU - Lin, H. C.
AU - Wang, C. F.
AU - Huang, Y. J.
AU - Chuang, C. Y.
DB - Scopus
DO - 10.1016/j.envres.2014.11.006
KW - Alzheimer 's disease
Gene expression
Inflammation
Neurodegenerative disorder
Silver nanoparticle
Animals
Brain
Gene Expression
Metal Nanoparticles
Mice
Silver
CXCL13 chemokine
glutathione synthase
interleukin 1beta
low density lipoprotein receptor
membrane metalloendopeptidase
silver nanoparticle
metal nanoparticle
silver
brain
gene expression
neurology
oxidation
particle size
physiological response
protein
Alzheimer disease
animal cell
Article
astrocyte
brain nerve cell
cell proliferation
controlled study
cytokine production
cytotoxicity
inflammation
microglia
mouse
nerve degeneration
nonhuman
oxidative stress
protein induction
Western blotting
animal
chemistry
cytology
drug effects
genetics
M3 - Article
PY - 2015
SP - 253-263
ST - Silver nanoparticles affect on gene expression of inflammatory and
neurodegenerative responses in mouse brain neural cells
TI - Silver nanoparticles affect on gene expression of inflammatory and
neurodegenerative responses in mouse brain neural cells
84911365647&doi=10.1016%2fj.envres.2014.11.006&partnerID=40&md5=5c907ed34e1d9134799
3c2e60b678b83
VL - 136
ID - 5642
ER -
TY - JOUR
AB - Histologic investigations have demonstrated that root canal sealers can
induce mild to severe inflammatory alternations. A recently identified tumor
necrosis factor family molecule, receptor activator of nuclear factor-κB ligand
(RANKL), plays a critical role in the development of osteoclasts that result in
bone resorption. The aim of this study was to investigate the effects of root canal
sealers AH26, Canals, and N2 on the expression of RANKL in human osteoblast cell
line U2OS cells. Freshly mixed materials were filled in glass rings (4 mm height
and 10 mm in diameter) and eluted in 10 mL of culture medium for 1 day.
Subsequently, various dilutions (final dilution: 1/2, 1/4, and 1/8) of these
extraction media were prepared for this study. Cytotoxicity was measured by the
propidium iodide fluorescence assay. The expression of RANKL was measured by
reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent
assay. The results showed that AH26, Canals, and N2 were cytotoxic to U2OS cells in
a concentration-dependent manner (P < .05). The exposure of U2OS cells to root
canal sealers resulted in the up-regulation of RANKL mRNA and protein expression (P
< .05). The expression of RANKL was up-regulated by root canal sealers in the
following order: N2 > AH26 > Canals. Taken together, the activation of RANKL might
play an important role in the pathogenesis of periapical bone destruction induced
by root canal sealers. © 2009 American Association of Endodontists.
AU - Huang, F. M.
AU - Lee, S. S.
AU - Yang, S. F.
AU - Chang, Y. C.
DB - Scopus
DO - 10.1016/j.joen.2008.11.020
IS - 3
KW - Osteoblastic cells
RANKL
root canal sealers
Apoptosis
Bismuth
Cell Line, Tumor
Cell Survival
Drug Combinations
Epoxy Resins
Eugenol
Fluorescent Dyes
Formaldehyde
Gene Expression
Humans
Osteoblasts
RANK Ligand
RNA, Messenger
Silver
Titanium
Up-Regulation
Zinc Oxide
bismuth
canals sealer
epoxy resin
eugenol
fluorescent dye
formaldehyde
messenger RNA
N2 Dental Cement
osteoclast differentiation factor
silver
titanium
zinc oxide
zinc oxide eugenol
apoptosis
article
biosynthesis
cell survival
dose response
drug combination
drug effect
gene expression
genetics
human
metabolism
osteoblast
tumor cell line
upregulation
M3 - Article
PY - 2009
SP - 363-366
ST - Up-regulation of Receptor Activator Nuclear Factor-Kappa B Ligand Expression
by Root Canal Sealers in Human Osteoblastic Cells
TI - Up-regulation of Receptor Activator Nuclear Factor-Kappa B Ligand Expression
by Root Canal Sealers in Human Osteoblastic Cells
60649102477&doi=10.1016%2fj.joen.2008.11.020&partnerID=40&md5=258b06823ccf7e0dc457c
867e50c939e
VL - 35
ID - 5832
ER -
TY - JOUR
AB - Aim The purpose of this study was to determine the cytotoxicity of three
different types of root canal sealer on human periodontal ligament (PDL) cells and
a permanent hamster cell line (V79 cells). Methodology Set specimens from two resin
based sealers (AH26 and AHPlus), three zinc oxide-eugenol-based sealers (Canals,
Endomethansone and N2) and one calcium hydroxide-based sealer (Sealapex) were
eluted with culture medium for 1, 2, 3 and 7 days. Cytotoxicity was judged using
tetrazolium bromide reduction assay on human primary PDL cells and V79 cells
derived from a Chinese hamster. Results The results showed that elutes from resin-
based, zinc oxide-eugenol-based, and calcium hydroxide-based sealers were cytotoxic
to primary human PDL cultures and V79 cells. Calcium hydroxide-based sealer was the
least toxic sealer amongst the chemicals tested in both cultures. The cytotoxic
response decreased in an order of N2 > Endomethansone > AH26 > AHplus > Canals >
Sealapex. Conclusions The sensitivity of toxicity depended on the materials tested
and the cell culture system used. Thus, the use of both permanent and primary cells
is recommended for screening of the cytotoxic effects of root canal sealers. In
addition, the results confirmed that root canal sealers constantly dissolve when
exposed to an aqueous environment for extended periods, possibly causing moderate
or severe cytotoxic reactions. Use of calcium hydroxide-based material as a root
canal sealer initially may result in a more favourable response to periradicular
tissues.
AU - Huang, F. M.
AU - Tai, K. W.
AU - Chou, M. Y.
AU - Chang, Y. C.
DB - Scopus
DO - 10.1046/j.1365-2591.2002.00459.x
IS - 2
KW - Cytotoxicity
Permanent cell line
Primary culture
Root canal sealer
Analysis of Variance
Animals
Bismuth
Calcium Hydroxide
Cells, Cultured
Cricetinae
Dexamethasone
Drug Combinations
Epoxy Resins
Fibroblasts
Formaldehyde
Humans
Hydrocortisone
Methenamine
Periodontal Ligament
Resin Cements
Salicylates
Silver
Thymol
Titanium
AH Plus
bismuth
calcium hydroxide
canals sealer
dexamethasone
drug derivative
epoxy resin
formaldehyde
hydrocortisone
methenamine
resin cement
salicylic acid derivative
sealapex
silver
thymol
titanium
zinc oxide eugenol
analysis of variance
animal
article
cell culture
cytology
drug combination
drug effect
fibroblast
hamster
human
nonparametric test
periodontal ligament
M3 - Article
PY - 2002
SP - 153-158
ST - Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root
canal sealers on human periodontal ligament cells and permanent V79 cells
TI - Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root
canal sealers on human periodontal ligament cells and permanent V79 cells
0036480135&doi=10.1046%2fj.1365-
2591.2002.00459.x&partnerID=40&md5=b9c0dfe085d02062e6637cc0887e47b2
VL - 35
ID - 5825
ER -
TY - JOUR
AB - Self-assemblies of bioactively natural compounds into supramolecular
hydrogels without structural modifications are of interest to improve their
sustained releases and bioavailabilities in vivo. However, it is still a formidable
challenge to dig out such a naturally small molecule with a meticulous structure
which can be self-assembled to form a hydrogel for biomedical applications. Here, a
new hydrogel consisting only of gallic acid (GA) via π–π stacking and hydrogen bond
interactions, whereas none of GA analogues can form the similar supramolecular
hydrogels, is reported. This interesting phenomenon is intriguing to further
investigate the potential applications of GA hydrogels in wound healing. Notably,
this GA hydrogel has rod-like structures with lengths varying from 10 to 100 µm.
The biocompatibility and antibacterial tests prove that this well-assembled GA
hydrogel has no cytotoxicity and excellent antibacterial activities against
Escherichia coli and Staphylococcus aureus. Moreover, the GA hydrogel can
significantly accelerate the process of wound healing with or without bacterial
infections by mediation of inflammation signaling pathways. It is believed that the
current study may shed a new light on the design of a supramolecular hydrogel based
on self-assemblies of naturally small molecules to improve their bioavailabilities
and diversify their uses in biomedical applications. © 2022 Wiley-VCH GmbH.
AU - Huang, H.
AU - Gong, W.
AU - Wang, X.
AU - He, W.
AU - Hou, Y.
AU - Hu, J.
C7 - 2102476
DB - Scopus
DO - 10.1002/adhm.202102476
IS - 12
polyphenols
self-assembly
supramolecular hydrogels
wound healing
Escherichia coli
Hydrogels
Wound Healing
Biochemistry
Biocompatibility
Hydrogen bonds
Molecules
Self assembly
Supramolecular chemistry
antiinfective agent
cinnamic acid
claudin 1
ellagic acid
high mobility group B1 protein
interleukin 6
nanofiber
polyvinylidene fluoride
protocatechualdehyde
protocatechuic acid
self assembled nanoparticle
silver nanoparticle
Fibrillar networks
Gallic acids
Natural compounds
Polyphenols
Small molecules
Supramolecular hydrogels
Wound healing
angiogenesis
Article
bacterial viability
bioavailability
biocompatibility
biodegradability
biofilm
cell proliferation
cell viability
chemoluminescence
circular dichroism
colony forming unit
cytotoxicity
drug synthesis
electrochemiluminescence
immunofluorescence
molecular library
MTT assay
protein expression
signal transduction
supramolecular chemistry
tissue regeneration
Western blotting
wound healing assay
zone of inhibition
chemistry
hydrogel
pharmacology
M3 - Article
PY - 2022
ST - Self-Assembly of Naturally Small Molecules into Supramolecular Fibrillar
Networks for Wound Healing
TI - Self-Assembly of Naturally Small Molecules into Supramolecular Fibrillar
Networks for Wound Healing
85128961876&doi=10.1002%2fadhm.202102476&partnerID=40&md5=3da64a5eb911bad5f18c5f161
cd351fc
VL - 11
ID - 5106
ER -
TY - JOUR
AB - (Arginine-Glycine-Aspartic)-methoxy polyethylene glycol-(1,2-distearoyl-sn-
glycero-3-phosphoethanolaMine-N) (abbreviation: RGD-PEG2000-DSPE or RGD-PD) was
successfully synthesized and verified by H-1-NMR and MALDI-TOF MS. Polyethylene
glycol-poly-L-lysine/RGD-PD/phospholipid/calcium phosphate nanoparticles
(PEG-PLL/RGD-PD/PL/CaP NPs or MNPs) were prepared using a novel, simple method
conducted at room temperature. Transmission electron microscopy (TEM) analysis
showed that the MNPs were spheres of uniform size, with a diameter of similar to 30
nm, and smooth surface. Thermogravimetric analysis (TGA) revealed that the PEG-
PLL/RGD-PD/PL micelle was packed in the CaP shell. MNPs had little effect on
hemolysis, coagulation, cardiac oxidative stress, inflammatory response and DNA
damage, indicating negligible cytotoxicity in vitro and in vivo. Experiments in
Zebrafish indicated that the MNPs neither affected the survival rate and heartbeat
rate, nor induced malformation and apoptosis during embryogenesis. In conclusion,
these results demonstrate that the newly-developed MNPs have good biocompatibility
and a great potential as drug and gene carrier.
AN - WOS:000425869200006
AU - Huang, J. W.
AU - Zhang, X. Y.
AU - Wu, Z. H.
AU - Wu, Y. F.
AU - Wu, X. J.
AU - Wang, Y. G.
AU - Jiang, H.
AU - Ma, J.
AU - He, Z. L.
DA - JAN
DO - 10.1166/jbn.2018.2460
IS - 1
PY - 2018
SN - 1550-7033
1550-7041
SP - 98-113
ST - Preparation and Biocompatibility Evaluation of
PEG-PLL/RGD-PEG-DSPE/Phospholipid/CaP Nanoparticles
TI - Preparation and Biocompatibility Evaluation of
PEG-PLL/RGD-PEG-DSPE/Phospholipid/CaP Nanoparticles
VL - 14
ID - 6266
ER -
TY - JOUR
AB - Xanthan gum is a high molecular weight polysaccharide biocompatible to
biological systems, so its products promise high potential in medicine. In this
study, we crosslinked xanthan gum with citric acid to develop a transparent film
for protecting the wound. Silver nanoparticles (AgNPs) are incorporated into the
film to enhance the antimicrobial property of our biomaterial. This paper discussed
the characteristics and manufacturing of this nanocomposite dressing. The safety of
the dressing was studied using fibroblasts (L929) by the method of 3-(4,5-
dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and staining of
ethidium homodimer (PI) and calcein AM. The bacterial inhibition test and
application of the dressing to nonhealing wounds infected with methicillin-
resistant S. aureus (MRSA) were performed to evaluate the antibacterial effects in
vitro and in vivo, respectively. The results indicated that the dressing could
restrict the formation of biofilms, reduce inflammatory reactions, and promote the
angiogenesis of granulation tissues in infectious wounds. Therefore, this dressing
has a great advantage over traditional clinical products especially when
administered under the condition of infections or for the purpose of infection
prevention. © 2017 Jinjian Huang et al.
AU - Huang, J.
AU - Ren, J.
AU - Chen, G.
AU - Deng, Y.
AU - Wang, G.
AU - Wu, X.
C7 - 6802397
DB - Scopus
DO - 10.1155/2017/6802397
Cell culture
Metal nanoparticles
Nanoparticles
Xanthan gum
Bacterial inhibition
Ethidium homodimer
High molecular weight
Silver
M3 - Article
PY - 2017
ST - Evaluation of the Xanthan-Based Film Incorporated with Silver Nanoparticles
for Potential Application in the Nonhealing Infectious Wound
T2 - Journal of Nanomaterials
TI - Evaluation of the Xanthan-Based Film Incorporated with Silver Nanoparticles
for Potential Application in the Nonhealing Infectious Wound
85029804801&doi=10.1155%2f2017%2f6802397&partnerID=40&md5=76cde43d58729b8bb32b91e44
3f7e15c
VL - 2017
ID - 5473
ER -
TY - JOUR
AB - Neutrophil extracellular traps (NETs) stick to bacteria and prevent
infections in vivo, whose activation is upon inflammatory stimuli along with the
sudden increase of reactive oxygen species (ROS). Nevertheless, the risky over
activation in NETosis may result in deleterious outcome. A big challenge in using
NETs for therapeutics is to synthesize an artificial system that can function as
NETs in vivo. Here, we developed an in vivo supramolecular assembly system to
imitate the innate immune process of NETs to inhibit methicillin-resistant
staphylococcus epidermidis (MRSE) infection. Our synthesized small molecules
undergo oxidation to form supramolecular nanofibers at inflammatory loci. The in
situ formed nanofibers network efficiently traps MRSE cells and prevent them from
aggressive dissemination. The extended interactions between nanofibers and bacteria
directly result in the death of MRSE via the transcriptomes alterations. In
clinically relevant models (intraperitoneal infection and catheter implantation),
our supramolecular nets show significant antibacterial activity, yielding a three
times efficacy comparing to vancomycin. The spontaneous consumption of ROS and the
formation of antibacterial networks create a steady negative feedback system to
combat bacterial infections.
AN - WOS:000536893800007
AU - Huang, Z. T.
AU - Liu, Y.
AU - Wang, L.
AU - Ali, A.
AU - Yao, Q. X.
AU - Jiang, X. Y.
AU - Gao, Y.
C7 - 120124
DA - SEP
DO - 10.1016/j.biomaterials.2020.120124
PY - 2020
SN - 0142-9612
1878-5905
ST - Supramolecular assemblies mimicking neutrophil extracellular traps for MRSE
infection control
T2 - BIOMATERIALS
TI - Supramolecular assemblies mimicking neutrophil extracellular traps for MRSE
infection control
VL - 253
ID - 6805
ER -
TY - JOUR
AB - Background: Nanosilver is one of the most commonly used engineered
nanomaterials (ENMs). In our study we focused on assessing the size-dependence of
the toxicity of nanosilver (Ag ENMs), utilising materials of three sizes (50, 80
and 200 nm) synthesized by the same method, with the same chemical composition,
charge and coating. Methods: Uptake and localisation (by Transmission Electron
Microscopy), cell proliferation (Relative growth activity) and cytotoxic effects
(Plating efficiency), inflammatory response (induction of IL-8 and MCP-1 by Enzyme
linked immune sorbent assay), DNA damage (strand breaks and oxidised DNA lesions by
the Comet assay) were all assessed in human lung carcinoma epithelial cells (A549),
and the mutagenic potential of ENMs (Mammalian hprt gene mutation test) was
assessed in V79-4 cells as per the OECD protocol. Detailed physico-chemical
characterization of the ENMs was performed in water and in biological media as a
prerequisite to assessment of their impacts on cells. To study the relationship
between the surface area of the ENMs and the number of ENMs with the biological
response observed, Ag ENMs concentrations were recalculated from μg/cm2 to ENMs
cm2/cm2 and ENMs/cm2. Results: Studied Ag ENMs are cytotoxic and cytostatic, and
induced strand breaks, DNA oxidation, inflammation and gene mutations. Results
expressed in mass unit [μg/cm2] suggested that the toxicity of Ag ENMs is size
dependent with 50 nm being most toxic. However, re-calculation of Ag ENMs
concentrations from mass unit to surface area and number of ENMs per cm2
highlighted that 200 nm Ag ENMs, are the most toxic. Results from hprt gene
mutation assay showed that Ag ENMs 200 nm are the most mutagenic irrespective of
the concentration unit expressed. Conclusion: We found that the toxicity of Ag ENMs
is not always size dependent. Strong cytotoxic and genotoxic effects were observed
in cells exposed to Ag ENMs 50 nm, but Ag ENMs 200 nm had the most mutagenic
potential. Additionally, we showed that expression of concentrations of ENMs in
mass units is not representative. Number of ENMs or surface area of ENMs (per cm2)
seem more precise units with which to compare the toxicity of different ENMs. © Huk
et al.; licensee BioMed Central.
AU - Huk, A.
AU - Izak-Nau, E.
AU - Reidy, B.
AU - Boyles, M.
AU - Duschl, A.
AU - Lynch, I.
AU - Dušinska, M.
C7 - 65
DB - Scopus
DO - 10.1186/s12989-014-0065-1
IS - 1
KW - Cytotoxicity
DNA damage
Inflammation
Mutagenicity
Silver nanomaterials
Size-related nanomaterial toxicity
Uptake and localisation
Animals
Cell Line, Tumor
Cell Proliferation
Cell Shape
Cell Survival
Chemokine CCL2
Cricetinae
DNA Damage
Humans
Interleukin-8
Lung
Metal Nanoparticles
Mutation
Oxidative Stress
Particle Size
Risk Assessment
Silver
Surface Properties
Time Factors
Mammalia
hypoxanthine phosphoribosyltransferase
interleukin 8
nanomaterial
nanosilver
unclassified drug
autacoid
CCL2 protein, human
IL8 protein, human
metal nanoparticle
silver
animal cell
Article
cell proliferation
cell structure
Chinese hamster
controlled study
crystal structure
cytokine release
cytostasis
cytotoxicity
DNA oxidation
DNA strand breakage
gene mutation
genotoxicity
HPRT gene
human
human cell
IC50
mutational analysis
nanoanalysis
nanofabrication
nanotoxicology
nonhuman
oxidation
particle size
priority journal
protein induction
animal
cell shape
cell survival
chemistry
dose response
drug effects
hamster
immunology
lung
metabolism
mutation
oxidative stress
pathology
risk assessment
surface property
time factor
tumor cell line
M3 - Article
PY - 2014
ST - Is the toxic potential of nanosilver dependent on its size?
TI - Is the toxic potential of nanosilver dependent on its size?
84924264193&doi=10.1186%2fs12989-014-0065-
1&partnerID=40&md5=9523b9e0f2bbe5599dd15d2bcb53ad01
VL - 11
ID - 5568
ER -
TY - JOUR
AB - Background: Nanosilver is one of the most commonly used engineered
nanomaterials (ENMs). In our study we focused on assessing the size-dependence of
the toxicity of nanosilver (Ag ENMs), utilising materials of three sizes (50, 80
and 200 nm) synthesized by the same method, with the same chemical composition,
charge and coating. Methods: Uptake and localisation (by Transmission Electron
Microscopy), cell proliferation (Relative growth activity) and cytotoxic effects
(Plating efficiency), inflammatory response (induction of IL-8 and MCP-1 by Enzyme
linked immune sorbent assay), DNA damage (strand breaks and oxidised DNA lesions by
the Comet assay) were all assessed in human lung carcinoma epithelial cells (A549),
and the mutagenic potential of ENMs (Mammalian hprt gene mutation test) was
assessed in V79-4 cells as per the OECD protocol. Detailed physico-chemical
characterization of the ENMs was performed in water and in biological media as a
prerequisite to assessment of their impacts on cells. To study the relationship
between the surface area of the ENMs and the number of ENMs with the biological
response observed, Ag ENMs concentrations were recalculated from mu g/cm(2) to ENMs
cm(2)/cm(2) and ENMs/cm(2). Results: Studied Ag ENMs are cytotoxic and cytostatic,
and induced strand breaks, DNA oxidation, inflammation and gene mutations. Results
expressed in mass unit [mu g/cm(2)] suggested that the toxicity of Ag ENMs is size
dependent with 50 nm being most toxic. However, re-calculation of Ag ENMs
concentrations from mass unit to surface area and number of ENMs per cm(2)
highlighted that 200 nm Ag ENMs, are the most toxic. Results from hprt gene
mutation assay showed that Ag ENMs 200 nm are the most mutagenic irrespective of
the concentration unit expressed. Conclusion: We found that the toxicity of Ag ENMs
is not always size dependent. Strong cytotoxic and genotoxic effects were observed
in cells exposed to Ag ENMs 50 nm, but Ag ENMs 200 nm had the most mutagenic
potential. Additionally, we showed that expression of concentrations of ENMs in
mass units is not representative. Number of ENMs or surface area of ENMs (per
cm(2)) seem more precise units with which to compare the toxicity of different
ENMs.
AN - WOS:000346748200001
AU - Huk, A.
AU - Izak-Nau, E.
AU - Reidy, B.
AU - Boyles, M.
AU - Duschl, A.
AU - Lynch, I.
AU - Dusinska, M.
C7 - 65
DA - DEC 3
DO - 10.1186/s12989-014-0065-1
PY - 2014
SN - 1743-8977
ST - Is the toxic potential of nanosilver dependent on its size?
TI - Is the toxic potential of nanosilver dependent on its size?
VL - 11
ID - 6387
ER -
TY - JOUR
AB - The engineering of vascular regeneration still involves barriers that need to
be conquered. In the current study, a novel nanocomposite comprising of fibronectin
(denoted as FN) and a small amount of silver nanoparticles (AgNP, similar to 15.1,
similar to 30.2 or similar to 75.5 ppm) was developed and its biological function
and biocompatibility in Wharton's jelly-derived mesenchymal stem cells (MSCs) and
rat models was investigated. The surface morphology as well as chemical composition
for pure FN and the FN-AgNP nanocomposites incorporating various amounts of AgNP
were firstly characterized by atomic force microscopy (AFM), UV-Visible
spectroscopy (UV-Vis), and Fourier-transform infrared spectroscopy (FTIR). Among
the nanocomposites, FN-AgNP with 30.2 ppm silver nanoparticles demonstrated the
best biocompatibility as assessed through intracellular ROS production,
proliferation of MSCs, and monocytes activation. The expression levels of pro-
inflammatory cytokines, TNF-alpha, IL-1 beta, and IL-6, were also examined. FN-AgNP
30.2 ppm significantly inhibited pro-inflammatory cytokine expression compared to
other materials, indicating superior performance of anti-immune response.
Mechanistically, FN-AgNP 30.2 ppm significantly induced greater expression of
vascular endothelial growth factor (VEGF) and stromal-cell derived factor-1 alpha
(SDF-1 alpha) and promoted the migration of MSCs through matrix metalloproteinase
(MMP) signaling pathway. Besides, in vitro and in vivo studies indicated that FN-
AgNP 30.2 ppm stimulated greater protein expressions of CD31 and von Willebrand
Factor (vWF) as well as facilitated better endothelialization capacity than other
materials. Furthermore, the histological tissue examination revealed the lowest
capsule formation and collagen deposition in rat subcutaneous implantation of FN-
AgNP 30.2 ppm. In conclusion, FN-AgNP nanocomposites may facilitate the migration
and proliferation of MSCs, induce endothelial cell differentiation, and attenuate
immune response. These finding also suggests that FN-AgNP may be a potential anti-
inflammatory surface modification strategy for vascular biomaterials.
AN - WOS:000695587400001
AU - Hung, H. S.
AU - Chang, K. B.
AU - Tang, C. M.
AU - Ku, T. R.
AU - Kung, M. L.
AU - Yu, A. Y. H.
AU - Shen, C. C.
AU - Yang, Y. C.
AU - Hsieh, H. H.
AU - Hsu, S. H.
C7 - 9262
DA - SEP
DO - 10.3390/ijms22179262
IS - 17
PY - 2021
SN - 1422-0067
ST - Anti-Inflammatory Fibronectin-AgNP for Regulation of Biological Performance
and Endothelial Differentiation Ability of Mesenchymal Stem Cells
TI - Anti-Inflammatory Fibronectin-AgNP for Regulation of Biological Performance
and Endothelial Differentiation Ability of Mesenchymal Stem Cells
VL - 22
ID - 5874
ER -
TY - JOUR
AB - The engineering of vascular regeneration still involves barriers that need to
be conquered. In the current study, a novel nanocomposite comprising of fibronectin
(denoted as FN) and a small amount of silver nanoparticles (AgNP, ~15.1, ~30.2 or
~75.5 ppm) was developed and its biological function and biocompatibility in
Wharton’s jelly‐derived mesenchymal stem cells (MSCs) and rat models was
investigated. The surface morphology as well as chemical composition for pure FN
and the FN‐AgNP nanocomposites incorporating various amounts of AgNP were firstly
characterized by atomic force microscopy (AFM), UV‐Visible spectroscopy (UV‐Vis),
and Fourier‐transform infrared spectroscopy (FTIR). Among the nanocomposites, FN‐
AgNP with 30.2 ppm silver nanoparticles demonstrated the best biocompatibility as
assessed through intracellular ROS production, proliferation of MSCs, and monocytes
activation. The expression levels of pro‐inflammatory cytokines, TNF‐α, IL‐1β, and
IL‐6, were also examined. FN‐AgNP 30.2 ppm significantly inhibited pro‐inflammatory
cytokine expression compared to other materials, indicating superior performance of
anti‐immune response. Mechanistically, FN‐AgNP 30.2 ppm significantly induced
greater expression of vascular endothelial growth factor (VEGF) and stromal‐cell
derived factor‐1 alpha (SDF‐1α) and promoted the migration of MSCs through matrix
metalloproteinase (MMP) signaling pathway. Besides, in vitro and in vivo studies
indicated that FN‐AgNP 30.2 ppm stimulated greater protein expressions of CD31 and
von Willebrand Factor (vWF) as well as facilitated better endothelialization
capacity than other materials. Furthermore, the histological tissue examination
revealed the lowest capsule formation and collagen deposition in rat subcutaneous
implantation of FN‐ AgNP 30.2 ppm. In conclusion, FN‐AgNP nanocomposites may
facilitate the migration and proliferation of MSCs, induce endothelial cell
differentiation, and attenuate immune response. These finding also suggests that
FN‐AgNP may be a potential anti‐inflammatory surface modification strategy for
vascular biomaterials. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Hung, H. S.
AU - Chang, K. B.
AU - Tang, C. M.
AU - Ku, T. R.
AU - Kung, M. L.
AU - Yu, A. Y. H.
AU - Shen, C. C.
AU - Yang, Y. C.
AU - Hsieh, H. H.
AU - Hsu, S. H.
C7 - 9262
DB - Scopus
DO - 10.3390/ijms22179262
IS - 17
KW - Endothelial differentiation
Fibronectin
Vascular tissue engineering
Animals
Cell Proliferation
Cells, Cultured
Cytoskeleton
Endothelial Cells
Fibronectins
Metal Nanoparticles
Particle Size
Rats
Silver
CD68 antigen
collagen
dimer
fibronectin
interleukin 16
interleukin 1beta
nanocomposite
silver nanoparticle
stromal cell derived factor 1alpha
vasculotropin
metal nanoparticle
silver
animal cell
animal experiment
animal model
Article
biocompatibility
cell activation
cell migration
cell proliferation
controlled study
cultural anthropology
endothelium cell
female
fibroblast
histology
immune response
in vitro study
in vivo study
macrophage
Masson staining
monocyte
nonhuman
particle size
polarization
protein expression
rat
signal transduction
surface property
animal
cell culture
cytology
cytoskeleton
drug effect
metabolism
ultrastructure
M3 - Article
PY - 2021
ST - Anti‐inflammatory fibronectin‐agnp for regulation of biological performance
and endothelial differentiation ability of mesenchymal stem cells
TI - Anti‐inflammatory fibronectin‐agnp for regulation of biological performance
and endothelial differentiation ability of mesenchymal stem cells
85113466909&doi=10.3390%2fijms22179262&partnerID=40&md5=dd025605dd1ce0a82ffd7974b88
7ea1c
VL - 22
ID - 5251
ER -
TY - JOUR
AB - A nanocomposite composed of polyethylene glycol (PEG) incorporated with
various concentrations (~17.4, ~43.5, ~174 ppm) of gold nanoparticles (Au) was
created to investigate its biocompatibility and biological performance in vitro and
in vivo. First, surface topography and chemical composition was determined through
UV-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR),
atomic force microscopy (AFM), scanning electron microscopy (SEM), free radical
scavenging ability, and water contact angle measurement. Additionally, the
diameters of the PEG-Au nanocomposites were also evaluated through dynamic light
scattering (DLS) assay. According to the results, PEG containing 43.5 ppm of Au
demonstrated superior biocompatibility and biological properties for mesenchymal
stem cells (MSCs), as well as superior osteogenic differentiation, adipocyte
differentiation, and, particularly, neuronal differentiation. Indeed, PEG-Au 43.5
ppm induced better cell adhesion, proliferation and migration in MSCs. The higher
expression of the SDF-1 alpha/CXCR4 axis may be associated with MMPs activation and
may have also promoted the differentiation capacity of MSCs. Moreover, it also
prevented MSCs from apoptosis and inhibited macrophage and platelet activation, as
well as reactive oxygen species (ROS) generation. Furthermore, the anti-
inflammatory, biocompatibility, and endothelialization capacity of PEG-Au was
measured in a rat model. After implanting the nanocomposites into rats
subcutaneously for 4 weeks, PEG-Au 43.5 ppm was able to enhance the anti-immune
response through inhibiting CD86 expression (M1 polarization), while also reducing
leukocyte infiltration (CD45). Moreover, PEG-Au 43.5 ppm facilitated CD31
expression and anti-fibrosis ability. Above all, the PEG-Au nanocomposite was
evidenced to strengthen the differentiation of MSCs into various cells, including
fat, vessel, and bone tissue and, particularly, nerve cells. This research has
elucidated that PEG combined with the appropriate amount of Au nanoparticles could
become a potential biomaterial able to cooperate with MSCs for tissue regeneration
engineering.
AN - WOS:000724519200001
AU - Hung, H. S.
AU - Kao, W. C.
AU - Shen, C. C.
AU - Chang, K. B.
AU - Tang, C. M.
AU - Yang, M. Y.
AU - Yang, Y. C.
AU - Yeh, C. A.
AU - Li, J. J.
AU - Hsieh, H. H.
C7 - 2854
DA - NOV
DO - 10.3390/cells10112854
IS - 11
PY - 2021
SN - 2073-4409
ST - Inflammatory Modulation of Polyethylene Glycol-AuNP for Regulation of the
Neural Differentiation Capacity of Mesenchymal Stem Cells
T2 - CELLS
TI - Inflammatory Modulation of Polyethylene Glycol-AuNP for Regulation of the
Neural Differentiation Capacity of Mesenchymal Stem Cells
VL - 10
ID - 6134
ER -
TY - JOUR
AB - Cardiovascular Diseases (CVDs) such as atherosclerosis, where inflammation
occurs in the blood vessel wall, are one of the major causes of death worldwide.
Mesenchymal Stem Cells (MSCs)-based treatment coupled with nanoparticles is
considered to be a potential and promising therapeutic strategy for vascular
regeneration. Thus, angiogenesis enhanced by nanoparticles is of critical concern.
In this study, Polyethylene Glycol (PEG) incorporated with 43.5 ppm of gold (Au)
nanoparticles was prepared for the evaluation of biological effects through in
vitro and in vivo assessments. The physicochemical properties of PEG and PEG-Au
nanocomposites were first characterized by UV-Vis spectrophotometry (UV-Vis),
Fourier-transform infrared spectroscopy (FTIR), and Atomic Force Microscopy (AFMs).
Furthermore, the reactive oxygen species scavenger ability as well as the
hydrophilic property of the nanocomposites were also investigated. Afterwards, the
biocompatibility and biological functions of the PEG-Au nanocomposites were
evaluated through in vitro assays. The thin coating of PEG containing 43.5 ppm of
Au nanoparticles induced the least platelet and monocyte activation. Additionally,
the cell behavior of MSCs on PEG-Au 43.5 ppm coating demonstrated better cell
proliferation, low ROS generation, and enhancement of cell migration, as well as
protein expression of the endothelialization marker CD31, which is associated with
angiogenesis capacity. Furthermore, anti-inflammatory and endothelial
differentiation ability were both evaluated through in vivo assessments. The
evidence demonstrated that PEG-Au 43.5 ppm implantation inhibited capsule formation
and facilitated the expression of CD31 in rat models. TUNEL assay also indicated
that PEG-Au nanocomposites would not induce significant cell apoptosis. The above
results elucidate that the surface modification of PEG-Au nanomaterials may enable
them to serve as efficient tools for vascular regeneration grafts.
AN - WOS:000742938000001
AU - Hung, H. S.
AU - Yang, Y. C.
AU - Kao, W. C.
AU - Yeh, C. A.
AU - Chang, K. B.
AU - Tang, C. M.
AU - Hsieh, H. H.
AU - Lee, H. T.
C7 - 4265
DA - DEC
DO - 10.3390/polym13234265
IS - 23
PY - 2021
SN - 2073-4360
ST - Evaluation of the Biocompatibility and Endothelial Differentiation Capacity
of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites
T2 - POLYMERS
TI - Evaluation of the Biocompatibility and Endothelial Differentiation Capacity
of Mesenchymal Stem Cells by Polyethylene Glycol Nanogold Composites
VL - 13
ID - 6242
ER -
TY - JOUR
AB - The indications for use of programed cell death receptor (PD-1) inhibitors to
treat cancer continues to expand rapidly. Treatment with PD-1 inhibitors has been
associated with numerous immune-mediated mucocutaneous side effects. Here, we
report 2 cases of severe mucositis caused by the PD-1 inhibitor pembrolizumab and
review the defining features of similar cases. Recognition of mucocutaneous
toxicities of PD-1 inhibitors is increasingly important as their use continues to
expand. A stepwise approach to diagnosis and management is also reviewed. © 2020
American Association of Oral and Maxillofacial Surgeons
AU - Huntley, R. E.
AU - DeNiro, K.
AU - Yousef, J.
AU - Sheedy, M.
AU - Dillon, J. K.
DB - Scopus
DO - 10.1016/j.joms.2020.11.023
IS - 6
KW - Algorithms
Antibodies, Monoclonal
Antibodies, Monoclonal, Humanized
Humans
Mucositis
Programmed Cell Death 1 Receptor
aciclovir
chlorhexidine
dexamethasone
lidocaine
methenamine
mouthwash
nystatin
pembrolizumab
prednisone
monoclonal antibody
programmed death 1 receptor
adult
advanced cancer
aged
antifungal therapy
Article
basement membrane
behavior disorder
bladder cancer
body weight loss
buccal mucosa
cachexia
cancer immunotherapy
case report
cheilitis
clinical article
comparative study
drug substitution
drug withdrawal
eating
erosion
esophagogastroduodenoscopy
esophagus biopsy
eye examination
fingernail onychomycosis
gastroenterology
graft versus host reaction
granulation tissue
histology
human
hyperplasia
hypertransaminasemia
immunofluorescence
inflammation
infusion
lip
male
medical history
metastasis
middle aged
mouth mucosa
mouth pain
mouth ulcer
mucosa inflammation
non small cell lung cancer
odynophagia
oral biopsy
oral mucositis
oropharynx
outpatient department
parakeratosis
periodic acid Schiff stain
physical examination
punch biopsy
seizure
silver staining
skin biopsy
skin injury
skin necrosis
tongue ulcer
virus culture
algorithm
M3 - Article
PY - 2021
SP - 1262-1269
ST - Severe Mucositis Secondary to Pembrolizumab: Reports of Two Cases, Review of
the Literature, and an Algorithm for Management
T2 - Journal of Oral and Maxillofacial Surgery
TI - Severe Mucositis Secondary to Pembrolizumab: Reports of Two Cases, Review of
the Literature, and an Algorithm for Management
85099665773&doi=10.1016%2fj.joms.2020.11.023&partnerID=40&md5=96f0b720dd84ff90da0b7
1bf97043fea
VL - 79
ID - 5189
ER -
TY - JOUR
AB - Owing to their tremendous potential, the inference of nano-scaled materials
has revolutionized many fields including the medicine and health, particularly for
development of various types of targeted drug delivery devices for early prognosis
and successful treatment of various diseases, including the brain disorders. Owing
to their unique characteristic features, a variety of nanomaterials (particularly,
ultra-fine particles (UFPs) have shown tremendous success in achieving the
prognostic and therapeutic goals for early prognosis and treatment of various brain
maladies such as Alzheimer's disease, Parkinson's disease, brain lymphomas, and
other ailments. However, serious attention is needful due to innumerable after-
effects of the nanomaterials. Despite their immense contribution in optimizing the
prognostic and therapeutic modalities, biological interaction of nanomaterials with
various body tissues may produce severe nanotoxicity of different organs including
the heart, liver, kidney, lungs, immune system, gastro-intestinal system, skin as
well as nervous system. However, in this review, we have primarily focused on
nanomaterials-induced neurotoxicity of the brain. Following their translocation
into different regions of the brain, nanomaterials may induce neurotoxicity through
multiple mechanisms including the oxidative stress, DNA damage, lysosomal
dysfunction, inflammatory cascade, apoptosis, genotoxicity, and ultimately necrosis
of neuronal cells. Our findings indicated that rigorous toxicological evaluations
must be carried out prior to clinical translation of nanomaterials-based
formulations to avoid serious neurotoxic complications, which may further lead to
develop various neuro-degenerative disorders.
AN - WOS:000600791600063
AU - Hussain, Z.
AU - Thu, H. E.
AU - Elsayed, I.
AU - Abourehab, M. A. S.
AU - Khan, S.
AU - Sohail, M.
AU - Sarfraz, R. M.
AU - Farooq, M. A.
DA - DEC 10
DO - 10.1016/j.jconrel.2020.10.053
PY - 2020
SN - 0168-3659
1873-4995
SP - 873-894
ST - Nano-scaled materials may induce severe neurotoxicity upon chronic exposure
to brain tissues: A critical appraisal and recent updates on predisposing factors,
underlying mechanism, and future prospects
T2 - JOURNAL OF CONTROLLED RELEASE
TI - Nano-scaled materials may induce severe neurotoxicity upon chronic exposure
to brain tissues: A critical appraisal and recent updates on predisposing factors,
underlying mechanism, and future prospects
VL - 328
ID - 6618
ER -
TY - JOUR
AB - Owing to their tunable physicochemical features and wide range of biomedical
applications, silver nanoparticles (SNPs) have been extensively investigated.
Despite exhibiting strong biomedical potential, therapeutic significance of SNPs is
limited, particularly when employed alone for treatment of chronic severe
conditions (infected diabetic wounds, cancer, etc.). These restrictions convinced
researchers to develop newer hybrid SNPs-based nanomaterials to fully replicate
characteristics of ideal nanomaterials. Therefore, the present review was aimed to
reconceptualise emerging adaptations such as biofabrication, functionalization, and
hybridization of SNPs with biological macromolecules in the form of nanocomposites,
sponges, hydrogel, scaffolds, electrospun nanofibers, and nanomembrane for
improvement of their biomedical implications. Functionalization of SNPs with
biological macromolecules showed promising improvement of their various biomedical
properties such as wound healing, tissue regeneration, antibacterial, antifungal,
anticancer, anti-inflammatory, antioxidant, and anti-diabetic. Similarly,
hybridization of SNPs with one or more biopolymers (chitosan, hyaluronic acid (HA),
cellulose, alginate, starch, etc.) has further improved their physicochemical
characteristics (morphology, tensile strength, and release kinetics),
biocompatibility, pharmacokinetic profile, and synergistic biomedical efficacy.
Recent developments and progress made in the designing of SNPs-based hybrid
nanomaterials, their superior biomedical efficacies, current challenges to their
clinical transition and future prospects, have also been reconceptualised. © 2019
Elsevier B.V.
AU - Hussain, Z.
AU - Thu, H. E.
AU - Sohail, M.
AU - Khan, S.
C7 - 101169
DB - Scopus
DO - 10.1016/j.jddst.2019.101169
KW - Biological macromolecules
Functionalization
Hybridization
Upgraded biomedical efficacy
biopolymer
silver nanoparticle
drug efficacy
human
hybridization
hydrogel
in vitro study
macromolecule
nonhuman
Review
tissue regeneration
wound healing
M3 - Review
PY - 2019
ST - Hybridization and functionalization with biological macromolecules
synergistically improve biomedical efficacy of silver nanoparticles:
Reconceptualization of in-vitro, in-vivo and clinical studies
T2 - Journal of Drug Delivery Science and Technology
TI - Hybridization and functionalization with biological macromolecules
synergistically improve biomedical efficacy of silver nanoparticles:
Reconceptualization of in-vitro, in-vivo and clinical studies
85073098739&doi=10.1016%2fj.jddst.2019.101169&partnerID=40&md5=e3623359b9610d934cf5
3335f8a078f2
VL - 54
ID - 5433
ER -
TY - CHAP
AB - Nanoparticles (NPs) can be developed to improve drug penetration and reorient
chemotherapy, or selectively target the cancer cells or cell compartment. Both
passive and active targeting strategies are used to redirect the anticancer drugs.
Noble metals such as the silver NPs (AgNPs) are characterized by electrical,
optical, and thermal properties, and can be integrated into products for optical,
biological and chemical sensor applications such as pastes, conductive inks, and
fillers for high stabilization, electrical conductivity, and low sintering
temperatures. The biosynthesis of AgNPs, making use of bacteria, fungi,
actinomycetes, yeast, algae, and plants, is eco-friendly, green, nontoxic and
inexpensive. The AgNPs sytnhesized are of various shapes and sizes. The AgNPs
have diverse bioactivities including antibacterial, antifungal, antiviral, anti-
inflammatory, anti-angiogenic, and anticancer activities, with great potential for
use in cancer diagnosis and therapy. The mechanisms of AgNP-induced cytotoxicity
include endoplasmic reticulum stress, lactate dehydrogenase leakage, and enhanced
reactive oxygen species level. Co-application of AgNPs and natural products could
play an essential role in nanoscience and nanotechnology, especially in
nanomedicine for cancer diagnosis and therapeutics. © 2020, Springer Nature
Switzerland AG.
AU - Hussein, H. A.
AU - Abdullah, M. A.
DB - Scopus
DO - 10.1007/978-3-030-41464-1_14
KW - Anti-cancer
Nanobiotechnology
Nanocarrier
Nanomedicine
PY - 2020
SP - 313-332
ST - Biosynthesis, Mechanisms, and Biomedical Applications of Silver Nanoparticles
T2 - Nanotechnology in the Life Sciences
TI - Biosynthesis, Mechanisms, and Biomedical Applications of Silver Nanoparticles
85102114308&doi=10.1007%2f978-3-030-41464-
1_14&partnerID=40&md5=01acf1b4e976fcd7fffbe6c29a279709
ID - 5311
ER -
TY - JOUR
AB - Here we developed a novel green synthesis method for gold nanoparticles (CGA-
AuNPs) using chlorogenic acid (CGA) as reductants without the use of other
chemicals and validated the anti-inflammatory efficacy of CGA-AuNPs in vitro and in
vivo. The resulting CGA-AuNPs appeared predominantly spherical in shape with an
average diameter of 22.25 +/- 4.78 nm. The crystalline nature of the CGA-AuNPs was
confirmed by high-resolution X-ray diffraction and by selected-area electron
diffraction analyses. High-resolution liquid chromatography/electrospray ionization
mass spectrometry revealed that the caffeic acid moiety of CGA forms quinone
structure through a two-electron oxidation causing the reduction of Au3+ to Au-0.
When compared to CGA, CGA-AuNPs exhibited enhanced anti-inflammatory effects on NF-
kappa B-mediated inflammatory network, as well as cell adhesion. Collectively,
green synthesis of CGA-AuNPs using bioactive reductants and mechanistic studies
based on mass spectrometry may open up new directions in nanomedicine and CGA-AuNPs
can be an anti-inflammatory nanomedicine for future applications. From the Clinical
Editor: Gold nanoparticles (Au NPs) have been shown to be very useful in many
applications due to their easy functionalization capability. In this article, the
authors demonstrated a novel method for the synthesis of gold nanoparticles using
chlorogenic acid (CGA) as reductants. In-vitro experiments also confirmed
biological activity of the resultant gold nanoparticles. Further in-vivo studies
are awaited. (C) 2015 Elsevier Inc. All rights reserved.
AN - WOS:000365594700008
AU - Hwang, S. J.
AU - Jun, S. H.
AU - Park, Y.
AU - Cha, S. H.
AU - Yoon, M.
AU - Cho, S.
AU - Lee, H. J.
DA - OCT
DO - 10.1016/j.nano.2015.05.002
IS - 7
PY - 2015
SN - 1549-9634
1549-9642
SP - 1677-1688
ST - Green synthesis of gold nanoparticles using chlorogenic acid and their
enhanced performance for inflammation
T2 - NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
TI - Green synthesis of gold nanoparticles using chlorogenic acid and their
enhanced performance for inflammation
VL - 11
ID - 6571
ER -
TY - JOUR
AB - Background: Burns are considered a critical care problem in emergency
medicine, resulting in physical, psychological, and chronic disabilities. Silver
sulfadiazine is the gold standard in topical burn treatment but was associated with
toxicity to keratinocytes and fibroblasts, which may delay wound healing. In
discovering potential alternative treatments for burn wound healing, this study was
performed to determine the effect of Labisia Pumila (Blume) Fern.-Vill. Var. Alata
(LPVa) extract on thermal-burn wounds in rats. Methods: A total of 50 Sprague-
Dawley male rats were categorized into five groups. There were three control
groups; normal control (left untreated), negative control (given ointment base) and
positive control (given silver sulfadiazine). Meanwhile, the two intervention
groups were given with 2% LPVa leaf and root extracts, respectively. Burn wounds
were inflicted on the loin region of the rat by applying a heated steel rod at 80°C
for 10 s. On days 3, 7, 14, and 21, wounds were measured macroscopically using a
digital calliper and one animals of each group were sacrificed, and the wounded
skin were excised for histomorphological assessments. The wounds were excised for
hydroxyproline content on Day 14 of treatment. Result: For wound contraction
percentage, both the leaf and root extracts of LPVa showed a significant reduction
in burn wound size on Day 7 onwards, when compared to other groups. For
hydroxyproline content, only the leaf extract of LPVa produced significantly higher
content compared to both negative and normal control groups. In terms of
histological examination, the leaf extract group demonstrated a superior healing
effect than the root extract group. Conclusion: Both leaf and root extracts of LPVa
could promote wound healing in the thermal-burn wound rat model, with leaf extract
being superior to root extract. Copyright © 2022 Ibrahim, Mohamed, Mohamed, Mohd
Ramli and Shuid.
AU - Ibrahim, N. I.
AU - Mohamed, I. N.
AU - Mohamed, N.
AU - Mohd Ramli, E. S.
AU - Shuid, A. N.
C7 - 968664
DB - Scopus
DO - 10.3389/fphar.2022.968664
KW - antioxidant
burn wound healing
histomorphology
hydroxyproline
labisia pumila
labisia pumila extract
plant extract
sulfadiazine silver
unclassified drug
animal experiment
animal model
animal tissue
area under the curve
Article
burn model
cell infiltration
controlled study
data analysis
gold standard
histology
infant
inflammatory cell
male
model
morphology
nonhuman
photography
rat
thickness
toxicity
wound contraction
wound healing
M3 - Article
PY - 2022
ST - The effects of aqueous extract of Labisia Pumila (Blume) Fern.-Vill. Var.
Alata on wound contraction, hydroxyproline content and histological assessments in
superficial partial thickness of second-degree burn model
T2 - Frontiers in Pharmacology
TI - The effects of aqueous extract of Labisia Pumila (Blume) Fern.-Vill. Var.
Alata on wound contraction, hydroxyproline content and histological assessments in
superficial partial thickness of second-degree burn model
85140587506&doi=10.3389%2ffphar.2022.968664&partnerID=40&md5=d649d3958ec403bd1cf7a8
853e73823b
VL - 13
ID - 5099
ER -
TY - JOUR
AB - Bryonia alba L. is the only Bryonia species found in Romanian flora, being
known as a remedy for inflammatory pathologies or for its hepatoprotective and
adaptogen activities. The present investigation studied the flavonoid composition
and antioxidant activities of the aerial parts of this species. Flavonoid profile
was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant
capacity was assessed by various methods, testing di erent antioxidant mechanisms:
DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant
capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent
antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC
(silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human
cancerous and healthy cell lines. Anti-plasmodial tests were performed on two
strains of Plasmodium falciparum. Whole organism toxicity was assessed on zebrafish
larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin,
isoorientin, and isovitexin as the major flavonoids in the composition of tested
samples. Significant results were obtained for all antioxidant capacity assays. The
cytotoxicity tests proved the absence of cellular and parasitic toxicity and these
results were confirmed by the lack of toxicity on the zebrafish larvae model. This
study proves a promising potential of the aerial parts of Bryonia alba L. as
antioxidant agents. © 2019 by the authors.
AU - Ielciu, I.
AU - Frédérich, M.
AU - Hanganu, D.
AU - Angenot, L.
AU - Olah, N. K.
AU - Ledoux, A.
AU - Crisan, G.
AU - Paltinean, R.
C7 - 108
DB - Scopus
DO - 10.3390/antiox8040108
IS - 4
KW - Antioxidant
Flavonoids
HPLC-DAD
Toxicity
M3 - Article
PY - 2019
ST - Flavonoid analysis and antioxidant activities of the bryonia alba L. Aerial
parts
T2 - Antioxidants
TI - Flavonoid analysis and antioxidant activities of the bryonia alba L. Aerial
parts
85070662957&doi=10.3390%2fantiox8040108&partnerID=40&md5=caf2ea1184b7f57887e9f6742c
3e51ec
VL - 8
ID - 5393
ER -
TY - JOUR
AB - Bryonia alba L. is the only Bryonia species found in Romanian flora, being
known as a remedy for inflammatory pathologies or for its hepatoprotective and
adaptogen activities. The present investigation studied the flavonoid composition
and antioxidant activities of the aerial parts of this species. Flavonoid profile
was evaluated by HPLC coupled with Diode Array Detection (DAD), while antioxidant
capacity was assessed by various methods, testing different antioxidant mechanisms:
DPPH (2,2-diphenyl-1-picrylhydrazyl), CUPRAC (cupric reducing antioxidant
capacity), FRAP (ferric reducing ability of plasma), TEAC (Trolox equivalent
antioxidant capacity), EPR (electron paramagnetic resonance method) and SNPAC
(silver nanoparticles antioxidant capacity). Cytotoxicity was tested on human
cancerous and healthy cell lines. Anti-plasmodial tests were performed on two
strains of Plasmodium falciparum. Whole organism toxicity was assessed on zebrafish
larvae. The HPLC-DAD analysis proved the presence of lutonarin, saponarin,
isoorientin, and isovitexin as the major flavonoids in the composition of tested
samples. Significant results were obtained for all antioxidant capacity assays. The
cytotoxicity tests proved the absence of cellular and parasitic toxicity and these
results were confirmed by the lack of toxicity on the zebrafish larvae model. This
study proves a promising potential of the aerial parts of Bryonia alba L. as
antioxidant agents.
AN - WOS:000467294900032
AU - Ielciu, I.
AU - Frederich, M.
AU - Hanganu, D.
AU - Angenot, L.
AU - Olah, N. K.
AU - Ledoux, A.
AU - Crisan, G.
AU - Paltinean, R.
C7 - 108
DA - APR
DO - 10.3390/antiox8040108
IS - 4
PY - 2019
SN - 2076-3921
ST - Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial
Parts
T2 - ANTIOXIDANTS
TI - Flavonoid Analysis and Antioxidant Activities of the Bryonia alba L. Aerial
Parts
VL - 8
ID - 6344
ER -
TY - JOUR
AB - In order to investigate biocompatibility of pure metals, which are widely
used, the gingival tissues were brought into contact with pure metals. Nine kinds
of pure metals, such as gold, platinum, silver, palladium, copper, nickel, zinc,
indium and tin were used in the analysis of biocompatibility. With a No. 1/2 round
bar, a standardized small cavity was prepared in the mesiolingual cervical portion
of the upper first molar. Nine kinds of pure metals powder were inserted into the
small cavity of the maxillary root surface in rats. Post operative changes of the
gingival tissue were studied histopathologically. The results were as follows: 1.
Gold, palladium and indium powder were observed in the lamina propria of the
gingiva. Infiltration of cells was not observed around these pure metal powders.
Gold, palladium and indium powders showed little cytotoxicity. 2. Zinc powders were
not associated with chronic inflammatory charges. Zinc powders were surrounded by
an abundance of connective tissue substances. The fragments were surrounded by
collagen fibers, but no inflammatory cells were present. Zinc powders showed little
cytotoxicity. 3. Platinum and tin powders were not associated with chronic
inflammatory changes. These pure metal powders were surrounded by macrophages, but
no other mononuclear inflammatory cells were present. 4. Clumps and granules of
silver powder were not associated with chronic inflammatory changes. Multinucleate
giant cells were shown surrounding silver powders. Silver powders were observed to
have been taken into the multinucleate giant cells. Many of these cells also
contained fine dark granules in their cytoplasm, and a few contained small pieces
of silver. The tissue reaction to silver showed little cytotoxicity. 5. Copper and
nickel powders were associated with chronic inflammatory cells. Moderate chronic
inflammation and occasional neutrophils leucocytes and lymphocytes were shown to be
associated with these pure metal powders. Copper and nickel caused extensive damage
to the gingival tissues.
AU - Iijima, S.
DB - Scopus
DO - 10.2329/perio.31.997
IS - 4
KW - Animals
Copper
Gingiva
Gold
Indium
Metals
Nickel
Platinum
Rats
Silver
Tin
Zinc
copper
gold
indium
metal
nickel
platinum
silver
tin
zinc
animal
article
drug effect
gingiva
rat
M3 - Article
PY - 1989
SP - 997-1020
ST - Histopathological study of the effect of pure metals to the periodontal
tissues
T2 - Nippon Shishubyo Gakkai kaishi
TI - Histopathological study of the effect of pure metals to the periodontal
tissues
0024893887&doi=10.2329%2fperio.31.997&partnerID=40&md5=e518af03cccaaf0c2f4972ecb5a7
8f0e
VL - 31
ID - 5774
ER -
TY - JOUR
AB - Increasing use of nano-enabled products provides many benefits in various
industrial processes and medical applications, but it also raises concern about
release of nanoparticles (NPs) into the environment and subsequent human exposure.
While potential toxicity of individual NPs types has been well described in
scientific literature, exposure and health-related effects of nanomixtures has been
poorly described. This study aimed to evaluate the combined effect of silver (AgNP)
and polystyrene NPs (PSNP) on the human macrophages. AgNP are one of the most
commercialized NPs due to efficient antimicrobial activity, while PSNP are
ubiquitous in terrestrial and aquatic environments due to plastic pollution and
degradation of polystyrene-based products. Differentiated monocytic cell line THP-1
were used as an in vitro model of human macrophages. Multiple aspects of cellular
response to AgNP-PSNP nanomixture were analyzed including cell death, induction of
apoptosis, oxidative stress response, expression of pro- and anti-inflammatory
cytokines, and nanomechanical properties of cells. NPs uptake was visualized by
confocal microscopy and quantified using flow cytometry. Results show that
nanomixture increased apoptosis and cell death, expression of IL-6, IL-8 and TNFa,
oxidative stress and mitochondrial dysfunction in cells compared to AgNP and PSNP
applied as single treatments, indicating mixture additive action. Anti-inflammatory
cytokines IL1b, IL-4 and IL-10 were not affected by combined exposure compared to
single NPs. Visualization of NPs uptake and internalization showed that AgNP and
PSNP were localized mostly in cytoplasm, with small fraction of AgNP translocated
into cell nuclei, which explain increased number of double-stranded DNA breaks
following exposure of cells to AgNPs alone or in the mixture. Study outcomes
represent clear warnings on the human co-exposure to AgNP and PSNP that needs to be
implemented in risk assessment approaches towards toxic-free environment. © 2022
Elsevier B.V.
AU - Ilić, K.
AU - Kalčec, N.
AU - Krce, L.
AU - Aviani, I.
AU - Turčić, P.
AU - Pavičić, I.
AU - Vinković Vrček, I.
C7 - 110225
DB - Scopus
DO - 10.1016/j.cbi.2022.110225
KW - Apoptosis
Cytokines
Immunotoxicity
Mixture effect
Nanomechanics
Humans
Macrophages
Metal Nanoparticles
Polystyrenes
Silver
interleukin 10
interleukin 1beta
interleukin 4
interleukin 6
interleukin 8
nanoparticle
polystyrene nanoparticle
silver nanoparticle
unclassified drug
metal nanoparticle
polystyrene derivative
silver
apoptosis
aquatic environment
Article
cell death
cell function
cell nucleus
comparative study
confocal microscopy
controlled study
cytoplasm
cytotoxicity
degradation
double stranded DNA break
flow cytometry
human
human cell
in vitro study
internalization (cell)
macrophage
mitochondrial dynamics
molecular mechanics
nanotoxicology
oxidative stress
plastic pollution
protein expression
risk assessment
terrestrial surface waters
THP-1 cell line
M3 - Article
PY - 2022
ST - Toxicity of nanomixtures to human macrophages: Joint action of silver and
polystyrene nanoparticles
TI - Toxicity of nanomixtures to human macrophages: Joint action of silver and
85140451403&doi=10.1016%2fj.cbi.2022.110225&partnerID=40&md5=0133c3ed4a621ce25db359
a40058a8f7
VL - 368
ID - 4967
ER -
TY - JOUR
AB - Increasing use of nano-enabled products provides many benefits in various
industrial processes and medical applications, but it also raises concern about
release of nanoparticles (NPs) into the environment and subsequent human exposure.
While potential toxicity of individual NPs types has been well described in
scientific literature, exposure and health-related effects of nanomixtures has been
poorly described. This study aimed to evaluate the combined effect of silver (AgNP)
and polystyrene NPs (PSNP) on the human macrophages. AgNP are one of the most
commercialized NPs due to efficient antimicrobial activity, while PSNP are
ubiquitous in terrestrial and aquatic environments due to plastic pollution and
degradation of polystyrene-based products. Differentiated monocytic cell line THP-1
were used as an in vitro model of human macrophages. Multiple aspects of cellular
response to AgNP-PSNP nanomixture were analyzed including cell death, induction of
apoptosis, oxidative stress response, expression of pro-and anti-inflammatory
cytokines, and nanomechanical properties of cells. NPs up-take was visualized by
confocal microscopy and quantified using flow cytometry. Results show that
nanomixture increased apoptosis and cell death, expression of IL-6, IL-8 and TNFa,
oxidative stress and mitochondrial dysfunction in cells compared to AgNP and PSNP
applied as single treatments, indicating mixture additive action. Anti-inflammatory
cytokines IL1b, IL-4 and IL-10 were not affected by combined exposure compared to
single NPs. Visualization of NPs uptake and internalization showed that AgNP and
PSNP were localized mostly in cytoplasm, with small fraction of AgNP translocated
into cell nuclei, which explain increased number of double -stranded DNA breaks
following exposure of cells to AgNPs alone or in the mixture. Study outcomes
represent clear warnings on the human co-exposure to AgNP and PSNP that needs to be
implemented in risk assessment approaches towards toxic-free environment.
AN - WOS:000896919900005
AU - Ilic, K.
AU - Kalcec, N.
AU - Krce, L.
AU - Aviani, I.
AU - Turcic, P.
AU - Pavicic, I.
AU - Vrcek, I. V.
C6 - OCT 2022
C7 - 110225
DA - DEC 1
DO - 10.1016/j.cbi.2022.110225
PY - 2022
SN - 0009-2797
1872-7786
ST - Toxicity of nanomixtures to human macrophages: Joint action of silver and
T2 - CHEMICO-BIOLOGICAL INTERACTIONS
TI - Toxicity of nanomixtures to human macrophages: Joint action of silver and
VL - 368
ID - 6152
ER -
TY - JOUR
AB - Background non aims of the study: Prosthetic valve endocarditis (PVE) is an
infrequent but serious complication of cardiac valve replacement. PVE is a foreign
body infection predominantly based in the sewing cuff of a prosthetic heart valve
leading to thromboembolism, ring abscess, paravalvular leakage, and eventual
invasion of the myocardium. Mortality rates as high as 75% have been reported. A
silver-coated sewing cuff is now available (St. Jude Medical(R) mechanical heart
valve SJMR Masters Series with Silzone((TM)) coating) intended to inhibit the
colonization and attachment to the sewing cuff of those microorganisms commonly
associated with PVE. Silzone is a dense layer of metallic silver deposited on
individual fiber surfaces of the valve cuff. Previously, Silzone coating was shown
in vitro to decrease attachment and colonization of microorganisms with no adverse
affect on biocompatibility. The present study was designed to assess the efficacy
of Silzone-coated polyester fabric in vivo in a direct-contamination model. The
organism chosen was a pathogenic strain of Staphylococcus epidermidis capable of
producing biofilm. Methods: Infection resistance of uncoated polyester and Silzone-
coated polyester fabric was assessed by the acute inflammatory response in a guinea
pig subdermal model. Fabric samples were implanted sterile or inoculated with S.
epidermidis. The ability of the strain to produce biofilm was verified in vitro.
Samples were explanted at one and two days postoperatively. Verification of the
infecting bacteria was by colony morphology and Gram-staining properties of
bacteria from the explanted samples. Inflammation was assessed histopathologically.
Percent necrotic tissue within the fabric was determined by computer-assisted image
analysis. Results: Histopathology and image analysis of necrotic tissue showed
significantly less inflammation within the Silzone-coated fabric than within
uncoated polyester fabric. Conclusions: The Silzone coating reduced inflammation in
this direct-contamination model using a strain of S. epidermidis that is capable of
producing biofilm. This indicates a concentration of silver ions sufficient for
bacteriostatic or bactericidal activity within the fabric in vivo.
AN - WOS:000075774500010
AU - Illingworth, B. L.
AU - Tweden, K.
AU - Schroeder, R. E.
AU - Cameron, J. D.
DA - SEP
IS - 5
PY - 1998
SN - 0966-8519
SP - 524-530
ST - In vivo efficacy of silver-coated (Silzone (TM)) infection-resistant
polyester fabric against a biofilm-producing bacteria, Staphylococcus epidermidis
T2 - JOURNAL OF HEART VALVE DISEASE
TI - In vivo efficacy of silver-coated (Silzone (TM)) infection-resistant
polyester fabric against a biofilm-producing bacteria, Staphylococcus epidermidis
VL - 7
ID - 6522
ER -
TY - JOUR
AB - Background and aims of the study: Prosthetic valve endocarditis (PVE) is an
infrequent but serious complication of cardiac valve replacement. PVE is a foreign
body infection predominantly based in the sewing cuff of a prosthetic heart valve
leading to thromboembolism, ring abscess, paravalvular leakage, and eventual
invasion of the myocardium. Mortality rates as high as 75% have been reported. A
silver-coated sewing cuff is now available (St. Jude Medical® mechanical heart
valve SJMR Masters Series with Silzone(TM) coating) intended to inhibit the
colonization and attachment to the sewing cuff of those microorganisms commonly
associated with PVE. Silzone is a dense layer of metallic silver deposited on
individual fiber surfaces of the valve cuff. Previously, Silzone coating was shown
in vitro to decrease attachment and colonization of microorganisms with no adverse
affect on biocompatibility. The present study was designed to assess the efficacy
of Silzone-coated polyester fabric in vivo in a direct-contamination model. The
organism chosen was a pathogenic strain of Staphylococcus epidermidis capable of
producing biofilm. Methods: Infection resistance of uncoated polyester and Silzone-
coated polyester fabric was assessed by the acute inflammatory response in a guinea
pig subdermal model. Fabric samples were implanted sterile or inoculated with S.
epidermidis. The ability of the strain to produce biofilm was verified in vitro.
Samples were explanted at one and two days postoperatively. Verification of the
infecting bacteria was by colony morphology and Gram-staining properties of
bacteria from the explanted samples. Inflammation was assessed histopathologically.
Percent necrotic tissue within the fabric was determined by computer-assisted image
analysis. Results: Histopathology and image analysis of necrotic tissue showed
significantly less inflammation within the Silzone-coated fabric than within
uncoated polyester fabric. Conclusions: The Silzone coating reduced inflammation in
this direct-contamination model using a strain of S. epidermidis that is capable of
producing biofilm. This indicates a concentration of silver ions sufficient for
bacteriostatic or bactericidal activity within the fabric in vivo.
AU - Illingworth, B. L.
AU - Tweden, K.
AU - Schroeder, R. F.
AU - Cameron, J. D.
DB - Scopus
IS - 5
KW - Animals
Biofilms
Female
Guinea Pigs
Heart Valve Prosthesis
Male
Materials Testing
Polyesters
Polyethylene Terephthalates
Prosthesis Design
Prosthesis-Related Infections
Reference Values
Silver
polyester
silver
animal experiment
article
bacterium adherence
biofilm
controlled study
endocarditis
guinea pig
heart valve prosthesis
heart valve replacement
histopathology
infection resistance
nonhuman
priority journal
prosthesis material
staphylococcus epidermidis
thromboembolism
M3 - Article
PY - 1998
SP - 524-530
ST - In vivo efficacy of silver-coated (Silzone(TM)) infection-resistant polyester
fabric against a biofilm-producing bacteria, Staphylococcus epidermidis
T2 - Journal of Heart Valve Disease
TI - In vivo efficacy of silver-coated (Silzone(TM)) infection-resistant polyester
fabric against a biofilm-producing bacteria, Staphylococcus epidermidis
0031692053&partnerID=40&md5=bda5ad9a1c4007d6051a1021a25e556d
VL - 7
ID - 5777
ER -
TY - JOUR
AB - As a chronic inflammatory disease, atherosclerosis is responsible for
thousands of deaths worldwide each year, and it imposes massive economic costs on
individuals and on society. Because of its high importance, the discovery of
sensitive and accurate strategies for imaging, targeted drug delivery, and
therapeutic monitoring of this condition is essential. In recent years, continuous
research has achieved remarkable successes in the use of nanotechnology in the
molecular imaging and treatment of atherosclerosis. Among various nanoparticles -
such as metallic, polymeric, and lipid - metallic nanoparticles are being
considered due to their unique properties for use in treatment and imaging. It
should be taken into consideration that some of the metal nanoparticles themselves
can cause adverse biological effects, and these effects should be considered
important risk factors in the pathological pathways leading up to atherosclerosis.
This review provides a description of the applications and potential toxicity of
metal nanoparticles in atherosclerosis. © 2015 Fatemeh Imanparast, Mahmood Doosti
and Mohammad Ali Faramarzi.
AU - Imanparast, F.
AU - Doosti, M.
AU - Faramarzi, M. A.
DB - Scopus
DO - 10.5101/nbe.v7i3.p111-127
IS - 3
KW - Atherosclerosis
Drug delivery
Imaging
Metals
Nanoparticles
Toxicity
colony stimulating factor
gamma interferon
gold nanoparticle
metal nanoparticle
nickel nanoparticle
oxidized low density lipoprotein
PADGEM protein
platelet derived growth factor
silver nanoparticle
superparamagnetic iron oxide nanoparticle
antiangiogenic activity
atherogenesis
atherosclerosis
atherosclerotic plaque
blood clotting
blood vessel rupture
cell migration
cell proliferation
disease association
endothelial dysfunction
endothelium cell
endothelium injury
foam cell
inflammation
macrophage
molecular imaging
molecular pathology
monocyte
nanomedicine
nanotoxicology
neutrophil
Review
tight junction
vascular smooth muscle cell
M3 - Review
PY - 2015
SP - 111-127
ST - Metal nanoparticles in atherosclerosis: Applications and potential toxicity
T2 - Nano Biomedicine and Engineering
TI - Metal nanoparticles in atherosclerosis: Applications and potential toxicity
84945194856&doi=10.5101%2fnbe.v7i3.p111-
127&partnerID=40&md5=bdb949ff78f5994cd754aeac26fe907e
VL - 7
ID - 5613
ER -
TY - JOUR
AB - Background: Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological
agents widely used in oral medicine and dental practice due to their anti-
microbial/anti-cavity properties. However, risks associated with the co-exposure of
local cells and tissues to these xenobiotics are not clear. Therefore, we have
evaluated the effects of AgNPs and F co-exposure on human gingival fibroblast
cells. Methods: Human gingival fibroblast cells (CRL-2014) were exposed to AgNPs
and/or F at different concentrations for up to 24 hours. Cellular uptake of AgNPs
was examined by transmission electron microscopy. Downstream inflammatory effects
and oxidative stress were measured by real-time quantitative polymerase chain
reaction (PCR) and reactive oxygen species (ROS) generation. Cytotoxicity and
apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay and real-time quantitative PCR and flow cytometry,
respectively. Finally, the involvement of mitogen-activated protein kinases (MAPK)
was studied using Western blot. Results: We found that AgNPs penetrated the cell
membrane and localized inside the mitochondria. Co-incubation experiments resulted
in increased oxidative stress, inflammation, and apoptosis. In addition, we found
that co-exposure to both xenobiotics phosphorylated MAPK, particularly p42/44 MAPK.
Conclusion: A combined exposure of human fibroblasts to AgNPs and F results in
increased cellular damage. Further studies are needed in order to evaluate
pharmacological and potentially toxicological effects of AgNPs and F on oral
health. © 2014 Inkielewicz-Stepniak et al.
AU - Inkielewicz-Stepniak, I.
AU - Santos-Martinez, M. J.
AU - Medina, C.
AU - Radomski, M. W.
DB - Scopus
DO - 10.2147/IJN.S59172
IS - 1
KW - Apoptosis
Inflammation
Matrix
Metalloproteinases
Mitogen-activated protein kinases
Nanoparticles
Oxidative stress
Cell Line
Drug Combinations
Fibroblasts
Gingiva
Humans
Metal Nanoparticles
Silver
Sodium Fluoride
apoptosis
inflammation
matrix
metalloproteinases
mitogen-activated protein kinases
nanoparticles
oxidative stress
silver nanoparticle
sodium fluoride
article
cell membrane
cell viability
controlled study
cytotoxicity
fibroblast
gingiva
human
human cell
lipid peroxidation
mitochondrion
M3 - Article
PY - 2014
SP - 1677-1687
ST - Pharmacological and toxicological effects of co-exposure of human gingival
fibroblasts to silver nanoparticles and sodium fluoride
TI - Pharmacological and toxicological effects of co-exposure of human gingival
fibroblasts to silver nanoparticles and sodium fluoride
84897949561&doi=10.2147%2fIJN.S59172&partnerID=40&md5=457d9f5ac656743a9cc916812109a
b5c
VL - 9
ID - 5605
ER -
TY - JOUR
AB - Chronic inflammation intensifies the risk for malignant neoplasm, indicating
that curbing inflammation could be a valid strategy to prevent or cure cancer.
Cancer and inflammation are inter-related diseases and many anti-inflammatory
agents are also used in chemotherapy. Earlier, we have reported a series of novel
ligands and respective binuclear Ag(I)-NHC complexes (NHC = N-heterocyclic carbene)
with potential anticancer activity. In the present study, a newly synthesized salt
(II) and respective Ag(I)-NHC complex (HI) of comparable molecular framework were
prepared for a further detailed study. Preliminarily, II and III were screened
against HCT-116 and PC-3 cells, wherein III showed better results than II. Both the
compounds showed negligible toxicity against normal CCD-18Co cells. In FAM-FLICA
caspase assay, III remarkably induced caspase-3/7 in HCT-116 cells most probably by
tumor necrosis factor-alpha (TNF-alpha) independent intrinsic pathway and
significantly inhibited in vitro synthesis of cytokines, interleukin-1 (IL-1) and
TNF-alpha in human macrophages (U937 cells). In a cell-free system, both the
compounds inhibited cyclooxygenase (COX) activities, with III being more selective
towards COX-2. The results revealed that HI has strong antiproliferative property
selectively against colorectal tumor cells which could be attributed to its pro-
apoptotic and anti-inflammatory abilities. (C) 2015 Published by Elsevier Inc.
AN - WOS:000353252600001
AU - Iqbal, M. A.
AU - Umar, M. I.
AU - Haque, R. A.
AU - Ahamed, M. B. K.
AU - Bin Asmawi, M. Z.
AU - Majid, Amsa
DA - MAY
DO - 10.1016/j.jinorgbio.2015.02.001
PY - 2015
SN - 0162-0134
1873-3344
SP - 1-13
ST - Macrophage and colon tumor cells as targets for a binuclear silver(I) N-
heterocyclic carbene complex, an anti-inflammatory and apoptosis mediator
T2 - JOURNAL OF INORGANIC BIOCHEMISTRY
TI - Macrophage and colon tumor cells as targets for a binuclear silver(I) N-
VL - 146
ID - 5921
ER -
TY - JOUR
AB - Chronic inflammation intensifies the risk for malignant neoplasm, indicating
that curbing inflammation could be a valid strategy to prevent or cure cancer.
Cancer and inflammation are inter-related diseases and many antiinflammatory agents
are also used in chemotherapy. Earlier, we have reported a series of novel ligands
and respective binuclear Ag(I)-NHC complexes (NHC = N-heterocyclic carbene) with
potential anticancer activity. In the present study, a newly synthesized salt (II)
and respective Ag(I)-NHC complex (III) of comparable molecular framework were
prepared for a further detailed study. Preliminarily, II and III were screened
against HCT-116 and PC-3 cells, wherein III showed better results than II. Both the
compounds showed negligible toxicity against normal CCD-18Co cells. In FAM-FLICA
caspase assay, III remarkably induced caspase-3/7 in HCT-116 cells most probably by
tumor necrosis factor-alpha (TNF-α) independent intrinsic pathway and significantly
inhibited in vitro synthesis of cytokines, interleukin-1 (IL-1) and TNF-α in human
macrophages (U937 cells). In a cell-free system, both the compounds inhibited
cyclooxygenase (COX) activities, with III being more selective towards COX-2. The
results revealed that III has strong antiproliferative property selectively against
colorectal tumor cells which could be attributed to its pro-apoptotic and anti-
inflammatory abilities. © 2015, Elsevier Inc. All rights reserved.
AU - Iqbal, M. A.
AU - Umar, M. I.
AU - Haque, R. A.
AU - Khadeer Ahamed, M. B.
AU - Asmawi, M. Z. B.
AU - Majid, A. M. S. A.
DB - Scopus
DO - 10.1016/j.jinorgbio.2015.02.001
KW - Anti-cancer
Anti-inflammatory
Benzimidazole
Benzimidazolium salts
N-heterocyclic carbenes (NHCs)
Silver-NHC complexes
Apoptosis
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
HCT116 Cells
Humans
Interleukin-1
Macrophages
Organometallic Compounds
U937 Cells
carbene
cyclooxygenase 2 inhibitor
interleukin 1
prostaglandin synthase
silver
cyclooxygenase 2
organometallic compound
apoptosis
Article
caspase assay
cell free system
chemical structure
colon tumor
colorectal tumor
macrophage
synthesis
drug effects
genetics
HCT116 cell line
human
metabolism
U937 cell line
M3 - Article
PY - 2015
SP - 1-13
ST - Macrophage and colon tumor cells as targets for a binuclear silver(I) N-
TI - Macrophage and colon tumor cells as targets for a binuclear silver(I) N-
84922940948&doi=10.1016%2fj.jinorgbio.2015.02.001&partnerID=40&md5=245e30bf47d73839
bf45452d4d38bd39
VL - 146
ID - 5630
ER -
TY - JOUR
AB - Partial-thickness burn injury has the potential for reepithelialization and
heals within 3 weeks. If the wound is infected by bacteria before reepithelization,
however, the depth of disruption increases and the lesion easily progresses to the
full-thickness dermal layers. In the treatment of partial-thickness burn injury, it
is important to prevent the wound area from bacterial infection with an
antimicrobial dressing. Here, we have tested the antimicrobial properties of
polymeric ultra-thin films composed of poly(lactic acid) (termed "PLA nanosheets"),
which have high flexibility, adhesive strength and transparency, and silver
sulfadiazine (AgSD), which exhibits antimicrobial efficacy. The AgSD-loaded
nanosheet released Ag+ for more than 3 days, and exerted antimicrobial efficacy
against methicillin-resistant Staphylococcus aureus (MRSA) in an in vitro Kirby-
Bauer test. By contrast, a cell viability assay indicated that the dose of AgSD
used in the PLA nanosheets did not show significant cytotoxicity toward
fibroblasts. In vivo evaluation using a mouse model of infection in a partial-
thickness burn wound demonstrated that the nanosheet significantly reduced the
number of MRSA bacteria on the lesion (more than 105-fold) and suppressed the
inflammatory reaction, thereby preventing a protracted wound healing process. ©
2015 Acta Materialia Inc.
AU - Ito, K.
AU - Saito, A.
AU - Fujie, T.
AU - Nishiwaki, K.
AU - Miyazaki, H.
AU - Kinoshita, M.
AU - Saitoh, D.
AU - Ohtsubo, S.
AU - Takeoka, S.
C7 - 3734
DB - Scopus
DO - 10.1016/j.actbio.2015.05.035
KW - Antimicrobial
Poly(lactic acid)
Polymer nanosheet
Silver sulfadiazine
Wound dressing
Animals
Burns
Mice
Nanoparticles
Silver Sulfadiazine
Wound Healing
Bacteria (microorganisms)
Cell culture
Lactic acid
Mammals
Nanosheets
Silver
Ultrathin films
polylactic acid
sulfadiazine silver
antiinfective agent
nanoparticle
Burn injury
Effect of silvers
Methicillin-resistant staphylococcus aureus
Mice models
Poly lactic acid
Polymer nanosheets
Silver sulfadiazines
Wound dressings
animal experiment
animal model
Article
burn infection
cytotoxicity
drug efficacy
drug release
drug screening
fibroblast
film
in vitro study
in vivo study
inflammation
nonhuman
polymerization
priority journal
wound healing
animal
chemistry
drug effects
microbiology
mouse
Bacteria
M3 - Article
PY - 2015
SP - 87-95
ST - Sustainable antimicrobial effect of silver sulfadiazine-loaded nanosheets on
infection in a mouse model of partial-thickness burn injury
TI - Sustainable antimicrobial effect of silver sulfadiazine-loaded nanosheets on
infection in a mouse model of partial-thickness burn injury
84940450427&doi=10.1016%2fj.actbio.2015.05.035&partnerID=40&md5=ffb8d6b489881536d35
3b69a168ef64e
VL - 24
ID - 5574
ER -
TY - JOUR
AB - A progressive way in photodynamic therapy is to design photosensitive agents
that may operate at lower optical power. Hybrid organic-inorganic silver-, iron-,
and silver&iron-contained nanoparticles (NPs) were synthesized using H. perforatum
extract and explored as photosensitizers for anticancer and antimicrobial
photodynamic therapies at ultra-low power light (2.30 mW/cm2). Hybrid silver- and
silver&iron-contained NPs revealed the most promising results for anticancer
photodynamic therapy: at a concentration of 25 μg/mL, the difference between
viability of non-radiated and irradiated HeLa cells was up to 71%. Importantly,
HeLa cancer cells are more assailable for hybrid NP activity than the normal
fibroblast MSU-1.1 cell line, which was observed at both "dark"cytotoxicity and
DCFH-DA assays. Oxidative stress induced by hybrid iron- and silver&iron-contained
NPs in cancer HeLa cells is higher (≥1.7 times) than that in normal fibroblast MSU-
1.1 cells. For antimicrobial photodynamic therapy, hybrid silver&iron-contained NPs
(at a concentration of 25 μg/mL) were found to be promising photosensitizers being
bacteriostatic against E. coli and S. aureus in the "dark"condition and
bactericidal after light irradiation. Antimicrobial properties of hybrid
silver&iron-contained NPs are the result of synergy of antimicrobial activities of
H. perforatum and silver. The highest efficiency against bacteria is achieved at
concentrations and conditions at which H. perforatum and silver NPs apart were less
or not active. Furthermore, hybrid silver&iron-contained NPs combine photodynamic
activities against cancer and bacteria cells with the ability to enhance magnetic
resonance imaging (MRI) contrast. This combination makes these NPs promising
materials for biomedical application at ultra-low power photodynamic therapy of
near-surface cancer or inflammatory disorders, with the possibility of their
visualization using MRI. ©
AU - Ivashchenko, O.
AU - Przysiecka, A.
AU - Peplińska, B.
AU - Flak, D.
AU - Coy, E.
AU - Jarek, M.
AU - Zalewski, T.
AU - Musiał, A.
AU - Jurga, S.
DB - Scopus
DO - 10.1021/acssuschemeng.0c07036
IS - 4
KW - anticancer photodynamic therapy
antimicrobial photodynamic therapy
contrast agent
green synthesis
iron oxide nanoparticles
magnetic resonance imaging
St John's wort
Cell culture
Diseases
Escherichia coli
Fibroblasts
Iron
Lanthanum compounds
Magnetic resonance imaging
Nanoparticles
Photodynamic therapy
Photosensitizers
Hybrid organic-inorganic
Inflammatory disorders
Organic-inorganic hybrid
Photodynamic activities
Photosensitive agents
organic-inorganic materials
M3 - Article
PY - 2021
SP - 1625-1645
ST - Organic-Inorganic Hybrid Nanoparticles Synthesized with Hypericum perforatum
Extract: Potential Agents for Photodynamic Therapy at Ultra-low Power Light
T2 - ACS Sustainable Chemistry and Engineering
TI - Organic-Inorganic Hybrid Nanoparticles Synthesized with Hypericum perforatum
85099941522&doi=10.1021%2facssuschemeng.0c07036&partnerID=40&md5=49ab6989eb3cdd2def
3dde9a3447d5d4
VL - 9
ID - 5217
ER -
TY - JOUR
AB - A progressive way in photodynamic therapy is to design photosensitive agents
that may operate at lower optical power. Hybrid organic-inorganic silver-, iron-,
and silver&iron-contained nanoparticles (NPs) were synthesized using H. perforatum
extract and explored as photosensitizers for anticancer and antimicrobial
photodynamic therapies at ultra-low power light (2.30 mW/cm(2)). Hybrid silver- and
silver&iron-contained NPs revealed the most promising results for anticancer
photodynamic therapy: at a concentration of 25 mu g/mL, the difference between
viability of non-radiated and irradiated HeLa cells was up to 71%. Importantly,
HeLa cancer cells are more assailable for hybrid NP activity than the normal
fibroblast MSU-1.1 cell line, which was observed at both "dark" cytotoxicity and
DCFH-DA assays. Oxidative stress induced by hybrid iron- and silver&iron-contained
NPs in cancer HeLa cells is higher (>= 1.7 times) than that in normal fibroblast
MSU-1.1 cells. For antimicrobial photodynamic therapy, hybrid silver&iron-contained
NPs (at a concentration of 25 mu g/mL) were found to be promising photosensitizers
being bacteriostatic against E. coli and S. aureus in the "dark" condition and
bactericidal after light irradiation. Antimicrobial properties of hybrid
silver&iron-contained NPs are the result of synergy of antimicrobial activities of
H. perforatum and silver. The highest efficiency against bacteria is achieved at
concentrations and conditions at which H. perforatum and silver NPs apart were less
or not active. Furthermore, hybrid silver&iron-contained NPs combine photodynamic
activities against cancer and bacteria cells with the ability to enhance magnetic
resonance imaging (MRI) contrast. This combination makes these NPs promising
materials for biomedical application at ultra-low power photodynamic therapy of
near-surface cancer or inflammatory disorders, with the possibility of their
visualization using MRI.
AN - WOS:000617925200021
AU - Ivashchenko, O.
AU - Przysiecka, L.
AU - Peplinska, B.
AU - Flak, D.
AU - Coy, E.
AU - Jarek, M.
AU - Zalewski, T.
AU - Musial, A.
AU - Jurga, S.
C6 - JAN 2021
DA - FEB 1
DO - 10.1021/acssuschemeng.0c07036
IS - 4
PY - 2021
SN - 2168-0485
SP - 1625-1645
ST - Organic-Inorganic Hybrid Nanoparticles Synthesized with Hypericum perforatum
T2 - ACS SUSTAINABLE CHEMISTRY & ENGINEERING
TI - Organic-Inorganic Hybrid Nanoparticles Synthesized with Hypericum perforatum
VL - 9
ID - 6296
ER -
TY - JOUR
AB - Background Radiotherapy is a common cancer treatment, but often results in
unintended injury to overlying skin and contributes to poor wound healing. The
mechanisms underlying these changes are complex, and existing treatment is limited.
We aimed to systematically review the literature on the pathogenesis, management,
and experimental treatment of delayed wound healing following radiation therapy.
Study Design/Methods A literature search was performed following PRISMA-P
guidelines. PubMed and the Cochrane Library were queried for full-text English
articles published up to 2016 by using key search terms including “radiotherapy”
and “wound healing.” Additional resources were retrieved from reference lists. The
selected articles discussed mechanisms of pathogenesis, current management, and
experimental treatment of radiation-induced skin injury. These are reported as a
qualitative synthesis of the literature from the authors' perspective. Results The
literature search yielded 442 articles, of which 93 were included in this review.
Ionizing radiation causes DNA damage by direct strand breaks and oxygen-derived
free radicals. This insult results in cellular alterations that underlie mechanisms
of delayed wound healing. Prominent theories underlying pathogenesis include
cellular depletion, stromal cell dysfunction, aberrant collagen deposition, and
microvascular damage. Pro-inflammatory cytokines and free radical cascades
contribute to chronic radiation damage, with transforming growth factor beta-1
(TGF-β1) implicated as a key player in the process of fibrosis. At present,
radiation injury is managed medically with conventional wound care with minimal
success and limited evidence-based data. Surgical management with local or free
flap transfer is often compromised by poor surrounding tissue and recipient
vasculature. Experimental treatment models are emerging that target mechanisms of
pathogenesis. These modalities include stem cell repletion, antioxidant therapy,
TGF-β1 modulation, and implantable biomaterials. Conclusion Pathogenesis of delayed
wound healing and fibrosis following radiotherapy is a complex, interdependent
process involving cellular depletion, extracellular matrix changes, microvascular
damage, and altered pro-inflammatory mediators. Current treatment is limited, and
more Level I studies are needed to develop best-practice recommendations.
Investigatory treatment options targeting specific mechanisms of injury may offer
potential solutions to this significant clinical and surgical problem. © 2017 The
Author(s)
AU - Jacobson, L. K.
AU - Johnson, M. B.
AU - Dedhia, R. D.
AU - Niknam-Bienia, S.
AU - Wong, A. K.
DB - Scopus
DO - 10.1016/j.jpra.2017.04.001
KW - Ionizing radiation
Radiation fibrosis
Radiation therapy
Skin
Systematic review
Wound healing
Aloe vera extract
alpha tocopherol
amifostine
ascorbic acid
becaplermin
biomaterial
Calendula extract
corticosteroid derivative
cytokine
DNA
free radical
gamma interferon
halofuginone
histone deacetylase inhibitor
hyaluronic acid
interleukin 1
interleukin 6
nitric oxide
oxygen
pentoxifylline
prostaglandin E1
proteinase
sucralfate
sulfadiazine silver
triethanolamine
vasculotropin
zinc
apoptosis
Article
debridement
DNA damage
DNA strand breakage
experimental therapy
free tissue graft
human
hydrocolloid dressing
hydrogel dressing
hyperbaric oxygen therapy
ionizing radiation
microangiopathy
nonhuman
pathogenesis
practice guideline
qualitative analysis
radiation dermatitis
radiation injury
radiotherapy
skin fibrosis
skin injury
skin protection
stroma cell
systematic review (topic)
tissue flap
treatment response
wound care
M3 - Article
PY - 2017
SP - 92-105
ST - Impaired wound healing after radiation therapy: A systematic review of
pathogenesis and treatment
T2 - JPRAS Open
TI - Impaired wound healing after radiation therapy: A systematic review of
pathogenesis and treatment
85024366523&doi=10.1016%2fj.jpra.2017.04.001&partnerID=40&md5=8d7d8e090ca302513289e
79d39d85ba8
VL - 13
ID - 5468
ER -
TY - JOUR
AB - Tuberculosis (TB) is a highly contagious infection with extensive mortality
and morbidity. The rise of TB-superbugs (drug-resistant strains) with the increase
of their resistance to conventional antibiotics has prompted a further search for
new anti-mycobacterial agents. It is difficult to breach the barriers around TB
bacteria, including mycolic cell wall, granuloma, biofilm and mucus, by
conventional antibiotics in a short span of time. Hence, there is an essential need
for molecules with an uncon-ventional mode of action and structure that can
efficiently break the barriers around mycobacterium. Antimicrobial peptides (AMP)
are essential components of innate immunity having cationic and am-phipathic
characteristics. Lines of evidence show that AMPs have good myco-bactericidal and
anti-biofilm activity against normal as well as antibiotic-resistant TB bacteria.
These peptides have shown direct killing of bacteria by membrane lysis and indirect
killing by activation of innate immune response in host cells by interacting with
the component of the bacterial membrane and intracellular targets through diverse
mechanisms. Despite a good anti-mycobacterial activity, some undesirable
characteristics are also associated with AMP, including hemolysis, cytotoxicity,
susceptibility to proteolysis and poor pharmacokinetic profile, and hence only a
few clinical studies have been conducted with these biomolecules. The design of new
combinatorial therapies, including AMPs and particulate drug delivery systems,
could be new potential alternatives to conventional antibiotics to fight MDR-and
XDR-TB. This review outlined the array of AMP roles in TB therapy, possible
mechanisms of actions, activi-ties, and current advances in pragmatic strategies to
improve challenges accompanying the delivery of AMP for tuberculosis therapeutics.
© 2022 Bentham Science Publishers.
AU - Jadhav, K.
AU - Singh, R.
AU - Ray, E.
AU - Singh, A. K.
AU - Verma, R. K.
DB - Scopus
DO - 10.2174/1389203723666220526161109
IS - 10
KW - Antimicrobial peptides
drug delivery
microparticles
nanoparticles
pro-inflammatory
strategies
tuberculosis
Adenosine Monophosphate
Antimicrobial Cationic Peptides
Antimicrobial Peptides
Bacteria
Humans
Tuberculosis
agents affecting metabolism
antibiotic agent
antimicrobial peptide inducer
aurien1 2 peptide
bacteriocin
beta defensin 1
bictcu5 peptide
bovine neutrophil beta defensin 5 peptide
cationic peptoid
d lak 120
d lak 120 a peptide
d lak 120 hp13 peptide
drug carrier
gold nanoparticle
hh2 peptide
hyaluronic acid nanogel
idr 1018 peptide
indolicidin
inorganic nanoparticle
ip 1 peptide
k4 peptide
lacticin 3147
lassomycin
liposome
llkkk18 peptide
m llkk 2m peptide
magainin 1 analog peptide
mesoporous silica nanoparticle
metal nanoparticle
microsphere
nanogel
nisin
nk 2 peptide
nzx peptide
pep 2 peptide
pin2 peptide
polyglactin microparticle
polyglactin nanoparticle
polymeric microparticle
polypeptide antibiotic agent
porous nano particles aggregates
pr 39
protegrin 1
ropocamptide
silver nanoparticle
trichoderin a
ub2 peptide
unclassified drug
vpamp2 0 peptide
wkwlkkwik peptide
adenosine phosphate
antiinfective agent
antimicrobial cationic peptide
antitubercular activity
drug formulation
dry powder
human
hybrid nano in micro formulation
immunomodulation
Mycobacterium bovis
Mycobacterium marinum
Mycobacterium smegmatis
nonhuman
Short Survey
synergistic effect
bacterium
chemistry
M3 - Short survey
PY - 2022
SP - 643-656
ST - Taming the Devil: Antimicrobial Peptides for Safer TB Therapeutics
T2 - Current Protein and Peptide Science
TI - Taming the Devil: Antimicrobial Peptides for Safer TB Therapeutics
85140481751&doi=10.2174%2f1389203723666220526161109&partnerID=40&md5=72abc235b05735
23d05a09cf20f44788
VL - 23
ID - 5077
ER -
TY - JOUR
AB - According to the World Health Organization report, the increasing antibiotic
resistance of microorganisms is one of the biggest global health problems. The
percentage of bacterial strains showing multidrug resistance (MDR) to commonly used
antibiotics is growing rapidly. Therefore, the search for alternative solutions to
antibiotic therapy has become critical to combat this phenomenon. It is especially
important as frequent and recurring infections can cause cancer. One example of
this phenomenon is urinary tract infections that can contribute to the development
of human urinary bladder carcinoma. This tumor is one of the most common malignant
neoplasms in humans. It occurs almost three times more often in men than in women,
and in terms of the number of cases, it is the fifth malignant neoplasm after
prostate, lung, colon, and stomach cancer. The risk of developing the disease
increases with age. Despite the improvement of its treatment methods, the current
outcome in the advanced stages of this tumor is not satisfactory. Hence, there is
an urgent need to introduce innovative solutions that will prove effective even in
the advanced stage of the disease. In our study, a nanosystem based on ionic silver
(Ag+) bound to a carrier-Titan yellow (TY) was analyzed. The possibility of binding
the thus formed TY-Ag system to Congo red (CR) and albumin (BSA) was determined.
TY-Ag binding to CR provides for better nanosystem solubility and enables its
targeted intracellular transport and binding to immune complexes. The binding of
TY-Ag or CR-TY-Ag to albumin also protects the system against the uncontrolled
release of silver ions. It will also allow the delivery of silver in a targeted
manner directly to the desired site in the case of intravenous administration of
such a system. In this study, the MIC (Minimum Inhibitory Concentration) and MBC
(Minimum Bactericidal Concentration) values of the TY-Ag or BSA-TY-Ag systems were
determined in two reference strains (Escherichia coli and Staphylococcus aureus).
The paper presents nanosystems with a size of about 40-50 nm, with an intense
antibacterial effect obtained at concentrations of 0.019 mM. We have also
discovered that TY-Ag free or complexed with BSA (with a minimal Ag+ dose of 15-20
mu M) inhibited cancer cells proliferation. TY-Ag complex diminished migration and
effectively inhibited the T24 cell viability and induced apoptosis. On the basis of
the obtained results, it has been shown that the presented systems may have anti-
inflammatory and antitumor properties at the same time. TY-Ag or BSA-TY-Ag are new
potential drugs and may become in future important therapeutic compounds in human
urinary bladder carcinoma treatment and/or potent antimicrobial factors as an
alternative to antibiotics.
AN - WOS:000757140300004
AU - Jagusiak, A.
AU - Gosiewski, T.
AU - Romaniszyn, D.
AU - Lasota, M.
AU - Wisniewska, A.
AU - Chlopas, K.
AU - Ostrowska, B.
AU - Koscik, I.
AU - Bulanda, M.
C7 - 26
DA - JAN
DO - 10.3390/ijms23010026
IS - 1
PY - 2022
SN - 1422-0067
ST - Antibacterial Therapy by Ag+ Ions Complexed with Titan Yellow/Congo Red and
Albumin during Anticancer Therapy of Urinary Bladder Cancer
TI - Antibacterial Therapy by Ag+ Ions Complexed with Titan Yellow/Congo Red and
Albumin during Anticancer Therapy of Urinary Bladder Cancer
VL - 23
ID - 6429
ER -
TY - JOUR
AB - According to the World Health Organization report, the increasing antibiotic
resistance of microorganisms is one of the biggest global health problems. The
percentage of bacterial strains showing multidrug resistance (MDR) to commonly used
antibiotics is growing rapidly. Therefore, the search for alternative solutions to
antibiotic therapy has become critical to combat this phenom-enon. It is especially
important as frequent and recurring infections can cause cancer. One example of
this phenomenon is urinary tract infections that can contribute to the development
of human urinary bladder carcinoma. This tumor is one of the most common malignant
neoplasms in humans. It occurs almost three times more often in men than in women,
and in terms of the number of cases, it is the fifth malignant neoplasm after
prostate, lung, colon, and stomach cancer. The risk of devel-oping the disease
increases with age. Despite the improvement of its treatment methods, the current
outcome in the advanced stages of this tumor is not satisfactory. Hence, there is
an urgent need to introduce innovative solutions that will prove effective even in
the advanced stage of the disease. In our study, a nanosystem based on ionic silver
(Ag+) bound to a carrier—Titan yellow (TY) was analyzed. The possibility of binding
the thus formed TY‐Ag system to Congo red (CR) and albumin (BSA) was determined.
TY‐Ag binding to CR provides for better nanosystem solubility and enables its
targeted intracellular transport and binding to immune complexes. The binding of
TY‐Ag or CR‐ TY‐Ag to albumin also protects the system against the uncontrolled
release of silver ions. It will also allow the delivery of silver in a targeted
manner directly to the desired site in the case of intravenous administration of
such a system. In this study, the MIC (Minimum Inhibitory Concentration) and MBC
(Minimum Bactericidal Concentration) values of the TY‐Ag or BSA‐TY‐Ag systems were
determined in two reference strains (Escherichia coli and Staphylococcus aureus).
The paper presents nanosystems with a size of about 40–50 nm, with an intense
antibacterial effect obtained at concentrations of 0.019 mM. We have also
discovered that TY‐Ag free or complexed with BSA (with a minimal Ag+ dose of 15–20
μM) inhibited cancer cells proliferation. TY‐Ag complex diminished mi-gration and
effectively inhibited the T24 cell viability and induced apoptosis. On the basis of
the obtained results, it has been shown that the presented systems may have anti‐
inflammatory and antitumor properties at the same time. TY‐Ag or BSA‐TY‐Ag are new
potential drugs and may become in future important therapeutic compounds in human
urinary bladder carcinoma treatment and/or potent antimicrobial factors as an
alternative to antibiotics. © 2021 by the authors. Licensee MDPI, Basel,
Switzerland.
AU - Jagusiak, A.
AU - Gosiewski, T.
AU - Romaniszyn, D.
AU - Lasota, M.
AU - Wiśniewska, A.
AU - Chłopaś, K.
AU - Ostrowska, B.
AU - Kościk, I.
AU - Bulanda, M.
C7 - 26
DB - Scopus
DO - 10.3390/ijms23010026
IS - 1
KW - Apoptosis
Bovine serum albumin
Congo red
Escherichia coli
Migration
Minimum bactericidal concentration
Minimum inhibitory concentration
Necrosis
Silver ions
T24 cell line
Titan yellow
Tumor growth
antibiotic agent
antiinfective agent
caspase 3
congo red
drug carrier
dye
paclitaxel
silver
titan yellow
unclassified drug
agar gel electrophoresis
antibiotic therapy
antigen antibody complex
apoptosis
Article
bacterial growth
bacterial strain
bladder cancer
cancer cell
cancer therapy
cell cycle G1 phase
cell cycle G2 phase
cell cycle S phase
cell migration
cell proliferation
cell survival
cell viability
comparative study
complex formation
controlled study
drug cytotoxicity
drug efficacy
drug formulation
drug solubility
drug synthesis
enzyme activation
gel filtration chromatography
growth inhibition
human
human cell
necrosis
nonhuman
M3 - Article
PY - 2022
ST - Antibacterial therapy by Ag+ ions complexed with titan yellow/congo red and
albumin during anticancer therapy of urinary bladder cancer
TI - Antibacterial therapy by Ag+ ions complexed with titan yellow/congo red and
albumin during anticancer therapy of urinary bladder cancer
85121378702&doi=10.3390%2fijms23010026&partnerID=40&md5=fea5438cf86e912670fe7d3d84c
990b3
VL - 23
ID - 5148
ER -
TY - JOUR
AB - Aim: To evaluate the anti inflammatory activity of silver nanoparticles
synthesised using Cumin oil. Introduction: Silver nanoparticles are the most
generally utilised nanoparticles both in key therapeutic sciences and clinical
practice. Silver particles are connected to modified cell demise, and expanded
cytotoxicity in specific conditions. Materials and method: Preparation of
denaturation particles using Cumin oil and evaluating the anti inflammatory
activity by using albumin denaturation assay technique. Results: The characteristic
surface plasmon absorption band were observed at 440nm. It is observed that with
the increase in concentration, the anti-inflammatory activity of silver
nanoparticles increases. Conclusion: Silver nanoparticles synthesized using cumin
oil exhibited potent anti inflammatory activity and hence can be used along with
anti inflammatory drugs, which would potentiate the effect of the drugs. © RJPT All
right reserved.
AU - Jain, A.
AU - Anitha, R.
DB - Scopus
DO - 10.5958/0974-360X.2019.00469.4
IS - 6
KW - Anti inflammatory
Biosynthesis
Cumin oil
UV-vis spectroscopy
cumin oil
silver nanoparticle
unclassified drug
vegetable oil
Article
cumin
drug analysis
drug isolation
drug synthesis
nanomedicine
plant leaf
protein denaturation
M3 - Article
PY - 2019
SP - 2790-2793
ST - Anti inflammatory activity of silver nanoparticles synthesised using cumin
oil
T2 - Research Journal of Pharmacy and Technology
TI - Anti inflammatory activity of silver nanoparticles synthesised using cumin
oil
85074811851&doi=10.5958%2f0974-
360X.2019.00469.4&partnerID=40&md5=6301200f8d69ad313a0812203507cc59
VL - 12
ID - 5366
ER -
TY - CONF
AB - Silver is an effective antimicrobial agent with low toxicity, which is
important especially in the treatment of burn wounds where transient bacteremia is
prevalent and its fast control is essential. Drugs releasing silver in ionic forms
are known to get neutralized in biological fluids and upon long-term use may cause
cosmetic abnormality, e.g., argyria and delayed wound healing. Given its broad
spectrum activity, efficacy and lower costs, the search for newer and superior
silver based antimicrobial agents is necessary. Among the various options
available, silver nanoparticles have been the focus of increasing interest and are
being heralded as an excellent candidate for therapeutic purposes. This report
gives an account of our work on development of an antimicrobial gel formulation
containing silver nanoparticles (SNP) in the size range of 7-20 nm synthesized by a
proprietary biostabilization process. The typical minimum inhibitory concentration
(MIC) and minimum bactericidal concentration (MBC) against standard reference
cultures as well as multidrug-resistant organisms were 0.78-6.25 μg/mL and 12.5
μg/mL, respectively. Gram-negative bacteria were killed more effectively (3 log10
decrease in 5-9 h) than Grampositive bacteria (3 log10 decrease in 12 h). SNP also
exhibited good antifungal activity (50% inhibition at 75 μg/mL with antifungal
index 55.5% against Aspergillus niger and MIC of 25 μg/mL against Candida
albicans). When the interaction of SNP with commonly used antibiotics was
investigated, the observed effects were synergistic (ceftazidime), additive
(streptomycin, kanamycin, ampiclox, polymyxin B) and antagonistic
(chloramphenicol). Interestingly,SNPexhibited good anti-inflammatory properties as
indicated by concentration-dependent inhibition of marker enzymes (matrix
metalloproteinase 2 and 9). The post agent effect (a parameter measuring the length
of time for which bacterial growth remains suppressed following brief exposure to
the antimicrobial agent) varied with the type of organism (e.g., 10.5 h for P.
aeruginosa, 1.3 h for Staphylococcus sp. and 1.6 h for Candida albicans) indicating
that dose regimen of the SNP formulation should ensure sustained release of the
drug. To meet this requirement, a gel formulation containing SNP (S-gel) was
prepared. The antibacterial spectrum of S-gel was found to be comparable to that of
a commercial formulation of silver sulfadiazine, albeit at a 30-fold less silver
concentration. As part of toxicity studies, localization of SNP in Hep G2 cell
line, cell viability, biochemical effects and apoptotic/necrotic potential were
assessed. It was found that SNP get localized in the mitochondria and have an IC50
value of 251 μg/mL. Even though they elicit an oxidative stress, cellular
antioxidant systems (reduced glutathione content, superoxide dismutase, catalase)
get triggered and prevent oxidative damage. Further, SNP induce apoptosis at
concentrations up to 250 μg/mL, which could favor scarless wound healing. Acute
dermal toxicity studies on SNP gel formulation (S-gel) in Sprague-Dawley rats
showed complete safety for topical application. These results clearly indicate that
silver nanoparticles could provide a safer alternative to conventional
antimicrobial agents in the form of a topical antimicrobial formulation. © 2009
AU - Jain, J.
AU - Arora, S.
AU - Rajwade, J. M.
AU - Omray, P.
AU - Khandelwal, S.
AU - Paknikar, K. M.
DB - Scopus
DO - 10.1021/mp900056g
ET - 5
KW - Antimicrobial
Dermal toxicity
Formulation
Animals
Antioxidants
Cell Survival
Gels
Hep G2 Cells
Humans
Metal Nanoparticles
Nanotechnology
Rats
Silver
Skin
ampicillin plus cloxacillin
catalase
ceftazidime
chloramphenicol
gelatinase A
gelatinase B
glutathione
kanamycin
nanoparticle
polymyxin B
silver
streptomycin
sulfadiazine silver
animal experiment
antifungal activity
Aspergillus nidulans
bacterial growth
Candida albicans
conference paper
controlled study
drug antagonism
drug formulation
drug potentiation
enzyme inhibition
female
gel
human
human cell
male
nonhuman
oxidative stress
particle size
priority journal
rat
Staphylococcus
toxicity testing
PY - 2009
SP - 1388-1401
ST - Silver nanoparticles in therapeutics: Development of an antimicrobial gel
formulation for topical use
T2 - Molecular Pharmaceutics
TI - Silver nanoparticles in therapeutics: Development of an antimicrobial gel
formulation for topical use
70350051319&doi=10.1021%2fmp900056g&partnerID=40&md5=b8f484465009a59a36a35322b82896
e5
VL - 6
ID - 5785
ER -
TY - JOUR
AB - Silver is an effective antimicrobial agent with low toxicity, which is
important especially in the treatment of burn wounds where transient bacteremia is
prevalent and its fast control is essential. Drugs releasing silver in ionic forms
are known to get neutralized in biological fluids and upon long-term use may cause
cosmetic abnormality, e.g., argyria and delayed wound healing. Given its broad
spectrum activity, efficacy and lower costs, the search for newer and superior
silver based antimicrobial agents is necessary. Among the various options
available, silver nanoparticles have been the focus of increasing interest and are
being heralded as an excellent candidate for therapeutic purposes. This report
gives an account of our work on development of an antimicrobial gel formulation
containing silver nanoparticles (SNP) in the size range of 7-20 nm synthesized by a
proprietary biostabilization process. The typical minimum inhibitory concentration
(MIC) and minimum bactericidal concentration (MBC) against standard reference
cultures as well as multidrug-resistant organisms were 0.78-6.25 mu g/mL and 12.5
mu g/mL, respectively. Gram-negative bacteria were killed more effectively (3
log(10) decrease in 5-9 h) than Gram-positive bacteria (3 log(10) decrease in 12
h). SNP also exhibited good antifungal activity (50% inhibition at 75 mu g/mL with
antifungal index 55.5% against Aspergillus niger and MIC of 25 mu g/mL against
Candida albicans), When the interaction of SNP with commonly used antibiotics was
investigated, the observed effects were synergistic (ceftazidime), additive
(streptomycin, kanamycin, ampiclox, polymyxin B) and antagonistic
(chloramphenicol), Interestingly, SNIP exhibited good anti-inflammatory properties
as indicated by concentration-dependent inhibition of marker enzymes (matrix
metalloproteinase 2 and 9). The post agent effect (a parameter measuring the length
of time for which bacterial growth remains suppressed following brief exposure to
the antimicrobial agent) varied with the type of organism (e.g., 10.5 h for P.
aeruginosa, 1.3 h for Staphylococcus sp. and 1.6 h for Candida albicans) indicating
that dose regimen of the SNIP formulation should ensure sustained release of the
drug. To meet this requirement, a gel formulation containing SNP (S-gel) was
prepared. The antibacterial spectrum of S-gel was found to be comparable to that of
a commercial formulation of silver sulfadiazine, albeit at a 30-fold less silver
concentration. As part of toxicity studies, localization of SNIP in Hep G2 cell
line, cell viability, biochemical effects and apoptotic/necrotic potential were
assessed. It was found that SNP get localized in the mitochondria and have an IC50
value of 251 mu g/mL. Even though they elicit an oxidative stress, cellular
antioxidant systems (reduced glutathione content, superoxide dismutase, catalase)
get triggered and prevent oxidative damage. Further, SNIP induce apoptosis at
concentrations up to 250 mu g/mL, which could favor scarless wound healing. Acute
dermal toxicity studies on SNIP gel formulation (S-gel) in Sprague-Dawley rats
showed complete safety for topical application. These results clearly indicate that
silver nanoparticles could provide a safer alternative to conventional
antimicrobial agents in the form of a topical antimicrobial formulation.
AN - WOS:000270354400015
AU - Jain, J.
AU - Arora, S.
AU - Rajwade, J. M.
AU - Omray, P.
AU - Khandelwal, S.
AU - Paknikar, K. M.
DA - SEP-OCT
DO - 10.1021/mp900056g
IS - 5
PY - 2009
SN - 1543-8384
1543-8392
SP - 1388-1401
ST - Silver Nanoparticles in Therapeutics: Development of an Antimicrobial Gel
Formulation for Topical Use
T2 - MOLECULAR PHARMACEUTICS
TI - Silver Nanoparticles in Therapeutics: Development of an Antimicrobial Gel
Formulation for Topical Use
VL - 6
ID - 5884
ER -
TY - JOUR
AB - Antibiotic resistance has necessitated search for new antibacterials for
combating threat of pathogenic bacteria. Though chemically synthesized silver
nanoparticles are a well-known antimicrobial agent, they are toxic to human cells
at higher concentrations. Hence in the present study, curcumin-silver nanoparticles
(Cur–AgNPs) of size 25–35 nm, were synthesized using curcumin, a phytochemical.
These nanoparticles were effective against both Gram positive and Gram-negative
bacteria and were less toxic to human keratinocytes. They had very low total silver
content and high stability. The antibacterial activity of Cur–AgNPs, as studied by
minimum inhibitory concentration (MIC = 5 mg/L), time kill kinetics and post agent
effect, was better than silver nanoparticles (AgNPs, size ≈ 35 nm, MIC = 20 mg/L).
The inhibitory effect of Cur–AgNPs on biofilm formation was also ≈ 20% more than
AgNPs as demonstrated by live–dead imaging and scanning electron microscopy. The
cytotoxic test to skin keratinocytes (HaCaT) showed that Cur–AgNPs were toxic at a
concentration of 156 mg/L which is much higher than the bacterial MIC (selective
toxicity). They also showed anti-inflammatory effect on human macrophages (THP1) by
reducing secretion of pro-inflammatory cytokines IL-6 and TNF-α as compared to
chemically synthesized AgNPs. © 2017, Springer-Verlag GmbH Germany.
AU - Jaiswal, S.
AU - Mishra, P.
DB - Scopus
DO - 10.1007/s00430-017-0525-y
IS - 1
KW - Antibacterial
Antibiofilm
Curcumin
Macrophage
Selective toxicity
Silver nanoparticle
Biofilms
Cell Line
Cell Survival
Cytokines
Humans
Keratinocytes
Macrophages
Microbial Viability
Nanoparticles
Silver
curcumin
cytokine
interleukin 6
silver nanoparticle
antiinfective agent
nanoparticle
silver
Article
bacterial cell
biofilm
cytokine release
drug cytotoxicity
drug stability
drug synthesis
HaCat cell line
human
human cell
keratinocyte
mammal cell
nonhuman
particle size
priority journal
THP-1 cell line
cell line
cell survival
drug effect
macrophage
microbial viability
physiology
secretion (process)
M3 - Article
PY - 2018
SP - 39-53
ST - Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with
improved stability and selective toxicity to bacteria over mammalian cells
T2 - Medical Microbiology and Immunology
TI - Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with
improved stability and selective toxicity to bacteria over mammalian cells
85032390225&doi=10.1007%2fs00430-017-0525-
y&partnerID=40&md5=0753d6b5cf86a30c97f71dfc45318ee9
VL - 207
ID - 5480
ER -
TY - JOUR
AB - Urban air particulate pollution is a known cause for adverse human health
effects worldwide. China has encountered air quality problems in recent years due
to rapid industrialization. Toxicological effects induced by particulate air
pollution vary with particle sizes and season. However, it is not known how
distinctively different photochemical activity and different emission sources
during the day and the night affect the chemical composition of the PM size ranges
and subsequently how it is reflected to the toxicological properties of the PM
exposures. The particulate matter (PM) samples were collected in four different
size ranges (PM10-2.5; PM2.5-1; PM1-0.2 and PM0.2) with a high volume cascade
impactor. The PM samples were extracted with methanol, dried and thereafter used in
the chemical and toxicological analyses. RAW264.7 macrophages were exposed to the
particulate samples in four different doses for 24 h. Cytotoxicity, inflammatory
parameters, cell cycle and genotoxicity were measured after exposure of the cells
to particulate samples. Particles were characterized for their chemical
composition, including ions, element and PAH compounds, and transmission electron
microscopy (TEM) was used to take images of the PM samples. Chemical composition
and the induced toxicological responses of the size segregated PM samples showed
considerable size dependent differences as well as day to night variation. The
PM10-2.5 and the PM0.2 samples had the highest inflammatory potency among the size
ranges. Instead, almost all the PM samples were equally cytotoxic and only minor
differences were seen in genotoxicity and cell cycle effects. Overall, the PM0.2
samples had the highest toxic potential among the different size ranges in many
parameters. PAH compounds in the samples and were generally more abundant during
the night than the day, indicating possible photo-oxidation of the PAH compounds
due to solar radiation. This was reflected to different toxicity in the PM samples.
Some of the day to night difference may have been caused also by differing wind
directions transporting air masses from different emission sources during the day
and the night. The present findings indicate the important role of the local
particle sources and atmospheric processes on the health related toxicological
properties of the PM. The varying toxicological responses evoked by the PM samples
showed the importance of examining various particle sizes. Especially the detected
considerable toxicological activity by PM0.2 size range suggests they're
attributable to combustion sources, new particle formation and atmospheric
processes. © 2015 Elsevier Ltd.
AU - Jalava, P. I.
AU - Wang, Q.
AU - Kuuspalo, K.
AU - Ruusunen, J.
AU - Hao, L.
AU - Fang, D.
AU - Väisänen, O.
AU - Ruuskanen, A.
AU - Sippula, O.
AU - Happo, M. S.
AU - Uski, O.
AU - Kasurinen, S.
AU - Torvela, T.
AU - Koponen, H.
AU - Lehtinen, K. E. J.
AU - Komppula, M.
AU - Gu, C.
AU - Jokiniemi, J.
AU - Hirvonen, M. R.
DB - Scopus
DO - 10.1016/j.atmosenv.2015.08.089
KW - Air quality
Cytotoxicity
In vitro
Inflammation
Particulate matter
Toxicology
China
Air pollution
Atmospheric chemistry
Chemical analysis
Chemical compounds
Meteorological instruments
Particle size
Pollution
Toxicity
aluminum
ammonium nitrate
ammonium sulfate
arsenic
barium ion
cadmium
calcium ion
chromium
copper
ferric ion
iron
lead
magnesium
manganese
metal
molybdenum
nickel
nitrate
potassium
rubidium ion
selenium
silver
sodium
strontium
sulfate
titanium
vanadium
zinc
zinc ion
In-vitro
Particulate air pollution
Particulate Matter
Photochemical activity
Toxicological analysis
Toxicological properties
air mass
air quality
air sampling
atmospheric pollution
genotoxicity
health risk
industrialization
PAH
particle size
particulate matter
phytochemistry
public health
sampler
solar radiation
toxicology
urban atmosphere
wind direction
air pollution
animal cell
Article
cell cycle
controlled study
cytotoxicity
inflammation
macrophage
mouse
nonhuman
photooxidation
priority journal
Polycyclic aromatic hydrocarbons
M3 - Article
PY - 2015
SP - 427-437
ST - Day and night variation in chemical composition and toxicological responses
of size segregated urban air PM samples in a high air pollution situation
T2 - Atmospheric Environment
TI - Day and night variation in chemical composition and toxicological responses
of size segregated urban air PM samples in a high air pollution situation
84942013194&doi=10.1016%2fj.atmosenv.2015.08.089&partnerID=40&md5=45397688f45fe0da8
01197076f2ddcb5
VL - 120
ID - 5581
ER -
TY - JOUR
AB - In the current study for the first time, silver nanoparticles (AgNPs) were
biosynthesized by reducing agents from hot water extract of Allium ampeloprasum, an
antibacterial and anti-inflammatory edible plant. UV–vis. spectroscopy, Fourier
transform infrared spectroscopy, dynamic light scattering, X-ray diffractometric,
and transmission electron microscopy (TEM) analyses have been applied to confirm
the formation of biosynthesized AgNPs. Total phenol content and antioxidant
activities of AgNPs and extract together with their antibacterial and cytotoxic
properties, were evaluated. According to TEM, AgNPs were spherical with a diameter
of 8–50 nm. Total phenolic compounds were 15.58 μg/mL, and 10.94 μg/mL at a
concentration of 150 μg/mL for the A. ampeloprasum extract and the biosynthesized
AgNPs, respectively. Biosynthesized AgNPs showed significant antioxidant activity
(81%) as compared to A. ampeloprasum extract (32%) and were active on multi-drug
resistant P. aeruginosa. Besides, the cytotoxic activity response was also
demonstrated that AgNPs were more potent than the A. ampeloprasum extract and
showed high activity against Hela cell line with an IC50 value of less than 25
µg/mL. In conclusion, AgNPs synthesized by A. ampeloprasum extract with excellent
antioxidant and antibacterial effects and acceptable cytotoxicity on cervical
cancer cells have the potential to be used in biological applications. © 2020
Society of Powder Technology Japan
AU - Jalilian, F.
AU - Chahardoli, A.
AU - Sadrjavadi, K.
AU - Fattahi, A.
AU - Shokoohinia, Y.
DB - Scopus
DO - 10.1016/j.apt.2020.01.011
IS - 3
KW - Allium ampeloprasum
Antibacterial
Antioxidant
Cytotoxicity
Antioxidants
Cell culture
Drug delivery
Light scattering
Metal nanoparticles
Phenols
Reducing agents
Biological applications
Cervical cancer cells
Total phenol contents
Total phenolic compounds
Plants (botany)
M3 - Article
PY - 2020
SP - 1323-1332
ST - Green synthesized silver nanoparticle from Allium ampeloprasum aqueous
extract: Characterization, antioxidant activities, antibacterial and cytotoxicity
effects
T2 - Advanced Powder Technology
TI - Green synthesized silver nanoparticle from Allium ampeloprasum aqueous
extract: Characterization, antioxidant activities, antibacterial and cytotoxicity
effects
85078306969&doi=10.1016%2fj.apt.2020.01.011&partnerID=40&md5=f4318fa3775ff98709bdde
dd40c4eddb
VL - 31
ID - 5349
ER -
TY - JOUR
AB - Background There is growing interest in the role of microbes in the
pathogenesis of coronary atherosclerosis but most of the evidence has been
seroepidemiologic. It would be useful to know more about the cytology and histology
of coronary lesions containing clearly depicted microbes. Objective To define
carefully the assorted abnormalities apparent in the coronary arteries of
individuals dying with Whipple's disease. Methods Myocardial tissue from 12 cases
of Whipple's disease was studied by light microscopy. Slides were stained routinely
(in sequence) with either the periodic-acid-Schiff (PAS) or Goldner-trichrome
method and some with Gomori methenimine silver. Cardiac slides with PAS-positive
bacilli were compared to lesions in jejunal lamina propria. Results There were
abundant sites of coronary arterial damage associated with presence of Whipple
bacilli, more in the tunica media than in intima and adventitia. Bacilli in the
arterial lesions were identical to those in lamina propria. Medial lesions were
often associated with a fibroproliferative 'atheroma'. Both intracellular and
extracellular bacilli were found. Most lesions were devoid of inflammation, but
some sites exhibited either florid arteritis or dense scarring. Arteries that were
scarred or inflamed exhibited only a few bacilli. There was an apparent affinity of
bacilli for the nuclei in medial smooth muscle cells and in nearby ventricular
myocytes. Apoptosis (TUNEL-positive) was present in medial smooth muscle cells,
endothelial cells, and ventricular myocytes. Conclusions There is a wide spectrum
of coronary abnormalities in Whipple's disease. It would be useful to know how
often the Whipple bacillus is a part of the total pathogen burden in coronary
disease. Coron Artery Dis 12:115-125 (C) 2001 Lippincott Williams & Wilkins.
AN - WOS:000167693200005
AU - James, T. N.
DA - MAR
DO - 10.1097/00019501-200103000-00005
IS - 2
PY - 2001
SN - 0954-6928
1473-5830
SP - 115-125
ST - On the wide spectrum of abnormalities in the coronary arteries of Whipple's
disease
T2 - CORONARY ARTERY DISEASE
TI - On the wide spectrum of abnormalities in the coronary arteries of Whipple's
disease
VL - 12
ID - 6684
ER -
TY - JOUR
AB - This work describes the traditional wet and green synthetic approaches,
structural features, and extensive bioactivity study for a new coordination polymer
[Ag(mu-PTA)(Df)(H2O)] n center dot 3nH(2)O (1) that bears a silver(I) center, a
1,3,5-triaza-phosphaadamantane (PTA) linker, and a nonsteroidal anti-inflammatory
drug, diclofenac (Df-). Compared to cisplatin, compound 1 exhibits both anti-
inflammatory properties and very remarkable cytotoxicity toward various cancer cell
lines with a high value of selectivity index. Additionally, the 3D model
representing human pancreas/duct carcinoma (PANC-1) and human lung adenocarcinoma
(A549) was designed and applied as a clear proof of the remarkable therapeutic
potential of 1. The obtained experimental data indicate that 1 induces an apoptotic
pathway via reactive oxygen species generation, targeting mitochondria due to their
membrane depolarization. This study broadens a group of bioactive metal-organic
networks and highlights the significant potential of such compounds in developing
advanced therapeutic solutions.
AN - WOS:000896299300001
AU - Jaros, S. W.
AU - Komarnicka, U. K.
AU - Kyziol, A.
AU - Pucelik, B.
AU - Nesterov, D. S.
AU - Kirillov, A. M.
AU - Smolenski, P.
DA - AUG 25
IS - 16
PY - 2022
SN - 0022-2623
1520-4804
SP - 11100-11110
ST - Therapeutic Potential of a Water-Soluble Silver-Diclofenac Coordination
Polymer on 3D Pancreatic Cancer Spheroids
T2 - JOURNAL OF MEDICINAL CHEMISTRY
TI - Therapeutic Potential of a Water-Soluble Silver-Diclofenac Coordination
VL - 65
ID - 6351
ER -
TY - JOUR
AB - This work describes the traditional wet and green synthetic approaches,
structural features, and extensive bioactivity study for a new coordination polymer
[Ag(μ-PTA)(Df)(H2O)]n·3nH2O (1) that bears a silver(I) center, a 1,3,5-triaza-
phosphaadamantane (PTA) linker, and a nonsteroidal anti-inflammatory drug,
diclofenac (Df-). Compared to cisplatin, compound 1 exhibits both anti-inflammatory
properties and very remarkable cytotoxicity toward various cancer cell lines with a
high value of selectivity index. Additionally, the 3D model representing human
pancreas/duct carcinoma (PANC-1) and human lung adenocarcinoma (A549) was designed
and applied as a clear proof of the remarkable therapeutic potential of 1. The
obtained experimental data indicate that 1 induces an apoptotic pathway via
reactive oxygen species generation, targeting mitochondria due to their membrane
depolarization. This study broadens a group of bioactive metal-organic networks and
highlights the significant potential of such compounds in developing advanced
therapeutic solutions. © 2022 American Chemical Society. All rights reserved.
AU - Jaros, S. W.
AU - Komarnicka, U. K.
AU - Kyzioł, A.
AU - Pucelik, B.
AU - Nesterov, D. S.
AU - Kirillov, A. M.
AU - Smoleński, P.
DB - Scopus
IS - 16
KW - Antineoplastic Agents
Cell Line, Tumor
Diclofenac
Humans
Pancreatic Neoplasms
Polymers
Silver
Water
1,3,5 triazaphosphaadamantane
cisplatin
diclofenac
polymer
silver
silver diclofenac coordination polymer
unclassified drug
water
A-549 cell line
apoptosis
Article
biological activity
cancer cell line
controlled study
crystal structure
drug cytotoxicity
drug design
drug formulation
drug structure
drug synthesis
elemental analysis
green chemistry
human
human cell
IC50
lung adenocarcinoma
membrane depolarization
mitochondrion
PANC-1 cell line
pancreas cancer
pancreatic ductal carcinoma
selectivity index
tumor spheroid
water solubility
chemistry
pancreas tumor
tumor cell line
M3 - Article
PY - 2022
SP - 11100-11110
ST - Therapeutic Potential of a Water-Soluble Silver-Diclofenac Coordination
T2 - Journal of Medicinal Chemistry
TI - Therapeutic Potential of a Water-Soluble Silver-Diclofenac Coordination
85136657954&doi=10.1021%2facs.jmedchem.2c00535&partnerID=40&md5=e2901a61e3fb0b08889
0abe5f0bc986a
VL - 65
ID - 5122
ER -
TY - JOUR
AB - Gold nanoparticles (Au NPs) are used in diagnostic and therapeutic
applications together with a variety of industrial purposes and in many biomedical
sectors with potential risks to human health. The present study aimed to the
histological, histochemical, and ultrastructural alterations induced by Au NPS in
vital organs. Healthy male Wistar Albino rats (Rattus norvegicus) were subjected to
20 injections of 10-nm Au NPs at a daily dose of 2 mg/kg. Liver, kidney, heart, and
lung biopsies from control and Au NPs-treated rats under study were subjected to
histological and histochemical examinations. In comparison with the control rats,
the renal tissue of Au NPs-treated rats demonstrated glomerular congestion,
interstitial inflammatory cell infiltration, renal tubular hydropic degeneration,
cloudy swelling, necrosis, and hyaline cast precipitation. In addition, Au NPs
induced the following hepatic alterations: hepatocyte cytolysis, cytoplasmic
vacuolation, hydropic degeneration, and nuclear alterations together with
sinusoidal dilatation. Moreover, the hearts of the treated rats demonstrated
myocarditis, cardiac congestion, hyalinosis, cardiomyocyte hydropic degeneration,
myofiber disarray and cardiac congestion. The lungs of Au NPs-treated rats also
exhibited the following pulmonary alterations: alectasis, emphysema, inflammatory
cell inflammation, thickened alveolar walls, pulmonary interstitial edema,
congestion, hypersensitivity, fibrocyte proliferation, and honeycombing. In
conclusion, exposure to Au NPs induced histological, histochemical and
ultrastructural alterations in the vital organs that may alter the function of
these organs. Additional efforts are needed for better understanding the potential
risks of Au NPs to human health.
AN - WOS:000869422000001
AU - Jarrar, Q.
AU - Al-Doaiss, A.
AU - Jarrar, B. M.
AU - Alshehri, M.
C6 - OCT 2022
DA - DEC
DO - 10.1177/07482337221133881
IS - 12
PY - 2022
SN - 0748-2337
1477-0393
SP - 789-800
ST - On the toxicity of gold nanoparticles: Histological, histochemical and
ultrastructural alterations
TI - On the toxicity of gold nanoparticles: Histological, histochemical and
ultrastructural alterations
VL - 38
ID - 6474
ER -
TY - JOUR
AB - Metallic nanoparticles (NPs) are widely used in medical preparations. The
present study aims to find out the influence of widely used five metallic NPs on
the expression of major hepatic drug-metabolizing enzyme (DME) genes. Six groups of
BALB/C mice, 7 mice each, were exposed to: Gold NPs, silver NPs, copper oxide NPs,
silicon dioxide NPs and zinc oxide NPs, for 21 days. Liver biopsies from all mice
were subjected to mouse cyp3a11, cyp2c29, ugt2b1 and interleukin-6 (il6) gene
expression quantification using real-time polymerase chain reaction, in addition to
inflammatory cell infiltration examination. All tested NPs caused a sharp and
significant (ANOVA, p value <0.05) downregulation in the expression of DME genes,
with the highest influence was observed in mice exposed to copper oxide NPs.
Additionally, all NPs induced hepatic inflammation and upregulated the expression
of il6 gene, which were inversely correlated with the expression of DMEs. It is
concluded that all tested NPs downregulated the expression of DME genes, with the
highest influence exhibited by copper oxide NPs, in correlation with inflammation
and il6 gene induction in the liver. Further studies are needed to find out the
effect of anti-inflammatory compounds against the alterations induced by metallic
NPs exposure on hepatic DMEs. © 2020 Elsevier B.V.
AU - Jarrar, Y.
AU - Al-Doaiss, A.
AU - Alfaifi, M.
AU - Shati, A.
AU - Al-Kahtani, M.
AU - Jarrar, B.
C7 - 103449
DB - Scopus
DO - 10.1016/j.etap.2020.103449
KW - Drug metabolizing enzymes
Gene expression
Interleukin 6
Liver
Nanobiology
Nanoparticles
Animals
Cytochrome P-450 CYP2C9
Cytochrome P-450 CYP3A
Down-Regulation
Gene Expression
Inflammation
Interleukin-6
Male
Metabolic Clearance Rate
Metal Nanoparticles
Mice, Inbred BALB C
cytochrome P450 3A11
drug metabolizing enzyme
gold nanoparticle
interleukin 6
metal nanoparticle
silica nanoparticle
silver nanoparticle
cytochrome P450 2C9
cytochrome P450 3A
cytochrome P450 3A4, mouse
adult
animal cell
animal experiment
animal tissue
Article
Bagg albino mouse
cell infiltration
controlled study
cyp2c29 gene
cyp3a11 gene
down regulation
exposure
gene expression
hepatitis
il6 gene
inflammatory cell
liver biopsy
male
mouse
nonhuman
priority journal
toxicity
ugt2b1 gene
upregulation
animal
drug effect
enzymology
genetics
immunology
inflammation
liver
metabolic clearance rate
pathology
pharmacokinetics
pollutant
M3 - Article
PY - 2020
ST - The influence of five metallic nanoparticles on the expression of major drug-
metabolizing enzyme genes with correlation of inflammation in mouse livers
TI - The influence of five metallic nanoparticles on the expression of major drug-
metabolizing enzyme genes with correlation of inflammation in mouse livers
85086942302&doi=10.1016%2fj.etap.2020.103449&partnerID=40&md5=954d47809e76f5690cdaa
88486bf8fa5
VL - 80
ID - 5258
ER -
TY - JOUR
AB - Biosynthesis of zinc oxide nanoparticles (ZnO-NPs) was achieved by utilizing
the reducing and capping potential of leaf, stem and callus aqueous extracts of
Mussaenda frondosa.The bioreduced ZnO-NPs were characterized using powder X-ray
diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis spectroscopy), scanning
electron microscopy (SEM), energy dispersive spectroscopy (EDS), fourier transform
infrared spectroscopy (FTIR) and dynamic light scattering (DLS) techniques. UV-
visible spectra of ZnO-NPs showed a strong absorption peak at 370, 376 and 373 nm
corresponding to the band gap energy of 3.33, 3.27 and 3.30 eV for ZnO-NPs obtained
from leaf (L-ZnO-NP), stem (S-ZnO-NP) and callus (C-ZnO-NP) aqueous extracts,
respectively. XRD analysis confirmed the formation of hexagonal wurtzite structures
having an average grain size between 5 and 20 nm in diameter. FTIR spectra revealed
the presence of stretching vibrations of -O-H, C-H, C-N, C = O groups involved in
reduction and stabilization of nanoparticles. SEM images recognize the presence of
spongy, spherical, porous agglomerated nanoparticles. DLS analysis and zeta
potential values validated the stability of ZnO-NPs. The present investigation puts
light on the photocatalytic activity and biological (antioxidant, anti-
inflammatory, antidiabetic, antimicrobial, anticancerous) applications of ZnO-NPs.
The current study is an attempt to describe an effective, simple and eco-friendly
method of ZnO-NP synthesis and to evaluate its potential for various industrial and
medical applications.
AN - WOS:000532886200001
AU - Jayappa, M. D.
AU - Ramaiah, C. K.
AU - Kumar, M. A. P.
AU - Suresh, D.
AU - Prabhu, A.
AU - Devasya, R. P.
AU - Sheikh, S.
C6 - APR 2020
DA - AUG
DO - 10.1007/s13204-020-01382-2
IS - 8
PY - 2020
SN - 2190-5509
2190-5517
SP - 3057-3074
ST - Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro
grown callus of Mussaenda frondosa L.: characterization and their applications
T2 - APPLIED NANOSCIENCE
TI - Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro
grown callus of Mussaenda frondosa L.: characterization and their applications
VL - 10
ID - 6797
ER -
TY - JOUR
AB - Background: The field of medicine and synthetic drug development have
advanced rapidly over the past few decades. However, research on alternative
medicine, such as phytochemicals cannot be ignored. The main reason for prominent
curiosity about phytochemicals stems from the belief that the usage of natural
compounds are safer and have lesser detrimental side effects. Objective: The aim of
the present review was to discuss in detail several phytochemicals that have been
studied or are being studied in the context of various neurological disorders,
including depression, Alzheimer’s disease, Huntington’s disease and even
neuroinflammatory disorders, such as encephalitis. Methods: The potential roles of
phytochemicals in treating or managing symptoms associated with neurological
disorders have been included in this article. All data included in this paper have
been pooled from various databases, including Google Scholar, PubMed, Science
Direct, Springer, and Wiley Online Library. Results: Phytochemicals have been
widely studied for their therapeutic properties associated with neurological
disorders. Using various experimental techniques for both in vivo and in vitro
experiments, studies have shown that phytochemicals do have antioxidant, anti-
inflammatory and neuroprotective activities, which play major roles in the
treatment of neurological diseases. Conclusion: Even though there has been
compelling evidence of the therapeutic role of phytochemicals, further research is
still required to evaluate the safety and efficacy of these medicines. Using
previously published papers as the foundation for additional research, such as
preclinical studies and clinical trials, phytochemicals can become a safer
alternative to synthetic drugs for treating a spectrum of neurological diseases. ©
2023, Bentham Science Publishers. All rights reserved.
AU - Jayaraman, M.
AU - Dutta, P.
AU - Krishnan, S.
AU - Arora, K.
AU - Sivakumar, D.
AU - Raghavendran, H. R. B.
DB - Scopus
DO - 10.2174/1871527321666220701153926
IS - 9
KW - alternative medicine
cancer
medicinal plants
neuro-inflammatory disorders
neuro-psychiatric
neurological
Phytochemicals
alkaloid
amine oxidase (flavin containing) isoenzyme A
antioxidant
apigenin
ataxin 3
berberine
betulic acid
borneol
brain derived neurotrophic factor
calcitonin gene related peptide
camphor
cannabidiol
carotenoid
carvacrol
catalase
catechin
chalcone
citalopram
cocaine
corticosteroid
corticosterone
corticotropin
crocin
curcumin
cyclooxygenase 2
deutetrabenazine
diosgenin
dopamine
endothelin 1
Fas ligand
ferulic acid
flavonoid
fluoxetine
fluticasone propionate
furocoumarin
galanin
gallic acid
gibberellin
glutathione
herbaceous agent
interleukin 17
jatrorrhizine
lactulose
licochalcone A
liposome
luteolin
lycopene
magnolol
malathion
mangiferin
mitochondrial DNA
natural product
neuropeptide Y
noradrenalin
oleanolic acid
oxindole
palmatine
parkin
parthenolide
pentetrazole
phenytoin
phytochemical
phytol
phytosterol
piperine
plant extract
polyglutamine
polyphenol
prion protein
probenecid
prostaglandin E2
pyridine derivative
quercetin
quetiapine
resveratrol
risperidone
ropinirole
saponin
sesquiterpene
silver nanoparticle
stigmasterol
survival motor neuron protein
tachykinin
terpene
tetrabenazine
thymol
thymoquinone
transcription factor FOXP3
triptolide
ursolic acid
valproic acid
virulence factor
Acanthamoeba castellanii
Achillea
Acinetobacter baumannii
aconite
allopathy
Alphavirus
Alzheimer disease
amygdala
amyotrophic lateral sclerosis
Andrographis paniculata
anticonvulsant activity
antidepressant activity
anxiety
apoptosis
aromatherapy
Artemisia capillaris
Asteraceae
astrocyte
astrocytosis
ataxia
Bacopa monnieri
bacterium adherence
bioinformatics
black cumin
blood brain barrier
bradykinesia
brain depth stimulation
brain ischemia
Bupleurum
Caenorhabditis elegans
callus
cancer prevention
Candida albicans
Cannabis sativa
cell infiltration
cell proliferation
Centella asiatica
chorea
coculture
cognition
cognitive defect
Coptis chinensis
coriander
Crocus sativus
cytokine production
cytotoxicity
demyelinating disease
demyelination
depression
discriminant analysis
DNA damage
drug design
drug safety
electric shock
encephalitis
epigenetics
Escherichia coli
Euterpe oleracea
Fabaceae
fatigue
flower color
forced swim test
gene expression
genetic counseling
genotype
Ginkgo biloba
ginseng
gooseberry
Haemophilus influenzae
headache
hemiparesis
Heracleum
herbal medicine
hippocampus
human
Huntington chorea
Hypericaceae
hyperlipidemia
hypothalamus hypophysis adrenal system
hypoxia
in vitro study
insect vector
intellectual impairment
Lantana camara
lipid peroxidation
Listeria monocytogenes
liver microsome
locomotion
Magnolia officinalis
major depression
malignant neoplasm
medicinal plant
meningoencephalitis
mental disease
microglia
migraine
mitochondrial biogenesis
molecular docking
Moringa oleifera
multiple myeloma
multiple sclerosis
muscle atrophy
myelination
myocarditis
Neisseria meningitidis
nerve degeneration
nervousness
neurofibrillary tangle
neurologic disease
neuroprotection
neurotoxicity
nonhuman
Ocimum tenuiflorum
oligodendroglia
optic neuritis
orchitis
oxidative stress
paresthesia
Parkinson disease
parkinsonism
parotitis
Passiflora
phagocytosis
phonophobia
Phyllanthus emblica
phytochemistry
posttraumatic stress disorder
prevalence
Primula
prion disease
protein aggregation
protein misfolding
quality of life
remyelinization
Review
risk assessment
rosemary
schizophrenia
seizure
signal transduction
Solanum nigrum
spinal muscular atrophy
spinocerebellar degeneration
splicing defect
Streptococcus pneumoniae
Terminalia bellirica
Tinospora cordifolia
traumatic brain injury
trinucleotide repeat
upregulation
Veratrum
virus replication
watermelon
Withania somnifera
Wnt signaling
wound healing
zebra fish
M3 - Review
PY - 2023
SP - 1275-1301
ST - Emerging Promise of Phytochemicals in Ameliorating Neurological Disorders
T2 - CNS and Neurological Disorders - Drug Targets
TI - Emerging Promise of Phytochemicals in Ameliorating Neurological Disorders
85166393840&doi=10.2174%2f1871527321666220701153926&partnerID=40&md5=3a19c38a334210
7505b45ebea31b5049
VL - 22
ID - 4970
ER -
TY - JOUR
AB - DNA formulations provide the basis for safe and cost effective vaccine. Low
efficiency is often observed in the delivery of DNA vaccines. In order to assess a
new strategy for oral DNA vaccine formulation and delivery, plasmid encoding
hemagglutinin (HA) gene of avian influenza virus, A/Ck/Malaysia/5858/04 (H5N1)
(pcDNA3.1/H5) was formulated using green synthesis of sliver nanoparticles (AgNP)
with polyethylene glycol (PEG). AgNP were successfully synthesized uniformly
dispersed with size in the range of 4 to 18 nm with an average size of 11 nm.
Cytotoxicity of the prepared AgNP was investigated in vitro and in vivo using MCF-7
cells and cytokine expression, respectively. At the concentration of - 5 log
10AgNP, no cytotoxic effects were detected in MCF-7 cells with 9.5% cell death
compared to the control. One-day-old specific pathogen-free (SPF) chicks immunized
once by oral gavage with 10 μl of pcDNA3.1/H5 (200 ng/ml) nanoencapsulated with 40
μl AgNP (3.7 × 10 - 2 μg of Ag) showed no clinical manifestations. PCR successfully
detect the AgNP/H5 plasmid from the duodenum of the inoculated chicken as early as
1 h post-immunization. Immunization of chickens with AgNP/H5 enhanced both pro
inflammatory and Th1-like expressions, although no significant differences were
recorded in the chickens inoculated with AgNP, AgNP/pcDNA3.1 and the control. In
addition, serum samples collected from immunized chickens with AgNP/H5 showed
rapidly increasing antibody against H5 on day 14 after immunization. The highest
average antibody titres were detected on day 35 post-immunization at 51.2 ± 7.5.
AgNP/H5 also elicited both CD4+ and CD8+ T cells in the immunized chickens as early
as day 14 after immunization, at 7.5 ± 2.0 and 20 ± 1.9 percentage, respectively.
Hence, single oral administrations of AgNP/H5 led to induce both the antibody and
cell-mediated immune responses as well as enhanced cytokine production. © 2012
AU - Jazayeri, S. D.
AU - Ideris, A.
AU - Zakaria, Z.
AU - Shameli, K.
AU - Moeini, H.
AU - Omar, A. R.
DB - Scopus
DO - 10.1016/j.jconrel.2012.04.015
IS - 1
KW - Avian in uenza virus
Cytokine
Green synthesis AgNP
H5 DNA vaccine
HI antibody
T cell
Animals
Antibody Formation
Cell Line, Tumor
Cell Survival
Chickens
Cytokines
Hemagglutinin Glycoproteins, Influenza Virus
Humans
Influenza A Virus, H5N1 Subtype
Influenza in Birds
Influenza Vaccines
Nanoparticles
Silver
T-Lymphocytes
Vaccines, DNA
Antibodies
Cell death
Cytotoxicity
DNA
Drug therapy
Gene encoding
Immunization
Polyethylene glycols
Vaccines
Viruses
avian influenza vaccine
B cell activating factor
collagen type 6
DNA vaccine
gamma interferon
Influenza virus hemagglutinin
interleukin 10
interleukin 12p40
interleukin 15
interleukin 18
interleukin 1beta
interleukin 2
interleukin 4
interleukin 6
interleukin 8
macrogol
silver nanoparticle
Average size
Avian influenza
Cell-mediated immune
Clinical manifestation
Cost effective
Cytokine expression
Cytokine production
Cytotoxic effects
Green synthesis
In-vitro
MCF-7 cells
Oral administration
pcDNA3.1
Plasmid encoding
Serum samples
T cells
animal cell
animal experiment
animal tissue
article
CD4+ T lymphocyte
CD8+ T lymphocyte
cell death
cell strain MCF 7
cell viability
cellular immunity
chick
clinical feature
controlled study
cytokine production
drug cytotoxicity
duodenum
in vitro study
in vivo study
influenza vaccination
Influenza virus A H5N1
nanoencapsulation
nonhuman
nucleotide sequence
particle size
priority journal
protein expression
Th1 cell
zeta potential
Disease control
M3 - Article
PY - 2012
SP - 116-123
ST - Cytotoxicity and immunological responses following oral vaccination of
nanoencapsulated avian influenza virus H5 DNA vaccine with green synthesis silver
nanoparticles
TI - Cytotoxicity and immunological responses following oral vaccination of
nanoencapsulated avian influenza virus H5 DNA vaccine with green synthesis silver
nanoparticles
84862596004&doi=10.1016%2fj.jconrel.2012.04.015&partnerID=40&md5=c146693e2db1ad7f58
60035c1ffd9344
VL - 161
ID - 5714
ER -
TY - JOUR
AB - Background: Silver acetate is frequently used as an antimicrobial coating of
prosthetic vascular grafts. However, the effects of this coating on the early
inflammatory and angiogenic host tissue response still remain elusive. Therefore,
the aim of the present in vivo study was to analyze the biocompatibility and
vascularization of silver acetate-coated and uncoated vascular grafts during the
initial phase after implantation. Methods: Two different prosthetic vascular grafts
(ie, uncoated Dacron and silver acetate-coated Dacron Silver) were implanted into
the dorsal skinfold chamber of C57BL/6 mice (n = 8 per group) to study angiogenesis
and leukocytic inflammation at the implantation site by means of repetitive
intravital fluorescence microscopy over a 14-day period. At the end of the in vivo
experiments, collagen formation, apoptosis, and cell proliferation were analyzed in
the newly developed granulation tissue surrounding the implants by histology and
immunohistochemistry. Results: During the initial 14 days after implantation,
Dacron Silver exhibited an improved vascularization, as indicated by a
significantly increased functional capillary density compared with Dacron. This was
not associated with a stronger leukocytic inflammatory host tissue response to the
implants. Moreover, silver acetate coating did not affect collagen formation,
apoptosis, and cell proliferation at the implantation site. Conclusions: Silver
acetate coating of prosthetic vascular grafts improves their early vascularization
without inducing severe inflammatory side effects. Accordingly, this material
modification crucially contributes to an improved incorporation of the implants
into the host tissue, which may decrease the risk of vascular graft infection.
Copyright © 2013 by the Society for Vascular Surgery.
AU - Jeanmonod, P.
AU - Laschke, M. W.
AU - Gola, N.
AU - Von Heesen, M.
AU - Glanemann, M.
AU - Dold, S.
AU - Menger, M. D.
AU - Moussavian, M. R.
DB - Scopus
DO - 10.1016/j.jvs.2013.02.012
IS - 6
KW - Acetates
Animals
Arterial Occlusive Diseases
Blood Vessel Prosthesis
Follow-Up Studies
Inflammation
Mice
Mice, Inbred C57BL
Silver Compounds
Time Factors
acetate silver
antiinfective agent
collagen
dacron silver
unclassified drug
animal cell
animal experiment
animal tissue
apoptosis
article
biocompatibility
cell proliferation
collagen synthesis
dacron vascular prosthesis
hemodynamics
in vivo study
inflammation
intravital fluorescence microscopy
microscopy
mouse
nonhuman
priority journal
silver acetate coated dacron vascular prosthesis
vascularization
M3 - Article
PY - 2013
SP - 1637-1643
ST - Silver acetate coating promotes early vascularization of Dacron vascular
grafts without inducing host tissue inflammation
T2 - Journal of Vascular Surgery
TI - Silver acetate coating promotes early vascularization of Dacron vascular
grafts without inducing host tissue inflammation
84888327839&doi=10.1016%2fj.jvs.2013.02.012&partnerID=40&md5=671f7b4fc8c6afe5351671
81401d6ce4
VL - 58
ID - 5654
ER -
TY - JOUR
AB - Objectives In vascular surgery, the infection of prosthetic vascular grafts
represents a serious life-threatening complication. Due to the increasing
resistance of hospital micro-organisms to standard antibiotic therapies, maximum
effort should be put in the primary prevention of such infections. For this
purpose, grafts may be coated with different antibacterial silver formulations. In
the present study the different effects of silver acetate-coating and vaporized
metallic silver-coating on the vascularization and perigraft inflammation during
the initial phase after implantation of Intergard Silver (IS) and Silver Graft (SG)
were compared. Methods Silver acetate-coated IS and vaporized metallic silver-
coated SG were implanted into the dorsal skinfold chamber of C57BL/6 mice (n = 8
per group) to study angiogenesis and leukocyte inflammation at the implantation
site by means of repetitive intravital fluorescence microscopy over a 14-day
period. At the end of the in vivo experiments, apoptosis and cell proliferation in
the newly developed granulation tissue surrounding the implants was analyzed by
immunohistochemistry. Results IS exhibited an improved vascularization, resulting
in a significantly higher functional capillary density when compared to SG.
Moreover, the leukocyte inflammatory response to IS was less pronounced, as
indicated by a reduced number of adherent leukocytes in perigraft venules. This was
associated with a higher proliferative activity of the granulation tissue
incorporating the IS when compared to SG. The numbers of apoptotic cells in the
perigraft tissue were low and did not differ between the two groups. Conclusion
Silver acetate-coated IS exhibits an improved vascularization and reduced perigraft
inflammation during the first 14 days after implantation when compared to vaporized
metallic silver-coated SG. This may contribute to reducing the risk of early
perigraft seroma formation and subsequent infection.© 2014 European Society for
Vascular Surgery. Published by Elsevier Ltd. All rights reserved.
AU - Jeanmonod, P.
AU - Laschke, M. W.
AU - Gola, N.
AU - Von Heesen, M.
AU - Glanemann, M.
AU - Menger, M. D.
AU - Moussavian, M. R.
DB - Scopus
DO - 10.1016/j.ejvs.2014.03.006
IS - 6
KW - Angiogenesis
Dacron grafts
Dorsal skinfold chamber
Inflammation
Silver acetate
Vaporized silver
Vascularization
Acetates
Animals
Apoptosis
Blood Vessel Prosthesis Implantation
Cell Proliferation
Mice
Mice, Inbred C57BL
Silver Compounds
Time Factors
acetate silver
silver
unclassified drug
vaporized metallic silver
angiogenesis
animal experiment
apoptosis
article
blood vessel graft
blood vessel prosthesis
capillary density
cell proliferation
granulation tissue
implant
implantation
in vivo study
inflammation
intravital fluorescence microscopy
leukocyte
mouse
nonhuman
priority journal
skinfold
tissue reaction
vascularization
venule
M3 - Article
PY - 2014
SP - 680-688
ST - Early host tissue response to different types of vascular prostheses coated
with silver acetate or vaporized metallic silver
T2 - European Journal of Vascular and Endovascular Surgery
TI - Early host tissue response to different types of vascular prostheses coated
with silver acetate or vaporized metallic silver
84901619465&doi=10.1016%2fj.ejvs.2014.03.006&partnerID=40&md5=83247fdc565c3637e4b1e
b4e5e190781
VL - 47
ID - 5578
ER -
TY - JOUR
AB - Inhalation of engineered nanoparticles (NP) poses a still unknown risk.
Individuals with chronic lung diseases are expected to be more vulnerable to
adverse effects of NP than normal subjects, due to altered respiratory structures
and functions. Realistic and dose-controlled aerosol exposures were performed using
the deposition chamber NACIVT. Well-differentiated normal and cystic fibrosis (CF)
human bronchial epithelia (HBE) with established air-liquid interface and the human
bronchial epithelial cell line BEAS-2B were exposed to spark-generated silver and
carbon nanoaerosols (20 nm diameter) at three different doses. Necrotic and
apoptotic cell death, pro-inflammatory response, epithelial function and morphology
were assessed within 24 h after aerosol exposure. NP exposure resulted in
significantly higher necrosis in CF than normal HBE and BEAS-2B cells. Before and
after NP treatment, CF HBE had higher caspase-3 activity and secreted more IL-6 and
MCP-1 than normal HBE. Differentiated HBE had higher baseline secretion of IL-8 and
less caspase-3 activity and MCP-1 secretion compared to BEAS-2B cells. These
biomarkers increased moderately in response to NP exposure, except for MCP-1, which
was reduced in HBE after AgNP treatment. No functional and structural alterations
of the epithelia were observed in response to NP exposure. Significant differences
between cell models suggest that more than one and fully differentiated HBE should
be used in future toxicity studies of NP in vitro.Our findings support
epidemiologic evidence that subjects with chronic airway diseases are more
vulnerable to adverse effects of particulate air pollution. Thus, this sub-
population needs to be included in nano-toxicity studies. © 2015 Informa UK Ltd.
All rights reserved: reproduction in whole or part not permitted.
AU - Jeannet, N.
AU - Fierz, M.
AU - Schneider, S.
AU - Künzi, L.
AU - Baumlin, N.
AU - Salathe, M.
AU - Burtscher, H.
AU - Geiser, M.
DB - Scopus
DO - 10.3109/17435390.2015.1049233
IS - 3
KW - Air'liquid interface
airways
cell death
NACIVT
pro-inflammatory cytokines
Aerosols
Carbon
Cell Death
Cell Proliferation
Cells, Cultured
Cystic Fibrosis
Epithelial Cells
Humans
Nanoparticles
Particulate Matter
Respiratory Mucosa
Silver
carbon nanoparticle
caspase 3
interleukin 6
interleukin 8
silver nanoparticle
aerosol
autacoid
carbon
nanoparticle
particulate matter
silver
acute toxicity
air pollution
Article
cell culture
ciliated epithelium
dose response
enzyme activity
epithelial lining fluid
human
human cell
lung fibrosis
neutrophil
nonhuman
priority journal
cell proliferation
chemistry
cystic fibrosis
cytology
drug effects
epithelium cell
metabolism
pathology
physiology
respiratory mucosa
toxicity
M3 - Article
PY - 2016
SP - 279-291
ST - Acute toxicity of silver and carbon nanoaerosols to normal and cystic
fibrosis human bronchial epithelial cells
T2 - Nanotoxicology
TI - Acute toxicity of silver and carbon nanoaerosols to normal and cystic
84959268343&doi=10.3109%2f17435390.2015.1049233&partnerID=40&md5=0f76fc6b74d89bbbfc
844637fcd58dd6
VL - 10
ID - 5483
ER -
TY - JOUR
AB - Inhalation of engineered nanoparticles (NP) poses a still unknown risk.
Individuals with chronic lung diseases are expected to be more vulnerable to
adverse effects of NP than normal subjects, due to altered respiratory structures
and functions. Realistic and dose-controlled aerosol exposures were performed using
the deposition chamber NACIVT. Well-differentiated normal and cystic fibrosis (CF)
human bronchial epithelia (HBE) with established air-liquid interface and the human
bronchial epithelial cell line BEAS-2B were exposed to spark-generated silver and
carbon nanoaerosols (20nm diameter) at three different doses. Necrotic and
apoptotic cell death, pro-inflammatory response, epithelial function and morphology
were assessed within 24h after aerosol exposure. NP exposure resulted in
significantly higher necrosis in CF than normal HBE and BEAS-2B cells. Before and
after NP treatment, CF HBE had higher caspase-3 activity and secreted more IL-6 and
MCP-1 than normal HBE. Differentiated HBE had higher baseline secretion of IL-8 and
less caspase-3 activity and MCP-1 secretion compared to BEAS-2B cells. These
biomarkers increased moderately in response to NP exposure, except for MCP-1, which
was reduced in HBE after AgNP treatment. No functional and structural alterations
of the epithelia were observed in response to NP exposure. Significant differences
between cell models suggest that more than one and fully differentiated HBE should
be used in future toxicity studies of NP in vitro.Our findings support
epidemiologic evidence that subjects with chronic airway diseases are more
vulnerable to adverse effects of particulate air pollution. Thus, this sub-
population needs to be included in nano-toxicity studies.
AN - WOS:000371819000002
AU - Jeannet, N.
AU - Fierz, M.
AU - Schneider, S.
AU - Kunzi, L.
AU - Baumlin, N.
AU - Salathe, M.
AU - Burtscher, H.
AU - Geiser, M.
DA - MAR 15
DO - 10.3109/17435390.2015.1049233
IS - 3
PY - 2016
SN - 1743-5390
1743-5404
SP - 279-291
ST - Acute toxicity of silver and carbon nanoaerosols to normal and cystic
T2 - NANOTOXICOLOGY
TI - Acute toxicity of silver and carbon nanoaerosols to normal and cystic
VL - 10
ID - 6182
ER -
TY - JOUR
AB - An ongoing need for new cancer therapeutics exists, especially ones that
specifically home and target triple-negative breast cancer. Because triple-negative
breast cancer express low or are devoid of estrogen, progesterone, or Her2/Neu
receptors, another target must be used for advanced drug delivery strategies. Here,
we engineered a nanodrug delivery system consisting of silver-coated gold nanorods
(AuNR/Ag) targeting epithelial cell adhesion/activating molecule (EpCAM) and loaded
with doxorubicin. This nanodrug system, AuNR/Ag/Dox-EpCAM, was found to
specifically target EpCAM-expressing tumors compared to low EpCAM-expressing
tumors. Namely, the nanodrug had an effective dose (ED50) of 3 mu M in inhibiting
4T1 cell viability and an ED50 of 110 mu M for MDA-MD-231 cells. Flow cytometry
data indicated that 4T1 cells, on average, express two orders of magnitude more
EpCAM than MDA-MD-231 cells, which correlates with our ED50 findings. Moreover, due
to the silver coating, the AuNR/Ag can be detected simultaneously by surface-
enhanced Raman spectroscopy and photoacoustic microscopy. Analysis by these imaging
detection techniques as well as by inductively coupled plasma mass spectrometry
showed that the targeted nanodrug system was taken up by EpCAM-expressing cells and
tumors at significantly higher rates than untargeted nanoparticles (p < 0.05).
Thus, this approach establishes a plasmonically active nanodrug theranostic for
triple-negative breast cancer and, potentially, a delivery platform with improved
multimodal imaging capability for other clinically relevant chemotherapeutics with
dose-limiting toxicities, such as platinum-based or taxane-based therapies.
AN - WOS:000413628300001
AU - Jenkins, S. V.
AU - Nima, Z. A.
AU - Vang, K. B.
AU - Kannarpady, G.
AU - Nedosekin, D. A.
AU - Zharov, V. P.
AU - Griffin, R. J.
AU - Biris, A. S.
AU - Dings, R. P. M.
C7 - 27
DA - SEP 1
DO - 10.1038/s41698-017-0030-1
PY - 2017
SN - 2397-768X
ST - Triple-negative breast cancer targeting and killing by EpCAM-directed,
plasmonically active nanodrug systems
T2 - NPJ PRECISION ONCOLOGY
TI - Triple-negative breast cancer targeting and killing by EpCAM-directed,
plasmonically active nanodrug systems
VL - 1
ID - 6739
ER -
TY - JOUR
AB - Since its first use in 1917, sulphur mustard (SM) has been used virtually
exclusively as a weapon of war. SM is a volatile liquid that damages any tissue it
contacts as a vapour or liquid. SM primarily damages the skin, eyes and lungs
producing massive inflammation culminating in the characteristic blistering of the
skin which classifies SM as a vesicant. Several mechanisms of action at the
cellular level have been proposed for SM, but none has ever been convincingly
linked to the production of blisters or vesication. First aid for those
contaminated with liquid SM consists of the rapid removal (within a few minutes) of
liquid from the surface of the skin, as once penetrated into the stratum corneum it
is very difficult to remove. In the absence of a mechanistically based specific
therapy, SM skin injury is normally treated in a similar way to thermal and
chemical burns, which it resembles pathologically. Effective therapy consist of
treating the inflammation and where necessary removal of the dead eschar to
facilitate healing. Post surgical care comprises the use of one of a number of
available dressings used in thermal burn care and antibiotic creams should
infection be present. © 2013 Elsevier B.V.
AU - Jenner, J.
AU - Graham, S. J.
DB - Scopus
DO - 10.1016/j.cbi.2013.10.015
IS - 3
KW - Adjunct therapies
Anti-inflammatories
Chemical weapons
Dressings
Sulphur mustard
Surgical treatment
Animals
Bandages
Chemical Warfare Agents
Erythema
Humans
Mustard Gas
Skin
Skin Diseases
acetylcysteine
chlorpromazine
dexamethasone
diclofenac
dimercaprol
doxycycline
hydrocortisone
indometacin
methylprednisolone
mustard gas
nicotinamide
octyl homovanillamide
olvanil
proteinase inhibitor
retro olvanil
silibinin
unclassified drug
adhesive dressing
alginate dressing
article
cytotoxicity
debridement
erbium YAG laser
foam dressing
human
hydrocolloid dressing
hydrogel dressing
nonhuman
pathology
silver donating dressing
skin decontamination
skin incision
skin injury
skin surgery
supplementation
vacuum assisted closure
wound dressing
wound healing
M3 - Article
PY - 2013
SP - 491-495
ST - Treatment of sulphur mustard skin injury
TI - Treatment of sulphur mustard skin injury
84890127101&doi=10.1016%2fj.cbi.2013.10.015&partnerID=40&md5=de15eb3bef35b60833640b
59bf6207e5
VL - 206
ID - 5563
ER -
TY - JOUR
AB - The aim of the present study was to systematically review studies
investigating antibacterial implant abutment surfaces or coatings, which may
suppress bacterial growth to prevent plaque-induced peri-implant inflammatory
disease. Data were collected after identification of case, assay/laboratory
procedure, predicate/reference standard and outcome (CAPO). Seven hundred and
twenty (720) records were identified through data base searching. After screening
nine publications fulfilled inclusion criteria and were included. The following
surfaces/coatings showed antibacterial properties: Electrochemical surface
modification of titanium by the anodic spark deposition tech-nique; doxycycline
coating by cathodic polarization; silver coating by DC plasma sputter; titanium
nitride; zirconium nitride and microwave assistant nano silver coating. Since the
current state of the literature is rather descriptive, a meta-analysis was not
performed. While several abutment coatings showed to have antibacterial capacity,
some of them also influenced the behavior of investigated human cells. None of the
studies investigated the long-term effect of surface modifications. Since surface
changes are the main contributing factor in the development of antibacterial
effects, the biodegradation behavior must be characterized to understand its
durability. To date there is no effective structure, material or strategy to avoid
periimplant inflammation used as clinical routine. Furthermore, clinical studies
are scarce. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Jennes, M. E.
AU - Naumann, M.
AU - Peroz, S.
AU - Beuer, F.
AU - Schmidt, F.
C7 - 1350
DB - Scopus
IS - 11
KW - Abutment
Antibacterial coating
Implant
Peri-implantitis
Prevention
silver nanoparticle
Article
bacterial viability
biocompatibility
bone mineralization
bone regeneration
cytotoxicity
dispersity
electrochemical analysis
electrospinning
fracture healing
inflammation
intestine metaplasia
macrophage
MTT assay
nonhuman
osseointegration
periimplantitis
periodontitis
phase contrast microscopy
Streptococcus mutans
Streptococcus salivarius
surface property
systematic review
tooth disease
tooth plaque
M3 - Article
PY - 2021
ST - Antibacterial effects of modified implant abutment surfaces for the
prevention of peri-implantitis—a systematic review
T2 - Antibiotics
TI - Antibacterial effects of modified implant abutment surfaces for the
prevention of peri-implantitis—a systematic review
85118947561&doi=10.3390%2fantibiotics10111350&partnerID=40&md5=e8d9c7b94a70ecf1bcaa
3838ed9ca8e9
VL - 10
ID - 5224
ER -
TY - JOUR
AB - The measurement of nanoparticles (NPs) in a biological matrix is essential in
various toxicity studies. However, the current knowledge has limitations in
differentiating particulate and ionic forms and further identification of their
biotransformation. Herein, we evaluate the biotransformation and differential lung
clearance kinetics of particulate and ionic forms using PEGylated silver NPs (AgNP-
PEGs; 47.51 nm) and PEGylated gold NPs (AuNP-PEGs; 11.76 nm). At 0, 3, and 6 h and
1, 3, 7, and 14 days after a single pharyngeal aspiration in mice at 25 μg/mouse,
half of the lung is digested by proteinase K (PK) to separate particulates and
ions, and the other half is subjected to the acid digestion method for comparison.
The quantitative and qualitative evaluation of lung clearance kinetics suggests
that AgNP-PEGs are quickly dissolved and transformed into insoluble silver sulfide
(Ag2S), which shows a fast-clearing early phase (0 −6 h; particle T1/2: 4.8 h) and
slow-clearing late phase (1 −14 days; particle T1/2: 13.20 days). In contrast,
AuNP-PEGs were scarcely cleared or biotransformed in the lungs for 14 days. The
lung clearance kinetics of AgNPs and biotransformation shown in this study can be
informed by the PK digestion method and cannot be obtained using the acid digestion
method. © 2023 Elsevier B.V.
AU - Jeon, S.
AU - Lee, W. S.
AU - Song, K. S.
AU - Jeong, J.
AU - Lee, S.
AU - Kim, S.
AU - Kim, G.
AU - Kim, J. S.
AU - Cho, W. S.
C7 - 131223
DB - Scopus
DO - 10.1016/j.jhazmat.2023.131223
KW - Dissolution
Inhalation
Lung burden
Particokinetics
Toxicokinetics
Animals
Biotransformation
Ions
Lung
Metal Nanoparticles
Mice
Particle Size
Silver
Bioconversion
Biological organs
Kinetics
Particles (particulate matter)
Silver compounds
Sulfur compounds
dissolved oxygen
ion
isoflurane
proteinase K
silver nanoparticle
silver sulfide
sulfide
unclassified drug
macrogol
metal nanoparticle
silver
Acid digestion methods
Ionic forms
Particle kinetics
Particokinetic
Particulates
Pegylated
Proteinase K
acid digestion
biotransformation
nanoparticle
reaction kinetics
toxicity
animal experiment
animal model
animal tissue
Article
blood vessel permeability
comparative study
controlled study
dosimetry
female
hydrodynamics
inflammatory cell
innate immunity
lung burden
lung clearance
lung parenchyma
macrophage
morphological trait
mouse
neutrophil
nonhuman
particle size
pathogenesis
PEGylation
phagolysosome
pneumonia
animal
lung
metabolism
Mammals
M3 - Article
PY - 2023
ST - Differential particle and ion kinetics of silver nanoparticles in the lungs
and biotransformation to insoluble silver sulfide
TI - Differential particle and ion kinetics of silver nanoparticles in the lungs
and biotransformation to insoluble silver sulfide
85150437173&doi=10.1016%2fj.jhazmat.2023.131223&partnerID=40&md5=a575d0371db1cffc38
b978b39e32a9a7
VL - 452
ID - 5042
ER -
TY - JOUR
AB - The increased interest in nanomedicine and its applicability for a wide range
of biological functions demands the search for raw materials to create
nanomaterials. Recent trends have focused on the use of green chemistry to
synthesize metal and metal-oxide nanoparticles. Bioactive chemicals have been found
in a variety of marine organisms, including invertebrates, marine mammals, fish,
algae, plankton, fungi, and bacteria. These marine-derived active chemicals have
been widely used for various biological properties. Marine-derived materials,
either whole extracts or pure components, are employed in the synthesis of
nanoparticles due to their ease of availability, low cost of production,
biocompatibility, and low cytotoxicity toward eukaryotic cells. These marine-
derived nanomaterials have been employed to treat infectious diseases caused by
bacteria, fungi, and viruses as well as treat non-infectious diseases, such as
tumors, cancer, inflammatory responses, and diabetes, and support wound healing.
Furthermore, several polymeric materials derived from the marine, such as chitosan
and alginate, are exploited as nanocarriers in drug delivery. Moreover, a variety
of pure bioactive compounds have been loaded onto polymeric nanocarriers and
employed to treat infectious and non-infectious diseases. The current review is
focused on a thorough overview of nanoparticle synthesis and its biological
applications made from their entire extracts or pure chemicals derived from marine
sources.
AN - WOS:000845688600001
AU - Jeong, G. J.
AU - Khan, S.
AU - Tabassum, N.
AU - Khan, F.
AU - Kim, Y. M.
C7 - 527
DA - AUG
DO - 10.3390/md20080527
IS - 8
PY - 2022
SN - 1660-3397
ST - Marine-Bioinspired Nanoparticles as Potential Drugs for Multiple Biological
Roles
T2 - MARINE DRUGS
TI - Marine-Bioinspired Nanoparticles as Potential Drugs for Multiple Biological
Roles
VL - 20
ID - 6650
ER -
TY - JOUR
AB - Overexpression of pro-inflammatory cytokines, including tumour necrosis
factor alpha (TNF alpha), has been implicated in the pathogenesis of anaemia of
inflammation. TNF alpha suppresses erythroid colony formation via both direct and
indirect effects on haematopoietic progenitors, often involving activation of
nuclear factor (NF-kappa B signalling resulting in downregulation of transcription
factors critical for erythropoiesis. There is a dearth of effective and safe
therapies for many patients with inflammatory anaemia. Resveratrol is a flavanol
found in red wine grapes that possesses potent anti-inflammatory properties, but
studies of its impact on human erythropoiesis have proven contradictory. We
investigated whether resveratrol ameliorates TNF alpha-mediated suppression of
erythropoiesis in human CD34(+) haematopoietic progenitors. We found that
resveratrol partially reverses the erythroid suppressive effects of TNF alpha,
leading to significant recovery in burst forming unit-erythroid colony formation in
human CD34(+) cells. CD34(+) cells preincubated with resveratrol for 72 h in the
presence of TNF alpha inhibited NF-kappa B activation via decreased NF-kappa B
nuclear localization without altering total NF-kappa B protein levels and
independent of I kappa B degradation. Resveratrol also significantly restored the
baseline expression of erythroid transcription factors NFE2 and the GATA1/GATA2
ratio in CD34(+) cells treated with TNF alpha. In conclusion, resveratrol may
inhibit TNF alpha-mediated NF-kappa B activation and promote erythropoiesis in
primary human CD34(+) cells.
AN - WOS:000294919700009
AU - Jeong, J. Y.
AU - Silver, M.
AU - Parnes, A.
AU - Nikiforow, S.
AU - Berliner, N.
AU - Vanasse, G. J.
DA - OCT
DO - 10.1111/j.1365-2141.2011.08800.x
IS - 1
PY - 2011
SN - 0007-1048
SP - 93-101
ST - Resveratrol ameliorates TNF alpha-mediated suppression of erythropoiesis in
human CD34(+) cells via modulation of NF-kappa B signalling
T2 - BRITISH JOURNAL OF HAEMATOLOGY
TI - Resveratrol ameliorates TNF alpha-mediated suppression of erythropoiesis in
human CD34(+) cells via modulation of NF-kappa B signalling
VL - 155
ID - 6547
ER -
TY - JOUR
AB - ObjectiveNeurodegeneration induced by inflammatory stress in multiple
sclerosis (MS) leads to long-term neurological disabilities that are not amenable
to current immunomodulatory therapies. Methods and ResultsHere, we report that
neuronal downregulation of Splicing factor 3b subunit 2 (SF3B2), a component of U2
small nuclear ribonucleoprotein (snRNP), preserves retinal ganglion cell (RGC)
survival and axonal integrity in experimental autoimmune encephalomyelitis (EAE)-
induced mice. By employing an in vitro system recapitulating the inflammatory
environment of MS lesion, we show that when SF3B2 levels are downregulated, cell
viability and axon integrity are preserved in cortical neurons against inflammatory
toxicity. Notably, knockdown of SF3B2 suppresses the expression of injury-response
and necroptosis genes and prevents activation of Sterile Alpha and TIR Motif
Containing 1 (Sarm1), a key enzyme that mediates programmed axon degeneration.
InterpretationTogether, these findings suggest that the downregulation of SF3B2 is
a novel potential therapeutic target to prevent secondary neurodegeneration in MS.
AN - WOS:000904579400001
AU - Jeong, Y. E.
AU - Rajbhandari, L.
AU - Kim, B. W.
AU - Venkatesan, A.
AU - Hoke, A.
C6 - DEC 2022
DA - FEB
DO - 10.1002/acn3.51717
IS - 2
PY - 2023
SN - 2328-9503
SP - 246-265
ST - Downregulation of SF3B2 protects CNS neurons in models of multiple sclerosis
T2 - ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
TI - Downregulation of SF3B2 protects CNS neurons in models of multiple sclerosis
VL - 10
ID - 6698
ER -
TY - JOUR
AB - Cratoxylum formosum Dyer is a medicinal plant widely found in Asia and
commonly consumed for food and folk medicine. It is rich in phenolic compounds. The
present study utilized water crude extract of C. formosum leaves to synthesize zinc
oxide nanoparticles (ZnO NPs) by green synthesis. The synthesized ZnO NPs with the
average electronic band gap similar to 3 eV were obtained and found to either have
spherical shape or sheet-like structures depending on synthesis process and
concentration of crude extract. Higher concentration of C. formosum extract also
eliminates impurity of Zn(OH)(2) during the synthesis. Results from an agar disk
diffusion assay demonstrated that all synthesized ZnO samples inhibited growth of
Gram-positive bacteria, Bacillus subtilis and Staphylococcus epidermidis and Gram-
negative bacterium, Escherichia coli. Furthermore, all synthesized ZnO demonstrated
potent anti-cancer activity against non-melanoma skin cancer cells (A431) and the
intermediary of cancerous keratinocytes (HaCaT) without affecting normal cell lines
(Vero). In addition, we observed that the ZnO nanosheet offered stronger
cytotoxicity effects against A431 than spherical shaped ZnO particles. Analysis of
RNA-sequencing data revealed that synthesized ZnO nanosheets altered the number of
genes in pathways involved in cancer and MAPK signaling pathways in A431 cells.
Several isoforms of metallothionein transcripts were upregulated including
transcripts involved in inflammatory responses whereas transcripts promoted cell
proliferation and apoptosis were downregulated. Therefore, these studies firstly
reported potential usage of the green-synthesized ZnO nanosheets from C. formosum
extract for development of antibacterial substances or anticancer drugs.
AN - WOS:000582697900057
AU - Jevapatarakul, D.
AU - T-Thienprasert, J.
AU - Payungporn, S.
AU - Chavalit, T.
AU - Khamwut, A.
AU - T-Thienprasert, N. P.
C7 - 110552
DA - OCT
DO - 10.1016/j.biopha.2020.110552
PY - 2020
SN - 0753-3322
1950-6007
ST - Utilization of Cratoxylum formosum crude extract for synthesis of ZnO
nanosheets: Characterization, biological activities and effects on gene expression
of nonmelanoma skin cancer cell
T2 - BIOMEDICINE & PHARMACOTHERAPY
TI - Utilization of Cratoxylum formosum crude extract for synthesis of ZnO
nanosheets: Characterization, biological activities and effects on gene expression
of nonmelanoma skin cancer cell
VL - 130
ID - 6520
ER -
TY - JOUR
AB - As the applications and environmental release of silver ions and
nanoparticles are increasing, increasing human exposure to these pollutants has
become an emerging health concern. The impeding effects of such pollutants on
susceptible populations are severely under-studied. Here, we demonstrate that
silver nanoparticles (Ag NPs), at a dose that causes no general toxicity in normal
mice, promotes the progression of fatty liver disease from steatosis to
steatohepatitis only in overweight mice. Exposure to Ag+ ions induces the same
effects in overweight mice. Ag NPs rather than Ag ions cause this disease
progression based on our findings that Ag+ ions are partly reduced to Ag NPs in
fatty livers, and the toxic effect is correlated with the liver dose of Ag NPs, not
Ag+ ions. Furthermore, the Ag NP-induced pro-inflammatory activation of Kupffer
cells in the liver, enhancement of hepatic inflammation, and suppression of fatty
acid oxidation are identified as key factors in the underlying mechanisms.
AN - WOS:000407987400050
AU - Jia, J. B.
AU - Li, F. F.
AU - Zhou, H. Y.
AU - Bai, Y. H.
AU - Liu, S. J.
AU - Jiang, Y. G.
AU - Jiang, G. B.
AU - Yan, B.
DA - AUG 15
DO - 10.1021/acs.est.7b02752
IS - 16
PY - 2017
SN - 0013-936X
1520-5851
SP - 9334-9343
ST - Oral Exposure to Silver Nanoparticles or Silver Ions May Aggravate Fatty
Liver Disease in Overweight Mice
T2 - ENVIRONMENTAL SCIENCE & TECHNOLOGY
TI - Oral Exposure to Silver Nanoparticles or Silver Ions May Aggravate Fatty
VL - 51
ID - 6117
ER -
TY - JOUR
AB - Systemic low-grade inflammation and imbalance of gut microbiota are important
risk factors promoting the progression of obesity-related metabolic disorders. This
provides potential pharmacological and nutritional targets for the management of
obesity and obesity-related disorders. Here, we evaluated the modulatory effects of
nanosilver on obesity-related systemic low-grade inflammation and gut microbial
dysbiosis. C57BL/6J mice were fed with normal diet (ND) or high-fat diet (HFD) for
6 months, with/without nanosilver supplementation in drinking water. Nanosilver
administration showed little systemic toxicity and did not affect the progression
of obesity but mitigated the obesity-related systemic low-grade inflammation in
obese mice. Such mitigation of systemic low-grade inflammation was specifically
mediated by reducing the inflammatory status of epididymal visceral white adipose
tissue (eWAT). Nanosilver treatments increased the diversity of gut microbial
communities and markedly recovered the relative abundance of Verrucomicrobia,
Epsilonbacteraeota, Actinobacteria, and Deferribacteres, without altering the
proportion of Bacteroidetes or Firmicutes. The beneficial effects of nanosilver in
obese mice were in association with an increase in Akkermansia but a decrease in
Parasutterella at the genus level. This study suggested a potential application of
nanosilver in reducing the health risks of obesity.
AN - WOS:000630168200051
AU - Jia, J. B.
AU - Zhang, W.
AU - Wu, Y. X.
AU - Zhang, X. L.
AU - Li, C. J.
AU - Wang, J. Z.
AU - Yan, B.
C6 - FEB 2021
DA - MAR 15
DO - 10.1021/acsabm.0c01560
IS - 3
PY - 2021
SN - 2576-6422
SP - 2570-2582
ST - Mitigation of Obesity-Related Systemic Low-Grade Inflammation and Gut
Microbial Dysbiosis in Mice with Nanosilver Supplement
T2 - ACS APPLIED BIO MATERIALS
TI - Mitigation of Obesity-Related Systemic Low-Grade Inflammation and Gut
VL - 4
ID - 6759
ER -
TY - JOUR
AB - As the applications and environmental release of silver ions and
nanoparticles are increasing, increasing human exposure to these pollutants has
become an emerging health concern. The impeding effects of such pollutants on
susceptible populations are severely under-studied. Here, we demonstrate that
silver nanoparticles (Ag NPs), at a dose that causes no general toxicity in normal
mice, promotes the progression of fatty liver disease from steatosis to
steatohepatitis only in overweight mice. Exposure to Ag+ ions induces the same
effects in overweight mice. Ag NPs rather than Ag+ ions cause this disease
progression based on our findings that Ag+ ions are partly reduced to Ag NPs in
fatty livers, and the toxic effect is correlated with the liver dose of Ag NPs, not
Ag+ ions. Furthermore, the Ag NP-induced pro-inflammatory activation of Kupffer
cells in the liver, enhancement of hepatic inflammation, and suppression of fatty
acid oxidation are identified as key factors in the underlying mechanisms. © 2017
AU - Jia, J.
AU - Li, F.
AU - Zhou, H.
AU - Bai, Y.
AU - Liu, S.
AU - Jiang, Y.
AU - Jiang, G.
AU - Yan, B.
DB - Scopus
DO - 10.1021/acs.est.7b02752
IS - 16
KW - Animals
Ions
Liver Diseases
Metal Nanoparticles
Mice
Non-alcoholic Fatty Liver Disease
Overweight
Oxidative Stress
Silver
Mus
Diseases
Fatty acids
Liver
Mammals
Metal ions
Metal nanoparticles
Nanoparticles
Pathology
Pollution
Toxicity
cholesterol
high density lipoprotein
interleukin 10
interleukin 6
low density lipoprotein
metal ion
peroxisome proliferator activated receptor delta
silver nanoparticle
ion
metal nanoparticle
silver
Disease progression
Environmental release
Fatty acid oxidation
Fatty liver disease
Health concerns
Susceptible population
cation
disease
experimental study
fatty acid
nanoparticle
oxidation
pollution exposure
rodent
toxicity
abdominal fat
animal model
animal tissue
Article
cholesterol blood level
controlled study
cytokine production
disease course
down regulation
fatty acid oxidation
fatty liver
hepatitis
lipid blood level
male
mouse
nonalcoholic fatty liver
nonhuman
obesity
pH
stomach juice
upregulation
weight gain
animal
chemically induced
liver disease
oxidative stress
M3 - Article
PY - 2017
SP - 9334-9343
ST - Oral Exposure to Silver Nanoparticles or Silver Ions May Aggravate Fatty
T2 - Environmental Science and Technology
TI - Oral Exposure to Silver Nanoparticles or Silver Ions May Aggravate Fatty
85027447293&doi=10.1021%2facs.est.7b02752&partnerID=40&md5=4c6ee62d93fcad3a27685072
09bf535e
VL - 51
ID - 5463
ER -
TY - JOUR
AB - Systemic low-grade inflammation and imbalance of gut microbiota are important
risk factors promoting the progression of obesity-related metabolic disorders. This
provides potential pharmacological and nutritional targets for the management of
obesity and obesity-related disorders. Here, we evaluated the modulatory effects of
nanosilver on obesity-related systemic low-grade inflammation and gut microbial
dysbiosis. C57BL/6J mice were fed with normal diet (ND) or high-fat diet (HFD) for
6 months, with/without nanosilver supplementation in drinking water. Nanosilver
administration showed little systemic toxicity and did not affect the progression
of obesity but mitigated the obesity-related systemic low-grade inflammation in
obese mice. Such mitigation of systemic low-grade inflammation was specifically
mediated by reducing the inflammatory status of epididymal visceral white adipose
tissue (eWAT). Nanosilver treatments increased the diversity of gut microbial
communities and markedly recovered the relative abundance of Verrucomicrobia,
Epsilonbacteraeota, Actinobacteria, and Deferribacteres, without altering the
proportion of Bacteroidetes or Firmicutes. The beneficial effects of nanosilver in
obese mice were in association with an increase in Akkermansia but a decrease in
Parasutterella at the genus level. This study suggested a potential application of
nanosilver in reducing the health risks of obesity. © 2021 American Chemical
Society. All rights reserved.
AU - Jia, J.
AU - Zhang, W.
AU - Wu, Y.
AU - Zhang, X.
AU - Li, C.
AU - Wang, J.
AU - Yan, B.
DB - Scopus
DO - 10.1021/acsabm.0c01560
IS - 3
KW - eWAT inflammation
gut microbiota
healthcare
low-grade inflammation
nanosilver
obesity
Animals
Diet
Dysbiosis
Escherichia coli
Inflammation
Male
Materials Testing
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Particle Size
Silver
Health risks
Mammals
Pathology
Potable water
antiinfective agent
biomaterial
metal nanoparticle
silver
Actinobacteria
Beneficial effects
Gut microbiota
Metabolic disorders
Microbial communities
Relative abundance
Systemic toxicities
White adipose tissues
animal
C57BL mouse
chemistry
diet
drug effect
dysbiosis
inflammation
intestine flora
male
materials testing
mouse
particle size
Nutrition
M3 - Article
PY - 2021
SP - 2570-2582
ST - Mitigation of Obesity-Related Systemic Low-Grade Inflammation and Gut
T2 - ACS Applied Bio Materials
TI - Mitigation of Obesity-Related Systemic Low-Grade Inflammation and Gut
85101747243&doi=10.1021%2facsabm.0c01560&partnerID=40&md5=4581c70ff5b89e1b96a046b9b
79929d2
VL - 4
ID - 5186
ER -
TY - JOUR
AB - The therapeutic applications of silver nanoparticles (AgNPs) against
biomedical device-associated infections (BAI), by local delivery, are encountered
with risks of detachment, instability and nanotoxicity in physiological milieus. To
firmly anchor AgNPs onto modified biomaterial surfaces through tight
physicochemical interactions would potentially relieve these concerns. Herein, we
present a strategy for hierarchical TiO2/Ag coating, in an attempt to endow medical
titanium (Ti) with anticorrosion and antibacterial properties whilst maintaining
normal biological functions. In brief, by harnessing the adhesion and reactivity of
bioinspired polydopamine, silver nanoparticles were easily immobilized onto
peripheral surface and incorporated into interior cavity of a micro/nanoporous TiO2
ceramic coating in situ grown from template Ti. The resulting coating protected the
substrate well from corrosion and gave a sustained release of Ag+ up to 28 d. An
interesting germicidal effect, termed "trap-killing", was observed against
Staphylococcus aureus strain. The multiple osteoblast responses, i.e. adherence,
spreading, proliferation, and differentiation, were retained normal or promoted,
via a putative surface-initiated self-regulation mechanism. After subcutaneous
implantation for a month, the coated specimens elicited minimal, comparable
inflammatory responses relative to the control. Moreover, this simple and safe
functionalization strategy manifested a good degree of flexibility towards three-
dimensional sophisticated objects. Expectedly, it can become a prospective bench to
bedside solution to current challenges facing orthopedics. (C) 2015 Elsevier Ltd.
AN - WOS:000365373200019
AU - Jia, Z. J.
AU - Xiu, P.
AU - Li, M.
AU - Xu, X. C.
AU - Shi, Y. Y.
AU - Cheng, Y.
AU - Wei, S. C.
AU - Zheng, Y. F.
AU - Xi, T. F.
AU - Cai, H.
AU - Liu, Z. J.
DA - JAN
PY - 2016
SN - 0142-9612
1878-5905
SP - 203-222
ST - Bioinspired anchoring AgNPs onto micro-nanoporous TiO2 orthopedic coatings:
Trap-killing of bacteria, surface-regulated osteoblast functions and host responses
T2 - BIOMATERIALS
TI - Bioinspired anchoring AgNPs onto micro-nanoporous TiO2 orthopedic coatings:
Trap-killing of bacteria, surface-regulated osteoblast functions and host responses
VL - 75
ID - 6245
ER -
TY - JOUR
AB - Disease-associated nucleic acids, such as DNAs and miRNAs, are important
biomarkers for the diagnosis, prognosis and treatment guidance of human diseases.
Therefore, the accurate and sensitive detection of nucleic acid is of great
significance for the early diagnosis of diseases. DNA-scaffolded silver nanocluster
(DNA-Ag NC) is a new type of probe with good photostability and low toxicity that
has been widely used in biomedical analysis. In this work, a new universal sensing
platform based on target triggered labeling luminescent DNA-Ag NC for disease-
related nucleic acids detection was constructed. The assembled split DNA fragment
pair (C4AC4T and C3GT4) could be used as a template to develop a bright green
fluorescent Ag NC. According to this phenomenon, we devised two probe sequences DNA
1 and DNA 2, which could hybridize to the same one target and contained a different
split fragment of Ag NC’ scaffold. The target compelled the split fragments close
to each other through base pairing with DNA 1 and DNA 2, thus quantification of the
target could be achieved through measuring green fluorescence of Ag NC that
produced by assembled scaffold in ternary hybrid products. We applied this platform
successfully for miR-362, a potential biomarker of inflammatory bowel diseases
(IBD), or HIV-related DNA (hDNA) detection, achieving the detection limits of 6.5
nM and 1.7 nM, respectively. Both of the assays showed excellent reproducibility,
selectivity and potential applications in human serum samples. In summary, an
economic and convenient universal platform was developed for disease-associated
nucleic acid detection. © 2021 Elsevier B.V.
AU - Jia, Z.
AU - Tu, K.
AU - Xu, Q.
AU - Gao, W.
AU - Liu, C.
AU - Fang, B.
AU - Zhang, M.
C7 - 338734
DB - Scopus
DO - 10.1016/j.aca.2021.338734
KW - Biosensor
DNA-sliver nanocluster
Fluorescence probe
Nucleic acid
DNA
Humans
Limit of Detection
Metal Nanoparticles
Nucleic Acids
Silver
Biomarkers
Biomolecules
Diagnosis
Diseases
DNA sequences
Fluorescence
Nanoclusters
Probes
Scaffolds
biological marker
DNA fragment
fluorescent dye
gold nanoparticle
graphene oxide
green fluorescent protein
microRNA
microRNA 141
microRNA 21
microRNA 223
microRNA 362
nanomaterial
nucleic acid
oligonucleotide
unclassified drug
metal nanoparticle
silver
Ag$++$
Diagnoses of disease
Early diagnosis
Fluorescence probes
Human disease
Nucleic acid detection
Sensing platforms
Sensitive detection
Article
base pairing
colorimetry
DNA hybridization
high resolution transmission electron microscopy
human
human tissue
limit of detection
native polyacrylamide gel electrophoresis
nucleic acid analysis
particle size
prognosis
reproducibility
spectrofluorometry
zeta potential
genetic procedures
genetics
Biosensors
M3 - Article
PY - 2021
ST - A novel disease-associated nucleic acid sensing platform based on split DNA-
scaffolded sliver nanocluster
T2 - Analytica Chimica Acta
TI - A novel disease-associated nucleic acid sensing platform based on split DNA-
scaffolded sliver nanocluster
85107979349&doi=10.1016%2fj.aca.2021.338734&partnerID=40&md5=92b2eabf2284fe2e543f65
aabd5579c2
VL - 1175
ID - 5231
ER -
TY - JOUR
AB - Diabetic foot ulcers with complex healing wounds accompanied by bacterial
infection are considered a significant clinical problem which are made worse by the
lack of effective treatments. Traditional antibiotics and dressings have failed to
address wound infection and healing, and multifunctional combination therapies are
attractive for treating chronic wounds. In this study, arginine (Arg) was loaded
onto the surface of silver nanoclusters and encapsulated in a hydrogel to achieve
antibacterial, anti-inflammatory, angiogenic, and collagen deposition functions
through the slow release of Arg combined with silver nanoclusters. In vitro studies
indicated that Arg-Ag@H composites inhibited methicillin-resistant Staphylococcus
aureus and Escherichia coli by 94 and 97%, respectively. The inhibition of
bacterial biofilms reached 85%, and the migration ability of human venous
endothelial cells (HUVECs) increased by 50%. In vitro studies showed that Arg-Ag@H
composites increased the healing area of wounds by 26% and resulted in a 98% skin
wound-healing rate. Safety studies confirmed the excellent biocompatibility of Arg-
Ag@H. The results suggest that Arg-Ag@H offers new possibilities for treating
chronic diabetic wounds. © 2023 The Authors. Published by American Chemical
Society.
AU - Jiang, H.
AU - Xu, Q.
AU - Wang, X.
AU - Shi, L.
AU - Yang, X.
AU - Sun, J.
AU - Mei, X.
DB - Scopus
DO - 10.1021/acsomega.2c07266
IS - 14
M3 - Article
PY - 2023
SP - 12653-12663
ST - Preparation of Antibacterial, Arginine-Modified Ag Nanoclusters in the
Hydrogel Used for Promoting Diabetic, Infected Wound Healing
T2 - ACS Omega
TI - Preparation of Antibacterial, Arginine-Modified Ag Nanoclusters in the
Hydrogel Used for Promoting Diabetic, Infected Wound Healing
85151563995&doi=10.1021%2facsomega.2c07266&partnerID=40&md5=707fb86be328c8409061b1c
0f0b8a3bf
VL - 8
ID - 5035
ER -
TY - JOUR
AB - Konjac glucomannan is a biocompatible polysaccharide with high medicinal
potential. In this study, we prepared a hydrogel using an optimized crosslinking
konjac glucomannan and chitosan. Silver nanoparticles (AgNPs) were incorporated
into the hydrogel to enhance its antimicrobial property. This nanocomposite
hydrogel could absorb wound exudates due to its swelling ability, and showed self-
healing property that enabled structure stability. Moreover, as a carrier, the
hydrogel could modulate the release of silver ions burst, thereby reducing AgNPs
cytotoxicity. Rats models with infected skin defects were used to assess wound
healing. The results indicated that AgNPs hydrogels dressing could promote wound
healing and reduce inflammatory response, exhibiting great clinical application
potentials. © 2020
AU - Jiang, Y.
AU - Huang, J.
AU - Wu, X.
AU - Ren, Y.
AU - Li, Z.
AU - Ren, J.
DB - Scopus
DO - 10.1016/j.ijbiomac.2020.01.221
KW - Antibacterial hydrogel
Konjac glucomannan
Animals
Bandages
Cell Line
Chitosan
Delayed-Action Preparations
Hydrogels
Male
Mannans
Metal Nanoparticles
Mice
Rats
Silver
Wound Infection
chitosan
hydrogel
konjac glucomannan
mannan
silver
silver nanoparticle
unclassified drug
(1-6)-alpha-glucomannan
metal nanoparticle
adult
animal experiment
animal model
animal tissue
Article
controlled study
cross linking
cytotoxicity
Escherichia coli
flow kinetics
gelation
in vitro study
male
morphology
nonhuman
rat
skin defect
synthesis
wound healing
wound infection
animal
bandage
cell line
chemistry
delayed release formulation
microbiology
mouse
pathology
pharmacokinetics
pharmacology
Sprague Dawley rat
M3 - Article
PY - 2020
SP - 148-157
ST - Controlled release of silver ions from AgNPs using a hydrogel based on konjac
glucomannan and chitosan for infected wounds
TI - Controlled release of silver ions from AgNPs using a hydrogel based on konjac
glucomannan and chitosan for infected wounds
85078666101&doi=10.1016%2fj.ijbiomac.2020.01.221&partnerID=40&md5=a79e1691b84f8d4a3
002e3bb3683dafb
VL - 149
ID - 5295
ER -
TY - JOUR
AB - In the present study, bacterial cellulose (BC) based nanocomposite dressing
material was developed for third burn wound management by polydopamine (PD) coated
BC with in situ reduction of silver nanoparticles (BC-PDAg). BC-PDAg nanocomposite
was characterized to understand the morphological, physical and chemical
properties. Antimicrobial activity of BC-PDAg against burn wound specific pathogens
were significant. The in vitro cytotoxicity and proliferation studies revealed that
BC-PDAg nanocomposite is biocompatible and it supports cell proliferation. Further,
in vivo experiments on female albino Wistar rats confirmed that BC-PDAg was
effective in wound healing by promoting re-epithelization, and collagen deposition
as evidenced by histopathological analysis. Moreover, molecular gene expression
study has revealed that BC-PDAg promotes healing process by regulating the
expression of inflammatory, angiogenesis and growth factor genes. The overall
performance of BC-PDAg nanocomposite suggests that it could be used as promising
skin regenerative tool in modern medicine. © 2020 Elsevier Ltd
AU - Jiji, S.
AU - Udhayakumar, S.
AU - Maharajan, K.
AU - Rose, C.
AU - Muralidharan, C.
AU - Kadirvelu, K.
C7 - 116573
DB - Scopus
DO - 10.1016/j.carbpol.2020.116573
KW - Bacterial cellulose
Burn wound
Dressing material
Gene expression
Polydopamine
Cellulose
Deposition
Experimentation
Genes
Medicine
Processes
Reduction
Silver
Animals
Bacteria
Burns
Cell Proliferation
Cell Survival
Female
Gluconacetobacter xylinus
Metal Nanoparticles
Mice
Nanocomposites
NIH 3T3 Cells
Oxidation-Reduction
Rats
Rats, Wistar
Wound Healing
Biocompatibility
Cell proliferation
Metal nanoparticles
Tissue regeneration
antiinfective agent
cellulose
metal nanoparticle
nanocomposite
silver
Collagen deposition
Gene expression studies
Histopathological analysis
In-vivo experiments
Physical and chemical properties
Wound management
animal
bacterium
burn
cell proliferation
cell survival
chemistry
drug effect
female
mouse
NIH 3T3 cell line
procedures
rat
Wistar rat
wound healing
M3 - Article
PY - 2020
ST - Bacterial cellulose matrix with in situ impregnation of silver nanoparticles
via catecholic redox chemistry for third degree burn wound healing
T2 - Carbohydrate Polymers
TI - Bacterial cellulose matrix with in situ impregnation of silver nanoparticles
via catecholic redox chemistry for third degree burn wound healing
85086434640&doi=10.1016%2fj.carbpol.2020.116573&partnerID=40&md5=6df8e5901f582f148e
5cc81e87612185
VL - 245
ID - 5342
ER -
TY - JOUR
AB - Objective： To investigate the intervention effect of Danggui Buxuetang on
oxidative stress and inflammatory response in diabetic kidney disease （DKD） rats
from its improvement of podocyte mitochondrial dysfunction. Method：SD rats were
randomly divided into the control group and modeling group， and the ones in the
latter group rats were fed a high-glucose and high-fat diet and then
intraperitoneally injected with a small dose of streptozotocin（STZ）for inducing
type 2 diabetes. The successfully modeled rats were randomized into the model
group，high-and low-dose（1.44 and 0.72 g·kg-1）Danggui Buxuetang groups，and
irbesartan（0.017 g·kg-1）group and gavaged with the corresponding drugs，while
those in the normal and model groups with an equal volume of normal saline. After
20 weeks of drug intervention，the urinary microalbumin-tourine creatinine ratio
（UACR） and serum malondialdehyde （MDA） content and manganese superoxide
dismutase（MnSOD）activity in each group were measured. The pathological changes in
renal tissue were observed by Masson trichrome staining，and periodic acid-silver
metheramine（PASM）staining，followed by the observation of ultrastructural changes
in podocytes under the transmission electron microscope（TEM）. The expression
level of reactive oxygen species（ROS）in rat kidney tissue was detected using a
fluorescent probe dihydroethidium （DHE）. The protein expression levels of
peroxisome proliferator-activated receptor γ coactivator-1α（PGC-1α），nucleotide-
binding domain like receptor protein 3（NLRP3），and Wilms tumor protein-1（WT-
1）were measured by immunohistochemistry（IHC），and the expression levels of
NLRP3， interleukin-1β（IL-1β），and WT-1 in podocytes by
immunofluorescence（IF）assay. The mRNA expression levels of PGC-1α and NLRP3 in
the renal tissues were determined by real-time fluorescence quantitative polymerase
chain reaction（Real-time PCR），and the protein expression levels of PGC-
1α，MnSOD，NLRP3，and IL-1β were assayed by Western blot. Result： Compared with the
normal group，the model group exhibited elevated UACR and MDA content，weakened
MnSOD activity（P<0.01），glomerular hypertrophy，thickened basement membrane，
mesangial hyperplasia， increased extracellular matrix， K-W nodules， podocyte
mitochondrial swelling， disordered mitochondrial cristae， foot process fusion or
loss， vacuolization， increased ROS （P<0.01），enhanced NLRP3 and IL-1β but
diminished WT-1 expression in podocytes，down-regulated PGC-1α mRNA
expression（P<0.01）and PGC-1α and MnSOD protein expression（P<0.01），and up-
regulated NLRP3 mRNA expression and NLRP3 and IL-1β protein expression（P<0.01）.
Compared with the model group， Danggui Buxuetang high-dose group significantly
decreased UACR and MDA，enhanced MnSOD activity （P<0.05，P<0.01），improved renal
histopathology and podocyte mitochondrial ultrastructure，decreased ROS
（P<0.05，P<0.01）and NLRP3 and IL-1β expression in podocytes，enhanced WT-1
expression in podocytes，upregulated the mRNA and protein levels of PGC-1α and
MnSOD，and down-regulated the mRNA and protein levels of NLRP3 and IL-
1β（P<0.05，P<0.01）. Conclusion： Danggui Buxuetang alleviates oxidative stress，
·32· reduces inflammatory response，protects kidney，and delays the progression of
DKD possibly by improving the mitochondrial dysfunction in podocytes of DKD rats. ©
2022, China Academy of Chinese Medical Sciences Institute of Chinese Materia
Medica. All rights reserved.
AU - Jin, H. C.
AU - Qiang, J. W.
AU - Zhang, G. W.
AU - Liang, S. R.
AU - Guo, D. Z.
DB - Scopus
DO - 10.13422/j.cnki.syfjx.20212442
IS - 3
KW - Danggui Buxuetang
diabetic kidney disease （DKD）
inflammatory response
mitochondrial dysfunction
podocytes；oxidative stress
adegramotide
Chinese drug
cryopyrin
Danggui Buxue Tang
interleukin 1beta
irbesartan
malonaldehyde
peroxisome proliferator activated receptor gamma coactivator 1alpha
streptozocin
unclassified drug
albumin to creatinine ratio
animal experiment
animal model
animal tissue
apoptosis
Article
controlled study
drug megadose
enzyme activity
fluorescence quantitative polymerase chain reaction
gene expression
histopathology
inflammation
lipid diet
low drug dose
nonhuman
oxidative stress
podocyte
protein expression
rat
Western blotting
M3 - Article
PY - 2022
SP - 31-40
ST - Danggui Buxuetang Alleviates Oxidative Stress and Inflammation in Diabetic
Kidney Disease Rats by Improving Mitochondrial Dysfunction of Podocytes
T2 - Chinese Journal of Experimental Traditional Medical Formulae
TI - Danggui Buxuetang Alleviates Oxidative Stress and Inflammation in Diabetic
Kidney Disease Rats by Improving Mitochondrial Dysfunction of Podocytes
85129696156&doi=10.13422%2fj.cnki.syfjx.20212442&partnerID=40&md5=ea68017d59bbbca24
d269d3d87dc7509
VL - 28
ID - 5074
ER -
TY - JOUR
AB - Mast cells play critical roles in allergic disorders such as atopic
dermatitis and allergic asthma. The aim of this study was to investigate the anti-
inflammatory and anti-asthmatic activities of 1,6-O,O-diacetylbritannilactone
(OODBL) isolated from Inula japonica Thunb. (I. japonica) in a murine asthma model
and bone marrow-derived mast cells (BMMCs). In an ovalbumin-induced asthma model,
OODBL administration attenuated the airway hyper-responsiveness induced by
aerosolized methacholine and serum IgE level in asthmatic mice. In vitro system, we
found that OODBL reduced leukotriene C4 production and degranulation through the
suppression of cytosolic phospholipase A2 phosphorylation and phospholipase Cγ-
mediated Ca2+ influx in IgE/antigen-stimulated BMMCs. Taken together, OODBL may
have therapeutic potential in the treatment of allergic diseases such as asthma. ©
2017 Informa UK Limited, trading as Taylor & Francis Group.
AU - Jin, M.
AU - Kim, S.
AU - Qin, N.
AU - Chen, X.
AU - Ji, N.
AU - Tang, S. A.
AU - Kong, D.
AU - Lee, E.
AU - Duan, H.
DB - Scopus
DO - 10.1080/08923973.2017.1318911
IS - 4
KW - asthma
inflammation
leukotriene C4
OODBL
Animals
Anti-Allergic Agents
Anti-Asthmatic Agents
Asthma
Bone Marrow Cells
Cell Degranulation
Dermatitis, Atopic
Female
Immunoglobulin E
Lactones
Leukotriene C4
Mast Cells
Mice
Mice, Inbred BALB C
Ovalbumin
Phosphorylation
Respiratory Hypersensitivity
Sesquiterpenes
Signal Transduction
1,6 o,o diacetylbritannilactone
antiasthmatic agent
calcium
dexamethasone
immunoglobulin E
membrane phospholipid
methacholine
montelukast
ovalbumin
phospholipase A2
phospholipase C gamma
silver
unclassified drug
antiallergic agent
lactone
O, O-diacetylbritannilactone
sesquiterpene
animal cell
animal experiment
animal model
anti asthmatic activity
Article
basophil degranulation
bone marrow derived mast cell
calcium cell level
calcium transport
cell activation
cell viability
controlled study
cytotoxicity
drug activity
female
IC50
immunoglobulin blood level
in vitro study
Inula
Inula japonica
male
mast cell
mouse
MTS assay
nonhuman
priority journal
animal
atopic dermatitis
Bagg albino mouse
bone marrow cell
chemically induced
degranulation
disease model
drug effects
metabolism
phosphorylation
respiratory tract allergy
signal transduction
M3 - Article
PY - 2017
SP - 173-179
ST - 1,6-O,O-Diacetylbritannilactone suppresses activation of mast cell and airway
hyper-responsiveness
T2 - Immunopharmacology and Immunotoxicology
TI - 1,6-O,O-Diacetylbritannilactone suppresses activation of mast cell and airway
hyper-responsiveness
85018186234&doi=10.1080%2f08923973.2017.1318911&partnerID=40&md5=9dd31abfd570be669b
8c57a4f170a97b
VL - 39
ID - 5533
ER -
TY - JOUR
AB - Recently revised OECD inhalation toxicity testing guidelines require
measurements of lung burden immediately after and for periods following exposure
for nanomaterials. Lung burden is a function of pulmonary deposition and retention
of nanoparticles. Using lung burden studies as per OECD guidelines, it may be
possible to assess clearance mechanisms of nanoparticles. In this study, male rats
were exposed to silver nanoparticle (AgNP) aerosols (18.1–19.6 nm) generated from a
spark generator. Exposure groups consisted of (1) control (fresh air), (2) low
(31.2 ± 8.5 µg/m3), (3) moderate (81.8 ± 11.4 µg/m3), and (4) high concentrations
(115.6 ± 30.5 µg/m3). Rats were exposed for 6-h/day, 5-days/week for 4 weeks (28-
days) based on the revised OECD test guideline 412. Bronchoalveolar lavage (BAL)
fluids were collected on post-exposure observation (PEO)-1 and PEO-7 days and
analyzed for inflammatory cells and inflammatory biomarkers. The lung burdens of Ag
from AgNPs were measured on PEO-1, PEO-7, and PEO-28 days to obtain quantitative
mass concentrations per lung. Differential counting of blood cells and inflammatory
biomarkers in BAL fluid and histopathological evaluation of lung tissue indicated
that exposure to the high concentrations of AgNP aerosol induced inflammation at
PEO-1, slowly resolved at PEO-7 and completely resolved at PEO-28 days. Lung burden
measurement suggested that Ag from AgNPs was cleared through two different modes;
fast and slow clearance. The fast clearance component was concentration-dependent
with half-times ranging from two to four days and clearance rates of
0.35–0.17/day−1 from low to high concentrations. The slow clearance had half-times
of 100, 57, and 76 days and clearance rates of 0.009, 0.012, and 0.007/day−1 for
the high, moderate and low concentration exposure. The exact mechanism of clearance
is not known currently. The fast clearance component which was concentration-
dependent could be dependent on the dissolution of AgNPs and the slow clearance
would be due to slow clearance of the low dissolution AgNPs secondary particles
originating from silver ions reacting with biogenic anions. These secondary AgNPs
might be cleared by mechanisms other than dissolution such as mucociliary
escalation, translocation to the lymphatic system or other organs. © 2020,
Springer-Verlag GmbH Germany, part of Springer Nature.
AU - Jo, M. S.
AU - Kim, J. K.
AU - Kim, Y.
AU - Kim, H. P.
AU - Kim, H. S.
AU - Ahn, K.
AU - Lee, J. H.
AU - Faustman, E. M.
AU - Gulumian, M.
AU - Kelman, B.
AU - Yu, I. J.
DB - Scopus
DO - 10.1007/s00204-020-02660-2
IS - 3
KW - Lung burden
Lung clearance
Subacute inhalation
Aerosols
Animals
Inhalation Exposure
Male
Metabolic Clearance Rate
Metal Nanoparticles
Particle Size
Rats
Silver
albumin
biological marker
silver
silver nanoparticle
metal nanoparticle
aerosol
animal cell
animal experiment
animal model
animal tissue
Article
blood cell count
body weight
clearance
controlled study
dissolution
exposure
fibrosing alveolitis
food intake
histopathology
inflammation
inflammatory cell
lung burden
lung clearance
lung lavage
lymphocyte percentage
male
mean corpuscular hemoglobin
mean platelet volume
monocyte
monocyte percentage
nonhuman
organ weight
Organisation for Economic Co-operation and Development
practice guideline
priority journal
prothrombin time
rat
thromboplastin time
toxicokinetics
animal
metabolic clearance rate
metabolism
particle size
M3 - Article
PY - 2020
SP - 773-784
ST - Mode of silver clearance following 28-day inhalation exposure to silver
nanoparticles determined from lung burden assessment including post-exposure
observation periods
TI - Mode of silver clearance following 28-day inhalation exposure to silver
nanoparticles determined from lung burden assessment including post-exposure
observation periods
85081753170&doi=10.1007%2fs00204-020-02660-
2&partnerID=40&md5=0b26594653055002133a8e68a0a10968
VL - 94
ID - 5321
ER -
TY - JOUR
AB - This review is concerned with evaluating the toxicity associated with human
exposure to silver and gold nanoparticles (NPs), due to the relative abundance of
toxicity data available for these particles, when compared to other metal
particulates. This has allowed knowledge on the current understanding of the field
to be gained, and has demonstrated where gaps in knowledge are. It is anticipated
that evaluating the hazards associated with silver and gold particles will
ultimately enable risk assessments to be completed, by combining this information
with knowledge on the level of human exposure. The quantity of available hazard
information for metals is greatest for silver particulates, due to its widespread
inclusion within a number of diverse products (including clothes and wound
dressings), which primarily arises from its antibacterial behaviour. Gold has been
used on numerous occasions to assess the biodistribution and cellular uptake of NPs
following exposure. Inflammatory, oxidative, genotoxic, and cytotoxic consequences
are associated with silver particulate exposure, and are inherently linked. The
primary site of gold and silver particulate accumulation has been consistently
demonstrated to be the liver, and it is therefore relevant that a number of in
vitro investigations have focused on this potential target organ. However, in
general there is a lack of in vivo and in vitro toxicity information that allows
correlations between the findings to be made. Instead a focus on the tissue
distribution of particles following exposure is evident within the available
literature, which can be useful in directing appropriate in vitro experimentation
by revealing potential target sites of toxicity. The experimental design has the
potential to impact on the toxicological observations, and in particular the use of
excessively high particle concentrations has been observed. As witnessed for other
particle types, gold and silver particle sizes are influential in dictating the
observed toxicity, with smaller particles exhibiting a greater response than their
larger counterparts, and this is likely to be driven by differences in particle
surface area, when administered at an equal-mass dose. A major obstacle, at
present, is deciphering whether the responses related to silver nanoparticulate
exposure derive from their small size, or particle dissolution contributes to the
observed toxicity. Alternatively, a combination of both may be responsible, as the
release of ions would be expected to be greater for smaller particles. © 2010
Informa UK Ltd.
AU - Hutchison, G.
AU - Christensen, F. M.
AU - Peters, S.
AU - Hankin, S.
AU - Stone, V.
DB - Scopus
DO - 10.3109/10408440903453074
IS - 4
KW - Gold
Metals
Nanoparticle
Nanotoxicology
Silver
Dust
Humans
Nanoparticles
Particle Size
Particulate Matter
Risk Assessment
Silver Compounds
Tissue Distribution
amoxicillin
gold nanoparticle
metronidazole
nanoparticle
silver nanoparticle
sulfadiazine silver
argyria
arthritis
burn
clinical trial
controlled study
cytotoxicity
diabetes mellitus
drug absorption
drug distribution
drug effect
drug transport
genotoxicity
human
inflammation
intestine ulcer
liver toxicity
lung toxicity
nanotoxicology
neoplasm
nonhuman
oxidative stress
particle size
review
risk assessment
skin discoloration
tissue distribution
toxicity
toxicity testing
wound dressing
M3 - Review
PY - 2010
SP - 328-346
ST - A review of the in vivo and in vitro toxicity of silver and gold
particulates: Particle attributes and biological mechanisms responsible for the
observed toxicity
T2 - Critical Reviews in Toxicology
TI - A review of the in vivo and in vitro toxicity of silver and gold
particulates: Particle attributes and biological mechanisms responsible for the
observed toxicity
77949780454&doi=10.3109%2f10408440903453074&partnerID=40&md5=93f061be04dc41114d6316
5eabf8a5ed
VL - 40
ID - 5688
ER -
TY - JOUR
AB - The effects of nanomaterials (NMs) on biological systems, especially their
ability to stimulate inflammatory responses requires urgent investigation. We
evaluated the response of the human differentiated HL60 neutrophil-like cell line
to NMs. It was hypothesised that NM physico-chemical characteristics would
influence cell responsiveness by altering intracellular Ca2+ concentration [Ca2+]i
and reactive oxygen species production.Cells were exposed (1.95-125μg/ml, 24h) to
silver (Ag), zinc oxide (ZnO), titanium dioxide (TiO2), multi-walled carbon
nanotubes (MWCNTs) or ultrafine carbon black (ufCB) and cytotoxicity assessed
(alamar blue assay). Relatively low (TiO2, MWCNTs, ufCB) or high (Ag, ZnO)
cytotoxicity NMs were identified. Sub-lethal impacts of NMs on cell function were
investigated for selected NMs only, namely TiO2, Ag and ufCB. Only Ag stimulated
cell activation. Within minutes, Ag stimulated an increase in [Ca2+]i (in Fura-2
loaded cells), and a prominent inward ion current (assessed by electrophysiology).
Within 2-4h, Ag increased superoxide anion release and stimulated cytokine
production (MCP-1, IL-8) that was diminished by Ca2+ inhibitors or trolox. Light
microscopy demonstrated that cells had an activated phenotype.In conclusion NM
toxicity was ranked; Ag>ufCB>TiO2, and the battery of tests used provided insight
into the mechanism of action of NM toxicity to guide future testing strategies. ©
2015 Elsevier Ltd.
AU - Johnston, H.
AU - Brown, D. M.
AU - Kanase, N.
AU - Euston, M.
AU - Gaiser, B. K.
AU - Robb, C. T.
AU - Dyrynda, E.
AU - Rossi, A. G.
AU - Brown, E. R.
AU - Stone, V.
DB - Scopus
DO - 10.1016/j.tiv.2015.04.021
IS - 5
KW - Ca2+
Mechanism
Nanomaterial
Neutrophil
Toxicity
Calcium
Cell Survival
Cytokines
HL-60 Cells
Humans
Nanostructures
Nanotubes, Carbon
Silver
Soot
Superoxides
Titanium
Zinc Oxide
calcium
calcium channel blocking agent
carbon nanoparticle
fura 2
interleukin 8
nanomaterial
silver nanoparticle
superoxide
trolox C
carbon nanotube
cytokine
silver
soot
titanium
titanium dioxide
zinc oxide
Article
calcium cell level
cell function
controlled study
cytokine production
cytotoxicity
electrophysiology
HL 60 cell line
human
human cell
ion current
microscopy
phenotype
physical chemistry
resazurin assay
cell survival
drug effects
metabolism
toxicity
M3 - Article
PY - 2015
SP - 1172-1184
ST - Mechanism of neutrophil activation and toxicity elicited by engineered
nanomaterials
TI - Mechanism of neutrophil activation and toxicity elicited by engineered
nanomaterials
84930001176&doi=10.1016%2fj.tiv.2015.04.021&partnerID=40&md5=744eea70177cc486cba4a4
4d7d3e9984
VL - 29
ID - 5632
ER -
TY - JOUR
AB - Ultra-fine grained biodegradable Mg-based Mg1Zn1Mn0.3 Zr - HA and
Mg4Y5.5Dy0.5 Zr - 45S5 Bioglass composites have shown great medical potential. Two
types of these Mg-based biomaterials subjected to different treatments were tested
and as shown earlier they are biocompatible. The aim of the study is to determine
how much culture media incubated with these ultra-fine trained Mg-based composites
can cause inflammatory reactions and /or periodontal cell death. The incubation of
composites in the medium releases metal ions into the solution. It can be assumed
that this process is permanent and also occurs in the human body. The results have
shown that the effect of proinflammatory IL-6 and TNF-[Formula presented] cytokines
results in the strongest production of the acute phase proteins in the first day on
the Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. % Ag HF-treated biocomposite after immersion for
2 h in 40 % HF and then the fastest decrease in these processes on the third day.
In turn, the inflammatory process induced on the Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. %
Ag biomaterial, in BAX / BCL ratio assessment, is the strongest on the third day
and maintains a significantly high level on the following day, which, at the same
time, confirms its persistence and development. In addition, these results confirm
the successively generated necrotic processes. Ions can induce inflammatory
reactions, which in the case of the implant may take a long time, which results in
the loss of the implant. Even if the material is biocompatible in rapid in-vitro
tests, it can induce inflammation in the body after some time due to the release of
ions. Not every treatment improves the material's properties in terms of subsequent
safety. [Formula presented] © 2019
AU - Jurczyk, M. U.
AU - Żurawski, J.
AU - Wirstlein, P. K.
AU - Kowalski, K.
AU - Jurczyk, M.
C7 - 102796
DB - Scopus
DO - 10.1016/j.micron.2019.102796
KW - Biomaterials
Cytokines: IL-6 and TNF-α
Inflammatory cells
Mg
Cells, Cultured
Ceramics
Glass
Humans
Inflammation
Interleukin-6
Magnesium
Magnesium Compounds
Materials Testing
Osteoblasts
Periodontium
Surface Properties
Biocompatibility
Cell death
Dysprosium alloys
Manganese alloys
Metal ions
Proteins
Silver
Zinc alloys
Zirconium
bioactive glass 45S5
biomaterial
glass
IL6 protein, human
interleukin 6
magnesium
magnesium derivative
Acute phase proteins
Cytokines
Different treatments
Inflammatory process
Mg based composites
Ultra-fine-grained
biosynthesis
cell culture
ceramics
cytology
drug effect
human
inflammation
materials testing
osteoblast
periodontium
pharmacology
procedures
surface property
Magnesium alloys
M3 - Article
PY - 2020
ST - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
T2 - Micron
TI - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
85076014504&doi=10.1016%2fj.micron.2019.102796&partnerID=40&md5=cc127a234db0df5ceaa
609f319702eda
VL - 129
ID - 5348
ER -
TY - JOUR
AB - Ultra-fine grained biodegradable Mg-based Mg1Zn1Mn0.3 Zr - HA and
Mg4Y5.5Dy0.5 Zr - 45S5 Bioglass composites have shown great medical potential. Two
types of these Mg-based biomaterials subjected to different treatments were tested
and as shown earlier they are biocompatible. The aim of the study is to determine
how much culture media incubated with these ultra-fine trained Mgbased composites
can cause inflammatory reactions and /or periodontal cell death. The incubation of
composites in the medium releases metal ions into the solution. It can be assumed
that this process is permanent and also occurs in the human body. The results have
shown that the effect of proinflammatory IL-6 and TNF-e cytokines results in the
strongest production of the acute phase proteins in the first day on the
Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. % Ag HF-treated biocomposite after immersion for 2 h
in 40 % HF and then the fastest decrease in these processes on the third day. In
turn, the inflammatory process induced on the Mg1Zn1Mn0.3 Zr-5 wt.% HA-1 wt. % Ag
biomaterial, in BAX / BCL ratio assessment, is the strongest on the third day and
maintains a significantly high level on the following day, which, at the same time,
confirms its persistence and development. In addition, these results confirm the
successively generated necrotic processes. Ions can induce inflammatory reactions,
which in the case of the implant may take a long time, which results in the loss of
the implant. Even if the material is biocompatible in rapid in-vitro tests, it can
induce inflammation in the body after some time due to the release of ions. Not
every treatment improves the material's properties in terms of subsequent safety.
AN - WOS:000508751000010
AU - Jurczyk, M. U.
AU - Zurawski, J.
AU - Wirstlein, P. K.
AU - Kowalski, K.
AU - Jurczyk, M.
DA - FEB
DO - 10.1016/j.micron.2019.102796
PY - 2020
SN - 0968-4328
1878-4291
ST - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
T2 - MICRON
TI - Response of inflammatory cells to biodegradable ultra-fine grained Mg-based
composites
VL - 129
ID - 6375
ER -
TY - JOUR
AB - Factors involved in wound healing and their interdependence are not yet fully
understood; nevertheless, new prospects for therapy to favor speedy and optimal
healing are emerging. Reports about wound healing modulation by local application
of simple and natural agents abound even in the recent literature, however, most
are anecdotal and lack solid scientific evidence. We describe the effect of silver
sulfadiazine and moist exposed burn ointment (MEBO), a recently described burn
ointment of herbal origin, on mast cells and several wound healing cytokines (bFGF,
IL-1, TGF-beta, and NGF) in the rabbit experimental burn model. The results
demonstrate that various inflammatory cells, growth factors and cytokines present
in the wound bed may be modulated by application of local agents with drastic
effects on their expression dynamics with characteristic temporal and spatial
regulation and changes in the expression pattern. Such data are likely to be
important for the development of novel strategies for wound healing since they shed
some light on the potential formulations of temporally and combinatory optimized
therapeutic regimens. (C) 2006 Elsevier Ltd and ISBI. All rights reserved.
AN - WOS:000250361600012
AU - Jurjus, A.
AU - Atiyeh, B. S.
AU - Abdallah, I. M.
AU - Jurjus, R. A.
AU - Hayek, S. N.
AU - Abou Jaoude, M.
AU - Gerges, A.
AU - Tohme, R. A.
DA - NOV
DO - 10.1016/j.burns.2006.10.406
IS - 7
PY - 2007
SN - 0305-4179
1879-1409
SP - 892-907
ST - Pharmacological modulation of wound healing in experimental burns
T2 - BURNS
TI - Pharmacological modulation of wound healing in experimental burns
VL - 33
ID - 6780
ER -
TY - JOUR
AB - The objective of this review is to form a short compilation of phytochemical
screening, pharmaceutical and pharmacological profile of the plant Cochlospermum
religiosum. Although the plant is of importance and is widely used in traditional
system of medicine, a review article based on the phytochemical and pharmacological
screening of Cochlospermum religiosum is not upto date not reported. The various
histochemical studies revealed that this plant contains numerous primary and
secondary metabolites as its constituents such as flavanoids, steroids, tannins,
glycosides, alkaloids, phenols, starch grains and crystals. The gum Katira obtained
from the stem bark of Cochlospermum religiosum has wide variety of applications in
pharmaceutical industry as adjuvant in colon targeted drug delivery formulations
like Azathioprine, as a matrix coating polymers, suspending agent and sustain
release adjuvant for nimusulide and Etodalac formulations respectively. The silver
nanoparticles biosynthesized from this plant are found to possess antimicrobial
activity against various pathogenic microbes. Moreover, many research studies have
been conducted to prove the plant's potential as antimicrobial agents. The
bioactive secondary metabolite Myricetin which has wide array of biochemical
properties, such as antineoplastic, anti-carcinogenic, antioxidant activity and
anti-inflammatory effects has been identified and isolated from leaf and callus by
using different techniques such as IR spectra and HPTLC. This review focuses on the
phytochemistry, pharmaceutical and pharmacological actions of Cochlospermum
religiosum.
AU - Jyothi, Y.
AU - Sangeetha, D.
DB - Scopus
IS - 5
KW - Cochlospermum religiosum
Gum Katira
Myrcetin
Yello silk tree
alkaloid
Cochlospermum religiosum extract
flavanoid
glycoside
phenol
plant extract
silver nanoparticle
steroid
tannin
unclassified drug
Alstonia
Bacillus subtilis
bark
callus (plant)
cell viability
drug cytotoxicity
drug isolation
drug mechanism
drug screening
Enterobacter aerogenes
Escherichia coli
histochemistry
in vitro study
liver protection
Micrococcus
nonhuman
phytochemistry
plant leaf
plant stem
Proteus vulgaris
Review
Xanthomonas axonopodis
Xanthomonas oryzae
M3 - Review
PY - 2016
SP - 272-277
ST - Cochlospermum religiosum (Linn): A phytopharmacological review
T2 - Research Journal of Pharmaceutical, Biological and Chemical Sciences
TI - Cochlospermum religiosum (Linn): A phytopharmacological review
84987750969&partnerID=40&md5=25e3df5b805d54308b527d4b611fa7d5
VL - 7
ID - 5489
ER -
TY - JOUR
AB - Objective. To study the current Russian and foreign literature dedicated to
the problem of application of organometallic compounds immobilized on drug delivery
in the treatment of purulent-inflammatory disease of the skin and soft tissues.
Methods. The modern Russian and foreign literature, available in the Pubmed,
Medline, Springer, Scopus, e-LIBRARY databases were reviewed according to the
problems of purulent-inflammatory diseases, skin and soft tissue infections, the
integrated approach to the treatment of purulent-inflammatory diseases, synthesis,
immobilized organometallic compounds. Results. The observational study of the
specific recent achievements in the modification of antimicrobial biomaterials is
presented. Metal ions have a broad range of antimicrobial activity (especially on
proliferation and remodeling), possess by bacteriostatic and bactericidal effect,
demonstrate multiple inhibitory effects against bacterial strains and have been
proven effective in improving wound healing in all its phases. Natural products and
especially biologically active metals such as silver, copper, zinc and germanium,
are believed to be an alternative for the development of perspective biomaterials
with antimicrobial properties. In recent years, new approach for the production and
application of therapeutic and diagnostic drugs based on the immobilization or
grafting of drug substances on polymer carriers has been developed. At present,
namely the immobilized compounds that have opened the way to the creation of
prolonged-action drugs with low toxicity and allergenicity. Conclusion. Template
synthesis of new organometallic drug compounds is considered to be a promising
direction in the wound infection treatment, which requires further experimental and
clinical study. © 2021 Vitebsk State Medical University. All rights reserved.
AU - Kadomtsevа, A. V.
AU - Zarubenko, P. A.
AU - Loginova, L. B.
DB - Scopus
DO - 10.18484/2305-0047.2021.3.334
IS - 3
KW - Biometals
Immobilized compounds
Nanoparticles
Organometallic frameworks
Purulent-inflammatory processes
Synthesis
M3 - Article
PY - 2021
SP - 334-346
ST - РОЛЬ ИММОБИЛИЗОВАННЫХ МЕТАЛЛООРГАНИЧЕСКИХ СОЕДИНЕНИЙ В КОМПЛЕКСНОМ ЛЕЧЕНИИ
ГНОЙНО-ВОСПАЛИТЕЛЬНЫХ ПРОЦЕССОВ КОЖИ И МЯГКИХ ТКАНЕЙ
T2 - Novosti Khirurgii
TI - The role of immobilized metal-organic compounds in the complex treatment of
purulent-inflammatory disease of skin and soft tissues
85111759564&doi=10.18484%2f2305-
0047.2021.3.334&partnerID=40&md5=5fcfa443a09b6ff8471e9f5d4ab619fe
VL - 29
ID - 5257
ER -
TY - JOUR
AB - To study acute lung toxicity of various doses of colloidal silver
nanoparticles (Ag-NPs), mice were intratracheally instilled with 0, 10, 100, 1000
or 10,000 ppm of Ag-NPs. Histopathology, autometallography (AMG) and
immunohistochemistry were determined at 1, 3, 7 and 15 days post-
exposure.Instillation of 100, 1,000 and 10,000 ppm Ag-NPs produced moderate to
severe necrotizing bronchitis and alveolitiswith hypertrophy and hyperplasia of
alveolar epithelial cells. The severity of the pulmonary inflammation and damage
increased in a dose-dependent manner. Concomitant lamininimmunohistochemical
findings generally correlated with pulmonary lesions. Interleukin 1- beta (IL-1β)
and tumor necrotic factor-alpha (TNF-α) positive immunostaining were found in the
inflammatory lesions in lungs of treated animals. Superoxide dismutase (SOD) and
metallothionine (MT) expression occurred in particle laden AMs and lung epithelial
cells, which correlated with inflammatory sites and particle aggregated areas. AMG
gains were found inparticle laden AMs, alveolar epithelial cells and macrophages in
hilarlymph nodes. These findings suggest that instillation of AgNPs causes acute
lung inflammation and tissue damage in a concentration-dependent manner. IL-1β and
TNF-α may involve in the pathogenesis of the acute lung toxicity. Oxidative stress
may underlie the lung tissue injury. Moreover, the expression of MT in tissues
responded to AgNPs accumulation.
AU - Kaewamatawong, T.
AU - Banlunara, W.
AU - Maneewattanapinyo, P.
AU - Thammacharoen, C.
AU - Ekgasit, S.
DB - Scopus
IS - 3
KW - Acute
Colloidal silver nanoparticles
Lung toxicity
Mouse
M3 - Article
PY - 2013
SP - 383-390
ST - Acute pulmonary toxicity caused by single intratracheal instillation of
various doses of colloidal silver nanoparticles in mice: Pathological changes,
particle bioaccumulation and metallothionien protein expression
T2 - Thai Journal of Veterinary Medicine
TI - Acute pulmonary toxicity caused by single intratracheal instillation of
various doses of colloidal silver nanoparticles in mice: Pathological changes,
particle bioaccumulation and metallothionien protein expression
84897930059&partnerID=40&md5=d9e3b100c4c3f2ee6323e4bcae74fc3b
VL - 43
ID - 5723
ER -
TY - JOUR
AB - Morphological changes of liver and spleen under the impact of dextran-
polyacrylamide polymers and their effects as carriers of silver and gold
nanoparticles. Kaleinikova O.M., Kurovska V.O., Byelinska I.V., Kutsevol N.V.,
Blashkiv T. V. The possibility of usage of polymer nanocomposites is being
intensively studied today with a purpose of their application in medicine,
espessialy in oncology. At the experimental stage it is important to determine the
mechanisms of the influence of such compounds on the bod V and their own possible
undesirable effects. Aim - to study the effect of the treatment with maximal doses
of the dextran-polyacrylamide polymers and their effect as carriers of silver and
gold nanoparticles on the spleen and liver. Histological examination of
micropreparations of the spleen and liver by the standard method with hematoxylin-
eosin staining was made. As a result of the treatment with nonionic (D-g-PAA) and
anionic (D-g-PAA (PE» polymer matrices, changes which occurred in the spleen
indicate an increase in the production of all blood cells. These phenomena were
absent when silver and gold nanoparticles were included in the matrix. In the
liver, treatment with D-g-PAA and D-g-PAA (PE) caused a disorder of hepatic
circulation, focal infiltration by inflammatory cells and death of liepatocytes by
necrosis. The addition of nanoparticles triggered other mechanisms of alteration,
which manifested themselves in excessive accumulation of glycogen, fatty
infiltration ofhepatocytes, and cell death, mainly through apoptosis. However;
along with this, sigfis of an incomplete regenerative response of the liver were
revealed. Morphological changes caused by the treatment with maximal doses of the
tested substances indicate their toxic effect, especially on the liver. Further
researches are needed to establish the optimal doses and the frequency of their
administration, which can be used for therapeutic purposes, including the
interaction of studied polymers with blood cells. © 2023 Dnipro State Medical
University. All rights reserved.
AU - Kaleinikova, O. M.
AU - Kurovska, V. O.
AU - Byelinska, I. V.
AU - Kutsevol, N. V.
AU - Blashkiv, T. V.
DB - Scopus
DO - 10.26641/2307-0404.2023.1.275855
IS - 1
KW - dextran-polyacrylamide polymers
liver
silver and gold nanoparticles
spleen
toxicity
M3 - Article
PY - 2023
SP - 28-36
ST - MORPHOLOGICAL CHANGES OF SPLEEN AND LIVER UNDER THE IMPACT OF DEXTRAN-
POLYACRYLAMIDE POLYMERS AND THEIR EFFECTS AS CARRIERS OF SILVER AND GOLD
NANOPARTICLES
T2 - Medicni Perspektivi
TI - MORPHOLOGICAL CHANGES OF SPLEEN AND LIVER UNDER THE IMPACT OF DEXTRAN-
POLYACRYLAMIDE POLYMERS AND THEIR EFFECTS AS CARRIERS OF SILVER AND GOLD
NANOPARTICLES
85160039576&doi=10.26641%2f2307-
0404.2023.1.275855&partnerID=40&md5=fb2b442345c61836239a2ab7be222b3b
VL - 28
ID - 4972
ER -
TY - JOUR
AB - The analgesic, anti-pyretic and anti-inflammatory effects of FI-302, Ar-(3-
pip-eridinopropyl)-4-methyl-6-trifluoromethyl-furo[3,2-ft]indole-2-carboxamide, a
newly synthesized tricyclic compound, were investigated in comparison with those of
nonsteroidal anti-inflammatory drugs (NSAIDs). FI-302 showed potent analgesic and
antipyretic effects in the various models used. FI-302 showed an inhibitory effect
on acute inflammatory response such as carrageenin-induced edema, but did n ot show
any effect on chronic inflammatory response such as cotton pellet granuloma. These
effects of FI-302 were about 2 to 7 times more potent than those of non-a cidic
NSAIDs such as mepirizole and tiaramide. Moreover, FI-302 had little or no
ulcerogenic activity. From these results, it is conceivable that the spectrum of FI
-302's activities is similar to those of non-acidic NSAIDs such as mepirizole ana
tiaramide. These results suggest that FI-302 is a new non-ulcerogenic anti-inflamm
atory compound, and should be suitably applicable for clinical purposes. © 1985,
The Pharmaceutical Society of Japan. All rights reserved.
AU - Kameyama, T.
AU - Amanuma, F.
AU - Okuyama, S.
AU - Higuchi, S.
DB - Scopus
DO - 10.1248/bpb1978.8.477
IS - 6
KW - analgesic effect
anti-inflammatory effect
antipyretic effect
iV-(3-piperidinopropyl)-4-methyl-6-trifluoromethyl-furo[3,2- b]-indole-2-
carboxamide
ulcerogenic activity
Animals
Arthritis, Experimental
Capillary Permeability
Edema
Erythema
Granuloma
Guinea Pigs
Indoles
Lethal Dose 50
Male
Mice
Rats
Stomach Ulcer
4 methyl 6 trifluoromethylfuro[3,2 b]indole 2 [n (3 piperidinopropyl)]carboxamide
acetic acid
acetylcholine
adjuvant
aminophenazone
epirizole
flufenamic acid
histamine
ibuprofen
indometacin
mefenamic acid
new drug
phenylbutazone
phenylquinone
silver nitrate
tiaramide
unclassified drug
analgesia
animal experiment
animal model
article
biological model
dose response
drug comparison
drug efficacy
drug mechanism
drug response
drug screening
drug toxicity
fever
inflammation
intoxication
intradermal drug administration
intraperitoneal drug administration
mouse
nervous system
nonhuman
rat
soft tissue
stomach
subcutaneous drug administration
ulcerogenesis
yeast
M3 - Article
PY - 1985
SP - 477-486
ST - Pharmacological studies of furo [3,2- b] indole derivatives. i. analgesic,
anti-pyretic and antiinflammatory effects of fi-302, n- (3-piperidino-propyd-4-
methyl-6-trifluoromethyl-furo [3,2-b] indole-2-carboxamide, in experimental animals
T2 - Journal of Pharmacobio-Dynamics
TI - Pharmacological studies of furo [3,2- b] indole derivatives. i. analgesic,
anti-pyretic and antiinflammatory effects of fi-302, n- (3-piperidino-propyd-4-
methyl-6-trifluoromethyl-furo [3,2-b] indole-2-carboxamide, in experimental animals
0021796022&doi=10.1248%2fbpb1978.8.477&partnerID=40&md5=0b30f4dc0008e33a4e8c76a3e60
91ed6
VL - 8
ID - 5769
ER -
TY - JOUR
AB - Chronic inflammatory conditions can negatively impact intestinal barrier
function and affect the epithelium's interaction with nano-sized materials. We
demonstrate the application of a Caco-2/THP-1 co-culture mimicking the intestine in
healthy (i.e. stable) or inflamed state in nanotoxicological research. The co-
cultures were exposed to non-toxic concentrations of silver nanoparticles (AgNPs)
or silver nitrate (AgNO3) for 24 h. The barrier integrity and cytokine release as
well as necrotic and apoptotic cell death were investigated. AgNPs and AgNO3 most
strongly affected the inflamed co-culture. Higher concentrations of AgNPs induced a
significant increase in barrier integrity in the inflamed but not the stable co-
culture. Necrotic and apoptotic cell death was detected in both conditions but were
significantly more pronounced in the inflamed condition. The exposure to AgNO3
affected barrier integrity in all experimental set-ups, but caused nuclear
condensation only in the Caco-2 monoculture and the inflamed co-culture. AgNPs
reduced the release of monocyte chemoattractant protein-1 in the stable model.
Clear differences were observed in the effects of AgNPs and AgNO3 in relation to
the model's health status. The results suggest an increased vulnerability of the
inflamed epithelial barrier towards AgNPs underlining the importance to consider
the intestinal health status in the safety assessment of nanomaterials. © 2019 The
Authors
AU - Kämpfer, A. A. M.
AU - Urbán, P.
AU - La Spina, R.
AU - Jiménez, I. O.
AU - Kanase, N.
AU - Stone, V.
AU - Kinsner-Ovaskainen, A.
C7 - 104738
DB - Scopus
DO - 10.1016/j.tiv.2019.104738
KW - Caco-2
Intestinal in vitro co-culture
THP-1
Caco-2 Cells
Cytokines
Humans
Inflammation
Intestines
Metal Nanoparticles
Silver
Silver Nitrate
THP-1 Cells
silver nanoparticle
silver nitrate
cytokine
metal nanoparticle
silver
apoptosis
Article
Caco-2 cell line
cell death
coculture
controlled study
cytokine release
exposure
human
human cell
in vitro study
inflammation
nanoengineering
THP-1 cell line
toxic concentration
toxicity
intestine
metabolism
M3 - Article
PY - 2020
ST - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
Application of an in vitro co-culture model of the healthy and inflamed intestine
TI - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
85075892491&doi=10.1016%2fj.tiv.2019.104738&partnerID=40&md5=a1ac803ae6b6fb5dc5db12
b34c20c6d2
VL - 63
ID - 5298
ER -
TY - JOUR
AB - Chronic inflammatory conditions can negatively impact intestinal barrier
function and affect the epithelium's interaction with nano-sized materials. We
demonstrate the application of a Caco-2/THP-1 co-culture mimicking the intestine in
healthy (i.e. stable) or inflamed state in nanotoxicological research. The co-
cultures were exposed to non-toxic concentrations of silver nanoparticles (AgNPs)
or silver nitrate (AgNO3) for 24 h. The barrier integrity and cytokine release as
well as necrotic and apoptotic cell death were investigated. AgNPs and AgNO3 most
strongly affected the inflamed co-culture. Higher concentrations of AgNPs induced a
significant increase in barrier integrity in the inflamed but not the stable co-
culture. Necrotic and apoptotic cell death was detected in both conditions but were
significantly more pronounced in the inflamed condition. The exposure to AgNO3
affected barrier integrity in all experimental set-ups, but caused nuclear
condensation only in the Caco-2 monoculture and the inflamed co-culture. AgNPs
reduced the release of monocyte chemoattractant protein-1 in the stable model.
Clear differences were observed in the effects of AgNPs and AgNO3 in relation to
the model's health status. The results suggest an increased vulnerability of the
inflamed epithelial barrier towards AgNPs underlining the importance to consider
the intestinal health status in the safety assessment of nanomaterials.
AN - WOS:000509613100017
AU - Kampfer, A. A. M.
AU - Urban, P.
AU - La Spina, R.
AU - Jimenez, I. O.
AU - Kanase, N.
AU - Stone, V.
AU - Kinsner-Ovaskainen, A.
C7 - 104738
DA - MAR
DO - 10.1016/j.tiv.2019.104738
PY - 2020
SN - 0887-2333
ST - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
TI - Ongoing inflammation enhances the toxicity of engineered nanomaterials:
VL - 63
ID - 6160
ER -
TY - JOUR
AB - This research article investigates the one-pot synthesis of gold and silver
chloride nanoparticles functionalized by fruit extract of Crataegus pinnatifida as
reducing and stabilizing agents and their possible roles as novel anti-inflammatory
agents. Hawthorn (C. pinnatifida) fruits are increasingly popular as raw materials
for functional foods and anti-inflammatory potential agents because of abundant
flavonoids. The reduction of auric chloride and silver nitrate by the aqueous fruit
extract led to the formation of gold and silver chloride nanoparticles. The
nanoparticles were further characterized by field emission transmission electron
microscopy indicated that CP-AuNps and CP-AgClNps were hexagonal and cubic shape,
respectively. According to X-ray diffraction results, the average crystallite sizes
of CP-AuNps and CP-AgClNps were 14.20 nm and 24.80 nm. The biosynthesized CP-
AgClNps served as efficient antimicrobial agents against Escherichia coli and
Staphylococcus aureus. Furthermore, CP-AuNps and CP-AgClNps enhanced the DPPH
radical scavenging activity of the fruit extract. Lastly, MTT assay of
nanoparticles demonstrated low toxicity in murine macrophage (RAW264.7).
Biosynthesized nanoparticles also reduced the production of the inflammatory
cytokines including nitric oxide and prostaglandin E2 in lipopolysaccharide-induced
RAW264.7 cells. Altogether, these findings suggest that CP-AuNps and CP-AgClNps can
be used as novel drug carriers or biosensors with intrinsic anti-inflammatory
activity. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.
AU - Kang, J. P.
AU - Kim, Y. J.
AU - Singh, P.
AU - Huo, Y.
AU - Soshnikova, V.
AU - Markus, J.
AU - Ahn, S.
AU - Chokkalingam, M.
AU - Lee, H. A.
AU - Yang, D. C.
DB - Scopus
DO - 10.1080/21691401.2017.1376674
IS - 8
KW - anti-inflammatory agents
Crataegus pinnatifida
gold nanoparticles
silver chloride nanoparticles
Animals
Crataegus
Escherichia coli
Fruit
Gold
Mice
Nanoparticles
Plant Extracts
RAW 264.7 Cells
Silver Compounds
Biochemistry
Chlorine compounds
Drug delivery
Free radicals
Fruits
Gold compounds
Gold nanoparticles
Nitric oxide
Silver halides
X ray diffraction
antibiotic agent
antioxidant
crataegus pinnatifida fruit extract
gallic acid
gold chloride
gold chloride nanoparticle
gold nanoparticle
neomycin
nitric oxide
plant extract
prostaglandin E2
silver chloride
silver chloride nanoparticle
silver nanoparticle
unclassified drug
antiinfective agent
gold
nanoparticle
silver derivative
animal cell
animal experiment
Article
bacterial strain
controlled study
crystallization
cytokine production
drug synthesis
in vitro study
mouse
nanofabrication
nonhuman
one pot synthesis
RAW 264.7 cell line
animal
chemistry
fruit
M3 - Article
PY - 2018
SP - 1530-1540
ST - Biosynthesis of gold and silver chloride nanoparticles mediated by Crataegus
pinnatifida fruit extract: in vitro study of anti-inflammatory activities
T2 - Artificial Cells, Nanomedicine and Biotechnology
TI - Biosynthesis of gold and silver chloride nanoparticles mediated by Crataegus
pinnatifida fruit extract: in vitro study of anti-inflammatory activities
85029587177&doi=10.1080%2f21691401.2017.1376674&partnerID=40&md5=73549b75e21f1e0fec
4e252e2b8cfdcf
VL - 46
ID - 5414
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are widely used nanoparticles and they are
mainly used in antibacterial and personal care products. In this study, we
evaluated the effect of AgNPs on cell death induction in the murine dendritic cell
line DC2.4. DC2.4 cells exposed to AgNPs showed a marked decrease in cell viability
and an induction of lactate dehydrogenase (LDH) leakage in a time- and dose-
dependent manner. In addition, AgNPs promoted reactive oxygen species (ROS)-
dependent apoptosis and AgNP-induced ROS triggered a decrease in mitochondrial
membrane potential. The activation of the intracellular signal transduction pathway
was also observed in cells cultured with AgNPs. Taken together, our data
demonstrate that AgNPs are able to induce a cytotoxic effect in DCs through ROS
generation. This study provides important information about the safety of AgNPs
that may help in guiding the development of nanotechnology applications.
AN - WOS:000307680000006
AU - Kang, K.
AU - Jung, H.
AU - Lim, J. S.
DA - JUL 31
DO - 10.4062/biomolther.2012.20.4.399
IS - 4
PY - 2012
SN - 1976-9148
2005-4483
SP - 399-405
ST - Cell Death by Polyvinylpyrrolidine-Coated Silver Nanoparticles is Mediated by
ROS-Dependent Signaling
T2 - BIOMOLECULES & THERAPEUTICS
TI - Cell Death by Polyvinylpyrrolidine-Coated Silver Nanoparticles is Mediated by
ROS-Dependent Signaling
VL - 20
ID - 5943
ER -
TY - JOUR
AB - Nanocrystalline silver (Ag) and Ag containing nanostructure synthesized using
various methods have been studied for their antimicrobial, wound healing, and anti-
inflammatory efficacy. Among these, crystalline silver chloride (AgCl)
nanostructures exhibit desirable properties for biological and biomedical
applications. However, most of them are synthesized using hazardous agents and
organic solvents, which has been limited for application in the biological field. A
simple and environmentally friendly method was demonstrated for AgCl nanoparticles
stabilized with chitosan oligomer (CHI-AgCl NPs) as both a resource of Cl ions and
stabilizing agent with expectations of synergistic effects. The CHI-AgCl NPs
stabilized by the chitosan oligomer had spherical morphology with a mean diameter
of 42 ± 15 nm. Ag ions precipitated as AgCl in presence of Cl ions, which remained
in the protonated amine group after HCl hydrolysis of the chitosan. Moreover, much
of the amine and hydroxyl group bound to the AgCl NPs for growth and stabilization.
These nanoparticles were characterized via various spectroscopic techniques,
including UV–Vis spectrophotometry, X-ray photoelectron spectrometry, X-ray
diffractometry, and transmission electron microscopy. © 2014, Springer
Science+Business Media New York.
AU - Kang, Y. O.
AU - Lee, T. S.
AU - Park, W. H.
DB - Scopus
DO - 10.1007/s10856-014-5294-1
IS - 12
KW - Anti-Infective Agents
Apoptosis
Bacterial Physiological Phenomena
Cell Survival
Chitosan
Excipients
Materials Testing
Metal Nanoparticles
Particle Size
Silver Compounds
Surface Properties
Biohazards
Chlorine
Chlorine compounds
Green Synthesis
Ions
Microorganisms
Nanocrystals
Nanoparticles
Oligomers
Tissue regeneration
X ray diffraction analysis
X rays
antiinfective agent
chitosan
chloride ion
hydroxyl group
nanoparticle
oligomer
silver chloride
silver chloride nanoparticle
unclassified drug
excipient
metal nanoparticle
silver derivative
Spherical morphologies
VIS spectrophotometry
X-ray photoelectron spectrometries
absorption
Article
controlled study
decomposition
drug synthesis
Escherichia coli
green chemistry
hydrolysis
nonhuman
proton transport
reaction time
spectrophotometry
thermostability
X ray photoelectron spectroscopy
apoptosis
bacterial phenomena and functions
cell survival
chemistry
dose response
drug effects
materials testing
particle size
physiology
procedures
surface property
synthesis
ultrastructure
Silver halides
M3 - Article
PY - 2014
SP - 2629-2638
ST - Green synthesis and antimicrobial activity of silver chloride nanoparticles
stabilized with chitosan oligomer
TI - Green synthesis and antimicrobial activity of silver chloride nanoparticles
stabilized with chitosan oligomer
84912151045&doi=10.1007%2fs10856-014-5294-
1&partnerID=40&md5=36422bd3023f57ccf5d49e275c6667aa
VL - 25
ID - 5649
ER -
TY - JOUR
AB - Aim: To determine the flow characteristics and subcutaneous tissue reactions
to five endodontic sealers. Methodology: The materials used were Procosol, AH26,
Endomethasone, Sealapex and Endion. The sealers were prepared following the
manufacturers' instructions, and 0.075 mL of each material was placed on a glass
surface, which was then rotated 90°. The samples were stored at 37°C and 95%
humidity. The displacement of the sealer was recorded by measuring the difference
between its original position and the position recorded at 15 and 60 min. Three
samples of each material were used. Two pockets were created in the back of Wistar
rats, and one silicone tube, 1 mm in diameter and 1 cm in length, was implanted in
each. One was filled with one of the materials under study, and the other empty
tube was implanted as a control. Fourteen days after implantation, the animals were
sacrificed, and samples of the skin containing the tubes were histologically
processed. Histological and histomorphometric evaluations of the tissues adjacent
to the open end of the tube were carried out. The volume of tissue reaction was
measured histomorphometrically according to standard stereological principles.
Results were statistically analysed using analysis of variance and Duncan's test.
Results: The highest flow values were obtained with Sealapex and AH26. Time
significantly affected the flow and the material (P < 0.001). Procosol and Endion
produced the most severe histological reactions; these were outlined by fibrous
tissue; AH26, Endomethasone and Sealapex produced reactions of smaller size and
with more moderately defined limits. Conclusions: The flow did not correlate with
the degree of inflammatory response. Procosol and Endion produced the most severe
tissue reactions, whereas Endomethasone, Sealapex and AH26 produced only minimum
reactions.
AU - Kaplan, A. E.
AU - Ormaechea, M. F.
AU - Picca, M.
AU - Canzobre, M. C.
AU - Ubios, A. M.
DB - Scopus
DO - 10.1046/j.1365-2591.2003.00683.x
IS - 8
Dental materials
Endodontics
Flow
Animals
Bismuth
Calcium Hydroxide
Dexamethasone
Drug Combinations
Epoxy Resins
Fibroblasts
Formaldehyde
Hydrocortisone
Materials Testing
Rats
Rats, Wistar
Resins, Synthetic
Rheology
Salicylates
Silver
Skin
Thymol
Titanium
Viscosity
Zinc Oxide
calcium hydroxide
epoxy resin
glass ionomer
sealant
steroid
zinc oxide eugenol
animal experiment
animal tissue
article
biocompatibility
controlled study
endodontics
flow kinetics
histology
humidity
morphometrics
nonhuman
rat
rat strain
silastic tube
skin
stereometry
storage temperature
subcutaneous tissue
tissue reaction
M3 - Article
PY - 2003
SP - 527-532
ST - Rheological properties and biocompatibility of endodontic sealers
TI - Rheological properties and biocompatibility of endodontic sealers
0141954001&doi=10.1046%2fj.1365-
2591.2003.00683.x&partnerID=40&md5=55a29abfe43b4ce78ff52e519bf4362a
VL - 36
ID - 5812
ER -
TY - JOUR
AB - Introduction: An ideal wound dressing material needs to be predisposed with
desirable attributes like anti-infective effect, skin hydration balance, adequate
porosity and elasticity, high mechanical strength, low wound surface adherence, and
enhanced tissue regeneration capability. In this work, we have synthesized
hydrogel-based wound patches having antibacterial silver nanoparticles and
antioxidant epigallocatechin gallate (EGCG) and showed fast wound closure through
their synergistic interaction without any inherent toxicity. Methods and results:
Wound patches were synthesized from modified guar gum polymer and assessed to
determine accelerated wound healing. The modified polymer beget chemical-free in-
situ synthesis of monodispersed silver NPs (~12 nm), an antimicrobial agent,
besides lending ionic surface charges. EGCG impregnated during ionotropic gelation
process amplified the efficacy of wound patches that possess apt tensile strength,
porosity, and swellability for absorbing wound exudates. Further, in vitro studies
endorsed them as non-cytotoxic and the post agent effect following exposure to the
patch showed an unbiased response to E coli K12 and B. subtilis. In vivo study
using sub-cutaneous wounds in Wistar rats validated its accelerated healing
properties when compared to a commercially available wound dressing material (skin
graft; Neuskin-F®) through better wound contraction, promoted collagen deposition
and enhanced vascularization of wound region by modulating growth factors and
inflammatory cytokines. Conclusion: Synthesized wound patches showed all the
desired attributes of a clinically effective dressing material and the results were
validated in various in vitro and in vivo assays. © 2019 Kar et al.
AU - Kar, A. K.
AU - Singh, A.
AU - Dhiman, N.
AU - Purohit, M. P.
AU - Jagdale, P.
AU - Kamthan, M.
AU - Singh, D.
AU - Kumar, M.
AU - Ghosh, D.
AU - Patnaik, S.
DB - Scopus
DO - 10.2147/IJN.S228462
KW - Aminated guar gum
Epigallocatechin gallate
Hydrogels
Wound healing
Animals
Antioxidants
Bacillus subtilis
Bandages
Catechin
Escherichia coli
Galactans
Mannans
Metal Nanoparticles
Plant Gums
Polymers
Rats, Wistar
Silver
Tensile Strength
Wound Healing
calcium
carbon
creatinine
gamma interferon
interleukin 10
interleukin 6
Ki 67 antigen
oxygen
silver nanoparticle
sodium
urea
vasculotropin
antiinfective agent
antioxidant
catechin
galactan
guar gum
mannan
metal nanoparticle
plant gum
polymer
silver
animal experiment
animal model
animal tissue
antigen expression
Article
blood clotting time
cell proliferation
collagen synthesis
controlled study
dispersity
epithelization
Escherichia coli K 12
facile green synthesis
granulation tissue
hydrogel
in vitro study
in vivo study
neovascularization (pathology)
nonhuman
particle size
physical parameters
porosity
protein expression
Raman spectrometry
rat
skin injury
sustained drug release
swellability
synthesis
tensile strength
urea blood level
vascularization
Wistar rat
wound closure
wound contraction
wound fluid
wound healing
wound patch
X ray diffraction
zeta potential
animal
bandage
chemistry
drug effect
M3 - Article
PY - 2019
SP - 9837-9854
ST - Polymer-assisted in situ synthesis of silver nanoparticles with
epigallocatechin gallate (EGCG) impregnated wound patch potentiate controlled
inflammatory responses for brisk wound healing
TI - Polymer-assisted in situ synthesis of silver nanoparticles with
epigallocatechin gallate (EGCG) impregnated wound patch potentiate controlled
inflammatory responses for brisk wound healing
85076726436&doi=10.2147%2fIJN.S228462&partnerID=40&md5=01e1ca82e16287d615a830fb24ab
1f11
VL - 14
ID - 5449
ER -
TY - JOUR
AB - Development of novel antidiabetic agents using various organic compounds and
biomolecules has been in practice for a longtime. Recently, nanomaterials are also
being used in antidiabetic studies for their unique properties such as small size,
biocompatibility and ability to penetrate cell membrane for carrying drugs. Herein,
in vivo antidiabetic activity of gold nanoparticles (AuNPs) synthesized using the
antidiabetic potent plant Gymnema sylvestre R. Br on wistar albino rats has been
evaluated. The formation of AuNPs and their morphology were confirmed using
spectroscopic and microscopic analyses, respectively. The treatment of AuNPs has
shown significant reduction in blood glucose level on diabetic rats. AuNPs were
also tested for its anti-inflammatory effect by estimating the serum levels of
tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and high-sensitive C-
reactive protein (CRP). (C) 2014 Elsevier B.V. All rights reserved.
AN - WOS:000343612900063
AU - Karthick, V.
AU - Kumar, V. G.
AU - Dhas, T. S.
AU - Singaravelu, G.
AU - Sadiq, A. M.
AU - Govindaraju, K.
DA - OCT 1
DO - 10.1016/j.colsurfb.2014.07.022
PY - 2014
SN - 0927-7765
1873-4367
SP - 505-511
ST - Effect of biologically synthesized gold nanoparticles on alloxan-induced
diabetic rats-An in vivo approach
TI - Effect of biologically synthesized gold nanoparticles on alloxan-induced
diabetic rats-An in vivo approach
VL - 122
ID - 6542
ER -
TY - JOUR
AB - Nanoparticles (NPs) are currently being investigated along with the use of
biodegradable polymer containing active agents in many areas of medicine for
targeted applications. The present study was aimed to synthesize novel compound
Benzodioxane midst piperazine (BP) and characterization of a BP decorated chitosan
silver nanoparticles (BP*C@AgNPs) and shown effective against hazardous pathogens,
and also having anti-inflammatory property. It was further evaluated for molecular
docking proofs, and toxicity. The BP*C@AgNPs had spherical shape with size of 36.6
nm with wide biocidal activity against hazardous Gram-positive and Gram-negative
bacteria with excellent inhibition at 100 μg/mL for S. aureus (10.08 ± 0.05 mm
ZOI), and E. coli (10.03 ± 0.04 mm ZOI) compared to antibiotic Streptomycin. The
anti-inflammatory activity exhibited IC50 value of 71.61 ± 1.05 μg/mL for
BP*C@AgNPs compared to indomethacin (IC50 = 40.15 ± 1.21 μg/mL). Also, the docking
study of BP showed excellent score for COX1 and DNA gyrase. This in silico study
confirmed the achieved efficacy of BP, with less toxicity against normal PMBCs in
vitro and in vivo studies. This study concludes that, the novel synthesized
BP*C@AgNPs had excellent biocidal property and as anti-inflammatory candidate
revealed by docking studies, it confirms BP*C@AgNPs for first-class therapeutic
applications in the area of medicinal nanotechnology for the coming days. © 2017
AU - Karthik, C. S.
AU - Manukumar, H. M.
AU - Ananda, A. P.
AU - Nagashree, S.
AU - Rakesh, K. P.
AU - Mallesha, L.
AU - Qin, H. L.
AU - Umesha, S.
AU - Mallu, P.
AU - Krishnamurthy, N. B.
DB - Scopus
DO - 10.1016/j.ijbiomac.2017.12.045
Chitosan
Molecular-docking
Piperazine
Toxicity
Animals
Bacteria
Metal Nanoparticles
Mice
Molecular Conformation
Molecular Dynamics Simulation
Oxidative Stress
Piperazines
Silver
X-Ray Diffraction
benzodiazepine
chitosan
gyrase inhibitor
indometacin
piperazine
silver nanoparticle
streptomycin
stromal cell derived factor 1
unclassified drug
[4(4 methoxybenzoyl)piperazin 1 yl] (2,3-dihydrobenzo[b][1,4]dioxin 3 yl)methanone
antiinfective agent
metal nanoparticle
piperazine derivative
silver
animal cell
animal experiment
Article
Bacillus cereus
biosafety
computer model
controlled study
cytotoxicity
drug efficacy
drug synthesis
Escherichia coli
IC50
in vitro study
in vivo study
molecular docking
mouse
nanomedicine
nonhuman
particle size
Salmonella enterica serovar Typhimurium
Shigella flexneri
animal
bacterium
chemistry
conformation
drug effect
molecular dynamics
oxidative stress
ultrastructure
X ray diffraction
M3 - Article
PY - 2018
SP - 489-502
ST - Synthesis of novel benzodioxane midst piperazine moiety decorated chitosan
silver nanoparticle against biohazard pathogens and as potential anti-inflammatory
candidate: A molecular docking studies
TI - Synthesis of novel benzodioxane midst piperazine moiety decorated chitosan
silver nanoparticle against biohazard pathogens and as potential anti-inflammatory
candidate: A molecular docking studies
85037647743&doi=10.1016%2fj.ijbiomac.2017.12.045&partnerID=40&md5=e8c1c8987e33ad732
f6dd912d619301a
VL - 108
ID - 5459
ER -
TY - JOUR
AB - Natural products are important leads in drug discovery. The search for
effective plant-derived agents or their synthetic analogues has continued to be of
interest to biologists and chemists for a long time. Herein, we have synthesized a
novel compound, P1C, and P1C-Tit*CAgNPs from chitosan; P1C is a precursor and an
anti-inflammatory candidate, which has been validated by molecular docking studies.
The synthesized P1C-Tit*CAgNPs showed monodisperse, spherical, and cationic nature
and antioxidant properties, protecting destabilization of the erythrocyte membrane
by the azo compound 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH); the
involvement of NPs as a protective agent for biomolecules, such as DNA and protein,
followed by the treatment of NPs with AAPH was confirmed. The inhibition of
cellular damage and leakage of cellular inflammatory agents was confirmed by AFM,
SEM, TEM, SDS-PAGE, LDH, and PLA2 enzyme inhibition via in vitro studies. The anti-
inflammatory property of P1C was further validated by in silico molecular docking
studies and showed that, the P1C best pose aligned to PLA2 compared to standard
drug. The significant anticancer property of P1C-Tit*CAgNPs was confirmed against
MCF7, U373, and C6 cancer cell lines. Thus, the present study highlights the
synthesized P1C in P1C-Tit*CAgNPs as a target PLA2-specific anti-inflammatory
candidate, and further tuning of small and development-functionalized nanoparticles
has a great future in medicine; hence, their clinical applications are warranted.
AN - WOS:000435859400012
AU - Karthik, C. S.
AU - Manukumar, H. M.
AU - Sandeep, S.
AU - Sudarshan, B. L.
AU - Nagashree, S.
AU - Mallesha, L.
AU - Rakesh, K. P.
AU - Sanjay, K. R.
AU - Mallu, P.
AU - Qin, H. L.
DA - APR 1
DO - 10.1039/c7md00628d
IS - 4
PY - 2018
SN - 2040-2503
2040-2511
SP - 713-724
ST - Development of piperazine-1-carbothioamide chitosan silver nanoparticles
(P1C-Tit*CAgNPs) as a promising anti-inflammatory candidate: a molecular docking
validation
T2 - MEDCHEMCOMM
TI - Development of piperazine-1-carbothioamide chitosan silver nanoparticles
(P1C-Tit*CAgNPs) as a promising anti-inflammatory candidate: a molecular docking
validation
VL - 9
ID - 6072
ER -
TY - JOUR
AB - Despite advances in our understanding of the oxidative pathways mediated by
free hemoglobin (Hb), the precise contribution of its highly reactive redox forms
to tissue and organ toxicities remains ambiguous. Heme, a key degradation byproduct
of Hb oxidation, has recently been recognized as a damage-associated molecular
pattern (DAMP) molecule, able to trigger inflammatory responses. Equally damaging
is the interaction of the highly redox active forms of Hb with other biological
molecules. We determined the kinetics of heme loss from individual Hb redox states-
ferrous (Fe2+), ferric (Fe3+), and ferryl (Fe4+)-using two different heme receptor
proteins: hemopexin (Hxp), a naturally occurring heme scavenger in plasma, and a
double mutant (H64Y/V86F), apomyoglobin (ApoMb), which avidly binds heme released
from Hb. We show for the first time that ferric Hb (Fe3+) loses heme at rates
substantially higher than that of ferryl Hb (Fe4+). This was also supported by a
higher expression of heme oxygenase-1 (HO-1) when ferric Hb was added to cultured
lung alveolar epithelial cells (E10). The reported cytotoxicity of Hb may therefore
be attributed to a combination of accelerated heme loss from the ferric form and
protein radical formation associated with ferryl Hb. Targeted therapeutic
interventions can therefore be designed to curb specific oxidative pathways of Hb
in hemolytic anemias and when Hb is used as an oxygen-carrying therapeutic.
AN - WOS:000383618300001
AU - Kassa, T.
AU - Jana, S.
AU - Meng, F.
AU - Alayash, A. I.
DA - SEP
DO - 10.1002/2211-5463.12103
IS - 9
PY - 2016
SN - 2211-5463
SP - 876-884
ST - Differential heme release from various hemoglobin redox states and the
upregulation of cellular heme oxygenase-1
T2 - FEBS OPEN BIO
TI - Differential heme release from various hemoglobin redox states and the
upregulation of cellular heme oxygenase-1
VL - 6
ID - 6671
ER -
TY - JOUR
AB - Silver is known to have powerful antibacterial properties against a variety
of micro-organisms and has a low toxicity and a favorable biocompatibility profile.
This study was designed to evaluate the effectiveness of silver-coated catheters in
preventing early exit-site infection and to assess tunnel morphology. Seven male
Sprague-Dawley rats underwent simultaneous implantation of two double-cuffed,
silver-coated silicone rubber and standard silicone rubber catheters. Weekly
observations and photographs documented exit-site characteristics. The animals were
sacrificed and catheters removed and processed for histopathology of the external
tunnel at 5 weeks. Exit sites of silver-coated catheters tended to have less
inflammation and infection and healed better than those of uncoated catheters;
however, these data did not achieve significance using the Wilcoxon signed-rank
test. Sections of the external tunnel of well-healing exit sites showed an
epithelialized tract with granulation tissue near the cuff and significant invasion
of the external cuff by collagen with a mild neutrophilic inflammatory response. In
contrast, the histology of the external tunnel of infected exists revealed exudate
overlying inflammatory granulation tissue and a variable degree of fibrosis of the
cuff. When the exit sites appeared similar, no significant histopathological
differences in sinus tract and cuff morphology were noted with either silver or
standard catheters. In conclusion, these findings suggest that silver coating of
catheters may decrease the incidence of early exit-site infections and allow better
ingrowth of the catheter.
AU - Kathuria, P.
AU - Moore, H. L.
AU - Prowant, B. F.
AU - Khanna, R.
AU - Twardowski, Z. J.
DB - Scopus
KW - Animals
Catheters, Indwelling
Male
Peritoneal Cavity
Peritoneal Dialysis
Rats
Silver
Surface Properties
Wound Healing
silver
animal
article
indwelling catheter
instrumentation
male
microbiology
peritoneal cavity
peritoneal dialysis
rat
Sprague Dawley rat
surface property
wound healing
M3 - Article
PY - 1995
SP - 189-192
ST - Preliminary evaluation of silver-coated peritoneal catheters in rats
T2 - Advances in peritoneal dialysis. Conference on Peritoneal Dialysis
TI - Preliminary evaluation of silver-coated peritoneal catheters in rats
0029449422&partnerID=40&md5=536103383c7de2e9319bd90605f2920a
VL - 11
ID - 5836
ER -
TY - JOUR
AB - Apoptosis induction is a common therapeutic approach. However, many cancer
cells are resistant to apoptotic death and alternative cell death pathways
including pyroptosis and necroptosis need to be triggered. At the same time, danger
signals that include HMGB1 and HSP70 can be secreted/released by damaged cancer
cells that boost antitumor immunity. We studied the cytotoxic effects of AgAu NPs,
Ag NPs and Au NPs with regard to the programmed cell death (apoptosis, necroptosis,
pyroptosis) and the secretion/release of HSP70 and HMGB1. Cancer cell lines were
incubated with 30, 40 and 50 µg/mL of AgAu NPs, Ag NPs and Au NPs. Cytotoxicity was
estimated using the MTS assay, and mRNA fold change of CASP1, CASP3, BCL-2, ZPB1,
HMGB1, HSP70, CXCL8, CSF1, CCL20, NLRP3, IL-1β and IL-18 was used to investigate
the associated programmed cell death. Extracellular levels of HMGB1 and IL-1β were
investigated using the ELISA technique. The nanoparticles showed a dose dependent
toxicity. Pyroptosis was triggered for LNCaP and MDA-MB-231 cells, and necroptosis
for MDA-MB-231 cells. HCT116 cells experience apoptotic death and show increased
levels of extracellular HMGB1. Our results suggest that in a manner dependent of
the cellular microenvironment, AgAu NPs trigger mixed programmed cell death in P53
deficient MDA-MB-231 cells, while they also trigger IL-1β release in MDA-MB-231 and
LNCaP cells and release of HMGB1 in HCT116 cells. © 2022 by the authors. Licensee
AU - Katifelis, H.
AU - Nikou, M. P.
AU - Mukha, I.
AU - Vityuk, N.
AU - Lagopati, N.
AU - Piperi, C.
AU - Farooqi, A. A.
AU - Pippa, N.
AU - Efstathopoulos, E. P.
AU - Gazouli, M.
C7 - 1546
DB - Scopus
DO - 10.3390/cancers14061546
IS - 6
KW - Apoptosis
Bimetallic nanoparticles
Gold
Necroptosis
Pyroptosis
Silver
caspase 3
complementary DNA
cryopyrin
gold nanoparticle
interleukin 18
interleukin 1beta
interleukin 8
messenger RNA
protein bcl 2
protein p53
silver nanoparticle
tryptophan
zinc binding protein
apoptosis
Article
cancer cell
cancer cell line
cell culture
cell death
cell line
cell viability
cytotoxicity
fibroblast
GBM2 cell line
gene expression
HCT 116 cell line
HEK293 cell line
human
human cell
immunity
inflammation
Li-Fraumeni syndrome
LNCaP C4-2B cell line
LNCaP cell line
malignant neoplasm
MCF-7 cell line
MTS assay
necroptosis
pyroptosis
RNA extraction
M3 - Article
PY - 2022
ST - Ag/Au Bimetallic Nanoparticles Trigger Different Cell Death Pathways and
Affect Damage Associated Molecular Pattern Release in Human Cell Lines
T2 - Cancers
TI - Ag/Au Bimetallic Nanoparticles Trigger Different Cell Death Pathways and
Affect Damage Associated Molecular Pattern Release in Human Cell Lines
85126549212&doi=10.3390%2fcancers14061546&partnerID=40&md5=f1004ede8b4bd9acea12b5b2
90530a36
VL - 14
ID - 5105
ER -
TY - JOUR
AB - We previously reported on the short-term biocompatibility of a reconstituted
type-I collagen prosthesis that had been tested in the Achilles tendons of rabbits.
Preliminary results indicated that, by ten weeks after implantation, carbodiimide-
cross-linked implants had been replaced by neotendon in a manner that was similar
to that of autogenous tendon grafts that had been used as controls. Also by ten
weeks after implantation, glutaraldehyde-cross-linked collagen implants were
encapsulated and appeared to have caused an acute inflammatory response. In the
present study, carbodiimide and glutaraldehyde-cross-linked collagen implants and
autogenous grafts that served as controls were implanted for fifty-two weeks as a
replacement for a three-centimeter section of the Achilles tendon of rabbits. The
absence of a crimp in a cross-linked implant and the presence of a crimp in normal
tendon and in tendon that formed after an implant had been resorbed made it
possible to distinguish between a cross-linked implant and new host tendon that had
replaced the implant after it was resorbed. New collagen that had replaced the
implant and autogenous (control) tendon graft were compared with normal Achilles
tendon with respect to the angle and length of the crimp. The autogenous grafts and
the carbodiimide-cross-linked collagen implants had been completely resorbed and
replaced by neotendon. The neotendon that was present fifty-two weeks after
implantation was similar, but not identical, to normal tendon. In contrast, the
glutaraldehyde-cross-linked implant was essentially inert, had not been resorbed,
and was surrounded by a capsule of collagenous connective tissue. The neotendon in
the capsule was also similar, but not identical, to normal tendon. There were more
cells in the capsule than in the autogenous grafts or in the carbodiimide-cross-
linked implants. The results of the present study indicate that rapid repair is
achieved with a carbodiimide-cross-linked collagenous implant that has a structure
and mechanical properties that are similar to those of an autogenous tendon graft
and that biodegrades at a similar rate. Prolonged biodegradation of a
glutaraldehyde-cross-linked collagenous implant results in formation of a capsule
and only limited formation of neotendon.
AN - WOS:A1991FH23600013
AU - Kato, Y. P.
AU - Dunn, M. G.
AU - Zawadsky, J. P.
AU - Tria, A. J.
AU - Silver, F. H.
DA - APR
DO - 10.2106/00004623-199173040-00013
IS - 4
PY - 1991
SN - 0021-9355
1535-1386
SP - 561-574
ST - REGENERATION OF ACHILLES-TENDON WITH A COLLAGEN TENDON PROSTHESIS - RESULTS
OF A ONE-YEAR IMPLANTATION STUDY
T2 - JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
TI - REGENERATION OF ACHILLES-TENDON WITH A COLLAGEN TENDON PROSTHESIS - RESULTS
OF A ONE-YEAR IMPLANTATION STUDY
VL - 73A
ID - 6807
ER -
TY - JOUR
AB - The hydrazones of nonsteroidal anti-inflammatory drugs (NSAIDs) diclofenac
and ibuprofen are synthesized with aldehydes of pyridine and imidazole and are
characterized by H-1, C-13 and mass spectroscopy. Cu(II) complexes of hydrazones
constructed from these ligands possess square planar geometry for bidentate
diclofenac-hydrazone and tridentate ibuprofen-hydrazone conjugates with [Cu(L)(2)]
and [Cu(L)Cl] compositions, respectively. The observed irreversible Cu(II)/Cu(I)
redox couple in the range of +0.20 to +0.61 V is due to the substantial distortion
in the square-planar geometry. ESR studies indicate the appreciably covalent
character within M-L bonding due to extensive delocalization of electron from d (x)
(2) (--) (y) (2) orbital. The hydrazone-NSAID conjugates exhibit substantial
cytotoxicity against A-549, HCT-116 and MDA-MB-231 cancer cell lines with
ibuprofen-imidazole-hydrazone ligand possessing the lowest IC50 (2.26 mu M) amongst
the synthesized NSAID-conjugates. Interestingly, its Cu(II) complex also displays
excellent anticancer activity against MDA-MB- 231 with IC50 value of 3.58 mu M.
Such a feature may be ascribed to the synergistic association of Cu(II)-NSAID-
hydrazone linkage. Thus, conjugation of NSAID with hydrazone and its complexation
with a bioactive metal ion may be regarded as a potential strategy for designing of
non-platinum anticancer agents.
AN - WOS:000588802100001
AU - Kaur, J.
AU - Chikate, T.
AU - Bandyopadhyay, P.
AU - Basu, S.
AU - Chikate, R.
C6 - NOV 2020
DA - NOV 11
DO - 10.1080/00958972.2020.1843160
IS - 23
PY - 2020
SN - 0095-8972
1029-0389
SP - 3186-3202
ST - Cu(II) complexes of hydrazones-NSAID conjugates: synthesis, characterization
and anticancer activity
T2 - JOURNAL OF COORDINATION CHEMISTRY
TI - Cu(II) complexes of hydrazones-NSAID conjugates: synthesis, characterization
and anticancer activity
VL - 73
ID - 6696
ER -
TY - CONF
AU - Kawamori, T.
AU - Nakatsugi, S.
AU - Ohta, T.
AU - Sugimura, T.
AU - Wakabayashi, K.
DB - Scopus
DO - 10.1007/978-1-4615-0193-0_57
KW - Animals
Anticarcinogenic Agents
Carcinogens
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Cyclooxygenase Inhibitors
Female
Imidazoles
Isoenzymes
Mammary Neoplasms, Animal
Prostaglandin-Endoperoxide Synthases
Rats
Sulfonamides
Animalia
2 amino 1 methyl 6 phenylimidazo[4,5 b]pyridine
cytokine
DNA fragment
growth factor
lipopolysaccharide
nimesulide
prostaglandin E2
2 amino 1 methyl 6 phenylimidazo(4,5 b)pyridine
2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
carcinogen
cyclooxygenase 2
imidazole derivative
isoenzyme
prostaglandin synthase inhibitor
sulfonamide
animal experiment
animal model
animal tissue
apoptosis
body weight
breast carcinogenesis
breast carcinoma
cell proliferation
chemoprophylaxis
conference paper
controlled study
drug mechanism
drug selectivity
enzyme activity
enzyme induction
female
fibroblast
hormone determination
inflammation
macrophage
mitogenesis
nonhuman
priority journal
protein expression
protein function
rat
silver staining
tumor growth
Western blotting
animal
article
drug effect
experimental neoplasm
metabolism
pathology
PY - 2002
SP - 371-376
ST - Chemopreventive effects of nimesulide, a selective cyclooxygenase-2
inhibitor, against PHIP-induced mammary carcinogenesis
T2 - Advances in Experimental Medicine and Biology
TI - Chemopreventive effects of nimesulide, a selective cyclooxygenase-2
inhibitor, against PHIP-induced mammary carcinogenesis
0142226855&doi=10.1007%2f978-1-4615-0193-
0_57&partnerID=40&md5=0c5e44b59de0a0b61e5199d3d8ee2556
VL - 507
ID - 5787
ER -
TY - JOUR
AB - The interest in the application of plant extracts as modifiers of polymers
intended for biomedical purposes is constantly increasing. The therapeutical
properties of the licorice root, including its anti-inflammatory and antibacterial
activity, make this plant particularly promising. The same applies to silver
nanoparticles showing antibacterial properties. Thus the main purpose of the
research was to design hydrogel dressings containing both licorice root extract and
nanosilver so as to obtain a system promoting wound regeneration processes by
preventing infection and inflammation within the wound. The first step included the
preparation of the plant extract via the solid-liquid extraction using the Soxhlet
extractor and the synthesis of silver nanoparticles by the chemical reduction of
silver ions using a sodium borohydride as a reducing agent. Subsequently, hydrogels
were synthesized via photopolymerization and subjected to studies aiming at
characterizing their sorption properties, surface morphology via scanning electron
microscopy, and their impact on simulated physiological liquids supported by
defining these liquids' influence on hydrogels' structures by FT-IR spectroscopy.
Next, the tensile strength of hydrogels and their percentage elongation were
determined. Performed studies also allowed for determining the hydrogels'
wettability and free surface energies. Finally, the cytotoxicity of hydrogels
towards L929 murine fibroblasts via the MTT reduction assay was also verified. It
was demonstrated that developed materials showed stability in simulated
physiological liquids. Moreover, hydrogels were characterized by high elasticity
(percentage elongation within the range of 24-29%), and their surfaces were
hydrophilic (wetting angles below 90 degrees). Hydrogels containing both licorice
extract and nanosilver showed smooth and homogeneous surfaces. Importantly,
cytotoxic properties towards L929 murine fibroblasts were excluded; thus, developed
materials seem to have great potential for application as innovative dressings.
AN - WOS:000909051600001
AU - Kedzierska, M.
AU - Bankosz, M.
AU - Drabczyk, A.
AU - Kudlacik-Kramarczyk, S.
AU - Jamrozy, M.
AU - Potemski, P.
C7 - 217
DA - JAN
DO - 10.3390/ijms24010217
IS - 1
PY - 2023
SN - 1422-0067
ST - Silver Nanoparticles and Glycyrrhiza glabra (Licorice) Root Extract as
Modifying Agents of Hydrogels Designed as Innovative Dressings
TI - Silver Nanoparticles and Glycyrrhiza glabra (Licorice) Root Extract as
Modifying Agents of Hydrogels Designed as Innovative Dressings
VL - 24
ID - 5958
ER -
TY - JOUR
AB - The interest in magnetic nanoparticles is constantly growing, which is due to
their unique properties, of which the most useful is the possibility of directing
their movement via an external magnetic field. Thus, applications may be found for
them as carriers in targeted drug delivery. These nanomaterials usually form a core
in a core–shell structure, and a shell may be formed via various compounds. Here,
nanosilver-shelled iron oxide magnetic nanoparticles were developed. Various
reaction media and various Arabic gum (stabilizer) solution concentrations were
investigated to verify those that were most beneficial one in limiting their
agglomeration as much as possible. The essential oil of lavender was proposed as a
component of such a medium; it was used alone or in combination with distilled
water as a solvent of the stabilizer. The particle size was characterized by
dynamic light scattering (DLS), the chemical structure was characterized via FT-IR
spectroscopy, the crystallinity was characterized by X-ray diffraction (XRD), and
the surface morphology and elemental composition were verified via the SEM-EDS
technique. Moreover, UV-Vis spectrophotometry was used to verify the presence of
the shell made of nanosilver. Importantly, the particles’ pro-inflammatory activity
and cytotoxicity towards L929 murine fibroblasts were also characterized. It was
demonstrated that a 3% stabilizer solution provided a preparation of Fe3O4@Ag
particles, but its stabilizing effect was not sufficient, as a suspension with
micrometric particles was obtained; thus it was necessary to apply 4 h of
sonication for their crushing. Next, the oil/water reaction medium was verified as
beneficial in terms of nanoparticle formation. In such reaction conditions, the
formation of particle agglomerates was strongly limited, and after 15 min of
sonication a suspension containing only nanoparticles was obtained. The presence of
a nanosilver shell was confirmed spectrophotometrically via XRD and SEM-EDS
techniques. Importantly, the developed nanomaterials showed no cytotoxicity towards
murine fibroblasts and no pro-inflammatory activity. © 2022 by the authors.
AU - Kędzierska, M.
AU - Drabczyk, A.
AU - Jamroży, M.
AU - Kudłacik-Kramarczyk, S.
AU - Głąb, M.
AU - Potemski, P.
AU - Tyliszczak, B.
C7 - 4050
DB - Scopus
DO - 10.3390/ma15124050
IS - 12
KW - arabic gum
colloidal stability
core–shell nanostructures
iron oxide magnetic nanoparticles
pro-inflammatory activity
ultrasound-assisted agglomerate crushing
Cell culture
Cell proliferation
Crystallinity
Cytotoxicity
Magnetite
Morphology
Particle size
Sols
Sonication
Surface morphology
Suspensions (fluids)
Ultrasonic applications
X ray diffraction
Arabic gums
Colloidal Stability
Core shell nano structures
Core-shell nanostructures
Inflammatory activity
Iron oxide magnetic nanoparticle
Nano silver
Pro-inflammatory activity
Stabiliser
Ultrasound-assisted agglomerate crushing
M3 - Article
PY - 2022
ST - Iron Oxide Magnetic Nanoparticles with a Shell Made from Nanosilver—Synthesis
Methodology and Characterization of Physicochemical and Biological Properties
T2 - Materials
TI - Iron Oxide Magnetic Nanoparticles with a Shell Made from Nanosilver—Synthesis
Methodology and Characterization of Physicochemical and Biological Properties
85132132518&doi=10.3390%2fma15124050&partnerID=40&md5=ab9a386c5ccc6ec29c4e74b682906
be0
VL - 15
ID - 5087
ER -
TY - JOUR
AB - Chronic non-healing wounds represent a substantial economic burden to
healthcare systems and cause a considerable reduction in quality of life for those
affected. Approximately 0.5–2% of the population in developed countries are
projected to experience a chronic wound in their lifetime, necessitating further
developments in the area of wound care materials. The use of aerogels for wound
healing applications has increased due to their high exudate absorbency and ability
to incorporate therapeutic substances, amongst them trace metals, to promote wound-
healing. This study evaluates the swelling behavior of Ca-Zn-Ag-loaded alginate
aerogels and their metal release upon incubation in human sweat or wound fluid
substitutes. All aerogels show excellent liquid uptake from any of the formulas and
high liquid holding capacities. Calcium is only marginally released into the
swelling solvents, thus remaining as alginate bridging component aiding the
absorption and fast transfer of liquids into the aerogel network. The zinc transfer
quota is similar to those observed for common wound dressings in human and animal
injury models. With respect to the immune regulatory function of zinc, cell culture
studies show a high availability and anti-inflammatory activity of aerogel released
Zn-species in RAW 264.7 macrophages. For silver, the balance between antibacterial
effectiveness versus cytotoxicity remains a significant challenge for which the
alginate aerogels need to be improved in the future. An increased knowledge of the
transformations that alginate aerogels undergo in the course of the fabrication as
well as during wound fluid exposure is necessary when aiming to create advanced,
tissue-compatible aerogel products. © 2020 by the authors. Licensee MDPI, Basel,
Switzerland.
AU - Keil, C.
AU - Hübner, C.
AU - Richter, C.
AU - Lier, S.
AU - Barthel, L.
AU - Meyer, V.
AU - Subrahmanyam, R.
AU - Gurikov, P.
AU - Smirnova, I.
AU - Haase, H.
C7 - 2741
DB - Scopus
DO - 10.3390/polym12112741
IS - 11
KW - Aerogel
Albumin
Alginate
Anti-inflammatory
Antibacterial
Nitric monoxide
Silver
Toll-like receptor
Wound dressing
Zinc
Behavioral research
Body fluids
Calcium alloys
Cell culture
Liquids
Macrophages
Silver alloys
Swelling
Ternary alloys
Tissue regeneration
Trace elements
Zinc alloys
Alginate aerogels
Developed countries
Further development
Health-care system
Immune regulatory functions
Raw 264.7 macrophages
Aerogels
M3 - Article
PY - 2020
SP - 1-17
ST - Ca-Zn-Ag alginate aerogels for wound healing applications: Swelling behavior
in simulated human body fluids and effect on macrophages
T2 - Polymers
TI - Ca-Zn-Ag alginate aerogels for wound healing applications: Swelling behavior
in simulated human body fluids and effect on macrophages
85096596519&doi=10.3390%2fpolym12112741&partnerID=40&md5=fad72d0476fae276854226367f
31839b
VL - 12
ID - 5294
ER -
TY - JOUR
AB - Chronic non-healing wounds represent a substantial economic burden to
healthcare systems and cause a considerable reduction in quality of life for those
affected. Approximately 0.5-2% of the population in developed countries are
projected to experience a chronic wound in their lifetime, necessitating further
developments in the area of wound care materials. The use of aerogels for wound
healing applications has increased due to their high exudate absorbency and ability
to incorporate therapeutic substances, amongst them trace metals, to promote wound-
healing. This study evaluates the swelling behavior of Ca-Zn-Ag-loaded alginate
aerogels and their metal release upon incubation in human sweat or wound fluid
substitutes. All aerogels show excellent liquid uptake from any of the formulas and
high liquid holding capacities. Calcium is only marginally released into the
swelling solvents, thus remaining as alginate bridging component aiding the
absorption and fast transfer of liquids into the aerogel network. The zinc transfer
quota is similar to those observed for common wound dressings in human and animal
injury models. With respect to the immune regulatory function of zinc, cell culture
studies show a high availability and anti-inflammatory activity of aerogel released
Zn-species in RAW 264.7 macrophages. For silver, the balance between antibacterial
effectiveness versus cytotoxicity remains a significant challenge for which the
alginate aerogels need to be improved in the future. An increased knowledge of the
transformations that alginate aerogels undergo in the course of the fabrication as
well as during wound fluid exposure is necessary when aiming to create advanced,
tissue-compatible aerogel products.
AN - WOS:000593785300001
AU - Keil, C.
AU - Hubner, C.
AU - Richter, C.
AU - Lier, S.
AU - Barthel, L.
AU - Meyer, V.
AU - Subrahmanyam, R.
AU - Gurikov, P.
AU - Smirnova, I.
AU - Haase, H.
C7 - 2741
DA - NOV
DO - 10.3390/polym12112741
IS - 11
PY - 2020
SN - 2073-4360
ST - Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior
in Simulated Human Body Fluids and Effect on Macrophages
T2 - POLYMERS
TI - Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior
in Simulated Human Body Fluids and Effect on Macrophages
VL - 12
ID - 6265
ER -
TY - JOUR
AB - Engineered nanoparticles are increasingly used in medical applications and
day-to-day consumer products, leading to concerns about the potential environmental
and human health impacts. Silver nanoparticles are particularly prevalent because
of their use as antibacterial agents in many commonly available products.
Nanoparticles (NPs) are believed to accumulate, often preferentially, in the liver.
This study therefore investigates the effect of a silver NP (20 nm) on the liver,
and in particular, the role of Kupffer cells (KCs; resident liver macrophages) in
the overall inflammatory response in the organ. Cytokine expression in the normal
liver was measured in terms of IL2, IL4, TNF-alpha, IFN-gamma and IL10 released
from the organ with significant up-regulation of TNF-alpha and IL10 being observed.
For livers in which the KC population was specifically targeted and destroyed this
cytokine increase was significantly decreased in comparison to the normal tissue.
IL10 was secreted at approximately three times the concentration of TNF-alpha in
all the test cases. The high levels of IL10 released from the normal tissue in
comparison to the KC depleted livers suggest that the cytokine may help to protect
against a pro-inflammatory response to these Ag NPs. This may indicate a
potentially important role for KCs in the anti-inflammatory response and suggests
that tolerance to the Ag NPs is favoured over a fully activated immune response. In
addition, albumin production was measured as an indicator of hepatic function. It
was noted that the liver function was unaffected by the Ag NPs.
AN - WOS:000340524300015
AU - Chauche, C.
AU - Balharry, D.
AU - Brown, D. M.
AU - Kanase, N.
AU - Boczkowski, J.
AU - Lanone, S.
AU - Stone, V.
DA - AUG
DO - 10.3109/17435390.2013.866284
PY - 2014
SN - 1743-5390
1743-5404
SP - 149-154
ST - The role of Kupffer cells in the hepatic response to silver nanoparticles
T2 - NANOTOXICOLOGY
TI - The role of Kupffer cells in the hepatic response to silver nanoparticles
VL - 8
ID - 5938
ER -
TY - JOUR
AB - The use of hepatocyte cell lines as a replacement for animal models have been
heavily criticised mainly due to low expression of metabolism enzymes. This study
compares primary human hepatocytes with the C3A cell line and with respect to their
response to a panel of nanomaterials (NMs; two ZnO, two MWCNTs, one Ag and one
positively functionalised TiO2). The cell line was very comparable with the primary
hepatocytes with regards to their cytotoxic response to the NMs (Ag > uncoated ZnO
> coated ZnO). The LC50 was not attained in the presence of the MWCNTs and the TiO2
NMs. All NMs significantly increased IL-8 production, with no change in levels of
TNF-α and IL-6. Albumin production was measured as an indicator of hepatic
function. The authors found no change in levels of albumin with the exception of
the coated ZnO NM at the LC50 concentration. NM uptake was similar for both the
primary hepatocytes and C3A cells as investigated by TEM. Meanwhile, the authors
confirmed greater levels of CYP450 activity in untreated primary cells. This study
demonstrates that the C3A cell line is a good model for investigating NM-induced
hepatocyte responses with respect to uptake, cytotoxicity, pro-inflammatory
cytokine production and albumin production. © Informa UK, Ltd.
AU - Gaiser, B. K.
AU - Ward, M. B.
AU - Stone, V.
DB - Scopus
DO - 10.3109/17435390.2012.734341
IS - 7
KW - Cytokine production
Cytotoxicity
Liver
Nanomaterial uptake
Albumins
Animal Testing Alternatives
Cell Line, Tumor
Cell Survival
Cytokines
Endocytosis
Hepatocytes
Humans
Lethal Dose 50
Nanoparticles
Particle Size
Surface Properties
Toxicity Tests
albumin
interleukin 6
interleukin 8
nanomaterial
silver
titanium dioxide
zinc oxide
article
cancer cell culture
cell viability
controlled study
cytokine production
cytotoxicity
human
human cell
in vitro study
LC 50
liver cell
liver function
nanotoxicology
priority journal
M3 - Article
PY - 2013
SP - 1255-1271
ST - Primary human hepatocytes versus hepatic cell line: Assessing their
suitability for in vitro nanotoxicology
T2 - Nanotoxicology
TI - Primary human hepatocytes versus hepatic cell line: Assessing their
suitability for in vitro nanotoxicology
84885145308&doi=10.3109%2f17435390.2012.734341&partnerID=40&md5=fa21a15c424fa31bfde
87d6a6c28b5f4
VL - 7
ID - 5618
ER -
TY - JOUR
AB - Effects on the liver C3A cell line treated with a panel of engineered
nanomaterials (NMs) consisting of two zinc oxide particles (ZnO; coated 100 nm and
uncoated 130 nm), two multi-walled carbon nanotubes (MWCNTs), one silver (Ag < 20
nm), one 7 nm anatase, two rutile TiO2 nanoparticles (10 and 94 nm) and two
derivatives with positive and negative covalent functionalisation of the 10 nm
rutile were evaluated. The silver particles elicited the greatest level of
cytotoxicity (24 h LC50-2 μg/cm2). The silver was followed by the uncoated ZnO (24
h LC50-7.5 μg/cm2) and coated ZnO (24 h LC50-15 μg/cm2) particles with respect to
cytotoxicity. The ZnO NMs were found to be about 50-60% soluble which could account
for their toxicity. By contrast, the Ag was <1% soluble. The LC50 was not attained
in the presence of any of the other engineered NMs (up to 80 μg/cm2). All NMs
significantly increased IL-8 production. Meanwhile, no significant change in TNF-α,
IL-6 or CRP was detected. Urea and albumin production were measured as indicators
of hepatic function. These markers were only altered by the coated and uncoated
ZnO, which significantly decreased albumin production. © 2013 Informa UK, Ltd.
AU - Pojana, G.
AU - Gaiser, B. K.
AU - Birkedal, R.
AU - Bilaničová, D.
AU - Wallin, H.
AU - Jensen, K. A.
AU - Sellergren, B.
AU - Marcomini, A.
AU - Stone, V.
DB - Scopus
DO - 10.3109/17435390.2011.653416
IS - 3
KW - Albumin
IL-8
Inflammation
Liver
Urea
Albumins
Biological Markers
Cell Line, Tumor
Cell Survival
Cytokines
Hepatocytes
Humans
Nanostructures
Silver
Titanium
Zinc Oxide
albumin
biological marker
C reactive protein
interleukin 6
interleukin 8
nanomaterial
silver nanoparticle
titanium dioxide
urea
zinc oxide
article
cell viability
controlled study
cytokine production
cytokine release
cytopathogenic effect
human
human cell
in vitro study
LC 50
liver cell
liver function test
material coating
nanoengineering
priority journal
protein synthesis
solubility
M3 - Article
PY - 2013
SP - 301-313
ST - In vitro assessment of engineered nanomaterials using a hepatocyte cell line:
Cytotoxicity, pro-inflammatory cytokines and functional markers
T2 - Nanotoxicology
TI - In vitro assessment of engineered nanomaterials using a hepatocyte cell line:
Cytotoxicity, pro-inflammatory cytokines and functional markers
84876037765&doi=10.3109%2f17435390.2011.653416&partnerID=40&md5=b0ad60bb40d50f6f974
026de9eac8d06
VL - 7
ID - 5658
ER -
TY - JOUR
AB - Effects on the liver C3A cell line treated with a panel of engineered
nanomaterials (NMs) consisting of two zinc oxide particles (ZnO; coated 100 nm and
uncoated 130 nm), two multiwalled carbon nanotubes (MWCNTs), one silver (Ag < 20
nm), one 7 nm anatase, two rutile TiO2 nanoparticles (10 and 94 nm) and two
derivatives with positive and negative covalent functionalisation of the 10 nm
rutile were evaluated. The silver particles elicited the greatest level of
cytotoxicity (24 h LC50 - 2 mu g/cm(2)). The silver was followed by the uncoated
ZnO (24 h LC50 - 7.5 mu g/cm(2)) and coated ZnO (24 h LC50 - 15 mu g/cm(2))
particles with respect to cytotoxicity. The ZnO NMs were found to be about 50-60%
soluble which could account for their toxicity. By contrast, the Ag was <1%
soluble. The LC50 was not attained in the presence of any of the other engineered
NMs (up to 80 mu g/cm(2)). All NMs significantly increased IL-8 production.
Meanwhile, no significant change in TNF-alpha, IL-6 or CRP was detected. Urea and
albumin production were measured as indicators of hepatic function. These markers
were only altered by the coated and uncoated ZnO, which significantly decreased
albumin production.
AN - WOS:000317497000006
AU - Pojana, G.
AU - Gaiser, B. K.
AU - Birkedal, R.
AU - Bilanicova, D.
AU - Wallin, H.
AU - Jensen, K. A.
AU - Sellergren, B.
AU - Marcomini, A.
AU - Stone, V.
DA - MAY
DO - 10.3109/17435390.2011.653416
IS - 3
PY - 2013
SN - 1743-5390
1743-5404
SP - 301-313
ST - In vitro assessment of engineered nanomaterials using a hepatocyte cell line:
cytotoxicity, pro-inflammatory cytokines and functional markers
T2 - NANOTOXICOLOGY
TI - In vitro assessment of engineered nanomaterials using a hepatocyte cell line:
cytotoxicity, pro-inflammatory cytokines and functional markers
VL - 7
ID - 6206
ER -
TY - JOUR
AB - The liver is one of the most important multi-functional organs in the human
body. Amongst various crucial functions, it is the main detoxification center and
predominantly implicated in the clearance of xenobiotics potentially including
particulates that reach this organ. It is now well established that a significant
quantity of injected, ingested or inhaled nanomaterials (NMs) translocate from
primary exposure sites and accumulate in liver. This review aimed to summarize and
discuss the progress made in the field of hepatic nanotoxicology, and crucially
highlight knowledge gaps that still exist. Key considerations include In vivo
studies clearly demonstrate that low-solubility NMs predominantly accumulate in the
liver macrophages the Kupffer cells (KC), rather than hepatocytes. KCs lining the
liver sinusoids are the first cell type that comes in contact with NMs in vivo.
Further, these macrophages govern overall inflammatory responses in a healthy
liver. Therefore, interaction with of NM with KCs in vitro appears to be very
important. Many acute in vivo studies demonstrated signs of toxicity induced by a
variety of NMs. However, acute studies may not be that meaningful due to liver's
unique and unparalleled ability to regenerate. In almost all investigations where a
recovery period was included, the healthy liver was able to recover from NM
challenge. This organ's ability to regenerate cannot be reproduced in vitro.
However, recommendations and evidence is offered for the design of more
physiologically relevant in vitro models. Models of hepatic disease enhance the NM-
induced hepatotoxicity. The review offers a number of important suggestions for the
future of hepatic nanotoxicology study design. This is of great significance as its
findings are highly relevant due to the development of more advanced in vitro, and
in silico models aiming to improve physiologically relevant toxicological testing
strategies and bridging the gap between in vitro and in vivo experimentation.
AN - WOS:000528378400001
AU - Powell, L. G.
AU - Stone, V.
C6 - APR 2020
DA - MAY 18
DO - 10.1080/10937404.2020.1751756
IS - 4
PY - 2020
SN - 1093-7404
1521-6950
SP - 137-176
ST - A review of hepatic nanotoxicology - summation of recent findings and
considerations for the next generation of study designs
T2 - JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART B-CRITICAL REVIEWS
TI - A review of hepatic nanotoxicology - summation of recent findings and
considerations for the next generation of study designs
VL - 23
ID - 6713
ER -
TY - JOUR
AB - Background: It has been shown that nanomaterials (NMs) are able to
translocate to secondary tissues one of the important being the kidneys. Oxidative
stress has been implicated as a possible mechanism for NM toxicity, hence effects
on the human renal proximal tubule epithelial cells (HK-2) treated with a panel of
engineered nanomaterials (NMs) consisting of two zinc oxide particles (ZnO - coated
- NM 110 and uncoated - NM 111), two multi walled carbon nanotubes (MWCNT) (NM 400
and NM 402), one silver (NM 300) and five TiO 2 NMs (NM 101, NRCWE 001, 002, 003
and 004) were evaluated. Methods. In order to assess the toxicological impact of
the engineered NMs on HK-2 cells - WST-1 cytotoxicity assay, FACSArray, HE
oxidation and the comet assays were utilised. For statistical analysis, the
experimental values were compared to their corresponding controls using an ANOVA
with Tukey's multiple comparison. Results: We found the two ZnO NMs (24 hr LC50 -
2.5 μg/cm 2) and silver NM (24 hr LC50 - 10 μg/cm2) were highly cytotoxic to the
cells. The LC50 was not attained in the presence of any of the other engineered
nanomaterials (up to 80 μg/cm 2). All nanomaterials significantly increased IL8 and
IL6 production. Meanwhile no significant change in TNF- or MCP-1 was detectable.
The most notable increase in ROS was noted following treatment with the Ag and the
two ZnO NMs. Finally, genotoxicity was measured at sub-lethal concentrations. We
found a small but significant increase in DNA damage following exposure to seven of
the ten NMs investigated (NM 111, NRCWE 001 and NRCWE 003 being the exception) with
this increase being most visible following exposure to Ag and the positively
charged TiO2. Conclusions: While the NMs could be categorised as low and highly
cytotoxic, sub-lethal effects such as cytokine production and genotoxicity were
observed with some of the low toxicity materials. © 2013 Kermanizadeh et al.;
licensee BioMed Central Ltd.
AU - Vranic, S.
AU - Boland, S.
AU - Moreau, K.
AU - Gaiser, B. K.
AU - Andrzejczuk, L. A.
AU - Stone, V.
C7 - 96
DB - Scopus
DO - 10.1186/1471-2369-14-96
IS - 1
KW - DNA damage
Inflammation
Kidneys
Nanotoxicity
ROS
Animals
Biomedical Engineering
Cattle
Cell Line
Cell Line, Transformed
Cytotoxins
DNA Damage
Humans
Kidney
Nanostructures
Oxidative Stress
interleukin 6
interleukin 8
nanomaterial
silver nanoparticle
unclassified drug
article
cell viability
clinical assessment
comet assay
cytokine production
cytokine release
drug cytotoxicity
genotoxicity
human
human cell
human tissue
in vitro study
kidney tubule cell
LD 50
nanoengineering
oxidative stress
M3 - Article
PY - 2013
ST - An in vitro assessment of panel of engineered nanomaterials using a human
renal cell line: Cytotoxicity, pro-inflammatory response, oxidative stress and
genotoxicity
T2 - BMC Nephrology
TI - An in vitro assessment of panel of engineered nanomaterials using a human
renal cell line: Cytotoxicity, pro-inflammatory response, oxidative stress and
genotoxicity
84876999447&doi=10.1186%2f1471-2369-14-
96&partnerID=40&md5=d90d7a928460d49092be2bfd50d15903
VL - 14
ID - 5694
ER -
TY - JOUR
AB - The purpose of this review is to examine the parallels between the effects
mercury intoxication on the brain and the brain pathology found in autism spectrum
disorder (ASD). This review finds evidence of many parallels between the two,
including: (1) microtubule degeneration, specifically large, long-range axon
degeneration with subsequent abortive axonal sprouting (short, thin axons); (2)
dentritic overgrowth; (3) neuroinflammation; (4) microglial/astrocytic activation;
(5) brain immune response activation; (6) elevated glial fibrillary acidic protein;
(7) oxidative stress and lipid peroxidation; (8) decreased reduced glutathione
levels and elevated oxidized glutathione; (9) mitochondrial dysfunction; (10)
disruption in calcium homeostasis and signaling; (11) inhibition of glutamic acid
decarboxylase (GAD) activity; (12) disruption of GABAergic and glutamatergic
homeostasis; (13) inhibition of IGF-1 and methionine synthase activity; (14)
impairment in methylation; (15) vascular endothelial cell dysfunction and
pathological changes of the blood vessels; (16) decreased cerebral/cerebellar blood
flow; (17) increased amyloid precursor protein; (18) loss of granule and Purkinje
neurons in the cerebellum; (19) increased pro-inflammatory cytokine levels in the
brain (TNF-alpha, IFN-gamma, IL-1 beta, IL-8); and (20) aberrant nuclear factor
kappa-light-chain-enhancer of activated B cells (NF-kappa B). This review also
discusses the ability of mercury to potentiate and work synergistically with other
toxins and pathogens in a way that may contribute to the brain pathology in ASD.
The evidence suggests that mercury may be either causal or contributory in the
brain pathology in ASD, possibly working synergistically with other toxic compounds
or pathogens to produce the brain pathology observed in those diagnosed with an
ASD.
AN - WOS:000306631200001
AU - Kern, J. K.
AU - Geier, D. A.
AU - Audhya, T.
AU - King, P. G.
AU - Sykes, L. K.
AU - Geier, M. R.
IS - 2
PY - 2012
SN - 0065-1400
1689-0035
SP - 113-153
ST - Evidence of parallels between mercury intoxication and the brain pathology in
autism
T2 - ACTA NEUROBIOLOGIAE EXPERIMENTALIS
TI - Evidence of parallels between mercury intoxication and the brain pathology in
autism
VL - 72
ID - 6758
ER -
TY - JOUR
AB - Mustard gas (sulfur mustard [SM], bis-[2-chloroethyl] sulfide) is a
vesicating chemical warfare agent and a potential chemical terrorism agent.
Exposure of SM causes debilitating skin blisters (vesication) and injury to the
eyes and the respiratory tract; of these, the respiratory injury, if severe, may
even be fatal. Therefore, developing an effective therapeutic strategy to protect
against SM-induced respiratory injury is an urgent priority of not only the US
military but also the civilian antiterrorism agencies, for example, the Homeland
Security. Toward developing a respiratory medical countermeasure for SM, four
different classes of therapeutic compounds have been evaluated in the past: anti-
inflammatory compounds, antioxidants, protease inhibitors and antiapoptotic
compounds. This review examines all of these different options; however, it
suggests that preventing cell death by inhibiting apoptosis seems to be a
compelling strategy but possibly dependent on adjunct therapies using the other
drugs, that is, anti-inflammatory, antioxidant, and protease inhibitor compounds.
AN - WOS:000340545500003
AU - Keyser, B. M.
AU - Andres, D. K.
AU - Holmes, W. W.
AU - Paradiso, D.
AU - Appell, A.
AU - Letukas, V. A.
AU - Benton, B.
AU - Clark, O. E.
AU - Gao, X. G.
AU - Ray, P.
AU - Anderson, D. R.
AU - Ray, R.
DA - JUL-AUG
DO - 10.1177/1091581814532959
IS - 4
PY - 2014
SN - 1091-5818
1092-874X
SP - 271-281
ST - Mustard Gas Inhalation Injury: Therapeutic Strategy
T2 - INTERNATIONAL JOURNAL OF TOXICOLOGY
TI - Mustard Gas Inhalation Injury: Therapeutic Strategy
VL - 33
ID - 6225
ER -
TY - JOUR
AB - Healing of equine distal limb wound represents a challenging problem and
occupies a vital place in everyday pathology. Silver nanoparticles (AgNPs) are
attractive materials for many researchers because of their anti-inflammatory,
antibacterial, and wound-healing properties; as such, this study was performed to
investigate the healing efficacy of AgNPs on second-intention wound healing in five
clinically healthy donkeys as compared with silver sulfadiazine. Wound healing was
evaluated clinically (healing rate [%], healing time [days], epithelialization rate
[%]), whereas the antimicrobial effect of AgNPs was evaluated by comparing with
amoxicillin/metronidazole mixture. In addition, angiogenesis, re-epithelialization,
cellular invasion, and scar width were characterized by histopathology and the
immunohistochemical localization of transforming growth factor beta 1 (TGF beta 1)
was assessed, along with the ultrastructure of the AgNPs-treated wounds.
Accelerated wound healing, better cosmetic appearance, and efficient antimicrobial
effect were achieved with AgNPs dressing. Superiority of AgNPs in wound healing was
approved histopathologically by the early granulation tissue formation, higher
epithelialization (%), and reduced scar width. Positive TGF beta 1 immunoreactivity
with macrophage, fibroblast, and endothelial localization was reported during the
inflammatory and the proliferative phase; however, it has reduced during the
remolding phase producing a faster scar-free wound healing. The cytocompatibility
of the AgNPs with fibroblast was demonstrated by the integrity of the cellular and
nuclear membrane with nonfragmented nucleoli, as detected by the ultrastructural
examination. It could be concluded that AgNPs represent a step forward and a
promising alternative dressing in wound healing in donkeys through its anti-
inflammatory, antimicrobial, and favorable cosmetic properties, in addition to its
role in regulation of TGF beta 1 production. (c) 2017 Elsevier Inc. All rights
reserved.
AN - WOS:000425880400012
AU - Khafaga, A. F.
AU - Abu-Ahmed, H. M.
AU - El-Khamary, A. N.
AU - Elmehasseb, I. M.
AU - Shaheen, H. M.
DA - FEB
DO - 10.1016/j.jevs.2017.11.013
PY - 2018
SN - 0737-0806
1542-7412
SP - 76-87
ST - Enhancement of Equid Distal Limb Wounds Healing by Topical Application of
T2 - JOURNAL OF EQUINE VETERINARY SCIENCE
TI - Enhancement of Equid Distal Limb Wounds Healing by Topical Application of
VL - 61
ID - 6030
ER -
TY - JOUR
AB - Biosynthesized silver nanoparticles (Bio-SNPs) were synthesized from the
marine actinobacterium strain Streptomyces catenulae M2 and characterized using a
variety of techniques, including UV–vis spectrum, fourier transform infrared
spectroscopy (FTIR), energy dispersive x-ray (EDX), transmission electron
microscopy (TEM), dynamic light scattering (DLS), surface-enhanced Raman
spectroscopy (SERS), and zeta potential. The antibacterial activity of Bio-SNPs
alone and in combination with antibiotic was evaluated using a microtiter-dilution
resazurin assay against multidrug-resistant (MDR) bacteria. Bio-SNPs’ minimum
inhibitory concentration (MIC) against bacterial strains was determined. To assess
the synergistic effect of Bio-SNPs in combination with antibiotics, the Fractional
Inhibitory Concentration Index (FICI) was calculated. While the safety of Bio-SNPs
in biomedical applications is dependent on their use, the in vitro cytotoxicity of
Bio-SNPs on normal human epithelial colon cells (NCM460) and human colorectal
adenocarcinoma cells (CaCo2) were evaluated using the [3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyltetrazolium bromide] (MTT) assay and cell lactate dehydrogenase
(LDH) release. The presence of Bio-SNPs was revealed by UV–vis spectroscopy, which
revealed a peak in the Surface Plasmon Resonance (SPR) spectrum at 439.5 nm. Bio-
SNPs were spherical in shape and small in size (average 33 nm by TEM, 58.8 nm by
DLS), with good stability (−30 mV) and the presence of capping agents. Bio-SNPs had
MIC values ranging from 2 to 64 μg/ml against the bacteria tested. The MIC for P.
aeruginosa was the lowest (2 μg/ml). Antibiotics have been shown to have a
significant synergistic effect when combined with Bio-SNPs against tested bacteria.
Bio-SNPs exhibited dose-dependent cytotoxicity against NCM460 and CaCo2 cancer
cells, with the latter exhibiting far greater toxicity than the former. NCM460
and CaCo2 cell viability decreased from 99.3 to 95.7% and 92.3 to 61.8%,
respectively, whereas LDH leakage increased from 200 to 215 nmol/ml and 261 to 730
nmol/ml, respectively. The half inhibitory concentrations (IC50) for NCM460 and
CaCo2 cancer cells were 79.46 and 10.41 μg/ml and 89.4 and 19.3 μg/ml,
respectively. Bio-SNPs were found to be biocompatible and to have anti-inflammatory
activity. Bio-SNPs are highly appealing for future nanomedicine applications due to
their antibacterial and biocompatible properties and their inherent “green” and
simple manufacturing. Copyright © 2022 Khalil, El-Shanshoury, Alghamdi, Sun and
Ali.
AU - Khalil, M. A.
AU - El-Shanshoury, A. E. R. R.
AU - Alghamdi, M. A.
AU - Sun, J.
AU - Ali, S. S.
C7 - 833154
DB - Scopus
DO - 10.3389/fmicb.2022.833154
antibacterial
antioxidant
antitumor
marine actinobacteria
antibiotic agent
silver nanoparticle
streptomycin
Actinobacteria
Article
biological activity
biosynthesis
cancer cell
cell culture
cell viability
controlled study
cytotoxicity
epithelium cell
Escherichia coli
fetal bovine serum
MTT assay
nonhuman
Raman spectrometry
Streptomyces
synergistic effect
M3 - Article
PY - 2022
ST - Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver
Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of
Antibacterial Action
TI - Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver
85130150645&doi=10.3389%2ffmicb.2022.833154&partnerID=40&md5=1bc4ec7320075a5655eea6
10cea5bd25
VL - 13
ID - 5107
ER -
TY - JOUR
AB - Biosynthesized silver nanoparticles (Bio-SNPs) were synthesized from the
marine actinobacterium strain Streptomyces catenulae M2 and characterized using a
variety of techniques, including UV-vis spectrum, fourier transform infrared
spectroscopy (FTIR), energy dispersive x-ray (EDX), transmission electron
microscopy (TEM), dynamic light scattering (DLS), surface-enhanced Raman
spectroscopy (SERS), and zeta potential. The antibacterial activity of Bio-SNPs
alone and in combination with antibiotic was evaluated using a microtiter-dilution
resazurin assay against multidrug-resistant (MDR) bacteria. Bio-SNPs' minimum
inhibitory concentration (MIC) against bacterial strains was determined. To assess
the synergistic effect of Bio-SNPs in combination with antibiotics, the Fractional
Inhibitory Concentration Index (FICI) was calculated. While the safety of Bio-SNPs
in biomedical applications is dependent on their use, the in vitro cytotoxicity of
Bio-SNPs on normal human epithelial colon cells (NCM460) and human colorectal
adenocarcinoma cells (CaCo2) were evaluated using the [3(4,5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] (MTT) assay and cell lactate dehydrogenase (LDH)
release. The presence of Bio-SNPs was revealed by UV-vis spectroscopy, which
revealed a peak in the Surface Plasmon Resonance (SPR) spectrum at 439.5 nm. Bio-
SNPs were spherical in shape and small in size (average 33 nm by TEM, 58.8 nm by
DLS), with good stability (30 mV) and the presence of capping agents. Bio-SNPs had
MIC values ranging from 2 to 64 mu g/ml against the bacteria tested. The MIC for P.
aeruginosa was the lowest (2 mu g/ml). Antibiotics have been shown to have a
significant synergistic effect when combined with BioSNPs against tested bacteria.
Bio-SNPs exhibited dose-dependent cytotoxicity against NCM460 and CaCo2 cancer
cells, with the latter exhibiting far greater toxicity than the former. NCM460 and
CaCo2 cell viability decreased from 99.3 to 95.7% and 92.3 to 61.8%, respectively,
whereas LDH leakage increased from 200 to 215 nmol/ml and 261 to 730 nmol/ml,
respectively. The half inhibitory concentrations (IC50) for NCM460 and CaCo2 cancer
cells were 79.46 and 10.41 mg/ml and 89.4 and 19.3 mg/ml, respectively. Bio-SNPs
were found to be biocompatible and to have anti-inflammatory activity. Bio-SNPs are
highly appealing for future nanomedicine applications due to their antibacterial
and biocompatible properties and their inherent "green" and simple manufacturing.
AN - WOS:000795674800001
AU - Khalil, M. A.
AU - El-Shanshoury, A. R.
AU - Alghamdi, M. A.
AU - Sun, J. Z.
AU - Ali, S. S.
C7 - 833154
DA - APR 28
DO - 10.3389/fmicb.2022.833154
PY - 2022
SN - 1664-302X
ST - Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver
T2 - FRONTIERS IN MICROBIOLOGY
TI - Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver
VL - 13
ID - 5909
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been reported to penetrate the central
nervous system (CNS) and induce neurotoxicity. However, there is a paucity of
understanding of the toxicity of AgNPs and their effect on the blood-brain barrier
(BBB) including the underlying molecular mechanism(s) of action. Such information
is important for the formulation of new strategies for delivery of biological
therapeutics to central nervous system (CNS) targets. Using an in vitro BBB model
and mass spectrometry-based proteomics, we investigated alterations in the
proteomes of brain endothelial cells and astrocytes at different time points after
AgNPs exposure (24 and 48h). Our data showed that several proteins involved in
neurodisorders and neurodegeneration were significantly upregulated in endothelial
cells (e.g. 7-dehydrocholesterol reductase, zinc transporters 1 and 6), while
proteins responsible for maintaining brain homeostasis were significantly
downregulated (e.g anti-oxidative proteins glutathione peroxidase 1 and glutathione
peroxidase 4). Many inflammatory pathways were significantly upregulated at 24h
post-AgNPs exposure (C9 pathway), while at 48h proteins involved in BBB damage and
anti-inflammatory responses were upregulated (quinoneoxidoreductase1 and glutamate
cysteine ligase catalytic subunit) suggesting that by the later time point,
cellular protection pathways had been activated to rescue the cells from AgNPs-
induced toxicity. Our study suggests that in the initial stage of exposure, AgNPs
exerted direct cellular stress on the endothelial cells by triggering a pro-
inflammatory cascade. This study provides detailed insight into the toxic potency
of AgNPs on in vitro BBB model and adds to the understanding of the adaptive role
of BBB with regards to AgNPs-mediated toxicity.
AN - WOS:000469102800001
AU - Khan, A. M.
AU - Korzeniowska, B.
AU - Gorshkov, V.
AU - Tahir, M.
AU - Schroder, H.
AU - Skytte, L.
AU - Rasmussen, K. L.
AU - Khandige, S.
AU - Moller-Jensen, J.
AU - Kjeldsen, F.
DA - FEB 7
DO - 10.1080/17435390.2018.1540728
IS - 2
PY - 2019
SN - 1743-5390
1743-5404
SP - 221-239
ST - Silver nanoparticle-induced expression of proteins related to oxidative
stress and neurodegeneration in an in vitro human blood-brain barrier model
T2 - NANOTOXICOLOGY
TI - Silver nanoparticle-induced expression of proteins related to oxidative
VL - 13
ID - 6070
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been reported to penetrate the central
nervous system (CNS) and induce neurotoxicity. However, there is a paucity of
understanding of the toxicity of AgNPs and their effect on the blood-brain barrier
(BBB) including the underlying molecular mechanism(s) of action. Such information
is important for the formulation of new strategies for delivery of biological
therapeutics to central nervous system (CNS) targets. Using an in vitro BBB model
and mass spectrometry-based proteomics, we investigated alterations in the
proteomes of brain endothelial cells and astrocytes at different time points after
AgNPs exposure (24 and 48 h). Our data showed that several proteins involved in
neurodisorders and neurodegeneration were significantly upregulated in endothelial
cells (e.g. 7-dehydrocholesterol reductase, zinc transporters 1 and 6), while
proteins responsible for maintaining brain homeostasis were significantly
downregulated (e.g anti-oxidative proteins glutathione peroxidase 1 and glutathione
peroxidase 4). Many inflammatory pathways were significantly upregulated at 24 h
post-AgNPs exposure (C9 pathway), while at 48 h proteins involved in BBB damage and
anti-inflammatory responses were upregulated (quinoneoxidoreductase1 and glutamate
cysteine ligase catalytic subunit) suggesting that by the later time point,
cellular protection pathways had been activated to rescue the cells from AgNPs-
induced toxicity. Our study suggests that in the initial stage of exposure, AgNPs
exerted direct cellular stress on the endothelial cells by triggering a pro-
inflammatory cascade. This study provides detailed insight into the toxic potency
of AgNPs on in vitro BBB model and adds to the understanding of the adaptive role
of BBB with regards to AgNPs-mediated toxicity. © 2019, © 2019 The Author(s).
Published by Informa UK Limited, trading as Taylor & Francis Group.
AU - Khan, A. M.
AU - Korzeniowska, B.
AU - Gorshkov, V.
AU - Tahir, M.
AU - Schrøder, H.
AU - Skytte, L.
AU - Rasmussen, K. L.
AU - Khandige, S.
AU - Møller-Jensen, J.
AU - Kjeldsen, F.
DB - Scopus
DO - 10.1080/17435390.2018.1540728
IS - 2
KW - Blood brain barrier
mass spectrometry
toxicity
Astrocytes
Blood-Brain Barrier
Cell Survival
Endothelial Cells
Humans
Metal Nanoparticles
Models, Biological
Oxidative Stress
Proteome
Silver
Surface Properties
7 dehydrocholesterol reductase
claudin 5
glutamate cysteine ligase
glutathione peroxidase 1
oxidoreductase
proteome
quinoneoxidoreductase 1
silver nanoparticle
unclassified drug
zinc transporter
zinc transporter 1
zinc transporter 6
metal nanoparticle
silver
Article
astrocyte
blood brain barrier
cell junction
cell migration
cell stress
cell viability
controlled study
down regulation
enzyme induction
exposure
hCMEC/D3 cell line
human
human cell
human tissue
in vitro study
nanotoxicology
nerve degeneration
neurotoxicity
oxidative stress
priority journal
protein expression
proteomics
upregulation
zeta potential
biological model
cell survival
drug effect
endothelium cell
metabolism
pathology
surface property
transport at the cellular level
M3 - Article
PY - 2019
SP - 221-239
ST - Silver nanoparticle-induced expression of proteins related to oxidative
T2 - Nanotoxicology
TI - Silver nanoparticle-induced expression of proteins related to oxidative
85059885536&doi=10.1080%2f17435390.2018.1540728&partnerID=40&md5=6dddca66fa3883da49
eaf4f2bcdeb76b
VL - 13
ID - 5370
ER -
TY - JOUR
AB - Most studies have used in vitro systems to test inflammatory responses of
nanoparticles; these may not reflect the real biological response of body organs.
In fact, certain nanoparticles have provoked opposite effects under in vitro and in
vivo conditions. Current understanding of the biocompatibility of gold
nanoparticles is controversial. We studied the acute (1 day) and sub-chronic (5
days) effects of gold nanoparticles (10 and 50 nm in diameter) on expression of
interleukin-1 beta (IL-1 beta), IL-6 and tumor necrosis factor alpha (TNF-alpha) in
rat liver. Real-time PCR analysis showed that gold nanoparticles of both sizes
significantly increased cytokine gene expression on day 1; this had subsided by day
5. The 50-nm gold nanoparticle produced more severe inflammation than the smaller
gold nanoparticle. These findings indicate a possible biocompatibility of medium-
sized gold nanoparticles, as they caused only a transient increase in
proinflammatory cytokines, followed by normalization during sub-chronic repeated
exposure.
AN - WOS:000331608000172
AU - Khan, H. A.
AU - Abdelhalim, M. A. K.
AU - Alhomida, A. S.
AU - Al Ayed, M. S.
DO - 10.4238/2013.November.22.12
IS - 4
PY - 2013
SN - 1676-5680
SP - 5851-5857
ST - Transient increase in IL-1 beta, IL-6 and TNF-alpha gene expression in rat
liver exposed to gold nanoparticles
T2 - GENETICS AND MOLECULAR RESEARCH
TI - Transient increase in IL-1 beta, IL-6 and TNF-alpha gene expression in rat
liver exposed to gold nanoparticles
VL - 12
ID - 6534
ER -
TY - JOUR
AB - Aquatic contamination with silver nanoparticles (Ag-NPs) can cause toxicity
in target organisms that are directly exposed to them. Oxidative stress and tissue
alterations are potential endpoints of Ag-NPs toxicity. We determined whether
garlic oil can ameliorate the effects of toxicity induced by amine-coated and
spherical Ag-NPs in 100 ± 5 g freshwater rohu Labeo rohita. The stock fish were
placed in tanks with continuous water supply. There were three treatments and a
control group, each with three replicates comprising five fish each. The treatments
were: garlic only, Ag-NPs only, and garlic plus Ag-NPs. The Ag-NPs (30 mg/L) was
supplied in water, whereas garlic oil (50 µl/kg) was given to the fish through a
prepared feed. The treatment duration was 28 days. In the control and garlic oil
treatment there were no observed alterations in fish. The Ag-NP treatment initiated
inflammation and necrosis in hepatic parenchyma, which resulted in venous
congestion. Garlic oil treatment reduced vascular congestion in hepatic tissues.
Ag-NPs also induced cellular vacuolization and the build-up of a yellow pigment.
Inflammatory gill filament cartilage and lamellae were observed, as were swelling
and collapse of lamellae, accumulation of macrophages, and necrosis and fusion of
secondary lamellae in gill sections of Ag-NP-treated fish. Garlic oil ameliorated
these changes, although some alterations like fused secondary lamellae, separation
between the epithelium and lamellae, and accumulation of macrophages were still
recorded in gill sections of garlic-treated fish. Garlic oil increased the activity
of antioxidants (superoxide dismutase, catalase, glutathione S-transferase and
glutathione) and caused a reduction in lipid peroxidation and/or MDA content.
Furthermore, garlic oil treatment also improved measured hematologic parameters.
This study confirms the role of garlic as a natural antioxidant for the
amelioration of oxidative stress and histological and hemotologic changes in fish
exposed to Ag-NPs. © 2020, Japanese Society of Fisheries Science.
AU - Khan, M. S.
AU - Qureshi, N. A.
AU - Jabeen, F.
AU - Wajid, M.
AU - Sabri, S.
AU - Shakir, M.
DB - Scopus
DO - 10.1007/s12562-020-01403-7
IS - 2
KW - Allium sativum
Colloidal silver
Free radical scavenging
Garlic oil
Nanoparticles
Nanopollution
Oxidative stress
Silver toxicity
M3 - Article
PY - 2020
SP - 255-269
ST - The role of garlic oil in the amelioration of oxidative stress and tissue
damage in rohu Labeo rohita treated with silver nanoparticles
T2 - Fisheries Science
TI - The role of garlic oil in the amelioration of oxidative stress and tissue
damage in rohu Labeo rohita treated with silver nanoparticles
85080939614&doi=10.1007%2fs12562-020-01403-
7&partnerID=40&md5=0897e8b3d24c0182ca77bcb74e53d806
VL - 86
ID - 5306
ER -
TY - JOUR
AB - The development of multifunctional biomaterials for wound dressings,
bandages, tissue scaffolds, etc., has received massive attention in the biomedical
field in the last few years. The advanced progress in tissue engineering is the
fabrication of smart biomaterials mimicking extracellular matrix (ECM) of human
tissue, such as nanofibrous meshes with a micro and nanoporous structure. Silk
fibroin (SF) nanofiber is an ideal candidate as a vehicle/substitute material for
biomedical utilization due to its several unique properties, including high
biocompatibility, biodegradability, and deficient inflammatory reactions. These
characteristics make SF a favorable matrix for therapeutic agents. However,
unmodified SF could be oxidized quickly, exhibit hydrophobicity, making it
inappropriate for biological applications, and inclined to microbial attacks,
lessening its applicability. Intensive research is going on to extend the
capabilities of SF from hydrophobic material to hydrophilic, from the filament to
film, sheet, and scaffolds, and even from soft material to super-rigid material.
The researchers have traversed multiple approaches to remodel SF nanofibers to
improve the utilization of SF. The threat of infections is inevitable when using SF
as wound healing dressing elements or artificial grafts in tissue engineering
applications. To confer antimicrobial property to SF, the use of silver
nanoparticles and other silver composites is one of the strategies. Silver is a
natural antimicrobial agent and has been used since ancient times. Researchers have
incorporated silver in the SF nanofibers to impart antimicrobial properties and
improve their applications. This review describes the extraction process of SF from
silkworms using different methods and their comparative analysis. The
electrospinning of SF nanofibers and the important parameters that need to be
considered during the fabrication of electrospun SF nanofibers has also been
discussed. Furthermore, this review has focused on the current progress in
improvising SF nanofiber utilizing silver nanoparticles for antibacterial and wound
healing applications. © 2022 Elsevier Ltd
AU - Khan, R. S.
AU - Rather, A. H.
AU - Wani, T. U.
AU - Rather, S. U.
AU - Abdal-hay, A.
AU - Sheikh, F. A.
C7 - 103914
DB - Scopus
DO - 10.1016/j.mtcomm.2022.103914
KW - Electrospinning
Nanofiber
Silk fibroin
Tissue engineering
Wound healing
Biocompatibility
Biodegradability
Hydrophobicity
Metal nanoparticles
Microorganisms
Scaffolds (biology)
Tissue
Biomedical fields
Extracellular matrices
Smart biomaterials
Tissue scaffolds
Tissues engineerings
Wound dressings
Nanofibers
M3 - Review
PY - 2022
ST - A comparative review on silk fibroin nanofibers encasing the silver
nanoparticles as antimicrobial agents for wound healing applications
T2 - Materials Today Communications
TI - A comparative review on silk fibroin nanofibers encasing the silver
nanoparticles as antimicrobial agents for wound healing applications
85133933992&doi=10.1016%2fj.mtcomm.2022.103914&partnerID=40&md5=891537f9a5aac03c887
de155edb82016
VL - 32
ID - 5088
ER -
TY - JOUR
AB - Nanoparticles are emerging as a useful tool for a wide variety of biomedical,
consumer and instrumental applications that include drug delivery systems,
biosensors and environmental sensors. In particular, nanoparticles have been shown
to offer greater specificity with enhanced bioavailability and less detrimental
side effects as compared to the existing conventional therapies in nanomedicine.
Hence, bionanotechnology has been receiving immense attention in recent years.
However, despite the extensive use of nanoparticles today, there is still a limited
understanding of nanoparticle-mediated toxicity. Both in vivo and in vitro studies
have shown that nanoparticles are closely associated with toxicity by increasing
intracellular reactive oxygen species (ROS) levels and/or the levels of pro-
inflammatory mediators. The homeostatic redox state of the host becomes disrupted
upon ROS induction by nanoparticles. Nanoparticles are also known to up-regulate
the transcription of various pro-inflammatory genes, including tumor necrosis
factor-alpha and IL (interleukins)-1, IL-6 and IL-8, by activating nuclear factor-
kappa B (NF-kappa B) signaling. These sequential molecular and cellular events are
known to cause oxidative stress, followed by severe cellular genotoxicity and then
programmed cell death. However, the exact molecular mechanisms underlying
nanotoxicity are not fully understood. This lack of knowledge is a significant
impediment in the use of nanoparticles in vivo. In this review, we will provide an
assessment of signaling pathways that are involved in the nanoparticle-induced
oxidative stress and propose possible strategies to circumvent nanotoxicity.
AN - WOS:000362643900003
AU - Khanna, P.
AU - Ong, C.
AU - Bay, B. H.
AU - Baeg, G. H.
DA - SEP
DO - 10.3390/nano5031163
IS - 3
PY - 2015
SN - 2079-4991
SP - 1163-1180
ST - Nanotoxicity: An Interplay of Oxidative Stress, Inflammation and Cell Death
T2 - NANOMATERIALS
TI - Nanotoxicity: An Interplay of Oxidative Stress, Inflammation and Cell Death
VL - 5
ID - 6513
ER -
TY - JOUR
AB - Nanotechnology has vast applications in almost all fields of science and
technology. The use of medicinal plants for the synthesis of metallic nanoparticles
has gained much attention nowadays. In the current research work, six medicinal
plants were used for the synthesis of gold nanoparticles (AuNPs) and iron
nanoparticles (FeNPs). The synthesized nanoparticles were characterized by
different techniques including UV-visible spectrophotometry, scanning electron
microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). Furthermore,
the activities of green synthesized nanoparticles were screened in vitro using, for
example, antibacterial, antioxidant, cytotoxic, and DNA protection assays. Both
FeNPs and AuNPs had spherical shapes with an average size less than 50 nm and were
found to have good antimicrobial and nontoxic effects. Furthermore, FeNPs from
Ficus microcarpa demonstrated high drug loading efficiency (65%) as compared to an
anti-inflammatory drug (diclofenac potassium, DFP). We also evaluated the drug
delivery potential, as well as anti-inflammatory and anticoagulant properties, of
nanoparticles in vivo. Interestingly, AuNPs of Syzygium cumini exhibited strong
anti-inflammatory potential as compared to DFP and diclofenac loaded FeNPs of Ficus
microcarpa. The results suggest potential pharmacological applications of biogenic
synthesized AuNPs and FeNPs which can be explored further. The study revealed that
the green synthesized AuNPs and FeNPs provide a promising approach for the
synthesis of drug-loaded nanoparticles and consequently in the field of targeted
drug delivery.
AN - WOS:000879579700001
AU - Khanzada, B.
AU - Akthar, N.
AU - Bhatti, M. Z.
AU - Ismail, H.
AU - Alqarni, M.
AU - Mirza, B.
AU - Mostafa-Hedeab, G.
AU - Batiha, G. E. S.
C7 - 1581444
DA - NOV 23
DO - 10.1155/2021/1581444
PY - 2021
SN - 2090-9063
2090-9071
ST - Green Synthesis of Gold and Iron Nanoparticles for Targeted Delivery: An In
Vitro and In Vivo Study
T2 - JOURNAL OF CHEMISTRY
TI - Green Synthesis of Gold and Iron Nanoparticles for Targeted Delivery: An In
Vitro and In Vivo Study
VL - 2021
ID - 6625
ER -
TY - JOUR
AB - Introduction: Herbs are excellent sources of medicinal substances, and their
curative abilities have been recognized to treat many ailments and are used for
example as antioxidants, analgesics, anti-inflammatories, antipyretics, and many
other medicinal uses. The properties of natural compounds and their health effects
have been studied extensively, especially those that originate from plant sources
such as ginger. The ginger plant contains many chemical compounds, such as 6-
gingerol, which is characterized by containing active groups such as carbonyl and
hydroxide, which can be attached to metal molecules. This is what was done in this
study, where the formation of complexes with a group of metals was studied and
their effect on cancer cells was investigated. These complexes will open new
horizons for further study of medicinal uses. Methods: The synthesis of gingerol-
metal complexes was carried out by conjugating gingerol molecules with Ag, Au, Cd,
Co, Cu, Ni, and Zn metal ions. The extracted gingerol was transferred to culture
tubes and deionized water-DMSO were added followed by sonication. The tubes were
incubated at 90°C for two days as well as the control sample. The samples were then
filtered and the complex solutions were transferred into new tubes for further
studies. Different characterization techniques such as FT-IR, UVvis spectroscopy,
FESEM, and EDX are used to confirm the formation of the complexes. The in vitro of
the complexes was tested by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-
carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay against the
human colorectal cancer cell lines HCT116 and HT29 which exhibited strong
cytotoxicity. Results: The gingerol-metal complexes showed an enhancement as an
anticancer agent compared to the control. The in vitro anticancer activity showed
that the Ag-gingerol complex showed the most activity among the other complexes.
Discussion: Gingerol-metal complexes can inhibit cancer cells, noting that the
potency of the complex depends on the type of metal used. © 2023 Khdary et al.
AU - Khdary, N. H.
AU - Alangari, A. A.
AU - Katubi, K. M.
AU - Alanazi, M.
AU - Alhassan, A.
AU - Alzahrani, S. D.
AU - Khan, Z.
AU - Alanazi, I. O.
DB - Scopus
DO - 10.2147/CMAR.S391546
KW - Colon cancer
Ginger
Gingerol
Gingerol-complexes
Tumor cell line
analgesic agent
antioxidant
antipyretic agent
cadmium
carbonyl derivative
cobalt
copper
gingerol
gold
hydroxide
metal complex
natural product
nickel
silver
zinc
Article
cell viability
colon cancer cell line
controlled study
cytotoxicity
drug synthesis
elemental analysis
HCT 116 cell line
herb
HT-29 cell line
human
human cell
IC50
in vitro study
MTS assay
M3 - Article
PY - 2023
SP - 87-98
ST - Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human
Colon Cancer Cell Line
T2 - Cancer Management and Research
TI - Synthesis of Gingerol-Metals Complex and in-vitro Cytotoxic Activity on Human
Colon Cancer Cell Line
85148702475&doi=10.2147%2fCMAR.S391546&partnerID=40&md5=7f5ee89c182c394b3548ad1d169
ef269
VL - 15
ID - 5009
ER -
TY - JOUR
AB - Toxic chemicals are used in traditional nanoparticle synthesis processes,
resulting in environmental toxicity. As a result, we must switch to "green
synthesis." Previous studies have shown that Silver nanoparticles reinforced with
Amla extract have excellent antimicrobial properties. As a result, this research
was carried out to determine the anti-inflammatory and antioxidant properties of
Silver. Since Silver induces cell wall lysis due to intracellular material leakage,
it is an excellent antimicrobial agent and was chosen to make a nanoparticle. Aim:
Aim of the study was phyto assisted preparation of Silver nanoparticles from Amla
fruit and evaluation of its anti-inflammatory and antioxidant properties. Material
and methods: Anti-inflammatory and antioxidant properties of the nanoparticle were
assessed using Bovine Serum Albumin (BSA) and DPPH Assay respectively at 10 mu L,
20 mu L, 30 mu L, 40 mu L, 50 mu L. Results: Values for anti-inflammatory property
of nanoparticles were higher than the standard values at 40 mu L, 50 mu L
concentrations. Percentage of inhibition was highest at 40 mu L (86%) and 50 mu L
(84.6%). The values for antioxidant property of nanoparticles were found to be
higher than the standard values at concentrations except at 40 mu L, 50 mu L.
Percentage of inhibition was highest at 20 mu L (86.2%) Conclusion: Within the
limits of the study it can be concluded that Silver nanoparticles have exceptional
antiinflammatory and antioxidant properties and further can be incorporated in
dental material or can be used to coat suture materials to improve their
properties.
AN - WOS:000862589200015
AU - Khullar, D.
AU - Ahmed, N.
IS - 8
PY - 2022
SN - 2347-2545
2347-2367
SP - 47-+
ST - Green Synthesis, Characterization and Assessment of Anti-inflammatory and
Antioxidant Properties of Silver Nanoparticles Prepared using Amla Extracts-An In-
vitro Study
T2 - JOURNAL OF RESEARCH IN MEDICAL AND DENTAL SCIENCE
TI - Green Synthesis, Characterization and Assessment of Anti-inflammatory and
Antioxidant Properties of Silver Nanoparticles Prepared using Amla Extracts-An In-
vitro Study
VL - 10
ID - 5851
ER -
TY - JOUR
AB - Hemorrhage in mice results in decreased ATP levels in the jejunum, lung,
kidney, heart, and brain but not in liver tissue lysates, albeit at variable levels
and time kinetics. The decreased protein expression and activity of pyruvate
dehydrogenase ( PDH) accounted for the hemorrhage-induced ATP loss. Treatment with
geldanamycin (GA; 1 mu g/g body wt), a known inducer of heat shock protein (HSP)70,
inhibited the hemorrhage-induced ATP loss in the jejunum, lung, heart, kidney, and
brain. GA was found to increase PDH protein, preserve PDH enzymatic activity, and
inhibit mucosal injury in jejunum tissues. GA-induced HSP70i was found to form
complexes with PDH protein. HSP70 gene transfer into intestinal epithelial cells
promoted PDH and ATP levels, whereas HSP70 short interfering RNA limited them. We
conclude that agents able to increase the expression of HSP70 and PDH may be of
value in reducing pathology resulting from hemorrhage-associated ATP loss.
AN - WOS:000238287600015
AU - Kiang, J. G.
AU - Bowman, P. D.
AU - Lu, X. Y.
AU - Li, Y. S.
AU - Ding, X. Z.
AU - Zhao, B. T.
AU - Juang, Y. T.
AU - Atkins, J. L.
AU - Tsokos, G. C.
DA - JUL
DO - 10.1152/ajpgi.00397.2005
IS - 1
PY - 2006
SN - 0193-1857
1522-1547
SP - G117-G127
ST - Geldanamycin prevents hemorrhage-induced ATP loss by overexpressing inducible
HSP70 and activating pyruvate dehydrogenase
T2 - AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
TI - Geldanamycin prevents hemorrhage-induced ATP loss by overexpressing inducible
HSP70 and activating pyruvate dehydrogenase
VL - 291
ID - 6834
ER -
TY - JOUR
AB - Soft tissue trauma and hemorrhage (T-H) diminishes various aspects of liver
function, while it increases hepatic nitrate/nitrite, inducible nitric oxide
synthase (iNOS), and endothelin-1 levels. Treatment with androstenediol (AED)
inhibits the T-H-induced alterations of the above parameters. We sought to identify
the molecular events underlying the beneficial effect of AED. Exposure of rats to
T-H significantly increased the caspase-3 activity and protein, whereas treatment
with AED significantly limited these increases. AED treatment also suppressed the
T-H-induced increase in iNOS by effectively altering the levels of key
transcription factors involved in the regulation of iNOS expression.
Immunoprecipitation and immunoblotting analyses indicate that T-H increased
apoptosome formation, and AED treatment significantly decreased it. Modulating the
iNOS protein by transfecting cells with iNOS gene or small interfering RNA further
confirmed the correlation between iNOS and caspase-3. Our data indicate that AED
limits caspase-3 expression by suppressing the expression of transcription factors
involved in the production of iNOS, resulting in decreased apoptosome. AED can
potentially be a useful adjuvant for limiting liver apoptosis following T-H shock.
AN - WOS:000244722400017
AU - Kiang, J. G.
AU - Peckham, R. M.
AU - Duke, L. E.
AU - Shimizu, T.
AU - Chaudry, I. H.
AU - Tsokos, G. C.
DA - MAR
DO - 10.1152/japplphysiol.00919.2006
IS - 3
PY - 2007
SN - 8750-7587
1522-1601
SP - 933-941
ST - Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3
by downregulating the inducible nitric oxide synthase pathway
T2 - JOURNAL OF APPLIED PHYSIOLOGY
TI - Androstenediol inhibits the trauma-hemorrhage-induced increase in caspase-3
by downregulating the inducible nitric oxide synthase pathway
VL - 102
ID - 6802
ER -
TY - JOUR
AB - Background and Purpose Abnormally induced angiogenesis and lymphangiogenesis
are associated with human diseases, including neovascular eye disease. Substances
that inhibit these processes may have potential as an attractive therapeutic
strategy for these diseases. Experimental Approach In vitro and in vivo
angiogenesis and/or lymphangiogenesis were assessed in VEGF- or hypoxia-stimulated
endothelial and retinal cells and in animal models of oxygen-induced retinopathy
(OIR), streptozotocin-induced diabetic retinopathy (SIDR), suture-induced
inflammatory corneal neovascularization (SICNV) and silver nitrate-induced corneal
neovascularization. HUVECs and retinal cells were cultured under hypoxic conditions
or incubated with VEGF to identify the molecular mechanisms involved. Key Results
The imidazole-based alkaloid derivative LCB54-0009 inhibited capillary-like tube
formation in VEGF-induced HUVECs without inducing cytotoxic effects. Intravitreal
injection of LCB54-0009 into retinas suppressed the formation of the pathological
neovascular tufts and increased vascular permeability in both OIR of mice and SIDR
of rats. Furthermore, subconjunctival injection of LCB54-0009 into the cornea
suppressed corneal inflammation and inflammation-associated angiogenesis and
lymphangiogenesis in SICNV of mice and silver nitrate cauterization of rats. These
pharmacological activities were associated with effects on HIF-1α protein stability
and HIF-1α/NF-κB redox sensitivity through its antioxidant activities. LCB54-0009
also inhibited the hypoxia-induced expression of angiopoietin-2, and VEGF-induced
VEGFR-2 activation and downstream signalling, resulting in the down-regulation of
the expression of pro-angiogenic factors and pro-inflammatory mediators and an up-
regulation of the expression of anti-angiogenic factors. Conclusions and
Implications LCB54-0009 is a potential candidate molecule for blocking pathological
angiogenesis and lymphangiogenesis mediated by HIF-1α- angiopoietin-2 expression
and VEGFR-2 activation. © 2015 The British Pharmacological Society.
AU - Kim, B. H.
AU - Lee, J.
AU - Choi, J. S.
AU - Park, D. Y.
AU - Song, H. Y.
AU - Park, T. K.
AU - Cho, C. H.
AU - Ye, S. K.
AU - Joo, C. K.
AU - Koh, G. Y.
AU - Kim, T. Y.
DB - Scopus
DO - 10.1111/bph.13177
IS - 15
KW - Alkaloids
Angiogenesis Inhibitors
Angiopoietin-2
Animals
Benzodioxoles
Corneal Neovascularization
Diabetic Retinopathy
Down-Regulation
Humans
Hypoxia
Hypoxia-Inducible Factor 1, alpha Subunit
Imidazoles
Lymphangiogenesis
Mice
Neovascularization, Pathologic
NF-kappa B
Oxygen
Rats
Retina
Silver Nitrate
Up-Regulation
Vascular Endothelial Growth Factor Receptor-2
3 [2 [[3,4 dihydroxy phenyl] [4 [2 dimethylamino ethyl] imidazol 1 yl] methyl]
benzo[1,3]dioxol 5 yl] propionic acid
alkaloid derivative
angiopoietin 2
hypoxia inducible factor 1alpha
oxygen
silver nitrate
streptozocin
unclassified drug
vasculotropin
1,3 benzodioxole derivative
3-(2-((3,4-dihydroxyphenyl)-(4-(2-dimethylaminoethyl)imidazol-1-
yl)methyl)benzo(1,3)dioxol-5-yl)propionic acid
alkaloid
angiogenesis inhibitor
vasculotropin A
angiogenesis
animal experiment
animal model
animal tissue
Article
cell culture
cell stimulation
controlled study
cornea neovascularization
cytotoxicity
diabetic retinopathy
down regulation
drug structure
endothelium cell
experimental retinopathy
human
human cell
hypoxia
in vitro study
in vivo study
inflammation
keratitis
lymphangiogenesis
male
mouse
nonhuman
ocular blood vessel
oxidation reduction state
priority journal
protein expression
protein stability
rat
retina cell
signal transduction
suture
umbilical vein endothelial cell
upregulation
animal
chemically induced
disease model
drug effects
metabolism
retina
synthesis
M3 - Article
PY - 2015
SP - 3875-3889
ST - Imidazole-based alkaloid derivative LCB54-0009 suppresses ocular angiogenesis
and lymphangiogenesis in models of experimental retinopathy and corneal
neovascularization
T2 - British Journal of Pharmacology
TI - Imidazole-based alkaloid derivative LCB54-0009 suppresses ocular angiogenesis
and lymphangiogenesis in models of experimental retinopathy and corneal
neovascularization
84936991254&doi=10.1111%2fbph.13177&partnerID=40&md5=689eac41dee65b3b4c03c7fd360d39
3f
VL - 172
ID - 5603
ER -
TY - JOUR
AB - Biomonitoring of workers is an approach of evaluating workers' exposure to
chemicals and particulate matter by measuring biomarkers of parent chemicals, their
metabolites, and reaction products in workers' biospecimens. Prerequisites for
biological monitoring in the workplace include permission to enter the workplace,
approval of the study plan from the IRB (Institutional Review Board), and obtaining
consent from workers. Because of the complex legal process involved in
biomonitoring, few studies have been conducted so far on biomonitoring of workers'
exposures to nanoparticles and other hazards from emerging materials and advanced
nanotechnologies. We have developed a cell-based biomonitoring device that can
evaluate acute cytotoxicity and various other effect biomakers, such as
inflammation, at realistic workplace exposure. This device is based on air-liquid
interphase (ALI) and can be used to evaluate cell toxicity and early effect
biomarkers along adverse outcome pathways. Following exposure of A549 lung
epithelial cells in ALI to workplace air for 1-2 h, the cells were processed to
assess the induction of inflammatory and cell damage biomarkers. Initially, we
estimated the deposition rate of nanoparticles in the transwell by exposing the
cell-free ALI device to silver nanoparticle aerosols (AgNP 20-30 nm) for 2 h in the
laboratory. Then A549 lung epithelial cells cultured on the transwell in the ALI
device were exposed to AgNP nanoaerosols for 2 h and evaluated for cytotoxicity and
induction of mRNAs of pro-inflammatory cytokines IL-1b, IL-6, and TNF-alpha. Then
the cells in the ALI device were exposed to 3-D printer emissions at the workplace
and evaluated for the same matched endpoints. The mRNA levels for IL-1b, IL-6, and
TNF alpha a increased significantly at the end of 2-h exposure of A549 cells to the
positive control AgNP aerosols. These mRNAs, as well as LDH and microprotein
concentrations, increased even more after 24-h post-exposure incubation (p < 0.05).
Cytotoxicity evaluation of 3-D printer emissions at 810 and 957 mu g/m(3), which
was more than 80 times higher than the airborne total suspended particulate
concentrations in the workplace air (9-12.5 mu g/m(3)), suggested no significant
acute cytotoxicity at the end of 2-h exposure to 3-D-printing emission, as well as
at 24-h post-exposure incubation. Hyperspectral microscopic observation showed that
3-D printers emitted particles to be attached to A549 cells after 2-h exposure, and
many particles were internalized by A549 cells after 24 h of post-exposure
incubation. The mRNA expression of pro-inflammatory cytokine IL-1b and IL-6
increased significantly after 2-h exposure to 3-D printer emissions and after 24-h
incubation (only IL-6). In contrast, the expression of TNF-alpha mRNA decreased
significantly after 2 h of exposure to 3-D printers and decreased even more after
24-h post-exposure incubation. These results support the use of cell-based ALI
devices for direct assessment of airborne hazards in the workplace, for probing
toxicological properties of airborne contaminants using adverse molecular pathways,
and for guiding study design for workplace biomonitoring. ALI devices can bridge
conventional exposure assessment with cellular toxicity testing platforms for
hazard and risk assessment.
AN - WOS:001005190000001
AU - Kim, B.
AU - Shin, J. H.
AU - Kim, H. P.
AU - Jo, M. S.
AU - Kim, H. S.
AU - Lee, J. S.
AU - Lee, H. K.
AU - Kwon, H. C.
AU - Han, S. G.
AU - Kang, N. L.
AU - Gulumian, M.
AU - Bello, D.
AU - Yu, I. J.
C7 - 818942
DA - MAR 25
DO - 10.3389/ftox.2022.818942
PY - 2022
SN - 2673-3080
ST - On-Site Deployment of an Air-Liquid-Interphase Device to Assess Health Hazard
Potency of Airborne Workplace Contaminants: The Case of 3-D Printers
T2 - FRONTIERS IN TOXICOLOGY
TI - On-Site Deployment of an Air-Liquid-Interphase Device to Assess Health Hazard
VL - 4
ID - 6361
ER -
TY - JOUR
AB - Biomonitoring of workers is an approach of evaluating workers’ exposure to
chemicals and particulate matter by measuring biomarkers of parent chemicals, their
metabolites, and reaction products in workers’ biospecimens. Prerequisites for
biological monitoring in the workplace include permission to enter the workplace,
approval of the study plan from the IRB (Institutional Review Board), and obtaining
consent from workers. Because of the complex legal process involved in
biomonitoring, few studies have been conducted so far on biomonitoring of workers’
exposures to nanoparticles and other hazards from emerging materials and advanced
nanotechnologies. We have developed a cell-based biomonitoring device that can
evaluate acute cytotoxicity and various other effect biomakers, such as
inflammation, at realistic workplace exposure. This device is based on air–liquid
interphase (ALI) and can be used to evaluate cell toxicity and early effect
biomarkers along adverse outcome pathways. Following exposure of A549 lung
epithelial cells in ALI to workplace air for 1–2 h, the cells were processed to
assess the induction of inflammatory and cell damage biomarkers. Initially, we
estimated the deposition rate of nanoparticles in the transwell by exposing the
cell-free ALI device to silver nanoparticle aerosols (AgNP 20–30 nm) for 2 h in the
laboratory. Then A549 lung epithelial cells cultured on the transwell in the ALI
device were exposed to AgNP nanoaerosols for 2 h and evaluated for cytotoxicity and
induction of mRNAs of pro-inflammatory cytokines IL-1b, IL-6, and TNF-α. Then the
cells in the ALI device were exposed to 3-D printer emissions at the workplace and
evaluated for the same matched endpoints. The mRNA levels for IL-1b, IL-6, and TNF-
α increased significantly at the end of 2-h exposure of A549 cells to the positive
control AgNP aerosols. These mRNAs, as well as LDH and microprotein concentrations,
increased even more after 24-h post-exposure incubation (p < 0.05). Cytotoxicity
evaluation of 3-D printer emissions at 810 and 957 μg/m3, which was more than 80
times higher than the airborne total suspended particulate concentrations in the
workplace air (9–12.5 μg/m3), suggested no significant acute cytotoxicity at the
end of 2-h exposure to 3-D-printing emission, as well as at 24-h post-exposure
incubation. Hyperspectral microscopic observation showed that 3-D printers emitted
particles to be attached to A549 cells after 2-h exposure, and many particles were
internalized by A549 cells after 24 h of post-exposure incubation. The mRNA
expression of pro-inflammatory cytokine IL-1b and IL-6 increased significantly
after 2-h exposure to 3-D printer emissions and after 24-h incubation (only IL-6).
In contrast, the expression of TNF-α mRNA decreased significantly after 2 h of
exposure to 3-D printers and decreased even more after 24-h post-exposure
incubation. These results support the use of cell-based ALI devices for direct
assessment of airborne hazards in the workplace, for probing toxicological
properties of airborne contaminants using adverse molecular pathways, and for
guiding study design for workplace biomonitoring. ALI devices can bridge
conventional exposure assessment with cellular toxicity testing platforms for
hazard and risk assessment. Copyright © 2022 Kim, Shin, Kim, Jo, Kim, Lee, Lee,
Kwon, Han, Kang, Gulumian, Bello and Yu.
AU - Kim, B.
AU - Shin, J. H.
AU - Kim, H. P.
AU - Jo, M. S.
AU - Kim, H. S.
AU - Lee, J. S.
AU - Lee, H. K.
AU - Kwon, H. C.
AU - Han, S. G.
AU - Kang, N.
AU - Gulumian, M.
AU - Bello, D.
AU - Yu, I. J.
C7 - 818942
DB - Scopus
DO - 10.3389/ftox.2022.818942
KW - 3-D printer emission
air-liquid-interphase
biomonitoring
inflammasome
TNF-α mRNA
workplace
M3 - Article
PY - 2022
ST - On-Site Deployment of an Air-Liquid-Interphase Device to Assess Health Hazard
T2 - Frontiers in Toxicology
TI - On-Site Deployment of an Air-Liquid-Interphase Device to Assess Health Hazard
85152405395&doi=10.3389%2fftox.2022.818942&partnerID=40&md5=575a79d364d9643bb89ca81
17efb1848
VL - 4
ID - 5084
ER -
TY - JOUR
AB - Today nanosciences are experiencing massive investment worldwide although
research on toxicological aspect of these nano-sized particles has just begun and
to date, no clear guidelines exist to quantify the effects. In the present study,
we focus on silver nanoparticles, which represent one of the most widely
investigated nanoparticles. The present data indicate that silver nanoparticles
seem to cross the cellular membrane of various tissues in Sprague Dawley rat and,
therefore, might have an influence on cell physiology and function. Rats are
exposed via oral administration and intravenous injection with commercial silver
nanoparticles. Three types of silver nanoparticels are used in this study: 1) Type
I, particle size 50-90 nm with no dispersant, 2) Type II, particle size 1-10 nm
dispersed with several amino acids. After 4 weeks exposure we examined the clinical
indicators from blood and also analyzed histological changes in various tissues,
including liver, kidney, and lung, to investigate the histopathological changes.
The concentration of 2 indicators, total cholesterol and creatinine were changed
with statistical importance. Also, lymphocytes/granulocyte ratio was significantly
increased by silver nanoparticles. The histological change had accordance with the
change of clinical indicators. The inflammatory symptoms were observed in liver
tissue and it lead to the result that the hematologic/lymphocytic disorder, not
hepatic disorder, would be related to silver nanoparticle toxicity. Finally we
propose 4 genes as size independent genomic biomarkers and 10 genes as
representative biomarkers for histopathological and clinical changes for silver
nanoparticle exposure. © 2009 The Korean Society of Environmental Risk Assessment
and Health Science and Springer.
AU - Kim, E.
AU - Maeng, J. H.
AU - Lee, D. H.
AU - Kim, J. M.
DB - Scopus
DO - 10.1007/BF03216458
IS - 1
KW - Biomarker
Silver nanoparticle
Toxicogenomics
M3 - Article
PY - 2009
SP - 8-16
ST - Correlation of biomarkers and histological responses in manufactured silver
nanoparticle toxicity
T2 - Toxicology and Environmental Health Sciences
TI - Correlation of biomarkers and histological responses in manufactured silver
nanoparticle toxicity
84873250628&doi=10.1007%2fBF03216458&partnerID=40&md5=d13fb4cbbaa5d27b99aebe5f400e1
918
VL - 1
ID - 5702
ER -
TY - JOUR
AB - Background: Scutellaria baicalensis Georgi is a commonly used medicinal herb
in several Asian countries like Korea, China and Japan for thousands of years. It
has been reported to have various medicinal properties such as anti-microbial,
anti-inflammatory and anti-cancer effects. However, the anti-inflammatory mechanism
of S. baicalensis G at proteome level has not yet been reported. Hence, we
performed a proteome analysis to study differentially expressed proteins and its
anti-inflammatory role in lipopolysaccharide (LPS) stimulated L6 skeletal muscle
cells response to flavonoids isolated from S. baicalensis G. Methods: For that, 150
mu g of proteins from the L6 cells of the control (Vehicle only), LPS treated and
flavonoid treated groups were separated using 18 cm, pH 4-7 IPG strips in the first
dimension and resolved by 12% linear gradient SDS-polyacrylamide gel
electrophoresis (SDS-PAGE). The silver stained gels were analyzed by using
progenesis SameSpots software and twenty six differentially expressed protein spots
(>= 2 fold, p < 0.05) were selected for matrix assisted laser desorption
ionization-time of flight mass spectroscopy/mass spectrometry (MALDI-TOF/MS)
analysis. Also, the expression of COX-2, iNOS and Annexin A2 proteins were analyzed
by western blot. Results: Totally, 12 differentially expressed proteins were
successfully identified by MALDI-TOF/MS and database searching, that's involved in
inflammatory responses such vimentin, T-box transcription factor TBX3, annexin A1,
annexin A2 and annexin A5. In addition, flavonoids inhibited the expression of COX-
2, iNOS and Annexin A2 proteins in LPS-stimulated L6 skeletal muscle cells.
Conclusions: The findings revealed that the flavonoids from S. baicalensis G.
directly protect the LPS stimulated inflammation process in L6 cells and, would be
helpful to study further the muscle cell inflammatory mechanism. This is the first
proteome study provide the anti-inflammatory mechanism of flavonoids from S.
baicalensis G. in LPS stimulated L6 skeletal muscle cells.
AN - WOS:000345800400001
AU - Kim, J. A.
AU - Nagappan, A.
AU - Park, H. S.
AU - Saralamma, V. V. G.
AU - Hong, G. E.
AU - Yumnam, S.
AU - Lee, H. J.
AU - Raha, S.
AU - Kim, E. H.
AU - Young, P. S.
AU - Kim, G. S.
C7 - 379
DA - OCT 7
DO - 10.1186/1472-6882-14-379
PY - 2014
SN - 1472-6882
ST - Proteome profiling of lipopolysaccharide induced L6 rat skeletal muscle cells
response to flavonoids from Scutellaria baicalensis Georgi
T2 - BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
TI - Proteome profiling of lipopolysaccharide induced L6 rat skeletal muscle cells
VL - 14
ID - 6424
ER -
TY - JOUR
AB - Background: Scutellaria baicalensis Georgi is a commonly used medicinal herb
in several Asian countries like Korea, China and Japan for thousands of years. It
has been reported to have various medicinal properties such as anti-microbial,
anti-inflammatory and anti-cancer effects. However, the anti-inflammatory mechanism
of S. baicalensis G at proteome level has not yet been reported. Hence, we
performed a proteome analysis to study differentially expressed proteins and its
anti-inflammatory role in lipopolysaccharide (LPS) stimulated L6 skeletal muscle
cells response to flavonoids isolated from S. baicalensis G.Methods: For that,
150 μg of proteins from the L6 cells of the control (Vehicle only), LPS treated and
flavonoid treated groups were separated using 18 cm, pH 4-7 IPG strips in the first
dimension and resolved by 12% linear gradient SDS-polyacrylamide gel
electrophoresis (SDS-PAGE). The silver stained gels were analyzed by using
progenesis SameSpots software and twenty six differentially expressed protein spots
(≥2 fold, p < 0.05) were selected for matrix assisted laser desorption ionization-
time of flight mass spectroscopy/mass spectrometry (MALDI-TOF/MS) analysis. Also,
the expression of COX-2, iNOS and Annexin A2 proteins were analyzed by western
blot.Results: Totally, 12 differentially expressed proteins were successfully
identified by MALDI-TOF/MS and database searching, that's involved in inflammatory
responses such vimentin, T-box transcription factor TBX3, annexin A1, annexin A2
and annexin A5. In addition, flavonoids inhibited the expression of COX-2, iNOS and
Annexin A2 proteins in LPS-stimulated L6 skeletal muscle cells.Conclusions: The
findings revealed that the flavonoids from S. baicalensis G. directly protect the
LPS stimulated inflammation process in L6 cells and, would be helpful to study
further the muscle cell inflammatory mechanism. This is the first proteome study
provide the anti-inflammatory mechanism of flavonoids from S. baicalensis G. in LPS
stimulated L6 skeletal muscle cells. © 2014 Kim et al.; licensee BioMed Central
Ltd.
AU - Kim, J. A.
AU - Nagappan, A.
AU - Park, H. S.
AU - Venkatarame Gowda Saralamma, V.
AU - Hong, G. E.
AU - Yumnam, S.
AU - Lee, H. J.
AU - Raha, S.
AU - Kim, E. H.
AU - Young, P. S.
AU - Kim, G. S.
C7 - 379
DB - Scopus
DO - 10.1186/1472-6882-14-379
IS - 1
KW - Flavonoids
L6 skeletal muscle cells
Lipopolysaccharide (LPS)
Matrix assisted laser desorption ionization time of flight mass spectrometry
(MALDI-TOF/MS)
Scutellaria baicalensis G
Two dimensional gel electrophoresis
Animals
Cell Line
Cell Survival
Lipopolysaccharides
Muscle, Skeletal
Plant Extracts
Proteins
Proteome
Rats
Scutellaria baicalensis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
cyclooxygenase 2
flavonoid
lipocortin 1
lipocortin 2
lipocortin 5
lipopolysaccharide
proteome
Scutellaria baicalensis extract
T box transcription factor
vimentin
plant extract
protein
animal experiment
animal model
Article
controlled study
cytotoxicity
gene ontology
inflammation
nonhuman
protein expression
rat
skeletal muscle
Western blotting
animal
cell line
cell survival
chemistry
cytology
drug effects
mass spectrometry
toxicity
M3 - Article
PY - 2014
ST - Proteome profiling of lipopolysaccharide induced L6 rat skeletal muscle cells
T2 - BMC Complementary and Alternative Medicine
TI - Proteome profiling of lipopolysaccharide induced L6 rat skeletal muscle cells
84910050262&doi=10.1186%2f1472-6882-14-
379&partnerID=40&md5=6b358e66b8ca8bf35d0ee59f092d7242
VL - 14
ID - 5593
ER -
TY - JOUR
AB - Mast cells are responsible for IgE-mediated allergic responses through the
secretion of various inflammatory cytokines and mediators. Therefore, the
pharmacological regulation of mast cell activation is an important goal in the
development of novel anti-allergic drugs. In this study, we found that spiraeoside
(SP) inhibits mast cell activation and allergic responses in vivo. SP dose-
dependently inhibited the degranulation induced by IgE-antigen (Ag) stimulation in
RBL-2H3 mast cells without cytotoxic effects. At the molecular level, SP reduced
the Ag-induced phosphorylation and subsequent activation of phospholipase C-γ2
(PLC-γ2). Moreover, SP inhibited the phosphorylation of spleen tyrosine kinase
(Syk), linker for activation of T cells (LAT), and downstream MAPKs, such as
ERK1/2, p38, and JNK, eventually attenuating expression of TNF-κ and IL-4. Finally,
we found that SP significantly inhibited IgE-mediated passive cutaneous anaphylaxis
(PCA) in mice. Taken together, our results strongly suggest that SP suppresses IgE-
mediated mast cell activation and allergic responses by inhibiting Lyn-induced PLC-
γ2/MAPK signaling in mast cells.
AU - Kim, J. K.
AU - Seo, Y. K.
AU - Park, S.
AU - Park, S. A.
AU - Lim, S.
AU - Lee, S.
AU - Kwon, O.
AU - Seo, J. K.
AU - Choi, U. K.
AU - Ryu, S. H.
AU - Suh, P. G.
DB - Scopus
DO - 10.1139/bcb-2014-0055
IS - 3
KW - allergic response
degranulation
Lyn/Syk kinase
MAPK
mast cells
PLC-γ
Spiraeoside
Animals
Cell Line
Cytokines
Immunoglobulin E
Male
Mast Cells
Mice, Inbred BALB C
Passive Cutaneous Anaphylaxis
Phospholipase C gamma
Phosphorylation
Quercetin
Rats
Signal Transduction
src-Family Kinases
Mus
Amino acids
Cells
Chemical activation
Cytology
Cytotoxicity
Enzymes
Silver
calcium ion
immunoglobulin E
Janus kinase
LAT protein
phospholipase C gamma
protein kinase Syk
RNA
silver
spiraeoside
cytokine
protein tyrosine kinase
quercetin
Allergic response
Degranulation
Mast cells
allergic reaction
allergy
animal cell
animal experiment
Article
cell viability
drug effect
enzyme activation
leukemia cell line
mast cell
mast cell leukemia
mouse
nonhuman
passive skin anaphylaxis
rat
Western blotting
analogs and derivatives
animal
Bagg albino mouse
cell line
drug effects
immunology
male
metabolism
phosphorylation
signal transduction
Cell signaling
M3 - Article
PY - 2015
SP - 227-235
ST - Spiraeoside inhibits mast cells activation and IgE-mediated allergic
responses by suppressing phospholipase C-γ-mediated signaling
T2 - Biochemistry and Cell Biology
TI - Spiraeoside inhibits mast cells activation and IgE-mediated allergic
responses by suppressing phospholipase C-γ-mediated signaling
84946240688&doi=10.1139%2fbcb-2014-
0055&partnerID=40&md5=91a144efccb17ff7a20e5c70d86dbcc6
VL - 93
ID - 5583
ER -
TY - JOUR
AB - There has been a rising interest in the possible association between
perioperative opioid use and postoperative outcomes in cancer patients. Continuous
surgical wound infiltration with local anesthetics is a nonopioid analgesic
technique that can be used as a postoperative pain management alternative to
opioid-based intravenous patient-controlled analgesia (IV PCA). The aim of this
study was to compare the effects of an opioid-based analgesic regimen versus a
local anesthetic wound infiltration-based analgesic regimen on immune modulation
and short-term cancer recurrence or metastasis in patients undergoing laparoscopic
resection of colorectal cancer.Sixty patients undergoing laparoscopic resection of
colorectal cancer were randomly assigned to either the opioid group or the ON-Q
group. For postoperative analgesia during the first 48hours, the opioid group
(n=30) received fentanyl via IV PCA, whereas the ON-Q group (n=30) received
continuous wound infiltration of 0.5% ropivacaine with an ON-Q pump and tramadol
via IV PCA. Pethidine for the opioid group and ketorolac or propacetamol for the
ON-Q group were used as rescue analgesics. Anesthesia was induced and maintained
with propofol and remifentanil. The primary outcome was postoperative immune
function assessed by natural killer cell cytotoxicity (NKCC) and interleukin-2.
Secondary outcomes were postoperative complications, cancer recurrence, or
metastasis within 1 year after surgery, and postoperative inflammatory responses
measured by white blood cell count, neutrophil percentage, and C-reactive protein.
Immune function and inflammatory responses were measured before surgery and 24 and
48hours after surgery.Fifty-nine patients completed the study. In the circumstance
of similar pain control efficacy between the opioid group and the ON-Q group,
postoperative NKCC and interleukin-2 levels did not differ between the 2 groups.
The incidence of postoperative complications and recurrence or metastasis within 1
year after surgery was comparable between the groups. Postoperative inflammatory
responses were also similar between the groups.When compared with ropivacaine wound
infiltration-based analgesia, fentanyl-based analgesia did not further decrease
NKCC or affect short-term cancer recurrence or metastasis. Thus, a fentanyl-based
analgesic regimen and a ropivacaine wound infiltration-based analgesic regimen can
both be used for postoperative pain management in laparoscopic resection of
colorectal cancer.
AN - WOS:000376927000043
AU - Kim, S. Y.
AU - Kim, N. K.
AU - Baik, S. H.
AU - Min, B. S.
AU - Hur, H.
AU - Lee, J.
AU - Noh, H. Y.
AU - Lee, J. H.
AU - Koo, B. N.
C7 - e3602
DA - MAY
DO - 10.1097/MD.0000000000003602
IS - 19
PY - 2016
SN - 0025-7974
1536-5964
ST - Effects of Postoperative Pain Management on Immune Function After
Laparoscopic Resection of Colorectal Cancer: A Randomized Study
T2 - MEDICINE
TI - Effects of Postoperative Pain Management on Immune Function After
Laparoscopic Resection of Colorectal Cancer: A Randomized Study
VL - 95
ID - 6817
ER -
TY - JOUR
AB - Phagocytosis or endocytosis by macrophages is critical to the uptake of fine
particles, including nanoparticles, in order to initiate toxic effects in cells.
Here, our data enhance the understanding of the process of internalization of
silver nanoparticles by macrophages. When macrophages were pre-treated with
inhibitors to phagocytosis, caveolin-mediated endocytosis, or clathrin-mediated
endocytosis, prior to exposure to silver nanoparticles, Interleukin-8 (IL-8)
production was inhibited. Although cell death was not reduced, the inflammatory
response by macrophages was compromised by phagocytosis and endocytosis inhibitors.
© Yonsei University College of Medicine 2012.
AU - Kim, S.
AU - Choi, I. H.
DB - Scopus
DO - 10.3349/ymj.2012.53.3.654
IS - 3
KW - Endocytosis
IL-8
Macrophages
Phagocytosis
Cell Line
Cell Survival
Humans
Interleukin-8
Metal Nanoparticles
Silver
caveolin
chlorpromazine
clathrin
cytochalasin D
interleukin 8
nystatin
silver nanoparticle
article
cell death
cytokine production
drug cytotoxicity
endocytosis
human
human cell
inflammation
macrophage
phagocytosis
M3 - Article
PY - 2012
SP - 654-657
ST - Phagocytosis and endocytosis of silver nanoparticles induce interleukin-8
production in human macrophages
T2 - Yonsei Medical Journal
TI - Phagocytosis and endocytosis of silver nanoparticles induce interleukin-8
production in human macrophages
84859784070&doi=10.3349%2fymj.2012.53.3.654&partnerID=40&md5=801e806d15c1ec8fa12cbf
b9b915fc21
VL - 53
ID - 5718
ER -
TY - JOUR
AB - Objectives: The AH26 of epoxy resin-based sealer is used widely owing to its
excellent physical characteristics but it induces oxidative stress and cytotoxicity
at the periapical tissues. AH26 exhibited cytotoxicity towards MC-3T3-E1 cells,
which resulted in mitochondria-mediated apoptosis. Peroxisome proliferator-
activated receptor (PPARγ) has an anti-inflammatory effect in several tissue and
cells, but its action of AH26-related inflammation is not completely understood.
The aim of this study is to investigate the anti-inflammatory and anti-osteoclastic
mechanisms of PPARγ in AH26-induced MC-3T3 E1 cells. Methods: AH26 was prepared
according to the manufacturer's instructions. The 1-day extraction sample, which
was diluted by 30%, was tested in this experiment. Recombinant deficiency
adenoviral PPARγ (Ad/PPARγ) was used to examine PPARγ over-expression in MC-3T3 E1
cells. AH26-induced reactive oxygen species (ROS) formation was analysed using
2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) with fluorescence-activated
cell sorting (FACS), and the expression of receptor activator of nuclear factor-κB
ligand (RANKL) and inflammatory molecules was determined by immunoblotting. The
anti-inflammatory and anti-osteoclastic mechanisms of the PPARγ-involved signal
pathway was examined by immunoblotting. Results: The AH26 elutes induced inducible
nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), RANKL expression and ROS
formation. In addition, the AH26 elutes suppressed the expression of PPARγ.
However, the recovery of PPARγ expression with Ad/PPARγ resulted in the inhibition
of iNOS, COX-2, RANKL and ROS formation despite the AH26 treatment in MC-3T3 E1
cells. The mechanism of PPARγ was confirmed by the blocking of nuclear factor kappa
B (NF-κB) translocation to the nucleus after the suppression of ERK1/2, SAPK/JNK
and AP-1 in AH26-induced MC-3T3 E1 cells. Conclusion: From this result, PPARγ acts
to inhibit bone destruction in AH26-induced bone cells. Therefore, the anti-
inflammatory and anti-osteoclastic character of PPARγ might be applicable for
healing periapical lesions more rapidly or reducing the induction of cellular
inflammation caused by some endodontic sealers. © 2012 Elsevier Ltd.
AU - Kim, T. G.
AU - Lee, Y. H.
AU - Bhattari, G.
AU - Lee, N. H.
AU - Lee, K. W.
AU - Yi, H. K.
AU - Yu, M. K.
DB - Scopus
DO - 10.1016/j.archoralbio.2012.04.015
IS - 1
KW - AH26
Anti-inflammation
MAPKs
NF-κB
PPARγ
RANKL
3T3 Cells
Adenoviridae
Animals
Bismuth
Blotting, Western
Cell Separation
Cyclooxygenase 2
Epoxy Resins
Extracellular Signal-Regulated MAP Kinases
Flow Cytometry
Fluoresceins
Fluorescent Dyes
Genetic Vectors
MAP Kinase Kinase 4
MAP Kinase Signaling System
Mice
NF-kappa B
Osteoblasts
Osteoclasts
PPAR gamma
RANK Ligand
Signal Transduction
Silver
Titanium
Transcription Factor AP-1
Transfection
autacoid
bismuth
cyclooxygenase 2
diacetyldichlorofluorescein
diagnostic agent
epoxy resin
epoxy resin AH 26
epoxy resin AH-26
fluorescein derivative
fluorescent dye
mitogen activated protein kinase kinase 4
Nos2 protein, mouse
osteoclast differentiation factor
Ptgs2 protein, mouse
silver
titanium
Tnfsf11 protein, mouse
Adenovirus
animal
article
cell separation
cell strain 3T3
drug antagonism
drug effect
flow cytometry
gene vector
genetics
metabolism
mouse
osteoblast
osteoclast
signal transduction
Western blotting
M3 - Article
PY - 2013
SP - 28-34
ST - PPARγ inhibits inflammation and RANKL expression in epoxy resin-based sealer-
induced osteoblast precursor cells E1 cells
T2 - Archives of Oral Biology
TI - PPARγ inhibits inflammation and RANKL expression in epoxy resin-based sealer-
induced osteoblast precursor cells E1 cells
84870460411&doi=10.1016%2fj.archoralbio.2012.04.015&partnerID=40&md5=9faa24d5317d83
75d2957283ef1d29ee
VL - 58
ID - 5722
ER -
TY - JOUR
AB - Resistance to tumor necrosis factor-related apoptosis-inducing ligand
(TRAIL)-induced apoptosis has been reported in some cancer cells, including AGS
human gastric adenocarcinoma cells. Hizikia fusiforme is a commonly used brown
seaweed species in Korea that possesses potent antibacterial, antifungal, and anti-
inflammatory activities. In this study, we demonstrated that treatment with TRAIL
in combination with subtoxic concentrations of ethyl alcohol extract of H.
fusiforme (EAHF) sensitized TRAIL-resistant AGS cells to TRAIL-mediated apoptosis.
Combined treatment with EAHF and TRAIL increased chromatin condensation, DNA
fragmentation, and sub-G1-phase DNA content. The restored sensitivity to TRAIL-
induced apoptosis appeared to be correlated with the modulation of Bcl-2 family
proteins and activation of caspases, which resulted in the cleavage of poly(ADP-
ribose) polymerase. Taken together, the use of EAHF in combination with TRAIL may
be an effective and selective anticancer strategy via suppressing the resistance to
TRAIL-induced apoptosis in some tumor cell lines, including AGS cells.
AN - WOS:000269569500012
AU - Kim, T. Y.
AU - Jin, C. Y.
AU - Kim, G. Y.
AU - Choi, I. W.
AU - Jeong, Y. K.
AU - Nam, T. J.
AU - Kim, S. K.
AU - Choi, Y. H.
DA - AUG
DO - 10.1089/jmf.2008.1114
IS - 4
PY - 2009
SN - 1096-620X
1557-7600
SP - 782-787
ST - Ethyl Alcohol Extracts of Hizikia fusiforme Sensitize AGS Human Gastric
Adenocarcinoma Cells to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-
Mediated Apoptosis
T2 - JOURNAL OF MEDICINAL FOOD
TI - Ethyl Alcohol Extracts of Hizikia fusiforme Sensitize AGS Human Gastric
Adenocarcinoma Cells to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-
Mediated Apoptosis
VL - 12
ID - 6243
ER -
TY - JOUR
AB - Nano-silver (Ag) with antimicrobial activity is by far the most
commercialized nano-compound. The hazards associated with human exposure to
nanosized-silver should be investigated to facilitate the risk assessment process.
Recent studies have shown that inflammatory, oxidative, genotoxic, and cytotoxic
consequences are associated with silver particulate exposure, and are inherently
linked. In the present study, the cytotoxicity and genotoxicity of nano-silver were
investigated using the dye exclusion assay, the comet assay, and the mouse lymphoma
thymidine kinase (tk+/-) gene mutation assay (MLA). IC20 values of nano-silver in
L5178Y cells were determined the concentration of 3,769.53 μg/mL and 1,796.88 μg/mL
with and without S-9, respectively. And in BEAS-2B cell, IC20 values were
calculated to 1,171.88 μg/mL and 761.72 μg/mL with and without S-9, respectively.
From these results, nano-silver was more cytotoxic in absence of S-9 metabolic
activation system and at the BEAS-2B cells. In the comet assay, L5178Y cells and
BEAS-2B cells were treated with nano-silver which significantly increased >2-fold
tail moment with and without S-9. However, the mutant frequencies in the nano-
silver treated L5178Y cells were slightly increased but not significant compared to
the vehicle controls with and without S-9. The results of this study indicate that
nano-silver can cause primary DNA damage and cytotoxicity but not mutagenicity in
cultured mammalian cells. © 2010 The Korean Society of Toxicogenomics and
Toxicoproteomics and Springer Netherlands.
AU - Kim, Y. J.
AU - Yang, S. I.
AU - Ryu, J. C.
DB - Scopus
DO - 10.1007/s13273-010-0018-1
IS - 2
KW - BEAS-2B cell
Comet assay
Cytotoxicity
Gene mutation assay (MLA)
L5178Y cell
Nano-silver
M3 - Article
PY - 2010
SP - 119-125
ST - Cytotoxicity and genotoxicity of nano-silver in mammalian cell lines
T2 - Molecular and Cellular Toxicology
TI - Cytotoxicity and genotoxicity of nano-silver in mammalian cell lines
77956565849&doi=10.1007%2fs13273-010-0018-
1&partnerID=40&md5=610ed7548509a52a3952125eef03c1bf
VL - 6
ID - 5701
ER -
TY - JOUR
AB - In the present study, aqueous leaf extract of Strobilanthes cordifolia
J.R.I.Wood was combined with silver nitrate to synthesis silver nanoparticles
(AgNPs). The AgNPs were characterized using visible spectroscopy (UV), X-ray
diffraction (XRD), Fourier transform infrared spectrophotometer (FTIR), scanning
electron microscope (SEM), energy dispersive X-ray (EDaX), particle size analysis,
and transmission electron microscope (TEM). The UV spectrum absorption peak
occurred at 438 nm. The FTIR analysis of the AgNPs indicated the presence of
functional groups such as aldehyde, alkenes, and carboxylic acids. The crystalline
structure of AgNPs was confirmed by XRD. The AgNPs have a spherical shape according
to SEM. The AgNPs components composition was confirmed by EDaX. The particle size
distribution of AgNPs is monodispersion in the range at 42.54 nm. TEM demonstrated
the AgNPs size to be between 11.35 and 34.90 nm. The AgNPs exhibited good
antibacterial property against Escherichia coli and Staphylococcus aureus. The
antioxidant activity of the AgNPs was represented by increased DPPH, ABTS, and H2O2
activities.The antidiabetic activity of the AgNPs was indicated by the inhibition
of α-amylase and α-glycosidase and anti-inflammatory highest albumin denaturation
and HRBC membrane stabilization properties. Further, the AgNPs also significantly
inhibited the MCF-7 cell lines. These results clearly suggest that the synthesized
AgNPs using S. cordifolia leaves could have several potential biomedical
applications. © 2022 International Union of Biochemistry and Molecular Biology,
Inc.
AU - Kirubakaran, D.
AU - Selvam, K.
AU - Prakash, P.
AU - Manimegalai, P.
AU - Shivakumar, M. S.
AU - SenthilNathan, S.
DB - Scopus
DO - 10.1002/bab.2406
IS - 2
antibacterial
antidiabetic
antioxidant
antiproliferative
characterization
green synthesis
Metal Nanoparticles
Plant Extracts
Silver
Wood
X-Ray Diffraction
Cell culture
Escherichia coli
Metal nanoparticles
Particle size
Silver compounds
Ultraviolet spectroscopy
X ray diffraction
albumin
aldehyde
alkene
alpha glycosidase
amylase
ascorbic acid
carboxylic acid
glycosidase
intestine enzyme
silver nanoparticle
silver nitrate
unclassified drug
antiinfective agent
metal nanoparticle
plant extract
silver
Anti-inflammatories
Anti-proliferative
Antibacterials
Antidiabetic
Characterization
Fourier transform infrared spectrophotometers
Green synthesis
Scanning electrons
Visible spectroscopy
X- ray diffractions
Acanthaceae
Article
biological activity
biosynthesis
chemical analysis
controlled study
crystal structure
cytotoxicity assay
denaturation
drug absorption
drug synthesis
human
human cell
hydrogen peroxide scavenging assay
MCF-7 cell line
nanotechnology
optical spectroscopy
particle size
plant leaf
Strobilanthes cordifolia
wood
zeta potential
chemistry
Antioxidants
M3 - Article
PY - 2023
SP - 870-884
ST - Preparation and characterization of biogenic silver nanoparticles using
Strobilanthes cordifolia (Vahl) J.R.I. Wood leaves and its biological applications
T2 - Biotechnology and Applied Biochemistry
TI - Preparation and characterization of biogenic silver nanoparticles using
Strobilanthes cordifolia (Vahl) J.R.I. Wood leaves and its biological applications
85138739928&doi=10.1002%2fbab.2406&partnerID=40&md5=9bb28a3022caf3efd884d789ec3a37f
8
VL - 70
ID - 5055
ER -
TY - JOUR
AB - Silver oxide nanoparticles (AgO-NPs) antioxidant, anti-cancer, anti-
microbial, and tissue repair properties. Gouty arthritis is the inflammation of
tissues and joints caused by the deposition of monosodium urate crystals. In this
experiment, we investigated the anti-hyperuricemic effectiveness of different
concentrations of AgO-NPs in mice. The present study aimed to investigate the
effect of administration of AgO-NPs in monosodium urate (MSU)-induced gouty mice
for the very first time. Monosodium urate (MSU) crystals were administered
intraperitoneal for gout induction, followed by 5, 10, and 20 mu g/mL doses of AgO-
NPs for 2 weeks. The positive control was provided with the commercially available
drug allopurinol to compare the effects of AgO-NPs and allopurinol. The main
purpose of the study was to investigate the effectiveness of the nanoparticles in
comparison with commercially available drugs. AgO-NPs have been shown to improve
the condition of gouty arthritis by reducing significantly (P < 0.001) increased
levels of ALT, AST, and total bilirubin. The total protein estimation results
showed significant improvement at concentration of 20 mu g/mL of AgO-NPs. The lipid
profile results showed that high concentration (20 mu g/mL) of AgO-NPs decrease the
lipid content significantly as compared to control. It was concluded from this
study that the antioxidant, anti-inflammatory, and antilipidemic properties of AgO-
NPs may improve the hyperuricemic condition in gouty arthritis mice.
AN - WOS:000713068700002
AU - Kiyani, M. M.
AU - Moghul, N. B.
AU - Javed, A.
AU - Butt, M. A.
AU - Abbas, H. B.
AU - Rehman, H.
AU - Rajput, T. A.
AU - Bokhari, S. A. I.
C6 - OCT 2021
DA - AUG
DO - 10.1007/s12011-021-02960-3
IS - 8
PY - 2022
SN - 0163-4984
1559-0720
SP - 3677-3687
ST - In Vivo Effects of Orally Administered Different Concentrations of Silver
Oxide Nanoparticles in Hyperuricemic Mice
TI - In Vivo Effects of Orally Administered Different Concentrations of Silver
Oxide Nanoparticles in Hyperuricemic Mice
VL - 200
ID - 6483
ER -
TY - JOUR
AB - The number of patients suffering from chronic wound healing disorders in
Germany alone is estimated to be 2.5-4 million. Therapy related expenses reach 5-8
billion Euros annually. This number is partially caused by costly dressing changes
due to non-standardized approaches and the application of non-evidence-based
topical wound therapies. The purpose of this paper is to elucidate a
straightforward principle for the management of chronic wounds, and to review the
available evidence for the particular therapy options. The T.I.M.E.-principle
(Tissue management, Inflammation and infection control, Moisture balance,
Epithelial [edge] advancement) was chosen as a systematic strategy for wound bed
preparation. Literature was retrieved from the PubMed and Cochrane Library
databases and subjected to selective analysis. Topical wound management should be
carried out according to a standardized principle and should further be
synchronized to the phases of wound healing. Despite the broad implementation of
these products in clinical practice, often no benefit exists in the rate of
healing, when evaluated in meta-analyses or systematic reviews. This insufficient
evidence is additionally limited by varying study designs. In case of non-
superiority, the results suggest to prefer relatively inexpensive wound dressings
over expensive alternatives. Arbitrary endpoints to prove the effectiveness of
wound dressings, contribute to the random use of such therapies. Defining rational
endpoints for future studies as well as the deployment of structured therapy
strategies will be essential for the economical and evidence-based management of
chronic wounds. © The Authors | Journal compilation © Blackwell Verlag GmbH,
Berlin.
AU - Klein, S.
AU - Schreml, S.
AU - Dolderer, J.
AU - Gehmert, S.
AU - Niederbichler, A.
AU - Landthaler, M.
AU - Prantl, L.
DB - Scopus
DO - 10.1111/ddg.12138
IS - 9
Bandages
Combined Modality Therapy
Debridement
Evidence-Based Medicine
Humans
Skin
Wound Healing
alginic acid
antiinfective agent
cadexomer iodine
calcium alginate
elase
iodine
iruxol
iruxolum mono
natural product
octenidine
polyhexanide
povidone iodine
silver
streptodornase plus streptokinase
unclassified drug
angiogenesis
antisepsis
artificial skin
autolytic debridement
bacterial infection
biocompatibility
chronic inflammation
chronic vein insufficiency
chronic wound
clinical effectiveness
clinical practice
Cochrane Library
colony forming unit
compression bandage
compression therapy
debridement
decubitus
Diptera
drug efficacy
drug induced disease
epithelization
evidence based medicine
fibroblast
foam dressing
granulation tissue
hemosiderosis
honey
human
hydrocolloid
hydrogel
hypoxemia
immunopathology
infection control
infection risk
ionizing radiation
keratinocyte
lavage
lipodermatosclerosis
Lucilia sericata
maggot therapy
Manuka honey
marjolin ulcer
mechanical debridement
medical literature
Medline
meta analysis (topic)
metabolic disorder
moisture
neoplasm
pathophysiology
peripheral occlusive artery disease
plastic surgery
purpura
review
skin epithelium
skin ulcer
systematic review (topic)
tissue injury
tissue pressure
topical treatment
wound care
wound dressing
wound healing
wound infection
M3 - Review
PY - 2013
SP - 819-829
ST - Evidenzbasierte topische Therapie chronischer Wunden nach dem T.I.M.E.-
Prinzip
T2 - JDDG - Journal of the German Society of Dermatology
TI - Evidence-based topical management of chronic wounds according to the T.I.M.E.
principle
84883487301&doi=10.1111%2fddg.12138&partnerID=40&md5=b5c17bcedeff9d71714319737c6c50
16
VL - 11
ID - 5681
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are utilized in surgical implants and medical
textiles, thus providing access to the circulation. While research has been
conducted primarily in healthy models, AgNP-induced toxicity evaluations in disease
conditions are critical, as many individuals have preexisting conditions.
Specifically, over 20% of United States adults suffer from metabolic syndrome
(MetS). It was hypothesized that MetS may increase susceptibility to AgNP-mediated
toxicity due to induction of differential inflammation and altered biodistribution.
Mice were injected with 2 mg/kg AgNPs, and organs assessed for inflammatory gene
expression (TNF-α, CXCL1, CXCL2, CCL2, TGF-β, HO-1, IL-4, IL-13), and Ag content.
AgNPs were determined to induce differential inflammation in healthy and MetS mice.
While AgNP exposure increased TNF-α, CXCL1, TGF-β, HO-1, and IL-4 expression within
healthy mouse spleens, MetS-treated animals demonstrated decreased CXCL1, IL-4, and
IL-13 expression. Healthy and MetS mice livers exhibited similar inflammatory
responses to one another. AgNPs localized primarily to the liver and spleen,
although Ag was present in all examined organs. In organs of minor AgNP deposition,
such as kidney, gene expression was variable. Induction of inflammatory genes did
not correspond with biodistribution, suggesting disease-related variations in AgNP-
mediated adverse responses. These findings indicate that disease may influence
inflammation and biodistribution, impacting AgNP clinical applications. © 2020, ©
2020 Taylor & Francis.
AU - Kobos, L.
AU - Alqahtani, S.
AU - Xia, L.
AU - Coltellino, V.
AU - Kishman, R.
AU - McIlrath, D.
AU - Perez-Torres, C.
AU - Shannahan, J.
DB - Scopus
DO - 10.1080/15287394.2020.1748779
IS - 7
KW - nanomedicine
nanotoxicity
Obesity
Animals
Inflammation
Male
Metabolic Syndrome
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Oxidative Stress
Silver
Tissue Distribution
cholesterol
CXCL1 chemokine
CXCL2 chemokine
heme oxygenase 1
hemoglobin A1c
high density lipoprotein
insulin
interleukin 13
interleukin 4
low density lipoprotein
silver nanoparticle
very low density lipoprotein
metal nanoparticle
silver
animal experiment
animal model
animal tissue
aorta
Article
body weight gain
brain
comparative study
controlled study
gene expression
heart
inflammation
intestine
kidney
limit of detection
liver
lung
male
mouse
mouse model
nonhuman
oxidative stress
priority journal
silver nanoparticle induced inflammation
spleen
upregulation
animal
C57BL mouse
disease model
genetics
immunology
tissue distribution
M3 - Article
PY - 2020
SP - 249-268
ST - Comparison of silver nanoparticle-induced inflammatory responses between
healthy and metabolic syndrome mouse models
T2 - Journal of Toxicology and Environmental Health - Part A: Current Issues
TI - Comparison of silver nanoparticle-induced inflammatory responses between
healthy and metabolic syndrome mouse models
85083585241&doi=10.1080%2f15287394.2020.1748779&partnerID=40&md5=68a5e55a632ccf6b1a
a25c23fed05ca7
VL - 83
ID - 5278
ER -
TY - JOUR
AB - In most conventional in vitro toxicological assays, the response of a
complete cell population is averaged, and therefore, single-cell responses are not
detectable. Such averaging might result in misinterpretations when only individual
cells within a population respond to a certain stimulus. Therefore, there is a need
for non-invasive in vitro systems to verify the toxicity of nanoscale materials. In
the present study, a micro-sized cell culture chamber with a silicon nitride
membrane (0.16 mm 2) was produced for cell cultivation and the detection of
specific cell responses. The biocompatibility of the microcavity chip (MCC) was
verified by studying adipogenic and neuronal differentiation. Thereafter, the
suitability of the MCC to study the effects of nanoparticles on a small cell
population was determined by using a green fluorescence protein-based reporter cell
line. Interleukin-8 promoter (pIL8) induction, a marker of an inflammatory
response, was used to monitor immune activation. The validation of the MCC-based
method was performed using well-characterized gold and silver nanoparticles. The
sensitivity of the new method was verified comparing the quantified pIL8 activation
via MCC-based and standard techniques. The results proved the biocompatibility and
the sensitivity of the microculture chamber, as well as a high optical quality due
to the properties of Si 3N 4. The MCC-based method is suited for threshold- and
time-dependent analysis of nanoparticle-induced IL8 promoter activity. This novel
system can give dynamic information at the level of adherent single cells of a
small cell population and presents a new non-invasive in vitro test method to
assess the toxicity of nanomaterials and other compounds. © 2011 Kohl et al.
AU - Kohl, Y.
AU - Oostingh, G. J.
AU - Sossalla, A.
AU - Duschl, A.
AU - von Briesen, H.
AU - Thielecke, H.
C7 - 505
DB - Scopus
DO - 10.1186/1556-276X-6-505
KW - Inflammation
Micro-sized cell culture chamber
Nanoparticles
Biocompatibility
Cell proliferation
Cells
Silicon nitride
Silver
Toxicity
Adipogenic
Cell cultivation
Cell populations
Cell response
Culture chambers
Dynamic information
Green fluorescence
Immune activation
In-vitro
In-vitro tests
Individual cells
Interleukin-8
Nano-scale materials
Neuronal differentiation
Non-invasive
Optical qualities
Promoter activities
Reporter cell line
Silicon nitride membrane
Single cells
Time-dependent analysis
Cell culture
M3 - Article
PY - 2011
SP - 1-14
ST - Biocompatible micro-sized cell culture chamber for the detection of
nanoparticle-induced IL8 promoter activity on a small cell population
T2 - Nanoscale Research Letters
TI - Biocompatible micro-sized cell culture chamber for the detection of
nanoparticle-induced IL8 promoter activity on a small cell population
84856058917&doi=10.1186%2f1556-276X-6-
505&partnerID=40&md5=927b2d0ad2ebd102f728ad84719ef0da
VL - 6
ID - 5750
ER -
TY - JOUR
AB - The plant extracts are known for their anti-inflammatory, antifungal,
antiviral and antibacterial properties. The use of plant extracts in the
preparation of bio-materials increases their biological application. In this
concern, herein reporting an eco-friendly procedure which is also a simple and cost
effective, for the synthesis of Zinc Oxide nanoparticles (ZnONPs) using Saussurea
lappa plant root (rhizome) extract as a fuel. The prepared nanoparticles were
confirmed using various characterization techniques. The Dynamic light scattering
data showed 123.5 nm particle size with -99.9 mv zeta potential which indicates
excellent stability of the particles. The peak at 541 cm(-1) in the IR spectrum is
assigned to the stretching frequency of the zinc-binding to oxygen. The X-ray
diffraction peaks confirm the close association with JCPDS Data Card No: 36-1451.
The FESEM data revealed a hexagonal wurtzite structure with a hexagonal shape of
synthesized ZnO nanoparticles. The antibacterial studies indicate the gram-negative
strains showed better inhibition activity than gram-positive strains. Among Fungal
strains, Aspergillus niger and flavus, Fusarium oxysporum, and Rhizopus oryzae
showed good inhibition activity at higher concentrations. The cytotoxic data
indicates the 5 mu g/mL of the ZnO particles showed cytotoxicity on the CHO cell
line and with IC50 value 3.164 +/- 0.8956 mu g/mL.
AN - WOS:000675884200023
AU - Kolahalam, L. A.
AU - Prasad, K. R. S.
AU - Krishna, P. M.
AU - Supraja, N.
C6 - JUN 2021
C7 - e07265
DA - JUN
DO - 10.1016/j.heliyon.2021.e07265
IS - 6
PY - 2021
SN - 2405-8440
ST - Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles:
synthesis, characterization and its antibacterial, antifungal activities and
cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines
T2 - HELIYON
TI - Saussurea lappa plant rhizome extract-based zinc oxide nanoparticles:
synthesis, characterization and its antibacterial, antifungal activities and
cytotoxic studies against Chinese Hamster Ovary (CHO) cell lines
VL - 7
ID - 6557
ER -
TY - JOUR
AB - Purpose: In order to overcome the inflammatory response to bacterial
infection during wound healing, we have fabricated an antibacterial and anti-
inflammatory wound dressing based on polysaccharide riclin and silver nanoparticles
(AgNPs). Methods: The riclin-AgNPs nanocomposite was developed by borohydride
method and was characterized by UV-Vis, TEM, XRD, Zeta potential, DLS. In vitro, we
assessed the cumulative release, antibacterial activities and cytotoxicity. In
vivo, we examined the wound healing in mice wound infection experiment and
inflammatory mediators using histological observations and gene expression
analysis. Results: The riclin/AgNPs nanocomposite hydrogel exhibited nanosized
orbicular particles with high purity and stability. In vitro, the riclin/AgNPs
showed sustained release of AgNPs, effective suppression in pathogen growth and
negligible toxicity toward mammalian fibroblasts and macrophage cells. In vivo, the
riclin/AgNPs treatment leads to faster and smoother growth of fresh skin with
suppressed expression of inflammatory mediators. Conclusion: The reported Riclin-
AgNPs nanocomposite hydrogel showed both antibacterial and anti-inflammatory
functions, which induce significantly accelerated wound healing, indicating great
potential as a novel attractive wound dressing material.
AN - WOS:000811192300001
AU - Kong, C. C.
AU - Chen, S. J.
AU - Ge, W. H.
AU - Zhao, Y.
AU - Xu, X. D.
AU - Wang, S. M.
AU - Zhang, J. F.
DO - 10.2147/IJN.S366899
PY - 2022
SN - 1178-2013
SP - 2629-2641
ST - Riclin-Capped Silver Nanoparticles as an Antibacterial and Anti-Inflammatory
Wound Dressing
TI - Riclin-Capped Silver Nanoparticles as an Antibacterial and Anti-Inflammatory
Wound Dressing
VL - 17
ID - 5844
ER -
TY - JOUR
AB - Purpose: In order to overcome the inflammatory response to bacterial
infection during wound healing, we have fabricated an antibacterial and anti-
inflammatory wound dressing based on polysaccharide riclin and silver nanoparticles
(AgNPs). Methods: The riclin-AgNPs nanocomposite was developed by borohydride
method and was characterized by UV-Vis, TEM, XRD, Zeta potential, DLS. In vitro, we
assessed the cumulative release, antibacterial activities and cytotoxicity. In
vivo, we examined the wound healing in mice wound infection experiment and
inflammatory mediators using histological observations and gene expression
analysis. Results: The riclin/AgNPs nanocomposite hydrogel exhibited nanosized
orbicular particles with high purity and stability. In vitro, the riclin/AgNPs
showed sustained release of AgNPs, effective suppression in pathogen growth and
negligible toxicity toward mammalian fibroblasts and macrophage cells. In vivo, the
riclin/AgNPs treatment leads to faster and smoother growth of fresh skin with
suppressed expression of inflammatory mediators. Conclusion: The reported Riclin-
AgNPs nanocomposite hydrogel showed both antibacterial and anti-inflammatory
functions, which induce significantly accelerated wound healing, indicating great
potential as a novel attractive wound dressing material. © 2022 Kong et al.
AU - Kong, C.
AU - Chen, S.
AU - Ge, W.
AU - Zhao, Y.
AU - Xu, X.
AU - Wang, S.
AU - Zhang, J.
DB - Scopus
DO - 10.2147/IJN.S366899
KW - nanocomposite hydrogels
riclin
wound healing
Animals
Bandages
Mammals
Metal Nanoparticles
Mice
Nanogels
Silver
interleukin 1beta
interleukin 6
nanohydrogel
silver nanoparticle
unclassified drug
antiinfective agent
autacoid
metal nanoparticle
silver
agar diffusion
angiogenesis
animal cell
animal experiment
animal model
animal tissue
Article
biocompatibility
bioluminescence
cell aggregation
cell proliferation
cell proliferation assay
cell viability
controlled study
crystal structure
cytotoxicity
dispersity
epithelization
Escherichia coli
fibroblast
freeze drying
gene expression
histology
histopathology
hydrogen bond
in vitro study
in vivo study
macrophage
male
mammal
mouse
MTT assay
nanotechnology
nonhuman
osteolysis
particle size
shear stress
skin biopsy
sustained release preparation
tissue regeneration
X ray diffraction
zeta potential
zone of inhibition
animal
bandage
M3 - Article
PY - 2022
SP - 2629-2641
ST - Riclin-Capped Silver Nanoparticles as an Antibacterial and Anti-Inflammatory
Wound Dressing
TI - Riclin-Capped Silver Nanoparticles as an Antibacterial and Anti-Inflammatory
Wound Dressing
85132306960&doi=10.2147%2fIJN.S366899&partnerID=40&md5=3b11e9c2f77952b972cab5732107
8e6e
VL - 17
ID - 5095
ER -
TY - JOUR
AB - Keratin is a cytoskeletal scaffolding protein essential for wound healing and
tissue recovery. The aim of the study was to evaluate the potential role of
insoluble fur keratin-derived powder containing silver nanoparticles (FKDP-AgNP) in
the allogenic full-thickness surgical skin wound model in diabetic mice. The
scanning electron microscopy image evidenced that the keratin surface is covered by
a single layer of silver nanoparticles. Data obtained from dynamic light scattering
and micellar electrokinetic chromatography showed three fractions of silver
nanoparticles with an average diameter of 130, 22.5, and 5 nm. Microbiologic
results revealed that the designed insoluble FKDP-AgNP dressing to some extent
inhibit the growth of Escherichia coli and Staphylococcus aureus. In vitro assays
showed that the FKDP-AgNP dressing did not inhibit fibroblast growth or induce
hemolysis. In vivo studies using a diabetic mice model confirmed biocompatible
properties of the insoluble keratin dressings. FKDP-AgNP significantly accelerated
wound closure and epithelization at Days 5 and 8 (p <.05) when compared with
controls. Histological examination of the inflammatory response documented that
FKDP-AgNP-treated wounds contained predominantly macrophages, whereas their
untreated variants showed mixed cell infiltrates rich in neutrophils. Wound
inflammatory response based on macrophages favors tissue remodeling and healing. In
conclusion, the investigated FKDP-AgNP dressing consisting of an insoluble fraction
of keratin, which is biocompatible, significantly accelerated wound healing in a
diabetic mouse model. © 2019 John Wiley & Sons, Ltd.
AU - Konop, M.
AU - Czuwara, J.
AU - Kłodzińska, E.
AU - Laskowska, A. K.
AU - Sulejczak, D.
AU - Damps, T.
AU - Zielenkiewicz, U.
AU - Brzozowska, I.
AU - Sureda, A.
AU - Kowalkowski, T.
AU - Schwartz, R. A.
AU - Rudnicka, L.
DB - Scopus
DO - 10.1002/term.2998
IS - 2
KW - chronic wounds
diabetic mice
inflammation
keratin wound dressing
skin wound healing
Animals
Bandages
Cell Movement
Cell Proliferation
Cell Survival
Colloids
Cytokines
Escherichia coli
Inflammation
Keratins
Kinetics
Light
Male
Metal Nanoparticles
Mice
Mice, Inbred C57BL
NIH 3T3 Cells
Signal Transduction
Silver
Skin
Wound Healing
Biocompatibility
Cell culture
Keratin
Light scattering
Macrophages
Mammals
Metal nanoparticles
Scaffolds (biology)
Tissue
Tissue regeneration
biomaterial
keratin
silver nanoparticle
antiinfective agent
cytokine
metal nanoparticle
silver
Chronic wounds
Cytoskeletal
Diabetic mice
Keratin wound dressing
Mice models
Scaffolding proteins
Skin wound healing
Wound dressings
Wound healing
animal cell
animal experiment
animal model
animal tissue
Article
bacterial cell
bacterial growth
biocompatibility
brain hemorrhage
C57BL 6 mouse
cell growth
cell infiltration
cell viability
controlled study
cytokine response
dermatitis
diabetes mellitus
electrophoresis
epithelization
growth inhibition
healing rate
histology
in vitro study
in vivo study
macrophage
male
micellar electrokinetic chromatography
mouse
nonhuman
particle size
priority journal
protein analysis
skin injury
surgical wound
tissue regeneration
tissue repair
wound closure
animal
bandage
C57BL mouse
cell motion
cell proliferation
cell survival
chemistry
colloid
drug effect
kinetics
light
metabolism
NIH 3T3 cell line
pathology
signal transduction
skin
wound healing
M3 - Article
PY - 2020
SP - 334-346
ST - Evaluation of keratin biomaterial containing silver nanoparticles as a
potential wound dressing in full-thickness skin wound model in diabetic mice
T2 - Journal of Tissue Engineering and Regenerative Medicine
TI - Evaluation of keratin biomaterial containing silver nanoparticles as a
potential wound dressing in full-thickness skin wound model in diabetic mice
85078609106&doi=10.1002%2fterm.2998&partnerID=40&md5=b1f1cb7b2e5cd14e12dbb953748926
b2
VL - 14
ID - 5283
ER -
TY - JOUR
AB - Aims and Objectives: The study aims at determining pulp response of two high
fluoride releasing materials silver diamine fluoride (SDF) and Type VII glass
ionomer cement (GIC) when used as indirect pulp treatment (IPT) materials.
Materials and Methods: Deep Class V cavities were made on four first premolars
indicated for extraction for orthodontic reasons. SDF, Type VII GIC, and calcium
hydroxide base are given in three premolars, and one is kept control. Premolars
were extracted 6 weeks after the procedure and subjected to histopathological
examination to determine the pulp response. The results were analyzed using Chi-
square test. Results: No inflammatory changes were observed in any of the groups.
Significantly more number of specimens in SDF and Type VII GIC groups showed
tertiary dentin deposition (TDD) when compared to control group. No significant
difference was seen in TDD when intergroup comparison was made. Odontoblasts were
seen as short cuboidal cells with dense basophilic nucleus in SDF and Type VII GIC
group. Conclusion: The study demonstrated TDD inducing ability of SDF and Type VII
GIC and also established the biocompatibility when used as IPT materials.
AU - Korwar, A.
AU - Sharma, S.
AU - Logani, A.
AU - Shah, N.
DB - Scopus
DO - 10.4103/0976-237X.161855
IS - 3
KW - Calcium hydroxide
high fluoride releasing glass ionomer
indirect pulp treatment
pulpal response
silver diamine fluoride
M3 - Article
PY - 2015
SP - 288-292
ST - Pulp response to high fluoride releasing glass ionomer, silver diamine
fluoride, and calcium hydroxide used for indirect pulp treatment: An in-vivo
comparative study
T2 - Contemporary Clinical Dentistry
TI - Pulp response to high fluoride releasing glass ionomer, silver diamine
fluoride, and calcium hydroxide used for indirect pulp treatment: An in-vivo
comparative study
84938495133&doi=10.4103%2f0976-
237X.161855&partnerID=40&md5=294dc8ef3e376663346e998f01bd0c6d
VL - 6
ID - 5579
ER -
TY - JOUR
AB - Introduction. The current direction in medicine is the creation of
immunobiological preparations to increase the effectiveness of specific
immunotherapy and immunoprophylaxis, containing immunomodulators in their
composition. These natural or synthetic substances can have a regulating effect on
the immune system. At present, water-soluble organic-inorganic polymeric materials
with nanoparticles of various chemical substances with bactericidal and
immunomodulating properties can serve as such promising compounds. The aim of the
work is to study the acute toxicity of polymer nanocomposites based on 1-vinil-
1,2,4-triazole copolymer with N-vinylpyrrolidone with silver, gold and selenium
nanoparticles and their effect on the functional state of immune system cells in
vitro. Materials and methods. The study of acute toxicity was performed on outbred
white mice. The activity of superoxide dismutase and glucose-6-phosphate
dehydrogenase were studied in guinea pig peritoneal macrophages. The study of
spontaneous and nanocomposite-induced production of pro- (interferon gamma and
tumor necrosis factor alpha) and anti-inflammatory (interleukin-4) cytokines by
blood cells was carried out using clinical material obtained from volunteers using
the ELISA method. Results. It has been established that nanocomposites with silver
and gold nanoparticles do not cause the death of white mice, their temperature
increase and body weight decrease. The average lethal dose for a nanocomposite with
selenium nanoparticles was determined as 1 gram per 1 kilogram of animal mass. It
was shown that the tested nanocomposites have a stimulating effect on the
production of cytokines by human blood cells in vitro. It was established that a
nanocomposite with selenium nanoparticles increases the activity of superoxide
dismutase and glucose-6-phosphate dehydrogenase. A comparative analysis of their
actions with the actions of commercial preparations of biological origin, with
immunomodulatory properties. Conclusion. The data obtained allow us to substantiate
the need for further research on the effects of nanocomposites based on 1-vinyl-
1,2,4-triazole copolymer with N-vinylpyrrolidone with silver, gold and selenium
nanoparticles on the macroorganism in both in vitro and in vivo conditions. © 2021
Acta Biomedica Scientifica. All rights reserved.
AU - Korytov, K. M.
AU - Dubrovina, V. I.
AU - Vityazeva, S. A.
AU - Pyatidesyatnikova, A. B.
AU - Voitkova, V. V.
AU - Prozorova, G. F.
AU - Pozdnyakov, A. S.
AU - Ivanova, A. A.
AU - Balakhonov, S. V.
DB - Scopus
DO - 10.29413/ABS.2019-4.3.13
IS - 3
KW - Acute toxicity
Blood cell
Cytokine
Glucose-6-phosphate dehydrogenase
Macrophage
Nanocomposite
Nanoparticle
Superoxide dismutase
M3 - Article
PY - 2019
SP - 102-109
ST - Assessment of toxic and immunoadjuvant properties of nanocomposites
T2 - Acta Biomedica Scientifica
TI - Assessment of toxic and immunoadjuvant properties of nanocomposites
85101560225&doi=10.29413%2fABS.2019-
4.3.13&partnerID=40&md5=201510333f9a21319c2ca0ec45a8962a
VL - 4
ID - 5361
ER -
TY - JOUR
AB - Eosinophils, macrophages and other leucocytes invade the uterine endometrium
during oestrus and play a role in the tissue remodeling and immune responses that
occur prior to implantation of the fertilized ovum. Adenosine 5'-triphosphate (ATP)
and its metabolites influence uterine function via ATP receptors. In this study, we
investigated the presence and localisation of the P2X(7) nucleotide receptor in the
cells that infiltrate the uterine endometrium of adult female rats during oestrus
at the electron microscope level, using gold-silver pre-embedding
immunocytochemical techniques. P2X(7) receptor expression was found in the
cytoplasm and the cell membrane of eosinophils, macrophages and fibroblasts in the
endometrium during oestrus. These results suggest that ATP-mediated responses may
be important in uteri ne preparation and remodeling before implantation and that
this may involve several types of cells. In particular, the presence of P2X(7)
receptors on endometrial stromal cells may indicate their involvement in apoptosis
and immune and inflammatory responses. (C) 2005 Elsevier Ireland Ltd. All rights
reserved.
AN - WOS:000231299800003
AU - Koshi, R.
AU - Coutinho-Silva, R.
AU - Cascabulho, C. M.
AU - Henrique-Pons, A.
AU - Knight, G. E.
AU - Loesch, A.
AU - Burnstock, G.
DA - AUG
DO - 10.1016/j.jri.2005.04.006
IS - 2
PY - 2005
SN - 0165-0378
SP - 127-140
ST - Presence of the P2X(7) purinergic receptor on immune cells that invade the
rat endometrium during oestrus
T2 - JOURNAL OF REPRODUCTIVE IMMUNOLOGY
TI - Presence of the P2X(7) purinergic receptor on immune cells that invade the
rat endometrium during oestrus
VL - 66
ID - 6524
ER -
TY - JOUR
AB - Eosinophils, macrophages and other leucocytes invade the uterine endometrium
during oestrus and play a role in the tissue remodeling and immune responses that
occur prior to implantation of the fertilized ovum. Adenosine 5′-triphosphate (ATP)
and its metabolites influence uterine function via ATP receptors. In this study, we
investigated the presence and localisation of the P2X7 nucleotide receptor in the
cells that infiltrate the uterine endometrium of adult female rats during oestrus
at the electron microscope level, using gold-silver pre-embedding
immunocytochemical techniques. P2X 7 receptor expression was found in the cytoplasm
and the cell membrane of eosinophils, macrophages and fibroblasts in the
endometrium during oestrus. These results suggest that ATP-mediated responses may
be important in uterine preparation and remodeling before implantation and that
this may involve several types of cells. In particular, the presence of P2X7
receptors on endometrial stromal cells may indicate their involvement in apoptosis
and immune and inflammatory responses. © 2005 Elsevier Ireland Ltd. All rights
reserved.
AU - Koshi, R.
AU - Coutinho-Silva, R.
AU - Cascabulho, C. M.
AU - Henrique-Pons, A.
AU - Knight, G. E.
AU - Loesch, A.
AU - Burnstock, G.
DB - Scopus
DO - 10.1016/j.jri.2005.04.006
IS - 2
KW - Implantation
Oestrus
Purinergic receptor
Signal transducer
Uterus
Animals
Apoptosis
Endometrium
Eosinophils
Estrus
Female
Permeability
Rats
Receptors, Purinergic P2
Spleen
gold
purine P2X7 receptor
silver
animal cell
animal tissue
apoptosis
article
cell invasion
cell membrane
cell type
controlled study
cytoplasm
electron microscopy
endometrium
eosinophil
estrus
female
fibroblast
immune response
immunocytochemistry
inflammation
macrophage
nidation
nonhuman
priority journal
protein expression
protein function
rat
stroma cell
tissue repair
M3 - Article
PY - 2005
SP - 127-140
ST - Presence of the P2X7 purinergic receptor on immune cells that invade the rat
endometrium during oestrus
T2 - Journal of Reproductive Immunology
TI - Presence of the P2X7 purinergic receptor on immune cells that invade the rat
endometrium during oestrus
23044512109&doi=10.1016%2fj.jri.2005.04.006&partnerID=40&md5=887922096b69ad43da24e7
6b353f8f68
VL - 66
ID - 5833
ER -
TY - JOUR
AB - Purpose Previously, the family of S 100A proteins has been found to be
associated with inflammation and myelopoiesis and to be able to induce or support
myeloproliferation during chronic inflammation. Here, we studied the inflammatory
myeloid-related proteins Si 00A4, S 100A8, S 100A9 and S100Al2 in
myeloproliferative neoplasms (MPNs) in order to assess the involvement of chronic
inflammation in the pathogenesis of MPN. Methods We analyzed the S100A4, S100A8,
S100A9 and S100Al2 mRNA and protein levels in the bone marrow and circulation of
140 patients with MPN and 15 healthy controls using Western blotting, microarray-
based mRNA expression profiling and ELISA assays, respectively. In addition we
performed functional studies on the proliferation-related AKT and ERK1/2 signaling
pathways in MPN-derived granulocytes using Western blotting and proteomic analyses.
Results We found that the S100A mRNA levels were increased in MPN patient-derived
circulatory CD34(+ )cells, and that their protein expression levels were also
augmented in their granulocytes and bone marrow stroma cells, depending on the
JAK2V617F mutation allele burden. We also found that calreticulin (CALR) mutations
were related to reduced S100A8 plasma levels in primary myelofibrosis (PMF). The
S100A8 plasma levels were found to be increased in MPN, the S100A9 plasma levels in
PMF and essential thrombocythemia (ET), and the S100A12 plasma levels in
polycythemia vera (PV). These 5100A plasma levels showed a positive correlation
with the systemic inflammation marker IL-8, as well as with the numbers of
leukocytes and thrombocytes, depending on the JAK2V617F mutation status.
Additionally, we found that heterodimeric S100A8/9 can inhibit the AKT pathway in
MPN-derived granulocytes mediated by the Toll-like receptor 4 (TLR4), depending on
the CALR mutation status. Conversely, we found that blocking of the receptor for
advanced glycation end products (RAGE) increased the S100A8/9-mediated inhibition
of AKT signaling in the MPN-derived granulocytes. Moreover, we found that
heterodimeric S100A8/9 generally induced TLR4-mediated ERK1/2 dephosphorylation
proportionally to the JAK2V617F mutation allele burden. TLR4/RAGE blocking
prevented the S100A8/9-mediated inhibition of ERK1/2 phosphorylation in PV.
Conclusions From our data we conclude that the 5100A8 and S100A9 granulocyte and
plasma levels are increased in MPN patients, along with inflammation markers,
depending on their JAK2V617F mutation allele burden. We also found that SIO0A8/9-
mediated inhibition of the proliferation-related AKT and ERK1/2 signaling pathways
can be decreased by CALR mutationdependent TLR4 blocking and increased by RAGE
inhibition in MPN.
AN - WOS:000444812800007
AU - Kovacic, M.
AU - Mitrovic-Ajtic, O.
AU - Beleslin-Cokic, B.
AU - Djikic, D.
AU - Suboticki, T.
AU - Diklic, M.
AU - Lekovic, D.
AU - Gotic, M.
AU - Mossuz, P.
AU - Cokic, V. P.
DA - OCT
DO - 10.1007/s13402-018-0392-6
IS - 5
PY - 2018
SN - 2211-3428
2211-3436
SP - 541-553
ST - TLR4 and RAGE conversely mediate pro-inflammatory S100A8/9-mediated
inhibition of proliferation-linked signaling in myeloproliferative neoplasms
T2 - CELLULAR ONCOLOGY
TI - TLR4 and RAGE conversely mediate pro-inflammatory S100A8/9-mediated
inhibition of proliferation-linked signaling in myeloproliferative neoplasms
VL - 41
ID - 6736
ER -
TY - JOUR
AB - Complement-associated factors are implicated in pathogen presentation,
neurodegeneration, and microglia resolution of tissue injury. To characterize
complement activation with microglial clearance of degenerating mossy fiber
boutons, hippocampal dentate granule neurons were ablated in CD-1 mice with
trimethyltin (TMT; 2.2 mg/kg, i.p.). Neuronal apoptosis was accompanied by amoeboid
microglia and elevations in tumor necrosis factor [Tnfa], interleukin 1β [Il1b],
and Il6 mRNA and C1q protein. Inos mRNA levels were unaltered. Silver degeneration
and synaptophysin staining indicated loss of synaptic innervation to CA3 pyramidal
neurons. Reactive microglia with thickened bushy morphology showed co-localization
of synaptophysin+ fragments. The initial response at 2 days post-TMT included
transient elevations in Tnfa, Il1b, Il6, and Inos mRNA levels. A concurrent
increase at 2 days was observed in arginase-1 [Arg1], Il10, transforming growth
factor β1 [Tgfb1], and chitinase 3 like-3 [Ym1] mRNA levels. At 2 days, C1q protein
was evident in the CA3 with elevated C1qa, C1qb, C3, Cr3a, and Cr3b mRNA levels.
mRNA levels remained elevated at 5 days, returning to control by 14 days,
corresponding to silver degeneration. mRNA levels for pentraxin3 (Ptx3) were
elevated on day 2 and Ptx1 was not altered. Our data suggest an association between
microglia reactivity, the induction of anti-inflammatory genes concurrent with pro-
inflammatory genes and the expression of complement-associated factors with the
degeneration of synapses following apoptotic neuronal loss. © 2016, Springer
Science+Business Media New York (outside the USA).
AU - Kraft, A. D.
AU - McPherson, C. A.
AU - Harry, G. J.
DB - Scopus
DO - 10.1007/s12640-016-9606-8
IS - 1
KW - C1q
Interleukin 1
M1/M2 polarization
Microglia
Synapse stripping
Tumor necrosis factor
Animals
Apoptosis
Arginase
beta-N-Acetylhexosaminidases
C-Reactive Protein
Complement System Proteins
Dentate Gyrus
Interleukin-10
Interleukin-1beta
Interleukin-6
Lectins
Male
Mice
Mossy Fibers, Hippocampal
Nerve Degeneration
Paired Box Transcription Factors
Synapses
Synaptophysin
Transforming Growth Factor beta
Trimethyltin Compounds
arginase 1
chitinase
chitinase 3 like 3
complement component C1q
complement component C3
complement factor C1qa
complement factor C1qb
complement factor Cr3a
complement factor Cr3b
interleukin 10
interleukin 1beta
interleukin 6
messenger RNA
pentraxin
pentraxin 1
pentraxin 3
STAT3 protein
synaptophysin
trimethyltin
unclassified drug
Arg1 protein, mouse
arginase
autacoid
C reactive protein
Chi3l3 protein, mouse
complement
homeobox protein PITX1
lectin
nerve protein
neuronal pentraxin
Nos2 protein, mouse
paired box transcription factor
animal experiment
animal tissue
apoptosis
Article
controlled study
granule cell
hippocampal CA3 region
hippocampal mossy fiber
male
microglia
mouse
nonhuman
priority journal
pyramidal nerve cell
animal
dentate gyrus
drug effects
metabolism
nerve degeneration
pathology
synapse
M3 - Article
PY - 2016
SP - 53-66
ST - Association Between Microglia, Inflammatory Factors, and Complement with Loss
of Hippocampal Mossy Fiber Synapses Induced by Trimethyltin
TI - Association Between Microglia, Inflammatory Factors, and Complement with Loss
of Hippocampal Mossy Fiber Synapses Induced by Trimethyltin
84958752887&doi=10.1007%2fs12640-016-9606-
8&partnerID=40&md5=6dd5a24188c7b1b78246ba85b04cd450
VL - 30
ID - 5484
ER -
TY - JOUR
AB - Silver nanoparticles have been used in a range of applications and although
they are already employed in medicine, there are new, promising possibilities for
their utilization. We investigated the potential of silver nanoparticles obtained
with the use of blackcurrant extract in vitro in the LPS-stimulated RAW264.7
macrophages and in vivo in the murine DSS-induced colitis model. The examined
formulations contained particles of 95 nm (Ag95) and 213 nm (Ag213) diameter. In
vitro, both formulations inhibited nitric oxide (NO) release. In vivo, the
preparations alleviated colitis as evidenced by a decreased macroscopic score and
myeloperoxidase activity (indicative of neutrophil infiltration). In both cases,
the nanoparticles of larger diameter showed better anti-inflammatory properties.
Although further tests are required, our results indicate a plausible new use of
silver nanoparticles in inflammatory bowel diseases. © 2020 Elsevier B.V.
AU - Krajewska, J. B.
AU - Długosz, O.
AU - Sałaga, M.
AU - Banach, M.
AU - Fichna, J.
C7 - 119549
DB - Scopus
DO - 10.1016/j.ijpharm.2020.119549
KW - Blackcurrant extract
Colitis
Inflammatory bowel diseases
RAW264.7 macrophages
Animals
Cell Survival
Lipopolysaccharides
Male
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Particle Size
Peroxidase
Plant Extracts
RAW 264.7 Cells
Ribes
Silver
Technology, Pharmaceutical
ascorbic acid
black currant extract
budesonide
myeloperoxidase
nitric oxide
silver nanoparticle
lipopolysaccharide
metal nanoparticle
peroxidase
plant extract
silver
animal cell
animal experiment
animal model
Article
controlled study
cytotoxicity
dextran sulfate sodium-induced colitis
enzyme activity
in vitro study
in vivo study
intestine
macrophage
male
mouse
neutrophil
nonhuman
priority journal
RAW 264.7 cell line
animal
Bagg albino mouse
cell survival
chemistry
colitis
disease model
metabolism
particle size
pharmaceutics
procedures
M3 - Article
PY - 2020
ST - Silver nanoparticles based on blackcurrant extract show potent anti-
inflammatory effect in vitro and in DSS-induced colitis in mice
TI - Silver nanoparticles based on blackcurrant extract show potent anti-
inflammatory effect in vitro and in DSS-induced colitis in mice
85086734120&doi=10.1016%2fj.ijpharm.2020.119549&partnerID=40&md5=d7a9cc2197ae2d8373
ac02ac7cceeabe
VL - 585
ID - 5276
ER -
TY - JOUR
AB - The study was designed to examine the effects of silver AgNPs, 20 nm) and
titanium dioxide (Aeroxide® P25 TiO2NPs, 21 nm) nanoparticles on brain oxidative
stress parameters, its antioxidant potential and brain renin-angiotensin system
(RAS) in vivo. The analysis was performed 28 days after single dose injection of
TiO2NPs and AgNPs (10 or 5 mg/kg body weight, respectively). The AgNPs, but not
TiO2NPs, administration resulted in decreased lipid and cholesterol peroxidation.
Antioxidant enzymes gene expression and/or activity were changed differently for
TiO2NPs and AgNPs group. The TiO2NPs decreased aromatase gene expression, and
glutathione peroxidase and reductase activities. In AgNPs group the sodium
dismutase 1 and glutathione reductase mRNA levels were decreased as opposed to
their activities. Both NPs altered the expression of brain RAS genes
(angiotensinogen, renin, angiotensin I converting enzyme 1 and 2), but only TiO2NPs
caused similar changes on protein level. The expression of amyloid beta precursor
protein gene was not altered by any kind of injected NPs. The TiO2NPs were more
potent modulator of gene expression in the brain than AgNPs, despite the two times
lower dosage. These results suggest that AgNPs and TiO2NPs exposure may modulate
the brain function, but with different strength. © 2015 Elsevier Ltd.
AU - Krawczyńska, A.
AU - Lankoff, A.
AU - Herman, A. P.
AU - Oczkowski, M.
AU - Królikowski, T.
AU - Wilczak, J.
AU - Wojewódzka, M.
AU - Kruszewski, M.
DB - Scopus
DO - 10.1016/j.fct.2015.08.005
KW - Antioxidant enzymes
Brain
Inflammation
Nanoparticles
Oxidative stress
Renin-angiotensin system
Animals
Injections, Intravenous
Lipid Peroxidation
Male
Metal Nanoparticles
Neuritis
Neurons
Oxidative Stress
Oxidoreductases
Particle Size
Rats, Wistar
Renin-Angiotensin System
Silver
Surface Properties
Titanium
Toxicity Tests
amyloid beta protein
angiotensinogen
aromatase
messenger RNA
oxidoreductase
renin
silver nanoparticle
sodium dismutase 1
unclassified drug
metal nanoparticle
nerve protein
silver
titanium
titanium dioxide
amyloid beta precursor protein gene
angiotensin I converting enzyme 1 gene
angiotensin I converting enzyme 2 gene
angiotensinogen gene
animal experiment
animal tissue
aromatase gene
Article
brain
controlled study
enzyme activity
genetic variability
in vivo study
inflammation
lipid peroxidation
male
neuromodulation
nonhuman
oncogene ras
oxidative stress
particle size
rat
renin angiotensin aldosterone system
renin gene
animal
antagonists and inhibitors
chemically induced
chemistry
comparative study
drug effects
enzymology
genetics
immunology
metabolism
nerve cell
neuritis
surface property
toxicity testing
Wistar rat
M3 - Article
PY - 2015
SP - 96-105
ST - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
response and renin-angiotensin system in brain
TI - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
84958604797&doi=10.1016%2fj.fct.2015.08.005&partnerID=40&md5=fa2111e098521d7649ee63
a7cb0a5f0b
VL - 85
ID - 5573
ER -
TY - JOUR
AB - The study was designed to examine the effects of silver AgNPs, 20 nm) and
titanium dioxide (Aeroxide (R) P25 TiO(2)NPs, 21 nm) nanoparticles on brain
oxidative stress parameters, its antioxidant potential and brain renin-angiotensin
system (RAS) in vivo. The analysis was performed 28 days after single dose
injection of TiO(2)NPs and AgNPs (10 or 5 mg/kg body weight, respectively). The
AgNPs, but not TiO(2)NPs, administration resulted in decreased lipid and
cholesterol peroxidation. Antioxidant enzymes gene expression and/or activity were
changed differently for TiO(2)NPs and AgNPs group. The TiO(2)NPs decreased
aromatase gene expression, and glutathione peroxidase and reductase activities. In
AgNPs group the sodium dismutase 1 and glutathione reductase mRNA levels were
decreased as opposed to their activities. Both NPs altered the expression of brain
RAS genes (angiotensinogen, renin, angiotensin I converting enzyme 1 and 2), but
only TiO(2)NPs caused similar changes on protein level. The expression of amyloid
beta precursor protein gene was not altered by any kind of injected NPs. The
TiO(2)NPs were more potent modulator of gene expression in the brain than AgNPs,
despite the two times lower dosage. These results suggest that AgNPs and TiO(2)NPs
exposure may modulate the brain function, but with different strength. (C) 2015
AN - WOS:000366951300012
AU - Krawczynska, A.
AU - Lankoff, A.
AU - Herman, A. P.
AU - Oczkowski, M.
AU - Krolikowski, T.
AU - Wilczak, J.
AU - Wojewodzka, M.
AU - Kruszewski, M.
DA - NOV
DO - 10.1016/j.fct.2015.08.005
PY - 2015
SN - 0278-6915
1873-6351
SP - 96-105
ST - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
TI - Silver and titanium dioxide nanoparticles alter oxidative/inflammatory
VL - 85
ID - 6523
ER -
TY - JOUR
AB - Endophthalmitis is an inflammatory reaction of intraocular fluids or tissues.
Infectious endophthalmitis is one of the most serious complications of ophthalmic
surgery. Occasionally, infectious endophthalmitis depends on timely diagnosis and
institution of appropriate therapy. Recognition of the different clinical settings
in which endophthalmitis occurs and awareness of the highly variable presentation
it may have facilitate timely diagnosis. Biopsy of intraocular fluid/tissue is the
only method that permits reliable diagnosis and treatment. The different presenting
clinical settings, a rational approach to diagnosis (i.e., when, what, and how to
biopsy), and the treatment of infectious endophthalmitis are reviewed.
AU - Kresloff, M. S.
AU - Castellarin, A. A.
AU - Zarbin, M. A.
DB - Scopus
DO - 10.1016/S0039-6257(98)00036-8
IS - 3
KW - Acute postoperative endophthalmitis
Antibiotics
Chronic postoperative endophthalmitis
Endogenousendophthalmitis
Inflammation
Phacoanaphylactic endophthalmitis
Phacolytic glaucoma
Posttrabeculectomy endophthalmitis
Posttraumatic endophthalmitis
Sympathetic ophthalmia
Vitrectomy
Diagnostic Techniques, Ophthalmological
Endophthalmitis
Eye Infections
Humans
Ophthalmologic Surgical Procedures
Risk Factors
amikacin
aminoglycoside antibiotic agent
amphotericin b
antibiotic agent
bacitracin
beta lactam antibiotic
cefazolin
ceftazidime
cephalosporin derivative
chloramphenicol
cidofovir
ciprofloxacin
dexamethasone
gentamicin
heparin
hexachlorophene
low molecular weight heparin
metipranolol
neomycin
neosporin
ofloxacin
polysporin
povidone iodine
prednisone
rifabutin
silver protein
unindexed drug
vancomycin
antibiotic prophylaxis
bacterium culture
biopsy
clinical feature
clinical trial
drug hypersensitivity
endophthalmitis
eye photography
eye surgery
eye toxicity
fungus culture
human
infection
intravitreal drug administration
meta analysis
nonhuman
priority journal
review
risk factor
slit lamp
subconjunctival drug administration
trabeculectomy
uveitis
vitrectomy
M3 - Review
PY - 1998
SP - 193-224
ST - Endophthalmitis
T2 - Survey of Ophthalmology
TI - Endophthalmitis
0031737190&doi=10.1016%2fS0039-6257%2898%2900036-
8&partnerID=40&md5=feee3f2bb9fe1a9ae366e18084dd8f36
VL - 43
ID - 5793
ER -
TY - JOUR
AB - Background There is a steadily increasing quantity of silver nanoparticles
(AgNP) produced for numerous industrial, medicinal and private purposes, leading to
an increased risk of inhalation exposure for both professionals and consumers.
Particle inhalation can result in inflammatory and allergic responses, and there
are concerns about other negative health effects from either acute or chronic low-
dose exposure. Results To study the fate of inhaled AgNP, healthy adult rats were
exposed to 11/2-hour intra-tracheal inhalations of pristine Ag-105-radiolabeled, 20
nm AgNP aerosols (with mean doses across all rats of each exposure group of
deposited NP-mass and NP-number being 13.5 +/- 3.6 mu g, 7.9 +/- 3.2 center dot
10(11), respectively). At five time-points (0.75 h, 4 h, 24 h, 7d, 28d) post-
exposure (p.e.), a complete balance of the [Ag-105]AgNP fate and its degradation
products were quantified in organs, tissues, carcass, lavage and body fluids,
including excretions. Rapid dissolution of [Ag-105]Ag-ions from the [Ag-105]AgNP
surface was apparent together with both fast particulate airway clearance and long-
term particulate clearance from the alveolar region to the larynx. The results are
compatible with evidence from the literature that the released [Ag-105]Ag-ions
precipitate rapidly to low-solubility [Ag-105]Ag-salts in the ion-rich epithelial
lining lung fluid (ELF) and blood. Based on the existing literature, the
degradation products rapidly translocate across the air-blood-barrier (ABB) into
the blood and are eliminated via the liver and gall-bladder into the small
intestine for fecal excretion. The pathway of [Ag-105]Ag-salt precipitates was
compatible with auxiliary biokinetics studies at 24 h and 7 days after either
intravenous injection or intratracheal or oral instillation of [Ag-110m]AgNO3
solutions in sentinel groups of rats. However, dissolution of [Ag-105]Ag-ions
appeared not to be complete after a few hours or days but continued over two weeks
p.e. This was due to the additional formation of salt layers on the [Ag-105]AgNP
surface that mediate and prolonge the dissolution process. The concurrent clearance
of persistent cores of [Ag-105]AgNP and [Ag-105]Ag-salt precipitates results in the
elimination of a fraction > 0.8 (per ILD) after one week, each particulate Ag-
species accounting for about half of this. After 28 days p.e. the cleared fraction
rises marginally to 0.94 while 2/3 of the remaining [Ag-105]AgNP are retained in
the lungs and 1/3 in secondary organs and tissues with an unknown partition of the
Ag species involved. However, making use of our previous biokinetics studies of
poorly soluble [Au-195]AuNP of the same size and under identical experimental and
exposure conditions (Kreyling et al., ACS Nano 2018), the kinetics of the ABB-
translocation of [Ag-105]Ag-salt precipitates was estimated to reach a fractional
maximum of 0.12 at day 3 p.e. and became undetectable 16 days p.e. Hence,
persistent cores of [Ag-105]AgNP were cleared throughout the study period. Urinary
[Ag-105]Ag excretion is minimal, finally accumulating to 0.016. Conclusion The
biokinetics of inhaled [Ag-105]AgNP is relatively complex since the dissolving [Ag-
105]Ag-ions (a) form salt layers on the [Ag-105]AgNP surface which retard
dissolution and (b) the [Ag-105]Ag-ions released from the [Ag-105]AgNP surface form
poorly-soluble precipitates of [Ag-105]Ag-salts in ELF. Therefore, hardly any [Ag-
105]Ag-ion clearance occurs from the lungs but instead [Ag-105]AgNP and nano-sized
precipitated [Ag-105]Ag-salt are cleared via the larynx into GIT and, in addition,
via blood, liver, gall bladder into GIT with one common excretional pathway via
feces out of the body.
AN - WOS:000540227300001
AU - Holzwarth, U.
AU - Hirn, S.
AU - Schleh, C.
AU - Wenk, A.
AU - Schaffler, M.
AU - Haberl, N.
AU - Gibson, N.
C7 - 21
DA - JUN 5
DO - 10.1186/s12989-020-00347-1
IS - 1
PY - 2020
SN - 1743-8977
ST - Quantitative biokinetics over a 28 day period of freshly generated, pristine,
20 nm silver nanoparticle aerosols in healthy adult rats after a single 11/2-hour
inhalation exposure
TI - Quantitative biokinetics over a 28 day period of freshly generated, pristine,
20 nm silver nanoparticle aerosols in healthy adult rats after a single 11/2-hour
inhalation exposure
VL - 17
ID - 6505
ER -
TY - JOUR
AB - The sequence of biochemical and cellular responses restoring the integrity of
the subcutaneous tissue of the skin is termed as wound healing. Inflammatory
cytokine suppression and inflammatory transduction cas-cades are the major targets
for wound healing. Formulations for wound healing should promote neovasculariza-
tion and angiogenic pathways by increasing the expression of vascular endothelial
growth factor, fibroblast growth factor, and platelet-derived growth factor.
Medication used for wound healing promotes anti-inflammatory associated with anti-
bacterial action. In order to boost the effectiveness of current medical treat-
ments, the cutting-edge nanotechnology offers many novel therapies. This review
summarized and discussed wound healing, types of wounds, natural materials used for
wound healing, metallic nanoparticles and current nano drug delivery systems used
for wound healing with special emphasis on the angiogenesis role in the healing of
wounds. © 2020 Bentham Science Publishers.
AU - Krishnaswami, V.
AU - Raju, N. S.
AU - Alagarsamy, S.
AU - Kandasamy, R.
DB - Scopus
DO - 10.2174/1381612826666200701203432
IS - 36
KW - Angiogenesis
Healing
Nano-drug
Treatment
Wound
Humans
Platelet-Derived Growth Factor
Skin
Wound Healing
copper nanoparticle
gold nanoparticle
hydrogel
nanocarrier
nanocomposite
nanofiber
silica nanoparticle
silver nanoparticle
vasculotropin A
acute wound
Aegle marmelos
Aloe vera
angiogenesis
Article
Azadirachta indica
biocompatibility
chronic wound
clinical evaluation
closed wound
drug formulation
Gmelina arborea
human
hypoxia
Moringa oleifera
nanoemulsion
Ocimum tenuiflorum
open wound
priority journal
tissue regeneration
wound
wound healing
skin
M3 - Article
PY - 2020
SP - 4591-4600
ST - Novel nanocarriers for the treatment of wound healing
TI - Novel nanocarriers for the treatment of wound healing
85090844393&doi=10.2174%2f1381612826666200701203432&partnerID=40&md5=1cb170aabc5f60
621413fb74618301a5
VL - 26
ID - 5284
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are the most widely studied antimicrobial
nanomaterials. However, their use in biomedicine is currently limited due to the
availability of data that prove the nanosilver toxicity associated primarily with
oxidative stress development in mammalian cells. The surface modification of AgNPs
is a potent technique of improvement of their biocompatibility. The synthetic or
natural compounds that combine zero or low toxicity towards human and animal
organisms with inherent antimicrobial properties are the most promising stabilizing
agents, their use would also minimize the risks of microorganisms developing
resistance to silver-based materials. We used a simple technique to obtain 30-60 nm
AgNPs stabilized with benzyldimethyl[3-myristoylamine)-propyl]ammonium chloride
monohydrate (BAC)-a well-known active ingredient of many antibacterial drugs. The
objective of the study was to assess the AgNPs-BAC entero- and hepatotoxicity to
CBF1 mice upon enteral administration. The animals were exposed to 0.8-7.5 mg/kg
doses of AgNPs-BAC in the acute and to 0.05-2.25 mg/kg doses of AgNPs-BAC in the
subacute experiments. No significant entero- and hepatotoxic effects following a
single exposure to doses smaller than 4 mg/kg were detected. Repeated exposure to
the doses of AgNPs-BAC below 0.45 mg/kg and to the doses of BAC below 0.5 mg/kg
upon enteral administration also led to no adverse effects. During the acute
experiment, the higher AgNPs-BAC dose resulted in increased quantities of
aminotransferases and urea, as well as the albumin-globulin ratio shift, which are
indicative of inflammatory processes. Besides, the relative mass of the liver of
mice was smaller compared to the control. During the subacute experiment, the
groups treated with the 0.25-2.25 mg/kg dose of AgNPs-BAC had a lower weight gain
rate compared to the control, while the groups treated with the 2.25 mg/kg dose of
AgNPs-BAC showed statistically significant variations in the blood serum
transaminases activity, which indicated hepatosis. It should be noted that the
spleen and liver of the animals from the groups treated with the 0.45 and 2.25
mg/kg dose of AgNPs-BAC were more than two times smaller compared to the control.
In the intestines of some animals from the group treated with the 2.25 mg/kg dose
of AgNPs-BAC small areas of hyperemia and enlarged Peyer's patches were observed.
Histological examination confirmed the initial stages of the liver and intestinal
wall inflammation.
AN - WOS:000622908300001
AU - Krutyakov, Y. A.
AU - Kudrinskiy, A. A.
AU - Kuzmin, V. A.
AU - Pyee, J.
AU - Gusev, A. A.
AU - Vasyukova, I. A.
AU - Zakharova, O. V.
AU - Lisichkin, G. V.
C7 - 332
DA - FEB
DO - 10.3390/nano11020332
IS - 2
PY - 2021
SN - 2079-4991
ST - In Vivo Study of Entero- and Hepatotoxicity of Silver Nanoparticles
Stabilized with Benzyldimethyl-[3-myristoylamine)-propyl]ammonium Chloride
(Miramistin) to CBF1 Mice upon Enteral Administration
T2 - NANOMATERIALS
TI - In Vivo Study of Entero- and Hepatotoxicity of Silver Nanoparticles
Stabilized with Benzyldimethyl-[3-myristoylamine)-propyl]ammonium Chloride
(Miramistin) to CBF1 Mice upon Enteral Administration
VL - 11
ID - 6115
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are the most widely studied antimicrobial
nanomaterials. However, their use in biomedicine is currently limited due to the
availability of data that prove the nanosilver toxicity associated primarily with
oxidative stress development in mammalian cells. The surface modification of AgNPs
is a potent technique of improvement of their biocompatibility. The synthetic or
natural compounds that combine zero or low toxicity towards human and animal or-
ganisms with inherent antimicrobial properties are the most promising stabilizing
agents, their use would also minimize the risks of microorganisms developing
resistance to silver-based materials. We used a simple technique to obtain 30–60 nm
AgNPs stabilized with benzyldimethyl[3-myristoylamine)-propyl]ammonium chloride
monohydrate (BAC)—a well-known active ingredi-ent of many antibacterial drugs. The
objective of the study was to assess the AgNPs-BAC entero-and hepatotoxicity to
CBF1 mice upon enteral administration. The animals were exposed to 0.8–7.5 mg/kg
doses of AgNPs-BAC in the acute and to 0.05–2.25 mg/kg doses of AgNPs-BAC in the
sub-acute experiments. No significant entero-and hepatotoxic effects following a
single exposure to doses smaller than 4 mg/kg were detected. Repeated exposure to
the doses of AgNPs-BAC below 0.45 mg/kg and to the doses of BAC below 0.5 mg/kg
upon enteral administration also led to no adverse effects. During the acute
experiment, the higher AgNPs-BAC dose resulted in increased quantities of
aminotransferases and urea, as well as the albumin-globulin ratio shift, which are
in-dicative of inflammatory processes. Besides, the relative mass of the liver of
mice was smaller compared to the control. During the subacute experiment, the
groups treated with the 0.25–2.25 mg/kg dose of AgNPs-BAC had a lower weight gain
rate compared to the control, while the groups treated with the 2.25 mg/kg dose of
AgNPs-BAC showed statistically significant variations in the blood serum
transaminases activity, which indicated hepatosis. It should be noted that the
spleen and liver of the animals from the groups treated with the 0.45 and 2.25
mg/kg dose of AgNPs-BAC were more than two times smaller compared to the control.
In the intestines of some animals from the group treated with the 2.25 mg/kg dose
of AgNPs-BAC small areas of hyperemia and enlarged Peyer’s patches were observed.
Histological examination confirmed the initial stages of the liver and intestinal
wall inflammation. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Krutyakov, Y. A.
AU - Kudrinskiy, A. A.
AU - Kuzmin, V. A.
AU - Pyee, J.
AU - Gusev, A. A.
AU - Lisichkin, G. V.
C7 - 332
DB - Scopus
DO - 10.3390/nano11020332
IS - 2
KW - Acute experiment
Benzyldimethyl[3-myristoylamine)-propyl]ammonium chloride monohydrate
Enterotoxicity
Hepatotoxicity
Subacute experiment
M3 - Article
PY - 2021
SP - 1-24
ST - In vivo study of entero-and hepatotoxicity of silver nanoparticles stabilized
with benzyldimethyl-[3-myristoylamine)-propyl]ammonium chloride (Miramistin) to
cbf1 mice upon enteral administration
T2 - Nanomaterials
TI - In vivo study of entero-and hepatotoxicity of silver nanoparticles stabilized
with benzyldimethyl-[3-myristoylamine)-propyl]ammonium chloride (Miramistin) to
cbf1 mice upon enteral administration
85099855759&doi=10.3390%2fnano11020332&partnerID=40&md5=f45d8a5eff4a0da503bc2a27f0a
75b20
VL - 11
ID - 5235
ER -
TY - JOUR
AB - The article investigates hardness of a spiral of a nanotructural spiral
stent. The stent biological and biomechanical compatibility was examined in the
course of drainage of the native gullet of rats. The degree of microcirculation
disorders and inflammatory changes in the gullet wall depended on hardness of the
stent spiral.
AU - Kulikovskyi, V. F.
AU - Shkodkin, S. V.
AU - Kolobov, Y. R.
AU - Chramov, G. V.
AU - Miroshnichenko, O. V.
AU - Lyubushkin, A. V.
DB - Scopus
IS - 6
KW - Biological compatibility
Biomechanical compatibility
Drainage
Inflammation
Medical implant
Stent
Rattus
nanocoating
silver nanoparticle
animal experiment
animal model
animal tissue
Article
biocompatibility
biomechanics
cell infiltration
controlled study
elasticity
equipment design
hardness
mediastinitis
microangiopathy
muscle hypertrophy
muscle injury
nanostructural spiral stent
nonhuman
rat
stent
surgical drainage
thickness
vascular patency
M3 - Article
PY - 2014
SP - 1457-1461
ST - Revisiting biological and biomechanical compatibility of surgical stents
TI - Revisiting biological and biomechanical compatibility of surgical stents
84911141479&partnerID=40&md5=9d1a9505551b3ec613b8341fb993f249
VL - 5
ID - 5566
ER -
TY - JOUR
AB - Use of nanoparticles have established benefits in a wide range of
applications, however, the effects of exposure to nanoparticles on health and the
environmental risks associated with the production and use of nanoparticles are
less well-established. The present study addresses this gap in knowledge by
examining, through a scoping review of the current literature, the effects of
nanoparticles on human health and the environment. We searched relevant databases
including Medline, Web of Science, ScienceDirect, Scopus, CINAHL, Embase, and SAGE
journals, as well as Google, Google Scholar, and grey literature from June 2021 to
July 2021. After removing duplicate articles, the title and abstracts of 1495
articles were first screened followed by the full-texts of 249 studies, and this
resulted in the inclusion of 117 studies in the presented review. In this
contribution we conclude that while nanoparticles offer distinct benefits in a
range of applications, they pose significant threats to humans and the environment.
Using several biological models and biomarkers, the included studies revealed the
toxic effects of nanoparticles (mainly zinc oxide, silicon dioxide, titanium
dioxide, silver, and carbon nanotubes) to include cell death, production of
oxidative stress, DNA damage, apoptosis, and induction of inflammatory responses.
Most of the included studies (65.81%) investigated inorganic-based nanoparticles.
In terms of biomarkers, most studies (76.9%) used immortalised cell lines, whiles
18.8% used primary cells as the biomarker for assessing human health effect of
nanoparticles. Biomarkers that were used for assessing environmental impact of
nanoparticles included soil samples and soybean seeds, zebrafish larvae, fish, and
Daphnia magna neonates. From the studies included in this work the United States
recorded the highest number of publications (n = 30, 25.64%), followed by China,
India, and Saudi Arabia recording the same number of publications (n = 8 each),
with 95.75% of the studies published from the year 2009. The majority of the
included studies (93.16%) assessed impact of nanoparticles on human health, and
95.7% used experimental study design. This shows a clear gap exists in examining
the impact of nanoparticles on the environment. © 2023, The Author(s).
AU - Kumah, E. A.
AU - Fopa, R. D.
AU - Harati, S.
AU - Boadu, P.
AU - Zohoori, F. V.
AU - Pak, T.
C7 - 1059
DB - Scopus
DO - 10.1186/s12889-023-15958-4
IS - 1
KW - Biomarkers
Cytotoxicity
Environmental health
Genotoxicity
Human health
Impact
Nanoparticles
Scoping review
Toxicity
Animals
Environment
Humans
Infant, Newborn
Nanotubes, Carbon
Zebrafish
biological marker
carbon nanotube
nanoparticle
animal
environment
human
newborn
zebra fish
M3 - Article
PY - 2023
ST - Human and environmental impacts of nanoparticles: a scoping review of the
current literature
T2 - BMC Public Health
TI - Human and environmental impacts of nanoparticles: a scoping review of the
current literature
85160882132&doi=10.1186%2fs12889-023-15958-
4&partnerID=40&md5=f2f5f53dcd4144e1d712a6679e28843a
VL - 23
ID - 4996
ER -
TY - JOUR
AB - Nanotechnology is an evolving interdisciplinary field of research
interspersing material science and nanobiotechnology. Nanoparticles are studied
extensively for their specific catalytic, magnetic, electronic, optical,
antimicrobial, theranostic, diagnosis, wound healing, and anti-inflammatory
properties. ZnO nanoparticles (NPs) have many applications owing to their unique
characteristics, which include low cost, nontoxicity, abundance in nature, and the
ability to prepare compounds with varying morphologies having different properties.
The main aim of the study is to biosynthesis of ZnO nanoparticles coated with
silver from the aqueous extract of Ganoderma lucidum (Curtis) P. Karst and to
evaluate its antidiabetic potential by performing alpha-glucosidase inhibition and
alpha-amylase inhibition assays and to evaluate the anticancer potential by
cytotoxicity (MTT) assay against human breast cancer MDA-MB 231 cell lines. The
biosynthesis of ZnO nanoparticles coated with Ag was characterized by UV-vis
spectroscopy, Fourier transform infrared spectroscopy, energy dispersive X-ray
analysis, scanning electron microscope, and transmission electron microscopy. An
increasing concentration in the biosynthesized ZnO nanoparticles coated with Ag
produces strong antidiabetic activity through enzyme inhibition effect and
anticancer activity through the reduction of cell viability. The present study
recommended that the "Biological" method of biological nanoparticle production is a
promising approach that allows synthesis in aqueous conditions, with low energy
requirements and low costs. In the future, the mycosynthesized nanoparticles might
be used in the medical arena to treat and prevent diseases.
AN - WOS:000905910100001
AU - Kumar, Dsrs
AU - Elango, N.
AU - Selvaraju, G. D.
AU - Matthew, P. A.
AU - Palanisamy, S.
AU - Cho, H. Y.
AU - Al Khattaf, F. S.
AU - Hatamleh, A. A.
AU - Roy, A. D.
C7 - 2798532
DA - DEC 23
DO - 10.1155/2022/2798532
PY - 2022
SN - 1687-4110
1687-4129
ST - Mycosynthesis of Zinc Oxide Nanoparticles Coated with Silver using Ganoderma
lucidum (Curtis) P. Karst and Its Evaluation of In Vitro Antidiabetic and
Anticancer Potential
T2 - JOURNAL OF NANOMATERIALS
TI - Mycosynthesis of Zinc Oxide Nanoparticles Coated with Silver using Ganoderma
VL - 2022
ID - 6478
ER -
TY - JOUR
AB - The mass casualties caused by the delta variant and the wave of the newer
“Omicron” variant of SARS-COV-2 in India have brought about great concern among
healthcare officials. The government and healthcare agencies are seeking effective
strategies to counter the pandemic. The application of nanotechnology and
repurposing of drugs are reported as promising approaches in the management of
COVID-19 disease. It has also immensely boomed the search for productive, re-
liable, cost-effective, and bio-assimilable alternative solutions. Since ancient
times, the traditional-ly employed Ayurvedic bhasmas have been used for diverse
infectious diseases, which are now employed as nanomedicine that could be applied
for managing COVID-19-related health anomalies. Like currently engineered metal
nanoparticles (NPs), the bhasma nanoparticles (BNPs) are also packed with unique
physicochemical properties, including multi-elemental nanocrystalline compo-sition,
size, shape, dissolution, surface charge, hydrophobicity, and multi-pathway
regulatory as well as modulatory effects. Because of these conformational and
configurational-based physico-chemical advantages, Bhasma NPs may have promising
potential to manage the COVID-19 pandemic and reduce the incidence of pneumonia-
like common lung infections in children as well as age-related inflammatory
diseases via immunomodulatory, anti-inflammatory, antiviral, and adju-vant-related
properties. © 2023 Bentham Science Publishers.
AU - Kumar, P.
AU - Jayakumar, R.
AU - Dash, M. K.
AU - Joshi, N.
C7 - e210322202483
DB - Scopus
DO - 10.2174/2215083808666220321145803
IS - 3
KW - Bhasma
gut microbiome
marana
nanocrystalline structure
nanoparticles
proinflammatory
transition metal
capsid protein
copper nanoparticle
cytokine
gold nanoparticle
interleukin 1beta
interleukin 2
interleukin 6
interleukin 8
metal nanoparticle
metalloprotein
nanoparticle
new drug
noradrenalin
RNA polymerase
silver nanoparticle
transition element
virus spike protein
zinc nanoparticle
adaptive immunity
antiviral activity
B lymphocyte
bioavailability
biocompatibility
biotransformation
CD4+ T lymphocyte
CD8+ T lymphocyte
child
coronavirus disease 2019
crystal structure
cytotoxicity
dendritic cell
diabetes mellitus
dissolution
elemental analysis
gene expression
half life time
homeostasis
human
humoral immunity
hydrophobicity
hyperlipidemia
incidence
inflammation
innate immunity
intestine flora
lipid peroxidation
lung injury
macrophage
metal poisoning
microbiome
nanotechnology
pandemic
particle size
pathogenesis
pharmacodynamics
physical chemistry
Review
Severe acute respiratory syndrome coronavirus 2
signal transduction
surface charge
T lymphocyte
thermodynamics
virus replication
wound healing
X ray diffraction
M3 - Review
PY - 2023
SP - 28-43
ST - The Potential Impact of Ayurvedic Traditional Bhasma on SARS-CoV-2-Induced
Pathogenesis
T2 - Current Traditional Medicine
TI - The Potential Impact of Ayurvedic Traditional Bhasma on SARS-CoV-2-Induced
Pathogenesis
85148954485&doi=10.2174%2f2215083808666220321145803&partnerID=40&md5=7ed7c7724615cc
f9dc21603c305e81cb
VL - 9
ID - 5016
ER -
TY - JOUR
AB - Naringenin (NAR) is one of the naturally occurring flavonoids found in citrus
fruits and exerts a wide variety of pharmacological activities. The clinical
relevance of naringenin is limited by its low solubility and minimal
bioavailability, owing to its largely hydrophobic ring structure. The aim of the
present study is to develop a novel naringenin nanoparticle system (NAR NP) using
simple nanoprecipitation technique with polyvinylpyrrolidone (PVP) as the
hydrophilic carrier. The synthesized nanoparticles were characterized using XRD,
FTIR, SEM and EDX. The characterization study revealed the nanoscale properties and
the interactions between NAR and PVP. In vivo toxicological evaluations were
carried out at various doses (1, 5, 10 & 50 mg/kg body wt) in male Sprague-Dawley
rats in comparison with silver nanoparticle (AgNP) at toxic concentration (50 mg/kg
body wt). The altered hepatotoxicity markers, hematology parameters and antioxidant
defense system were observed in AgNP-treated rats. But NAR NP - treated rats did
not show any biochemical alterations and improved the antioxidant defense indices
when compared to control group, by virtue of the pharmacological properties exerted
by NAR. The modulatory effect of NAR NP over inflammatory and stress signaling
cascades were confirmed by the normalized mRNA expressions of NF-kappa B, TNF-
alpha. and IL-6. The histopathological analysis of liver, kidney and heart
reinforce our findings. These studies provide preliminary answers to some of the
key biological issues raised over the use and safety of nanoparticles for
diagnostic and therapeutic applications. Consequently, we suggest that the safe NAR
NP can be used to reduce the dosage of NAR, improve its bioavailability and merits
further investigation for therapeutic applications. (C) 2016 Elsevier Ireland Ltd.
AN - WOS:000383526800013
AU - Kumar, R. P.
AU - Abraham, A.
DA - SEP 25
DO - 10.1016/j.cbi.2016.07.012
PY - 2016
SN - 0009-2797
1872-7786
SP - 110-118
ST - PVP- coated naringenin nanoparticles for biomedical applications - In vivo
toxicological evaluations
TI - PVP- coated naringenin nanoparticles for biomedical applications - In vivo
VL - 257
ID - 6615
ER -
TY - JOUR
AB - Naringenin (NAR) is one of the naturally occurring flavonoids found in citrus
fruits and exerts a wide variety of pharmacological activities. The clinical
relevance of naringenin is limited by its low solubility and minimal
bioavailability, owing to its largely hydrophobic ring structure. The aim of the
present study is to develop a novel naringenin nanoparticle system (NAR NP) using
simple nanoprecipitation technique with polyvinylpyrrolidone (PVP) as the
hydrophilic carrier. The synthesized nanoparticles were characterized using XRD,
FTIR, SEM and EDX. The characterization study revealed the nanoscale properties and
the interactions between NAR and PVP. In vivo toxicological evaluations were
carried out at various doses (1, 5, 10 & 50 mg/kg body wt) in male Sprague-Dawley
rats in comparison with silver nanoparticle (AgNP) at toxic concentration (50 mg/kg
body wt). The altered hepatotoxicity markers, hematology parameters and antioxidant
defense system were observed in AgNP- treated rats. But NAR NP – treated rats did
not show any biochemical alterations and improved the antioxidant defense indices
when compared to control group, by virtue of the pharmacological properties exerted
by NAR. The modulatory effect of NAR NP over inflammatory and stress signaling
cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-
6. The histopathological analysis of liver, kidney and heart reinforce our
findings. These studies provide preliminary answers to some of the key biological
issues raised over the use and safety of nanoparticles for diagnostic and
therapeutic applications. Consequently, we suggest that the safe NAR NP can be used
to reduce the dosage of NAR, improve its bioavailability and merits further
investigation for therapeutic applications. © 2016 Elsevier Ireland Ltd
AU - Kumar, R. P.
AU - Abraham, A.
DB - Scopus
DO - 10.1016/j.cbi.2016.07.012
KW - Antioxidant enzymes
Inflammatory markers
Nanoparticles
Naringenin
Polyvinylpyrrolidone
Toxicity markers
Animal Structures
Animals
Antioxidants
Biological Availability
Drug Carriers
Flavanones
Gene Expression
Inflammation
Male
Oxidative Stress
Patient Safety
Povidone
Rats
Silver
interleukin 6
messenger RNA
naringenin
povidone
silver nanoparticle
antioxidant
drug carrier
flavanone derivative
nanoparticle
silver
animal experiment
animal tissue
Article
bioavailability
controlled study
heart
histopathology
in vivo study
kidney
liver
male
material coating
nanoprecipitation
nonhuman
precipitation
rat
scanning electron microscope
signal transduction
X ray diffraction
animal
animal structures
drug effects
gene expression
inflammation
oxidative stress
patient safety
Sprague Dawley rat
standards
M3 - Article
PY - 2016
SP - 110-118
ST - PVP- coated naringenin nanoparticles for biomedical applications – In vivo
TI - PVP- coated naringenin nanoparticles for biomedical applications – In vivo
84982756961&doi=10.1016%2fj.cbi.2016.07.012&partnerID=40&md5=9e226f274bcf4501f45d5f
d5627327ab
VL - 257
ID - 5537
ER -
TY - JOUR
AB - Bio-neuronal led psychiatric abnormalities transpired by the loss of neuronal
structure and function (neurodegeneration), pro-inflammatory cytokines, microglial
dysfunction, altered neurotransmission, toxicants, serotonin deficiency, kynurenine
pathway, and excessively produced neurotoxic substances. These uncontrolled
happenings in the etiology of psychiatric disorders initiate further changes in
neurotransmitter metabolism, pathologic microglial, cell activation, and impaired
neuroplasticity. Inflammatory cytokines, the outcome of dysfunctional mitochondria,
dysregulation of the immune system, and under stress functions of the brain are
leading biochemical factors for depression and anxiety. Nanoscale drug delivery
platforms, inexpensive diagnostics using nanomaterials, nano-scale imaging
technologies, and ligand-conjugated nanocrystals used for elucidating the molecular
mechanisms and foremost cellular communications liable for such disorders are
highly capable features to study for efficient diagnosis and therapy of the mental
illness. These theranostic tools made up of multifunctional nanomaterials have the
potential for effective and accurate diagnosis, imaging of psychiatric disorders,
and are at the forefront of leading technologies in nanotheranostics openings field
as they can collectively and efficiently target the stimulated territories of the
cerebellum (cells and tissues) through molecular-scale interactions with higher
bioavailability, and bio-accessibility. Specifically, the nanoplatforms based
neurological changes are playing a significant role in the diagnosis of psychiatric
disorders and portraying the routes of functional restoration of mental disorders
by newer imaging tools at nano-level in all directions. Because of these
nanotherapeutic platforms, the molecules of nanomedicine can penetrate the Blood-
Brain Barrier with an increased half-life of drug molecules. The discoveries in
nanotheranostics and nanotherapeutics inbuilt unique multi-functionalities are
providing the best multiplicities of novel nanotherapeutic potentialities with no
toxicity concerns at the level of nano range. © The author(s).
AU - Kumar, R.
AU - Chhikara, B. S.
AU - Gulia, K.
AU - Chhillar, M.
DB - Scopus
DO - 10.7150/ntno.49619
IS - 3
KW - And kynurenine pathway
Microglial dysfunction
Nanotheranostics
Nanotherapeutics
Neurodegeneration
Psychiatric disorders
Blood-Brain Barrier
Humans
Inflammation
Mental Disorders
Nanomedicine
Precision Medicine
catalase
corticosteroid
glutathione
gold nanoparticle
hydrogel
inflammasome
kynurenine
liposome
nanocarrier
nanocrystal
neurotransmitter
nitric oxide
prostaglandin E2
serotonin
silver nanoparticle
bioavailability
blood brain barrier
brain function
confusion
delusion
drug formulation
drug release
electrophysiology
hallucination
human
mental disease
nerve cell network
neuroapoptosis
neuroimaging
neuromodulation
neuroprotection
neuropsychiatry
neurotransmission
Review
suicidal ideation
inflammation
nanomedicine
pathology
pathophysiology
M3 - Review
PY - 2021
SP - 288-308
ST - Review of nanotheranostics for molecular mechanisms underlying psychiatric
disorders and commensurate nanotherapeutics for neuropsychiatry: The mind knockout
T2 - Nanotheranostics
TI - Review of nanotheranostics for molecular mechanisms underlying psychiatric
disorders and commensurate nanotherapeutics for neuropsychiatry: The mind knockout
85103146828&doi=10.7150%2fntno.49619&partnerID=40&md5=39aace7298e3838a51cc270c4c203
ccb
VL - 5
ID - 5163
ER -
TY - JOUR
AB - The increased biomedical applications of enzyme-mimicking nanoparticles, also
known as ‘nanozymes’ has necessitated their controlled design in terms of
physicochemical properties, and reduced toxicity. In this context, numerous
approaches have been established to manufacture nanozymes using eco-friendly
routes, however, controlled surfaces and suitable functionalization of nanozymes
need much attention to utilize their full potential. Here, serine synthesised
silver (Ag) nanozymes have been fabricated, and sequentially surface functionalized
with isonicotinic acid hydrazide (isoniazid), streptomycin, and phosphomolybdic
acid to control their nanozyme-mimicking nature, and impart biological
capabilities. Post-physicochemical characterization, all these functional Ag-
centred nanozymes were explored for their inherent peroxidase enzyme-like behavior,
and haemcompatibility. The cell viability and proliferation assessments towards
mouse RAW 264.7 macrophages confirmed the cytocompatible nature of Ag nanozymes.
Moreover, these nanozymes show anti-inflammatory potential by regulating the key
inflammatory cytokines IL-6, IL-β, and TNF-α. © 2022 Elsevier B.V.
AU - Kumawat, M.
AU - Madhyastha, H.
AU - Singh, M.
AU - Jain, D.
AU - Daima, H. K.
C7 - 129294
DB - Scopus
DO - 10.1016/j.colsurfa.2022.129294
KW - Inflammatory cytokines
Isonicotinic acid hydrazide/isoniazid
Phosphomolybdic acid
Serine
Silver nanozymes
Streptomycin
Amines
Amino acids
Macrophages
Physicochemical properties
Pyridine
Silver
interleukin 1beta
interleukin 6
isoniazid
molybdic acid
peroxidase
phosphomolybdic acid
serine
silver nanoparticle
streptomycin
unclassified drug
Cytokine expression
Isonicotinic acid
Isonicotinic acid hydrazide/isonicotinic acid hydrazide
Silver nanozyme
animal cell
Article
biocompatibility
cell proliferation
cell viability
controlled study
cytokine production
down regulation
drug cytotoxicity
homeostasis
hydrodynamics
in vitro study
macrophage
mouse
nanoengineering
nanofabrication
nonhuman
physical chemistry
RAW 264.7 cell line
surface charge
synthesis
zeta potential
Enzymes
M3 - Article
PY - 2022
ST - Functional silver nanozymes regulate cell inflammatory cytokines expression
in mouse macrophages
TI - Functional silver nanozymes regulate cell inflammatory cytokines expression
in mouse macrophages
85132903506&doi=10.1016%2fj.colsurfa.2022.129294&partnerID=40&md5=cf2bbc62206317ea1
b153cdd4d0905bd
VL - 650
ID - 5123
ER -
TY - JOUR
AB - Infection, trauma, and autoimmunity trigger tissue inflammation, often
leading to pain and loss of function. Therefore, approaches to control inflammation
based on nanotechnology principles are being developed in addition to available
methods. The metal-based nanoparticles are particularly attractive due to the ease
of synthesis, control over physicochemical properties, and facile surface
modification with different types of molecules. Here, we report curcumin conjugated
silver (Cur-Ag) nanoparticles synthesis, followed by their surface
functionalization with isoniazid, tyrosine, and quercetin, leading to Cur-AgINH,
Cur-AgTyr, and Cur-AgQrc nanoparticles, respectively. These nanoparticles possess
radical scavenging capacity, haemocompatibility, and minimal cytotoxicity to
macrophages. Furthermore, the nanoparticles inhibited the secretion of pro-
inflammatory cytokines such as interleukin-6, tumor necrosis factor-α, and
interleukin-1β from macrophages stimulated by lipopolysaccharide (LPS). The
findings reveal that the careful design of surface corona of nanoparticles could be
critical to increasing their efficacy in biomedical applications. © 2022 Kumawat et
al. This is an open access article distributed under the terms of the Creative
Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original author and source are credited.
AU - Kumawat, M.
AU - Madhyastha, H.
AU - Singh, M.
AU - Revaprasadu, N.
AU - Srinivas, S. P.
AU - Daima, H. K.
C7 - e0276296
DB - Scopus
DO - 10.1371/journal.pone.0276296
IS - 10 October
KW - Curcumin
Cytokines
Homeostasis
Humans
Inflammation
Interleukin-1beta
Interleukin-6
Isoniazid
Lipopolysaccharides
Metal Nanoparticles
Nanoparticles
Quercetin
Silver
Tyrosine
curcumin
interleukin 1beta
interleukin 6
isoniazid
lipopolysaccharide
quercetin
silver nanoparticle
tyrosine
cytokine
metal nanoparticle
nanoparticle
silver
animal cell
Article
biocompatibility
cell inflammatory homeostasis
controlled study
cytokine release
drug activity
drug conjugation
drug cytotoxicity
hemocompatible
homeostasis
inflammation
macrophage
mouse
nonhuman
physical chemistry
radical scavenging capacity
RAW 264.7 cell line
surface functionalization
surface property
synthesis
chemistry
human
metabolism
M3 - Article
PY - 2022
ST - Double functionalized haemocompatible silver nanoparticles control cell
inflammatory homeostasis
T2 - PLoS ONE
TI - Double functionalized haemocompatible silver nanoparticles control cell
inflammatory homeostasis
85140417679&doi=10.1371%2fjournal.pone.0276296&partnerID=40&md5=9ee2771019eb29ee080
fa495f7b38a81
VL - 17
ID - 5153
ER -
TY - JOUR
AB - The smart design of nanoparticles with varying surfaces may open a new avenue
for potential biomedical applications. Consequently, several approaches have been
established for controlled synthesis to develop the unique physicochemical
properties of nanoparticles. However, many of the synthesis and functionalization
methods are chemical-based and might be toxic to limit the full potential of
nanoparticles. Here, curcumin (a plant-derived material) based synthesis of gold
(Au) nanoparticles, followed by the development of a suitable exterior corona using
isoniazid (INH, antibiotic), tyrosine (Tyr, amino acid), and quercetin (Qrc,
antioxidant), is reported. All these nanoparticles (Cur-Au, Cur-Au-INH, Cur-Au-Tyr,
and Cur-Au-Qrc) possess inherent peroxidase-mimicking natures depending on the
surface corona of respective nanoparticles, and they are found to be excellent
candidates for free radical scavenging action. The peroxidase-mimicking
nanoparticle interactions with red blood cells and mouse macrophages confirmed
their hemo- and biocompatible nature. Moreover, these surface-engineered Au
nanoparticles were found to be suitable in subsiding key pro-inflammatory cytokines
such as interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and
interleukin-1 beta (IL-1 beta). The inherent peroxidase-mimicking behavior and
anti-inflammatory potential without any significant toxicity of these nanoparticles
may open new prospects for nanomedicine.
AN - WOS:000763586100024
AU - Kumawat, M.
AU - Madhyastha, H.
AU - Umapathi, A.
AU - Singh, M.
AU - Revaprasadu, N.
AU - Daima, H. K.
C6 - JAN 2022
DA - FEB 8
DO - 10.1021/acs.langmuir.1c03088
IS - 5
PY - 2022
SN - 0743-7463
1520-5827
SP - 1877-1887
ST - Surface Engineered Peroxidase-Mimicking Gold Nanoparticles to Subside Cell
Inflammation
T2 - LANGMUIR
TI - Surface Engineered Peroxidase-Mimicking Gold Nanoparticles to Subside Cell
Inflammation
VL - 38
ID - 6472
ER -
TY - JOUR
AB - We have reported that β-catenin-accumulated crypts (BCAC), which do not have
the appearance of aberrant crypt foci (ACF) are possible colonic premalignant
lesions in rats. Suppression of the occurrence and advancement of such lesions
should have critical relevance to cancer prevention. This study examined whether
sulindac, a chemopreventive nonsteroidal anti-inflammatory drug is able to induce
apoptosis in such premalignant lesions. At 6 weeks of age, rats groups 1-3 were
given azoxymethane (AOM) (15 mg/kg-body weight) once weekly for 3 weeks. Two groups
were given sulindac in the diet (200 and 400 ppm), starting at 9 weeks of age. The
rats were sacrificed at the termination, and the colons were carefully examined.
The incidence and crypt multiplicity of BCAC and ACF were significantly less than
those of the control group. The effect of sulindac on the expression of BCAC was
greater than that on ACF. Exposure to sulindac significantly increased the
apoptotic index (terminal deoxynucleotide transferase dUTP nick-end labeling
(TUNEL)-positive cells) in BCAC. However, no significant increase of the index was
found in the case of ACF. These results suggest that the chemopreventive effect of
sulindac in rats is related to the induction of apoptosis in premalignant lesions.
Our results also provide additional evidence that BCAC are premalignant lesions in
colon carcinogenesis in rodents.
AU - Kuno, T.
AU - Yamada, Y.
AU - Hirose, Y.
AU - Katayama, M.
AU - Sakata, K.
AU - Hara, A.
AU - Saji, S.
AU - Mori, H.
DB - Scopus
DO - 10.1111/j.1349-7006.2002.tb02164.x
IS - 3
KW - Aberrant crypt foci
Apoptosis
Chemoprevention
Sulindac
β-catenin-accumulated crypts
Animals
Azoxymethane
beta Catenin
Carcinogens
Cell Division
Cell Nucleus
Colonic Neoplasms
Cytoskeletal Proteins
Diet
Incidence
Male
Nucleolus Organizer Region
Precancerous Conditions
Rats
Rats, Inbred F344
Silver
Trans-Activators
azoxymethane
beta catenin
deoxyuridine triphosphate pyrophosphatase
sulindac
animal experiment
animal model
animal tissue
apoptosis
article
cancer prevention
chemoprophylaxis
colon cancer
colon carcinogenesis
controlled study
drug exposure
histopathology
intestine crypt
male
nick end labeling
nonhuman
precancer
priority journal
protein expression
rat
M3 - Article
PY - 2002
SP - 242-246
ST - Induction of apoptosis by sulindac in azoxymethane-induced possible colonic
premalignant lesions in rats
T2 - Japanese Journal of Cancer Research
TI - Induction of apoptosis by sulindac in azoxymethane-induced possible colonic
premalignant lesions in rats
0036239402&doi=10.1111%2fj.1349-
7006.2002.tb02164.x&partnerID=40&md5=4457fe6bab66e014cde84d8e0dbc4646
VL - 93
ID - 5809
ER -
TY - CHAP
AB - Nanoparticles are mainly synthesized by chemical methods that usually involve
toxic reactants as reducing agents that further produce toxic by-products, which in
turn are hazardous to the environment. During the last decade, the development of
eco-friendly alternative chemical methods based on microorganisms like bacteria or
fungi, or more recently biological molecules extracted from plants, have spurred a
lot of interest as it brings forward a solution to curb the production of toxic by-
products. The purpose of these investigations is to combine the natural biocidal
properties of nanoparticles with specific properties derived from plant extracts,
not limited to anti-inflammatory or anti-cancer properties. The development of
antibacterial/antifungal coatings based on nanoparticles exhibiting biocidal
properties offers a method to overpower multi-drug resistant bacteria. Metal oxide
nanoparticles (TiO 2 , ZnO, and MgO) and noble metal nanoparticles like Ag, Au, and
Pt have shown efficient biocidal properties against microorganisms. However, these
mechanisms are still not fully understood and moreover, depend on the type of
nanoparticles. Antibacterial activity of metal oxide nanoparticles is mainly
attributed to the production of reactive oxygen species whereas metal nanoparticles
interact with surface membranes and are capable of infiltrating into bacteria
whereupon they induce apoptosis. Here, the mechanism of preventing bacterial growth
is different from conventional antibiotics and offers a promising alternative. ©
2018 Elsevier Inc. All rights reserved.
AU - Küünal, S.
AU - Rauwel, P.
AU - Rauwel, E.
DB - Scopus
DO - 10.1016/B978-0-323-51254-1.00014-2
KW - Antibacterial coatings
Antifungal
Biocidal properties
Green synthesis
Noble metal nanoparticles
Plant extracts
Bacteria
Cell death
Drug delivery
Gold compounds
Inorganic coatings
Magnesia
Precious metals
Reducing agents
Silver compounds
TiO2 nanoparticles
Titanium dioxide
Zinc oxide
Anti-fungal
Metal nanoparticles
PY - 2018
SP - 411-446
ST - Plant extract mediated synthesis of nanoparticles
T2 - Emerging Applications of Nanoparticles and Architectural Nanostructures:
Current Prospects and Future Trends
TI - Plant extract mediated synthesis of nanoparticles
85054374489&doi=10.1016%2fB978-0-323-51254-1.00014-
2&partnerID=40&md5=2ab80e144b06620db4ca362565b2a3bf
ID - 5491
ER -
TY - JOUR
AB - Background: Continuous glucose measurements provide improved glycemic control
and may prevent hypoglycemia and long-term complications of diabetes. One of the
most promising techniques is the short-term implantation of electrochemical glucose
sensors in subcutis. However, the inflammatory reaction to these sensors may lead
to bioinstability of sensor measurements. The purpose of the present investigation
was to examine factors contributing to the observed subcutaneous inflammatory
reaction to an enzyme-based electrochemical glucose sensor for continuous glucose
measurements. The sensor biocompatibility was assessed in vitro and in vivo.
Methods: A toxicological assessment was performed on sensor materials and
leachables, and the endotoxin content of sensors was determined by a Limulus
amoebocyte lysate (LAL) test. Moreover, as a consequence of permanent penetration
of the skin by the sensor the role of bacterial migration to the tissue was
investigated. In vivo biocompatibility was investigated through histological
examination of implanted sensor membranes for 3 days in pigs. Additionally, the
effect of needle size and type (normal vs. inserter needle) on tissue trauma at
sensor insertion was evaluated, and the healing of subcutis was assessed
histologically from 3 to 14 days after removal of sensors. Results: The
toxicological assessment and the LAL test showed no concerns in a 3-day
implantation scenario, and bacterial migration to the subcutis could not be
detected. The histological examination showed that a reduction in needle size
reduced the extent of inflammation to very low levels, and that the different
sensor membranes showed similar extent and type of inflammation. Additionally, the
extent of subcutaneous tissue reaction after removal of sensors declined gradually
over time and returned to near-normal levels after 2 weeks. Conclusion: The
electrochemical enzyme-based glucose sensor for continuous glucose measurements in
subcutis is acceptable from a biocompatibility point of view. Reducing the inserter
needle in size reduces the trauma induced at sensor implantation to neglible
levels. Furthermore, the tissue reaction to the sensor returns to near-normal 2
weeks after the sensor has been removed following a 3-day implantation period. ©
Mary Ann Liebert, Inc.
AU - Kvist, P. H.
AU - Iburg, T.
AU - Aalbaek, B.
AU - Gerstenberg, M.
AU - Schoier, C.
AU - Kaastrup, P.
AU - Buch-Rasmussen, T.
AU - Hasselager, E.
AU - Jensen, H. E.
DB - Scopus
DO - 10.1089/dia.2006.8.546
IS - 5
KW - Animals
Blood Glucose
Electrochemistry
Endotoxins
Female
Glucose Oxidase
Horseshoe Crabs
Materials Testing
Monitoring, Ambulatory
Subcutaneous Tissue
Swine
cellulose triacetate
dimeticone
enzyme
glucose
glucose oxidase
glutaraldehyde
platinum
polymer
silver
silver chloride
animal cell
animal experiment
animal model
animal tissue
article
biocompatibility
electrochemistry
electronic sensor
female
glucose transducer
hypoglycemia
in vitro study
in vivo study
inflammation
Limulus lysate test
nonhuman
priority journal
skin penetration
tissue reaction
wound healing
M3 - Article
PY - 2006
SP - 546-559
ST - Biocompatibility of an enzyme-based, electrochemical glucose sensor for
short-term implantation in the subcutis
T2 - Diabetes Technology and Therapeutics
TI - Biocompatibility of an enzyme-based, electrochemical glucose sensor for
short-term implantation in the subcutis
33845673743&doi=10.1089%2fdia.2006.8.546&partnerID=40&md5=8058736920250098da20bde65
f6a42ef
VL - 8
ID - 5830
ER -
TY - JOUR
AB - Recently, palladium nanoparticles (Pd-NPs) have been shown to possess pro-
inflammatory activities. Herein, we investigated potential in vitro effects of Pd-
NPs (primary size of 1-10 nm) on the biology of neutrophils, key player cells in
inflammation. Also, the aim of this study was to evaluate the pro-inflammatory
activity of Pd-NPs using the murine air pouch model, a model previously proposed to
be used as a standard assay for testing in vivo pro-inflammatory effects of NPs.
Although the positive controls used in vitro give the expected results in all
biological functions tested, Pd-NPs do not affect the production of reactive oxygen
species and that of interleukin-1 beta (IL-1 beta), IL-6, and IL-8. Pd-NPs
moderately increase cellular adhesion of neutrophils onto human endothelial cells
and significantly increase the capacity of neutrophils to migrate and to delay
apoptosis. We conclude that Pd-NPs possess some pro-inflammatory activity in vitro
but do not attract leukocytes in vivo regardless of sex.
AN - WOS:000595295600002
AU - Kwemo, P.
AU - Saafane, A.
AU - Vanharen, M.
AU - Durocher, I.
AU - Girard, D.
C7 - 350
DA - NOV
DO - 10.1007/s11051-020-05079-z
IS - 11
PY - 2020
SN - 1388-0764
1572-896X
ST - Impact of palladium nanoparticles (Pd-NPs) on the biology of neutrophils in
vitro and on leukocyte attraction in vivo
TI - Impact of palladium nanoparticles (Pd-NPs) on the biology of neutrophils in
vitro and on leukocyte attraction in vivo
VL - 22
ID - 6702
ER -
TY - JOUR
AB - The synthesis and characterization of the silver(I) chloride complex of
formula {[AgCI(CMBZT)(TPTP)(2)] center dot (MeOH)} (1) (CMBZT = 5-chloro-2-
mercaptobenzothiazole, TPTP = tris(p-tolyl) phosphine) is described. Also the
structure of the hydrate derivative {[AgCI(TPTP)(3)] center dot (0.5 center dot
H2O)} (2) of the corresponding known anhydrous silver complex (Zartilas et al.,
2009), and the polymorph 3 of the known [AgI(TPTP)(3)] complex (Zartilas et al.,
2009) were determined and compared with the known ones. In addition, the structure
of the known one silver(I) cluster {[AgI(TPTP)](4)} (4) (Meijboom et al., 2009) was
re-determined at 120(2) K and possible Ag-Ag interactions were analyzed. The
compounds 1-4 were characterized by X-ray crystallography at r.t (1) and 120 K (2-
4). All these complexes and {[(Et3NH)(+)](2) center dot [Ag-6(mu(3)-Hmna)(4)(mu(3)-
mna)(2)](2-) center dot (DMSO)(2) center dot (H2O)} (5) (Hmna = 2-mercaptonicotinic
acid) were evaluated for cytotoxic and anti-inflammatory activity. The in vitro
testing of cytotoxic activity of 1-5 against leiomyosarcoma cancer cells (LMS),
were evaluated with Trypan Blue and Thiazolyl Blue Tetrazolium Bromide or 3-(4.5-
dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assays. The flow
cytometry assay for complex 1 and showed that at 15 mu M of 1, 62.38% of LMS cells
undergo apoptosis, while 7% of LMS cells undergo cell necrosis. The antitumor
activity of 3 is comparable with that of its reported polymorph (Zartilas et al.,
2009). The anti-inflammatory, activity of complexes 1-3 and 5 was also studied. The
activity towards cell viability was 2 > 3 > 5 > 1 > 4, while the order of the
inhibitory activity in cell growth proliferation follows the order, 2 > 3 > 1 > 4 >
5. The anti-inflammatory activity on the other hand is 1 > 2 > 5 > ... > 3.
Copyright (C) 2010 L. Kyros et al.
AN - WOS:000277685000001
AU - Kyros, L.
AU - Kubicki, M.
AU - Male, L.
AU - Hursthouse, M. B.
AU - Verginadis, II
AU - Gouma, E.
AU - Karkabounas, S.
C7 - 386860
DO - 10.1155/2010/386860
PY - 2010
SN - 1565-3633
1687-479X
ST - Structural Properties, Cytotoxicity, and Anti-Inflammatory Activity of
Silver(I) Complexes with tris(p-tolyl)Phosphine and 5-Chloro-2-
Mercaptobenzothiazole
T2 - BIOINORGANIC CHEMISTRY AND APPLICATIONS
TI - Structural Properties, Cytotoxicity, and Anti-Inflammatory Activity of
Silver(I) Complexes with tris(p-tolyl)Phosphine and 5-Chloro-2-
Mercaptobenzothiazole
VL - 2010
ID - 6051
ER -
TY - JOUR
AB - Purpose of review Systemic lupus erythematosus is characterized by overactive
cells that differentiate into autoantibody-forming cells, aberrant T cell function
that provides help to B cells, and the production of pro-inflammatory cytokines.
This article reviews recent studies unraveling the complex interplay between
cytokines and lymphocytes,in systemic lupus erythematosus. Recent findings In
systemic lupus erythematosus, T cells are characterized by heightened calcium
responses early after activation of their surface receptor. Alterations of the T
cell receptor/CD3 complex, namely the substitution of the Fc epsilon R gamma for
the T cell receptor zeta chain, and increased mitochondrial potentials can account
for this 'overexcitable' phenotype. At the same time, this heightened calcium
signal leads to a block of the transcription of the IL-2 gene, a pivotal cytokine
for the immune response. The end result is increased spontaneous apoptosis and
decreased activation-induced cell death of T cells in systemic lupus erythematosus
that in turn leads to I enhanced help to B cells and potentially decreased
regulatory function. The B cells, on the other hand, are shown to be directly
activated by immune complexes by way of Toll-like receptors independently of T
cells. Finally, recent studies have tried to elucidate the role of cytokines such
as-interferon-alpha in systemic lupus erythematosus and, following the paradigm of
rheumatoid arthritis, to establish targets for treatment. Summary The increased
apoptosis and aberrant T cell activation coupled with nonspecific activation of B
cells lead to the production of auto-antigen: auto-antibody complexes that are the
hallmark of systemic lupus erythematosus. Future treatments aiming at correcting
the intracellular and intercellular signaling abnormalities may prove effective in
restoring immune tolerance in systemic lupus erythematous.
AN - WOS:000231382800003
AU - Kyttaris, V. C.
AU - Juang, Y. T.
AU - Tsokos, G. C.
DA - SEP
DO - 10.1097/01.bor.0000170479.01451.ab
IS - 5
PY - 2005
SN - 1040-8711
1531-6963
SP - 518-522
ST - Immune cells and cytokines in systemic lupus erythematosus: an update
T2 - CURRENT OPINION IN RHEUMATOLOGY
TI - Immune cells and cytokines in systemic lupus erythematosus: an update
VL - 17
ID - 6517
ER -
TY - JOUR
AB - Parkinson's disease (PD) is a disabling progressive neurodegenerative
disease. So far, PD's treatment remains symptomatic with no curative effects. Aside
from its blatant analgesic and antipyretic efficacy, recent studies highlighted the
endowed neuroprotective potentials of paracetamol (PCM). To this end: the present
study investigated: (1) Possible protective role of PCM against rotenone-induced
PD-like neurotoxicity in rats, and (2) the mechanisms underlying its
neuroprotective actions including cannabinoid receptors’ modulation. A dose-
response study was conducted using three doses of PCM (25, 50, and 100 mg/kg/day,
i.p.) and their effects on body weight changes, spontaneous locomotor activity,
rotarod test, tyrosine hydroxylase (TH) and α-synuclein expression, and striatal
dopamine (DA) content were evaluated. Results revealed that PCM (100 mg/kg/day,
i.p.) halted PD motor impairment, prevented rotenone-induced weight loss, restored
normal histological tissue structure, reversed rotenone-induced reduction in TH
expression and striatal DA content, and markedly decreased midbrain and striatal α-
synuclein expression in rotenone-treated rats. Accordingly, PCM (100 mg/kg/day,
i.p.) was selected for further mechanistic investigations, where it ameliorated
rotenone-induced oxidative stress, neuro-inflammation, apoptosis, and disturbed
cannabinoid receptors’ expression. In conclusion, our findings imply a multi-target
neuroprotective effect of PCM in PD which could be attributed to its antioxidant,
anti-inflammatory and anti-apoptotic activities, in addition to cannabinoid
receptors’ modulation. © 2021 Elsevier B.V.
AU - Labib, A. Y.
AU - Ammar, R. M.
AU - El-Naga, R. N.
AU - El-Bahy, A. A. Z.
AU - Tadros, M. G.
AU - Michel, H. E.
C7 - 107431
DB - Scopus
DO - 10.1016/j.intimp.2021.107431
KW - Cannabinoid receptors
Paracetamol
Parkinson's disease
Rotenone
Acetaminophen
alpha-Synuclein
Animals
Apoptosis
Corpus Striatum
Dopamine
Endocannabinoids
Male
Mesencephalon
Oxidative Stress
Parkinsonian Disorders
Rats, Wistar
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
alpha synuclein
cannabinoid 1 receptor
cannabinoid 2 receptor
cannabinoid receptor
caspase 3
cyclooxygenase 2
cytochrome c
dopamine
endocannabinoid
glutathione
malonaldehyde
messenger RNA
paracetamol
rotenone
silver chloride
adult
animal experiment
animal model
animal tissue
apoptosis
Article
body weight change
body weight loss
chemoluminescence
controlled study
corpus striatum
drug efficacy
immunoreactivity
latency to fall
lipid peroxidation
locomotion
male
mesencephalon
motor coordination
motor dysfunction
neuroprotection
neurotoxicity
nonhuman
oxidative stress
Parkinson disease
priority journal
protein expression
rat
rotarod test
tissue structure
animal
drug effect
genetics
metabolism
parkinsonism
pathology
Wistar rat
M3 - Article
PY - 2021
ST - Mechanistic insights into the protective effect of paracetamol against
rotenone-induced Parkinson's disease in rats: Possible role of endocannabinoid
system modulation
T2 - International Immunopharmacology
TI - Mechanistic insights into the protective effect of paracetamol against
rotenone-induced Parkinson's disease in rats: Possible role of endocannabinoid
system modulation
85100794501&doi=10.1016%2fj.intimp.2021.107431&partnerID=40&md5=f22c067f76847ccc4ce
b53c4cdcbd124
VL - 94
ID - 5228
ER -
TY - JOUR
AB - Due to their distinctive physicochemical properties, platinum nanoparticles
(PtNPs) have emerged as a material of interest for a number of biomedical
therapeutics. However, in some instances NP exposure has been correlated to health
and safety concerns, including cytotoxicity, activation of cellular stress, and
modification to normal cell functionality. As PtNPs have induced differential
cellular responses in vitro, the goal of this study was to further characterize the
behavior and toxicological potential of PtNPs within a HepG2 liver model. This
study identified that a high PtNP dosage induced HepG2 cytotoxicity. However,
lower, subtoxic PtNP concentrations were able to elicit multiple stress responses,
secretion of proinflammatory cytokines, and modulation of insulin-like growth
factor-1 dependent signal transduction. Taken together, this work suggests that
PtNPs would not be overtly toxic for acute exposures, but sustained cellular
interactions might produce long term health consequences.
AN - WOS:000447544300001
AU - Labrador-Rached, C. J.
AU - Browning, R. T.
AU - Comfort, K. K.
C7 - 1367801
DO - 10.1155/2018/1367801
PY - 2018
SN - 1687-8191
1687-8205
ST - Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of
Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro
T2 - JOURNAL OF TOXICOLOGY
TI - Toxicological Implications of Platinum Nanoparticle Exposure: Stimulation of
Intracellular Stress, Inflammatory Response, and Akt Signaling In Vitro
VL - 2018
ID - 6533
ER -
TY - JOUR
AB - Approximately 95% of patients receiving radiotherapy (RI) will ultimately
develop radiation-induced dermatitis (RID) during or after the course of treatment,
with major consequences on quality of life and treatment outcomes. This paper
reviews the pathophysiology of RID and currently used topical products for the
prevention and treatment of RID. Although there is no consensus on the appropriate
management, recent evidence suggests that the use of topical products supports to
protect and promote tissue repair in patients with RID. Basic recommendations
include advice to wear loose clothing, using electric razors if necessary, and
avoiding cosmetic products, sun exposure or extreme temperatures. Based on
mechanisms involved and on the clinical characteristics of oncological patients,
the profile of the ideal topical product for addressing RID can be designed; it
should have limited risk of adverse events, systemic adsorption and drug-drug
interactions, should be characterized by multiple clinical activities, with a
special focus on localized pain, and should have a careful formulation as some
vehicles can block the RI beam. © 2020 Bioexcel Publishing LTD. All rights
reserved.
AU - Lacovelli, N. A.
AU - Torrente, Y.
AU - Ciuffreda, A.
AU - Guardamagna, V. A.
AU - Gentili, M.
AU - Giacomelli, L.
AU - Sacerdote, P.
C7 - 2020-4-7
DB - Scopus
DO - 10.7573/dic.2020-4-7
KW - Pain
Quality of life
Radiation-induced dermatitis
Radiotherapy
Skin toxicity
Topical treatment.
allantoin
Aloe vera extract
arginine
atorvastatin
Calendula extract
corticosteroid
gamma interferon inducible protein 10
glutamine
hyaluronic acid
Hypericum perforatum extract
interleukin 1alpha
interleukin 1beta
interleukin 6
interleukin 8
lanolin alcohol
mometasone furoate
neem oil
olive oil
recombinant granulocyte macrophage colony stimulating factor
sitosterol
steroid
sucralfate
sulfadiazine silver
unindexed drug
anus cancer
body mass
breast cancer
cancer radiotherapy
cytokine release
disease severity
genetic susceptibility
head and neck cancer
human
hygiene
incidence
larynx carcinoma
mouth carcinoma
nasopharynx carcinoma
nutritional status
pathophysiology
pharynx carcinoma
prevalence
psoriasis
quality of life
radiation dose
radiation exposure
radiation safety
rectum cancer
Review
risk factor
smoking
treatment duration
M3 - Review
PY - 2020
ST - Topical treatment of radiation-induced dermatitis: Current issues and
potential solutions
T2 - Drugs in Context
TI - Topical treatment of radiation-induced dermatitis: Current issues and
potential solutions
85089454730&doi=10.7573%2fdic.2020-4-
7&partnerID=40&md5=e7ea4ef07abe61549f2129fca5e6400d
VL - 9
ID - 5303
ER -
TY - JOUR
AB - Inflammasome-derived cytokines, IL-1 beta and IL-18, and complement cascade
have been independently implicated in the pathogenesis of tuberculosis (TB)-immune
reconstitution inflammatory syndrome (TB-IRIS), a complication affecting HIV+
individuals starting antiretroviral therapy (ART). Although sublytic deposition of
the membrane attack complex (MAC) has been shown to promote NLRP3 inflammasome
activation, it is unknown whether these pathways may cooperatively contribute to
TB-IRIS. To evaluate the activation of inflammasome, peripheral blood mononuclear
cells (PBMCs) from HIV-TB co-infected patients prior to ART and at the IRIS or
equivalent timepoint were incubated with a probe used to assess active caspase-
1/4/5 followed by screening of ASC (apoptosis-associated speck-like protein
containing a CARD domain) specks as a readout of inflammasome activation by imaging
flow cytometry. We found higher numbers of monocytes showing spontaneous caspase-
1/4/5(+)ASC-speck formation in TB-IRIS compared to TB non-IRIS patients. Moreover,
numbers of caspase-1/4/5(+)ASC-speck(+) monocytes positively correlated with IL-1
beta/IL-18 plasma levels. Besides increased systemic levels of C1q and C5a, TB-IRIS
patients also showed elevated C1q and C3 deposition on monocyte cell surface,
suggesting aberrant classical complement activation. A clustering tSNE analysis
revealed TB-IRIS patients are enriched in a CD14(high)CD16(-) monocyte population
that undergoes MAC deposition and caspase-1/4/5 activation compared to TB non-IRIS
patients, suggesting complement-associated inflammasome activation during IRIS
events. Accordingly, PBMCs from patients were more sensitive to ex-vivo complement-
mediated IL-1 beta secretion than healthy control cells in a NLRP3-dependent
manner. Therefore, our data suggest complement-associated inflammasome activation
may fuel the dysregulated TB-IRIS systemic inflammatory cascade and targeting this
pathway may represent a novel therapeutic approach for IRIS or related inflammatory
syndromes. Author summary Tuberculosis (TB) associated-immune reconstitution
inflammatory syndrome (TB-IRIS) is a clinical complication affecting HIV+
individuals previously co-infected with Mycobacterium tuberculosis (Mtb), upon
antiretroviral therapy (ART) initiation. TB-IRIS is characterized by an exacerbated
inflammatory response and can be associated with high morbidity and mortality rates
in resource-limited countries with high TB prevalence. So far, there is no targeted
TB-IRIS therapy, and corticosteroids are frequently used to prevent or alleviate
IRIS related-symptoms. Here we found inflammasome activation (i.e.
caspase1/4/5(+)ASC speck complex formation) on circulating classical
CD14(high)CD16(-) monocytes may contribute to TB-IRIS immunopathology, since it
correlates with pro-inflammatory cytokine plasma levels and its decay is associated
with dampening in IRIS-related symptoms promoted by anti-inflammatory therapy. We
also found TB-IRIS monocytes display higher surface complement deposition, being
more sensitive to external complement-mediated NLRP3 inflammasome activation than
healthy control cells. In fact, complement MAC molecule C9 and caspase-1/4/5
activation were associated on classical monocytes in TB-IRIS patients, suggesting
complement-mediated inflammasome activation may lead to a positive feedback loop in
the inflammatory responses observed in TB-IRIS. Therefore, our findings support
that complement-NLRP3/ASC/caspase1/4/5 axis may be considered as a potential target
for host-directed therapy of TB-IRIS.
AN - WOS:000636354200005
AU - Lage, S. L.
AU - Wong, C. S.
AU - Amaral, E. P.
AU - Sturdevant, D.
AU - Hsu, D. C.
AU - Rupert, A.
AU - Wilson, E. M. P.
AU - Qasba, S. S.
AU - Naqvi, N. S.
AU - Laidlaw, E.
AU - Lisco, A.
AU - Manion, M.
AU - Sereti, I.
C7 - e1009435
DA - MAR
DO - 10.1371/journal.ppat.1009435
IS - 3
PY - 2021
SN - 1553-7366
1553-7374
ST - Classical complement and inflammasome activation converge in
CD14(high)CD16(-) monocytes in HIV associated TB-immune reconstitution inflammatory
syndrome
T2 - PLOS PATHOGENS
TI - Classical complement and inflammasome activation converge in
CD14(high)CD16(-) monocytes in HIV associated TB-immune reconstitution inflammatory
syndrome
VL - 17
ID - 6561
ER -
TY - JOUR
AB - It has been reported that increased levels and activity of the heme
oxygenase-1 (HO-1) protein ameliorate tissue injuries. In the present study, we
investigated the effects and mechanisms of action of gold nanoparticles (AuNPs) on
HO-1 protein expression in human vascular endothelial cells (ECs). The AuNPs
induced HO-1 protein and mRNA expression in a concentration-and time-dependent
manner. The induction was reduced by the thiol-containing antioxidants, including
N-acetylcysteine and glutathione, but not by the non-thiol-containing antioxidants
and inhibitors that block the enzymes for intracellular reactive oxygen species
generation. The AuNPs enhanced Nrf2 protein levels but did not affect Nrf2 mRNA
expression. In response to the AuNP treatment, the cytosolic Nrf2 translocated to
the nucleus, and, concomitantly, Bach1 exited the nucleus and its tyrosine
phosphorylation increased. The chromatin immunoprecipitation assay revealed that
the translocated Nrf2 bound to the antioxidant-response element located in the E2
enhancer region of the HO-1 gene promoter and acted as a transcription factor.
Although N-acetylcysteine inhibited the AuNP-induced Nrf2 nuclear translocation,
the AuNPs did not promote intracellular reactive oxygen species production or
endoplasmic reticulum stress in the ECs. Knockdown of Nrf2 expression by RNA
interference significantly inhibited AuNP-induced HO-1 expression at the protein
and mRNA levels. In summary, AuNPs enhance the levels and nuclear translocation of
the Nrf2 protein and Bach1 export/tyrosine phosphorylation, leading to Nrf2 binding
to the HO-1 E2 enhancer promoter region to drive HO-1 expression in ECs. This
study, together with our parallel findings, demonstrates that AuNPs can act as an
HO-1 inducer, which may partially contribute to their anti-inflammatory bioactivity
in human vascular ECs.
AN - WOS:000361621300001
AU - Lai, T. H.
AU - Shieh, J. M.
AU - Tsou, C. J.
AU - Wu, W. B.
DO - 10.2147/IJN.S88514
PY - 2015
SN - 1178-2013
SP - 5925-5939
ST - Gold nanoparticles induce heme oxygenase-1 expression through Nrf2 activation
and Bach1 export in human vascular endothelial cells
TI - Gold nanoparticles induce heme oxygenase-1 expression through Nrf2 activation
and Bach1 export in human vascular endothelial cells
VL - 10
ID - 6576
ER -
TY - JOUR
AB - Green synthesis is an eco-friendly approach of nanoparticles fabrication
gaining momentum among researches. Nanoparticles are used immensely, due to its
small size, physical properties, orientation, which can apparently change the
performance of any other material when in proximity. Antibacterial, antioxidant,
antifungal, antiviral, anti-inflammatory activities of several nanoparticles
capable of wound healing make it a appropriate module for wound dressing materials.
Silver nanoparticles (SNPs) are recognized as well established antimicrobial and
antiseptic agents, thus considering it as a promising candidate for wound healing
process and other applications. Here we report an effective and biological approach
of a novel thin film preparation based on polyvinyl alcohol (PVA) with SNPs
generated within matrix using leaf extract of Aloe vera as a bioreducing agent.
Successful incorporation of SNPs into the polymer matrix, which was confirmed by
NTA analysis, TEM, SEM. The characterization results revealed that SNPs were found
in range of 10-40 nm. Evaluation of antimicrobial activity on Escherichia coli
(ATCC 39403), Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (clinical
isolates), and Candida albicans (diploid fungus) using agar cup and disc diffusion
method confirmed effective performance of the PVA-SNPs film. Average ZOI was
observed as 3+2mm, 3+2mm, 4+1mm and 4+1mm respectively. Thin film produced is
highly biocompatible to HaCat and L929 cell lines for a defined amount and hence
can be used as wound dressing materials. The method used in this study is greener,
simple, rapid, and cost effective for producing a biocompatible film with
profoundly variable applications in food packaging and health care industries. ©
2002-2011 IEEE.
AU - Lakkakula, J.
AU - Divakaran, D.
AU - Srivastava, R.
AU - Ingle, P.
AU - Gade, A.
AU - Raut, R.
DB - Scopus
DO - 10.1109/TNB.2022.3208310
IS - 3
KW - Aloe Vera
green synthesis
polyvinyl alcohol (PVA)
silver nanoparticles (SNPs)
wound dressing
Metal Nanoparticles
Polyvinyl Alcohol
Silver
Algae
Biocompatibility
Cell culture
Escherichia coli
Film growth
Film preparation
Metal nanoparticles
Polyvinyl alcohols
Thin films
antiinfective agent
metal nanoparticle
polyvinyl alcohol
silver
<italic xmlns:ali="
Green products
Green synthesis
Medium
Polyvinyl alcohol
Silver nanoparticle
Vera</italic>
Wound
Wound dressings
Xmlns:mml="
Xmlns:xlink="
Xmlns:xsi="
M3 - Article
PY - 2023
SP - 480-486
ST - In Situ Growth of Biocompatible Biogenic Silver Nanoparticles in Poly-Vinyl
Alcohol Thin Film Matrix
T2 - IEEE Transactions on Nanobioscience
TI - In Situ Growth of Biocompatible Biogenic Silver Nanoparticles in Poly-Vinyl
Alcohol Thin Film Matrix
85139434052&doi=10.1109%2fTNB.2022.3208310&partnerID=40&md5=405c18b72d879408b133917
bd6144cfc
VL - 22
ID - 4964
ER -
TY - JOUR
AB - Objectives: Using dental Ti implants has become a well-accepted and used
method for replacing missing dentition. It has become evident that in many cases
peri-implant inflammation develops. The objective was to create and evaluate the
antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can
help to prevent such processes if applied on the surface of dental implants.
Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron
Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II
procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1
cm2 Grade 2 titanium samples. The antibacterial effect was evaluated by culturing
Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and
comparing the results to that of glass as control and of pure titanium samples.
Alamar Blue assay was carried out to check cytotoxicity. Results: It was proved
that silver nanoparticles were present on the treated surfaces. The average
diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian
distribution, the the filling factor was 25%. Antibacterial evaluation revealed
that the nanoparticle covered samples had an antibacterial effect of 64.6% that was
statistically significant. Tests also proved that the nanoparticles are safely
anchored to the titanium surface and are not cytotoxic. Conclusion: Creating a
silver nanoparticle layer can be an option to add antibacterial features to the
implant surface and to help in the prevention of peri-implant inflammatory
processes. Recent studies demonstrated that silver nanoparticles can induce
pathology in mammal cells, thus safe fixation of the particles is essential to
prevent them from getting into the circulation. © 2019 Lampé et al.
AU - Lampé, I.
AU - Beke, D.
AU - Biri, S.
AU - Csarnovics, I.
AU - Csik, A.
AU - Dombrádi, Z.
AU - Hajdu, P.
AU - Hegedűs, V.
AU - Rácz, R.
AU - Varga, I.
AU - Hegedűs, C.
DB - Scopus
DO - 10.2147/IJN.S197782
KW - Antibacterial effect
Cytocompatibility
Geometrical model
Implants
Oxyde layer
Peri-implant inflammation
Physical vapor deposition
Animals
Cell Survival
Dental Implantation
Ions
Metal Nanoparticles
Particle Size
Silver
Stem Cells
Surface Properties
Titanium
glass
silver nanoparticle
titanium
antiinfective agent
ion
metal nanoparticle
silver
Article
bacterium culture
controlled study
cytotoxicity
material state
nonhuman
particle size
safety
surface property
animal
cell survival
chemistry
cytology
drug effect
procedures
stem cell
tooth implantation
ultrastructure
M3 - Article
PY - 2019
SP - 4709-4721
ST - Investigation of silver nanoparticles on titanium surface created by ion
implantation technology
TI - Investigation of silver nanoparticles on titanium surface created by ion
85069927512&doi=10.2147%2fIJN.S197782&partnerID=40&md5=79e405081d0ff7e51052dcda4b0b
9ff0
VL - 14
ID - 5429
ER -
TY - JOUR
AB - Objectives: Using dental Ti implants has become a well-accepted and used
method for replacing missing dentition. It has become evident that in many cases
peri-implant inflammation develops. The objective was to create and evaluate the
antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can
help to prevent such processes if applied on the surface of dental implants.
Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron
Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II
procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1
cm(2) Grade 2 titanium samples. The antibacterial effect was evaluated by culturing
Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and
comparing the results to that of glass as control and of pure titanium samples.
Alamar Blue assay was carried out to check cytotoxicity. Results: It was proved
that silver nanoparticles were present on the treated surfaces. The average
diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian
distribution, the the filling factor was 25%. Antibacterial evaluation revealed
that the nanoparticle covered samples had an antibacterial effect of 64.6% that was
statistically significant. Tests also proved that the nanoparticles are safely
anchored to the titanium surface and are not cytotoxic. Conclusion: Creating a
silver nanoparticle layer can be an option to add antibacterial features to the
implant surface and to help in the prevention of peri-implant inflammatory
processes. Recent studies demonstrated that silver nanoparticles can induce
pathology in mammal cells, thus safe fixation of the particles is essential to
prevent them from getting into the circulation.
AN - WOS:000473358900002
AU - Lampe, I.
AU - Beke, D.
AU - Biri, S.
AU - Csarnovics, I.
AU - Csik, A.
AU - Dombradi, Z.
AU - Hajdu, P.
AU - Hegedus, V.
AU - Racz, R.
AU - Varga, I.
AU - Hegedus, C.
DO - 10.2147/IJN.S197782
PY - 2019
SN - 1178-2013
SP - 4709-4721
ST - Investigation of silver nanoparticles on titanium surface created by ion
TI - Investigation of silver nanoparticles on titanium surface created by ion
VL - 14
ID - 6132
ER -
TY - JOUR
AB - Due to their characteristic physical, chemical and optical properties,
titanium dioxide and silver nanoparticles are attractive tools for use in a wide
range of applications. The use of nanoparticles for biological applications is,
however, dependent upon their biocompatibility with living cells. Because of the
importance of inflammation as a modulator of human health, the safe and efficacious
in vivo use of titanium dioxide and silver nanoparticles is inherently linked to a
favorable interaction with immune system cells. However, both titanium dioxide and
silver nanoparticles have demonstrated potential to exert immunomodulatory and
immunotoxic effects. Titanium dioxide and silver nanoparticles are readily
internalized by immune system cells, may accumulate in peripheral lymphoid organs,
and can influence multiple manifestations of immune cell activity. Although the
factors influencing the biocompatibility of titanium dioxide and silver
nanoparticles with immune system cells have not been fully elucidated, nanoparticle
core composition, size, concentration and the duration of cell exposure seem to be
important Because titanium dioxide and silver nanoparticles are widely utilized in
pharmaceutical, commercial and industrial products, it is vital that their effects
on human health and immune system function be more thoroughly evaluated. (C) 2015
AN - WOS:000366951300010
AU - Lappas, C. M.
DA - NOV
DO - 10.1016/j.fct.2015.05.015
PY - 2015
SN - 0278-6915
1873-6351
SP - 78-83
ST - The immunomodulatory effects of titanium dioxide and silver nanoparticles
TI - The immunomodulatory effects of titanium dioxide and silver nanoparticles
VL - 85
ID - 5898
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are commonly found in consumer products due to
their antimicrobial properties. This study evaluated the effects of AgNPs on the
murine macrophage cell line RAW 264.7 and human whole blood cell cultures (WBCs).
Effects of AgNPs on RAW cells were assessed in the presence or absence of
lipopolysaccharide (LPS). Effects of AgNPs on WBCs were monitored under basal
conditions and in the presence of either LPS or phytohaemmagglutinin (PHA). AgNPs
were cytotoxic to WBCs at 250 mu g/ml. Under basal conditions, RAW cells >= 62.5.
mu g/ml and WBCs > 25 mu g/ml AgNPs induced biomarkers associated with
inflammation. Under LPS stimulated conditions, 250 mu g/ml AgNP inhibited
biomarkers associated with inflammation for both cultures. Under basal conditions,
and in the presence of 250 mu g/ml AgNP, WBCs produced acquired immune system
cytokines IL-10 and IFN gamma. IL-10 synthesis by WBCs was partially inhibited by
250 mu g/ml AgNP in the presence of PHA. Proteome profiles of RAW cell supernatants
show that AgNPs modulate biomarkers associated with inflammation. WBCs proteome
analysis shows modulation of biomarkers associated with anti-inflammatory effects.
AN - WOS:000488810100010
AU - Lategan, K. L.
AU - Walters, C. R.
AU - Pool, E. J.
C7 - Pmid 30468660
DA - JAN 1
DO - 10.2741/4722
PY - 2019
SN - 1093-9946
1093-4715
SP - 347-365
ST - The effects of silver nanoparticles on RAW 264.7. Macrophages and human whole
blood cell cultures
T2 - FRONTIERS IN BIOSCIENCE-LANDMARK
TI - The effects of silver nanoparticles on RAW 264.7. Macrophages and human whole
blood cell cultures
VL - 24
ID - 5901
ER -
TY - JOUR
AB - An in vitro cell culture approach was evaluated for its ability to provide
data pertinent to the assessment of skin irritation potential. The hypothesis of
this approach is that a direct toxic insult to the epidermal keratinocyte in vivo
may lead to release of inflammatory mediators, which are responsible for initiation
of a local primary skin irritant reaction. This paper presents data on the
cytotoxic potential of a number of structurally unrelated chemicals (chloroform, 2-
methoxyethanol, 2-butoxyethylacetate, toluene, 1- butanol, acetaldehyde, n-hexane,
sodium dodecyl sulfate, benzalkonium chloride, silver nitrate, dibutyltin
dichloride and tributyltin chloride). Cytotoxicity (neutral red uptake and
intracellular acid phosphatase activity) of a number of structurally unrelated
chemicals, representative of a wide range of skin irritation potential, was
evaluated in cultures of rat and human epidermal keratinocytes. The sensitivity of
human and rat keratinocytes to the test chemicals was very similar, irrespective of
the endpoint of cytotoxicity. The neutral red uptake assay appeared more generally
applicable to the diverse range of chemical structures represented in this study,
since not all test chemicals elicited an early increase in intracellular acid
phosphatase activity. The results were very encouraging, as a good correlation was
evident between cytotoxicity in rat keratinocytes and the degree of erythema and
oedema associated with an in vivo skin irritant response in rabbits. Keratinocyte
cytotoxicity data may provide an indication of the potential of a chemical to
induce a severe skin irritant reaction, or if a chemical is more likely to be a
marginal or non-irritant. However, the data illustrate that such assays appear
unable to discriminate correctly between more subtle classes of irritancy, such as
non-irritant, mild, moderate or severe. Available human in vivo skin irritation
data were insufficient to conclude which cell type is preferable for evaluation of
human skin irritation potential.
AU - Lawrence, J. N.
AU - Starkey, S.
AU - Dickson, F. M.
AU - Benford, D. J.
DB - Scopus
DO - 10.1016/0887-2333(96)00005-7
IS - 3
KW - Animalia
Oryctolagus cuniculus
2 methoxyethanol
acetaldehyde
acid phosphatase
benzalkonium chloride
butanol
chloroform
dibutyltin dichloride
hexane
irritant agent
neutral red
silver nitrate
toluene
tributyltin chloride
animal cell
animal model
animal tissue
article
controlled study
drug cytotoxicity
edema
erythema
human
human cell
keratinocyte
mediator release
nonhuman
rabbit
rat
skin inflammation
skin irritation
skin toxicity
M3 - Article
PY - 1996
SP - 331-340
ST - Use of human and rat keratinocyte cultures to assess skin irritation
potential
TI - Use of human and rat keratinocyte cultures to assess skin irritation
potential
0029944994&doi=10.1016%2f0887-2333%2896%2900005-
7&partnerID=40&md5=702ebfa98aa19323249574f616d9a65f
VL - 10
ID - 5797
ER -
TY - JOUR
AB - Implants and prostheses are widely used to replace damaged tissues or to
treat various diseases. However, besides the risk of bacterial or fungal infection,
an inflammatory response usually occurs. Here, recent progress in the field of
anti-inflammatory biomaterials is described. Different materials and approaches are
used to decrease the inflammatory response, including hydrogels, nanoparticles,
implant surface coating by polymers, and a variety of systems for anti-inflammatory
drug delivery. Complex multifunctional systems dealing with inflammation, microbial
infection, bone regeneration, or angiogenesis are also described. New promising
stimuli-responsive systems, such as pH- and temperature-responsive materials, are
also being developed that would enable an “intelligent” antiinflammatory response
when the inflammation occurs. Together, different approaches hold promise for
creation of novel multifunctional smart materials allowing better implant
integration and tissue regeneration. © 2020 Wiley-VCH GmbH
AU - Lebaudy, E.
AU - Fournel, S.
AU - Lavalle, P.
AU - Vrana, N. E.
AU - Gribova, V.
C7 - 2001373
DB - Scopus
DO - 10.1002/adhm.202001373
IS - 1
KW - anti-inflammatory material designs
biomaterials
coatings
hydrogels
nanoparticles
Hydrogels
Nanoparticles
Polymers
Pathology
Tissue
Tissue regeneration
aceclofenac
alginic acid
alpha tocopherol
amfenac
ascorbic acid
benzoic acid
betamethasone
betamethasone dipropionate
biomaterial
carbomer
catestatin
celecoxib
chitosan
chondroitin sulfate
citral
collagen
curcumin
cyclodextrin
dermatan sulfate
dexamethasone
diclofenac
dopamine
fibrin
fucoidin
gelatin
glycyrrhizic acid
heparin
hyaluronic acid
hydrogel
hydroxypropylcellulose
ibuprofen
indometacin
interleukin 1
interleukin 10
interleukin 13
interleukin 1beta
interleukin 4
interleukin 6
interleukin 8
keratin
ketoprofen
levofloxacin
lidocaine
luteolin 7 glucoside
metal nanoparticle
microgel (material)
morin
Moringa oleifera extract
nanocage
nanocapsule
nanomaterial
nanoparticle
naringenin
nerolidol
pilocarpine
piroxicam
plasmid DNA
polyarginine
polycaprolactone
polyetheretherketone
polyethylene
polyglutamic acid
polylactide
polysaccharide
polystyrene
prednisolone
quercetin
resolvin D1
resveratrol
silk
silver nanoparticle
titanium
unclassified drug
polymer
Anti-inflammatories
Anti-inflammatory response
Microbial infections
Multifunctional systems
PH- and temperature-responsive
Stimuli-responsive systems
Aloe vera
arthritis
Article
biocompatibility
biodegradation
coating (procedure)
cytokine production
dermatitis
drug release
drug solubility
equipment design
honey
human
hydrophobicity
immune response
immunomodulation
implantation
inflammation
knee arthritis
macromolecule
macrophage
mouth injury
nanoencapsulation
nanofabrication
nonhuman
periodontitis
physical chemistry
porosity
priority journal
regenerative medicine
surface property
tissue engineering
tissue regeneration
wound healing
Drug delivery
M3 - Article
PY - 2021
ST - Recent Advances in Antiinflammatory Material Design
TI - Recent Advances in Antiinflammatory Material Design
85092539540&doi=10.1002%2fadhm.202001373&partnerID=40&md5=b5199e0df15e0720bd514473b
d6f98da
VL - 10
ID - 5266
ER -
TY - JOUR
AB - Aims: This study explored whether silver nanoparticles (AgNPs) can disrupt
tight-junctions integrity resulted in blood-brain barrier dysfunction along with
oxidative stress, pro-inflammation, and apoptosis induction. Additionally,
neuroprotective activities of a-lipoic acid (LA) and Ginkgo biloba (GB) were
investigated. Main methods: Forty adults rats were enrolled into; Control, AgNPs
(50 mg/kg), LA (100 mg/kg) + AgNPs, and GB (120 mg/kg) + AgNPs. After 30 days,
neuronal changes were assessed biochemically and histopathologically. Brain tissues
oxidative indices, mRNA expression of proinflammatory cytokines and tight-junction
proteins and pro-apoptotic biomarker, caspase-3 were investigated. Key findings:
AgNPs exposure enhanced lipid peroxidation (+195%) along with declines in
glutathione (-43%), glutathione peroxidase (-34%), glutathione S-transferase (-
31%), catalase (-43%), and superoxide dismutase (-38%) activities in brain tissues.
The apparent brain oxidative damage was associated with obvious neuronal
dysfunction that was ascertained by neuropathological lesions. AgNPs lowered serum
acetylcholine esterase, iron and copper levels, and increased creatine
phosphokinase and creatine phosphokinase-brain type activities. Following AgNPs
exposure, brain silver and iron contents were increased, but the copper level was
decreased. AgNPs up-regulated TNF-alpha (6.5-fold) and IL-1 beta (8.9-fold)
transcript levels, and simultaneously over-expressed the caspase-3 protein in
cerebrum and cerebellum inducing cell apoptosis. Moreover, AgNPs down-regulated the
transcript levels of tight-junction proteins; JP-1 (0.65-fold) and JAM-3(0.81-
fold). Significance: LA and relatively GB improved the serious effects of AgNPs on
the blood-brain barrier function and tight-junction proteins through their
antioxidants, anti-inflammatory, and anti-apoptotic efficacies. Co-treatment with
LA or GB may be favorable in ameliorating the neurotoxic side effects of AgNPs.
AN - WOS:000447649400028
AU - Lebda, M. A.
AU - Sadek, K. M.
AU - Tohamy, H. G.
AU - Abouzed, T. K.
AU - Shukry, M.
AU - Umezawa, M.
AU - El-Sayed, Y. S.
DA - NOV 1
DO - 10.1016/j.lfs.2018.10.011
PY - 2018
SN - 0024-3205
1879-0631
SP - 251-260
ST - Potential role of alpha-lipoic acid and Ginkgo biloba against silver
nanoparticles-induced neuronal apoptosis and blood-brain barrier impairments in
rats
T2 - LIFE SCIENCES
TI - Potential role of alpha-lipoic acid and Ginkgo biloba against silver
rats
VL - 212
ID - 5936
ER -
TY - JOUR
AB - Aims: This study explored whether silver nanoparticles (AgNPs) can disrupt
tight-junctions integrity resulted in blood-brain barrier dysfunction along with
oxidative stress, pro-inflammation, and apoptosis induction. Additionally,
neuroprotective activities of α-lipoic acid (LA) and Ginkgo biloba (GB) were
investigated. Main methods: Forty adults rats were enrolled into; Control, AgNPs
(50 mg/kg), LA (100 mg/kg) + AgNPs, and GB (120 mg/kg) + AgNPs. After 30 days,
neuronal changes were assessed biochemically and histopathologically. Brain tissues
oxidative indices, mRNA expression of proinflammatory cytokines and tight-junction
proteins and pro-apoptotic biomarker, caspase-3 were investigated. Key findings:
AgNPs exposure enhanced lipid peroxidation (+195%) along with declines in
glutathione (−43%), glutathione peroxidase (−34%), glutathione S-transferase
(−31%), catalase (−43%), and superoxide dismutase (−38%) activities in brain
tissues. The apparent brain oxidative damage was associated with obvious neuronal
dysfunction that was ascertained by neuropathological lesions. AgNPs lowered serum
acetylcholine esterase, iron and copper levels, and increased creatine
phosphokinase and creatine phosphokinase–brain type activities. Following AgNPs
exposure, brain silver and iron contents were increased, but the copper level was
decreased. AgNPs up-regulated TNF-α (6.5-fold) and IL-1β (8.9-fold) transcript
levels, and simultaneously over-expressed the caspase-3 protein in cerebrum and
cerebellum inducing cell apoptosis. Moreover, AgNPs down-regulated the transcript
levels of tight-junction proteins; JP-1 (0.65-fold) and JAM-3(0.81-fold).
Significance: LA and relatively GB improved the serious effects of AgNPs on the
blood-brain barrier function and tight-junction proteins through their
antioxidants, anti-inflammatory, and anti-apoptotic efficacies. Co-treatment with
LA or GB may be favorable in ameliorating the neurotoxic side effects of AgNPs. ©
2018 Elsevier Inc.
AU - Lebda, M. A.
AU - Sadek, K. M.
AU - Tohamy, H. G.
AU - Abouzed, T. K.
AU - Shukry, M.
AU - Umezawa, M.
DB - Scopus
DO - 10.1016/j.lfs.2018.10.011
KW - Apoptosis
Blood-brain barrier
Ginkgo biloba L.
Neuroprotection
Neurotoxicity
Oxidative stress
Tight junctions
α-Lipoic acid
Animals
Antioxidants
Blood-Brain Barrier
Cells, Cultured
Cytokines
Ginkgo biloba
Lipid Peroxidation
Male
Metal Nanoparticles
Neurons
Oxidative Stress
Plant Extracts
Rats
Rats, Wistar
Silver
Thioctic Acid
acetylcholinesterase
caspase 3
catalase
copper ion
creatine kinase
Ginkgo biloba extract
glutathione
interleukin 1beta
iron
messenger RNA
silver nanoparticle
thioctic acid
tight junction protein
antioxidant
cytokine
metal nanoparticle
plant extract
silver
animal cell
animal experiment
animal tissue
Article
blood brain barrier
controlled study
copper blood level
down regulation
enzyme activity
enzyme blood level
histopathology
iron blood level
lipid peroxidation
male
neuroapoptosis
neuroprotection
nonhuman
oxidative stress
particle size
protein expression
protein RNA binding
rat
upregulation
animal
apoptosis
cell culture
chemistry
drug effect
metabolism
nerve cell
pathology
Wistar rat
M3 - Article
PY - 2018
SP - 251-260
ST - Potential role of α-lipoic acid and Ginkgo biloba against silver
rats
T2 - Life Sciences
TI - Potential role of α-lipoic acid and Ginkgo biloba against silver
rats
85054599359&doi=10.1016%2fj.lfs.2018.10.011&partnerID=40&md5=d7f6b294777beca386e349
e9fd360f2c
VL - 212
ID - 5375
ER -
TY - JOUR
AB - Staphylococcus aureus (S. aureus) forms biofilm that causes periprosthetic
joint infections and osteomyelitis (OM) which are the intractable health problems
in clinics. The silver-containing nanoparticles (AgNPs) are antibacterial
nanomaterials with less cytotoxicity than the classic Ag compounds. Likewise, gold
nanoparticles (AuNPs) have also been demonstrated as excellent nanomaterials for
medical applications. Previous studies have showed that both AgNPs and AuNPs have
anti-microbial or anti-inflammatory properties. We have developed a novel green
chemistry that could generate the AuAg nanocomposites, through the reduction of
tannic acid (TNA). The bioactivity of the nanocomposites was investigated in S.
aureus biofilm-exposed human osteoblast cells (hFOB1.19). The current synthesis
method is a simple, low-cost, eco-friendly, and green chemistry approach. Our
results showed that the AuAg nanocomposites were biocompatible with low cell
toxicity, and did not induce cell apoptosis nor necrosis in hFOB1.19 cells.
Moreover, AuAg nanocomposites could effectively inhibited the accumulation of
reactive oxygen species (ROS) in mitochondria and in rest of cellular compartments
after exposing to bacterial biofilm (by reducing 0.78, 0.77-fold in the cell and
mitochondria, respectively). AuAg nanocomposites also suppressed ROS-triggered
inflammatory protein expression via MAPKs and Akt pathways. The current data
suggest that AuAg nanocomposites have the potential to be a good therapeutic agent
in treating inflammation in bacteria-infected bone diseases. © 2023 IOP Publishing
Ltd.
AU - Lee, C. W.
AU - Lin, Z. C.
AU - Chiang, Y. C.
AU - Li, S. Y.
AU - Ciou, J. J.
AU - Liu, K. W.
AU - Lin, Y. C.
AU - Huang, B. J.
AU - Peng, K. T.
AU - Fang, M. L.
AU - Lin, T. E.
AU - Liao, M. Y.
AU - Lai, C. H.
C7 - 165101
DB - Scopus
DO - 10.1088/1361-6528/acb4a1
IS - 16
KW - AuAg nanocomposites
biofilm-induced diseases
eco-friendly green chemistry
inflammation
osteoblast
Bacteria
Biofilms
Gold
Humans
Inflammation
Metal Nanoparticles
Nanocomposites
Binary alloys
Biocompatibility
Cell death
Environmental protection
Gold nanoparticles
Mitochondria
Oxygen
Silver alloys
Silver compounds
antiinfective agent
gold
metal nanoparticle
nanocomposite
Auag nanocomposite
Biofilm-induced disease
Eco-friendly
Eco-friendly green chemistry
Green-chemistry
Human osteoblast
Mitochondrias
bacterium
biofilm
chemistry
human
metabolism
Sustainable chemistry
M3 - Article
PY - 2023
ST - AuAg nanocomposites suppress biofilm-induced inflammation in human
osteoblasts
T2 - Nanotechnology
TI - AuAg nanocomposites suppress biofilm-induced inflammation in human
osteoblasts
85147536288&doi=10.1088%2f1361-
6528%2facb4a1&partnerID=40&md5=602d89213d092daca9a0f6120ed463ae
VL - 34
ID - 4985
ER -
TY - JOUR
AB - Licoricidin, the fifth-highest fraction among the isolated 48 molecules from
Glycyrrhiza uralensis extracts, has been known as an anti-inflammatory bioactive
molecule; however, few studies have shown its inhibitory effect on T-cell
activation and atopic dermatitis (AD). This study examined the therapeutic
potential of licoricidin in AD by modulating T-cell activation with molecular
mechanisms. Licoricidin attenuated the expression of IL-2 mRNA in stimulated T
cells without cytotoxicity. Because tyrosine-protein phosphatase nonreceptor type 1
was predicted to interact physically with licoricidin in T cells in silico
analysis, the results of tyrosine-protein phosphatase nonreceptor type 1 activity
assay and phosphorylation study predicted that licoricidin might abrogate the
activity of tyrosine-protein phosphatase nonreceptor type 1 during T-cell
activation. Pretreatment with licoricidin controlled the dephosphorylation of Lck
on TCR-mediated stimulation. Moreover, licoricidin alleviated the symptoms of
dinitrochlorobenzene-and/or mite extract-induced AD, including ear thickness and
serum IgE level. Microscopic analysis also showed the effects of licoricidin on the
thickness of the dermis and epidermis and infiltration of immune cells.
Furthermore, mRNA levels of proinflammatory cytokines were attenuated in the ear
lesions of licoricidin-treated AD mice. Therefore, licoricidin has therapeutic
potential for treating AD, and its underlying mechanism involves effective
modulation of T-cell activation by controlling tyrosine-protein phosphatase
nonreceptor type 1 to maintain Lck phosphorylation.
AN - WOS:000703198200004
AU - Lee, H. S.
AU - Kim, J.
AU - Choi, H. G.
AU - Kim, E. K.
AU - Jun, C. D.
C6 - SEP 2021
DA - OCT
DO - 10.1016/j.jid.2021.02.759
IS - 10
PY - 2021
SN - 0022-202X
1523-1747
SP - 2490-+
ST - Licoricidin Abrogates T-Cell Activation by Modulating PTPN1 Activity and
Attenuates Atopic Dermatitis In Vivo
T2 - JOURNAL OF INVESTIGATIVE DERMATOLOGY
TI - Licoricidin Abrogates T-Cell Activation by Modulating PTPN1 Activity and
Attenuates Atopic Dermatitis In Vivo
VL - 141
ID - 6540
ER -
TY - JOUR
AB - The aim of this study was to investigate the impact of consuming dairy yogurt
supplemented with rhamnogalacturonan (RG), a polysaccharide from the peel of the
Korean citrus hallabong, on natural killer (NK) cell activity and circulating
cytokine levels. A randomized, double-blind, placebo-controlled study was conducted
on 120 nondiabetic and nonobese subjects. Over an eight-week period, the test group
consumed one pack (150 mL) of dairy yogurt containing 50 mg of probiotics and 100
mg of hallabong peel polysaccharide (60% RG) each day, whereas the placebo group
consumed the same product without the hallabong peel supplement. NK cell activity
(%) was measured based on the ratios of the effector cells (E; peripheral blood
mononuclear cells, PBMCs) from each participant relative to the target cells (T;
K562 cells) at E : T ratios of 10 : 1, 5 : 1, 2.5 : 1, or 1.25 : 1. NK cell
activities under all assay conditions and interleukin (IL)-12 and interferon (IFN)-
gamma levels were significantly increased in the test group at eight weeks compared
to the baseline values, whereas the placebo group showed a significant increase
only in NK cell activity at E : T = 1.25 : 1. The test group had significantly
greater increases in the changes in serum NK cell activity at the E : T ratios of
10 : 1, 5 : 1, and 2.5 : 1 and in the increases in IL-12 and IFN-gamma levels than
were observed in the placebo group, after adjusting for baseline values. After
eight weeks of treatment, significant reductions were found in IL-6 and IL-1 beta
levels in both the placebo and test groups. The daily consumption of dairy yogurt
supplemented with RG, a polysaccharide from the peel of the Korean citrus
hallabong, enhanced NK cell function and attenuated pro-inflammatory cytokine
levels.
AN - WOS:000378238100038
AU - Lee, M. H.
AU - Kim, M.
AU - Kwak, J. H.
AU - Chang, D. H.
AU - Yu, W. K.
AU - Lee, S. H.
AU - Lee, J. H.
DO - 10.1039/c5fo01103e
IS - 6
PY - 2016
SN - 2042-6496
2042-650X
SP - 2833-2839
ST - Consumption of dairy yogurt with the polysaccharide rhamnogalacturonan from
the peel of the Korean citrus hallabong enhances immune function and attenuates the
T2 - FOOD & FUNCTION
TI - Consumption of dairy yogurt with the polysaccharide rhamnogalacturonan from
the peel of the Korean citrus hallabong enhances immune function and attenuates the
VL - 7
ID - 6688
ER -
TY - JOUR
AB - In this study, the potential of cherry silver belly (Elaeagnus multiflora) as
a cancer preventive agent through regulating inflammatory signals including
cyclooxygenase-2 (COX-2) and Akt was examined. Cherry silver berry has reported to
exert anti-oxidative, anti-inflammatory, and anti-proliferative effects. The
extracts from seed and flesh of cherry silver berry were obtained, and analyzed
COX-2 and Akt activities in cherry silver berry extract treated HT-29 colon cancer
cells. The results showed that the treatment of seed extracts reduced cell
viability at the concentrations above 1,600 mg/mL, effectively reduced COX-2 and p-
Akt expression. In addition, the anti-inflammatory effects seemed to be related to
cancer cell death. Both of seed and flesh extracts inhibited cell growth and
induced apoptosis in HT-29 cells. These results suggest that extracts of cherry
silver berry may contribute to suppressing cancer growth through its anti-
inflammatory and anti-proliferative effects, and natural products used as oriental
medicine have the possibility to control tumor cell growth.
AN - WOS:000285906100036
AU - Lee, M. S.
AU - Lee, Y. K.
AU - Park, O. J.
DA - DEC
DO - 10.1007/s10068-010-0237-1
IS - 6
PY - 2010
SN - 1226-7708
2092-6456
SP - 1673-1677
ST - Cherry Silver Berry (Elaeagnus multiflora) Extracts Exert Anti-inflammatory
Effects by Inhibiting COX-2 and Akt Signals in HT-29 Colon Cancer Cells
T2 - FOOD SCIENCE AND BIOTECHNOLOGY
TI - Cherry Silver Berry (Elaeagnus multiflora) Extracts Exert Anti-inflammatory
Effects by Inhibiting COX-2 and Akt Signals in HT-29 Colon Cancer Cells
VL - 19
ID - 5993
ER -
TY - JOUR
AB - In this study the potential of cherry silver berry (Elaeagnus multiflora) as
a cancer preventive agent through regulating inflammatory signals including
cyclooxygenase- 2 (COX-2) and Akt was examined. Cherry silver berry has reported to
exert anti-oxidative anti-inflammatory and antiproliferative effects. The extracts
from seed and flesh of cherry silver berry were obtained and analyzed COX-2 and Akt
activities in cherry silver berry extract treated HT- 29 colon cancer cells. The
results showed that the treatment of seed extracts reduced cell viability at the
concentrations above 1,600 mg/mL effectively reduced COX-2 and p-Akt expression. In
addition the antiinflammatory effects seemed to be related to cancer cell death.
Both of seed and flesh extracts inhibited cell growth and induced apoptosis in HT-
29 cells. These results suggest that extracts of cherry silver berry may contribute
to suppressing cancer growth through its anti-inflammatory and anti-proliferative
effects and natural products used as oriental medicine have the possibility to
control tumor cell growth. © KoSFoST and Springer 2011.
AU - Lee, M. S.
AU - Lee, Y. K.
AU - Park, O. J.
DB - Scopus
DO - 10.1007/s10068-010-0237-1
IS - 6
KW - Akt
Apoptosis
Colon cancer
Cyclooxygenase-2
Elaeagnus multiflora
Cell death
Cell growth
Diseases
Fruits
Growth kinetics
Seed
Anti-inflammatories
Antiproliferative effect
Cancer cells
Cancer growth
Cell viability
Colon cancer cells
Cyclooxygenase 2
Cyclooxygenases
Induced apoptosis
Natural products
Oriental medicines
Preventive agents
Silver
M3 - Article
PY - 2010
SP - 1673-1677
ST - Cherry silver berry (Elaeagnus multiflora) extracts exert antiinflammatory
effects by inhibiting COX-2 and Akt signals in HT-29 colon cancer cells
T2 - Food Science and Biotechnology
TI - Cherry silver berry (Elaeagnus multiflora) extracts exert antiinflammatory
effects by inhibiting COX-2 and Akt signals in HT-29 colon cancer cells
79959737326&doi=10.1007%2fs10068-010-0237-
1&partnerID=40&md5=564756635df35f2d93600b8fde067da9
VL - 19
ID - 5710
ER -
TY - JOUR
AB - Alzheimer disease (AD) is a neurodegenerative disorder characterized by
excessive accumulation of amyloid-beta peptide (A) and progressive loss of neurons.
Therefore, the inhibition of A-induced neurotoxicity is a potential therapeutic
approach for the treatment of AD. Ecklonia cava is an edible brown seaweed, which
has been recognized as a rich source of bioactive derivatives, mainly
phlorotannins. In this study, phlorotannins including eckol, dieckol, 8,8-bieckol
were used as potential neuroprotective candidates for their anti-apoptotic and
anti-inflammatory effects against A(25-35)-induced damage in PC12 cells. Among the
tested compounds, dieckol showed the highest effect in both suppressing
intracellular oxidative stress and mitochondrial dysfunction and activation of
caspase family. Three phlorotannins were found to inhibit TNF-, IL-1 and PGE(2)
production at the protein levels. These result showed that the anti-inflammatory
properties of our compounds are related to the down-regulation of proinflammatory
enzymes, iNOS and COX-2, through the negative regulation of the NF-B pathway in
A(25-35)-stimulated PC12 cells. Especially, dieckol showed the strong anti-
inflammatory effects via suppression of p38, ERK and JNK. However, 8,8-bieckol
markedly decreased the phosphorylation of p38 and JNK and eckol suppressed the
activation of p38. Therefore, the results of this study indicated that dieckol from
E. cava might be applied as a drug candidate for the development of new generation
therapeutic agents against AD.
AN - WOS:000458053200007
AU - Lee, S.
AU - Youn, K.
AU - Kim, D. H.
AU - Ahn, M. R.
AU - Yoon, E.
AU - Kim, O. Y.
AU - Jun, M.
C7 - 7
DA - JAN
DO - 10.3390/md17010007
IS - 1
PY - 2019
SN - 1660-3397
ST - Anti-Neuroinflammatory Property of Phlorotannins from Ecklonia cava on A(25-
35)-Induced Damage in PC12 Cells
T2 - MARINE DRUGS
TI - Anti-Neuroinflammatory Property of Phlorotannins from Ecklonia cava on A(25-
35)-Induced Damage in PC12 Cells
VL - 17
ID - 6443
ER -
TY - JOUR
AB - Background: Deposition and accumulation of silver nanoparticles (Ag-nps) in
the liver have been shown to induce hepatotoxicity in animal studies. The
hepatotoxicity may include oxidative stress, abnormalities in energy metabolism,
and cell death. Studies have indicated that autophagy is an intracellular event
involving balance of energy, nutrients, and turnover of subcellular organelles. The
present study was undertaken to test the hypothesis that autophagy plays a role in
mediating hepatotoxicity in animal after exposure to Ag-nps. Focus was placed on
interrelationship between energy metabolism, autophagy, apoptosis and hepatic
dysfunction.Methods: Sprague Dawley rats were intraperitoneally injected with Ag-
nps (10-30 nm in diameter) at concentration of 500 mg kg-1. All animals were
sacrificed on days 1, 4, 7, 10 and 30 after exposure and blood and liver tissues
were collected for further studies.Results: Uptake of Ag-nps was quite prompt and
not proportional to the blood Ag concentration. Declination of ATP (-64% in days 1)
and autophagy (determined by LC3-II protein expression and morphological
evaluation) increased and peaked on the first day. The ATP content remained at low
level even though the autophagy has been activated. Apoptosis (based on caspase-3
protein expression and TUNEL-positive cells staining) began to rise sigmoidally at
days 1 and 4, reached a peak level at day 7, and remained at the same levels during
days 7-30 post exposure. Meanwhile, autophagy exhibited a gradual decrease from
days 1-10 and the decrease at day 30 was statistically significant as compared to
day 0 (sham group). Inflammatory reaction (histopathological evaluation) was found
at day 10 and preceded to an advanced degree at day 30 when liver function was
impaired.Conclusions: These results indicate that following Ag-nps administration,
autophagy was induced; however, failure to preserve autophagy compounded with
energy reduction led to apoptosis and the eventual impairment of liver function.
The study provides an in-vivo evidence of hepatotoxicity by continuous exposure of
Ag-nps in rats. © 2013 Lee et al.; licensee BioMed Central Ltd.
AU - Lee, T. Y.
AU - Liu, M. S.
AU - Huang, L. J.
AU - Lue, S. I.
AU - Lin, L. C.
AU - Kwan, A. L.
AU - Yang, R. C.
C7 - 40
DB - Scopus
DO - 10.1186/1743-8977-10-40
IS - 1
KW - Apoptosis
ATP
Autophagy
Animals
Caspase 3
Drug-Induced Liver Injury
Energy Metabolism
Injections, Intraperitoneal
Liver
Male
Microtubule-Associated Proteins
Nanoparticles
Silver
Time Factors
caspase 3
LC3 II protein
protein
silver nanoparticle
unclassified drug
Casp3 protein, rat
LC3 protein, rat
microtubule associated protein
nanoparticle
silver
animal experiment
animal model
animal tissue
apoptosis
article
autophagy
bioenergy
blood level
cell structure
controlled study
energy metabolism
exposure
histopathology
in vivo study
inflammation
liver dysfunction
liver level
liver toxicity
male
nick end labeling
nonhuman
priority journal
protein expression
rat
Sprague Dawley rat
animal
blood
drug effects
liver
liver function test
metabolism
pathology
time
ultrastructure
M3 - Article
PY - 2013
ST - Bioenergetic failure correlates with autophagy and apoptosis in rat liver
following silver nanoparticle intraperitoneal administration
TI - Bioenergetic failure correlates with autophagy and apoptosis in rat liver
following silver nanoparticle intraperitoneal administration
84884316196&doi=10.1186%2f1743-8977-10-
40&partnerID=40&md5=27938206537a473f0f13d9036c0d8f9d
VL - 10
ID - 5720
ER -
TY - JOUR
AB - Clinical management of Clostridium difficile infection is still far from
satisfactory as bacterial spores are resistant to many chemical agents and physical
treatments. Certain types of nanoparticles have been demonstrated to exhibit anti-
microbial efficacy even in multi-drug resistance bacteria. However, most of these
studies failed to show biocompatibility to the mammalian host cells and no study
has revealed in vivo efficacy in C. difficile infection animal models. The spores
treated with 500 mu g/mL Fe3-delta O4 nanoparticles for 20 minutes, 64% of the
spores were inhibited from transforming into vegetative cells, which was close to
the results of the sodium hypochlorite-treated positive control. By cryo-electron
micro-tomography, we demonstrated that Fe3-delta O4 nanoparticles bind on spore
surfaces and reduce the dipicolinic acid (DPA) released by the spores. In a C.
difficile infection animal model, the inflammatory level triple decreased in mice
with colonic C. difficile spores treated with Fe3-delta O4 nanoparticles.
Histopathological analysis showed a decreased intense neutrophil accumulation in
the colon tissue of the Fe3-delta O4 nanoparticle-treated mice. Fe3-delta O4
nanoparticles, which had no influence on gut microbiota and apparent side effects
in vivo, were efficacious inhibitors of C. difficile spore germination by attacking
its surface and might become clinically feasible for prophylaxis and therapy.
AN - WOS:000407570000001
AU - Lee, W. T.
AU - Wu, Y. N.
AU - Chen, Y. H.
AU - Wu, S. R.
AU - Shih, T. M.
AU - Li, T. J.
AU - Yang, L. X.
AU - Yeh, C. S.
AU - Tsai, P. J.
AU - Shieh, D. B.
C7 - 8124
DA - AUG 15
DO - 10.1038/s41598-017-08387-y
PY - 2017
SN - 2045-2322
ST - Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore
Germination and Attenuated Local and Systemic Inflammation
TI - Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore
Germination and Attenuated Local and Systemic Inflammation
VL - 7
ID - 6706
ER -
TY - JOUR
AB - The biotoxicity caused by focus releasing of Ag, which associated with the Ag
loading mode, is a problematic issue that need to be solved for practical
utilization of the keratin based wound dressing. In this study, keratin/AgNPs blend
scaffolds (Ker/Ag) and keratin scaffolds with AgNPs attached on the scaffold's wall
surface (Ag@Ker) were prepared. Structure and physical properties of the scaffolds
were tested and investigated. In comparison to the Ag@Ker scaffolds, the Ker/Ag
scaffolds with uniform dispersion of AgNPs have larger tensile strength and slower
degradation rate. Both kind of scaffolds present excellent antibacterial property
with 10 mu g mL(-1) AgNPs addition, while the Ker/Ag displayed a linear Ag
releasing ratio in the first 5-7 days, which is beneficial for obtaining a
continuous antibacterial property and avoiding the biotoxicity caused by focus
release of Ag. Correspondingly, cytotoxicity assay further reveals that the
continuously slow release of Ag of the Ker/Ag scaffolds accelerated the
proliferation of cell. Infectious animal models and histological studies showed
that the Ker/Ag scaffolds can effectively inhibit the inflammatory response and
accelerate epithelialization. Thus, it can be concluded that the Ker/Ag scaffolds
with uniform dispersion of AgNPs are more attractive as wound repair materials.
AN - WOS:000908088600001
AU - Lei, T. D.
AU - Fan, J.
AU - Wang, Y. H.
AU - Cao, F. Y.
AU - Yang, Q. Q.
AU - Tian, F. M.
AU - Li, B.
AU - Su, Z. B.
AU - Chen, R. X.
AU - Liu, Y.
C6 - JAN 2023
DA - JAN
DO - 10.1177/08853282221150685
IS - 6
PY - 2023
SN - 0885-3282
1530-8022
SP - 1071-1085
ST - The fabrication and evaluation of silver nanoparticle-based keratin scaffolds
T2 - JOURNAL OF BIOMATERIALS APPLICATIONS
TI - The fabrication and evaluation of silver nanoparticle-based keratin scaffolds
VL - 37
ID - 6392
ER -
TY - JOUR
AB - The biotoxicity caused by focus releasing of Ag, which associated with the Ag
loading mode, is a problematic issue that need to be solved for practical
utilization of the keratin based wound dressing. In this study, keratin/AgNPs blend
scaffolds (Ker/Ag) and keratin scaffolds with AgNPs attached on the scaffold’s wall
surface (Ag@Ker) were prepared. Structure and physical properties of the scaffolds
were tested and investigated. In comparison to the Ag@Ker scaffolds, the Ker/Ag
scaffolds with uniform dispersion of AgNPs have larger tensile strength and slower
degradation rate. Both kind of scaffolds present excellent antibacterial property
with 10 μg mL−1 AgNPs addition, while the Ker/Ag displayed a linear Ag releasing
ratio in the first 5–7 days, which is beneficial for obtaining a continuous
antibacterial property and avoiding the biotoxicity caused by focus release of Ag.
Correspondingly, cytotoxicity assay further reveals that the continuously slow
release of Ag of the Ker/Ag scaffolds accelerated the proliferation of cell.
Infectious animal models and histological studies showed that the Ker/Ag scaffolds
can effectively inhibit the inflammatory response and accelerate epithelialization.
Thus, it can be concluded that the Ker/Ag scaffolds with uniform dispersion of
AgNPs are more attractive as wound repair materials. © The Author(s) 2023.
AU - Lei, T.
AU - Fan, J.
AU - Wang, Y.
AU - Cao, F.
AU - Yang, Q.
AU - Tian, F.
AU - Li, B.
AU - Su, Z.
AU - Chen, R.
AU - Liu, Y.
DB - Scopus
DO - 10.1177/08853282221150685
IS - 6
biocompatibility
drug release
loading strategy
wound dressing
Animals
Keratins
Metal Nanoparticles
Silver
Tissue Scaffolds
Degradation
Dispersions
Keratin
Scaffolds (biology)
Tensile strength
keratin
silver nanoparticle
antiinfective agent
metal nanoparticle
silver
Ag loadings
Biotoxicity
Drug release
Loading modes
Loading strategy
Problematic issues
Uniform dispersions
Wound dressings
animal cell
Article
cell proliferation
cell viability
controlled study
degradation
dispersion
drug effect
drug efficacy
drug mechanism
drug response
electron microscopy
Escherichia coli
mouse
nanofabrication
NCTC clone 929 cell line
nonhuman
physical chemistry
surface property
tensile strength
thermogravimetry
animal
chemistry
tissue scaffold
Biocompatibility
M3 - Article
PY - 2023
SP - 1071-1085
ST - The fabrication and evaluation of silver nanoparticle-based keratin scaffolds
T2 - Journal of Biomaterials Applications
TI - The fabrication and evaluation of silver nanoparticle-based keratin scaffolds
85145923002&doi=10.1177%2f08853282221150685&partnerID=40&md5=ab6382aecf81a7bfd3cfcb
7e6d09269d
VL - 37
ID - 5049
ER -
TY - JOUR
AB - Transgenic mice expressing a mutated human Cu/Zn superoxide dismutase (SOD1)
gene develop a motor neuron disease similar to familial amyotrophic lateral
sclerosis (FALS). While the histopathology and the inflammatory reactions in the
spinal cord of these mice are well described, their spatiotemporal extension into
brain areas and the relationship between degenerative and inflammatory events
remain obscure. In the present study, we investigated the time course and extent of
degenerative changes and inflammatory reactions in the CNS during progression of
the disease in a transgenic FALS model, the SOD1-G93A mouse with histological and
immunohistochemical methods. Compared to non-transgenic littermates, the SOD1-G93A
transgenics developed widespread degeneration in both motor and extra-motor regions
up to telencephalic regions, including the cerebral cortex but sparing distinct
regions like the striatum and hippocampus. We provide evidence that these
degenerative processes are accompanied by intense inflammatory reactions in the
brain, which spatiotemporally correlate with degeneration and comprise besides
strong astro- and microgliotic reactions also an influx of peripheral immune cells
such as T-lymphocytes and dendritic cells. Both degeneration and inflammatory
reactions spread caudocranially, starting at 2 months in the spinal cord and
reaching the telencephalon at 5 months of age. Since the corticospinal tract lacked
any signs of degeneration, we conclude that the upper and the lower motor neurons
degenerate independently of each other. (c) 2006 Elsevier B.V. All rights reserved.
AN - WOS:000239068200019
AU - Leichsenring, A.
AU - Linnartz, B.
AU - Zhu, X. R.
AU - Lubbert, H.
AU - Stichel, C. C.
DA - JUN 22
DO - 10.1016/j.brainres.2006.04.029
PY - 2006
SN - 0006-8993
1872-6240
SP - 180-195
ST - Ascending neuropathology in the CNS of a mutant SOD1 mouse model of
T2 - BRAIN RESEARCH
TI - Ascending neuropathology in the CNS of a mutant SOD1 mouse model of
VL - 1096
ID - 6619
ER -
TY - JOUR
AB - Despite studies concerning toxic reactions related to amalgam components in
the literature, few studies have been devoted to evaluate local noxious effects of
amalgam tattoos (AT) on biological tissues. In addition, little is known about
activation of inflammatory cells by mucosa-implanted amalgam debris. Tissue
reaction to AT depends on the particle size. Human leukocyte antigen DR (HLA-DR) is
an activation marker of inflammatory cells associated with antigen presentation.
Metallothioneins (MT) are proteins involved with metal detoxication, including
mercury and silver. The purpose of the present study was to investigate the
immunolocalization of HLA-DR and MT in AT with large or powdered particles.
Paraffin-embedded AT tissue blocks were sectioned and subjected to
immunohistochemistry for HLA-DR and MT localization. The results demonstrated a
dense mononuclear inflammatory infiltrate associated with large and powdered debris
and positivity for HLA-DR and MT in inflammatory cells. While blood vessel walls
and connective fibers impregnated with powdered particles were negative for HLA-DR,
they were positive for MT. In addition, wherever epithelial basement membrane
impregnation by powdered amalgam particles was observed, a strong positivity for MT
was detected. These findings demonstrate that residual elements of AT still have
noxious local effects over tissues.
AU - Leite, C. M. A.
AU - Botelho, A. S.
AU - Oliveira, J. R.
AU - Cardoso, S. V.
AU - Loyola, A. M.
AU - Gomez, R. S.
AU - Vaz, R. R.
DB - Scopus
DO - 10.1590/s0103-64402004000200003
IS - 2
KW - Amalgam tattoo
Dental amalgam
HLA-DR
Metallothionein
Toxicity
Argyria
Dental Amalgam
HLA-DR Antigens
Humans
Immunoenzyme Techniques
Mouth Mucosa
Particle Size
Pigmentation Disorders
dental alloy
HLA DR antigen
leukocyte antigen
mercury
metallothionein
paraffin
silver
antigen presentation
article
basement membrane
blood vessel wall
controlled study
detoxification
human
human cell
immunolocalization
inflammatory cell
particle size
tissue reaction
argyria
chemistry
enzyme immunoassay
immunology
metabolism
mononuclear cell
mouth mucosa
pigment disorder
M3 - Article
PY - 2004
SP - 99-103
ST - Immunolocalization of HLA-DR and metallothionein on amalgam tattoos
T2 - Brazilian Dental Journal
TI - Immunolocalization of HLA-DR and metallothionein on amalgam tattoos
15544371972&doi=10.1590%2fs0103-
64402004000200003&partnerID=40&md5=c04407ca1c7d9bdb5cb8f862481d5eca
VL - 15
ID - 5780
ER -
TY - JOUR
AB - Metals are components of a variety of biomaterials used in orthopedic and
dental appliances; however, their biocompatibility with the surrounding tissues is
not completely understood. Monocytes are important immune cells that respond to
inflammatory stimuli by rapidly producing a variety of inflammatory proteins.
Regulation of this response often involves activation of the transcription factor
NFκB. The current study was designed to determine whether monocyte activation of
NFκB in response to bacterial lipopolysaccharide (LPS) is affected by pretreatment
with metal ions. Concentrations of metal ions that affected cell number after 24 h
of exposure were first determined. Then THP-1 human monocytes were cultured for 2 h
in media containing metal ions at concentrations below levels that altered cell
growth. Parallel cultures were treated with 10 μg/mL Escherichia coli LPS, and all
samples were cultured an additional 2 h. Nuclear proteins were extracted and
normalized amounts were incubated with [32p]-end-labeled NFκB consensus
oligonucleotide. NFκB-DNA complexes were identified and quantified by
electrophoretic mobility shift analysis. The extent of NFκB-DNA complex formation
after metal ion pretreatment with or without LPS induction was compared to no
treatment or LPS-only treated controls. Finally, LPS-induced IL1β secretion was
measured from palladium-treated and control cells. Concentrations were identified
for each metal ion (Ag+, Co 2+ Cu2+, Hg2+, Ni2+, and Pd 2+) that did not reduce
cell number after 24 h of exposure (ranging from 5 μM for Ag+ and Hg2+ to 200 μM
for Ni 2+). Exposures of 2 h at these concentrations did not alter cell morphology,
staining with trypan blue, or cell number. LPS exposure had no effect on cell
number with or without metal ions after 2 h. When metal treatment alone was
assessed, none of the metal ions had a significant effect on NFκB-DNA binding.
However, pretreatment with Co2+, Ni 2+, Ag1+, Hg2+, and Pd2+ significantly
decreased NFκB-DNA binding by 40-70% versus LPS alone. Only Cu2+ had no effect on
LPS-induced NFκB-DNA complex formation. Pd2+ lowered, but did not abolish, IL1β
secretion at concentrations comparable to those that altered NFκB-DNA binding.
These results suggest that many commonly used metals alter monocyte function at
concentrations that are not overtly toxic, and that protein levels controlled in
part by NFκB also may be altered. © 2003 Wiley Periodicals, Inc.
AU - Lewis, J. B.
AU - Wataha, J. C.
AU - Randol, T. M.
AU - McCloud, V. V.
AU - Lockwood, P. E.
DB - Scopus
DO - 10.1002/jbm.a.10169
IS - 3
KW - Dental materials
Heavy metals
Inflammation
NFκB
Biocompatibility
Biomaterials
Cell culture
Cells
Flammability
Heavy ions
Immunology
biomaterial
cobalt
copper ion
heavy metal
interleukin 1beta
lipopolysaccharide
mercury
metal ion
nickel
nuclear protein
oligonucleotide
palladium
phosphorus 32
protein
silver
trypan blue
Metal ions
article
biocompatibility
bone prosthesis
cell activation
cell count
cell function
cell growth
cell structure
controlled study
cytokine release
DNA protein complex
Escherichia coli
gel mobility shift assay
human
human cell
inflammation
monocyte
tooth filling
Polysaccharides
M3 - Article
PY - 2003
SP - 868-875
ST - Metal ions alter lipopolysaccharide-induced NFκB binding in monocytes
TI - Metal ions alter lipopolysaccharide-induced NFκB binding in monocytes
0346754910&doi=10.1002%2fjbm.a.10169&partnerID=40&md5=f0542b5b6ce3633f0ef1b747f0876
243
VL - 67
ID - 5764
ER -
TY - JOUR
AB - Aim: White tip silver needle, a slightly fermented white tea, is abundant in
flavonoids, and it has great significance in terms of
D-galactose/lipopolysaccharide-induced aging in mice. Methods: We analyzed the
antioxidant capacity of white tip silver needle flavonoids (WTSNF) in vitro,
assessed the effects of WTSNF on organ indexes, pathological changes, liver
function indexes, biochemical indicators, molecular biological indicators, and
genes related to oxidation and inflammation. Results: Ultra-high performance liquid
chromatography-tandem mass spectrometry results showed that WTSNF contained
baicalin, kaempferol, kaempferide, quercetin, isorhamnetin, lespenephryl, and
rutin. WTSNF showed strong scavenging ability for both 1,1-diphenyl-2-
picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)
diammonium salt (ABTS) free radicals. Pathological analysis results showed that
WTSNF reduced liver, kidney, and lung damage in mice with induced aging. In the
serum and liver tissue, WTSNF effectively increased the antioxidant-related levels
of superoxide dismutase, catalase, glutathione peroxidase, glutathione, and total
antioxidant capacity and reduced the levels of aspartate aminotransferase, alanine
aminotransferase, malondialdehyde and nitric oxide. WTSNF also reduced the
inflammation-related levels of interleukin-6, interleukin-1 beta, tumor necrosis
factor alpha (TNF alpha), and interferon gamma (IFN-gamma) and increased the levels
of interleukin-10 and interleukin-12. Furthermore, WTSNF upregulated the mRNA
expression levels of cupro-zinc superoxide dismutase, manganese superoxide
dismutase, catalase, glutathione peroxidase, interleukin-10, neuronal nitric oxide
synthase, endothelial nitric oxide synthase, nuclear factor erythroid 2-related
factor, heme oxygenase 1, NAD(P)H dehydrogenase [quinone] 1, nuclear factor of
kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (I kappa B-
alpha), and thioredoxin, while it downregulated the mRNA expression levels of
interleukin-6, interleukin-18, interleukin-1 beta, TNF alpha, IFN-gamma, inducible
nitric oxide synthase, cyclooxygenase-2, and nuclear factor kappa-light chain-
enhancer of activated B cells (NF-kappa B). Conclusion: WTSNF is a high-quality
natural product with antioxidative and anti-inflammatory properties that can
inhibits D-galactose/lipopolysaccharide-induced aging in mice.
AN - WOS:000640061800001
AU - Li, C.
AU - He, J. C.
AU - Yang, Y.
AU - Gou, Y. T.
AU - Wang, Z. Y.
AU - Chen, H.
AU - Zhao, X.
DO - 10.2147/DDDT.S304885
PY - 2021
SN - 1177-8881
SP - 1441-1457
ST - White Tip Silver Needle (Slightly Fermented White Tea) Flavonoids Help
Prevent Aging via Antioxidative and Anti-Inflammatory Effects
T2 - DRUG DESIGN DEVELOPMENT AND THERAPY
TI - White Tip Silver Needle (Slightly Fermented White Tea) Flavonoids Help
Prevent Aging via Antioxidative and Anti-Inflammatory Effects
VL - 15
ID - 6121
ER -
TY - JOUR
AB - Geniposide (GE) can effectively inhibit diabetic nephropathy (DN), but its
mechanism is unclear. The objective of this study was to explore the antidiabetic
nephropathy effects of GE both in high fat diet/streptozotocin-induced DN mice and
in high glucose-induced podocyte model. Renal function in DN mice was evaluated by
levels of serum creatinine (Scr) and blood urea nitrogen (BUN). Renal inflammation
was appraised by pro-inflammatory cytokines: Tumor necrosis factor α (TNF-α),
Interleukin 6 (IL-6) and IL-1β via ELISA assay. Renal histopathology analysis was
conducted via hematoxylin and eosin, Masson and periodic acid-silver metheramine
staining. Cellular viability was measured by Terminal deoxynucleotidyl transferase-
mediated dUTP-biotin nick end labeling assay. Moreover, the related proteins p–NF–
κB, ASC, Cleave-IL-1β, NLRP3, Cleave-Caspase-1 and GSDMD-N in AMPK/SIRT1/NF-κB
pathway were assayed by Western blotting. In order to further investigate the
effects of GE on podocytes, we also assessed these protein levels in AMPK/SIRT1/NF-
κB pathway after siRNA-AMPK intervention by Western blotting. GE alleviated renal
dysfunction as evidenced by decreased levels of Scr, BUN, TNF-α, IL-6 and IL-1β.
Histological examination revealed GE effectively attenuated kidney damage,
including glomerular basement membrane thickening and inflammatory cells
infiltration. AMPK, p-AMPK and SIRT1 levels were obviously decreased both in DN
mice and in podocyte model, but GE reversed these changes. The protein expressions
in APMK/SIRT1/NF-κB pathway were significantly decreased by GE treatment. These
results suggested that GE could efficiently block oxidative stress and inflammatory
responses accompanied with pyroptosis, thus inhibiting the development of DN, and
its mechanism might be related to APMK/SIRT1/NF-κB pathway. © 2020 Elsevier B.V.
AU - Li, F.
AU - Chen, Y.
AU - Li, Y.
AU - Huang, M.
AU - Zhao, W.
C7 - 173449
DB - Scopus
DO - 10.1016/j.ejphar.2020.173449
KW - APMK/SIRT1/NF-κB pathway
Diabetic nephropathy
Geniposide
Oxidative stress
AMP-Activated Protein Kinases
Animals
Apoptosis
Blood Urea Nitrogen
Creatinine
Cytokines
Diabetic Nephropathies
Diet, High-Fat
Iridoids
Kidney
Male
Mice
Mice, Inbred C57BL
NF-kappa B
Podocytes
RNA, Small Interfering
Signal Transduction
Sirtuin 1
creatinine
cryopyrin
geniposide
hydroxymethylglutaryl coenzyme A reductase kinase
interleukin 1beta
interleukin 6
malonaldehyde
nitrogen
sirtuin 1
urea
valsartan
cytokine
iridoid
Sirt1 protein, mouse
AMPK signaling
AMPK SIRT1 NF kappa B pathway
animal cell
animal experiment
animal model
animal tissue
Article
controlled study
drug dose comparison
glomerulus basement membrane
in vitro study
in vivo study
male
mouse
nonhuman
podocyte
priority journal
pyroptosis
signal transduction
animal
apoptosis
blood
C57BL mouse
complication
drug effect
kidney
lipid diet
metabolism
pathology
M3 - Article
PY - 2020
ST - Geniposide alleviates diabetic nephropathy of mice through AMPK/SIRT1/NF-κB
pathway
T2 - European Journal of Pharmacology
TI - Geniposide alleviates diabetic nephropathy of mice through AMPK/SIRT1/NF-κB
pathway
85089495271&doi=10.1016%2fj.ejphar.2020.173449&partnerID=40&md5=6c38dd67d439aa3def6
c4b9212383b5c
VL - 886
ID - 5275
ER -
TY - JOUR
AB - BACKGROUND: An ideal repairing material characterizes by both great
biocompatibility and osteogenesis ability. Any biomaterials should meet excellent
biological security and biocompatibility prior to clinical application. OBJECTIVE:
To evaluate the biocompatibility and the biological security of the TiO2-Ag-nano-
hydroxyapatite/polyamide66 (TiO 2-Ag-nHA/PA66) composite. DESIGN, TIME AND SETTING:
A randomized controlled duplicated design was performed in Chongqing Medical
University from July 2008 to July 2009. MATERIALS: A total of 40 Kunming mice aged
3 weeks and of clean grade and 32 healthy adult New Zealand rabbits were provided
by Experimental Animal Center of Chongqing Medical University. TiO 2-Ag-nHA/PA66
composite powder (10 g), TiO2-Ag-nHA/PA66 composites (n=32, 5 mm × 25 mm), and
TiO2-Ag-nHA/PA66 composites (n=32, 3 mm × 5 mm) were provided by Research Center of
Nano-Biomaterials of Sichuan University. METHODS: General toxicity test: Forty mice
were randomly divided into experimental and control groups. Composite powder was
used to prepare leaching liquor which was treated on experimental mice by an
intraperitoneal injection. While, an equal amount saline was inserted into the
control mice. Intramuscular implanting test: The 16 rabbits were randomly divided
into experimental and control groups. Two TiO 2-Ag-nHA/PA66 composite (5 mm × 25
mm) were implanted into left and right erector spinae, respectively. A similar
surgery with the exception of implantation was performed on the control rabbits.
Intrabony implanting test: The resting 16 rabbits were implanted one
TiO2-Ag-nHA/PA66 composite (3 mm × 5 mm) into left and right lateral epicondyle,
respectively. Hemolytic test: A 8-mL anti-coagulation blood was obtained and added
with composite powder (0.1, 0.15, and 0.2 g). MAIN OUTCOME MEASURES:
Biocompatibility and biological security of TiO2-Ag-nHA/PA66 composite. RESULTS:
General toxicity test demonstrated that mice in the two groups had good activity
and eating, normal breathing, stable body mass, no paralysis, convulsion, and
death. Intramuscular implanting test and intrabony implanting test showed that
there was no significant difference in levels of alanine aminotransferase,
aspartate transaminase, urea nitrogen, and creatinine and leukocyte numbers between
the two groups before implantation and at day 4, 1 week, and 2 weeks after
implantation (P > 0.05). In particular, there was no significant difference in the
experimental group at varying time points (P > 0.05). Intramuscular implanting test
indicated that tissue sections in the experimental group displayed coated tissue of
materials. The inflammatory variation in the experimental group was generally
similar to that in the control group. Intrabony implanting test indicated that
tissue sections in the experimental group displayed new bone formation. Hemolytic
test suggested that hemolytic ratio of three varying concentrations of TiO2-Ag-
nHA/PA66 composites was less than 5%, which met the normal criterion. CONCLUSION:
TiO2-Ag-nHAPA66 composite has a good biocompatibility and biological security.
AU - Li, G. Z.
AU - Jiang, D. M.
AU - Tan, Z. J.
AU - Lu, M. P.
AU - Kuang, S. R.
AU - Peng, C.
AU - Guo, Z. P.
DB - Scopus
DO - 10.3969/j.issn.1673-8225.2009.47.005
IS - 47
KW - Animalia
Mus
polyamide 66
titanium dioxide
titanium dioxide silver nano hydroxyapatite
unclassified drug
aged
article
biocompatibility
breathing
convulsion
death
eating
mouse
nonhuman
rabbit
tissue section
toxicity testing
M3 - Article
PY - 2009
SP - 9231-9235
ST - Biocompatibility and biological security of the TiO2-Ag-nano-
hydroxyapatite/polyamide66 composite
T2 - Journal of Clinical Rehabilitative Tissue Engineering Research
TI - Biocompatibility and biological security of the TiO2-Ag-nano-
hydroxyapatite/polyamide66 composite
77953717248&doi=10.3969%2fj.issn.1673-
8225.2009.47.005&partnerID=40&md5=b4d7ccc43266229a1181e7566401f3f5
VL - 13
ID - 5737
ER -
TY - JOUR
AB - With the increasing applications of silver nanoparticles (Ag NPs), the
concerns of widespread human exposure as well as subsequent health risks have been
continuously growing. The acute and chronic toxicities of Ag NPs in cellular tests
and animal tests have been widely investigated. Accumulating evidence shows that Ag
NPs can induce inflammation, yet the overall mechanism is incomplete. Herein, using
gold nanorod core/silver shell nanostructures (Au@Ag NRs) as a model system, we
studied the influence on mice liver and lungs from the viewpoint of metabolism. In
agreement with previous studies, Au@Ag NRs' intravenous exposure caused
inflammatory reaction, accompanying with metabolic alterations, including energy
metabolism, membrane/choline metabolism, redox metabolism, and purine metabolism,
the disturbances of which contribute to inflammation. At the same time, dopamine
metabolism in liver was also changed. This is the first time to observe the
production of dopamine in non-neural tissue after treatment with Ag NPs. As the
upregulation of dopamine resists inflammation, it indicates the activation of
antioxidant defense systems against oxidative stress induced by Au@Ag NRs. In the
end, our findings deepened the understanding of molecular mechanisms of Ag NPs-
induced inflammation and provide assistance in the rational design of their
biomedical applications.
AN - WOS:000515380000014
AU - Li, H. Y.
AU - Wen, T.
AU - Wang, T.
AU - Ji, Y. L.
AU - Shen, Y. Y.
AU - Chen, J. Q.
AU - Xu, H. Y.
AU - Wu, X. C.
C7 - 384
DA - JAN 2
DO - 10.3390/ijms21020384
IS - 2
PY - 2020
SN - 1422-0067
ST - In Vivo Metabolic Response upon Exposure to Gold Nanorod Core/Silver Shell
Nanostructures: Modulation of Inflammation and Upregulation of Dopamine
TI - In Vivo Metabolic Response upon Exposure to Gold Nanorod Core/Silver Shell
Nanostructures: Modulation of Inflammation and Upregulation of Dopamine
VL - 21
ID - 6256
ER -
TY - JOUR
AB - With the increasing applications of silver nanoparticles (Ag NPs), the
concerns of widespread human exposure as well as subsequent health risks have been
continuously growing. The acute and chronic toxicities of Ag NPs in cellular tests
and animal tests have been widely investigated. Accumulating evidence shows that Ag
NPs can induce inflammation, yet the overall mechanism is incomplete. Herein, using
gold nanorod core/silver shell nanostructures (Au@Ag NRs) as a model system, we
studied the influence on mice liver and lungs from the viewpoint of metabolism. In
agreement with previous studies, Au@Ag NRs’ intravenous exposure caused
inflammatory reaction, accompanying with metabolic alterations, including energy
metabolism, membrane/choline metabolism, redox metabolism, and purine metabolism,
the disturbances of which contribute to inflammation. At the same time, dopamine
metabolism in liver was also changed. This is the first time to observe the
production of dopamine in non-neural tissue after treatment with Ag NPs. As the
upregulation of dopamine resists inflammation, it indicates the activation of
antioxidant defense systems against oxidative stress induced by Au@Ag NRs. In the
end, our findings deepened the understanding of molecular mechanisms of Ag NPs-
induced inflammation and provide assistance in the rational design of their
biomedical applications. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Li, H.
AU - Wen, T.
AU - Wang, T.
AU - Ji, Y.
AU - Shen, Y.
AU - Chen, J.
AU - Xu, H.
AU - Wu, X.
C7 - 384
DB - Scopus
DO - 10.3390/ijms21020384
IS - 2
KW - Dopamine
Gold nanorod core/silver shell nanostructures
Inflammation
Metabolism
Animals
Gold
Humans
Liver
Lung
Metal Nanoparticles
Mice
Nanostructures
Nanotubes
Oxidative Stress
Silver
dopamine
gold nanorod
silver nanoparticle
silver shell nanostructure
unclassified drug
gold
metal nanoparticle
nanomaterial
nanotube
silver
animal cell
animal experiment
animal tissue
Article
cell viability
choline metabolism
controlled study
dopamine metabolism
energy metabolism
female
in vitro study
in vivo study
inflammation
long term exposure
metabolism
metabolomics
molecular stability
mouse
nanoanalysis
near infrared spectroscopy
nonhuman
purine metabolism
RAW 264.7 cell line
redox metabolism
upregulation
animal
chemistry
drug effect
human
liver
lung
oxidative stress
pathology
M3 - Article
PY - 2020
ST - In vivo metabolic response upon exposure to gold nanorod core/silver shell
nanostructures: Modulation of inflammation and upregulation of dopamine
TI - In vivo metabolic response upon exposure to gold nanorod core/silver shell
nanostructures: Modulation of inflammation and upregulation of dopamine
85077898547&doi=10.3390%2fijms21020384&partnerID=40&md5=817e31fb2422c69b936b337ff36
44685
VL - 21
ID - 5265
ER -
TY - JOUR
AB - Background: Emodin is the main active component of rhubarb, which has
demonstrated many beneficial effects against inflammation. Nanosilver is an
effective antimicrobial agent. The present study was designed to observe the
effects of Emodin combined with silver nanoparticles (E/S) on sepsis protection and
related mechanism. Methods: E/S was prepared by loading different concentrations of
Emodin on nanosilver and cytotoxicity of E/S were determined by suphorhodamine B
assays. Anti-microbial activities of E/S were assayed by direct interaction with
various common pathogens and anti-adhesive activites of E/S on leukocytes with
endothelial cells were assayed by biochemical analysis. Next, inflammatory cell
enumeration, inflammatory mediators in bronchoalveolar lavage fluid (BALF) and
endothelial cell function were analyzed on a clinically relevant model of sepsis
induced by cecal ligation and puncture (CLP) after E/S administration. The effects
of E/S on NF-κB and p38 were also examined by western blot. Results: E/S exhibited
little cytotoxicity action on endothelial cells and significant inhibitory
activities against all tested common microorganisms and adherence between leukocyte
and endothelial cells. E/S induced anti-sepsis protection mainly mediated by
inhibition of inflammatory cells infiltration, down-regulation of TNF-alpha, IL-8
and lactic dehydrogenase (LDH), and inhibition of NF-κB and p38 pathways in mice 24
h post-CLP. Conclusion: Our data suggest that E/S has strong anti-sepsis effects,
which was related with anti-inflammatory protection and thereby promote survival
following sepsis challenge. © 2017 Li, Yang, Zhou, Du, Zhu and Sun.
AU - Li, H.
AU - Yang, T.
AU - Zhou, H.
AU - Du, J.
AU - Zhu, B.
AU - Sun, Z.
C7 - 536
DB - Scopus
DO - 10.3389/fphar.2016.00536
IS - JAN
KW - Emodin
Endothelial cells
Nanosilver
NF-κB
P38 MAPK
Sepsis
emodin
interleukin 6
interleukin 8
messenger RNA
silver nanoparticle
animal experiment
animal model
animal tissue
Article
cecal ligation and puncture-induced sepsis
cell adhesion
cell counting
cell infiltration
controlled study
cytotoxicity
down regulation
drug mechanism
enzyme activation
enzyme inhibition
fetus
gene expression
human
human cell
in vitro study
in vivo study
leukocyte
male
mouse
newborn
nonhuman
pneumonia
sepsis
Western blotting
M3 - Article
PY - 2017
ST - Emodin combined with nanosilver inhibited sepsis by anti-inflammatory
protection
TI - Emodin combined with nanosilver inhibited sepsis by anti-inflammatory
protection
85012054601&doi=10.3389%2ffphar.2016.00536&partnerID=40&md5=5af7f1635a161dd5e3c84a1
b2b7b168f
VL - 7
ID - 5479
ER -
TY - JOUR
AB - Background: Emodin is the main active component of rhubarb, which has
demonstrated many beneficial effects against inflammation. Nanosilver is an
effective antimicrobial agent. The present study was designed to observe the
effects of Emodin combined with silver nanoparticles (E/S) on sepsis protection and
related mechanism. Methods: E/S was prepared by loading different concentrations of
Emodin on nanosilver and cytotoxicity of E/S were determined by suphorhodamine B
assays. Anti-microbial activities of E/S were assayed by direct interaction with
various common pathogens and anti-adhesive activites of E/S on leukocytes with
endothelial cells were assayed by biochemical analysis. Next, inflammatory cell
enumeration, inflammatory mediators in bronchoalveolar lavage fluid (BALF) and
endothelial cell function were analyzed on a clinically relevant model of sepsis
induced by cecal ligation and puncture (CLP) after E/S administration. The effects
of E/S on NF-?B and p38 were also examined by western blot. Results: E/S exhibited
little cytotoxicity action on endothelial cells and significant inhibitory
activities against all tested common microorganisms and adherence between leukocyte
and endothelial cells. E/S induced anti-sepsis protection mainly mediated by
inhibition of inflammatory cells infiltration, down-regulation of TNF-alpha, IL-8
and lactic dehydrogenase (LDH), and inhibition of NF-?B and p38 pathways in mice 24
h post-CLP. Conclusion: Our data suggest that E/S has strong anti-sepsis effects,
which was related with anti-inflammatory protection and thereby promote survival
following sepsis challenge.
AN - WOS:000391407800001
AU - Li, H.
AU - Yang, T.
AU - Zhou, H.
AU - Du, J.
AU - Zhu, B.
AU - Sun, Z. M.
C7 - 536
DA - JAN 10
DO - 10.3389/fphar.2016.00536
PY - 2017
SN - 1663-9812
ST - Emodin Combined with Nanosilver Inhibited Sepsis by Anti-inflammatory
Protection
T2 - FRONTIERS IN PHARMACOLOGY
TI - Emodin Combined with Nanosilver Inhibited Sepsis by Anti-inflammatory
Protection
VL - 7
ID - 6033
ER -
TY - JOUR
AB - The extensive use of antibiotics causes drug resistance, which slows the
healing process in wound infections and this calls for an urgent response to the
development of novel therapeutic approaches. However, it is challenging to develop
materials with intrinsic and effective antibacterial properties for enhanced wound
healing. Here, a highly efficient, bacteria-responsive and controlled-release
silver ion coating was constructed by the assembly of quaternized chitosan (QCS),
tannic acid (TA), and silver ions using the layer-by-layer method. The coating
platform exhibited a distinct "self-defense" behavior triggered by acidification
and good antibacterial activity against both gram-negative and gram-positive
bacteria, which was further enhanced by the positively charged groups of QCS and
the release of silver ions. Moreover, the coatings exhibited excellent
biocompatibility, highlighted by promoting both fibroblast and endothelial cell
proliferation, supporting fibroblast migration, suppressing macrophage activation,
and modulating inflammatory cytokine release. Notably, animal experimental results
further revealed that the coatings terminated bleeding, inhibited bacterial growth
and accelerated healing at the wound site. Collectively, the coatings showed
outstanding hemostatic performance and antimicrobial activity as well as improved
wound healing process, which might be as a promising wound treatment platform.
AN - WOS:000760480900002
AU - Li, L. H.
AU - Liu, L. Y.
AU - Li, L.
AU - Guo, F.
AU - Ma, L.
AU - Fu, P.
AU - Wang, Y. B.
C6 - FEB 2022
C7 - 109665
DA - APR 1
DO - 10.1016/j.compositesb.2022.109665
PY - 2022
SN - 1359-8368
1879-1069
ST - Chitosan coated bacteria responsive metal-polyphenol coating as efficient
platform for wound healing
T2 - COMPOSITES PART B-ENGINEERING
TI - Chitosan coated bacteria responsive metal-polyphenol coating as efficient
VL - 234
ID - 6692
ER -
TY - JOUR
AB - Owing to the excellent antibacterial and antiviral activity, silver
nanoparticles have a widespread use in the food and pharmaceutical industries. With
the increase in the production and use of the related products, the potential
hazard of silver nanoparticles has aroused public attention. The main purpose of
this study is to explore the toxicity of silver nanoparticles and induction of lung
inflammation in vitro and in vivo. Here, we validated that small amounts of silver
ions dissolved from silver nanoparticles caused the depolarization of plasma
membrane, resulting in an overload of intracellular sodium and calcium, and
eventually led to the cell necrosis. The blockers of calcium or sodium channels
inversed the toxicity of silver ions. Then, we instilled silver nanoparticles or
silver nitrate (50 mu g per mouse) into the lungs of mice, and this induced
pulmonary injury and mitochondrial content release, led to the recruitment of
neutrophils to the lung tissue via p38 MAPK pathway. Altogether, these data show
that released silver ions from nanoparticles induced cell necrosis through Na+ and
Ca2+ influx and triggered pulmonary inflammation through elevating mitochondrial-
related contents released from these necrotic cells.
AN - WOS:000530254300001
AU - Li, L.
AU - Bi, Z. F.
AU - Hu, Y. H.
AU - Sun, L.
AU - Song, Y. L.
AU - Chen, S. Y.
AU - Mo, F.
AU - Yang, J. Y.
AU - Wei, Y. Q.
AU - Wei, X. W.
C6 - MAY 2020
DA - APR
DO - 10.1007/s10565-020-09526-4
IS - 2
PY - 2021
SN - 0742-2091
1573-6822
SP - 177-191
ST - Silver nanoparticles and silver ions cause inflammatory response through
induction of cell necrosis and the release of mitochondria in vivo and in vitro
TI - Silver nanoparticles and silver ions cause inflammatory response through
VL - 37
ID - 5872
ER -
TY - JOUR
AB - Owing to the excellent antibacterial and antiviral activity, silver
nanoparticles have a widespread use in the food and pharmaceutical industries. With
the increase in the production and use of the related products, the potential
hazard of silver nanoparticles has aroused public attention. The main purpose of
this study is to explore the toxicity of silver nanoparticles and induction of lung
inflammation in vitro and in vivo. Here, we validated that small amounts of silver
ions dissolved from silver nanoparticles caused the depolarization of plasma
membrane, resulting in an overload of intracellular sodium and calcium, and
eventually led to the cell necrosis. The blockers of calcium or sodium channels
inversed the toxicity of silver ions. Then, we instilled silver nanoparticles or
silver nitrate (50 μg per mouse) into the lungs of mice, and this induced pulmonary
injury and mitochondrial content release, led to the recruitment of neutrophils to
the lung tissue via p38 MAPK pathway. Altogether, these data show that released
silver ions from nanoparticles induced cell necrosis through Na+ and Ca2+ influx
and triggered pulmonary inflammation through elevating mitochondrial-related
contents released from these necrotic cells. © 2020, Springer Nature B.V.
AU - Li, L.
AU - Bi, Z.
AU - Hu, Y.
AU - Sun, L.
AU - Song, Y.
AU - Chen, S.
AU - Mo, F.
AU - Yang, J.
AU - Wei, Y.
AU - Wei, X.
DB - Scopus
DO - 10.1007/s10565-020-09526-4
IS - 2
KW - Necrosis
Pulmonary inflammation
Silver ions
A549 Cells
Animals
Calcium
DNA, Mitochondrial
GTPase-Activating Proteins
Humans
Ions
Metal Nanoparticles
Mice, Inbred C57BL
Mitochondria
N-Formylmethionine Leucyl-Phenylalanine
Necroptosis
Pneumonia
Protein Kinases
Silver
Sodium
calcium
cation
silver
silver nanoparticle
silver nitrate
sodium
sodium channel blocking agent
formylmethionylleucylphenylalanine
guanosine triphosphatase activating protein
ion
metal nanoparticle
mitochondrial DNA
MLKL protein, mouse
protein kinase
Ralbp1 protein, mouse
acute toxicity
animal cell
animal experiment
animal model
animal tissue
Article
calcium cell level
calcium transport
cell death
cell membrane depolarization
controlled study
female
human
human cell
in vitro study
in vivo study
ion transport
lung injury
lung parenchyma
lung toxicity
MAPK signaling
mitochondrion
mouse
neutrophil
nonhuman
pneumonia
priority journal
sodium cell level
sodium transport
A-549 cell line
animal
C57BL mouse
drug effect
metabolism
necroptosis
necrosis
pathology
ultrastructure
M3 - Article
PY - 2021
SP - 177-191
ST - Silver nanoparticles and silver ions cause inflammatory response through
T2 - Cell Biology and Toxicology
TI - Silver nanoparticles and silver ions cause inflammatory response through
85085145945&doi=10.1007%2fs10565-020-09526-
4&partnerID=40&md5=d28df70fae05100c5f30459b0c033d0d
VL - 37
ID - 5223
ER -
TY - JOUR
AB - The extensive use of antibiotics causes drug resistance, which slows the
healing process in wound infections and this calls for an urgent response to the
development of novel therapeutic approaches. However, it is challenging to develop
materials with intrinsic and effective antibacterial properties for enhanced wound
healing. Here, a highly efficient, bacteria-responsive and controlled-release
silver ion coating was constructed by the assembly of quaternized chitosan (QCS),
tannic acid (TA), and silver ions using the layer-by-layer method. The coating
platform exhibited a distinct “self-defense” behavior triggered by acidification
and good antibacterial activity against both gram-negative and gram-positive
bacteria, which was further enhanced by the positively charged groups of QCS and
the release of silver ions. Moreover, the coatings exhibited excellent
biocompatibility, highlighted by promoting both fibroblast and endothelial cell
proliferation, supporting fibroblast migration, suppressing macrophage activation,
and modulating inflammatory cytokine release. Notably, animal experimental results
further revealed that the coatings terminated bleeding, inhibited bacterial growth
and accelerated healing at the wound site. Collectively, the coatings showed
outstanding hemostatic performance and antimicrobial activity as well as improved
wound healing process, which might be as a promising wound treatment platform. ©
2022 Elsevier Ltd
AU - Li, L.
AU - Liu, L.
AU - Guo, F.
AU - Ma, L.
AU - Fu, P.
AU - Wang, Y.
C7 - 109665
DB - Scopus
DO - 10.1016/j.compositesb.2022.109665
KW - Antibacterial
Bacteria responsive
Layer-by-Layer method
Quaternized chitosan
Wound healing
Bacteria
Biocompatibility
Cell culture
Cell proliferation
Chitosan
Endothelial cells
Fibroblasts
Metal ions
Silver
Antibacterials
Drug-resistance
Healing process
Layer-by-layer methods
Polyphenols
Quaternized chitosans
Silver ions
Wound infections
Coatings
M3 - Article
PY - 2022
ST - Chitosan coated bacteria responsive metal-polyphenol coating as efficient
T2 - Composites Part B: Engineering
TI - Chitosan coated bacteria responsive metal-polyphenol coating as efficient
85124236913&doi=10.1016%2fj.compositesb.2022.109665&partnerID=40&md5=92c06a47666b02
eefaab5d42ec34fb90
VL - 234
ID - 5133
ER -
TY - JOUR
AB - Effective coverage and protection is a priority in wound treatment. Collagen
and chitosan have been widely used for wound dressings due to their excellent
biological activity and biocompatibility. Silver nanoparticles (AgNPs) have a
powerful antibacterial effect. In this study, a macromolecular and small-molecular
collagen mixed solution, a macromolecular and small-molecular chitosan mixed
solution were prepared, and a silver nanoparticle-loaded collagen-chitosan dressing
(AgNP-CCD) has been proposed. First, the effects of a collagen-chitosan mixed
solution on the proliferation of human umbilical vein endothelial cells and the
secretion of cytokines were evaluated. Then, the characteristics and antibacterial
effects of the AgNP-CCD were tested, and the effects on wound healing and the
influence of wound cytokine expression were investigated via a deep second-degree
burn wound model. The results showed that at the proper proportion and
concentration, the collagen-chitosan mixed solution effectively promoted cell
proliferation and regulated the levels of growth factors (vascular endothelial
growth factor [VEGF], epidermal growth factor [EGF], platelet-derived growth factor
[PDGF], transforming growth factor [TGF-beta 1], basic fibroblastic growth factor
[bFGF]) and inflammatory factors (TNF-alpha, IL-1 beta, IL-6, IL-8). Moreover, AgNP
solutions at lower concentrations exerted limited inhibitory effects on cell
proliferation and had no effect on cytokine secretion. The AgNP-CCD demonstrated
satisfactory morphological and physical properties as well as efficient
antibacterial activities. An in vivo evaluation indicated that AgNP-CCD could
accelerate the healing process of deep second-degree burn wounds and played an
important role in the regulation of growth and inflammatory factors, including
VEGF, EGFL-7, TGF-beta 1, bFGF, TNF-alpha and IL-1 beta. This AgNP-CCD exerted
excellent biological effects on wound healing promotion and cytokine expression
regulation.
AN - WOS:000593175800004
AU - Li, R. F.
AU - Xu, Z. R.
AU - Jiang, Q.
AU - Zheng, Y. Q.
AU - Chen, Z. H.
AU - Chen, X. D.
DA - AUG
DO - 10.1093/rb/rbaa008
IS - 4
PY - 2020
SN - 2056-3418
2056-3426
SP - 371-380
ST - Characterization and biological evaluation of a novel silver nanoparticle-
loaded collagen-chitosan dressing
T2 - REGENERATIVE BIOMATERIALS
TI - Characterization and biological evaluation of a novel silver nanoparticle-
VL - 7
ID - 6158
ER -
TY - JOUR
AB - Effective coverage and protection is a priority in wound treatment. Collagen
and chitosan have been widely used for wound dressings due to their excellent
biological activity and biocompatibility. Silver nanoparticles (AgNPs) have a
powerful antibacterial effect. In this study, a macromolecular and small-molecular
collagen mixed solution, a macromolecular and small-molecular chitosan mixed
solution were prepared, and a silver nanoparticle-loaded collagen-chitosan dressing
(AgNP-CCD) has been proposed. First, the effects of a collagen-chitosan mixed
solution on the proliferation of human umbilical vein endothelial cells and the
secretion of cytokines were evaluated. Then, the characteristics and antibacterial
effects of the AgNP-CCD were tested, and the effects on wound healing and the
influence of wound cytokine expression were investigated via a deep second-degree
burn wound model. The results showed that at the proper proportion and
concentration, the collagen-chitosan mixed solution effectively promoted cell
proliferation and regulated the levels of growth factors (vascular endothelial
growth factor [VEGF], epidermal growth factor [EGF], platelet-derived growth factor
[PDGF], transforming growth factor [TGF-b1], basic fibroblastic growth factor
[bFGF]) and inflammatory factors (TNF-a, IL-1b, IL-6, IL-8). Moreover, AgNP
solutions at lower concentrations exerted limited inhibitory effects on cell
proliferation and had no effect on cytokine secretion. The AgNP-CCD demonstrated
satisfactory morphological and physical properties as well as efficient
antibacterial activities. An in vivo evaluation indicated that AgNP-CCD could
accelerate the healing process of deep second-degree burn wounds and played an
important role in the regulation of growth and inflammatory factors, including
VEGF, EGFL-7, TGF-b1, bFGF, TNF-a and IL-1b. This AgNP-CCD exerted excellent
biological effects on wound healing promotion and cytokine expression regulation. ©
The Author(s) 2020.
AU - Li, R.
AU - Xu, Z.
AU - Jiang, Q.
AU - Zheng, Y.
AU - Chen, Z.
AU - Chen, X.
DB - Scopus
DO - 10.1093/RB/RBAA008
IS - 4
KW - Chitosan
Collagen
Cytokines
Wound dressing
Wound healing
Biocompatibility
Cell engineering
Cell proliferation
Endothelial cells
Macromolecules
Metal nanoparticles
Tissue regeneration
chitosan
collagen
interleukin 1beta
interleukin 6
interleukin 8
silver nanoparticle
transforming growth factor
vasculotropin
Epidermal growth factors
Human umbilical vein endothelial cells
Platelet-derived growth factors
Transforming growth factors
Vascular endothelial growth factor
animal experiment
animal model
Article
burn
cell proliferation
cellular secretion
controlled study
cytokine release
female
human
human cell
in vivo study
nonhuman
priority journal
protein expression
protein function
rat
wound healing
Bioactivity
M3 - Article
PY - 2021
SP - 371-380
ST - Characterization and biological evaluation of a novel silver nanoparticle-
T2 - Regenerative Biomaterials
TI - Characterization and biological evaluation of a novel silver nanoparticle-
85096958893&doi=10.1093%2fRB
%2fRBAA008&partnerID=40&md5=e363d0fd79908b2c87ef5c4af26de69e
VL - 7
ID - 5324
ER -
TY - JOUR
AB - Background. Silver nanoparticles (AgNPs) have been widely used in many
commercial products due to their excellent antibacterial ability. The AgNPs are
released into the environment, gradually accumulate in the ocean, and may affect
animals at high trophic levels, such as cetaceans and humans, via the food chain.
Hence, the negative health impacts caused by AgNPs in cetaceans are of concern.
Cytokines play a major role in the modulation of immune system and can be
classified into two types: Th1 and Th2. Th1/Th2 balance can be evaluated by the
ratios of their polarizing cytokines (i.e., interferon [IFN]-γ/Interleukin [IL]-4),
and animals with imbalanced Th1/Th2 response may become more susceptible to certain
kinds of infection. Therefore, the present study evaluated the in vitro cytokine
responses of cetacean peripheral blood mononuclear cells (cPBMCs) to 20 nm citrate-
AgNPs (C-AgNP20) by quantitative reverse transcriptase polymerase chain reaction
(qRT-PCR). Methods. Blood samples were collected from six captive common bottlenose
dolphins (Tursiops truncatus). The cPBMCs were isolated and utilized for evaluating
the in vitro cytokine responses. The cytokines evaluated included IL-2, IL-4, IL-
10, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α. The geometric
means of two housekeeping genes (HKGs), glyceraldehyde 3-phosphate dehydrogenase
(GAPDH) and β2-microglobulin (B2M), of each sample were determined and used to
Normalize the mRNA expression levels of target genes. Results. The ratio of late
apoptotic/necrotic cells of cPBMCs significantly increased with or without
concanavalin A (ConA) stimulation after 24 h of 10 µg/ml C-AgNP20 treatment. At 4 h
of culture, the mRNA expression level of IL-10 was significantly decreased with 1
µg/ml C-AgNP20 treatment. At 24 h of culture with 1 µg/ml C-AgNP20, the mRNA
expression levels of all cytokines were significantly decreased, with the
exceptions of IL-4 and IL-10. The IFN-γ/IL-4 ratio was significantly decreased at
24 h of culture with 1 µg/ml C-AgNP20 treatment, and the IL-12/IL-4 ratio was
significantly decreased at 4 or 24 h of culture with 0.1 or 1 µg/ml C-AgNP20
treatment, respectively. Furthermore, the mRNA expression level of TNF-α was
significantly decreased by 1 µg/ml C-AgNP20 after 24 h of culture. Discussion. The
present study demonstrated that the sublethal dose of C-AgNP20 (≤1 µg/ml) had an
inhibitory effect on the cytokine mRNA expression levels of cPBMCs with the
evidence of Th2 cytokine bias and significantly decreased the mRNA expression level
of TNF-α. Th2 cytokine bias is associated with enhanced immunity against parasites
but decreased immunity to intracellular microorganisms. TNF-α is a contributing
factor for the inflammatory response against the infection of intracellular
pathogens. In summary, our data indicate that C-AgNP20 suppresses the cellular
immune response and thereby increases the susceptibility of cetaceans to infection
by intracellular microorganisms. © 2018 Li et al.
AU - Li, W. T.
AU - Wang, L. Y.
AU - Chang, H. W.
AU - Yang, W. C.
AU - Lo, C.
AU - Pang, V. F.
AU - Chen, M. H.
AU - Jeng, C. R.
C7 - e5432
DB - Scopus
DO - 10.7717/peerj.5432
IS - 9
KW - Cetacean
Cytokine
Immunotoxicity
QRT-PCR
Silver nanoparticles (AgNPs)
Th2 bias
beta 2 microglobulin
gamma interferon
interleukin 10
interleukin 12
interleukin 2
interleukin 4
messenger RNA
silver nanoparticle
animal cell
animal experiment
animal model
animal tissue
apoptosis assay
Article
blood biochemistry
blood cell count
blood sampling
bottlenose dolphin
cell migration assay
controlled study
cytokine response
cytotoxicity
flow cytometry
gene expression
housekeeping gene
immune response
macrophage
nonhuman
oxidative stress
receptor down regulation
T lymphocyte
Th1 Th2 balance
Th2 cell
zeta potential
M3 - Article
PY - 2018
ST - Th2 cytokine bias induced by silver nanoparticles in peripheral blood
mononuclear cells of common bottlenose dolphins (Tursiops truncatus)
T2 - PeerJ
TI - Th2 cytokine bias induced by silver nanoparticles in peripheral blood
mononuclear cells of common bottlenose dolphins (Tursiops truncatus)
85053391175&doi=10.7717%2fpeerj.5432&partnerID=40&md5=2f109415887ea750ead34c38efcff
2f4
VL - 2018
ID - 5536
ER -
TY - JOUR
AB - Bacterial infection is one of the most critical obstacles in wound healing,
and severe bacterial infections can lead to inflammatory conditions and delay the
healing process. Herein, a novel hydrogel based on polyvinyl alcohol (PVA), agar,
and silk-AgNPs was prepared using a straightforward one-pot physical cross-linking
method. The in situ synthesis of AgNPs in hydrogels exploited the reducibility of
tyrosine (Tyr tyrosine) in silk fibroin, which endowed the hydrogels with
outstanding antibacterial qualities. In addition, the strong hydrogen bond cross-
linked networks of agar and the crystallites formed by PVA as the physical cross-
linked double network of the hydrogel gave it excellent mechanical stability. The
PVA/agar/SF-AgNPs (PASA) hydrogels exhibited excellent water absorption, porosity,
and significant antibacterial effects against Escherichia coli (E. coli) and
Staphylococcus aureus (S. aureus). Furthermore, in vivo experimental results
confirmed that the PASA hydrogel significantly promoted wound repair and skin
tissue reconstruction by reducing inflammation and promoting collagen deposition.
Immunofluorescence staining showed that the PASA hydrogel enhanced CD31 expression
to promote angiogenesis while decreasing CD68 expression to reduce inflammation.
Overall, the novel PASA hydrogel showed great potential for bacterial infection
wound management. © 2023
AU - Li, W.
AU - Wu, Z.
AU - Zhao, J.
AU - Jiang, M.
AU - Yuan, L.
AU - Guo, Y.
AU - Li, S.
AU - Hu, L.
AU - Xie, X.
AU - Zhang, Y.
AU - Tao, G.
AU - Cai, R.
C7 - 125652
DB - Scopus
DO - 10.1016/j.ijbiomac.2023.125652
KW - Antibacterial activity
Dual physically cross-linked hydrogels
Infected wound repair
Mechanical stability
Wound dressing
Algae
Amino acids
Biomechanics
Escherichia coli
Hydrogen bonds
Pathology
Polysaccharides
Polyvinyl alcohols
Repair
agar
CD68 antigen
collagen
hydrogel
polyvinyl alcohol
silk
silk fibroin
silver nanoparticle
tyrosine
Cross-linked hydrogels
Dual physically cross-linked hydrogel
Infected wounds
Inflammatory conditions
Wound dressings
Wound healing
Wound repair
angiogenesis
animal cell
animal experiment
animal model
animal tissue
Article
biocompatibility
controlled study
cross linking
hemolysis
human
human cell
HUVEC cell line
hydrogen bond
hydrophilicity
in vitro study
in vivo study
inflammation
Leporidae
male
mouse
nanofabrication
nonhuman
one pot synthesis
porosity
protein expression
rat
skin fibroblast
skin injury
tensile strength
therapy effect
water absorption
wound healing
wound infection
Young modulus
Hydrogels
M3 - Article
PY - 2023
ST - Fabrication of dual physically cross-linked polyvinyl alcohol/agar hydrogels
with mechanical stability and antibacterial activity for wound healing
TI - Fabrication of dual physically cross-linked polyvinyl alcohol/agar hydrogels
with mechanical stability and antibacterial activity for wound healing
85165116916&doi=10.1016%2fj.ijbiomac.2023.125652&partnerID=40&md5=f029be98ea901d7c7
77f9d7ed6696039
VL - 247
ID - 4994
ER -
TY - JOUR
AB - The functional activities of gold nanoparticles (AuNPs) on biological systems
depend on their physical-chemical properties and their surface functionalizations.
Within a biological environment and depending on their surface characteristics, NPs
can adsorb biomolecules (mostly proteins) present in the microenvironment, thereby
forming a dynamic biomolecular corona on the surface. The presence of this
biocorona changes the physicalchemical and functional properties of the NPs and how
it interacts with cells. Here, we show that primary human epidermal keratinocytes
(HER) exposed in culture to branched polyethyleneimine (BPEI)-AuNPs, but not to
lipoic acid (LA)-AuNPs, show potent particle uptake, decreased cell viability and
enhanced production of inflammatory factors, while the presence of a human plasma-
derived biocorona decreased NPs uptake and rescued cells from BPEI-AuNP-induced
cell death. The mechanistic study revealed that the intracellular oxidative level
greatly increased after the BPEI-AuNPs treatment, and the transcriptomic analysis
showed that the dominant modulated pathways were related to oxidative stress and an
antioxidant response. The stress level measured by flow cytometry also showed a
significant decrease in the presence of a biocorona. Further anaylsis discovered
that nuclear factor erythroid-2 related factor (Nrf2), a major regulator of anti-
oxidant and anti-inflammatory genes, as the key factor related to the AuNPs induced
oxidative stress and inflammation. This study provides futher understanding into
the mechanisms on how NPs-induced cellular stress and reveals the protective
effects of a biocorona on inflammatory responses in HER at the molecular level,
which provides important insights into the biological responses of AuNPs and their
biocorona.
AN - WOS:000856037000001
AU - Li, X. J.
AU - Li, D. J.
AU - Zhang, G. F.
AU - Zeng, Y. Q.
AU - Monteiro-Riviere, N. A.
AU - Chang, Y. Z.
AU - Li, Y.
C6 - SEP 2022
DA - OCT 1
DO - 10.1016/j.toxlet.2022.08.009
PY - 2022
SN - 0378-4274
1879-3169
SP - 34-42
ST - Biocorona modulates the inflammatory response induced by gold nanoparticles
in human epidermal keratinocytes
TI - Biocorona modulates the inflammatory response induced by gold nanoparticles
in human epidermal keratinocytes
VL - 369
ID - 6436
ER -
TY - JOUR
AB - Recently gold nanomaterials have been widely applied in the biomedical field,
but their biosafety is still controversial. We immobilized small gold nanoparticles
(AuNPs) on a large silica substrate to form silica-gold core-shell materials
(SiO2@AuNPs) via classical seed-mediated growth. In vitro, 500 nm-SiO2@AuNPs could
promote the proliferation of mouse embryonic fibroblast cells (NIH/3T3). The
results of transmission electron microscope (TEM) showed that the vast majority of
particles did not enter cells and that the morphology of microtubules experienced
no change as observed in the confocal microscope images. The mechanism may be that
the large silica substrate kept AuNPs outside the cells and the nanosize concavo-
convex gold shell facilitated to cell adhesion, resulting in the proliferation. In
vivo, a cutaneous full-thickness excisional wound rat model was applied to assess
the healing efficiency of 500 nm-SiO2@AuNPs. The results indicated that SiO2@AuNPs
could promote wound healing, which was potentially related to the anti-inflammatory
and antioxidation of AuNPs. The pathological finding showed that the healing levels
of SiO2@AuNPs were significantly better than those of the control groups. Our study
may provide insight into the application of silica-gold core-shell materials in the
treatment of cutaneous wounds. (C) 2015 Elsevier Inc. All rights reserved.
AN - WOS:000350006700037
AU - Li, X. Q.
AU - Wang, H. F.
AU - Rong, H. L.
AU - Li, W. H.
AU - Luo, Y.
AU - Tian, K.
AU - Quan, D. Q.
AU - Wang, Y. G.
AU - Jiang, L.
DA - MAY 1
DO - 10.1016/j.jcis.2014.12.084
PY - 2015
SN - 0021-9797
1095-7103
SP - 312-319
ST - Effect of composite SiO2@AuNPs on wound healing: In vitro and vivo studies
T2 - JOURNAL OF COLLOID AND INTERFACE SCIENCE
TI - Effect of composite SiO2@AuNPs on wound healing: In vitro and vivo studies
VL - 445
ID - 6588
ER -
TY - JOUR
AB - The sequence of p38MAPK in silver carp (Hm-p38a) was cloned and sequenced.
Additionally, the acute toxicity of crude microcystins (MCs) on silver carp and
induction expression of Hm-p38a by MCs exposure were also determined in this study.
The results reveal that the length of Hm-p38a is 2418 bp and it contains a 1086 bp
open reading frame. Hm-p38a could encode 361 amino acids. Sequence analysis
indicates that Hm-p38a contains the conserved structures of Thr-Gly-Tyr motif and
substrate binding site Ala-Thr-Arg-Trp, and it is highly conserved in fish.
Phylogenetic analysis reveals that Hm-p38a is more closely related to fish than
mammals and it belongs to p38 alpha subfamily. Moreover, Hm-p38a in silver carp is
constitutively expressed in all examined tissues. In addition, our results indicate
that MCs exposure significantly promotes the transcription of Hm-p38a in fish liver
or kidney, suggesting that mitogen-activated protein kinase should also be the
signal pathway of MCs hepatotoxicity in fish. (C) 2016 Wiley Periodicals, Inc.
AN - WOS:000378507100002
AU - Li, X. Y.
AU - Ma, J. G.
AU - Li, Y. Y.
DA - MAY
DO - 10.1002/jbt.21781
IS - 5
PY - 2016
SN - 1095-6670
1099-0461
SP - 224-231
ST - Molecular Cloning and Expression Determination of p38 MAPK from the Liver and
Kidney of Silver Carp
T2 - JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
TI - Molecular Cloning and Expression Determination of p38 MAPK from the Liver and
Kidney of Silver Carp
VL - 30
ID - 6407
ER -
TY - JOUR
AB - Microcystins produced by some cyanobacteria can cause damages to the liver
and kidneys of aquatic animals. In the natural water with cyanobacterial blooms,
silver carp may suffer from the most serious affect of the bloom due to their
filtering these cyanobacteria and ingesting them as food. In the present study,
silver carp was exposed to microcystin-LR by using the method of intraperitoneal
injection first to determine the acute toxicity of microcystin-LR on silver carp
and then to determine the activity of inflammatory protein and content of
inflammatory factors from the serum of silver carp following a subacute exposure of
microcystin-LR at doses of 104.9 μg kg –1 (1/5 of LD 50 ) or 262.1 μg kg –1 (1/2 of
LD 50 ). The results showed that MC-LR exposure increased fish liver index and
promoted the activities of fish serum alanine aminotransferase (ALT) and aspartate
aminotransferase (AST), indicating the hepatotoxicity of MC-LR on the fish.
Moreover, MC-LR exposure also increased the number of leukocytes, complement C3
level, lysozyme activity (at the first 9 h of exposure), and the contents of
cytokines TNF-α IL-1β and IFN-γ in fish serum. In addition, a significant increase
in IgM level was observed in the serum and head kidney of silver carp following MC-
LR exposure. This result suggests that semi-lethal doses of MC-LR exposure is not
only hepatotoxic but also immunotoxic to silver carp. © 2018 Elsevier Inc.
AU - Li, X.
AU - Li, J.
AU - Meng, F.
AU - Yao, L.
DB - Scopus
DO - 10.1016/j.ecoenv.2018.10.110
KW - Acute hepatotoxicity
Immunotoxicity
Microcystin-LR
Silver carp
Animals
Aspartate Aminotransferases
Carps
Cytokines
Head Kidney
Immunoglobulin M
Leukocyte Count
Liver
Microcystins
Animalia
Cyanobacteria
Hypophthalmichthys nobilis
gamma interferon
immunoglobulin M
interleukin 1beta
lysozyme
microcystin LR
autacoid
cytokine
microcystin
cyanobacterium
cyprinid
enzyme activity
immune response
pollution exposure
toxicity
adaptive immunity
animal cell
animal experiment
animal model
animal tissue
Article
carp
complement blood level
controlled study
head kidney
immunoglobulin blood level
immunotoxicity
innate immunity
LD50
leukocyte count
liver toxicity
nonhuman
protein content
silver carp
animal
blood
drug effect
immunology
liver
metabolism
M3 - Article
PY - 2019
SP - 28-32
ST - Hepatotoxicity and immunotoxicity of MC-LR on silver carp
TI - Hepatotoxicity and immunotoxicity of MC-LR on silver carp
85056165094&doi=10.1016%2fj.ecoenv.2018.10.110&partnerID=40&md5=04af276cc17e72c9895
459693b18476c
VL - 169
ID - 5417
ER -
TY - JOUR
AB - Infection in bone transplantation process is attracting considerable
attention. The current study synthesizes silver/strontium co-substituted
hydroxyapatite (Ag/Sr-HA) nanoparticles with combined osteogenic and antibacterial
activities. Different concentrations of silver-substituted hydroxyapatite (Ag-HA)
nanoparticles were synthesized by hydrothermal method, and then their
physicochemical properties were characterized by X-ray powder diffraction (XRD),
Fourier transform infrared spectroscopy (FTIR), transmission electron microscope
(TEM), and energy-dispersive X-ray spectroscopy (EDS). Then, Sr was added as
secondary element into Ag-HA to improve the biocompatibility of substrate. The
antibacterial experiments indicated that Ag-HA had excellent antibacterial activity
against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The
effects of prepared samples on cell proliferation and differentiation were
evaluated using MC3T3-E1 cells in vitro. The results showed that Sr substitution
enhanced cell proliferation and differentiation, upregulated expression of
osteogenic genes, and induced mineralization of cells. The substitution of Sr in
Ag/Sr-HA nanoparticles can effectively alleviate the negative effects of Ag and
enhance the biological activity of HA. Thus, the synthesized Ag/Sr-HA nanoparticles
will serve as a potential candidate for application of biomedical implants with
excellent osteogenic and antibacterial ability.
AN - WOS:000636146900002
AU - Li, Y. F.
AU - Wang, W. Y.
AU - Han, J.
AU - Li, Z. R.
AU - Wang, Q. X.
AU - Lin, X.
AU - Ge, K.
AU - Zhou, G. Q.
C6 - APR 2021
DA - FEB
DO - 10.1007/s12011-021-02697-z
IS - 2
PY - 2022
SN - 0163-4984
1559-0720
SP - 931-942
ST - Synthesis of Silver- and Strontium-Substituted Hydroxyapatite with Combined
Osteogenic and Antibacterial Activities
TI - Synthesis of Silver- and Strontium-Substituted Hydroxyapatite with Combined
Osteogenic and Antibacterial Activities
VL - 200
ID - 6374
ER -
TY - JOUR
AB - Recent study suggested that the presence of phytochemicals in food could
interact with nanoparticles (NPs) and consequently reduce the toxicity of NPs,
which has been attributed to the antioxidant properties of phytochemicals. In this
study, we investigated the interactions between ZnO NPs and two flavonoids
baicalein (Ba) or baicalin (Bn) as well as the influence of the interactions on the
toxicity of ZnO NPs to Caco-2 cells. The antioxidant properties of Ba and Bn were
confirmed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-azino-bis(3-
ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, with Ba being stronger.
However, the presence of Ba or Bn did not significantly affect cytotoxicity,
intracellular superoxide or release of inflammatory cytokines of Caco-2 cells after
ZnO NP exposure. When Ba was present, the cellular viability of Caco-2 cells after
exposure to ZnO NPs was slightly increased, associated with a modest decrease of
intracellular Zn ions, but these effects were not statistically different. Ba was
more effective than Bn at changing the hydrodynamic sizes, Zeta potential and UV-
Vis spectra of ZnO NPs, which indicated that Ba might increase the colloidal
stability of NPs. Taken together, the results of the present study indicated that
the anti-oxidative phytochemical Ba might only modestly protected Caco-2 cells from
the exposure to ZnO NPs associated with an insignificant reduction of the
accumulation of intracellular Zn ions. These results also indicated that when
assessing the combined effects of NPs and phytochemicals to cells lining
gastrointestinal tract, it might be necessary to evaluate the changes of colloidal
stability of NPs altered by phytochemicals.
AN - WOS:000423633100002
AU - Li, Y. N.
AU - Zhang, C.
AU - Liu, L. L.
AU - Gong, Y.
AU - Xie, Y. X.
AU - Cao, Y.
DO - 10.1080/15376516.2017.1376023
IS - 3
PY - 2018
SN - 1537-6516
1537-6524
SP - 167-176
ST - The effects of baicalein or baicalin on the colloidal stability of ZnO
nanoparticles (NPs) and toxicity of NPs to Caco-2 cells
T2 - TOXICOLOGY MECHANISMS AND METHODS
TI - The effects of baicalein or baicalin on the colloidal stability of ZnO
nanoparticles (NPs) and toxicity of NPs to Caco-2 cells
VL - 28
ID - 6346
ER -
TY - JOUR
AB - Implant-associated infections are a major factor contributing to graft
failure. Preventing infection by inhibiting bacterial adhesion to implants is
critical for successful orthopedic surgery. In this work, we fabricated a novel
implant material and evaluated its performance in terms of inhibiting bacterial
growth and adhesion. Micro/nano-structured titanium (MNT) was prepared by the
micro-arc oxidization. Silver nanoparticles (AgNPs) with an average diameter of 10
nm were synthesized via a chemical reduction method and immobilized onto MNT to
generate an AgNP-decorated MNT (AgMN). It showed high antibacterial efficacy
against both Escherichia coli and Staphylococcus aureus without cytotoxicity to
NIH/3T3 fibroblast-like cells. AgMN implanted in rats inhibited S. aureus growth
and adhesion and elicited a milder inflammatory response than MNT. Moreover,
implanted AgMN had no adverse effect on the morphology and structure of attached
host cells after 1 day. These results indicate that using AgMN as an implant
material can reduce the risk of infection without toxic effects to the host.
AN - WOS:000432853100005
AU - Li, Y. T.
AU - Tan, J.
AU - Liu, Z. Y.
AU - Wang, G. S.
AU - Zhao, C. J.
AU - Duan, K.
AU - Weng, J.
AU - Feng, B.
DA - APR
DO - 10.1166/jbn.2018.2482
IS - 4
PY - 2018
SN - 1550-7033
1550-7041
SP - 675-687
ST - Antibacterial Activity and Cyto-/Tissue-Compatibility of Micro-/Nano-
Structured Titanium Decorated with Silver Nanoparticles
TI - Antibacterial Activity and Cyto-/Tissue-Compatibility of Micro-/Nano-
Structured Titanium Decorated with Silver Nanoparticles
VL - 14
ID - 5946
ER -
TY - JOUR
AB - The inflammatory response to implants is a key stage of promoting
angiogenesis, wound healing and regulating osteogenesis. Macrophages play an
important role in inflammation due to their high plasticity. However, few reports
have focused on the effect of antibacterial materials on macrophage polarization.
In this work, micro/nano-structured titanium (MNT) was obtained through micro-arc
oxidization. We synthesized silver nanoparticles (AgNPs) with an average diameter
of 20 nm and immobilized AgNPs onto MNT via polydopamine to generate AgNP-decorated
MNT (AgPD-MNT). Further, we investigated the antibacterial activity of the
materials and the macrophage behaviors on the materials. The X-ray photoelectron
spectroscopy analysis indicated that metallic Ag and dopamine/polydopamine existed
on the AgPD-MNT surface. The concentration of released Ag was less than 8 ppb after
21 days. AgPD-MNT showed high antibacterial efficacy against Escherichia coli
without cytotoxicity toward osteoblasts. The macrophage proliferation and
morphology results showed that AgPD-MNT had no cytotoxicity within 5 days and
caused apoptosis after 7 days. The flow cytometry, enzyme-linked immuno sorbent
assay and quantitative real-time polymerase chain reaction results manifested that
the material groups (PD-MNT, MNT and AgPD-MNT) stimulated switching of macrophages
to mixed phenotypes (M1-M2) in the early stage (within 5 days), whereas the
expression of M2 macrophages was upregulated in the later stage. Among the material
groups, AgPD-MNT showed the most significant regulation of macrophage polarization.
Especially, AgPD-MNT induced adherent-macrophage apoptosis and upregulation of I
kappa B to reduce inflammation. Hence, AgPD-MNT could promote wound healing and
reduce the risk of implant-associated infection.
AN - WOS:000472230200006
AU - Li, Y. T.
AU - Yang, C. L.
AU - Yin, X. Z.
AU - Sun, Y. H.
AU - Weng, J.
AU - Zhou, J.
AU - Feng, B.
DA - JUN 14
DO - 10.1039/c8tb03245a
IS - 22
PY - 2019
SN - 2050-750X
2050-7518
SP - 3546-3559
ST - Inflammatory responses to micro/nano-structured titanium surfaces with silver
nanoparticles in vitro
TI - Inflammatory responses to micro/nano-structured titanium surfaces with silver
nanoparticles in vitro
VL - 7
ID - 6055
ER -
TY - JOUR
AB - The possibility that nanomaterials could perturb the normal course of an
inflammatory response is a key issue when assessing nanoimmunosafety. The
alteration of the normal progress of an inflammatory response may have pathological
consequences, since inflammation is a major defensive mechanism and its efficiency
maintains the body's health. The immunosafety of engineered nanoparticles at
nontoxic concentrations was investigated with the use of a human primary monocyte-
based in vitro system, which reproduces in a simplified fashion the full course of
the physiological inflammatory response, from initiation and development to
resolution. The kinetics of expression and production of inflammatory and anti-
inflammatory cytokines and the proteomic profiles were used for describing the
inflammatory defensive response. We assessed the ability of gold and silver
nanoparticles to trigger inflammation and to interfere with the course of an
ongoing defensive reaction. While neither nanoparticle type was able to directly
activate monocytes, silver nanoparticles could exacerbate the inflammatory response
of monocytes but did not interfere with the resolution of the inflammatory
reaction. These findings support the use of human primary monocyte-based in vitro
assays for realistically investigating the effects of engineered nanopartides on
human innate immune responses, in order to predict the immunological risk of
nanomaterials and implement safe nanoparticle-based applications.
AN - WOS:000386540300023
AU - Li, Y.
AU - Italiani, P.
AU - Casals, E.
AU - Valkenborg, D.
AU - Mertens, I.
AU - Baggerman, G.
AU - Nelissen, I.
AU - Puntes, V. F.
AU - Boraschi, D.
DA - OCT 26
DO - 10.1021/acsami.6b06278
IS - 42
PY - 2016
SN - 1944-8244
1944-8252
SP - 28437-28447
ST - Assessing the Immunosafety of Engineered Nanoparticles with a Novel in Vitro
Model Based on Human Primary Monocytes
TI - Assessing the Immunosafety of Engineered Nanoparticles with a Novel in Vitro
Model Based on Human Primary Monocytes
VL - 8
ID - 6174
ER -
TY - JOUR
AB - Due to the abuse of antibiotics and the emergence of multidrug resistant
microorganisms, medical devices, and related biomaterials are at high risk of
microbial infection during use, placing a heavy burden on patients and healthcare
systems. Metal-phenolic networks (MPNs), an emerging organic-inorganic hybrid
network system developed gradually in recent years, have exhibited excellent
multifunctional properties such as anti-inflammatory, antioxidant, and
antibacterial properties by making use of the coordination between phenolic ligands
and metal ions. Further, MPNs have received widespread attention in antimicrobial
infections due to their facile synthesis process, excellent biocompatibility, and
excellent antimicrobial properties brought about by polyphenols and metal ions. In
this review, different categories of biomaterials based on MPNs (nanoparticles,
coatings, capsules, hydrogels) and their fabrication strategies are summarized, and
recent research advances in their antimicrobial applications in biomedical fields
(e.g., skin repair, bone regeneration, medical devices, etc.) are highlighted.
AN - WOS:000830480100001
AU - Li, Y.
AU - Miao, Y.
AU - Yang, L. N.
AU - Zhao, Y. T.
AU - Wu, K. K.
AU - Lu, Z. H.
AU - Hu, Z. Q.
AU - Guo, J. S.
C6 - JUL 2022
C7 - 2202684
DA - SEP
DO - 10.1002/advs.202202684
IS - 27
PY - 2022
SN - 2198-3844
ST - Recent Advances in the Development and Antimicrobial Applications of Metal-
Phenolic Networks
T2 - ADVANCED SCIENCE
TI - Recent Advances in the Development and Antimicrobial Applications of Metal-
Phenolic Networks
VL - 9
ID - 6607
ER -
TY - JOUR
AB - Implant-associated infections are a major factor contributing to graft
failure. Preventing infection by inhibiting bacterial adhesion to implants is
critical for successful orthopedic surgery. In this work, we fabricated a novel
implant material and evaluated its performance in terms of inhibiting bacterial
growth and adhesion. Micro/nano-structured titanium (MNT) was prepared by the
micro-arc oxidization. Silver nanoparticles (AgNPs) with an average diameter of 10
nm were synthesized via a chemical reduction method and immobilized onto MNT to
generate an AgNP-decorated MNT (AgMN). It showed high antibacterial efficacy
against both Escherichia coli and Staphylococcus aureus without cytotoxicity to
NIH/3T3 fibroblast-like cells. AgMN implanted in rats inhibited S. aureus growth
and adhesion and elicited a milder inflammatory response than MNT. Moreover,
implanted AgMN had no adverse effect on the morphology and structure of attached
host cells after 1 day. These results indicate that using AgMN as an implant
material can reduce the risk of infection without toxic effects to the host.
Copyright © 2018 American Scientific Publishers All rights reserved.
AU - Li, Y.
AU - Tan, J.
AU - Liu, Z.
AU - Wang, G.
AU - Zhao, C.
AU - Duan, K.
AU - Weng, J.
AU - Feng, B.
DB - Scopus
DO - 10.1166/jbn.2018.2482
IS - 4
KW - Antibacterial
Cytotoxicity
Sliver nanoparticle
Titanium
Animals
Metal Nanoparticles
Mice
NIH 3T3 Cells
Rats
Silver
Adhesion
Cell culture
Enzyme inhibition
Escherichia coli
Metal nanoparticles
interleukin 6
nanomaterial
silver nanoparticle
titanium
titanium dioxide
antiinfective agent
metal nanoparticle
silver
Chemical reduction methods
Implant-associated infection
Morphology and structures
Sliver nanoparticles
animal experiment
Article
bacteriostasis
bacterium adherence
biocompatibility
cell structure
contact angle
controlled study
cytotoxicity
host cell
hydrodynamics
in vitro study
male
NIH 3T3 cell line
nonhuman
osseointegration
particle size
rat
animal
mouse
M3 - Article
PY - 2018
SP - 675-687
ST - Antibacterial activity and cyto/tissue-compatibility of micro/nano-structured
titanium decorated with silver nanoparticles
T2 - Journal of Biomedical Nanotechnology
TI - Antibacterial activity and cyto/tissue-compatibility of micro/nano-structured
titanium decorated with silver nanoparticles
85047620625&doi=10.1166%2fjbn.2018.2482&partnerID=40&md5=cddcac7583b404fd9615cf3b76
528aee
VL - 14
ID - 5508
ER -
TY - JOUR
AB - The inflammatory response to implants is a key stage of promoting
angiogenesis, wound healing and regulating osteogenesis. Macrophages play an
important role in inflammation due to their high plasticity. However, few reports
have focused on the effect of antibacterial materials on macrophage polarization.
In this work, micro/nano-structured titanium (MNT) was obtained through micro-arc
oxidization. We synthesized silver nanoparticles (AgNPs) with an average diameter
of 20 nm and immobilized AgNPs onto MNT via polydopamine to generate AgNP-decorated
MNT (AgPD-MNT). Further, we investigated the antibacterial activity of the
materials and the macrophage behaviors on the materials. The X-ray photoelectron
spectroscopy analysis indicated that metallic Ag and dopamine/polydopamine existed
on the AgPD-MNT surface. The concentration of released Ag was less than 8 ppb after
21 days. AgPD-MNT showed high antibacterial efficacy against Escherichia coli
without cytotoxicity toward osteoblasts. The macrophage proliferation and
morphology results showed that AgPD-MNT had no cytotoxicity within 5 days and
caused apoptosis after 7 days. The flow cytometry, enzyme-linked immuno sorbent
assay and quantitative real-time polymerase chain reaction results manifested that
the material groups (PD-MNT, MNT and AgPD-MNT) stimulated switching of macrophages
to mixed phenotypes (M1-M2) in the early stage (within 5 days), whereas the
expression of M2 macrophages was upregulated in the later stage. Among the material
groups, AgPD-MNT showed the most significant regulation of macrophage polarization.
Especially, AgPD-MNT induced adherent-macrophage apoptosis and upregulation of IκB
to reduce inflammation. Hence, AgPD-MNT could promote wound healing and reduce the
risk of implant-associated infection. © 2019 The Royal Society of Chemistry.
AU - Li, Y.
AU - Yang, C.
AU - Yin, X.
AU - Sun, Y.
AU - Weng, J.
AU - Zhou, J.
AU - Feng, B.
DB - Scopus
DO - 10.1039/c8tb03245a
IS - 22
KW - Amines
Cell death
Escherichia coli
Metal nanoparticles
Morphology
Pathology
Polarization
Titanium
Antibacterial efficacy
Antibacterial materials
Enzyme linked immunosorbent assay
Macrophage proliferation
Quantitative real-time polymerase chain reaction
Macrophages
M3 - Article
PY - 2019
SP - 3546-3559
ST - Inflammatory responses to micro/nano-structured titanium surfaces with silver
nanoparticles: In vitro
TI - Inflammatory responses to micro/nano-structured titanium surfaces with silver
nanoparticles: In vitro
85066878716&doi=10.1039%2fc8tb03245a&partnerID=40&md5=0591f1bbd677a32effcace66306bb
913
VL - 7
ID - 5383
ER -
TY - JOUR
AB - Due to the increasing applications of nanomaterials and nanotechnology,
potential danger of nanoparticle exposure has become a critical issue. However,
recent nanotoxicity studies have mainly focused on the health risks to healthy
adult population. The nanotoxicity effects on susceptible populations (such as
pregnant, neonate, diseased, and aged populations) have been overlooked. Due to the
alterations in physiological structures and functions in susceptible populations,
they often suffer more damage from the same exposure. Thus, it is urgent to
understand the effects of nanoparticle exposure on these populations. In order to
fill this gap, the potential effects of nanoparticles to pregnant females, neonate,
diseased, and aged population, as well as the possible underlying mechanisms are
reviewed in this article. Investigations show that responses from susceptible
population to nanoparticle exposure are often more severe. Reduced protection
mechanism, compromised immunity, and impaired self-repair ability in these
susceptible populations may contribute to the aggravated toxicity effects. This
review will help minimize adverse effects of nanoparticles to susceptible
population in future nanotechnology applications. © 2014 by the authors; licensee
AU - Li, Y.
AU - Zhang, Y.
AU - Yan, B.
DB - Scopus
DO - 10.3390/ijms15033671
IS - 3
KW - Aged population
Disease
Health risk
Nanotoxicity
Susceptible populations
Aged
Female
Humans
Infant, Newborn
Nanoparticles
Pregnancy
Pregnancy Complications
Respiration Disorders
Risk Assessment
Risk Factors
cadmium oxide
carbon black nanoparticle
carbon nanotube
chylomicron
gold nanoparticle
immunoglobulin E
immunoglobulin G1
interleukin 10
interleukin 13
interleukin 1beta
interleukin 4
interleukin 5
nanomaterial
nanoparticle
platelet derived growth factor AA
polystyrene
silver nanoparticle
titanium dioxide
unclassified drug
very low density lipoprotein
apoptosis
asthma
biocompatibility
chronic respiratory tract disease
DNA damage
embryo development
fetus development
fetus malformation
hepatitis
human
immunoreactivity
inflammation
nanotechnology
nanotoxicity
neurotoxicity
nonhuman
oxidative stress
phagocytosis
placenta disorder
pregnancy
protein degradation
review
thrombosis
toxicity
aged
female
newborn
procedures
risk assessment
risk factor
M3 - Review
PY - 2014
SP - 3671-3697
ST - Nanotoxicity overview: Nano-threat to susceptible populations
TI - Nanotoxicity overview: Nano-threat to susceptible populations
84895436939&doi=10.3390%2fijms15033671&partnerID=40&md5=3639ec01ab26287a34f815bd69e
57622
VL - 15
ID - 5601
ER -
TY - JOUR
AB - Ischemia-reperfusion (I/R) injury to the kidney, a major cause of acute renal
failure in humans, is associated with a high mortality, and the development of a
new therapeutic strategy is therefore highly desirable. In this study, we examined
the therapeutic potential of implantation of endothelial progenitor cells (EPCs)
isolated from Wharton's jelly of human umbilical cords in the treatment of renal
I/R injury in mice. To visualize the localization of the transplanted EPCs, the
cells were labeled with Q-tracker before injection into the renal capsule. Mice
with renal I/R injury showed a significant increase in blood urea nitrogen and
creatinine levels, and these effects were decreased by EPC transplantation. The
kidney injury score in the mice with I/R injury was also significantly decreased by
EPC transplantation. EPC transplantation increased the microvascular density, and
some of the EPCs surrounded and were incorporated into microvessels. In addition,
EPC transplantation inhibited the I/R-induced cell apoptosis of endothelial,
glomerular, and renal tubular cells, as demonstrated by TUNEL staining, and
significantly reduced reactive oxygen species production and the expression of the
inflammatory chemokines macrophage inflammatory protein-2 and keratinocyte-derived
cytokine, as shown by immunostain-ing and ELISA. Moreover, EPC transplantation
reduced I/R-induced fibrosis, as demonstrated by immuno-staining for S100A4, a
fibroblast marker, and by Jones silver staining. To our knowledge, this is the
first report that transplantation of EPCs from Wharton's jelly of human umbilical
cords might provide a novel therapy for ischemic acute kidney injury by promoting
angiogenesis and inhibiting apoptosis, inflammation, and fibrosis. © 2015 Cognizant
Comm. Corp.
AU - Liang, C. J.
AU - Shen, W. C.
AU - Chang, F. B.
AU - Wu, V. C.
AU - Wang, S. H.
AU - Young, G. H.
AU - Tsai, J. S.
AU - Tseng, Y. C.
AU - Peng, Y. S.
AU - Chen, Y. L.
DB - Scopus
DO - 10.3727/096368914X681720
IS - 7
KW - Acute kidney injury (AKI)
Angiogenesis
Apoptosis
Endothelial progenitor cells (EPCs)
Ischemia
Acute Kidney Injury
Animals
Endothelial Progenitor Cells
Fibrosis
Humans
Inflammation
Mice
Reperfusion Injury
Umbilical Cord
Wharton Jelly
Mus
calvasculin
chemokine
creatinine
cytokine
nitrogen
urea
animal experiment
animal model
animal tissue
apoptosis
Article
attenuation
blood sampling
cell culture
cell density
cell isolation
controlled study
endothelial progenitor cell
endothelial progenitor cell transplantation
glomerulus
human
human cell
in vitro study
in vivo study
inflammation
injection
Jones silver staining
keratinocyte
kidney blood flow
kidney capsule
kidney fibrosis
kidney function
kidney injury score
kidney ischemia
kidney parenchyma
kidney tubule cell
male
microvasculature
mouse
nonhuman
priority journal
protein expression
staining
TUNEL assay
umbilical cord
Wharton jelly
animal
fibrosis
metabolism
reperfusion injury
M3 - Article
PY - 2015
SP - 1363-1377
ST - Endothelial progenitor cells derived from wharton's jelly of human umbilical
cord attenuate ischemic acute kidney injury by increasing vascularization and
decreasing apoptosis, inflammation, and fibrosis
T2 - Cell Transplantation
TI - Endothelial progenitor cells derived from wharton's jelly of human umbilical
cord attenuate ischemic acute kidney injury by increasing vascularization and
decreasing apoptosis, inflammation, and fibrosis
84937403998&doi=10.3727%2f096368914X681720&partnerID=40&md5=551d64622089990c481d40f
73eab0cc4
VL - 24
ID - 5600
ER -
TY - JOUR
AB - Objective: This experiment was designed to demonstrate Mesenchymal stem cells
(MSCs) derived from kidney can alleviate cisplatin-induced kidney injury and renal
cell apoptosis through paracrine pathway. Methods: Firstly, MSCs were isolated from
kidney of young rats, and their surface-specific markers were identified by Reverse
Transcription-Polymerase Chain Reaction (RT-PCR) and immunofluorescence staining.
Self-renewal ability of Kidney Mesenchymal Stem Cells (KMSCs) was observed by cell
counting and 5-Bromo-2′-deoxyuridine (BrdU) fluorescence staining. KMSCs at
logarithmic growth stage were traced and injected into rat through tail vein.
Results: The results showed that KMSCs homed in the kidney tissues, decreased the
secretion of inflammatory factors (CRP, TNFα, IL-1β, IL-6), and alleviated renal
function. Hematoxylin and Eosin (H＆E), Masson and Periodic Acid-silver Methenamine
(PASM) staining showed that KMSCs could alleviate pathological damage in rats.
Terminal Deoxynucleotidyl Transferase mediated dUTP Nick-End Labeling (TUNEL) assay
showed that KMSCs could reduce the apoptosis of rat kidney cells induced by
cisplatin. Finally, Immunohistochemistry (IHC) results showed that cisplatin could
induce higher expression of the pro-apoptotic protein Bax and lower expression of
anti-apoptotic Bcl-2 in kidney tissues. However, KMSCs could reverse the pro-
apoptotic effect of cisplatin on kidney cells and improve the survival rate of
rats. Conclusions: In conclusion, KMSCs were successfully isolated from kidney
tissues, and KMSCs have therapeutic effects on rat kidney injury induced by
cisplatin. © 2022 The Authors
AU - Liang, R. N.
AU - Yan, D. Q.
AU - Zhang, X. P.
AU - Chen, X.
AU - Zhang, W. H.
AU - Jia, H. L.
C7 - 101998
DB - Scopus
DO - 10.1016/j.tice.2022.101998
KW - Apoptosis
Cisplatin
Inflammation
Kidney function
Kidney injury
Kidney Mesenchymal stem cells
Animals
Kidney
Rats
C reactive protein
cisplatin
interleukin 1beta
interleukin 6
protein Bax
protein bcl 2
animal experiment
animal model
animal tissue
apoptosis
Article
BrdU assay
cell proliferation
cell self-renewal
controlled study
fluorescence analysis
kidney cell
kidney function
kidney injury
kidney tissue
male
nonhuman
paracrine signaling
protein expression
rat
Sprague Dawley rat
survival rate
treatment outcome
TUNEL assay
animal
kidney
metabolism
M3 - Article
PY - 2023
ST - Kidney Mesenchymal stem cells alleviate cisplatin-induced kidney injury and
apoptosis in rats
T2 - Tissue and Cell
TI - Kidney Mesenchymal stem cells alleviate cisplatin-induced kidney injury and
apoptosis in rats
85144255220&doi=10.1016%2fj.tice.2022.101998&partnerID=40&md5=4a65056e651c21752c034
c816fb0a1e8
VL - 80
ID - 5040
ER -
TY - JOUR
AB - Diffuse axonal injury (DAI) is one of the most common and severe pathological
consequences of traumatic brain injury (TBI). The molecular mechanism of DAI is
highly complicated and still elusive, yet a clear understanding is crucial for the
diagnosis, treatment, and prognosis of DAI. In our study, we used rats to establish
a DAI model and applied isobaric tags for relative and absolute quantitation
(iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC–MS/MS)
analysis to identify differentially expressed proteins (DEPs) in the corpus
callosum. As a result, a total of 514 proteins showed differential expression
between the injury groups and the control. Among these DEPs, 14 common DEPs were
present at all seven time points postinjury (1, 3, 6, 12, 24, 48, and 72 h). Next,
bioinformatic analysis was performed to elucidate the pathogenesis of DAI, which
was found to possibly involve calcium ion–regulatory proteins (e.g., calsenilin and
ryanodine receptor 2), cytoskeleton organization (e.g., peripherin, NFL, NFM, and
NFH), apoptotic processes (e.g., calsenilin and protein kinase C delta type), and
inflammatory response proteins (e.g., complement C3 and C-reactive protein).
Moreover, peripherin and calsenilin were successfully confirmed by western blotting
to be significantly upregulated during DAI, and immunohistochemical (IHC) analysis
revealed that their expression increased and could be observed in axons after
injury, thus indicating their potential as DAI biomarkers. Our experiments not only
provide insight into the molecular mechanisms of axonal injury in rats during DAI
but also give clinicians and pathologists important reference data for the
diagnosis of DAI. Our findings may expand the list of DAI biomarkers and improve
the postmortem diagnostic rate of DAI. © 2019 Elsevier Inc.
AU - Liang, Y.
AU - Tong, F.
AU - Zhang, L.
AU - Zhu, L.
AU - Li, W.
AU - Huang, W.
AU - Zhao, S.
AU - He, G.
AU - Zhou, Y.
DB - Scopus
DO - 10.1016/j.brainresbull.2019.09.004
KW - Biomarkers
Diffuse axonal injury
iTRAQ
Proteomics
Rat
Animals
Axons
Brain
Brain Injuries, Traumatic
Chromatography, Liquid
Computational Biology
Corpus Callosum
Diffuse Axonal Injury
Female
Prognosis
Rats
Tandem Mass Spectrometry
biological marker
calcium ion
calpain
calsenilin
peripherin
regulator protein
ribonucleotide
Alzheimer disease
animal experiment
animal model
apoptosis
Article
axon
bioinformatics
biological phenomena and functions concerning the entire organism
brain stem
calcium signaling
cell fractionation
cell organelle
clinician
controlled study
corpus allatum
corpus callosum
cytoskeleton
cytosol
diffuse axonal injury
enzyme activation
extracellular space
hierarchical clustering
Huntington chorea
inflammation
mitochondrion
nonhuman
oxidative phosphorylation
Parkinson disease
pathogenesis
pathologist
pathophysiology
priority journal
prognosis
protein degradation
protein expression
protein protein interaction
proteomics
rat
silver staining
swelling
torsion
varicosis
Western blotting
white matter
animal
biology
brain
female
metabolism
pathology
procedures
Sprague Dawley rat
tandem mass spectrometry
M3 - Article
PY - 2019
SP - 289-304
ST - iTRAQ-based proteomic analysis discovers potential biomarkers of diffuse
axonal injury in rats
T2 - Brain Research Bulletin
TI - iTRAQ-based proteomic analysis discovers potential biomarkers of diffuse
85072765169&doi=10.1016%2fj.brainresbull.2019.09.004&partnerID=40&md5=cfc97dc8b62c7
60850f6338e9ee8f400
VL - 153
ID - 5363
ER -
TY - JOUR
AB - Background: The Lonicera japonica has been used as natural and healthy drink
for its anti-inflammatory effect and pleasant odor in China and Taiwan.Methods: 2D
electrophoresis was used to analyze the proteins involved in photoactivated
Lonicera japonica-induced CH27 cell apoptosis. The fluorescent dyes MitoTracker Red
CMXRos, calcein AM and JC-1 were used to elucidate mitochondrial function. The
protein expression was performed by Western blotting. Fluorescent image of
endoplasmic reticulum was accomplished by using ER-Tracker Green. This study used
fluorescent dye CM-H2DCFDA to detect intracellular generation of reactive oxygen
species.Results: The identified proteins can be classified into three major groups,
which include proteins involved in mitochondrial function, cytoskeleton-related
proteins and proteins associated with endoplasmic reticulum (ER) stress.
Photoactivated Lonicera japonica caused a significant effect on the mitochondrial
function and ER stress in CH27 cells. The reactive oxygen species producing was
found to be involved in photoactivated Lonicera japonica-induced CH27 cell
apoptosis.Conclusion: Mitochondria and endoplasmic reticulum are the integral
targets in photoactivated Lonicera japonica-induced CH27 cell apoptosis. We also
demonstrated that ethyl acetate fraction of Lonicera japonica extracts caused
photocytotoxicity in a dose-dependent manner in CH27 cells. This could explain the
fact that the ethyl acetate fraction of Lonicera japonica extracts may contain
compounds which exhibit the photosensitizing activity in CH27 cells. © 2013 Liao et
al.; licensee BioMed Central Ltd.
AU - Liao, J. C.
AU - Chang, W. T.
AU - Lan, Y. H.
AU - Hour, M. J.
AU - Lee, H. Z.
C7 - 244
DB - Scopus
DO - 10.1186/1472-6882-13-244
KW - 2D electrophoresis
Endoplasmic reticulum chaperones
Human lung squamous carcinoma CH27 cells
Lonicera japonica
Mitochondrial chaperones
Photocytotoxicity
Cell Line, Tumor
Cell Survival
Humans
Lonicera
Lung Neoplasms
Mitochondria
Molecular Chaperones
Oxidative Stress
Photochemical Processes
Plant Extracts
Proteome
Proteomics
calcein
chaperone
fluorescent dye
Lonicera japonica extract
apoptosis
article
controlled study
cytoskeleton
endoplasmic reticulum
lung squamous cell carcinoma
mitochondrion
nonhuman
nucleotide sequence
photoactivation
photosensitization
phototoxicity
protein analysis
protein expression
proteomics
silver staining
Western blotting
M3 - Article
PY - 2013
ST - Application of proteomics to identify the target molecules involved in
Lonicera japonica-induced photokilling in human lung cancer CH27 cells
TI - Application of proteomics to identify the target molecules involved in
Lonicera japonica-induced photokilling in human lung cancer CH27 cells
84884970889&doi=10.1186%2f1472-6882-13-
244&partnerID=40&md5=e8f408336ceef5b56517da95d937f2bb
VL - 13
ID - 5741
ER -
TY - JOUR
AB - Detection of chemical reactions in living cells is critical in understanding
physiological metabolic processes in the context of nanomedicine. Carbon monoxide
(CO) is one of the important gaseous signaling molecules. Surface-enhanced Raman
spectroscopy (SERS)-based CO-releasing nanoparticles (CORN) is utilized to
investigate the chemical reaction of CO delivery in live cells. Using SERS CORN,
carbonyl dissociation from CORN-Ag-CpW(CO)3to CORN-Ag-CpW(CO)2in live cells is
observed. The subsequent irreversible degradation to CO-free CORN is a consequence
of oxidative stress in cells. This observation affirms the step transition of CORN-
Ag-CpW(CO)3in cellular: CORN-Ag-CpW(CO)3first proceedsviaa direct loss of one CO
followed by a oxidative decomposition giving rise to CORN-Ag-WO3and as well as the
release of one equivalents of CO. Importantly, the decarbonylation process can be
correlated with the level of inflammatory biomarkers. For the first time, we
provide unambiguous evidence for the steps transition of CO-release mechanism in
cellular. © The Royal Society of Chemistry 2020.
AU - Liao, P. H.
AU - Tseng, C. Y.
AU - Ke, Z. Y.
AU - Hsieh, C. L.
AU - Kong, K. V.
DB - Scopus
DO - 10.1039/d0cc01297a
IS - 35
KW - Animals
Carbon Monoxide
Cell Line
Cytokines
Humans
Metal Nanoparticles
Mice
Silver
Spectrum Analysis, Raman
Tungsten
Carbon monoxide
Cells
Chemical detection
Degradation
Raman spectroscopy
carbon monoxide
carbon monoxide releasing nanoparticle
carbonyl derivative
doxorubicin
interleukin 1beta
interleukin 6
nanoparticle
silver
tungsten derivative
tungsten tricarbonyl complex
unclassified drug
cytokine
metal nanoparticle
tungsten
Detection of chemicals
Irreversible degradation
Metabolic process
Oxidative decomposition
Release mechanism
Signaling molecules
Step transitions
Surface enhanced Raman spectroscopy
animal cell
Article
cell viability
chemical bond
chemical reaction
controlled study
cytokine release
cytotoxicity
decarbonylation
dissociation
fluorescence imaging
human
human cell
mouse
nonhuman
oxidation
oxidative decomposition
oxidative stress
Raman spectrometry
RAW 264.7 cell line
skin fibroblast
animal
cell line
metabolism
Cytology
M3 - Article
PY - 2020
SP - 4852-4855
ST - Operando characterization of chemical reactions in single living cells using
SERS
T2 - Chemical Communications
TI - Operando characterization of chemical reactions in single living cells using
SERS
85084192850&doi=10.1039%2fd0cc01297a&partnerID=40&md5=406183ea810a7ee0246ee2b292409
c62
VL - 56
ID - 5332
ER -
TY - JOUR
AB - Urinary tract infections accounts for over 40% of all nosocomial infections,
and almost all these infections are associated with indwelling catheters. The
acquisition of urinary tract infections following urinary bladder catheterizations
are associated with nearly a threefold increase in mortality among hospitalized
patients. The economic impact of nosocomial urinary infections is difficult to
assess. An estimate of the cost of these infections have shown that patients with
hospital-acquired urinary tract infections secondary to indwelling catheters, spent
an average of 2.4 additional days in the hospital. Bearing this in mind, even a
marginal decrease in urinary tract infections may be cost-effective. In two
randomized prospective clinical studies we have shown that coating urinary
catheters with silver significantly reduces the infection rate during short-term
catheterization (less than 7 days). We also showed that silver coating of urinary
catheters prevented adherence and growth of Pseudomonas aeruginosa on the catheter
material. Another risk from using indwelling catheters is an inflammatory reaction
of the urethral mucosa leading to stricture formation. Several aetiological factors
whereby indwelling catheters may cause a urethral stricture have been discussed.
During the last years much attention has been paid to the catheter material as
such, especially latex, and its role in stricture formation. Urinary catheters are
made from a variety of materials combined with different chemicals. It seems as if
these chemical substances can dissolve from the catheter material, causing
inflammatory reactions. Using a cell culture technique with a mouse fibroblast cell
line (BALBc/3T3), and an animal model with implantation of catheter material into
the urethra, we assessed both in vitro cytotoxicity (IC50) and inflammatory
reactions in vivo from different catheter materials. The studies confirmed that
especially latex materials do not have both cytotoxic effects and cause
considerable inflammation within the urethral mucosa. By coating the catheters with
silver, the cytotoxicity could be significantly reduced as compared with pure latex
and hydrogel coated latex catheters. Several studies have demonstrated a cytotoxic
effect from catheter materials, indicating that this may be of importance in
urethral inflammation. However, the exact mechanisms behind this phenomenon is not
known. In an attempt to explain the inflammatory reaction within the urethra
secondary to an indwelling catheter, we investigated the influence of the nervous
system on experimentally induced urethral inflammation. Our findings indicate that
an important part in catheter induced inflammation is played by neurogenic
reactions.
AU - Liedberg, H.
DB - Scopus
KW - Animals
Bacterial Adhesion
Cross Infection
Female
Humans
Hydrogel
Latex
Polytetrafluoroethylene
Rats
Silicones
Silver
Urethritis
Urinary Catheterization
Urinary Tract Infections
latex
macrogol derivative
politef
silicone derivative
silver
animal
article
bacterium adherence
catheterization
cross infection
female
human
hydrogel
instrumentation
rat
rat strain
urethritis
M3 - Article
PY - 1989
SP - 1-43
ST - Catheter induced urethral inflammatory reaction and urinary tract infection.
An experimental and clinical study
T2 - Scandinavian journal of urology and nephrology. Supplementum
TI - Catheter induced urethral inflammatory reaction and urinary tract infection.
An experimental and clinical study
0024809594&partnerID=40&md5=6c6caeadfb5a98801a649e3a8f458ad0
VL - 124
ID - 5818
ER -
TY - JOUR
AB - Because of the limited information on size-dependent particle-mediated
effects, the present study was conducted to determine if the changes in induced
protein expression between 5 nm silver nanoparticles and 100 nm particles after
exposure to sub-lethal concentrations. A total of 28,000 cDNA profiles were
screened using 5 nm silver nanoparticles and 100 nm silver nanoparticles in a
macrophage cell line. Based on results obtained from cDNA microarray we also
assessed protein levels of hemeoxygenase-1 (HO-1), heat shock protein-70 (HSP-70)
and interleukin-8 (IL-8), which were shown to significantly increase. Together with
results obtained using N-acetylcystein (NAC), we were able to clearly show that low
level and early stage exposure to 5 nm silver nanoparticles, but not 100 nm,
induces expression of IL-8 as well as stress genes against reactive oxygen species
(ROS). Therefore, we provide important data to understand and identify the early
effects of silver nanoparticles on the immune system. (C) 2012 Elsevier Ltd. All
rights reserved.
AN - WOS:000303953000027
AU - Lim, D. H.
AU - Jang, J.
AU - Kim, S.
AU - Kang, T.
AU - Lee, K.
AU - Choi, I. H.
DA - JUN
IS - 18
PY - 2012
SN - 0142-9612
1878-5905
SP - 4690-4699
ST - The effects of sub-lethal concentrations of silver nanoparticles on
inflammatory and stress genes in human macrophages using cDNA microarray analysis
T2 - BIOMATERIALS
TI - The effects of sub-lethal concentrations of silver nanoparticles on
inflammatory and stress genes in human macrophages using cDNA microarray analysis
VL - 33
ID - 6025
ER -
TY - JOUR
AB - Bioactive glasses (BG) applications include tissue engineering for bone
regeneration, coating for implants, and scaffolds for wound healing. BG can be
conjugated to ions like silver, which might add some antimicrobial properties to
this biomaterial. The immunomodulatory activity of ion-doped bioactive glasses
particles was not investigated before. The aim of this work was to evaluate the
cytotoxic and immunomodulatory effect of BG and silver-doped bioactive glass (BGAg)
in human peripheral blood cells. BG and BGAg samples belonging to the system 58SiO2
•(36-x)CaO·6P2O5 ·xAg2O, where x = 0 and 1 mol%, respectively, were synthesized via
sol-gel method and characterized. Cytotoxicity, modulation of cytokine production
(TNF-α, IL-1β, IL-6, IL-4, and IL-10), and oxidative stress response were
investigated in human polymorphonuclear cells (PMNs) and peripheral blood
mononuclear cells (PBMCs) cultures. Cell viability in the presence of BG or BGAg
was concentration-dependent. In addition, BGAg presented higher PBMCs toxicity
(LC50 = 0.005%) when compared to BG (LC50 = 0.106%). Interestingly, interleukin4
was produced by PBMCs in response to BG and BGAg in absence of phytohemagglutinin
(PHA) and did not modulate PHA-induced cytokine levels. Subtoxic concentrations
(0.031% for BG and 0.0008% for BGAg) did not change other cytokines in PBMCs nor
reactive oxygen species (ROS) production by PMN. However, BG and BGAg particles
decreased zymosan-induced ROS levels in PMN. Although ion incorporation increased
BG cytotoxicity, the bioactive glass particles demonstrated a in vitro anti-
inflammatory potencial. Future studies are needed to clarify the scavenger
potential of the BG/BGAg particles/scaffolds as well as elucidate the effect of the
anti-inflammatory potential in modulating tissue growth in vivo. © 2019 Jefferson
Muniz de Lima et al.
AU - Lima, J. M. D.
AU - Pinheiro Ferreira, E.
AU - Bonan, R. F.
AU - Silva-Teixeira, D. N.
AU - Goulart, L. R.
AU - Souza, J. R. D.
AU - De Medeiros, E. S.
AU - Bonan, P. R. F.
AU - Castellano, L. R. C.
C7 - 3210530
DB - Scopus
DO - 10.1155/2019/3210530
KW - Biocompatible Materials
Cell Line
Cell Survival
Cytokines
Glass
Humans
Inflammation
Silver
Tissue Engineering
Wound Healing
cytokine
glass
interleukin 10
interleukin 1beta
interleukin 4
interleukin 6
phytohemagglutinin
silver
biomaterial
Article
cell culture
cell viability
cytokine production
cytotoxicity
human
human cell
in vitro study
leukocyte
oxidative stress
tissue growth
cell line
cell survival
chemistry
drug effect
inflammation
metabolism
mononuclear cell
procedures
tissue engineering
wound healing
M3 - Article
PY - 2019
ST - Cytokine Regulation from Human Peripheral Blood Leukocytes Cultured in Vitro
TI - Cytokine Regulation from Human Peripheral Blood Leukocytes Cultured in Vitro
85068256873&doi=10.1155%2f2019%2f3210530&partnerID=40&md5=72c17953708ed72b38cad69f0
2dc7a29
VL - 2019
ID - 5374
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) enter the central nervous system through the
blood-brain barrier (BBB). AgNP exposure canincrease amyloid beta (Aβ) deposition
in neuronal cells to potentially induce Alzheimer's disease (AD)
progression.However, the mechanism through which AgNPs alter BBB permeability in
endothelial cells and subsequently lead toAD progression remains unclear. This
study investigated whether AgNPs disrupt the tight junction proteins of
brainendothelial cells, and alter the proteomic metabolismof neuronal cells
underlying AD progression in a triple cell coculturemodel constructed using mouse
brain endothelial (bEnd.3) cells, mouse brain astrocytes (ALT), and mouse
neuroblastomaneuro-2a (N2a) cells. The results showed that AgNPs accumulated in ALT
and N2a cells because of the disruption of tightjunction proteins, claudin-5 and
ZO-1, in bEnd.3 cells. The proteomic profiling of N2a cells after AgNP exposure
identified298 differentially expressed proteins related to fatty acid metabolism.
Particularly, AgNP-induced palmitic acid productionwas observed in N2a cells, which
might promote Aβ generation. Moreover, AgNP exposure increased the protein
expressionof amyloid precursor protein (APP) and Ab generation-related secretases,
PSEN1, PSEN2, and b-site APP cleaving enzyme forAPP cleavage in ALT and N2a cells,
stimulated Aβ40 and Aβ42 secretion in the culture medium, and attenuated the
geneexpression of Aβ clearance-related receptors, P-gp and LRP-1, in bEnd.3 cells.
Increased Aβ might further aggregate on theneuronal cell surface to enhance the
secretion of inflammatory cytokines, MCP-1 and IL-6, thus inducing apoptosis in
N2acells. This study suggested that AgNP exposure might cause Aβ deposition and
inflammation for subsequent neuronal cellapoptosis to potentially induce AD
progression. © The Author 2017. Published by Oxford University Press on behalf of
AU - Lin, H. C.
AU - Ho, M. Y.
AU - Tsen, C. M.
AU - Huang, C. C.
AU - Wu, C. C.
AU - Huang, Y. J.
AU - Hsiao, I. L.
AU - Chuang, C. Y.
C7 - kfx079
DB - Scopus
DO - 10.1093/toxsci/kfx079
IS - 1
KW - Alzheimer's disease
Amyloid beta clearance
Blood-brain barrier
Proteomic profiling
Tight junction protein
Amyloid beta-Peptides
Animals
Apoptosis
Astrocytes
Blood-Brain Barrier
Cytokines
Evans Blue
Fatty Acids
Metal Nanoparticles
Mice
Models, Biological
Neurons
Proteomics
Silver
Tight Junction Proteins
amyloid beta protein[1-40]
beta secretase
claudin 5
fatty acid
interleukin 6
protein ZO1
silver nanoparticle
autacoid
cytokine
Evans blue
metal nanoparticle
silver
Alzheimer disease
animal cell
apoptosis
Article
astrocyte
blood brain barrier
coculture
controlled study
cytokine release
fatty acid analysis
fatty acid metabolism
gene expression
inflammation
mouse
nerve cell
neuroblastoma cell
nonhuman
protein expression
protein metabolism
proteomics
tight junction
animal
biological model
chemistry
comparative study
cytology
drug effect
metabolism
secretion (process)
M3 - Article
PY - 2017
SP - 151-163
ST - Comparative proteomics reveals silver nanoparticles alter fatty acid
metabolism and amyloid beta clearance for neuronal apoptosis in a triple cell
coculture model of the blood-brain barrier
TI - Comparative proteomics reveals silver nanoparticles alter fatty acid
metabolism and amyloid beta clearance for neuronal apoptosis in a triple cell
coculture model of the blood-brain barrier
85022338014&doi=10.1093%2ftoxsci
%2fkfx079&partnerID=40&md5=54f3ee55693e89c05ad8ba5e85d20a5b
VL - 158
ID - 5488
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) enter the central nervous system through the
blood-brain barrier (BBB). AgNP exposure can increase amyloid beta (A beta)
deposition in neuronal cells to potentially induce Alzheimer's disease (AD)
progression. However, the mechanism through which AgNPs alter BBB permeability in
endothelial cells and subsequently lead to AD progression remains unclear. This
study investigated whether AgNPs disrupt the tight junction proteins of brain
endothelial cells, and alter the proteomic metabolism of neuronal cells underlying
AD progression in a triple cell coculture model constructed using mouse brain
endothelial (bEnd.3) cells, mouse brain astrocytes (ALT), and mouse neuroblastoma
neuro-2a (N2a) cells. The results showed that AgNPs accumulated in ALT and N2a
cells because of the disruption of tight junction proteins, claudin-5 and ZO-1, in
bEnd.3 cells. The proteomic profiling of N2a cells after AgNP exposure identified
298 differentially expressed proteins related to fatty acid metabolism.
Particularly, AgNP-induced palmitic acid production was observed in N2a cells,
which might promote A beta generation. Moreover, AgNP exposure increased the
protein expression of amyloid precursor protein (APP) and A beta generation-related
secretases, PSEN1, PSEN2, and beta-site APP cleaving enzyme for APP cleavage in ALT
and N2a cells, stimulated A beta 40 and A beta 42 secretion in the culture medium,
and attenuated the gene expression of A beta clearance-related receptors, P-gp and
LRP-1, in bEnd.3 cells. Increased A beta might further aggregate on the neuronal
cell surface to enhance the secretion of inflammatory cytokines, MCP-1 and IL-6,
thus inducing apoptosis in N2a cells. This study suggested that AgNP exposure might
cause A beta deposition and inflammation for subsequent neuronal cell apoptosis to
potentially induce AD progression.
AN - WOS:000405698800013
AU - Lin, H. C.
AU - Ho, M. Y.
AU - Tsen, C. M.
AU - Huang, C. C.
AU - Wu, C. C.
AU - Huang, Y. J.
AU - Hsiao, I. L.
AU - Chuang, C. Y.
DA - JUL
DO - 10.1093/toxsci/kfx079
IS - 1
PY - 2017
SN - 1096-6080
1096-0929
SP - 151-163
ST - From the Cover: Comparative Proteomics Reveals Silver Nanoparticles Alter
Fatty Acid Metabolism and Amyloid Beta Clearance for Neuronal Apoptosis in a Triple
Cell Coculture Model of the Blood-Brain Barrier
T2 - TOXICOLOGICAL SCIENCES
TI - From the Cover: Comparative Proteomics Reveals Silver Nanoparticles Alter
Fatty Acid Metabolism and Amyloid Beta Clearance for Neuronal Apoptosis in a Triple
Cell Coculture Model of the Blood-Brain Barrier
VL - 158
ID - 6043
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are commonly used in daily living products.
AgNPs can induce inflammatory response in neuronal cells, and potentially develop
neurological disorders. The gene networks in response to AgNPs-induced
neurodegenerative progression have not been clarified in various brain neural
cells. This study found that 3-5 nm AgNPs were detectable to enter the nuclei of
mouse neuronal cells after 24-h of exposure. The differentially expressed genes in
mouse brain neural cells exposure to AgNPs were further identified using Phalanx
Mouse OneArray® chip, and permitted to explore the gene network pathway regulating
in neurodegenerative progression according to Cytoscape analysis. In focal adhesion
pathway of ALT astrocytes, AgNPs induced the gene expression of RasGRF1 and reduced
its downstream BCL2 gene for apoptosis. In cytosolic DNA sensing pathway of
microglial BV2 cells, AgNPs reduced the gene expression of TREX1 and decreased IRF7
to release pro-inflammatory cytokines for inflammation and cellular activation. In
MAPK pathway of neuronal N2a cells, AgNPs elevated GADD45α gene expression, and
attenuated its downstream PTPRR gene to interfere with neuron growth and
differentiation. Moreover, AgNPs induced beta amyloid deposition in N2a cells, and
decreased PSEN1 and PSEN2, which may disrupt calcium homeostasis and presynaptic
dysfunction for Alzheimer's disease development. These findings suggested that
AgNPs exposure reveals the potency to induce the progression of neurodegenerative
disorder. © 2016 Elsevier B.V.
AU - Lin, H. C.
AU - Huang, C. L.
AU - Huang, Y. J.
AU - Hsiao, I. L.
AU - Yang, C. W.
AU - Chuang, C. Y.
DB - Scopus
DO - 10.1016/j.tiv.2016.04.014
KW - Alzheimer's disease
Beta amyloid
Gene network
Neurodegenerative disease
Silver nanoparticle
Amyloid beta-Peptides
Animals
Astrocytes
Brain
Cell Line
Cell Line, Tumor
Gene Expression
Metal Nanoparticles
Mice
Microglia
Mitogen-Activated Protein Kinases
Neurons
Presenilin-1
Presenilin-2
Silver
guanine nucleotide exchange factor
interferon regulatory factor 7
protein bcl 2
silver nanoparticle
metal nanoparticle
presenilin 1
presenilin 1, mouse
presenilin 2
Psen2 protein, mouse
silver
animal cell
apoptosis
Article
BCL2 gene
controlled study
cytokine release
GADD45 alpha gene
gene
gene expression
genetic analysis
genetic association
IRF7 gene
microglia
mouse
nerve cell
nerve degeneration
nonhuman
RasGRF1 gene
transcriptomics
TREX1 gene
animal
astrocyte
brain
cell line
cytology
drug effects
genetics
metabolism
tumor cell line
M3 - Article
PY - 2016
SP - 289-299
ST - Transcriptomic gene-network analysis of exposure to silver nanoparticle
reveals potentially neurodegenerative progression in mouse brain neural cells
TI - Transcriptomic gene-network analysis of exposure to silver nanoparticle
reveals potentially neurodegenerative progression in mouse brain neural cells
84968901983&doi=10.1016%2fj.tiv.2016.04.014&partnerID=40&md5=7f22f677ecb79770ab4382
aff07ae2a2
VL - 34
ID - 5478
ER -
TY - JOUR
AB - Inflenza A viruses (IAVs) are highly transmissible and pathogenic
Orthomyxoviruses, which have led to worldwide outbreaks and seasonal pandemics of
acute respiratory diseases, causing serious threats to public health. Currently
used anti-influenza drugs may cause neurological side effects, and they are
increasingly less effective against mutant strains. To help prevent the spread of
IAVs, in this work, we have developed quercetin-derived carbonized nanogels
(CNGsQur) that display potent viral inhibitory, antioxidative, and anti-
inflammatory activities. The antiviral CNGsQur were synthesized by mild
carbonization of quercetin (Qur), which successfully preserved their antioxidative
and anti-inflammatory properties while also contributed enhanced properties, such
as water solubility, viral binding, and biocompatibility. Antiviral assays of co-
treatment, pre-treatment, and post-treatment indicate that CNGsQur interacts with
the virion, revealing that the major antiviral mechanism resulting in the
inhibition of the virus is by their attachment on the cell surface. Among them, the
selectivity index (SI) of CNGsQur270 (>857.1) clearly indicated its great potential
for clinical application in IAVs inhibition, which was much higher than that of
pristine quercetin (63.7) and other clinical drugs (4-81). Compared with quercetin
at the same dose, the combined effects of viral inhibition, antioxidative and anti-
inflammatory activities impart the superior therapeutic effects of CNGsQur270
aerosol inhalation in the treatment of IAVs infection, as evidenced by a mouse
model. These CNGsQur effectively prevent the spread of IAVs and suppress virus -
induced inflammation while also exhibiting good in vivo biocompatibility. CNGsQur
shows much promise as a clinical therapeutic agent against infection by IVAs. (c)
2022 Elsevier Inc. All rights reserved.
AN - WOS:000798835900010
AU - Lin, H. Y.
AU - Zeng, Y. T.
AU - Lin, C. J.
AU - Harroun, S. G.
AU - Anand, A.
AU - Chang, L.
AU - Wu, C. J.
AU - Lin, H. J.
AU - Huang, C. C.
C6 - MAY 2022
DA - SEP 15
DO - 10.1016/j.jcis.2022.04.124
PY - 2022
SN - 0021-9797
1095-7103
SP - 481-493
ST - Partial carbonization of quercetin boosts the antiviral activity against H1N1
influenza A virus
TI - Partial carbonization of quercetin boosts the antiviral activity against H1N1
influenza A virus
VL - 622
ID - 6395
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are known for their excellent antibacterial
activities. The possible toxicity, however, is a major concern for their
applications. Three types of AgNPs were prepared in this study by chemical
processes. Each was stabilized by a polymer surfactant, which was expected to
reduce the exposure of cells to AgNPs and therefore their cytotoxicity. The polymer
stabilizers included poly(oxyethylene)-segmented imide (POEM), poly(styrene-co-
maleic anhydride)-grafting poly(oxyalkylene) (SMA) and poly(vinyl alcohol) (PVA).
The cytotoxicity of these chemically produced AgNPs to mouse skin fibroblasts
(L929), human hepatocarcinoma cells (HepG2), and mouse monocyte macrophages
(J774A1) was compared to that of physically produced AgNPs and gold nanoparticles
(AuNPs) as well as the standard reference material RM8011 AuNPs. Results showed
that SMA-AgNPs were the least cytotoxic among all materials, but cytotoxicity was
still observed at higher silver concentrations (>30ppm). Macrophages demonstrated
the inflammatory response with cell size increase and viability decrease upon
exposure to 10ppm of the chemically produced AgNPs. SMA-AgNPs did not induce
hemolysis at a silver concentration below 1.5ppm. Regarding the antibacterial
activity, POEM-AgNPs and SMA-AgNPs at 1ppm silver content showed 99.9% and 99.3%
growth inhibition against E. coli, while PVA-AgNPs at the same silver concentration
displayed 79.1% inhibition. Overall, SMA-AgNPs demonstrated better safety in vitro
and greater antibacterial effects than POEM-AgNPs and PVA-AgNPs. This study
suggested that polymer stabilizers may play an important role in determining the
toxicity of AgNPs. © 2012 IOP Publishing Ltd.
AU - Lin, J. J.
AU - Lin, W. C.
AU - Dong, R. X.
AU - Hsu, S. H.
C7 - 065102
DB - Scopus
DO - 10.1088/0957-4484/23/6/065102
IS - 6
KW - Animals
Cell Line
Cell Survival
Escherichia coli
Escherichia coli Infections
Humans
Maleates
Mice
Nanoparticles
Polymers
Polystyrenes
Polyvinyl Alcohol
Silver
Surface-Active Agents
Cell culture
Cytotoxicity
Macrophages
Maleic anhydride
Mammals
Polyethylene oxides
Silver metallurgy
Styrene
Toxicity
antiinfective agent
macrogol derivative
maleic acid derivative
nanoparticle
poly(styrene co maleic anhydride)
poly(styrene-co-maleic anhydride)
polymer
polystyrene derivative
polyvinyl alcohol
silver
surfactant
Cell size
Cellular response
Chemical process
Cytotoxic
E. coli
Gold nanoparticle
Growth inhibition
Human hepatocarcinoma
In-vitro
Monocyte-macrophages
Mouse skin
Poly(oxyalkylene)
Poly(oxyethylene)
Polymer Stabilizer
Polymer surfactants
Silver concentration
Silver content
Standard reference material
animal
article
cell line
cell survival
chemistry
drug effect
Escherichia coli infection
human
mouse
ultrastructure
M3 - Article
PY - 2012
ST - The cellular responses and antibacterial activities of silver nanoparticles
stabilized by different polymers
T2 - Nanotechnology
TI - The cellular responses and antibacterial activities of silver nanoparticles
stabilized by different polymers
84855944209&doi=10.1088%2f0957-
4484%2f23%2f6%2f065102&partnerID=40&md5=b637ae7affcc09f9481cd013cd041d56
VL - 23
ID - 5689
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are known for their bactericidal abilities. The
antibacterial potency is dependent on the particle size and dispersion status. In
this study, we synthesized AgNP/NSP nanohybrids in two different weight ratios
(1/99 and 8/92) using the fully exfoliated clay, i.e., nanosilicate platelets
(NSP), as a dispersing agent and carrier for AgNPs. Due to the size of NSP, the
immobilized AgNPs do not enter cells readily, which may lower the risk associated
with the cellular uptake of AgNPs. The biocompatibility, immunological response,
and antimicrobial activities of AgNP/NSP hybrids were evaluated. The results
revealed that AgNP/NSP hybrids elicited merely mild inflammatory response and
retained the outstanding antibacterial activity. The hybrids were further embedded
in poly(ether)urethane (PEU) to increase the biocompatibility. At the same silver
content (20 ppm), the PEU-AgNP/NSP nanocomposites were nontoxic to mouse skin
fibroblasts, while simultaneously exhibiting nearly complete bacterial growth
reduction (99.9%). PEU containing the same amount of free AgNPs did not display
such an effect. Our results verify the better biosafety of the AgNPs/NSP hybrids
and their polymer nanocomposites for further clinical use. © 2012 American Chemical
Society.
AU - Lin, J. J.
AU - Lin, W. C.
AU - Li, S. D.
AU - Lin, C. Y.
AU - Hsu, S. H.
DB - Scopus
DO - 10.1021/am302534k
IS - 2
biocompatibility
nanocomposites
nanosilicate platelets (NSP)
polyurethane
silver nanoparticles (AgNPs)
Animals
Cell Line
Drug Carriers
Escherichia coli
Humans
Mice
Nanocomposites
Nanoparticles
Particle Size
Silicates
Silver
Biocompatibility
Cell culture
Dispersions
Ethers
Platelets
Polyurethanes
antiinfective agent
drug carrier
nanocomposite
nanoparticle
silicate
silver
Bacterial growth
Bactericidal ability
Cellular uptake
Clinical use
Dispersing agent
Exfoliated clay
Mouse skin
Nanohybrids
Silicate platelets
Silver content
Weight ratios
animal
article
cell line
chemistry
drug effect
evaluation
human
methodology
mouse
particle size
M3 - Article
PY - 2013
SP - 433-443
ST - Evaluation of the antibacterial activity and biocompatibility for silver
nanoparticles immobilized on nano silicate platelets
TI - Evaluation of the antibacterial activity and biocompatibility for silver
nanoparticles immobilized on nano silicate platelets
84872871990&doi=10.1021%2fam302534k&partnerID=40&md5=a982b8d64d078f465da14ce6e1ae34
62
VL - 5
ID - 5756
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) possess anti-inflammatory activities and have
been widely deployed for promoting tissue repair. Here we explored the efficacy of
AgNPs on functional recovery after spinal cord injury (SCI). Our data indicated
that, in a SCI rat model, local AgNPs delivery could significantly recover
locomotor function and exert neuroprotection through reducing of pro-inflammatory
M1 survival. Furthermore, in comparison with Raw 264.7-derived M0 and M2, a higher
level of AgNPs uptake and more pronounced cytotoxicity were detected in M1. RNA-seq
analysis revealed the apoptotic genes in M1 were upregulated by AgNPs, whereas in
M0 and M2, pro-apoptotic genes were downregulated and PI3k-Akt pathway signaling
pathway was upregulated. Moreover, AgNPs treatment preferentially reduced cell
viability of human monocyte-derived M1 comparing to M2, supporting its effect on M1
in human. Overall, our findings reveal AgNPs could suppress M1 activity and imply
its therapeutic potential in promoting post-SCI motor recovery.
AN - WOS:001026401700001
AU - Lin, J.
AU - Chen, P. K.
AU - Tan, Z. J.
AU - Sun, Y.
AU - Tam, W. K.
AU - Ao, D.
AU - Shen, W.
AU - Leung, V. Y. L.
AU - Cheung, K. M. C.
AU - To, M. K. T.
C7 - e15689
DA - MAY
DO - 10.1016/j.heliyon.2023.e15689
IS - 5
PY - 2023
SN - 2405-8440
ST - Application of silver nanoparticles for improving motor recovery after spinal
cord injury via reduction of pro-inflammatory M1 macrophages
T2 - HELIYON
TI - Application of silver nanoparticles for improving motor recovery after spinal
VL - 9
ID - 6004
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) possess anti-inflammatory activities and have
been widely deployed for promoting tissue repair. Here we explored the efficacy of
AgNPs on functional recovery after spinal cord injury (SCI). Our data indicated
that, in a SCI rat model, local AgNPs delivery could significantly recover
locomotor function and exert neuroprotection through reducing of pro-inflammatory
M1 survival. Furthermore, in comparison with Raw 264.7-derived M0 and M2, a higher
level of AgNPs uptake and more pronounced cytotoxicity were detected in M1. RNA-seq
analysis revealed the apoptotic genes in M1 were upregulated by AgNPs, whereas in
M0 and M2, pro-apoptotic genes were downregulated and PI3k-Akt pathway signaling
pathway was upregulated. Moreover, AgNPs treatment preferentially reduced cell
viability of human monocyte-derived M1 comparing to M2, supporting its effect on M1
in human. Overall, our findings reveal AgNPs could suppress M1 activity and imply
its therapeutic potential in promoting post-SCI motor recovery. © 2023 The
Author(s)
AU - Lin, J.
AU - Chen, P.
AU - Tan, Z.
AU - Sun, Y.
AU - Tam, W. K.
AU - Ao, D.
AU - Shen, W.
AU - Leung, V. Y. L.
AU - Cheung, K. M. C.
AU - To, M. K. T.
C7 - e15689
DB - Scopus
DO - 10.1016/j.heliyon.2023.e15689
IS - 5
M3 - Article
PY - 2023
ST - Application of silver nanoparticles for improving motor recovery after spinal
T2 - Heliyon
TI - Application of silver nanoparticles for improving motor recovery after spinal
85159150682&doi=10.1016%2fj.heliyon.2023.e15689&partnerID=40&md5=fdc9b48a7a449d92f3
92d7e0ae59c9fe
VL - 9
ID - 5021
ER -
TY - JOUR
AB - Background: Alzheimer disease (AD) is the most common type of dementia which
is becoming a primary problem in the present society, but it lacks effective
treatment methods and means of AD. Tanshinone IIA (Tan IIA) has been reported to
have neuroprotective effects to restrain the Aβ25-35-mediated apoptosis. However,
few studies try to understand how Aβ1-42 affects hyperphosphorylation of tau and
how Tan IIA regulates this process at the molecular level. Methods: Fifty male
Sprague-Dawley rats were randomly divided into 5 groups and infused through the
lateral ventricle with Aβ1-42 except the control group. Then the rats were treated
with Tan IIA through intragastric administration for 4 weeks. After the ability of
learning and memory being measured, histomorphological examination and Western blot
were used to detect the possible mechanism in the AD-associated model rats.
Results: We observed that Aβ1-42 infusion could induce spatial learning and memory
deficits in rats. Simultaneously, Aβ1-42 also could reduce the neuron in cornu
ammonis 1 and dentate gyrus of hippocampus, as well as increase the levels of
cleaved caspase 3, hyperphosphorylated tau at the sites Ser396, Ser404, and Thr205
with enhancing staining of black granules in brain. We also found that Aβ1-42 could
increase the activity of extracellular signal-regulated protein kinase (ERK) and
glycogen synthase kinase-3β (GSK-3β). Meanwhile, these phenomena could be
ameliorated when Tan IIA was used. Conclusion: We concluded that Tan IIA might have
neuroprotective effect and improving learning and memory ability to be a viable
candidate in AD therapy with mechanisms involving the ERK and GSK-3β signal
pathway. © The Author(s) 2019.
AU - Lin, L.
AU - Jadoon, S. S.
AU - Liu, S. Z.
AU - Zhang, R. Y.
AU - Li, F.
AU - Zhang, M. Y.
AU - Ai-Hua, T.
AU - You, Q. Y.
AU - Wang, P.
DB - Scopus
DO - 10.1177/0891988719837373
IS - 3
KW - Alzheimer disease
extracellular signal-regulated protein kinase
glycogen synthase kinase-3β
hyperphosphorylated tau protein
spatial learning and memory deficits
tanshinones IIA
Abietanes
Animals
Glycogen Synthase Kinase 3 beta
Humans
Male
Memory Disorders
Rats
Spatial Learning
caspase 3
glycogen synthase kinase 3beta
tanshinone IIA
tau protein
tanshinone
amnesia
animal experiment
animal model
animal tissue
Article
brain slice
cell loss
cognitive defect
controlled study
drug mechanism
hippocampus
histology
male
MAPK signaling
memory consolidation
Morris water maze test
nerve degeneration
nerve fiber
neuroapoptosis
Nissl staining
nonhuman
phosphorylation
priority journal
protein expression
rat
silver staining
spatial learning
Western blotting
animal
disease model
drug effect
human
memory disorder
pharmacology
Sprague Dawley rat
M3 - Article
PY - 2019
SP - 152-163
ST - Tanshinone IIA Ameliorates Spatial Learning and Memory Deficits by Inhibiting
the Activity of ERK and GSK-3β
T2 - Journal of Geriatric Psychiatry and Neurology
TI - Tanshinone IIA Ameliorates Spatial Learning and Memory Deficits by Inhibiting
the Activity of ERK and GSK-3β
85063339362&doi=10.1177%2f0891988719837373&partnerID=40&md5=c00f834bd68c9e57818339f
ff502ad0c
VL - 32
ID - 5427
ER -
TY - JOUR
AB - The employment of nanoparticles has markedly increased in recent years for
different applications such as aerospace technology, electronics, biology, and
medicine. The exposure of nanoparticles to humans is inevitable nowadays.
Neutrophils act as the predominant phagocytic cells for first-line defense to be
recruited to an inflammatory site against xenobiotics. It is important to explore
how neutrophils interact with nanoparticles to elicit immune responses. Different
types of nanoparticles have been studied to reveal a potential interaction to
neutrophils in vitro and in vivo. These mainly include metallic nanoparticles,
polymeric nanoparticles, silica nanoparticles, lipid nanoparticles, and fullerenes.
A number of investigations have reported the toxicity of nanoparticles induced by
neutrophil activation. In this review we discuss data demonstrating recently
recognized aspects of inflammation induced by overwhelmed neutrophils after
nanoparticle treatment. Besides the dark side of the nanoparticles, some
therapeutic nanoparticles are developed and beneficial in treating neutrophil-
related diseases such as acute lung injury, vascular inflammation, and bacterial
infection. Some nanoparticles can recruit neutrophils around tumor sites for
immunotherapy. We also summarize how nanoparticles actively target neutrophils with
therapeutic aims. This review provides a broad introduction to nanoparticle
interplay with neutrophils and discusses in vitro and in vivo studies in which they
have been evaluated.
AN - WOS:000425869200004
AU - Lin, M. H.
AU - Lin, C. F.
AU - Yang, S. C.
AU - Hung, C. F.
AU - Fang, J. Y.
DA - JAN
DO - 10.1166/jbn.2018.2459
IS - 1
PY - 2018
SN - 1550-7033
1550-7041
SP - 66-85
ST - The Interplay Between Nanoparticles and Neutrophils
TI - The Interplay Between Nanoparticles and Neutrophils
VL - 14
ID - 6806
ER -
TY - JOUR
AB - The bacterial exopolysaccharide Curdlan has a unique collagen-like triple
helical structure and immune-modulation activities. Although there have been
several types of Curdlan gels reported for antibacterial or wound healing purposes,
none of them exhibit favorable mechanical properties for clinically applicable
wound healing materials. Herein, we present a two-step approach for preparing Ag-
embedded Curdlan hydrogels that are highly soft but are very stretchable compared
with common polysaccharide-based hydrogels. Ag ions were first reduced in a diluted
Curdlan solution to form AgNP-decorated triple helices. Then, the aqueous solution
consisting of Curdlan/Ag nanoparticles was mixed with a dimethyl sulfoxide solution
consisting of a high concentration of Curdlan. This mixing triggered the
conformation transformation of Curdlan random coils into triple helices, and then
the helices were further packed into semicrystalline nanofibrils of ∼20 nm in
diameter. Due to the presence of semicrystalline fibrils, this novel Curdlan
hydrogel exhibits a fracture strain of ∼350% and fracture stress of ∼0.2 MPa at a
water content of ∼97%. This nanofibril hydrogel supported the attachment,
spreading, and growth of fibroblasts and effectively inhibited the growth of Gram-
negative Escherichia coli and Gram-positive Staphylococcus aureus. Moreover, the
hydrogels downregulated NO production and proinflammatory gene expression levels in
lipopolysaccharide (LPS)-stimulated macrophages but did not change the anti-
inflammatory gene expression levels in IL-4-stimulated macrophages. In an animal
study, these hydrogels accelerated wound healing in a bacteria-infected mice skin
wound model. These results validate the further development of Curdlan/AgNPs
nanofibril hydrogels in clinical wound management. © 2021 American Chemical
Society.
AU - Lin, M.
AU - Long, H.
AU - Liang, M.
AU - Chu, B.
AU - Ren, Z.
AU - Zhou, P.
AU - Wu, C.
AU - Liu, Z.
AU - Wang, Y.
DB - Scopus
DO - 10.1021/acsami.1c06603
IS - 31
KW - antibacterial
Curdlan
hydrogels
nanofiber
wound healing
Animals
beta-Glucans
Carbohydrate Conformation
Escherichia coli
Hydrogels
Male
Metal Nanoparticles
Mice
Mice, Inbred BALB C
Nanofibers
NIH 3T3 Cells
Silver
Skin
Staphylococcal Skin Infections
Tensile Strength
Wound Healing
Cell culture
Dimethyl sulfoxide
Gene expression
Macrophages
Mammals
Tissue regeneration
antiinfective agent
beta glucan
curdlan
metal nanoparticle
silver
Anti-inflammatories
Exopolysaccharides
Gene expression levels
Immune modulation
Lipopolysaccharides
Semicrystallines
Triple helical structures
Two-step approach
animal
Bagg albino mouse
chemistry
conformation
drug effect
hydrogel
male
mouse
NIH 3T3 cell line
pathology
skin
staphylococcal skin infection
tensile strength
toxicity
Food additives
M3 - Article
PY - 2021
SP - 36747-36756
ST - Antifracture, Antibacterial, and Anti-inflammatory Hydrogels Consisting of
Silver-Embedded Curdlan Nanofibrils
TI - Antifracture, Antibacterial, and Anti-inflammatory Hydrogels Consisting of
Silver-Embedded Curdlan Nanofibrils
85112495843&doi=10.1021%2facsami.1c06603&partnerID=40&md5=b98a297528fa4338b350cf9b8
684025b
VL - 13
ID - 5219
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) are well known to interact with cells, leading to
different cell behaviors such as cell proliferation and differentiation capacity.
Biocompatibility and biological functions enhanced by nanomedicine are the most
concerning factors in clinical approaches. In the present research, AuNP solutions
were prepared at concentrations of 1.25, 2.5, 5 and 10 ppm for biocompatibility
investigations. Ultraviolet-visible spectroscopy was applied to identify the
presence of AuNPs under the various concentrations. Dynamic Light Scattering assay
was used for the characterization of the size of the AuNPs. The shape of the AuNPs
was observed through a Scanning Electron Microscope. Afterward, the mesenchymal
stem cells (MSCs) were treated with a differentiation concentration of AuNP
solutions in order to measure the biocompatibility of the nanoparticles. Our
results demonstrate that AuNPs at 1.25 and 2.5 ppm could significantly enhance MSC
proliferation, decrease reactive oxygen species (ROS) generation and attenuate
platelet/monocyte activation. Furthermore, the MSC morphology was observed in the
presence of filopodia and lamellipodia while being incubated with 1.25 and 2.5 ppm
AuNPs, indicating that the adhesion ability was enhanced by the nanoparticles. The
expression of matrix metalloproteinase (MMP-2/9) in MSCs was found to be more
highly expressed under 1.25 and 2.5 ppm AuNP treatment, relating to better cell
migrating ability. Additionally, the cell apoptosis of MSCs investigated with
Annexin-V/PI double staining assay and the Fluorescence Activated Cell Sorting
(FACS) method demonstrated the lower population of apoptotic cells in 1.25 and 2.5
ppm AuNP treatments, as compared to high concentrations of AuNPs. Additionally,
results from a Western blotting assay explored the possibility that the anti-
apoptotic proteins Cyclin-D1 and Bcl-2 were remarkably expressed. Meanwhile, real-
time PCR analysis demonstrated that the 1.25 and 2.5 ppm AuNP solutions induced a
lower expression of inflammatory cytokines (TNF-alpha, IL-1 beta, IFN-gamma, IL-6
and IL-8). According to the tests performed on an animal model, AuNP 1.25 and 2.5
ppm treatments exhibited the better biocompatibility performance, including anti-
inflammation and endothelialization. In brief, 1.25 and 2.5 ppm of AuNP solution
was verified to strengthen the biological functions of MSCs, and thus suggests that
AuNPs become the biocompatibility nanomedicine for regeneration research.
AN - WOS:000909645500001
AU - Lin, R. H.
AU - Lee, H. T.
AU - Yeh, C. A.
AU - Yang, Y. C.
AU - Shen, C. C.
AU - Chang, K. B.
AU - Liu, B. S.
AU - Hsieh, H. H.
AU - Wang, H. M. D.
AU - Hung, H. S.
C7 - 5
DA - JAN
DO - 10.3390/ijms24010005
IS - 1
PY - 2023
SN - 1422-0067
ST - Favorable Biological Performance Regarding the Interaction between Gold
Nanoparticles and Mesenchymal Stem Cells
TI - Favorable Biological Performance Regarding the Interaction between Gold
Nanoparticles and Mesenchymal Stem Cells
VL - 24
ID - 6268
ER -
TY - JOUR
AB - Objective. Currently, infections due to foreign-body reactions caused by
bacteria or implant materials at the wound site are one of the major reasons for
the failure of guided tissue regeneration (GTR) and guided bone regeneration (GBR)
in clinical applications. The purpose of this study was to develop regeneration
membranes with localized cobalt ion release to reduce infection and inflammation by
polycaprolactone (PCL)/cobalt-substituted hydroxyapatite (CoHA). Methods. The PCL
composite membrane containing 20 wt% CoHA powders was prepared by solvent casting.
The surface morphology, crystal structure, chemical composition and thermal
properties of PCL composite membranes were characterized. The biocompatibility,
osteogenic differentiation and antibacterial properties of composite membrane were
also investigated. Then, in biodegradability was assessed by immersing phosphate
buffer solution (PBS) for 6 months. Results. Physicochemical analyses revealed that
CoHA is evenly mixed in the membranes and assistance reduce the crystallinity of
PCL for getting more degradation amounts than PCL membrane. Osteoblast cells
culture on the membrane showed that the CoHA significantly increases cell
proliferation and found the calcium deposition production increased over 90%
compared with PCL after 7 days of culture. A good antibacterial effect was achieved
by the addition of CoHA powder. The results were confirmed by 2.4 times reduction
of proliferation of Escherichia coli (E. coli) seeded on the composite membrane
after 24 h. Immersing in PBS for 6 months indicated that PCL-CoHA composite
membrane has improved biodegradation and can continuously remove free radicals to
reduce the inflammatory response. Significance. The PCL-CoHA composite membrane
with suitable releasing of cobalt ion can be considered as a potential choice for
bone tissue regeneration. (C) 2019 The Academy of Dental Materials. Published by
Elsevier Inc. All rights reserved.
AN - WOS:000464117700010
AU - Lin, W. C.
AU - Yao, C. M.
AU - Huang, T. Y.
AU - Cheng, S. J.
AU - Tang, C. M.
DA - MAY
DO - 10.1016/j.dental.2019.02.023
IS - 5
PY - 2019
SN - 0109-5641
1879-0097
SP - 751-762
ST - Long-term in vitro degradation behavior and biocompatibility of
polycaprolactone/cobalt-substituted hydroxyapatite composite for bone tissue
engineering
T2 - DENTAL MATERIALS
TI - Long-term in vitro degradation behavior and biocompatibility of
polycaprolactone/cobalt-substituted hydroxyapatite composite for bone tissue
engineering
VL - 35
ID - 6194
ER -
TY - JOUR
AB - Diabetic mellitus is one of the leading causes of chronic wounds and remains
a challenging issue to be resolved. Herein, a hydrogel with conformal tissue
adhesivity, skin-like conductivity, robust mechanical characteristics, as well as
active antibacterial function is developed. In this hydrogel, silver nanoparticles
decorated polypyrrole nanotubes (AgPPy) and cobalt ions (Co2+) are introduced into
an in situ polymerized poly(acrylic acid) (PAA) and branched poly(ethylenimine)
(PEI) network (PPCA hydrogel). The PPCA hydrogel provides active antibacterial
function through synergic effects from protonated PEI and AgPPy nanotubes, with a
tissue-like mechanical property (approximate to 16.8 +/- 4.5 kPa) and skin-like
electrical conductivity (approximate to 0.048 S m(-1)). The tensile and shear
adhesive strength (approximate to 15.88 and approximate to 12.76 kPa, respectively)
of the PPCA hydrogel is about two- to threefold better than that of fibrin glue. In
vitro studies show the PPCA hydrogel is highly effective against both gram-positive
and gram-negative bacteria. In vivo results demonstrate that the PPCA hydrogel
promotes diabetic wounds with accelerated healing, with notable inflammatory
reduction and prominent angiogenesis regeneration. These results suggest the PPCA
hydrogel provide a promising approach to promote diabetic wound healing.
AN - WOS:000883317900001
AU - Lin, Z. C.
AU - Fan, D. H.
AU - Li, G. J.
AU - He, L. M.
AU - Qin, X. Y.
AU - Zhao, B.
AU - Wang, Q.
AU - Liang, W. L.
C6 - NOV 2022
DA - FEB
DO - 10.1002/mabi.202200349
IS - 2
PY - 2023
SN - 1616-5187
1616-5195
ST - Antibacterial, Adhesive, and Conductive Hydrogel for Diabetic Wound Healing
T2 - MACROMOLECULAR BIOSCIENCE
TI - Antibacterial, Adhesive, and Conductive Hydrogel for Diabetic Wound Healing
VL - 23
ID - 6515
ER -
TY - JOUR
AB - The concentration and distribution Of trace and major elements in cells are
of great interest in cell biology. PIXE can provide elemental concentrations in the
bulk of cells or organelles as other bulk techniques such as atomic absorption
spectrophotometry and nuclear activation analysis. Supplementary information,
perhaps more exciting, on the intracellular distributions of trace elements can be
provided using nuclear microscopy. Intracellular distributions of trace elements in
normal and malignant cells are presented. The toxicity of mercury and cadmium can
be prevented by supplementation of the essential trace element selenium. Some
results from an experimental animal model are discussed. The intercellular
distribution of major and trace elements in isolated blood cells, as revealed by
nuclear microscopy, provides useful clinical information. Examples are given
concerning inflammatory connective-tissue diseases and the chronic fatigue
syndrome.
AN - WOS:A1993KZ78200041
AU - Lindh, U.
DA - MAY
DO - 10.1016/0168-583X(93)95553-H
IS - 1-4
PY - 1993
SN - 0168-583X
1872-9584
SP - 261-267
ST - NUCLEAR MICROSCOPY IN TRACE-ELEMENT BIOLOGY - FROM CELLULAR STUDIES TO THE
CLINIC
T2 - NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS
WITH MATERIALS AND ATOMS
TI - NUCLEAR MICROSCOPY IN TRACE-ELEMENT BIOLOGY - FROM CELLULAR STUDIES TO THE
CLINIC
VL - 77
ID - 6657
ER -
TY - JOUR
AB - A 13-year-old Thoroughbred/Oldenburg gelding presented as a tertiary referral
for a 6-year history of immune-mediated keratitis (IMMK) after the development of
corneal and limbal nodules in the presence of medical therapy. A keratectomy
extending to the limbus was performed to remove the affected cornea and limbal
nodules. Two corneal-limbal samples were submitted for histopathology and initially
diagnosed as marked lympho-histiocytic stromal keratitis with epithelial
hyperplasia, keratinisation and keratinocyte apoptosis consistent with IMMK.
Additional testing with immunohistochemical staining for CD3, CD20, PAX5 and IBA1
revealed large, atypical B-lymphocytes on a background of histiocytes and mature T-
lymphocytes, with concern for T-cell-rich large B-cell lymphoma. PCR antigen
receptor rearrangement clonality testing was also performed and was diagnostic for
large B-cell lymphoma. Polymerase chain reaction (PCR) for equine herpesvirus 5
(EHV-5) was positive. Following keratectomy, a short course of neomycin/polymyxin
B/dexamethasone ophthalmic ointment was prescribed; no other treatment nor adjunct
therapy was performed. Eighteen months post-operation, the case remains visual and
comfortable on no medications. © 2022 EVJ Ltd.
AU - Lisankis, A. P.
AU - Beavis, B. B.
AU - Weigt, A. K.
AU - Nunnery, C.
DB - Scopus
DO - 10.1111/eve.13725
IS - 5
KW - B-cell lymphoma
equine herpesvirus 5
horse
immune-mediated keratitis
neoplastic transformation
atropine
CD20 antigen
CD3 antigen
detomidine
dexamethasone plus neomycin plus polymyxin B
diclofenac
flunixin meglumine
lidocaine
lymphocyte antigen receptor
mepivacaine
ofloxacin
proxymetacaine
sulfadiazine
sulfadiazine silver
tacrolimus
transcription factor PAX5
trimethoprim
xylazine
animal tissue
apoptosis
Article
B cell lymphoma
B lymphocyte
blepharospasm
conjunctival hyperemia
cornea limbus
edema
Equid herpesvirus 1
equid herpesvirus 5
gelding
histiocyte
histopathology
immune mediated keratitis
keratectomy
keratinocyte
keratitis
nonhuman
Oldenburg Burnout Inventory
remission
T lymphocyte
tertiary care center
Thoroughbred horse
ulcer
M3 - Article
PY - 2023
SP - e346-e351
ST - A case of malignant transformation of equine immune-mediated keratitis to B-
cell lymphoma
T2 - Equine Veterinary Education
TI - A case of malignant transformation of equine immune-mediated keratitis to B-
cell lymphoma
85141703716&doi=10.1111%2feve.13725&partnerID=40&md5=b3b0615acafbd86492f396e51ec9aa
fa
VL - 35
ID - 4965
ER -
TY - JOUR
AB - Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are
severe adverse drug reactions, characterized by a low incidence but high mortality,
initially described as separate entities, but today considered variants of the same
pathologic process and differing only for severity. The majority of cases appear to
be related to idiosyncratic drug reactions. The drugs most commonly involved are:
antibiotics such as sulfonamides, β-lactam, tetracyclines and quinolones;
anticonvulsants such as phenytoin, phenobarbital and carbamazapine; antiretroviral
drugs; nonsteroidal anti-inflammatory drugs, allopurinol. There is common agreement
to consider TEN as the manifestation of a disregulated immune reaction against
epithelial cells. During the first stages of TEN, apoptosis mediates keratinocyte
death and the pivotal role of Fas-FasL pathway activation during TEN is undoubted.
T cell cytotoxicity, demonstrated during TEN, has been shown to be mediated by the
perforin-granzyme pathway. It seems, also, clear that a peculiar cytokine pattern
plays an important role in TEN pathogenesis. The cutaneous findings result in an
acute macular erythematous rash with bullae. These lesions rapidly exhibit
Nikolsky's sign and a separation of large sheets of epidermis from the dermis and a
subsequent localised shedding develops rapidly, which can become very extensive.
When feasible, admission in burn or intensive care unit, positioning the patients
in air-fluidised beds, is universally considered crucial in TEN treatment. The
prompt withdrawal of the suspected drug, fluid and electrolyte replacement and
topical wound care are the first line of therapy. The use of corticosteroids has
been abandoned and the role of immunosuppressants, despite some success, is not
well defined and is not considered as a standard. A trial comparing thalidomide
versus placebo in TEN patients was suspended because mortality rate increased in
the treated group. Infliximab, a chimeric monoclonal antibody to TNF-α, has been
administered to a patient, in single infusion, with a favourable outcome.
Plasmapheresis is reported to lead to some success in TEN treatment, with
improvement of clinical conditions and high percentage of survival. Different
authors reported good results in terms of decreasing mortality and morbidity or
improving clinical conditions of the use of human intravenous immunoglobulins
(IVIGs). Regardless, the true utility of this treatment remains controversial. In
2005, the authors (ML and RC), dealing with a number of severe TEN cases, proposed
a new protocol based on the combination of these last two techniques reporting
their preliminary results in the treatment of severe TEN patients. © 2009 Elsevier
Ltd and ISBI.
AU - Lissia, M.
AU - Mulas, P.
AU - Bulla, A.
AU - Rubino, C.
DB - Scopus
DO - 10.1016/j.burns.2009.06.213
IS - 2
KW - Drug reactions
IVIG
Lyell's disease
Plasmapheresis
SCORTEN
Stevens-Johnson's syndrome
Epidermal Necrolysis, Toxic
Humans
Immunoglobulins, Intravenous
Skin
biobrane
corticosteroid
cyclophosphamide
cyclosporin
immunoglobulin
infliximab
methylprednisolone
sulfadiazine silver
sulfonamide
thalidomide
tumor necrosis factor alpha antibody
antibiotic therapy
burn
clinical trial
Crohn disease
drug hypersensitivity
graft rejection
graft versus host reaction
human
immunopathology
plasmapheresis
review
skin injury
Stevens Johnson syndrome
total parenteral nutrition
toxic epidermal necrolysis
wound care
wound dressing
wound infection
M3 - Review
PY - 2010
SP - 152-163
ST - Toxic epidermal necrolysis (Lyell's disease)
T2 - Burns
TI - Toxic epidermal necrolysis (Lyell's disease)
76349090218&doi=10.1016%2fj.burns.2009.06.213&partnerID=40&md5=5f1da0ed1d6d21ed8c41
2f5bab150434
VL - 36
ID - 5712
ER -
TY - JOUR
AB - Group 3 innate lymphoid cells (ILC3) promote lymphoid organogenesis and
potentiate immune responses against bacterial infection. However, how ILC3 cells
are developed and maintained is still unclear. Here, we show that carboxypeptidase
CCP2 is highly expressed in common helper-like innate lymphoid progenitors, the
progenitor of innate lymphoid cells, and CCP2 deficiency increases ILC3 numbers.
Interleukin-7 receptor subunit alpha (IL-7Rα) is identified as a substrate of CCP2
for deglutamylation, and IL-7Rα polyglutamylation is catalyzed by polyglutamylases
TTLL4 and TTLL13 in common helper-like innate lymphoid progenitors. IL-7Rα
polyglutamylation triggers STAT5 activation to initiate transcription factor Sall3
expression in common helper-like innate lymphoid progenitors, which drives ILC3
cell differentiation. Moreover, Ttll4 -/- or Ttll13 -/- mice have reduced IL-7Rα
polyglutamylation and Sall3 expression in common helper-like innate lymphoid
progenitors. Importantly, mice with IL-7Rα E446A mutation have reduced Sall3
expression and ILC3 population. Thus, polyglutamylation and deglutamylation of IL-
7Rα tightly controls the development and effector functions of ILC3s. © 2017 The
Author(s).
AU - Liu, B.
AU - Ye, B.
AU - Zhu, X.
AU - Huang, G.
AU - Yang, L.
AU - Zhu, P.
AU - Du, Y.
AU - Wu, J.
AU - Meng, S.
AU - Tian, Y.
AU - Fan, Z.
C7 - 231
DB - Scopus
DO - 10.1038/s41467-017-00235-x
IS - 1
KW - Animals
Glutamic Acid
Granzymes
Homeodomain Proteins
Immunity, Innate
Lymphocytes
Mice
Peptide Synthases
Receptors, Interleukin-7
Transcription Factors
Mus
carboxypeptidase
carboxypeptidase CCP2
interleukin 22
interleukin 23
interleukin 7
interleukin 7 receptor alpha
messenger RNA
short hairpin RNA
STAT3 protein
STAT5 protein
transcription factor sall3
unclassified drug
glutamic acid
granzyme
Gzmc protein, mouse
homeodomain protein
interleukin 7 receptor
interleukin-7 receptor, alpha chain
peptide synthase
Sall3 protein, mouse
bacterial disease
enzyme activity
gene expression
immune response
infectivity
mutation
rodent
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
bacterial load
catalysis
cell count
cell lysate
cell maturation
chromatin immunoprecipitation
comparative study
controlled study
feeder cell
female
flow cytometry
gel mobility shift assay
group 3 innate lymphoid cell
immunoblotting
immunofluorescence test
in vitro study
inflammatory cell
luciferase assay
lymphoid cell
mouse
nonhuman
promoter region
protein expression
sequence analysis
silver staining
animal
cytology
genetics
immunology
innate immunity
lymphocyte
metabolism
physiology
M3 - Article
PY - 2017
ST - IL-7Rα glutamylation and activation of transcription factor Sall3 promote
group 3 ILC development
T2 - Nature Communications
TI - IL-7Rα glutamylation and activation of transcription factor Sall3 promote
group 3 ILC development
85027219970&doi=10.1038%2fs41467-017-00235-
x&partnerID=40&md5=4ff2cb0e6595e942e9ae4a59628afd08
VL - 8
ID - 5518
ER -
TY - JOUR
AB - BackgroundPD-1/PD-L1 immune checkpoint inhibitors are currently the most
commonly utilized agents in clinical practice, which elicit an immunostimulatory
response to combat malignancies. However, all these inhibitors are currently
administered via injection using antibody-based therapies, while there is a growing
need for oral alternatives. MethodsThis study has developed and synthesized
exosome-wrapped gold-peptide nanocomplexes with low immunogenicity, which can
target PD-L1 and activate antitumor immunity in vivo through oral absorption. The
(Super)PDL1(exo) was characterized by transmission electron microscopy (TEM),
dynamic light scattering (DLS), Fourier transform infrared (FTIR), X-ray
photoelectron spectroscopy (XPS), and gel silver staining. The transmembrane
ability of (Super)PDL1(exo) was evaluated by flow cytometry and immunofluorescence.
Cell viability was determined using the Cell Counting Kit-8 (CCK-8) assay. ELISA
experiments were conducted to detect serum and tissue inflammatory factors, as well
as serum biochemical indicators. Tissue sections were stained with H & E for the
evaluation of the safety of (Super)PDL1(exo). An MC38 colon cancer model was
established in immunocompetent C56BL/6 mice to evaluate the effects of
(Super)PDL1(exo) on tumor growth in vivo. Immunohistochemistry (IHC) staining was
performed to detect cytotoxicity factors such as perforin and granzymes.
ResultsFirst, (Super)PDL1 was successfully synthesized, and milk exosome membranes
were encapsulated through ultrasound, repeated freeze-thaw cycles, and extrusion,
resulting in the synthesis of (Super)PDL1(exo). Multiple characterization results
confirmed the successful synthesis of (Super)PDL1(exo) nanoparticles. Furthermore,
our data demonstrated that (Super)PDL1(exo) exhibited excellent colloidal stability
and superior cell transmembrane ability. In vitro and in vivo experiments revealed
that (Super)PDL1(exo) did not cause damage to multiple systemic organs,
demonstrating its good biocompatibility. Finally, in the MC38 colon cancer mouse
model, it was discovered that (Super)PDL1(exo) could inhibit the progression of
colon cancer, and this tumor-suppressive effect was mediated through the activation
of tumor-specific cytotoxic T lymphocyte (CTL)-related immune responses.
ConclusionThis study has successfully designed and synthesized an oral
nanotherapeutic, (Super)PDL1(exo), which demonstrates small particle size,
excellent colloidal stability, transmembrane ability in tumor cells, and
biocompatibility. In vivo experiments have shown that it effectively activates T-
cell immunity and exerts antitumor effects.
AN - WOS:001039771000001
AU - Liu, D.
AU - Wang, J. M.
AU - You, W. M.
AU - Ma, F.
AU - Sun, Q.
AU - She, J. J.
AU - He, W. X.
AU - Yang, G.
C7 - 1228581
DA - JUL 17
DO - 10.3389/fimmu.2023.1228581
PY - 2023
SN - 1664-3224
ST - A d-peptide-based oral nanotherapeutic modulates the PD-1/PD-L1 interaction
for tumor immunotherapy
TI - A d-peptide-based oral nanotherapeutic modulates the PD-1/PD-L1 interaction
VL - 14
ID - 6606
ER -
TY - JOUR
AB - Background: PD-1/PD-L1 immune checkpoint inhibitors are currently the most
commonly utilized agents in clinical practice, which elicit an immunostimulatory
response to combat malignancies. However, all these inhibitors are currently
administered via injection using antibody-based therapies, while there is a growing
need for oral alternatives. Methods: This study has developed and synthesized
exosome-wrapped gold–peptide nanocomplexes with low immunogenicity, which can
target PD-L1 and activate antitumor immunity in vivo through oral absorption. The
SuperPDL1exo was characterized by transmission electron microscopy (TEM), dynamic
light scattering (DLS), Fourier transform infrared (FTIR), X-ray photoelectron
spectroscopy (XPS), and gel silver staining. The transmembrane ability of
SuperPDL1exo was evaluated by flow cytometry and immunofluorescence. Cell viability
was determined using the Cell Counting Kit-8 (CCK-8) assay. ELISA experiments were
conducted to detect serum and tissue inflammatory factors, as well as serum
biochemical indicators. Tissue sections were stained with H&E for the evaluation of
the safety of SuperPDL1exo. An MC38 colon cancer model was established in
immunocompetent C56BL/6 mice to evaluate the effects of SuperPDL1exo on tumor
growth in vivo. Immunohistochemistry (IHC) staining was performed to detect
cytotoxicity factors such as perforin and granzymes. Results: First, SuperPDL1 was
successfully synthesized, and milk exosome membranes were encapsulated through
ultrasound, repeated freeze–thaw cycles, and extrusion, resulting in the synthesis
of SuperPDL1exo. Multiple characterization results confirmed the successful
synthesis of SuperPDL1exo nanoparticles. Furthermore, our data demonstrated that
SuperPDL1exo exhibited excellent colloidal stability and superior cell
transmembrane ability. In vitro and in vivo experiments revealed that SuperPDL1exo
did not cause damage to multiple systemic organs, demonstrating its good
biocompatibility. Finally, in the MC38 colon cancer mouse model, it was discovered
that SuperPDL1exo could inhibit the progression of colon cancer, and this tumor-
suppressive effect was mediated through the activation of tumor-specific cytotoxic
T lymphocyte (CTL)-related immune responses. Conclusion: This study has
successfully designed and synthesized an oral nanotherapeutic, SuperPDL1exo, which
demonstrates small particle size, excellent colloidal stability, transmembrane
ability in tumor cells, and biocompatibility. In vivo experiments have shown that
it effectively activates T-cell immunity and exerts antitumor effects. Copyright ©
2023 Liu, Wang, You, Ma, Sun, She, He and Yang.
AU - Liu, D.
AU - Wang, J.
AU - You, W.
AU - Ma, F.
AU - Sun, Q.
AU - She, J.
AU - He, W.
AU - Yang, G.
C7 - 1228581
DB - Scopus
DO - 10.3389/fimmu.2023.1228581
KW - anti-tumor
immunotherapy
milk exosome
peptide
supramolecular nanospheres
Animals
B7-H1 Antigen
Cell Line, Tumor
Colonic Neoplasms
Immunotherapy
Mice
Peptides
Programmed Cell Death 1 Receptor
d peptide
granzyme
nanoparticle
nanosphere
perforin
programmed death 1 ligand 1
unclassified drug
absorption
animal experiment
animal model
animal tissue
apoptosis
Article
biocompatibility
cancer growth
cancer immunotherapy
cell infiltration
cell viability
cellular immunity
clinical practice
colon cancer
colorectal cancer
cytotoxic T lymphocyte
cytotoxicity
encapsulation
enzyme activity
excitation
exosome
flow cytometry
Fourier transform
immune response
immunofluorescence
immunogenicity
immunostimulation
in vivo study
milk
mouse
nanomedicine
nonhuman
oral absorption
particle size
silver staining
synthesis
T lymphocyte
tissue section
tumor growth
tumor immunity
tumor volume
ultrasound
zeta potential
animal
colon tumor
metabolism
procedures
tumor cell line
M3 - Article
PY - 2023
ST - A d-peptide-based oral nanotherapeutic modulates the PD-1/PD-L1 interaction
TI - A d-peptide-based oral nanotherapeutic modulates the PD-1/PD-L1 interaction
85166045120&doi=10.3389%2ffimmu.2023.1228581&partnerID=40&md5=8e77e417aac5649493e5d
f8c00b17d04
VL - 14
ID - 5025
ER -
TY - JOUR
AB - Manufactured zinc oxide nanoparticles (Nano-ZnO) are being used increasingly
in many fields owing to their excellent physicochemical properties. Consequently,
biosecurity has become a growing concern for human health and the environment. In
the present study. Nano-ZnO neurotoxidty was investigated in vivo and in vitro. in
vivo results showed that Nano-ZnO particles delivered through intranasal
instillation were translocated to the brain, specifically deposited in the
olfactory bulb, hippocampus, striatum, and cerebral cortex, and caused
ultrastructural changes, oxidative damage, inflammatory responses, and
histopathological damages there, which may be important for inducing Nano-ZnO
neurotoxidty. Further in vitro studies on PC12 cell line illustrated that exposure
to Nano-ZnO for 6 h affected cell morphology, decreased cell viability, increased
lactate dehydrogenase and oxidative stress activity levels, impaired mitochondrial
function, and disturbed the cell cycle. In addition, Nano-ZnO could destroy
neuronal structure by affecting cytoskeleton proteins (tubulin-alpha, tubulin-beta
and NF-H), resulting in the interruption of connection between nerve cells, which
lead to nervous system function damage. Meanwhile, Nano-ZnO could induce neuronal
repair and regeneration disorders by affecting the growth-related protein GAP43 and
delayed neurotoxidty by affecting the calcitun/caldum-regulated kinase
(CAMK2A/CAMK2B protein) signaling pathway. (C) 2019 Elsevier B.V. All rights
reserved.
AN - WOS:000508129700061
AU - Liu, H. L.
AU - Yang, H. L.
AU - Fang, Y. J.
AU - Li, K.
AU - Tian, L.
AU - Li, X. H.
AU - Zhang, W.
AU - Tan, Y. Z.
AU - Lai, W. Q.
AU - Bian, L. P.
AU - Lin, B. C.
AU - Xi, Z. G.
C7 - 135809
DA - FEB 25
DO - 10.1016/j.scitotenv.2019.135809
PY - 2020
SN - 0048-9697
1879-1026
ST - Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional
brain regions and dopaminergic neurons
T2 - SCIENCE OF THE TOTAL ENVIRONMENT
TI - Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional
brain regions and dopaminergic neurons
VL - 705
ID - 6751
ER -
TY - JOUR
AB - Zinc oxide, titanium dioxide and silver nanoparticles are used as
sterilization materials to enhance the performance of disinfectants. Here, the
toxicological effects on the liver, spleen, thymus gland, immune function and
inflammatory responses in rats induced by these nanoparticles were investigated
after intratracheal instillation in male Wistar rats. Moreover, the relationships
between the particle size, particle crystalline structure, chemical composition,
chemical stability and toxicological effects of these typical nanoparticles in rats
were explored. Exposure to nanoparticles increased the oxidative stress level in
peripheral blood and the homogenates of the liver, spleen and thymus as well as
disorders in regulating the cytokine network and blood cell count in the peripheral
blood. Furthermore, the histopathological study revealed that pulmonary exposure to
nanoparticles produced persistent, progressive liver inflammatory responses and
cell necrosis, while no observable damage was found in the kidney, thymus gland or
spleen tissue from the experimental groups. Our results demonstrate that oxidative
stress might be important for inducing the toxic effects of these nanoparticles,
and three nanoparticles can influence the immune function of rats. A comparative
analysis of the toxic effects of nanomaterials demonstrated significant
differences. Nano-ZnO induced the most significant toxicity, whereas nano-TiO2
induced the least. Particle composition and chemical stability probably played a
primary role in the toxicological effects of different nanoparticles. © 2015 The
AU - Liu, H. L.
AU - Yang, H. L.
AU - Lin, B. C.
AU - Zhang, W.
AU - Tian, L.
AU - Zhang, H. S.
AU - Xi, Z. G.
DB - Scopus
DO - 10.1039/c4tx00154k
IS - 2
KW - Rattus
Rattus norvegicus
silver nanoparticle
titanium dioxide
zinc oxide
animal experiment
Article
blood cell count
cell death
controlled study
crystal structure
double antibody sandwich enzyme linked immunosorbent assay
erythrocyte count
histopathology
immune response
inflammation
instrument sterilization
kidney
leukocyte count
liver homogenate
male
nonhuman
oxidative stress
particle size
priority journal
rat
thymus
toxicity testing
Wistar rat
M3 - Article
PY - 2015
SP - 486-493
ST - Toxic effect comparison of three typical sterilization nanoparticles on
oxidative stress and immune inflammation response in rats
T2 - Toxicology Research
TI - Toxic effect comparison of three typical sterilization nanoparticles on
oxidative stress and immune inflammation response in rats
84923870106&doi=10.1039%2fc4tx00154k&partnerID=40&md5=4e1c4f1e8c5fd2cfeaa5666b970c3
b08
VL - 4
ID - 5559
ER -
TY - JOUR
AB - Silver, zinc oxide, and titanium dioxide nanoparticles are used as
sterilisation materials to enhance the performance of disinfectants. We
investigated the respiratory tract immune toxicity (" immunotoxicity" ) of these
nanoparticles in vivo and in vitro, and we explored the relationships between
particle size, particle shape, chemical composition, chemical stability and the
toxicological effects of these typical nanoparticles in rats. In vivo, the rats
were exposed to nanoparticles by intratracheal instillation. Exposure to
nanoparticles caused an increase in oxidative injury to the lungs and disorders in
regulating the cytokine network, which were detected in the bronchoalveolar lavage
fluid, suggesting that oxidative stress might be important for inducing the
respiratory immunotoxicity of nanoparticles. In vitro, the phagocytic function of
alveolar macrophages (AMs) was dose-dependently reduced by nanoparticles, and ZnO
nanoparticles induced greater cytotoxicity than the silver and titanium-dioxide
nanoparticles, which were coincident with the results of multiple measurements,
such as a cell viability assay by WST-8 and LDH measurements. Comparative analyses
demonstrated that particle composition and chemical stability most likely had a
primary role in the biological effects of different nanoparticles. © 2013 Elsevier
B.V.
AU - Liu, H.
AU - Yang, D.
AU - Yang, H.
AU - Zhang, H.
AU - Zhang, W.
AU - Fang, Y.
AU - Lin, Z.
AU - Tian, L.
AU - Lin, B.
AU - Yan, J.
AU - Xi, Z.
DB - Scopus
DO - 10.1016/j.jhazmat.2013.01.046
IS - 1
KW - Comparative study
Respiratory tract immune toxicity
Titanium dioxide nanoparticles
Animals
Cell Survival
Cytokines
Glutathione
Macrophages, Alveolar
Malondialdehyde
Metal Nanoparticles
Nitric Oxide
Phagocytosis
Rats
Rats, Wistar
Respiratory System
Silver
Sterilization
Tetrazolium Salts
Titanium
Zinc Oxide
Nanoparticles
Oxides
Titanium dioxide
Toxicity
Zinc oxide
disinfectant agent
interleukin 1
interleukin 6
nanoparticle
silver nanoparticle
tetrazolium
titanium dioxide
unclassified drug
zinc oxide
Comparative studies
Immune toxicities
comparative study
disinfection
immune response
injury
nanotechnology
particle size
pollution exposure
respiratory disease
rodent
silver
sterilization
titanium
toxicity
toxicology
zinc
animal cell
animal experiment
animal model
article
cell viability
controlled study
cytotoxicity
exposure
immunotoxicity
in vitro study
in vivo study
instrument sterilization
lung lavage
measurement
nonhuman
oxidative stress
phagocyte
rat
respiratory tract disease
respiratory tract immune toxicity
M3 - Article
PY - 2013
SP - 478-486
ST - Comparative study of respiratory tract immune toxicity induced by three
sterilisation nanoparticles: Silver, Zinc oxide and titanium dioxide
TI - Comparative study of respiratory tract immune toxicity induced by three
sterilisation nanoparticles: Silver, Zinc oxide and titanium dioxide
84873725133&doi=10.1016%2fj.jhazmat.2013.01.046&partnerID=40&md5=84fc7599aca3511691
cfbb8a26f5b895
VL - 248-249
ID - 5691
ER -
TY - JOUR
AB - Purpose: Radiation recall dermatitis (RRD) is a rare complication that occurs
after completion of radiation therapy (RT) and initiation of a precipitating agent,
most commonly chemotherapeutic medications. Various theories attempt to explain the
mechanism, including activation of the body's inflammatory pathways through
nonimmune activation. Likewise, radiation-induced organizing pneumonia (RIOP) is an
infrequent but potentially life-threatening complication of RT that, while not
fully understood, is suspected to be partly an autoimmune reaction. Patient: We
present the case of a 71-year-old female with a history of type 2 diabetes
mellitus, hypothyroidism, interstitial cystitis, and osteoarthritis who presented
with clinical stage T1N0M0 ER+/PR-/HER2- invasive ductal carcinoma of the lower
outer quadrant of the left breast, for which she underwent left segmental
mastectomy and sentinel lymph node biopsy followed by completion axillary lymph
node dissection. Her final pathologic stage was T1N1M0. Result: The patient
developed RRD and later RIOP following receipt of radiation and chemotherapy, which
resolved with steroid administration. Conclusions: The rarity of both RRD and RIOP
occurring in a patient, as in our case, suggests a shared pathophysiology behind
these two complications. As both reactions involve some degree of inflammation and
respond to corticosteroids, it seems likely that the etiologies of RRD and RIOP lie
within the inflammatory pathway. However, further investigation should evaluate the
frequency, duration, and triggering of concomitant RRD and RIOP. © 2020 S. Karger
AG. All rights reserved.
AU - Liu, I. C.
AU - Giap, F.
AU - Mailhot-Vega, R. B.
AU - Bradley, J. A.
AU - Mendenhall, N. P.
AU - Okunieff, P.
AU - Lu, L.
AU - Jantz, M. A.
AU - Daily, K.
AU - Spiguel, L.
AU - Lockney, N. A.
DB - Scopus
DO - 10.1159/000508493
IS - 2
KW - Dermatitis
Radiation recall
Radiation toxicity
Radiation-induced organizing pneumonia
azithromycin
benzonatate
cefalexin
cotrimoxazole
cyclophosphamide
docetaxel
letrozole
miaderm
paracetamol
prednisone
radioprotective agent
sulfadiazine silver
adjuvant chemotherapy
aged
Article
breast cancer-related lymphedema
breast carcinoma
breast discomfort
breast disease
breast radiotherapy
breast tenderness
bronchoscopy
burning sensation
cancer staging
case report
clinical article
computed tomographic angiography
coughing
drug dose increase
drug dose reduction
drug withdrawal
dyspnea
eosinophilia
erythema
fatigue
female
fever
follow up
heat
human
human tissue
hyperglycemia
hypothyroidism
insomnia
interstitial cystitis
intraductal carcinoma
leukocyte differential count
lung biopsy
lung lavage
lymph node dissection
lymph vessel metastasis
lymphocytopenia
monocytosis
multiple cycle treatment
organizing pneumonia
osteoarthritis
oxygen supply
partial mastectomy
physiotherapy
priority journal
radiation dose
radiation pneumonia
sentinel lymph node biopsy
thorax radiography
M3 - Article
PY - 2020
SP - 875-882
ST - Concomitant Radiation Recall Dermatitis and Organizing Pneumonia following
Breast Radiotherapy: A Case Report
T2 - Case Reports in Oncology
TI - Concomitant Radiation Recall Dermatitis and Organizing Pneumonia following
Breast Radiotherapy: A Case Report
85089155124&doi=10.1159%2f000508493&partnerID=40&md5=689212058e401e9611d757c266bce1
f1
VL - 13
ID - 5381
ER -
TY - JOUR
AB - Due to the widespread applications of zinc oxide nanoparticles (ZnO NPs), the
potential exposure of workers, consumers, and scientists to these particles has
increased. This potential for exposure has attracted extensive attention in the
science community. Many studies have examined the toxicological profile of ZnO NPs
in the immune system, digestive system, however, information regarding the toxicity
of ZnO NPs in the nervous system is scarce. In this study, we detected the
cytotoxicity of two types of ZnO NPs of various sizes - ZnOa NPs and ZnOb NPs - and
we characterized the shedding ability of zinc ions within culture medium and the
cytoplasm. We found that reactive oxygen species played a crucial role in ZnO NP-
induced cytotoxicity, likely because zinc ions were leached from ZnO NPs. Apoptosis
and cytoskeleton changes were also toxic responses induced by the ZnO NPs, and ZnOb
NPs induced more significant toxic responses than ZnOa NPs in SHSY5Y cells. In
conclusion, ZnO NPs induced toxic responses in SHSY5Y cells in a size-dependent
manner, which can probably be attributed to their ion-shedding ability.
AN - WOS:000414171400001
AU - Liu, J.
AU - Kang, Y. Y.
AU - Yin, S. H.
AU - Song, B.
AU - Wei, L. M.
AU - Chen, L. J.
AU - Shao, L. Q.
DO - 10.2147/IJN.S149070
PY - 2017
SN - 1178-2013
SP - 8085-8099
ST - Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y
cells in a size-dependent manner
TI - Zinc oxide nanoparticles induce toxic responses in human neuroblastoma SHSY5Y
cells in a size-dependent manner
VL - 12
ID - 6335
ER -
TY - JOUR
AB - The use of contact lenses for the early treatment of bacterial or fungal
keratitis has become a new research focus. Two main I requirements of the
therapeutic contact lenses are antimicrobial ability and visible light
transmittance. Silver nanoparticles (AgNPs), as a nonspecific antimicrobial
component, have been loaded onto contact lenses for the treatment of bacterial and
fungal keratitis. Recently, it was reported that, via a simple immersion method,
AgNPs can be synthesized and fixed onto the surface of polydopamine (PDA)-coated
materials. However, in this study, we found that the above traditional method has
the disadvantages of poor AgNP loading and low visible light transmittance, which
could be induced by a limited amount of phenolic hydroxyl groups on and second
oxidation of the PDA coating, respectively. To overcome these disadvantages, in
this paper, we provided a facile and novel method to robustly bind multilayer-AgNPs
on contact lens surfaces by using dopamine as a reducing agent and bioglue. In
comparing with the monolayer-AgNPloaded contact lenses fabricated by the
traditional method, the multilayer-AgNP-loaded contact lenses had excellent
antimicrobial ability and better visible light transmittance. Moreover, the
multilayer-AgNP-loaded contact lens had low cytotoxicity to human corneal
epithelial cells and anti-inflammation properties. Furthermore, the shortcoming of
decreasing visible light transmittance induced by excess adherence of AgNPs on the
multilayer-AgNP-loaded contact lens was alleviated by decreasing the size of AgNPs
through altering the concentration of dopamine and AgNO3. Contact lenses loaded
with small AgNPs (Ag@PDA-2.5, diameter approximate to 25-50 nm) had approximately
the same Ag+ release and antimicrobial abilities, but significantly better visible
light transmittance and anti-inflammatory properties than the contact lenses loaded
with large AgNPs (Ag@PDA-2.5, diameter approximate to 50-75 nm). After that, in
vivo testing indicated the promising therapeutic strategy of multilayer-AgNP-loaded
contact lenses (Ag@ PDA-2.5) for early bacterial keratitis and fungal keratitis. In
addition, PDA coating could provide reactive sites to immobilize other biomolecules
or drugs on this multilayer-AgNP-loaded contact lens for further combination
therapies in treating bacterial or fungal keratitis. Finally, the stability of the
visible light transmittance of the multilayer-AgNP-loaded contact lens was
detected. The visible light transmittance of Ag@PDA-2.5 was weakened after being
cultured with an extremely high concentration of bacteria, while it was stable in
the moderate work environment. Though PDA coating had been wildly used to modify
implantation devices, however, few studies about PDA coating modified contact
lenses have been reported so far. Therefore, this research also provides an
important basis for using PDA coating to modify a therapeutic contact lens.
AN - WOS:000432476200010
AU - Liu, X. Q.
AU - Chen, J.
AU - Qu, C.
AU - Bo, G.
AU - Jiang, L.
AU - Zhao, H.
AU - Zhang, J.
AU - Lin, Y.
AU - Hua, Y.
AU - Yang, P.
AU - Huang, N.
AU - Yang, Z. L.
DA - MAY
DO - 10.1021/acsbiomaterials.7b00977
IS - 5
PY - 2018
SN - 2373-9878
SP - 1568-1579
ST - A Mussel-Inspired Facile Method to Prepare Multilayer-AgNP-Loaded Contact
Lens for Early Treatment of Bacterial and Fungal Keratitis
TI - A Mussel-Inspired Facile Method to Prepare Multilayer-AgNP-Loaded Contact
VL - 4
ID - 6565
ER -
TY - JOUR
AB - The treatment of diabetic wounds remains a global challenge. Compared with
traditional wound dressings, there are higher requirements of antibacterial, anti-
inflammatory and pro-angiogenic effects in diabetic wound dressings. Furthermore,
it is desirable for dressings to self-adapt to wounds with different morphologies
without extra processes and stably (suitable adhesive and self-healing abilities)
provide a conducive environment for wound healing. Herein, we construct an
injectable and self-healing hydrogel through the combination of chitosan (CS) and
metal ions to efficiently improve infected and diabetic wound healing. Benefiting
from the amino and hydroxy groups, the CS molecular chains are cross-linked with
silver ions (Ag+) and copper ions (Cu2+) to promote the formation of the CS-Ag-Cu
hydrogel, which releases Ag+ (an antibacterial agent) and Cu2+ (an angiogenic
agent) over a prolonged period. Moreover, the hydrogel possesses appropriate
adhesive ability, good water absorption ability, antibacterial capability and
biocompatibility according to in vitro investigations. In vivo experimental results
further prove that the CS-Ag-Cu hydrogel can dramatically accelerate tissue repair
in a Staphylococcus aureus (S. aureus)-infected skin incision model in normal rats
and diabetic wounds.
AN - WOS:000798233400001
AU - Liu, X. X.
AU - Zhou, S. J.
AU - Cai, B. Y.
AU - Wang, Y. A.
AU - Deng, D.
AU - Wang, X. L.
C6 - MAY 2022
DA - JUN 28
DO - 10.1039/d2bm00224h
IS - 13
PY - 2022
SN - 2047-4830
2047-4849
SP - 3480-3492
ST - An injectable and self-healing hydrogel with antibacterial and angiogenic
properties for diabetic wound healing
TI - An injectable and self-healing hydrogel with antibacterial and angiogenic
VL - 10
ID - 6581
ER -
TY - JOUR
AB - The use of contact lenses for the early treatment of bacterial or fungal
keratitis has become a new research focus. Two main requirements of the therapeutic
contact lenses are antimicrobial ability and visible light transmittance. Silver
nanoparticles (AgNPs), as a nonspecific antimicrobial component, have been loaded
onto contact lenses for the treatment of bacterial and fungal keratitis. Recently,
it was reported that, via a simple immersion method, AgNPs can be synthesized and
fixed onto the surface of polydopamine (PDA)-coated materials. However, in this
study, we found that the above traditional method has the disadvantages of poor
AgNP loading and low visible light transmittance, which could be induced by a
limited amount of phenolic hydroxyl groups on and second oxidation of the PDA
coating, respectively. To overcome these disadvantages, in this paper, we provided
a facile and novel method to robustly bind multilayer-AgNPs on contact lens
surfaces by using dopamine as a reducing agent and bioglue. In comparing with the
monolayer-AgNP-loaded contact lenses fabricated by the traditional method, the
multilayer-AgNP-loaded contact lenses had excellent antimicrobial ability and
better visible light transmittance. Moreover, the multilayer-AgNP-loaded contact
lens had low cytotoxicity to human corneal epithelial cells and anti-inflammation
properties. Furthermore, the shortcoming of decreasing visible light transmittance
induced by excess adherence of AgNPs on the multilayer-AgNP-loaded contact lens was
alleviated by decreasing the size of AgNPs through altering the concentration of
dopamine and AgNO3. Contact lenses loaded with small AgNPs (Ag@PDA-2.5, diameter ≈
25-50 nm) had approximately the same Ag+ release and antimicrobial abilities, but
significantly better visible light transmittance and anti-inflammatory properties
than the contact lenses loaded with large AgNPs (Ag@PDA-5, diameter ≈ 50-75 nm).
After that, in vivo testing indicated the promising therapeutic strategy of
multilayer-AgNP-loaded contact lenses (Ag@PDA-2.5) for early bacterial keratitis
and fungal keratitis. In addition, PDA coating could provide reactive sites to
immobilize other biomolecules or drugs on this multilayer-AgNP-loaded contact lens
for further combination therapies in treating bacterial or fungal keratitis.
Finally, the stability of the visible light transmittance of the multilayer-AgNP-
loaded contact lens was detected. The visible light transmittance of Ag@PDA-2.5 was
weakened after being cultured with an extremely high concentration of bacteria,
while it was stable in the moderate work environment. Though PDA coating had been
wildly used to modify implantation devices, however, few studies about PDA coating
modified contact lenses have been reported so far. Therefore, this research also
provides an important basis for using PDA coating to modify a therapeutic contact
lens. Copyright © 2018 American Chemical Society.
AU - Liu, X.
AU - Chen, J.
AU - Qu, C.
AU - Bo, G.
AU - Jiang, L.
AU - Zhao, H.
AU - Zhang, J.
AU - Lin, Y.
AU - Hua, Y.
AU - Yang, P.
AU - Huang, N.
AU - Yang, Z.
DB - Scopus
IS - 5
KW - antimicrobial
contact lens
dopamine
multilayer-AgNPs
visible light transmittance
Amines
Coated materials
Coatings
Drug delivery
Fungi
Light
Microorganisms
Multilayers
Neurophysiology
phenol
polydopamine
polymer
silver
silver nanoparticle
unclassified drug
Antimicrobial ability
Human corneal epithelial cells
Phenolic hydroxyl group
Therapeutic contact lens
Visible light transmittances
animal experiment
animal model
animal tissue
Article
bacterial keratitis
controlled study
cornea epithelium
cytotoxicity
human
human cell
hydrophilicity
immobilization
in vitro study
in vivo study
keratomycosis
Leporidae
light
material coating
molecular stability
nanofabrication
nonhuman
oxidation
particle size
priority journal
Contact lenses
M3 - Article
PY - 2018
SP - 1568-1579
ST - A Mussel-Inspired Facile Method to Prepare Multilayer-AgNP-Loaded Contact
TI - A Mussel-Inspired Facile Method to Prepare Multilayer-AgNP-Loaded Contact
85047085970&doi=10.1021%2facsbiomaterials.7b00977&partnerID=40&md5=8d900f31a87f2c52
5999934258b59091
VL - 4
ID - 5516
ER -
TY - JOUR
AB - The secretome secreted by stem cells and bioactive material has emerged as a
promising therapeutic choice for traumatic brain injury (TBI). We aimed to
determine the effect of 3D-printed collagen/chitosan/secretome derived from human
umbilical cord blood mesenchymal stem cells scaffolds (3D-CC-ST) on the injured
tissue regeneration process. 3D-CC-ST was performed using 3D printing technology at
a low temperature (-20°C), and the physical properties and degeneration rate were
measured. The utilization of low temperature contributed to a higher
cytocompatibility of fabricating porous 3D architectures that provide a homogeneous
distribution of cells. Immediately after the establishment of the canine TBI model,
3D-CC-ST and 3D-CC (3D-printed collagen/chitosan scaffolds) were implanted into the
cavity of TBI. Following implantation of scaffolds, neurological examination and
motor evoked potential detection were performed to analyze locomotor function
recovery. Histological and immunofluorescence staining were performed to evaluate
neuro-regeneration. The group treated with 3D-CC-ST had good performance of
behavior functions. Implanting 3D-CC-ST significantly reduced the cavity area,
facilitated the regeneration of nerve fibers and vessel reconstruction, and
promoted endogenous neuronal differentiation and synapse formation after TBI. The
implantation of 3D-CC-ST also markedly reduced cell apoptosis and regulated the
level of systemic inflammatory factors after TBI. © 2022 The Author(s). Published
by Oxford University Press.
AU - Liu, X.
AU - Zhang, G.
AU - Wei, P.
AU - Zhong, L.
AU - Chen, Y.
AU - Zhang, J.
AU - Chen, X.
AU - Zhou, L.
C7 - rbac043
DB - Scopus
DO - 10.1093/rb/rbac043
KW - canines
chitosan
collagen
low temperature extrusion 3D printing
secretome
3D modeling
Brain
Cell culture
Cell death
Collagen
Image reconstruction
Neurons
Scaffolds (biology)
Stem cells
Temperature
Three dimensional computer graphics
Tissue regeneration
cell protein
interleukin 10
interleukin 6
microtubule associated protein 2
tuj 1 protein
unclassified drug
3-D printing
3D-printing
Canine
Chitosan scaffold
Low temperature extrusion
Low temperature extrusion 3d printing
Lows-temperatures
Neural-networks
Secretome
Traumatic Brain Injuries
animal experiment
animal model
animal tissue
Article
brain histology
brain tissue
canine model
cell adhesion
cell proliferation
cell survival
cell viability
controlled study
female
human
human cell
immunofluorescence
implantation
in vitro study
in vivo study
locomotion
low temperature
Masson staining
motor evoked potential
MTT assay
myelin sheath
nerve fiber regeneration
neuroapoptosis
nonhuman
porosity
remyelinization
silver staining
stereomicroscopy
synapse
tissue engineering
tissue regeneration
umbilical cord mesenchymal stem cell
water absorption
Young modulus
3D printers
M3 - Article
PY - 2022
ST - Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs
scaffolds for efficient neural network reconstruction in canines with traumatic
brain injury
T2 - Regenerative Biomaterials
TI - Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs
scaffolds for efficient neural network reconstruction in canines with traumatic
brain injury
85139950094&doi=10.1093%2frb
%2frbac043&partnerID=40&md5=95b1dfea38e6a5140f37c84ef6c38475
VL - 9
ID - 5129
ER -
TY - JOUR
AB - The treatment of diabetic wounds remains a global challenge. Compared with
traditional wound dressings, there are higher requirements of antibacterial, anti-
inflammatory and pro-angiogenic effects in diabetic wound dressings. Furthermore,
it is desirable for dressings to self-adapt to wounds with different morphologies
without extra processes and stably (suitable adhesive and self-healing abilities)
provide a conducive environment for wound healing. Herein, we construct an
injectable and self-healing hydrogel through the combination of chitosan (CS) and
metal ions to efficiently improve infected and diabetic wound healing. Benefiting
from the amino and hydroxy groups, the CS molecular chains are cross-linked with
silver ions (Ag+) and copper ions (Cu2+) to promote the formation of the CS-Ag-Cu
hydrogel, which releases Ag+ (an antibacterial agent) and Cu2+ (an angiogenic
agent) over a prolonged period. Moreover, the hydrogel possesses appropriate
adhesive ability, good water absorption ability, antibacterial capability and
biocompatibility according to in vitro investigations. In vivo experimental results
further prove that the CS-Ag-Cu hydrogel can dramatically accelerate tissue repair
in a Staphylococcus aureus (S. aureus)-infected skin incision model in normal rats
and diabetic wounds. © 2022 The Royal Society of Chemistry.
AU - Liu, X.
AU - Zhou, S.
AU - Cai, B.
AU - Wang, Y.
AU - Deng, D.
AU - Wang, X.
DB - Scopus
DO - 10.1039/d2bm00224h
IS - 13
KW - Adhesives
Animals
Chitosan
Diabetes Mellitus
Hydrogels
Rats
Wound Healing
Bacteria
Biocompatibility
Metal ions
Metals
Self-healing materials
Water absorption
chitosan
chitosan silver copper ion hydrogel
copper ion
glucose
hydrogel
hydroxyl group
rhodamine B
silver nitrate
unclassified drug
adhesive agent
antiinfective agent
Ag +
Anti-inflammatories
Antibacterials
Diabetic wounds
Global challenges
Injectables
Property
Self-healing
Wound dressings
Wound healing
angiogenesis
animal experiment
animal model
animal tissue
Article
bacterial viability
biocompatibility
cell migration
cell viability
controlled study
cytotoxicity
diabetic wound
glucose blood level
human
human cell
in vitro study
in vivo study
insulin dependent diabetes mellitus
male
nonhuman
rat
skin biopsy
skin incision
Staphylococcus aureus infection
tissue repair
tubulogenesis
water absorption
wound healing
animal
diabetes mellitus
pharmacology
M3 - Article
PY - 2022
SP - 3480-3492
ST - An injectable and self-healing hydrogel with antibacterial and angiogenic
T2 - Biomaterials Science
TI - An injectable and self-healing hydrogel with antibacterial and angiogenic
85133103101&doi=10.1039%2fd2bm00224h&partnerID=40&md5=8e666243e3e504ce4b7b28e866941
d83
VL - 10
ID - 5121
ER -
TY - JOUR
AB - The formulation and characterization of novel cardiovascular supplements and
drugs without any side effects are in both developed and developing countries
research priority. Myocardial ischemia-reperfusion causes to an inflammatory
response that leads more damage to apoptosis. Chronic and acute immune responses
elicited by myocardial ischemia is in the heart functional deterioration. In this
study, we formulated a modern cardioprotective protective drug by gold
nanoparticles containing Silybum marianum on isoproterenol-induced myocardial
ischemia mice by investigating the PPAR-gamma/NF-kappa B pathway. Determining the
antioxidant capacities of gold nanoparticles was done with the conventional free
radical scavenging test, i.e., DPPH in the presence of butylated hydroxytoluene as
the positive control. The gold nanoparticles were characterized by standard
physicochemical techniques, including FT-IR, FE-SEM, UV-Vis, and TEM. The
nanoparticles inhibited half of the DPPH molecules in the concentration of 72 mu
g/mL. In the in vivo design, to induce myocardial ischemia in C57BL/6 mice,
isoproterenol (40 mg/kg) was administered. The mice were randomly divided into five
groups: (1) control; (2) isoproterenol; (3-5) isoproterenol + gold nanoparticles at
different doses (10, 20 and 40 mu g/ml) and timings. After treatment, cardiac
function was evaluated by histochemical and biochemical analysis. Gold
nanoparticles treatment decreased the inflammatory milieu in the myocardial
ischemia mice heart, thereby blocking the proinflammatory cytokines upregulation
(IL-1 ss, TNF-alpha and IL-6). Also, gold nanoparticles treatment significantly
ameliorate ventricular wall ischemia, reduces the mortality incidence, and inhibits
the myocardial injury markers levels. In addition, gold nanoparticles
administration significantly prevents the typical ST segment depression. Treatment
with gold nanoparticles significantly suppresses the inflammation cytokines
expression and decrease cell death. The gold nanoparticles beneficial effects is
related to the normalization in PPAR-gamma and PPAR-gamma/NF-kappa B/I kappa B-
alpha/IKK alpha/ss phosphorylation gene expression. Gold nanoparticles exert
cardioprotective properties against isoproterenol-induced myocardial ischemia in
mice, which may associated to the PPAR-gamma activation and NF-kappa B signaling
inhibition.
AN - WOS:001022159700001
AU - Liu, Y.
AU - Guo, J.
AU - Zhou, Z.
AU - Wu, Q. K.
AU - Jin, X.
AU - Wang, T.
C6 - JUN 2023
C7 - 110902
DA - AUG
DO - 10.1016/j.inoche.2023.110902
PY - 2023
SN - 1387-7003
1879-0259
ST - Protective properties of nanoparticles green-synthesized by plant on
myocardial ischemia
T2 - INORGANIC CHEMISTRY COMMUNICATIONS
TI - Protective properties of nanoparticles green-synthesized by plant on
myocardial ischemia
VL - 154
ID - 6719
ER -
TY - JOUR
AB - Background: Gold nanoparticles (AuNPs) have potential applications in the
treatment and diagnosis process, which are attributed to their biocompatibility and
high efficiency of drug delivery. In the current study, we utilized an extract of
Euphrasia o/ficinalis, a traditional folk medicine, to synthesize gold
nanoparticles (EO-AuNPs), and investigated their anti-inflammatory effects on
lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Materials and methods:
The AuNPs were synthesized from an ethanol extract of E. officinalis leaves and
characterized using several analytical techniques. Anti-inflammatory activities of
EO-AuNPs were detected by a model of LPS-induced upregulation of inflammatory
mediators and cytokines including nitric oxide (NO), inducible nitric oxide
synthase (iNOS), tumor necrosis factor-alpha (TNF-alpha), IL-1 beta, and IL-6 in
RAW 264.7 cells. The activation of nuclear factor (NF)-kappa B and Janus
kinase/signal transducer and activators of transcription (JAK/STAT) signaling
pathways was investigated by Western blot. Results: The results confirmed the
successful synthesis of AuNPs by E. officinalis. Transmission electron microscopy
images showed obvious uptake of EO-AuNPs and internalization into intracellular
membrane-bound compartments, resembling endosomes and lysosomes by RAW 264.7 cells.
Cell viability assays showed that EO-AuNPs exhibited little cytotoxicity in RAW
264.7 cells at 100 mu g/mL concentration after 24 hours. EO-AuNPs significantly
suppressed the LPS-induced release of NO, TNF-alpha, IL-1 beta, and IL-6 as well as
the expression of the iNOS gene and protein in RAW 264.7 cells. Further experiments
demonstrated that pretreatment with EO-AuNPs significantly reduced the
phosphorylation and degradation of inhibitor kappa B-alpha and inhibited the
nuclear translocation of NF-kappa B p65. In addition, EO-AuNPs suppressed LPS-
stimulated inflammation by blocking the activation of JAK/STAT pathway. Conclusion:
The synthesized EO-AuNPs showed anti-inflammatory activity in LPS-induced RAW 264.7
cells, suggesting they may be potential candidates for treating inflammatory-
mediated diseases.
AN - WOS:000467080700003
AU - Liu, Y.
AU - Kim, S.
AU - Kim, Y. J.
AU - Perumalsamy, H.
AU - Lee, S.
AU - Hwang, E.
AU - Yi, T. H.
DO - 10.2147/IJN.S199781
PY - 2019
SN - 1178-2013
SP - 2945-2959
ST - Green synthesis of gold nanoparticles using Euphrasia officinalis leaf
extract to inhibit lipopolysaccharide-induced inflammation through NF-kappa B and
JAK/STAT pathways in RAW 264.7 macrophages
TI - Green synthesis of gold nanoparticles using Euphrasia officinalis leaf
extract to inhibit lipopolysaccharide-induced inflammation through NF-kappa B and
JAK/STAT pathways in RAW 264.7 macrophages
VL - 14
ID - 6394
ER -
TY - JOUR
AB - As a widely applied nanomaterial, silver nanomaterials (AgNMs) have increased
public concern about their potential adverse biological effects. However, there are
few related researches on the long-term toxicity, especially on the reversibility
of AgNMs in vivo. In the current study, this issue was tackled by exploring liver
damage after an intravenous injection of silver nanorods with golden cores
(Au@AgNRs) and its potential recovery in a relatively long term (8 w). After the
administration of Au@AgNRs into rats, Ag was found to be rapidly cleared from blood
within 10 min and mainly accumulated in liver as well as spleen until 8 w. All
detected parameters almost displayed a two-stage response to Au@AgNRs
administration, including biological markers, histological changes and metabolic
variations. For the short-term (2 w) responses, some toxicological parameters
(hematological changes, cytokines, liver damages etc.) significantly changed
compared to control and AuNRs group. However, after a 6-week recovery, all
abovementioned changes mostly returned to the normal levels in the Au@AgNRs group.
These indicated that after a lengthy period, acute bioeffects elicited by AgNMs
could be followed by the adaptive recovery, which will provide a novel and valuable
toxicity mechanism of AgNMs for potential biomedical applications of AgNMs.
AN - WOS:000726849900001
AU - Liu, Y.
AU - Wen, H. R.
AU - Wu, X. C.
AU - Wu, M. Y.
AU - Liu, L.
AU - Wang, J. H.
AU - Huo, G. T.
AU - Lyu, J. J.
AU - Xie, L. M.
AU - Dan, M.
C7 - 2656
DA - OCT
DO - 10.3390/nano11102656
IS - 10
PY - 2021
SN - 2079-4991
ST - The Bio-Persistence of Reversible Inflammatory, Histological Changes and
Metabolic Profile Alterations in Rat Livers after Silver/Gold Nanorod
Administration
T2 - NANOMATERIALS
TI - The Bio-Persistence of Reversible Inflammatory, Histological Changes and
Metabolic Profile Alterations in Rat Livers after Silver/Gold Nanorod
Administration
VL - 11
ID - 6255
ER -
TY - JOUR
AB - A facile and general strategy for preparing uniform and multifunctional
polyphenol-based colloidal particles through amine-catalyzed polymerization-induced
self-assembly is described. The size and interfacial adhesion of polyphenol spheres
can be easily controlled over a wide range via adjusting the concentration of the
cosolvent and monomer. Moreover, the polyphenol spheres showed excellent thermal
and chemical stability and highly active properties and could efficiently deplete
the reactive oxygen species (ROS), which are helpful for in vivo ROS regulation for
inflammatory therapeutic. The accessible and versatile method provides a feasible
way for the rational engineering of multifunctional polyphenol spheres, which have
great potential in many fields. © 2021 American Chemical Society.
AU - Liu, Z.
AU - Yu, W.
AU - Sheng, W.
AU - Li, R.
AU - Guo, H.
AU - Feng, X.
AU - Li, Q.
AU - Wang, R.
AU - Li, W.
AU - Jia, X.
DB - Scopus
DO - 10.1021/acs.biomac.1c01158
IS - 1
KW - Amines
Catalysis
Polymerization
Polyphenols
Chemical stability
Self assembly
4 nitrophenol
amine
catechol
dextran sulfate
gold nanoparticle
mesalazine
monomer
phenol
phloroglucinol
poly(phloroglucinol)
polyphenol
pyrogallol
Schiff base
self assembled nanoparticle
silver nanoparticle
unclassified drug
Catalyzed polymerization
Colloidal particle
Controllable synthesis
Cosolvents
In-vivo
Interfacial adhesions
Property
Thermal and chemical stabilities
animal experiment
animal model
animal tissue
Article
controlled study
cross linking
cytotoxicity
drug stability
EC50
histology
hydrogen bond
male
Michael addition
mouse
nonhuman
polymerization
thermostability
ulcerative colitis
ultrasound assisted extraction
zeta potential
catalysis
Spheres
M3 - Article
PY - 2022
SP - 140-149
ST - Controllable Synthesis of Polyphenol Spheres via Amine-Catalyzed
Polymerization-Induced Self-Assembly
T2 - Biomacromolecules
TI - Controllable Synthesis of Polyphenol Spheres via Amine-Catalyzed
Polymerization-Induced Self-Assembly
85122008165&doi=10.1021%2facs.biomac.1c01158&partnerID=40&md5=709b6b94af362ab1b1e53
27e3eeb1c85
VL - 23
ID - 5097
ER -
TY - JOUR
AB - Inflammation is one of the major toxic effects reported in response to in
vitro or in vivo nanoparticle (NP) exposure. Among engineered NPs, silver
nanoparticles (AgNPs) are very attractive for the development of therapeutic
strategies, especially because of their antimicrobial properties. In humans,
neutrophils, key players in inflammation, are the most abundant blood leukocytes
that spontaneously undergo apoptosis, a central cell death mechanism regulating
inflammation. The aim of this study was to evaluate the effect of AgNPs on
neutrophil apoptosis. Transmission electronic microscopy reveals that AgNPs rapidly
penetrate inside neutrophils. AgNPs induced atypical cell death where the cell
volume increased and the cell surface expression of CD16 remained unaltered unlike
apoptotic neutrophils where cell shrinkage and loss of CD16 are typically observed.
The AgNP-induced atypical cell death is distinct from necrosis and reversed by a
pancaspase inhibitor or by inhibitors of the inflammatory caspase-1 and caspase-4.
In addition, AgNPs induced IL-1β production inhibited by caspase-1 and caspase-4
inhibitors and also induced caspase-1 activity. Reactive oxygen species (ROS)
production was increased by AgNPs and the atypical cell death was inhibited by the
antioxidant n-acetylcysteine. Under similar experimental conditions, adhesion of
neutrophils leads to neutrophil extracellular trap (NET) release induced by AgNPs.
However, this process was not reversed by caspase inhibitors. We conclude that
AgNPs rapidly induced an atypical cell death in neutrophils by a mechanism
involving caspase-1, -4 and ROS. However, in adherent neutrophils, AgNPs induced
NET release and, therefore, are novel agents able to trigger NET release. © 2015
Elsevier B.V.
AU - Liz, R.
AU - Simard, J. C.
AU - Leonardi, L. B. A.
AU - Girard, D.
DB - Scopus
DO - 10.1016/j.intimp.2015.06.030
IS - 1
KW - Apoptosis
Granulocytes
Inflammation
Nanotoxicology
Caspases
Cell Adhesion
Cell Death
Cell Size
Cells, Cultured
Extracellular Traps
Humans
Interleukin-1beta
Metal Nanoparticles
Neutrophils
Silver
acetylcysteine
antioxidant
caspase
caspase 4
caspase inhibitor
CD16 antigen
interleukin 1beta
silver nanoparticle
IL1B protein, human
metal nanoparticle
silver
antigen expression
apoptosis
Article
cell adhesion
cell death
cell surface
cell volume
controlled study
cytokine production
enzyme activity
enzyme inhibition
extracellular trap
human
human cell
necrosis
neutrophil
priority journal
cell culture
cell size
drug effects
metabolism
physiology
M3 - Article
PY - 2015
SP - 616-625
ST - Silver nanoparticles rapidly induce atypical human neutrophil cell death by a
process involving inflammatory caspases and reactive oxygen species and induce
neutrophil extracellular traps release upon cell adhesion
TI - Silver nanoparticles rapidly induce atypical human neutrophil cell death by a
process involving inflammatory caspases and reactive oxygen species and induce
neutrophil extracellular traps release upon cell adhesion
84938304630&doi=10.1016%2fj.intimp.2015.06.030&partnerID=40&md5=ae65e7eae3c2182f48c
d76484f2c9af5
VL - 28
ID - 5620
ER -
TY - JOUR
AB - Background: With the continued integration of engineered nanomaterials (ENMs)
into everyday applications, it is important to understand their potential for
inducing adverse human health effects. However, standard in vitro hazard
characterisation approaches suffer limitations for evaluating ENM and so it is
imperative to determine these potential hazards under more physiologically relevant
and realistic exposure scenarios in target organ systems, to minimise the necessity
for in vivo testing. The aim of this study was to determine if acute (24 h) and
prolonged (120 h) exposures to five ENMs (TiO2, ZnO, Ag, BaSO4 and CeO2) would have
a significantly different toxicological outcome (cytotoxicity, (pro-)inflammatory
and genotoxic response) upon 3D human HepG2 liver spheroids. In addition, this
study evaluated whether a more realistic, prolonged fractionated and repeated ENM
dosing regime induces a significantly different toxicity outcome in liver spheroids
as compared to a single, bolus prolonged exposure. Results: Whilst it was found
that the five ENMs did not impede liver functionality (e.g. albumin and urea
production), induce cytotoxicity or an IL-8 (pro-)inflammatory response, all were
found to cause significant genotoxicity following acute exposure. Most
statistically significant genotoxic responses were not dose-dependent, with the
exception of TiO2. Interestingly, the DNA damage effects observed following acute
exposures, were not mirrored in the prolonged exposures, where only 0.2–5.0 µg/mL
of ZnO ENMs were found to elicit significant (p ≤ 0.05) genotoxicity. When
fractionated, repeated exposure regimes were performed with the test ENMs, no
significant (p ≥ 0.05) difference was observed when compared to the single, bolus
exposure regime. There was < 5.0% cytotoxicity observed across all exposures, and
the mean difference in IL-8 cytokine release and genotoxicity between exposure
regimes was 3.425 pg/mL and 0.181%, respectively. Conclusion: In conclusion, whilst
there was no difference between a single, bolus or fractionated, repeated ENM
prolonged exposure regimes upon the toxicological output of 3D HepG2 liver
spheroids, there was a difference between acute and prolonged exposures. This study
highlights the importance of evaluating more realistic ENM exposures, thereby
providing a future in vitro approach to better support ENM hazard assessment in a
routine and easily accessible manner.[Figure not available: see fulltext.] © 2021,
The Author(s).
AU - Llewellyn, S. V.
AU - Conway, G. E.
AU - Zanoni, I.
AU - Jørgensen, A. K.
AU - Shah, U. K.
AU - Seleci, D. A.
AU - Keller, J. G.
AU - Kim, J. W.
AU - Wohlleben, W.
AU - Jensen, K. A.
AU - Costa, A.
AU - Jenkins, G. J. S.
AU - Doak, S. H.
C7 - 193
DB - Scopus
DO - 10.1186/s12951-021-00938-w
IS - 1
KW - Engineered nanomaterials
Genotoxicity
In vitro liver models
Nanotoxicology
Physiologically relevant exposure
Albumins
Cell Proliferation
Cytokines
DNA Damage
Hep G2 Cells
Humans
Hydrophobic and Hydrophilic Interactions
Liver
Mutagenicity Tests
Nanostructures
Particle Size
Urea
Barite
Barium sulfate
Cerium oxide
Cytotoxicity
Hazards
Silver compounds
Titanium dioxide
Zinc oxide
5,5 dimethyl 1 pyrroline 1 oxide
aflatoxin B1
albumin
alcohol
barium sulfate
bromocresol green
carbon dioxide
carcinogen
cerium oxide
copper
edetic acid
glucose
glutamine
gold
helium
horseradish peroxidase
hydroxylamine
interleukin 6
interleukin 8
nanomaterial
nitric acid
nitrogen
nitrone
oxygen
phosphate buffered saline
polysorbate 20
potassium chloride
silicon
silver
streptavidin
streptomycin
superoxide
titanium dioxide
trypsin
urea
water
zinc oxide
albuminoid
cytokine
Genotoxic response
Hazard Assessment
Human health effects
Potential hazards
Realistic exposure scenario
Urea production
Article
cell culture
cell suspension
cytokinesis
cytotoxicity
DNA damage
drug exposure
electrophoretic mobility
fetal bovine serum
genotoxicity
Hep-G2 cell line
hydrophilicity
hydrophobicity
incubation time
liver
micronucleus test
nonhuman
particle size
physical chemistry
relative humidity
trypan blue assay
ultrasound
vacuum
wavelength dispersive X ray fluorescence spectroscopy
zeta potential
cell proliferation
chemical phenomena
drug effect
human
metabolism
mutagen testing
pathology
Oxide minerals
M3 - Article
PY - 2021
ST - Understanding the impact of more realistic low-dose, prolonged engineered
nanomaterial exposure on genotoxicity using 3D models of the human liver
TI - Understanding the impact of more realistic low-dose, prolonged engineered
nanomaterial exposure on genotoxicity using 3D models of the human liver
85109028240&doi=10.1186%2fs12951-021-00938-
w&partnerID=40&md5=706c1738d37d6ec48fb74b817830a215
VL - 19
ID - 5190
ER -
TY - JOUR
AB - Silver is a metal with well-known antibacterial effects. This makes silver an
attractive coating material for medical devices for use inside the body, e.g.
orthopaedic prostheses and catheters used in neurosurgery as it has been found to
reduce the high risk of infections. Lately, the use of nano-silver particles in the
industry, e.g. woven into fabrics and furniture has increased, and thus the
exposure to silver particles in daily life increases. To study the effect of
metallic silver particles on nervous tissue, we injected micron-sized silver
particles into the mouse brain by stereotactic procedures. After 7, 14 days and 9
months, the silver-exposed animals had considerable brain damage seen as cavity
formation and inflammation adjacent to the injected metallic silver particles. The
tissue loss involved both cortical and hippocampal structures and resulted in
enlargement of the lateral ventricles. Autometallographic silver enhancement showed
silver uptake in lysosomes of glia cells and neurons in the ipsilateral cortex and
hippocampus alongside a minor uptake on the contralateral side. Silver was also
detected in ependymal cells and the choroid plexus. After 9months, spreading of
silver to the kidneys was seen. Cell counts of immunostained sections showed that
metallic silver induced a statistically significant inflammatory response, i.e.
increased microgliosis (7days: p<0.0001; 14days: p<0.01; 9months: p<0.0001) and
TNF-α expression (7 and 14days: p<0.0001; 9months: p=0.91). Significant
astrogliosis (7, 14days and 9months: p<0.0001) and increased metallothionein (MT
I+II) expression (7 and 14days: p<0.0001; 9months: p<0.001) were also seen in
silver-exposed brain tissue. We conclude that metallic silver implants release
silver ions causing neuroinflammation and a progressive tissue loss in the brain. ©
2011 The Authors. Basic & Clinical Pharmacology & Toxicology © 2011 Nordic
Pharmacological Society.
AU - Locht, L. J.
AU - Pedersen, M. O.
AU - Markholt, S.
AU - Bibby, B. M.
AU - Larsen, A.
AU - Penkowa, M.
AU - Stoltenberg, M.
AU - Rungby, J.
DB - Scopus
DO - 10.1111/j.1742-7843.2010.00668.x
IS - 1
KW - Animals
Brain
Cell Count
Female
Inflammation
Metallothionein
Mice
Mice, Inbred BALB C
Silver
Stereotaxic Techniques
Time Factors
Tissue Distribution
Animalia
metal
metallothionein I
metallothionein II
silver
animal cell
animal experiment
animal tissue
animal welfare
apoptosis
article
astrocytosis
autometallography
brain cortex
brain damage
brain nerve cell
brain tissue
brain ventricle
cell count
choroid plexus
controlled study
correlation analysis
encephalitis
ependyma cell
exposure
female
glia cell
gliosis
hippocampus
kidney
lysosome
mouse
nervous tissue
nick end labeling
nonhuman
priority journal
protein expression
stereotactic procedure
M3 - Article
PY - 2011
SP - 1-10
ST - Metallic silver fragments cause massive tissue loss in the mouse brain
T2 - Basic and Clinical Pharmacology and Toxicology
TI - Metallic silver fragments cause massive tissue loss in the mouse brain
79958859539&doi=10.1111%2fj.1742-
7843.2010.00668.x&partnerID=40&md5=c0838e9f43cdebc08bca3c30b86c8038
VL - 109
ID - 5671
ER -
TY - JOUR
AB - In clinical medicine metallic silver is used as anti-bacterial coating on
various catheters, bandages and prostheses. By means of dissolucytosis, i.e.
extracellular macrophage-mediated bio-liberation of metal ions, silver ions are
continuously liberated from silver surfaces starting within minutes of exposure.
The present study investigates how bio-liberation and subsequent cellular uptake of
silver ions affects cell viability and cell signalling within the first 3-24 hours
of exposure when J774 macrophages are grown directly on a silver surface.
Autometallography (AMG) was applied to demonstrate cytoplasmatic silver uptake and
localisation after 1, 3, 12 and 24 hours of exposure to metallic silver. From 12
hours onwards the cells were completely filled with silver enhanced silver-sulphur
nanocrystals (AMG-silver grains). At the ultrastructural level, the silver
accumulations were located to lysosome-like structures. An immunoassay cell death
kit found silver-induced apoptosis after 12 and 24 hours of exposure. Necrosis was
seen at the same times. Judged by mRNA analysis silver exposure statistically
significantly induces TNF-α and m-CSF gene expression, especially at 3 hours.
Furthermore, anti-inflammatory IL-10 transcription is reduced by silver uptake and
24 hours of silver exposure induces massive iNOS-2 gene expression. At the same
time silver exposure increases the gene expression of metallothionein (MT-I/MT-II),
a cystein-rich protein known for its role in detoxifying heavy metals. Our data
suggest that silver ions liberated from metallic silver surfaces accumulate in
lysosomes, reduce macrophage viability by apoptosis and necrosis and induce a
proinflammatory response.
AU - Locht, L. J.
AU - Smidt, K.
AU - Rungby, J.
AU - Stoltenberg, M.
AU - Larsen, A.
DB - Scopus
IS - 6
KW - Apoptosis
Autometallography (AMG)
Dissolucytosis
Inflammation
Metallic silver
Animals
Cell Line
Macrophages
Mice
Silver
silver
animal
apoptosis
article
cell line
drug effect
inflammation
macrophage
metabolism
mouse
ultrastructure
M3 - Article
PY - 2011
SP - 689-697
ST - Uptake of silver from metallic silver surfaces induces cell death and a pro-
inflammatory response in cultured J774 macrophages
T2 - Histology and Histopathology
TI - Uptake of silver from metallic silver surfaces induces cell death and a pro-
inflammatory response in cultured J774 macrophages
79959900371&partnerID=40&md5=0bf268d8af309811f1ab1187deb3937a
VL - 26
ID - 5713
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are commonly used in commercial and medical
applications. However, AgNPs may induce toxicity, extracellular matrix (ECM)
changes and inflammatory responses. Fibroblasts are key players in remodeling
processes and major producers of the ECM. The aims of this study were to explore
the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung
slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory
responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different
sizes, 10 nm and 75 nm, at concentrations 2 µg/mL and 10 µg/mL. Changes in
synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1.
Ag10 and Ag75 affected cell viability, with significantly reduced metabolic
activities at 10 µg/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly
increased procollagen I synthesis and release of IL-8, prostaglandin E2, RANTES and
eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our data
indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro with
altered procollagen and proinflammatory cytokine secretion in fibroblasts over
time. Hence, careful characterizations of AgNPs are of importance, and future
studies should include timepoints beyond 24 h. © 2020 by the authors. Licensee
AU - Löfdahl, A.
AU - Jern, A.
AU - Flyman, S.
AU - Kåredal, M.
AU - Larsson-Callerfelt, A. K.
C7 - 1868
DB - Scopus
DO - 10.3390/nano10091868
IS - 9
KW - Cytokines
Extracellular matrix
Growth factors
Human lung fibroblasts
Precision-cut lung slices
Procollagen
Toxicity
M3 - Article
PY - 2020
SP - 1-12
ST - Silver nanoparticles alter cell viability ex vivo and in vitro and induce
proinflammatory effects in human lung fibroblasts
T2 - Nanomaterials
TI - Silver nanoparticles alter cell viability ex vivo and in vitro and induce
proinflammatory effects in human lung fibroblasts
85091518379&doi=10.3390%2fnano10091868&partnerID=40&md5=aa5b5c2033cd66266e2845a9ca4
ecf69
VL - 10
ID - 5347
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are commonly used in commercial and medical
applications. However, AgNPs may induce toxicity, extracellular matrix (ECM)
changes and inflammatory responses. Fibroblasts are key players in remodeling
processes and major producers of the ECM. The aims of this study were to explore
the effect of AgNPs on cell viability, both ex vivo in murine precision cut lung
slices (PCLS) and in vitro in human lung fibroblasts (HFL-1), and immunomodulatory
responses in fibroblasts. PCLS and HFL-1 were exposed to AgNPs with different
sizes, 10 nm and 75 nm, at concentrations 2 mu g/mL and 10 mu g/mL. Changes in
synthesis of ECM proteins, growth factors and cytokines were analyzed in HFL-1.
Ag10 and Ag75 affected cell viability, with significantly reduced metabolic
activities at 10 mu g/mL in both PCLS and HFL-1 after 48 h. AgNPs significantly
increased procollagen I synthesis and release of IL-8, prostaglandin E-2, RANTES
and eotaxin, whereas reduced IL-6 release was observed in HFL-1 after 72 h. Our
data indicate toxic effects of AgNP exposure on cell viability ex vivo and in vitro
with altered procollagen and proinflammatory cytokine secretion in fibroblasts over
time. Hence, careful characterizations of AgNPs are of importance, and future
studies should include timepoints beyond 24 h.
AN - WOS:000581260400001
AU - Lofdahl, A.
AU - Jern, A.
AU - Flyman, S.
AU - Karedal, M.
AU - Larsson-Callerfelt, A. K.
C7 - 1868
DA - SEP
DO - 10.3390/nano10091868
IS - 9
PY - 2020
SN - 2079-4991
ST - Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce
Proinflammatory Effects in Human Lung Fibroblasts
T2 - NANOMATERIALS
TI - Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce
Proinflammatory Effects in Human Lung Fibroblasts
VL - 10
ID - 5987
ER -
TY - JOUR
AB - Bioactive gel-glasses, such as the silver-doped Ag-S70C30 glass, can be used
to modify the inflammatory response in a local body compartment such as in acne
lesions and in non-healing dermal wounds. In this study, the cytotoxicity of
soluble silver, calcium and silica ions on human epidermal keratinocytes was
investigated by measurements of mitochondrial activity (MTT assay) and neutral red
dye uptake (NR assay). Ag-S70C30 extracts were prepared by soaking glass powder in
complete culture medium at concentrations of 1 mg/ml and 2 mg/ml (mg of glass
powder per ml of culture medium). Silver concentrations for both concentrations of
approximately 1 ppm were detected by inductive coupled plasma analysis (ICP). No
negative effect on the cell viability was measured for an initial gel-glass
concentration of 1 mg/ml and for the two shortest extraction times at a
concentration of 2 mg/ml. Based on the results from MTT/ NR assays, a pH rise of
approximately one unit had no negative effect on the NHEK-A cell viability. This
preliminary study on keratinocyte viability merits future investigations on silver
bioglass as a novel antimicrobial wound healing agent.
AU - Lohbauer, U.
AU - Jell, G.
AU - Saravanapavan, P.
AU - Jones, J. R.
AU - Hench, L. L.
DB - Scopus
DO - 10.4028/0-87849-961-x.431
KW - Bioactive glass
Cytotoxicity
Keratinocytes
Soluble ions
Wound healing
Bioassay
Cells
Doping (additives)
Medical problems
Silver
Soaking glass powders
M3 - Article
PY - 2005
SP - 431-434
ST - Indirect cytotoxicity evaluation of silver doped bioglass Ag-S70C30 on human
primary keratinocytes
T2 - Key Engineering Materials
TI - Indirect cytotoxicity evaluation of silver doped bioglass Ag-S70C30 on human
25844449671&doi=10.4028%2f0-87849-961-
x.431&partnerID=40&md5=6e01709935f7711a0a32ed71433157e6
VL - 284-286
ID - 5826
ER -
TY - CHAP
A2 - Li, P.
A2 - Zhang, K.
A2 - Colwell, C. W.
AB - Bioactive gel-glasses, such as the silver-doped Ag-S70C30 glass, can be used
to modify the inflammatory response in a local body compartment such as in acne
lesions and in non-healing dermal wounds. In this study, the cytotoxicity of
soluble silver, calcium and silica ions on human epidermal keratinocytes was
investigated by measurements of mitochondrial activity (MTT assay) and neutral red
dye uptake (NR assay). Ag-S70C30 extracts were prepared by soaking glass powder in
complete culture medium at concentrations of 1 mg/ml and 2 mg/ml (mg of glass
powder per ml of culture medium). Silver concentrations for both concentrations of
approximately 1 ppm were detected by inductive coupled plasma analysis (ICP). No
negative effect on the cell viability was measured for an initial gel-glass
concentration of 1 mg/ml and for the two shortest extraction times at a
concentration of 2 mg/ml. Based on the results from MTT/NR assays, a pH rise of
approximately one unit had no negative effect on the NHEK-A cell viability. This
preliminary study on keratinocyte viability merits future investigations on silver
bioglass as a novel antimicrobial wound healing agent.
AN - WOS:000228359500106
AU - Lohbauer, U.
AU - Jell, G.
AU - Saravanapavan, P.
AU - Jones, J. R.
AU - Hench, L. L.
DO - 10.4028/www.scientific.net/KEM.284-286.431
PY - 2005
SE - 17th International Symposium on Ceramics in Medicine
SN - 1013-9826
0-87849-961-X
SP - 431-434
ST - Indirect cytotoxicity evaluation of silver doped bioglass Ag-S70C30 on human
T2 - BIOCERAMICS, VOL 17
TI - Indirect cytotoxicity evaluation of silver doped bioglass Ag-S70C30 on human
VL - 284-286
ID - 6199
ER -
TY - JOUR
AB - It is unknown whether the flavonoid rutin can protect the silver catfish
liver in response to exposure to a known stressor, such as the prophylactic usage
of the antimicrobial agent oxytetracycline. Thus, the current study aimed to assess
the effect of rutin incorporation into the silver catfish diet formulation on
oxytetracycline-induced liver oxidative stress and apoptosis. Fish were split into
four groups as follows: control, rutin (1.5 g kg diet−1), oxytetracycline (0.1 g kg
diet−1) and rutin+oxytetracycline (1.5 g kg diet−1 and 0.1 g kg diet−1,
respectively). After two weeks of feeding with the different diets (standard,
rutin-, oxytetracycline and rutin+oxytetracycline-added diets), fish were
euthanized to collect the liver. Although the rutin-added diet was unable to
recover glutathione peroxidase activity, ascorbic acid and reduced glutathione
(GSH) levels, which were depleted due to oxytetracycline consumption, it markedly
diminished the oxidized glutathione (GSSG) content, thus decreasing the GSSG to GSH
ratio, an important index of oxidative stress. It also increased glutathione
reductase and markedly augmented glucose-6-phosphate dehydrogenase activities,
which were declined after oxytetracycline ingestion. Furthermore, the rutin-added
diet reestablished superoxide dismutase and catalase activities and reduced lipid
peroxidation, nitric oxide and superoxide anion levels as well, all changes
resulting from oxytetracycline consumption. Finally, it also prevented
oxytetracycline-induced apoptosis through increasing heat shock protein 70 and
markedly decreasing high mobility group box 1 and, consequently, reducing cleaved
caspase-3 protein levels. Therefore, in conclusion, the incorporation of this
flavonoid to the silver catfish diet protected the liver against oxytetracycline-
induced liver oxidative stress and apoptosis. © 2020 Elsevier Inc.
AU - Londero, É P.
AU - Bressan, C. A.
AU - Pês, T. S.
AU - Saccol, E. M. H.
AU - Finamor, I. A.
C7 - 108848
DB - Scopus
DO - 10.1016/j.cbpc.2020.108848
KW - Antimicrobial
Fish
Flavonoid
Prophylactic
Stressor
Animal Feed
Animals
Antioxidants
Apoptosis
Biomarkers
Catfishes
Liver
Oxidative Stress
Oxytetracycline
Rutin
ascorbic acid
caspase 3
catalase
glucose 6 phosphate dehydrogenase
glutathione
lipid hydroperoxide
nitric oxide
oxytetracycline
rutoside
superoxide
antiinfective agent
antioxidant
biological marker
animal experiment
animal tissue
apoptosis
Article
catfish
controlled study
lipid peroxidation
liver protection
nonhuman
oxidative stress
priority journal
protein cleavage
tissue homogenate
animal
animal food
drug effect
liver
metabolism
pathology
M3 - Article
PY - 2021
ST - Rutin-added diet protects silver catfish liver against oxytetracycline-
induced oxidative stress and apoptosis
TI - Rutin-added diet protects silver catfish liver against oxytetracycline-
induced oxidative stress and apoptosis
85089953226&doi=10.1016%2fj.cbpc.2020.108848&partnerID=40&md5=35ce70f4612598a47e6b4
79ba82545fb
VL - 239
ID - 5326
ER -
TY - JOUR
AB - Concerns about the bioaccumulation and toxicity of gold nanoparticles inside
humans have recently risen. HT-29 and HepG2 cell lines and Wistar rats were exposed
to 10, 30 or 60 nm gold nanoparticles to determine their tissue distribution,
subcellular location and deleterious effects. Cell viability, ROS production and
DNA damage were evaluated in vitro. Lipid peroxidation and protein carbonylation
were determined in liver. ICP-MS measurements showed the presence of gold in
intestine, kidney, liver, spleen, feces and urine. Subcellular locations of gold
nanoparticles were observed in colon cells and liver samples by transmission
electron microscopy. Inflammatory markers in liver and biochemical parameters in
plasma were measured to assess the inflammatory status and presence of tissue
damage. The size of the nanoparticles determined differences in the biodistribution
and the excretion route. The smallest nanoparticles showed more deleterious
effects, confirmed by their location inside the cell nucleus and the higher DNA
damage. (c) 2017 Elsevier Inc. All rights reserved.
AN - WOS:000423842300001
AU - Lopez-Chaves, C.
AU - Soto-Alvaredo, J.
AU - Montes-Bayon, M.
AU - Bettmer, J.
AU - Llopis, J.
AU - Sanchez-Gonzalez, C.
DA - JAN
DO - 10.1016/j.nano.2017.08.011
IS - 1
PY - 2018
SN - 1549-9634
1549-9642
SP - 1-12
ST - Gold nanoparticles: Distribution, bioaccumulation and toxicity. In vitro and
in vivo studies
TI - Gold nanoparticles: Distribution, bioaccumulation and toxicity. In vitro and
in vivo studies
VL - 14
ID - 6316
ER -
TY - JOUR
AB - Background: Animal models remain at that time a reference tool to predict
potential pulmonary adverse effects of nanomaterials in humans. However, in a
context of reduction of the number of animals used in experimentation, there is a
need for reliable alternatives. In vitro models using lung cells represent relevant
alternatives to assess potential nanomaterial acute toxicity by inhalation,
particularly since advanced in vitro methods and models have been developed.
Nevertheless, the ability of in vitro experiments to replace animal experimentation
for predicting potential acute pulmonary toxicity in human still needs to be
carefully assessed. The aim of the study was to evaluate the differences existing
between the in vivo and the in vitro approaches for the prediction of nanomaterial
toxicity and to find advanced methods to enhance in vitro predictivity. For this
purpose, rats or pneumocytes in co-culture with macrophages were exposed to the
same poorly soluble and poorly toxic TiO2 and CeO2 nanomaterials, by the
respiratory route in vivo or using more or less advanced methodologies in vitro.
After 24 h of exposure, biological responses were assessed focusing on pro-
inflammatory effects and quantitative comparisons were performed between the in
vivo and in vitro methods, using compatible dose metrics. Results: For each dose
metric used (mass/alveolar surface or mass/macrophage), we observed that the most
realistic in vitro exposure method, the air-liquid interface method, was the most
predictive of in vivo effects regarding biological activation levels. We also noted
less differences between in vivo and in vitro results when doses were normalized by
the number of macrophages rather than by the alveolar surface. Lastly, although we
observed similarities in the nanomaterial ranking using in vivo and in vitro
approaches, the quality of the data-set was insufficient to provide clear ranking
comparisons. Conclusions: We showed that advanced methods could be used to enhance
in vitro experiments ability to predict potential acute pulmonary toxicity in vivo.
Moreover, we showed that the timing of the dose delivery could be controlled to
enhance the predictivity. Further studies should be necessary to assess if air-
liquid interface provide more reliable ranking of nanomaterials than submerged
methods.
AN - WOS:000434353400001
AU - Loret, T.
AU - Rogerieux, F.
AU - Trouiller, B.
AU - Braun, A.
AU - Egles, C.
AU - Lacroix, G.
C7 - 25
DA - JUN 4
DO - 10.1186/s12989-018-0260-6
PY - 2018
SN - 1743-8977
ST - Predicting the in vivo pulmonary toxicity induced by acute exposure to poorly
soluble nanomaterials by using advanced in vitro methods
TI - Predicting the in vivo pulmonary toxicity induced by acute exposure to poorly
soluble nanomaterials by using advanced in vitro methods
VL - 15
ID - 6458
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are among the most commercialized nanomaterials
in biomedicine due to their antimicrobial and anti-inflammatory properties.
Nevertheless, possible health hazards of exposure to AgNPs are yet to be understood
and therefore raise public concern in regards of their safety. In this study, sex-
related differences, role of steroidal hormones and influence of two different
surface stabilizing agents (polymer vs. protein) on distribution and adverse
effects of AgNPs were investigated in vivo. Intact and gonadectomised male and
female mice were treated with seven AgNPs doses administered intraperitoneally
during 21 days. After treatment, steroid hormone levels in serum, accumulation of
Ag levels and oxidative stress biomarkers in liver, kidneys, brain and lungs were
determined. Sex-related differences were observed in almost all tissues.
Concentration of Ag was significantly higher in the liver of females compared to
males. No significant difference was found for AgNP accumulation in lungs between
females and males, while the lungs of intact males showed significantly higher Ag
accumulation compared to gonadectomised group. Effect of surface coating was also
observed, as Ag accumulation was significantly higher in kidneys and liver of
intact females, as well as in kidneys and brain of intact males treated with
protein-coated AgNPs compared to polymeric AgNPs. Oxidative stress response to
AgNPs was the most pronounced in kidneys where protein-coated AgNPs induced
stronger effects compared to polymeric AgNPs. Interestingly, proteincoated AgNPs
reduced generation of reactive oxygen species in brains of females and
gonadectomised males. Although there were no significant differences in levels of
hormones in the AgNP-exposed animals compared to controls, sex-related differences
in oxidative stress parameters were observed in all organs. Results of this study
highlight the importance of including the sex-related differences and effects of
protein corona in biosafety evaluation of AgNPs exposure.
AN - WOS:000693220800003
AU - Lovakovic, B. T.
AU - Barbir, R.
AU - Pem, B.
AU - Goessler, W.
AU - Curlin, M.
AU - Micek, V.
AU - Debeljak, Z.
AU - Bozicevic, L.
AU - Ilic, K.
AU - Pavicic, I.
AU - Gorup, D.
AU - Vrcek, I. V.
C6 - JUL 2021
C7 - 100340
DA - JUL
DO - 10.1016/j.impact.2021.100340
PY - 2021
SN - 2452-0748
ST - Sex-related response in mice after sub-acute intraperitoneal exposure to
T2 - NANOIMPACT
TI - Sex-related response in mice after sub-acute intraperitoneal exposure to
VL - 23
ID - 6169
ER -
TY - JOUR
AB - Background: As a wound dressing and barrier membrane, surface modification of
polycaprolactone (PCL) is needed in order to achieve better biological activities.
Exosomes derived from mesenchymal stem cells (MSCs) hold significant tissue
regeneration promise. Silver nanoparticles (Ag) have been suggested as the surface
modification technique for various medical devices. Materials and Methods: Ag and
human bone marrow MSC (hBMSC)-derived exosomes (MSCs-exo) were used to modify the
PCL scaffold. The impact of different scaffolds on immune cells and MSC
proliferation and differentiation was further evaluated. Results: MSCs-exo
exhibited cup-shaped morphology with a diameter around 100 nm. MSCs-exo were
enriched with exosome marker CD81 and showed good internalization into recipient
cells. 200 ng/ml Ag nanoparticles and MSCs-exo were further used to modify the PCL
scaffold. The internalization study further indicated a similar releasing pattern
of exosomes from Ag/MSCs-exo hybrid scaffolds into RAW264.7 and hBMSCs at 12 and 24
h, respectively. Macrophages play an important role during different stages of bone
regeneration. The MTT and confocal microscopy study demonstrated no significant
toxicity of exosome and/or Ag hybrid scaffolds for macrophages and MSCs.
Inflammatory macrophages were further used to mimic the inflammatory environment. A
mixed population of elongated and round morphology was noted in the exosome and Ag
hybrid group, in which the proinflammatory genes and secretion of IL-6 and TNF-
alpha were significantly reduced. In addition, the exosome and Ag hybrid scaffolds
could significantly boost the osteogenic differentiation of hBMSCs. Discussion:
This study highlights the possibility of using Ag nanoparticles and MSCs-exo to
modify the PCL scaffold, thus providing new insight into the development of the
novel immunomodulatory biomembrane.
AN - WOS:000687384100001
AU - Lu, H. P.
AU - Zhang, Y.
AU - Xiong, S.
AU - Zhou, Y. H.
AU - Xiao, L.
AU - Ma, Y. P.
AU - Xiao, Y.
AU - Wang, X.
C7 - 699802
DA - AUG 2
DO - 10.3389/fchem.2021.699802
PY - 2021
SN - 2296-2646
ST - Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell-Derived
Exosome-Modified Barrier Membrane on Macrophages and Osteogenesis
T2 - FRONTIERS IN CHEMISTRY
TI - Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell-Derived
VL - 9
ID - 6085
ER -
TY - JOUR
AB - Background: As a wound dressing and barrier membrane, surface modification of
polycaprolactone (PCL) is needed in order to achieve better biological activities.
Exosomes derived from mesenchymal stem cells (MSCs) hold significant tissue
regeneration promise. Silver nanoparticles (Ag) have been suggested as the surface
modification technique for various medical devices. Materials and Methods: Ag and
human bone marrow MSC (hBMSC)-derived exosomes (MSCs-exo) were used to modify the
PCL scaffold. The impact of different scaffolds on immune cells and MSC
proliferation and differentiation was further evaluated. Results: MSCs-exo
exhibited cup-shaped morphology with a diameter around 100 nm. MSCs-exo were
enriched with exosome marker CD81 and showed good internalization into recipient
cells. 200 ng/ml Ag nanoparticles and MSCs-exo were further used to modify the PCL
scaffold. The internalization study further indicated a similar releasing pattern
of exosomes from Ag/MSCs-exo hybrid scaffolds into RAW264.7 and hBMSCs at 12 and
24 h, respectively. Macrophages play an important role during different stages of
bone regeneration. The MTT and confocal microscopy study demonstrated no
significant toxicity of exosome and/or Ag hybrid scaffolds for macrophages and
MSCs. Inflammatory macrophages were further used to mimic the inflammatory
environment. A mixed population of elongated and round morphology was noted in the
exosome and Ag hybrid group, in which the proinflammatory genes and secretion of
IL-6 and TNF-α were significantly reduced. In addition, the exosome and Ag hybrid
scaffolds could significantly boost the osteogenic differentiation of hBMSCs.
Discussion: This study highlights the possibility of using Ag nanoparticles and
MSCs-exo to modify the PCL scaffold, thus providing new insight into the
development of the novel immunomodulatory biomembrane. © Copyright © 2021 Lu,
Zhang, Xiong, Zhou, Xiao, Ma, Xiao and Wang.
AU - Lu, H.
AU - Zhang, Y.
AU - Xiong, S.
AU - Zhou, Y.
AU - Xiao, L.
AU - Ma, Y.
AU - Xiao, Y.
AU - Wang, X.
C7 - 699802
DB - Scopus
DO - 10.3389/fchem.2021.699802
KW - Ag
exosome
mesenchymal stem cells
osteogenesis
PCL hybrid scaffold
M3 - Article
PY - 2021
ST - Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived
TI - Modulatory Role of Silver Nanoparticles and Mesenchymal Stem Cell–Derived
85112689795&doi=10.3389%2ffchem.2021.699802&partnerID=40&md5=616dc82c69a26b2db27c9f
20cc1be111
VL - 9
ID - 5196
ER -
TY - JOUR
AB - Background: Sepsis is a syndrome of physiological, pathological and
biochemical abnormalities caused by infection. Although the mortality rate is lower
than before, many survivors have persistent infection, which means sepsis calls for
new treatment. After infection, inflammatory mediators were largely released into
the blood, leading to multiple organ dysfunction. Therefore, anti-infection and
anti-inflammation are critical issues in sepsis management. Results: Here, we
successfully constructed a novel nanometer drug loading system for sepsis
management, FZ/MER-AgMOF@Bm. The nanoparticles were modified with LPS-treated bone
marrow mesenchymal stem cell (BMSC) membrane, and silver metal organic framework
(AgMOF) was used as the nanocore for loading FPS-ZM1 and meropenem which was
delivery to the infectious microenvironments (IMEs) to exert dual anti-inflammatory
and antibacterial effects. FZ/MER-AgMOF@Bm effectively alleviated excessive
inflammatory response and eliminated bacteria. FZ/MER-AgMOF@Bm also played an anti-
inflammatory role by promoting the polarization of macrophages to M2. When sepsis
induced by cecal ligation and puncture (CLP) challenged mice was treated, FZ/MER-
AgMOF@Bm could not only reduce the levels of pro-inflammatory factors and lung
injury, but also help to improve hypothermia caused by septic shock and prolong
survival time. Conclusions: Together, the nanoparticles played a role in combined
anti-inflammatory and antimicrobial properties, alleviating cytokine storm and
protecting vital organ functions, could be a potential new strategy for sepsis
management. © 2023, The Author(s).
AU - Lu, L.
AU - Quan, L.
AU - Li, J.
AU - Yuan, J.
AU - Nie, X.
AU - Huang, X.
AU - Dong, H.
AU - Su, Y.
AU - Huang, Y.
AU - Kou, Q.
AU - Liu, L.
AU - Liu, H.
AU - Zhou, X.
AU - Gui, R.
AU - Gu, L.
C7 - 170
DB - Scopus
DO - 10.1186/s12951-023-01913-3
IS - 1
KW - Antibacterial
Cytokine storm
Metal-organic framework
sepsis
Animals
Cell Membrane
Macrophages
Mice
Nanoparticles
Sepsis
Bone
Cell culture
Mammals
Metal nanoparticles
Microorganisms
Organometallics
Stem cells
lipopolysaccharide
meropenem
nanocarrier
silver
silver metal organic framework
unclassified drug
antiinfective agent
nanoparticle
Anti-inflammatory and antimicrobial properties
Antibacterials
Cytokines
Functionalized nanoparticles
Metalorganic frameworks (MOFs)
Mortality rate
Stem-cell
animal cell
animal experiment
animal model
animal tissue
Article
bacterium
biocompatibility
bioengineering
cecal ligation and puncture-induced sepsis
cell membrane
controlled study
cytokine storm
disease management
drug loading system
drug safety
hypothermia
in vitro study
in vivo study
infection
inflammation
lung injury
M2 macrophage
macrophage
microenvironment
mouse
nonhuman
polarization
septic shock
survival time
animal
disease model
metabolism
Storms
M3 - Article
PY - 2023
ST - Bioengineered stem cell membrane functionalized nanoparticles combine anti-
inflammatory and antimicrobial properties for sepsis treatment
TI - Bioengineered stem cell membrane functionalized nanoparticles combine anti-
inflammatory and antimicrobial properties for sepsis treatment
85160377269&doi=10.1186%2fs12951-023-01913-
3&partnerID=40&md5=08b59414091e30f35696125a4e73191f
VL - 21
ID - 4990
ER -
TY - JOUR
AB - Glycine-proline-glutamate (GPE) is an N-terminal tripeptide endogenously
cleaved from insulin-like growth factor-1 in the brain and is neuroprotective
against hypoxic-ischemic brain injury and neurodegeneration. NNZ-2566 is an analog
of GPE designed to have improved bioavailability. In this study, we tested NNZ-2566
in a rat model of penetrating ballistic-type brain injury (PBBI) and assessed its
effects on injury-induced histopathology, behavioral deficits, and molecular and
cellular events associated with inflammation and apoptosis. In the initial dose-
response experiments, NNZ-2566 (0.01-3 mg/kg/h x 12 h intravenous infusion) was
given at 30 min post-injury and the therapeutic time window was established by
delaying treatments 2-4 h post-injury, but with the addition of a 10- or 30-mg/kg
bolus dose. All animals survived 72 h. Neuroprotection was evaluated by balance
beam testing and histopathology. The effects of NNZ-2566 on injury-induced changes
in Bax and Bcl-2 proteins, activated microgliosis, neutrophil infiltration, and
astrocyte reactivity were also examined. Behavioral results demonstrated that NNZ-
2566 dose-dependently reduced foot faults by 19-66% after acute treatments, and 35-
55% after delayed treatments. Although gross lesion volume was not affected, NNZ-
2566 treatment significantly attenuated neutrophil infiltration and reduced the
number of activated microglial cells in the peri-lesion regions of the PBBI. PBBI
induced a significant upregulation in Bax expression (36%) and a concomitant
downregulation in Bcl-2 expression (33%), both of which were significantly reversed
by NNZ-2566. Collectively, these results demonstrated that NNZ-2566 treatment
promoted functional recovery following PBBI, an effect related to the modulation of
injury-induced neural inflammatory and apoptotic mechanisms.
AN - WOS:000263516700012
AU - Lu, X. C. M.
AU - Chen, R. W.
AU - Yao, C. P.
AU - Wei, H.
AU - Yang, X. F.
AU - Liao, Z. L.
AU - Dave, J. R.
AU - Tortella, F. C.
DA - JAN
DO - 10.1089/neu.2008.0629
IS - 1
PY - 2009
SN - 0897-7151
1557-9042
SP - 141-154
ST - NNZ-2566, a Glypromate Analog, Improves Functional Recovery and Attenuates
Apoptosis and Inflammation in a Rat Model of Penetrating Ballistic-Type Brain
Injury
T2 - JOURNAL OF NEUROTRAUMA
TI - NNZ-2566, a Glypromate Analog, Improves Functional Recovery and Attenuates
Apoptosis and Inflammation in a Rat Model of Penetrating Ballistic-Type Brain
Injury
VL - 26
ID - 6554
ER -
TY - JOUR
AB - In recent years, the immune-potentiating effects of some widely used
chemotherapeutic agents have been increasingly appreciated. This provides a
rationale for combining conventional chemotherapy with immunotherapy strategies to
achieve durable therapeutic benefits. Previous studies have implicated the
immunomodulatory effects of melphalan, an alkylating agent commonly used to treat
multiple myeloma, but the underlying mechanisms remain obscure. In the present
study, we investigated the impact of melphalan on endogenous immune cells as well
as adoptively transferred tumor-specific CD4+ T cells in tumor-bearing mice. We
showed that melphalan treatment resulted in a rapid burst of inflammatory cytokines
and chemokines during the cellular recovery phase after melphalan-induced
myelodepletion and leukodepletion. After melphalan treatment, tumor cells exhibited
characteristics of immunogenic cell death, including membrane translocation of the
endoplasmic reticulum-resident calreticulin and extracellular release of high-
mobility group box 1. Additionally, there was enhanced tumor Ag uptake by dendritic
cells in the tumor-draining lymph node. Consistent with these immunomodulatory
effects, melphalan treatment of tumor-bearing mice led to the activation of the
endogenous CD8+ T cells and, more importantly, effectively drove the clonal
expansion and effector differentiation of adoptively transferred tumor-specific
CD4+ T cells. Notably, the combination of melphalan and CD4+ T cell adoptive cell
therapy was more efficacious than either treatment alone in prolonging the survival
of mice with advanced B cell lymphomas or colorectal tumors. These findings provide
mechanistic insights into melphalan's immunostimulatory effects and demonstrate the
therapeutic potential of combining melphalan with adoptive cell therapy utilizing
antitumor CD4+ T cells. Copyright © 2015 by The American Association of
Immunologists, Inc.
AU - Lu, X.
AU - Ding, Z. C.
AU - Cao, Y.
AU - Liu, C.
AU - Habtetsion, T.
AU - Yu, M.
AU - Lemos, H.
AU - Salman, H.
AU - Xu, H.
AU - Mellor, A. L.
AU - Zhou, G.
DB - Scopus
DO - 10.4049/jimmunol.1401894
IS - 4
KW - Animals
Antineoplastic Agents, Alkylating
Blotting, Western
CD4-Positive T-Lymphocytes
Combined Modality Therapy
Flow Cytometry
Immunotherapy, Adoptive
Melphalan
Mice
Mice, Inbred BALB C
Mice, Transgenic
Neoplasms, Experimental
calreticulin
CXCL11 chemokine
gamma interferon
interleukin 10
interleukin 18
interleukin 1beta
interleukin 22
interleukin 27
interleukin 5
melphalan
silver
alkylating agent
adoptive immunotherapy
adoptive transfer
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
B cell lymphoma
bone marrow depression
CD4+ T lymphocyte
CD8+ T lymphocyte
cell expansion
clonal variation
colorectal carcinoma
controlled study
cytokine release
dendritic cell
drug mechanism
immunogenicity
immunomodulation
leukopenia
mouse
nonhuman
animal
Bagg albino mouse
flow cytometry
multimodality cancer therapy
procedures
transgenic mouse
transplantation
Western blotting
M3 - Article
PY - 2015
SP - 2011-2021
ST - Alkylating agent melphalan augments the efficacy of adoptive immunotherapy
using tumor-specific CD4+ T cells
T2 - Journal of Immunology
TI - Alkylating agent melphalan augments the efficacy of adoptive immunotherapy
using tumor-specific CD4+ T cells
84922552740&doi=10.4049%2fjimmunol.1401894&partnerID=40&md5=9f293dc305ce793e08fc132
b0b2044fe
VL - 194
ID - 5597
ER -
TY - JOUR
AB - AA amyloidosis is a systemic disease that develops secondary to chronic
inflammatory diseases Macrophages are often found in the vicinity of amyloid
deposits and considered to play a role in both formation and degradation of amyloid
fibrils. In spleen reside at least three types of macrophages, red pulp macrophages
(RPM), marginal zone macrophages (MZM), metallophilic marginal zone macrophages
(MMZM). MMZM and MZM are located in the marginal zone and express a unique
collection of scavenger receptors that are involved in the uptake of blood-born
particles. The murine AA amyloid model that resembles the human form of the disease
has been used to study amyloid effects on different macrophage populations. Amyloid
was induced by intravenous injection of amyloid enhancing factor and subcutaneous
injections of silver nitrate and macrophages were identified with specific
antibodies. We show that MZMs are highly sensitive to amyloid and decrease in
number progressively with increasing amyloid load. Total area of MMZMs is
unaffected by amyloid but cells are activated and migrate into the white pulp. In a
group of mice spleen macrophages were depleted by an intravenous injection of
clodronate filled liposomes. Subsequent injections of AEF and silver nitrate showed
a sustained amyloid development. RPMs that constitute the majority of macrophages
in spleen, appear insensitive to amyloid and do not participate in amyloid
formation. © 2013 Lundmark et al.
AU - Lundmark, K.
AU - Shariatpanahi, A. V.
AU - Westermark, G. T.
C7 - e79104
DB - Scopus
DO - 10.1371/journal.pone.0079104
IS - 11
KW - Amyloidosis
Animals
Animals, Outbred Strains
Female
Humans
Macrophages
Mice
Serum Amyloid A Protein
Spleen
amyloid enhancing factor
clodronic acid
enzyme
liposome
silver nitrate
unclassified drug
amyloidosis
animal cell
animal experiment
animal model
animal tissue
article
cell population
controlled study
cytotoxicity
drug mechanism
female
macrophage
macrophage function
marginal zone macrophage
metallophilic marginal zone macrophage
mouse
nonhuman
red pulp macrophage
regulatory mechanism
sensitivity analysis
spleen cell
M3 - Article
PY - 2013
ST - Depletion of spleen macrophages delays AA amyloid development: A study
performed in the rapid mouse model of aa amyloidosis
T2 - PLoS ONE
TI - Depletion of spleen macrophages delays AA amyloid development: A study
performed in the rapid mouse model of aa amyloidosis
84893374758&doi=10.1371%2fjournal.pone.0079104&partnerID=40&md5=510bc74a9be9fad68c3
f534682fdf946
VL - 8
ID - 5639
ER -
TY - JOUR
AB - This study aimed to determine the chronic toxicity of the ionic liquid (IL)
1-methyl-3-octylimidazolium bromide ([C(8)mim]Br) on silver carp to further study
the toxicological mechanism of ILs. For this purpose, 60-d chronic exposure at
concentrations of 1.09 or 4.38 mg L-1 [C(8)mim]Br in silver carp was conducted. The
results of biochemical assays revealed that [C(8)mim]Br-treatment remarkably
promoted serum lactate dehydrogenase (LDH), aspartate aminotransferase (AST),
alanine aminotransferase (ALT), acid phosphatase (ACP), and alkaline phosphatase
(AKP) activities, indicating that [C(8)mim]Br-exposure caused fish organ damage.
Long-term exposure of [C(8)mim]Br also altered the activities of superoxide
dismutase (SOD) and catalase (CAT) and the glutathione (GSH) level but increased
malondialdehyde (MDA) levels in fish brain, gill, intestine, kidney, liver, and
muscle, suggesting that [C(8)mim]Br-treatment may cause oxidative stress in fish
organs. Further work revealed that [C(8)mim]Br-treatment increased the activities
of erythromycin-N-demethylase (ERND) and glutathione S-transferases (GST), which
may participate in the metabolism of [C(8)mim]Br in fish liver. Moreover, chronic
[Csmim]Br-exposure remarkably promoted the expression of inducible nitric oxide
synthase (iNOS), interleukin-10 (IL-10), tumour necrosis factor-alpha (TNF-alpha),
and nuclear factor-KB (NF-kappa B) and altered the levels of transforming growth
factor-beta (TGF-beta), suggesting that long-term exposure of [C(8)mim]Br might
promote the inflammatory response in fish liver. Additionally, [Camim]Br-exposure
altered succinate dehydrogenase (SDH) activity and promoted caspase-9 and caspase-3
activities in fish liver, suggesting that chronic [C(8)mim]Brexposure also induces
hepatocellular apoptosis via the mitochondrial pathway. The results presented here
may be helpful to illuminate the chronic toxicity mechanism of imidazolium-based
ILs and safe use of ILs in the future. (C) 2018 Elsevier Ltd. All rights reserved.
AN - WOS:000448493700039
AU - Ma, J. G.
AU - Li, X. X.
AU - Cui, M. K.
AU - Li, W. G.
AU - Li, X. Y.
DA - DEC
DO - 10.1016/j.chemosphere.2018.09.075
PY - 2018
SN - 0045-6535
1879-1298
SP - 358-367
ST - Negative impact of the imidazolium-based ionic liquid [C(8)mim]Br on silver
carp (Hypophthalmichthys molitrix): Long-term and low-level exposure
T2 - CHEMOSPHERE
TI - Negative impact of the imidazolium-based ionic liquid [C(8)mim]Br on silver
VL - 213
ID - 6434
ER -
TY - JOUR
AB - The present study aimed to determine the chronic toxicity of 1-methyl-3-
octylimidazolium bromide ([C8mim]Br) on the silver carp to further reveal the
toxicological mechanisms of ionic liquids. Chronic exposure of silver carp to
[C8mim]Br at concentrations of 1.095 and 4.380 mg/L for 60 d was conducted under
laboratory conditions. The results revealed that chronic exposure to [C8mim]Br
inhibited the activity of superoxide dismutase (SOD), catalase (CAT), and
glutathione peroxidase (GPx) and reduced glutathione (GSH) levels while markedly
increasing malondialdehyde (MDA) and protein carbonyl (PC) levels in fish spleen,
indicating that [C8mim]Br treatment induced oxidative stress. Additionally, long-
term exposure to [C8mim]Br markedly upregulated the expressions of nuclear factor-
κB (NF-κB), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), IL-6,
tumour necrosis factor-α (TNF-α), and interferon-γ (IFN-γ); altered the levels of
transforming growth factor-β (TGF-β); and increased the mRNA levels of p38MAPK, c-
fos, c-jun, and c-myc, suggesting that long-term exposure to [C8mim]Br might
promote the inflammatory response in fish spleen and that p38MAPK/NF-κB signalling
may potentially be involved in this process. Moreover, [C8mim]Br-exposure altered
lysozyme activity and complement 3 (C3) and immunoglobulin M (IgM) content,
indicating that chronic [C8mim]Br exposure also has immunotoxic effects on silver
carp. Furthermore, we also found that [C8mim]Br exposure reduced miR-125b levels,
altered miR-143 levels, and upregulated miR-155 and miR-21 levels, suggesting that
these miRNAs may be involved in the [C8mim]Br-induced inflammatory response in fish
spleen. In summary, the present study indicates that chronic exposure to [C8mim]Br
induces inflammation in fish spleen and that oxidative stress-mediated p38MAPK/NF-
κB signalling and miRNAs may play a key role in this process. © 2018 Elsevier Ltd
AU - Ma, J.
AU - Chen, X.
AU - Xin, G.
AU - Li, X.
DB - Scopus
DO - 10.1016/j.fsi.2018.09.052
KW - Inflammatory response
Ionic liquids
microRNAs
Oxidative stress
p38MAPK/NF-κB
Silver carp
Animals
Carps
Dose-Response Relationship, Immunologic
Fish Diseases
Fish Proteins
Imidazoles
Inflammation
Ionic Liquids
MicroRNAs
NF-kappa B
p38 Mitogen-Activated Protein Kinases
Signal Transduction
Spleen
Toxicity Tests, Chronic
1 methyl 3 octylimidazolium bromide
bromine
catalase
gamma interferon
glutathione
immunoglobulin M
interleukin 1beta
interleukin 6
ionic liquid
lysozyme
malonaldehyde
messenger RNA
microRNA
microRNA 143
microRNA 155
microRNA 21
Myc protein
protein c fos
protein c jun
unclassified drug
1-methyl-3-octylimidazolium bromide
fish protein
animal cell
animal experiment
Article
carp
controlled study
immunotoxicity
inflammation
long term exposure
nonhuman
oxidative stress
priority journal
protein expression
signal transduction
spleen
upregulation
animal
chemically induced
dose response
drug effect
fish disease
genetics
immunology
metabolism
physiology
toxicity
toxicity testing
veterinary medicine
water pollutant
M3 - Article
PY - 2019
SP - 627-638
ST - Chronic exposure to the ionic liquid [C8mim]Br induces inflammation in silver
carp spleen: Involvement of oxidative stress-mediated p38MAPK/NF-κB signalling and
microRNAs
T2 - Fish and Shellfish Immunology
TI - Chronic exposure to the ionic liquid [C8mim]Br induces inflammation in silver
carp spleen: Involvement of oxidative stress-mediated p38MAPK/NF-κB signalling and
microRNAs
85055525688&doi=10.1016%2fj.fsi.2018.09.052&partnerID=40&md5=e7629398a9d884d18255c0
2fc1e5feca
VL - 84
ID - 5411
ER -
TY - JOUR
AB - This study aimed to determine the chronic toxicity of the ionic liquid (IL)
1-methyl-3-octylimidazolium bromide ([C8mim]Br) on silver carp to further study the
toxicological mechanism of ILs. For this purpose, 60-d chronic exposure at
concentrations of 1.09 or 4.38 mg L−1 [C8mim]Br in silver carp was conducted. The
results of biochemical assays revealed that [C8mim]Br-treatment remarkably promoted
serum lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine
aminotransferase (ALT), acid phosphatase (ACP), and alkaline phosphatase (AKP)
activities, indicating that [C8mim]Br-exposure caused fish organ damage. Long-term
exposure of [C8mim]Br also altered the activities of superoxide dismutase (SOD) and
catalase (CAT) and the glutathione (GSH) level but increased malondialdehyde (MDA)
levels in fish brain, gill, intestine, kidney, liver, and muscle, suggesting that
[C8mim]Br-treatment may cause oxidative stress in fish organs. Further work
revealed that [C8mim]Br-treatment increased the activities of erythromycin-N-
demethylase (ERND) and glutathione S-transferases (GST), which may participate in
the metabolism of [C8mim]Br in fish liver. Moreover, chronic [C8mim]Br-exposure
remarkably promoted the expression of inducible nitric oxide synthase (iNOS),
interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), and nuclear factor-κB
(NF-κB) and altered the levels of transforming growth factor-β (TGF-β), suggesting
that long-term exposure of [C8mim]Br might promote the inflammatory response in
fish liver. Additionally, [C8mim]Br-exposure altered succinate dehydrogenase (SDH)
activity and promoted caspase-9 and caspase-3 activities in fish liver, suggesting
that chronic [C8mim]Br-exposure also induces hepatocellular apoptosis via the
mitochondrial pathway. The results presented here may be helpful to illuminate the
chronic toxicity mechanism of imidazolium-based ILs and safe use of ILs in the
future. © 2018
AU - Ma, J.
AU - Li, X.
AU - Cui, M.
AU - Li, W.
DB - Scopus
DO - 10.1016/j.chemosphere.2018.09.075
KW - Chronic toxicity
Freshwater fish
Ionic liquid
Toxic mechanism
Animals
Carps
Fishes
Imidazoles
Ionic Liquids
Hypophthalmichthys molitrix
Hypophthalmichthys nobilis
Alkalinity
Amino acids
Brain
Cell death
Ionic liquids
Nitric oxide
Peptides
Phosphatases
Plants (botany)
Silver
Toxicity
1 methyl 3 octylimidazolium bromide
acid phosphatase
caspase 3
caspase 9
catalase
erythromycin n demethylase
glutathione
interleukin 1beta
ionic liquid
malonaldehyde
n demethylase
succinate dehydrogenase
unclassified drug
imidazole
1-methyl-3-octylimidazolium bromide
Aspartate aminotransferase
Freshwater fishes
Imidazolium-based ionic liquid
Inducible nitric oxide synthase (iNOS)
Transforming growth factor beta
Tumour necrosis factor alphas
apoptosis
cyprinid
enzyme activity
fish
freshwater environment
growth
metabolism
nitric oxide
oxidative stress
phosphatase
pollution effect
pollution exposure
protein
serum
toxicity
toxicology
acid phosphatase blood level
animal cell
animal experiment
animal tissue
Article
brain
carp
chronic toxicity
comparative study
controlled study
enzyme induction
gill
intestine
juvenile animal
kidney
lipid peroxidation
liver
liver cell
liver microsome
long term exposure
muscle
nonhuman
protein expression
animal
chemistry
Fish
M3 - Article
PY - 2018
SP - 358-367
ST - Negative impact of the imidazolium-based ionic liquid [C8mim]Br on silver
T2 - Chemosphere
TI - Negative impact of the imidazolium-based ionic liquid [C8mim]Br on silver
85054640388&doi=10.1016%2fj.chemosphere.2018.09.075&partnerID=40&md5=bacc359484df5f
f5c5b36195b567d97e
VL - 213
ID - 5380
ER -
TY - JOUR
AB - Mast cells (MCs) play critical roles in allergic reactions and modulating the
activation of MCs could be an effective strategy to treat allergic diseases, which
cause a rapidly increasing threat to the public health. Herein, we described that
Magnolin, a major component from Flos magnoliae could inhibit IgE-dependent MCs
activation. We found Magnolin inhibited IgE/Ag-induced calcium mobilization,
degranulation, and cytokines release in LAD2 cells. Magnolin was also found to
attenuate IgE/Ag-induced mice paw swelling in a dose-dependent manner. Further
mechanistic studies suggested a possible anti-allergic and anti-inflammatory
effects of Magnolin in IgE/Ag-induced anaphylactic reactions. Thereby, Magnolin
could be a potential therapeutic agent for preventing mast cell-related immediate
and delayed allergic diseases. © 2019 Elsevier B.V.
AU - Ma, P.
AU - Che, D.
AU - Zhao, T.
AU - Zhang, Y.
AU - Li, C.
AU - An, H.
AU - Zhang, T.
AU - He, H.
C7 - 105867
DB - Scopus
DO - 10.1016/j.intimp.2019.105867
KW - Anti-anaphylactic
Calcium activation
Degranulation
Magnolin
Mast cells
Anaphylaxis
Animals
Anti-Allergic Agents
Antigens
Calcium
Cell Line
Cell Survival
Cytokines
Edema
Histamine
Humans
Hypersensitivity
Immunoglobulin E
Lignans
Male
Mast Cells
Mice, Inbred BALB C
Mitogen-Activated Protein Kinases
Peptide Hydrolases
antiallergic agent
immunoglobulin E
magnolin
silver
unclassified drug
antigen
calcium
cytokine
histamine
lignan
peptide hydrolase
adult
allergy
anaphylaxis
animal experiment
animal model
Article
calcium mobilization
cytokine release
cytotoxicity
degranulation
downstream processing
in vitro study
in vivo study
LAD 2 cell line
male
mouse
nonhuman
paw edema
priority journal
signal transduction
animal
Bagg albino mouse
cell line
cell survival
drug effect
edema
human
hypersensitivity
immunology
mast cell
metabolism
M3 - Article
PY - 2019
ST - Magnolin inhibits IgE/Ag-induced allergy in vivo and in vitro
TI - Magnolin inhibits IgE/Ag-induced allergy in vivo and in vitro
85071974644&doi=10.1016%2fj.intimp.2019.105867&partnerID=40&md5=955207d76bae2678a3d
73784187f98ad
VL - 76
ID - 5372
ER -
TY - JOUR
AB - In this study, different phenolic extracts were obtained from the jaboticaba
skin meal (JSM), whose phenolic compounds were characterized and their
antibacterial activities were assessed. Moreover, the activity of lyophilized
ethanolic extract of jaboticaba skin (EEJS) on wound healing was analyzed in rats.
The JSM phenolic extracts were obtained in four ways: aqueous, methanolic,
ethanolic, and acetone extracts. The phenolic compounds were characterized in these
extracts by high-performance liquid chromatography, and their antibacterial
activities were evaluated. The in vivo experiment was divided into four groups and
received the following treatments: G1—silver sulfadiazine (positive control); G2—
EEJS at 10%; G3—EEJS at 5%, and G4—EEJS at 2.5%. The aqueous extract did not
inhibit the growing of any bacterium. The ethanolic, acetone, and methanolic
extracts inhibited the growing of all bacteria tested at the concentrations of
1.25%, 2.50%, and 5.00%, respectively. The ethanolic extract was the one that
showed the highest bacterial inhibition potential and the highest contents of
phenolic compounds, especially of catechin, epicatechin gallate, and epicatechin.
The G3 and G4 treatments presented faster wound healing compared to the G1 one, as
it promoted a less intense inflammatory reaction and full closure of the wounds at
an accelerated rate. © 2018 John Wiley & Sons A/S
AU - Machado, G. H. A.
AU - Marques, T. R.
AU - de Carvalho, T. C. L.
AU - Duarte, A. C.
AU - de Oliveira, F. C.
AU - Gonçalves, M. C.
AU - Piccoli, R. H.
AU - Corrêa, A. D.
DB - Scopus
DO - 10.1111/cbdd.13198
IS - 1
KW - antimicrobial
HPLC
Plinia jaboticaba
wound healing
Animals
Anthocyanins
Chromatography, High Pressure Liquid
Fruit
Male
Myrtaceae
Phenols
Plant Extracts
Rats
Rats, Wistar
Skin
Wound Healing
acetone
alcohol
catechin
coumaric acid
cyanidin chloride
delphinidin
epicatechin
epicatechin gallate
ferulic acid
flavonoid
gallic acid
jaboticaba skin meal extract
malvidin chloride
methanol
para coumaric acid
phenol derivative
plant extract
sulfadimethoxine
syringic acid
tannin derivative
unclassified drug
anthocyanin
antiinfective agent
animal cell
animal experiment
animal model
animal tissue
Article
bioassay
chromatography
cytotoxicity test
erythrocyte membrane
flow rate
fruit
hemolysis
histology
in vitro study
in vivo study
nonhuman
priority journal
rat
retention time
secondary infection
skin injury
Wistar rat
wound closure
animal
chemistry
drug effect
male
metabolism
pathology
skin
M3 - Article
PY - 2018
SP - 1333-1343
ST - Antibacterial activity and in vivo wound healing potential of phenolic
extracts from jaboticaba skin
T2 - Chemical Biology and Drug Design
TI - Antibacterial activity and in vivo wound healing potential of phenolic
extracts from jaboticaba skin
85049054431&doi=10.1111%2fcbdd.13198&partnerID=40&md5=c444c1d4ffcc36a88cc4768350106
1ac
VL - 92
ID - 5542
ER -
TY - JOUR
AB - The highlights of biogenic silver nanoparticles (AgNp-Bio) include low
toxicity - depending on size and concentration - and efficient antiparasitic
activity. Therefore, the objective of this study was to assess the action of the
AgNp-Bio on HeLa cells in an infection with strain of RH Toxoplasma gondii.
Firstly, we performed a cellular viability test and characterized the AgNp-Bio to
proceed with the infection of HeLa cells with T. gondii to be treated using AgNp-
Bio or conventional drugs. Subsequently, we determined the level of standard
cytokines Th1/Th2 as well as the content of nitric oxide (NO) and reactive oxygen
species (ROS). Results indicated a mean size of 69 nm in diameter for the AgNp-Bio
and obtained a dose-dependent toxicity. In addition, the concentrations of 3 and 6
pM promoted a significant decrease in adherence, infection, and intracellular
proliferation. We also found lower IL-8 and production of inflammatory mediators.
Thus, the nanoparticles reduced the adherence, infection, and proliferation of ROS
and NO, in addition to immunomodulating the IL-8. Therefore, our data proved
relevant to introduce a promising therapeutic alternative to toxoplasmosis.
AN - WOS:000522799000015
AU - Machado, L. F.
AU - Sanfelice, R. A.
AU - Bosqui, L. R.
AU - Assolini, J. P.
AU - Navarro, I. T.
AU - Cataneo, A. H. D.
AU - Wowk, P. F.
AU - Nakazato, G.
AU - Bordignon, J.
AU - Costa, I. N.
C7 - 107853
DA - APR
DO - 10.1016/j.exppara.2020.107853
PY - 2020
SN - 0014-4894
1090-2449
ST - Biogenic silver nanoparticles reduce adherence, infection, and proliferation
of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators
induction
T2 - EXPERIMENTAL PARASITOLOGY
TI - Biogenic silver nanoparticles reduce adherence, infection, and proliferation
induction
VL - 211
ID - 5906
ER -
TY - JOUR
AB - It is a research paper that highlights the importance of alternative
treatment with AgNp-Bio in human toxoplasmosis in order to minimize the damaging
effects caused by conventional drugs (sulfadiazine and pyrimethamine) used today. ©
2020; The highlights of biogenic silver nanoparticles (AgNp-Bio) include low
toxicity – depending on size and concentration – and efficient antiparasitic
activity. Therefore, the objective of this study was to assess the action of the
AgNp-Bio on HeLa cells in an infection with strain of RH Toxoplasma gondii.
Firstly, we performed a cellular viability test and characterized the AgNp-Bio to
proceed with the infection of HeLa cells with T. gondii to be treated using AgNp-
Bio or conventional drugs. Subsequently, we determined the level of standard
cytokines Th1/Th2 as well as the content of nitric oxide (NO) and reactive oxygen
species (ROS). Results indicated a mean size of 69 nm in diameter for the AgNp-Bio
and obtained a dose-dependent toxicity. In addition, the concentrations of 3 and 6
μM promoted a significant decrease in adherence, infection, and intracellular
proliferation. We also found lower IL-8 and production of inflammatory mediators.
Thus, the nanoparticles reduced the adherence, infection, and proliferation of ROS
and NO, in addition to immunomodulating the IL-8. Therefore, our data proved
relevant to introduce a promising therapeutic alternative to toxoplasmosis. © 2020
AU - Machado, L. F.
AU - Sanfelice, R. A.
AU - Bosqui, L. R.
AU - Assolini, J. P.
AU - Navarro, I. T.
AU - Depieri Cataneo, A. H.
AU - Wowk, P. F.
AU - Nakazato, G.
AU - Bordignon, J.
AU - Costa, I. N.
C7 - 107853
DB - Scopus
DO - 10.1016/j.exppara.2020.107853
KW - HeLa cells
Toxoplasmosis
Treatment
biogenic silver nanoparticle
interleukin 8
nitric oxide
silver nanoparticle
unclassified drug
Article
cell adhesion
cell proliferation
controlled study
cytokine production
HeLa cell line
immunomodulation
nonhuman
particle size
priority journal
Th1 cell
Th2 cell
Toxoplasma gondii
toxoplasmosis
M3 - Article
PY - 2020
ST - Biogenic silver nanoparticles reduce adherence, infection, and proliferation
induction
T2 - Experimental Parasitology
TI - Biogenic silver nanoparticles reduce adherence, infection, and proliferation
induction
85079383412&doi=10.1016%2fj.exppara.2020.107853&partnerID=40&md5=dbfdc90b6205eb6acc
f8a984fd8e1953
VL - 211
ID - 5329
ER -
TY - JOUR
AB - This study aimed to evaluate the Carbon Fiber obtained from PAN textile and
cotton fiber in their different forms of presentation: non-activated carbon fiber
felt (NACFF), activated carbon fiber felt (ACFF), silver activated carbon fiber
felt (Ag-ACFF), and activated carbon fiber tissue (ACFT), to obtain scaffolds as a
potential material with properties related to the synthetic bone graft.
Characterization tests performed: surface wettability, traction, swelling, and in
vivo tests: evaluation of the inflammatory response by implanting the materials in
the subcutaneous tissue of 14 Wistar rats, evaluation of collagen fibers by
picrosirius red staining and assessment of toxicity in the following organs: heart,
spleen, liver, and kidney. In the wettability test, NACFF and ACFT were hydrophobic
(θ124° and 114°), ACFF and Ag-ACFF were hydrophilic. For maximum stress, ACFF was
more resistant (2.983 ± 1.059) p <.05. In the swelling test, the Ag-ACFF and ACFF
groups showed the highest absorption percentage for the PBS solution and distilled
water (p <.001). The organs showed no signs of acute systemic toxicity. The implant
regions showed mild to moderate inflammatory infiltrate at 7 and 21 days. Only the
ACFT group did not show the maturation of type I collagen fibers in 21 days.
Through the conducted analyses, the ACFT shows little potential to be indicated as
a possible scaffold. Therefore NACFF, ACFF, and Ag-ACFF have the potential to be
considered scaffolds due to the following characteristics presented: good
absorption rate, hydrophilicity, and non-toxic. © 2023 Wiley Periodicals LLC.
AU - Maciel, C. C. M.
AU - Torquato, L. C.
AU - Chelin Suárez, E. A.
AU - Pereira, K. A.
AU - Jardini, M. A. N.
AU - Borges, A. L. S.
AU - de Vasconcellos, L. M. R.
AU - Marcuzzo, J. S.
AU - De Marco, A. C.
DB - Scopus
DO - 10.1002/jbm.b.35298
KW - biocompatible materials
bone regeneration
carbon fiber
scaffolds
tissue engineering
Biocompatibility
Collagen
Felt
Hydrophilicity
Rats
Scaffolds (biology)
Stem cells
Swelling
Tissue
Tissue regeneration
Toxicity
Water absorption
Wetting
Activated carbon fibres
Bone regeneration
Carbon fiber felts
Collagen fibres
Fiber characterizations
Fiber tissue
Potential materials
Property
Carbon fibers
M3 - Article
PY - 2023
ST - Carbon fiber: Characterization and evaluation of the inflammatory response
and toxicity in rats
T2 - Journal of Biomedical Materials Research - Part B Applied Biomaterials
TI - Carbon fiber: Characterization and evaluation of the inflammatory response
85165508789&doi=10.1002%2fjbm.b.35298&partnerID=40&md5=942e225247084dcd4262abca735f
c53c
ID - 4978
ER -
TY - JOUR
AB - This study aimed to evaluate the Carbon Fiber obtained from PAN textile and
cotton fiber in their different forms of presentation: non-activated carbon fiber
felt (NACFF), activated carbon fiber felt (ACFF), silver activated carbon fiber
felt (Ag-ACFF), and activated carbon fiber tissue (ACFT), to obtain scaffolds as a
potential material with properties related to the synthetic bone graft.
Characterization tests performed: surface wettability, traction, swelling, and in
vivo tests: evaluation of the inflammatory response by implanting the materials in
the subcutaneous tissue of 14 Wistar rats, evaluation of collagen fibers by
picrosirius red staining and assessment of toxicity in the following organs: heart,
spleen, liver, and kidney. In the wettability test, NACFF and ACFT were hydrophobic
(theta 124 degrees and 114 degrees), ACFF and Ag-ACFF were hydrophilic. For maximum
stress, ACFF was more resistant (2.983 +/- 1.059) p <.05. In the swelling test, the
Ag-ACFF and ACFF groups showed the highest absorption percentage for the PBS
solution and distilled water (p <.001). The organs showed no signs of acute
systemic toxicity. The implant regions showed mild to moderate inflammatory
infiltrate at 7 and 21 days. Only the ACFT group did not show the maturation of
type I collagen fibers in 21 days. Through the conducted analyses, the ACFT shows
little potential to be indicated as a possible scaffold. Therefore NACFF, ACFF, and
Ag-ACFF have the potential to be considered scaffolds due to the following
characteristics presented: good absorption rate, hydrophilicity, and non-toxic.
AN - WOS:001034613700001
AU - Maciel, C. C. M.
AU - Torquato, L. C.
AU - Suarez, E. A. C.
AU - Pereira, K. A.
AU - Jardini, M. A. N.
AU - Borges, A. L. S.
AU - de Vasconcellos, L. M. R.
AU - Marcuzzo, J. S.
AU - De Marco, A. C.
C6 - JUL 2023
DA - 2023 JUL 22
DO - 10.1002/jbm.b.35298
PY - 2023
SN - 1552-4973
1552-4981
ST - Carbon fiber: Characterization and evaluation of the inflammatory response
TI - Carbon fiber: Characterization and evaluation of the inflammatory response
ID - 6289
ER -
TY - JOUR
AB - The widespread use of silver nanoparticles (AgNPs) would likely result in
their discharge into wastewater and inevitable release in densely populated coastal
areas. It is known that AgNPs can cause harmful effects to marine fauna, but how
they affect development stages is still an open question. In order to understand in
details how polymer-coated AgNPs (PAAm-AgNPs) (from 0.19 to 4.64 mM as Ag) can
affect critical stages of marine invertebrate development, metamorphic larvae and
juveniles of sea urchins were used as biological models. Multidimensional scaling
(MDS) approach based on Bray-Curtis similarity matrix with PERMANOVA showed
organisms in a multivariate space undergoing through different physiological
conditions as a function of time, chemical forms of silver, nominal concentrations,
and presence or absence of food. Sublethal effects such as lethargy, oedema and
immobility mainly characterized PAAm-AgNPs effects with juveniles and postlarvae,
whereas necrosis and death arose in Ag+ conditions in short-term tests. Chronically
exposed metamorphic larvae had their morphogenic processes interrupted by PAAm-
AgNPs and a high mortality rate was observed in recovery period. On the contrary,
Ag+ ions caused progressive mortality during exposure, but a quick recovery in
uncontaminated seawater was observed. By means of fluorescent markers we showed
that nanosilver could be transferred between consecutive stages (swimming larvae
and postlarvae) and highlighted how important is food to enhance PAAm-AgNPs uptake.
Using TEM we observed that unfed juveniles had nanosilver aggregates mostly
restricted to their coelomic sinuses, while metamorphic larvae already had nano-
contamination overspread in different tissues and blastocoel. Our main hypothesis
for nanotoxicity of PAAM-AgNPs relies on the slow dissolution of nano-core over
time, but in this study the effects of particulate silver form itself are also
evoked. Main mechanisms governing tissular and cellular responses to nano-
intoxication such as inflammatory response and detoxification based on the role of
sentinel cells (peritoneal cells and coelomocytes) for general homeostasis are
discussed. This paper is first to detail physiological states, main uptake routes
and cellular response against polymer-coated AgNPs in developmental stages of
marine invertebrate species. © 2016 Elsevier B.V..
AU - Magesky, A.
AU - Pelletier, É
DB - Scopus
DO - 10.1016/j.aquatox.2016.02.018
KW - Dissolved silver
Juveniles
Nanoparticles internalization
Organic coated silver nanoparticles toxicity
Sea urchin larvae
Animals
Ions
Larva
Metal Nanoparticles
Sea Urchins
Silver
Echinoidea
Invertebrata
quantum dot
sea water
silver nanoparticle
ion
metal nanoparticle
silver
water pollutant
echinoderm
juvenile
larva
mortality
nanoparticle
sublethal effect
toxicity
Article
confocal microscopy
controlled study
cytotoxicity
developmental stage
edema
exposure
food
immobility
juvenile animal
lethargy
mortality rate
nonhuman
peritoneum cell
priority journal
sea urchin
toxicity testing
animal
drug effects
metabolism
ultrastructure
M3 - Article
PY - 2016
SP - 208-227
ST - Physiological effects and cellular responses of metamorphic larvae and
juveniles of sea urchin exposed to ionic and nanoparticulate silver
T2 - Aquatic Toxicology
TI - Physiological effects and cellular responses of metamorphic larvae and
84960145395&doi=10.1016%2fj.aquatox.2016.02.018&partnerID=40&md5=3b50c27078864ab356
9f627446f7955a
VL - 174
ID - 5525
ER -
TY - JOUR
AB - The widespread use of silver nanoparticles (AgNPs) would likely result in
their discharge into wastewater and inevitable release in densely populated coastal
areas. It is known that AgNPs can cause harmful effects to marine fauna, but how
they affect development stages is still an open question. In order to understand in
details how polymer-coated AgNPs (PAAm-AgNPs) (from 0.19 to 4.64 mM as Ag) can
affect critical stages of marine invertebrate development, metamorphic larvae and
juveniles of sea urchins were used as biological models. Multidimensional scaling
(MDS) approach based on Bray-Curtis similarity matrix with PERMANOVA showed
organisms in a multivariate space undergoing through different physiological
conditions as a function of time, chemical forms of silver, nominal concentrations,
and presence or absence of food. Sublethal effects such as lethargy, oedema and
immobility mainly characterized PAAm-AgNPs effects with juveniles and postlarvae,
whereas necrosis and death arose in Ag+ conditions in short-term tests. Chronically
exposed metamorphic larvae had their morphogenic processes interrupted by PAAm-
AgNPs and a high mortality rate was observed in recovery period. On the contrary,
Ag+ ions caused progressive mortality during exposure, but a quick recovery in
uncontaminated seawater was observed. By means of fluorescent markers we showed
that nanosilver could be transferred between consecutive stages (swimming larvae
and postlarvae) and highlighted how important is food to enhance PAAm-AgNPs uptake.
Using TEM we observed that unfed juveniles had nanosilver aggregates mostly
restricted to their coelomic sinuses, while metamorphic larvae already had nano-
contamination overspread in different tissues and blastocoel. Our main hypothesis
for nanotoxicity of PAAM-AgNPs relies on the slow dissolution of nano-core over
time, but in this study the effects of particulate silver form itself are also
evoked. Main mechanisms governing tissular and cellular responses to nano-
intoxication such as inflammatory response and detoxification based on the role of
sentinel cells (peritoneal cells and coelomocytes) for general homeostasis are
discussed. This paper is first to detail physiological states, main uptake routes
and cellular response against polymer-coated AgNPs in developmental stages of
marine invertebrate species. (C) 2016 Elsevier B.V. All rights reserved.
AN - WOS:000374604100022
AU - Magesky, A.
AU - Pelletier, E.
DA - MAY
DO - 10.1016/j.aquatox.2016.02.018
PY - 2016
SN - 0166-445X
1879-1514
SP - 208-227
ST - Physiological effects and cellular responses of metamorphic larvae and
T2 - AQUATIC TOXICOLOGY
TI - Physiological effects and cellular responses of metamorphic larvae and
VL - 174
ID - 6159
ER -
TY - JOUR
AB - In the recent years usage of nanomedicine plays a promising strategy in the
improvement of medical treatment. The ecofriendly synthesized silver nanoparticles
has introduced a new opportunity to increase the efficacy of drug by reducing its
side effects. In the present study, we investigated the antioxidant property of
Bacopa monniera stabilized silver nanoparticles against aluminum induced toxicity
in albino mice. Forty male albino mice were randomly divided into five groups.
First group was treated as control, second group received aluminum acetate (5 mg/kg
b·w), third group received Bacopa monniera extract (5 mg/kg b·w), fourth group
received BmSNPs (5 mg/kg b·w), fifth group received aluminum acetate plus BmSNPs.
Exposure to aluminum acetate significantly increased lipid peroxidation levels with
a significant decrease in the antioxidant enzymes such as superoxide dismutase,
catalase and glutathione peroxidase activities in the brain, liver and kidney of
mice. Degenerative changes were also observed in brain, liver and kidney of
aluminum treated mice. No significant changes in the oxidative stress were observed
in the Bacopa monniera and BmSNPs alone treated mice. Whereas, co-administration of
BmSNPs to Al treated mice showed a significant decrease in lipid peroxidation
levels with a significant increase of SOD, CAT and GPx indicating the antioxidant
potential of nanoparticles and in counteracting Al induced oxidative stress and
histological response in male albino mice. These findings clearly implicate that
BmSNPs are able to eradicate the oxidative stress and prevent the tissue damage in
aluminum exposed mice. Copyright © 2015 American Scientific Publishers. All rights
reserved.
AU - Mahitha, B.
AU - Raju, B. D. P.
AU - Mallikarjuna, K.
AU - Mahalakshmi, Ch N. D.
AU - Sushma, N. J.
DB - Scopus
DO - 10.1166/jnn.2015.8995
IS - 2
KW - Aluminum
Bacopa monniera
Oxidative stress
Animals
Bacopa
Diffusion
Drug Stability
Drug Synergism
Excipients
Male
Metal Nanoparticles
Mice
Organ Specificity
Oxidative Stress
Particle Size
Plant Extracts
Silver
Systemic Inflammatory Response Syndrome
Tissue Distribution
Treatment Outcome
Antioxidants
Brain
Lipids
Mammals
Nanoparticles
Organometallics
Oxidation
Oxygen
Plants (botany)
Toxicity
Volatile fatty acids
aluminum
excipient
metal nanoparticle
plant extract
silver
Antioxidant potential
Degenerative changes
Glutathione peroxidase
Lipid peroxidation levels
Super oxide dismutase
animal
antibody specificity
chemically induced
chemistry
diffusion
drug effects
drug potentiation
drug stability
immunology
male
mouse
oxidative stress
particle size
systemic inflammatory response syndrome
tissue distribution
treatment outcome
ultrastructure
M3 - Article
PY - 2015
SP - 1101-1109
ST - Bacopa monniera stabilized silver nanoparticles attenuates oxidative stress
induced by aluminum in albino mice
TI - Bacopa monniera stabilized silver nanoparticles attenuates oxidative stress
induced by aluminum in albino mice
84920678752&doi=10.1166%2fjnn.2015.8995&partnerID=40&md5=ad4f9e00e4335ebb7444b60058
3ed3a6
VL - 15
ID - 5637
ER -
TY - JOUR
AB - Background: Hepatotoxicity was one of the major side effects associated with
doxorubicin treatment in cancer chemotherapy. The synthesized silver nanoparticles
(AgNPs) from natural products such as algae especially green algae is one of the
favorable means to minimize the deleterious effects of the chemotherapy. Thus, this
study aimed to evaluate the preventive role of AgNPs synthesized by Ulva fasciata
(U. fasciata) against doxorubicin-induced hepatotoxicity and oxidative stress in
the liver of male Wistar rats. Materials and Methods: In the present study, the
green macroalga U. fasciata ethanolic extract was used as reducing agents to reduce
Ag ions to Ag-0. Doxorubicin-injected male Wistar rats were concomitantly treated
with U. fasciata ethanolic extract and AgNPs synthesized by U. fasciata extract
(AgNPs/U. fasciata) 3 times/week by oral gavage for 6 weeks. Results: The results
showed that male Wistar rats injected with doxorubicin showed a significant
increase in ALT, ALP and GGT activities and total bilirubin level as well as a
reduction in the serum albumin level. The concurrent treatments of doxorubicin-
injected rats with U. fasciata ethanolic extract and AgNPs/U. fasciata
significantly abrogate these alterations. The altered levels of tumor biomarkers
CA19.9 and AFP as well as pro-inflammatory cytokine, TNF-alpha, and anti-
inflammatory cytokine, IL-4, in doxorubicin-injected animals were significantly
ameliorated by concurrent treatment with U. fasciata and AgNPs/U. fasciata.
Moreover, the elevated mRNA expression of p53 significantly decreased by treatment.
In association, the doxorubicin-induced deleterious histological changes
represented by severe hydropic degenerative changes, steatosis, inflammatory cell
infiltration, Kupffer cell proliferation and apoptosis were remarkably improved by
concurrent treatment with U. fasciata extract and AgNPs/U. fasciata which was more
potent. Conclusion: Based on results of this study, it can be concluded that U.
fasciata extract and AgNPs/U. fasciata counteracts doxorubicin-induced toxicity by
suppression of inflammation, oxidative stress and apoptosis. AgNPs/U. fasciata was
the most potent in improving hepatocyte integrity and liver histological
architecture.
AN - WOS:000669864500004
AU - Mahmoud, A. M.
AU - Ahmed, O. M.
AU - Mohamed, I. B.
AU - Soliman, H. A.
AU - Mohamed, B. M.
C7 - 67485
DO - 10.9734/JPRI/2021/v33i24A31429
IS - 24A
PY - 2021
SN - 2456-9119
SP - 24-48
ST - The Preventive Effects and Mode of Actions of Ulva Fasciata Synthesized
Silver Nanoparticles in Doxorubicin-Induced Hepatotoxicity in Wistar Rats
TI - The Preventive Effects and Mode of Actions of Ulva Fasciata Synthesized
Silver Nanoparticles in Doxorubicin-Induced Hepatotoxicity in Wistar Rats
VL - 33
ID - 6154
ER -
TY - JOUR
AB - Progressive accumulation of misfolded amyloid proteins in intracellular and
extracellular spaces is one of the principal reasons for synaptic damage and
impairment of neuronal communication in several neurodegenerative diseases.
Effective treatments for these diseases are still lacking but remain the focus of
much active investigation. Despite testing several synthesized compounds, small
molecules, and drugs over the past few decades, very few of them can inhibit
aggregation of amyloid proteins and lessen their neurotoxic effects. Recently, the
natural polyphenol curcumin (Cur) has been shown to be a promising anti-amyloid,
anti-inflammatory and neuroprotective agent for several neurodegenerative diseases.
Because of its pleotropic actions on the central nervous system, including
preferential binding to amyloid proteins, Cur is being touted as a promising
treatment for age-related brain diseases. Here, we focus on molecular targeting of
Cur to reduce amyloid burden, rescue neuronal damage, and restore normal cognitive
and sensory motor functions in different animal models of neurodegenerative
diseases. We specifically highlight Cur as a potential treatment for Alzheimer’s,
Parkinson’s, Huntington’s, and prion diseases. In addition, we discuss the major
issues and limitations of using Cur for treating these diseases, along with ways of
circumventing those shortcomings. Finally, we provide specific recommendations for
optimal dosing with Cur for treating neurological diseases. © 2018 by the authors.
AU - Maiti, P.
AU - Dunbar, G. L.
C7 - 1637
DB - Scopus
DO - 10.3390/ijms19061637
IS - 6
KW - Amyloidosis
Anti-amyloid
Curcumin
Molecular chaperones
Natural polyphenol
Neuroinflammation
Age Factors
Aging
Amyloid
Animals
Biological Products
Humans
Nanomedicine
Nanotechnology
Nerve Tissue
Neurodegenerative Diseases
Polyphenols
Signal Transduction
4,4' [(1 phenyl 1h pyrazole 3,5 diyl)diethene 2,1 diyl]bis(2 methoxyphenol)
adenine
alpha turmerone
amyloid protein
beta turmerone
calcium calmodulin dependent protein kinase
chitosan
copper
curcumin
curcuminoid
cyclodextrin
cytochrome
gold nanoparticle
guanine
hexahydrocurcumin
iron
monoamine oxidase inhibitor
octahydrocurcumin
phosphoprotein DARPP 32
piperine
polyphenol
polysaccharide
silver nanoparticle
tau interferon
tetrahydrocurcumin
turmerone
unclassified drug
unindexed drug
zinc
amyloid
biological product
age
allergy
apoptosis
biosynthesis
blood brain barrier
blood clotting disorder
brain hemorrhage
capillary endothelial cell
chest tightness
cholestasis
dermatitis
diarrhea
fluorescence
gastrointestinal symptom
gene expression
gene overexpression
lipid peroxidation assay
molecular biology
multiphoton microscopy
nausea
nervous system development
neuropathology
neurotoxicity
oligomerization
oxidative stress
pH
phagocytosis
protein aggregation
protein misfolding
rash
Review
signal transduction
synaptogenesis
temperature
aging
amyloidosis
animal
chemistry
dose response
drug effect
human
metabolism
nanomedicine
nanotechnology
nervous tissue
pathology
M3 - Review
PY - 2018
ST - Use of curcumin, a natural polyphenol for targeting molecular pathways in
treating age-related neurodegenerative diseases
TI - Use of curcumin, a natural polyphenol for targeting molecular pathways in
treating age-related neurodegenerative diseases
85047960181&doi=10.3390%2fijms19061637&partnerID=40&md5=ce1805a6903f528e3f0c086d0f5
9e596
VL - 19
ID - 5460
ER -
TY - JOUR
AB - The phytocomponent conjugated silver nanoparticles (AgNPs) have been
extensively explored for various therapeutic applications such as antimicrobial,
antioxidant, anticancer, anti-inflammatory, antidiabetic and anticoagulant effects.
The bio-conjugation of Ag-based nanomaterial with plant extracts reduces their
toxicity to biological systems and enhances their therapeutic effectiveness. The
diversity of phytochemicals or capping agents provided by the plant extracts and
the small size and large surface area of AgNPs permits maximum adsorption of these
capping agents onto their surfaces that further promote the therapeutic performance
of phytoconjugated AgNPs in various biomedical applications. The mechanistic action
involved in antimicrobial and anticancer functions of AgNPs is mainly dependent on
the induction of reactive oxygen species (ROS) resulting in cellular apoptosis and
necrosis. This review summarizes the recent studies of various plant extract
assisted synthesis of AgNPs, potential biomedical applications with the possible
mechanism of action and major shortcomings affecting their therapeutic efficacy. ©
2021
AU - Majeed, M.
AU - Hakeem, K. R.
AU - Rehman, R. U.
C7 - 132527
DB - Scopus
DO - 10.1016/j.chemosphere.2021.132527
KW - Antimicrobial
Bio-conjugation
Efficacy
Nanomaterial
Phytochemicals
Therapeutic
Toxicity
Antioxidants
Metal Nanoparticles
Plant Extracts
Silver
Trachinotus falcatus
Cell death
Metal nanoparticles
Microorganisms
Plant extracts
plant extract
silver nanoparticle
antiinfective agent
antioxidant
metal nanoparticle
silver
Anti-inflammatories
Capping agent
Phytochemical
Phytocomponents
Synergistic effect
Therapeutic Application
anticoagulant
nanoparticle
anticoagulation
Article
drug efficacy
drug potentiation
human
nonhuman
synthesis
M3 - Article
PY - 2022
ST - Synergistic effect of plant extract coupled silver nanoparticles in various
therapeutic applications- present insights and bottlenecks
T2 - Chemosphere
TI - Synergistic effect of plant extract coupled silver nanoparticles in various
therapeutic applications- present insights and bottlenecks
85116855608&doi=10.1016%2fj.chemosphere.2021.132527&partnerID=40&md5=e689c1d2182735
6cc941618e8ecbe90a
VL - 288
ID - 5101
ER -
TY - JOUR
AB - Noble metal nanoparticles, especially silver due to its antimicrobial
properties, are amongst the most widely used types of nanomaterials, and so the
possibility of an organism's exposure to them is relatively high. Excluding
injections, there are three natural routes they can accidentally enter the body the
skin, lungs and the alimentary tract. Research on rodents indicate that after
inhalation, injection or oral administration silver, gold and copper nanoparticles
can easily enter the systemic circulation and reach the internal organs.
Particularly vulnerable to the harmful effects of nanoparticles are organs with an
extended reticuloendothelial system, such as the spleen, where the accumulation of
nanoparticles occurs. It is well proved that metallic nanoparticles are easily
absorbed by macrophages located in lymphoid tissues but cannot be destroyed inside
the cells. They remain unchanged in phagosomes and chronically stimulate the cells
to pro-inflammatory cytokine production. They can also interact with other cell
types present in the local environment, e.g. lymphocytes, which can lead to an
inadequate immunological response of the organism. Many authors have described the
pro-inflammatory effect of noble metal nanoparticles, both local to the application
site and generalized. What is more, silver nanoparticles were able to disturb the
Th1/Th2 balance or even cause an allergic response of the organism. The beneficial
impact of silver nanoparticles on the immune response occurs only when they were
applied externally in the form of dressings or ointments in the treatment of
wounded or inflamed skin. In such cases nanosilver exhibited immunoregulatory
properties accelerating the healing. An explanation for this mode of action may be
the fact of relatively poor skin penetration by nanoparticles, limiting their
effect to the local tissues only.
AN - WOS:000333662200003
AU - Malaczewska, J.
DA - APR
IS - 4
PY - 2014
SN - 0025-8628
SP - 204-208
ST - Impact of noble metal nanoparticles on the immune system of animals
T2 - MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE
TI - Impact of noble metal nanoparticles on the immune system of animals
VL - 70
ID - 6317
ER -
TY - JOUR
AB - An increasing number of applications of silver nanoparticles in industry,
medicine and everyday life means that the risk of exposure of the human organism to
their potential harmful influence is growing. This study has sought to assess the
effect of 28-day alimentary administration of different concentrations (0.25, 2.5
and 25 ppm) of a commercial silver nanocolloid on the proliferative activity and
synthesis of cytokines by mouse splenocytes. All of the analyzed doses of the
colloid had a significant, albeit different, effect on the activity of splenocytes.
At the lowest dose, a significant decrease in the proliferation of T cells and more
intensive synthesis of pro-inflammatory cytokines, both by non-stimulated and LPS-
stimulated cells, was observed. The intermediate dose, on the other hand,
stimulated proliferation of B cells while producing a pro-inflammatory effect
regarding the synthesis of cytokines. Finally, the highest dose decreased the
synthesis of cytoldnes by non-stimulated cells, but after LPS stimulation, through
the strong activation of the IL-10 synthesis, it raised the proliferation of B
cells and decreased the synthesis of pro-inflammatory cytokines. The results
suggest that silver nanoparticles administered orally have an easy access to the
peripheral organs of the immune system, such as the spleen, but the effect of long-
term exposure of this organ to the effect of silver nanocolloid depends on several
factors, including the dose of nanoparticles, and seems as difficult to predict.
AN - WOS:000333322600004
AU - Malaczewska, J.
DO - 10.2478/pjvs-2014-0004
IS - 1
PY - 2014
SN - 1505-1773
2300-2557
SP - 27-35
ST - The splenocyte proliferative response and cytokine secretion in mice after
28-day oral administration of silver nanocolloid
T2 - POLISH JOURNAL OF VETERINARY SCIENCES
TI - The splenocyte proliferative response and cytokine secretion in mice after
28-day oral administration of silver nanocolloid
VL - 17
ID - 6238
ER -
TY - JOUR
AB - This study examines the formation of biofilm on biomaterials commonly used in
facial plastics and reconstruction including titanium, silicone, ionbombarded
silicone (Ultrasil), e-PTFE (Gore-Tex), e-PTFE with silver/chlorhexidine (Gore-Tex
Plus), and PHDPE (Medpor). These biomaterials were implanted subcutaneously in the
dorsum of 11 guinea pigs after contamination with Staphylococcus aureus and
examined with scanning electron microscopy after 7 days. Wounds were also inspected
for infection and extrusion rates. Results show biofilm formation on titanium,
silicone, ion-bombarded silicone, e-PTFE, and PHDPE associated with high rates of
extrusion and infection. Implants of e-PTFE with silver/chlorhexidine, on the other
hand, appeared resistant to biofilm formation and demonstrated significantly lower
rates of extrusion and infection. Contamination of bioimplants in vivo leads to
formation of bacterial biofilm on the surface of the biomaterial, causing
infection, pus formation, and extrusion. The authors hypothesize that the
antiseptic agents impregnated in the biomaterial form a protective coat of silver,
chlorhexidine, and inflammatory cells that inhibits initial bacterial adhesion to
the biomaterial surface. © 1998 American Laryngological, Rhinological and
Otalogical Society, Inc.
AU - Malaisrie, S. C.
AU - Malekzadeh, S.
AU - Biedlingmaier, J. F.
DB - Scopus
DO - 10.1097/00005537-199811000-00026
IS - 11
KW - Biofilm
bioimplant
biomaterial
Animals
Bacterial Adhesion
Biofilms
Chlorhexidine
Face
Guinea Pigs
Materials Testing
Polyethylenes
Prosthesis Failure
Prosthesis Implantation
Reconstructive Surgical Procedures
Silicones
Silver
Skin
Suppuration
Surface Properties
Surgical Wound Infection
Titanium
chlorhexidine
politef
silicone
silver
titanium
animal model
animal tissue
article
bacterium adherence
biocompatibility
biofilm
controlled study
face surgery
guinea pig
implant
infection rate
infectious complication
nonhuman
plastic surgery
priority journal
t tube
M3 - Article
PY - 1998
SP - 1733-1738
ST - In vivo analysis of bacterial biofilm formation on facial plastic bioimplants
T2 - Laryngoscope
TI - In vivo analysis of bacterial biofilm formation on facial plastic bioimplants
0031796663&doi=10.1097%2f00005537-199811000-
00026&partnerID=40&md5=6c73d185870fbc7e6b6b963a6a69271c
VL - 108
ID - 5767
ER -
TY - JOUR
AB - Nanostructured Ag@SiO2-Penicillin was synthesized from high-purity Ag0 NPs
with a mean particle size of about 10 nm produced by electromagnetic levitation gas
condensation (ELGC) method. The silver and penicillin contents of the synthesized
nano-antibiotic were about 34 wt% and 2.5 wt% respectively, as determined by ICP-
OES and TGA analyses. The antibacterial properties and synergistic effects of
nanostructured Ag@SiO2 and Ag@SiO2–Penicillin on killing the Methicillin-
susceptible S. aureus (MSSA) and Methicillin-resistant S. aureus (MRSA) bacteria
were also examined. The nanoparticles were characterized by X-ray diffraction (XRD)
and transmission electron microscopy (TEM). Ag@SiO2-Penicillin NPs showed an
outstanding antibacterial activity compared to Penicillin and Ag@SiO2 NPs. The
Fractional inhibitory concentration (FIC) indexes were 0.54 and 0.52 against MSSA
and MRSA bacteria respectively, illustrating the synergistic effects of Ag@SiO2-
Penicillin NPs. In addition, Ag@SiO2-Penicillin NPs showed promising dose-dependent
cytotoxicity effects indicating the protective effects emanating from anti-
inflammatory properties of penicillin. © 2019 Elsevier B.V.
AU - Malekzadeh, M.
AU - Yeung, K. L.
AU - Halali, M.
AU - Chang, Q.
DB - Scopus
DO - 10.1016/j.msec.2019.04.083
KW - Penicillin
Silica
Synergistic effect
Cell Death
Cell Survival
Electromagnetic Phenomena
Humans
Nanostructures
Nanotechnology
Particle Size
Penicillins
Silicon Dioxide
Silver
Static Electricity
Thermogravimetry
X-Ray Diffraction
Bacteria
Drug products
Electromagnetic propulsion
Levitation melting
Nanoparticles
Particle size
nanomaterial
silicon dioxide
silver
Anti-inflammatories
Cytotoxicity effects
Electromagnetic levitation
Inhibitory concentration
cell death
cell survival
chemistry
drug effect
electromagnetism
human
nanotechnology
particle size
procedures
static electricity
synthesis
thermogravimetry
ultrastructure
X ray diffraction
Silver compounds
M3 - Article
PY - 2019
SP - 616-622
ST - Synthesis of nanostructured Ag@SiO2-Penicillin from high purity Ag NPs
prepared by electromagnetic levitation melting process
TI - Synthesis of nanostructured Ag@SiO2-Penicillin from high purity Ag NPs
prepared by electromagnetic levitation melting process
85065037195&doi=10.1016%2fj.msec.2019.04.083&partnerID=40&md5=21d553929bd524d06b048
667786aac97
VL - 102
ID - 5432
ER -
TY - JOUR
AB - Aim of the study The present study was aimed to evaluate the anti-arthritic
effects of silver nanoparticles synthesised using Piper nigrum extract and to
further establish its mechanism of action in a rat model of adjuvant induced
arthritis (AA). Materials and methods Adjuvant arthritis was induced by injecting
complete Freund's adjuvant (0.1 mL) into the left hind paw of 36 albino Wistar rats
(n = 6). Silver nanoparticles stabilised with Piper nigrum extract (25 and
50 mg/kg). Commercial silver nanoparticles (50 mg/kg) and methotrexate (0.1 mg/kg)
were administered by intraperitoneal route from day 11 to day 22 on alternate days.
Results It was found that treatment with silver nanoparticles stabilised with Piper
nigrum (S-AgNPs) significantly reduced the paw edema and alleviated the
histopathological changes of cell infiltration, synovial hyperplasia, bone and
cartilage destruction. Furthermore, the phytostabilised silver nanoparticles (S-
AgNPs) inhibited the protein expression of NF-kβ p65 and TNF-α as evidenced by
immunohistochemistry analysis. Conclusion Our current findings suggest that silver
nanoparticles stabilised with Piper nigrum extract (S-AgNPs) have potent anti-
arthritic activity which is mediated by inhibition of TNF-α and suppression of pro-
inflammatory cytokines that are secreted in response to activated transcription
factors of NF-kβ. © 2016 Elsevier B.V.
AU - Mani, A.
AU - Vasanthi, C.
AU - Gopal, V.
AU - Chellathai, D.
DB - Scopus
DO - 10.1016/j.intimp.2016.10.013
KW - Adjuvant arthritis
NF-kβ
Piper nigrum
TNF-α
Animals
Arthritis, Experimental
Drug Stability
Edema
Foot Joints
Freund's Adjuvant
Fruit
Lymph Nodes
Male
Metal Nanoparticles
Phytotherapy
Piper
Plant Extracts
Rats, Wistar
Silver
Transcription Factor RelA
black pepper extract
creatinine
Freund adjuvant
methotrexate
silver nanoparticle
urea
metal nanoparticle
plant extract
silver
adjuvant arthritis
animal cell
animal experiment
animal model
animal tissue
antiarthritic activity
Article
bone erosion
cartilage degeneration
cell hyperplasia
cell infiltration
controlled study
drug mechanism
drug synthesis
hind paw
histopathology
hyperplasia
liver toxicity
male
nonhuman
particle size
paw edema
priority journal
protein expression
rat
zeta potential
animal
chemistry
drug effects
drug stability
edema
foot joint
fruit
lymph node
metabolism
pathology
phytotherapy
Piper (plant)
Wistar rat
M3 - Article
PY - 2016
SP - 17-23
ST - Role of phyto-stabilised silver nanoparticles in suppressing adjuvant induced
arthritis in rats
TI - Role of phyto-stabilised silver nanoparticles in suppressing adjuvant induced
arthritis in rats
84992364820&doi=10.1016%2fj.intimp.2016.10.013&partnerID=40&md5=a227ae49074dd35326f
0df080efe6399
VL - 41
ID - 5510
ER -
TY - JOUR
AB - The present study was aimed at biosynthesis of silver nanoparticles (AgNPs)
using ethanolic extract of rose (Rosa indica) petals and testing their potential
antibacterial activity using selective human pathogenic microbes, anticancer
activity using human colon adenocarcinoma cancer cell line HCT 15 as well as anti-
inflammatory activity using rat peritoneal macrophages in vitro. The biologically
synthesized AgNPs were also characterized by UV-visible spectroscopy, scanning
electron microscopy (SEM), transmission electron microscopy (TEM), Fourier
transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX)
and X-ray diffraction (XRD). The characterized AgNPs showed an effective
antibacterial activity against Gram negative (Escherichia coli, Klebsiella
pneumoniae) than Gram positive (Streptococcus mutans, Enterococcus faecalis)
bacteria. MTT assay, analysis of nuclear morphology, mRNA expression of Bcl-2, Bax
and protein expression of caspase 3 as well as 9, indicated potential anticancer
activity. In addition, green synthesized AgNPs also attenuated cytotoxicity,
nuclear morphology and free radical generation (O2- and NO) by rat peritoneal
macrophages in vitro. The results of our study show the potential green synthesis
of silver nanoparticles in mitigating their toxicity while retaining their
antibacterial activities. © 2014 Elsevier B.V. All rights reserved.
AU - Manikandan, R.
AU - Manikandan, B.
AU - Raman, T.
AU - Arunagirinathan, K.
AU - Prabhu, N. M.
AU - Jothi Basu, M.
AU - Perumal, M.
AU - Palanisamy, S.
AU - Munusamy, A.
DB - Scopus
DO - 10.1016/j.saa.2014.10.043
Antibacterial
Anticancer
Rosa indica
Animals
Bacteria
Cell Line, Tumor
Cells, Cultured
Colonic Neoplasms
Ethanol
Humans
Macrophages, Peritoneal
Male
Metal Nanoparticles
Plant Extracts
Plant Leaves
Rats
Rosa
Silver
Biochemistry
Biosynthesis
Cell culture
Escherichia coli
Free radicals
Macrophages
Morphology
Nanoparticles
X ray diffraction
alcohol
antiinfective agent
metal nanoparticle
plant extract
silver
Anti-inflammatories
animal
bacterium
cell culture
chemistry
drug effects
human
male
plant leaf
rat
rose
tumor cell line
ultrastructure
M3 - Article
PY - 2015
SP - 120-129
ST - Biosynthesis of silver nanoparticles using ethanolic petals extract of Rosa
indica and characterization of its antibacterial, anticancer and anti-inflammatory
activities
T2 - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
TI - Biosynthesis of silver nanoparticles using ethanolic petals extract of Rosa
activities
84919389652&doi=10.1016%2fj.saa.2014.10.043&partnerID=40&md5=dc015387b8fb38e75f2904
5a621b99f7
VL - 138
ID - 5648
ER -
TY - JOUR
AB - Biologically inspired synthesis of nanoparticles was found to be more
attractive in metal nanoparticle synthesis. The present study reported an in-situ
biogenic synthesis of silver nanoparticles (AgNPs) using Solanum trilobatum aqueous
leaf extract. On this basis, the aqueous leaf extract of S. trilobatum acted as a
reducing agent and stabilizing agent to synthesize highly stable AgNPs at ambient
temperature. Eventually, the synthesized and stabilized AgNPs surface plasmon
resonance was near 430 nm through a UV-visible (UV-vis) spectrophotometer. Here,
the stability of the silver colloids monitored through zeta potential and mean
particle size was evaluated through diffraction light scattering (DLF). Further,
the average particle size was found to be 27.6 nm and spherical, confirmed with
transmission electron microscopy (TEM). Also, colloidal AgNPs and aqueous extract
are found to be rich sources of antioxidants and exhibit higher free radical
scavenging ability. Thus, efficient inhibition with COX1 and COX2 enzymes and the
protective effect with human red blood cell (HRBC) membrane stability showed
significant results. These features are promising, suggesting the possibility of
the AgNPs to be useful to disease-modifying for treating inflammatory disorders and
associated complications. (C) 2021 The Author(s). Published by Elsevier B.V. on
behalf of King Saud University.
AN - WOS:000798983400020
AU - Manimegalai, S.
AU - Rajeswari, V. D.
AU - Parameswari, R.
AU - Nicoletti, M.
AU - Alarifi, S.
AU - Govindarajan, M.
C6 - MAR 2022
DA - APR
DO - 10.1016/j.sjbs.2021.11.048
IS - 4
PY - 2022
SN - 1319-562X
2213-7106
SP - 2131-2137
ST - Green synthesis, characterization and biological activity of Solanum
trilobatum-mediated silver nanoparticles
TI - Green synthesis, characterization and biological activity of Solanum
trilobatum-mediated silver nanoparticles
VL - 29
ID - 6178
ER -
TY - JOUR
AB - The primary objective of the current investigation was the biosynthesis of
Phy-AgNPs by the endophytic fungus Phyllosticta owaniana (extracted from Abrus
precatorius) and the evaluation of the secondary metabolites from the ethyl acetate
extract of P. owaniana cultivated by submerged fermentation. Utilizing
bioanalytical strategies, Phy-AgNPs were characterized. The UV–visible
spectrophotometer analysis revealed an absorption spectrum with a peak at 420 nm,
thus validating the Phy-AgNPs synthesis. The FTIR analysis revealed peaks
correlating to various potential functional groups, suggesting that Phy-AgNPs have
been reduced and capped. SEM-EDAX and HR-TEM analyses demonstrated the spherical
shape of Phy-AgNPs, and the 3 keV EDAX analysis confirmed the existence of silver
atoms. XRD analyses showed the Phy-AgNPs crystalline structure. The size and the
stability of synthesized Phy-AgNPs (65.81 nm) were measured by DLS and Zeta
potential studies. While the ethyl acetate extract was analyzed with GC–MS and FTIR
for secondary metabolites. The synthesized Phy-AgNPs showed effective antibacterial
activity against Pseudomonas aeruginosa (15.1 ± 0.17 mm, 10 mg/mL), while the
antifungal activity of Phy-AgNPs inhibited the growth of Candida albicans extremely
efficiently (12.16 ± 0.28 mm, 10 mg/mL). Phy-AgNPs were evaluated for a variety of
biomedical properties in which they showed significant activity. In a cell
viability assay using the MTT assay, Phy-AgNPs exhibited a cytotoxic impact of up
to 30.67% and 34.53% when 200 µg/mL were detected. In both in vitro and in vivo
anti-inflammatory examinations, nanoparticles (NPs) exhibited a significant anti-
inflammatory effect. These findings support the pharmaceutical and biomedical
properties of the synthesized Phy-AgNPs. © 2023, The Author(s), under exclusive
licence to Springer-Verlag GmbH Germany, part of Springer Nature.
AU - Manjunatha, D.
AU - Megha, G. T.
AU - Nagaraju, S.
AU - Akarsh, S.
AU - Nandish, G.
AU - Sowmya, H. V.
AU - Thippeswamy, B.
C7 - 217
DB - Scopus
DO - 10.1007/s00203-023-03549-1
IS - 5
KW - Antimicrobial properties
Endophytic fungus
GC–MS
Phy-AgNPs
Phyllosticta owaniana
Fungi
Metal Nanoparticles
Plant Extracts
Silver
amoxicillin
ampicillin
ascorbic acid
cefpodoxime
chloramphenicol
ciprofloxacin
cisplatin
diclofenac
fluconazole
functional group
fungal extract
gentamicin
imipenem
silver nanoparticle
silver nitrate
streptomycin
antiinfective agent
metal nanoparticle
plant extract
silver
Abrus precatorius
absorption spectroscopy
animal experiment
animal model
animal tissue
antifungal activity
Article
Aspergillus brasiliensis
Aspergillus flavus
bacterial growth
bacterium culture
biosynthesis
Candida albicans
cell viability
controlled study
crystal structure
cytotoxicity
drug synthesis
endophytic fungus
Escherichia coli
evaluation study
female
hemolysis
high resolution transmission electron microscopy
histopathology
human
human cell
in vitro study
in vivo study
molecular fingerprinting
mouse
MTT assay
muscle hypotonia
nonhuman
particle size
protein denaturation
secondary metabolism
submerged fermentation
X ray diffraction
zeta potential
zone of inhibition
chemistry
fungus
M3 - Article
PY - 2023
ST - Eco-friendly synthesized silver nanoparticles from endophytic fungus
Phyllosticta owaniana: KUMBMDBT-32 and evaluation of biomedical properties
T2 - Archives of Microbiology
TI - Eco-friendly synthesized silver nanoparticles from endophytic fungus
Phyllosticta owaniana: KUMBMDBT-32 and evaluation of biomedical properties
85159542280&doi=10.1007%2fs00203-023-03549-
1&partnerID=40&md5=f2833c74308d018dca22aa48e31a4307
VL - 205
ID - 5024
ER -
TY - JOUR
AB - Nanoparticles (NP) are highly applicable in a variety of technological and
biomedical fields because of their unique physicochemical properties. The increased
development and utilization of NP has amplified human exposure and raised concerns
regarding their potential to generate toxicity. The biological impacts of NP
exposures have been shown to be dependent on aerodynamic size, chemical
composition, and the route of exposure (oral, dermal, intravenous, and inhalation),
while recent research has demonstrated the cardiovascular (CV) system as an
important site of toxicity. Proposed mechanisms responsible for these effects
include inflammation, oxidative stress, autonomic dysregulation, and direct
interactions of NP with CV cells. Specifically, NP have been shown to impact
vascular endothelial cell (EC) integrity, which may disrupt the dynamic endothelial
regulation of vascular tone, possibly altering systemic vascular resistance and
impairing the appropriate distribution of blood flow throughout the circulation.
Cardiac consequences of NP-induced toxicity include disruption of heart rate and
electrical activity via catecholamine release, increased susceptibility to
ischemia/reperfusion injury, and modified baroreceptor control of cardiac function.
These and other CV outcomes likely contribute to adverse health effects promoting
myocardial infarction, hypertension, cardiac arrhythmias, and thrombosis. This
review will assess the current knowledge regarding the principle sites of CV
toxicity following NP exposure. Furthermore, we will propose mechanisms
contributing to altered CV function and hypothesize possible outcomes resulting in
decrements in human health. © 2012 Wiley Periodicals, Inc.
AU - Mann, E. E.
AU - Thompson, L. C.
AU - Wingard, C. J.
DB - Scopus
DO - 10.1002/wnan.1194
IS - 6
KW - Animals
Cardiovascular Diseases
Cardiovascular Physiological Phenomena
Cardiovascular System
Humans
Inhalation Exposure
Nanoparticles
Blood pressure
Diseases
Endothelial cells
advanced glycation end product receptor
catecholamine
cerium oxide
G protein coupled receptor
interleukin 1beta
interleukin 6
iron oxide
messenger RNA
nanoparticle
nickel complex
nitric oxide
prostaglandin E2
silicon dioxide
silver nanoparticle
thromboxane A2
titanium dioxide
zinc oxide
Adverse health effects
Aerodynamic sizes
Biological impacts
Biomedical fields
Blood flow
Cardiac arrhythmia
Cardiac functions
Cardio-pulmonary function
Chemical compositions
Direct interactions
Electrical activities
Heart rates
Human exposures
Human health
Ischemia/reperfusion
Myocardial Infarction
Vascular endothelial cells
Vascular tones
article
autonomic dysfunction
blood flow
blood vessel tone
cardiopulmonary function
cardiotoxicity
catecholamine release
cytotoxicity
electric activity
exposure
health hazard
heart arrhythmia
heart muscle ischemia
heart rate
human
hypertension
inflammation
lung toxicity
nonhuman
outcome assessment
oxidative stress
particle size
pressoreceptor
priority journal
reperfusion injury
thrombosis
vascular endothelium
vascular resistance
vascular smooth muscle
Toxicity
M3 - Article
PY - 2012
SP - 691-702
ST - Changes in cardiopulmonary function induced by nanoparticles
TI - Changes in cardiopulmonary function induced by nanoparticles
84867698123&doi=10.1002%2fwnan.1194&partnerID=40&md5=5dc35bb5ba9ee2fe791d9c164fb535
c3
VL - 4
ID - 5679
ER -
TY - JOUR
AB - Nanoparticles (NPs) are tiny materials used in a wide range of industrial and
medical applications. Titanium dioxide (TiO2) is a type of nanoparticle that is
widely used in paints, pigments, and cosmetics; however, little is known about the
impact of TiO2 on human health and the environment. Therefore, considerable
research has focused on characterizing the potential toxicity of nanoparticles such
as TiO2 and on understanding the mechanism of TiO2 NP-induced nanotoxicity through
the evaluation of biomarkers. Uncoated TiO2 NPs tend to aggregate in aqueous media,
and these aggregates decrease cell viability and induce expression of stress-
related genes, such as those encoding interleukin-6 (IL-6) and heat shock protein
70B' (HSP70B'), indicating that TiO2 NPs induce inflammatory and heat shock
responses. In order to reduce their toxicity, we conjugated TiO2 NPs with
polyethylene glycol (PEG) to eliminate aggregation. Our findings indicate that
modifying TiO2 NPs with PEG reduces their cytotoxicity and reduces the induction of
stress-related genes. Our results also suggest that TiO2 NP-induced effects on
cytotoxicity and gene expression vary depending upon the cell type and surface
modification.
AN - WOS:000302174500069
AU - Mano, S. S.
AU - Kanehira, K.
AU - Sonezaki, S.
AU - Taniguchi, A.
DA - MAR
DO - 10.3390/ijms13033703
IS - 3
PY - 2012
SN - 1422-0067
SP - 3703-3717
ST - Effect of Polyethylene Glycol Modification of TiO2 Nanoparticles on
Cytotoxicity and Gene Expressions in Human Cell Lines
TI - Effect of Polyethylene Glycol Modification of TiO2 Nanoparticles on
Cytotoxicity and Gene Expressions in Human Cell Lines
VL - 13
ID - 6189
ER -
TY - JOUR
AB - The rapid increase of incorporating silver nanoparticles (Ag-NPs) in
different anthropogenic and industrial activities increased the discharge of these
particles in the aquatic ecosystem. The environmental impact of Ag-NPs, especially
the green synthesized is still not completely understood on fish. Therefore, this
study aimed to investigate the effects of exposure to graded series of starch-
mediated Ag-NPs at levels of 0, 3.31, 6.63, 13.25, and 26.50 mg L−1 representing 0,
6.25, 12.5, 25, and 50% of LC50 on Nile tilapia (O. niloticus), respectively. Fish
with initial weight 37.63 ± 0.41 g were maintained in 70 L glass aquaria and
exposed to starch-mediated Ag-NPs (average particle size 40 nm) for 28 days. The
results revealed that starch-mediated Ag-NPs induced severe changes in the mRNA
levels of toxicity (CYP1A and Hsp70) and inflammatory (TNF-α and TGF-β) genes. The
expression of antioxidant genes (SOD and CAT) was significantly suppressed, and the
activities of their enzymes were inhibited significantly upon exposure.
Simultaneously, the malondialdehyde level increased significantly with increasing
the exposure levels of starch-mediated Ag-NPs. The red blood cells, hemoglobin,
hematocrit and white blood cell values were decreased significantly with doses over
3.31 mg L−1 of Ag-NPs. In addition, the total protein and globulin decreased
significantly with increasing Ag-NPs in a dose-dependent manner. The liver function
enzymes and kidney function indicators revealed severe toxicity with Ag-NPs
exposure. In conclusion, the effect of starch-mediated Ag-NPs in doses over 3.31 mg
L−1 induced obvious toxicity in the molecular and proteomic levels in Nile tilapia
fingerlings. © 2021
AU - Mansour, W. A. A.
AU - Abdelsalam, N. R.
AU - Tanekhy, M.
AU - Khaled, A. A.
AU - Mansour, A. T.
C7 - 109068
DB - Scopus
DO - 10.1016/j.cbpc.2021.109068
KW - Green synthesized Ag-NPs
Inflammatory
Nile tilapia
Oxidative stress
Physiological responses
Toxicity
Animals
Cichlids
Metal Nanoparticles
Oxidative Stress
Silver
antioxidant
catalase
cytochrome P450 1A
globulin
hemoglobin
malonaldehyde
messenger RNA
oligonucleotide
silver nanoparticle
starch
metal nanoparticle
silver
animal experiment
aquatic environment
Article
body weight gain
enzyme activity
erythrocyte
feed conversion ratio
fingerling
gene
gene expression
hematocrit
hematological parameters
hematology
kidney function
LC50
leukocyte
liver function
nonhuman
particle size
priority journal
survival
synthesis
X ray diffraction
animal
cichlid
drug effect
green chemistry
metabolism
oxidative stress
procedures
toxicity
water pollutant
M3 - Article
PY - 2021
ST - Toxicity, inflammatory and antioxidant genes expression, and physiological
changes of green synthesis silver nanoparticles on Nile tilapia (Oreochromis
niloticus) fingerlings
TI - Toxicity, inflammatory and antioxidant genes expression, and physiological
changes of green synthesis silver nanoparticles on Nile tilapia (Oreochromis
niloticus) fingerlings
85104921756&doi=10.1016%2fj.cbpc.2021.109068&partnerID=40&md5=0f5700c01de1f90e2870b
2628af6229f
VL - 247
ID - 5211
ER -
TY - JOUR
AB - Nano metal oxides have been proposed as alternatives to silver (Ag)
nanoparticles (NPs) for antibacterial coatings. Here, cotton and polyester-cotton
fabrics were sonochemically coated with zinc oxide (ZnO) and copper oxide (CuO)
NPs. By varying the reaction solvent (water or ethanol), NPs with different sizes
and shapes were synthesized. The cytotoxic and pro-inflammatory effects of studied
NPs were investigated in vitro in human alveolar epithelial A549 and macrophage-
like THP1 cells. To understand the potential respiratory impact of the NPs, the
coated textiles were subjected to the abrasion tests, and the released airborne
particles were measured. A very small amount of the studied metal oxides NPs was
released from abrasion of the textiles coated by the ethanol-based sonochemical
process. The release from the water-based coating was comparably higher. Lung and
immune cells viability decreased after 24 h of exposure only at the highest studied
NPs concentration (100 μg/mL). Different from the ZnO NPs, both formulations of CuO
NPs induced IL-8 release in the lung epithelial cells already at subtoxic
concentrations (1-10 μg/mL) but not in immune cells. All of the studied NPs did not
induce IL-6 release by the lung and immune cells. Calculations revealed that the
exposures of the NPs to human lung due to the abrasion of the textiles were lower
or comparable to the minimum doses in the cell viability tests (0.1 μg/mL), at
which acute cytotoxicity was not observed. The results alleviate the concerns
regarding the potential risk of these metal oxide NPs in their applications for the
textile coating and provide insight for the safe-by-design approach. © 2017
AU - Mantecca, P.
AU - Kasemets, K.
AU - Deokar, A.
AU - Perelshtein, I.
AU - Gedanken, A.
AU - Bahk, Y. K.
AU - Kianfar, B.
AU - Wang, J.
DB - Scopus
DO - 10.1021/acs.est.7b02390
IS - 16
KW - Alveolar Epithelial Cells
Copper
Humans
Macrophages
Metal Nanoparticles
Oxides
Silver
Textiles
Zinc Oxide
Gossypium hirsutum
Abrasion
Air pollution control
Biological organs
Cells
Coatings
Cotton
Cytology
Cytotoxicity
Ethanol
Metal nanoparticles
Metals
Organic solvents
Tribology
Zinc oxide
interleukin 6
interleukin 8
metal oxide
nanoparticle
polyester
silver nanoparticle
copper
cuprous oxide
metal nanoparticle
oxide
silver
zinc oxide
Airborne nanoparticles
Antibacterial coatings
Design approaches
Lung epithelial cells
Nanoparticle (NPs)
Polyester cotton fabric
Sonochemical process
Water-based coating
coating
design method
solvent
toxicity test
toxicology
zinc
airborne particle
aqueous solution
Article
cell viability
cytokine release
human
human cell
in vitro study
macrophage
nanotoxicology
particle size
risk factor
surface tension
synthesis
textile
Copper oxides
M3 - Article
PY - 2017
SP - 9305-9317
ST - Airborne Nanoparticle Release and Toxicological Risk from Metal-Oxide-Coated
Textiles: Toward a Multiscale Safe-by-Design Approach
TI - Airborne Nanoparticle Release and Toxicological Risk from Metal-Oxide-Coated
85027446433&doi=10.1021%2facs.est.7b02390&partnerID=40&md5=a494fa7d1315f568dcdc54d3
a22e5f7c
VL - 51
ID - 5511
ER -
TY - JOUR
AB - Nano metal oxides have been proposed as alternatives to silver (Ag)
nanoparticles (NPs) for antibacterial coatings. Here, cotton and polyester-cotton
fabrics were sonochemically coated with zinc oxide (ZnO) and copper oxide (CuO)
NPs. By varying the reaction solvent (water or ethanol), NPs with different sizes
and shapes were synthesized. The cytotoxic and pro-inflammatory effects of studied
NPs were investigated in vitro in human alveolar epithelial A549 and macrophage-
like THP1 cells. To understand the potential respiratory impact of the NPs, the
coated textiles were subjected to the abrasion tests, and the released airborne
particles were measured. A very small amount of the studied metal oxides NPs was
released from abrasion of the textiles coated by the ethanol-based sonochemical
process. The release from the water-based coating was comparably higher. Lung and
immune cells viability decreased after 24 h of exposure only at the highest studied
NPs concentration (100 mu g/mL). Different from the ZnO NPs, both formulations of
CuO NPs induced IL-8 release in the lung epithelial cells already at subtoxic
concentrations (1-10 mu g/mL) but not in immune cells. All of the studied NPs did
not induce IL-6 release by the lung and immune cells. Calculations revealed that
the exposures of the NPs to human lung due to the abrasion of the textiles were
lower or comparable to the minimum doses in the cell viability tests (0.1 mu g/mL),
at which acute cytotoxicity was not observed. The results alleviate the concerns
regarding the potential risk of these metal oxide NPs in their applications for the
textile coating and provide insight for the safe-by-design approach.
AN - WOS:000407987400047
AU - Mantecca, P.
AU - Kasemets, K.
AU - Deokar, A.
AU - Perelshtein, L.
AU - Gedanken, A.
AU - Bahk, Y. K.
AU - Kianfar, B.
AU - Wang, J.
DA - AUG 15
DO - 10.1021/acs.est.7b02390
IS - 16
PY - 2017
SN - 0013-936X
1520-5851
SP - 9305-9317
ST - Airborne Nanoparticle Release and Toxicological Risk from Metal-Oxide-Coated
T2 - ENVIRONMENTAL SCIENCE & TECHNOLOGY
TI - Airborne Nanoparticle Release and Toxicological Risk from Metal-Oxide-Coated
VL - 51
ID - 6704
ER -
TY - JOUR
AB - Background:This investigation aimed to evaluate the occurrence of some
apoptotic features induced by Leptospira interrogans serovar Icterohaemorrhagiae
infection in young BALB/c mice during 2, 4, 7, 10, 14 and 21 days post-infection
(dpi).Methods:The animals were euthanized and lung, liver and kidneys were
harvested to histopathology analysis and immunohistochemistry to caspase-3 antigen
detection was performed.Results:Chromatin condensation in kidney and liver tissues,
but not in lung tissue, was observed. Caspase-3 reactive cells, mainly
characterized as renal epithelial cells, were detected in the days 14 and 21 at
high levels when compared to days 2,4 and 7 (p = 0.025; p <0.05). Lung sections
revealed caspase-3 labeled alveolar cells in 10 and 14 days post-infection was
higher than observed at 7 days (p = 0.0497; p < 0.05). Liver sections demonstrated
reactive cells at a highest level at 14 and 21 days post-infection when comparison
to 2,4, 7 and 10 days (p = 0.0069; p<0.05).Conclusions:Our results suggest that
infection of L interrogans induce in kidney, liver and lung an activation of
apoptosis mediated by caspase-3 dependent pathway in later phases of infectious
process.(AU)
AD - Marinho, Márcia
Universidade Estadual Paulista. Veterinary Medicine School. Department of Support,
Production and Animal Health. Araçatuba. BR
Táparo, Cilene Vidovix
Oliveira Júnior, Itamar S
Universidade Federal de São Paulo. Department of Inflammatory Mediators,
Anesthesiology, Pain and Intensive Care. São Paulo. BR
Perri, Silvia Helena Venturoli
Cardoso, Tereza Cristina
AU - Marinho, Márcia
AU - Táparo, Cilene Vidovix
AU - Oliveira Júnior, Itamar S.
AU - Perri, Silvia Helena Venturoli
AU - Cardoso, Tereza Cristina
C1 - 20180817
DA - 2015/00
DB - LILACS
DO - 10.1186/s40409-015-0022-y
KW - Caspases
Leptospirosis
Mice
Programmed cell death
LA - en
PY - 2015
SN - 1678-9199
SP - 22-22
ST - Tissue apoptosis in mice infected with Leptospira interrogans serovar
Icterohaemorrhagiae
T2 - J. venom. anim. toxins incl. trop. dis
TI - Tissue apoptosis in mice infected with Leptospira interrogans serovar
Icterohaemorrhagiae
UR - https://www.scielo.br/j/jvatitd/a/MHJhLZNZdn7Mb3p7rnCzGRb/?format=pdf〈=en
VL - 21
ID - 4937
ER -
TY - JOUR
AB - Recent studies demonstrated that inhibitors of pro-inflammatory molecular
cascades triggered by rabies infection in the central nervous system (CNS) can
enhance survival in mouse model and that certain antiviral compounds interfere with
rabies virus replication in vitro. In this study different combinations of
therapeutics were tested to evaluate their effect on survival in rabies-infected
mice, as well as on viral load in the CNS. C57Bl/6 mice were infected with Silver-
haired bat rabies virus (SHBRV)-18 at virus dose approaching LD50 and LD100. In one
experimental group daily treatments were initiated 4 h before-, in other groups 48
or 96 h after challenge. In the first experiment therapeutic combination contained
inhibitors of tumour necrosis factor-α (infliximab), caspase-1 (Ac-YVAD-cmk), and a
multikinase inhibitor (sorafenib). In the treated groups there was a notable but
not significant increase of survival compared to the virus infected, non-treated
mice. The addition of human rabies immunoglobulins (HRIG) to the combination in the
second experiment almost completely prevented mortality in the pre-exposure
treatment group along with a significant reduction of viral titres in the CNS.
Post-exposure treatments also greatly improved survival rates. As part of the
combination with immunomodulatory compounds, HRIG had a higher impact on survival
than alone. In the third experiment the combination was further supplemented with
type-I interferons, ribavirin and favipiravir (T-705). As a blood-brain barrier
opener, mannitol was also administered. This treatment was unable to prevent lethal
consequences of SHBRV-18 infection; furthermore, it caused toxicity in treated
mice, presumably due to interaction among the components. In all experiments, viral
loads in the CNS were similar in mice that succumbed to rabies regardless of
treatment. According to the findings, inhibitors of detrimental host response to
rabies combined with antibodies can be considered among the possible therapeutic
and post-exposure options in human rabies cases. © 2018 Elsevier Ltd
AU - Marosi, A.
AU - Dufkova, L.
AU - Forró, B.
AU - Felde, O.
AU - Erdélyi, K.
AU - Širmarová, J.
AU - Palus, M.
AU - Hönig, V.
AU - Salát, J.
AU - Tikos, R.
AU - Gyuranecz, M.
AU - Růžek, D.
AU - Martina, B.
AU - Koraka, P.
AU - Osterhaus, A. D. M. E.
AU - Bakonyi, T.
DB - Scopus
DO - 10.1016/j.vaccine.2018.05.066
IS - 33
KW - Combination
HRIG
Infliximab
Rabies virus
Sorafenib
Survival
Animals
Antibodies, Viral
Antiviral Agents
Body Weight
Female
Immunoglobulins
Kaplan-Meier Estimate
Mice
Mice, Inbred C57BL
Rabies
Virus Replication
acetyltyrosylvalylalanylaspartic acid chloromethylketone
alpha interferon
antivirus agent
beta interferon
favipiravir
infliximab
interferon
rabies immunoglobulin
ribavirin
sorafenib
unclassified drug
immunoglobulin
virus antibody
animal experiment
animal model
animal tissue
Article
blood brain barrier
C57BL 6 mouse
combination drug therapy
controlled study
drug antagonism
female
immune response
immunomodulation
LD100
LD50
monotherapy
mortality
mouse
nonhuman
priority journal
rabies
survival rate
virus load
animal
body weight
C57BL mouse
disease model
drug effect
immunology
Kaplan Meier method
pathogenicity
virus replication
M3 - Article
PY - 2019
SP - 4724-4735
ST - Combination therapy of rabies-infected mice with inhibitors of pro-
inflammatory host response, antiviral compounds and human rabies immunoglobulin
T2 - Vaccine
TI - Combination therapy of rabies-infected mice with inhibitors of pro-
inflammatory host response, antiviral compounds and human rabies immunoglobulin
85047466499&doi=10.1016%2fj.vaccine.2018.05.066&partnerID=40&md5=c9094d4a0a7042bb3f
0bd48529cc96a0
VL - 37
ID - 5454
ER -
TY - JOUR
AB - PURPOSE: To evaluate intestinal inflammatory and apoptotic processes after
intestinal ischemia/reperfusion injury, modulated by pentoxifylline and hypertonic
saline. METHODS: It was allocated into four groups (n=6), 24 male Wistar rats (200
to 250g) and submitted to intestinal ischemia for 40 min and reperfusion for 80
min: IR (did not receive any treatment); HS group (Hypertonic Saline, 4ml/kg-IV);
PTX group (Pentoxifylline, 30mg/kg-IV); HS+PTX group (Hypertonic Saline and
Pentoxifylline). All animals were heparinized (100U/kg). At the end of reperfusion,
ileal fragments were removed and stained on hematoxylin-eosin and histochemical
studies for COX-2, Bcl-2 and cleaved caspase-3. RESULTS: The values of sO2 were
higher on treated groups at 40 minutes of reperfusion (p=0.0081) and 80 minutes of
reperfusion (p=0.0072). Serum lactate values were lower on treated groups after 40
minutes of reperfusion (p=0.0003) and 80 minutes of reperfusion (p=0.0098).
Morphologic tissue injuries showed higher grades on IR group versus other groups:
HS (p=0.0006), PTX (p=0.0433) and HS+PTX (p=0.0040). The histochemical study showed
lesser expression of COX-2 (p=0.0015) and Bcl-2 (p=0.0012) on HS+PTX group. A lower
expression of cleaved caspase-3 was demonstrated in PTX (p=0.0090; PTXvsIR).
CONCLUSION: The combined use of pentoxifylline and hypertonic saline offers best
results on inflammatory and apoptotic inhibitory aspects after intestinal
ischemia/reperfusion. .
AD - Marques, Geraldo Magela Nogueira
Sao Paulo University. Medical School. BR
Rasslan, Roberto
Belon, Alessandro Rodrigo
Carvalho, Juliana Gonçalves
Felice Neto, Raphael
Rasslan, Samir
Utiyama, Edivaldo Massazo
Montero, Edna Frasson de Souza
AU - Marques, Geraldo Magela Nogueira
AU - Rasslan, Roberto
AU - Belon, Alessandro Rodrigo
AU - Carvalho, Juliana Gonçalves
AU - Felice Neto, Raphael
AU - Rasslan, Samir
AU - Utiyama, Edivaldo Massazo
AU - Montero, Edna Frasson de Souza
C1 - 20141128
DA - 2014/11
DB - LILACS
DO - 10.1590/S0102-86502014001800007
IS - 11
KW - Apoptosis
Inflammation
Ischemia
Pentoxifylline
Rats
Reperfusion
Saline Solution, Hypertonic
LA - en
PY - 2014
SN - 0102-8650
SP - 735-741
ST - Pentoxifylline associated to hypertonic saline solution attenuates
inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats
T2 - Acta cir. bras
TI - Pentoxifylline associated to hypertonic saline solution attenuates
inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats
86502014001100735
VL - 29
ID - 4938
ER -
TY - JOUR
AB - Background Indian-origin rhesus (InR) are preferred for research, but strict
export restrictions continue to limit their use. Chinese-origin rhesus (ChR),
although easier to procure, are genetically distinct from InR and differ in their
immune response to infectious agents, such as the Simian Immunodeficiency Virus.
The most advanced malaria vaccine, RTS,S (GlaxoSmithKline), is based on the
circumsporozoite protein (CSP) of Plasmodium falciparum. The efficacy of RTS,S
vaccine in the field remains low and short-lived; efforts are underway to improve
CSP-based vaccines. Rhesus models can accelerate preclinical down-selection of the
next generation of malaria vaccines. This study was used to determine if the safety
and immunogenicity outcomes following vaccination with a CSP vaccine would differ
in the InR and ChR models, given the genetic differences between the two sub-
populations of rhesus. Methods The FMP013 vaccine, was composed of nearly full-
length soluble P. falciparum CSP produced in Escherichia coli and was adjuvanted
with the Army liposomal formulation (ALFQ). Three doses of the vaccine were
administered in InR and ChR (n = 6) at 1-month intervals and the antibody and T
cell responses were assessed. Results Local and systemic toxicity profile of FMP013
vaccine in InR and ChR were similar and they revealed that the FMP013 vaccine was
safe and caused only mild and transient inflammatory adverse reactions. Following
the first 2 vaccines, there was a slower acquisition of antibodies to the CSP
repeat region in ChR. However after the 3rd vaccination the titers in the two
models were comparable. The ChR group repeat-specific antibodies had higher avidity
and ChR group showed higher inhibition of liver stage development activity compared
to InR. There was no difference in T-cell responses to the FMP013 vaccine between
the two models. Conclusions A difference in the quality of serological responses
was detected between the two sub-populations of rhesus. However, both models
confirmed that FMP013/ALFQ vaccine was safe, highly immunogenic, elicited
functional antibodies and T-cell responses. Overall, the data suggests that rhesus
of Indian and Chinese origins can be interchangeably used to compare the safety and
immunogenicity of next-generation of malaria vaccines and adjuvants.
AN - WOS:000501230000004
AU - Martin, M. L.
AU - Bitzer, A. A.
AU - Schrader, A.
AU - Bergmann-Leitner, E. S.
AU - Soto, K.
AU - Zou, X. Y.
AU - Beck, Z.
AU - Matyas, G. R.
AU - Dutta, S.
C7 - 377
DA - NOV 27
DO - 10.1186/s12936-019-3014-5
IS - 1
PY - 2019
SN - 1475-2875
ST - Comparison of immunogenicity and safety outcomes of a malaria vaccine
FMP013/ALFQ in rhesus macaques (Macaca mulatta) of Indian and Chinese origin
T2 - MALARIA JOURNAL
TI - Comparison of immunogenicity and safety outcomes of a malaria vaccine
FMP013/ALFQ in rhesus macaques (Macaca mulatta) of Indian and Chinese origin
VL - 18
ID - 6822
ER -
TY - JOUR
AB - Rabies is a lethal disease in humans and animals, killing approximately
60,000 people every year. Currently, there is no treatment available, except post-
exposure prophylaxis (PEP) that can be administered whenever exposure to a rabid
animal took place. Here we describe the beneficial effects of a combination
treatment initiated at day 4 post infection, containing anti-viral drugs and immune
modulators in infected mice. Combination therapy resulted in significant increase
in survival time (P < 0.05) and significantly lowers viral RNA in the brain and
spinal cord (P < 0.05). Furthermore, treatment influenced markers of pyroptosis and
apoptosis and early inflammatory response as measured by the levels of TNF-α.
Morphological lesions were absent in rabies virus infected mice with few signs of
inflammation. However, these were not significant between the different groups. ©
2018 The Authors
AU - Martina, B. E. E.
AU - Smreczak, M.
AU - Orlowska, A.
AU - Marzec, A.
AU - Trebas, P.
AU - Roose, J. M.
AU - Zmudzinski, J.
AU - Gerhauser, I.
AU - Wohlsein, P.
AU - Baumgärtner, W.
AU - Osterhaus, A. D. M. E.
AU - Koraka, P.
DB - Scopus
DO - 10.1016/j.vaccine.2018.05.065
IS - 33
KW - Blood–brain barrier
Combination treatment
Rabies
Therapy
Animals
Apoptosis
Brain
Cell Line, Tumor
Chiroptera
Female
Infliximab
Mannitol
Mice
Mice, Inbred C57BL
Post-Exposure Prophylaxis
Pyroptosis
RNA, Viral
Sorafenib
Spinal Cord
alpha interferon
antivirus agent
beta interferon
favipiravir
infliximab
ribavirin
sorafenib
virus RNA
mannitol
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
brain level
controlled study
female
inflammation
mouse
nonhuman
priority journal
pyroptosis
rabies
Rabies virus
silver haired bat Rabies virus
spinal cord
survival time
animal
bat
brain
C57BL mouse
genetics
metabolism
physiology
post exposure prophylaxis
tumor cell line
virology
M3 - Article
PY - 2019
SP - 4736-4742
ST - Combination drug treatment prolongs survival of experimentally infected mice
with silver-haired bat rabies virus
T2 - Vaccine
TI - Combination drug treatment prolongs survival of experimentally infected mice
with silver-haired bat rabies virus
85047387954&doi=10.1016%2fj.vaccine.2018.05.065&partnerID=40&md5=1aef87577aad6ff061
ec9e8916b5a989
VL - 37
ID - 5390
ER -
TY - JOUR
AB - Aim: Infections associated with medical devices are an important cause of
morbidity and mortality. Microorganisms are responsible for catheter infections
that may then result in the local or systemic dissemination of the microorganism
into the bloodstream. The aim of this study was to evaluate the antimicrobial
activity of silver nanoparticles (AgNPs) embedded in polyurethane plastics,
commonly used for catheter fabrication. Materials & methods: AgNPs in the range of
25-30 nm were synthesized and embedded in polyurethane plastics at different
concentrations. The antimicrobial activities of these plastics were tested against
the three pathogenic microorganisms, Escherichia coli, Staphylococcus epidermidis
and Candida albicans, frequently associated with catheter infections. The
cytotoxicity of the plastics was evaluated on human-derived macrophages using
propidium iodide and the secretion of the pro- and anti-inflammatory cytokines IL-
6, IL-10 and TNF-a was measured using ELISA. Results: A significant reduction of 6-
to 7-log in the number of bacteria was measured, while a reduction of 90% was
measured in the case of C. albicans. Neither cytotoxic effect on macrophages nor
immunological response was observed. Conclusion: Plastics embedded with AgNPs have
great potential to limit microbial colonization of implanted medical devices. ©
2013 Future Medicine Ltd.
AU - Martínez-Gutiérrez, F.
AU - Guajardo-Pacheco, J. M.
AU - Noriega-Trevino, M. E.
AU - Thi, E. P.
AU - Reiner, N.
AU - Orrantia, E.
AU - Av-Gay, Y.
AU - Ruiz, F.
AU - Bach, H.
DB - Scopus
DO - 10.2217/fmb.13.5
IS - 3
KW - antimicrobial activity
cytotoxicity
immunological response
medical devices
interleukin 10
interleukin 6
plastic
propidium iodide
silver nanoparticle
article
Candida albicans
Escherichia coli
human
human cell
macrophage
medical device
priority journal
M3 - Article
PY - 2013
SP - 403-411
ST - Antimicrobial activity, cytotoxicity and inflammatory response of novel
plastics embedded with silver nanoparticles
T2 - Future Microbiology
TI - Antimicrobial activity, cytotoxicity and inflammatory response of novel
plastics embedded with silver nanoparticles
84874751981&doi=10.2217%2ffmb.13.5&partnerID=40&md5=c3be556215ad71f0e47decf3ebfb5d3
5
VL - 8
ID - 5678
ER -
TY - JOUR
AB - The incorporation of nanoparticles (NPs) in industrial and biomedical
applications has increased significantly in recent years, yet their hazardous and
toxic effects have not been studied extensively. Here, we studied the effects of 24
nm silver NPs (AgNPs) on a panel of bacteria isolated from medical devices used in
a hospital intensive care unit. The cytotoxic effects were evaluated in macrophages
and the expression of the inflammatory cytokines IL-6, IL-10 and TNF-α were
quantified. The effects of NPs on coagulation were tested in vitro in plasma-based
assays. We demonstrated that 24 nm AgNPs were effective in suppressing the growth
of clinically relevant bacteria with moderate to high levels of antibiotic
resistance. The NPs had a moderate inhibitory effect when coagulation was initiated
through the intrinsic pathway. However, these NPs are cytotoxic to macrophages and
are able to elicit an inflammatory response. Thus, beneficial and potential harmful
effects of 24 nm AgNPs on biomedical devices must be weighed in further studies in
vivo. From the Clinical Editor: The authors of this study demonstrate that gallic
acid reduced 24 nm Ag NPs are effective in suppressing growth of clinically
relevant antibiotic resistant bacteria. However, these NPs also exhibit cytotoxic
properties to macrophages and may trigger an inflammatory response. Thus, the
balance of beneficial and potential harmful effects must be weighed carefully in
further studies. © 2012 Elsevier Inc.
AU - Martínez-Gutierrez, F.
AU - Thi, E. P.
AU - Silverman, J. M.
AU - de Oliveira, C. C.
AU - Svensson, S. L.
AU - Hoek, A. V.
AU - Sánchez, E. M.
AU - Reiner, N. E.
AU - Gaynor, E. C.
AU - Pryzdial, E. L. G.
AU - Conway, E. M.
AU - Orrantia, E.
AU - Ruiz, F.
AU - Av-Gay, Y.
AU - Bach, H.
DB - Scopus
DO - 10.1016/j.nano.2011.06.014
IS - 3
Clinical isolates
Coagulation effect
Immunological response
Bacteria
Blood Coagulation
Cell Death
Cell Line
Cytokines
Humans
Inflammation
Light
Macrophages
Metal Nanoparticles
Particle Size
Scattering, Radiation
Silver
Antibiotics
Coagulation
Cytotoxicity
Intensive care units
Nanoparticles
interleukin 10
interleukin 6
silver nanoparticle
Antibiotic resistance
Antibiotic-resistant bacteria
Biomedical devices
Cytotoxic
Cytotoxic effects
Cytotoxicity effects
Gallic acids
Harmful effects
In-vitro
In-vivo
Inhibitory effect
Medical Devices
Toxic effect
article
bacterial growth
bacterial strain
bacterium isolation
blood clotting
cytotoxicity
food poisoning
human
human cell
immune response
in vitro study
inflammation
intensive care
macrophage
medical device
nonhuman
protein expression
M3 - Article
PY - 2012
SP - 328-336
ST - Antibacterial activity, inflammatory response, coagulation and cytotoxicity
effects of silver nanoparticles
T2 - Nanomedicine: Nanotechnology, Biology, and Medicine
TI - Antibacterial activity, inflammatory response, coagulation and cytotoxicity
84858617840&doi=10.1016%2fj.nano.2011.06.014&partnerID=40&md5=d4dae78d02ac1d9f53598
38f2c6ab566
VL - 8
ID - 5686
ER -
TY - JOUR
AB - The incorporation of nanoparticles (NPs) in industrial and biomedical
applications has increased significantly in recent years, yet their hazardous and
toxic effects have not been studied extensively. Here, we studied the effects of 24
nm silver NPs (AgNPs) on a panel of bacteria isolated from medical devices used in
a hospital intensive care unit. The cytotoxic effects were evaluated in macrophages
and the expression of the inflammatory cytokines IL-6, IL-10 and TNF-alpha were
quantified. The effects of NPs on coagulation were tested in vitro in plasma-based
assays. We demonstrated that 24 nm AgNPs were effective in suppressing the growth
of clinically relevant bacteria with moderate to high levels of antibiotic
resistance. The NPs had a moderate inhibitory effect when coagulation was initiated
through the intrinsic pathway. However, these NPs are cytotoxic to macrophages and
are able to elicit an inflammatory response. Thus, beneficial and potential harmful
effects of 24 nm AgNPs on biomedical devices must be weighed in further studies in
vivo.
AN - WOS:000301949300008
AU - Martinez-Gutierrez, F.
AU - Thi, E. P.
AU - Silverman, J. M.
AU - de Oliveira, C. C.
AU - Svensson, S. L.
AU - Hoek, A. V.
AU - Sanchez, E. M.
AU - Reiner, N. E.
AU - Gaynor, E. C.
AU - Pryzdial, E. L. G.
AU - Conway, E. M.
AU - Orrantia, E.
AU - Ruiz, F.
AU - Av-Gay, Y.
AU - Bach, H.
DA - APR
DO - 10.1016/j.nano.2011.06.014
IS - 3
PY - 2012
SN - 1549-9634
1549-9642
SP - 328-336
ST - Antibacterial activity, inflammatory response, coagulation and cytotoxicity
TI - Antibacterial activity, inflammatory response, coagulation and cytotoxicity
VL - 8
ID - 5916
ER -
TY - JOUR
AB - Biomaterials are often used in orthopedic surgery like cavity fillings.
However, related complications often require long-term systemic antibiotics, device
removal, and extended rehabilitation. Hydroxyapatite/silver (HA/Ag) composites have
been proposed as implantation biomaterials owing to the osteogenic properties of
hydroxyapatite and to the antimicrobial efficiency of silver. Nevertheless, higher
silver concentrations induce cytotoxic effects. The aim of this study was to
synthesize and characterize HA/Ag nanocomposites that will allow us to use lower
concentrations of silver nanoparticles with better antimicrobial efficiency and
anti-inflammatory properties. The characterization of HA/Ag was performed by
scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction,
Fourier-transform infrared spectra, X-ray photoelectron spectroscopy, and laser
diffraction. Bioactivity was evaluated under a simulated body fluid. The viability
of osteoblast like-cells (MG-63) was determined by MTT (3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyl-2H-tetrazolium bromide) and the antimicrobial activity was
evaluated by the standard McFarland method. The detection of nitric oxide was
measured by a colorimetric assay and the inflammatory cytokines by flow cytometry.
We obtained particulate composites of calcium phosphates identified as
hydroxyapatite and silver nanoparticles. The bioactivity of the HA/Ag
nanocomposites on SFB was confirmed by apatite formations. The viability of MG-63
cells was not affected. We also found antimicrobial activity against Escherichia
coli, Staphylococcus aureus, and Candida albicans owing to the presence of silver
nanoparticles at non-cytotoxic concentrations. HA/Ag reduced the release of nitric
oxide and decreased the secretion of IL-1 and TNF-α in cells stimulated with
Lipopolysaccharide (LPS). In conclusion, the inflammatory and antimicrobial
capacity of the HA/Ag nanocomposites, as well as its bioactivity and low
cytotoxicity make it a candidate as an implantation biomaterial for bone tissues
engineering and clinical practices in orthopedic, oral and maxillofacial surgery. ©
The Author(s) 2019.
AU - Martínez-Sanmiguel, J. J.
AU - G Zarate-Triviño, D.
AU - Hernandez-Delgadillo, R.
AU - Giraldo-Betancur, A. L.
AU - Pineda-Aguilar, N.
AU - Galindo-Rodríguez, S. A.
AU - Franco-Molina, M. A.
AU - Hernández-Martínez, S. P.
AU - Rodríguez-Padilla, C.
DB - Scopus
DO - 10.1177/0885328219835995
IS - 10
biomaterial
composite materials
Nitric oxide
tissue engineering and bone regeneration
Bone Regeneration
Candida albicans
Candidiasis
Cell Line
Durapatite
Escherichia coli
Humans
Nanocomposites
Silver
Biocompatibility
Biomaterials
Bone
Calcium phosphate
Composite materials
Hydroxyapatite
Metal nanoparticles
Phosphate minerals
Photoelectron spectroscopy
Silver compounds
Surgery
Tissue
Tissue regeneration
calcium phosphate
hydroxyapatite
interleukin 1
lipopolysaccharide
nitric oxide
silver nanoparticle
antiinfective agent
nanocomposite
silver
Bone regeneration
Fourier transform infrared spectra
Oral and maxillofacial surgeries
Osteoblast-like cells
Particulate composites
Release of nitric oxide
Article
cell viability
controlled study
cytokine release
cytotoxicity
MG-63 cell line
nonhuman
osteoblast
particle size
powder
priority journal
synthesis
bacterial infection
bone regeneration
candidiasis
cell line
chemistry
drug effect
human
ultrastructure
Bioactivity
M3 - Article
PY - 2019
SP - 1314-1326
ST - Anti-inflammatory and antimicrobial activity of bioactive
hydroxyapatite/silver nanocomposites
T2 - Journal of Biomaterials Applications
TI - Anti-inflammatory and antimicrobial activity of bioactive
85066163675&doi=10.1177%2f0885328219835995&partnerID=40&md5=b57e1e95ad12e1db7234161
4b617f941
VL - 33
ID - 5400
ER -
TY - JOUR
AB - Biomaterials are often used in orthopedic surgery like cavity fillings.
However, related complications often require long-term systemic antibiotics, device
removal, and extended rehabilitation. Hydroxyapatite/silver (HA/Ag) composites have
been proposed as implantation biomaterials owing to the osteogenic properties of
hydroxyapatite and to the antimicrobial efficiency of silver. Nevertheless, higher
silver concentrations induce cytotoxic effects. The aim of this study was to
synthesize and characterize HA/Ag nanocomposites that will allow us to use lower
concentrations of silver nanoparticles with better antimicrobial efficiency and
anti-inflammatory properties. The characterization of HA/Ag was performed by
scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction,
Fourier-transform infrared spectra, X-ray photoelectron spectroscopy, and laser
diffraction. Bioactivity was evaluated under a simulated body fluid. The viability
of osteoblast like-cells (MG-63) was determined by MTT (3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyl-2H-tetrazolium bromide) and the antimicrobial activity was
evaluated by the standard McFarland method. The detection of nitric oxide was
measured by a colorimetric assay and the inflammatory cytokines by flow cytometry.
We obtained particulate composites of calcium phosphates identified as
hydroxyapatite and silver nanoparticles. The bioactivity of the HA/Ag
nanocomposites on SFB was confirmed by apatite formations. The viability of MG-63
cells was not affected. We also found antimicrobial activity against Escherichia
coli, Staphylococcus aureus, and Candida albicans owing to the presence of silver
nanoparticles at non-cytotoxic concentrations. HA/Ag reduced the release of nitric
oxide and decreased the secretion of IL-1 and TNF-alpha in cells stimulated with
Lipopolysaccharide (LPS). In conclusion, the inflammatory and antimicrobial
capacity of the HA/Ag nanocomposites, as well as its bioactivity and low
cytotoxicity make it a candidate as an implantation biomaterial for bone tissues
engineering and clinical practices in orthopedic, oral and maxillofacial surgery.
AN - WOS:000470861400002
AU - Martinez-Sanmiguel, J. J.
AU - Zarate-Trivino, D. G.
AU - Hernandez-Delgadillo, R.
AU - Giraldo-Betancur, A. L.
AU - Pineda-Aguilar, N.
AU - Galindo-Rodriguez, S. A.
AU - Franco-Molina, M. A.
AU - Hernandez-Martinez, S. P.
AU - Rodriguez-Padilla, C.
DA - MAY
DO - 10.1177/0885328219835995
IS - 10
PY - 2019
SN - 0885-3282
1530-8022
SP - 1314-1326
ST - Anti-inflammatory and antimicrobial activity of bioactive
T2 - JOURNAL OF BIOMATERIALS APPLICATIONS
TI - Anti-inflammatory and antimicrobial activity of bioactive
VL - 33
ID - 5873
ER -
TY - JOUR
AB - ABSTRACT BACKGROUND: Serotonin (5-HT) is present in the epithelial
enterochromaffin cells (EC), mast cells of the lamina propria and enteric neurons.
The 5-HT is involved in regulating motility, secretion, gut sensation, immune
system and inflammation. OBJECTIVE: Evaluate the effects of diabetes and quercetin
supplementation on serotoninergic cells and its cell loss by apoptosis in jejunal
mucosa of streptozotocin-induced diabetic rats (STZ-rats). METHODS: Twenty-four
male Wistar rats were divided into four groups: normoglycemic (C), normoglycemic
supplemented with 40 mg/day quercetin (Q), diabetic (D) and diabetic supplemented
with 40 mg/day quercetin (DQ). After 120 days, the jejunum was collected and
fixated in Zamboni's solution for 18 h. After obtaining cryosections,
immunohistochemistry was performed to label 5-HT and caspase-3. Quantification of
5-HT and caspase-3 immunoreactive (IR) cells in the lamina propria, villi and
crypts were performed. RESULTS: The diabetic condition displayed an increase of the
number of 5-HT-IR cells in villi and crypts, while decreased number of these cells
was observed in lamina propria in the jejunum of STZ-rats. In the diabetic animals,
an increased density of apoptotic cells in epithelial villi and crypts of the
jejunum was observed, whereas a decreased number of caspase-3-IR cells was observed
in lamina propria. Possibly, quercetin supplementation slightly suppressed the
apoptosis phenomena in the epithelial villi and crypts of the STZ-rats, however the
opposite effect was observed on the 5-HT-IR cells of the lamina propria. Quercetin
supplementation on healthy animals promoted few changes of serotoninergic function
and apoptotic stimuli. CONCLUSION: These results suggest that quercetin
supplementation mostly improved the serotonergic function affected by diabetes
maybe due to antioxidant and anti-inflammatory properties of quercetin.
RESUMO CONTEXTO: A serotonina (5-HT) está presente nas células epiteliais
enterocromafins (CE), nos mastócitos da lâmina própria e nos neurônios entéricos. A
5-HT está envolvida na regulação da motilidade, secreção, nocepção intestinal,
sistema imunológico e inflamação. Objetivo: Avaliar os efeitos do diabetes e da
suplementação de quercetina sobre a função serotoninérgica e a perda celular por
apoptose na mucosa jejunal de ratos diabéticos induzidos por estreptozotocina
(ratos STZ). MÉTODOS: Vinte e quatro ratos Wistar machos foram divididos em quatro
grupos: normoglicêmico (C), normoglicêmico suplementado com quercetina 40 mg/dia
(Q), diabético (D) e diabético suplementado com quercetina 40 mg/dia (DQ). Após 120
dias, o jejuno foi coletado e fixado na solução de Zamboni por 18 horas. Após a
obtenção de cortes em criostato, a imuno-histoquímica foi realizada para marcar 5-
HT e caspase-3. A quantificação de células imunorreativas (IR) à 5-HT e caspase-3
foram realizadas na lâmina própria, vilosidades e criptas. RESULTADOS: A condição
diabética ocasionou um aumento do número de células 5-HT-IR nas vilosidades e
criptas, enquanto que na lâmina própria houve uma redução dessas células, no jejuno
de ratos STZ. Nos animais diabéticos, foi observada uma densidade aumentada de
células apoptóticas no epitélio do jejuno, tanto nas vilosidades quanto nas
criptas, por outro lado um número reduzido de células caspase-3-IR foi observado na
lâmina própria. Possivelmente, a suplementação de quercetina suprimiu ligeiramente
os fenômenos de apoptose no epitélio de vilosidades e criptas do jejuno de ratos
STZ, no entanto, o efeito oposto foi observado nas células 5-HT-IR da lâmina
própria. A suplementação com quercetina em animais saudáveis promoveu poucas
alterações na função serotoninérgica e nos estímulos apoptóticos. CONCLUSÃO: Estes
resultados sugerem que a suplementação de quercetina melhorou principalmente a
função serotoninérgica afetada pelo diabetes, talvez devido às propriedades
antioxidantes e anti-inflamatórias da quercetina.
AD - Martins-Perles, Juliana Vanessa Colombo
Universidade Estadual de Maringá. Departamento de Ciências Morfológicas.
Laboratório de Plasticidade Neural Entérica. Maringá. BR
Zignani, Isabela
Souza, Sara Raquel Garcia de
Frez, Flávia Cristina Vieira
Bossolani, Gleison Daion Piovezana
Zanoni, Jacqueline Nelisis
AU - Martins-Perles, Juliana Vanessa Colombo
AU - Zignani, Isabela
AU - Souza, Sara Raquel Garcia de
AU - Frez, Flávia Cristina Vieira
AU - Bossolani, Gleison Daion Piovezana
AU - Zanoni, Jacqueline Nelisis
C1 - 20200325
DA - 2019/10
DB - LILACS
DO - 10.1590/s0004-2803.201900000-81
IS - 4
KW - Apoptose
Apoptosis
Diabetes mellitus
Intestinal mucosa
Mucosa intestinal
Quercetin
Quercetina
Ratos
Rats
Serotonin
Serotonina
LA - en
PY - 2019
SN - 0004-2803
SP - 405-411
ST - A suplementação com quercetina previne mudanças em células imunorreativas à
serotonina e caspase-3 do jejuno de ratos diabéticos
T2 - Arq. gastroenterol
TI - A suplementação com quercetina previne mudanças em células imunorreativas à
serotonina e caspase-3 do jejuno de ratos diabéticos
TT - Quercetin supplementation prevents changes in the serotonin and caspase-3
immunoreactive cells of the jejunum of diabetic rats
28032019000400405
VL - 56
ID - 4921
ER -
TY - JOUR
AB - Interaction of nanoparticles with food matrix components may cause
unpredictable health complications. Using an improved Caco-2 cell-based in vitro
(co-)culture model the potential of quercetin as one of the major food flavonoids
to alter the effect of silver nanoparticles (Ag-NPs) <20 nm in the human intestinal
mucosa at real life concentrations was investigated. Ag-NPs (15-90 μg/ml) decreased
cell viability and reduced thiol groups, induced oxidative/nitrosative stress and
lipid peroxidation and led to activity changes of various antioxidant enzymes after
3 h exposure. The contribution of Ag+ ions within the concentrations released from
nanoparticles was shown to be less important, compared to Ag-NPs. While leading to
inflammatory response in the intestines, Ag-NPs, paradoxically, also showed a
potential anti-infammatory effect manifested in down-regulated IL-8 levels.
Quercetin, co-administered with Ag-NPs, led to a reduction of cytotoxicity,
oxidative stress, and recovered metabolic activity of Caco-2 cells, suggesting the
protective effects of this flavonoid against the harmful effect of Ag-NPs.
Quercetin not only alleviated the effect of Ag-NPs on the gastrointestinal cells,
but also demonstrated a potential to serve as a tool for reversible modulation of
intestinal permeability. © 2016.
AU - Martirosyan, A.
AU - Grintzalis, K.
AU - Polet, M.
AU - Laloux, L.
DB - Scopus
DO - 10.1016/j.toxlet.2016.04.018
KW - Gastrointestinal tract
Oxidative and nitrosative stress
Quercetin
Caco-2 Cells
Cell Survival
Cytoprotection
Humans
Inflammation
Intestinal Mucosa
Lipid Peroxidation
Metal Nanoparticles
Oxidative Stress
Reactive Nitrogen Species
Risk Assessment
Silver Compounds
acid phosphatase
catalase
interleukin 6
interleukin 8
quercetin
silver nanoparticle
superoxide
thiol group
metal nanoparticle
silver derivative
aerobic metabolism
Article
CACO 2 cell line
cell viability
coculture
comparative study
controlled study
cytokine release
cytotoxicity
down regulation
enzyme activity
human
human cell
human tissue
in vitro study
intestine mucosa
lipid peroxidation
MTT assay
nitrosative stress
oxidative stress
physical chemistry
priority journal
protein expression
X ray analysis
Caco-2 cell line
cell protection
cell survival
chemically induced
dose response
drug effects
inflammation
metabolism
pathology
risk assessment
M3 - Article
PY - 2016
SP - 36-45
ST - Tuning the inflammatory response to silver nanoparticles via quercetin in
Caco-2 (co-)cultures as model of the human intestinal mucosa
T2 - Toxicology Letters
TI - Tuning the inflammatory response to silver nanoparticles via quercetin in
Caco-2 (co-)cultures as model of the human intestinal mucosa
84973130811&doi=10.1016%2fj.toxlet.2016.04.018&partnerID=40&md5=70a109d29297f7679ae
76bb4cd3b73f2
VL - 253
ID - 5477
ER -
TY - JOUR
AB - In this contribution, the great potential of surface enhanced Raman
spectroscopy (SERS) in a lab-on-a-chip (LOC) device for the detection of analyte
molecules in a complex environment is demonstrated. Using LOC-SERS, the enzyme
activity of thiopurine S-methyltransferase (TPMT) is analysed and identified in
lysed red blood cells. The conversion of 6-mercaptopurine to 6-methylmercaptopurine
catalysed by TPMT is observed as it gives evidence for the enzyme activity. Being
able to determine the TPMT activity before starting a treatment using 6-
mercaptopurine, an optimized dosage can be applied to each patient and serious
toxicity appearing within thiopurine treatment will be prevented.
AN - WOS:000291037800009
AU - Marz, A.
AU - Monch, B.
AU - Rosch, P.
AU - Kiehntopf, M.
AU - Henkel, T.
AU - Popp, J.
DA - JUL
DO - 10.1007/s00216-011-4811-z
IS - 9
PY - 2011
SN - 1618-2642
SP - 2755-2761
ST - Detection of thiopurine methyltransferase activity in lysed red blood cells
by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)
T2 - ANALYTICAL AND BIOANALYTICAL CHEMISTRY
TI - Detection of thiopurine methyltransferase activity in lysed red blood cells
by means of lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS)
VL - 400
ID - 6760
ER -
TY - JOUR
AB - An endothelial cell growth-suppressing factor (EGSF) was purified from the
serum-free conditioned medium of the mouse P388D1 culture in the presence of
carboxymethylated curdlan. The purified EGSF showed two bands corresponding to the
molecular masses of 55 and 63 kDa by silver staining on a SDS-polyacrylamide gel
under reducing conditions. This factor strongly suppressed the proliferation of
endothelial cells from bovine artery, human umbilical vein, and human dermal vas
capillare and this suppression was observed to be reversible. We found that EGSF
was a potent chemoattractant for macrophages and neutrophils. EGSF mediated the
adhesion of neutrophils to BAEs and transendothelial migration of neutrophils.
Macrophages stimulated by EGSF produced nitrite in a dose-dependent manner. EGSF
did not affect the proliferation of T lymphocytes. These findings suggest that EGSF
acts not only as a potent inhibitor for the growth of endothelial cells but also an
activator for macrophages and neutrophils. Thus EGSF plays a role in an
inflammatory response in the endothelium.
AN - WOS:000082142000014
AU - Matsunaga, T.
AU - Usui, S.
AU - Ukai, S.
AU - Kiho, T.
AU - Hirano, K.
DA - JUL
DO - 10.1271/bbb.63.1228
IS - 7
PY - 1999
SN - 0916-8451
1347-6947
SP - 1228-1237
ST - Activation of macrophages and neutrophils by an endothelium growth
suppressing factor
T2 - BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
TI - Activation of macrophages and neutrophils by an endothelium growth
suppressing factor
VL - 63
ID - 6697
ER -
TY - JOUR
AB - INTRODUCTION: Administration of the NO inhibitor Nwð-nitro-L-arginine methyl
ester (NAME) and a high-salt diet (HS) promotes severe albuminuria and renal
injury, which regresses upon discontinuation of treatments. OBJECTIVE: We
investigated whether these changes reappear after reinstitution of HS, and whether
they are prevented by treatment with the antilymphocyte agent mycophenolate mofetil
(MMF) or the AT-1 receptor blocker losartan (L). Adult male Munich-Wistar rats
received NAME and HS. A control Group (C) received only HS. After 20 days, rats
receiving HS and NAME exhibited severe hypertension and albuminuria. After a 30-day
recovery period, hypertension was attenuated and albuminuria had virtually
disappeared. MATERIAL AND METHODS: Rats were then distributed among the following
groups: HS, receiving HS; NS, receiving a normal salt (NS) diet; HS-MMF, receiving
HS and MMF; HS-LOS, receiving HS and L; HS-HDZ, receiving HS and hydralazine (HDZ).
Sixty days later, NS rats showed only slight albuminuria and renal damage or
inflammation. In contrast, HS rats developed severe hypertension, marked
glomerulosclerosis with interstitial expansion and renal infiltration by
macrophages and angiotensin II-positive cells. The group treated with losartan had
lowered blood pressure and a lack of albuminuria or renal injury. MMF provided
similar protection without altering blood pressure, suggesting a nonhemodynamic
effect, a hypothesis reinforced by the finding that HDZ lowered blood pressure
without preventing renal injury. RESULTS: These results indicate that treatment
with HS and NAME predisposes to the development of hypertension and renal injury
upon salt overload, characterizing a new model of chronic nephropathy. CONCLUSION:
The response to MMF or L, but not HDZ, suggests a key role for inflammatory rather
than hemodynamic factors.
INTRODUÇÃO: A administração de Nômega-nitro-L-arginina metiléster (NAME), um
inibidor da produção de NO, com dieta rica em sal (HS) promove albuminúria e dano
renal graves, reversíveis ao interromperem-se os tratamentos. OBJETIVO:
Investigamos se tais alterações recrudescem ao reinstituir-se a HS e se são
prevenidas pelo micofenolato mofetil (MMF), um agente antilinfócito, ou losartan,
um bloqueador do receptor AT-1. MATERIAL E MÉTODOS: Ratos Münich-Wistar machos
adultos receberam NAME e HS. Um grupo controle (C) recebeu apenas HS. Após 20 dias,
os ratos que receberam HS e NAME exibiam hipertensão e albuminúria graves. Após
recuperação de 30 dias, a hipertensão atenuou-se e a albuminúria praticamente
desapareceu. Formaram-se então os grupos: HS, recebendo HS; NS, recebendo dieta
normal em sal (NS); HS-MMF, recebendo HS e MMF; HS-LOS, recebendo HS e losartan;
HS-HDZ, recebendo HS e hidralazina. Após sessenta dias os ratos NS tinham
albuminúria e dano/inflamação renal apenas discretos. Já os ratos HS desenvolveram
hipertensão e glomerulosclerose acentuadas, expansão intersticial e infiltração
renal por macrófagos e células positivas para angiotensina II. Losartan baixou a
pressão arterial e preveniu albuminúria e lesão renal. MMF proporcionou proteção
semelhante sem alteração pressórica, sugerindo a ação de mecanismos não
hemodinâmicos, hipótese reforçada pelo achado de que a HDZ baixou a pressão
arterial sem prevenir a nefropatia. RESULTADOS: Esses resultados indicam que o
tratamento com HS e NAME predispõe ao desenvolvimento de hipertensão e lesão renal
induzidos por excesso de sal, caracterizando um novo modelo de nefropatia crônica.
CONCLUSÃO: A resposta ao MMF ou losartan, mas não à hidralazina, sugere o
predomínio de fatores inflamatórios.
AD - Mattar, Ana Lúcia
Universidade de São Paulo. Faculdade de Medicina. Department of Clinical Medicine.
Renal Division. São Paulo. BR
Machado, Flávia Gomes
Fujihara, Clarice Kazue
Malheiros, Denise Maria Avancini Costa
Zatz, Roberto
AU - Mattar, Ana Lúcia
AU - Machado, Flávia Gomes
AU - Fujihara, Clarice Kazue
AU - Malheiros, Denise Maria Avancini Costa
AU - Zatz, Roberto
C1 - 20080122
DA - 2007/00
DB - LILACS
IS - 6
KW - AT-1 receptor blocker
AT-1 receptor bloqueador
Angiotensin II
Angiotensina
Chronic kidney disease
Doença crônica do rim
Excesso de sal
Micofenolato mofetil
Mycophenolate mofetil
Salt overload
LA - en
PY - 2007
SN - 1807-5932
SP - 749-756
ST - Persistent hypertension and progressive renal injury induced by salt overload
after short term nitric oxide inhibition
T2 - Clinics
TI - Persistent hypertension and progressive renal injury induced by salt overload
after short term nitric oxide inhibition
59322007000600015
VL - 62
ID - 4955
ER -
TY - JOUR
AB - The skin, acting as the outer protection of the human body, is most
vulnerable to injury. Wound healing can often be impaired, leading to chronic,
hard-to-heal wounds. For this reason, searching for the most effective dressings
that can significantly enhance the wound healing process is necessary. In this
regard, silk fibroin, a protein derived from silk fibres that has excellent
properties, is noteworthy. Silk fibroin is highly biocompatible and biodegradable.
It can easily make various dressings, which can be loaded with additional
substances to improve healing. Dressings based on silk fibroin have anti-
inflammatory, pro-angiogenic properties and significantly accelerate skin wound
healing, even compared to commercially available wound dressings. Animal studies
confirm the beneficial influence of silk fibroin in wound healing. Clinical
research focusing on fibroin dressings is also promising. These properties make
silk fibroin a remarkable natural material for creating innovative, simple, and
effective dressings for skin wound healing. In this review, we summarise the
application of silk fibroin biomaterials as wound dressings in full-thickness,
burn, and diabetic wounds in preclinical and clinical settings. © 2022 by the
authors.
AU - Mazurek, Ł
AU - Szudzik, M.
AU - Rybka, M.
AU - Konop, M.
C7 - 1852
DB - Scopus
DO - 10.3390/biom12121852
IS - 12
KW - burn wounds
diabetic wounds
silk biomaterials
silk fibroin
skin wounds
wound healing
biomaterial
colony stimulating factor 1
hydrogel
protein kinase B
silver nanoparticle
somatomedin C
biocompatibility
burn
cell proliferation
diabetes mellitus
diabetic wound
epithelization
histology
human
neutrophil
nonhuman
preclinical study
Review
skin injury
systematic review
thickness
M3 - Review
PY - 2022
ST - Silk Fibroin Biomaterials and Their Beneficial Role in Skin Wound Healing
T2 - Biomolecules
TI - Silk Fibroin Biomaterials and Their Beneficial Role in Skin Wound Healing
85144692159&doi=10.3390%2fbiom12121852&partnerID=40&md5=077a060394c1ca0642004526f2f
488ed
VL - 12
ID - 5044
ER -
TY - JOUR
AB - Background: Acute systemic inflammatory response syndrome arising from
infection can lead to multiple organ failure and death, with greater susceptibility
occurring in immunocompromised individuals. Moreover, sub-acute chronic
inflammation is a contributor to the pathology of diverse degenerative diseases
(Parkinson's disease, Alzheimer's disease and arthritis). Given the known
limitations in Western medicine to treat a broad range of inflammatory related
illness as well as the emergence of antibiotic resistance, there is a renewed
interest in complementary and alternative medicines (CAMs) to achieve these means.
Methods: A high throughput (HTP) screening of > 1400 commonly sold natural products
(bulk herbs, cooking spices, teas, leaves, supplement components, nutraceutical
food components, fruit and vegetables, rinds, seeds, polyphenolics etc.) was
conducted to elucidate anti-inflammatory substances in lipopolysaccharide (LPS) (E.
coli serotype O111: B4) monocytes: RAW 264.7 macrophages [peripheral], BV-2
microglia [brain]) relative to hydrocortisone, dexamethasone and L-N6-(1Iminoethyl)
lysine (L-NIL). HTP evaluation was also carried out for lethal kill curves against
E. coli 0157: H7 1x10(6) CFU/mL relative to penicillin. Validation studies were
performed to assess cytokine profiling using antibody arrays. Findings were
corroborated by independent ELISAs and NO2-/iNOS expression quantified using the
Griess Reagent and immunocytochemistry, respectively. For robust screening, we
developed an in-vitro efficacy paradigm to ensure anti-inflammatory parameters were
observed independent of cytotoxicity. This caution was taken given that many plants
exert tumoricidal and anti-inflammatory effects at close range through similar
signaling pathways, which could lead to false positives. Results: The data show
that activated BV-2microglia cells (+ LPS 1 mu g/ml) release > 10-fold greater IL-
6, MIP1/2, RANTES and nitric oxide (NO2-), where RAW 264.7 macrophages (+ LPS 1 mu
g/ml) produced > 10-fold rise in sTNFR2, MCP-1, IL-6, GCSF, RANTES and NO2-. Data
validation studies establish hydrocortisone and dexamethasone as suppressing
multiple pro-inflammatory processes, where L-NIL suppressed NO2-, but had no effect
on iNOS expression or IL-6. The screening results demonstrate relative few valid
hits with anti-inflammatory effects at < 250 mu g/ml for the following: Bay Leaf
(Laurus nobilis), Elecampagne Root (Inula helenium), Tansy (Tanacetum vulgare),
Yerba (Eriodictyon californicum) and Centipeda (Centipeda minima), Ashwagandha
(Withania somnifera), Feverfew (Tanacetum parthenium), Rosemary (Rosmarinus
officinalis), Turmeric Root (Curcuma Longa), Osha Root (Ligusticum porteri), Green
Tea (Camellia sinensis) and constituents: cardamonin, apigenin, quercetin,
biochanin A, eupatorin, (-)-epigallocatechin gallate (EGCG) and butein. Natural
products lethal against [E. coli 0157: H7] where the LC50 < 100 mu g/ml included
bioactive silver hydrosol-Argentyn 23, green tea (its constituents EGCG >
Polyphenon 60 > (-)-Gallocatechin > Epicatechin > (+)-Catechin), Grapeseed Extract
(Vitis vinifera), Chinese Gallnut (its constituents gallic acid > caffeic acid) and
gallic acid containing plants such as Babul Chall Bark (Acacia Arabica), Arjun
(Terminalia Arjuna) and Bayberry Root Bark (Morella Cerifera). Conclusions: These
findings emphasize and validate the previous work of others and identify the most
effective CAM anti-inflammatory, antibacterial compounds using these models. Future
work will be required to evaluate potential combination strategies for long-term
use to prevent chronic inflammation and possibly lower the risk of sepsis in
immunocompromised at risk populations.
AN - WOS:000388339200003
AU - Mazzio, E. A.
AU - Li, N.
AU - Bauer, D.
AU - Mendonca, P.
AU - Taka, E.
AU - Darb, M.
AU - Thomas, L.
AU - Williams, H.
AU - Soliman, K. F. A.
C7 - 467
DA - NOV 15
DO - 10.1186/s12906-016-1429-x
PY - 2016
SN - 1472-6882
ST - Natural product HTP screening for antibacterial (E-coli 0157: H7) and anti-
inflammatory agents in (LPS from E-coli O111: B4) activated macrophages and
microglial cells; focus on sepsis
T2 - BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
TI - Natural product HTP screening for antibacterial (E-coli 0157: H7) and anti-
inflammatory agents in (LPS from E-coli O111: B4) activated macrophages and
VL - 16
ID - 6253
ER -
TY - JOUR
AB - Background: Acute systemic inflammatory response syndrome arising from
infection can lead to multiple organ failure and death, with greater susceptibility
occurring in immunocompromised individuals. Moreover, sub-acute chronic
inflammation is a contributor to the pathology of diverse degenerative diseases
(Parkinson's disease, Alzheimer's disease and arthritis). Given the known
limitations in Western medicine to treat a broad range of inflammatory related
illness as well as the emergence of antibiotic resistance, there is a renewed
interest in complementary and alternative medicines (CAMs) to achieve these means.
Methods: A high throughput (HTP) screening of >1400 commonly sold natural products
(bulk herbs, cooking spices, teas, leaves, supplement components, nutraceutical
food components, fruit and vegetables, rinds, seeds, polyphenolics etc.) was
conducted to elucidate anti-inflammatory substances in lipopolysaccharide (LPS) (E.
coli serotype O111:B4) monocytes: RAW 264.7 macrophages [peripheral], BV-2
microglia [brain]) relative to hydrocortisone, dexamethasone and L-N6-
(1Iminoethyl)lysine (L-NIL). HTP evaluation was also carried out for lethal kill
curves against E.coli 0157:H7 1x106 CFU/mL relative to penicillin. Validation
studies were performed to assess cytokine profiling using antibody arrays. Findings
were corroborated by independent ELISAs and NO2-/iNOS expression quantified using
the Griess Reagent and immunocytochemistry, respectively. For robust screening, we
developed an in-vitro efficacy paradigm to ensure anti-inflammatory parameters were
observed independent of cytotoxicity. This caution was taken given that many plants
exert tumoricidal and anti-inflammatory effects at close range through similar
signaling pathways, which could lead to false positives. Results: The data show
that activated BV-2 microglia cells (+ LPS 1μg/ml) release >10-fold greater IL-6,
MIP1/2, RANTES and nitric oxide (NO2-), where RAW 264.7 macrophages (+ LPS 1μg/ml)
produced > 10-fold rise in sTNFR2, MCP-1, IL-6, GCSF, RANTES and NO2- Data
validation studies establish hydrocortisone and dexamethasone as suppressing
multiple pro-inflammatory processes, where L-NIL suppressed NO2-, but had no effect
on iNOS expression or IL-6. The screening results demonstrate relative few valid
hits with anti-inflammatory effects at < 250μg/ml for the following: Bay Leaf
(Laurus nobilis), Elecampagne Root (Inula helenium), Tansy (Tanacetum
vulgare),Yerba (Eriodictyon californicum) and Centipeda (Centipeda minima),
Ashwagandha (Withania somnifera), Feverfew (Tanacetum parthenium), Rosemary
(Rosmarinus officinalis), Turmeric Root (Curcuma Longa), Osha Root (Ligusticum
porteri), Green Tea (Camellia sinensis) and constituents: cardamonin, apigenin,
quercetin, biochanin A, eupatorin, (-)-epigallocatechin gallate (EGCG) and butein.
Natural products lethal against [E. coli 0157:H7] where the LC50 < 100 μg/ml
included bioactive silver hydrosol-Argentyn 23, green tea (its constituents EGCG >
Polyphenon 60 > (-)-Gallocatechin > Epicatechin > (+)-Catechin), Grapeseed Extract
(Vitis vinifera), Chinese Gallnut (its constituents gallic acid > caffeic acid) and
gallic acid containing plants such as Babul Chall Bark (Acacia Arabica), Arjun
(Terminalia Arjuna) and Bayberry Root Bark (Morella Cerifera). Conclusions: These
findings emphasize and validate the previous work of others and identify the most
effective CAM anti-inflammatory, antibacterial compounds using these models. Future
work will be required to evaluate potential combination strategies for long-term
use to prevent chronic inflammation and possibly lower the risk of sepsis in
immunocompromised at risk populations. © 2016 The Author(s).
AU - Mazzio, E. A.
AU - Li, N.
AU - Bauer, D.
AU - Mendonca, P.
AU - Taka, E.
AU - Darb, M.
AU - Thomas, L.
AU - Williams, H.
AU - Soliman, K. F. A.
C7 - 467
DB - Scopus
DO - 10.1186/s12906-016-1429-x
IS - 1
KW - Animals
Biological Products
Drug Evaluation, Preclinical
Escherichia coli
High-Throughput Screening Assays
Humans
Lipopolysaccharides
Macrophages
Mice
Microglia
RAW 264.7 Cells
Sepsis
antiinfective agent
apicidin
apigenin
biochanin A
butein
cardamonin
Curcuma longa extract
curcumin
dexamethasone
Emblica officinalis extract
eupatorin
hydrocortisone
interleukin 6
Laurus nobilis extract
nitric oxide
plant extract
quercetin
RANTES
Tanacetum parthenium extract
tumor necrosis factor receptor associated factor 2
unclassified drug
unindexed drug
Withania somnifera extract
biological product
lipopolysaccharide
Acacia
animal cell
antibody screening
Article
Camellia sinensis
Centipeda minima
controlled study
Curcuma longa
cytotoxicity
Eriodictyon
grape
immunocytochemistry
Inula helenium
Laurus nobilis
Ligusticum porteri
medicinal plant
microglia
Morella Cerifera
mouse
nonhuman
protein analysis
protein expression
protein synthesis
rosemary
sepsis
Tanacetum parthenium
Tanacetum vulgare
Terminalia arjuna
Withania somnifera
animal
drug effects
human
immunology
macrophage
microbiology
preclinical study
RAW 264.7 cell line
M3 - Article
PY - 2016
ST - Natural product HTP screening for antibacterial (E.coli 0157:H7) and anti-
inflammatory agents in (LPS from E. coli O111:B4) activated macrophages and
TI - Natural product HTP screening for antibacterial (E.coli 0157:H7) and anti-
inflammatory agents in (LPS from E. coli O111:B4) activated macrophages and
84996537965&doi=10.1186%2fs12906-016-1429-
x&partnerID=40&md5=7142452c608bef1005e313ef5da892d7
VL - 16
ID - 5554
ER -
TY - JOUR
AB - During the 1991 GulfWar, U.S. service members were exposed to depleted
uranium (DU) through friendly-fire incidents involving DU munitions and vehicles
protected by DU armor. Routes of exposure to DU involved inhalation of soluble and
insoluble DU oxide particles, wound contamination, and retained embedded DU metal
fragments that continue to oxidize in situ and release DU to the systemic
circulation. A biennial health surveillance program established for this group of
Veterans by the U. S. Department of Veterans Affairs has shown continuously
elevated urine DU concentrations in the subset of veterans with embedded fragments
for over 20 years. While the 2011 assessment was comprehensive, few clinically
significant U-related health effects were observed. This report is focused on
health outcomes associated with two primary target organs of concern for long term
effects of this combat-related exposure to DU. Renal biomarkers showed minimal DU-
related effects on proximal tubule function and cytotoxicity, but significant
biomarker results were observed when urine concentrations of multiple metals also
found in fragments were examined together. Pulmonary tests and questionnaire
results indicate that pulmonary function after 20 y remains within the clinical
normal range. Imaging of DU embedded fragment-associated tissue for signs of
inflammatory or proliferative reactions possibly associated with foreign body
transformation or with local alpha emissions from DU was also conducted using PET-
CT and ultrasound. These imaging tools may be helpful in guiding decisions
regarding removal of fragments. Health Phys. 104(4):347Y361; 2013
AN - WOS:000315601100001
AU - McDiarmid, M. A.
AU - Gaitens, J. M.
AU - Hines, S.
AU - Breyer, R.
AU - Wong-You-Cheong, J. J.
AU - Engelhardt, S. M.
AU - Oliver, M.
AU - Gucer, P.
AU - Kane, R.
AU - Cernich, A.
AU - Kaup, B.
AU - Hoover, D.
AU - Gaspari, A. A.
AU - Liu, J.
AU - Harberts, E.
AU - Brown, L.
AU - Centeno, J. A.
AU - Gray, P. J.
AU - Xu, H. N.
AU - Squibb, K. S.
DA - APR
DO - 10.1097/HP.0b013e31827b1740
IS - 4
PY - 2013
SN - 0017-9078
1538-5159
SP - 347-361
ST - THE GULF WAR DEPLETED URANIUM COHORT AT 20 YEARS: BIOASSAY RESULTS AND NOVEL
APPROACHES TO FRAGMENT SURVEILLANCE
T2 - HEALTH PHYSICS
TI - THE GULF WAR DEPLETED URANIUM COHORT AT 20 YEARS: BIOASSAY RESULTS AND NOVEL
APPROACHES TO FRAGMENT SURVEILLANCE
VL - 104
ID - 6818
ER -
TY - JOUR
AB - Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been
associated with type IV Ni-induced hypersensitivity. We hypothesised that the full-
thickness human-derived oral mucosa model employed for biocompatibility testing of
base-metal dental alloys would provide insights into the mechanisms of Ni-induced
toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto
Alloderm™ and maintained until full thickness was achieved prior to Ni-Cr and
cobalt-chromium (Co-Cr) alloy disc exposure (2-72 h). Biocompatibility assessment
involved histological analyses with cell viability measurements, oxidative stress
responses, inflammatory cytokine expression and cellular toxicity analyses.
Inductively coupled plasma mass spectrometry analysis determined elemental ion
release levels. We detected adverse morphology with significant reductions in cell
viability, significant increases in oxidative stress, inflammatory cytokine
expression and cellular toxicity for the Ni-Cr alloy-treated oral mucosal models
compared with untreated oral mucosal models, and adverse effects were increased for
the Ni-Cr alloy that leached the most Ni. Co-Cr demonstrated significantly enhanced
biocompatibility compared with Ni-Cr alloy-treated oral mucosal models. The human-
derived full-thickness oral mucosal model discriminated between dental alloys and
provided insights into the mechanisms of Ni-induced toxicity, highlighting
potential clinical relevance. © 2011 Acta Materialia Inc. Published by Elsevier
Ltd. All rights reserved.
AU - McGinley, E. L.
AU - Moran, G. P.
AU - Fleming, G. J. P.
DB - Scopus
DO - 10.1016/j.actbio.2011.08.017
IS - 1
Dental casting alloys
ICP-MS
Inflammation
Oxidative stress
Binary alloys
Cell culture
Cobalt alloys
Cytotoxicity
Dental alloys
Inductively coupled plasma mass spectrometry
Nickel alloys
Silver alloys
chromium
cobalt
cytokine
nickel
Base metals
Biocompatibility testing
Cellular toxicities
Cytokine expression
Fixed prosthodontics
Keratinocytes
Nickel-Chromium alloys
Oral mucosa
article
bare metal stent
biocompatibility
cell viability
controlled study
cytotoxicity
denture
fibroblast
histology
human
human tissue
keratinocyte
mass spectrometry
mouth mucosa
normal human
oxidative stress
priority journal
protein expression
semen analysis
Chromium alloys
M3 - Article
PY - 2012
SP - 432-438
ST - Base-metal dental casting alloy biocompatibility assessment using a human-
derived three-dimensional oral mucosal model
TI - Base-metal dental casting alloy biocompatibility assessment using a human-
derived three-dimensional oral mucosal model
80255130971&doi=10.1016%2fj.actbio.2011.08.017&partnerID=40&md5=a1d1ec499eecb7031b1
a199308b4e9a4
VL - 8
ID - 5709
ER -
TY - JOUR
AB - History shows that metal-based drugs and remedies have been known and used
since very ancient times. For example, silver was employed in the treatment of
wounds and ulcers according to the Greek physician Hippocrates, but its
antimicrobial properties had probably been recognized long before because was used
to make vessels for storing liquids in pure form. The ancient Egyptians also knew
how to sterilize water with copper. The medical use of gold can be dated back to
2500 B.C. in China. However, the new era of metal-based medicine started almost 50
years ago when cisplatin was shown to inhibit cellular division in Escherichia
coli, thereby leading to the first studies of its antitumor activity in rats and
its assessment as one of the most powerful drugs for use against different types of
cancer, although many other novel metal-based drugs are promising and they are
attracting growing attention in modern clinical medicine. Gold salts and arsenic
compounds have been in use for decades in the treatment of rheumatoid arthritis and
syphilis, respectively, but studies of cisplatin have definitely shifted the
attention of researchers to the pool of transition "heavy" metals as potential
therapeutic agents. Rhodium, iridium, palladium, osmium, and the other so-called
noble elements have been the subjects of intensive investigations, thereby leading
to the production of a series of complex compounds with remarkable anticancer
activities, as well as antirheumatic, antimalarial, and antimicrobial drugs. The
number of published studies in this field is huge and they have already been the
subjects of careful review. In this review, we provide a detailed account of the
latest results (2010-2013) and their potential uses in the cure of severe diseases.
AN - WOS:000348016700014
AU - Medici, S.
AU - Peana, M.
AU - Nurchi, V. M.
AU - Lachowicz, J. I.
AU - Crisponi, G.
AU - Zoroddu, M. A.
DA - FEB 1
DO - 10.1016/j.ccr.2014.08.002
PY - 2015
SN - 0010-8545
1873-3840
SP - 329-350
ST - Noble metals in medicine: Latest advances
T2 - COORDINATION CHEMISTRY REVIEWS
TI - Noble metals in medicine: Latest advances
VL - 284
ID - 6583
ER -
TY - CONF
AB - Adipose tissue derived stem cells (ASCs) has applications in soft tissue
replacement-based tissue engineering. ASCs can potentially reduce many of the
disadvantages of autologous fat transplantation such as donor-site morbidity and
immune system rejection. Although, ASCs hold clinical relevance as a potential cell
therapy candidate, widespread use of them is hampered due to inadequate data on the
fate of stem cells after transplant. Hence a method to facilitate long term
tracking of the cells will enable better understanding of stem cell fate in stem
cell-based therapeutics. Here, we employ biocompatible surface functionalized
nanorods for tracking the adipogenesis and osteogenesis differentiation of ASCs.
Anisotropic plasmonic nanostructures based on silver (Ag) and gold (Au) have
received much attention owing to their tunable size and shape dependent localized
surface plasmon resonance (LSPR) with multiple applications such as biological
contrast agents, photothermal conversion, plasmon-enhanced spectroscopies, optical
sensors and in catalysis. Hyperspectral microscopy combining both nanoscale imaging
and spectral characteristics from plasmonic nanostructures provides a powerful tool
for their identification and quantitative spectral analysis of plasmonic
nanostructures with unprecedented level of details. Here, we present the analysis
of single particle spectroscopy of gold nanorods and their orientation dependent
scattering properties using hyperspectral microscopy and validated with correlated
high-resolution electron microscopy. Fairly monodisperse gold nanorods with bright
longitudinal SPR centered at about 663 nm were synthesized using bromide-free
surfactant mixture consisting of cetyltrimethylammonium chloride and sodium oleate.
The nanorods were successfully characterized by UV-Visible spectroscopy, DLS, XPS,
and TEM results. Dark-field hyperspectral and second harmonic generation (SHG)
microscopy were performed on individual gold nanorods and their optical scattering
spectra were analyzed for imaging orientation of single nanorods. The initial
results revealed scattering spectra from individual gold nanorods displayed
measurable spectral-shifts from their collective LSPR spectrum from bulk
measurements performed using UV-Visible spectroscopy. The analysis and utility of
gold nanorods for labeling stem cells and the orientation dependent spectral
features of nanorods inside the cells will be characterized and discussed in
detail. The cell viability, differentiation capacity, gene expression, potential
cytotoxicity due to nanorods such as inflammatory molecule and reactive oxygen
species production, adipogenic and osteogenic potential will be evaluated using
histochemical staining and quantitative polymerase chain reaction (qPCR). The study
has implications towards tracking individual nanorods in complex biological systems
and beyond. © 2019 SPIE.
AU - Mehta, N.
AU - Sahu, S.
AU - Shaik, S.
AU - Hasan, S. M.
AU - Devireddy, R.
AU - Gartia, M. R.
DB - Scopus
DO - 10.1117/12.2510836
KW - Hyperspectral
Nanofabrication
Nanomedicine
Nanostructure
Orientation
Plasmonic
Spectral response
Stem cells
Biocompatibility
Biomolecules
Cell engineering
Chlorine compounds
Crystal orientation
Cytology
Gene expression
Gold
Harmonic generation
High resolution electron microscopy
Histology
Infrared spectrophotometers
Nanorods
Nanostructures
Nanotechnology
Nonlinear optics
Plasmonics
Plasmons
Spectrum analysis
Surface plasmon resonance
Tissue
Tissue engineering
Adipose tissuederived stem cell (ASCs)
Cetyltrimethylammonium chloride
HyperSpectral
Localized surface plasmon resonance
Quantitative polymerase chain reaction
Second harmonic generation microscopies (SHG)
Hyperspectral imaging
PY - 2019
ST - Dark-field hyperspectral imaging of single plasmonic gold nanorods and their
scattering characteristics in complex biological environments
T2 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
TI - Dark-field hyperspectral imaging of single plasmonic gold nanorods and their
scattering characteristics in complex biological environments
85065404180&doi=10.1117%2f12.2510836&partnerID=40&md5=369284518d06aaf136c4351e58057
e6e
VL - 10881
ID - 5409
ER -
TY - CPAPER
AB - Adipose tissue derived stem cells (ASCs) has applications in soft tissue
replacement-based tissue engineering. ASCs can potentially reduce many of the
disadvantages of autologous fat transplantation such as donor-site morbidity and
immune system rejection. Although, ASCs hold clinical relevance as a potential cell
therapy candidate, widespread use of them is hampered due to inadequate data on the
fate of stem cells after transplant. Hence a method to facilitate long term
tracking of the cells will enable better understanding of stem cell fate in stem
cell-based therapeutics. Here, we employ biocompatible surface functionalized
nanorods for tracking the adipogenesis and osteogenesis differentiation of ASCs.
Anisotropic plasmonic nanostructures based on silver (Ag) and gold (Au) have
received much attention owing to their tunable size and shape dependent localized
surface plasmon resonance (LSPR) with multiple applications such as biological
contrast agents, photothermal conversion, plasmon-enhanced spectroscopies, optical
sensors and in catalysis. Hyperspectral microscopy combining both nanoscale imaging
and spectral characteristics from plasmonic nanostructures provides a powerful tool
for their identification and quantitative spectral analysis of plasmonic
nanostructures with unprecedented level of details. Here, we present the analysis
of single particle spectroscopy of gold nanorods and their orientation dependent
scattering properties using hyperspectral microscopy and validated with correlated
high-resolution electron microscopy. Fairly monodisperse gold nanorods with bright
longitudinal SPR centered at about 663 nm were synthesized using bromide-free
surfactant mixture consisting of cetyltrimethylammonium chloride and sodium oleate.
The nanorods were successfully characterized by UV-Visible spectroscopy, DLS, XPS,
and TEM results. Dark-field hyperspectral and second harmonic generation (SHG)
microscopy were performed on individual gold nanorods and their optical scattering
spectra were analyzed for imaging orientation of single nanorods. The initial
results revealed scattering spectra from individual gold nanorods displayed
measurable spectral-shifts from their collective LSPR spectrum from bulk
measurements performed using UV-Visible spectroscopy. The analysis and utility of
gold nanorods for labeling stem cells and the orientation dependent spectral
features of nanorods inside the cells will be characterized and discussed in
detail. The cell viability, differentiation capacity, gene expression, potential
cytotoxicity due to nanorods such as inflammatory molecule and reactive oxygen
species production, adipogenic and osteogenic potential will be evaluated using
histochemical staining and quantitative polymerase chain reaction (qPCR). The study
has implications towards tracking individual nanorods in complex biological systems
and beyond.
AN - WOS:000473083400023
AU - Mehta, N.
AU - Sahu, S.
AU - Shaik, S.
AU - Hasan, S. M.
AU - Devireddy, R.
AU - Gartia, M. R.
DO - 10.1117/12.2510836
PY - 2019
T2 - IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES XVII
TI - Dark-field Hyperspectral Imaging of Single Plasmonic Gold Nanorods and Their
Scattering Characteristics in Complex Biological Environments
VL - 10881
ID - 6549
ER -
TY - JOUR
AB - Heavy metals have different adverse impacts on different life stages of fish
species with attempts to use natural antioxidants to counteract their effects. So,
the present study investigated the potential protective effects of Amphora
coffeaeformis extract against arsenic-induced hemato-biochemical alterations in
African catfish, Clarias gariepinus. The fish exposed to sub-lethal concentrations
of arsenic; 19.2 and 38.3 mg/L (1/8 and 1/4 of 96h-LC50 value, 153.17 mg/L) for 15
days. The main effect of arsenic was recorded in some blood parameters such as
RBC's count, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and
white blood cells. As for biochemical parameters, the main effect of arsenic was
significant for alkaline phosphatase, glucose, uric acid, creatinine, albumin,
globulin, and albumin/globulin. Also, the residue of arsenic in fish muscles showed
significant effects. The majority of these arsenic-induced parameters were improved
with dietary supplements of the diatom A. coffeaeformis. So, Amphora extract can be
used as detoxification factor on fishes induced by arsenic due to its biologically
active components providing protections like antioxidant, antiviral, antibacterial,
and anti-inflammatory. Besides, they have excellent contents of proteins and
carbohydrates which are supposed to enhance the effect of these compounds.
AN - WOS:000522310700001
AU - Mekkawy, I. A.
AU - Mahmoud, U. M.
AU - Moneeb, R. H.
AU - Sayed, Aedh
C7 - 191
DA - MAR 31
DO - 10.3389/fmars.2020.00191
PY - 2020
SN - 2296-7745
ST - Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato-
Biochemical Alterations of African Catfish (Clarias gariepinus)
T2 - FRONTIERS IN MARINE SCIENCE
TI - Significance Assessment of Amphora coffeaeformis in Arsenic-Induced Hemato-
Biochemical Alterations of African Catfish (Clarias gariepinus)
VL - 7
ID - 6737
ER -
TY - JOUR
AB - Metal and metal oxide nanoparticles (NPs) are promising antibacterial agents.
They have a broad antimicrobial activity against both Gram-positive and Gram-
negative bacteria, viruses, and protozoans. The use of NPs reduces the possibility
of the microbial resistance development. This review briefly shows the general
mechanisms and the main factors of antibacterial activity of NPs. In this article,
a comprehensive review of the recent researches in the field of new antimicrobial
agents with superior long-term bactericidal activity and low toxicity is provided.
The review gives the examples of synthesis of double and triple nanocomposites
based on following oxides: CuO, ZnO, Fe3O4, Ag2O, MnO2, etc. including metal and
nonmetal doped nanocomposites (for example with Ag, Ce, Cr, Mn, Nd, Co, Sn, Fe, N,
F, etc.). Compared with bactericidal action of individual oxides, the
nanocomposites demonstrate superior antibacterial activity and have synergistic
effects. For example, the antimicrobial activity of ZnO against both Gram-positive
and Gram-negative bacteria was increased by -100% by formation of triple
nanocomposites ZnO-MnO2-Cu2O or ZnO-Ag2O-Ag2S. Similar effect was showed for Ce-
doped ZnO and Zn-doped CuO. The present article also provides the examples of
nanocomposites containing NPs and organic (chitosan, cellulose,
polyvinylpyrrolidone, biopolymers, etc.) or inorganic materials with special
structure (graphene oxide, TiO2 nanotubes, silica) which demonstrate controlled
release and longterm antibacterial activity. All of the considered nanocomposites
and their combinations have a pronounced long-term antimicrobial effect including
against antibiotic-resistant strains. They are able to prevent the formation of
microbial biofilms on biotic and abiotic surfaces, have low toxicity to eukaryotic
cells, demonstrate anti-inflammatory and woundhealing properties in compositions
with polymers (sodium alginate, collagen, polyvinylpyrrolidone, etc.). The use of
nanoscale systems can solve several important practical problems at the same time:
saving of long-term antimicrobial activities while reducing the number of
compounds, creation of new antimicrobial agents with low toxicity and reduced
environmental impact, development of new biocidal materials, including new coatings
for effective antimicrobial protection of medical devices.
AN - WOS:000594651400003
AU - Meleshko, A. A.
AU - Afinogenova, A. G.
AU - Afinogenov, G. E.
AU - Spiridonova, A. A.
AU - Tolstoy, V. P.
DA - OCT-DEC
DO - 10.15789/2220-7619-AIA-1512
IS - 4
PY - 2020
SN - 2220-7619
2313-7398
SP - 639-654
ST - ANTIBACTERIAL INORGANIC AGENTS: EFFICIENCY OF USING MULTICOMPONENT SYSTEMS
T2 - INFEKTSIYA I IMMUNITET
TI - ANTIBACTERIAL INORGANIC AGENTS: EFFICIENCY OF USING MULTICOMPONENT SYSTEMS
VL - 10
ID - 6690
ER -
TY - JOUR
AB - The secretome of human amniotic fluid stem cells (AFSCs) has great potential
as a therapeutic agent in regenerative medicine. However, it must be produced in a
clinically compliant manner before it can be used in humans. In this study, we
developed a means of producing a biologically active secretome from AFSCs that is
free of all exogenous molecules. We demonstrate that the full secretome is capable
of promoting stem cell proliferation, migration, and protection of cells against
senescence. Furthermore, it has significant anti-inflammatory properties. Most
importantly, we show that it promotes tissue regeneration in a model of muscle
damage. We then demonstrate that the secretome contains extracellular vesicles
(EVs) that harbor much, but not all, of the biological activity of the whole
secretome. Proteomic characterization of the EV and free secretome fraction shows
the presence of numerous molecules specific to each fraction that could be key
regulators of tissue regeneration. Intriguingly, we show that the EVs only contain
miRNA and not mRNA. This suggests that tissue regeneration in the host is mediated
by the action of EVs modifying existing, rather than imposing new, signaling
pathways. The EVs harbor significant anti-inflammatory activity as well as promote
angiogenesis, the latter may be the mechanistic explanation for their ability to
promote muscle regeneration after cardiotoxin injury. © Copyright 2017, Mary Ann
Liebert, Inc. 2017.
AU - Mellows, B.
AU - Mitchell, R.
AU - Antonioli, M.
AU - Kretz, O.
AU - Chambers, D.
AU - Zeuner, M. T.
AU - Denecke, B.
AU - Musante, L.
AU - Ramachandra, D. L.
AU - Debacq-Chainiaux, F.
AU - Holthofer, H.
AU - Joch, B.
AU - Ray, S.
AU - Widera, D.
AU - David, A. L.
AU - Huber, T. B.
AU - Dengjel, J.
AU - De Coppi, P.
AU - Patel, K.
DB - Scopus
DO - 10.1089/scd.2017.0089
IS - 18
KW - miRNA
muscle
regeneration
secretome
Amniotic Fluid
Animals
Cell Line
Cells, Cultured
Embryonic Stem Cells
Extracellular Vesicles
Humans
Male
Mice
Mice, Inbred C57BL
MicroRNAs
Muscle, Skeletal
Proteome
Regeneration
ABCE1 protein
ABCF1 protein
ABR protein
AP2A1 protein
beta galactosidase
C73 protein
CD34 antigen
CD45 antigen
CYFIP1 protein
CYFIP2 protein
DNM1 protein
DNM2 protein
DNM3 protein
heat shock protein
Hermes antigen
hexokinase 1
histone deacetylase 1
histone deacetylase 2
luciferase
microRNA
microRNA 125b 5p
microRNA 1273 3p
microRNA 3196
microRNA 762
myosin heavy chain
nucleic acid
protein
Thy 1 antigen
unclassified drug
proteome
acute disease
adipogenesis
amniotic fluid stem cell
angiogenesis
animal experiment
animal model
apoptosis
Article
autophagy
biological activity
bone development
capillary density
cell count
cell function
cell migration
cell proliferation
cell protection
cell stress
chondrogenesis
controlled study
enzyme activation
enzyme activity
exosome
female
human
human cell
in vivo study
male
mouse
muscle injury
muscle regeneration
nonhuman
priority journal
senescence
signal transduction
silver staining
amnion fluid
animal
C57BL mouse
cell culture
cell line
cytology
embryonic stem cell
genetics
metabolism
physiology
skeletal muscle
transplantation
M3 - Article
PY - 2017
SP - 1316-1333
ST - Protein and Molecular Characterization of a Clinically Compliant Amniotic
Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating
Muscle Regeneration Through Enhancement of Angiogenesis
T2 - Stem Cells and Development
TI - Protein and Molecular Characterization of a Clinically Compliant Amniotic
Fluid Stem Cell-Derived Extracellular Vesicle Fraction Capable of Accelerating
Muscle Regeneration Through Enhancement of Angiogenesis
85029223185&doi=10.1089%2fscd.2017.0089&partnerID=40&md5=41235926ea1391cb02b6f6f148
c57ed2
VL - 26
ID - 5492
ER -
TY - CONF
AB - The aim of this study was to assess whether thermal trauma induced oxidative
stress altered the balance between oxidant and antioxidant systems in the blood of
burn wound rats in the absence and presence of silver nanoparticles and S-
nitrosoglutathione, GSNO. Free silver nanoparticles, free GSNO and silver
nanoparticles + GSNO had no cytotoxic effects. Under anesthesia, the shaved dorsum
of the rats was exposed to 90°C (burn group) water bath. Studied compounds were
administered topically immediately and at 28 days after the burn injury, four times
a day. Silver nanoparticles and silver nanoparticles + GSNO were no toxic in vitro
and in vivo. There were no significant differences in the levels of urea,
creatinine, aminotransferases and hematological parameters, in control-burn groups
(free silver nanoparticles) and treated-burn groups (free GSNO or silver
nanoparticles + GSNO). There were no differences in lipid peroxidation and in the
levels of protein carbonyls and glutathione, used as oxidative stress markers. A
little inflammatory cell response, papillary dermis vascularization, fibroblasts
differentiated into contractile myofibroblasts and the presence of a large amount
of extracellular matrix were evidenced in treated groups following skin injury.
These results indicate that silver nanoparticles and GSNO may provide an effective
action on wound healing.
AU - Melo, P. S.
AU - Marcato, P. D.
AU - Huber, S. C.
AU - Ferreira, I. R.
AU - Paula, L. B. De
AU - Almeida, A. B. A.
AU - Durán, N.
AU - Torsoni, S.
AU - Seabra, A. B.
AU - Alves, O. L.
DB - Scopus
DO - 10.1088/1742-6596/304/1/012027
ET - 1
KW - Cell culture
Cytotoxicity
Fibroblasts
Metal nanoparticles
Nanoparticles
Oxidative stress
Rats
Tissue
Urea
Antioxidant systems
Hematological parameters
Inflammatory cells
Lipid peroxidation
Oxidative stress markers
S-nitrosoglutathione
Silver
PY - 2011
ST - Nanoparticles in treatment of thermal injured rats: Is it safe?
T2 - Journal of Physics: Conference Series
TI - Nanoparticles in treatment of thermal injured rats: Is it safe?
80052049054&doi=10.1088%2f1742-
6596%2f304%2f1%2f012027&partnerID=40&md5=de624b3394e778bf782221d5a40e8e53
VL - 304
ID - 5747
ER -
TY - JOUR
AB - The experimental use of poly (alcohol-vinyl) (PVA) as a skin curative is
increasing widely. However, the use of this hydrogel is challenging due to its
favorable properties for microbiota growth. The association with silver
nanoparticles (AgNPs) as an antimicrobial agent turns the match for PVA as a
dressing, as it focuses on creating a physical barrier to avoid wound dehydration.
When associated with extracellular components, such as the collagen matrix, the
device obtained can create the desired biological conditions to act as a skin
substitute. This study aimed to analyze the anti-microbiological activity and the
in vitro and in vivo responses of a bilaminar device of PVA containing AgNPs
associated with a membrane of collagen-hyaluronic acid (col-HA). Additionally,
mesenchymal stem cells were cultured in the device to evaluate in vitro responses
and in vivo immunomodulatory and healing behavior. The device morphology revealed a
porous pattern that favored water retention and in vitro cell adhesion. Controlled
wounds in the dorsal back of rat skins revealed a striking skin remodeling with new
epidermis fulfilling all previously injured areas after 14 and 28 days. No
infections or significant inflammations were observed, despite increased
angiogenesis, and no fibrosis-markers were identified as compared to controls.
Although few antibacterial activities were obtained, the addition of AgNPs
prevented fungal growth. All results demonstrated that the combination of the
components used here as a dermal device, chosen according to previous miscellany
studies of low/mid-cost biomaterials, can promote skin protection avoiding
infections and dehydration, minimize the typical wound inflammatory responses, and
favor the cellular healing responses, features that give rise to further clinical
trials of the device here developed
AN - WOS:000665448100001
AU - Mendes, D.
AU - Hausen, M. A.
AU - Asami, J.
AU - Higa, A. M.
AU - Leite, F. L.
AU - Mambrini, G. P.
AU - Rossi, A. L.
AU - Komatsu, D.
AU - Duek, E. A. D.
C7 - 742
DA - JUN
IS - 6
PY - 2021
SN - 2079-6382
ST - A New Dermal Substitute Containing Polyvinyl Alcohol with Silver
Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches
T2 - ANTIBIOTICS-BASEL
TI - A New Dermal Substitute Containing Polyvinyl Alcohol with Silver
Nanoparticles and Collagen with Hyaluronic Acid: In Vitro and In Vivo Approaches
VL - 10
ID - 6101
ER -
TY - JOUR
AB - In the present study, the effects of 10- or 100-nm silica oxide (SiO2) NPs on
human peripheral blood mononuclear cells (PBMC) were examined. Cytotoxic effects
and oxidative stress effects, including glutathione (GSH) depletion, the formation
of protein radical species, and proinflammatory cytokine responses, were measured.
PBMC exposed to 10-nm NP concentrations from 50 to 4,000 ppm showed concentration-
response increases in cell death; whereas, for 100-nm NPs, PBMC viability was not
lost at <500 ppm. Interestingly, 10-nm NPs were more cytotoxic and induced more
oxidative stress than 100-nm NPs. Immunoelectron micrographs show the cellular
distribution of GSH and NPs. As expected based on the viability data, the 10-nm NPs
disturbed cell morphology to a greater extent than did the 100-nm NPs. Antibody to
the radical scavenger, 5, 5-dimethyl-1-pyrroline N-oxide (DMPO), was used for
Western blot analysis of proteins with radicals; more DMPO proteins were found
after exposure to 10-nmNPs than 100-nm NPs. Examination of cytokines (TNF-alpha,
IL-1ra, IL-6, IL-8, IL-1 beta, and IFN-gamma) indicated that different ratios of
cytokines were expressed and released after exposure to 10- and 100-nm NPs. IL-1
beta production was enhanced by 10- and 100-nm NPs;, the cytotoxicity of the NPs
was associated with an increase in the IL-1 beta/IL-6 ratio and 100-nm NPs at
concentrations that did not induce loss of cell viability enhanced IL-1 beta and
IL-6 to an extent similar to phytohemagglutinin (PHA), a T cell mitogen. In
conclusion, our results indicate that SiO2 NPs trigger a cytokine inflammatory
response and induce oxidative stress in vitro, and NPs of the same chemistry, but
of different sizes, demonstrate differences in their intracellular distribution and
immunomodulatory properties, especially with regard to IL-1 beta and IL-6
expression.
AN - WOS:000344873100004
AU - Mendoza, A.
AU - Torres-Hernandez, J. A.
AU - Ault, J. G.
AU - Pedersen-Lane, J. H.
AU - Gao, D. H.
AU - Lawrence, D. A.
DA - NOV
DO - 10.1007/s12192-014-0502-y
IS - 6
PY - 2014
SN - 1355-8145
1466-1268
SP - 777-790
ST - Silica nanoparticles induce oxidative stress and inflammation of human
peripheral blood mononuclear cells
T2 - CELL STRESS & CHAPERONES
TI - Silica nanoparticles induce oxidative stress and inflammation of human
VL - 19
ID - 6519
ER -
TY - JOUR
AB - The ability of pathogenic bacteria to develop resistance mechanisms to avoid
the antimicrobial potential of antibiotics has become an increasing problem for the
healthcare system. The search for more effective and selective antimicrobial
materials, though not harmful to mammalian cells, seems imperative. Herein we
propose the use of gold-chitosan nanocomposites as effective bactericidal materials
avoiding damage to human cells. Nanocomposites were obtained by taking advantage of
the reductive and stabilizing action of chitosan solutions on two different gold
precursor concentrations. The resulting nanocomposites were added at different
final concentrations to a coculture model formed by Gram-positive (Staphylococcus
aureus) or Gram-negative (Escherichia coli) bacteria and human macrophages. Gold
chitosan colloids exhibited superior bactericidal ability against both bacterial
models without showing cytotoxicity on human cells at the concentrations tested.
Morphological and in vitro viability studies supported the feasibility of the
infection model here described to test novel bactericidal nanomaterials. Flow
cytometry and scanning electron microscopy analyses pointed to the disruption of
the bacterial wall as the lethal mechanism. Data obtained in the present study
suggest that gold chitosan nanocomposites are powerful and promising nanomaterials
for reducing bacteria associated infections, respecting the integrity of mammalian
cells, and displaying high selectivity against the studied bacteria.
AN - WOS:000402691600004
AU - Mendoza, G.
AU - Regiel-Futyra, A.
AU - Andreu, V.
AU - Sebastian, V.
AU - Kyziol, A.
AU - Stochel, G.
AU - Arruebo, M.
DA - MAY 31
DO - 10.1021/acsami.6b15123
IS - 21
PY - 2017
SN - 1944-8244
1944-8252
SP - 17693-17701
ST - Bactericidal Effect of Gold-Chitosan Nanocomposites in Coculture Models of
Pathogenic Bacteria and Human Macrophages
TI - Bactericidal Effect of Gold-Chitosan Nanocomposites in Coculture Models of
Pathogenic Bacteria and Human Macrophages
VL - 9
ID - 6777
ER -
TY - JOUR
AB - Nanotechnology is emerging as a promising modality for cancer treatment;
however, in the realm of cancer prevention, its full utility has yet to be
determined. Here, we discuss the potential of integrating nanotechnology in cancer
prevention to augment early diagnosis, precision targeting, and controlled release
of chemopreventive agents, reduced toxicity, risk/response assessment, and
personalized point-of-care monitoring. Cancer is a multistep, progressive disease;
the functional and acquired characteristics of the early precancer phenotype are
intrinsically different from those of a more advanced anaplastic or invasive
malignancy. Therefore, applying nanotechnology to precancers is likely to be far
more challenging than applying it to established disease. Frank cancers are more
readily identifiable through imaging and biomarker and histopathologic assessment
than their precancerous precursors. In addition, prevention subjects routinely have
more rigorous intervention criteria than therapy subjects. Any nanopreventive agent
developed to prevent sporadic cancers found in the general population must exhibit
a very low risk of serious side effects. In contrast, a greater risk of side
effects might be more acceptable in subjects at high risk for cancer. Using
nanotechnology to prevent cancer is an aspirational goal, but clearly identifying
the intermediate objectives and potential barriers is an essential first step in
this exciting journey. ©2014 American Association for Cancer Research.
AU - Menter, D. G.
AU - Patterson, S. L.
AU - Logsdon, C. D.
AU - Kopetz, S.
AU - Sood, A. K.
AU - Hawk, E. T.
DB - Scopus
DO - 10.1158/1940-6207.CAPR-14-0079
IS - 10
KW - Anti-Inflammatory Agents
Chemoprevention
Humans
Immune System
Liposomes
Nanomedicine
Nanotechnology
Neoplasms
Phenotype
Risk Assessment
RNA Interference
antiinfective agent
asparaginase macrogol
biological marker
celecoxib
chitosan
cisplatin
cytarabine
daunorubicin
doxorubicin
drug carrier
fluorouracil
gold nanoparticle
ingenol mebutate
liposome
nanotube
nucleotide
paclitaxel
polymer
prostate specific membrane antigen
quantum dot
raloxifene
silicon dioxide
silver nanoparticle
tamoxifen
unindexed drug
Wart virus vaccine
biomaterial
brain tumor
breast metastasis
cancer prevention
cancer risk
cancer therapy
chemoprophylaxis
diagnostic accuracy
drug response
early diagnosis
histopathology
human
immunomodulation
nanoencapsulation
nanomedicine
nanotechnology
non small cell lung cancer
nonhuman
particle size
phenotype
precancer
Review
risk assessment
surface charge
chemistry
immune system
procedures
RNA interference
M3 - Review
PY - 2014
SP - 973-992
ST - Convergence of nanotechnology and cancer prevention: Are we there yet?
T2 - Cancer Prevention Research
TI - Convergence of nanotechnology and cancer prevention: Are we there yet?
84907526151&doi=10.1158%2f1940-6207.CAPR-14-
0079&partnerID=40&md5=295985f10c4409ea84cf94503f6f0348
VL - 7
ID - 5591
ER -
TY - JOUR
AB - Psoriasis is a chronic Th1/Th17 lymphocytes-mediated inflammatory skin
disease, in which epidermal keratinocytes exhibit a peculiar senescent state,
resistance to apoptosis and the acquisition of senescence-associated secretory
phenotype (SASP). SASP consists of the release of soluble factors, including
IGFBPs, that exert extracellular and intracellular functions in IGF-dependent or
independent manner. In this report, we investigated the expression and function of
IGFBP2 in senescent keratinocytes isolated from the skin of patients with plaque
psoriasis. We found that IGFBP2 is aberrantly expressed and released by these cells
in vivo, as well as in vitro in keratinocyte cultures undergoing progressive
senescence, and it associates with the cyclin-dependent kinase inhibitors p21 and
p16 expression. For the first time, we provide evidence for a dual action of IGFBP2
in psoriatic keratinocytes during growth and senescence processes. While
extracellular IGFBP2 counter-regulates IGF-induced keratinocyte hyper-
proliferation, intracellular IGFBP2 inhibits apoptosis by interacting with p21 and
protecting it from ubiquitin-dependent degradation. Indeed, we found that
cytoplasmic p21 sustains anti-apoptotic processes, by inhibiting pro-caspase 3
cleavage and JNK phosphorylation in senescent psoriatic keratinocytes. As a
consequence, abrogation of p21, as well as that of IGFBP2, found to stabilize
cytoplasmic p21 levels, lead to the restoration of apoptosis mechanisms in
psoriatic keratinocytes, commonly observed in healthy cells.
AN - WOS:000530887200022
AU - Mercurio, L.
AU - Lulli, D.
AU - Mascia, F.
AU - Dellambra, E.
AU - Scarponi, C.
AU - Morelli, M.
AU - Valente, C.
AU - Carbone, M. L.
AU - Pallotta, S.
AU - Girolomoni, G.
AU - Albanesi, C.
AU - Pastore, S.
AU - Madonna, S.
DA - APR 30
DO - 10.18632/aging.103045
IS - 8
PY - 2020
SN - 1945-4589
SP - 6823-6851
ST - Intracellular Insulin-like growth factor binding protein 2 (IGFBP2)
contributes to the senescence of keratinocytes in psoriasis by stabilizing
cytoplasmic p21
T2 - AGING-US
TI - Intracellular Insulin-like growth factor binding protein 2 (IGFBP2)
contributes to the senescence of keratinocytes in psoriasis by stabilizing
cytoplasmic p21
VL - 12
ID - 6761
ER -
TY - JOUR
AB - Apoptosis is a permanent and dynamic physiological process by which an
organism eliminates the undesirable cells without causing an inflammatory response.
The objective of this work was to study the expression of FAS, DR4 and other
members of the TNF-R1 superfamily extrinsic route apoptotic receptors the DNA
fragmentation and the cellular apoptosis in placental samples at the early, mid and
late pregnancy on +/- 30, +/- 55 and +/- 114 gestational days, respectively. We
used placental histological sections of samples fixed in buffered saline
formaldehyde. Immunohistochemical techniques were performed to detect the apoptotic
receptors, whereas the DNA fragmentation was detected by TUNEL reaction and
apoptotic cellular ultrastructure was detected by TEM conventional techniques.
Apoptosis related receptors were immunolocalized in the early pig gestation and
correlated with apoptosis, suggesting a role in the cellular remodelling of the
placenta. At gestation day 55, apoptosis might be correlated to FAS route, but not
by DR4-mediating pathway. At the end of gestation, increased apoptosis and both
receptors markers were detected showing cellular death due to the extrinsic route
through FAS and DR4 receptors. In conclusion, the immunolocalization of FAS and TNF
R-1 receptors along the pig placental development correlates with TUNEL reaction
and with apoptotic ultrastructure observed by TEM and seems to occur through
different pathways along gestation.
La apoptosis es un proceso fisiológico, dinámico y permanente a través del cual un
organismo elimina células indeseables sin provocar una respuesta inflamatoria. El
objetivo del presente trabajo fue estudiar la expresión de los receptores de la vía
extrínseca de apoptosis, FAS, DR4 y otros miembros de la superfamilia TNF-R1, la
fragmentación del ADN y la apoptosis celular a través de TEM, en muestras
placentarias del inicio, la mitad y el final de la gestación, hacia el día +/- 30,
+/- 55 y +/- 114 de preñez, respectivamente. Se realizaron cortes histológicos de
las muestras placentarias fijadas en formol tamponado. Para la detección de los
receptores de apoptosis se realizaron técnicas inmunohistoquímicas, para el estudio
de la fragmentación del ADN se utilizó el ensayo TUNEL y para el análisis de la
ultraestructura celular apoptótica la técnica convencional de TEM. La
inmunolocalización de los receptores de muerte celular al inicio de la preñez
porcina sugiere el rol de la apoptosis en la remodelación celular placentaria.
Hacia el día 55 de preñez, la apoptosis detectada ocurriría únicamente a través de
la vía del receptor FAS, no del receptor DR4. Al final de la gestación, se detectó
un incremento de la apoptosis y la expresión de ambos receptores, indicando que la
muerte celular a través de la vía de señalización extrínseca estaría inducida por
los receptores FAS y DR4. En conclusión, la inmunolocalización de los receptores
FAS y otros miembros del TNF-R1, los resultados de TUNEL y la ultraestructura
celular apoptótica observada en la placentación porcina, indican que la apoptosis
detectada ocurre por diferentes vías de inducción a lo largo de la gestación.
AD - Merkis, C
National University of Río Cuarto. Faculty of Agronomy and Veterinary. Electronic
Microscopy Area. AR
Cristofolini, A
Consejo Nacional de Investigaciones Científicas y Técnicas. Río Cuarto. AR
Sanchis, E
Consejo Nacional de Investigaciones Científicas y Técnicas. Río Cuarto. AR
Koncurat, M
National University of Río Cuarto. Faculty of Agronomy and Veterinary. Electronic
Microscopy Area. AR
AU - Merkis, C.
AU - Cristofolini, A.
AU - Sanchis, E.
AU - Koncurat, M.
C1 - 20110318
DA - 2010/09
DB - LILACS
DO - 10.1590/S0717-95022010000300026
IS - 3
KW - Apoptosis receptors
DR4 and TNF-R1 family
DR4 y superfamilia TNF-R1
FAS/CD95
Pig placenta
Placenta porcina
Receptors de apoptosis
LA - en
PY - 2010
SN - 0717-9367
SP - 829-834
ST - Expression of death cellular receptors FAS/CD95 and DR4 during porcine
placentation
TI - Expression of death cellular receptors FAS/CD95 and DR4 during porcine
placentation
TT - Expresión de los receptores de muerte celular FAS/CD95 y DR4 durante la
placentación porcina
95022010000300026
VL - 28
ID - 4948
ER -
TY - JOUR
AB - Background: Nanosilver particles of which antibacterial and antifungal
properties have been shown in various in vitro and in vivo studies are used in many
medical and dental fields for the prevention of infection. In this study, it is
intended to evaluate the biocompatibility of nanosilver-coated brackets. Methods:
Nanosilver coating process was applied to the standard orthodontic brackets by a
physical vapor deposition system. Brackets were coated with nanosilver particles of
1 μ thickness. A total of 12 Wistar Albino rats were included in the study (six)
and control (six) groups. For the study and control groups, four nanosilver-coated
and four standard brackets were aseptically implanted subcutaneously in the dorsal
region of each rat. The brackets were removed with the surrounding tissues on days
7, 14, 30, and 60. The specimens were evaluated for inflammatory response. Results:
No significant difference was found in terms of tissue reaction between the study
and control groups. On day 7, randomly distributed brown-black granules were seen
in the granulation tissue adjacent to the bracket in the study group. These foreign
particles continued along the bracket cavity in a few samples, but the inflammatory
response was insignificant between the groups. Mast cell count was found to be
significantly smaller only on day 7 in the study group than in the control group.
Conclusions: Nanosilver-coated orthodontic brackets were found to be similar with
the standard type concerning inflammation. Further researches are needed with
regard to the assessment of the brown-black granules, especially on the deposition
of the vessel walls. © 2016, The Author(s).
AU - Metin-Gürsoy, G.
AU - Taner, L.
AU - Barış, E.
C7 - 39
DB - Scopus
DO - 10.1186/s40510-016-0152-y
IS - 1
Biomaterial science
Bracket
In vivo
Nanosilver
Animals
Cell Proliferation
Dental Alloys
Dental Implants
Epithelium
Female
Inflammation
Lymphocytes
Mandible
Mast Cells
Materials Testing
Metal Nanoparticles
Models, Animal
Orthodontic Appliance Design
Orthodontic Brackets
Plasma Cells
Rats
Rats, Wistar
Silver Compounds
Surface Properties
Time Factors
biomaterial
fibrin
dental alloy
metal nanoparticle
silver derivative
tooth implant
animal experiment
animal tissue
Article
behavior change
biocompatibility
blood vessel wall
cell count
cell infiltration
connective tissue
controlled study
female
fibroblast
foreign body
granulation tissue
histopathology
in vivo study
inflammation
mast cell
microscopy
mortality
neutrophil
nonhuman
orthodontic bracket
priority journal
rat
scoring system
weight change
animal
animal model
cell proliferation
chemistry
devices
drug effects
epithelium
lymphocyte
mandible
materials testing
orthodontic procedure
pathology
plasma cell
surface property
time factor
Wistar rat
M3 - Article
PY - 2016
ST - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
T2 - Progress in Orthodontics
TI - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
85002410873&doi=10.1186%2fs40510-016-0152-
y&partnerID=40&md5=c7f0b031068e834de306fbe3b808d2bd
VL - 17
ID - 5548
ER -
TY - JOUR
AB - Background: Nanosilver particles of which antibacterial and antifungal
properties have been shown in various in vitro and in vivo studies are used in many
medical and dental fields for the prevention of infection. In this study, it is
intended to evaluate the biocompatibility of nanosilver-coated brackets. Methods:
Nanosilver coating process was applied to the standard orthodontic brackets by a
physical vapor deposition system. Brackets were coated with nanosilver particles of
1 mu thickness. A total of 12 Wistar Albino rats were included in the study (six)
and control (six) groups. For the study and control groups, four nanosilver-coated
and four standard brackets were aseptically implanted subcutaneously in the dorsal
region of each rat. The brackets were removed with the surrounding tissues on days
7, 14, 30, and 60. The specimens were evaluated for inflammatory response. Results:
No significant difference was found in terms of tissue reaction between the study
and control groups. On day 7, randomly distributed brown-black granules were seen
in the granulation tissue adjacent to the bracket in the study group. These foreign
particles continued along the bracket cavity in a few samples, but the inflammatory
response was insignificant between the groups. Mast cell count was found to be
significantly smaller only on day 7 in the study group than in the control group.
Conclusions: Nanosilver-coated orthodontic brackets were found to be similar with
the standard type concerning inflammation. Further researches are needed with
regard to the assessment of the brown-black granules, especially on the deposition
of the vessel walls.
AN - WOS:000395249200001
AU - Metin-Gursoy, G.
AU - Taner, L.
AU - Baris, E.
C7 - 39
DA - DEC 5
DO - 10.1186/s40510-016-0152-y
PY - 2016
SN - 2196-1042
ST - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
T2 - PROGRESS IN ORTHODONTICS
TI - Biocompatibility of nanosilver-coated orthodontic brackets: an in vivo study
VL - 17
ID - 6261
ER -
TY - JOUR
AB - Ginsenoside compound K (CK) has been shown to exhibit anti-inflammatory
properties. In this study, to encourage biomedical applications of biosynthesized
gold nanoparticles (AuNPs) with anti-inflammatory effects, AuNPs loaded with
ginsenoside compound K were prepared using a self-assembly technique with chitosan
as the carrier. Optimal conditions for chitosan-ginsenoside CK-gold nanoparticles
(CS-CK-AuNPs) formation were monitored using UV-Vis absorption spectroscopy. The
physicochemical characterization of CS-CK-AuNPs was performed using FE-TEM, FE-SEM,
XRD, DLS, FTIR and NMR techniques. In the stability test, CS-CK-AuNPs did not show
any significant changes up to 4 weeks. Fluorescence imaging demonstrated that CS-
CK-AuNPs promoted cellular uptake in vitro, but did not exhibit significant
cytotoxicity at concentrations below 40 mu g/mL. Additionally, the CS-CK-AuNPs
inhibited NO production, and reduced the expression and secretion of inflammatory
cytokines (IL-1 beta, IL-6, and TNF-alpha) via inhibition of the nuclear factor-
kappaB (NF-kappa B) pathway in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
Thus, CS-CK-AuNPs are novel candidates for developing antiinflammatory agent. This
study also confirms the superiority of chitosan AuNPs as oral delivery vehicles for
inflammation-related diseases.
AN - WOS:000810262800006
AU - Mi, X. J.
AU - Choi, H. S.
AU - Park, H. R.
AU - Kim, Y. J.
C6 - JUN 2022
DA - JUL 31
DO - 10.1016/j.ijbiomac.2022.05.177
PY - 2022
SN - 0141-8130
1879-0003
SP - 247-258
ST - Structural characterization and anti-inflammatory properties of green
synthesized chitosan/compound K-gold nanoparticles
TI - Structural characterization and anti-inflammatory properties of green
synthesized chitosan/compound K-gold nanoparticles
VL - 213
ID - 6211
ER -
TY - JOUR
AB - Background: The toxicity of inhaled silver nanoparticles on contractile and
pro-inflammatory airway smooth muscle cells (ASMCs) that control airway calibre is
unknown. We explored the oxidative activities and sulfidation processes of the
toxic-inflammatory response. Method: Silver nanospheres (AgNSs) of 20 nm and 50 nm
diameter and silver nanowires (AgNWs), short S-AgNWs, 1.5 mu m and long L-AgNWs, 10
mu m, both 72 nm in diameter were manufactured. We measured their effects on cell
proliferation, mitochondrial reactive oxygen species (ROS) release and membrane
potential, and also performed electron microscopic studies. Main results and
findings: The greatest effects were observed for the smallest particles with the
highest specific surface area and greatest solubility that were avidly
internalised. ASMCs exposed to 20 nm AgNSs (25 mu g mL(-1)) for 72 hours exhibited
a significant decrease in DNA incorporation (-72.4%; p < 0.05), whereas neither the
50 nm AgNSs nor the s-AgNWs altered DNA synthesis or viability. There was a small
reduction in ASMC proliferation for the smaller AgNS, although Ag+ at 25 mu L mL(-
1) reduced DNA synthesis by 93.3% (p < 0.001). Mitochondrial potential was reduced
by both Ag+ (25 mu g mL(-1)) by 47.1% and 20 nm Ag NSs (25 mu g mL(-1)) by 40.1%
(*both at p < 0.05), but was not affected by 50 nm AgNSs and the AgNWs. None of the
samples showed a change in ROS toxicity. However, malondialdehyde release,
associated with greater total ROS, was observed for all AgNPs, to an extent
following the geometric size (20 nm AgNS: 213%, p < 0.01; 50 nm AgNS: 179.5%, p <
0.01 and L-AgNWs by 156.2%, p < 0.05). The antioxidant, N-acetylcysteine, prevented
the reduction in mitochondrial potential caused by 20 nm AgNSs. The smaller
nanostructures were internalised and dissolved within the ASMCs with the formation
of non-reactive silver sulphide (Ag2S) on their surface, but with very little
uptake of L-AgNWs. When ASMCs were incubated with H2S-producing enzyme inhibitors,
the spatial extent of Ag2S formation was much greater. Conclusion: The
intracellular toxicity of AgNPs in ASMCs is determined by the solubility of Ag+
released and the sulfidation process, effects related to particle size and
geometry. Passivation through sulfidation driven by biogenic H2S can outcompete
dissolution, thus reducing the toxicity of the smaller intracellular Ag
nanostructures.
AN - WOS:000599127600015
AU - Michaeloudes, C.
AU - Seiffert, J.
AU - Chen, S.
AU - Bey, L.
AU - Ryan, M.
AU - Cui, X. X.
AU - Zhang, J.
AU - Shaffer, M.
AU - Tetley, T.
AU - Porter, A. E.
AU - Chung, K. F.
DA - DEC 1
DO - 10.1039/d0na00745e
IS - 12
PY - 2020
SN - 2516-0230
SP - 5635-5647
ST - Effect of silver nanospheres and nanowires on human airway smooth muscle
cells: role of sulfidation
T2 - NANOSCALE ADVANCES
TI - Effect of silver nanospheres and nanowires on human airway smooth muscle
cells: role of sulfidation
VL - 2
ID - 6302
ER -
TY - JOUR
AB - Background: The toxicity of inhaled silver nanoparticles on contractile and
pro-inflammatory airway smooth muscle cells (ASMCs) that control airway calibre is
unknown. We explored the oxidative activities and sulfidation processes of the
toxic-inflammatory response. Method: Silver nanospheres (AgNSs) of 20 nm and 50 nm
diameter and silver nanowires (AgNWs), short S-AgNWs, 1.5 μm and long L-AgNWs, 10
μm, both 72 nm in diameter were manufactured. We measured their effects on cell
proliferation, mitochondrial reactive oxygen species (ROS) release and membrane
potential, and also performed electron microscopic studies. Main results and
findings: The greatest effects were observed for the smallest particles with the
highest specific surface area and greatest solubility that were avidly
internalised. ASMCs exposed to 20 nm AgNSs (25 μg mL-1) for 72 hours exhibited a
significant decrease in DNA incorporation (-72.4%; p < 0.05), whereas neither the
50 nm AgNSs nor the s-AgNWs altered DNA synthesis or viability. There was a small
reduction in ASMC proliferation for the smaller AgNS, although Ag+ at 25 μL mL-1
reduced DNA synthesis by 93.3% (p < 0.001). Mitochondrial potential was reduced by
both Ag+ (25 μg mL-1) by 47.1% and 20 nm Ag NSs (25 μg mL-1) by 40.1% (∗both at p <
0.05), but was not affected by 50 nm AgNSs and the AgNWs. None of the samples
showed a change in ROS toxicity. However, malondialdehyde release, associated with
greater total ROS, was observed for all AgNPs, to an extent following the geometric
size (20 nm AgNS: 213%, p < 0.01; 50 nm AgNS: 179.5%, p < 0.01 and L-AgNWs by
156.2%, p < 0.05). The antioxidant, N-acetylcysteine, prevented the reduction in
mitochondrial potential caused by 20 nm AgNSs. The smaller nanostructures were
internalised and dissolved within the ASMCs with the formation of non-reactive
silver sulphide (Ag2S) on their surface, but with very little uptake of L-AgNWs.
When ASMCs were incubated with H2S-producing enzyme inhibitors, the spatial extent
of Ag2S formation was much greater. Conclusion: The intracellular toxicity of AgNPs
in ASMCs is determined by the solubility of Ag+ released and the sulfidation
process, effects related to particle size and geometry. Passivation through
sulfidation driven by biogenic H2S can outcompete dissolution, thus reducing the
toxicity of the smaller intracellular Ag nanostructures. This journal is © The
AU - Michaeloudes, C.
AU - Seiffert, J.
AU - Chen, S.
AU - Bey, L.
AU - Ryan, M.
AU - Cui, X.
AU - Zhang, J.
AU - Shaffer, M.
AU - Tetley, T.
AU - Porter, A. E.
AU - Chung, K. F.
DB - Scopus
DO - 10.1039/d0na00745e
IS - 12
KW - Cell proliferation
DNA
Hydrogen sulfide
Mitochondria
Muscle
Nanospheres
Particle size
Silver nanowires
Solubility
Sulfide minerals
Sulfur compounds
Toxicity
Airway smooth muscle cells (ASMCs)
Dna incorporation
Effect of silvers
Enzyme inhibitors
Human airway smooth muscle cells
Membrane potentials
Oxidative activity
Silver compounds
M3 - Article
PY - 2020
SP - 5635-5647
ST - Effect of silver nanospheres and nanowires on human airway smooth muscle
cells: Role of sulfidation
T2 - Nanoscale Advances
TI - Effect of silver nanospheres and nanowires on human airway smooth muscle
cells: Role of sulfidation
85097942822&doi=10.1039%2fd0na00745e&partnerID=40&md5=0fd9b304b43fdca22d4a02be3f1d5
9a4
VL - 2
ID - 5353
ER -
TY - JOUR
AB - The antimicrobial properties of silver nanoparticles (AgNPs) have made these
particles one of the most frequently utilized nanomaterials in consumer products;
therefore, a comprehensive understanding of their toxicity is necessary. In
particular, information about the cellular uptake and size dependence of AgNPs is
insufficient.In this study, we evaluated the size-dependent effects of AgNPs by
treating the human LoVo cell line, an intestinal epithelium model, with spherical
AgNPs of well-defined sizes (10, 20, 40, 60 and 100. nm). The cellular uptake was
visualized by confocal laser scanning microscopy, and various cytotoxicity
parameters were analyzed in a size- and dose-dependent manner. In addition, the
cellular proteomic response to 20 and 100. nm AgNPs was investigated to increase
the understanding of potential mechanisms of action. Our data indicated that
cellular uptake and toxicity were regulated by size; smaller particles easily
penetrated the cells, and 100. nm particles did not. It was hypothesized that this
size-dependent effect resulted from the stimulation of a signaling cascade that
generated ROS and inflammatory markers, leading to mitochondrial dysfunction and
subsequently inducing apoptosis. By contrast, the cell proliferation, was
independent of AgNPs particle size, indicating a differentially regulated, ROS-
independent pathway. © 2014 Elsevier Ltd.
AU - Miethling-Graff, R.
AU - Rumpker, R.
AU - Richter, M.
AU - Verano-Braga, T.
AU - Kjeldsen, F.
AU - Brewer, J.
AU - Hoyland, J.
AU - Rubahn, H. G.
AU - Erdmann, H.
DB - Scopus
DO - 10.1016/j.tiv.2014.06.005
IS - 7
KW - Cellular uptake
Cytotoxicity
LoVo cell line
Apoptosis
Carcinoma
Cell Line, Tumor
Cell Survival
Colonic Neoplasms
Humans
Interleukin-8
Metal Nanoparticles
Oxidative Stress
Particle Size
Proteomics
Silver
silver nanoparticle
IL8 protein, human
interleukin 8
metal nanoparticle
silver
apoptosis
article
carcinoma cell line
cell proliferation
cell transport
cell viability
colon carcinoma
confocal laser microscopy
controlled study
cytokine release
cytotoxicity
human
human cell
inflammation
mitochondrial dynamics
oxidative stress
particle size
proteomics
signal transduction
carcinoma
cell survival
colon tumor
drug effects
metabolism
tumor cell line
M3 - Article
PY - 2014
SP - 1280-1289
ST - Exposure to silver nanoparticles induces size- and dose-dependent oxidative
stress and cytotoxicity in human colon carcinoma cells
TI - Exposure to silver nanoparticles induces size- and dose-dependent oxidative
stress and cytotoxicity in human colon carcinoma cells
84896990181&doi=10.1016%2fj.tiv.2014.06.005&partnerID=40&md5=2af51b5d9bf2934e2328ec
4cf9e98e47
VL - 28
ID - 5657
ER -
TY - JOUR
AB - For decades, nanomedicines have been reported as a potential means to
overcome the limitations of conventional drug delivery systems by reducing side
effects, toxicity and the non-ideal pharmacokinetic behaviour typically exhibited
by small molecule drugs. However, upon administration many nanoparticles prompt
induction of host inflammatory responses due to recognition and uptake by
macrophages, eliminating up to 95% of the administered dose. While significant
advances in nanoparticle engineering and consequent therapeutic efficacy have been
made, it is becoming clear that nanoparticle recognition by the mononuclear
phagocyte system (MPS) poses an impassable junction in the current framework of
nanoparticle development. Hence, this has negative consequences on the clinical
translation of nanotechnology with respect to therapeutic efficacy, systemic
toxicity and economic benefit. In order to improve the translation of nanomedicines
from bench-to-bedside, there is a requirement to either modify nanomedicines in
terms of how they interact with intrinsic processes in the body, or modulate the
body to be more accommodating for nanomedicine treatments. Here we provide an
overview of the current standard for design elements of nanoparticles, as well as
factors to consider when producing nanomedicines that have minimal MPS-nanoparticle
interactions; we explore this landscape across the cellular to tissue and organ
levels. Further, rather than designing materials to suit the body, a growing
research niche involves modulating biological responses to administered
nanomaterials. We here discuss how developing strategic methods of MPS 'pre-
conditioning' with small molecule or biological drugs, as well as implementing
strategic dosing regimens, such as 'decoy' nanoparticles, is essential to
increasing nanoparticle therapeutic efficacy. By adopting such a perspective, we
hope to highlight the increasing trends in research dedicated to improving
nanomedicine translation, and subsequently making a positive clinical impact.
AN - WOS:000782246800001
AU - Mills, J. A.
AU - Liu, F. F.
AU - Jarrett, T. R.
AU - Fletcher, N. L.
AU - Thurecht, K. J.
C6 - APR 2022
DA - JUN 14
DO - 10.1039/d2bm00181k
IS - 12
PY - 2022
SN - 2047-4830
2047-4849
SP - 3029-3053
ST - Nanoparticle based medicines: approaches for evading and manipulating the
mononuclear phagocyte system and potential for clinical translation
TI - Nanoparticle based medicines: approaches for evading and manipulating the
mononuclear phagocyte system and potential for clinical translation
VL - 10
ID - 6798
ER -
TY - JOUR
AN - WOS:000649382400028
AU - Mimura, E. C. M.
AU - Carrilho, A. J. F.
C6 - MAY 2021
DA - MAY
DO - 10.1016/j.burns.2020.11.001
IS - 3
PY - 2021
SN - 0305-4179
1879-1409
SP - 736-737
ST - Reply to comments on "Silver serum levels in burned patients treated with
silver sulfadiazine and its toxicity on inflammatory cells"
T2 - BURNS
TI - Reply to comments on "Silver serum levels in burned patients treated with
silver sulfadiazine and its toxicity on inflammatory cells"
VL - 47
ID - 6386
ER -
TY - JOUR
DB - Scopus
DO - 10.1016/j.burns.2020.11.001
IS - 3
KW - Burns
Humans
Silver
Silver Sulfadiazine
silver
sulfadiazine silver
blood level
burn
burn patient
cream
drug absorption
drug cytotoxicity
electric burn
human
inflammatory cell
injury severity
Letter
surgeon
thickness
total body surface area
M3 - Letter
PY - 2021
SP - 736-737
ST - Reply to comments on “Silver serum levels in burned patients treated with
silver sulfadiazine and its toxicity on inflammatory cells”
T2 - Burns
TI - Reply to comments on “Silver serum levels in burned patients treated with
silver sulfadiazine and its toxicity on inflammatory cells”
85097912729&doi=10.1016%2fj.burns.2020.11.001&partnerID=40&md5=083edc6311956350d2c6
72ab36fd1082
VL - 47
ID - 5206
ER -
TY - JOUR
AB - Background: Silver sulfadiazine (SSD) has been widely used in burned patients
for the prevention of local infections. To be biologically active and exert
antimicrobial properties, silver needs to be present in the form of silver ions
(Ag1+) that bind to negatively charged proteins, namely, the RNA and DNA in
microorganisms. However, previous published studies conducted with SSD in the 1990s
reported a high level of silver absorption through damaged skin and noted the
potential cytotoxicity of Ag1+ to human cells. SSD toxicity, however, had been
described in cell cultures using arbitrary silver concentrations. In the present
study, we determined the serum silver levels in burned patients treated with SSD
and, taking into account the molar Ag1+ concentrations found in these patients, we
evaluated the Ag1+ toxicity effects on inflammatory cells (ROS and cytokine
production) in vitro. Methods: Twenty patients with an average burned body surface
area of 27.68% were included in this study. Results: Patients’ Ag1+ serum levels
reached up to 558 times those of the unexposed controls. Ag1+ was then added to
inflammatory cells in vitro at levels up to 2000 times the level of the control,
and there was no effect on the viability of the cells nor on the rate of apoptosis.
We observed a decrease in reactive oxygen species production by mononuclear (MN)
and polymorphonuclear (PMN) cells, as well as a substantial decrease in cytokines
IL-1β, IL-6, IL-8, IL-10, and TNF-α production by leukocytes (MN and PNM).
Conclusion: These findings suggest that Ag1+ may contribute to negative outcomes
after burns, decreasing the primary defense mechanism (respiratory burst) and
altering cytokine production. © 2019 Elsevier Ltd and ISBI
AU - Favoreto, J. P. M.
AU - Favero, M. E.
AU - Bonifacio, K. L.
AU - Peixe, T. S.
AU - Morita, A. A.
AU - Barbosa, D. S.
AU - Yabe, M. J. S.
DB - Scopus
DO - 10.1016/j.burns.2019.11.012
IS - 5
KW - Burns
Interleukins
Respiratory burst
Silver sulfadiazine
Silver toxicity
Adult
Apoptosis
Body Surface Area
Cell Survival
Female
Humans
In Vitro Techniques
Interleukin-10
Interleukin-1beta
Interleukin-6
Interleukin-8
Male
Neutrophils
Silver
Silver Nitrate
Silver Sulfadiazine
interleukin 10
interleukin 1beta
interleukin 6
interleukin 8
silver
sulfadiazine silver
IL10 protein, human
IL1B protein, human
IL6 protein, human
silver nitrate
TNF protein, human
topical antiinfective agent
adult
Article
burn patient
clinical article
controlled study
cytokine production
drug absorption
drug blood level
drug cytotoxicity
female
human
human cell
in vitro study
inflammatory cell
male
mononuclear cell
polymorphonuclear cell
prospective study
apoptosis
blood
body surface
burn
cell survival
drug effect
metabolism
neutrophil
M3 - Article
PY - 2020
SP - 1120-1127
ST - Silver serum levels in burned patients treated with silver sulfadiazine and
its toxicity on inflammatory cells
T2 - Burns
TI - Silver serum levels in burned patients treated with silver sulfadiazine and
its toxicity on inflammatory cells
85076468978&doi=10.1016%2fj.burns.2019.11.012&partnerID=40&md5=93bf739742daabb6cb22
6911cfb7ddde
VL - 46
ID - 5262
ER -
TY - JOUR
AB - Emerging nanoscience allows us to take advantage of the improved evolutionary
components and apply today's advanced characterization and fabrication techniques
to solve environmental and biological problems. Despite the promise that
nanotechnology will improve our lives, the potential risks of technology remain
largely uncertain. The lack of information on bio-impacts and the absence of
consistent standards are the limitations of using metal-based nanoparticles (mNPs)
for existing applications. To analyze the role played by the mNPs physicochemical
characteristics and tactics to protect live beings, the field of nanotoxicology
nowadays is focused on collecting and analyzing data from in vitro and in vivo
investigations. The degree of reactive oxygen species (ROS) and oxidative stress
caused by material nanoparticles (NPs) depends on many factors, such as size,
shape, chemical composition, etc. These characteristics enable NPs to enter cells
and interact with biological macromolecules and cell organelles, resulting in
oxidative damage, an inflammatory response, the development of mitochondrial
dysfunction, damage to genetic material, or cytotoxic effects. This report explored
the mechanisms and cellular signaling cascades of mNPs-induced oxidative stress and
the relevant health consequences.
AN - WOS:000957346700001
AU - Min, Y. H.
AU - Suminda, G. G. D.
AU - Heo, Y.
AU - Kim, M.
AU - Ghosh, M.
AU - Son, Y. O.
C7 - 703
DA - MAR
DO - 10.3390/antiox12030703
IS - 3
PY - 2023
SN - 2076-3921
ST - Metal-Based Nanoparticles and Their Relevant Consequences on Cytotoxicity
Cascade and Induced Oxidative Stress
T2 - ANTIOXIDANTS
TI - Metal-Based Nanoparticles and Their Relevant Consequences on Cytotoxicity
Cascade and Induced Oxidative Stress
VL - 12
ID - 6486
ER -
TY - CHAP
AB - Nanotechnology has emerged as a key player in various disciplines of science
and technology. Some of the most exciting applications are in the field of
biomedicine—for theranostics (for combined diagnostic and therapeutic purposes) and
for exploration of biological systems. A detailed understanding of the molecular
interactions between nanoparticles and biological nanomachinery—macromolecules,
membranes, and intracellular organelles—is crucial for obtaining adequate
information on mechanisms of action of nanomaterials and a perspective on the long-
term effects of these materials and their possible toxicological outcomes. This
chapter focuses on the toxicological aspects of nanoparticles and carbon nanotubes
on animals, humans, plants, and aquatic life. Generally, the harmful effects of
nanoparticles arise from the combination of various factors, two of which are
particularly important: (a) the high surface area and (b) the intrinsic toxicity of
the surface. In contrast with conventional particles of larger mean diameter,
nanoparticles under 100nm can potentially be more toxic to the lung (portal of
entry), can redistribute from their site of deposition, can escape from the normal
phagocytic defenses, and can modify the structure of proteins. Therefore,
nanoparticles can activate inflammatory and immunologic responses and may affect
the normal tissue function. CNT, in the context of toxicology, can be classified as
“nanoparticles” due to their nanoscale dimensions; therefore, unexpected
toxicological effects upon contact with biological systems may be induced. © 2019
AU - Mishra, R.
AU - Militky, J.
AU - Arumugam, V.
DB - Scopus
DO - 10.1016/B978-0-08-102609-0.00008-0
KW - Antibacterial nanoparticles
Carbon nanotubes
Inhalation
Life-cycle assessment
MWCNT
Nanoparticles
Toxicology
PY - 2018
SP - 355-385
ST - Nanorisks and nanohazards
T2 - Nanotechnology in Textiles: Theory and Application
TI - Nanorisks and nanohazards
85142090051&doi=10.1016%2fB978-0-08-102609-0.00008-
0&partnerID=40&md5=4a45e0051aecb7a8cf6f5e6d5fcf3c15
ID - 5464
ER -
TY - JOUR
AB - An in situ synthesis method for preparing silver nanoclusters (AgNCs)
embedded in chitosan-polyethylene glycol (CS-PEG) membranes is disclosed. The aim
is to develop implantable multifunctional devices for biofilm inhibition and drug
release to reduce percutaneous device related complications (PDRCs). A multiple
array of characterization techniques confirmed the formation of fluorescent AgNCs
with sizes of ∼3 nm uniformly distributed in CS-PEG matrix and their active role in
determining the fraction and interconnectivity of the microporous membranes. The
presence and increasing contents of AgNCs enhanced the mechanical stability of
membranes and decreased their susceptibility to degradation in the presence of
lysozyme and H2O2. Moreover, the presence and increasing concentrations of AgNCs
hindered biofilm formation against Escherichia coli (Gram negative) and
Staphylococcus aureus (Gram positive) and enabled a sustainable release of an anti-
inflammatory drug naproxen in vitro until 24 h. The overall results gathered and
reported in this work make the AgNCs impregnated CS-PEG membranes highly promising
multifunctional devices combining efficient antibacterial activity and
biocompatibility with active local drug delivery. © 2016 American Chemical Society.
AU - Mishra, S. K.
AU - Raveendran, S.
AU - Ferreira, J. M. F.
AU - Kannan, S.
DB - Scopus
DO - 10.1021/acs.langmuir.6b02844
IS - 40
KW - Administration, Cutaneous
Animals
Biofilms
Cattle
Cell Line, Tumor
Chickens
Chitosan
Drug Carriers
Drug Liberation
Elastic Modulus
Escherichia coli
Humans
Hydrogen Peroxide
Metal Nanoparticles
Muramidase
Naproxen
Particle Size
Silver
Surface Properties
Bacteria
Biocompatibility
Chitin
Mechanical stability
Microporosity
Nanoclusters
antiinfective agent
chitosan
drug carrier
hen egg lysozyme
hydrogen peroxide
lysozyme
macrogol
metal nanoparticle
naproxen
silver
Biofilm inhibitions
Micro porous membranes
Multifunctional devices
Percutaneous devices
animal
artificial membrane
biofilm
bovine
chemistry
chicken
cutaneous drug administration
drug effect
drug release
human
particle size
surface property
synthesis
tumor cell line
Young modulus
Membranes
M3 - Article
PY - 2016
SP - 10305-10316
ST - In situ impregnation of silver nanoclusters in microporous chitosan-PEG
membranes as an antibacterial and drug delivery percutaneous device
T2 - Langmuir
TI - In situ impregnation of silver nanoclusters in microporous chitosan-PEG
membranes as an antibacterial and drug delivery percutaneous device
84991503821&doi=10.1021%2facs.langmuir.6b02844&partnerID=40&md5=f0fd3a42a6026940a2b
0ff47e4685231
VL - 32
ID - 5469
ER -
TY - JOUR
AB - The clinical success of coated implants in executing biological functions
inclusive of sustainable drug release and long term antibacterial activity without
antibiotics is critical. To this aim, a nanohybrid of silver nanoparticles (AgNPs)
cored in polyvinyl alcohol nanocapsules (Ag-PVA NCs) embedded in chitosan (CS)
matrix loaded with anti-inflammatory drug naproxen was prepared. The synthesized
nanohybrids that were subjected to coatings on (3-aminopropyl)triethoxysilane
(APTES) treated titanium (Ti) metal exhibited dual role of excellent inhibition on
biofilm formation and sustained drug release. These dual characteristics are
achieved mainly based on intrinsic antibacterial property of AgNPs and differential
entrapment of drug in PVA polymeric shell of AgNPs and CS matrix. The coatings also
demonstrated enhanced mechanical properties with increasing inorganic filler and
stress shielding on Ti metal. The biocompatibility tests involving adhesion,
proliferation and differentiation of osteoblast cells demonstrated the efficacy of
Ag-PVA NCs embedded in CS matrix as a suitable coating material for orthopedic
applications. © 2016 Elsevier Inc.
AU - Mishra, S. K.
AU - Teotia, A. K.
AU - Kumar, A.
AU - Kannan, S.
DB - Scopus
DO - 10.1016/j.nano.2016.08.010
IS - 1
KW - Chitosan
Microwave synthesis
Nanohybrid
Nanoindentation
Naproxen
Biofilms
Cell Line
Cell Proliferation
Drug Liberation
Humans
Metal Nanoparticles
Nanocomposites
Osteoblasts
Silver
Titanium
Biocompatibility
Biomechanics
Cell proliferation
Chitin
Coatings
Drug products
Inorganic coatings
Metal nanoparticles
Nanoparticles
Stresses
chitosan
nanocapsule
nanocomposite
nanoshell
naproxen
polymer
polyvinyl alcohol
silver nanoparticle
titanium
antiinfective agent
metal nanoparticle
silver
Nano hybrids
Naproxens
Article
biofilm
cell adhesion
cell proliferation
controlled study
drug synthesis
human
human cell
microwave irradiation
nanofabrication
nonhuman
osteoblast
Raman spectrometry
cell line
chemistry
cytology
drug effects
drug release
M3 - Article
PY - 2017
SP - 23-35
ST - Mechanically tuned nanocomposite coating on titanium metal with integrated
properties of biofilm inhibition, cell proliferation, and sustained drug delivery
TI - Mechanically tuned nanocomposite coating on titanium metal with integrated
properties of biofilm inhibition, cell proliferation, and sustained drug delivery
84997017376&doi=10.1016%2fj.nano.2016.08.010&partnerID=40&md5=cef5d3ad4e186802a66d5
d5116a257b5
VL - 13
ID - 5555
ER -
TY - JOUR
AB - Copper oxide nanoparticles with different shapes were used to examine the
effect of shape on the various physicochemical properties (reactivity, aggregation,
suspension stability) and to examine the behaviour by which CuO nanoparticles
exhibit their biological response towards alveolar type-I cells. The different
shapes examined in this study include spherical-, rod- and spindle-shaped platelet
particles. In vitro dissolution studies (7 days) in 1 mM NaNO3 matrix showed a
marked difference in dissolved Cu release between the nanoparticles. However, in
serum-free cell-culture media (exposure media to cells), the particles' dissolution
was found to be significantly enhanced with close to complete dissolution reported
for all particle types. Biological studies showed both shape and size of the CuO
nanoparticles tested to have a significant effect on TT-1 cell viability and
release of pro-inflammatory cytokines IL-6 and IL-8. This study shows a complex
interplay between particulate and dissolved species triggering the biological
response. Upon immediate exposure of CuO nanoparticles of different shapes, the
particulate form contributes towards the toxicity. However, for any biological
response observed over and beyond a period of 24 h, the dissolved fraction becomes
significant.
AN - WOS:000328126700007
AU - Misra, S. K.
AU - Nuseibeh, S.
AU - Dybowska, A.
AU - Berhanu, D.
AU - Tetley, T. D.
AU - Valsami-Jones, E.
DA - JUN
DO - 10.3109/17435390.2013.796017
IS - 4
PY - 2014
SN - 1743-5390
1743-5404
SP - 422-432
ST - Comparative study using spheres, rods and spindle-shaped nanoplatelets on
dispersion stability, dissolution and toxicity of CuO nanomaterials
T2 - NANOTOXICOLOGY
TI - Comparative study using spheres, rods and spindle-shaped nanoplatelets on
dispersion stability, dissolution and toxicity of CuO nanomaterials
VL - 8
ID - 6538
ER -
TY - JOUR
AB - Nanoparticles(NPs) are of particle sizes lesser than 100nm and are insoluble
the field which deal with the handling of these particles is coined as
"Nanotechnology" as their particle size is smaller, they can penetrate easily
therefore they are applied in various medical fields, drug delivery and in
dentistry as they have antimicrobial property, reduces friction, anti-inflammatory
and antioxidant property. Many studies have been done to evaluate its application
and its cytotoxicity by varying its concentration and various studies have been
done to evaluate its physical property. Therefore, it is of interest to describe
concepts of nanoparticles, mode of action, tissue reaction and its application in
orthodontics.
AN - WOS:000975608100004
AU - Missier, M. S.
AU - Ramakrishnan, M.
AU - Paulpandian, S. D. S.
AU - Pringle, J.
DA - JAN
DO - 10.6026/97320630019014
IS - 1
PY - 2023
SN - 0973-8894
0973-2063
SP - 14-18
ST - Application of nanoparticles in Dentistry
T2 - BIOINFORMATION
TI - Application of nanoparticles in Dentistry
VL - 19
ID - 6510
ER -
TY - CHAP
AB - The health effects of silver nanoparticles (AgNPs) have not been well
investigated, despite AgNPs now being widely used in consumer products. We
introduce living environment, analysis, metabolic behavior, toxicity, and human
health effect of AgNPs in comparison to silver nitrate (AgNO3). The American
Conference of Governmental Industrial Hygienists (ACGIH) has established separate
threshold limit values (TLV) for metallic silver (0.1 mg/m3) and soluble compounds
of silver (0.01 mg/m3). Argyria and argyrosis are chronic disorders of skin
microvessels and eyes in humans, and these disorders reportedly develop following
extended oral and inhalational exposure to Ag. In mammals, AgNO3 and AgNPs
increased the number of the total cells, neutrophils, and pro-inflammatory cytokine
production "IL-1β," and these were distributed in the lung, kidney, and liver. The
amount of Ag in the metallothionein (MT)-bound form was related in cellular
behavior and toxicity of AgNPs and AgNO3. The cytotoxic effect of AgNPs is a simple
function of neither the number nor total surface area. Although the effect may vary
among the cell types and the culture conditions, AgNPs were transported to
lysosomes and only gradually dissolved in mammals, causing milder inflammatory
stimulation. © Springer Japan 2016. All rights are reserved.
AU - Miyayama, T.
AU - Arai, Y.
AU - Hirano, S.
DB - Scopus
DO - 10.1007/978-4-431-55732-6_7
KW - Lysosome
Macrophages
Metallothionein
Silver ion
Silver nanoparticle
PY - 2015
SP - 137-147
ST - Health effects of silver nanoparticles and silver ions
T2 - Biological Effects of Fibrous and Particulate Substances
TI - Health effects of silver nanoparticles and silver ions
84956475031&doi=10.1007%2f978-4-431-55732-
6_7&partnerID=40&md5=5b4738c2cb67b83dd127ca292445b0ff
ID - 5652
ER -
TY - JOUR
AB - Bacteriocins are low molecular weight substances produced through post
transcriptional changes. These molecules are easily degraded in mammalian gut by
proteolytic enzymes especially protease. Nisin is a peptide with 34 aa and its
structure contains a pentacyclic lanthionine and 4 beta metyllanthionine residues.
Different formulations have been designed for nisin. Since "green synthesis" is a
progressive method to prepare anti-microbial and anti-cancer compounds, this study
aimed at green synthesis of nisin metal compounds to be used lower concentration
still exerting nisin effects. For this purpose, a 1 mg/ml nisin solution was added
to a 1 mM silver nitrate solution and incubated to synthesis nano Ag-nisin, then
the optical density of new solution was detected using UV spectroscopy. To
determine biomolecules in the Ag-nisin solution, the FTIR method was employed. The
size and morphology of Ag-nisin was measured by TEM. The toxicity, inflammatory
cytokines production, and intracellular ROS quantity was evaluated using MTT, ELISA
and flow-cytometry. XRD pattern indicated the silver crystals in Ag-nisin solution.
In addition, FTRI findings showed that the carbonyl groups of amino acid are
potently able to bind to metal nanoparticles, cover, and prevent them from particle
agglomeration. Treating macrophage cells with 10, 25, 50 and 100 mu g/ml of Ag-
nisin had no significant effect on the cell viability and intracellular ROS
quantity compared to the control group. In addition, different concentrations of
Ag-nisin had no effect on the IL-10 and TNF-alpha levels but caused an increased
level of IL-12 in comparison with the control group. In the current study, for the
first time, green synthesize was used to prepare Ag-nisin particles. The
synthesized nanoparticle is able to induce inflammatory activity via increasing IL-
12 without any change in the TNF-alpha level in macrophage cells.
AN - WOS:000426226700064
AU - Moein, M.
AU - Fooladi, A. A. I.
AU - Hosseini, H. M.
DA - JAN
DO - 10.1016/j.micpath.2017.12.034
PY - 2018
SN - 0882-4010
SP - 414-419
ST - Determining the effects of green chemistry synthesized Ag-nisin nanoparticle
on macrophage cells
T2 - MICROBIAL PATHOGENESIS
TI - Determining the effects of green chemistry synthesized Ag-nisin nanoparticle
on macrophage cells
VL - 114
ID - 6503
ER -
TY - JOUR
AB - Bacteriocins are low molecular weight substances produced through post
transcriptional changes. These molecules are easily degraded in mammalian gut by
proteolytic enzymes especially protease. Nisin is a peptide with 34 aa and its
structure contains a pentacyclic lanthionine and 4 beta metyllanthionine residues.
Different formulations have been designed for nisin. Since “green synthesis” is a
progressive method to prepare anti-microbial and anti-cancer compounds, this study
aimed at green synthesis of nisin metal compounds to be used lower concentration
still exerting nisin effects. For this purpose, a 1 mg/ml nisin solution was added
to a 1 mM silver nitrate solution and incubated to synthesis nano Ag-nisin, then
the optical density of new solution was detected using UV spectroscopy. To
determine biomolecules in the Ag-nisin solution, the FTIR method was employed. The
size and morphology of Ag-nisin was measured by TEM. The toxicity, inflammatory
cytokines production, and intracellular ROS quantity was evaluated using MTT, ELISA
and flow-cytometry. XRD pattern indicated the silver crystals in Ag-nisin solution.
In addition, FTRI findings showed that the carbonyl groups of amino acid are
potently able to bind to metal nanoparticles, cover, and prevent them from particle
agglomeration. Treating macrophage cells with 10, 25, 50 and 100 μg/ml of Ag-nisin
had no significant effect on the cell viability and intracellular ROS quantity
compared to the control group. In addition, different concentrations of Ag-nisin
had no effect on the IL-10 and TNF-α levels but caused an increased level of IL-12
in comparison with the control group. In the current study, for the first time,
green synthesize was used to prepare Ag-nisin particles. The synthesized
nanoparticle is able to induce inflammatory activity via increasing IL-12 without
any change in the TNF-α level in macrophage cells. © 2017
AU - Moein, M.
AU - Imani Fooladi, A. A.
AU - Mahmoodzadeh Hosseini, H.
DB - Scopus
DO - 10.1016/j.micpath.2017.12.034
KW - Bacteriocin
Green synthesis
Nisin
Probiotic
Bacteriocins
Cell Survival
Cytokines
Interleukin-10
Interleukin-12
Macrophages
Metal Nanoparticles
Particle Size
Silver
Silver Nitrate
X-Ray Diffraction
carbonyl derivative
cytokine
interleukin 10
nisin
silver nanoparticle
silver nisin nanoparticle
silver nitrate
unclassified drug
bacteriocin
interleukin 12
metal nanoparticle
silver
animal cell
Article
cell viability
controlled study
cytokine production
flow cytometry
green chemistry
macrophage
morphology
mouse
MTT assay
nonhuman
optical density
particle size
priority journal
toxicity testing
X ray diffraction
biosynthesis
cell survival
chemistry
drug effect
metabolism
procedures
ultrastructure
M3 - Article
PY - 2018
SP - 414-419
ST - Determining the effects of green chemistry synthesized Ag-nisin nanoparticle
on macrophage cells
T2 - Microbial Pathogenesis
TI - Determining the effects of green chemistry synthesized Ag-nisin nanoparticle
on macrophage cells
85038019185&doi=10.1016%2fj.micpath.2017.12.034&partnerID=40&md5=e68cc902b3f6ef8c23
ea1ab5da68763d
VL - 114
ID - 5556
ER -
TY - JOUR
AB - This study consisted of developing a dressing loaded with silver (Ag) and
ibuprofen (IBU) that provides a dual therapy, antibacterial and antalgic, intended
for infected painful wounds. There-fore, non-woven polyethyleneterephtalate (PET)
textiles nonwovens were pre-treated by cyclodextrin crosslinked with citric acid by
a pad/dry/cure process. Then, textiles were impregnated in silver so-lution
followed by a thermal treatment and were then coated by Layer-by-Layer (L-b-L)
deposition of a polyelectrolyte multilayer (PEM) system consisting of anionic
water-soluble poly(betacyclodextrin citrate) (PCD) and cationic chitosan. Finally,
ibuprofen lysinate (IBU-L) was loaded on the PEM coating. We demonstrated the
complexation of IBU with native βCD and PCD by phase solubility diagram and1 H NMR.
PEM system allowed complete IBU-L release in 6 h in PBS pH 7.4 batch (USP IV). On
the other hand, microbiological tests demonstrated that loaded silver induced
bacterial reduction of 4 Log10 against S. aureus and E. coli and tests revealed
that ibuprofen lysinate loading did not interfere with the antibacterial properties
of the dressing. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Mogrovejo-Valdivia, A.
AU - Maton, M.
AU - Garcia-Fernandez, M. J.
AU - Tabary, N.
AU - Chai, F.
AU - Neut, C.
AU - Martel, B.
AU - Blanchemain, N.
C7 - 805
DB - Scopus
IS - 7
Antibacterial
Cyclodextrins
Drug delivery system
Layer by layer
Wound dressing
antibiotic agent
antiinfective agent
chitosan
citric acid
cyclodextrin
ibuprofen
ibuprofen lysine
polycaprolactone
polyelectrolyte
polyethylene terephthalate
silver
silver nanoparticle
silver sulfate
unclassified drug
water
Article
biological activity
cell viability
chemical reaction kinetics
colony forming unit
complex formation
controlled study
cross linking
cytotoxicity
diffusion
disk diffusion
drug absorption
drug activity
drug release
drug solubility
echography
electrospinning
encapsulation
Escherichia coli
in vitro study
isotherm
isothermal amplification
layer by layer deposition
mathematical analysis
microbiological examination
nonhuman
pH
solubility
ultraviolet microscopy
wound healing
zone of inhibition
M3 - Article
PY - 2021
ST - In vitro microbiological and drug release of silver/ibuprofen loaded wound
dressing designed for the treatment of chronically infected painful wounds
T2 - Antibiotics
TI - In vitro microbiological and drug release of silver/ibuprofen loaded wound
dressing designed for the treatment of chronically infected painful wounds
85110279038&doi=10.3390%2fantibiotics10070805&partnerID=40&md5=9014356407e6e2eb7403
f6c0b262f120
VL - 10
ID - 5232
ER -
TY - JOUR
AB - Background: The excessive exposure to silver nanoparticles (Ag-NPs) has
raised concerns about their possible risks to the human health. The brain is a
highly vulnerable organ to nano-silver harmfulness. The aim of this work was to
evaluate the impacts of Ag-NPs exposure on the cerebellar cortex of rats. Methods:
Rats were assigned to: Control, vehicle control and Ag-NP-exposed groups (at doses
of 10 mg and 30 mg/kg/day). Samples were processed for light and electron
microscopy examinations. Immunohistochemical localization of c-Jun N-terminal
kinase (JNK), nuclear factor kappa beta (NF-κB) and calbindin D28k (CB) proteins
was performed. Analyses of expression of DNA damage inducible transcript 4 (Ddit4),
flavin containing monooxygenase 2 (FMO2) and thioredoxin-interacting protein
(Txnip) genes were done. Serum levels of inflammatory cytokines were also measured.
Results: Ag-NPs enhanced apoptosis as evident by upregulation of Ddit4 gene
expressions and JNK protein immune expressions. Alterations of redox homeostasis
were verified by enhancement of Txnip and FMO2 gene expressions, favoring the
activation of inflammatory responses by increasing NF-κB protein immune expressions
and serum inflammatory mediator levels. Another cytotoxic effect was the reduction
of immune expressions of the calcium regulator CB. Conclusion: Ag-NPs exposure
provoked biochemical, cellular and molecular changes of rat cerebellar cortex in a
dose-dependent manner. © 2020 by the authors. Li-censee MDPI, Basel, Switzerland.
AU - Mohamed, E. M.
AU - Kattaia, A. A. A.
AU - Abdul-Maksoud, R. S.
AU - Abd El-Baset, S. A.
C7 - 7
DB - Scopus
DO - 10.3390/cells10010007
IS - 1
KW - Cerebellar cortex
Impacts
Rat
Animals
Apoptosis
Biomarkers
Cerebellar Cortex
Male
Metal Nanoparticles
NF-kappa B
Oxidative Stress
Rats
Rats, Wistar
Silver
calbindin 1
interleukin 6
silver nanoparticle
biological marker
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
cerebellar tissue
controlled study
electron microscopy
gene expression
histopathology
inflammation
male
nonhuman
oxidative stress
protein expression
rat
RNA extraction
animal
apoptosis
cerebellum cortex
drug effect
metabolism
Wistar rat
M3 - Article
PY - 2021
SP - 1-19
ST - Cellular, molecular and biochemical impacts of silver nanoparticles on rat
cerebellar cortex
T2 - Cells
TI - Cellular, molecular and biochemical impacts of silver nanoparticles on rat
cerebellar cortex
85099115546&doi=10.3390%2fcells10010007&partnerID=40&md5=407c7d419f2aaeade97b548bcc
c1c862
VL - 10
ID - 5312
ER -
TY - JOUR
AB - Engineered nanoparticles (ENP), which could be composed of inorganic metals,
metal oxides, metalloids, organic biodegradable and inorganic biocompatible
polymers, are being used as carriers for vaccine and drug delivery. There is also
increasing interest in their application as delivery agents for the treatment of a
variety of lung diseases. Although many studies have shown ENP can be effectively
and safely used to enhance the delivery of drugs and vaccines in the periphery,
there is concern that some ENP could promote inflammation, with unknown
consequences for lung immune homeostasis. In this study, we review research on the
effects of ENP on lung immunity, focusing on recent studies using diverse animal
models of human lung disease. We summarize how the inflammatory and immune response
to ENP is influenced by the diverse biophysical and chemical characteristics of the
particles including composition, size and mode of delivery. We further discuss
newly described unexpected beneficial properties of ENP administered into the lung,
where biocompatible polystyrene or silver nanoparticles can by themselves decrease
susceptibility to allergic airways inflammation. Increasing our understanding of
the differential effects of diverse types of nanoparticles on pulmonary immune
homeostasis, particularly previously underappreciated beneficial outcomes, supports
rational ENP translation into novel therapeutics for prevention and/or treatment of
inflammatory lung disorders. © 2014 Informa Healthcare USA, Inc. All rights
reserved: reproduction in whole or part not permitted.
AU - Mohamud, R.
AU - Xiang, S. D.
AU - Selomulya, C.
AU - Rolland, J. M.
AU - O'Hehir, R. E.
AU - Hardy, C. L.
AU - Plebanski, M.
DB - Scopus
DO - 10.3109/03602532.2013.859688
IS - 2
KW - Animal models
Delivery vehicles
Immune response
Inflammation
Lung
Particles
Toxicity
Vaccine
Adaptive Immunity
Animals
Drug Carriers
Homeostasis
Humans
Immunity, Innate
Nanoparticles
Particle Size
Pneumonia
Surface Properties
carbon nanotube
drug vehicle
gold nanoparticle
iron oxide
liposome
nanoparticle
polysaccharide
polystyrene
silicon dioxide
silver nanoparticle
titanium dioxide
zinc oxide
drug carrier
adaptive immunity
biocompatibility
dendritic cell
homeostasis
human
immune response
immunity
inflammation
innate immunity
lung function
nonhuman
physical chemistry
pneumonia
review
animal
chemistry
drug effects
immunology
lung
particle size
surface property
M3 - Review
PY - 2014
SP - 176-190
ST - The effects of engineered nanoparticles on pulmonary immune homeostasis
T2 - Drug Metabolism Reviews
TI - The effects of engineered nanoparticles on pulmonary immune homeostasis
84899439295&doi=10.3109%2f03602532.2013.859688&partnerID=40&md5=9be799d6f4f04f0c138
cdb9ddf2bcb45
VL - 46
ID - 5560
ER -
TY - JOUR
AB - Prostate cancer is one of the most common malignancies among men worldwide.
The main aim of the present work was to clarify the advantages of a nanoformulation
of ayurvedic herbal plants. Specifically, we assessed the improved anticancer
activity of Leucas aspera nanoparticles compared with methanolic crude extract in
PC3 prostate cancer cells and normal cells. L. aspera is a plant that is used in
ayurveda due to the antirheumatic, antipyretic, anti-inflammatory, antibacterial,
anticancer, and cytotoxic activities. Nanoparticles of L. aspera were prepared from
plant methanolic extracts. Cytotoxic effect was studied in the normal and prostate
cancer cells. Size and morphology of the formulated nanoparticles was assessed
using dynamic light scattering and scanning electron microscopy. In vitro
cytotoxicity of L. aspera nanoparticles for PC3 cells was concentration- and time-
dependent. In vitro hemolysis assay, cellular uptake studies, cell aggregation
studies, and cell migration assay established the anticancerous activity of L.
aspera in prostate cancer.
AN - WOS:000399194800011
AU - Mohan, A.
AU - Nair, S. V.
AU - Lakshmanan, V. K.
DA - APR
DO - 10.1007/s12010-016-2291-5
IS - 4
PY - 2017
SN - 0273-2289
1559-0291
SP - 1388-1400
ST - Leucas aspera Nanomedicine Shows Superior Toxicity and Cell Migration
Retarded in Prostate Cancer Cells
T2 - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
TI - Leucas aspera Nanomedicine Shows Superior Toxicity and Cell Migration
Retarded in Prostate Cancer Cells
VL - 181
ID - 6198
ER -
TY - JOUR
AB - The present study deals with the biosynthesis of palladium nanoparticles
(PdNPs) using Agaricus bisporus and exploring its potential biological
applications. The synthesized PdNPs were characterized by UV-visible, FTIR and XRD
techniques. Microscopic analyses revealed the triangular (SEM and AFM) and
spherical (TEM) morphologies of the nanoparticles with nanosize dimension ranging
from 13 to 18 nm. The surface charge of the PdNPs were identified with the help of
zeta potential and found to be negatively charged (- 24.3 mV). The PdNPs exhibited
good antioxidant effect against DPPH free radicals with maximum radical scavenging
activity of 77% using 50 mu g/ml. FTIR spectra of the final DPPH solution depicted
sharp intense signals at 1018 cm(-1) (polysaccharides) and 3342 cm(-1) (phenolic
acids) evidencing the role of these bio functional groups in neutralizing the free
radicals. Antibacterial assay revealed that PdNPs exhibited enhanced growth
inhibition effect against gram positive bacteria (S. auerus; S. pyrogens; B.
subtilis) than gram negative bacterial pathogens (E. aerogenes; K. pneumoniae; P.
vulgaris). Anti-inflammatory activity performed with RBC cells showing 87% of
activity for biosynthesized PdNPs. MTT assay demonstrated that PdNPs exhibited
excellent cytotoxic effect against PK13 cell lines. Maximum growth inhibition of
79% was observed for the maximum dose (50 mu g/ml) with IC50 value of 26.1 mu g/ml.
AN - WOS:000511990800009
AU - Mohana, S.
AU - Sumathi, S.
DA - MAR
DO - 10.1007/s10876-019-01652-2
IS - 2
PY - 2020
SN - 1040-7278
1572-8862
SP - 391-400
ST - Multi-Functional Biological Effects of Palladium Nanoparticles Synthesized
Using Agaricus bisporus
TI - Multi-Functional Biological Effects of Palladium Nanoparticles Synthesized
Using Agaricus bisporus
VL - 31
ID - 6580
ER -
TY - JOUR
AB - BACKGROUND Zinc oxide nanoparticles play a vital role in diagnostics,
biomolecular detection, and microelectronics. Several conventional methods are used
for synthesis of zinc oxide nanoparticles. But, toxic chemicals are required as
capping agents to maintain stability, thus leading to toxicity in the environment.
Thus, we need to shift to "green synthesis". Hence, this study was conducted to
assess the cytotoxicity, antiinflammatory, and antioxidant activity of zinc oxide
nanoparticles reinforced with clove and cinnamon. METHODS Cytotoxic effect, anti-
inflammatory activity, antioxidant activity of zinc oxide nanoparticles reinforced
with clove and cinnamon extract were assessed using Brine Shrimp Assay, Bovine
Serum Albumin (BSA) and DPPH Assay respectively at 5 mu L, 10 mu L, 20 mu L, 30 mu
L, 50 mu L. RESULTS As the concentration increased, the cytotoxicity of the
nanoparticles increased. Values for anti-inflammatory property of nanoparticles was
higher than the standard values at all concentrations. Percentage of inhibition was
highest at 40 mu L (91.1%) and 50 mu L (90.5%). The values for antioxidant property
of nanoparticles was found to be higher than the standard values at all
concentrations except at 50 mu L. Percentage of inhibition was highest at 20 mu L
(86.2%). CONCLUSIONS Zinc oxide nanoparticles reinforced with clove and cinnamon
extract have a potential as an anti-cancer, anti-inflammatory and antioxidant agent
and can be used as an alternative to commercially available products.
AN - WOS:000550748300005
AU - Mohapatra, S.
AU - Leelavathi, L.
AU - Sri Sakthi, D.
AU - Jayashri, P.
DA - JUN 22
DO - 10.14260/jemds/2020/405
IS - 25
PY - 2020
SN - 2278-4748
2278-4802
SP - 1859-1864
ST - Assessment of Cytotoxicity, Anti-Inflammatory and Antioxidant Activity of
Zinc Oxide Nanoparticles Synthesized Using Clove and Cinnamon Formulation - An In-
Vitro Study
T2 - JOURNAL OF EVOLUTION OF MEDICAL AND DENTAL SCIENCES-JEMDS
TI - Assessment of Cytotoxicity, Anti-Inflammatory and Antioxidant Activity of
Zinc Oxide Nanoparticles Synthesized Using Clove and Cinnamon Formulation - An In-
Vitro Study
VL - 9
ID - 5926
ER -
TY - JOUR
AB - Silver was widely used in medicine to treat bacterial infections in the 19th
and early 20th century, up until the discovery and development of the first modern
antibiotics in the 1940s, which were markedly more effective. Since then, every new
antibiotic introduced to the clinic has led to an associated development of drug
resistance. Today, the threat of extensive bacterial resistance to antibiotics has
reignited interest in alternative strategies to treat infectious diseases, with
silver regaining well-deserved renewed attention. Silver ions are highly disruptive
to bacterial integrity and biochemical function, with comparatively minimal
toxicity to mammalian cells. This review focuses on the antimicrobial properties of
silver and their use in synergistic combination therapy with traditional antibiotic
drugs.
AN - WOS:000441681300001
AU - Mohler, J. S.
AU - Sim, W.
AU - Blaskovich, M. A. T.
AU - Cooper, M. A.
AU - Ziora, Z. M.
DA - SEP-OCT
DO - 10.1016/j.biotechadv.2018.05.004
IS - 5
PY - 2018
SN - 0734-9750
1873-1899
SP - 1391-1411
ST - Silver bullets: A new lustre on an old antimicrobial agent
T2 - BIOTECHNOLOGY ADVANCES
TI - Silver bullets: A new lustre on an old antimicrobial agent
VL - 36
ID - 6203
ER -
TY - CONF
AU - Moldwin, R. M.
AU - Sant, G. R.
DB - Scopus
DO - 10.1097/00003081-200203000-00027
ET - 1
KW - Administration, Intravesical
Amitriptyline
Antidepressive Agents, Tricyclic
Autoimmunity
Capsaicin
Cystectomy
Cystitis, Interstitial
Dimethyl Sulfoxide
Humans
Pentosan Sulfuric Polyester
Solvents
Urinary Diversion
Urodynamics
amitriptyline
anesthetic agent
anticonvulsive agent
BCG vaccine
belladonna alkaloid
bupivacaine
capsaicin
carbamazepine
cimetidine
cystistat
dimethyl sulfoxide
gabapentin
gentamicin
heparin
hyaluronic acid
hydroxyzine
hydroxyzine embonate
lidocaine
narcotic agent
oxychlorosene
paroxetine
pentosan polysulfate
resiniferatoxin
serotonin uptake inhibitor
silver nitrate
triamcinolone
venlafaxine
wcs 90
autoimmunity
bladder distension
bladder epithelium
conference paper
constipation
cystectomy
diet restriction
dyspepsia
fatigue
heart palpitation
human
infection
inflammation
interstitial cystitis
laser surgery
liver toxicity
mast cell
neurogenic bladder
neuromodulation
pathophysiology
respiration depression
self care
sexual dysfunction
treatment planning
urinalysis
urinary diversion
urine retention
urticaria
weight gain
xerostomia
PY - 2002
SP - 259-272
ST - Interstitial cystitis: A pathophysiology and treatment update
T2 - Clinical Obstetrics and Gynecology
TI - Interstitial cystitis: A pathophysiology and treatment update
0036193697&doi=10.1097%2f00003081-200203000-
00027&partnerID=40&md5=22c1d378958217de4e48082ee3713b6c
VL - 45
ID - 5794
ER -
TY - JOUR
AB - Hidradenitis suppurativa (HS) is a chronic, recurrent, and inflammatory skin
disease characterized by painful, deep-seated, nodules, abscesses, and sinus tracts
in sensitive areas of the body, including axillary, inguinal, and anogenital
regions. Antibiotics represent the first-line pharmacological treatment of HS
because of their anti-inflammatory properties and antimicrobial effects. This
narrative review summarizes the most significant current issues on the role of
systemic antibiotics in the management of HS, critically analyzing the main limits
of their use (antibiotic resistance and toxicity). Although, in the last decades,
several cytokines have been implicated in the pathomechanism of HS and the research
on the use of novel biologic agents in HS has been intensified, antibiotics remain
a valid therapeutic approach. Future challenges regarding antibiotic therapy in HS
comprise their use in association with biologics in the management of acute flare
or as a bridge therapy to surgery. © 2023 by the authors.
AU - Molinelli, E.
AU - De Simoni, E.
AU - Candelora, M.
AU - Sapigni, C.
AU - Brisigotti, V.
AU - Rizzetto, G.
AU - Offidani, A.
AU - Simonetti, O.
C7 - 978
DB - Scopus
IS - 6
KW - antibiotic resistance
antibiotics
clindamycin
dalbavancin
ertapenem
guidelines
hidradenitis suppurativa rifampicin
tetracycline
adalimumab
ampicillin
antibiotic agent
azithromycin
biological product
C reactive protein
carbapenem
ceftriaxone
chloramphenicol
ciprofloxacin
clarithromycin
colchicine
colistin
corticosteroid
cytokine
daptomycin
DNA topoisomerase (ATP hydrolysing)
doxycycline
epidermal growth factor receptor
erythromycin
etretin
gelatinase B
interleukin 12
interleukin 17
interleukin 6
interleukin 8
lincosamide
linezolid
macrolide
meropenem
metronidazole
minocycline
moxifloxacin
oxazolidinone derivative
rifampicin
silver nanoparticle
teicoplanin
tigecycline
transcriptome
trimethoprim
vancomycin
vasculotropin
abscess
anemia
antibiotic therapy
antifungal activity
bacterial load
biofilm
body mass
bridging therapy
cholestasis
dermatitis
Dermatology Life Quality Index
disease activity
disease free survival
disk diffusion
dysbiosis
dyspepsia
erythema
headache
human
inflammation
latent tuberculosis
liver injury
methicillin susceptible Staphylococcus aureus
neurotoxicity
nonhuman
pain intensity
perineum
pharmacogenomics
practice guideline
protein synthesis
pruritus
pseudomembranous colitis
psoriasis
quality of life
Review
skin disease
suppurative hidradenitis
systemic therapy
Th17 cell
M3 - Review
PY - 2023
ST - Systemic Antibiotic Therapy in Hidradenitis Suppurativa: A Review on
Treatment Landscape and Current Issues
T2 - Antibiotics
TI - Systemic Antibiotic Therapy in Hidradenitis Suppurativa: A Review on
Treatment Landscape and Current Issues
85163743020&doi=10.3390%2fantibiotics12060978&partnerID=40&md5=90a06b994ccda40b5912
ebf6521f8867
VL - 12
ID - 5057
ER -
TY - JOUR
AB - Purpose: This article details the application of pristine nitrogen and sulfur
doped carbon quantum dots (CQDs) as a novel fluorescence biosensor for the
detection of glutathione. The second object of this study is to evaluate reduction
of cellular nitric oxide in microglial cells. Methods: Microwave assisted
hydrothermal method was used for the fabrication of CQDs. Unlike conventional
methods which utilize metallic or transition metal coating over CQDs for the
fabrication of fluorescence switch on/off probes, our simple yet efficient CQDs
itself performed as a biosensor that is both selective and sensitive towards
glutathione (GSH). Particle size analyzer, scanning electron microscope, atomic
force microscopy, high-performance X-ray photoelectron spectroscopy, fourier-
transform infrared spectroscopy were used for physicochemical characterization of
developed CQDs. Photoluminescence properties of CQDs were analyzed using
photoluminescence spectroscope for glutathione detection. Furthermore, microglial
cells were used to evaluate reduction of cellular nitric oxide. Results: The
developed biosensor was able to detect GSH within a short time of 2 min. Hemolysis
assay confirmed negligible red blood cell lysis even at a higher concentration of
0.2 mg/mL. Furthermore, the developed CQDs demonstrated enhanced cellular uptake,
which resulted in generating fluorescence from the BV-2 microglial
cells. Interestingly, the developed CQDs were able to mitigate the secretion of
toxic pro-inflammatory cytokine, nitric oxide (NO) from the lipopolysaccharide
(LPS) insulted BV-2 microglial cells. A 50% reduction in the secretion of NO was
observed after treating with CQDs in the LPS treated BV-2 cells. Conclusion: These
novel fluorescent CQDs with low manufacturing costs, high selectivity and
sensitivity towards GSH and shorter detection time manifest them as a promising
nanomaterial for diverse biomedical applications. © 2020, The Korean Society of
Pharmaceutical Sciences and Technology.
AU - Mondal, J.
AU - Revuri, V.
AU - Choochana, P.
AU - Ganesan, P.
AU - Kang, W. J.
AU - Lee, Y. K.
DB - Scopus
DO - 10.1007/s40005-019-00466-8
IS - 2
KW - Biosensor
Carbon quantum dots
Glutathione
Neuroinflammation
Nitric oxide
acetylcholine
carbon
cystamine
cystine
glutathione
gold nanoparticle
graphene oxide
lead
lipopolysaccharide
nitric oxide
nitrogen
quantum dot
silver nanoparticle
sulfur
zinc
Article
cell viability
controlled study
cytotoxicity
hemolysis assay
human
human cell
limit of detection
microglia
MTT assay
oxidation reduction potential
oxidative stress
particle size
photoluminescence
priority journal
spectral sensitivity
spectrofluorometry
X ray diffraction
M3 - Article
PY - 2020
SP - 209-218
ST - Sulfur and nitrogen doped carbon quantum dots for detection of glutathione
and reduction of cellular nitric oxide in microglial cells
T2 - Journal of Pharmaceutical Investigation
TI - Sulfur and nitrogen doped carbon quantum dots for detection of glutathione
and reduction of cellular nitric oxide in microglial cells
85078160710&doi=10.1007%2fs40005-019-00466-
8&partnerID=40&md5=1faff647c476857a964ca16c42b1dd65
VL - 50
ID - 5288
ER -
TY - JOUR
AB - The aim of this study was to evaluate the antibacterial activity and
cytotoxic effect of the compound SIAB-GV3, a new formulation presenting as an
aqueous suspension of silicon dioxide (SiO2) functionalized with silver (Ag+)
nanoparticles and benzalkonium. The product is formulated as an adjuvant for the
treatment of inflammatory diseases of the oral cavity. This study demonstrates that
SIAB-GV3 possesses strong antimicrobial activity against most of the common oral
pathogens, in particular against Streptococcus pyogenes, Porphyromonas gingivalis
and Aggregatibacter actinimycetemcomitans. The cytotoxic effect against human
embryo lung fibroblasts (HELF cells) was evaluated at different times, from 1 h to
168 h, using concentrations of SIAB-GV3 ranging from 50 mg/ml to 0.0008 mg/ml. At
the concentration of 10 mg/ml, commonly used in clinical practice, the compound
results cytotoxic after about 2 hours, this time being much longer than the typical
time of local application, which is no more than 10 minutes. In conclusion, our
findings suggest that SIAB-GV3 has a good antibacterial activity against the most
common oral pathogens even at very low concentrations and a low cytotoxic activity,
thanks to the synergistic effects of Ag nanoparticles, silicon dioxide and
benzalkonium.
AU - Monzillo, V.
AU - Valle, C. D.
AU - Corbella, M.
AU - Percivalle, E.
AU - Sassera, D.
AU - Scevola, D.
AU - Marone, P.
DB - Scopus
IS - 4
Cytotoxic effect
SIAB-GV3
Bacteria
Cell Line
Cell Survival
Fibroblasts
Humans
Silicon Dioxide
Silver
antiinfective agent
silicon dioxide
silver
cell line
cell survival
drug effects
drug screening
fibroblast
human
toxicity
M3 - Article
PY - 2014
SP - 535-541
ST - Antibacterial activity and cytotoxic effect of SIAB-GV3
T2 - New Microbiologica
TI - Antibacterial activity and cytotoxic effect of SIAB-GV3
84908505602&partnerID=40&md5=e9f22b18bf9bde334c97fd44537ed205
VL - 37
ID - 5608
ER -
TY - GEN
AB - Embora a terapia fotodinâmica venha sendo utilizada como uma ferramenta útil
nos últimos 30 anos em oncologia, poucos estudos clínicos em odontologia têm sido
conduzidos. A terapia fotodinâmica (TFD) utiliza fotossensibilizantes atóxicos e
seletivos que são administrados nas células alvo seguida de aplicação local de luz
visível, produzindo espécies reativas de oxigênio capazes de ocasionar morte
celular por apoptose ou necrose, de afetar a vascularidade local, além de exercer
importantes efeitos no sistema imune. Novas gerações de fármacos
fotossensibilizantes, como as ftalocianinas nanoparticuladas tem apresentado
excelentes resultados na atividade antitumoral e antibacteriana. Neste contexto, o
presente trabalho realizou o primeiro protocolo clínico de aplicação local da
nanoemulsão de cloroalumínio ftalocianina (AlClFc) seguida de irradiação em gengiva
de humanos, e analisou descritiva e comparativamente, por meio de imunoistoquímica,
a expressão de RANK, RANKL, OPG e VEGF em um modelo split-mouth. Oito voluntários
saudáveis com indicação clínica de exodontia foram incluídos no estudo. Sete dias
antes da exodontia, foi aplicado na gengiva dos participantes, 5µM de nanoemulsão
de AlClFc seguida de irradiação com laser diodo (660nm, 7J/cm2), o lado
contralateral foi utilizado como controle. Os espécimes teciduais foram removidos
sete dias após a TFD e subdivididos em dois grupos (grupo teste e grupo controle)
para análise histológica e imunoistoquímica. Os pacientes foram monitorados no dia
aplicação, 7, 14 e 30 dias após a terapia para avaliação de efeitos adversos da
terapia. Alterações vasculares foram observadas nas amostras gengivais que
receberam a TFD. Áreas de edema e congestão vascular, além de intensa
vascularização foram visualizadas. Adicionalmente, focos de calcificação distrófica
em região subepitelial foram visualizados nos espécimes do grupo teste. Os
resultados demonstraram um padrão similar dos escores de imunomarcação de RANK,
RANKL e VEGF entre os grupos teste e controle, não havendo diferença estatística
significante (p=0.317, p=0.777, p=0.814, respectivamente). RANK e RANKL exibiram
imunomarcação fraca ou ausente na maioria dos espécimes analisados. Não houve
imunomarcação para a OPG. O VEGF mostrou imunomarcação moderada a forte nos
espécimes do grupo teste. Adicionalmente, o estudo clínico mostrou que a terapia
foi bem tolerada por todos os pacientes. Os efeitos adversos foram de curta duração
e totalmente reversíveis. Tomados em conjunto, os resultados apresentados neste
estudo mostraram que o protocolo utilizado por nós, mediado por nanoemulsão
contendo AlClFc, é seguro para aplicação clínica em tecido gengival e, sugerem uma
forte imunomarcação para o VEGF após a terapia. (AU)
Although photodynamic therapy have been used as a useful tool over the past 30
years in oncology, few clinical trials have been conducted in dentistry.
Photodynamic therapy (PDT) uses non-toxic photosensitizers and selective which are
administered in target cells followed by local application of visible light,
producing reactive oxygen species capable of causing cell death by apoptosis or
necrosis, injured the local vasculature, and exert important effects on the immune
system. New generations of photosensitizing agents, such as nanoparticulate
phthalocyanines, has shown excellent results in antitumor and antibacterial
activity . In this context, the present work constitutes the first clinical
protocol of local application of nanoemulsion chloro-aluminum phthalocyanine
(AlClFc) followed by irradiation in human gingiva, and analyzed descriptively and
comparatively , by means of immunohistochemistry , the expression of RANK , RANKL ,
OPG and VEGF in a split -mouth model. Eight healthy volunteers with clinical
indication for extraction were included in the study . Seven days before the
extraction, was injected in the gingiva of participants, 5µM of nanoemulsion AlClFc
followed by irradiation with diode laser (660nm, 7J/cm2 ), the contralateral side
was used as control. Tissue specimens were removed seven days after the TFD is
performed. Tissues sample were divided into two groups (test and control groups)
for histological and immunohistochemical analysis. Patients were monitored at days,
0, 7, 14 and 30 to assess adverse effects of the therapy. Vascular alterations were
seen in gingival samples that received PDT. Areas of edema and vascular congestion,
and intense vascularization were viewed . Additionally, dystrophic calcification in
subepithelial region were observed in the test group. The results showed a similar
pattern of immunostaining scores of RANK, RANKL and VEGF between the test and
control groups, with no statistically significant difference (p = 0.317, p = 0.777,
p = 0.814, respectively). RANK and RANKL exhibited weak or absent immunostaining in
most specimens analyzed. There was no immunostaining for OPG. VEGF showed moderate
to strong immunostaining in specimens from the test group. In addition, the
clinical study showed that therapy was well tolerated by all patients. Adverse
effects were short-time and completely reversible. Taken together, the results
presented in this study showed that PDT mediated by nanoemulsion containing AlClPc
is safe for clinical application in gingival tissue and suggests that a strong
immunostaining for VEGF after therapy. (AU)
AU - Moraes, Maiara de
C1 - 20140801
C8 - biblio-867010
DA - 2014/08
DB - LILACS
KW - Fator A de crescimento do endotélio vascular
Fotoquimioterapia
Fármacos fotossensibilizantes
Ligante RANK
Osteoprotegerin
Osteoprotegerina
Photochemotherapy
Photosensitizing agents
RANK ligand
Vascular endothelial growth factor A
LA - pt
PY - 2014
SP - 128-128
ST - Análise imunoistoquímica após terapia fotodinâmica com cloro-alumínio
ftalocianina em tecidos gengivais de humanos
TI - Análise imunoistoquímica após terapia fotodinâmica com cloro-alumínio
ftalocianina em tecidos gengivais de humanos
TT - Immunohistochemical analyzes after photodynamic therapy with chloro-aluminum
phthalocyanine in human gingival tissues
UR - https://pesquisa.bvsalud.org/portal/resource/pt/biblio-867010
ID - 4939
ER -
TY - JOUR
AB - Bacterial endotoxins are a component of particulate matter (PM) with
anticipated health implications, yet we know little about how host reception of
endotoxin through toll-like receptor 4 (TLR4) is affected by its association with
other PM components. Subsequently, we investigated the relationship between
endotoxin concentration (recombinant Factor C (rFC) assay) and host recognition
(HEK Blue-TLR4 NF-kB reporter cell line based assay) in various compositions of
urban PM, including road traffic, industrial and urban green land use classes.
While the assays did not correlate strongly between each other, the TLR4 reporter
cell line was found to be better correlated to the IL-8 response of PM.
Furthermore, the ability of the quantified endotoxin (rFC assay) to stimulate the
TLR4/MD-2 complex was significantly affected by the urban land use class, where
traffic locations were found to be significantly higher in bioactive endotoxin than
the industrial and green locations. We subsequently turned our attention to PM
composition and characterized the samples based on transition metal content
(through ICP-MS). The effect of nickel and cobalt – previously reported to activate
the hTLR4/MD-2 complex – was found to be negligible in comparison to that of iron.
Here, the addition of iron as a factor significantly improved the regression model
between the two endotoxin assays, explaining 77% of the variation of the TLR4
stimulation and excluding the significant effect of land use class. Moreover, the
effect of iron proved to be more than a correlation, since dosing LPS with Fe2+ led
to an increase up to 64% in TLR4 stimulation, while Fe2+ without LPS was unable to
stimulate a response. This study shows that endotoxin quantification assays (such
as the rFC assay) may not always correspond to human biological recognition of
endotoxin in urban PM, while its toxicity can be synergistically influenced by the
associated PM composition. Although endotoxins are typically quantified in ambient
air, its toxicity is significantly influenced by the associated PM composition
(e.g. as shown in this study with iron, one of the most abundant transition metals
found in urban PM). © 2018
AU - Moretti, S.
AU - Smets, W.
AU - Hofman, J.
AU - Mubiana, K. V.
AU - Oerlemans, E.
AU - Vandenheuvel, D.
AU - Samson, R.
AU - Blust, R.
AU - Lebeer, S.
DB - Scopus
DO - 10.1016/j.envpol.2018.09.148
KW - Inflammation
Lipopolysaccharide
Particulate matter
TLR4
Transition metals
Biological Assay
Cell Line
Endotoxins
Humans
Interleukin-8
Metals
Particulate Matter
Transition Elements
Cell culture
Iron
Land use
Regression analysis
Toxicity
aluminum
arsenic
cadmium
calcium
chloride
chromium
cobalt
copper
endotoxin
interleukin 8
iron
lead
lipopolysaccharide
manganese
nickel
protein MD 2
silver
transition element
zinc
metal
TLR4 protein, human
Bacterial endotoxins
Biological recognition
Lipopolysaccharides
Quantification assays
bacterium
particulate matter
polysaccharide
protein
toxin
Article
cell assay
human
industrial area
land use
traffic
bioassay
cell line
drug effect
metabolism
statistics and numerical data
toxicity
Assays
M3 - Article
PY - 2019
SP - 118-126
ST - Human inflammatory response of endotoxin affected by particulate matter-bound
transition metals
T2 - Environmental Pollution
TI - Human inflammatory response of endotoxin affected by particulate matter-bound
transition metals
85054748150&doi=10.1016%2fj.envpol.2018.09.148&partnerID=40&md5=e602dc4a5dad70aa5ba
a59e7bd782b81
VL - 244
ID - 5441
ER -
TY - JOUR
AB - Nanoparticles can interact with the complement system and modulate the
inflammatory response. The effect of these interactions on the complement activity
strongly depends on physicochemical properties of nanoparticles. The interactions
of silver nanoparticles with serum proteins (particularly with the complement
system components) have the potential to significantly affect the antibacterial
activity of serum, with serious implications for human health. The aim of the study
was to assess the influence of graphite oxide (GO) nanocomposites (GO, GO‐
PcZr(Lys)2‐Ag, GO‐Ag, GO‐PcZr(Lys)2) on the antibacterial activity of normal human
serum (NHS), serum activity against bacteria isolated from alveoli treated with
nanocomposites, and nanocomposite sensitivity of bacteria exposed to serum in vitro
(using normal human serum). Additionally, the in vivo cytotoxic effect of the GO
compounds was determined with application of a Galleria mellonella larvae model.
GO‐PcZr(Lys)2, without IR irradiation enhance the antimicrobial efficacy of the
human serum. IR irradiation enhances bactericidal activity of serum in the case of
the GO‐PcZr(Lys)2‐Ag sample. Bacteria exposed to nanocomposites become more
sensitive to the action of serum. Bacteria exposed to serum become more sensitive
to the GO‐Ag sample. None of the tested GO nanocomposites displayed a cytotoxicity
towards larvae. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Morka, K. D.
AU - Wernecki, M.
AU - Kędziora, A.
AU - Książczyk, M.
AU - Dudek, B.
AU - Gerasymchuk, Y.
AU - Lukowiak, A.
AU - Bystroń, J.
AU - Bugla‐płoskońska, G.
C7 - 7386
DB - Scopus
DO - 10.3390/ijms22147386
IS - 14
KW - Bactericidal action of serum
E. coli
GO nanocomposites
Photoactivity
Animals
Cell Survival
Escherichia coli
Graphite
Humans
Infrared Rays
Larva
Lepidoptera
Metal Nanoparticles
Nanocomposites
Oxides
Serum
Silver
graphite
nanocomposite
oxide
phthalocyanine
silver nanoparticle
zirconium derivative
antiinfective agent
metal nanoparticle
silver
animal experiment
Article
bacterial survival
controlled study
cytotoxicity
Galleria mellonella
human
in vitro study
in vivo study
larva
lung alveolus
nonhuman
serum bactericidal activity
animal
cell survival
chemistry
drug effect
infrared radiation
microbiology
radiation response
serum
M3 - Article
PY - 2021
ST - The impact of graphite oxide nanocomposites on the antibacterial activity of
serum
TI - The impact of graphite oxide nanocomposites on the antibacterial activity of
serum
85109333277&doi=10.3390%2fijms22147386&partnerID=40&md5=359defe7bcff01b0fb5403d7322
22d97
VL - 22
ID - 5220
ER -
TY - JOUR
AB - In this study, we explored the effects of a longer term application, up to
12 weeks, of photobiomodulation in normal, naïve macaque monkeys. Monkeys (n = 5)
were implanted intracranially with an optical fibre device delivering
photobiomodulation (red light, 670 nm) to a midline midbrain region. Animals were
then aldehyde-fixed and their brains were processed for immunohistochemistry. In
general, our results showed that longer term intracranial application of
photobiomodulation had no adverse effects on the surrounding brain parenchyma or on
the nearby dopaminergic cell system. We found no evidence for photobiomodulation
generating an inflammatory glial response or neuronal degeneration near the implant
site; further, photobiomodulation did not induce an abnormal activation or
mitochondrial stress in nearby cells, nor did it cause an abnormal arrangement of
the surrounding vasculature (endothelial basement membrane). Finally, because of
our interest in Parkinson’s disease, we noted that photobiomodulation had no impact
on the number of midbrain dopaminergic cells and the density of their terminations
in the striatum. In summary, we found no histological basis for any major biosafety
concerns associated with photobiomodulation delivered by our intracranial approach
and our findings set a key template for progress onto clinical trial on patients
with Parkinson’s disease. © 2017, Springer-Verlag GmbH Germany.
AU - Moro, C.
AU - Torres, N.
AU - Arvanitakis, K.
AU - Cullen, K.
AU - Chabrol, C.
AU - Agay, D.
AU - Darlot, F.
AU - Benabid, A. L.
AU - Mitrofanis, J.
DB - Scopus
DO - 10.1007/s00221-017-5048-7
IS - 10
KW - 670 nm
Behaviour
Macaque monkeys
Striatum
Substantia nigra
Tyrosine hydroxylase
Animals
Corpus Striatum
Dopaminergic Neurons
Low-Level Light Therapy
Macaca fascicularis
Mesencephalon
Optical Fibers
chaperonin 60
IBA1 protein
protein
protein fos
unclassified drug
glass fiber
adult
animal cell
animal tissue
antibody labeling
Article
basement membrane
cell damage
controlled study
corpus striatum
cytoarchitecture
endothelial basement membrane
immunoreactivity
mesencephalon
microglia
nerve cell degeneration
Nissl staining
nonhuman
parenchyma
Parkinson disease
photobiomodulation
priority journal
silver impregnation
thalamus
adverse device effect
animal
devices
M3 - Article
PY - 2017
SP - 3081-3092
ST - No evidence for toxicity after long-term photobiomodulation in normal non-
human primates
T2 - Experimental Brain Research
TI - No evidence for toxicity after long-term photobiomodulation in normal non-
human primates
85025812162&doi=10.1007%2fs00221-017-5048-
7&partnerID=40&md5=afa24af1b72ce9ac938d5cdcc0ac6246
VL - 235
ID - 5500
ER -
TY - JOUR
AB - Nanosilver with sizes 1–100 nm at least in one dimension is widely used due
to physicochemical, anti-inflammatory, anti-angiogenesis, antiplatelet, antifungal,
anticancer, antibacterial, and antiviral properties. Three modes of the nanosilver
action were suggested: “Trojan horse”, inductive, and quantum mechanical. The Ag+
cations have an affinity to thiol, amino, phosphate, and carboxyl groups. Multiple
mechanisms of action towards proteins, DNA, and membranes reduce a risk of pathogen
resistance but inevitably cause toxicity for cells and organisms. Silver
nanoparticles (AgNP) are known to generate two reactive oxygen species (ROS)-
superoxide (•O2−) and hydroxyl (•OH) radicals, which inhibit the cellular
antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase)
and cause mechanical damage of membranes. Ag+ release and replacement by
electrolyte ions with potential formation of insoluble AgCl result in NP
instability and interactions of heavy metals with nucleic acids and proteins.
Protein shells protect AgNP core from oxidation, dissolution, and aggregation, and
provide specific interactions with ligands. These nanoconjugates can be used for
immunoassays and diagnostics, but the sensitivity is limited at 10 pg and
specificity is restricted by binding with protective proteins (immunoglobulins,
fibrinogen, albumin, and others). Thus, broad implementation of Ag nanostructures
revealed limitations such as instability; binding with major blood proteins; damage
of proteins, nucleic acids, and membranes; and immunosuppression of the majority of
cytokines. © 2021 by the author. Licensee MDPI, Basel, Switzerland.
AU - Morozova, O. V.
C7 - 9928
DB - Scopus
DO - 10.3390/ijms22189928
IS - 18
KW - Cytotoxicity
Immunosupression
Nanoconjugates with silver core and protein shells
Nanopaticles
Silver ions
Stability
Animals
Cytoprotection
Humans
Limit of Detection
Metal Nanoparticles
Silver
Toxicity Tests
citric acid
nanoconjugate
protein
silver nanoparticle
metal nanoparticle
silver
host resistance
human
immunomodulation
nonhuman
protection
quantum mechanics
Review
toxicity testing
animal
cell protection
drug effect
limit of detection
M3 - Review
PY - 2021
ST - Silver nanostructures: Limited sensitivity of detection, toxicity and anti-
inflammation effects
TI - Silver nanostructures: Limited sensitivity of detection, toxicity and anti-
inflammation effects
85114796464&doi=10.3390%2fijms22189928&partnerID=40&md5=abd1f6c1cc13d46f7c1bc6959fc
27f65
VL - 22
ID - 5216
ER -
TY - JOUR
AB - Background: Nanosilver possesses antiviral, antibacterial, anti-inflammatory,
anti-angio-genesis, antiplatelet, and anticancer properties. The development of
disinfectants, inactivated vac-cines, and combined etiotropic and immunomodulation
therapy against respiratory viral infections, including COVID-19, remains urgent.
Aim: Our goal was to determine the SARS-CoV-2 molecular targets (genomic RNA and
the structural virion proteins S and N) for silver-containing nanomateri-als.
Methods: SARS-CoV-2 gene cloning, purification of S2 and N recombinant proteins,
viral RNA isolation from patients’ blood samples, reverse transcription with
quantitative real-time PCR ((RT)2-PCR), ELISA, and multiplex immunofluorescent
analysis with magnetic beads (xMAP) for detection of 17 inflammation markers.
Results: Fluorescent Ag nanoclusters (NCs) less than 2 nm with a few recovered
silver atoms, citrate coated Ag nanoparticles (NPs) with diameters of 20–120 nm,
and nanoconjugates of 50–150 nm consisting of Ag NPs with different protein
envelopes were con-structed from AgNO3 and analyzed by means of transmission
electron microscopy (TEM), atomic force microscopy (AFM), ultraviolet-visible light
absorption, and fluorescent spectroscopy. SARS-CoV-2 RNA isolated from COVID-19
patients’ blood samples was completely cleaved with the artificial RNase complex
compound Li+[Ag+2Cys2−(OH−)2(NH3)2] (Ag-2S), whereas other Ag-contain-ing materials
provided partial RNA degradation only. Treatment of the SARS-CoV-2 S2 and N re-
combinant antigens with AgNO3 and Ag NPs inhibited their binding with specific
polyclonal anti-bodies, as shown by ELISA. Fluorescent Ag NCs with albumin or
immunoglobulins, Ag-2S com-plex, and nanoconjugates of Ag NPs with protein shells
had no effect on the interaction between coronavirus recombinant antigens and
antibodies. Reduced production of a majority of the 17 inflammation biomarkers
after treatment of three human cell lines with nanosilver was demonstrated by xMAP.
Conclusion: The antiviral properties of the silver nanomaterials against SARS-CoV-2
coronavirus differed. The small-molecular-weight artificial RNase Ag-2Sprovided
exhaustive RNA destruction but could not bind with the SARS-CoV-2 recombinant
antigens. On the contrary, Ag+ ions and Ag NPs interacted with the SARS-CoV-2
recombinant antigens N and S but were less efficient at performing viral RNA
cleavage. One should note that SARS-CoV-2 RNA was more stable than MS2 phage RNA.
The isolated RNA of both the MS2 phage and SARS-CoV-2 were more de-gradable than
the MS2 phage and coronavirus particles in patients’ blood, due to the protection
with structural proteins. To reduce the risk of the virus resistance, a combined
treatment of Ag-2S with Ag NPs could be used. To prevent cytokine storm during the
early stages of respiratory infections with RNA-containing viruses, nanoconjugates
of Ag NPs with surface proteins could be used. © 2022 by the authors. Licensee
AU - Morozova, O. V.
AU - Manuvera, V. A.
AU - Grishchechkin, A. E.
AU - Barinov, N. A.
AU - Shevlyagina, N. V.
AU - Zhukhovitsky, V. G.
AU - Lazarev, V. N.
AU - Klinov, D. V.
C7 - 902
DB - Scopus
DO - 10.3390/v14050902
IS - 5
KW - beta-coronavirus
ELISA
nanosilver
RNA-containing bacteriophage MS2
RT2-PCR
xMAP
Antiviral Agents
Cations
COVID-19
Cystine
Humans
Inflammation
Metal Nanoparticles
Nanoconjugates
Ribonucleases
RNA, Viral
SARS-CoV-2
Silver
Virion
coronavirus nucleocapsid protein
gamma interferon
interleukin 10
interleukin 12p70
interleukin 13
interleukin 17
interleukin 1beta
interleukin 2
interleukin 4
interleukin 5
interleukin 6
interleukin 7
interleukin 8
metal complex
nanoconjugate
silver
silver cystine complex
silver nanocluster
silver nanoparticle
silver nitrate
unclassified drug
virus RNA
vitronectin
antivirus agent
cation
cystine
metal nanoparticle
recombinant protein
ribonuclease
adult
analytic method
Article
controlled study
cytokine storm
cytotoxicity
female
fluorescent spectroscopy
HEp-2 cell line
HT-29 cell line
human
human cell
human tissue
immunofluorescence
male
molecular cloning
MTT assay
multiplex immunofluorescence
nasopharyngeal swab
particle size
plasmid
protein purification
real time reverse transcription polymerase chain reaction
RNA isolation
Sanger sequencing
virion
chemistry
genetics
inflammation
M3 - Article
PY - 2022
ST - Targeting of Silver Cations, Silver-Cystine Complexes, Ag Nanoclusters, and
Nanoparticles towards SARS-CoV-2 RNA and Recombinant Virion Proteins
T2 - Viruses
TI - Targeting of Silver Cations, Silver-Cystine Complexes, Ag Nanoclusters, and
Nanoparticles towards SARS-CoV-2 RNA and Recombinant Virion Proteins
85129859965&doi=10.3390%2fv14050902&partnerID=40&md5=56109e4ea70eceb17ce216bc79b986
aa
VL - 14
ID - 5092
ER -
TY - JOUR
AB - E-cigarettes utilize a wide range of flavoring chemicals with respiratory
health effects that are not well understood. In this study, we used pulmonary-
associated cell lines to assess the in vitro cytotoxic effects of 30 flavoring
chemicals. Human bronchial epithelial cells (BEAS-2B) and both naive and activated
macrophages (THP-1) were treated with 10, 100, and 1000 mu M of flavoring chemicals
and analyzed for changes in viability, cell membrane damage, reactive oxygen
species (ROS) production, and inflammatory cytokine release. Viability was
unaffected for all chemicals at the 10 and 100 mu M concentrations. At 1000 mu M,
the greatest reductions in viability were seen with decanal, hexanal, nonanal,
cinnamaldehyde, eugenol, vanillin, alpha-pinene, and limonene. High amounts of ROS
were elicited by vanillin, ethyl maltol, and the diketones (2,3-pentanedione, 2,3-
heptanedione, and 2,3-hexanedione) from both cell lines. Naive THP-1 cells produced
significantly elevated levels of IL-1 beta, IL-8, and TNF-alpha when exposed to
ethyl maltol and hexanal. Activated THP-1 cells released increased IL-1 beta and
TNF-alpha when exposed to ethyl maltol, but many flavoring chemicals had an
apparent suppressive effect on inflammatory cytokines released by activated
macrophages, some with varying degrees of accompanying cytotoxicity. The diketones,
L-carvone, and linalool suppressed cytokine release in the absence of cytotoxicity.
These findings provide insight into lung cell cytotoxicity and inflammatory
cytokine release in response to flavorings commonly used in e-cigarettes.
AN - WOS:000720040000001
AU - Morris, A. M.
AU - Leonard, S. S.
AU - Fowles, J. R.
AU - Boots, T. E.
AU - Mnatsakanova, A.
AU - Attfield, K. R.
C7 - 11107
DA - NOV
DO - 10.3390/ijerph182111107
IS - 21
PY - 2021
SN - 1660-4601
ST - Effects of E-Cigarette Flavoring Chemicals on Human Macrophages and Bronchial
Epithelial Cells
TI - Effects of E-Cigarette Flavoring Chemicals on Human Macrophages and Bronchial
Epithelial Cells
VL - 18
ID - 6400
ER -
TY - JOUR
AB - Respiratory syncytial virus (RSV) is an important etiological agent of
respiratory infection in children for which no specific treatment option is
available. The RSV virion contains two surface glycoproteins (F and G) that are
vital for the initial phases of infection, making them critical targets for RSV
therapeutics. Recent studies have identified the broad-spectrum antiviral
properties of silver nanoparticles (AgNPs) against respiratory pathogens, such as
adenovirus, parainfluenza, and influenza. AgNPs achieve this by attaching to viral
glycoproteins, blocking entry into the host cell. The objective of this study was
to evaluate the antiviral and immunomodulatory effects of AgNPs in RSV infection.
Herein we demonstrate AgNP-mediated reduction in RSV replication, both in
epithelial cell lines and in experimentally infected BALB/c mice. Marked reduction
in pro-inflammatory cytokines (i.e., IL-1α, IL-6, TNF-α) and pro-inflammatory
chemokines (i.e., CCL2, CCL3, CCL5) was also observed. Conversely, CXCL1, G-CSF,
and GM-CSF were increased in RSV-infected mice treated with AgNPs, consistent with
an increase of neutrophil recruitment and activation in the lung tissue. Following
experimental antibody-dependent depletion of neutrophils, the antiviral effect of
AgNPs in mice treated was ablated. To our knowledge, this is the first in vivo
report demonstrating antiviral activity of AgNPs during RSV infection. © 2019 by
the authors. Licensee MDPI, Basel, Switzerland.
AU - Morris, D.
AU - Ansar, M.
AU - Speshock, J.
AU - Ivanciuc, T.
AU - Qu, Y.
AU - Casola, A.
AU - Garofalo, R.
C7 - 732
DB - Scopus
DO - 10.3390/v11080732
IS - 8
KW - AgNP
Anti-Ly6G
Antiviral
Epithelial cells
G-CSF
GM-CSF
KC
Neutrophils
Respiratory syncytial virus
Animals
Antiviral Agents
Cell Line
Cytokines
Epithelial Cells
Female
Humans
Immunologic Factors
Leukocyte Count
Lung
Metal Nanoparticles
Mice
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Human
Silver
Virus Replication
alpha interferon
beta interferon
CXCL1 chemokine
elastase
interleukin 10
interleukin 12p40
interleukin 12p70
interleukin 13
interleukin 1alpha
interleukin 6
interleukin 8
interleukin 9
povidone
protein
RANTES
silver nanoparticle
antivirus agent
cytokine
immunologic factor
metal nanoparticle
silver
A-549 cell line
airway obstruction
animal experiment
animal model
animal tissue
antiviral activity
Article
body plethysmography
body weight loss
colorimetry
controlled study
cytotoxicity test
enzyme activity
female
HEp-2 cell line
human
human cell
immunomodulation
mouse
neutrophil
nonhuman
peak expiratory flow
peak inspiratory flow
respiratory syncytial virus infection
viral plaque assay
virus replication
animal
cell line
chemistry
cytology
disease model
drug effect
epithelium cell
Human respiratory syncytial virus
immunology
leukocyte count
lung
metabolism
pathology
virology
M3 - Article
PY - 2019
ST - Antiviral and immunomodulatory activity of silver nanoparticles in
experimental rsv infection
T2 - Viruses
TI - Antiviral and immunomodulatory activity of silver nanoparticles in
experimental rsv infection
85070476559&doi=10.3390%2fv11080732&partnerID=40&md5=c6c9b588c7cd8465fe017350c1c423
4b
VL - 11
ID - 5455
ER -
TY - JOUR
AB - Nanotechnology is a growing science that may provide several new applications
for medicine, food preservation, diagnostic technologies, and sanitation. Despite
its beneficial applications, there are several questions related to the safety of
nanomaterials for human use. The development of nanotechnology is associated with
some concerns because of the increased risk of carcinogenesis following exposure to
nanomaterials. The increased levels of reactive oxygen species (ROS) that are due
to exposure to nanoparticles (NPs) are primarily responsible for the genotoxicity
of metal NPs. Not all, but most metal NPs are able to directly produce free
radicals through the release of metal ions and through interactions with water
molecules. Furthermore, the increased production of free radicals and the cell
death caused by metal NPs can stimulate reduction/oxidation (redox) reactions,
leading to the continuous endogenous production of ROS in a positive feedback loop.
The overexpression of inflammatory mediators, such as NF-kB and STATs, the
mitochondrial malfunction and the increased intracellular calcium levels mediate
the chronic oxidative stress that occurs after exposure to metal NPs. In this
paper, we review the genotoxicity of different types of metal NPs and the redox
mechanisms that amplify the toxicity of these NPs. © 2019
AU - Mortezaee, K.
AU - Najafi, M.
AU - Samadian, H.
AU - Barabadi, H.
AU - Azarnezhad, A.
AU - Ahmadi, A.
C7 - 108814
DB - Scopus
DO - 10.1016/j.cbi.2019.108814
KW - DNA damage
Genotoxicity
Metal nanoparticles
Nanotechnology
Oxidative stress
Redox
Animals
Calcium
DNA Damage
Food Additives
Humans
Metal Nanoparticles
Neoplasms
Oxidation-Reduction
Oxidative Stress
aluminum nanoparticle
cobalt nanoparticle
copper nanoparticle
cosmetic
food additive
gold nanoparticle
iron nanoparticle
nanoparticle
nickel nanoparticle
palladium nanoparticle
silver nanoparticle
unclassified drug
calcium
metal nanoparticle
antifungal activity
calcium homeostasis
cancer risk
cancer therapy
carcinogenesis
cytotoxicity
genotoxicity
human
inflammation
ion transport
lotion
molecular imaging
nonhuman
Review
teratogenesis
animal
chemistry
drug effect
metabolism
neoplasm
oxidative stress
M3 - Review
PY - 2019
ST - Redox interactions and genotoxicity of metal-based nanoparticles: A
comprehensive review
TI - Redox interactions and genotoxicity of metal-based nanoparticles: A
comprehensive review
85071976190&doi=10.1016%2fj.cbi.2019.108814&partnerID=40&md5=98b7bd457c778d2f2d05d2
782ecead36
VL - 312
ID - 5424
ER -
TY - JOUR
AB - Hydroxyapatite has been extensively used in tissue engineering due to its
osteogenic potency, but its present toxicological facts are relatively
insufficient. Here, the possible gastric toxicity of hydroxyapatite nanoparticles
was evaluated biochemically to determine oxidant and antioxidant parameters in
rats' stomach tissues. At results, hydroxyapatite nanoparticles have declined
stomach antioxidant enzymes and reduced glutathione level, while an induction in
lipid peroxidation and nitric oxide has been observed. Furthermore, DNA oxidation
was analyzed by the suppression of toll-like receptors 2, nuclear factor-kappa B
and Forkhead box P3 gene expression and also 8-Oxo-2'-deoxyguanosine level as a
genotoxicity indicator. Various pro-inflammatory gene products have been identified
that intercede a vital role in proliferation and apoptosis suppression, among these
products: tumor suppressor p53, tumor necrosis factor-alpha and interliukin-6.
Moreover, the hydroxyapatite-treated group revealed wide histological alterations
and significant elevation in the number of proliferating cell nuclear antigen-
positive cells, which has been observed in the mucosal layer of the small
intestine, and these alterations are an indication of small intestine injury, while
the appearance of chitosan and curcumin nanoparticles in the combination group
showed improvement in all the above parameters with inhibition of toxic-oxidant
parameters and activation of antioxidant parameters.
AN - WOS:000604102200018
AU - Mosa, I. F.
AU - Abd, H. H.
AU - Abuzreda, A.
AU - Assaf, N.
AU - Yousif, A. B.
DA - JUL
DO - 10.1093/toxres/tfaa054
IS - 4
PY - 2020
SN - 2045-452X
2045-4538
SP - 493-508
ST - Bio-evaluation of the role of chitosan and curcumin nanoparticles in
ameliorating genotoxicity and inflammatory responses in rats' gastric tissue
followed hydroxyapatite nanoparticles' oral uptake
TI - Bio-evaluation of the role of chitosan and curcumin nanoparticles in
ameliorating genotoxicity and inflammatory responses in rats' gastric tissue
followed hydroxyapatite nanoparticles' oral uptake
VL - 9
ID - 6299
ER -
TY - JOUR
AB - The p63 gene is a member of the p53/p63/p73 family of transcription factors
and plays a critical role in development and homeostasis of squamous epithelium.
p63 is transcribed as multiple isoforms; Delta Np63 alpha, the predominant p63
isoform in stratified squamous epithelium, is localized to the basal cells and is
overexpressed in squamous cell cancers of multiple organ sites, including skin,
head and neck, and lung. Further, p63 is considered a stem cell marker, and within
the epidermis, Delta Np63 alpha directs lineage commitment. Delta Np63 alpha has
been implicated in numerous processes of skin biology that impact normal epidermal
homeostasis and can contribute to squamous cancer pathogenesis by supporting
proliferation and survival with roles in blocking terminal differentiation,
apoptosis, and senescence, and influencing adhesion and migration. Delta Np63 alpha
overexpression may also influence the tissue microenvironment through remodeling of
the extracellular matrix and vasculature, as well as by enhancing cytokine and
chemokine secretion to recruit pro-inflammatory infiltrate. This review focuses on
the role of Delta Np63 alpha in normal epidermal biology and how dysregulation can
contribute to cutaneous squamous cancer development, drawing from knowledge also
gained by squamous cancers from other organ sites that share p63 overexpression as
a defining feature.
AN - WOS:000480449300214
AU - Moses, M. A.
AU - George, A. L.
AU - Sakakibara, N.
AU - Mahmood, K.
AU - Ponnamperuma, R. M.
AU - King, K. E.
AU - Weinberg, W. C.
C7 - 3590
DA - JUL 2
DO - 10.3390/ijms20143590
IS - 14
PY - 2019
SN - 1422-0067
ST - Molecular Mechanisms of p63-Mediated Squamous Cancer Pathogenesis
TI - Molecular Mechanisms of p63-Mediated Squamous Cancer Pathogenesis
VL - 20
ID - 6729
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NPs) are among the most widely used metal-based
nanomaterials (NMs) and their applications in different products, also as
antibacterial additives, are increasing. In the present manuscript, according to an
adverse outcome pathway (AOP) approach, we tested two safe-by-design (SbD) newly
developed Ag NPs coated with hydroxyethyl cellulose (HEC), namely AgHEC powder and
AgHEC solution. These novel Ag NPs were compared to two reference Ag NPs (naked and
coated with polyvinylpyrrolidone—PVP). Cell viability, inflammatory response,
reactive oxygen species, oxidative DNA damage, cell cycle, and cell–particle
interactions were analyzed in the alveolar in vitro model, A549 cells. The results
show a different toxicity pattern of the novel Ag NPs compared to reference NPs and
that between the two novel NPs, the AgHEC solution is the one with the lower
toxicity and to be further developed within the SbD framework. © 2023 by the
authors.
AU - Motta, G.
AU - Gualtieri, M.
AU - Saibene, M.
AU - Bengalli, R.
AU - Brigliadori, A.
AU - Carrière, M.
AU - Mantecca, P.
C7 - 195
DB - Scopus
DO - 10.3390/toxics11020195
IS - 2
KW - adverse outcomes pathway
in vitro lung cells
nano-enabled products
nanotoxicity
safe-by-design
silver nanoparticle hazard
carbon nanotube
histone H2AX
hydroxyethylcellulose
povidone
silver nanoparticle
A-549 cell line
acute toxicity
adverse outcome pathway
Article
biological activity
cell cycle
cell cycle arrest
cell viability
coating (procedure)
controlled study
DNA damage
human
human cell
in vitro study
lung fibrosis
outcome assessment
oxidative stress
powder
preliminary data
surface charge
M3 - Article
PY - 2023
ST - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
Pathway-Driven Approach on Different Silver Nanoparticles: Assessment of Acute
Responses in A549 Cells
T2 - Toxics
TI - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
85149218507&doi=10.3390%2ftoxics11020195&partnerID=40&md5=89bf1266c60e43a2290744133
195be95
VL - 11
ID - 5006
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NPs) are among the most widely used metal-based
nanomaterials (NMs) and their applications in different products, also as
antibacterial additives, are increasing. In the present manuscript, according to an
adverse outcome pathway (AOP) approach, we tested two safe-by-design (SbD) newly
developed Ag NPs coated with hydroxyethyl cellulose (HEC), namely AgHEC powder and
AgHEC solution. These novel Ag NPs were compared to two reference Ag NPs (naked and
coated with polyvinylpyrrolidone-PVP). Cell viability, inflammatory response,
reactive oxygen species, oxidative DNA damage, cell cycle, and cell-particle
interactions were analyzed in the alveolar in vitro model, A549 cells. The results
show a different toxicity pattern of the novel Ag NPs compared to reference NPs and
that between the two novel NPs, the AgHEC solution is the one with the lower
toxicity and to be further developed within the SbD framework.
AN - WOS:000941340400001
AU - Motta, G.
AU - Gualtieri, M.
AU - Saibene, M.
AU - Bengalli, R.
AU - Brigliadori, A.
AU - Carriere, M.
AU - Mantecca, P.
C7 - 195
DA - FEB
DO - 10.3390/toxics11020195
IS - 2
PY - 2023
SN - 2305-6304
ST - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
T2 - TOXICS
TI - Preliminary Toxicological Analysis in a Safe-by-Design and Adverse Outcome
VL - 11
ID - 6089
ER -
TY - JOUR
AB - Zinc nanostructures (ZnONSs) have attracted much attention due to their
morphological, physicochemical, and electrical properties, which were entailed for
various biomedical applications such as cancer and diabetes treatment, anti-
inflammatory activity, drug delivery. ZnONS play an important role in inducing
cellular apoptosis, triggering excess reactive oxygen species (ROS) production, and
releasing zinc ions due to their inherent nature and specific shape. Therefore,
several new synthetic organometallic method has been developed to prepare ZnO
crystalline nanostructures with controlled size and shape. Zinc oxide
nanostructures' crystal size and shape can be controlled by simply changing the
physical synthesis condition such as microwave irradiation time, reaction
temperature, and TEA concentration at reflux. Physicochemical properties which are
determined by the shape and size of ZnO nanostructures, directly affect their
biological applications. These nanostructures can decompose the cell membrane and
accumulate in the cytoplasm, which leads to apoptosis or cell death. In this study,
we reviewed the various synthesis methods which affect the nano shapes of zinc
particles, and physicochemical properties of zinc nanostructures that determined
the shape of zinc nanomaterials. Also, we mentioned some macromolecules that
controlled their physicochemical properties in a green and biological approaches.
In addition, we present the recent progress of ZnONSs in the biomedical fields,
which will help centralize biomedical fields and assist their future research
development.
AN - WOS:000750370300001
AU - Mousavi, S. M.
AU - Behbudi, G.
AU - Gholami, A.
AU - Hashemi, S. A.
AU - Nejad, Z. M.
AU - Bahrani, S.
AU - Chiang, W. H.
AU - Wei, L. C.
AU - Omidifar, N.
C7 - 4
DA - FEB 2
DO - 10.1186/s40824-022-00252-y
IS - 1
PY - 2022
SN - 1226-4601
2055-7124
ST - Shape-controlled synthesis of zinc nanostructures mediating macromolecules
for biomedical applications
T2 - BIOMATERIALS RESEARCH
TI - Shape-controlled synthesis of zinc nanostructures mediating macromolecules
for biomedical applications
VL - 26
ID - 6626
ER -
TY - JOUR
AB - Levamisole is a veterinary anti-helminthic used to treat several autoimmune
conditions but also commonly utilized as an additive in cocaine distribution.
Toxicity resulting in agranulocytosis and cutaneous necrosis in association with
cocaine use is an infrequently described phenomenon of an emerging problem.
Although levamisole is found extensively in the cocaine supply of the United
States, relatively few cases of necrotic skin lesions associated with intranasal
use have been reported. The skin necrosis secondary to levamisole toxicity is
characterized by variable findings on biopsy, ranging from leukocytoclastic
vasculitis to occlusive vasculopathy. The following case describes a 54-year-old
male who developed fever, agranulocytosis, p-ANCA autoantibodies and extensive skin
necrosis following heavy intranasal cocaine use. Necrosis of greater than 50% of
the patient's total body surface area resulted and was followed by thorough wound
debridement. Copyright © 2011 Journal of Drugs in Dermatology.
AU - Mouzakis, J.
AU - Somboonwit, C.
AU - Lakshmi, S.
AU - Rumbak, M.
AU - Sinnott, J.
AU - Cherpelis, B.
AU - Keshishian, J.
DB - Scopus
IS - 10
KW - Agranulocytosis
Antibodies, Antineutrophil Cytoplasmic
Cocaine
Cocaine-Related Disorders
Drug Contamination
Fever
Humans
Levamisole
Male
Middle Aged
Necrosis
Neutropenia
Skin
United States
bacitracin plus polymyxin B
beta glucan
cardiolipin antibody
cefepime
cocaine
doxycycline
fibrinogen
fluconazole
immunoglobulin M
levamisole
methylprednisolone
neutrophil cytoplasmic antibody
phospholipid
sulfadiazine silver
sulfamethoxazole
trimethoprim
adult
agranulocytosis
arm
article
back
blood culture
body surface
cannabis smoking
case report
cheek
chill
debridement
ear
erythema
erythrocyte sedimentation rate
face
fever
human
leg swelling
leukocyte count
lip
lymphadenopathy
malaise
male
mononuclear cell
neutropenia
neutrophil
neutrophil count
night sweat
nose
partial thromboplastin time
prothrombin time
skin biopsy
skin necrosis
thrombocyte count
trunk
urinalysis
vein thrombosis
M3 - Article
PY - 2011
SP - 1204-1207
ST - Levamisole induced necrosis of the skin and neutropenia following intranasal
cocaine use: A newly recognized syndrome
T2 - Journal of Drugs in Dermatology
TI - Levamisole induced necrosis of the skin and neutropenia following intranasal
cocaine use: A newly recognized syndrome
80054718590&partnerID=40&md5=e7f1c6e7322c9a33cb4970a2f15c04ab
VL - 10
ID - 5743
ER -
TY - JOUR
AB - Pneumonia is a common but serious infectious disease, and is the sixth
leading cause for death. The foreign pathogens such as viruses, fungi, and bacteria
establish an inflammation response after interaction with lung, leading to the
filling of bronchioles and alveoli with fluids. Although the pharmacotherapies have
shown their great effectiveness to combat pathogens, advanced methods are under
developing to treat complicated cases such as virus-infection and lung inflammation
or acute lung injury (ALI). The inflammation modulation nanoparticles (NPs) can
effectively suppress immune cells and inhibit inflammatory molecules in the lung
site, and thereby alleviate pneumonia and ALI. In this review, the pathological
inflammatory microenvironments in pneumonia, which are instructive for the design
of biomaterials therapy, are summarized. The focus is then paid to the
inflammation-modulating NPs that modulate the inflammatory cells, cytokines and
chemokines, and microenvironments of pneumonia for better therapeutic effects. This
article is categorized under: Therapeutic Approaches and Drug Discovery >
Nanomedicine for Respiratory Disease
AN - WOS:000712359400001
AU - Muhammad, W.
AU - Zhai, Z. H.
AU - Wang, S. Q.
AU - Gao, C. Y.
C6 - OCT 2021
C7 - e1763
DA - MAR
DO - 10.1002/wnan.1763
IS - 2
PY - 2022
SN - 1939-5116
1939-0041
ST - Inflammation-modulating nanoparticles for pneumonia therapy
T2 - WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
TI - Inflammation-modulating nanoparticles for pneumonia therapy
VL - 14
ID - 6811
ER -
TY - JOUR
AB - Graphene-based 2D nanomaterials exhibit unique physicochemical, electric, and
optical properties that facilitate applications in a wide range of fields including
material science, electronics, and biotechnology. Recent studies have shown that
graphene oxide (GO) and reduced graphene oxide (rGO) exhibit antimicrobial effects
on bacteria and viruses. While the bactericidal activity of graphene-based
nanomaterials is related to mechanical and oxidative damage to bacterial membranes,
their antiviral activity has been less explored. Currently available experimental
data are limited and suggest mechanical disruption of viral particles prior to
infection. In this study, the antiviral properties of reduced GO-based
nanocomposites decorated with Ag nanoparticles (rGO-Ag) are evidenced against human
immunodeficiency virus-1 pseudovirus used as an enveloped virus model. By combining
biochemical and original single virus imaging approaches, it is shown that rGO-Ag
induces peroxidation of pseudoviral lipid membrane and that consequent alteration
of membrane properties leads to a reduction in cell entry. In addition, rGO-Ag is
found to be efficiently internalized in the host cell leading to the elevated
expression of pro-inflammatory cytokines. Altogether, the presented results shed
new light on the mechanisms of rGO-Ag antiviral properties and confirm the high
potential of graphene derivatives as an antimicrobial material for biomedical
applications.
AN - WOS:000895508500001
AU - Mukherjee, S.
AU - Bytesnikova, Z.
AU - Martin, S.
AU - Svec, P.
AU - Ridoskova, A.
AU - Pekarkova, J.
AU - Seguin, C.
AU - Weickert, J. L.
AU - Messaddeq, N.
AU - Mely, Y.
AU - Richtera, L.
AU - Anton, H.
AU - Adam, V.
C6 - DEC 2022
DA - FEB
DO - 10.1002/admi.202201996
IS - 6
PY - 2023
SN - 2196-7350
ST - Silver Nanoparticle-Decorated Reduced Graphene Oxide Nanomaterials Exert
Membrane Stress and Induce Immune Response to Inhibit the Early Phase of HIV-1
Infection
T2 - ADVANCED MATERIALS INTERFACES
TI - Silver Nanoparticle-Decorated Reduced Graphene Oxide Nanomaterials Exert
Membrane Stress and Induce Immune Response to Inhibit the Early Phase of HIV-1
Infection
VL - 10
ID - 6453
ER -
TY - JOUR
AB - The aim of this study was to synthesize L-cysteine stabilized silver
nanoparticles of 2 nm in size and determine their impact on cell viability. Silver
particles of this size range stabilized with L-cysteine woule be novel, as a
similar study by Mandal et al. on cysteine capped silver nanoparticles achieved
particles of only 4.4±0.3 nm. Currently, methods to synthesize and separate 2 nm
silver particles effectively are limited. Silver nanoparticles are an ideal
candidate for biological applications due to its natural anti-bacterial, anti-
inflammatory, and anti-platelet properties. For the 1st time, L-cysteine capped
silver nanoparticles 2±1 nm in diameter were synthesized and successfully separated
that also showed a non-significant impact on MCDK cell viability. Synthesis of the
silver nanoparticles was achieved via a reduction of AgNO 3 and NaBH 4. Capping was
achieved via a thiolate linkage between the amino acid and the silver particles
upon addition of L-cysteine to the colloid solution. Particles were stable for up
to 24 hours after which aggregates can be detected. Cell viability was determined
via a trypan blue stain for dead cells, and showed comparable levels of cell death
between control and nanoparticle treated cells. This study adds further evidence to
the role that particle size and surface charge play in overall cell viability from
silver nanoparticles. These characteristics can lead to the design of silver
nanoparticles that maintain clinical functionality while minimizing toxic effects.
© 2012 American Scientific Publishers All rights reserved.
AU - Mukherjee, S.
AU - Menegazzo, N.
AU - Booksh, K.
AU - Dhurjati, P.
AU - Smorodin, V.
AU - Nohe, A.
DB - Scopus
DO - 10.1166/asl.2012.2036
KW - Cysteine
Nanoparticles
Silver
Synthesis
Toxicity
M3 - Article
PY - 2012
SP - 26-33
ST - Synthesis of L-cysteine stabilized silver nanoparticles and their effects on
cell viability
T2 - Advanced Science Letters
TI - Synthesis of L-cysteine stabilized silver nanoparticles and their effects on
cell viability
84861500214&doi=10.1166%2fasl.2012.2036&partnerID=40&md5=137c2a464662b7d537d8e4ac21
a70713
VL - 6
ID - 5716
ER -
TY - JOUR
AB - The purpose of this study was to evaluate the biocompatibility, encrustation
and biodegradation properties of silver nitrate and ofloxacine blended
caprolactone-L-lactide copolymer coated self-reinforced poly-L-lactic acid (SR-
PLLA) urospirals in situ in the male rabbit urethra. SR-PLLA urospirals coated with
10% by weight silver nitrate or 5% by weight ofloxacine blended copolymer or pure
copolymer were inserted into the posterior urethra of 18 male rabbits. No
prophylactic antibiotics were given. The animals were sacrificed 1 or 6 months
after insertion. Urethral tissue reactions were histologically scored
semiquantitavely and the appearence of the stents assessed using scanning electron
microscopy. The biodegradation time of SR-PLLA stents was remarkably reduced by the
caprolactone coating. Silver nitrate and ofloxacine blended copolymer coated
urospirals caused less tissue reaction than urospirals with a pure copolymer
coating. Silver nitrate coating effectively prevented biofilm formation and stent
encrustation. Silver nitrate and ofloxacine blended copolymer coated SR-PLLA
urospirals had good biocompatibility properties in rabbit urethra. In particular,
coating with silver nitrate may provide possibilities of preventing bacterial
adhesion to bioabsorbable stents.
AU - Multanen, M.
AU - Tammela, T. L. J.
AU - Laurila, M.
AU - Seppälä, J.
AU - Välimaa, T.
AU - Törmälä, P.
AU - Talja, M.
DB - Scopus
DO - 10.1007/s00240-002-0252-7
IS - 4
KW - Bioabsorbable
Biocompatibility
Encrustation
Urethral stent
Animals
Anti-Infective Agents, Urinary
Biodegradation, Environmental
Biofilms
Crystallization
Male
Ofloxacin
Polyesters
Rabbits
Silver Nitrate
Stents
Urethra
ofloxacin
polylactic acid
silver nitrate
animal experiment
animal model
article
biocompatibility
biodegradability
biodegradation
controlled study
drug coating
drug effect
infection rate
inflammatory cell
nonhuman
priority journal
rabbit
stent
tissue reaction
M3 - Article
PY - 2002
SP - 227-232
ST - Biocompatibility, encrustation and biodegradation of ofloxacine and silver
nitrate coated poly-L-lactic acid stents in rabbit urethra
T2 - Urological Research
TI - Biocompatibility, encrustation and biodegradation of ofloxacine and silver
nitrate coated poly-L-lactic acid stents in rabbit urethra
0036920067&doi=10.1007%2fs00240-002-0252-
7&partnerID=40&md5=bb962613329c8e61640998e1673fbdf4
VL - 30
ID - 5819
ER -
TY - JOUR
AB - Human biodistribution, bioprocessing and possible toxicity of nanoscale
silver receive increasing health assessment. We prospectively studied commercial
10- and 32-ppm nanoscale silver particle solutions in a single-blind, controlled,
cross-over, intent-to-treat, design. Healthy subjects (n = 60) underwent metabolic,
blood counts, urinalysis, sputum induction, and chest and abdomen magnetic
resonance imaging. Silver serum and urine content were determined. No clinically
important changes in metabolic, hematologic, or urinalysis measures were
identified. No morphological changes were detected in the lungs, heart or abdominal
organs. No significant changes were noted in pulmonary reactive oxygen species or
pro-inflammatory cytokine generation. In vivo oral exposure to these commercial
nanoscale silver particle solutions does not prompt clinically important changes in
human metabolic, hematologic, urine, physical findings or imaging morphology.
Further study of increasing time exposure and dosing of silver nanoparticulate
silver, and observation of additional organ systems are warranted to assert human
toxicity thresholds. From the Clinical Editor: In this study, the effects of
commercially available nanoparticles were studied in healthy volunteers, concluding
no detectable toxicity with the utilized comprehensive assays and tests. As the
authors rightfully state, further studies are definitely warranted. Studies like
this are much needed for the more widespread application of nanomedicine. © 2014
Elsevier Inc.
AU - Munger, M. A.
AU - Radwanski, P.
AU - Hadlock, G. C.
AU - Stoddard, G.
AU - Shaaban, A.
AU - Falconer, J.
AU - Grainger, D. W.
AU - Deering-Rice, C. E.
DB - Scopus
DO - 10.1016/j.nano.2013.06.010
IS - 1
KW - Biological activity nanoparticles
Nanotechnology
Nanotoxicology oral ingestion
Safety research
Adult
Aged
Blood Cell Count
Female
Heart
Humans
Lung
Magnetic Resonance Imaging
Male
Metal Nanoparticles
Middle Aged
Radiography, Thoracic
Silver
Sputum
Urinalysis
Bioactivity
Body fluids
Magnetic resonance imaging
Metabolism
Nanoparticles
Toxicity
silver nanoparticle
Health assessments
Healthy volunteers
Morphological changes
Nano particulates
Oral ingestion
abdomen
article
blood cell count
heart
human
in vivo study
intention to treat analysis
lung
nanotoxicology
normal human
organ systems
prospective study
sputum
urinalysis
M3 - Article
PY - 2014
SP - 1-9
ST - In vivo human time-exposure study of orally dosed commercial silver
nanoparticles
TI - In vivo human time-exposure study of orally dosed commercial silver
nanoparticles
84890978504&doi=10.1016%2fj.nano.2013.06.010&partnerID=40&md5=8dc240f33a59229119add
46f7d9feff9
VL - 10
ID - 5656
ER -
TY - JOUR
AB - The bacterial cellulose membrane (CM) is a promising biomaterial due to its
easy applicability and moist environment. Moreover, nanoscale silver compounds
(AgNO3) are synthesized and incorporated into CMs to provide these biomaterials
with antimicrobial activity for wound healing. This study aimed to evaluate the
cell viability of CM incorporated with nanoscale silver compounds, determine the
minimum inhibitory concentration (MIC) for Escherichia coli and Staphylococcus
aureus, and its use on in vivo skin lesions. Wistar rats were divided according to
treatment: untreated, CM (cellulose membrane), and AgCM (CM incorporated with
silver nanoparticles). The euthanasia was performed on the 2nd, 7th, 14th, and 21st
days to assess inflammation (myeloperoxidase-neutrophils, N-acetylglucosaminidase-
macrophage, IL-1β, IL-10), oxidative stress (NO-nitric oxide, DCF-H2O2), oxidative
damage (carbonyl: membrane's damage; sulfhydryl: membrane's integrity),
antioxidants (superoxide dismutase; glutathione), angiogenesis, tissue formation
(collagen, TGF-β1, smooth muscle α-actin, small decorin, and biglycan
proteoglycans). The use of AgCM did not show toxicity, but antibacterial effect in
vitro. Moreover, in vivo, AgCM provided balanced oxidative action, modulated the
inflammatory profile due to the reduction of IL-1β level and increase in IL-10
level, in addition to increased angiogenesis and collagen formation. The results
suggest the use of silver nanoparticles (AgCM) enhanced the CM properties by
providing antibacterial properties, modulation the inflammatory phase, and
consequently promotes the healing of skin lesions, which can be used clinically to
treat injuries. © 2023 Elsevier Inc.
AU - Munhoz, L. L. S.
AU - Alves, M. T. O.
AU - Alves, B. C.
AU - Nascimento, M. G. F. S.
AU - Sábio, R. M.
AU - Manieri, K. F.
AU - Barud, H. S.
AU - Esquisatto, M. A. M.
AU - Aro, A. A.
AU - de Roch Casagrande, L.
AU - Silveira, P. C. L.
AU - Santos, G. M. T.
AU - Andrade, T. A. M.
AU - Caetano, G. F.
DB - Scopus
DO - 10.1016/j.bbrc.2023.02.058
KW - Bacterial cellulose
Biomembrane
Inflammation
Oxidative stress
Tissue formation
Animals
Bacteria
Cellulose
Collagen
Hydrogen Peroxide
Interleukin-10
Metal Nanoparticles
Models, Animal
Rats
Rats, Wistar
Silver
Wound Healing
acetylglucosaminidase
bacterial cellulose membrane
biomaterial
carbonyl derivative
collagen
decorin
dichlorofluorescein
glutathione
hydrogen peroxide
interleukin 10
interleukin 1beta
myeloperoxidase
nitric oxide
proteoglycan
silver nanoparticle
thiol derivative
unclassified drug
antiinfective agent
cellulose
metal nanoparticle
silver
angiogenesis
animal cell
animal experiment
animal model
animal tissue
Article
bacterial viability
collagen synthesis
controlled study
drug safety
Escherichia coli
euthanasia
histogenesis
in vitro study
in vivo study
inflammation
macrophage
male
neutrophil
nonhuman
oxidative stress
rat
skin defect
Wistar rat
wound healing
animal
bacterium
M3 - Article
PY - 2023
SP - 47-54
ST - Bacterial cellulose membrane incorporated with silver nanoparticles for wound
healing in animal model
T2 - Biochemical and Biophysical Research Communications
TI - Bacterial cellulose membrane incorporated with silver nanoparticles for wound
85149468065&doi=10.1016%2fj.bbrc.2023.02.058&partnerID=40&md5=4d742e9403b5b07637f9a
70b1b0995c5
VL - 654
ID - 5046
ER -
TY - JOUR
AB - The bacterial cellulose membrane (CM) is a promising biomaterial due to its
easy applicability and moist environment. Moreover, nanoscale silver compounds
(AgNO3) are synthesized and incorporated into CMs to provide these biomaterials
with antimicrobial activity for wound healing. This study aimed to evaluate the
cell viability of CM incorporated with nanoscale silver compounds, determine the
minimum inhibitory concentration (MIC) for Escherichia coli and Staphylococcus
aureus, and its use on in vivo skin lesions. Wistar rats were divided according to
treatment: untreated, CM (cellulose membrane), and AgCM (CM incorporated with
silver nanoparticles). The euthanasia was performed on the 2nd, 7th, 14th, and 21st
days to assess inflammation (myeloperoxidase-neutrophils, N-acetylglucosaminidase-
macro-phage, IL-10, IL-10), oxidative stress (NO-nitric oxide, DCF-H2O2), oxidative
damage (carbonyl: mem-brane's damage; sulfhydryl: membrane's integrity),
antioxidants (superoxide dismutase; glutathione), angiogenesis, tissue formation
(collagen, TGF-01, smooth muscle a-actin, small decorin, and biglycan
proteoglycans). The use of AgCM did not show toxicity, but antibacterial effect in
vitro. Moreover, in vivo, AgCM provided balanced oxidative action, modulated the
inflammatory profile due to the reduction of IL-10 level and increase in IL-10
level, in addition to increased angiogenesis and collagen formation. The results
suggest the use of silver nanoparticles (AgCM) enhanced the CM properties by
providing anti-bacterial properties, modulation the inflammatory phase, and
consequently promotes the healing of skin lesions, which can be used clinically to
treat injuries.(c) 2023 Elsevier Inc. All rights reserved.
AN - WOS:000953372700001
AU - Munhoz, L. L. S.
AU - Alves, M. T. O.
AU - Alves, B. C.
AU - Nascimento, Mgfs
AU - Sabio, R. M.
AU - Manieri, K. F.
AU - Barud, H. S.
AU - Esquisatto, M. A. M.
AU - Aro, A. A.
AU - Casagrande, L. D.
AU - Silveira, P. C. L.
AU - Santos, G. M. T.
AU - Andrade, T. A. M.
AU - Caetano, G. F.
C6 - MAR 2023
DA - APR 30
DO - 10.1016/j.bbrc.2023.02.058
PY - 2023
SN - 0006-291X
1090-2104
SP - 47-54
ST - Bacterial cellulose membrane incorporated with silver nanoparticles for wound
T2 - BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
TI - Bacterial cellulose membrane incorporated with silver nanoparticles for wound
VL - 654
ID - 5883
ER -
TY - JOUR
AB - A complete cytotoxic profile of exposure to silver (AgNP) nanoparticles
investigating their biological effects on the innate immune response of circulating
white blood cells is required to form a complete understanding of the risk posed.
This was explored by measuring AgNP-stimulated gene expression of the pro-
inflammatory cytokines interleukin-1 (IL-1), interleukin-6 (IL-6) and tumour
necrosis factor-alpha (TNF-α) in THP-1 monocytes. A further study, on human
monocytes extracted from a cohort of blood samples, was carried out to compare with
the AgNP immune response in THP-1 cells along with the detection of pro-IL-1β which
is a key mediator of the inflammasome complex. The aims of the study were to
clearly demonstrate that AgNP can significantly up-regulate pro-inflammatory
cytokine gene expression of IL-1, IL-6 and TNF-α in both THP-1 cells and primary
blood monocytes thus indicating a rapid response to AgNP in circulation.
Furthermore, a role for the inflammasome in AgNP response was indicated by pro-IL-
1β cleavage and release. These results highlight the potential inflammatory effects
of AgNP exposure and the responses evoked should be considered with respect to the
potential harm that exposure may cause. Copyright © 2016 John Wiley & Sons, Ltd.
Copyright © 2016 John Wiley & Sons, Ltd.
AU - Murphy, A.
AU - Casey, A.
AU - Byrne, G.
AU - Chambers, G.
AU - Howe, O.
DB - Scopus
DO - 10.1002/jat.3315
IS - 10
KW - IL-1β release
innate immune response
nanoparticle exposure
inflammasome
interleukin 1
interleukin 6
lipopolysaccharide
silver
silver nanoparticle
Article
blood sampling
cohort analysis
controlled study
cytokine release
cytotoxicity
exposure
gene expression
human
human cell
immune response
inflammation
monocyte
priority journal
protein cleavage
upregulation
M3 - Article
PY - 2016
SP - 1311-1320
ST - Silver nanoparticles induce pro-inflammatory gene expression and inflammasome
activation in human monocytes
TI - Silver nanoparticles induce pro-inflammatory gene expression and inflammasome
activation in human monocytes
84982920999&doi=10.1002%2fjat.3315&partnerID=40&md5=f4bea19d27e5e0919d2beb7ae5bfae3
2
VL - 36
ID - 5523
ER -
TY - JOUR
AB - Nanotechnology is a rapidly growing field with silver nanoparticles (AgNP) in
particular utilized in a wide variety of consumer products. This has presented a
number of concerns relating to exposure and the associated toxicity to humans and
the environment. As inhalation is the most common exposure route, this study
investigates the potential toxicity of AgNP to A549 alveolar epithelial carcinoma
cells and the influence of a major component of lung surfactant
dipalmitoylphosphatidylcholine (DPPC) on toxicity. It was illustrated that exposure
to AgNP generated low levels of oxidative stress and a reduction in cell viability.
While DPPC produced no significant effect on viability studies its presence
resulted in increased reactive oxygen species formation. DPPC also significantly
modified the inflammatory response generated by AgNP exposure. These findings
suggest a possible interaction between AgNP and DPPC causing particles to become
more reactive, thus increasing oxidative insult and inflammatory response within
A549 cells. © 2015 John Wiley & Sons, Ltd.
AU - Murphy, A.
AU - Sheehy, K.
AU - Casey, A.
AU - Chambers, G.
DB - Scopus
DO - 10.1002/jat.3148
IS - 10
KW - Acute response
DPPC
Modify
1,2-Dipalmitoylphosphatidylcholine
Adenocarcinoma, Bronchiolo-Alveolar
Cell Line, Tumor
Cell Survival
Coloring Agents
Humans
Inflammation
Lung Neoplasms
Metal Nanoparticles
Oxazines
Silver
Tetrazolium Salts
Thiazoles
Tumor Stem Cell Assay
Xanthenes
dipalmitoylphosphatidylcholine
interleukin 8
silver nanoparticle
coloring agent
metal nanoparticle
oxazine derivative
resazurin
silver
tetrazolium
thiazole derivative
thiazolyl blue
xanthene derivative
A549 cell line
Article
carcinoma cell
cell culture
cell death
cell survival
cell viability
colony formation
controlled study
cytokine release
cytotoxicity
dynamic light scattering
human
human cell
hydrodynamics
IC50
inflammation
light scattering
lung alveolus cell carcinoma
MTT assay
oxidative stress
particle size
priority journal
toxicity testing
zeta potential
chemically induced
drug effects
lung tumor
metabolism
pathology
tumor cell line
tumor stem cell assay
M3 - Article
PY - 2015
SP - 1141-1149
ST - The surfactant dipalmitoylphophatidylcholine modifies acute responses in
alveolar carcinoma cells in response to low-dose silver nanoparticle exposure
TI - The surfactant dipalmitoylphophatidylcholine modifies acute responses in
alveolar carcinoma cells in response to low-dose silver nanoparticle exposure
84939261075&doi=10.1002%2fjat.3148&partnerID=40&md5=6d376707628ebe08167b94eef8fb467
3
VL - 35
ID - 5621
ER -
TY - JOUR
AB - The use of nanoparticles (NPs) for developing new therapeutic strategies in a
variety of diseases is gaining increasing attention. However, NPs could possess
undesired effects, including pro-inflammatory activities. Despite the fact that
several studies reported that NPs may induce or exacerbate eosinophilic
inflammation in vivo in rodents, the information regarding the direct interaction
between NPs and human eosinophils is lacking. In the present study, we test the
possibility that NPs could alter the capacity of human eosinophils to adhere onto a
cellular substratum. Using a panel of NPs, we found that several were able to
increase the adhesion of human eosinophil onto endothelial EA.hy926 cells. Among
them, TiO2 NPs were the most potent and we therefore pursue this study with these
NPs. TiO2 NPs were found to increase the adhesion of eosinophils in a concentration
dependent fashion. TiO2 NPs did not alter the cell surface expression of a panel of
cellular adhesion molecules, but CD29. Indeed, a weak to moderate, but significant,
decrease of CD29 was observed after 30 min but returned to normal levels after 90
min. TiO2 NPs were found to activate Akt, one important target of phosphoinositide
3-kinase (PI3K). However, despite the fact that cells were fully responsive to the
cytokine GM-CSF activating both Ala and Erk-1/2, TiO2 NPs did not activate Erk-1/2.
Using a pharmacological approach with the PI3K/Akt inhibitor, wortmannin, the
ability of TiO2 NPs to activate Akt was drastically inhibited and, further, their
capacity to increase adhesion of eosinophils was reversed. This study provides
insights into the effects of NPs on the biology of human eosinophils indicating
that as other agents, NPs, namely TiO2 NPs, can induce intracellular events
associated with a cellular function, adhesion.
AN - WOS:000419263000002
AU - Murphy-Marion, M.
AU - Girard, D.
DA - FEB
DO - 10.1016/j.imbio.2017.10.030
IS - 2
PY - 2018
SN - 0171-2985
1878-3279
SP - 162-170
ST - Titanium dioxide nanoparticles induce human eosinophil adhesion onto
endothelial EA.hy926 cells via activation of phosphoinositide 3-kinase/Akt cell
signalling pathway (Withdrawn Publication)
T2 - IMMUNOBIOLOGY
TI - Titanium dioxide nanoparticles induce human eosinophil adhesion onto
endothelial EA.hy926 cells via activation of phosphoinositide 3-kinase/Akt cell
signalling pathway (Withdrawn Publication)
VL - 223
ID - 6570
ER -
TY - JOUR
AB - Bacterial biofilm formation and antibiotic resistance are the main factors of
surgical wound complications. Traditional treatments in some cases cannot provide
complete bacterial eradication and new therapeutic approaches should be developed
to overcome antibiotic resistance. Silver nanoparticles (AgNPs) can be the first
choice for bacteria treatment but their clinical application is limited due to
toxic effects. Combination of AgNPs with the low-frequency ultrasound (US)
treatment expected to decrease toxicity and leads to the facilitation of wound
healing. In current research we investigated the antibacterial activity of AgNPs
per se and in combination with low-frequency US, assessed the cytotoxicity of AgNPs
on human dermal fibroblasts and finally, wound healing was evaluated in purulent
wound model (96 white laboratory rats) applying AgNPs and US as a treatment
strategy. Our results demonstrate no toxic effect of AgNPs in minimum inhibitory
concentrations and show increasing their antibacterial effectiveness after US
application. The combination of low-frequency US and AgNPs provides reduction of
the inflammatory reaction, microorganism elimination and leads to facilitation of
new tissue formation with complete epithelization. All effects were significant
over the Chlorhexidine treatment, monotherapy with AgNPs or US. Advanced
effectiveness of complex therapy opens new perspectives for clinical application of
AgNPs solution accompanied by US. © 2020, Korean Society of Medical and Biological
Engineering.
AU - Myronov, P.
AU - Bugaiov, V.
AU - Holubnycha, V.
AU - Sikora, V.
AU - Deineka, V.
AU - Lyndin, M.
AU - Opanasyuk, A.
AU - Romaniuk, A.
AU - Pogorielov, M.
DB - Scopus
DO - 10.1007/s13534-020-00174-5
IS - 4
Cytotoxicity
Purulent wounds
Ultrasound
Antibiotics
Bacteria
Cell culture
Industrial poisons
Metal nanoparticles
Tissue regeneration
Ultrasonics
chlorhexidine
ethylene glycol
fibrin
polyol
silver nanoparticle
Clinical application
Human dermal fibroblasts
Low-frequency ultrasound
angiogenesis
animal experiment
animal model
animal tissue
Article
bacterial clearance
bacterial growth
biofilm
cell infiltration
cell viability
colony forming unit
controlled study
cytotoxicity
drug analysis
drug efficacy
drug synthesis
echography
edema
epithelization
Escherichia coli
fibroblast
granulation tissue
growth inhibition
histiocyte
histopathology
inflammation
low frequency ultrasound
microbiological examination
microscopy
model
monotherapy
morphometry
necrosis
nonhuman
particle size
pathogen clearance
proliferation index
purulent wound
rat
squamous epithelium
subcutaneous fat
swelling
thrombosis
tissue regeneration
venous congestion
wound
wound care
wound healing
Silver compounds
M3 - Article
PY - 2020
SP - 621-631
ST - Low-frequency ultrasound increase effectiveness of silver nanoparticles in a
purulent wound model
T2 - Biomedical Engineering Letters
TI - Low-frequency ultrasound increase effectiveness of silver nanoparticles in a
purulent wound model
85092729985&doi=10.1007%2fs13534-020-00174-
5&partnerID=40&md5=3e8222b85a67e0c4d00f0b565453cf40
VL - 10
ID - 5304
ER -
TY - JOUR
AB - The development and use of nanoparticles have alerted toxicologists and
regulators to issues of safety testing. By analogy with ambient air particles, it
can be expected that small doses are associated with a small increase in risk of
cardiovascular diseases, possibly through oxidative stress and inflammatory
pathways. We have assessed the effect of exposure to particulate matter on
progression of atherosclerosis and vasomotor function in humans, animals, and ex
vivo experimental systems. The type of particles that have been tested in these
systems encompass TiO2, carbon black, fullerene C60, single-walled carbon
nanotubes, ambient air particles, and diesel exhaust particles. Exposure to ambient
air particles is associated with accelerated progression of atherosclerosis and
vasomotor dysfunction in both healthy and susceptible animal models and humans at
risk of developing cardiovascular diseases. The vasomotor dysfunction includes
increased vasoconstriction as well as reduced endothelium-dependent vasodilatation;
endothelium-independent vasodilatation is often unaffected indicating mainly
endothelial dysfunction. Pulmonary exposure to TiO2, carbon black, and engineered
nanoparticles generate vasomotor dysfunction; the effect size is similar to that
generated by combustion-derived particles, although the effect could depend on the
exposure period and the administered dose, route, and mode. The relative risk
associated with exposure to nanoparticles may be small compared to some traditional
risk factors for cardiovascular diseases, but superimposed on these and possible
exposure to large parts of the population it is a significant public health
concern. Overall, assessment of vasomotor dysfunction and progression of
atherosclerosis are promising tools for understanding the effects of particulate
matter. © 2011 Informa Healthcare USA, Inc.
AU - Møller, P.
AU - Mikkelsen, L.
AU - Vesterdal, L. K.
AU - Folkmann, J. K.
AU - Forchhammer, L.
AU - Roursgaard, M.
AU - Danielsen, P. H.
AU - Loft, S.
DB - Scopus
DO - 10.3109/10408444.2010.533152
IS - 4
KW - Air pollution
atherosclerosis
endothelial dysfunction
nanoparticles
Air Pollutants
Animals
Atherosclerosis
Blood Vessels
Disease Progression
Humans
Nanoparticles
Particulate Matter
Vasoconstriction
Vasodilation
Vasomotor System
Animalia
1,1 diethyl 2 hydroxy 2 nitrosohydrazine
15 hydroxy 11alpha,9alpha epoxymethanoprosta 5,13 dienoic acid
3,3 bis(2 aminoethyl) 1 hydroxy 2 oxotriazene
adrenergic receptor blocking agent
apolipoprotein E
black carbon
calcimycin
cyclo(dextro tryptophyl dextro aspartylprolyl dextro valylleucyl)
fullerene
glyceryl trinitrate
hydrazine derivative
n (2,6 dimethylpiperidinocarbonyl) 4 methylleucyl dextro (1
methoxycarbonyltryptophanyl) dextro norleucine
nanoparticle
nitroprusside sodium
ozone
phenylephrine
silver nanoparticle
titanium dioxide
unclassified drug
vasodilator agent
verapamil
air pollution
ambient air
blood flow
blood vessel
cell proliferation
combustion
disease course
ecotoxicity
endothelium
ex vivo study
forearm blood flow
health hazard
human
inhalation
intracoronary infusion
lung
lung toxicity
nonhuman
particle size
particulate matter
public health
review
risk assessment
risk factor
shear stress
vasoconstriction
vasodilatation
vasomotor disorder
M3 - Review
PY - 2011
SP - 339-368
ST - Hazard identification of particulate matter on vasomotor dysfunction and
progression of atherosclerosis
T2 - Critical Reviews in Toxicology
TI - Hazard identification of particulate matter on vasomotor dysfunction and
progression of atherosclerosis
79953134999&doi=10.3109%2f10408444.2010.533152&partnerID=40&md5=73077ada14eb5fc6a11
682bda22f124f
VL - 41
ID - 5732
ER -
TY - JOUR
AB - Introduction: The aim of this study was to assess the biocompatibility of
positively charged imidazolium-based ionic liquid-protected nanosilver solution
(AgNPs) root canal irrigant. Methods and Materials: Eighteen male 4- to 5-month old
Sprague-Dawley rats, weighing 200- 300 gr were selected and randomly divided into 5
groups: Normal saline 0.9% (group 1), 5.25% NaOCl (group 2), 2.5% NaOCl (group 3),
2.0% chlorhexidine solution (group 4) and AgNPs at 5.7×10-8M/L (group 5) were
randomly injected in 5 sites of dorsal skin of each rat. Tissue inflammatory
reaction were evaluated histopathologically after 2 h, 48 h and 14 days.
Statistical analysis was done with SPSS version 21 and the Kruskal-Wallis H and
Dunn tests were used to find statistically significant differences. The level of
significance was set at 0.05. Result: All solutions irritated the highest tissue
response after 48 h. Group 1 showed lower inflammatory response compared to groups
2 and 4 (P<0.05). Group 2 displayed higher inflammatory response in comparison with
group 5 (P<0.05). Tissue reaction to group 5 was not more severe than the reaction
to group 3 or 4. It also would irritate less inflammatory response compared to
group 2 (P<0.05). Conclusion: Comparing with NaOCl and CHX, it is possible to label
AgNPs as a tissue compatible agent. © 2018, Iranian Association of Endodontics. All
rights reserved.
AU - Nabavizadeh, M.
AU - Ghahramani, Y.
AU - Abbaszadegan, A.
AU - Jamshidzadeh, A.
AU - Jenabi, P.
AU - Makarempour, A.
DB - Scopus
DO - 10.22037/iej.v13i3.17386
IS - 3
Root canal irrigant
Silver nanoparticle
M3 - Article
PY - 2018
SP - 293-298
ST - In vivo biocompatibility of an ionic liquid-protected silver nanoparticle
solution as root canal irrigant
T2 - Iranian Endodontic Journal
TI - In vivo biocompatibility of an ionic liquid-protected silver nanoparticle
solution as root canal irrigant
85049889550&doi=10.22037%2fiej.v13i3.17386&partnerID=40&md5=6057145295b484196435ab4
3476b8e06
VL - 13
ID - 5552
ER -
TY - JOUR
AB - Silver is known to be a potent bactericidal agent. It has a proinflammatory
effect by inducing inflammatory cytokines and reactive oxygen species. Silver can
also have a direct cytotoxic effect on immune cells and can cause endothelial cell
injury.(1) At low levels, silver is thought to be nontoxic. It only deposits in the
skin and causes a silver-blue discoloration called argyria. Lately, there has been
an increase in the use of silver nanoparticles as antimicrobial agents or via
alternative medicine, which reignited interest in further studying silver toxicity.
In the CNS, silver can disrupt the blood-brain barrier and be toxic to neurons and
astrocytes.(1) There have been case reports of silver toxicity associated with
seizures, cortical basal degeneration, and psychosis.(2-4) On the other hand, the
effect of silver on the peripheral nervous system remains poorly explored. In this
report, we present a case of a peripheral neuropathy associated with argyria with
detailed clinical, laboratory, and histopathologic findings.
AN - WOS:000463252900005
AU - Naddaf, E.
AU - Dyck, P. J.
AU - Jannetto, P. J.
AU - Murray, D. L.
AU - Dyck, P. J. B.
DA - MAR 5
DO - 10.1212/WNL.0000000000007048
IS - 10
PY - 2019
SN - 0028-3878
1526-632X
SP - 481-483
ST - Peripheral neuropathy associated with silver toxicity
T2 - NEUROLOGY
TI - Peripheral neuropathy associated with silver toxicity
VL - 92
ID - 6133
ER -
TY - JOUR
AB - Solutions containing Ag0 nanoclusters, Ag+1, and higher oxidation state
silver, generated from nanocrystalline silver dressings, were anti-inflammatory
against porcine skin inflammation. The dressings have clinically-demonstrated
broad-spectrum antimicrobial activity, suggesting application of nanosilver
solutions in treating pulmonary infection. Nanosilver solutions were tested for
antimicrobial efficacy; against HSV-1 and SARS-CoV-2; and nebulized in rats with
acute pneumonia. Patients with pneumonia (ventilated), fungal sinusitis, burns plus
COVID-19, and two non-hospitalized patients with COVID-19 received nebulized
nanosilver solution. Nanosilver solutions demonstrated pH-dependent antimicrobial
efficacy; reduced infection and inflammation without evidence of lung toxicity in
the rat model; and inactivated HSV-1 and SARS-CoV-2. Pneumonia patients had rapidly
reduced pulmonary symptoms, recovering pre-illness respiratory function. Fungal
sinusitis-related inflammation decreased immediately with infection clearance
within 21 days. Non-hospitalized patients with COVID-19 experienced rapid symptom
remission. Nanosilver solutions, due to anti-inflammatory, antiviral, and
antimicrobial activity, may be effective for treating respiratory inflammation and
infections caused by viruses and/or microbes. © 2023
AU - Nadworny, P. L.
AU - Hickerson, W. L.
AU - Holley-Harrison, H. D.
AU - Bloom, D. C.
AU - Grams, T. R.
AU - Edwards, T. G.
AU - Schultz, G. S.
AU - Burrell, R. E.
C7 - 102654
DB - Scopus
DO - 10.1016/j.nano.2023.102654
Antimicrobial
Antiviral
SARS-CoV-2
Animals
COVID-19
Inflammation
Pneumonia
Rats
Silver
Sinusitis
Swine
Fungi
Nanocrystals
Pathology
antiinfective agent
antivirus agent
colloidal silver
gelatinase A
gelatinase B
halothane
silver nanoparticle
silver nitrate
steroid
tobramycin
silver
Ag +
Anti-inflammatories
Antivirals
High oxidation state
Nano silver
Porcine skin
Silver dressing
adolescent
adult
allergic contact dermatitis
animal experiment
animal model
animal tissue
apoptosis
Article
atomic absorption
bacterial pneumonia
bronchoscopy
burn
burn patient
cell infiltration
clinical evaluation
contact dermatitis
controlled study
coronavirus disease 2019
cream
dissolution
edema
epithelium cell
erythema
fungal sinusitis
guinea pig model
Human alphaherpesvirus 1
in vitro study
inflammatory cell
inoculation
interstitial pneumonia
multidrug resistant pneumonia
nebulization
neutrophil
nonhuman
off label drug use
oxygen consumption
pathogen clearance
pig
plaque forming unit
pneumonia
rat
remission
thorax radiography
total body surface area
upper respiratory tract infection
virus inactivation
virus strain
animal
complication
inflammation
sinusitis
Coronavirus
M3 - Article
PY - 2023
ST - Treatment of infection and inflammation associated with COVID-19, multi-drug
resistant pneumonia and fungal sinusitis by nebulizing a nanosilver solution
TI - Treatment of infection and inflammation associated with COVID-19, multi-drug
resistant pneumonia and fungal sinusitis by nebulizing a nanosilver solution
85146655457&doi=10.1016%2fj.nano.2023.102654&partnerID=40&md5=1ace74f4a00f77748138d
1631b746aa3
VL - 48
ID - 4960
ER -
TY - JOUR
AB - This study examined the mechanism of nanocrystalline silver antiinflammatory
activity, and tested nanocrystalline silver for systemic antiinflammatory effects.
Secondary ion mass spectroscopy of skin treated directly with nanocrystalline
silver for 24 hours showed that at skin surfaces there were significant deposits at
weights corresponding to Ag, AgO, AgCl, AgNO3, Ag2O, and silver clusters Ag2-6, but
silver penetration was minimal. To test for translocation of the effect, a porcine
contact dermatitis model in which wounds were induced on one side of the back and
then treated with nanocrystalline silver on the opposite side of the back was used.
Visual and histological data showed improvement relative to animals treated with
saline only. Significantly increased induction of apoptosis in the inflammatory
cells present in the dermis was observed with remote nanocrystalline silver
treatments. In addition, immunohistochemical analysis showed decreased levels of
proinflammatory cytokines tumor necrosis factor-alpha and interleukin-8, and
increased levels of antiinflammatory cytokine interleukin-4, epidermal growth
factor, keratinocyte growth factor, and keratinocyte growth factor-2. Thus, the
antiinflammatory effects of nanocrystalline silver appear to be induced by
interactions with cells in the top layers of the skin, which then release
biological signals resulting in widespread antiinflammatory activity.
AN - WOS:000275760600015
AU - Landry, B. K.
AU - Wang, J. F.
AU - Tredget, E. E.
AU - Burrell, R. E.
DA - MAR-APR
DO - 10.1111/j.1524-475X.2010.00579.x
IS - 2
PY - 2010
SN - 1067-1927
1524-475X
SP - 254-265
ST - Does nanocrystalline silver have a transferable effect?
T2 - WOUND REPAIR AND REGENERATION
TI - Does nanocrystalline silver have a transferable effect?
VL - 18
ID - 6372
ER -
TY - JOUR
AB - This study examined the mechanism of nanocrystalline silver antiinflammatory
activity, and tested nanocrystalline silver for systemic antiinflammatory effects.
Secondary ion mass spectroscopy of skin treated directly with nanocrystalline
silver for 24 hours showed that at skin surfaces there were significant deposits at
weights corresponding to Ag, AgO, AgCl, AgNO3, Ag2O, and silver clusters Ag2-6, but
silver penetration was minimal. To test for translocation of the effect, a porcine
contact dermatitis model in which wounds were induced on one side of the back and
then treated with nanocrystalline silver on the opposite side of the back was used.
Visual and histological data showed improvement relative to animals treated with
saline only. Significantly increased induction of apoptosis in the inflammatory
cells present in the dermis was observed with remote nanocrystalline silver
treatments. In addition, immunohistochemical analysis showed decreased levels of
proinflammatory cytokines tumor necrosis factor-α and interleukin-8, and increased
levels of antiinflammatory cytokine interleukin-4, epidermal growth factor,
keratinocyte growth factor, and keratinocyte growth factor-2. Thus, the
antiinflammatory effects of nanocrystalline silver appear to be induced by
interactions with cells in the top layers of the skin, which then release
biological signals resulting in widespread antiinflammatory activity. © 2010 by the
Wound Healing Society.
AU - Landry, B. K.
AU - Wang, J.
AU - Tredget, E. E.
AU - Burrell, R. E.
DB - Scopus
DO - 10.1111/j.1524-475X.2010.00579.x
IS - 2
KW - Animals
Apoptosis
Cytokines
Fibroblast Growth Factor 10
Fibroblast Growth Factor 7
Mass Spectrometry
Models, Animal
Nanoparticles
Silver
Swine
Wound Healing
interleukin 4
interleukin 8
keratinocyte growth factor
nanocrystal
silver
animal experiment
animal model
animal tissue
apoptosis
article
contact dermatitis
controlled study
mass spectrometry
nonhuman
priority journal
protein blood level
wound dressing
M3 - Article
PY - 2010
SP - 254-265
ST - Does nanocrystalline silver have a transferable effect?
T2 - Wound Repair and Regeneration
TI - Does nanocrystalline silver have a transferable effect?
77949773360&doi=10.1111%2fj.1524-
475X.2010.00579.x&partnerID=40&md5=05823c8cf9fe01a6609de78ed4791db3
VL - 18
ID - 5674
ER -
TY - JOUR
AB - The anti-inflammatory activity of nanocrystalline silver was examined using a
porcine model of contact dermatitis. Inflammation was induced with
dinitrochlorobenzene and then treated daily with nanocrystalline silver dressings,
0.5% silver nitrate, or saline. Erythema, edema, and histological data showed that
nanocrystalline silver-treated pigs had near-normal skin after 72 hours, while
other treatment groups remained inflamed. The decreased inflammation in the
nanocrystalline silver-treated group was associated with increased inflammatory
cell apoptosis, a decreased expression of proinflammatory cytokines, and decreased
gelatinase activity. Silver nitrate treatments induced apoptosis in all cell types,
including keratinocytes, resulting in delayed wound healing. These results
demonstrate that nanocrystalline silver had a direct anti-inflammatory effect in
the porcine contact dermatitis model that improved the overall outcome of the
healing process. These data offer support that a species of silver (e. g., Ag-0)
that is uniquely associated with nanocrystalline silver may be responsible for the
anti-inflammatory activity and improvement in healing. (C) 2008 Elsevier Inc. All
rights reserved.
AN - WOS:000262668500008
AU - Wang, J. F.
AU - Tredget, E. E.
AU - Burrell, R. E.
DA - SEP
DO - 10.1016/j.nano.2008.04.006
IS - 3
PY - 2008
SN - 1549-9634
1549-9642
SP - 241-251
ST - Anti-inflammatory activity of nanocrystalline silver in a porcine contact
dermatitis model
TI - Anti-inflammatory activity of nanocrystalline silver in a porcine contact
dermatitis model
VL - 4
ID - 5864
ER -
TY - JOUR
AB - The anti-inflammatory activity of nanocrystalline silver was examined using a
porcine model of contact dermatitis. Inflammation was induced with
dinitrochlorobenzene and then treated daily with nanocrystalline silver dressings,
0.5% silver nitrate, or saline. Erythema, edema, and histological data showed that
nanocrystalline silver-treated pigs had near-normal skin after 72 hours, while
other treatment groups remained inflamed. The decreased inflammation in the
nanocrystalline silver-treated group was associated with increased inflammatory
cell apoptosis, a decreased expression of proinflammatory cytokines, and decreased
gelatinase activity. Silver nitrate treatments induced apoptosis in all cell types,
including keratinocytes, resulting in delayed wound healing. These results
demonstrate that nanocrystalline silver had a direct anti-inflammatory effect in
the porcine contact dermatitis model that improved the overall outcome of the
healing process. These data offer support that a species of silver (e.g., Ag0) that
is uniquely associated with nanocrystalline silver may be responsible for the anti-
inflammatory activity and improvement in healing. © 2008 Elsevier Inc. All rights
reserved.
AU - Wang, J.
AU - Tredget, E. E.
AU - Burrell, R. E.
DB - Scopus
DO - 10.1016/j.nano.2008.04.006
IS - 3
Contact dermatitis
Nanocrystalline
Porcine
Silver
Animals
Apoptosis
Bandages
Cellulose
Crystallization
Dermatitis, Contact
Keratinocytes
Nanostructures
Polyesters
Polyethylene
Silver Nitrate
Sus scrofa
Suidae
Sus
Cell death
Health
Nanocrystalline alloys
Nanocrystalline materials
Nitrates
Pathology
Silver alloys
cytokine
gelatinase
silver
silver nitrate
sodium chloride
unclassified drug
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
cell type
contact dermatitis
controlled study
edema
enzyme activity
erythema
healing
histology
inflammation
inflammatory cell
keratinocyte
nonhuman
protein expression
treatment outcome
wound dressing
wound healing
Cell apoptosis
Cell types
Dinitrochlorobenzene
Gelatinase
Healing processes
Histological data
Induced apoptosis
Normal skin
Porcine models
Silver nitrates
Wound healing
M3 - Article
PY - 2008
SP - 241-251
ST - Anti-inflammatory activity of nanocrystalline silver in a porcine contact
dermatitis model
TI - Anti-inflammatory activity of nanocrystalline silver in a porcine contact
dermatitis model
49649122769&doi=10.1016%2fj.nano.2008.04.006&partnerID=40&md5=443a32aca8f34ff9f6d85
7f2ceac87b0
VL - 4
ID - 5773
ER -
TY - JOUR
AB - Background. Nanocrystalline silver dressings have anti-inflammatory activity,
unlike solutions containing Ag+ only, which may be due to dissolution of multiple
silver species. These dressings can only be used to treat surfaces. Thus, silver-
containing solutions with nanocrystalline silver properties could be valuable for
treating hard-to-dress surfaces and inflammatory conditions of the lungs and
bowels. This study tested nanocrystalline silver-derived solutions for anti-
inflammatory activity. Methods. Inflammation was induced on porcine backs using
dinitrochlorobenzene. Negative and positive controls were treated with distilled
water. Experimental groups were treated with solutions generated by dissolving
nanocrystalline silver in distilled water adjusted to starting pHs of 4 (using
CO2), 5.6 (as is), 7, and 9 (using Ca(OH)2). Solution samples were analyzed for
total silver. Daily imaging, biopsying, erythema and oedema scoring, and treatments
were performed for three days. Biopsies were processed for histology,
immunohistochemistry (for IL-4, IL-8, IL-10, TNF-, EGF, KGF, KGF-2, and apoptotic
cells), and zymography (MMP-2 and -9). One-way ANOVAs with Tukey-Kramer post tests
were used for statistical analyses. Results. Animals treated with pH 7 and 9
solutions showed clear visual improvements. pH 9 solutions resulted in the most
significant reductions in erythema and oedema scores. pH 4 and 7 solutions also
reduced oedema scores. Histologically, all treatment groups demonstrated enhanced
re-epithelialisation, with decreased inflammation. At 24 h, pMMP-2 expression was
significantly lowered with pH 5.6 and 9 treatments, as was aMMP-2 expression with
pH 9 treatments. In general, treatment with silver-containing solutions resulted in
decreased TNF- and IL-8 expression, with increased IL-4, EGF, KGF, and KGF-2
expression. At 24 h, apoptotic cells were detected mostly in the dermis with pH 4
and 9 treatments, nowhere with pH 5.6, and in both the epidermis and dermis with pH
7. Solution anti-inflammatory activity did not correlate with total silver content,
as pH 4 solutions contained significantly more silver than all others. Conclusions.
Nanocrystalline silver-derived solutions appear to have anti-inflammatory/pro-
healing activity, particularly with a starting pH of 9. Solutions generated
differently may have varying concentrations of different silver species, only some
of which are anti-inflammatory. Nanocrystalline silver-derived solutions show
promise for a variety of anti-inflammatory treatment applications. © 2010 Nadworny
et al; licensee BioMed Central Ltd.
AU - Wang, J.
AU - Tredget, E. E.
AU - Burrell, R. E.
C7 - 13
DB - Scopus
DO - 10.1186/1476-9255-7-13
KW - Animalia
Sus
Acticoat
dermatological agent
gelatinase A
gelatinase B
interleukin 10
interleukin 4
interleukin 8
nanocrystal
silver derivative
unclassified drug
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
contact dermatitis
controlled study
dermis
edema
epidermis
erythema
histopathology
imaging system
nonhuman
pH
protein expression
skin biopsy
swine
wound dressing
zymography
M3 - Article
PY - 2010
ST - Anti-inflammatory activity of nanocrystalline silver-derived solutions in
porcine contact dermatitis
T2 - Journal of Inflammation
TI - Anti-inflammatory activity of nanocrystalline silver-derived solutions in
porcine contact dermatitis
77649305432&doi=10.1186%2f1476-9255-7-
13&partnerID=40&md5=f13610b12b7591b0899f56740923dab6
VL - 7
ID - 5726
ER -
TY - JOUR
AB - In the present study, for the first time, biomimetization of hydroxyapatite
(HA) with Azadirachta indica (AI) was proposed and established its antioxidant,
antibacterial, and anti-inflammatory potential on lipopolysaccharide (LPS). The
ethanolic extract of AI was found rich with phenolics and flavonoids, and
determined their concentration as 8.98 +/- 1.41 mg gallic acid equivalents/g and
5.46 +/- 0.84 mg catechin equivalents/g, respectively. The HA was prepared by sol-
gel method from calcium nitrate tetrahydrate and orthophosphoric acid, and
successfully biomimetization was performed with ethanolic extract of AI. The FTIR
analysis settled that as-synthesized HA-AI composite was comprised of both HA and
AI. The XRD pattern and Zeta potential revealed that the HA-AI composite was
crystalline and negative in charge (-24.0 mV). The average-size distribution,
shape, and size of the HA-AI composite was determined as 238.90 d.nm, spherical,
and 117.90 nm from size distribution, SEM, and HR-TEM analysis, respectively. The
SEM-EDX concluded that the HA-AI composite was comprised of elements of HA as well
as AI. The HA-AI composite presented potential antioxidant activity and its EC50
values (dose required to inhibit about half of the radicals) for ABTS and DPPH
assays were determined as 115.72 +/- 2.33 and 128.51 +/- 1.04 mu g/ml,
respectively. The HA-AI composite showed potent antibacterial activity, and minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) towards
S. aureus (ATCC 700699) and E. coli (ATCC 10536) were correspondingly determined as
266.7 +/- 28.87 and 600.0 +/- 50.0 mu g/ml, and 400.0 +/- 86.6 and 816.7 +/- 76.38
mu g/ml. Most importantly, HA-AI composite presented the potential anti-
inflammatory response toward lipopolysaccharide (LPS) in RAW 264.7 cells. The dose
of 250 mu g/ml of HA-AI composite has shown optimum protection against LPS-induced
stress (1 mu g/ml) by scavenging oxidants and regulating mitochondrial membrane
potential (MMP), inflammatory and apoptotic factors. Thus, this study concluded
that the impartation of potential biofunctional features to HA from plant sources
through biomimetic approach is much beneficial and could find potential application
in dentistry and orthopedic.
AN - WOS:000479132600001
AU - Nagaraj, A.
AU - Samiappan, S.
C7 - 1757
DA - AUG 7
DO - 10.3389/fmicb.2019.01757
PY - 2019
SN - 1664-302X
ST - Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to
Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-
inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells
T2 - FRONTIERS IN MICROBIOLOGY
TI - Presentation of Antibacterial and Therapeutic Anti-inflammatory Potentials to
Hydroxyapatite via Biomimetic With Azadirachta indica: An in vitro Anti-
inflammatory Assessment in Contradiction of LPS-Induced Stress in RAW 264.7 Cells
VL - 10
ID - 6209
ER -
TY - JOUR
AB - Although nitinol is widely used in percutaneous cardiovascular interventions,
a causal relationship between nickel released from implanted cardiovascular devices
and adverse systemic or local biological responses has not been established. The
objective of this study was to investigate the relationship between nitinol surface
processing, in-vivo nickel release, and biocompatibility. Nitinol stents
manufactured using select surface treatments were implanted into the iliac arteries
of minipigs for 6 months. Clinical chemistry profile, complete blood count, serum
and urine nickel analyses were performed periodically during the implantation
period. After explant, stented arteries were either digested and analyzed for local
nickel concentration or fixed and sectioned for histopathological analysis of
stenosis and inflammation within the artery. The results indicated that markers for
liver and kidney function were not different than baseline values throughout 180
days of implantation regardless of surface finish. In addition, white blood cell,
red blood cell, and platelet counts were similar to baseline values for all surface
finishes. Systemic nickel concentrations in serum and urine were not significantly
different between processing groups and comparable to baseline values during 180
days of implantation. However, stents with non optimized surface finishing had
significantly greater nickel levels in the surrounding artery compared to polished
stents. These stents had increased stenosis with potential for local inflammation
compared to polished stents. These findings demonstrate that proper polishing of
nitinol surfaces can reduce in vivo nickel release locally, which may aid in
minimizing adverse inflammatory reactions and restenosis. Statement of significance
Nitinol is a commonly used material in cardiovascular medical devices. However,
relationships between nitinol surface finishing, in-vivo metal ion release, and
adverse biological responses have yet to be established. We addressed this
knowledge gap by implanting single and overlapped nitinol stents with different
surface finishes to assess systemic impact on minipigs (i.e. serum and urine nickel
levels, liver and kidney function, immune and blood count) over the 6 month
implantation period. In addition, nickel levels and histopathology in stented
arteries were analyzed on explant to determine relationships between surface
processing and local adverse tissue reactions. The findings presented here
highlight the importance of surface processing on in-vivo nickel release and
subsequent impact on local biological response for nitinol implants. Published by
Elsevier Ltd on behalf of Acta Materialia Inc.
AN - WOS:000432766900036
AU - Nagaraja, S.
AU - Sullivan, S. J. L.
AU - Stafford, P. R.
AU - Lucas, A. D.
AU - Malkin, E.
DA - MAY
DO - 10.1016/j.actbio.2018.03.036
PY - 2018
SN - 1742-7061
1878-7568
SP - 424-433
ST - Impact of nitinol stent surface processing on in-vivo nickel release and
biological response
TI - Impact of nitinol stent surface processing on in-vivo nickel release and
biological response
VL - 72
ID - 6765
ER -
TY - JOUR
AB - The current study investigates the hepatotoxic effects of two acute doses of
silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on African catfish (Clarias
garepinus) using biochemical, histopathological, and histochemical changes and the
determination of silver in liver tissue as biomarkers. AgNPs-induced impacts were
recorded in some of these characteristics based on their size (20 and 40 nm) and
their concentration (10 and 100 μg/L). Concentrations of liver enzymes (Aspartic
aminotransferase; AST, Alanine aminotransferase; ALT), alkaline phosphatase (ALP),
total lipids (Tl), Glucose (Glu) and Ag-concentration in liver tissue exhibited a
significant increase under stress in all exposed groups compared to the control
group. The total proteins (Tp), albumin (Al), and globulin (Gl) concentrations
exhibited significantly decrease in all treated groups compared to the control
group. At tissue and cell levels, histopathological changes were observed. These
changes include proliferation of hepatocytes, infiltrations of inflammatory cells,
pyknotic nuclei, cytoplasmic vaculation, melanomacrophages aggregation, dilation in
the blood vessel, hepatic necrosis, rupture of the wall of the central vein, and
apoptotic cells in the liver of AgNPs-exposed fish. As well as the depletion of
glycogen content in the liver (feeble magenta coloration) was observed. The size
and number of melanomacrophage centers (MMCs) in liver tissue showed highly
significant difference in all exposed groups compared to the control group.
Recovery period for 15 days led to improved most alterations in the biochemical,
histopathological, and histochemical parameters induced by AgNPs and AgNO3. In
conclusion, one can assume liver sensitivity of C. garepinus for AgNPs and the
recovery period is a must. © 2020
AU - Naguib, M.
AU - Mahmoud, U. M.
AU - Mekkawy, I. A.
AU - Sayed, A. E. D. H.
DB - Scopus
DO - 10.1016/j.toxrep.2020.01.002
KW - AgNPs
Biochemical
Clarias gariepinus
Hepatocyte
Histochemical
Histopathological
MMCs
albumin
globulin
silver nanoparticle
silver nitrate
animal tissue
Article
behavior assessment
cell aggregation
cell count
cell infiltration
cell proliferation
cell size
controlled study
drug analysis
drug determination
drug exposure
glucose level
histochemistry
histopathology
lipid blood level
liver injury
liver necrosis
liver tissue
liver toxicity
macrophage
melanomacrophage
morphology
mortality
nonhuman
nutritional deficiency
particle size
priority journal
vasodilatation
M3 - Article
PY - 2020
SP - 133-141
ST - Hepatotoxic effects of silver nanoparticles on Clarias gariepinus;
Biochemical, histopathological, and histochemical studies
T2 - Toxicology Reports
TI - Hepatotoxic effects of silver nanoparticles on Clarias gariepinus;
Biochemical, histopathological, and histochemical studies
85077744804&doi=10.1016%2fj.toxrep.2020.01.002&partnerID=40&md5=2f7ad86d1bd17b4143c
41c49fe0a19c7
VL - 7
ID - 5386
ER -
TY - JOUR
AB - Plant intervened synthesis of nanoparticles has attracted an ample amount of
attention because this method is facile, cost-efficient, and eco-friendly. The
present study aims to synthesize AgNPs using Caesalpinia bonducella leaf extract by
employing a green chemistry approach and was characterized. UV-visible spectroscopy
revealed characteristic absorption maxima at 434 nm. FT-IR studies affirmed
presence of phytoconstituents that supports role of metabolites as a reducing and
capping agent. TEM and FESEM analysis reveal nanoparticles are spherical in shape
and EDS confirmed presence of elemental signature of silver at 3 KeV. XRD disclosed
crystalline nature of AgNPs with crystalline size of 15.17 nm and Zeta potential at
-30 mV indicates good stability. AgNPs showed good anti-inflammatory potential.
Anti-cancer activity of AgNPs revealed potential efficacy against MCF-7 cell lines
with 24 h IC50 value of 25 mu g/mL. Conferring results, present reports that the
biological synthesis method was found highly beneficial in fabrication
nanoparticles in medicinal applications.
AN - WOS:000745882900001
AU - Naik, J. R.
AU - David, M.
C6 - JAN 2022
DA - 2022 JAN 3
DO - 10.1080/24701556.2021.2025093
PY - 2022
SN - 2470-1556
2470-1564
ST - Green synthesis of silver nanoparticles using Caesalpinia bonducella leaf
extract: characterization and evaluation of in vitro anti-inflammatory and anti-
cancer activities
TI - Green synthesis of silver nanoparticles using Caesalpinia bonducella leaf
extract: characterization and evaluation of in vitro anti-inflammatory and anti-
cancer activities
ID - 5868
ER -
TY - JOUR
AB - Anti-inflammatory, analgesic, antipyretic, and gastrointestinal ulcerogenic
activities of 2-(8-methyl-10,11-dihydro-11-oxodibenz[b,f]oxepin-2-yl)propionic acid
(AD-1590), a new non-steroidal anti-inflammatory drug, were compared with
indomethacin (INN: indometacin) and other non-steroidal anti-inflammatory drugs
(NSAID) in experimental animals. 1. AD-1590 demonstrated potent inhibitory activity
on acute and subacute inflammation such as carrageenin hind paw edema (oral ED50 =
1.35 mg/kg), acetic acid-induced increased vascular permeability (0.205 mg/kg). UV-
erythema (0.295 mg/kg) and felt pellet-induced granuloma formation (1.7 mg/kg), and
its potency was on the whole 2 to 3 times that of indomethacin. 2. Oral analgesic
ED50-values of AD-1590 were 0.245, 8.32 and 13.9 mg/kg in the writhing tests, and
2.45 mg/kg in the silver nitrate-induced arthritic pain test. Analgesic potency of
AD-1590 was on the whole comparable to that of indomethacin. 3. Against the pyrexia
caused by two kinds of pyrogens (yeast and adjuvant). AD-1590 exerted a strong
antipyretic action at oral doses as low as 0.02 to 0.1 mg/kg, and its potency (ED50
= 0.0210 and 0.0406 mg/kg) was 8.7 to 11 times that of indomethacin. AD-1590
displayed the anti-pyretic activity at low doses which were widely different from
its anti-inflammatory and analgesic effective dose. The body temperature was not
affected by 20 mg/kg p.o. of AD-1590 in afebrile animals. AD-1590 was the strongest
antipyretic drug among 10 NSAID tested. 4. In rats, AD-1590 produced
gastrointestinal ulcer similar to indomethacin, and its gastric ulcerogenicity
(SUD50 = 13.8 mg/kg p.o.) was about one-half that of indomethacin. The activity of
AD-1590 in the fecal occult bleeding test in beagle dogs was weaker than that of
indomethacin. 5. The potency of AD-1590 (IC50 = 0.78 μmol/l) as a prostaglandin
synthetase inhibitor was about 2.7 times that of indomethacin in the in vitro test.
6. The safety index (SUD50/ED50) of AD-1590 was higher than that of indomethacin,
extremely high (the index = 657 and 340) in the antipyretic activity. Acute lethal
toxicity of AD-1590 (oral LD50 = 147 mg/kg in rats, 500 mg/kg in mice) was about
1/8 and 1/24 that of indomethacin. From these results, it was suggested that AD-
1590 had extraordinarily potent antipyretic activity, potent anti-inflammatory
activity superior to indomethacin, analgesic activity equivalent to indomethacin,
and a wide safety margin.
AU - Nakamura, H.
AU - Yokoyama, Y.
AU - Motoyoshi, S.
AU - Ishii, K.
AU - Imazu, C.
AU - Seto, Y.
AU - Kadokawa, T.
AU - Shimizu, M.
DB - Scopus
IS - 11
KW - Animals
Capillary Permeability
Dibenzoxepins
Erythema
Female
Fever
Guinea Pigs
Inflammation
Male
Mice
Pain
Peptic Ulcer
Pleurisy
Prostaglandins
Rats
acetic acid
adjuvant
alclofenac
arachidonic acid c 14
bermoprofen
carrageenan
clidanac
diclofenac
epirizole
flufenamic acid
ibuprofen
indometacin
kaolin
ketoprofen
mefenamic acid
naproxen
new drug
phenylbutazone
phenylquinone
radioisotope
silver nitrate
tiaramide
tolmetin
unclassified drug
analgesia
animal cell
animal experiment
animal model
arthritis
article
body temperature
digestive system
dog
dose response
drug comparison
drug mechanism
drug response
drug safety
drug screening
drug toxicity
edema
fever
guinea pig
intoxication
kidney
mouse
nonhuman
peripheral nervous system
prostaglandin synthetase inhibition
rat
subcutaneous drug administration
subcutaneous tissue
ulcer
yeast
M3 - Article
PY - 1983
SP - 1555-1569
ST - The pharmacological profile of 2-(8-methyl-10,11-dihydro-11-
oxodibenz[b,f]oxepin-2-yl)propionic acid (AD-1590), a new non-steroidal anti-
inflammatory agent with potent antipyretic activity
T2 - Arzneimittel-Forschung/Drug Research
TI - The pharmacological profile of 2-(8-methyl-10,11-dihydro-11-
oxodibenz[b,f]oxepin-2-yl)propionic acid (AD-1590), a new non-steroidal anti-
inflammatory agent with potent antipyretic activity
0021034919&partnerID=40&md5=b783d59f76704110baf4e9b0a23ec3be
VL - 33
ID - 5791
ER -
TY - JOUR
AB - In this study, silver nanoparticles were synthesized (AgNPs) using aqueous
rhizome extract of Acorus calamus (ACRE) and evaluated their in vitro anticancer
activity and in vivo toxicity in a Wistar rat model. The synthesized AgNPs showed
good catalytic activity against different organic pollutant dyes. In vitro
cytotoxic effects of AgNPs were assessed in Hep2, COLO 205 and SH-SY5Y cells using
MTT assay. Further, the apoptotic changes induced by AgNPs in more susceptible Hep2
cells were observed through AO/EB, DCFH-DA, Rhodamine 123, PI/DAPI staining,
oxidative stress markers and Western blotting. In vivo toxicity study revealed
substantial alterations in the levels of serum biochemical markers including AST,
ALT, LDH and inflammatory markers such as TNF-alpha and IL-6 on day 29 when rats
treated with AgNPs as compared to control, however, these levels were restored to
normal at the end of washout period on day 89. No remarkable changes were observed
in liver oxidative stress enzymes. ICP-OES analysis indicated bio-distribution of
silver in spleen (5.67 mu g/g) and liver (4.98 mu g/g) in rats treated with 10
mg/kg b.w of AgNPs on day 29 and elimination of silver from all organs was observed
at the end of washout period on day 89. Histopathological analysis revealed no
significant changes in kidney, spleen, lungs, heart, testis and brain with 5 and 10
mg/kg b.w of AgNP. However, 10 mg/kg b.w of AgNPs showed moderate degree of cell
swelling and vacuolar degeneration in liver and these alterations were reverted
back to normal at the end of washout period. Findings from this study signify green
synthesized AgNPs at low concentrations might be useful in many ways with
ecofriendly nature.
AN - WOS:000442192000038
AU - Nakkala, J. R.
AU - Mata, R.
AU - Raja, K.
AU - Chandra, V. K.
AU - Sadras, S. R.
DA - OCT 1
DO - 10.1016/j.msec.2018.05.048
PY - 2018
SN - 0928-4931
1873-0191
SP - 372-381
ST - Green synthesized silver nanoparticles: Catalytic dye degradation, in vitro
anticancer activity and in vivo toxicity in rats
TI - Green synthesized silver nanoparticles: Catalytic dye degradation, in vitro
VL - 91
ID - 5988
ER -
TY - JOUR
AB - In this study, silver nanoparticles were synthesized (AgNPs) using aqueous
rhizome extract of Acorus calamus (ACRE) and evaluated their in vitro anticancer
activity and in vivo toxicity in a Wistar rat model. The synthesized AgNPs showed
good catalytic activity against different organic pollutant dyes. In vitro
cytotoxic effects of AgNPs were assessed in Hep2, COLO 205 and SH-SY5Y cells using
MTT assay. Further, the apoptotic changes induced by AgNPs in more susceptible Hep2
cells were observed through AO/EB, DCFH-DA, Rhodamine 123, PI/DAPI staining,
oxidative stress markers and Western blotting. In vivo toxicity study revealed
substantial alterations in the levels of serum biochemical markers including AST,
ALT, LDH and inflammatory markers such as TNF-α and IL-6 on day 29 when rats
treated with AgNPs as compared to control, however, these levels were restored to
normal at the end of washout period on day 89. No remarkable changes were observed
in liver oxidative stress enzymes. ICP-OES analysis indicated bio-distribution of
silver in spleen (5.67 μg/g) and liver (4.98 μg/g) in rats treated with 10 mg/kg
b.w of AgNPs on day 29 and elimination of silver from all organs was observed at
the end of washout period on day 89. Histopathological analysis revealed no
significant changes in kidney, spleen, lungs, heart, testis and brain with 5 and 10
mg/kg b.w of AgNP. However, 10 mg/kg b.w of AgNPs showed moderate degree of cell
swelling and vacuolar degeneration in liver and these alterations were reverted
back to normal at the end of washout period. Findings from this study signify green
synthesized AgNPs at low concentrations might be useful in many ways with
ecofriendly nature. © 2018 Elsevier B.V.
AU - Nakkala, J. R.
AU - Mata, R.
AU - Raja, K.
AU - Khub Chandra, V.
AU - Sadras, S. R.
DB - Scopus
DO - 10.1016/j.msec.2018.05.048
KW - Anti-cancer activity
Bio distribution
Catalytic activity
Nano silver
Toxicity
Acorus
Animals
Biomarkers, Tumor
Catalysis
Cell Line, Tumor
Fluorescent Dyes
Humans
Male
Metal Nanoparticles
Neoplasm Proteins
Neoplasms
Organ Specificity
Plant Extracts
Rats
Rats, Wistar
Rhizome
Silver
Biochemistry
Cytotoxicity
Metal nanoparticles
Organic pollutants
fluorescent dye
metal nanoparticle
plant extract
silver
tumor marker
tumor protein
Biochemical markers
Biodistributions
Cytotoxic effects
Histopathological analysis
Low concentrations
Oxidative stress markers
animal
antibody specificity
catalysis
chemistry
drug effect
human
male
metabolism
neoplasm
pathology
rat
rhizome
tumor cell line
Wistar rat
Catalyst activity
M3 - Article
PY - 2018
SP - 372-381
ST - Green synthesized silver nanoparticles: Catalytic dye degradation, in vitro
TI - Green synthesized silver nanoparticles: Catalytic dye degradation, in vitro
85047271819&doi=10.1016%2fj.msec.2018.05.048&partnerID=40&md5=82b79f1b625e44b83fce5
88451e0842f
VL - 91
ID - 5451
ER -
TY - JOUR
AB - Background: Colorectal carcinogenesis is one of the most common
cancers/lethal diseases. Chronic inflammation is considered a risk factor for
colorectal cancer. Hesperetin, a flavonone found in citrus fruits and oranges is
shown to possess potent growth inhibitory effects against various human cancer
cells. It possesses anti-inflammatory and antioxidant properties. Aim of the scope:
In the present study, we have evaluated the chemopreventive efficacy of hesperetin
against rat colon carcinogenesis in male Wistar rats. Methods: Group 1 served as
control, received modified pellet diet and group 2 rats received 20 mg/kg body
weight of hesperetin p.o. every day. Groups 36 rats were given subcutaneous
injections of 1,2-dimethylhydrazine (DMH, 20 mg/kg body weight) once a week for 15
consecutive weeks. In addition, rats in group 4 received hesperetin as in group 2
for the first 15 weeks (initiation), group 5 rats received hesperetin as in group 2
after the last injection of DMH and continued till the end of the experimental
period (postinitiation). Group 6 rats received hesperetin as in group 2 throughout
the entire experimental period of 32 weeks. Results: Detection of cell
proliferation markers such as proliferating cell nuclear antigen (PCNA)
(immunohistochemistry), argyrophilic nucleolar organizer regions (AgNORs) (silver
staining); apoptosis (immunoblotting and immunohistochemistry); angiogenic growth
factors (ELISA) indicated decreased cell proliferation and increased apoptotic
markers in the colon. In addition, decreased angiogenic growth factors such as
vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF),
epidermal growth factor (EGF), and downregulation of mRNA Cyclooxygenase-2 (COX-2)
expressions were observed in mucosal and fecal samples of hesperetin-supplemented
rats. Conclusions: Hesperetin supplementation showed an inhibition of cell
proliferation markers, angiogenic growth factors, COX-2 mRNA expression and
induction of apoptosis. Thus, hesperetin can be used as a potent chemopreventive
agent against DMH-induced colon cancer. © 2012 Informa Healthcare USA, Inc.
AU - Nalini, N.
AU - Aranganathan, S.
AU - Kabalimurthy, J.
DB - Scopus
DO - 10.3109/15376516.2012.673092
IS - 5
KW - angiogenesis
DMH
Hesperetin
inflammation
1,2-Dimethylhydrazine
Animals
Apoptosis
Blotting, Western
Cell Proliferation
Colonic Neoplasms
Cyclooxygenase 2
Hesperidin
Male
Rats
Rats, Wistar
Citrus
Rattus
Rattus norvegicus
1,2 dimethylhydrazine
cycline
cyclooxygenase 2
hesperetin
protein Bax
protein bcl 2
vasculotropin
animal model
animal tissue
apoptosis
argentaffin cell
article
cancer inhibition
cancer prevention
cell proliferation
chemoprophylaxis
colon carcinogenesis
colon mucosa
controlled study
dose response
down regulation
drug efficacy
evaluation
gene targeting
immunoblotting
male
nonhuman
priority journal
protein expression
rat
silver staining
treatment duration
M3 - Article
PY - 2012
SP - 397-408
ST - Chemopreventive efficacy of hesperetin (citrus flavonone) against 1,2-
dimethylhydrazine-induced rat colon carcinogenesis
T2 - Toxicology Mechanisms and Methods
TI - Chemopreventive efficacy of hesperetin (citrus flavonone) against 1,2-
dimethylhydrazine-induced rat colon carcinogenesis
84860856963&doi=10.3109%2f15376516.2012.673092&partnerID=40&md5=a23f5176765869f51cb
af63bba4b434c
VL - 22
ID - 5692
ER -
TY - JOUR
AB - Starting from the proligand 2-[(6-methylpyridin-2-yl)] imidazo[1,5-a]
pyridin-4-ylium hexafluorophosphate(1 center dot PF6), three new complexes, viz.
[Au(1)(2)] [PF6] (2), [1/2AuCl(2), 1/2AuCl(4)](-) [(1H)](+) (3), and [Au(1)Cl-3]
(4), have been synthesized and characterized employing different spectroscopic
methods. [Au(1)Cl-3] (4) has been synthesized by the disproportionation process.
During the transformation of 2 to 4, the annelated proligand stabilizes both Au(I)
and Au(III), and the isolation of the intermediate (3) confirms the conversion of
Au(I) - Au(III) through the disproportionation pathway. The solid state structures
of 2, 3 and 4 have been determined. Linear geometry was observed in 2, whereas
complex 4 adopted square-planar geometry. Gold complexes 2 and 4 have been
subjected to growth inhibitory studies. Complex 2 induced apoptosis in HepG2 cells
along with increased expression of proteins involved in the mitochondrial death
pathway, suggesting that apoptosis may occur via the mitochondrial death pathway.
AN - WOS:000385869600079
AU - Nandy, A.
AU - Samanta, T.
AU - Mallick, S.
AU - Mitra, P.
AU - Seth, S. K.
AU - Das Saha, K.
AU - Al-Deyabe, S. S.
AU - Dinda, J.
DA - JUL 1
DO - 10.1039/c5nj02979a
IS - 7
PY - 2016
SN - 1144-0546
1369-9261
SP - 6289-6298
ST - Synthesis of gold(III) <- gold(I)-NHC through disproportionation: the role of
gold(I)-NHC in the induction of apoptosis in HepG2 cells
TI - Synthesis of gold(III) <- gold(I)-NHC through disproportionation: the role of
gold(I)-NHC in the induction of apoptosis in HepG2 cells
VL - 40
ID - 6551
ER -
TY - JOUR
AB - Wound healing, a complex biological process, has attained a lot of attention
as dermatologists are primarily interested in stimulated wound closure without
formation of scar or a faint scar. The recent upsurgence of nanotechnology has
provided novel therapeutic materials in the form of silver and gold nanoparticles
which accelerate the wound healing process. The effect of formulated nanoparticles
using Coleus forskohlii root extract (green synthesized) has been tried out for
ameliorating full thickness excision wounds in albino Wistar male rats. The
evaluation of in vivo activity of nanoparticles in wound healing was carried out on
open wounds made by excision on the dorsal sides of albino Wistar rats under
anesthesia, and the healing of the wounds was assessed. Histological aspects of the
healing process were studied by a HE (Hematoxylin and Eosin) staining method to
assess various degrees of re-epithelialization and the linear alignment of the
granulation tissue whereas Van Gieson's histochemical staining was performed to
observe collagen fibers. The healing action shown by the formulated nanoparticles
was remarkable during the early stages of wound healing, which resulted in the
substantial reduction of the whole healing period. Topical application of
formulated gold nanoparticles was found to be more effective in suppressing
inflammation and stimulating re-epithelialization compared to silver nanoparticles
during the healing process. The results throw light on the amelioration of excision
wounds using nanoparticles which could be a novel therapeutic way of improving
wound healing in clinical practice. The mechanism of advanced healing action of
both types of nanoparticles could be due to their antimicrobial, antioxidant and
anti-inflammatory properties. (C) 2016 Elsevier B.V. All rights reserved.
AN - WOS:000372759100036
AU - Naraginti, S.
AU - Kumari, P. L.
AU - Das, R. K.
AU - Sivakumar, A.
AU - Patil, S. H.
AU - Andhalkar, V. V.
DA - MAY 1
DO - 10.1016/j.msec.2016.01.069
PY - 2016
SN - 0928-4931
1873-0191
SP - 293-300
ST - Amelioration of excision wounds by topical application of green synthesized,
formulated silver and gold nanoparticles in albino Wistar rats
TI - Amelioration of excision wounds by topical application of green synthesized,
formulated silver and gold nanoparticles in albino Wistar rats
VL - 62
ID - 6367
ER -
TY - JOUR
AB - Colorectal cancer (CRC) progression is a complex process, with an interplay
of multiple molecular signaling pathways. Cyclooxygenase-2 (COX-2) and NF-κB are
important hallmark proteins responsible for the transition from inflammation to
colon cancer. Due to the adverse effects of chemotherapeutic drugs, there is an
imperative need to develop new drugs, and recently, nanoparticles found to be
promising strategy in tumor detection, prevention and treatment of cancer.
Biosynthesis of silver nanoparticles (AgNPs) was achieved with the help of
Eucalyptus globulus L. leaf extract. Using a typical XRD pattern, NanoSight and TEM
technique, the size and shape of the biogenic AgNPs were determined as ~ 20 nm and
spherical. The cytotoxicity study exhibited a dose-dependent effect against HCT116
cells, with an inhibitory concentration (IC50) of 1.152 µg/ml. In addition, AgNPs
effectively inhibited the proliferation, colony formation, with increased ROS
production compared to untreated cells. Further, mechanistic analysis revealed that
AgNPs arrested the cell cycle, downregulated the expression of antiapoptotic,
inflammatory, stem cell markers, and upregulated the apoptotic genes in HCT116
cells. In conclusion, for the first time, we report the green synthesis of AgNPs
using E. globulus leaf extract that has potential anticancer activity with dual
inhibitory action on COX-2 and NF-κB expression. © 2021, The Author(s), under
exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
AU - Narasimha, V. R.
AU - Latha, T. S.
AU - Pallu, R.
AU - Panati, K.
AU - Narala, V. R.
DB - Scopus
DO - 10.1007/s10876-021-02143-z
IS - 5
Colorectal cancer
Nanomedicine
Toxicity
M3 - Article
PY - 2022
SP - 2215-2231
ST - Anticancer Activities of Biogenic Silver Nanoparticles Targeting Apoptosis
and Inflammatory Pathways in Colon Cancer Cells
TI - Anticancer Activities of Biogenic Silver Nanoparticles Targeting Apoptosis
and Inflammatory Pathways in Colon Cancer Cells
85112761273&doi=10.1007%2fs10876-021-02143-
z&partnerID=40&md5=37e710f06a4b8cdb2251181d51dee939
VL - 33
ID - 5094
ER -
TY - JOUR
AB - The green-mediated synthesis of copper nanoparticles is of great interest in
nanotechnology and is regarded as a low-cost and environmentally beneficial method.
Herein, Emilia sonchifolia leaf extract was employed as a reducing and capping
agent for the green production of copper nanoparticles. In this work, we focused on
the in vivo and in vitro biological studies of copper nanoparticles, which were
evaluated in zebrafish (Danio rerio) embryos. The biological effects from the in
vitro studies of the copper nanoparticles included cytotoxicity (in human cells)
and anti-diabetic, anti-inflammatory, and antibacterial activity. Furthermore, the
effectiveness of the greenly produced copper nanoparticles for photocatalysis was
also evaluated, and then SEM-EDX, FTIR, XRD, TGA and UV-vis spectroscopy were used
to characterise the copper nanoparticles. The results of the toxicity test on
zebrafish embryos demonstrated that the green-produced copper nanoparticles had a
significantly low harmful effect. According to the results, the copper
nanoparticles showed dose-dependent cytotoxicity against human keratinocytes
(HaCaT) and human breast cancer cells (MCF-7), which was higher than that of the
Emilia sonchifolia leaf extract. The green copper nanoparticles also demonstrated
more potent anti-inflammatory, antibacterial and anti-diabetic properties. In the
photocatalytic experiment, the produced copper nanoparticles successfully degraded
the organic methylene blue dye. Thus, it can be concluded that copper nanoparticles
can be employed for drug administration in both in vitro and in vivo settings in
biomedical applications. Additionally, as catalysts, these copper nanoparticles can
be employed for the removal of organic dyes.
AN - WOS:000999719900001
AU - Narayanan, V. P. S.
AU - Kathirason, S. G.
AU - Elango, P.
AU - Subramanian, R.
AU - Sivagurusundar, R.
AU - Gurusamy, A.
DA - MAY 30
DO - 10.1039/d3ra00454f
IS - 24
PY - 2023
SN - 2046-2069
SP - 16724-16740
ST - Emilia sonchifolia leaf extract-mediated green synthesis, characterization,
in vitro biological activities, photocatalytic degradation and in vivo Danio rerio
embryo toxicity of copper nanoparticles
T2 - RSC ADVANCES
TI - Emilia sonchifolia leaf extract-mediated green synthesis, characterization,
in vitro biological activities, photocatalytic degradation and in vivo Danio rerio
embryo toxicity of copper nanoparticles
VL - 13
ID - 6342
ER -
TY - JOUR
AB - Background: Fruits are an important dietary component, which supply vitamins,
minerals, as well as dietary fiber. In addition, they are rich sources of various
biological and pharmacologically active compounds. Among these, temperate fruits
are well studied for their pharmacological potentials, whereas tropical/subtropical
fruits are less explored for their health impacts. In India, most of the consumed
fruits are either tropical or subtropical. Objectives: This mini review aims to
provide a health impact of major tropical and sub-tropical fruits of India,
emphasizing their anticancer efficacy. In addition, the identified bioactive
components from these fruits exhibiting anticancer efficacy are also discussed
along with the patent literature published. Methods: The literature was collected
from various repositories, including NCBI, ScienceDirect, Eurekaselect, and Web of
Science; literature from predatory journals was omitted during the process. Patent
literature was collected from google patents and similar patent databases. Results:
Tropical fruits are rich sources of various nutrients and bioactive components
including polyphenols, flavonoids, anthocyanin, etc. By virtue of these
biomolecules, tropical fruits have been shown to interfere with various steps in
carcinogenesis, metastasis, and drug resistance. Their mode of action is either by
activation of apoptosis, regulation of cell cycle, inhibition of cell survival and
proliferation pathways, increased lipid trafficking or inhibiting inflammatory
pathways. Several molecules and combinations have been patented for their
anticancer and chemoprotective properties. Conclusion: Overall, the present
concludes that Indian tropical/ subtropical fruits are nutritionally and
pharmacologically active and may serve as a source of novel anticancer agents in
the future. © 2022 Bentham Science Publishers.
AU - Narayanankutty, A.
DB - Scopus
DO - 10.2174/1574892816666211130165200
IS - 2
bioactive compounds
cancer
degenerative diseases
Indian tropical fruits
Fruit
Humans
Nutritive Value
Patents as Topic
Plant Extracts
Polyphenols
acetogenin
Ananas comosus extract
Annona Squamosa extract
annonacin
anthocyanin
artocarpanone
artocarpin
Artocarpus altilis extract
Artocarpus heterophyllus extract
ascorbic acid
benzyl glucosinolate
beta carotene
bullatacin
caffeic acid
carbohydrate
Carica papaya extract
carotenoid
catechin
chlorogenic acid
coumaric acid
cyanidin
cyanomaclurin
cycloartocarpin
epicatechin
epigallocatechin
ferulic acid
gallic acid
Garcinia cambogia extract
gigantecin
guava extract
Hermes antigen
jacalin
malvidin chloride
mangiferin
mangostin
mangsterol
Manilkara zapota extract
moracin
Morinda citrifolia extract
motrilin
Musa acuminata cancer
Musa paradisiaca extract
myricetin
Nephelium litchi extract
nicotinamide
petunidin
plant extract
polyketide
polyphenol
pomegranate extract
protein
pterostilbene
pyrogallol
riboflavin
Semen coicis extract
silver nanoparticle
sinecatechins
Solanum nigrum extract
squamocin
syringic acid
Syzygium cumini extract
thiamine
unclassified drug
uvaricin
vanillic acid
vimentin
Zihiphud jujube extract
A-549 cell line
angiogenesis
Article
ascites hepatoma
ascites tumor
ash
breast cancer
breast cancer cell line
Caco-2 cell line
cancer growth
cancer stem cell
cancer therapy
carcinogenesis
cell cycle
cell cycle arrest
cell proliferation
cell survival
colon cancer
colon cell line
drug activity
drug mechanism
endometrium
endotoxemia
fruit
H22 cell line
HCT 116 cell line
Hep-G2 cell line
HT-29 cell line
human
India
leukemia cell
liver cell line
lung cell line
MCF-7 cell line
medicinal plant
melanoma cell
metastasis
nonhuman
nutritional value
oxidative stress
pharyngeal cell line
phytochemistry
prostate cell line
RNA sequence
skin cancer
stomach cancer
subtropical fruit
tropical fruit
patent
M3 - Article
PY - 2022
SP - 124-135
ST - Pharmacological Potentials and Nutritional Values of Tropical and Subtropical
Fruits of India: Emphasis on their Anticancer Bioactive Components
T2 - Recent Patents on Anti-Cancer Drug Discovery
TI - Pharmacological Potentials and Nutritional Values of Tropical and Subtropical
Fruits of India: Emphasis on their Anticancer Bioactive Components
85129191905&doi=10.2174%2f1574892816666211130165200&partnerID=40&md5=ad71b8f8ef9827
b0810f720bbb1b0d96
VL - 17
ID - 5110
ER -
TY - JOUR
AB - Recently, green nanotechnology got great attention due to their reliable,
sustainable, and eco-friendly synthesis protocols. The green nanoparticles (GNPs)
are preferred over chemically synthesized nanoparticles owing to less destructive
effects associated with the synthesis procedures as well as therapeutic
involvement. In this review, we have discussed the applications of GNPs in
inflammation-mediated disorders, with special emphasis on cancer, initiated due to
oxidative stress and inflammatory cascade. Real-time mechanism based studies on
GNPs have suggested their anticancer effects through inducing apoptosis, inhibiting
angiogenesis, tissue invasion metastasis, reduced replicative capabilities in
addition to target specific different signaling molecules and cascades involved in
the development or progression of cancer. Moreover, the association of GNPs with
the inhibition or induction of autophagy for the management of cancer has also been
discussed. A large number of studies showed the GNPs have multifunctional
biomedical properties of theranostic prominence. Therefore, the development of GNPs
with naturally established systems could upsurge their definite applications as
biomedicines including target specific destruction of the cancerous cells. © 2022
Elsevier Ltd
AU - Naseer, F.
AU - Ahmed, M.
AU - Majid, A.
AU - Kamal, W.
AU - Phull, A. R.
DB - Scopus
DO - 10.1016/j.semcancer.2022.06.014
KW - Autophagy
Cancer
Green nanoparticles
Inflammation
Molecular mechanism
Nanomedicine
Apoptosis
Gold
Humans
Metal Nanoparticles
Neoplasms
ABC transporter
antiinfective agent
cyclin dependent kinase inhibitor
green nanoparticle
microRNA
nanoparticle
silver nanoparticle
tumor necrosis factor receptor associated factor 2
unclassified drug
vasculotropin
gold
metal nanoparticle
anemia
angiogenesis
antifungal activity
apoptosis
Aspergillus flavus
autophagy (cellular)
biomedicine
carcinogenesis
CD8+ T lymphocyte
cell cycle arrest
cell migration
cell proliferation
cell survival
constipation
DNA methylation
DNA replication
drug safety
Fusarium oxysporum
gene therapy
green chemistry
HT-1080 cell line
incubation time
MCF-7 cell line
metastasis
nanomedicine
nonhuman
oxidative stress
Parkinson disease
phagocytosis
Pi3K/Akt signaling
Review
Stenotrophomonas maltophilia
systematic review
tea
chemistry
human
neoplasm
M3 - Review
PY - 2022
SP - 310-324
ST - Green nanoparticles as multifunctional nanomedicines: Insights into anti-
inflammatory effects, growth signaling and apoptosis mechanism in cancer
T2 - Seminars in Cancer Biology
TI - Green nanoparticles as multifunctional nanomedicines: Insights into anti-
inflammatory effects, growth signaling and apoptosis mechanism in cancer
85133851288&doi=10.1016%2fj.semcancer.2022.06.014&partnerID=40&md5=d747450dc00dafae
0132a076bed4cc5d
VL - 86
ID - 4969
ER -
TY - JOUR
AB - Oxidative stress is a destructive phenomenon that affects various cell
structures including membranes, proteins, lipoproteins, lipids, and DNA. Oxidative
stress and inflammation owing to lifestyle changes may lead to serious diseases
such as Cancers, Gout, and Arthritis etc. These disorders can be prevented using
different therapeutic strategies including nanomedicine. Biosynthesized gold
nanoparticles (GNPs) because of their anti-inflammatory and antioxidant
bioactivities can be key player in reversal of these ailments. This study was
carried out to evaluate the anti-inflammatory and antioxidant potential of bio
fabricated GNPs with Sarcococca saligna (S. saligna) extract on injured human
adipose-derived Mesenchymal stem cells (hADMSCs). GNPs were characterized by
ultraviolet-visible (UV-Vis) spectroscopy, Scanning Electron Microscopy (SEM), x-
ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and energy
dispersive x-ray (EDS). Phytochemical screening of biosynthesized GNPs exhibited a
significant release of polyphenols, that is, total phenolic content (TPC) and total
flavonoid content (TFC). GNPs priming amended the in vitro injury caused by
Monosodium Iodoacetate (MIA) as exhibited by improved cell viability, wound closure
response and superoxide dismutase activity (SOD). The anti-inflammatory conduct
assessed through NF-kappa B pathway and other associated inflammatory markers
reported down-regulation of TNF-alpha (0.644 +/- 0.045), IL-1 beta (0.694 +/-
0.147) and IL-6 (0.622 +/- 0.112), apoptosis causing genes like Caspase-3 (0.734
+/- 0.13) and BAX (0.830 +/- 0.12), NF-kappa B pathway, p65 (0.672 +/- 0.084) and
p105 (0.539 +/- 0.083) associated genes. High SOD activity (95 +/- 5.25%) revealed
by treated hADMSCs with GNPs also supported the antioxidant role of GNPs in vitro
model. This study concludes that S. saligna bio fabricated GNPs priming may improve
the therapeutic potential of hADMSCs against chronic inflammatory problems by
regulating NF-kappa B pathway.
AN - WOS:001045517100001
AU - Naseer, N.
AU - Mustafa, M. M.
AU - Latief, N.
AU - Fazal, N.
AU - Tariq, M.
AU - Afreen, A.
AU - Yaqub, F.
AU - Riazuddin, S.
C6 - AUG 2023
DA - 2023 AUG 10
DO - 10.1002/jbm.b.35303
PY - 2023
SN - 1552-4973
1552-4981
ST - Sarcococca saligna fabricated gold nanoparticles alleviated in vitro
oxidative stress and inflammation in human adipose-derived stem cells
TI - Sarcococca saligna fabricated gold nanoparticles alleviated in vitro
oxidative stress and inflammation in human adipose-derived stem cells
ID - 6383
ER -
TY - JOUR
AB - Hepatitis C virus is a major causative agent of chronic liver disease. Viral
genotype, mutations, virus-host interaction, expression of viral proteins and host
immune-reaction are important factors in the pathogenesis of HCV infection. Precise
pathogenesis and perpetuation of hepatocellular injury in hepatitis C viral
infection remain unclear. Proposed mechanisms include direct viropathic effect, the
host immune response mediated through cytotoxic T lymphocytes, both viropathic and
cytopathic effects, and macrophages/monocytes. Apoptosis occurs both in acute or
chronic hepatitis and has been suggested to be mediated through Fas antigen. In HCV
infection, Fas expression is up-regulated in the liver cells in line with the
severity of liver inflammation. When HCV-specific T cells migrate into hepatocytes
and recognize the viral antigen via the T cell receptor, they become activated and
express Fas ligand that transduces the apoptotic death signal to Fas-bearing
hepatocytes resulting in their destruction. Thus, the Fas system plays an important
role in liver cell injury by HCV infection. Possible inducers of apoptosis in
hepatitis C include cytokines, especially tumor necrosis factor-alpha (TNF-alpha),
released by inflammatory cells, and acting through TNF and other cytokine
receptors.
AN - WOS:000086167600037
AU - Nasir, A.
AU - Arora, H. S.
AU - Kaiser, H. E.
DA - JAN-FEB
IS - 1
PY - 2000
SN - 0258-851X
SP - 297-300
ST - Apoptosis and pathogenesis of viral hepatitis C - An update
T2 - IN VIVO
TI - Apoptosis and pathogenesis of viral hepatitis C - An update
VL - 14
ID - 6404
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have become increasingly popular in the
biomedical field over the last few decades due to its proven antibacterial
property. Previous scientific studies have reported that one of the major organs
responsible for detoxification of AgNPs is the liver. The liver is also the primary
organ responsible for secretion of angiotensinogen (AGT), a key signaling molecule
involved in the renin-angiotensin system (RAS), which plays an important role in
maintaining cardiac output and vascular pressure. The aim of this study was to
assess any potential changes in the RAS-associated gene signaling, inflammatory
response, and hepatocellular toxicity resulting from AgNP exposure. To do this, 6-
week-old, male Wistar rats were exposed to a subacute inhalation exposure of AgNP
(200 ppb/days over 4 h/days exposure, for 5 d) and their livers were analyzed for
alterations in RAS components, inflammation, and oxidative stress. Real time qPCR
analysis showed that AgNP-exposure resulted in a significant increase in hepatic
AGT, angiotensin converting enzyme (ACE)-1, and ACE-2 mRNA expression. Expression
of inflammatory markers interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-
α were also upregulated with AgNP-exposure, compared to controls. Furthermore AgNP-
exposure mediated a significant increase in hepatic expression of catalase, and
superoxide dismutase, and oxidative stress, as assessed via 8-Oxo-2′-deoxyguanosine
staining. Increased oxidative stress was associated with increased
monocyte/macrophage-2 staining in the liver of AgNP-exposed rats. Such findings
indicate that subacute inhalation exposure to AgNPs mediate increased hepatic RAS
signaling, associated with inflammation, macrophage infiltration, and oxidative
stress. © 2021 Wiley Periodicals LLC.
AU - Nayek, S.
AU - Lund, A. K.
AU - Verbeck, G. F.
DB - Scopus
DO - 10.1002/tox.23412
IS - 3
KW - Animals
Inflammation
Inhalation Exposure
Male
Metal Nanoparticles
Oxidative Stress
Rats
Rats, Wistar
Renin-Angiotensin System
Silver
Cell death
Detoxification
Enzymes
Macrophages
Metal nanoparticles
Oxidative stress
Pathology
Signaling
angiotensin converting enzyme 1
angiotensin converting enzyme 2
catalase
cytochrome P450 1A1
dipeptidyl carboxypeptidase
interleukin 1beta
interleukin 6
messenger RNA
silver nanoparticle
unclassified drug
metal nanoparticle
silver
Biomedical fields
Hepatocellular
Hepatocellular toxicity
Inhalation exposure
Renin-angiotensin system
Scientific studies
Signaling molecules
Wistar rat
detoxification
induced response
nanoparticle
oxidative stress
pollution exposure
rodent
animal experiment
animal model
animal tissue
Article
cell infiltration
controlled study
DNA damage
gene expression
immune response
inflammation
liver fibrosis
liver toxicity
macrophage
male
monocyte
nonhuman
rat
receptor upregulation
renin angiotensin aldosterone system
signal transduction
adverse event
animal
exposure
Toxicity
M3 - Article
PY - 2022
SP - 457-467
ST - Inhalation exposure to silver nanoparticles induces hepatic inflammation and
oxidative stress, associated with altered renin-angiotensin system signaling, in
Wistar rats
TI - Inhalation exposure to silver nanoparticles induces hepatic inflammation and
oxidative stress, associated with altered renin-angiotensin system signaling, in
Wistar rats
85119180312&doi=10.1002%2ftox.23412&partnerID=40&md5=c7e2789f1055d72c5d78c85d370944
21
VL - 37
ID - 5090
ER -
TY - JOUR
AB - Currently, metal nanostructures are widely used in medical, microbiological,
and veterinary practice. Silver nanoparticles are especially promising as
antimicrobial agents, becauseno published data regarding antimicrobial resistance
are available. Whiledeveloping preparations based on metal nanoparticles, an
important remainingissue is the choice of a stabilizer, introduction of which
during the synthesis ensures the preservation of structures at the nanoscale range,
and, consequently, relevant main characteristics, including biocidal properties.
The object of the study was to investigate silver nanoparticle aqueous dispersions
stabilized by natural and synthetic polymeric compounds. Routine strains of Gram-
positive and Gram-negative bacteria were used as experimental models: S. aureus 209
P, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 3177 (O-form), Klebsiella
pneumoniae ATCC 31488, obtained from the Scientific Centre for Expert Evaluation of
Medicinal Products. The antimicrobial activity of diverse variants of silver
nanoparticle aqueous dispersions was assessed by serial dilution platingon dense
nutrient medium. In this work, we examined no effect of silver nanoparticles
without stabilizers, because their absence led to rapid agglomeration of
nanostructures and loss of nanoscale characteristics. The highest sensitivity of
Gram-positive and Gram-negative bacteria was foundto the action of ansilver
nanoparticle aqueous dispersions stabilized by polyazolidinammoniumand modified
with iodine hydrate ions. Drug working concentrations ranging from 0.5 to 3% had a
bactericidal effect against pathogens of purulent-inflammatory diseases, and the
minimum working concentration of 0.125% led to decreased colony-forming units by
20-57% for diverse bacterial strains. Silver nanoparticles stabilized with sodium
dodecyl sulfate showed high efficiency against the studied test strainsprobably due
to the high toxicity of the stabilizer used as was previously established during a
comprehensive safety assessment using biotest objects and cell cultures. In this
regard, its use as a component of antimicrobial preparations is not preferred. The
results of the studies showed that among the variants of silver nanoparticle
aqueous dispersions, preparations stabilized with polyvinyl alcohol and
polyazolidinammonium modified with iodine hydrate ions are the most promising for
use in biomedical practice, because they demonstrate a high level of antibacterial
activity against both Gram-positive and Gram-negative bacteria as causative agents
of purulent-inflammatory diseases and a low toxicity level. This allows us to
recommend them as safe and effective antimicrobial components indisinfectants, as
well as antiseptic preparations for prevention and treatment of skin and soft
tissue infectious diseases. © 2022 Saint Petersburg Pasteur Institute. All rights
reserved.
AU - Nechaeva, O. V.
AU - Shulgina, T. A.
AU - Zubova, K. V.
AU - Glinskaya, E. V.
AU - Bespalova, N. V.
AU - Darin, N. I.
AU - Tichomirova, E. I.
AU - Afinogenova, A. G.
DB - Scopus
DO - 10.15789/2220-7619-AAO-1937
IS - 4
KW - antibiotic resistance
aqueous dispersions
causative agents of purulent-inflammatory diseases
polyazolidinammonium
polymers
ammonium derivative
iodine
nanomaterial
polymer
polyvinyl alcohol
pyrrole derivative
silver nanoparticle
aqueous solution
Article
bacterial strain
bacterium culture
dilution
Escherichia coli
hydration
infectious agent
inflammatory disease
nonhuman
nutrient
Proteus mirabilis
risk assessment
skin infection
soft tissue
soft tissue infection
M3 - Article
PY - 2022
SP - 755-764
ST - АНТИМИКРОБНАЯ АКТИВНОСТЬ ВОДНЫХ ДИСПЕРСИЙ НАНОЧАСТИЦ СЕРЕБРА В ОТНОШЕНИИ
ВОЗБУДИТЕЛЕЙ ГНОЙНО-ВОСПАЛИТЕЛЬНЫХ ЗАБОЛЕВАНИЙ
T2 - Russian Journal of Infection and Immunity
TI - ANTIMICROBIAL ACTIVITY of AQUEOUS DISPERSIONS of SILVER NANOPARTICLES against
PATHOGENS of PURULENT-INFLAMMATORY DISEASES
85140017238&doi=10.15789%2f2220-7619-AAO-
1937&partnerID=40&md5=0a3cbb3b8505355cc1e4c0a3a06dfb47
VL - 12
ID - 5138
ER -
TY - JOUR
AB - The biogenic manufacture of nanoparticles utilising endophytic fungus is an
eco-friendly, cost-effective, and secure alternative to constructing chemical
methods. The prime focus of the study was to fabricate ZnONPs using the biomass
filtrate of endophytic Xylaria arbuscula isolated from Blumea axillaris Linn. and
to evaluate their biological properties. The characterisation of the biosynthesized
ZnO-NPs was done utilising both spectroscopic and microscopic methods. The
bioinspired NPs showed a surface plasmon peak at 370 nm; SEM and TEM micrographs
illustrated the hexagonal organisation; XRD spectra proved the crystalline phase as
hexagonal wurtzite; EDX analysis confirmed the presence of zinc and oxygen atoms;
and the zeta potential analysis proved the stability of ZnONPs. In addition, they
also demonstrated significant concentration-dependent inhibition of antimicrobial,
antioxidant, anti-inflammatory, and antidiabetic potential in comparison with the
reference drugs. In vitro cytotoxicity and wound healing potential of ZnONPs were
examined in L929 cell lines, illustrating that they accelerated the wound healing
process by roughly 95.37 & PLUSMN; 1.12% after a 24-h exposure to ZnONPs. The
photocatalytic activity of the ZnONPs was examined by degrading the methylene blue
dye under solar irradiation. In conclusion, our outcomes showed that
mycosynthesized ZnONPs possessed potent bioactivity and could be an excellent
choice for biomedical applications.
AN - WOS:001026234200001
AU - Nehru, L.
AU - Kandasamy, G. D.
AU - Sekar, V.
AU - Alshehri, M. A.
AU - Panneerselvam, C.
AU - Alasmari, A.
AU - Kathirvel, P.
DA - DEC 31
DO - 10.1080/21691401.2023.2232654
IS - 1
PY - 2023
SN - 2169-1401
2169-141X
SP - 318-333
ST - Green synthesis of ZnO-NPs using endophytic fungal extract of Xylaria
arbuscula from Blumea axillaris and its biological applications
T2 - ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
TI - Green synthesis of ZnO-NPs using endophytic fungal extract of Xylaria
arbuscula from Blumea axillaris and its biological applications
VL - 51
ID - 6608
ER -
TY - JOUR
AB - The objective of this study is to provide a novel synthetic approach for the
manufacture of wound-healing materials using covalently cross-linked alginate
fibers loaded with silver nanoparticles. Alginate fibers are prepared by wet-
spinning in a CaCl2 precipitation bath. Using this same approach, calcium cross-
links in alginate fibers are replaced by chemical cross-links that involve hydroxyl
groups for subsequent cross-linking by glutaraldehyde. The cross-linked fibers
become highly swollen in aqueous solution due to the presence of carboxyl
functional groups, and retain their mechanical stability in physiological fluids
owing to the stabilized network of covalent bonds. Alginate fibers can then be
loaded with silver ions via the ion-exchange reaction. Silver ions are reduced to
yield 11 nm silver nanoparticles incorporated in the polymer gel. This method
provides a convenient platform to incorporate silver nanoparticles into alginate
fibers in controlled concentrations while retaining the mechanical and swelling
properties of the alginate fibers. Our study suggests that the silver nanoparticles
loaded fibers may be easily applied in a wound healing paradigm and promote the
repair process though the promotion of fibroblast migration to the wound area,
reduction of the inflammatory phase, and the increased epidermal thickness in the
repaired wound area, thereby improving the overall quality and speed of healing. ©
2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
AU - Neibert, K.
AU - Gopishetty, V.
AU - Grigoryev, A.
AU - Tokarev, I.
AU - Al-Hajaj, N.
AU - Vorstenbosch, J.
AU - Philip, A.
AU - Minko, S.
AU - Maysinger, D.
DB - Scopus
DO - 10.1002/adhm.201200075
IS - 5
KW - Absorbable Implants
Animals
Drug Carriers
Hydrogels
Metal Nanoparticles
Mice
Mice, Nude
Silver
Skin
Treatment Outcome
Wound Healing
Wounds, Penetrating
alginic acid
calcium ion
glutaraldehyde
silver nanoparticle
animal cell
animal experiment
animal model
aqueous solution
article
biodegradability
cell migration
cell viability
controlled study
covalent bond
cross linking
cytotoxicity test
drug effect
drug formulation
drug mechanism
drug stability
fibroblast migration
hydrogel dressing
in vitro study
in vivo study
ion exchange
mouse
nonhuman
particle size
pH
precipitation
priority journal
signal transduction
wound healing
M3 - Article
PY - 2012
SP - 621-630
ST - Wound-healing with mechanically robust and biodegradable hydrogel fibers
loaded with silver nanoparticles
TI - Wound-healing with mechanically robust and biodegradable hydrogel fibers
loaded with silver nanoparticles
84879608534&doi=10.1002%2fadhm.201200075&partnerID=40&md5=cc3a3c88837110415bfda82a1
d06f2eb
VL - 1
ID - 5695
ER -
TY - JOUR
AB - PP2A, a trimeric Serine/Threonine Protein Phosphatase 2A highly expressed in
brain, is a master regulator of cellular functions. Reduction in PP2A activity has
been linked to progression of microglial mediated neuroinflammatory diseases.
Inflammatory conditions are characterized by increased population of CD86(+ve) M1
cells and a therapeutic strategy to polarize microglial cells towards CD206(+ve) M2
cells is the need of hour. In this paper we analyzed A: whether the level of PP2A
is altered in CD86(+ve) cells, B: whether FTY720, a known modulator of PP2A, is
able to restore the level of PP2A in inflamed CD86(+ve) cells. Results revealed
that PP2A activity was significantly diminished in inflamed cells but the
surprising observation was the cell viability of only 35.99% upon FTY720 treatment
in inflamed cells lacking basal PP2A activity. A sharp increase at mRNA level of
CD95 and ASK-1 indicated that apoptosis occurred in these cells through CD95/ASK-
1/JNK pathway. Importantly, flow cytometric analysis revealed apoptosis of not only
CD86(+ve) cells but also CD206(+ve) cells. Previous studies have reported that
FTY720 polarizes microglial cells towards M2 states; however apoptosis of M2 cells
was not studied. As western blot analysis revealed that FTY720 failed to completely
restore PP2A, another PP2A modulator, Memantine, was used for co-treatment. Upon
co-treatment, the level of PP2A was completely restored and also viability of
microglial cells was significantly improved with a significant reduction in
apoptosis of M2 cells. These findings suggest that co-treatment strategy may prove
beneficial to balance M1/M2 microglial population, thereby improving neuronal
functions.
AN - WOS:000599836100009
AU - Nematullah, M.
AU - Hoda, M. N.
AU - Nimker, S.
AU - Khan, F.
C7 - 115294
DA - DEC 15
DO - 10.1016/j.taap.2020.115294
PY - 2020
SN - 0041-008X
1096-0333
ST - Restoration of PP2A levels in inflamed microglial cells: Important for
neuroprotective M2 microglial viability
TI - Restoration of PP2A levels in inflamed microglial cells: Important for
neuroprotective M2 microglial viability
VL - 409
ID - 6406
ER -
TY - JOUR
AB - Silver nanoparticles are widely used in various industrial and biomedical
applications; however, little is known about their potential cardiotoxicity after
pulmonary exposure, particularly in hypertensive subjects. We assessed the
cardiotoxicity of polyethylene glycol (PEG)-coated AgNPs in hypertensive (HT) mice.
Saline (control) or PEG–AgNPs (0.5 mg/kg) were intratracheally (i.t.) instilled
four times (on days 7, 14, 21, and 28 post-angiotensin II or vehicle [saline]
infusion). On day 29, various cardiovascular parameters were evaluated. Systolic
blood pressure and heart rate were higher in PEG–AgNPs-treated HT mice than in
saline-treated HT or PEG–AgNPs-treated normotensive mice. The heart histology of
PEG–AgNPs-treated HT mice had comparatively larger cardiomyocyte damage with
fibrosis and inflammatory cells when compared with saline-treated HT mice.
Similarly, the relative heart weight and the activities of lactate dehydrogenase
and creatine kinase-MB and the concentration of brain natriuretic peptide
concentration were significantly augmented in heart homogenates of HT mice treated
with PEG–AgNPs compared with HT mice treated with saline or normotensive animals
exposed to PEG–AgNPs. Similarly, the concentrations of endothelin-1, P-selectin,
vascular cell adhesion molecule-1, and intercellular adhesion molecule-1 in heart
homogenates were significantly higher than in the other two groups when HT mice
were exposed to PEG–AgNPs. Markers of inflammation and oxidative and nitrosative
stress were significantly elevated in heart homogenates of HT mice given PEG–AgNPs
compared with HT mice treated with saline or normotensive animals exposed to PEG–
AgNPs. The hearts of HT mice exposed to PEG–AgNPs had significantly increased DNA
damage than those of HT mice treated with saline or normotensive mice treated with
AgNPs. In conclusion, the cardiac injury caused by PEG–AgNPs was aggravated in
hypertensive mice. The cardiotoxicity of PEG–AgNPs in HT mice highlights the
importance of an in-depth assessment of their toxicity before using them in
clinical settings, particularly in patients with pre-existing cardiovascular
diseases. © 2023 by the authors.
AU - Nemmar, A.
AU - Al-Salam, S.
AU - Greish, Y. E.
AU - Beegam, S.
AU - Zaaba, N. E.
AU - Ali, B. H.
C7 - 8890
DB - Scopus
DO - 10.3390/ijms24108890
IS - 10
KW - cardiotoxicity
coating
hypertension
polyethylene glycol
Animals
Blood Pressure
Cardiotoxicity
Hypertension
Metal Nanoparticles
Mice
Silver
macrogol
metal nanoparticle
silver
animal
blood pressure
mouse
M3 - Article
PY - 2023
ST - Impact of Intratracheal Administration of Polyethylene Glycol-Coated Silver
Nanoparticles on the Heart of Normotensive and Hypertensive Mice
TI - Impact of Intratracheal Administration of Polyethylene Glycol-Coated Silver
Nanoparticles on the Heart of Normotensive and Hypertensive Mice
85160380376&doi=10.3390%2fijms24108890&partnerID=40&md5=85acdd68c80d0413a07038a5729
acbb0
VL - 24
ID - 4983
ER -
TY - JOUR
AB - Wound dressings with silver have been shown to be cytotoxic in vitro.
However, the extrapolation of this cytotoxicity to clinical settings is unclear. We
applied dressings with various forms of silver on porcine skin ex vivo and
investigated silver penetration and DNA damage. We assessed antimicrobial efficacy,
cytotoxicity to skin cells, and immune response induced by the dressings. All
dressings elevated the DNA damage marker γ-H2AX and the expression of stress-
related genes in explanted skin relative to control. This corresponded with the
amount of silver in the skin. The dressings reduced viability, induced oxidative
stress and DNA damage in skin cells, and induced the production of pro-inflammatory
IL-6 by monocytes. The oxidative burst and viability of activated neutrophils
decreased. The amount of silver released into the culture medium varied among the
dressings and correlated with in vitro toxicity. However, antimicrobial
efficiencies did not correlate strongly with the amount of silver released from the
dressings. Antimicrobial efficiency and toxicity are driven by the form of silver
and the construction of dressings and not only by the silver concentration. The
damaging effects of silver dressings in ex vivo skin highlight the importance of
thorough in vivo investigation of silver dressing toxicity. © 2020, The Author(s).
AU - Nešporová, K.
AU - Pavlík, V.
AU - Šafránková, B.
AU - Vágnerová, H.
AU - Odráška, P.
AU - Žídek, O.
AU - Císařová, N.
AU - Skoroplyas, S.
AU - Kubala, L.
AU - Velebný, V.
C7 - 15216
DB - Scopus
DO - 10.1038/s41598-020-72249-3
IS - 1
KW - Animals
Bandages
Cell Line
Cell Survival
DNA Damage
Humans
Silver
Skin
Swine
Tissue Culture Techniques
Wound Infection
silver
adverse device effect
animal
bandage
cell line
cell survival
chemistry
cytology
DNA damage
drug effect
human
pig
skin
tissue culture technique
wound infection
M3 - Article
PY - 2020
ST - Effects of wound dressings containing silver on skin and immune cells
TI - Effects of wound dressings containing silver on skin and immune cells
85091128840&doi=10.1038%2fs41598-020-72249-
3&partnerID=40&md5=c50bb47dbebe3995cff618a01c1fcc2d
VL - 10
ID - 5317
ER -
TY - JOUR
AB - Wound dressings with silver have been shown to be cytotoxic in vitro.
However, the extrapolation of this cytotoxicity to clinical settings is unclear. We
applied dressings with various forms of silver on porcine skin ex vivo and
investigated silver penetration and DNA damage. We assessed antimicrobial efficacy,
cytotoxicity to skin cells, and immune response induced by the dressings. All
dressings elevated the DNA damage marker gamma -H(2)AX and the expression of
stress-related genes in explanted skin relative to control. This corresponded with
the amount of silver in the skin. The dressings reduced viability, induced
oxidative stress and DNA damage in skin cells, and induced the production of pro-
inflammatory IL-6 by monocytes. The oxidative burst and viability of activated
neutrophils decreased. The amount of silver released into the culture medium varied
among the dressings and correlated with in vitro toxicity. However, antimicrobial
efficiencies did not correlate strongly with the amount of silver released from the
dressings. Antimicrobial efficiency and toxicity are driven by the form of silver
and the construction of dressings and not only by the silver concentration. The
damaging effects of silver dressings in ex vivo skin highlight the importance of
thorough in vivo investigation of silver dressing toxicity.
AN - WOS:000573768800024
AU - Nesporova, K.
AU - Pavlik, V.
AU - Safrankova, B.
AU - Vagnerova, H.
AU - Odraska, P.
AU - Zidek, O.
AU - Cisarova, N.
AU - Skoroplyas, S.
AU - Kubala, L.
AU - Velebny, V.
C7 - 15216
DA - SEP 16
DO - 10.1038/s41598-020-72249-3
IS - 1
PY - 2020
SN - 2045-2322
ST - Effects of wound dressings containing silver on skin and immune cells
TI - Effects of wound dressings containing silver on skin and immune cells
VL - 10
ID - 6233
ER -
TY - JOUR
AB - Chronic wounds have emerged as a major cause of mortality, especially in
patients with diabetes and other pathologies. Statistics indicate that chronic
wounds affect around 6.5 million patients annually, with wound care and management
incurring huge economic costs. Growing incidence of chronic wounds and associated
pathologies along with the limitations of current therapies have established a
strong need for novel and innovative approaches to accelerate wound healing.
Conventionally, chronic wounds are addressed using various FDA-approved silver-
based formulations and other biomaterials. However, the toxicity associated with
these conventional approaches, along with the increased frequency of chronic wound
cases, makes the development of alternative therapies for effective wound healing
necessary. Recently, researchers have investigated the design and development of
nanoparticles, especially inorganic metal nanoparticles, as promising candidates
for addressing various pathological conditions, including wound healing. Several
research groups, including ours, have designed numerous metal nanoparticles
(including silver, gold, zinc oxide, cerium oxide, terbium hydroxide, silica,
titanium oxide, copper) and demonstrated their wound-healing properties using in
vitro and in vivo models. The rise of nanotechnology-based platforms in wound
healing is evidenced by the tremendous impact and number of publications observed
in recent years, which has emphasized the robust potential of inorganic
nanomedicine for addressing wounds. Therefore, the importance of these inorganic
nanomaterial-based interventions for wound-healing applications needs to be
emphasized to inform and encourage scientists and young researchers globally to
engage with this expanding area of biology and medicine. In this review article, we
mainly focus on highlighting the role of inorganic nanomaterials and nanomaterial-
based approaches for wound healing and tissue regeneration, along with their
mechanistic properties, clinical status, challenges, and future directions. © The
AU - Nethi, S. K.
AU - Das, S.
AU - Patra, C. R.
AU - Mukherjee, S.
DB - Scopus
DO - 10.1039/c9bm00423h
IS - 7
KW - Animals
Humans
Nanostructures
Nanotechnology
Signal Transduction
Wound Healing
Cerium oxide
Gold compounds
Pathology
Silica
Terbium compounds
Tissue regeneration
Titanium oxides
Zinc oxide
antiinfective agent
copper nanoparticle
glucocorticoid
gold nanoparticle
graphene oxide
nanomaterial
nanoparticle
nonmetallic inorganic nanoparticle
silica nanoparticle
silver nanoparticle
super paramagnetic iron oxide nanoparticle
terbium hydroxide nanoparticle
unclassified drug
biomaterial
Biology and medicine
Conventional approach
Design and Development
Innovative approaches
Inorganic nanomaterials
Mechanistic properties
Pathological conditions
angiogenesis
cell engineering
heart surgery
hemostasis
hemostasis phase
human
hyperbaric oxygen therapy
inflammatory phase
maturation phase
nanomedicine
nanotechnology
nerve regeneration
nonhuman
priority journal
proliferative phase
Review
tissue regeneration
vascular surgery
wound healing
animal
chemistry
drug effect
procedures
signal transduction
Metal nanoparticles
M3 - Review
PY - 2019
SP - 2652-2674
ST - Recent advances in inorganic nanomaterials for wound-healing applications
TI - Recent advances in inorganic nanomaterials for wound-healing applications
85067938096&doi=10.1039%2fc9bm00423h&partnerID=40&md5=e49f8d782dc1604dffe46cab695d1
040
VL - 7
ID - 5360
ER -
TY - JOUR
AB - The use of selective barriers as resorbable membranes has become a routine
clinical procedure for guided bone regeneration. Therefore, the production of
membranes with a low inflammatory potential during their resorption process has
become the goal of a considerable number of researches. Aim: The purpose of the
present study was to evaluate the biocompatibility of poly (L-lactic acid) (PLLA)
and biocelulose membranes (BC) inserted in the subcutaneous tissue on the dorsum of
rats. Methods: Fifteen animals underwent surgical procedures for the insertion of 4
types of membranes: COL (Collagen membrane) – Control Group; BC (Biocellulose
membrane); BCAg (Biocellulose membrane impregnated with Silver); PLLA (Poly (L-
lactic acid) membrane). All membrane types were inserted into each animal. Animals
were euthanized after 3, 7, and 15 days of the surgical procedure. Descriptive
histological analyses were carried out to investigate host tissue reaction to
membrane presence by assessing the anti-inflammatory process composition associated
with the membrane resorption and the presence of foreign-body reaction or
encapsulation. Results: The BC membranes showed a higher degree of inflammation and
poor pattern of integration with the surrounding tissues than the PLLA and COL
membranes. Conclusion: The PLLA and COL membranes present better biocompatibility
than the BC membranes. © 2022,Brazilian Journal of Oral Sciences. All Rights
Reserved.
AU - Neto, J. D.
AU - Marques, R. F. C.
AU - Motta, A. C.
AU - de Rezende Duek, E. A.
AU - de Oliveira, G. J. P. L.
AU - Marcantonio, C.
C7 - e220616
DB - Scopus
DO - 10.20396/BJOS.V21I00.8670616
Celulose
Inflammation
Membranes
Polyesters
M3 - Article
PY - 2021
ST - Analysis of the biocompatibility of a biocelulose and a poly L-lactic acid
membrane
T2 - Brazilian Journal of Oral Sciences
TI - Analysis of the biocompatibility of a biocelulose and a poly L-lactic acid
membrane
85138663425&doi=10.20396%2fBJOS.V21I00.8670616&partnerID=40&md5=98d758a6be3f96752c6
a68f0cc534835
VL - 21
ID - 5162
ER -
TY - JOUR
AB - Cerium oxide nanoparticle (CeO-NP) was synthesized using Origanum majorana L.
leaf extract and characterized using particle size analyzer, transmission electron
microscopy (TEM), field emission scanning electron microscopy (FESEM), X-ray
diffraction (XRD) and Fourier transform infrared (FTIR). The antioxidant properties
and cytotoxic effects of CeO-NP in human breast carcinoma cells (MDA-MB-231 cell
line) and human umbilical vein endothelial cells (HUVEC) as normal cells were
evaluated. To determine the probable molecular mechanism of action of CeO-NP on
cellular redox and anti-inflammatory potential, the expressions of antioxidant-
related genes catalase (CAT), superoxide dismutase (SOD) in HUVEC cell line were
also analyzed. The results indicated that spherically shaped nanoparticles with a
size of 10-70 nm bound to functional phenolic and flavonoids from O. majorana L.
leaf extract. The green synthesized CeO-NP showed antioxidant activity by free
radical scavenging activity against DPPH and ABTS free radicals. The antioxidant
activity was significantly (p < 0.001) lower than that of Butylated hydroxyanisole
(BHA) as a reference antioxidant. The obtained results elucidated that CeO-NP
possessed cytotoxicity. The cytotoxic effects of CeO-NP were higher against MDA-MB-
231 cancer cells compared to HUVEC normal cells. In addition, this NP was capable
to enhance the expression of CAT and SOD as main antioxidant-related genes.
Consequently, the higher cytotoxic effects of CeO-NP against breast cancer compared
to normal cells indicated the potential use of this NP as anti-cancer agent.
However, more research on its cytotoxicity against other cancer cells and
mechanisms in which this NP exert its anti-cancer properties should be performed.
AN - WOS:000509524100013
AU - Nezhad, S. A.
AU - Es-haghi, A.
AU - Tabrizi, M. H.
C7 - e5314
DA - FEB
DO - 10.1002/aoc.5314
IS - 2
PY - 2020
SN - 0268-2605
1099-0739
ST - Green synthesis of cerium oxide nanoparticle using Origanum majorana L. leaf
extract, its characterization and biological activities
T2 - APPLIED ORGANOMETALLIC CHEMISTRY
TI - Green synthesis of cerium oxide nanoparticle using Origanum majorana L. leaf
extract, its characterization and biological activities
VL - 34
ID - 6562
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) are highly relevant for human and environmental
exposure due to their widespread use in consumer and medical products and various
applications. Thus, there is a need for evaluating potential toxicity of these NPs.
The objective of this study was to investigate the toxic effects of the OECD
(Organization for Economic Co-operation and Development) representative Ag-NPs,
NM300K, in mouse macrophage J774A.1 and human colonic epithelial HT29 cells, using
multiple endpoint assays. Exposure of test cells to different concentrations (1-250
μg/mL; total silver content) of NM300K for 24 h showed a dose-dependent decrease in
cell viability. At high doses, NM300K altered cell shape and induced the formation
of vacuolar structures, as examined by confocal and electron microscopy. Moreover,
NM300K induced inflammation as evidenced by the elevated levels of pro-inflammatory
cytokines. Finally, high doses of NM300K led to increased production of reactive
oxygen species and induction of oxidative stress, leading to oxidative DNA damage
and apoptosis in test cells. At equivalent silver concentrations, NM300K were less
cytotoxic than AgNO3. However, the similar patterns in the effects of NM300K and
AgNO3 throughout the assessed toxicological endpoints suggest that Ag+ released
from these NPs by dissolution could be a primary contributor to toxicity. This
study is among the first to characterize the potential toxicity of OECD
representative AgNPs in vitro, and provides additional insight into the biological
mechanisms associated with Ag-NP toxicity. © 2016.
AU - Nguyen, K. C.
AU - Richards, L.
AU - Massarsky, A.
AU - Moon, T. W.
AU - Tayabali, A. F.
DB - Scopus
DO - 10.1016/j.tiv.2016.03.004
KW - Apoptosis
DNA damage
Epithelial
Macrophage
NM300K
Oxidative stress
Toxicity
Animals
Caspase 3
Cell Line
Cell Survival
DNA Damage
Epithelial Cells
Glutathione
HT29 Cells
Humans
Macrophages
Metal Nanoparticles
Mice
Oxidative Stress
Silver
caspase 3
DNA
eotaxin
glutathione
interleukin 12p70
interleukin 1beta
interleukin 4
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
protein bcl 2
silver nanoparticle
silver nitrate
metal nanoparticle
silver
animal cell
apoptosis
Article
cell shape
cell vacuole
cell viability
controlled study
cytokine release
cytotoxicity
enzyme activity
human
human cell
in vitro study
inflammation
intestine epithelium cell
macrophage
mouse
nonhuman
oxidative stress
particle size
animal
cell line
cell survival
drug effects
epithelium cell
HT-29 cell line
metabolism
M3 - Article
PY - 2016
SP - 163-173
ST - Toxicological evaluation of representative silver nanoparticles in
macrophages and epithelial cells
TI - Toxicological evaluation of representative silver nanoparticles in
macrophages and epithelial cells
84961813433&doi=10.1016%2fj.tiv.2016.03.004&partnerID=40&md5=d677272be5cf1b783dc610
7662381d21
VL - 33
ID - 5530
ER -
TY - CONF
AB - This study compares toxic effects of uncoated (20, 40, 60 and 80 nm) and OECD
(Organization for Economic Co-operation and Development) standard citrate- and
polyvinylpyrrolidone (PVP)-coated (10, 50, and 75 nm) silver nanoparticles (Ag-NPs)
in J774A. 1 macrophage and HT29 epithelial cells. The cells were exposed to
different concentrations (silver content) of Ag-NPs for 24 h. Analysis showed that
uncoated Ag-NPs, at a concentration of 1 μg/ml, decreased cell viability by 20-40%
and that 20 and 40 nm particles were 10% more cytotoxic than the 60 and 80 nm
particles. In exposures to coated Ag-NPs, cell viability dropped at 25 μg/ml or
higher concentrations, and the effects were also size-dependent. PVP-coated
particles induced greater cytotoxicity than citrate-coated particles. Changes in
sub-cellular architecture were observed in J774A. 1 cells upon exposure to test Ag-
NPs. Furthermore, uncoated Ag-NPs (1 μg/mL) decreased the expression of selected
cytokines including TNF-α, IL-1β, and IL-12 (p70) in J774A. 1 and IL-8 in HT29
cells. In contrast, both citrate- and PVP-coated Ag-NPs increased the expression of
these cytokines at higher concentrations (25 μg/ml), and PVP-coated particles
elevated cytokine levels the most. Moreover, while uncoated Ag-NPs resulted in
decreased glutathione (GSH) content and increased superoxide dismutase (SOD)
activity in test cells in a size-dependent manner at 1 μg/ml, coated Ag-NPs caused
non-significant changes in GSH and SOD, even at the highest test concentrations.
Lastly, uncoated (20 and 40 nm) at 1 μg/ml and coated Ag-NPs (10 nm PVP) at 50
μg/ml slightly increased the production of reactive oxygen species (ROS). Our data
showed that uncoated Ag-NPs are more toxic than coated Ag-NPs. While uncoated Ag-
NPs appear to suppress inflammatory responses and enhance oxidative stress in the
test cells, coated Ag-NPs induce toxic effects through up-regulation of cytokines.
Our findings support the toxicity of Ag-NPs as being size- and coating- dependent
while providing additional insight on the health impact of Ag-NPs. © IOP Publishing
Ltd 2013.
AU - Nguyen, K. C.
AU - Seligy, V. L.
AU - Massarsky, A.
AU - Moon, T. W.
AU - Rippstein, P.
AU - Tan, J.
DB - Scopus
DO - 10.1088/1742-6596/429/1/012025
ET - 1
KW - Cells
Cytology
Cytotoxicity
Enzymes
Industrial poisons
International cooperation
Metal nanoparticles
Nanoparticles
Oxygen
Toxicity
Epithelial cells
Organization for economic co-operation and development
Polyvinyl pyrrolidone
Superoxide dismutase activities
Silver
PY - 2013
ST - Comparison of toxicity of uncoated and coated silver nanoparticles
T2 - Journal of Physics: Conference Series
TI - Comparison of toxicity of uncoated and coated silver nanoparticles
84876858000&doi=10.1088%2f1742-
6596%2f429%2f1%2f012025&partnerID=40&md5=9ca06fab852f23e0ba45483634b5870c
VL - 429
ID - 5715
ER -
TY - CPAPER
AB - This study compares toxic effects of uncoated (20, 40, 60 and 80 nm) and OECD
(Organization for Economic Co-operation and Development) standard citrate-and
polyvinylpyrrolidone (PVP)-coated (10, 50, and 75 nm) silver nanoparticles (Ag-NPs)
in J774A.1 macrophage and HT29 epithelial cells. The cells were exposed to
different concentrations (silver content) of Ag-NPs for 24 h. Analysis showed that
uncoated Ag-NPs, at a concentration of 1 mu g/ml, decreased cell viability by 20-
40% and that 20 and 40 nm particles were 10% more cytotoxic than the 60 and 80 nm
particles. In exposures to coated Ag-NPs, cell viability dropped at 25 mu g/ml or
higher concentrations, and the effects were also size-dependent. PVP-coated
particles induced greater cytotoxicity than citrate-coated particles. Changes in
sub-cellular architecture were observed in J774A. 1 cells upon exposure to test Ag-
NPs. Furthermore, uncoated Ag-NPs (1 mu g/mL) decreased the expression of selected
cytokines including TNF-alpha, IL-1 beta, and IL-12 (p70) in J774A. 1 and IL-8 in
HT29 cells. In contrast, both citrate-and PVP-coated Ag-NPs increased the
expression of these cytokines at higher concentrations (25 mu g/ml), and PVP-coated
particles elevated cytokine levels the most. Moreover, while uncoated Ag-NPs
resulted in decreased glutathione (GSH) content and increased superoxide dismutase
(SOD) activity in test cells in a size-dependent manner at 1 mu g/ml, coated Ag-NPs
caused non-significant changes in GSH and SOD, even at the highest test
concentrations. Lastly, uncoated (20 and 40 nm) at 1 mu g/ml and coated Ag-NPs (10
nm PVP) at 50 mu g/ml slightly increased the production of reactive oxygen species
(ROS). Our data showed that uncoated Ag-NPs are more toxic than coated Ag-NPs.
While uncoated Ag-NPs appear to suppress inflammatory responses and enhance
oxidative stress in the test cells, coated Ag-NPs induce toxic effects through up-
regulation of cytokines. Our findings support the toxicity of Ag-NPs as being size-
and coating-dependent while providing additional insight on the health impact of
Ag-NPs.
AN - WOS:000318429400025
AU - Nguyen, K. C.
AU - Seligy, V. L.
AU - Massarsky, A.
AU - Moon, T. W.
AU - Rippstein, P.
AU - Tan, J.
AU - Iop
DO - 10.1088/1742-6596/429/1/012025
PY - 2013
T2 - NANOSAFE 2012: INTERNATIONAL CONFERENCES ON SAFE PRODUCTION AND USE OF
NANOMATERIALS
TI - Comparison of toxicity of uncoated and coated silver nanoparticles
VL - 429
ID - 6003
ER -
TY - JOUR
AB - Since about 70% of commercial biopharmaceutical products have been produced
in Chinese hamster ovary (CHO) cells, this cell line is undeniably a workhorse for
biopharmaceuticals production. Meanwhile, sialic acid terminals were reported to
affect anti-inflammatory activity, antibody-dependent cellular cytotoxicity
efficacy of IgG antibodies. Taking these findings together, we aimed to establish
CHO cell lines that highly produce sialic acid terminals by overexpressing two N-
acetylneuraminic acid-based key enzymes, α(2,6)-sialyltransferase and UDP-N-
acetylglucosamine 2-epimerase/N-acetylmannosamine kinase using dihydrofolate
reductase/methotrexate gene amplification method. Indeed, the number of total
sialic acid terminal glycan structures increased tremendously, by 12-fold compared
to the wild type in total protein extracts. With the methotrexate supplementation,
a targeted cell line, CHOmt17-100, showed up to 1.4 times more sialylated
structures of glycoforms in total proteins. Interestingly, immunoglobulin G, used
as the model protein in CHOmt17-100, showed about 53% sialylated structures in its
glycoforms. These resultant sialylated glycans exhibited more than approximately
14.5 times increase as compared to that of the wild type. Moreover, the resultant
glycan structures mostly had N-acetylneuraminic acid terminals, while N-
glycolylneuraminic acid terminal composition remained less than 5% as compared to
the wild type. Engineered antibodies derived from CHO cell lines that produce high
levels of sialic acid will contribute to the examination of glycoforms’ efficacy
and usefulness toward bio-better products. © 2020, Springer Nature B.V.
AU - Nguyen, T. S.
AU - Misaki, R.
AU - Ohashi, T.
AU - Fujiyama, K.
DB - Scopus
DO - 10.1007/s10616-020-00381-z
IS - 3
KW - Antibody
Chinese hamster ovary (CHO) cell
Glycosylation
IgG
Sialyltransferase
UDP-GlcNAc 2-epimerase/ManNAc kinase
dihydrofolate reductase
glycan
immunoglobulin G
methotrexate
n acetylman nosamine kinase
n acetylneuraminic acid
phosphotransferase
recombinant antibody
sialic acid
sialyltransferase
unclassified drug
uridine diphosphate n acetylglucosamine 2 epimerase
animal cell
antibody dependent cellular cytotoxicity
Article
cell culture
cell density
cell viability
CHO cell line
comparative study
controlled study
female
gene amplification
internal ribosome entry site
methotrexate-resistant cell line
nonhuman
protein glycosylation
sialylation
silver staining
M3 - Article
PY - 2020
SP - 343-355
ST - Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary
cells
T2 - Cytotechnology
TI - Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary
cells
85080972492&doi=10.1007%2fs10616-020-00381-
z&partnerID=40&md5=044b5a2ad715309579f1b0ee77c9368e
VL - 72
ID - 5261
ER -
TY - JOUR
AB - The airway epithelium is critical for maintaining innate and adaptive immune
responses, and occupational exposures that disrupt its immune homeostasis may
initiate and amplify airway inflammation. In our previous study, we demonstrated
that silver nanoparticles (AgNP), which are engineered nanomaterials used in
multiple applications but primarily in the manufacturing of many antimicrobial
products, induce toxicity in organotypic cultures derived from murine tracheal
epithelial cells (MTEC), and those differentiated toward a "Type 2 [T2]-Skewed"
phenotype experienced an increased sensitivity to AgNP toxicity, suggesting that
asthmatics could be a sensitive population to AgNP exposures in occupational
settings. However, the mechanistic basis for this genotype x phenotype (GxP)
interaction has yet to be defined. In this study, we conducted transcriptional
profiling using RNA-sequencing to predict the enrichment of specific canonical
pathways and upstream transcriptional regulators to assist in defining a
mechanistic basis for GxP effects on AgNP toxicity. Organotypic cultures were
derived from MTEC across 2 genetically inbred mouse strains (A/J and C57BL/6J
mice), 2 phenotypes ("Normal" and "T2-Skewed"), and 1 AgNP exposure (an acute 24 h
exposure) to characterize GxP effects on transcriptional response to AgNP toxicity.
The "T2-Skewed" phenotype was marked by increased pro-inflammatory T17 responses to
AgNP toxicity, which are significant predictors of neutrophilic/difficult-to-
control asthma and suggests that asthmatics could be a sensitive population to AgNP
exposures in occupational settings. This study highlights the importance of
considering GxP effects when identifying these sensitive populations, whose
underlying genetics or diseases could directly modify their response to AgNP
exposures.
AN - WOS:000508116400013
AU - Nicholas, T. P.
AU - Haick, A. K.
AU - Bammler, T. K.
AU - Workman, T. W.
AU - Kavanagh, T. J.
AU - Gharib, S. A.
AU - Altemeier, W. A.
DA - JAN
DO - 10.1093/toxsci/kfz209
IS - 1
PY - 2020
SN - 1096-6080
1096-0929
SP - 131-143
ST - The Effects of Genotype x Phenotype Interactions on Transcriptional Response
to Silver Nanoparticle Toxicity in Organotypic Cultures of Murine Tracheal
Epithelial Cells
TI - The Effects of Genotype x Phenotype Interactions on Transcriptional Response
Epithelial Cells
VL - 173
ID - 6082
ER -
TY - JOUR
AB - The airway epithelium is critical for maintaining innate and adaptive immune
responses, and occupational exposures that disrupt its immune homeostasis may
initiate and amplify airway inflammation. In our previous study, we demonstrated
that silver nanoparticles (AgNP), which are engineered nanomaterials used in
multiple applications but primarily in the manufacturing of many antimicrobial
products, induce toxicity in organotypic cultures derived from murine tracheal
epithelial cells (MTEC), and those differentiated toward a "Type 2 [T2]-Skewed"
phenotype experienced an increased sensitivity to AgNP toxicity, suggesting that
asthmatics could be a sensitive population to AgNP exposures in occupational
settings. However, the mechanistic basis for this genotype × phenotype (G × P)
interaction has yet to be defined. In this study, we conducted transcriptional
profiling using RNA-sequencing to predict the enrichment of specific canonical
pathways and upstream transcriptional regulators to assist in defining a
mechanistic basis for G × P effects on AgNP toxicity. Organotypic cultures were
derived from MTEC across 2 genetically inbred mouse strains (A/J and C57BL/6J
mice), 2 phenotypes ("Normal" and "T2-Skewed"), and 1 AgNP exposure (an acute 24 h
exposure) to characterize G × P effects on transcriptional response to AgNP
toxicity. The "T2-Skewed" phenotype was marked by increased pro-inflammatory T17
responses to AgNP toxicity, which are significant predictors of
neutrophilic/difficult-to-control asthma and suggests that asthmatics could be a
sensitive population to AgNP exposures in occupational settings. This study
highlights the importance of considering G × P effects when identifying these
sensitive populations, whose underlying genetics or diseases could directly modify
their response to AgNP exposures. © 2019 The Author(s). All rights reserved.
AU - Nicholas, T. P.
AU - Haick, A. K.
AU - Bammler, T. K.
AU - Workman, T. W.
AU - Kavanagh, T. J.
AU - Gharib, S. A.
AU - Altemeier, W. A.
DB - Scopus
DO - 10.1093/toxsci/kfz209
IS - 1
KW - airway epithelial cells
gene-environment interactions
inflammation
organotypic cultures
Animals
Cell Count
Epithelial Cells
Epithelium
Genotype
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Phenotype
Respiratory System
Silver
Toxicity Tests
complementary DNA
CXCL1 chemokine
CXCL2 chemokine
epithelial derived neutrophil activating factor 78
interleukin 10
interleukin 13
interleukin 25
interleukin 33
interleukin 5
interleukin 6
interleukin 9
RNA 28S
silver nanoparticle
STAT3 protein
thymic stromal lymphopoietin
antiinfective agent
metal nanoparticle
silver
animal experiment
animal model
Article
asthma
cell culture
controlled study
gene expression
gene interaction
genetic analysis
genetic variability
genotype environment interaction
in vitro study
inbred mouse strain
mouse
nanotoxicology
neutrophil
nonhuman
phenotypic variation
respiration control
RNA sequencing
trachea epithelium
transcription regulation
animal
C57BL mouse
cell count
drug effect
epithelium
epithelium cell
genotype
phenotype
respiratory system
toxicity testing
M3 - Article
PY - 2020
SP - 131-143
ST - The Effects of Genotype × Phenotype Interactions on Transcriptional Response
Epithelial Cells
TI - The Effects of Genotype × Phenotype Interactions on Transcriptional Response
Epithelial Cells
85077488162&doi=10.1093%2ftoxsci
%2fkfz209&partnerID=40&md5=82c41f2313bb60abe407adedc00fbf22
VL - 173
ID - 5364
ER -
TY - JOUR
AB - The miR-29 family is involved in fibrosis in multiple organs, including the
intestine where miR-29b facilitates TGF-beta-mediated up-regulation of collagen in
mucosal fibroblasts from Crohn's disease (CD) patients. Myeloid cell leukemia-1
(MCL-1), a member of the B-cell CLL/Lymphoma 2 (BCL-2) apoptosis family, is
involved in liver fibrosis and is targeted by miR-29b via its 3'-UTR in cultured
cell lines. We investigate the role of MCL-1 and miR-29b in primary intestinal
fibroblasts and tissue from stricturing CD patients. Transfection of CD intestinal
fibroblasts with pre-miR-29b resulted in a significant increase in the mRNA
expression of MCL-1 isoforms [MCL-1Long (L)/Extra Short (ES) and MCL-1Short (S)],
although MCL-1S was expressed at significantly lower levels. Western blotting
predominantly detected the anti-apoptotic MCL-1L isoform, and immunofluorescence
showed that staining was localised in discrete nuclear foci. Transfection with pre-
miR-29b or anti-miR-29b resulted in a significant increase or decrease,
respectively, in MCL-1L foci. CD fibroblasts treated with IL-6 and IL-8,
inflammatory cytokines upstream of MCL-1, increased the total mass of MCL-1L-
positive foci. Furthermore, transfection of intestinal fibroblasts with pre-miR-29b
resulted in an increase in mRNA and protein levels of IL-6 and IL-8. Finally,
immunohistochemistry showed reduced MCL-1 protein expression in fibrotic CD samples
compared to non-stricturing controls. Together, our findings suggest that induction
of MCL-1 by IL-6/IL-8 may surmount any direct down-regulation by miR-29b via its
3'-UTR. We propose that an anti-fibrotic miR-29b/IL-6 IL-8/MCL-1L axis may
influence intestinal fibrosis in CD. In the future, therapeutic modulation of this
pathway might contribute to the management of fibrosis in CD.
AN - WOS:000399916800009
AU - Nijhuis, A.
AU - Curciarello, R.
AU - Mehta, S.
AU - Feakins, R.
AU - Bishop, C. L.
AU - Lindsay, J. O.
AU - Silver, A.
DA - MAY
DO - 10.1007/s00441-017-2576-1
IS - 2
PY - 2017
SN - 0302-766X
1432-0878
SP - 325-335
ST - MCL-1 is modulated in Crohn's disease fibrosis by miR-29b via IL-6 and IL-8
T2 - CELL AND TISSUE RESEARCH
TI - MCL-1 is modulated in Crohn's disease fibrosis by miR-29b via IL-6 and IL-8
VL - 368
ID - 6689
ER -
TY - JOUR
AB - 3D printing provides numerous opportunities for designing tissue engineering
constructs with intricate porosity, geometry and favourable mechanical properties
and has the potential to revolutionize medical treatments. However, an often-
encountered restriction is the selection of materials suitable for utilization in
3D printing, not all of which have appropriate biocompatibility properties. In this
work, fused deposition modeling was employed to fabricate 3D PCL constructs without
the use of any solvent. Plasma deposition was used to modify the surface of the
scaffolds, followed by immobilization of silver nanoparticles. The physico-chemical
and mechanical analyses demonstrated that the scaffolds retained their porosity and
mechanical integrity. The mechanical properties evaluated by the nanoindentation
technique demonstrated an increase in reduced modulus to 1.87 ± 0.012 GPa for PCL
scaffolds functionalized with silver nanoparticles for 24 hours. We also showed
complete prevention of colonization by medically relevant pathogens. The modified
scaffolds had good biocompatibility. The immune response studies in the culture of
macrophages confirmed a reduction in the level of expression of pro-inflammatory
cytokines which is a key requirement for successful wound healing. The in vivo
studies on Sprague Dawley rats indicated enhanced angiogenesis and the absence of
foreign body reaction for scaffolds functionalized with silver nanoparticles for 6
hours. The 3D printing approach presented in this study provides new sustainable
opportunities that can be adopted for designing biomaterial constructs with
enhanced biological properties. © 2021 The Royal Society of Chemistry.
AU - Ninan, N.
AU - Joseph, B.
AU - Visalakshan, R. M.
AU - Bright, R.
AU - Denoual, C.
AU - Zilm, P.
AU - Dalvi, Y. B.
AU - Priya, P. V.
AU - Mathew, A.
AU - Grohens, Y.
AU - Kalarikkal, N.
AU - Vasilev, K.
AU - Thomas, S.
DB - Scopus
DO - 10.1039/d1ma00444a
IS - 20
M3 - Article
PY - 2021
SP - 6620-6630
ST - Plasma assisted design of biocompatible 3D printed PCL/silver nanoparticle
scaffolds: In vitro and in vivo analyses
T2 - Materials Advances
TI - Plasma assisted design of biocompatible 3D printed PCL/silver nanoparticle
scaffolds: In vitro and in vivo analyses
85117684440&doi=10.1039%2fd1ma00444a&partnerID=40&md5=d507c6d0b3d616e2c25ae85584919
55d
VL - 2
ID - 5197
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) are gaining a lot of attention in recent decades
from researchers due to their unique optoelectronic properties and their
significance in the field of biomedicine. Keeping this in view, our research work
was designed to investigate gold nanoparticles obtained by using a fungal
endophytic strain Chaetomium globosum, isolated from Vitex negundo which showed
significant activity on enzyme inhibition. In the present study, the fungal isolate
C. globosum was characterized using HPLC and LC-MS. A novel compound Catechin was
matched with standard Catechin. Further, the endophyte C. globosum extract was
utilized to synthesize gold nanoparticles (CgAuNPs) which was analysed by UV-
visible spectroscopy. The CgAuNPs exhibited wine red color and the absorption peak
appeared at 542 nm confirming the formation of the AuNPs. Further, Fourier
Transmission Infrared Spectroscopy (FTIR) was performed to confirm the various
functional groups present in mycosynthesized CgAuNPs. FTIR analysis demonstrated
the presence of amines, flavonoids, as well as the presence of amide I linkage
which possibly reduces Au+ to Au-0. The synthesized CgAuNPs exhibited potential
cytotoxicity against HeLa cells in a dose dependent manner. Further, CgAuNPs
demonstrated significant antiinflammatory activity. Overall, the present work
provides insights into the design of nano delivery and may be applied for clinical
studies in future.
AN - WOS:000597176900011
AU - Ningaraju, S.
AU - Munawer, U.
AU - Raghavendra, V. B.
AU - Balaji, K. S.
AU - Melappa, G.
AU - Brindhadevi, K.
AU - Pugazhendhi, A.
C7 - 113970
DA - JAN 1
DO - 10.1016/j.ab.2020.113970
PY - 2021
SN - 0003-2697
1096-0309
ST - Chaetomium globosum extract mediated gold nanoparticle synthesis and potent
anti-inflammatory activity
T2 - ANALYTICAL BIOCHEMISTRY
TI - Chaetomium globosum extract mediated gold nanoparticle synthesis and potent
anti-inflammatory activity
VL - 612
ID - 6314
ER -
TY - JOUR
AB - Recent advances in particle-forming chemistries used for developing
nanotechnology has not only widened novel applications for nanoscale materials but
also has provided significant concern regarding their biological effects. The
present study investigates the inflammatory responses of RAW 264.7 mouse
macrophages exposed to nanoparticles (NPs, 5 mu g/ml) of varied sizes including
silver (Ag), aluminum (Al), carbon black (CB), carbon-coated silver (CAg) and gold
(Au). A significant increase in IL-6, reactive oxygen species (ROS) generation,
nuclear translocation of nuclear factor-kappa B (NF-kappa B), induction of
cyclooxygenase-2 (COX-2), and tumor necrosis factor-alpha (TNF-alpha) expression
was observed in macrophages with maximum response found in cells exposed to Ag NPs
followed by Al, CB and CAg. These pro-inflammatory effects of NPs were dependent on
size and duration of exposure and comparable to those induced by lipopolysaccharide
(LPS), a known inflammatory mediator. Au NPs, on the other hand, induced small but
significant inflammatory responses in macrophages upon prolonged exposure. These
studies reveal that Ag NPs exhibit higher propensity in inducing inflammation,
mediated by ROS and NF-kappa B signaling pathways and leading to the induction of
COX-2, TNF-alpha and IL-6. However, no such prominent pro-inflammatory responses
were observed with Au NPs, suggesting their bio-compatibility.
AN - WOS:000296633100005
AU - Nishanth, R. P.
AU - Jyotsna, R. G.
AU - Hussain, S. M.
AU - Reddanna, P.
DA - DEC
DO - 10.3109/17435390.2010.541604
IS - 4
PY - 2011
SN - 1743-5390
1743-5404
SP - 502-516
ST - Inflammatory responses of RAW 264.7 macrophages upon exposure to
nanoparticles: Role of ROS-NF kappa B signaling pathway
T2 - NANOTOXICOLOGY
TI - Inflammatory responses of RAW 264.7 macrophages upon exposure to
nanoparticles: Role of ROS-NF kappa B signaling pathway
VL - 5
ID - 6544
ER -
TY - JOUR
AB - Recent advances in particle-forming chemistries used for developing
nanotechnology has not only widened novel applications for nanoscale materials but
also has provided significant concern regarding their biological effects. The
present study investigates the inflammatory responses of RAW 264.7 mouse
macrophages exposed to nanoparticles (NPs, 5 μg/ml) of varied sizes including
silver (Ag), aluminum (Al), carbon black (CB), carbon-coated silver (CAg) and gold
(Au). A significant increase in IL-6, reactive oxygen species (ROS) generation,
nuclear translocation of nuclear factor-kappa B (NF-κB), induction of
cyclooxygenase-2 (COX-2), and tumor necrosis factor-alpha (TNF-α) expression was
observed in macrophages with maximum response found in cells exposed to Ag NPs
followed by Al, CB and CAg. These pro-inflammatory effects of NPs were dependent on
size and duration of exposure and comparable to those induced by lipopolysaccharide
(LPS), a known inflammatory mediator. Au NPs, on the other hand, induced small but
significant inflammatory responses in macrophages upon prolonged exposure. These
studies reveal that Ag NPs exhibit higher propensity in inducing inflammation,
mediated by ROS and NF-κB signaling pathways and leading to the induction of COX-2,
TNF-α and IL-6. However, no such prominent pro-inflammatory responses were observed
with Au NPs, suggesting their bio-compatibility. © 2011 Informa UK, Ltd.
AU - Nishanth, R. P.
AU - Jyotsna, R. G.
AU - Hussain, S. M.
AU - Reddanna, P.
DB - Scopus
DO - 10.3109/17435390.2010.541604
IS - 4
KW - Cyclooxygenase-2
Inflammation
Nanoparticles
NF-κB
ROS
Active Transport, Cell Nucleus
Aluminum
Animals
Cell Line, Transformed
Cell Survival
Cyclooxygenase 2
Interleukin-6
Lipopolysaccharides
Macrophages
Mice
NF-kappa B
Particle Size
Signal Transduction
Silver
Soot
Toxicity Tests
aluminum
carbon
cyclooxygenase 2
gold nanoparticle
interleukin 6
lipopolysaccharide
nanoparticle
silver nanoparticle
animal cell
article
biocompatibility
cell viability
controlled study
cytokine production
enzyme induction
inflammation
macrophage
mouse
nanotechnology
nonhuman
particle size
priority journal
signal transduction
M3 - Article
PY - 2011
SP - 502-516
ST - Inflammatory responses of RAW 264.7 macrophages upon exposure to
nanoparticles: Role of ROS-NFκB signaling pathway
T2 - Nanotoxicology
TI - Inflammatory responses of RAW 264.7 macrophages upon exposure to
nanoparticles: Role of ROS-NFκB signaling pathway
80054064195&doi=10.3109%2f17435390.2010.541604&partnerID=40&md5=5e011d683da78e67c53
b94fbea63b4ec
VL - 5
ID - 5740
ER -
TY - JOUR
AB - SUMMARY: Skeletal muscle injury is an acute inflammatory condition caused by
an inflammatory response. To reduce inflammatory cell infiltration and relieve
skeletal muscle injury, efficient treatment is urgently needed. Nitric oxide is a
free radical molecule reported to have anti-inflammatory effects. In this study, we
showed that NO could inhibit the inflammatory response of C2C12 cells in vitro and
protect rat skeletal muscle injury from notexin in vivo. NO synthase inhibitor (L-
NG-Nitroarginine Methyl Este?L-NAME) and NO donor (sodium nitroprusside dehydrate ?
SNP) were used to explore the vital role of lipopolysaccharides (LPSs) in LPS-
stimulated C2C12 myoblasts.The expression of IL-18 and IL-1b was upregulated by L-
NAME and downregulated by SNP, as indicated by the ELISA results. NO can reduce
ASC, Caspase-1, and NLRP3 mRNA and protein levels. Furthermore, NO was detected in
the rat model. The results of immunohistochemical staining showed that the
production of DMD decreased. We conducted qRT-PCR and western blotting to detect
the expression of Jo-1, Mi-2, TLR2, and TLR4 on day 6 post injury following
treatment with L-NAME and SNP. The expression of Jo-1, Mi-2, TLR2, and TLR4 was
upregulated by L-NAME and significantly reversed by SNP. NO can alleviate C2C12
cell inflammatory responses and protect rat skeletal muscle injury from notexin.
RESUMEN: La lesión del músculo esquelético es una afección inflamatoria aguda
causada por una respuesta inflamatoria. Para reducir la infiltración de células
inflamatorias y aliviar la lesión del músculo esquelético es necesario un
tratamiento eficaz. El óxido nítrico es una molécula de radicales libres que tiene
efectos antiinflamatorios. En este estudio, demostramos que el ON podría inhibir la
respuesta inflamatoria de las células C2C12 in vitro y proteger la lesión del
músculo esquelético de rata de la notexina in vivo. El inhibidor de ON sintasa (L-
NG-nitroarginina metil este, L-NAME) y el donante de ON (nitroprusiato de sodio
deshidratado, SNP) se utilizaron para explorar el papel vital de los
lipopolisacáridos (LPS) en los mioblastos C2C12 estimulados por LPS. La expresión
de IL- 18 e IL-1b fue regulada positivamente por L-NAME y regulada negativamente
por SNP, como indican los resultados de ELISA. El ON puede reducir los niveles de
proteína y ARNm de ASC, Caspasa-1 y NLRP3. Además, se detectó ON en el modelo de
rata. Los resultados de la tinción inmunohistoquímica mostraron que disminuyó la
producción de DMD. Realizamos qRT-PCR y transferencia Western para detectar la
expresión de Jo-1, Mi-2, TLR2 y TLR4 el día 6 después de la lesión después del
tratamiento con L-NAME y SNP. La expresión de Jo-1, Mi-2, TLR2 y TLR4 fue regulada
positivamente por L- NAME y significativamente revertida por SNP. El ON puede
aliviar las respuestas inflamatorias de las células C2C12 en ratas, y proteger la
lesión del músculo esquelético de la notexina.
AD - Niu, Shu-liang
Basic Medical College of Xinjiang Medical University. Department of Anatomy.
Urumqi. CN
Jin, Xiu-Fang
Basic Medical College of Xinjiang Medical University. Department of Anatomy. CN
Yang, Tao
The Fourth Affiliated Hospital of Xinjiang Medical University. Department of Spinal
Surgery. Urumqi. CN
Chen, Zhi-Hui
The Second Affiliated Hospital of Shaanxi University of Chinese Medicine.
Department of Traumatology. Xianyang. CN
Wang, Guang-Dong
The Fourth Affiliated Hospital of Xinjiang Medical University. Department of Spinal
Surgery. Urumqi. CN
Liang, Ying
School of Public Health Xinjiang Medical University. Department of Nutrition and
Food Hygiene. Urumqi. CN
Yan, Jie
Central South University School of Basic Medical Sciences. Changsha. CN
AU - Niu, Shu-liang
AU - Jin, Xiu-Fang
AU - Yang, Tao
AU - Chen, Zhi-Hui
AU - Wang, Guang-Dong
AU - Liang, Ying
AU - Yan, Jie
C1 - 20220812
DA - 2022/02
DB - LILACS
DO - 10.4067/S0717-95022022000100251
IS - 1
KW - C2C12
Inflamación
Inflamasoma
Inflammasome
Inflammation
Lesión muscular
Muscle injury
Nitric oxide
Óxido nítrico
LA - en
PY - 2022
SN - 0717-9367
SP - 251-260
ST - Effects of nitric oxide on C2C12 cell inflammatory responses and notexin-
induced muscle injury
TI - Effects of nitric oxide on C2C12 cell inflammatory responses and notexin-
induced muscle injury
TT - Efectos del óxido nítrico en las respuestas inflamatorias de las células
C2C12 y la lesión muscular inducida por notexina
VL - 40
ID - 4917
ER -
TY - JOUR
AB - Aim: The aim of the study is to synthesis Hybanthus enneaspermus mediated
silver nanoparticles and to evaluate the cytotoxic and antioxidant potential.
Introduction: Hybanthus enneaspermus (F. Muell) belongs to the family Violaceae.
The plant Hybanthus enneaspermus is reported to have antimicrobial, anticonvulsant,
nephroprotective, hepatoprotective, anti-inflammatory, aphrodisiac, aldose
reductase inhibitory and free radical scavenging activities. Ag NPs produce
reactive oxygen species and free radicals causing apoptosis (cell death) thereby
preventing their replication. Many researchers have correlated the plants
antioxidant potential with their phenolic constituents. Antioxidants have
resistance against oxidative stress by scavenging free radicals, thereby preventing
many diseases. Materials and Methods: Cytotoxic activity: Brine shrimp eggs were
obtained from the new aqua laboratory. Filtered artificial seawater was prepared,
then shrimp eggs were added into the dark side of the chamber while there was a
lamp above the other side to attract the hatched shrimp. Two days were allowed for
shrimp to mature, after two days the shrimp Larva was ready. 10 brine shrimps were
added accordingly to the silver nanoparticle. It added 5, 10, 15, 20 micrometer and
it was left for 24 hrs and observation was made. Antioxidant activity: 50%
methanol, DPPH solution and Hybanthus enneaspermus mediated silver nanoparticles
were added in 5 test tubes ranging from 10-50 microliters and kept in a dark place
for 10 minutes and the reading was recorded using photometry. Results and
Discussion: In the cytotoxic activity as the concentration of the silver
nanoparticle increases the percentage of lethality of brine shrimps also increases.
In the antioxidant activity as the concentrations of silver nanoparticles increases
the percentage of inhibition also increases. Conclusion: With the help of the study
we can conclude that silver nanoparticles of Hybanthus enneaspermus possess both
cytotoxic and antioxidant activity. © 2020 Society for Biology and Biotechnology.
AU - Nivethitha, S.
AU - Arivarasu, L.
DB - Scopus
IS - 31-32
KW - Antioxidant activity
BSLA
Cytotoxic activity
DPPH
Hybanthus enneaspermus
Silver nanoparticle
M3 - Article
PY - 2020
SP - 104-110
ST - Cytotoxic and antioxidant potential of hybanthus enneaspermus mediated silver
nanoparticle
T2 - Plant Cell Biotechnology and Molecular Biology
TI - Cytotoxic and antioxidant potential of hybanthus enneaspermus mediated silver
nanoparticle
85092937779&partnerID=40&md5=571fd96d16430eb730de86ea0d12867a
VL - 21
ID - 5274
ER -
TY - JOUR
AB - In recent years, research on silver nanoparticles (AgNPs) has attracted
considerable interest among scientists because of, among other things, their
alternative application to well-known medical agents with antibacterial properties.
The size of the silver nanoparticles ranges from 1 to 100 nm. In this paper, we
review the progress of research on AgNPs with respect to the synthesis,
applications, and toxicological safety of AgNPs, and the issue of in vivo and in
vitro research on silver nanoparticles. AgNPs' synthesis methods include physical,
chemical, and biological routes, as well as "green synthesis". The content of this
article covers issues related to the disadvantages of physical and chemical
methods, which are expensive and can also have toxicity. This review pays special
attention to AgNP biosafety concerns, such as potential toxicity to cells, tissues,
and organs.
AN - WOS:000955548000001
AU - Noga, M.
AU - Milan, J.
AU - Frydrych, A.
AU - Jurowski, K.
C7 - 5133
DA - MAR
DO - 10.3390/ijms24065133
IS - 6
PY - 2023
SN - 1422-0067
ST - Toxicological Aspects, Safety Assessment, and Green Toxicology of Silver
Nanoparticles (AgNPs)-Critical Review: State of the Art
TI - Toxicological Aspects, Safety Assessment, and Green Toxicology of Silver
Nanoparticles (AgNPs)-Critical Review: State of the Art
VL - 24
ID - 5911
ER -
TY - JOUR
AB - Engineered nanomaterials are emerging functional materials with
technologically interesting properties and a wide range of promising applications,
such as drug delivery devices, medical imaging and diagnostics, and various other
industrial products. However, concerns have been expressed about the risks of such
materials and whether they can cause adverse effects. Studies of the potential
hazards of nanomaterials have been widely performed using cell models and a range
of in vitro approaches. In the present review, we provide a comprehensive and
critical literature overview on current in vitro toxicity test methods that have
been applied to determine the mechanisms underlying the cytotoxic effects induced
by the nanostructures. The small size, surface charge, hydrophobicity and high
adsorption capacity of nanomaterial allow for specific interactions within cell
membrane and subcellular organelles, which in turn could lead to cytotoxicity
through a range of different mechanisms. Finally, aggregating the given information
on the relationships of nanomaterial cytotoxic responses with an understanding of
its structure and physicochemical properties may promote the design of biologically
safe nanostructures.
AN - WOS:000342202000015
AU - Nogueira, D. R.
AU - Mitjans, M.
AU - Rolim, C. M. B.
AU - Vinardell, M. P.
DA - JUN
DO - 10.3390/nano4020454
IS - 2
PY - 2014
SN - 2079-4991
SP - 454-484
ST - Mechanisms Underlying Cytotoxicity Induced by Engineered Nanomaterials: A
Review of In Vitro Studies
T2 - NANOMATERIALS
TI - Mechanisms Underlying Cytotoxicity Induced by Engineered Nanomaterials: A
Review of In Vitro Studies
VL - 4
ID - 6324
ER -
TY - JOUR
AB - Due to rapidly increasing resistance development against conventional
antibiotics, finding novel approaches for the treatment of infections has emerged
as a key health issue. Antimicrobial peptides (AMPs) have attracted interest in
this context, and there is by now a considerable literature on the identification
such peptides, as well as on their optimization to reach potent antimicrobial and
anti-inflammatory effects at simultaneously low toxicity against human cells. In
comparison, delivery systems for antimicrobial peptides have attracted considerably
less interest. However, such delivery systems are likely to play a key role in the
development of potent and safe AMP based therapeutics, e.g., through reducing
chemical or biological degradation of AMPs either in the formulation or after
administration, by reducing adverse side-effects, by controlling AMP release rate,
by promoting biofilm penetration, or through achieving co-localization with
intracellular pathogens. Here, an overview is provided of the current understanding
of delivery systems for antimicrobial peptides, with special focus on AMP-carrier
interactions, as well as consequences of these interactions for antimicrobial and
related biological effects of AMP-containing formulations. (C) 2017 Elsevier B.V.
AN - WOS:000399519300002
AU - Nordstrom, R.
AU - Malmsten, M.
DA - APR
DO - 10.1016/j.cis.2017.01.005
PY - 2017
SN - 0001-8686
1873-3727
SP - 17-34
ST - Delivery systems for antimicrobial peptides
T2 - ADVANCES IN COLLOID AND INTERFACE SCIENCE
TI - Delivery systems for antimicrobial peptides
VL - 242
ID - 6674
ER -
TY - JOUR
AB - With recent progress in the manufacture and applications of nickel oxide
nanoparticles (NiO NPs), concerns about their adverse effects are increasing.
Hesperidin (HSP) is a citrus flavonoid that has a potent anti-inflammatory,
antioxidant and free radical scavenging activities. This study aims to investigate
the protective effect of HSP against testicular and spermatological damages induced
by NiO NPs in male rats. Forty rats were randomly and equally divided into four
groups: control, NiO NPs, HSP and NiO NPs + HSP. NiO NPs (100 mg/kg) and/or HSP
(100 mg/kg) were given daily by gavage for 60 days. Exposure to NiO NPs induced
marked reproductive toxicity in male rats that was manifested by increased sperm
abnormalities and deterioration of sperm motility, count and viability. NiO NPs
also increased lipid peroxidation and negatively affected the cellular antioxidant
defense system in the testis of rats. The level of serum testosterone hormone was
increased in NiO NPs-exposed rats. qPCR showed a marked downregulation in
expression of steroidogenesis-related genes (CYP11A1, HSD3B and STAR) and a
significant upregulation in expression of apoptosis-related gene (caspase-9) in
testicular tissue of rats. Various pathological lesions and an increase in the
number of PCNA-positive immune-reactive cells were also noticed in the testis of
NiO NPs-exposed rats. Co-administration of HSP significantly ameliorated most of
the NiO NPs-induced testicular damages and improved male fertility in rats.
AN - WOS:000800141700001
AU - Noshy, P. A.
AU - Khalaf, A. A. A.
AU - Ibrahim, M. A.
AU - Mekkawy, A. M.
AU - Abdelrahman, R. E.
AU - Farghali, A.
AU - Tammam, A. A.
AU - Zaki, A. R.
C6 - MAY 2022
C7 - 153208
DA - MAY 15
DO - 10.1016/j.tox.2022.153208
PY - 2022
SN - 0300-483X
1879-3185
ST - Alterations in reproductive parameters and steroid biosynthesis induced by
nickel oxide nanoparticles in male rats: The ameliorative effect of hesperidin
T2 - TOXICOLOGY
TI - Alterations in reproductive parameters and steroid biosynthesis induced by
nickel oxide nanoparticles in male rats: The ameliorative effect of hesperidin
VL - 473
ID - 6423
ER -
TY - JOUR
AB - Studies have demonstrated that the prolonged use of corticoids can delay the
healing process, affecting re-epithelialization, neovascularization and collagen
synthesis. As the fins of teleost fish contain a large amount of collagen, the aim
of the present study was to investigate the effect of dexamethasone (anti-
inflammatory and glucocorticoid steroid widely used in the treatment of rheumatic
diseases) during the regeneration process in the caudal fin of specimens of carp
(Cyprinus carpio). For such, two glass aquaria were used – one for a group of fish
treated with dexamethasone (Henrifarma) in a 20 mg/L concentration and the other
for the control group. The caudal fins were amputated transversally and fish
remained in their respective aquaria until regeneration occurred. Samples of
regenerating fins were collected on days 1, 2, 4, 6, 8 and 10 after amputation. The
fins in the control group regenerated normally and grew within the expected in time
course. The fins in the group treated with dexamethasone were significantly smaller
in comparison to the control group at every evaluation time. Thus, it was possible
to verify that, at this concentration of dexamethasone, the regeneration of the
caudal fins was delayed, but not completely inhibited. The results show that the
caudal fin is a good model for histological studies on regeneration and the action
of drug toxicity, but it’s also of great importance the interaction with further
studies for a better knowledge and understanding of all the changes in all the
phases.
Estudos mostram que corticóides usados por longos períodos podem atrasar o processo
de cicatrização, influenciando na reepitelização, na neovascularização e na síntese
do colágeno. Os constituintes das nadadeiras dos peixes teleósteos contêm grande
quantidade de colágeno e assim o objetivo do presente trabalho foi estudar o efeito
da dexametasona (um antiinflamatório e glicocorticóide esteróide bastante utilizado
no tratamento de doenças reumáticas) durante o processo regenerativo das nadadeiras
caudais das carpas (Cyprinus carpio). Para isso, foram montados dois aquários de
vidro, um para o grupo controle e outro para o grupo tratado com a dexametasona
(Henrifarma) na concentração de 20mg/L. Os peixes distribuídos nesses aquários
tiveram suas nadadeiras caudais amputadas transversalmente e permaneceram nos
respectivos aquários para que ocorresse a regeneração. Foram feitas coletas das
nadadeiras em regeneração em intervalos de 1, 2, 4, 6, 8 e 10 dias após a
amputação. Foi observado que nos peixes do grupo controle, as nadadeiras
regeneraram normalmente e cresceram o esperado em cada intervalo de tempo. No
entanto, foi verificado que nos peixes do grupo tratado com dexametasona, em cada
intervalo analisado, as nadadeiras regeneradas dos peixes expostos à droga eram
menores que a medida das nadadeiras dos peixes do grupo controle. Assim, foi
possível verificar que, nessa concentração de dexametasona, a regeneração das
nadadeiras caudais foi mais lenta, mas não ocorreu a total inibição da regeneração.
Dessa forma, os resultados comprovam que a nadadeira caudal é um bom modelo para
estudos histológicos sobre a regeneração e a ação da toxicidade de drogas, mas,
também, é de grande importância a interação com estudos mais aprofundados para se
conhecer e compreender melhor todas as alterações em todas as fases.
AD - Ochandio, BS.
Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências.
Departamento de Biologia. Rio Claro. BR
Bechara, IJ.
Parise-Maltempi, PP.
AU - Ochandio, B. S.
AU - Bechara, I. J.
AU - Parise-Maltempi, P. P.
C1 - 20150625
DA - 2015/05
DB - LILACS
DO - 10.1590/1519-6984.16813
IS - 2
KW - Caudal fin
Collagen
Colágeno
Dexametasona
Dexamethasone
Fish
Nadadeira caudal
Peixe
Regeneração tecidual
Tissue regeneration
LA - en
PY - 2015
SN - 1519-6984
SP - 442-450
ST - Dexamethasone action on caudal fin regeneration of carp Cyprinus carpio
(Linnaeus, 1758)
T2 - Braz. j. biol
TI - Dexamethasone action on caudal fin regeneration of carp Cyprinus carpio
(Linnaeus, 1758)
TT - Estudo da ação da dexametasona na regeneração da nadadeira caudal de Cyprinus
carpio (carpa) (Linnaeus, 1758)
69842015000200027
VL - 75
ID - 4935
ER -
TY - JOUR
AB - Peri-implantitis is an inflammatory disease with a relevant focus on the
long-term success of dental implants and implant-supported prostheses. The present
study focuses on the antibacterial effect of the silver nanoparticle and
investigated the suppression of dental plaque adhesion on implant abutment and/or
superstructure by micro-wave assistant nanosilver coating in vivo and in vitro.
Nanosilver coating on pure titanium was prepared by microwave-assisted synthesis,
and characterized by scanning electron microscopy and energy-dispersive X-ray
spectroscopy. In vitro studies were conducted to analyze biocompatibility using MTS
assay and fluorescence microscopy with human gingival fibroblasts to evaluate
antibacterial activity. During the in vivo study, nanosilver coating was applied to
the healing abutments, and the prevention of plaque accumulation on nanosilver
coating was confirmed by a split-mouth randomized clinical trial. The aggregation
of nano-sized particles was found on the titanium surface with an antibacterial
effect. The coating had no cytotoxic effect on human gingival fibroblasts. The
result of the clinical trial showed that the coating suppressed the dental plaque
adhesion on the healing abutments. Nanosilver coating is a promising material with
antibacterial properties and can be used for implant abutments and prostheses for
preventing peri-implantitis. © 2020 by the authors.
AU - Odatsu, T.
AU - Kuroshima, S.
AU - Sato, M.
AU - Takase, K.
AU - Valanezhad, A.
AU - Naito, M.
AU - Sawase, T.
C7 - 347
DB - Scopus
IS - 6
KW - Antibacterial
Microwave synthesis
Nanosilver
Peri-implantitis
nanocoating
silver nanoparticle
titanium
adult
aged
Article
biocompatibility
cell adhesion
cell proliferation
clinical article
colony forming unit
controlled study
female
fibroblast
human
human cell
male
microwave assisted synthesis
microwave radiation
MTS assay
priority journal
radiological procedures
tooth plaque
M3 - Article
PY - 2020
SP - 1-11
ST - Antibacterial properties of nano-ag coating on healing abutment: An in vitro
and clinical study
T2 - Antibiotics
TI - Antibacterial properties of nano-ag coating on healing abutment: An in vitro
and clinical study
85090605696&doi=10.3390%2fantibiotics9060347&partnerID=40&md5=fcc39e19478a50aa6c62a
d6758c0c98d
VL - 9
ID - 5350
ER -
TY - JOUR
AB - Chronic inflammatory wounds pose therapeutic challenges in the biomedical
field. Polymeric nanofibrous matrices provide extracellular-matrix-like structures
to facilitate wound healing; however, wound infection and the subsequent
accumulation of reactive oxygen species (ROS) delay healing. Therefore, we herein
developed electrospun nanofibers (NFs), composed of chitosan-stabilized Prussian
blue (PBChi) nanoparticles (NPs) and poly(vinyl alcohol) (PVA), with ROS scavenging
activity to impart antioxidant and wound healing properties. The PBChi NPs were
prepared using chitosan with different molecular weights, and their weight ratio
with respect to PVA was optimized to yield PBChi-NP-coated PVA NFs with well-
defined NF structures. In situ and in vitro antioxidant activity assays showed that
the PBChi/PVA NFs could effectively remove ROS. Particularly, PBChi/PVA NFs with a
lower chitosan molecular weight exhibited greater antioxidant activity. The
hydroxyl radical scavenging activity of PBChi10k/PVA NFs was 60.4 %, approximately
two-fold higher than that of PBChi100k/PVA NFs. Further, at the concentration of 10
mu g/mL, they could significantly lower the in vitro ROS level by up to 50.7 %. The
NFs caused no significant reduction in cell viability, owing to the excellent
biocompatibility of PVA with PBChi NPs. Treatment using PBChi/PVA NFs led to faster
cell proliferation in in vitro scratch wounds, reducing their size from 202 to 162
mu m. The PBChi/PVA NFs possess notable antioxidant and cell proliferation
properties as ROS-scavenging wound dressings.
AN - WOS:000863212800008
AU - Oh, H.
AU - Son, D.
AU - Lee, J. S.
AU - Kim, M.
AU - Sung, D. K. Y.
AU - Lee, H. K.
AU - Il Choi, W.
C6 - AUG 2022
DA - OCT 31
DO - 10.1016/j.ijbiomac.2022.08.033
PY - 2022
SN - 0141-8130
1879-0003
SP - 835-843
ST - Reactive oxygen species scavenging nanofibers with chitosan-stabilized
Prussian blue nanoparticles for enhanced wound healing efficacy
TI - Reactive oxygen species scavenging nanofibers with chitosan-stabilized
Prussian blue nanoparticles for enhanced wound healing efficacy
VL - 219
ID - 6735
ER -
TY - JOUR
AB - Despite the dangers associated with the increased use of codeine drugs,
limited researches have addressed the specific effects of emzolyn codeine on the
lung. The aim of this study was to assess the histological effects of emzolyn
codeine cough syrup on the lung of Wistar rats and its oxidative stress. Twenty one
(21) Wistar rats were divided into 3 groups labeled T1, T2 and T3. Group T1 served
as control and was given distilled water and diet for 42 days, group T2 was treated
with 0.1 mg/g bodyweight emzolyn codeine cough syrup for 21 days while group T3 was
treated with 0.1 mg/g bodyweight emzolyn codeine cough syrup for 42 days. At the
end of the duration, the wistar rats were sacrificed under anaesthesia and the
lungs were collected after dissection and transferred into 10% buffered formalin.
Sections of the lungs were obtained and processed for histological studies using
Hematoxylin and Eosin stain, Periodic acid Schiff's solution, Phosphotungstic acid
Haematoxylin stain and Methanamine Silver stains. Results from the study suggested
that acute and chronic exposure to emzolyn codeine cough syrup produced significant
(P<0.05) decrease in body weight, edematous aveolar space with marked type 11
pneumocyte,marked hypertrophy (H) of the septa and marked inflammatory cells. The
levels of total antioxidant status (TAS) was also determined using standard
spectrophotometric techniques. The mean MDA of the exposed groups were
significantly higher while the mean levels of SOD, GPx, CAT, and GSH were
significantly lower than the control group. In conclusion, this study confirmed the
risk of increased oxidative stress, pulmonary toxicity and decreased body weight
due to emzolyn codeine cough syrup administration. Thus, indiscriminately and
prolong use emzolyn codeine drug should be avoided and antioxidant supplements are
advised as a prophylactic supportive therapy for adequate measures in preventing
development of oxidative stress-associated complications among exposed individuals.
AN - WOS:000720116200019
AU - Okorie, N.
AU - Obeagu, E. I.
AU - Nnamani, A. D.
AU - Ude, U. A.
AU - Agada, U. N.
AU - Obi, I. A.
AU - Ibiam, G. A.
C7 - 76364
DO - 10.9734/JPRI/2021/v33i49B33359
IS - 49B
PY - 2021
SN - 2456-9119
SP - 228-240
ST - Histopathological Effect of Emzolyn Codein Cough Syrup on Lungs and Its
Oxidative Stress Biomarkers
TI - Histopathological Effect of Emzolyn Codein Cough Syrup on Lungs and Its
Oxidative Stress Biomarkers
VL - 33
ID - 6837
ER -
TY - JOUR
AB - Zinc oxide particles were synthesized in various sizes and shapes, i.e.,
spheres of 40-nm, 200-nm, and 500-nm diameter and rods of 40 center dot 100 nm(2)
and 100 center dot 400 nm(2) (all PVP-stabilized and well dispersed in water and
cell culture medium). Crystallographically, the particles consisted of the
hexagonal wurtzite phase with a primary crystallite size of 20 to 100 nm. The
particles showed a slow dissolution in water and cell culture medium (both neutral;
about 10% after 5 days) but dissolved within about 1 h in two different simulated
lysosomal media (pH 4.5 to 4.8). Cells relevant for respiratory exposure (NR8383
rat alveolar macrophages) were exposed to these particles in vitro. Viability,
apoptosis, and cell activation (generation of reactive oxygen species, ROS, release
of cytokines) were investigated in an in vitro lung cell model with respect to the
migration of inflammatory cells. All particle types were rapidly taken up by the
cells, leading to an increased intracellular zinc ion concentration. The
nanoparticles were more cytotoxic than the microparticles and comparable with
dissolved zinc acetate. All particles induced cell apoptosis, unlike dissolved zinc
acetate, indicating a particle-related mechanism. Microparticles induced a stronger
formation of reactive oxygen species than smaller particles probably due to higher
sedimentation (cell-to-particle contact) of microparticles in contrast to
nanoparticles. The effect of particle types on the cytokine release was weak and
mainly resulted in a decrease as shown by a protein microarray. In the particle-
induced cell migration assay (PICMA), all particles had a lower effect than
dissolved zinc acetate. In conclusion, the biological effects of zinc oxide
particles in the sub-toxic range are caused by zinc ions after intracellular
dissolution, by cell-to-particle contacts, and by the uptake of zinc oxide
particles into cells. Graphical headlights center dot The cytotoxicity of zinc
oxide particles is mainly due to the intracellular release of zinc ions. center dot
The size and shape of zinc oxide micro- and nanoparticles has only small effects on
lung cells in the sub-toxic range. center dot Zinc oxide particles are rapidly
taken up by cells, regardless of their size and shape. center dot Zinc oxide
particles rapidly dissolve after cellular uptake in endolysosomes.
AN - WOS:000590459800001
AU - Olejnik, M.
AU - Kersting, M.
AU - Rosenkranz, N.
AU - Loza, K.
AU - Breisch, M.
AU - Rostek, A.
AU - Prymak, O.
AU - Schurmeyer, L.
AU - Westphal, G.
AU - Koller, M.
AU - Bunger, J.
AU - Epple, M.
AU - Sengstock, C.
C6 - NOV 2020
DA - AUG
DO - 10.1007/s10565-020-09571-z
IS - 4
PY - 2021
SN - 0742-2091
1573-6822
SP - 573-593
ST - Cell-biological effects of zinc oxide spheres and rods from the nano- to the
microscale at sub-toxic levels
TI - Cell-biological effects of zinc oxide spheres and rods from the nano- to the
microscale at sub-toxic levels
VL - 37
ID - 6506
ER -
TY - JOUR
AB - In this study, we aimed to evaluate the effect of Bacillus coagulans and
Lactobacillus casei probiotics on liver damage induced by silver nanoparticles and
expression of Bax, Bcl2 and Caspase 3 genes in rats. 32 adult male Wistar rats were
divided into four healthy groups (control), the group receiving silver
nanoparticles treated with L. casei, the group receiving silver nanoparticles
treated with B. coagulans and the group receiving only silver nanoparticles. The
effect of nanoparticles was induced by intraperitoneal injection of silver
nanoparticles prepared from nettle at a dose of 50 mg/kg and entered the liver
tissue through the bloodstream. Two days after injection, probiotic treatment with
109 CFU was performed by gavage for 30 days. One day after the last gavage, rat
liver tissue weight was assessed. Also, the total amount of RNA was extracted from
treated, and healthy tissues, as well as induced silver nanoparticles tissues, then
evaluated by Real Time PCR. Data were evaluated using one-way Anova, Tukey test.
Based on the biochemical results of this study, exposure of rats to different
concentrations of silver nanoparticles compared with the control group caused a
significant increase in the serum levels of alanine transaminase (ALT) and
aspartate transaminase (AST), alkaline phosphatase (ALP), especially at high
concentrations. Evaluation of damage and histopathological lesions showed that
silver nanoparticles in different concentrations caused different damage to liver
tissue, so that, necrosis, inflammatory cell infiltration and vascular degeneration
were observed at different concentrations by silver nanoparticles. In the present
study, the effects of L. casei cell extract on increasing the expression of Bax
proapoptotic gene and decreasing Bcl2 gene expression in cancer cells and inducing
programmed cell death were shown. In this study, the expression of Bax, Bcl-2 and
Caspase-3 genes in the group receiving silver nanoparticles and in the groups
treated with probiotics showed significant changes compared to the control group.
It can be concluded that the function of silver nanoparticles and the effects of
relative improvement of probiotics are from the internal route of apoptosis and
factors such as dose, nanoparticle size and nanoparticle coating have an important
role in the toxicity of silver nanoparticles, thus the destructive effects on liver
tissue could be increased by increasing the concentration of silver nanoparticles.
© 2023 PAGEPress Publications. All rights reserved.
AU - Oliaei, R.
AU - Keshtmand, Z.
AU - Shabani, R.
C7 - 10673
DB - Scopus
DO - 10.4081/ejtm.2022.10673
IS - 1
KW - Bax
Bcl2
Caspase-3
liver enzymes
probiotics
rat
caspase 3
Lactobacillus casei extract
liver enzyme
probiotic agent
protein Bax
protein bcl 2
silver nanoparticle
adult
animal experiment
animal model
animal tissue
apoptosis
Article
Bacillus coagulans
cell infiltration
controlled study
gene expression
histopathology
inflammatory cell
Lactobacillus casei
liver injury
liver tissue
liver weight
male
nonhuman
protein expression
RNA extraction
Wistar rat
M3 - Article
PY - 2023
ST - The effect of Lactobacillus casei and Bacillus coagulans probiotics on liver
damage induced by silver nanoparticles and expression of Bax, Bcl2 and Caspase 3
genes in male rats
T2 - European Journal of Translational Myology
TI - The effect of Lactobacillus casei and Bacillus coagulans probiotics on liver
damage induced by silver nanoparticles and expression of Bax, Bcl2 and Caspase 3
genes in male rats
85154537323&doi=10.4081%2fejtm.2022.10673&partnerID=40&md5=32b6a0c3d18b7f6298003822
a136aa5e
VL - 33
ID - 4962
ER -
TY - JOUR
AB - ABSTRACT Diabetes mellitus (DM) is a disease associated with delayed wound
healing of oral ulcers by increased expression of proinflammatory cytokines and
cellular apoptosis. Objective to evaluate the influence of Tumor Necrosis Factor
alpha (TNF-α) and apoptosis in rats with DM treated with chamomile extract or
triamcinolone. Material and Methods Wistar male rats (210.0±4.2 g) were divided
into five groups: negative control group (NCG) without diabetes; positive control
group (PCG) with DM (alloxan, 45 mg/kg); and groups treated with chamomile extract
(normoglycemic= NCG group and diabetic= DCG group) and with triamcinolone (TG).
Traumatic ulcers were performed on all animals that received topical triamcinolone,
chamomile extract or saline 12/12 hours for ten days. Results On days five and ten
the animals were euthanized and the ulcers were analyzed by light microscopy, TUNEL
assay, and immunohistochemically (TNF-α). The NCG (p=0.0062), PCG (p=0.0285), NCG
(p=0.0041), and DCG (p<0.0001) groups were completely healed on the 10th day,
however, there was no healing on the TG (p=0.5127) group. The TNF-α expression
showed a significant reduction from the 5th to the 10th day in NCG (p=0.0266) and
DCG (p=0.0062). In connective tissue, the TUNEL assay showed a significant
reduction in the number of positive cells in NCG (p=0.0273) and CNG (p=0.0469) and
in the epithelium only in CDG (p=0.0320). Conclusions Chamomile extract can
optimize the healing of traumatic oral ulcers in diabetic rats through the
reduction of apoptosis in the epithelium and TNF-α expression.
AD - OLIVEIRA, Bruna Vasconcelos
Universidade Federal do Ceará. Setor de Patologia Oral. Departamento de Odontologia
Clínica. Fortaleza. BR
BARROS SILVA, Paulo Goberlânio
NOJOSA, Jacqueline de Santiago
BRIZENO, Luiz André Cavalcante
FERREIRA, Jamile Magalhães
SOUSA, Fabrício Bitú
MOTA, Mário Rogério Lima
ALVES, Ana Paula Negreiros Nunes
AU - Oliveira, Bruna Vasconcelos
AU - Barros Silva, Paulo Goberlânio
AU - Nojosa, Jacqueline de Santiago
AU - Brizeno, Luiz André Cavalcante
AU - Ferreira, Jamile Magalhães
AU - Sousa, Fabrício Bitú
AU - Mota, Mário Rogério Lima
AU - Alves, Ana Paula Negreiros Nunes
C1 - 20160729
DA - 2016/06
DB - LILACS
DO - 10.1590/1678-775720150481
IS - 3
KW - Chamomile
Diabetes mellitus
Matricaria
Oral ulcer
Triamcinolone
LA - en
PY - 2016
SN - 1678-7757
SP - 278-290
ST - TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria
recutita L. extract and triamcinolone on oral ulcer in diabetic rats
T2 - J. appl. oral sci
TI - TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria
recutita L. extract and triamcinolone on oral ulcer in diabetic rats
77572016000300278
VL - 24
ID - 4932
ER -
TY - GEN
AB - Os nódulos de tireóide são frequentes no mundo todo, principalmente entre as
mulheres. A preocupação maior no seu estudo é afastar a presença de neoplasia
maligna, que corresponde a aproximadamente 10% de todos os casos. Alguns
procedimentos médicos têm sido usados para se chegar ao diagnóstico pré-operatório
dos nódulos tireoideanos, dentre os quais a cintilografia, a ultrassonografia e,
mais recentemente, a punção aspirativa com agulha fina... Na tentativa de colaborar
para o esclarecimento desse tema, estudamos 102 amostras de tireóide arquivadas no
banco de tumores do Hospital AC Camargo, formando com elas dois grupos de
pacientes, um com carcinoma e tireoidite (70 pacientes) e outro, somente com
carcinoma (32 pacientes). Tendo em vista a importância da caracterização objetiva
do fenômeno inflamatório, subclassificamos, por parâmetros histológicos, a
tireoidite em leve, moderada e intensa. Foi realizado estudo imunoistoquímico para
avaliação de 16 proteínas relacionadas à presença de células indiferenciadas (p63),
à via das MAPKs (Ras, AKT-1e ERK1/2), às moléculas de adesão (E-caderina e CD44), à
ativação da via de sinalização Wnt (beta-catenina), à via do receptor de morte
(Fas-L e caspase 8), às moléculas ligadas à indução de interleucinas (iNOS e COX-
2), aos fatores de crescimento e diferenciação celulares (galectina 3 e VEGF) e aos
índices de proliferação celular e apoptose (Ki-67, caspase 3 clivada e Fas). Além
disso, fizemos a pesquisa da mutação V600E do gene BRAF por pirossequenciamento e a
pesquisa dos rearranjos cromossômicos RET/PTC1 e RET/PTC3 por RT-PCR. Os resultados
evidenciaram diferenças estatisticamente significativas na expressão de Ras,
ERK1/2, CD44, COX-2 e Fas entre os grupos com e sem tireoidite. Essas diferenças
também validaram a subclassificação histológica para a intensidade da tireoidite,
ao demonstrar que quanto mais intensa ela se apresentou, maior foi a expressão
imunoistoquímica dessas proteínas.
Thyroid nodules are common throughout the world, mainly in women. The principal
focus of their study is to exclude the possibility of a malignant neoplasm, which
is found in approximately 10% of all cases. Various medical procedures, such as
scintillography, ultrasonography, and, more recently, fine needle aspiration biopsy
are used pre-operatively to diagnose thyroid nodules. However, the definitive
diagnosis is still the pathologic diagnosis, often preceded by examination of
multiple frozen sections. One of the limiting factors of this method is the
presence of autoimmune thyroiditis, as the morphological tissue alterations
associated with this inflammation cause problems in the macroscopic and microscopic
evaluation of the tissue. Not only during the frozen section analysis, but also
during the definitive evaluation, the association between thyroiditis and papillary
carcinoma is a challenge, as there is still no consensus about the nature of this
association. In an attempt to help clarify this situation, we studied samples of
thyroid tissue from 102 patients, collected from the archives of the Tumor Bank of
the AC Camargo Hospital, divided into two groups; the first group consisted of 70
patients with both carcinoma and thyroiditis, while the second group of 32 patients
had only carcinoma. Due to the importance of an objective characterization of the
inflammatory process, specifically in this research, histological parameters were
used to sub classify the thyroiditis as mild, moderate or severe. We used
immunihistochemical methods to study 16 proteins related to: the presence of
undifferentiated cells (p63); the MAPK pathway (Ras, AKT-1 and ERK1/2); adhesion
molecules (E-caderin and CD44); the Wnt signal activation pathway (beta-catenin);
the death receptor pathway (Fas-L and caspase 8); molecules associated with
induction of interleukins (iNOS and COX-2); factors of growth and cellular
differentiation (Galactin 3 and VEGF); and indices of cellular proliferation and
apoptosis (Ki67, activated caspase 3 and Fas). As well, we used pyrosequencing to
study the V600E mutation of the BRAF gene, and RT-PCR to evaluate rearrangements of
chromosomes RET/PTC1 and RET/PTC3. Our results showed statistically significant
differences between the groups with and without thyroiditis in the expression of
Ras, ERK 1/2, CD44, COX-2 and Fas. These results also validated the histological
sub classification used to grade the intensity of the thyroiditis; the more intense
the thyroiditis, the greater was the immunihistochemical expression of these
proteins.
AU - Oliveira, Paulo Roberto Grimaldi
C1 - 20100524
C8 - lil-553376
DA - 2009/00
DB - LILACS
LA - pt
PY - 2009
SP - 137-137
ST - Carcinoma papilífero da tireóide: estudo comparativo entre os casos usuais e
aqueles associados à tireoidite autoimune
TI - Carcinoma papilífero da tireóide: estudo comparativo entre os casos usuais e
aqueles associados à tireoidite autoimune
TT - Papillary carcinoma of the thyroid: a comparison of typical cases with those
associated autoimmune thyroiditis
UR - http://accamargo.phlnet.com.br/Doutorado/2009/PGrimaldi/PGrimaldi.pdf
ID - 4951
ER -
TY - JOUR
AB - Zinc (Zn) has emerged as a promising bioresorbable stent material because of
its satisfactory corrosion behavior and excellent biocompatibility. However, for
load-bearing implant applications, alloying is required to boost its mechanical
properties as pure Zn exhibits poor strength. Unfortunately, an increase in
inflammation relative to pure Zn is a commonly observed side effect of Zn alloys.
Consequently, the development of a Zn-based alloy that can simultaneously feature
improved mechanical properties and suppress inflammatory responses is a big
challenge. Here, a bioresorbable, biocompatible Zn-Ag-based quinary alloy was
comprehensively evaluated in vivo, in comparison to reference materials. The
inflammatory and smooth muscle cellular response was characterized and correlated
to metrics of neointimal (NI) growth. We found that implantation of the quinary
alloy was associated with significantly improved inflammatory activities relative
to the reference materials. Additionally, we found that inflammation, but not
smooth muscle cell hyperplasia, significantly correlates to NI growth for Zn
alloys. The results suggest that inflammation is the main driver of NI growth for
Zn-based alloys and that the quinary Zn-Ag-Mn-Zr-Cu alloy may impart inflammation-
resistance properties to arterial implants. © 2020 American Chemical Society.
AU - Oliver, A. A.
AU - Guillory, R. J.
AU - Flom, K. L.
AU - Morath, L. M.
AU - Kolesar, T. M.
AU - Mostaed, E.
AU - Sikora-Jasinska, M.
AU - Drelich, J. W.
AU - Goldman, J.
DB - Scopus
DO - 10.1021/acsabm.0c00740
IS - 10
biodegradable stent
inflammation
neointima
zinc alloy
Biocompatibility
Cells
Copper alloys
Corrosive effects
Manganese alloys
Metal implants
Muscle
Pathology
Silver alloys
Zircaloy
Cellular response
Corrosion behavior
Inflammatory activity
Reference material
Resistance properties
Smooth muscle cells
Zn-based alloys
Zinc alloys
M3 - Article
PY - 2020
SP - 6779-6789
ST - Analysis of Vascular Inflammation against Bioresorbable Zn-Ag-Based Alloys
T2 - ACS Applied Bio Materials
TI - Analysis of Vascular Inflammation against Bioresorbable Zn-Ag-Based Alloys
85096334801&doi=10.1021%2facsabm.0c00740&partnerID=40&md5=a632335302fba9d67d11f17fa
a589e47
VL - 3
ID - 5341
ER -
TY - JOUR
AB - The local effects of silver-coated polyurethane catheters and Dacron ®
material were compared to uncoated polyurethane catheters and Dacron® material in a
long-term implantation test using rabbits. The tissue-implant interaction was
analysed by investigating the type and number of inflammatory cells, capillaries,
fat tissue, the extent of fibrosis, thickness of the fibrous capsule, number and
distribution of silver particles, and the size of giant cells. Silver-coated and
uncoated materials displayed comparable signs of inflammation and tissue reaction.
Biomaterials (1994) 15, (10) 753-758. © 1994.
AU - Oloffs, A.
AU - Grosse-Siestrup, C.
AU - Bisson, S.
AU - Rinck, M.
AU - Rudolph, R.
AU - Gross, U.
DB - Scopus
DO - 10.1016/0142-9612(94)90028-0
IS - 10
Dacron ®
long-term implantation test
polyurethane catheters
Silver coating
tissue reaction
Animal
Catheters, Indwelling
Comparative Study
Male
Materials Testing
Muscles
Polyurethanes
Rabbits
Silver
Animalia
dacron
polyurethan
silver
adipose tissue
animal experiment
animal tissue
capillary
catheter infection
catheterization
cell size
conference paper
fibrosis
giant cell
implantation
inflammatory cell
macrophage
male
nonhuman
priority journal
rabbit
M3 - Article
PY - 1994
SP - 753-758
ST - Biocompatibility of silver-coated polyurethane catheters and silvercoated
Dacron® material
T2 - Biomaterials
TI - Biocompatibility of silver-coated polyurethane catheters and silvercoated
Dacron® material
0028132746&doi=10.1016%2f0142-9612%2894%2990028-
0&partnerID=40&md5=11fed8fbe35b81ae49cbda3e5dc2ebb7
VL - 15
ID - 5790
ER -
TY - JOUR
AB - The local effects of silver-coated polyurethane catheters and Dacron(R)
material were compared to uncoated polyurethane catheters and Dacron(R) material in
a long-term implantation test using rabbits. The tissue-implant interaction was
analysed by investigating the type and number of inflammatory cells, capillaries,
fat tissue, the extent of fibrosis, thickness of the fibrous capsule, number and
distribution of silver particles, and the size of giant cells. Silver-coated and
uncoated materials displayed comparable signs of inflammation and tissue reaction.
AN - WOS:A1994PC34700005
AU - Oloffs, A.
AU - Grossesiestrup, C.
AU - Bisson, S.
AU - Rinck, M.
AU - Rudolph, R.
AU - Gross, U.
DA - AUG
DO - 10.1016/0142-9612(94)90028-0
IS - 10
PY - 1994
SN - 0142-9612
SP - 753-758
ST - BIOCOMPATIBILITY OF SILVER-COATED POLYURETHANE CATHETERS AND SILVER-COATED
DACRON(R) MATERIAL
T2 - BIOMATERIALS
TI - BIOCOMPATIBILITY OF SILVER-COATED POLYURETHANE CATHETERS AND SILVER-COATED
DACRON(R) MATERIAL
VL - 15
ID - 6021
ER -
TY - JOUR
AB - Reproductive derangement and metabolic disorders in human immunodeficiency
virus (HIV) infected persons require a nanoparticle delivery system to convey
antiretroviral drugs to the anatomical sanctuary such as testis. This study
investigated the effects of tenofovir disoproxil fumarate (TDF) loaded silver
nanoparticles (AgNPs) on the testicular oxidative stress, inflammatory cytokines
and histology in male diabetic rats. Thirty-six Sprague-Dawley rats weighing 230 ±
20 g were randomly divided into diabetic and non-diabetic groups (n = 18). Diabetes
was induced using the fructose-streptozotocin (Frt-STZ) rat model. Both groups were
further divided into three (n = 6) and administered distilled water, TDF, or TDF-
AgNP. Results obtained with the TDF-AgNP administration showed a significant
increase (p <.05) in the reduced glutathione and catalase levels. Tumour necrosis
factor-alpha and interleukin 6 were reduced in diabetic rats administered TDF-AgNP.
More so, administration of TDF-AgNP to diabetic rats improved testicular
histoarchitecture in diabetic rats. In addition, diabetic rats administered TDF-
AgNP showed a significant reduction (p <.05) in blood glucose levels. TDF-AgNP to
diabetic rats enhanced testicular antioxidant enzyme, reduced testicular
inflammation, and alleviated structural derangements in the testis. Thus, the
application of AgNP to deliver TDF may alleviate testicular toxicity and
subsequently cater for neglected reproductive dysfunction during the management of
HIV infection. © 2022 The Author(s). Published by Informa UK Limited, trading as
AU - Olojede, S. O.
AU - Lawal, S. K.
AU - Dare, A.
AU - Naidu, E. C. S.
AU - Rennie, C. O.
AU - Azu, O. O.
DB - Scopus
DO - 10.1080/21691401.2022.2042009
IS - 1
KW - Diabetes mellitus
reproductive dysfunction
tenofovir disoproxil fumarate
testis
Animals
Diabetes Mellitus, Type 2
HIV Infections
Male
Metal Nanoparticles
Rats
Silver
Tenofovir
Testis
Cell death
Diseases
Morphology
Viruses
catalase
cytokine
glutathione
interleukin 6
silver nanoparticle
tenofovir disoproxil
metal nanoparticle
silver
tenofovir
Diabetic rats
Fumarates
Metabolic disorders
Reproductive dysfunction
Tenofovir disoproxil fumarate
Type 2 Diabetic Rats
animal cell
animal experiment
animal model
animal tissue
Article
controlled study
diabetes control
drug distribution
evaluation study
Fourier transform mass spectrometry
functional morphology
gene expression
glucose blood level
histology
histopathology
Human immunodeficiency virus infection
male
nonhuman
oxidative stress
rat
room temperature
testis weight
tissue preparation
animal
metabolism
pathology
Sprague Dawley rat
Metal nanoparticles
M3 - Article
PY - 2022
SP - 71-80
ST - Evaluation of tenofovir disoproxil fumarate loaded silver nanoparticle on
testicular morphology in experimental type-2 diabetic rats
TI - Evaluation of tenofovir disoproxil fumarate loaded silver nanoparticle on
testicular morphology in experimental type-2 diabetic rats
85127285946&doi=10.1080%2f21691401.2022.2042009&partnerID=40&md5=e46662582882ad1ce9
2198085cbc7a44
VL - 50
ID - 5149
ER -
TY - JOUR
AB - The intraosseous implantation of Teflon cups as carriers for endodontic
materials were evaluated for suitability as a method for the testing of the
biocompatibility of these materials. Three representative types of materials were
used. Cavities were prepared on both sides between the symphysis and the incisors
in the mandible of 72 guinea pigs, and Teflon cups filled with sealers were placed
in the bone cavity. Empty reversed cups served as controls. A tissue reaction to
the materials consisting of acute and chronic inflammatory cells, resorption and
apposition of bone, presence of material in macrophages, foreign body cells and
vessels, could be differentiated from a reaction to the implanted cup. The
reactions to the surgical trauma in the bone were assessed with those of the
material. It was concluded that the intraosseous method compared with a
subcutaneous tube implantation method involved greater initial tissue damage, but
the intraosseus method had the advantage of reflecting the reactions in bone to the
material. © 1981 American Association of Endodontists.
AU - Olsson, B.
AU - Sliwkowski, A.
AU - Langeland, K.
DB - Scopus
DO - 10.1016/S0099-2399(81)80003-9
IS - 6
KW - Animal
Balsams
Bismuth
Bisphenol A-Glycidyl Methacrylate
Drug Combinations
Female
Guinea Pigs
Gutta-Percha
Mandible
Methacrylates
Methenamine
Silver
Titanium
Zinc Oxide
balsam
biomaterial
bismuth
bisphenol A bis(2 hydroxypropyl) ether dimethacrylate
Bisphenol A Glycidyl Methacrylate
gutta percha
kloroperka
methacrylic acid derivative
methenamine
silver
titanium
zinc oxide
animal
article
drug combination
female
guinea pig
histology
mandible
M3 - Article
PY - 1981
SP - 253-265
ST - Intraosseous implantation for biological evaluation of endodontic materials
TI - Intraosseous implantation for biological evaluation of endodontic materials
0019582725&doi=10.1016%2fS0099-2399%2881%2980003-
9&partnerID=40&md5=388c0bae1666e5c180efbc6944f410f3
VL - 7
ID - 5823
ER -
TY - JOUR
AB - The subcutaneous implantation of Teflon tubes as carriers for endodontic
materials was evaluated for its suitability as a method for the testing of the
biocompatibility of these materials. Three representative types of root canal
sealers were used. Tubes filled with sealers were placed in the subcutaneous tissue
of 143 rats. The observation periods were 14, 30, 90, and 180 days. The occurrence
of acute and chronic inflammatory' cells, presence of material in macro phages,
foreign body giant cells, and vessels, demonstrated that all sealers were
irritating and resorbable. The severity of the tissue response varied widely within
each group. It was concluded that the implantation method was practical for the
qualitative evaluation of endodontic materials, but that a ranking of biocompat-
ibility of materials would be possible only for materials of considerably different
toxicity. © 1981 American Association of Endodontists.
AU - Olsson, B.
AU - Sliwkowski, A.
AU - Langeland, K.
DB - Scopus
DO - 10.1016/S0099-2399(81)80057-X
IS - 8
KW - Animal
Balsams
Bismuth
Drug Combinations
Evaluation Studies
Gutta-Percha
Methenamine
Rats
Silver
Skin
Support, U.S. Gov't, P.H.S.
Titanium
Zinc
Zinc Oxide
balsam
biomaterial
bismuth
gutta percha
kloroperka
methenamine
silver
titanium
zinc
zinc oxide
animal
drug combination
drug effect
histology
rat
review
skin
M3 - Article
PY - 1981
SP - 355-369
ST - Subcutaneous implantation for the biological evaluation of endodontic
materials
TI - Subcutaneous implantation for the biological evaluation of endodontic
materials
0019603318&doi=10.1016%2fS0099-2399%2881%2980057-
X&partnerID=40&md5=d2d0b15452f2818c107c0f0b94f42133
VL - 7
ID - 5814
ER -
TY - JOUR
AB - Silver nanoparticles are well received in the cosmeceutical industry due to
their broad spectrum of pharmacology applications. Research on the therapeutic
properties exhibited by silver nanoparticles revealed that the antimicrobial and
anti-inflammatory properties are the main attraction in the establishment of
nanocosmeceutical products whereby their mechanisms of action are reviewed in this
paper. In addition, studies on other uses of silver nanoparticles acknowledged that
the particles act as antifungal agents in nail polishes and pigments in coloured
beauty products such as lipsticks and eye shadows. Despite the extensive use of
silver nanoparticles in the cosmetic line, there are still limited resources on the
mechanism of actions and the effect of the particles on the bio-functionality of
the body. The safety of silver nanoparticles could be comprehended from their skin
penetration ability and toxicity to the human body in which it could be justified
that both features are mainly influenced by the morphology of the particles and the
method of application. This article summarizes exclusively on the synthesis of
silver nanoparticles, the biomedical mechanisms and applications as well the
limitations with respect to skin penetration ability and toxicity effects which
will contribute significantly to the vast research on the association of
nanotechnology and cosmetics. (C) 2022 Published by Elsevier B.V. on behalf of King
Saud University.
AN - WOS:000798983400016
AU - Ong, W. T. J.
AU - Nyam, K. L.
C6 - MAR 2022
DA - APR
DO - 10.1016/j.sjbs.2022.01.035
IS - 4
PY - 2022
SN - 1319-562X
2213-7106
SP - 2085-2094
ST - Evaluation of silver nanoparticles in cosmeceutical and potential biosafety
complications
TI - Evaluation of silver nanoparticles in cosmeceutical and potential biosafety
complications
VL - 29
ID - 5879
ER -
TY - JOUR
AB - Purpose: To investigate the effects of melatonin on antioxidant capacity,
inflammation and apoptotic cell death (through expression of cleaved-caspase 3) in
lung tissue samples of diabetic rats. Methods: Thirty male Sprague-Dawley rats were
randomly divided into three groups. Group 1 (control group) was made up of healthy
rats. Group 2 (diabetes group) received streptozotocin at a dose of 50 mg/kg/day
for 5 days.Group 3 (diabetes plus melatonin group) received streptozotocin at a
dose of 50 mg/kg/day for 5 days and then they received melatonin at a dose of 20
mg/kg/day between 28thand 35thdays of the study. Results: Tissue MDA and MPO levels
were found to be significantly higher in diabetes group compared to control group
(p<0.05) whilst administration of melatonin was found to significantly lower this
increase down to normal levels (p<0.05). Bronchus associated lymphoid tissue (BALT)
was more severe in diabetics whereas administration of melatonin alleviated this
hyperplasia. Cleaved caspase 3 activity was severe in hyperplastic BALT in diabetic
rats however in lowered down to moderate level when melatonin was administered.
Conclusion: The melatonin caused an increase in antioxidant capacity and decreased
the expression of cleaved-caspase 3.
AD - Onk, Didem
Erzincan University. Faculty of Medicine. Department of Anesthesiology and
Reanimation. TR
Onk, Oruç Alper
Reanimation. TR
Erol, Hüseyin Serkan
Reanimation. TR
Özkaraca, Mustafa
Reanimation. TR
Çomaklı
, Selim
Reanimation. TR
Ayazoğ
lu, Tülin Akarsu
Reanimation. TR
Kuyrukluyı
ldı
z, Ufuk
Reanimation. TR
Ünver, Süheyla
Reanimation. TR
AU - Onk, Didem
AU - Onk, Oruç Alper
AU - Erol, Hüseyin Serkan
AU - Özkaraca, Mustafa
AU - Çomakl
AU - Selim
AU - Ayazo
AU - lu, Tülin Akarsu
AU - Kuyrukluy
AU - ld
AU - z, Ufuk
AU - Ünver, Süheyla
C1 - 20180510
DA - 2018/04
DB - LILACS
DO - 10.1590/s0102-865020180040000009
IS - 4
KW - Lung
Melatonin
Rats.
LA - en
PY - 2018
SN - 0102-8650
SP - 375-385
ST - Effect of melatonin on antioxidant capacity, inflammation and apoptotic cell
death in lung tissue of diabetic rats
T2 - Acta cir. bras
TI - Effect of melatonin on antioxidant capacity, inflammation and apoptotic cell
death in lung tissue of diabetic rats
86502018000400375
VL - 33
ID - 4924
ER -
TY - CHAP
AB - Since the advances in nanotechnology of a few decades ago, numerous
nanoparticles (NPs) have been manufactured and used throughout the world. NPs have
varied technological applications in electronics, photonics, medical, garments,
sporting goods, agricultural products and clean energy. Because the amount of NPs
to which humans are exposed is likely increasing, the health effects of NP exposure
and their underlying mechanism are being investigated. In order to utilize NPs more
safely, the means by which their negative effects are reduced must be discovered.
Previous studies have reported that various types of NPs, such as carbon black,
carbon nanotubes, silver, nickel, silica, silicon, and various metal oxides induce
reactive oxygen species (ROS), which cause oxidative stress and cell damage, to all
life forms (plants to mammals) regardless of species. For example, when single-wall
carbon nanotubes are intratracheally administered to rats (0.2 and 2.0 mg/kg/day,
once every three days, a total of 14 instillations), ROS in the lung are increased
and the exacerbation of allergic asthma is induced. Intravenous injection of
single-wall carbon nanotube suspension (6.25 and 12.5 mg/kg/day for 9 consecutive
days) induces oxidative stress in the brain and decreases locomotor activity in
mice. These reports demonstrate that the generation of ROS is one of the most
important factors in the mechanism of NP health effects. These studies also showed
the possibility of protective effects of antioxidants such as vitamin C (ascorbic
acid) and vitamin E (tocopherol) on NP-induced oxidative stress. The depletion of
antioxidants exacerbated the inflammatory response to NP exposure. Because
antioxidants reduce the effects of NPs via ROS elimination, these studies will
contribute to the safe and practical use of NPs. Some evidence suggests that
oxidative stress may be a starting point for the main mechanism underlying the NP
toxicity. According to the hierarchical oxidative stress hypothesis, the lowest
level of oxidative stress is associated with cytoprotective responses, such as the
induction of antioxidant and detoxification enzymes. If the level of protection
fails, the oxidative stress will lead to proinflammatory effects. Further
escalation will trigger disturbances in mitochondrial function, resulting in
cellular apoptosis or necrosis. Both endogenous and dietary antioxidants can be
considered as a first line of defense against the generation of ROS due to NP
exposure. Accordingly, it is highly possible that antioxidants may act as a
protective agent against damage induced by various types of NPs through their
downregulation of the oxidative stress level. © 2015 Nova Science Publishers, Inc.
AU - Onoda, A.
AU - Umezawa, M.
AU - Takeda, K.
DB - Scopus
KW - Antioxidant
Ascorbic acid
Nanoparticle
Omega-3 polyunsaturated fatty acid
Oxidative stress
α-tocopherol
PY - 2015
SP - 197-210
ST - The potential protective effect of antioxidants on nanoparticle toxicity
T2 - PM2.5: Role of Oxidative Stress in Health Effects and Prevention Strategy
TI - The potential protective effect of antioxidants on nanoparticle toxicity
84956814695&partnerID=40&md5=46bc66952b2ebe7c2b20490da3565683
ID - 5638
ER -
TY - JOUR
AB - Background: With the increasing use of nanomaterials, the need for methods
and assays to examine their immunosafety is becoming urgent, in particular for
nanomaterials that are deliberately administered to human subjects (as in the case
of nanomedicines). To obtain reliable results, standardised in vitro
immunotoxicological tests should be used to determine the effects of engineered
nanoparticles on human immune responses. However, before assays can be
standardised, it is important that suitable methods are established and
validated.Results: In a collaborative work between European laboratories, existing
immunological and toxicological in vitro assays were tested and compared for their
suitability to test effects of nanoparticles on immune responses. The prototypical
nanoparticles used were metal (oxide) particles, either custom-generated by wet
synthesis or commercially available as powders. Several problems and challenges
were encountered during assay validation, ranging from particle agglomeration in
biological media and optical interference with assay systems, to chemical
immunotoxicity of solvents and contamination with endotoxin.Conclusion: The
problems that were encountered in the immunological assay systems used in this
study, such as chemical or endotoxin contamination and optical interference caused
by the dense material, significantly affected the data obtained. These problems
have to be solved to enable the development of reliable assays for the assessment
of nano-immunosafety. © 2011 Oostingh et al; licensee BioMed Central Ltd.
AU - Oostingh, G. J.
AU - Casals, E.
AU - Italiani, P.
AU - Colognato, R.
AU - Stritzinger, R.
AU - Ponti, J.
AU - Pfaller, T.
AU - Kohl, Y.
AU - Ooms, D.
AU - Favilli, F.
AU - Leppens, H.
AU - Lucchesi, D.
AU - Rossi, F.
AU - Nelissen, I.
AU - Thielecke, H.
AU - Puntes, V. F.
AU - Duschl, A.
AU - Boraschi, D.
C7 - 8
DB - Scopus
DO - 10.1186/1743-8977-8-8
KW - Animals
Biological Assay
Cells
Cells, Cultured
Humans
Immunologic Factors
Interleukin-8
Metal Nanoparticles
Promoter Regions, Genetic
Solvents
adenylate kinase
cerium oxide
cobalt oxide
cytokine
endotoxin
gold
iron oxide
metal
metal oxide
nanoparticle
RNA
silver
silver oxide
solvent
unclassified drug
biomaterial
immunologic factor
interleukin 8
metal nanoparticle
article
biocompatibility
cell activation
cell assay
cell damage
cell proliferation
cell strain CACO 2
cell viability
contamination
controlled study
cytokine production
enzyme assay
enzyme release
gene expression
genotoxicity
human
human cell
human cell culture
immune response
immunomodulation
immunotoxicity
in vitro study
inflammatory cell
monocyte
optics
powder
priority journal
promoter region
reliability
RNA extraction
safety
toxicity testing
validation study
animal
bioassay
cell
cell culture
chemistry
cytology
genetics
immunology
metabolism
methodology
reproducibility
standard
M3 - Article
PY - 2011
ST - Problems and challenges in the development and validation of human cell-based
assays to determine nanoparticle-induced immunomodulatory effects
TI - Problems and challenges in the development and validation of human cell-based
assays to determine nanoparticle-induced immunomodulatory effects
79651474507&doi=10.1186%2f1743-8977-8-
8&partnerID=40&md5=b0613732505a7d252457840c59ba2a67
VL - 8
ID - 5734
ER -
TY - JOUR
AB - Silver nanoparticles are of interest to be used as antimicrobial agents in
wound dressings and coatings in medical devices, but potential adverse effects have
been reported in the literature. The possible local inflammatory response to silver
nanoparticles and the role of cell death in determining these effects are largely
unknown. Effects of the mixture of silver nanoparticles of different sizes were
compared in in vitro assays for cytotoxicity, caspase-1 and caspase-9 activity and
bax expression. In all tested concentrations, silver nanoparticles were more toxic
for RAW 264.7 monocytes than for 291.03C keratinocytes and induced significant
caspase-1 activity and necrotic cell death. In keratinocytes, more significantly
than in macrophages, silver nanoparticles led to increase of caspase-9 activity and
apoptosis. These results indicate that effects of silver nanoparticles depend on
the type of exposed cells. In addition, the potency of silver nanoparticles to
induce necrosis and caspase-1 activity in monocytes indicates their possible
immunotoxic inflammatory potential.
AN - WOS:000304381100008
AU - Orlowski, P.
AU - Krzyzowska, M.
AU - Winnicka, A.
AU - Chwalibog, A.
AU - Sawosz, E.
IS - 2
PY - 2012
SN - 1426-3912
SP - 123-130
ST - Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to
induce inflammatory reactions
T2 - CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY
TI - Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to
VL - 37
ID - 5839
ER -
TY - JOUR
AB - Hydrolyzable tannins are known to exhibit diverse biological effects, which
can be used in combination with silver nanoparticles (AgNPs). In this study, we
tested toxic and inflammatory properties of tannic-acid modified 13, 33, 46. nm and
unmodified 10-65. nm AgNPs using murine 291.03C keratinocyte and RAW 264.7 monocyte
cell lines. Both cell lines exposed for 24. h to 1-10. μg/ml of 13. nm, 33. nm, 46.
nm and unmodified AgNPs showed dose-dependent toxicity and decreased cell
proliferation. Only small-sized AgNPs induced production of ROS by monocytes, but
not keratinocytes. Monocytes internalized large aggregates of 33, 46. nm and 10-65.
nm AgNPs in cytoplasmic vacuoles, whereas keratinocytes accumulated less particles.
AgNPs of 13. nm were localized ubiquitously within both cell types. The tested
AgNPs strongly down-regulated production of tumor necrosis factor-α (TNF-α) by
monocytes, whereas keratinocytes exposed to AgNPs showed an opposite effect.
Unmodified but not tannic acid-modified AgNPs increased production of the pro-
inflammatory MCP-1 by monocytes and keratinocytes. In summary, low inflammatory
potential and lack of ROS production by tannic-acid modified AgNPs sized above 30.
nm suggests that tannic acid modification of large silver nanoparticles may help to
increase AgNPs biosafety. © 2013 Elsevier Ltd.
AU - Orlowski, P.
AU - Krzyzowska, M.
AU - Zdanowski, R.
AU - Winnicka, A.
AU - Nowakowska, J.
AU - Stankiewicz, W.
AU - Tomaszewska, E.
AU - Celichowski, G.
AU - Grobelny, J.
DB - Scopus
DO - 10.1016/j.tiv.2013.05.010
IS - 6
KW - Keratinocytes
Monocytes
Tannic acid
Animals
Apoptosis
Caspase 9
Cell Line
Cytokines
Metal Nanoparticles
Mice
Necrosis
Silver
Tannins
Murinae
silver nanoparticle
tannin
291.03C cell
animal cell
article
biosafety
cell line
cell proliferation
cell type
cell vacuole
controlled study
cytokine production
cytotoxicity
exposure
in vitro study
inflammation
internalization
keratinocyte
monocyte
mouse
nonhuman
particle size
RAW264.7 cell
M3 - Article
PY - 2013
SP - 1798-1808
ST - Assessment of in vitro cellular responses of monocytes and keratinocytes to
tannic acid modified silver nanoparticles
TI - Assessment of in vitro cellular responses of monocytes and keratinocytes to
tannic acid modified silver nanoparticles
84879352153&doi=10.1016%2fj.tiv.2013.05.010&partnerID=40&md5=0a58a9979f21010ca52940
829b80de75
VL - 27
ID - 5735
ER -
TY - JOUR
AB - Hydrolyzable tannins are known to exhibit anti-inflammatory activity, which
can be used in combination with silver nanoparticles (AgNPs) for dermal uses. In
this study, we investigated the effects of tannic acid-modified 13, 33, 46 nm and
unmodified 10-65 nm AgNPs using the human-derived keratinocyte HaCaT and VK2-E6/E7
cell lines in the form of stationary and spheroids cultures. After exposition to
tannic acid-modified AgNPs, VK2-E6/E7 cells showed higher toxicity, increased
production of reactive oxygen species (ROS) and activity of JNK stress kinase,
while HaCaT cell line demonstrated less ROS production and activation of ERK
kinase. AgNPs internalization was detected both in the superficial and internal
layers of spheroids prepared from both cell lines. Tannic acid modified AgNPs sized
above 30 nm did not induce DNA breaks in comet assay performed in both cell lines.
Tannic acid-modified but not unmodified AgNPs down-regulated TNF-α and LPS-
triggered production of IL-8 in VK2-E6/E7 but not in HaCaT cells. In summary,
tannic acid-modified AgNPs sized above 30 nm show good toxicological profile both
in vitro and possess immunomodulatory properties useful for potential dermal
applications in humans. © 2016 Elsevier B.V.
AU - Orlowski, P.
AU - Soliwoda, K.
AU - Bien, K.
AU - Fruba, A.
AU - Gniadek, M.
AU - Labedz, O.
AU - Nowak, Z.
AU - Grobelny, J.
AU - Krzyzowska, M.
DB - Scopus
DO - 10.1016/j.tiv.2016.05.009
KW - Human keratinocytes
Spheroids
Tannic acid
Cell Line
Cell Survival
Comet Assay
Humans
Immunologic Factors
Interleukin-8
JNK Mitogen-Activated Protein Kinases
Keratinocytes
Lipopolysaccharides
Metal Nanoparticles
Silver
Tannins
interleukin 8
silver nanoparticle
tannin
IL8 protein, human
immunologic factor
lipopolysaccharide
metal nanoparticle
silver
tannin derivative
Article
cell proliferation
comet assay
controlled study
cytotoxicity
drug mechanism
enzyme activation
enzyme activity
female
human
human cell
human cell culture
immunomodulation
in vitro study
internalization
cell line
cell survival
chemistry
drug effects
keratinocyte
metabolism
M3 - Article
PY - 2016
SP - 43-54
ST - Toxicity of tannic acid-modified silver nanoparticles in keratinocytes:
Potential for immunomodulatory applications
TI - Toxicity of tannic acid-modified silver nanoparticles in keratinocytes:
Potential for immunomodulatory applications
84973917515&doi=10.1016%2fj.tiv.2016.05.009&partnerID=40&md5=f39eeeb6fe275f2da9b62b
ddb30aa0c0
VL - 35
ID - 5472
ER -
TY - JOUR
AB - The interaction between silver nanoparticles and herpesviruses is attracting
great interest due to their antiviral activity and possibility to use as
microbicides for oral and anogenital herpes. In this work, we demonstrate that
tannic acid modified silver nanoparticles sized 13 nm, 33 nm and 46 nm are capable
of reducing HSV-2 infectivity both in vitro and in vivo. The antiviral activity of
tannic acid modified silver nanoparticles was size-related, required direct
interaction and blocked virus attachment, penetration and further spread. All
tested tannic acid modified silver nanoparticles reduced both infection and
inflammatory reaction in the mouse model of HSV-2 infection when used at infection
or for a post-infection treatment. Smaller-sized nanoparticles induced production
of cytokines and chemokines important for anti-viral response. The corresponding
control buffers with tannic acid showed inferior antiviral effects in vitro and
were ineffective in blocking in vivo infection. Our results show that tannic acid
modified silver nanoparticles are good candidates for microbicides used in
treatment of herpesvirus infections.
AN - WOS:000341230400032
AU - Orlowski, P.
AU - Gniadek, M.
AU - Baska, P.
AU - Nowakowska, J.
AU - Sokolowska, J.
AU - Nowak, Z.
AU - Donten, M.
AU - Grobelny, J.
AU - Krzyzowska, M.
C7 - e104113
DA - AUG 12
DO - 10.1371/journal.pone.0104113
IS - 8
PY - 2014
SN - 1932-6203
ST - Tannic Acid Modified Silver Nanoparticles Show Antiviral Activity in Herpes
Simplex Virus Type 2 Infection
T2 - PLOS ONE
TI - Tannic Acid Modified Silver Nanoparticles Show Antiviral Activity in Herpes
Simplex Virus Type 2 Infection
VL - 9
ID - 6130
ER -
TY - JOUR
AB - Background: Polyphenols possess antioxidant, anti-inflammatory and
antimicrobial properties and have been used in the treatment of skin wounds and
burns. We previously showed that tannic acid-modified AgNPs sized >26 nm promote
wound healing, while tannic acidmodified AgNPs sized 13 nm can elicit strong local
inflammatory response. In this study, we tested bimetallic Au@AgNPs sized 30 nm
modified with selected flavonoid and nonflavonoid compounds for wound healing
applications. Methods: Bimetallic Au@AgNPs were obtained by growing an Ag layer on
AuNPs and further modified with selected polyphenols. After toxicity tests and in
vitro scratch assay in HaCaT cells, modified lymph node assay as well as the mouse
splint wound model were further used to access the wound healing potential of
selected non-toxic modifications. Results: Tannic acid, gallic acid, polydatin,
resveratrol, catechin, epicatechin, epigallocatechin, epicatechin gallate,
epigallocatechin gallate and procyanidin B2 used to modify Au@AgNPs exhibited good
toxicological profiles in HaCaT cells. Au@AgNPs modified with 15 μM tannic acid,
200 μM resveratrol, 200 μM epicatechin gallate, 1000 μM gallic acid and 200 μM
procyanidin B2 induced wound healing in vivo and did not lead to the local
irritation or inflammation. Tannic acid-modified Au@AgNPs induced epithelial-
tomesenchymal transition (EMT) – like re-epithelialization, while other polyphenol
modifications of Au@AgNPs acted through proliferation and wound closure.
Conclusion: Bimetallic Au@AgNPs can be used as a basis for modification with
selected polyphenols for topical uses. In addition, we have demonstrated that
particular polyphenols used to modify bimetallic nanoparticles may show different
effects upon different stages of wound healing. © 2020 Orlowski et al.
AU - Orlowski, P.
AU - Zmigrodzka, M.
AU - Ranoszek-Soliwoda, K.
AU - Pajak, B.
AU - Slonska, A.
AU - Cymerys, J.
AU - Grobelny, J.
AU - Krzyzowska, M.
DB - Scopus
DO - 10.2147/IJN.S252027
KW - Bimetallic nanoparticles
Mouse model
Polyphenols
Wound healing
Animals
Antioxidants
Biflavonoids
Catechin
Gold
Metal Nanoparticles
Mice
Proanthocyanidins
Silver
Tannins
Wound Healing
catechin
epicatechin
epicatechin gallate
epigallocatechin
flavonoid
gallic acid
gold nanoparticle
piceid
polyphenol
procyanidin
resveratrol
silver nanoparticle
tannin
antioxidant
biflavonoid
gallocatechin
gold
metal nanoparticle
proanthocyanidin
procyanidin B2
silver
tannin derivative
animal cell
animal experiment
animal model
animal tissue
Article
cell proliferation
cervical lymph node
controlled study
cytotoxicity test
drug efficacy
drug formulation
female
HaCat cell line
in vitro study
in vivo study
mouse
nonhuman
skin injury
wound closure
wound healing
wound healing assay
animal
chemistry
drug effect
M3 - Article
PY - 2020
SP - 4969-4990
ST - Polyphenol-conjugated bimetallic au@agnps for improved wound healing
TI - Polyphenol-conjugated bimetallic au@agnps for improved wound healing
85087984867&doi=10.2147%2fIJN.S252027&partnerID=40&md5=7c686d580359629bb7b3c94c5c07
5dec
VL - 15
ID - 5423
ER -
TY - JOUR
AB - BackgroundSilver ions from silver nanoparticles (AgNP) or AgNPs themselves
itself that are ingested from consumer health care products or indirectly from
absorbed food contact material can interact with the gastrointestinal tract (GIT).
The permeability of the GIT is strictly regulated to maintain barrier function and
proper nutrient absorption. The single layer intestinal epithelium adheres and
communicates actively to neighboring cells and the extracellular matrix through
different cell junctions. In the current study, we hypothesized that oral exposure
to AgNPs may alter the intestinal permeability and expression of genes controlling
cell junctions. Changes in cell junction gene expression in the ileum of male and
female rats administered different sizes of AgNP for 13-weeks were assessed using
qPCR.ResultsThe results of this study indicate that AgNPs have an altering effect
on cell junctions that are known to dictate intestinal permeability. mRNA
expression of genes representing tight junction (Cldn1, Cldn5, Cldn6, Cldn10 and
Pecam1), focal adhesion (Cav1, Cav2, and Itgb2), adherens junction (Pvrl1, Notch1,
and Notch2), and hemidesmosome (Dst) groups were upregulated significantly in
females treated with 10nm AgNP, while no change or downregulation of same genes was
detected in male animals. In addition, a higher concentration of pro-inflammatory
cytokine, TNF-, was noticed in AgNP-treated female animals as compared to
controls.ConclusionsThis study proposes that interaction of silver with GIT could
potentially initiate an inflammatory process that could lead to changes in the
gastrointestinal permeability and/or nutrient deficiencies in sex-specific manner.
Fully understanding the mechanistic consequences of oral AgNP exposure may lead to
stricter regulation for the commercial usage of AgNPs and/or improved clinical
therapy in the future.
AN - WOS:000468053400004
AU - Orr, S. E.
AU - Gokulan, K.
AU - Boudreau, M.
AU - Cerniglia, C. E.
AU - Khare, S.
C7 - 63
DA - MAY 13
DO - 10.1186/s12951-019-0499-6
PY - 2019
SN - 1477-3155
ST - Alteration in the mRNA expression of genes associated with gastrointestinal
permeability and ileal TNF- secretion due to the exposure of silver nanoparticles
in Sprague-Dawley rats
TI - Alteration in the mRNA expression of genes associated with gastrointestinal
permeability and ileal TNF- secretion due to the exposure of silver nanoparticles
VL - 17
ID - 6045
ER -
TY - JOUR
AB - Background: Silver ions from silver nanoparticles (AgNP) or AgNPs themselves
itself that are ingested from consumer health care products or indirectly from
absorbed food contact material can interact with the gastrointestinal tract (GIT).
The permeability of the GIT is strictly regulated to maintain barrier function and
proper nutrient absorption. The single layer intestinal epithelium adheres and
communicates actively to neighboring cells and the extracellular matrix through
different cell junctions. In the current study, we hypothesized that oral exposure
to AgNPs may alter the intestinal permeability and expression of genes controlling
cell junctions. Changes in cell junction gene expression in the ileum of male and
female rats administered different sizes of AgNP for 13-weeks were assessed using
qPCR. Results: The results of this study indicate that AgNPs have an altering
effect on cell junctions that are known to dictate intestinal permeability. mRNA
expression of genes representing tight junction (Cldn1, Cldn5, Cldn6, Cldn10 and
Pecam1), focal adhesion (Cav1, Cav2, and Itgb2), adherens junction (Pvrl1, Notch1,
and Notch2), and hemidesmosome (Dst) groups were upregulated significantly in
females treated with 10 nm AgNP, while no change or downregulation of same genes
was detected in male animals. In addition, a higher concentration of pro-
inflammatory cytokine, TNF-α, was noticed in AgNP-treated female animals as
compared to controls. Conclusions: This study proposes that interaction of silver
with GIT could potentially initiate an inflammatory process that could lead to
changes in the gastrointestinal permeability and/or nutrient deficiencies in sex-
specific manner. Fully understanding the mechanistic consequences of oral AgNP
exposure may lead to stricter regulation for the commercial usage of AgNPs and/or
improved clinical therapy in the future. © 2019 The Author(s).
AU - Orr, S. E.
AU - Gokulan, K.
AU - Boudreau, M.
AU - Cerniglia, C. E.
AU - Khare, S.
C7 - 63
DB - Scopus
DO - 10.1186/s12951-019-0499-6
IS - 1
KW - Claudin
Gastrointestinal toxicity
Intestinal permeability
Tight junctions
Animals
Bodily Secretions
Cytokines
Female
Gastrointestinal Absorption
Ileum
Intestinal Mucosa
Male
Metal Nanoparticles
Particle Size
Permeability
Rats
RNA, Messenger
Silver
Tight Junctions
Cell membranes
Cytology
Metal ions
Metal nanoparticles
Nutrients
caveolin 1
caveolin 2
claudin 1
claudin 5
claudin 6
messenger RNA
nectin 1
Notch1 receptor
Notch2 receptor
silver nanoparticle
cytokine
metal nanoparticle
silver
Food contact material
Gastrointestinal toxicities
Intestinal epithelium
Intestinal permeabilities
adherens junction
animal experiment
animal model
Article
Cav1 gene
Cav2 gene
cell junction
Cldn1 gene
Cldn10 gene
Cldn5 gene
Cldn6 gene
controlled study
cytokine release
desmosome
down regulation
Dst gene
female
gene
gene expression
gene function
gene identification
genetic association
intestine mucosa permeability
Itgb2 gene
male
nonhuman
Notch1 gene
Notch2 gene
Pecam1 gene
Pvrl1 gene
rat
sex difference
Sprague Dawley rat
toxicity testing
upregulation
animal
bodily secretions
chemistry
drug effect
gastrointestinal absorption
genetics
ileum
intestine mucosa
metabolism
particle size
permeability
tight junction
Gene expression
M3 - Article
PY - 2019
ST - Alteration in the mRNA expression of genes associated with gastrointestinal
permeability and ileal TNF-α secretion due to the exposure of silver nanoparticles
TI - Alteration in the mRNA expression of genes associated with gastrointestinal
permeability and ileal TNF-α secretion due to the exposure of silver nanoparticles
85065647423&doi=10.1186%2fs12951-019-0499-
6&partnerID=40&md5=14f74fb7405fc9479a7e58cc3c2556e7
VL - 17
ID - 5436
ER -
TY - JOUR
AB - This study investigated the effects of topical application of chitosan-capped
silver nanoparticles (Ch/AgNPs) on burn wound healing. The chitosan-capped silver
nanoparticles were synthesized in one step from the silver nitrate, sodium
borohydride, and chitosan and were characterized using transmission electron
microscopy, fourier transform infrared spectroscopy, and X-ray diffraction methods.
The antioxidant assay was performed to evaluate the scavenging rate. The effects of
Ch/AgNPs on burn wound healing was also evaluated by histopathological, molecular,
and biochemical evaluations after 7, 14 and 28 days of treatment in a rat model. In
comparison to the negative control and silver sulfadiazine groups, the Ch/AgNPs
treated wounds exhibited significantly lower inflammatory reaction as determined by
the reduced level of interleukin-1 beta (IL-1 beta) and neutrophil counts.
Treatment by Ch/AgNPs also significantly enhanced re-epithelialization, so that
complete epithelialization was achieved in the lesions of the animals of this
group, at the 7th day post-wounding. Rapid reepithelialization, improved
granulation tissue formation, reduced IL-1 beta expression, mild inflammation, and
increased transforming growth factor-beta 1 and basic fibroblast growth factor, at
7 days post-wounding, are convincing reasons to confirm this idea that Ch/AgNPs are
effective in speeding up the wound healing stages. Our histopathological findings
are in agreement with the molecular and biochemical results and strongly
demonstrate that Ch/AgNPs stimulate burn wound healing by decreasing the length of
repair phases. Therefore, on the basis of our findings, Ch/AgNPs can be a promising
candidate in stimulating wound repair and regeneration.
AN - WOS:000443264400010
AU - Oryan, A.
AU - Alemzadeh, E.
AU - Tashkhourian, J.
AU - Ana, S. F. N.
DA - NOV 15
DO - 10.1016/j.carbpol.2018.07.077
PY - 2018
SN - 0144-8617
1879-1344
SP - 82-92
ST - Topical delivery of chitosan-capped silver nanoparticles speeds up healing in
burn wounds: A preclinical study
TI - Topical delivery of chitosan-capped silver nanoparticles speeds up healing in
burn wounds: A preclinical study
VL - 200
ID - 6125
ER -
TY - JOUR
AB - Silver nanoparticles are of interest to be used as antimicrobial agents in
wound dressings and coatings in medical devices, but potential adverse effects have
been reported in the literature. The possible local inflammatory response to silver
nanoparticles and the role of cell death in determining these effects are largely
unknown. Effects of the mixture of silver nanoparticles of different sizes were
compared in in vitro assays for cytotoxicity, caspase-1 and caspase-9 activity and
bax expression. In all tested concentrations, silver nanoparticles were more toxic
for RAW 264.7 monocytes than for 291.03C keratinocytes and induced significant
caspase-1 activity and necrotic cell death. In keratinocytes, more significantly
than in macrophages, silver nanoparticles led to increase of caspase-9 activity and
apoptosis. These results indicate that effects of silver nanoparticles depend on
the type of exposed cells. In addition, the potency of silver nanoparticles to
induce necrosis and caspase-1 activity in monocytes indicates their possible
immunotoxic inflammatory potential.
AU - Orłowski, P.
AU - Krzyzowska, M.
AU - Winnicka, A.
AU - Chwalibóg, A.
AU - Sawosz, E.
DB - Scopus
IS - 2
KW - Apoptosis
Keratinocytes
Monocytes
Toxicity
antiinfective agent
caspase 9
protein Bax
silver nanoparticle
animal cell
apoptosis
article
cell structure
controlled study
drug cytotoxicity
drug potency
enzyme activity
immunotoxicity
in vitro study
inflammation
keratinocyte
macrophage
material coating
medical device
monocyte
mouse
nonhuman
particle size
protein expression
wound dressing
M3 - Article
PY - 2012
SP - 123-130
ST - Toxicity of silver nanoparticles in monocytes and keratinocytes: Potential to
T2 - Central-European Journal of Immunology
TI - Toxicity of silver nanoparticles in monocytes and keratinocytes: Potential to
84862273890&partnerID=40&md5=4de07f69f438530139cf00e4a45e4a26
VL - 37
ID - 5685
ER -
TY - JOUR
AB - Environmental research has been significantly enriched by genomic and
proteomic methodologies. Particularly in the atmospheric pollution studies, where a
variety of environmental hazards act simultaneously and frequently in close
interrelationship, only such integrative methods can provide a comprehensive
analysis. Nanoparticles are a heterogeneous class of particles, but their health
effects have also common aspects. The scope of the article is to present the
contribution of genomics and proteomics in extending the knowledge about the
biological effects of atmospheric nanoparticles in humans: how they interact with
genes and genes' expression, how they affect translation and cell proteome and how
they interfere with epigenetic mechanisms. Different methods, with advantages and
disadvantages, and main contribution are described. Most studies underline the
oxidative stress and the inflammatory mechanisms induced by nanoparticles at the
bronchial epithelium level and at the systemic level. Oxidative mechanism is
investigated with genomic technics and confirmed in proteomic studies and in
epigenetic ones. These techniques have higher sensitivity in identifying parameters
related to cytoxicity of different human cells than other biological measurements
and an increasing role in evaluating environmental management and interventions.
AN - WOS:000366335000003
AU - Otelea, M.
AU - Rascu, A.
DA - OCT
DO - 10.30638/eemj.2015.243
IS - 10
PY - 2015
SN - 1582-9596
1843-3707
SP - 2283-2291
ST - GENOMICS AND PROTEOMICS TECHNIQUES IN NANOPARTICLES STUDIES - NEW APPROACH IN
ENVIRONMENTAL RESEARCH
T2 - ENVIRONMENTAL ENGINEERING AND MANAGEMENT JOURNAL
TI - GENOMICS AND PROTEOMICS TECHNIQUES IN NANOPARTICLES STUDIES - NEW APPROACH IN
ENVIRONMENTAL RESEARCH
VL - 14
ID - 6829
ER -
TY - JOUR
AB - After a biomaterial is implanted in a bone defect area, the immune response
and bacterial infection affect the success of bone regeneration. In this study, we
describe the development of a promising therapeutic approach to accelerate bone
regeneration via combining osteoimmunomodulatory and antibacterial activities.
Herein, we fabricated a nanosilver/halloysite nanotubes/gelatin methacrylate
(nAg/HNTs/GelMA) hybrid hydrogel and evaluated its osteoimmunomodulatory and
antibacterial properties in vitro and in vivo. The nAg/HNTs/GelMA hybrid hydrogel
had good biocompatibility with human periodontal ligament stem cells (hPDLSCs) and
macrophages. Moreover, the nAg/HNTs/GelMA hybrid hydrogel modulated inflammatory
cytokines secreted by macrophages and enhanced the osteogenic differentiation of
hPDLSCs in an inflammatory environment. In addition, nAg/HNTs/GelMA hybrid hydrogel
inhibited the growth of Gram-positive and Gram-negative bacteria in vitro and in
vivo. Compared with HNTs/GelMA hydrogel, the nAg/HNTs/GelMA hybrid hydrogel better
modulated the osteoimmune microenvironment and eliminated bacterial infection.
Thus, this hybrid hydrogel combining osteoimmunomodulatory with antibacterial
activities is a promising biomaterial for bone regeneration in defect areas.
AN - WOS:000503381200179
AU - Ou, Q. M.
AU - Huang, K. Q.
AU - Fu, C. Q.
AU - Huang, C. L.
AU - Fang, Y. F.
AU - Gu, Z. P.
AU - Wu, J.
AU - Wang, Y.
C7 - 123019
DA - FEB 15
DO - 10.1016/j.cej.2019.123019
PY - 2020
SN - 1385-8947
1873-3212
ST - Nanosilver-incorporated halloysite nanotubes/gelatin methacrylate hybrid
hydrogel with osteoimmunomodulatory and antibacterial activity for bone
regeneration
TI - Nanosilver-incorporated halloysite nanotubes/gelatin methacrylate hybrid
hydrogel with osteoimmunomodulatory and antibacterial activity for bone
regeneration
VL - 382
ID - 6779
ER -
TY - JOUR
AB - Phytochemicals offer immense promise for sustainable development and
production of nanotechnology-enabled products. In the present study, Olax nana
Wall. ex Benth. (family: Olacaceae) aqueous extract was used as an effective
stabilizing agent to produce biogenic silver (ON-AgNPs) and gold nanoparticles (ON-
AuNPs), which were investigated for biocompatibility and prospective biomedical
applications (antibacterial, anticancer, antileishmanial, enzyme inhibition,
antinociceptive, and anti-inflammatory activities). Various characterization
techniques (XRD, FTIR, SEM, TEM, DLS, EDX, and SAED) revealed efficient
biosynthesis of ON-AgNPs (26 nm) and ON-AuNPs (47 nm). In the toxicological
assessment, ON-AgNPs and ON-AuNPs were found biocompatible towards human RBCs and
macrophages (IC50 > 200 μg/mL). In a concentration range of 62.5–2000 μg/mL, a
strong antibacterial effect was produced by ON-AgNPs against Staphylococcus
epidermidis (MIC = 7.14 μg/mL) and Escherichia coli (8.25 μg/mL), while ON-AuNPs
was only active against Staphylococcus aureus (9.14 μg/mL). At a concentration of
3.9–500 μg/mL, a dose-dependant inhibition of HepG2 cancer cells was produced by
ON-AgNPs (IC50 = 14.93 μg/mL) and ON-AuNPs (2.97 μg/mL). Both ON-AgNPs and ON-AuNPs
were found active against Leishmania tropica (KMH23) promastigotes (IC50 = 12.56
and 21.52 μg/mL) and amastigotes (17.44 and 42.20 μg/mL), respectively, after
exposure to a concentration range of 1–200 μg/mL for 72 h. Preferential enzyme
inhibition against urease and carbonic anhydrase II were noted for ON-AgNPs (39.23
and 8.89%) and ON-AuNPs (31.34 and 6.34%), respectively; however, these were found
inactive against xanthine oxidase at 0.2 mg/mL. In the in vivo antinociceptive
(acetic acid-induced abdominal constrictions) and anti-inflammatory (carrageenan-
induced paw edema) activities, ON-AgNPs and ON-AuNPs at doses of 40 and 80 mg/kg,
significantly attenuated the tonic nociception (P < 0.001) and ameliorated the
carrageenan-induced inflammation (P < 0.01, P < 0.001). The results of in vitro and
in vivo activities indicated that the biogenic nanoparticles can be used as
valuable theranostic agents for further exploration of diverse biomedical
applications. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
AU - Ovais, M.
AU - Khalil, A. T.
AU - Raza, A.
AU - Islam, N. U.
AU - Ayaz, M.
AU - Saravanan, M.
AU - Ali, M.
AU - Ahmad, I.
AU - Shahid, M.
AU - Shinwari, Z. K.
DB - Scopus
DO - 10.1007/s00253-018-8928-2
IS - 10
KW - Biocompatible nanoparticles
Biological potential of nanoparticles
Green nanotechnology
Green synthesis of nanoparticles
Nanoparticle drug delivery
Phyto-nanotechnology
Bacteria
Colloids
Erythrocytes
Gold
Humans
Macrophages
Metal Nanoparticles
Plant Extracts
Prospective Studies
Silver
Theranostic Nanomedicine
Escherichia coli
Leishmania tropica
Olacaceae
Olax
Biochemistry
Biocompatibility
Carbonic anhydrase
Enzyme inhibition
Gold compounds
Gold nanoparticles
Ionic liquids
Nanoparticles
Silver compounds
antiinfective agent
antileishmanial agent
carbonate dehydratase II
cytotoxic agent
diclofenac
enzyme inhibitor
gold nanoparticle
Olax nana extract
plant extract
silver nanoparticle
unclassified drug
urease
xanthine oxidase
biomaterial
gold
metal nanoparticle
silver
Biological potential
Green nanotechnologies
Green synthesis
Toxicological assessment
bacterium
biological production
cancer
colloid
dicotyledon
drug
enzyme activity
inhibition
nanoparticle
nanotechnology
parasite
amastigote
analgesic activity
animal experiment
animal model
antiprotozoal activity
Article
biocompatibility
cancer inhibition
controlled study
dose response
drug cytotoxicity
drug synthesis
enzyme inhibition
erythrocyte
Escherichia coli B
green chemistry
Hep-G2 cell line
human
human cell
IC50
in vitro study
in vivo study
macrophage
mouse
nociception
nonhuman
normal human
Olax nana
promastigote
Vibrio cholerae
X ray diffraction
chemistry
drug effect
procedures
prospective study
synthesis
toxicity
Drug delivery
M3 - Article
PY - 2018
SP - 4393-4408
ST - Multifunctional theranostic applications of biocompatible green-synthesized
colloidal nanoparticles
T2 - Applied Microbiology and Biotechnology
TI - Multifunctional theranostic applications of biocompatible green-synthesized
colloidal nanoparticles
85044539492&doi=10.1007%2fs00253-018-8928-
2&partnerID=40&md5=60268b3a389cec82b3881d424925c8c7
VL - 102
ID - 5528
ER -
TY - JOUR
AB - Background and Objectives: Ocular alkaline burn is a clinical emergency that
can cause permanent vision loss due to limbal stem cell deficiency and corneal
neovascularization (CNV). Although the basic pathogenetic mechanisms are considered
to be acute oxidative stress and corneal neovascularization triggered by
inflammation, the underlying intracellular mechanisms have not been clearly
elucidated. The aim of this study was to investigate the role of endoplasmic
reticulum (ER) stress on inflammation and neovascularization, and the effect of the
ER stress inhibitor salubrinal (SLB), as a novel treatment in a corneal alkaline
burn model in rats. Methods: Chemical burns were created by cautery for 4 s using a
rod coated with 75% silver nitrate and 25% potassium nitrate in the corneal center
for the corneal neovascularization (CNV) model. Twenty-eight Wistar albino rats
were divided into four groups: SHAM, CNV, CNV + SLB, and CNV + bevacizumab (BVC).
After the CNV model was applied to the right eye, a single subconjunctival dose
(0.05 mL) of 1 mg/kg salubrinal was injected into both eyes in the CNV + SLB group.
A total of 1.25 mg/mL of subconjunctival BVC was administered to the CNV + BVC
group. Fourteen days after experimental modeling and drug administration, half of
the globes were placed in liquid nitrogen and stored at −20 °C until biochemical
analysis. The remaining tissues were collected and fixed in 10% buffered formalin
for histopathological and immunohistochemical analysis. Three qualitative agents
from three different pathways were chosen: TNFR for inflammation, endothelial
nitric oxide synthase (e-NOS) for vascular endothelial growth factor (VEGF)-
mediated vascular permeability, and caspase-3 for cellular apoptosis. Results:
Significantly lower caspase-3 and eNOS levels were detected in the CNV + SLB and
CNV + BVC groups than in the CNV group. Additionally, histopathological evaluation
revealed a significant decrease in neovascularization, inflammatory cell
infiltration, and fibroblast activity in the CNV + SLB and CNV + BVC groups. The
endoplasmic reticulum stress inhibitor, salubrinal, administered to the treatment
group, attenuated apoptosis (caspase-3) and inflammation (e-NOS). In the control
group (left eyes of the SLB group), salubrinal did not have a toxic effect on the
healthy corneas. Conclusion: The ER stress pathway plays an important role in
angiogenesis after alkaline corneal burns, and treatment with SLB modulates this
pathway, reducing caspase-3 and eNOS levels. Further studies are needed to
understand the molecular mechanisms altered by SLB-mediated therapy. The fact that
more than one mechanism plays a role in the pathogenesis of CNV may require the use
of more than one molecule in treatment. SLB has the potential to affect multiple
steps in CNV pathogenesis, both in terms of reducing ER stress and regulating
cellular homeostasis by inhibiting the core event of integrated stress response
(ISR). Therefore, it can be used as a new treatment option and as a strengthening
agent for existing treatments. Although blockade of intracellular organelle stress
pathways has shown promising results in experimental studies, more in-depth
research is needed before it can be used in routine practice. To the best of our
knowledge, this study is the first to report the role of ER stress in corneal
injury. © 2023 by the authors.
AU - Ozge, G.
AU - Karaca, U.
AU - Savran, M.
AU - Usta, G.
AU - Gulle, K.
AU - Sevimli, M.
AU - Cankara, F. N.
AU - Asci, H.
C7 - 323
DB - Scopus
DO - 10.3390/medicina59020323
IS - 2
KW - apoptosis
corneal injury
eNOS
inflammation
Animals
Bevacizumab
Burns, Chemical
Caspase 3
Corneal Neovascularization
Inflammation
Nitric Oxide Synthase Type III
Rats
Rats, Wistar
bevacizumab
caspase 3
endothelial nitric oxide synthase
salubrinal
vasculotropin A
animal
chemical burn
complication
cornea neovascularization
disease model
pathology
rat
Wistar rat
M3 - Article
PY - 2023
ST - Salubrinal Ameliorates Inflammation and Neovascularization via the Caspase
3/Enos Signaling in an Alkaline-Induced Rat Corneal Neovascularization Model
T2 - Medicina (Lithuania)
TI - Salubrinal Ameliorates Inflammation and Neovascularization via the Caspase
3/Enos Signaling in an Alkaline-Induced Rat Corneal Neovascularization Model
85148641067&doi=10.3390%2fmedicina59020323&partnerID=40&md5=8ec6da61785a7b182cee62d
7372f134d
VL - 59
ID - 4974
ER -
TY - JOUR
AB - The effective control of microbial and metabolically derived biological
toxins which negatively impact physical health remains a key challenge for the 21st
century. 2-Dimensional graphene and MXene nanomaterials are relatively new
additions to the field of biomedical materials with superior external surface areas
suited to adsorptive remediation of biological toxins. However, relatively little
is known about their physiological interactions with biological systems and, to
date, no comparative biological studies have been done. This study compares
titanium carbide MXene (Ti3C2Tx) in multilayered and delaminated forms with
graphene variants to assess the impact of variable physical properties on cellular
inflammatory response to endotoxin stimulus. No significant impact on cell
metabolism or induction of inflammatory pathways leading to cell death was
observed. No significant increase in markers of blood cell activation and
haemolysis occurred. Whilst graphene nanoplatelets (GNP), graphene oxide (GO) and
Ti3C2Tx showed insignificant antibacterial activity towards Escherichia coli,
silver nanoparticle-modified GO (GO-Ag) induced bacterial cell death and at a lower
dose than silver nanoparticles. All nanomaterials significantly reduced bacterial
endotoxin induced THP-1 monocyte IL-8, IL-6 and TNF-α cytokine production by >99%,
>99% and >80% respectively, compared to control groups. This study suggests the
utility of these nanomaterials as adsorbents in blood contacting medical device
applications for removal of inflammatory cytokines linked to poor outcome in
patients with life-threatening infection. © The Royal Society of Chemistry.
AU - Ozulumba, T.
AU - Ingavle, G.
AU - Gogotsi, Y.
AU - Sandeman, S.
DB - Scopus
DO - 10.1039/d0bm01953d
IS - 5
KW - Graphite
Humans
Inflammation
Metal Nanoparticles
Silver
Titanium
Biomedical materials
Blood
Cell death
Escherichia coli
Graphene Nanoplatelets
Metabolism
Metal nanoparticles
Titanium carbide
caspase 3
deionized water
endotoxin
functional group
graphene
graphene oxide
interleukin 6
interleukin 8
mxene
octoxinol
silver nanoparticle
titanium carbide
unclassified drug
graphite
metal nanoparticle
silver
titanium
Bacterial endotoxins
Cytokine production
Life-threatening infections
Physiological interactions
Variable physical properties
adsorption
apoptosis
Article
bacterial cell
cell death
cell interaction
cell metabolism
cell viability
comparative study
controlled study
cytokine production
cytolysis
cytotoxicity
death
DNA fragmentation
fluorescence
hemolysis
hydrodynamics
inflammation
membrane damage
nonhuman
oxidation
oxidative stress
particle size
pH
priority journal
surface area
surface charge
surface property
THP-1 cell line
ultrasound
human
Graphene
M3 - Article
PY - 2021
SP - 1805-1815
ST - Moderating cellular inflammation using 2-dimensional titanium carbide MXene
and graphene variants
TI - Moderating cellular inflammation using 2-dimensional titanium carbide MXene
and graphene variants
85102476795&doi=10.1039%2fd0bm01953d&partnerID=40&md5=8f0ca4cccbe578ff1fc9924c4b062
482
VL - 9
ID - 5184
ER -
TY - JOUR
AB - The novel coronavirus disease (COVID-19) pandemic has grasped the entire
world due to its high rate of spread with serious public health concern. The
scientific community has applied all possible therapeutic strategies to defeat the
virus, still the situation is not in control. So, as a fresh approach, the
"phytonanoparticles" can be used as a powerful gadget against COVID-19 because it
can be formulated to perform directly concerning the infection, enhancing drug
delivery system or by the way of stimulating the immunity of the patient. The plant
extract bioactive can offer its antioxidant, anti-inflammatory, immunomodulatory
effect for prophylaxis and treatment of SARS-CoV-2. Selective drug targeting of
these plant compounds is needed for augmenting drug stability, solubility,
increasing drug half-lives in the blood and reducing adverse effects in non-target
organs. Green nano-based drugs use plant extract as a bioreduction and capping
agent at room temperature. The green nanoparticles can be aimed to decrease the
oxidative stress and systemic inflammation of COVID-19 with increased activity and
lesser toxicity to normal cells. This review work summarises the antiviral,
immunomodulatory, antiinflammatory potential of green nanoparticles biosynthesised
with plant derived molecules with advanced delivery systems which has a possibility
to act as efficient potential remedy against coronavirus. This review discusses the
scientific explorations of phytonanoparticles which can protect human lives from
the devastation of SARS-CoV-2 because of its enhanced anticoronavirus biological
activity.
AN - WOS:000688453000019
AU - Palai, S.
AU - Patra, R.
AU - Dehuri, M.
DA - JAN-JUN
DO - 10.21276/ap.covid19.2021.10.1.20
IS - 1
PY - 2021
SN - 2393-9885
2278-9839
SP - S222-S230
ST - Phytonanoparticles and COVID-19
T2 - ANNALS OF PHYTOMEDICINE-AN INTERNATIONAL JOURNAL
TI - Phytonanoparticles and COVID-19
VL - 10
ID - 6677
ER -
TY - JOUR
AB - Objective Dystrophin, the missing or defective protein in Duchenne muscular
dystrophy, is expressed not only in muscle cells but also in vascular endothelial
cells (ECs). In this study, we assessed the effects of dystrophin deficiency on the
angiogenic capacities of ECs. Approach and Results We isolated vascular ECs from
mdx mice, the murine equivalent of Duchenne muscular dystrophy in humans, and wild-
type controls, and we found that mdx-derived ECs have impaired angiogenic
properties, in terms of migration, proliferation, and tube formation. They also
undergo increased apoptosis in vitro compared with wild-type cells and have
increased senescence-associated -galactosidase activity. Mdx-derived ECs also
display reduced ability to support myoblast proliferation when cocultured with
satellite cell-derived primary myoblasts. These endothelial defects are mirrored by
systemic impairment of angiogenesis in vivo, both on induction of ischemia,
stimulation with growth factors in the corneal model and matrigel plug assays, and
tumor growth. We also found that dystrophin forms a complex with endothelial NO
synthase and caveolin-1 in ECs, and that NO production and cGMP formation are
compromised in ECs isolated from mdx mice. Interestingly, treatment with aspirin
enhances production of both cGMP and NO in dystrophic ECs, whereas low-dose aspirin
improves the dystrophic phenotype of mdx mice in vivo, in terms of resistance to
physical exercise, muscle fiber permeability, and capillary density. Conclusions
These findings demonstrate that impaired angiogenesis is a novel player and
potential therapeutic target in Duchenne muscular dystrophy.
AN - WOS:000329283900023
AU - Palladino, M.
AU - Gatto, I.
AU - Neri, V.
AU - Straino, S.
AU - Smith, R. C.
AU - Silver, M.
AU - Gaetani, E.
AU - Marcantoni, M.
AU - Giarretta, I.
AU - Stigliano, E.
AU - Capogrossi, M.
AU - Hlatky, L.
AU - Landolfi, R.
AU - Pola, R.
DA - DEC
DO - 10.1161/ATVBAHA.112.301172
IS - 12
PY - 2013
SN - 1079-5642
1524-4636
SP - 2867-2876
ST - Angiogenic Impairment of the Vascular Endothelium A Novel Mechanism and
Potential Therapeutic Target in Muscular Dystrophy
T2 - ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
TI - Angiogenic Impairment of the Vascular Endothelium A Novel Mechanism and
Potential Therapeutic Target in Muscular Dystrophy
VL - 33
ID - 6771
ER -
TY - JOUR
AB - As the knowledge about the interferences of nanomaterials on human staminal
cells are scarce and contradictory, we undertook a comparative multidisciplinary
study based on the size effect of zero-valent iron, cobalt, and nickel
microparticles (MPs) and nanoparticles (NPs) using human adipose stem cells (hASCs)
as a model, and evaluating cytotoxicity, morphology, cellular uptake, and gene
expression. Our results suggested that the medium did not influence the cell
sensitivity but, surprisingly, the iron microparticles (FeMPs) resulted in being
toxic. These data were supported by modifications in mRNA expression of some genes
implicated in the inflammatory response. Microscopic analysis confirmed that NPs,
mainly internalized by endocytosis, persist in the vesicles without any apparent
cell damage. Conversely, MPs are not internalized, and the effects on hASCs have to
be ascribed to the release of ions in the culture medium, or to the reduced oxygen
and nutrient exchange efficiency due to the presence of MP agglomerating around the
cells. Notwithstanding the results depicting a heterogeneous scene that does not
allow drawing a general conclusion, this work reiterates the importance of
comparative investigations on MPs, NPs, and corresponding ions, and the need to
continue the thorough verification of NP and MP innocuousness to ensure unaffected
stem cell physiology and differentiation.
AN - WOS:000408759500018
AU - Palombella, S.
AU - Pirrone, C.
AU - Rossi, F.
AU - Armenia, I.
AU - Cherubino, M.
AU - Valdatta, L.
AU - Raspanti, M.
AU - Bernardini, G.
AU - Gornati, R.
C7 - 212
DA - AUG
DO - 10.3390/nano7080212
IS - 8
PY - 2017
SN - 2079-4991
ST - Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model
T2 - NANOMATERIALS
TI - Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model
VL - 7
ID - 6491
ER -
TY - JOUR
AB - Slow wound healing caused by bacterial infection is a critical clinical
challenge. Moreover, finding common preservatives that fulfill current medical
requirements has proved difficult. Therefore, we designed a multi-functional wound
dressing based on the hydrogen-bonded assembly of polyvinyl alcohol (PVA)/polyhexa-
methylene biguanide (PHMB)/platelet-rich plasma (PRP). The hydrogels were prepared
by a simple and environmentally friendly procedure by repeated freeze-thaw cycles
without a chemical crosslinking agent. Characterization studies revealed the
outstanding stability of these composite hydrogels. Bacteriostatic ring experiments
revealed that the PHMB-containing hydrogel possessed strong antibacterial effects.
The hydrogel with PRP showed low cytotoxicity and significantly stimulated the
proliferation of L929 fibroblasts, according to live/dead cell staining and 3-(4,5-
dimethylthiazol-2-yl)- 5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2 H-
tetrazolium (MTS) testing results. The PVA/PHMB/PRP composite dressing reduced
inflammatory response, promoted epithelization, enhanced collagen production and
vascular regeneration, and restored skin appendages in vivo. These results indicate
that the PVA/PHMB/PRP wound dressing is a promising biological material with
synergistic antibacterial and wound-healing functions.
AN - WOS:000884810200001
AU - Pan, L. F.
AU - Li, C. H.
AU - Wang, Z. C.
AU - Yang, L. H.
AU - Zhang, L. B.
C6 - OCT 2022
C7 - 108626
DA - NOV
DO - 10.1016/j.bej.2022.108626
PY - 2022
SN - 1369-703X
1873-295X
ST - Preparation of an antibacterial dressing for simultaneous delivery of
polyhexamethylene biguanide and platelet-rich plasma, and evaluation of the
dressing's ability to promote infected skin repair
T2 - BIOCHEMICAL ENGINEERING JOURNAL
TI - Preparation of an antibacterial dressing for simultaneous delivery of
polyhexamethylene biguanide and platelet-rich plasma, and evaluation of the
dressing's ability to promote infected skin repair
VL - 187
ID - 6792
ER -
TY - JOUR
AB - Objective: To study the dynamic expression and distribution of high mobility
group box 1 (HMGB-1) in diffuse axonal injury (DAI) in rats and to clarify its
involvement in the inflammatory reaction after DAI in rats, in order to provide new
targets for the clinical treatment of DAI. Methods: A DAI model was established
using a coronal rotation device and evaluated by HE, Glees-Marsland silver
staining, and Mallory phosphotungstic acid hematoxylin staining.
Immunohistochemistry, Western blot and RT-PCR were used to detect the expression
and distribution of HMGB-1 in the cortex of DAI rats at 6 h, 1 d, 3 d and 7 d. And
TUNEL was used to examine the apoptosis of neurons in DAI rats. Results:
Immunohistochemical results showed that at 6 h and 1 d after DAI, the number of
HMGB-1-positive cells decreased, but at 3 and 7 d it began to increase. Western
blot also showed that during the early stage after DAI (6 h and 1 d), the level of
HMGB-1 protein in the cortex was significantly lower than that in the control
group, but at the late stage (3 and 7 d) after DAI it significantly increased
compared with that in the control group until 7 d. RT-PCR showed that at 6 h after
DAI there was no significant increase in the level of HMGB-1mRNA, but at 1 d there
was a slight increase compared with the control group; at 3 and 7 d, it showed an
obvious significance. TUNEL staining indicated that the significant neuronal
apoptosis appeared as early as 6 h after DAI, and reached the peak at 3 d; it
started to decrease at 7 d but still remained at a relatively high level.
Conclusion: The dynamic expression and distribution of HMGB-1 showed significant
changes with the time course after DAI in rats. They decreased at the early stage
but increased at the late stage. At the early stage, HMGB-1 is mainly passively
released by the necrotic neurons, and at the late stage it may be actively secreted
by the active inflammatory cells. HMGB-1 may mediate the post-DAI neural cell
apoptosis by inducing the inflammatory reaction. ©, 2015, Journal of Xi'an Jiaotong
University (Medical Sciences). All right reserved.
AU - Pang, H. G.
AU - Song, J. N.
AU - Li, D. D.
AU - Sun, P.
AU - Zhao, Y. L.
AU - Huang, T. Q.
AU - Zhai, H. C.
AU - An, J. Y.
DB - Scopus
DO - 10.7652/jdyxb201503004
IS - 3
KW - Apoptosis
Diffuse axonal injury (DAI)
High mobility group box 1
Traumatic brain injury
eosin
hematoxylin
high mobility group protein
messenger RNA
phosphotungstic acid
animal experiment
animal model
animal tissue
apoptosis
Article
axonal injury
brain cortex
control group
controlled study
disease course
nerve cell
nonhuman
protein expression
rat
staining
Western blotting
M3 - Article
PY - 2015
SP - 304-309
ST - Dynamic expression and distribution of high mobility group box 1 in diffuse
T2 - Journal of Xi'an Jiaotong University (Medical Sciences)
TI - Dynamic expression and distribution of high mobility group box 1 in diffuse
84929618205&doi=10.7652%2fjdyxb201503004&partnerID=40&md5=cf41dbe8670d87a46573ccb3b
5279292
VL - 36
ID - 5629
ER -
TY - JOUR
AB - Isohexenylnaphthazarins (IHN), commonly known as Alkannins and Shikonins
(A/S), are lipophilic red pigments. They are found in the outer surface of the
roots of at least a hundred and fifty species that belong to the genera Alkanna,
Lithospermum, Echium, Onosma, Anchusa and Cynoglossum of the Boraginaceae family.
The chiral pairs A/S are potent pharmaceutical substances with a well-established
and wide spectrum of wound healing, antimicrobial, anti-inflammatory, antioxidant,
anticancer and antithrombotic biological activity. For organic chemists uninitiated
in the chemistry of quinones, the structures of alkannin (1) and shikonin (2) may
look misleading simple. However, in spite of great efforts over many years by
several research groups worldwide, a much needed viable synthetic route to these
enantiomers has remained elusive until very recently. The value of A/S motivated
biotechnologists to develop the world's first manufacturing process utilizing plant
cell cultures. The research in this area has provided a wealth of knowledge to the
field of biotechnology. In addition, great insights into the biosynthesis of these
natural products and to our understanding of plant secondary metabolism in general,
has been gained from this work. The last years there has been extensive scientific
research in many areas throughout the disciplines of chemistry and biology and more
specifically in cancer chemotherapy and a number of papers have appeared in the
literature. Significant research has been conducted on A/S effectiveness on several
tumors and on their mechanism of anticancer action. The aim of this paper was to
review the recent advances in chemistry, biology, biotechnology and biosynthesis of
alkannins and shikonins. © 2006 Bentham Science Publishers Ltd.
AU - Papageorgiou, V. P.
AU - Assimopoulou, A. N.
AU - Samanidou, V. F.
AU - Papadoyannis, I. N.
DB - Scopus
DO - 10.2174/138527206778742704
IS - 16
KW - Alkannins Biosynthesis
Antimicrobial
Antitumor
Bacterial gene ubiC
DNA Topoisomerases
Shikonin derivatives
(3 hydroxyisovaleryl)shikonin
acetylalkannin
acetylshikonin
alkannin
angelylalkannin
angelylshikonin
anhydroalkannin
arnebin 1
arnebin 2
arnebin 3
arnebin 4
arnebin 5
arnebin 6
cycloarnebin 7
deoxyshikonin
fluconazole
haloacetylshikonin derivative
hydrocortisone
isobutylalkannin
isobutylshikonin
meticillin
olive oil
plant extract
povidone iodine
propionylshikonin
shikonin derivative
sulfadiazine silver
teracrylalkannin
unclassified drug
unindexed drug
vancomycin
apoptosis
arthritis
bacterial strain
biological activity
biotechnology
cancer inhibition
drug effect
drug efficacy
drug indication
drug isolation
drug mechanism
drug research
drug structure
enantioselectivity
enzyme inhibition
epithelization
Escherichia coli
human
molecular biology
nonhuman
oligomerization
plant cell culture
polymerization
review
wound healing
M3 - Review
PY - 2006
SP - 2123-2142
ST - Recent advances in chemistry, biology and biotechnology of Alkannins and
Shikonins
T2 - Current Organic Chemistry
TI - Recent advances in chemistry, biology and biotechnology of Alkannins and
Shikonins
33750556630&doi=10.2174%2f138527206778742704&partnerID=40&md5=c69a1bb8c935104885841
fb5eec9f19b
VL - 10
ID - 5831
ER -
TY - JOUR
AB - Tendon injuries are commonly met in the emergency department. Unfortunately,
tendon tissue has limited regeneration potential and usually the consequent
formation of scar tissue causes inferior mechanical properties. Nanoparticles could
be used in different way to improve tendon healing and regeneration, ranging from
scaffolds manufacturing (increasing the strength and endurance or anti-adhesions,
anti-microbial, and anti-inflammatory properties) to gene therapy. This paper aims
to summarize the most relevant studies showing the potential application of
nanoparticles for tendon tissue regeneration. © 2016 Parchi, Vittorio, Andreani,
Battistini, Piolanti, Marchetti, Poggetti and Lisanti.
AU - Parchi, P. D.
AU - Vittorio, O.
AU - Andreani, L.
AU - Battistini, P.
AU - Piolanti, N.
AU - Marchetti, S.
AU - Poggetti, A.
AU - Lisanti, M.
C7 - 202
DB - Scopus
DO - 10.3389/fnagi.2016.00202
IS - AUG
KW - Gold nanoparticles
Nanoparticles
Scaffold
Tendon injuries
cellulose
chitosan
dextran
gold nanoparticle
ibuprofen
mesoporous silica nanoparticle
microRNA
nanocrystal
nanoparticle
polyglactin
polylactide
silver nanoparticle
achilles tendon
antiadhesion activity
bacterium adherence
biocompatibility
biotechnological production
cell labeling
cell viability
drug activity
drug release
fiber
human
nanotechnology
nonhuman
nonviral gene delivery system
Short Survey
stem cell
tendon
tendon injury
tissue engineering
tissue regeneration
M3 - Short survey
PY - 2016
ST - Nanoparticles for tendon healing and regeneration: Literature review
T2 - Frontiers in Aging Neuroscience
TI - Nanoparticles for tendon healing and regeneration: Literature review
84990062648&doi=10.3389%2ffnagi.2016.00202&partnerID=40&md5=476c4a06443ddf704705d64
ee34f5ec0
VL - 8
ID - 5571
ER -
TY - JOUR
AB - Natural bioflavonoids are an essential component of dietary supplements
possessing antimicrobial properties. Many of the bioflavonoids have resulted in
positive antitumor, anticancer, antibacterial, antifungal, anti-inflammatory
properties, but the efficacy remains low due to toxicity at the molecular level
whereas antiviral property limits to negative. The synergistic link between
nanoscience and flavonoid chemistry enhances the epidemiological properties of
flavonoid and also diminish the antimicrobial resistivity (AMR) by forming their
hybrid nanocomposites. Nanochemistry uses various nanocomposite and nano materials
for biosensing the flavonoids and their delivery as a drug. The quercetin flavonoid
and its derivatives such as rutin, and myricetin are used for sensing and drug
delivery. Quercetin with 15Carbon-5Hydroxyl chemical scaffold has been explored for
a few decades for the development of hybrid nanocomposite and nanomaterial with
metallic as well as organic nano co-composites. This quercetin flavonoid based
hybrid nanocomposites seemed to show a significant effect on In vitro and some
animal model processes along with attenuating lipid peroxidation, platelet
aggregation, and capillary permeability actions. This review mainly focused on the
hybrid nanoscience of quercetin bioflavonoid and its application in numerous
biological, material fields with a future perspective. (c) 2020 Published by
Elsevier B.V. on behalf of King Saud University. This is an open access article
under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
AN - WOS:000605507200022
AU - Parhi, B.
AU - Bharatiya, D.
AU - Swain, S. K.
DA - DEC
DO - 10.1016/j.jsps.2020.10.017
IS - 12
PY - 2020
SN - 1319-0164
2213-7475
SP - 1719-1732
ST - Application of quercetin flavonoid based hybrid nanocomposites: A review
T2 - SAUDI PHARMACEUTICAL JOURNAL
TI - Application of quercetin flavonoid based hybrid nanocomposites: A review
VL - 28
ID - 6753
ER -
TY - JOUR
AB - Little attention has been paid to the toxicity of silver amalgam fillings,
which have been used over the centuries in Dentistry. Amalgam particles may
accidentally and/or traumatically be embedded into the submucosal tissue during
placement of a restoration and perpetuate in such area. This article presents a
case of amalgam tattoo and investigates whether it is related to the patient's
repeated episodes of sinusitis. The patient was a 46-year-old woman with a 2 mm
diameter radiopaque lesion in the right oral mucosa detected on a panoramic
radiograph and presented as a black macula clinically. A complete surgical
resection was carried out. The histopathological examination revealed deposits of
dark-brownish pigments lining the submucosal tissue with adjacent lymphocytic
inflammatory infiltrate and multinucleated giant cells phagocyting pigments. There
was a negative staining for both iron and melanin. One year after lesion removal,
the patient reported that the sinusitis crises had ceased after repeated episodes
for years. It may be speculated that the inflammatory process related to amalgam
tattoo seems to lead to a local immune response that causes sinusitis because it
enhances the human leukocyte antigen DR (HLA-DR) tissue expression.
AU - Parizi, J. L. S.
AU - Nai, G. A.
DB - Scopus
DO - 10.1590/S1678-77572010000100016
IS - 1
KW - Amalgam
Dental restorative material
HLA-DR
Sinusitis
Tattoo
M3 - Article
PY - 2010
SP - 100-104
ST - Amalgam tattoo: A cause of sinusitis?
T2 - Journal of Applied Oral Science
TI - Amalgam tattoo: A cause of sinusitis?
77950887646&doi=10.1590%2fS1678-
77572010000100016&partnerID=40&md5=93bbdbacc6d7ab2c8c09334366194090
VL - 18
ID - 5748
ER -
TY - JOUR
AB - Little attention has been paid to the toxicity of silver amalgam fillings,
which have been used over the centuries in Dentistry. Amalgam particles may
accidentally and/or traumatically be embedded into the submucosal tissue during
placement of a restoration and perpetuate in such area. This article presents a
case of amalgam tattoo and investigates whether it is related to the patient's
repeated episodes of sinusitis. The patient was a 46-year-old woman with a 2 mm
diameter radiopaque lesion in the right oral mucosa detected on a panoramic
radiograph and presented as a black macula clinically. A complete surgical
resection was carried out. The histopathological examination revealed deposits of
dark-brownish pigments lining the submucosal tissue with adjacent lymphocytic
inflammatory infiltrate and multinucleated giant cells phagocyting pigments. There
was a negative staining for both iron and melanin. One year after lesion removal,
the patient reported that the sinusitis crises had ceased after repeated episodes
for years. It may be speculated that the inflammatory process related to amalgam
tattoo seems to lead to a local immune response that causes sinusitis because it
enhances the human leukocyte antigen DR (HLA-DR) tissue expression.
AD - Parizi, José Luiz Santos
University of Western São Paulo. Department of Pathology. Presidente Prudente. BR
Nai, Gisele Alborghetti
University of Western São Paulo. Department of Pathology. Presidente Prudente. BR
AU - Parizi, José Luiz Santos
AU - Nai, Gisele Alborghetti
C1 - 20100504
DA - 2010/02
DB - LILACS
IS - 1
KW - Amalgam
Dental restorative material
HLA-DR
Sinusitis
Tattoo
LA - en
PY - 2010
SN - 1678-7757
SP - 100-104
ST - Amalgam tattoo: a cause of sinusitis?
TI - Amalgam tattoo: a cause of sinusitis?
77572010000100016
VL - 18
ID - 4949
ER -
TY - JOUR
AB - Objectives/Hypothesis: To determine the resorption rate and biocompatibility
characteristics of novel cross-linked hydrogel ventilation tubes and varied
formulations of polyester ventilation tubes in a Chinchilla model. Study Design:
Animal Study. Methods: Three cross-linked glycosaminoglycan hydrogel ventilation
tubes fabricated by cross-linking thiol-modified chondroitin sulfate or thiol-
modified carboxymethylated hyaluronic acid, four different polyester ventilation
tubes (poly L-lactide [PLA], 50/50 poly D,L-lactide-co-glycolide [PLGA], and
silver-impregnated versions of PLA and PLGA tubes) were placed into the tympanic
membranes of chinchillas. Commercially available fluoroplastic ventilation tubes
were placed in the contralateral ear of each animal to serve as a control.
Integrity of the tubes was assessed by weekly otoscopy. Biocompatibility was
assessed by auditory brainstem response, by otoscopic and histologic examination of
the tympanic membrane at the tube site. Results: The hydrogel tubes had very short
resorption times that expanded and enlarged the myringotomy site. PLGA and silver-
coated PLGA tubes maintained their integrity in the tympanic membrane for similar
durations of 18.9 ± 6.4 days and 21.0 ± 6.0 days, respectively. The silver-coated
PLGA tubes had lower neutrophil and fibrosis scores than PLGA tubes. PLA tubes
demonstrated equivalent findings to commercially available nonresorbable tubes with
respect to otoscopic findings, auditory brainstem response thresholds, and
histologic inflammatory scores. Conclusions: Resorbable polyester pressure
equalization tubes demonstrate predictable resorption behavior and similar
biocompatibility characteristics when compared with nonresorbable tubes. Silver
modification may confer some stability to PLGA tubes. Hydrogel tubes have very
short resorption times, tend to enlarge the myringotomy site, and show greater
inflammatory changes. Copyright © 2013 The American Laryngological, Rhinological,
and Otological Society, Inc.
AU - Park, A. H.
AU - Hoyt, D.
AU - Britt, D.
AU - Chase, S.
AU - Tansavatdi, K.
AU - Hunter, L.
AU - McGill, L.
AU - Sheng, X.
AU - Skardal, A.
AU - Prestwich, G. D.
DB - Scopus
DO - 10.1002/lary.23712
IS - 4
KW - chinchilla
hyaluronic acid
polyester
Resorbable ear ventilation tubes
Absorbable Implants
Animals
Chinchilla
Hydrogel
Middle Ear Ventilation
Models, Animal
Polyesters
Tympanic Membrane
chondroitin sulfate
cross linked glycosaminoglycan hydrogel
glycosaminoglycan
polyglactin
polylactide
thiol
unclassified drug
analytical parameters
animal experiment
animal tissue
article
auditory brainstem response threshold
biocompatibility
controlled study
eardrum
fibrosis
histologic inflammatory score
histopathology
medical parameters
neutrophil count
nonhuman
otoscopy
pressure equalization tube
priority journal
resorption rate
sensory system parameters
silver impregnation
tube
M3 - Article
PY - 2013
SP - 1043-1048
ST - Cross-linked hydrogel and polyester resorbable ventilation tubes in a
Chinchilla model
T2 - Laryngoscope
TI - Cross-linked hydrogel and polyester resorbable ventilation tubes in a
Chinchilla model
84875459066&doi=10.1002%2flary.23712&partnerID=40&md5=4351b5e9357444641139b16bda0bc
2f3
VL - 123
ID - 5738
ER -
TY - JOUR
AB - Toxicity of nanoparticles depends on many factors including size, shape,
chemical composition, surface area, surface charge, and others. In this study, we
compared the toxicity of different sized-silver nanoparticles (AgNPs) which are
being widely used in consumer products due to its unique antimicrobial activity.
When mice were treated with AgNPs 1 mg/kg for 14 days by oral administration,
small-sized AgNPs (22 nm, 42 nm, and 71 nm) were distributed to the organs
including brain, lung, liver, kidney, and testis while large-sized AgNPs (323. nm)
were not detected in those tissues. The levels of TGF-β in serum were also
significantly increased in the treated group of small-sized AgNPs but not in large-
sized AgNPs. In addition, B cell distribution was increased in small-sized AgNPs
but not in large-sized-AgNPs by the phenotype analysis. However, body weight or in
the ratio of organ/body weight were not different between the control group and all
the AgNPs-treated groups. The repeated-dose toxicity of AgNPs (42. nm) was also
investigated in mice by oral administration for 28 days. By the administration of
AgNPs (0.25. mg/kg, 0.50. mg/kg, 1.00. mg/kg), adverse impacts on liver and kidney
were observed in a high dose-treated group (1.00. mg/kg), when determined by blood
chemistry and histipathological analysis. Cytokines including IL-1, IL-6, IL-4, IL-
10, IL-12, and TGF-β were also increased in a dose-dependent manner by repeated
oral administration. In addition, B cell distribution in lymphocyte and IgE
production were increased. Based on these results, it is suggested that repeated
oral administration of nano-sized AgNPs may cause organ toxicity and inflammatory
responses in mice. © 2010 Elsevier B.V.
AU - Park, E. J.
AU - Bae, E.
AU - Yi, J.
AU - Kim, Y.
AU - Choi, K.
AU - Lee, S. H.
AU - Yoon, J.
AU - Lee, B. C.
AU - Park, K.
DB - Scopus
DO - 10.1016/j.etap.2010.05.004
IS - 2
KW - Inflammation
Repeated-dose toxicity
Silver nanoparticle
Size differences
Mus
creatinine
immunoglobulin E
interleukin 1
interleukin 10
interleukin 12
interleukin 4
interleukin 6
silver nanoparticle
animal cell
animal experiment
animal tissue
antibody production
article
B lymphocyte
body weight
brain
CD4 CD8 ratio
controlled study
cytokine production
female
histopathology
immunophenotyping
inflammation
kidney
liver
lung
lymphocyte
male
mouse
natural killer cell
nonhuman
organ weight
particle size
priority journal
testis
tissue distribution
toxicity testing
weight change
M3 - Article
PY - 2010
SP - 162-168
ST - Repeated-dose toxicity and inflammatory responses in mice by oral
administration of silver nanoparticles
TI - Repeated-dose toxicity and inflammatory responses in mice by oral
administration of silver nanoparticles
77955419251&doi=10.1016%2fj.etap.2010.05.004&partnerID=40&md5=2d92ba2ccc9b11e6d1f0c
e4f21d2e26c
VL - 30
ID - 5721
ER -
TY - JOUR
AB - The adverse effects of silver nanoparticles (AgNPs) to human and environment
have not been known well, although AgNPs are now widely applicated to consumer
products. In this study, we investigated the inflammatory responses including
cytokine production and gene expression in mice after a single intratracheal
instillation of AgNPs. As results, pro-inflammatory cytokines (IL-1, TNF-a, and IL-
6) and Th0 cytokine (IL-2) were progressively increased by the day 28 after a
single instillation. The secretion of Th2 type cytokine was more dominant than that
of Th1 type cytokine, and the increase of B cell distribution was also observed.
But, histopathological changes in lung were observed only at day 1 after
instillation. We identified the changes of gene expression induced by AgNPs in lung
tissue using microarray. The 261 genes related to inflammation and tissue damages
including Saa3, Krt 13, Lor, Krtdap, and Lcn 2 were up-regulated by over 2 fold,
while 103 genes including H2-Ea, Chka, BC030476, Heg1 and Hbb-b1 were down-
regulated. Based on the data, it is suggested that AgNPs may induce Th2 type
dominant inflammatory responses and tissue damage in the lung of mice.
AN - WOS:000287949000017
AU - Park, E. J.
AU - Choi, K.
AU - Park, K.
DA - FEB
DO - 10.1007/s12272-011-0216-y
IS - 2
PY - 2011
SN - 0253-6269
1976-3786
SP - 299-307
ST - Induction of Inflammatory Responses and Gene Expression by Intratracheal
Instillation of Silver Nanoparticles in Mice
T2 - ARCHIVES OF PHARMACAL RESEARCH
TI - Induction of Inflammatory Responses and Gene Expression by Intratracheal
Instillation of Silver Nanoparticles in Mice
VL - 34
ID - 5908
ER -
TY - JOUR
AB - In this study, we identified the toxic effects of sheet-type titania (TNS),
which are being developed as a material for UV-blocking glass, comparing with P25,
a benchmark control for titania, in MH-S cells, a mouse alveolar macrophage cell
line. After 24 h exposure, the TNS-exposed cells formed large vacuoles while the
P25-exposed ones did not. The decreased levels of cell viability were similar
between the P25 and TNS groups, but ATP production was clearly lower in cells
exposed to the TNS. P25 decreased the expression of calnexin protein, an
endoplasmic reticulum (ER) membrane marker, and increased the number of cells
generating ROS in a dose dependent manner. Meanwhile, TNS dilated the ER and
mitochondria and increased the secretion of NO and pro-inflammatory cytokines, but
not of ROS. Subsequently, we studied the molecular response following TNS-induced
vacuolization. TNS started to form vacuoles in the cytosol since 20 min after
exposure, and the expression of the mitochondria function-related genes were down-
regulated the most in the cells exposed for 1 h. After 24 h exposure, the number of
apoptotic cells and the relative levels of BAX to Bcl-2 increased. The expression
of SOD1 protein, but not of SOD2, also dose-dependently increased with an increase
in caspase-8 activity. Additionally, the MAPK pathway was significantly activated,
even though the expression of p-EGFR did not change significantly. Furthermore, the
number of apoptotic cells increased rapidly with time and with the inhibition of
vacuole formation. Taken together, we suggest that P25 and TNS may target different
organelles. In addition, TNS, but not P25, induced paraptosis accompanied by
apoptosis in MH-S cells, and the formation of the cytoplasmic vacuoles allowed
delay apoptosis following TNS exposure. (C) 2014 Elsevier Ireland Ltd. All rights
reserved.
AN - WOS:000341879900009
AU - Park, E. J.
AU - Lee, S. Y.
AU - Lee, G. H.
AU - Kim, D. W.
AU - Kim, Y.
AU - Cho, M. H.
AU - Kim, J. H.
DA - OCT 1
DO - 10.1016/j.toxlet.2014.07.027
IS - 1
PY - 2014
SN - 0378-4274
1879-3169
SP - 69-79
ST - Sheet-type titania, but not P25, induced paraptosis accompanying apoptosis in
murine alveolar macrophage cells
TI - Sheet-type titania, but not P25, induced paraptosis accompanying apoptosis in
murine alveolar macrophage cells
VL - 230
ID - 6362
ER -
TY - JOUR
AB - Acellular bacterial ghosts (BGs) are empty non-living bacterial cell
envelopes, commonly generated by controlled expression of the cloned lysis gene E
of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were
generated by chemically-induced lysis and the method is based on minimum inhibitory
concentration (MIC) of sodium hydroxide (NaOH), acetic acid, boric acid, citric
acid, maleic acid, hydrochloric acid, and sulfuric acid. The MIC values of the
respective chemicals were 3.125, 6.25, <50.0, 25.0, 6.25, 1.56, and 0.781 mg/mL.
Except for boric acid, the lysis efficiency reached more than 99.99% at 5 min after
treatment of all chemicals. Among those chemicals, NaOH-induced VPGs appeared
completely DNA-free, which was confirmed by quantitative real-time PCR. Besides,
lipopolysaccharides (LPS) extracted from the NaOH-induced VPGs showed no
distinctive band on SDS-PAGE gel after silver staining. On the other hand, LPS
extracted from wild-type bacterial cells, as well as the organic acids-induced VPGs
showed triple major bands and LPS extracted from the inorganic acids-induced VPGs
showed double bands. It suggests that some surface structures in LPS of the NaOH-
induced VPGs may be lost, weakened, or modified by the MIC of NaOH. Nevertheless,
Limulus amoebocyte lysate assay revealed that there is no significant difference in
endotoxic activity between the NaOH-induced VPGs and wild-type bacterial cells.
Macrophages exposed to the NaOH-induced VPGs at 0.5 x 10(6) CFU/mL showed cell
viability of 97.9%, however, the MIC of NaOH did not reduce the cytotoxic effect of
wild-type bacterial cells. Like Escherichia coli LPS, the NaOH-induced VPGs are an
excellent activator of pro-inflammatory cytokines (IL-1 beta and iNOS), anti-
inflammatory cytokine (IL-10), and dual activities (IL-6) in the stimulated
macrophage cells. On the other hand, the induction of TNF-beta mRNA was remarkable
in the macrophages exposed with wild-type cells. Scanning electron microscopy
showed the formation of trans-membrane lysis tunnel structures in the NaOH-induced
VPGs. SDS-PAGE and agarose gel electrophoresis also confirmed that cytoplasmic
proteins and genomic DNA released from the VPGs to culture medium through the lysis
tunnel structures. Taken together, all these data indicate that the NaOH-induced
VPGs show the potency of a safe, economical, and effective inactivated bacterial
vaccine candidate.
AN - WOS:000388809600135
AU - Park, H. J.
AU - Oh, S.
AU - Vinod, N.
AU - Ji, S. M.
AU - Noh, H. B.
AU - Koo, J. M.
AU - Lee, S. H.
AU - Kim, S. C.
AU - Lee, K. S.
AU - Choi, C. W.
C7 - 1904
DA - NOV
DO - 10.3390/ijms17111904
IS - 11
PY - 2016
SN - 1422-0067
ST - Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium
Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)
TI - Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium
Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)
VL - 17
ID - 6668
ER -
TY - JOUR
AB - Acellular bacterial ghosts (BGs) are empty non-living bacterial cell
envelopes, commonly generated by controlled expression of the cloned lysis gene E
of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were
generated by chemically-induced lysis and the method is based on minimum inhibitory
concentration (MIC) of sodium hydroxide (NaOH), acetic acid, boric acid, citric
acid, maleic acid, hydrochloric acid, and sulfuric acid. The MIC values of the
respective chemicals were 3.125, 6.25, <50.0, 25.0, 6.25, 1.56, and 0.781 mg/mL.
Except for boric acid, the lysis efficiency reached more than 99.99% at 5 min after
treatment of all chemicals. Among those chemicals, NaOH-induced VPGs appeared
completely DNA-free, which was confirmed by quantitative real-time PCR. Besides,
lipopolysaccharides (LPS) extracted from the NaOH-induced VPGs showed no
distinctive band on SDS-PAGE gel after silver staining. On the other hand, LPS
extracted from wild-type bacterial cells, as well as the organic acids-induced VPGs
showed triple major bands and LPS extracted from the inorganic acids-induced VPGs
showed double bands. It suggests that some surface structures in LPS of the NaOH-
induced VPGs may be lost, weakened, or modified by the MIC of NaOH. Nevertheless,
Limulus amoebocyte lysate assay revealed that there is no significant difference in
endotoxic activity between the NaOH-induced VPGs and wild-type bacterial cells.
Macrophages exposed to the NaOH-induced VPGs at 0.5 × 106 CFU/mL showed cell
viability of 97.9%, however, the MIC of NaOH did not reduce the cytotoxic effect of
wild-type bacterial cells. Like Escherichia coli LPS, the NaOH-induced VPGs are an
excellent activator of pro-inflammatory cytokines (IL-1β and iNOS), anti-
inflammatory cytokine (IL-10), and dual activities (IL-6) in the stimulated
macrophage cells. On the other hand, the induction of TNF-α mRNA was remarkable in
the macrophages exposed with wild-type cells. Scanning electron microscopy showed
the formation of trans-membrane lysis tunnel structures in the NaOH-induced VPGs.
SDS-PAGE and agarose gel electrophoresis also confirmed that cytoplasmic proteins
and genomic DNA released from the VPGs to culture medium through the lysis tunnel
structures. Taken together, all these data indicate that the NaOH-induced VPGs show
the potency of a safe, economical, and effective inactivated bacterial vaccine
candidate. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
AU - Park, H. J.
AU - Oh, S.
AU - Vinod, N.
AU - Ji, S.
AU - Noh, H. B.
AU - Koo, J. M.
AU - Lee, S. H.
AU - Kim, S. C.
AU - Lee, K. S.
AU - Choi, C. W.
C7 - 1904
DB - Scopus
DO - 10.3390/ijms17111904
IS - 11
KW - Bacterial ghosts (BGs)
Chemically-induced lysis
Cytokine
Cytotoxicity
Endotoxic activity
Lipopolysaccharides (LPS)
Macrophages
Minimum inhibition concentration (MIC)
Sodium hydroxide (NaOH)
Vibrio parahaemolyticus
Acetic Acid
Animals
Boric Acids
Cell Line
Cell Membrane
Cell Survival
Citric Acid
DNA, Bacterial
Gene Expression
Hydrochloric Acid
Interleukin-10
Interleukin-1beta
Interleukin-6
Limulus Test
Lipopolysaccharides
Maleates
Mice
Sodium Hydroxide
Sulfuric Acids
interleukin 1beta
lipopolysaccharide
nitric oxide synthase
acetic acid
bacterial DNA
boric acid
citric acid
hydrochloric acid
IL10 protein, mouse
IL1B protein, mouse
interleukin 10
interleukin 6
maleic acid
maleic acid derivative
Nos2 protein, mouse
sodium hydroxide
sulfuric acid
agar gel electrophoresis
Article
bacterial growth
controlled study
cytotoxicity
gene expression
immunogenicity
nonhuman
protein analysis
protein structure
animal
cell line
cell membrane
cell survival
chemistry
cytology
drug effects
genetics
immunology
Limulus lysate test
macrophage
metabolism
mouse
secretion (process)
M3 - Article
PY - 2016
ST - Characterization of chemically-induced bacterial ghosts (BGs) using sodium
hydroxide-induced Vibrio parahaemolyticus ghosts (VPGs)
TI - Characterization of chemically-induced bacterial ghosts (BGs) using sodium
hydroxide-induced Vibrio parahaemolyticus ghosts (VPGs)
84995679626&doi=10.3390%2fijms17111904&partnerID=40&md5=00ebbb26ba61c2127b82c2ae2e3
f4f0a
VL - 17
ID - 5499
ER -
TY - JOUR
AB - Commercialized dressing materials with or without silver have played a
passive role in early-phase wound healing, protecting the skin defects from
infections, absorbing exudate, and preventing dehydration. Chitosan (CTS)-based
sponges have been developed in pure or hybrid forms for accelerating wound healing,
but their wound-healing capabilities have not been extensively compared with widely
used commercial dressing materials, providing limited information in a practical
aspect. In this study, we have developed CTS-silica (CTS-Si) hybrid sponges with
water absorption, flexibility, and mechanical behavior similar to those of CTS
sponges. In vitro and in vivo tests were performed to compare the CTS-Si sponges
with three commercial dressing materials [gauze, polyurethane (PU), and silver-
containing hydrofiber (HF-Ag)] in addition to CTS sponges. Both in vitro and in
vivo tests showed that CTS-Si sponges promoted fibroblast proliferation, leading to
accelerated collagen synthesis, whereas the CTS sponges did not exhibit significant
differences in fibroblast proliferation and collagen synthesis from gauze, PU, and
HF-Ag sponges. In case of CTS-Si, the inflammatory cells were actively recruited to
the wound by the influence of the released silicon ions from CTS-Si sponges, which,
in return, led to an enhanced secretion of growth factors, particularly TGF- during
the early stage. The higher level of TGF- likely improved the proliferation of
fibroblasts, and as a result, collagen synthesis by fibroblasts became remarkably
productive, thereby increasing collagen density at the wound site. Therefore, the
CTS-Si hybrid sponges have considerable potential as a wound-dressing material for
accelerating wound healing. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res
Part B: Appl Biomater, 105B: 1828-1839, 2017.
AN - WOS:000409231400010
AU - Park, J. U.
AU - Jung, H. D.
AU - Song, E. H.
AU - Choi, T. H.
AU - Kim, H. E.
AU - Song, J.
AU - Kim, S.
DA - OCT
DO - 10.1002/jbm.b.33711
IS - 7
PY - 2017
SN - 1552-4973
1552-4981
SP - 1828-1839
ST - The accelerating effect of chitosan-silica hybrid dressing materials on the
early phase of wound healing
TI - The accelerating effect of chitosan-silica hybrid dressing materials on the
early phase of wound healing
VL - 105
ID - 6820
ER -
TY - JOUR
AB - The immune-response of macrophages is an important area of investigation
since it represents the major pathway by which early-stage defense barriers are
established in skin, lungs, and mucosal systems to counteract foreign objects. In
this study, we have examined the size-dependent inflammatory and toxicological
effects of nanostructured silver particles (nano-Ag) on macrophage immune cells. ©
2011 The Royal Society of Chemistry.
AU - Park, J.
AU - Lim, D. H.
AU - Lim, H. J.
AU - Kwon, T.
AU - Choi, J. S.
AU - Jeong, S.
AU - Choi, I. H.
AU - Cheon, J.
DB - Scopus
DO - 10.1039/c1cc10357a
IS - 15
KW - silver nanoparticle
article
cell viability
cytotoxicity
immune response
inflammation
macrophage
oxidative stress
particle size
M3 - Article
PY - 2011
SP - 4382-4384
ST - Size dependent macrophage responses and toxicological effects of Ag
nanoparticles
T2 - Chemical Communications
TI - Size dependent macrophage responses and toxicological effects of Ag
nanoparticles
79955023144&doi=10.1039%2fc1cc10357a&partnerID=40&md5=03a5674975f00bff936a4ba04472b
ea7
VL - 47
ID - 5659
ER -
TY - JOUR
AB - The biological response to four well-characterized amorphous silica
nanoparticles was investigated in RAW 264.7 macrophages in view of their potential
application as drug carriers to sites of inflammation. All silica nanoparticles-
induced cell membrane damage, reduced metabolic activity, generated ROS and
released various cytokines, but to different extents. Two silica nanoparticles of
34 nm (A and B) with different zetapotentials were more cytotoxic than (aggregated)
11 and 248 nm nanoparticles, while cytokines were mostly induced by the
(aggregated) 11 nm and only one of the 34 nm nanoparticles (34A). The results
indicate that specific silica nanoparticles may have counterproductive effects, for
example when used as carriers of anti-inflammatory drugs. The physicochemical
properties determining the response of nanoparticles vary for different responses,
implying that a screening approach for the safe development of nanoparticles needs
to consider the role of combinations of (dynamic) physicochemical properties and
needs to include multiple toxicity endpoints.
AN - WOS:000298056100053
AU - Park, Mvdz
AU - Lynch, I.
AU - Ramirez-Garcia, S.
AU - Dawson, K. A.
AU - de la Fonteyne, L.
AU - Gremmer, E.
AU - Slob, W.
AU - Briede, J. J.
AU - Elsaesser, A.
AU - Howard, C. V.
AU - van Loveren, H.
AU - de Jong, W. H.
DA - DEC
DO - 10.1007/s11051-011-0586-6
IS - 12
PY - 2011
SN - 1388-0764
1572-896X
SP - 6775-6787
ST - In vitro evaluation of cytotoxic and inflammatory properties of silica
nanoparticles of different sizes in murine RAW 264.7 macrophages
TI - In vitro evaluation of cytotoxic and inflammatory properties of silica
nanoparticles of different sizes in murine RAW 264.7 macrophages
VL - 13
ID - 6350
ER -
TY - JOUR
AB - Green nanotechnology is an ever-evolving field of research and development
due to an environmentally friendly approach. The present study highlights the green
synthesis using Pancratium parvum bulb extract for the biosynthesis of gold
nanoparticles and enlightens its thorough characterization with biomedical
applications. Here, the biogenic gold nanoparticles (AuNPs) were optimized for
maximum and rapid synthesis and characterized by employing several spectroscopic
and imaging techniques. Further, the synthesized particles were used to study anti-
oxidant, anti-inflammatory, chorioallantoic membrane assay (CAM), anti-
acetylcholinesterase enzyme assay, and binding kinetics study, comprising toxicity
study on Vero cell lines. The findings concluded that statistically optimized
parameters exhibited optimum synthesis of AuNPs with promising anti-oxidant, anti-
inflammatory properties, and potential acetylcholinesterase inhibition as a remedy
for Alzheimer's disease. In addition, surface plasmon resonance (SPR) studies
revealed good affinity kinetics of immobilized acetylcholinesterase enzyme on
sensor chip with KD value 1.449 x 10(-6) M. Concurrently, the effect of AuNPs was
evaluated on Vero cell lines, which exhibited the viability of cells at higher
concentration, and CAM assay did not demonstrate angiogenicity. Thus, this said
approach is rapid with potential medical applications.
AN - WOS:000639072800001
AU - Patil, D. N.
AU - Patil, P. J.
AU - Rane, M. R.
AU - Yadav, S. R.
AU - Bapat, V. A.
AU - Vyavahare, G. D.
AU - Jadhav, J. P.
C6 - APR 2021
DA - SEP
DO - 10.1007/s40089-021-00335-z
IS - 3
PY - 2021
SN - 2008-9295
2228-5326
SP - 215-232
ST - Response surface methodology-based optimization of Pancratium parvum Dalzell-
mediated synthesis of gold nanoparticles with potential biomedical applications
T2 - INTERNATIONAL NANO LETTERS
TI - Response surface methodology-based optimization of Pancratium parvum Dalzell-
mediated synthesis of gold nanoparticles with potential biomedical applications
VL - 11
ID - 6504
ER -
TY - JOUR
AB - Prolonged inflammation and infection are the major factors that promote
chronicity of the wound. Despite the substantial advancements in therapeutic
modalities, the treatment of chronic wounds still represents a major clinical
challenge. Conventional remedies are associated with several complications like
drug resistance, sys-temic toxicity, and serious side effects that need to be
addressed. This necessitates the development of safe and alternative pro-healing
agents for efficient wound repair. In this scenario, the application of
phytoconstituents has garnered much attention due to their safety profile and cost-
effectiveness. They enhance the wound repair process by modulating various
signaling pathways like TGF-beta, NF kappa B, Nrf2, MAPK, etc, and promote cell
pro-liferation, migration, differentiation, angiogenesis, and inhibit inflammation.
The topical route of drug admin-istration is the most preferable method of drug
delivery for the effective management of chronic wounds. Currently, much emphasis
has been given to the formulation of different phytoconstituents-based topical
delivery systems like hydrogels, films, foam, sponges, fibers, nanoformulations,
etc. These topical formulations improve the pharmacokinetic and physicochemical
features of phytoconstituents. The main emphasis of the present re-view is to
discuss the therapeutic potential, pharmacological importance, and current clinical
status of various phytoconstituents-based topical formulations as well as their
associated molecular mechanisms in chronic wound management.
AN - WOS:001003645900001
AU - Pattnaik, S.
AU - Mohanty, S.
AU - Sahoo, S. K.
AU - Mohanty, C.
C6 - MAY 2023
C7 - 104546
DA - JUN
DO - 10.1016/j.jddst.2023.104546
PY - 2023
SN - 1773-2247
2588-8943
ST - A mechanistic perspective on the role of phytoconstituents-based
pharmacotherapeutics and their topical formulations in chronic wound management
T2 - JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
TI - A mechanistic perspective on the role of phytoconstituents-based
pharmacotherapeutics and their topical formulations in chronic wound management
VL - 84
ID - 6566
ER -
TY - JOUR
AB - With advancements in nanotechnology, silver has been engineered into a
nanometre size and has attracted great research interest for use in the treatment
of wounds. Silver nanoparticles (AgNPs) have emerged as a potential alternative to
conventional antibiotics because of their potential antimicrobial property.
However, AgNPs also induce cytotoxicity, generate reactive oxygen species (ROS),
and cause mitochondrial damage to human cells. Pyridoxine possesses antioxidant and
cell proliferation activity. Therefore, in the present investigation, a nanosilver-
pyridoxine complex (AgPyNP) was synthesized, and its cytotoxicity and immune
response was compared with AgNPs in macrophage RAW264.7 cells. Results revealed
that AgPyNPs showed less cytotoxicity compared with AgNPs by producing a smaller
amount of ROS in RAW264.7 cells. Surprisingly, however, AgPyNPs caused macrophage
RAW264.7 cells to secrete a larger amount of interleukin-8 (IL-8) and generate a
more active inflammatory response compared to AgNPs. It activated TNF-α, NF-κB p65,
and NF-κB p50 to generate a more vigorous immune protection that produces a greater
amount of IL-8 compared to AgNPs. Overall findings indicate that AgPyNPs exhibited
less cytotoxicity and evoked a greater immune response in macrophage RAW264.7
cells. Thus, it can be used as a better wound-healing agent than AgNPs.; [Figure
not available: see fulltext.] © 2015, Paul et al.; licensee Springer.
AU - Paul, A.
AU - Ju, H.
AU - Rangasamy, S.
AU - Shim, Y.
AU - Song, J. M.
C7 - 140
DB - Scopus
DO - 10.1186/s11671-015-0848-9
IS - 1
KW - IL-8
Macrophage
ROS
Silver pyridoxine nanoparticles
Cell proliferation
Cytology
Cytotoxicity
Immune system
Macrophages
Metal nanoparticles
Macrophage RAW264.7
Mitochondrial damage
Proliferation activity
Silver compounds
M3 - Article
PY - 2015
ST - Nanosized silver (II) pyridoxine complex to cause greater inflammatory
response and less cytotoxicity to RAW264.7 macrophage cells
TI - Nanosized silver (II) pyridoxine complex to cause greater inflammatory
response and less cytotoxicity to RAW264.7 macrophage cells
84925797437&doi=10.1186%2fs11671-015-0848-
9&partnerID=40&md5=d9bc44b370ab6694a70402da0f264f6f
VL - 10
ID - 5606
ER -
TY - JOUR
AB - Aim: Zinc oxide nanoparticles can be multidimensionally used for treatment,
targeting and diagnosis of lung, breast, skin and many other cancer or tumors.
Coriander oleoresin is derived from dried seeds of Coriandrum sativum. Common uses
of coriander include its application as a diuretic, an antidepressant and a
memoryenhancing compound. Also, it has been shown to exhibit great antioxidative
capacity due to its polyphenolic compounds and further antidiabetic and
antimutagenic effects. The aim of the present study was to evaluate the
antibacterial activity and cytotoxic activity of coriander oleoresin mediated ZnO
nanoparticles. Materials and methods: In the present investigation, coriander
oleoresin mediated synthesis of ZnO nanoparticles was carried out initially and
then confirmed by UV-Visible spectroscopy. The antibacterial activity of the
synthesis ZnO nanoparticles was carried out using agar well diffusion method.
Different concentrations of zinc nanoparticles were tested against Streptococcus
mutans, lactobacillus and Candida albicans. Results: The zinc nanoparticles
prepared from coriander oleoresin showed good anti-inflammatory activity. The zinc
nanoparticles were analysed using UV- Visible spectroscopy and were found to be at
an absorbance range of 300 to 700nm. The peak was found to be a maximum of 350 nm.
The synthesised nanoparticles showed maximum anti-bacterial activity with 9 mm at
150 μL concentration. The study did not exhibit any cytotoxic activity. Conclusion:
In the present study, it was observed that coriander oleoresin mediated zinc
nanoparticles had antibacterial property and also did not exhibit any cytotoxic
activity. Green synthesis of Zinc nanoparticles using coriander oleoresin was of
low cost and was convenient to carry out. Hence, coriander oleoresin mediated zinc
nanoparticles may be used for the control of oral pathogens in large scales. ©
2020, Advanced Scientific Research. All rights reserved.
AU - Paul, R.
AU - Roy, A.
AU - Thangavelu, L.
DB - Scopus
DO - 10.31838/ijpr/2020.SP1.411
KW - Green synthesis
Nanoparticles
Oleoresin
Oral pathogens
ZnO nanoparticles
aciclovir
amoxicillin
antidepressant agent
antidiabetic agent
antioxidant
diuretic agent
nanoparticle
plant extract
polyphenol derivative
silver nanoparticle
zinc
zinc nanoparticle
agar diffusion
agar dilution
antifungal activity
antimutagenic activity
Article
Bacillus subtilis
bacteriophage
breast
Candida albicans
cell viability
controlled study
coriander
cytotoxicity
drug synthesis
Escherichia coli
infectious agent
Lactobacillus
LC50
lung
malignant neoplasm
MTT assay
nonhuman
phytochemistry
shrimp
skin
Streptococcus mutans
zone of inhibition
M3 - Article
PY - 2020
SP - 3057-3062
ST - Cytotoxic effect and antibacterial activity of coriander oleoresin mediated
zinc oxide nanoparticles
T2 - International Journal of Pharmaceutical Research
TI - Cytotoxic effect and antibacterial activity of coriander oleoresin mediated
zinc oxide nanoparticles
85104899497&doi=10.31838%2fijpr
%2f2020.SP1.411&partnerID=40&md5=7a24a446c92485d13cc1289ac3538367
VL - 12
ID - 5269
ER -
TY - JOUR
AB - Although a variety of nanoparticles (NPs) functionalized with amphotericin B,
an antifungal agent widely used in the clinic, have been studied in the last years
their cytotoxicity profile remains elusive. Here we show that human endothelial
cells take up high amounts of silica nanoparticles (SNPs) conjugated with
amphotericin B (AmB) (SNP-AmB) (65.4 +/- 12.4 pg of Si per cell) through
macropinocytosis while human fibroblasts internalize relatively low amounts (2.3
+/- 0.4 pg of Si per cell) because of their low capacity for macropinocytosis. We
further show that concentrations of SNP-AmB and SNP up to 400 mu g/mL do not
substantially affect fibroblasts. In contrast, endothelial cells are sensitive to
low concentrations of NPs (above 10 mu g/mL), in particular to SNP-AmB. This is
because of their capacity to internalize high concentration of NPs and high
sensitivity of their membrane to the effects of AmB. Low-moderate concentrations of
SNP-AmB (up to 100 mu g/mL) induce the production of reactive oxygen species (ROS),
LDH release, high expression of pro-inflammatory cytokines and chemokines (IL-8,
IL-6, G-CSF, CCL4, IL-1 beta and CSF2) and high expression of heat shock proteins
(HSPs) at gene and protein levels. High concentrations of SNP-AmB (above 100 mu
g/mL) disturb membrane integrity and kill rapidly human cells (60% after 5 h). This
effect is higher in SNP-AmB than in SNP. (C) 2013 Elsevier Ltd. All rights
reserved.
AN - WOS:000319630000038
AU - Paulo, C. S. O.
AU - Lino, M. M.
AU - Matos, A. A.
AU - Ferreira, L. S.
DA - JUL
IS - 21
PY - 2013
SN - 0142-9612
1878-5905
SP - 5281-5293
ST - Differential internalization of amphotericin B - Conjugated nanoparticles in
human cells and the expression of heat shock protein 70
T2 - BIOMATERIALS
TI - Differential internalization of amphotericin B - Conjugated nanoparticles in
human cells and the expression of heat shock protein 70
VL - 34
ID - 6449
ER -
TY - JOUR
AB - Alongside physiochemical properties (PCP), it has been suggested that the
protein corona of nanoparticles (NPs) plays a crucial role in the response of
immune cells to NPs. However, due to the great variety of NPs, target cells, and
exposure protocols, there is still no clear relationship between PCP, protein
corona composition, and the immunotoxicity of NPs. In this study, we correlated PCP
and the protein corona composition of NPs to the THP-1 macrophage response,
focusing on selected toxicological endpoints: cell viability, reactive oxygen
species (ROS), and cytokine secretion. We analyzed seven commonly used engineered
NPs (SiO2, silver, and TiO2) and magnetic NPs. We show that with the exception of
silver NPs, all of the tested TiO2 types and SiO2 exhibited moderate toxicities and
a transient inflammatory response that was observed as an increase in ROS, IL-8,
and/or IL-1 beta cytokine secretion. We observed a strong correlation between the
size of the NPs in media and IL-1 beta secretion. The induction of IL-1 beta
secretion was completely blunted in NLR family pyrin domain containing 3 (NLRP3)
knockout THP-1 cells, indicating activation of the inflammasome. The correlations
analysis also implicated the association of specific NP corona proteins with the
induction of cytokine secretion. This study provides new insights toward a better
understanding of the relationships between PCP, protein corona, and the
inflammatory response of macrophages for different engineered NPs, to which we are
exposed on a daily basis.
AN - WOS:000809927300001
AU - Pavlin, M.
AU - Lojk, J.
AU - Strojan, K.
AU - Hafner-Bratkovic, I.
AU - Jerala, R.
AU - Leonardi, A.
AU - Krizaj, I.
AU - Drnovsek, N.
AU - Novak, S.
AU - Veranic, P.
AU - Bregar, V. B.
C7 - 6197
DA - JUN
DO - 10.3390/ijms23116197
IS - 11
PY - 2022
SN - 1422-0067
ST - The Relevance of Physico-Chemical Properties and Protein Corona for
Evaluation of Nanoparticles Immunotoxicity-In Vitro Correlation Analysis on THP-1
Macrophages
TI - The Relevance of Physico-Chemical Properties and Protein Corona for
Evaluation of Nanoparticles Immunotoxicity-In Vitro Correlation Analysis on THP-1
Macrophages
VL - 23
ID - 6587
ER -
TY - JOUR
AB - Alongside physiochemical properties (PCP), it has been suggested that the
protein corona of nanoparticles (NPs) plays a crucial role in the response of
immune cells to NPs. However, due to the great variety of NPs, target cells, and
exposure protocols, there is still no clear relationship between PCP, protein
corona composition, and the immunotoxicity of NPs. In this study, we correlated PCP
and the protein corona composition of NPs to the THP-1 macrophage response,
focusing on selected toxicological endpoints: cell viability, reactive oxygen
species (ROS), and cytokine secretion. We analyzed seven commonly used engineered
NPs (SiO2, silver, and TiO2) and magnetic NPs. We show that with the exception of
silver NPs, all of the tested TiO2 types and SiO2 exhibited moderate toxicities and
a transient inflammatory response that was observed as an increase in ROS, IL-8,
and/or IL-1β cytokine secretion. We observed a strong correlation between the size
of the NPs in media and IL-1β secretion. The induction of IL-1β secretion was
completely blunted in NLR family pyrin domain containing 3 (NLRP3) knockout THP-1
cells, indicating activation of the inflammasome. The correlations analysis also
implicated the association of specific NP corona proteins with the induction of
cytokine secretion. This study provides new insights toward a better understanding
of the relationships between PCP, protein corona, and the inflammatory response of
macrophages for different engineered NPs, to which we are exposed on a daily basis.
AU - Pavlin, M.
AU - Lojk, J.
AU - Strojan, K.
AU - Hafner-Bratkovič, I.
AU - Jerala, R.
AU - Leonardi, A.
AU - Križaj, I.
AU - Drnovšek, N.
AU - Novak, S.
AU - Veranič, P.
AU - Bregar, V. B.
C7 - 6197
DB - Scopus
DO - 10.3390/ijms23116197
IS - 11
KW - correlation
cytokines
immune response
inflammasome
macrophages
nanoparticles
nanotoxicology
physico-chemical properties
protein corona
TiO2
cryopyrin
interleukin 1beta
interleukin 8
nanoparticle
silica nanoparticle
silver
titanium dioxide
Article
cell viability
controlled study
cytokine release
human
human cell
immunotoxicity
in vitro study
inflammation
macrophage
protein analysis
THP-1 cell line
M3 - Article
PY - 2022
ST - The Relevance of Physico-Chemical Properties and Protein Corona for
Evaluation of Nanoparticles Immunotoxicity—In Vitro Correlation Analysis on THP-1
Macrophages
TI - The Relevance of Physico-Chemical Properties and Protein Corona for
Evaluation of Nanoparticles Immunotoxicity—In Vitro Correlation Analysis on THP-1
Macrophages
85131754392&doi=10.3390%2fijms23116197&partnerID=40&md5=8952656997f33eb069cbf7572ef
fe2ce
VL - 23
ID - 5111
ER -
TY - JOUR
AB - The objective of this study was to prepare a new biodegradable Mg-based
biomaterial, which provides good mechanical integrity in combination with anti-
inflammatory function during the degradation process. The silver element was used,
because it improved the mechanical properties as an effective grain refiner and it
is also treated as a potential anti-inflammatory core. The new degradable Mg-Zn-Ag
biomaterial was prepared by zone solidification technology and extrusion. The
mechanical properties were mostly enhanced by fine grain strengthening. In
addition, the alloys exhibited good cytocompatibility. The anti-inflammatory
function of degradation products was identified by both interleukin-1α and nitric
oxide modes. The anti-inflammatory impact was significantly associated with the
concentration of silver ion. It was demonstrated that Mg-Zn-Ag system was a
potential metallic stent with anti-inflammatory function, which can reduce the
long-term dependence of anti-inflammatory drug after coronary stent implantation.
Copyright © 2012 Wiley Periodicals, Inc.
AU - Peng, Q.
AU - Li, K.
AU - Han, Z.
AU - Wang, E.
AU - Xu, Z.
AU - Liu, R.
AU - Tian, Y.
DB - Scopus
DO - 10.1002/jbm.a.34494
IS - 7
degradation properties
mechanical properties
metallic implant
Alloys
Body Fluids
Cell Survival
Drug Implants
Electrochemistry
Hardness
HeLa Cells
Humans
Indicators and Reagents
Interleukin-1alpha
Macrophages
Magnesium Compounds
Mechanical Processes
Nitric Oxide
Particle Size
Silver Compounds
Solutions
Zinc Compounds
Biological materials
Degradation
Metal ions
Nitric oxide
Silver
Stents
Zinc
alloy
biomaterial
interleukin 1alpha
magnesium
nitric oxide
silver
zinc
Anti-inflammatory function
Degradation products
Fine grain strengthening
Long-term dependence
Mechanical integrity
Metallic implants
article
biocompatibility
biomechanics
chemical structure
compression
controlled study
coronary stent
cytokine release
degradation
female
hardness
human
human cell
in vitro study
metal stent
tensile strength
Young modulus
Silver alloys
M3 - Article
PY - 2013
SP - 1898-1906
ST - Degradable magnesium-based implant materials with anti-inflammatory activity
TI - Degradable magnesium-based implant materials with anti-inflammatory activity
84878293202&doi=10.1002%2fjbm.a.34494&partnerID=40&md5=679c2d35e3c411a1a823bd5afde8
868a
VL - 101 A
ID - 5690
ER -
TY - JOUR
AB - The skin represents our first defense against physical, mechanical, or
biological damage. However, its regeneration potential depends on the degree of the
wound and the systemic state. For large acute or chronic wounds, specialized
treatments are required to stimulate regeneration and avoid scarring. The use of
nanotechnology represents a promising tool to promote wound healing, not only by
developing antibacterial nanomaterials to prevent infection, but also by developing
nanomaterials capable of enhancing cell proliferation, extracellular matrix
deposition, and angiogenesis, as well as regulating inflammation. Nanotechnology
offers a new and wide field of application for materials, due to the unique
properties of nanomaterials that normally differ from, or are enhanced compared to,
bulk materials. Nanomaterial properties changed mainly due to its larger contact
area, which normally results in better optical, catalytic, and biodistribution
properties. Due to their physical–chemical characteristics, nanoparticles can be
tailored in different ways to be delivered and act at the site of injury, improving
wound healing. Nanoparticles can be incorporated into different substrates to
generate antibacterial coverages, they can be functionalized and tailored to be
used as vectors for delivering signaling molecules (growth factors), or their
properties such as size, crystallinity, surface chemistry, and shape can be
modified to elicit different biological effects. The present work is aimed at
resuming the advances of the last decade in nanoparticles used to improve wound
healing based on their antimicrobial activity, cell proliferation enhancement,
angiogenesis promotion, regulation of extracellular matrix deposition, and anti-
inflammatory modulation. Graphical abstract: [Figure not available: see fulltext.].
© 2023, The Author(s), under exclusive licence to Springer Nature B.V.
AU - Pérez-Díaz, M. A.
AU - Prado-Prone, G.
AU - Díaz-Ballesteros, A.
AU - González-Torres, M.
AU - Silva-Bermudez, P.
AU - Sánchez-Sánchez, R.
C7 - 27
DB - Scopus
DO - 10.1007/s11051-023-05675-9
IS - 2
KW - Antibacterial
Cell proliferation
Inflammation
Nanobiomedicine
Nanoparticles
Wound healing
Crystallinity
Deposition
Pathology
Surface chemistry
Tissue regeneration
alginic acid
carbomer
chitosan
cycline
cytokine
dimethyloxalylglycine
gelatin
hexagonal boron nitride
hyaluronic acid
hydrogel
interleukin 1
metal nanoparticle
nanocomposite
nanofiber
nanomaterial
nanoparticle
Pluronic 127
polycaprolactone
polylactic acid nanoparticles
polymyxin
pyridoxine
sericin
serpin A1
silica nanoparticle
silver nanoparticle
sonic hedgehog protein
titanium oxide nanoparticle
tumor necrosis factor alpha receptor
unclassified drug
vasculotropin receptor
Angiogenesis
Antibacterials
Chronic wounds
Extracellular matrix deposition
Mechanical
Property
Wound healing process
3T3 cell line
angiogenesis
angiogenesis improvement
antiinflammatory modulation
apoptosis
bacterial infection control
biodistribution
blood vessel density
cell proliferation enhancement
cell infiltration
cell proliferation
cell viability
chorioallantoic membrane assay
colony forming unit
conductive property
cytotoxicity
Enterobacter cloacae
epithelization
Escherichia coli
extracellular matrix deposition
fibroblast
fibroblast 3T3 cell line
granulation tissue
HaCat cell line
human
immune response
keratinization
keratinocyte
mammal cell
nanobiomedicine
nanotechnology
neonatal human dermal fibroblast
NIH 3T3 cell line
nonhuman
Review
tissue integrity
tubular structure
wound closure
wound healing
zone of inhibition
M3 - Review
PY - 2023
ST - Nanoparticle and nanomaterial involvement during the wound healing process:
an update in the field
TI - Nanoparticle and nanomaterial involvement during the wound healing process:
an update in the field
85146876433&doi=10.1007%2fs11051-023-05675-
9&partnerID=40&md5=c21a083cfb64c54db2fbfe3749df4b69
VL - 25
ID - 4977
ER -
TY - JOUR
AB - The purpose of this study was to compare the intraosseous biocompatibility of
Dyract, a new hydrophilic glass-ionomer cement, to that of Super EBA. Twenty-four
New Zealand rabbits were anesthetized, one leg was shaved, the femur exposed, and
two holes were drilled through the cortical plate, The materials were loaded into
silicone carriers and inserted into the femur. Half of the rabbits were killed 4
weeks after implantation and the other half at 12 weeks and the femurs were
prepared using standard histological procedures, The tissue reactions were graded
from none to severe, At 4 weeks both materials showed slight to moderate reactions,
characterized by the presence of fibrous tissue interposition and inflammatory
cells. At 12 weeks, bone healing had occurred, despite the persistence of some
fibrous tissue interposition, and the reactions were classified as slight. At both
observation periods, statistical analysis failed to show any difference between the
two materials indicating that Dyract and Super EBA had similar intraosseous
biocompatibility.
AN - WOS:A1997WW73700005
AU - Pertot, W. J.
AU - Stephan, G.
AU - Tardieu, C.
AU - Proust, J. P.
DA - MAY
DO - 10.1016/S0099-2399(97)80413-X
IS - 5
PY - 1997
SN - 0099-2399
1878-3554
SP - 315-319
ST - Comparison of the intraosseous biocompatibility of dyract and super EBA
TI - Comparison of the intraosseous biocompatibility of dyract and super EBA
VL - 23
ID - 6343
ER -
TY - JOUR
AB - The surface topographies of nanoporous anodic aluminum oxide (AAO) and
titanium dioxide (TiO2) membranes have been shown to modulate cell response in
orthopedic and skin wound repair applications. In this study, we: (1) demonstrate
an improved atomic layer deposition (ALD) method for coating the porous structures
of 20, 100, and 200 nm pore diameter AAO with nanometer-thick layers of TiO2 and
(2) evaluate the effects of uncoated AAO and TiO2-coated AAO on cellular responses.
The TiO2 coatings were deposited on the AAO membranes without compromising the
openings of the nanoscale pores. The 20 nm TiO2-coated membranes showed the highest
amount of initial protein adsorption via the micro bicinchoninic acid (micro-BOA)
assay; all of the TiO2-coated membranes showed slightly higher protein adsorption
than the uncoated control materials. Cell viability, proliferation, and
inflammatory responses on the TiO2-coated AAO membranes showed no adverse outcomes.
For all of the tested surfaces, normal increases in proliferation (DNA content) of
L929 fibroblasts were observed over from 4 hours to 72 hours. No increases in TNF-
alpha production were seen in RAW 264.7 macrophages grown on TiO2-coated AAO
membranes compared to uncoated AAO membranes and tissue culture polystyrene (TOPS)
surfaces. Both uncoated AAO membranes and TiO2-coated AAO membranes showed no
significant effects on cell growth and inflammatory responses. The results suggest
that TiO2-coated AAO may serve as a reasonable prototype material for the
development of nanostructured wound repair devices and orthopedic implants.
AN - WOS:000361931700016
AU - Petrochenko, P. E.
AU - Kumar, G.
AU - Fu, W. J.
AU - Zhang, Q.
AU - Zheng, J. W.
AU - Liang, C. D.
AU - Goering, P. L.
AU - Narayan, R. J.
DA - DEC
DO - 10.1166/jbn.2015.2169
IS - 12
PY - 2015
SN - 1550-7033
1550-7041
SP - 2275-2285
ST - Nanoporous Aluminum Oxide Membranes Coated with Atomic Layer Deposition-Grown
Titanium Dioxide for Biomedical Applications: An In Vitro Evaluation
TI - Nanoporous Aluminum Oxide Membranes Coated with Atomic Layer Deposition-Grown
Titanium Dioxide for Biomedical Applications: An In Vitro Evaluation
VL - 11
ID - 6601
ER -
TY - JOUR
AB - The chronic toxicity test has been conducted for twenty-eight days to
characterize the hepatic expression levels of eight stress-related genes after
exposing Medaka to two doses of silver nitrate or a silver nanoparticle (Ag-NP)
using real time RT-PCR analysis. This extends our previously published work to
include three additional biomarkers and three later time points. In comparing with
the control, the significant induction of MT and GST genes in livers of the fish
exposed to 1μg/l Ag-NPs was observed at various time points during the test period.
The Orla C3-1 (Medaka) gene was slightly induced only with 1μg/l Ag-NPs at 7-day
exposure while the suppression of p53 and HSP70 was recorded in all exposures at
the end of the test. The gene encoding transferrin was repressed at day 21 by both
silver types and at every exposure dosage. These results revealed that the Ag-NPs
increase metal detoxification, oxidative and inflammatory stress, and finally
stimulate immune responses in Medaka. The conspicuous induction of choriogenin L
and vitellogenin 1 in male fish exposed to Ag-NPs, especially at 7- and 21-day,
compared with the exposures of AgNO 3 or control was the first attempt to examine
estrogenic effects of Ag-NPs. © 2011 Elsevier Inc.
AU - Pham, C. H.
AU - Yi, J.
AU - Gu, M. B.
DB - Scopus
DO - 10.1016/j.ecoenv.2011.11.034
KW - Biomarker
Chronic toxicity
Medaka
Nanotoxicity
Real-time PCR
Silver nanoparticle
Animals
Biological Markers
Estrogens
Gene Expression
Liver
Male
Metal Nanoparticles
Oryzias
Silver
Vitellogenins
Oryzias latipes
Oryziinae
protein p53
silver nanoparticle
silver nitrate
transferrin
unclassified drug
vitellogenin
vitellogenin 1
biomarker
estrogenic compound
fish
gene
nitrate
silver
toxicity test
animal cell
article
biomarker gene
chronic toxicity
controlled study
cytotoxicity test
detoxification
gene expression
gene location
gene repression
GST gene
immune response
immunostimulation
inflammation
liver
male
marker gene
mt gene
nonhuman
nucleotide sequence
Orla C3 1 gene
oxidative stress
M3 - Article
PY - 2012
SP - 239-245
ST - Biomarker gene response in male Medaka (Oryzias latipes) chronically exposed
to silver nanoparticle
TI - Biomarker gene response in male Medaka (Oryzias latipes) chronically exposed
to silver nanoparticle
84857444151&doi=10.1016%2fj.ecoenv.2011.11.034&partnerID=40&md5=cd099d9e933dd775848
6103675a0b625
VL - 78
ID - 5739
ER -
TY - JOUR
AB - Plant secondary metabolites such as flavonoids demonstrate high degrees of
antioxidant, anti-inflammatory, and anticancer activities. Among flavonoids,
quercetin plays an important role in inflammation by downregulating the level of
various cytokines. Thereby, in this work, onion (Allium cepa) peel was successfully
utilized for the synthesis of gold nano-bioconjugates acting as a natural
therapeutic drug. In this process, crude onion peel extract was first divided into
different fractionates, namely, ethyl acetate, butanol, methanol, and water, and
they were subjected to various preliminary studies of antioxidant activities. The
ethyl acetate fractionate shows high antioxidant activities in all the assays. The
bioactive components were identified and found to contain a high amount of
quercetin as confirmed by liquid chromatography with tandem mass spectrometry and
high-performance liquid chromatogrpahy. Three gold nano-bioconjugates were prepared
with different concentrations of the ethyl acetate fractionate. Various biochemical
anti-inflammatory assays were carried out and compared with the active ethyl
acetate fraction of the onion peel drug (OPD). The cytotoxicity of the nano-
bioconjugate system and the OPD was checked in the myoblast L6 cell line from
skeletal muscle tissues to evaluate the toxicity. All the three nano-bioconjugates
A, B, and E demonstrated high percentages of cell viability, viz., 73.07, 72.3, and
69.15%, respectively, at their highest concentration of 200 mu g/mL. The OPD also
showed 88.56% cell viability with no toxic effects in the myoblast L6 cell line
from skeletal muscle tissues. The reactive oxygen species reduction of
nanobioconjugate B showed a marked reduction of 76.77% at a maximum concentration
of 200 mu g/mL, whereas the OPD showed 68.17%. Hence, through this work, a cheap
source of nano-bioconjugates is developed, which can act as a potent antioxidant
and anti-inflammatory agent and are more active in comparison to the OPD alone.
AN - WOS:000677481800006
AU - Phukan, K.
AU - Devi, R.
AU - Chowdhury, D.
C6 - JUL 2021
DA - JUL 20
DO - 10.1021/acsomega.1c00861
IS - 28
PY - 2021
SN - 2470-1343
SP - 17811-17823
ST - Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and
Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies
T2 - ACS OMEGA
TI - Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and
Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies
VL - 6
ID - 6156
ER -
TY - JOUR
AB - Two gold(I) complexes featuring 1,2,4-triazole derived carbenes ([AuCl(Cy-
tazy)] and [AuCl(Bn-tazy)]) have been successfully synthesized via a silver-carbene
transfer pathway. Subsequent metal oxidations of using PhICl2 resulted in gold(III)
complexes [AuCl3(Cy-tazy)] and [AuCl3(Bn-tazy)], respectively. All the complexes
have been characterized by elemental analysis, multinuclear NMR spectroscopy, (ESI)
mass spectrometry, single crystal X-ray diffraction and DFT calculations. In
addition, all the four gold complexes exhibit high antiproliferative activities
against KB, MCF-7 and HT-29 cancerous cell lines. The most active compound,
[AuCl(Bn-tazy)], is also potent to Hela and leukemia (HL-60) cells but shows lower
degree of cytotoxicity toward non-cancerous human embryonic kidney cells (HEK-293).
Our preliminary studies also reveal that the two gold(III) complexes are capable of
stabilizing red blood cell membrane and suppressing the NO production in RAW264.7
macrophages induced by lipopolysaccharides, suggesting their potential anti-
inflammatory activities. Interestingly, there have hardly been any reports on anti-
inflammatory activities of gold(III) complexes of N-heterocyclic carbenes. © 2023
Elsevier B.V.
AU - Phung, H. T. T.
AU - Vu, H. M.
AU - Ly, M. Q. H.
AU - Nguyen, H. H.
AU - Nguyen, T. H.
AU - Luong, H. T. T.
AU - Nguyen, V. H.
C7 - 110898
DB - Scopus
DO - 10.1016/j.inoche.2023.110898
M3 - Article
PY - 2023
ST - Gold(I) and gold(III) complexes of 1,2,4-triazole-derived N-heterocyclic
carbenes: Synthesis, characterization, in-vitro anticancer and anti-inflammatory
studies
T2 - Inorganic Chemistry Communications
TI - Gold(I) and gold(III) complexes of 1,2,4-triazole-derived N-heterocyclic
carbenes: Synthesis, characterization, in-vitro anticancer and anti-inflammatory
studies
85161650795&doi=10.1016%2fj.inoche.2023.110898&partnerID=40&md5=99ad38014423b9e0605
0b82019272067
VL - 154
ID - 5052
ER -
TY - JOUR
AB - Silver tungstate (α-Ag2WO4), silver molybdate (β-Ag2MoO4), and silver
vanadate (α-AgVO3) microcrystals have shown interesting antimicrobial properties.
However, their biocompatibility is not yet fully understood. Cytotoxicity and the
inflammatory response of silver-containing microcrystals were analyzed in THP-1 and
THP-1 differentiated as macrophage-like cells, with the alamarBlue™ assay, flow
cytometry, confocal microscopy, and ELISA. The present investigation also evaluated
redox signaling and the production of cytokines (TNFα, IL-1β, IL-6, and IL-8) and
matrix metalloproteinases (MMP-8 and -9). The results showed that α-AgVO3
(3.9 μg/mL) did not affect cell viability (p > 0.05). α-Ag2WO4 (7.81 μg/mL), β-
Ag2MoO4 (15.62 μg/mL), and α-AgVO3 (15.62 μg/mL) slightly decreased cell viability
(p ≤ 0.003). All silver-containing microcrystals induced the production of O2− and
this effect was mitigated by Reactive Oxygen Species (ROS) scavenger and N-
acetylcysteine (NAC). TNFα, IL-6 and IL-1β were not detected in THP-1 cells, while
their production was either lower (p ≤ 0.0321) or similar to the control group (p ≥
0.1048) for macrophage-like cells. The production of IL-8 by both cellular
phenotypes was similar to the control group (p ≥ 0.3570). The release of MMP-8 was
not detected in any condition in THP-1 cells. Although MMP-9 was released by THP-1
cells exposed to α-AgVO3 (3.9 μg/mL), no significant difference was found with
control (p = 0.7). Regarding macrophage-like cells, the release of MMP-8 and -9
decreased in the presence of all microcrystals (p ≤ 0.010). Overall, the present
work shows a promising biocompatibility profile of, α-Ag2WO4, β-Ag2MoO4, and α-
AgVO3 microcrystals. Copyright © 2023 Pimentel, De Annunzio, Assis, Barbugli, Longo
and Vergani.
AU - Pimentel, B. N. A. D. S.
AU - De Annunzio, S. R.
AU - Assis, M.
AU - Barbugli, P. A.
AU - Longo, E.
AU - Vergani, C. E.
C7 - 1215438
DB - Scopus
DO - 10.3389/fbioe.2023.1215438
KW - cytokines
macrophages
matrix metalloproteinases, reactive oxygen species
monocytes
silver-based metal oxides
Biocompatibility
Cell signaling
Cytology
Microcrystals
Tungsten compounds
Cytokines
Macrophage-like cells
matrix
Matrix metalloproteinase, reactive oxygen species
Metal-oxide
Metalloproteinases
Monocyte
Silver-based metal oxide
THP-1 cells
Macrophages
M3 - Article
PY - 2023
ST - Biocompatibility and inflammatory response of silver tungstate, silver
molybdate, and silver vanadate microcrystals
T2 - Frontiers in Bioengineering and Biotechnology
TI - Biocompatibility and inflammatory response of silver tungstate, silver
85166672028&doi=10.3389%2ffbioe.2023.1215438&partnerID=40&md5=760aff0331649041ec97c
8b52357aec0
VL - 11
ID - 4995
ER -
TY - JOUR
AB - Silver tungstate (alpha-Ag2WO4), silver molybdate (beta-Ag2MoO4), and silver
vanadate (alpha-AgVO3) microcrystals have shown interesting antimicrobial
properties. However, their biocompatibility is not yet fully understood.
Cytotoxicity and the inflammatory response of silver-containing microcrystals were
analyzed in THP-1 and THP-1 differentiated as macrophage-like cells, with the
alamarBlue (TM) assay, flow cytometry, confocal microscopy, and ELISA. The present
investigation also evaluated redox signaling and the production of cytokines (TNF
alpha, IL-1 beta, IL-6, and IL-8) and matrix metalloproteinases (MMP-8 and -9). The
results showed that alpha-AgVO3 (3.9 mu g/mL) did not affect cell viability (p >
0.05). alpha-Ag2WO4 (7.81 mu g/mL), beta-Ag2MoO4 (15.62 mu g/mL), and alpha-AgVO3
(15.62 mu g/mL) slightly decreased cell viability (p <= 0.003). All silver-
containing microcrystals induced the production of O-2(-) and this effect was
mitigated by Reactive Oxygen Species (ROS) scavenger and N-acetylcysteine (NAC).
TNF alpha, IL-6 and IL-1 beta were not detected in THP-1 cells, while their
production was either lower (p <= 0.0321) or similar to the control group (p >=
0.1048) for macrophage-like cells. The production of IL-8 by both cellular
phenotypes was similar to the control group (p >= 0.3570). The release of MMP-8 was
not detected in any condition in THP-1 cells. Although MMP-9 was released by THP-1
cells exposed to alpha-AgVO3 (3.9 mu g/mL), no significant difference was found
with control (p = 0.7). Regarding macrophage-like cells, the release of MMP-8 and -
9 decreased in the presence of all microcrystals (p <= 0.010). Overall, the present
work shows a promising biocompatibility profile of, alpha-Ag2WO4, beta-Ag2MoO4, and
alpha-AgVO3 microcrystals.
AN - WOS:001042148600001
AU - Pimentel, Bnad
AU - De Annunzio, S. R.
AU - Assis, M.
AU - Barbugli, P. A.
AU - Longo, E.
AU - Vergani, C. E.
C7 - 1215438
DA - JUL 20
DO - 10.3389/fbioe.2023.1215438
PY - 2023
SN - 2296-4185
ST - Biocompatibility and inflammatory response of silver tungstate, silver
TI - Biocompatibility and inflammatory response of silver tungstate, silver
VL - 11
ID - 6000
ER -
TY - JOUR
AB - Continuously increasing application of silver nanoparticles (AgNPs) requires
information on their safety and performance under biological conditions. Assessment
of AgNPs in biological systems is also related to availability of robust
toxicological methods for evaluation of toxic potential of AgNPs and information on
their physicochemical state. Silver nanoparticles were subjected to action of
simulated saliva, gastric and intestinal fluids, appropriately supplemented with
digestive enzymes pepsin or pancreatin. The behaviour of AgNPs was determined using
dynamic light scattering and transmission electron microscopy, and their toxicity
as well as capability to induce inflammatory reactions were assessed using
reconstructed human tissue models (EpiOral, EpiGingival, EpiIntestinal). The study
revealed that during exposure to the fluids, AgNPs size and morphology changed and
depended on composition and pH of the respective fluid. If present, the change in
terms of growth of AgNPs size occurred immediately after contact of AgNPs with the
respective fluid and continued with prolonged time of contact. A pilot study on
reconstituted human tissue models revealed low toxicity and inflammatory effects of
AgNPs and confirmed the suitability of 3-D models for toxicological studies
including bioavailability. © 2017
AU - Pinďáková, L.
AU - Kašpárková, V.
AU - Kejlová, K.
AU - Dvořáková, M.
AU - Krsek, D.
AU - Jírová, D.
AU - Kašparová, L.
DB - Scopus
DO - 10.1016/j.ijpharm.2017.05.026
IS - 1-2
KW - Dynamic light scattering
Reconstructed human tissue models
Saliva
Simulated gastrointestinal fluids
Gastric Juice
Humans
Metal Nanoparticles
Particle Size
Pilot Projects
Silver
Tissue Culture Techniques
pancreatin
pepsin A
silver nanoparticle
metal nanoparticle
silver
absorption
Article
controlled study
human
human tissue
intestinal availability
intestine absorption
intestine fluid
particle size
priority journal
risk assessment
saliva
chemistry
pilot study
stomach juice
tissue culture technique
M3 - Article
PY - 2017
SP - 12-20
ST - Behaviour of silver nanoparticles in simulated saliva and gastrointestinal
fluids
TI - Behaviour of silver nanoparticles in simulated saliva and gastrointestinal
fluids
85019859650&doi=10.1016%2fj.ijpharm.2017.05.026&partnerID=40&md5=ed4d1863ef64f3773e
9ce938ee540e14
VL - 527
ID - 5471
ER -
TY - JOUR
AB - Continuously increasing application of silver nanoparticles (AgNPs) requires
information on their safety and performance under biological conditions. Assessment
of AgNPs in biological systems is also related to availability of robust
toxicological methods for evaluation of toxic potential of AgNPs and information on
their physicochemical state. Silver nanoparticles were subjected to action of
simulated saliva, gastric and intestinal fluids, appropriately supplemented with
digestive enzymes pepsin or pancreatin. The behaviour of AgNPs was determined using
dynamic light scattering and transmission electron microscopy, and their toxicity
as well as capability to induce inflammatory reactions were assessed using
reconstructed human tissue models (EpiOral, EpiGingival, EpiIntestinal). The study
revealed that during exposure to the fluids, AgNPs size and morphology changed and
depended on composition and pH of the respective fluid. If present, the change in
terms of growth of AgNPs size occurred immediately after contact of AgNPs with the
respective fluid and continued with prolonged time of contact. A pilot study on
reconstituted human tissue models revealed low toxicity and inflammatory effects of
AgNPs and confirmed the suitability of 3-D models for toxicological studies
including bioavailability. (C) 2017 Elsevier B.V. All rights reserved.
AN - WOS:000404505400002
AU - Pindkaova, L.
AU - Kasparkova, V.
AU - Kejlova, K.
AU - Dvorakova, M.
AU - Krsek, D.
AU - Jirova, D.
AU - Kasparova, L.
DA - JUL 15
DO - 10.1016/j.ijpharm.2017.05.026
IS - 1-2
PY - 2017
SN - 0378-5173
1873-3476
SP - 12-20
ST - Behaviour of silver nanoparticles in simulated saliva and gastrointestinal
fluids
T2 - INTERNATIONAL JOURNAL OF PHARMACEUTICS
TI - Behaviour of silver nanoparticles in simulated saliva and gastrointestinal
fluids
VL - 527
ID - 5917
ER -
TY - JOUR
AB - The rapid development of nanotechnologies and increased production and use of
nanomaterials raise concerns about their potential toxic effects for human health
and environment. To evaluate the biological effects of nanomaterials, a set of
reliable and reproducible methods and development of standard operating procedures
(SOPs) is required. In the framework of the European FP7 NanoValid project, three
different cell viability assays (MTS, ATP content, and caspase-3/7 activity) with
different readouts (absorbance, luminescence and fluorescence) and two immune
assays (ELISA of pro-inflammatory cytokines IL1-β and TNF-α) were evaluated by
inter-laboratory comparison. The aim was to determine the suitability and
reliability of these assays for nanosafety assessment. Studies on silver and copper
oxide nanoparticles (NPs) were performed, and SOPs for particle handling, cell
culture, and in vitro assays were established or adapted. These SOPs give precise
descriptions of assay procedures, cell culture/seeding conditions, NPs/positive
control preparation and dilutions, experimental well plate preparation, and
evaluation of NPs interference. The following conclusions can be highlighted from
the pan-European inter-laboratory studies: Testing of NPs interference with the
toxicity assays should always be conducted. Interference tests should be designed
as close as possible to the cell exposure conditions. ATP and MTS assays gave
consistent toxicity results with low inter-laboratory variability using Ag and CuO
NPs and different cell lines and therefore, could be recommended for further
validation and standardization. High inter-laboratory variability was observed for
Caspase 3/7 assay and ELISA for IL1-β and TNF-α measurements. © 2016, Springer-
Verlag Berlin Heidelberg.
AU - Piret, J. P.
AU - Bondarenko, O. M.
AU - Boyles, M. S. P.
AU - Himly, M.
AU - Ribeiro, A. R.
AU - Benetti, F.
AU - Smal, C.
AU - Lima, B.
AU - Potthoff, A.
AU - Simion, M.
AU - Dumortier, E.
AU - Leite, P. E. C.
AU - Balottin, L. B.
AU - Granjeiro, J. M.
AU - Ivask, A.
AU - Kahru, A.
AU - Radauer-Preiml, I.
AU - Tischler, U.
AU - Duschl, A.
AU - Saout, C.
AU - Anguissola, S.
AU - Haase, A.
AU - Jacobs, A.
AU - Nelissen, I.
AU - Misra, S. K.
AU - Toussaint, O.
DB - Scopus
DO - 10.1007/s00204-016-1897-2
IS - 6
KW - In vitro assays
Inter-laboratory studies
Interference
Nanoparticles
Standard operating procedures
Biological Assay
Cell Line, Tumor
Cell Survival
Copper
Cytokines
Europe
Humans
Laboratories
Metal Nanoparticles
Particle Size
Silver
Surface Properties
Toxicity Tests
caspase 3
caspase 7
interleukin 1beta
silver nanoparticle
copper
cupric oxide
cytokine
metal nanoparticle
silver
A-549 cell line
apoptosis
Article
atp content assay
biological activity
Caco-2 cell line
caspase 3 7 activity assay
clinical laboratory
controlled study
culture medium
cytotoxicity
evaluation study
fluorescence
Hep-G2 cell line
human
human cell
hydrodynamics
in vitro study
inflammation
light absorption
luminescence
materials handling
monocyte
MTS assay
nanotoxicology
particle size
priority journal
quality control procedures
reliability
reproducibility
standard operating procedure
toxicity assay
toxicity testing
bioassay
cell survival
chemistry
drug effects
laboratory
procedures
secretion (process)
standards
surface property
tumor cell line
M3 - Article
PY - 2017
SP - 2315-2330
ST - Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and
pro-inflammation assays for nanoparticles
TI - Pan-European inter-laboratory studies on a panel of in vitro cytotoxicity and
pro-inflammation assays for nanoparticles
85006355458&doi=10.1007%2fs00204-016-1897-
2&partnerID=40&md5=b59eead0e795f13916d55f39b70d395d
VL - 91
ID - 5509
ER -
TY - JOUR
AB - Objective. To evaluate the tissue responses to implants of Ketac Silver and
Super EBA cement in the guinea pig mandible. Study design. Sixteen guinea pigs were
used for 2 experimental periods of 4 and 12 weeks. Both materials were placed in
Teflon applicators and implanted into surgically prepared sites in the mandible. A
histologic examination for reaction to the material occurred after the animals were
killed and processed. Results. After 4 weeks, minimal inflammatory reactions were
observed in Ketac Silver implants, whereas the Super EBA implants showed minimal to
moderate inflammation. Localized foreign body reaction was present in areas of
fragmented small particles of Ketac Silver. At 12-weeks observation, no
inflammatory reactions were present around either material. Direct bone contact was
observed in 1 Ketac Silver implant. Conclusions. Ketac Silver and Super EBA cement
elicited mild reactions under the conditions of this model. From a biologic point
of view, these 2 materials may offer equal utility in endodontic surgery.
AN - WOS:000087075100020
AU - Pissiotis, E.
AU - Spangberg, L.
DA - MAY
DO - 10.1067/moe.2000.105173
IS - 5
PY - 2000
SN - 1079-2104
1528-395X
SP - 623-629
ST - Reaction of bony tissue to implanted silver glass ionomer and a reinforced
zinc oxide-eugenol cement
T2 - ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
TI - Reaction of bony tissue to implanted silver glass ionomer and a reinforced
zinc oxide-eugenol cement
VL - 89
ID - 6330
ER -
TY - JOUR
AB - Oral mucositis is a distressing toxic effect of systemic chemotherapy with
many commonly utilized drugs and of head and neck irradiation in patients with
cancer. The agents and methods that have been used and studied in chemotherapy- and
radiotherapy-induced oral mucositis, their mechanisms of action, and the current
knowledge of their efficiency to reduce the incidence, severity or shorten the
duration of oral mucositis are reviewed in this article. Oral cooling is a cheap
and available method to lower the severity of bolus 5-fluorouracil-induced oral
mucositis. However, more effective methods are needed. Results of studies with
granulocyte-macrophage colony-stimulating factor or granulocyte colony-stimulating
factor are promising. Lasers are partly beneficial, but equipment-demanding.
Modification of the chemotherapy regimen resulting in shortening of the exposition
time to chemotherapy agents or chronomodulation of chemotherapy has been shown to
lower mucosal toxicity of some regimens. Results of animal studies with locally
applied transforming growth factor β3 and interleukin-11 are also promising. Based
on the findings of the role of the inflammatory cascade in the response of normal
tissues to chemotherapy and radiotherapy, anti-inflammatory drugs might be
beneficial. At the present time, no agent has been shown to be uniformly
efficacious and can be accepted as standard therapy of chemotherapy- and
radiotherapy-induced oral mucositis. Further intensive research is needed.
AU - Plevová, P.
DB - Scopus
DO - 10.1016/S1368-8375(99)00033-0
IS - 5
KW - Antineoplastic agents
Chemically induced
Radiotherapy-adverse effects
Stomatitis control
Stomatitis prevention
Humans
Oral Hygiene
Radiation Injuries
Radiotherapy
Stomatitis
allopurinol
alpha tocopherol
atropine
azelastine
benzydamine
beta carotene
betamethasone
chamomile
cytokine
fluorouracil
folinate calcium
hydrogen peroxide
icosanoid
immunoglobulin g
indometacin
interleukin 11
kaolin pectin
melphalan
mesalazine
methotrexate
pentoxifylline
povidone iodine
propantheline bromide
retinoic acid
silver nitrate
sucralfate
unindexed drug
cancer
chemotherapy
clinical trial
cryotherapy
dental surgery
double blind procedure
helium neon laser
human
mouth hygiene
mucosa inflammation
nonhuman
priority journal
prophylaxis
review
stomatitis
M3 - Review
PY - 1999
SP - 453-470
ST - Prevention and treatment of chemotherapy- and radiotherapy-induced oral
mucositis: A review
T2 - Oral Oncology
TI - Prevention and treatment of chemotherapy- and radiotherapy-induced oral
mucositis: A review
0032766953&doi=10.1016%2fS1368-8375%2899%2900033-
0&partnerID=40&md5=38fe71b49f3a6c0312fdc449607c4efc
VL - 35
ID - 5784
ER -
TY - JOUR
AB - Background: Poplar leaf-buds (Populi gemmae) are used traditionally as anti-
inflammatory agents to the treatment of skin injuries or cough. They differ in
their diverse chemical composition and different types of activities, whose
mechanisms are not fully recognized. Purpose: Evaluation and comparison of anti-
inflammatory activity of leaf-buds extracts from Populus nigra, P. x berolinensis
and P. lasiocarpa and flavanones - pinocembrin and pinostrobin towards human
gingival fibroblasts (HGF-1) pro-inflammatory stimulated by silver nanoparticles
(AgNPs). Determination of antioxidant activity associated with anti-inflammatory
properties by means of bioautographic TLC tests. Methods: Phytochemical analysis
was performed by TLC and videodensitometry analysis. The extracts were standardized
on the pinocembrin and pinostrobin content. Bioautography was performed using 2,2-
diphenyl-1-picrylhydrazyl (DPPH) and riboflavin-light blue tetrazolium chloride
(riboflavin-light-NBT) radicals to assess the extracts and both flavanones radical
scavenging properties as well as potential inhibition of xanthine oxidase (XO)
activity. The protective effects of poplar buds extract and flavanones -
pinocembrin and pinostrobin on HGF-1 line exposured to AgNPs were investigated by
analysis of interleukin 6 (IL-6) and interleukin IL-1 beta (IL-1 beta) level
measured by ELISA kit. The messenger ribonucleic acid (mRNA) of both cytokines was
determined by real-time quantitative PCR. The involvement of cyclooxygenase 2
protein (COX-2) was studied using Western blot analysis. Results: The presence of
several flavanones and phenolic acids, which have radical scavenging properties,
was revealed in all of the bud poplar extracts analyzed. Treatment with particular
flavanones or extracts from buds of P. x berolinensis and P. nigra decreased the
IL-6 and IL-1 beta release in HGF-1 cells and down-regulation of mRNA for both
cytokines was observed. The COX-2 protein expression was demonstrated for
pinocembrin and P. x berolinensis buds. These effects were not observed for buds
from P. lasiocarpa not containing of flavonoids. Conclusion: The potential
protective role of pinocembrin and pinostrobin and extracts from buds P. nigra and
P. x berolinensis against AgNPs induced inflammation and cytotoxicity in HGF-1
cells is disclosed. In addition, the antioxidant properties of poplar bud extracts
have been demonstrated. P. x berolinensis buds showed the highest activity in both
the in vitro model and in the bioautographic tests.
AN - WOS:000463528800001
AU - Poblocka-Olech, L.
AU - Krauze-Baranowska, M.
DA - MAR 15
DO - 10.1016/j.phymed.2018.08.015
PY - 2019
SN - 0944-7113
SP - 1-9
ST - Anti-inflammatory and antioxidative effects of the buds from different
species of Populus in human gingival fibroblast cells: Role of bioflavanones
T2 - PHYTOMEDICINE
TI - Anti-inflammatory and antioxidative effects of the buds from different
VL - 56
ID - 5886
ER -
TY - JOUR
AB - Background: Poplar leaf-buds (Populi gemmae) are used traditionally as anti-
inflammatory agents to the treatment of skin injuries or cough. They differ in
their diverse chemical composition and different types of activities, whose
mechanisms are not fully recognized. Purpose: Evaluation and comparison of anti-
inflammatory activity of leaf-buds extracts from Populus nigra, P. × berolinensis
and P. lasiocarpa and flavanones - pinocembrin and pinostrobin towards human
gingival fibroblasts (HGF-1) pro-inflammatory stimulated by silver nanoparticles
(AgNPs). Determination of antioxidant activity associated with anti-inflammatory
properties by means of bioautographic TLC tests. Methods: Phytochemical analysis
was performed by TLC and videodensitometry analysis. The extracts were standardized
on the pinocembrin and pinostrobin content. Bioautography was performed using 2,2-
diphenyl-1-picrylhydrazyl (DPPH) and riboflavin-light blue tetrazolium chloride
(riboflavin-light-NBT) radicals to assess the extracts and both flavanones radical
scavenging properties as well as potential inhibition of xanthine oxidase (XO)
activity. The protective effects of poplar buds extract and flavanones –
pinocembrin and pinostrobin on HGF-1 line exposured to AgNPs were investigated by
analysis of interleukin 6 (IL-6) and interleukin IL-1β (IL-1β) level measured by
ELISA kit. The messenger ribonucleic acid (mRNA) of both cytokines was determined
by real-time quantitative PCR. The involvement of cyclooxygenase 2 protein (COX-2)
was studied using Western blot analysis. Results: The presence of several
flavanones and phenolic acids, which have radical scavenging properties, was
revealed in all of the bud poplar extracts analyzed. Treatment with particular
flavanones or extracts from buds of P. × berolinensis and P. nigra decreased the
IL-6 and IL-1β release in HGF-1 cells and down-regulation of mRNA for both
cytokines was observed. The COX-2 protein expression was demonstrated for
pinocembrin and P. × berolinensis buds. These effects were not observed for buds
from P. lasiocarpa not containing of flavonoids. Conclusion: The potential
protective role of pinocembrin and pinostrobin and extracts from buds P. nigra and
P. × berolinensis against AgNPs induced inflammation and cytotoxicity in HGF-1
cells is disclosed. In addition, the antioxidant properties of poplar bud extracts
have been demonstrated. P. × berolinensis buds showed the highest activity in both
the in vitro model and in the bioautographic tests. © 2018
AU - Pobłocka-Olech, L.
AU - Krauze-Baranowska, M.
DB - Scopus
DO - 10.1016/j.phymed.2018.08.015
KW - Anti-inflammatory effects
Pinocembrin
Pinostrobin
Poplar buds
TLC bioautography
Antioxidants
Cell Line
Cyclooxygenase 2
Cytokines
Fibroblasts
Flavanones
Gingiva
Gingivitis
Humans
Metal Nanoparticles
Plant Extracts
Plant Leaves
Populus
Silver
allopurinol
antioxidant
beta actin
caffeic acid
cyclooxygenase 2
flavanone derivative
interleukin 1beta
interleukin 6
messenger RNA
phenol derivative
phytochemical
pinocembrine
pinostrobin
plant extract
Populus berolinensis extract
Populus lasiocarpa extract
Populus nigra extract
protein
riboflavin
silver nanoparticle
taxifolin
unclassified drug
xanthine oxidase
xanthine oxidase inhibitor
cytokine
metal nanoparticle
Ptgs2 protein, mouse
silver
Article
bud
comparative effectiveness
controlled study
cytokine release
cytotoxicity
down regulation
enzyme activity
enzyme inhibition
ethnopharmacology
evaluation study
gingivitis
HGF-1 cell line
human
human cell
in vitro study
inflammation
nitroblue tetrazolium test
nonhuman
phytochemistry
Populus berolinensis
Populus lasiocarpa
Populus nigra
priority journal
thin layer chromatography
videodensitometry
Western blotting
cell line
chemically induced
chemistry
cytology
drug effect
fibroblast
genetics
gingiva
metabolism
plant leaf
preclinical study
procedures
M3 - Article
PY - 2019
SP - 1-9
ST - Anti-inflammatory and antioxidative effects of the buds from different
T2 - Phytomedicine
TI - Anti-inflammatory and antioxidative effects of the buds from different
85056155335&doi=10.1016%2fj.phymed.2018.08.015&partnerID=40&md5=e2348485e72138e757c
efa02dc8201f1
VL - 56
ID - 5425
ER -
TY - JOUR
AB - Oxidative stress, caused by reactive oxygen species (ROS), is a major
contributor to inflammatory bowel disease (IBD)-associated neoplasia. We mimicked
ROS exposure of the epithelium in IBD using non-tumour human colonic epithelial
cells (HCEC) and hydrogen peroxide (H2O2). A population of HCEC survived H2O2-
induced oxidative stress via JNK-dependent cell cycle arrests. Caspases, p21(WAF1)
and gamma-H2AX were identified as JNK-regulated proteins. Up-regulation of caspases
was linked to cell survival and not, as expected, to apoptosis. Inhibition using
the pan-caspase inhibitor Z-VAD-FMK caused up-regulation of gamma-H2AX, a DNA-
damage sensor, indicating its negative regulation via caspases. Cell cycle analysis
revealed an accumulation of HCEC in the G(1)-phase as first response to oxidative
stress and increased S-phase population and then apoptosis as second response
following caspase inhibition. (1)- and S-phase by overriding the G(1)/S- and intra-
S checkpoints despite DNA-damage. (2)/M-phase and decreased apoptosis. Caspases
mediate survival of oxidatively damaged HCEC via gamma-H2AX suppression, although
its direct proteolytic inactivation was excluded. Conversely, we found that
oxidative stress led to caspase-dependent proteolytic degradation of the DNA-damage
checkpoint protein A that is upstream of gamma-H2AX. As a consequence, undetected
DNA-damage and increased proliferation were found in repeatedly H2O2-exposed HCEC.
Such features have been associated with neoplastic transformation and appear here
to be mediated by a non-apoptotic function of caspases. Overexpression of upstream
p-JNK in active ulcerative colitis also suggests a potential importance of this
pathway in vivo.
AN - WOS:000322202300011
AU - Poehlmann, A.
AU - Reissig, K.
AU - Just, A.
AU - Walluscheck, D.
AU - Hartig, R.
AU - Schinlauer, A.
AU - Lessel, W.
AU - Guenther, T.
AU - Silver, A.
AU - Steinberg, P.
AU - Roessner, A.
DA - JUL
DO - 10.1111/jcmm.12079
IS - 7
PY - 2013
SN - 1582-1838
1582-4934
SP - 901-913
ST - Non-apoptotic function of caspases in a cellular model of hydrogen peroxide-
associated colitis
T2 - JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
TI - Non-apoptotic function of caspases in a cellular model of hydrogen peroxide-
associated colitis
VL - 17
ID - 6718
ER -
TY - JOUR
AB - Abstract: The influence of size of nanoparticles (NP), especially in regard
to pulmonary toxicity, has been widely investigated. In general, NP with smaller
diameters are more pro-inflammatory in vivo, at least in terms of neutrophil
influx. Nevertheless, the influence of size of NP on polymorphonuclear neutrophil
(PMN) cell biology is poorly documented. In the study here, it was decided to
determine if AgNP with a diameter of 70 nm (AgNP70) will alter the biology of human
PMN similarly to AgNP20 previously reported to induce apoptosis and inhibit de novo
protein synthesis. The results here indicated that, in contrast to AgNP20, AgNP70
delayed PMN apoptosis. However, both AgNP20 and AgNP70 inhibited de novo protein
synthesis. Both forms of AgNP did not significantly increase reactive oxygen
species (ROS) production, but AgNP20 significantly increased the cell production of
the CXCL8 chemokine (IL-8). In addition, AgNP20, but not AgNP70, induced the
release of albumin and matrix metalloproteinase-9 (MMP-9/gelatinase B) into culture
supernatants. Consistent with this latter observation, gelatinase activity was
increased by AgNP20, as assessed by zymography. From these outcomes, it is
concluded that two NP with different initial diameters can possess similar – as
well as distinct – biological properties in modulating human PMN functions. These
outcomes are testimony to the complexity of the modes of action of NP at the
cellular level. © 2015 Informa UK Limited trading as Taylor & Francis Group.
AU - Poirier, M.
AU - Simard, J. C.
AU - Girard, D.
DB - Scopus
DO - 10.3109/1547691X.2015.1106622
IS - 3
KW - apoptosis
inflammation
Nanosilver
nanotoxicology
neutrophils
Apoptosis
Cells, Cultured
Humans
Interleukin-8
Matrix Metalloproteinase 9
Metal Nanoparticles
Neutrophils
Particle Size
Silver
gelatinase B
interleukin 8
silver nanoparticle
metal nanoparticle
MMP9 protein, human
silver
Article
cell shape
cell viability
controlled study
cytokine production
cytokine release
cytology
cytotoxicity
degranulation
enzyme activity
human
human cell
immunobiology
neutrophil
priority journal
protein synthesis
zeta potential
zymography
cell culture
chemistry
drug effects
metabolism
particle size
physiology
M3 - Article
PY - 2016
SP - 375-385
ST - Silver nanoparticles of 70 nm and 20 nm affect differently the biology of
human neutrophils
TI - Silver nanoparticles of 70 nm and 20 nm affect differently the biology of
human neutrophils
84948739123&doi=10.3109%2f1547691X.2015.1106622&partnerID=40&md5=b2126d7fe7cfa20acd
76a2229631819f
VL - 13
ID - 5496
ER -
TY - JOUR
AB - The influence of size of nanoparticles (NP), especially in regard to
pulmonary toxicity, has been widely investigated. In general, NP with smaller
diameters are more pro-inflammatory in vivo, at least in terms of neutrophil
influx. Nevertheless, the influence of size of NP on polymorphonuclear neutrophil
(PMN) cell biology is poorly documented. In the study here, it was decided to
determine if AgNP with a diameter of 70nm (AgNP70) will alter the biology of human
PMN similarly to AgNP20 previously reported to induce apoptosis and inhibit de novo
protein synthesis. The results here indicated that, in contrast to AgNP20, AgNP70
delayed PMN apoptosis. However, both AgNP20 and AgNP70 inhibited de novo protein
synthesis. Both forms of AgNP did not significantly increase reactive oxygen
species (ROS) production, but AgNP20 significantly increased the cell production of
the CXCL8 chemokine (IL-8). In addition, AgNP20, but not AgNP70, induced the
release of albumin and matrix metalloproteinase-9 (MMP-9/gelatinase B) into culture
supernatants. Consistent with this latter observation, gelatinase activity was
increased by AgNP20, as assessed by zymography. From these outcomes, it is
concluded that two NP with different initial diameters can possess similar - as
well as distinct - biological properties in modulating human PMN functions. These
outcomes are testimony to the complexity of the modes of action of NP at the
cellular level.
AN - WOS:000378925500010
AU - Poirier, M.
AU - Simard, J. C.
AU - Girard, D.
DA - MAY
DO - 10.3109/1547691X.2015.1106622
IS - 3
PY - 2016
SN - 1547-691X
1547-6901
SP - 375-385
ST - Silver nanoparticles of 70nm and 20nm affect differently the biology of human
neutrophils
TI - Silver nanoparticles of 70nm and 20nm affect differently the biology of human
neutrophils
VL - 13
ID - 6180
ER -
TY - JOUR
AB - Because of their antimicrobial properties, silver nanoparticles are
increasingly incorporated in food-related and hygiene products, which thereby could
lead to their ingestion. Although their cytotoxicity mediated by oxidative stress
has been largely studied, their effects on inflammation remain controversial.
Moreover, the involvement of silver ions (originating from Ag0 oxidation) in their
mode of action is still unclear. In this context, the present study aims at
assessing the impact of silver nanoparticles on the secretion of the pro-
inflammatory chemokine interleukin-8 by Caco-2 cells forming an in vitro model of
the intestinal mucosal barrier. Silver nanoparticles induced a vectorized secretion
of interleukin-8 towards the apical compartment, which is found in the medium 21 h
after the incubation. This secretion seems mediated by Nrf2 signalling pathway that
orchestrates cellular defense against oxidative stress. The soluble silver fraction
of silver nanoparticles suspensions led to a similar amount of secreted
interleukin-8 than silver nanoparticles, suggesting an involvement of silver ions
in this interleukin-8 secretion. © 2020 Elsevier B.V.
AU - Polet, M.
AU - Laloux, L.
AU - Cambier, S.
AU - Ziebel, J.
AU - Gutleb, A. C.
DB - Scopus
DO - 10.1016/j.toxlet.2020.02.004
KW - Caco-2 cells
In vitro
Interleukin-8
Intestinal inflammation
Caco-2 Cells
Cell Survival
Colon
Enteritis
Gene Expression
Humans
Inflammation
Metal Nanoparticles
Oxidative Stress
Particle Size
Silver
interleukin 8
silver
silver nanoparticle
metal nanoparticle
Nfe2l2 protein, mouse
Article
Caco-2 cell line
human
human cell
intestine mucosa
oxidative stress
priority journal
signal transduction
cell survival
colon
drug effect
enteritis
gene expression
inflammation
metabolism
particle size
pathology
M3 - Article
PY - 2020
SP - 14-24
ST - Soluble silver ions from silver nanoparticles induce a polarised secretion of
interleukin-8 in differentiated Caco-2 cells
T2 - Toxicology Letters
TI - Soluble silver ions from silver nanoparticles induce a polarised secretion of
interleukin-8 in differentiated Caco-2 cells
85079840267&doi=10.1016%2fj.toxlet.2020.02.004&partnerID=40&md5=43051bb3f3f6cfcec42
4637ed9dacc31
VL - 325
ID - 5325
ER -
TY - JOUR
AB - Nano-sized metal oxides are currently the most manufactured nanomaterials
(NMs), and are increasingly used in consumer products. Recent exposure data reveal
a genuine potential for adverse health outcomes for a vast array of NMs, however
the underlying mechanisms are not fully understood. To elucidate size-related
molecular effects, differentiated THP-1 cells were exposed to nano-sized materials
(n-TiO2, n-ZnO and n-Ag), or their bulk-sized (b-ZnO and b-TiO2) or ionic (i-Ag)
counterparts, and genome-wide gene expression changes were studied at low-toxic
concentrations (<15% cytotoxicity). TiO2 materials were nontoxic in MTT assay,
inducing only minor transcriptional changes. ZnO and Ag elicited dose-dependent
cytotoxicity, wherein ionic and particulate effects were synergistic with respect
to n-ZnO-induced cytotoxicity. In gene expression analyzes, 6h and 24h samples
formed two separate hierarchical clusters. N-ZnO and n-Ag shared only 3.1% and
24.6% differentially expressed genes (DEGs) when compared to corresponding control.
All particles, except TiO2, activated various metallothioneins. At 6h, n-Zn, b-Zn
and n-Ag induced various immunity related genes associating to pattern recognition
(including toll-like receptor), macrophage maturation, inflammatory response (TNF
and IL-1beta), chemotaxis (CXCL8) and leucocyte migration (CXCL2-3 and CXCL14).
After 24h exposure, especially n-Ag induced the expression of genes related to
virus recognition and type I interferon responses. These results strongly suggest
that in addition to ionic effects mediated by metallothioneins, n-Zn and n-Ag
induce expression of genes involved in several innate and adaptive immunity
associated pathways, which are known to play crucial role in immuno-regulation.
This raises the concern of safe use of metal oxide and metal nanoparticle products,
and their biological effects.
AN - WOS:000415921500008
AU - Poon, W. L.
AU - Alenius, H.
AU - Ndika, J.
AU - Fortino, V.
AU - Kolhinen, V.
AU - Mesceriakovas, A.
AU - Wang, M. F.
AU - Greco, D.
AU - Lahde, A.
AU - Jokiniemi, J.
AU - Lee, J. C. Y.
AU - El-Nezami, H.
AU - Karisola, P.
DO - 10.1080/17435390.2017.1382600
IS - 7
PY - 2017
SN - 1743-5390
1743-5404
SP - 936-951
ST - Nano-sized zinc oxide and silver, but not titanium dioxide, induce innate and
adaptive immunity and antiviral response in differentiated THP-1 cells
T2 - NANOTOXICOLOGY
TI - Nano-sized zinc oxide and silver, but not titanium dioxide, induce innate and
VL - 11
ID - 6553
ER -
TY - JOUR
AB - Nano-sized metal oxides are currently the most manufactured nanomaterials
(NMs), and are increasingly used in consumer products. Recent exposure data reveal
a genuine potential for adverse health outcomes for a vast array of NMs, however
the underlying mechanisms are not fully understood. To elucidate size-related
molecular effects, differentiated THP-1 cells were exposed to nano-sized materials
(n-TiO2, n-ZnO and n-Ag), or their bulk-sized (b-ZnO and b-TiO2) or ionic (i-Ag)
counterparts, and genome-wide gene expression changes were studied at low-toxic
concentrations (<15% cytotoxicity). TiO2 materials were nontoxic in MTT assay,
inducing only minor transcriptional changes. ZnO and Ag elicited dose-dependent
cytotoxicity, wherein ionic and particulate effects were synergistic with respect
to n-ZnO-induced cytotoxicity. In gene expression analyzes, 6 h and 24 h samples
formed two separate hierarchical clusters. N-ZnO and n-Ag shared only 3.1% and
24.6% differentially expressed genes (DEGs) when compared to corresponding control.
All particles, except TiO2, activated various metallothioneins. At 6 h, n-Zn, b-Zn
and n-Ag induced various immunity related genes associating to pattern recognition
(including toll-like receptor), macrophage maturation, inflammatory response (TNF
and IL-1beta), chemotaxis (CXCL8) and leucocyte migration (CXCL2-3 and CXCL14).
After 24 h exposure, especially n-Ag induced the expression of genes related to
virus recognition and type I interferon responses. These results strongly suggest
that in addition to ionic effects mediated by metallothioneins, n-Zn and n-Ag
induce expression of genes involved in several innate and adaptive immunity
associated pathways, which are known to play crucial role in immuno-regulation.
This raises the concern of safe use of metal oxide and metal nanoparticle products,
and their biological effects. © 2017 Informa UK Limited, trading as Taylor &
Francis Group.
AU - Poon, W. L.
AU - Alenius, H.
AU - Ndika, J.
AU - Fortino, V.
AU - Kolhinen, V.
AU - Meščeriakovas, A.
AU - Wang, M.
AU - Greco, D.
AU - Lähde, A.
AU - Jokiniemi, J.
AU - Lee, J. C. Y.
AU - El-Nezami, H.
AU - Karisola, P.
DB - Scopus
DO - 10.1080/17435390.2017.1382600
IS - 7
KW - Ag
immune system
nanomaterials
transcriptomics
ZnO
Adaptive Immunity
Cell Survival
Gene Expression
Genome-Wide Association Study
Humans
Immunity, Innate
Macrophages
Metal Nanoparticles
Particle Size
Silver
THP-1 Cells
Time Factors
Titanium
Virus Diseases
Zinc Oxide
CXCL14 chemokine
CXCL2 chemokine
CXCL3 chemokine
interleukin 1beta
interleukin 8
messenger RNA
metallothionein
pattern recognition receptor
silver nanoparticle
toll like receptor
metal nanoparticle
silver
titanium
titanium dioxide
zinc oxide
adaptive immunity
Article
controlled study
cytotoxicity
genome
immunoregulation
inflammation
innate immunity
macrophage
microarray analysis
MTT assay
particle size
priority journal
THP-1 cell line
virus immunity
cell survival
dose response
drug effects
gene expression
genetics
genome-wide association study
human
immunology
time factor
virus infection
M3 - Article
PY - 2017
SP - 936-951
ST - Nano-sized zinc oxide and silver, but not titanium dioxide, induce innate and
T2 - Nanotoxicology
TI - Nano-sized zinc oxide and silver, but not titanium dioxide, induce innate and
85030179164&doi=10.1080%2f17435390.2017.1382600&partnerID=40&md5=bf65eb2176a246f622
885a9915e3df45
VL - 11
ID - 5539
ER -
TY - JOUR
AB - Bioactive, oxygenated metabolites of polyunsaturated fatty acids (PUFAs) are
important indicators of inflammation and oxidative stress but almost nothing is
known about their interactions with nanomaterials (NMs). To investigate the effects
of nano-sized materials (n-TiO2, n-ZnO, n-Ag) and their bulk-sized or ionic (b-
TiO2, b-ZnO, i-Ag) counterpart, we studied the status of oxidative stress and PUFA
metabolism in THP-1 cells at low-toxic concentrations (<15% cytotoxicity) 6 h or 24
h after the particle exposures by LC/MS and microarray. N-Ag had a significant and
sustained impact on cellular antioxidant defense, seen as incremental synthesis and
accumulation of glutathione (GSH) in the cell, and reduction of superoxide
dismutase (SOD) activity. The cellular particle doses were largely dependent on
exposure duration and particle dissolution, and active transporter mechanisms
controlled the concentration of Zn in cytosol. Even at these sub-toxic
concentrations, n-Ag was able to induce statistically significant elevation in the
5-HETE: arachidonic acid ratio at 24 h, which suggests association to oxidative
stress and induction of pro-inflammatory responses. This was supported by the
enhanced gene expression of chemotaxis-related genes. Overall, THP-1 cells
internalized all tested particles, but only n-Ag led to low level of oxidative
stress through ROS production and antioxidant balance disruption. N-Ag stimulated
arachidonic acid oxidation to form 5-HETE which further magnified the inflammatory
responses by enhancing the production of mitochondrial superoxide and leukocyte
chemokines. Since the sustained n-Ag uptake was detected, the effects may last long
and function as a trigger for the low-grade inflammation playing role in the
chronic inflammatory diseases. © 2019, © 2019 Informa UK Limited, trading as Taylor
& Francis Group.
AU - Poon, W. L.
AU - Lee, J. C. Y.
AU - Leung, K. S.
AU - Alenius, H.
AU - El-Nezami, H.
AU - Karisola, P.
DB - Scopus
DO - 10.1080/17435390.2019.1687776
IS - 4
KW - fatty acid metabolism
LC/MS
metal oxides
microarray analysis
Nanoparticles
Antioxidants
Glutathione
Humans
Hydroxyeicosatetraenoic Acids
Inflammation
Metal Nanoparticles
Mitochondria
Oxidative Stress
Silver
THP-1 Cells
Titanium
Zinc Oxide
5 hydroxyicosatetraenoic acid
arachidonic acid
CXCL1 chemokine
CXCL2 chemokine
CXCL3 chemokine
glutathione
polyunsaturated fatty acid
silver nanoparticle
thymus and activation regulated chemokine
5-hydroxy-6,8,11,14-eicosatetraenoic acid
antioxidant
hydroxyicosatetraenoic acid
metal nanoparticle
silver
titanium
titanium dioxide
zinc oxide
Article
cell density
cell viability
chemotaxis
controlled study
cytokine production
cytosol
dispersion
dissolution
gene expression
human
human cell
inflammation
mitochondrion
monocyte
nanotoxicology
oxidative stress
phagocytosis
priority journal
protein content
T lymphocyte
THP-1 cell line
drug effect
immunology
metabolism
M3 - Article
PY - 2020
SP - 453-467
ST - Nanosized silver, but not titanium dioxide or zinc oxide, enhances oxidative
stress and inflammatory response by inducing 5-HETE activation in THP-1 cells
T2 - Nanotoxicology
TI - Nanosized silver, but not titanium dioxide or zinc oxide, enhances oxidative
stress and inflammatory response by inducing 5-HETE activation in THP-1 cells
85075734270&doi=10.1080%2f17435390.2019.1687776&partnerID=40&md5=6038176388151a6267
67b8bb541a13f7
VL - 14
ID - 5287
ER -
TY - JOUR
AB - Nanotechnology has led to the development of numerous new systems for drug
delivery into the target tissue, as well as novel methods that may be useful in the
treatment of liver fibrosis. Inorganic and organic nanoparticles (NPs) are
currently used in medical investigations and in the treatment of liver diseases,
with adverse reactions observed in some cases. A revised treatment procedure
involving NPs is necessary to develop future drug delivery systems having minimal
noxious effects on the hepatic cells that take up and metabolize these particles in
a different manner, in order to find the medication that is capable of blocking and
even reversing fibrosis in an inflamed liver. In addition, the administered
medication should not induce systemic responses against the NPs used in the
treatment. © 2020, Polish Physiological Society. All rights reserved.
AU - Pop, D. D. Z.
AU - Mitrea, D. R.
AU - Suciu, S.
AU - Clichici, S. V.
DB - Scopus
DO - 10.26402/jpp.2020.6.01
IS - 6
KW - Liver fibrosis
Nanoparticle delivery systems
Nanoparticles
Nanotherapy
Nonalcoholic fatty liver disease
Nonalcoholic steatohepatitis
Stellate cells
Therapeutic nanomaterials
actin
albumin
antioxidant loaded nanoparticle
calcium phosphate nanoparticle
capecitabine
carbon nanotube nanoparticle
carboxymethylcellulose docetaxel conjugated nanoparticle
cisplatin
collagen
curcumin
curcumin loaded nanoparticle
desmin
dexamethasone loaded liposome
doxorubicin
fullerene nanoparticle
glucose transporter
glycyrrhizic acid
gold nanoparticle
graphene nanoparticle
Hermes antigen
hyaluronic acid micelle
inorganic nanoparticle
interleukin 6
lipid nanoparticle
macrogol
manganese oxide nanoparticle
metal nanoparticle
microRNA loaded nanoparticle
nanoencapsulated carvacrol nanoparticle
nanomaterial
naringenin loaded nanoparticle
organic anionic transporter
organic nanoparticle
procollagen
quercetin loaded nanoparticle
resveratrol
resveratrol loaded nanoparticle
silibinin
silica oxide nanoparticle
silver nanoparticle
silymarin
silymarin encapsulated nanoparticle
silymarin loaded liposome
sodium dependent multivitamin transporter
Sorafenib loaded poly actic co glycolic acid nanoparticle
unclassified drug
virus vector
Vitamin A coupled liposome
Article
biocompatibility
biomineralization
blood brain barrier
epithelium cell
hepatic stellate cell
hepatitis
human
hydrophobicity
inflammatory cell
Kupffer cell
lipid peroxidation
liver fibrosis
long term care
macrophage
nanoemulsion
nanotechnology
nonhuman
oxidative stress
particle size
phagocytosis
pinocytosis
self microemulsifying drug delivery system
SH-SY5Y cell line
M3 - Article
PY - 2020
ST - Nanostructure-based therapies for liver fibrosis
T2 - Journal of Physiology and Pharmacology
TI - Nanostructure-based therapies for liver fibrosis
85103860053&doi=10.26402%2fjpp.2020.6.01&partnerID=40&md5=b1b84f6e9e92b2140a11f0605
c66ff82
VL - 71
ID - 5333
ER -
TY - JOUR
AB - Anecdotal reports in the press and epidemiological studies suggest that
deployment to Iraq and Afghanistan may be associated with respiratory diseases and
symptoms in U.S. military personnel and veterans. Exposures during military
operations were complex, but virtually all service members were exposed to high
levels of respirable, geogenic dust. Inhalation of other dusts has been shown to be
associated with adverse health effects, but the pulmonary toxicity of ambient dust
from Iraq has not been previously studied. The relative toxicity of Camp Victory
dust was evaluated by comparing it to particulate matter from northern Kuwait, a
standard U.S. urban dust, and crystalline silica using a single intratracheal
instillation in rats. Lung histology, protein levels, and cell counts were
evaluated in the bronchoalveolar lavage fluid 1-150 d later. The Iraq dust provoked
an early significant, acute inflammatory response. However, the level of
inflammation in response to the Iraq dust, U.S. urban dust, and Kuwait dust rapidly
declined and was nearly at control levels by the end of the study At later times,
animals exposed to the Iraq, U.S. urban, or Kuwait dusts showed increased small
airway remodeling and emphysema compared to silica-exposed and control animals
without evidence of fibrosis or premalignant changes. The severity and persistence
of pulmonary toxicity of these three dusts from the Middle East resemble those of a
U.S. urban dust and are less than those of silica. Therefore, Iraq dust exposure is
not highly toxic, but similar to other poorly soluble low-toxicity dusts.
AN - WOS:000366934000001
AU - Porter, K. L.
AU - Green, F. H. Y.
AU - Harley, R. A.
AU - Vallyathan, V.
AU - Castranova, V.
AU - Waldron, N. R.
AU - Leonard, S. S.
AU - Nelson, D. E.
AU - Lewis, J. A.
AU - Jackson, D. A.
DA - DEC 17
DO - 10.1080/15287394.2015.1072611
IS - 23-24
PY - 2015
SN - 1528-7394
1087-2620
SP - 1385-1408
ST - EVALUATION OF THE PULMONARY TOXICITY OF AMBIENT PARTICULATE MATTER FROM CAMP
VICTORY, IRAQ
T2 - JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
TI - EVALUATION OF THE PULMONARY TOXICITY OF AMBIENT PARTICULATE MATTER FROM CAMP
VICTORY, IRAQ
VL - 78
ID - 6579
ER -
TY - JOUR
AB - Metals enriched in ambient air fine particulate matter (PM2.5) are thought to
contribute to the pathogenesis of PM2.5-induced inflammatory lung diseases. An
important mechanism involved in metal-induced lung injury involves increased
oxidative stress due to generation of reactive oxygen species. The redox sensitive
transcription factor, nuclear factor kappa B (NF-kappa B) converts extracellular
oxidative stress signals into changes in expression of genes associated with
diverse cellular activities. The purpose of this study was to determine the
mechanism by which exposure to Fe or Se, at environmentally relevant
concentrations, leads to an increased release of chemokines by cultured human lung
epithelial cells (A549). We tested the hypothesis that NF-kappa B signaling pathway
is involved in the metal induced IL-8 and MCP-1 release by Fe and Se. Exposure to
Fe or Se induced an enhanced release of chemokines at 6 and 24 h, and mediated
nuclear translocation of NF-kappa B. Levels of chemokines in response to Fe were
significantly suppressed in the presence of BMS-345541, a specific inhibitor of NF-
kappa B. Similar effects were seen in response to Se, indicating the involvement of
NF-kappa B in the metal-induced chemokine release, while not affecting the AP-1 c-
Jun-DNA binding activity. Overall, results indicate that both Fe and Se, at ambient
levels, possess the potential for inducing lung inflammation via an oxidative
stress pathway in lung epithelial cells.
AN - WOS:000320272700004
AU - Potnis, P. A.
AU - Mitkus, R.
AU - Elnabawi, A.
AU - Squibb, K.
AU - Powell, J. L.
DO - 10.1039/c3tx50012h
IS - 4
PY - 2013
SN - 2045-452X
2045-4538
SP - 259-269
ST - Role of NF-kappa B in the oxidative stress-induced lung inflammatory response
to iron and selenium at ambient levels
TI - Role of NF-kappa B in the oxidative stress-induced lung inflammatory response
to iron and selenium at ambient levels
VL - 2
ID - 6705
ER -
TY - JOUR
AB - Increasing use of engineered nanomaterials (ENM) in consumer products and
commercial applications has helped drive a rise in research related to the
environmental health and safety (EHS) of these materials. Within the cacophony of
information on ENM EHS to date are data indicating that these materials may be
neurotoxic in adult animals. Evidence of elevated inflammatory responses, increased
oxidative stress levels, alterations in neuronal function, and changes in cell
morphology in adult animals suggests that ENM exposure during development could
elicit developmental neurotoxicity (DNT), especially considering the greater
vulnerability of the developing brain to some toxic insults. In this review, we
examine current findings related to developmental neurotoxic effects of ENM in the
context of identifying research gaps for future risk assessments. The basic risk
assessment paradigm is presented, with an emphasis on problem formulation and
assessments of exposure, hazard, and dose response for DNT. Limited evidence
suggests that in utero and postpartum exposures are possible, while fewer than 10
animal studies have evaluated DNT, with results indicating changes in synaptic
plasticity, gene expression, and neurobehavior. Based on the available information,
we use current testing guidelines to highlight research gaps that may inform ENM
research efforts to develop data for higher throughput methods and future risk
assessments for DNT. Although the available evidence is not strong enough to reach
conclusions about DNT risk from ENM exposure, the data indicate that consideration
of ENM developmental neurotoxic potential is warranted. © Published by Oxford
University Press on behalf of the Society of Toxicology 2013.
AU - Powers, C. M.
AU - Bale, A. S.
AU - Kraft, A. D.
AU - Makris, S. L.
AU - Trecki, J.
AU - Cowden, J.
AU - Hotchkiss, A.
AU - Gillespie, P. A.
DB - Scopus
DO - 10.1093/toxsci/kft109
IS - 2
KW - Developmental neurotoxicity
Risk assessment research
Animals
Humans
Nanostructures
Nervous System
Risk Assessment
developmental neurotoxicity
engineered nanomaterials
risk assessment research.
copper
iron
magnetite
manganese
nanomaterial
polystyrene
povidone
silver
article
behavior change
blood brain barrier
blood placenta barrier
brain weight
developmental toxicity
gene expression
growth
health hazard
hearing
human
in vitro study
in vivo study
inflammation
learning
memory
motor performance
nanoengineering
nerve cell plasticity
neuropathology
neurotoxicity
nonhuman
planning
practice guideline
prenatal exposure
puerperium
risk assessment
startle reflex
survival
toxicity testing
M3 - Article
PY - 2013
SP - 225-242
ST - Developmental neurotoxicity of engineered nanomaterials: Identifying research
needs to support human health risk assessment
TI - Developmental neurotoxicity of engineered nanomaterials: Identifying research
needs to support human health risk assessment
84880693748&doi=10.1093%2ftoxsci
%2fkft109&partnerID=40&md5=73e6f92110e4f75124825230563b1fb1
VL - 134
ID - 5707
ER -
TY - JOUR
AB - Nano silver particle (NSPs), is one of the most attractive nano materials,
and has been widely used in a range of biomedical applications, including
diagnosis, treatment, drug delivery, medical device coating, and for personal
health care. Silver nanoparticles can be synthesized by various methods but citrate
reduced and stabilized particles gain an advantage over other methods because of
its morphology, stable nature, cost effective and easy scale-up nature. In the
present study, the silver nanoparticle was synthesized by reducing silver salt with
sodium citrate that showed the characteristic peak at 410 nm by means of UV-Visible
spectroscopy. Antibacterial activity was performed against S.aureus and
P.aeruginosa, and S.aureusand was found to be more susceptible. Characterized
colloidal silver nanoparticles were used for the coating process. Coating on copper
plate was achieved by Dip method, and characterized using SEM, EDAX and FTIR.
Resulting EDAX and SEM images showed the morphology of the coated surface with
their elemental composition. FTIR interferogram showed the characteristic
functional groups at 1290.09 cm(-1) and 1483.68cm(-1) responsible for the reduction
of silver salt into silver nanoparticles. Antimicrobial activity was performed for
the coated metal against the Trichomonasvaginalis, a sexual transmitted pathogen
that attribute to pelvic inflammatory disease (PID).The inhibition diameter is of
about 31mm, thereby considering the fabricated nano composite to be effective
against the disease causing microbial species. Invitro cytotoxicity studies in the
Vero cell line show 81% viability with no significant morphological alterations.
Hence, the fabricated nano composite could be a future benchmark for implant
functionalization.
AN - WOS:000617380100017
AU - Prabasheela, B.
AU - Sakithya, V.
DA - DEC
DO - 10.22376/ijpbs/lpr.2020.10.5.L127-137
IS - 5
PY - 2020
SN - 2250-0480
SP - L127-L137
ST - Fabrication of Silver Nanocomposite on Copper Metal- A Potential Strategy to
Alleviate Pelvic Inflammatory Disease
T2 - INTERNATIONAL JOURNAL OF LIFE SCIENCE AND PHARMA RESEARCH
TI - Fabrication of Silver Nanocomposite on Copper Metal- A Potential Strategy to
Alleviate Pelvic Inflammatory Disease
VL - 10
ID - 6013
ER -
TY - JOUR
AB - Biocompatibility of medical grade polyurethane coated with polyaniline (PANi)
and polyaniline-silver nanoparticle composite (PANi-AgNp) is reported here. These
modified films showed 23 and 18% of 3T3 L1 cell death when compared to 41% with
virgin polyurethane (PU) after 48. h of incubation, respectively. All the surfaces
elucidated inflammatory response in the form of enhanced expressions of the
proinflammatory cytokines genes, TNF-α and IL-6. But these values were less (by
20%) on modified films than on the bare PU. Attachment of Pseudomonas and Bacillus
were markedly less on PANi-AgNp coated surface (by 90.6 and 50.5%, respectively)
when compared to the uncoated PU. As the CFU counts decreases on the nanoparticle
coated PU, the adsorbed carbohydrate as well as protein content on to the surface
of polymer decreases accordingly, indicating less attachment. A 20% reduction in
the thickness of biofilm was observed in PANi-AgNp coated PU surface. A very strong
positive correlation is observed between the contact angles of the polymers and the
various biological parameters (namely colony forming units, protein, carbohydrate,
cell death and inflammatory response), indicating hydrophilic surfaces prevent
bacterial biofilm as well as are compatible to cells when compared to hydrophobic
surfaces. Coating PU with PANi and PANi. +. AgNp renders the surface conductive,
suggesting potential application in electrochemical biosensors. In addition, these
modifications make the surface more biocompatible than the original PU. © 2011
Elsevier B.V.
AU - Prabhakar, P. K.
AU - Raj, S.
AU - Anuradha, P. R.
AU - Sawant, S. N.
AU - Doble, M.
DB - Scopus
DO - 10.1016/j.colsurfb.2011.03.033
IS - 1
KW - Biofilm
Nanocomposites
Polyaniline
Polyurethane
3T3-L1 Cells
Aniline Compounds
Animals
Biofilms
Cell Survival
Metal Nanoparticles
Mice
Polyurethanes
Silver
Surface Properties
Bacillus (bacterium)
Pseudomonas
Bacteriology
Biocompatibility
Biosensors
Carbohydrates
Cell death
Contact angle
Gene expression
Hydrophobicity
Nanoparticles
Plastic coatings
Polymers
Surface chemistry
carbohydrate
interleukin 6
polyaniline
polyurethan
silver nanoparticle
Bacterial biofilm
Biological parameter
Coated surface
Colony forming units
Electrochemical biosensor
Hydrophilic surfaces
Hydrophobic surfaces
Medical grades
Modified film
Polyurethane composites
Positive correlations
Potential applications
Proinflammatory cytokines
Protein contents
adipocyte
adsorption
animal cell
apoptosis
article
Bacillus subtilis
bacterium adherence
biofilm
cell interaction
cell strain 3T3
colony forming unit
controlled study
inflammation
material coating
mouse
nonhuman
priority journal
protein expression
M3 - Article
PY - 2011
SP - 146-153
ST - Biocompatibility studies on polyaniline and polyaniline-silver nanoparticle
coated polyurethane composite
TI - Biocompatibility studies on polyaniline and polyaniline-silver nanoparticle
coated polyurethane composite
79955871374&doi=10.1016%2fj.colsurfb.2011.03.033&partnerID=40&md5=946c5405e14cb1412
32182c7f20a26d5
VL - 86
ID - 5736
ER -
TY - JOUR
AB - Copper high aspect ratio structures (CuHARS) and silver cystine nanoparticles
(AgCysNPs) are two unique micro/nano particles under study here that show extensive
anti-cancer effects on a glioma tumor cell line. These micro/nano particles have
shown potent toxicity in the presence of inflammatory stimulus (combination of
tumor necrosis factor, [TNF] and lipo-polysaccharide, LPS). CuHARS with a
concentration of 20 μg/ml uniquely increased the catalytic generation of nitric
oxide (NO), an important contributor in the immune system. This NO was generated in
a cell culture tumor microenvironment (TME) in the presence of 25 µM S-nitrosothiol
(cysteine-NO) and the inflammatory stimulus. CuHARS increased the NO production by
68.75% when compared to untreated glioma cells with CysNO and inflammatory
stimulus. The production of NO was significantly higher under similar circumstances
in the case of normal primary structural cells like brain microvascular endothelial
cells (BMVECs). The production of NO by BMVECs went up by 181.25% compared to
glioma cells. This significant increase in the NO concentration could have added up
to tumorigenesis but the anti-cancer effect of CuHARS was prominent enough to lower
down the viability of glioma cells by approximately 20% and increased the
metabolism of structural cells, BMVECs by approximately 200%. The immunomodulatory
effect of NO in the TME under these circumstances in the presence of the novel
micro/nano material, CuHARS has risen up compared to the effect of inflammatory
stimulus alone. The potency and specific nature of these materials toward tumor
cells may make them suitable candidates for cancer treatment. Successive treatment
of CuHARS to glioma cells also proved to be an effective approach considering the
decrease in the total count of cells by 11.84 fold in case of three successive
treatments compared to a single dose which only decreased the cell count by 2.45
fold showing the dose-dependent increasing toxicity toward glioma cells. AgCysNPs
are another potent nanomaterial which also proved its significant toxic nature
toward tumor cell lines as demonstrated here, but their immunomodulatory response
is still unclear and needs to be explored further. © Copyright © 2021 Prajapati,
Karan, Khezerlou and DeCoster.
AU - Prajapati, N.
AU - Karan, A.
AU - Khezerlou, E.
AU - DeCoster, M. A.
C7 - 629835
DB - Scopus
DO - 10.3389/fchem.2020.629835
KW - brain microvascular endothelial cells
Copper/silver nano particles
glioma
inflammatory stimulus
nitric oxide
M3 - Article
PY - 2021
ST - The Immunomodulatory Potential of Copper and Silver Based Self-Assembled
Metal Organic Biohybrids Nanomaterials in Cancer Theranostics
TI - The Immunomodulatory Potential of Copper and Silver Based Self-Assembled
Metal Organic Biohybrids Nanomaterials in Cancer Theranostics
85100990317&doi=10.3389%2ffchem.2020.629835&partnerID=40&md5=85ef8271c7abd2e00fa1f4
a88ac11e1a
VL - 8
ID - 5205
ER -
TY - JOUR
AB - The biosynthesis of silver nanoparticles using plant extracts is a safe and
efficient method. Human periodontal stem cells (hPDLSCs) can promote periodontal
tissue formation for regenerative therapy. The current study aimed to biosynthesize
silver nanoparticles (AgNPs) using stem bark extracts from Oroxylum indicum (L)
Kurz (OI) as a reducing agent (OI/AgNPs) and investigate the biological properties
of OI/AgNPs on human periodontal ligament stem cells (hPDLSCs). The OI/AgNPs were
biosynthesized and characterized using UV–vis spectrophotometry (UV‒vis), Fourier
transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission
electron microscopy (TEM) and zeta potential analyses. The hPDLSCs were stimulated
with hydrogen peroxide (H2O2, H2O2-hPDLSCs) and bacterial lipopolysaccharide (LPS,
LPS-hPDLSCs) and encapsulated in the hydrogel. After that, the levels of cell
viability, interleukin-1beta (IL-1b), alkaline phosphatase (ALP) activity, and
calcium content were measured and compared with quercetin (QT) as a reference
flavonoid. The OI/AgNPs were stable spherical nanoparticles with a size range of
21.49 ± 0.32 nm, and biosynthesis enhanced the antioxidant and biological effects
of the OI/AgNPs. The accumulation of OI/AgNPs in the cytoplasm of hPDLSCs with high
levels of cell viability was demonstrated. OI/AgNPs increased the cell growth of
H2O2-hPDLSCs and decreased the levels of IL-1b secretion from LPS-hPDLSCs. The
OI/AgNPs increased the ALP activity and calcium content of the hPDLSCs. In
conclusion, the biosynthesized OI/AgNPs were noncytotoxic and could protect hPDLSCs
against oxidative stress and inflammatory stimuli and promote osteoblastic
differentiation; thus, they are applicable for the regenerative treatment of
peri-implantitis. © 2022 The Author(s)
AU - Prapaipittayakhun, J.
AU - Boonyuen, S.
AU - Zheng, A. L. T.
AU - Apinyauppatham, K.
AU - Arpornmaeklong, P.
C7 - 100117
DB - Scopus
DO - 10.1016/j.onano.2022.100117
KW - Anti-inflammatory agent
Cell differentiation
Cell encapsulation
Flavonoids
Multifunctional nanoparticle
Somatic adult stem cells
biological product
calcium
flavonoid
hydrogen peroxide
interleukin 1beta
Oroxylum indicum extract
plant extract
quercetin
silver nanoparticle
unclassified drug
Article
bioassay
biological activity
bone characteristics and functions
cell encapsulation
cell growth
cell isolation
cell viability
controlled study
cytotoxicity
encapsulation
flow cytometry
human
human cell
IC50
Oroxylum
osteogenic differentiation
oxidative stress
particle size
periodontal ligament stem cell
physical chemistry
protein expression
stem cell expansion
total flavonoid content assay
X ray diffraction
zeta potential
M3 - Article
PY - 2023
ST - Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on
human periodontal ligament stem cells
T2 - OpenNano
TI - Biologic effects of biosynthesized Oroxylum indicum/silver nanoparticles on
human periodontal ligament stem cells
85144519025&doi=10.1016%2fj.onano.2022.100117&partnerID=40&md5=c1e3597a38c4a24b0f28
b17ee2797848
VL - 9
ID - 4997
ER -
TY - JOUR
AB - Silver nanoparticles (Ag NP) have been shown to generate reactive oxygen
species; however, the association between physicochemical characteristics of
nanoparticles and cellular stress responses elicited by exposure has not been
elucidated. Here, we examined three key stress-responsive pathways activated by
Nrf-2/ARE, NFκB, and AP1 during exposure to Ag NP of two distinct sizes (10 and
75nm) and coatings (citrate and polyvinylpyrrolidone), as well as silver nitrate
(AgNO3), and CeO2 nanoparticles. The in vitro assays assessed the cellular response
in a battery of stable luciferase-reporter HepG2 cell lines. We further assessed
the impact of Ag NP and AgNO3 exposure on cellular redox status by measuring
glutathione depletion. Lastly, we determined intracellular Ag concentration by
inductively coupled plasma mass spectroscopy (ICP-MS) and re-analyzed reporter-gene
data using these values to estimate the relative potencies of the Ag NPs and AgNO3.
Our results show activation of all three stress response pathways, with Nrf-2/ARE
displaying the strongest response elicited by each Ag NP and AgNO3 evaluated here.
The smaller (10-nm) Ag NPs were more potent than the larger (75-nm) Ag NPs in each
stress-response pathway, and citrate-coated Ag NPs had higher intracellular silver
concentrations compared with both PVP-coated Ag NP and AgNO3. The cellular stress
response profiles after Ag NP exposure were similar to that of AgNO3, suggesting
that the oxidative stress and inflammatory effects of Ag NP are likely due to the
cytotoxicity of silver ions. © 2013 .
AU - Prasad, R. Y.
AU - McGee, J. K.
AU - Killius, M. G.
AU - Suarez, D. A.
AU - Blackman, C. F.
AU - DeMarini, D. M.
AU - Simmons, S. O.
DB - Scopus
DO - 10.1016/j.tiv.2013.07.005
IS - 6
KW - Nanoparticles
NRF2
Oxidative stress
Reporter genes
Silver
Stress response
Cell Survival
Cerium
Citric Acid
Genes, Reporter
Glutathione
Hep G2 Cells
Humans
Luciferases
Metal Nanoparticles
NF-kappa B
Oxidative Stress
Particle Size
Povidone
Replication Protein C
Silver Nitrate
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
50% inhibitory concentration
activator protein 1
Ag NPs
Ag(+)
AgNO(3)
antioxidant response element
AP1
ARE
BSO
CeO(2)
cerium oxide
DLS
dynamic light scattering
EC(50)
EC(max)
FBS
fetal bovine serum
glutathione
GSH
half maximal effective concentration
HQ
hydroquinone
IC(50)
ICP-MS
IL-1b
inductively coupled plasma mass spectroscopy
interleukin 1b
l-buthionine (S,R)-sulfoximine
maximal effective concentration
MTT
NFκB
nuclear factor (erythroid-derived 2)-like 2
nuclear factor kappa B
o-phenylenediamine
OPD
PdI
polydispersity index
polyvinylpyrrolidone
PVP
ROS
silver ion
silver nitrate
citric acid
lentivirus vector
povidone
silver nanoparticle
article
cell strain HepG2
controlled study
cytotoxicity
exposure
human
human cell
in vitro study
inflammation
light scattering
mass spectrometry
oxidative stress
particle size
reporter gene
M3 - Article
PY - 2013
SP - 2013-2021
ST - Investigating oxidative stress and inflammatory responses elicited by silver
nanoparticles using high-throughput reporter genes in HepG2 cells: Effect of size,
surface coating, and intracellular uptake
TI - Investigating oxidative stress and inflammatory responses elicited by silver
nanoparticles using high-throughput reporter genes in HepG2 cells: Effect of size,
surface coating, and intracellular uptake
84881249797&doi=10.1016%2fj.tiv.2013.07.005&partnerID=40&md5=c6cc2df24fecce00c03159
bb2275eaff
VL - 27
ID - 5699
ER -
TY - JOUR
AB - We showed previously that exposure of human lung cells (BEAS-2B) to TiO2
nanoparticles (nano-TiO2) produced micronuclei (MN) only when the final
concentration of protein in the cell-culture medium was at least 1%. Nanoparticles
localize in the liver; thus, we exposed human liver cells (HepG2) to nano-TiO2 and
found the same requirement for MN induction. Nano-TiO2 also formed small
agglomerates in medium containing as little as 1% protein and caused cellular
interaction as measured by side scatter by flow cytometry and DNA damage (comet
assay) in HepG2 cells. Nano-TiO2 also increased the activity of the inflammatory
factor NFkB but not of AP1 in a reporter-gene HepG2 cell line. Suspension of nano-
TiO2 in medium containing 0.1% protein was sufficient for induction of MN by the
nanoparticles in either BEAS-2B or HepG2 cells as long the final concentration of
protein in the cell-culture medium was at least 1%. Environ. Mol. Mutagen. 55:336-
342, 2014. (c) 2014 Wiley Periodicals, Inc.
AN - WOS:000333745800004
AU - Prasad, R. Y.
AU - Simmons, S. O.
AU - Killius, M. G.
AU - Zucker, R. M.
AU - Kligerman, A. D.
AU - Blackman, C. F.
AU - Fry, R. C.
AU - DeMarini, D. M.
DA - MAY
DO - 10.1002/em.21848
IS - 4
PY - 2014
SN - 0893-6692
1098-2280
SP - 336-342
ST - Cellular Interactions and Biological Responses to Titanium Dioxide
Nanoparticles in HepG2 and BEAS-2B Cells: Role of Cell Culture Media
T2 - ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
TI - Cellular Interactions and Biological Responses to Titanium Dioxide
Nanoparticles in HepG2 and BEAS-2B Cells: Role of Cell Culture Media
VL - 55
ID - 6636
ER -
TY - JOUR
AB - Injectable, drug-releasing hydrogel scaffolds with multifunctional properties
including hemostasis and anti-bacterial activity are essential for successful wound
healing; however, designing ideal materials is still challenging. Herein, we
demonstrate the fabrication of a biodegradable, temperature-pH dual responsive
supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl
caprolactam) (AG/PVCL) through free radical polymerization and the subsequent
chemical and ionic cross-linking. A natural therapeutic molecule, tannic acid (TA)-
incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound
healing efficiency were studied. In the AG/PVCL-TA system, TA acts as a therapeutic
molecule and also substitutes as an effective gelation binder. Notably, the
polyphenol-arm structure and diverse bonding abilities of TA can hold polymer
chains through multiple bonding and co-ordinate cross-linking, which were vital in
the formation of the mechanically robust AG/PVCL-TA. The SHG formation was
successfully balanced by varying the composition of SA, VCL, TA and cross-linkers.
The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a
sustained manner under physiological temperature-pH conditions. AG/PVCL-TA
displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and
cell proliferation activity towards the 3T3 fibroblast cell line. The wound healing
performance of AG/PVCL-TA was further confirmed in skin excision wound models,
which demonstrated the potential application of AG/PVCL-TA for skin regeneration
and rapid wound healing.
AN - WOS:000574915600007
AU - Preman, N. K.
AU - Priya, S. E. S.
AU - Prabhu, A.
AU - Shaikh, S. B.
AU - Vipin, C.
AU - Barki, R. R.
AU - Bhandary, Y. P.
AU - Rekha, P. D.
AU - Johnson, R. P.
DA - OCT 7
DO - 10.1039/d0tb01468k
IS - 37
PY - 2020
SN - 2050-750X
2050-7518
SP - 8585-8598
ST - Bioresponsive supramolecular hydrogels for hemostasis, infection control and
accelerated dermal wound healing
TI - Bioresponsive supramolecular hydrogels for hemostasis, infection control and
accelerated dermal wound healing
VL - 8
ID - 6711
ER -
TY - JOUR
AB - This study aimed to investigate the antioxidant, anti-inflammatory and
biosorption properties of starch nanocrystals (SNC). The characterization of
synthesized SNC was done using various analytical techniques like microscopic and
spectroscopic analysis. The antioxidant property was determined using DPPH (2,2-
diphenyl-1-picrylhydrazyl) assay and metal ion chelating assay. SNC showed the
highest scavenging activity of 70.03 +/- 0.74% at 100 mu g/mL concentration.
Protein denaturation assay and proteinase inhibitory assay depicted the anti-
inflammatory property of SNC. The results revealed that the maximum inhibition
activity was found at 100 mu g/mL with 72.71% inhibition. The maximum removal
efficiency was found to be 83.42% at pH 2.0 with 0.15 g biosorbent. As the pH
increases, biosorption capacity of SNC were reduced from 8.17 to 6.30 mg/g and the
efficiency of the dye removal was decreased from 80.95 to 36.01%. The shape of
synthesized SNC was spherical nanoplatelets and it shows agglomeration. The
Langmuir isotherm model is best suited for the biosorption experiments with the R2
value of 0.986. SNC were subjected to cytotoxic and phytotoxic evaluation. Cell
viability and phytotoxic assays proves the non-toxic nature of the SNC.
AN - WOS:000578649600001
AU - Priyan, V. V.
AU - Shahnaz, T.
AU - Kunnumakkara, A. B.
AU - Rana, V.
AU - Saravanan, M.
AU - Narayanasamy, S.
C6 - OCT 2020
DA - SEP
DO - 10.1007/s10876-020-01905-5
IS - 5
PY - 2021
SN - 1040-7278
1572-8862
SP - 1419-1430
ST - Antioxidant, Anti-inflammatory and Biosorption Properties of Starch
Nanocrystals In Vitro Study: Cytotoxic and Phytotoxic Evaluation
TI - Antioxidant, Anti-inflammatory and Biosorption Properties of Starch
Nanocrystals In Vitro Study: Cytotoxic and Phytotoxic Evaluation
VL - 32
ID - 6040
ER -
TY - JOUR
AB - ABSTRACT Objectives To describe acute and sub acute aspects of histological
and immunohistochemical response to PP implant in a rat subcutaneous model based on
objective methods. Materials and Methods Thirty rats had a PP mesh subcutaneously
implanted and the same dissection on the other side of abdomen but without mesh
(sham). The animals were euthanized after 4 and 30 days. Six slides were prepared
using the tissue removed: one stained with hematoxylin-eosin (inflammation
assessment); one unstained (birefringence evaluation) and four slides for
immunohistochemical processing: IL-1 and TNF-α (pro-inflammatory cytokines), MMP-2
(collagen metabolism) and CD-31 (angiogenesis). The area of inflammation, the
birefringence index, the area of immunoreactivity and the number of vessels were
objectively measured. Results A larger area of inflammatory reaction was observed
in PP compared to sham on the 4th and on the 30th day (p=0.0002). After 4 days, PP
presented higher TNF (p=0.0001) immunoreactivity than sham and no differences were
observed in MMP-2 (p=0.06) and IL-1 (p=0.08). After 30 days, a reduction of IL-1
(p=0.010) and TNF (p=0.016) for PP and of IL-1 (p=0.010) for sham were observed.
Moreover, area of MMP-2 immunoreactivity decreased over time for PP group
(p=0.018). Birefringence index and vessel counting showed no differences between PP
and sham (p=0.27 and p=0.58, respectively). Conclusions The implantation of
monofilament and macroporous polypropylene in the subcutaneous of rats resulted in
increased inflammatory activity and higher TNF production in the early post implant
phase. After 30 days, PP has similar cytokines immunoreactivity, vessel density and
extracellular matrix organization.
AD - Prudente, Alessandro
Universidade de Campinas. Faculdade de Ciências Médicas. Campinas. BR
Fávaro, Wágner José
Latuf Filho, Paulo
Riccetto, Cássio Luis Zanettini
AU - Prudente, Alessandro
AU - Fávaro, Wágner José
AU - Latuf Filho, Paulo
AU - Riccetto, Cássio Luis Zanettini
C1 - 20160630
DA - 2016/06
DB - LILACS
DO - 10.1590/S1677-5538.IBJU.2015.0289
IS - 3
KW - Foreign body reaction
Graft versus host reaction
Pelvic organ prolapse
Polypropylenes
Urinary incontinence
LA - en
PY - 2016
SN - 1677-5538
SP - 585-593
ST - Host inflammatory response to polypropylene implants: insights from a
quantitative immunohistochemical and birefringence analysis in a rat subcutaneous
model
T2 - Int. braz. j. urol
TI - Host inflammatory response to polypropylene implants: insights from a
quantitative immunohistochemical and birefringence analysis in a rat subcutaneous
model
55382016000300585
VL - 42
ID - 4931
ER -
TY - JOUR
AB - Mercury (Hg) is a global environmental contaminant, and excessive mercury
levels in water can adversely affect the growth of fish. Silver carp
(Hypophthalmichthys molitrix) is one of the important freshwater aquaculture fish
in China, and its natural resources have been critically declining. However, the
effects of Hg2+ exposure on the growth hormone/insulin-like growth factor (GH/IGF)
axis and its toxic mechanism are still unclear. In this study, we systematically
evaluated the bioaccumulation, histomorphology, antioxidant status, hormone levels,
and GH/ IGF axis toxicity of juvenile silver carp after exposure to environmental-
related concentrations of Hg2+ (0, 0.05, 0.5, 5, and 50 mu g/L) for 28 days.
Results showed that the Hg2+ bioaccumulation in the liver increased with a rise in
Hg2+ concentration and time of exposure. The body length (BL), body weight (BW),
weight growth rate (WGR) and specific growth rate (SGR) all decreased after Hg2+
exposure. The serum levels of growth hormones (GH and IGF) and thyroid hormones (T3
and T4) were significantly decreased, and the expressions of GH/IGF axis-related
genes were significantly downregulated after 7, 14, and 28 days of Hg2+ exposure.
Correlations between the growth parameters and growth hormones or expression of
genes in GH/IGF axis further suggested that environmentally relevant concentrations
of Hg2+ could have adverse effects on growth. In addition, with increasing Hg2+
exposure, superoxide activities of dismutase (SOD), catalase (CAT), and glutathione
S-transferase (GST)and levels of reduced glutathione (GSH) and malondialdehyde
(MDA) were significantly increased, whereas the activity of glutathione peroxidase
(GPx) significantly decreased and oxidative stress-related gene significantly
changed. Liver lesions were mainly characterized by inflammatory cell infiltration,
hepatocyte necrosis and fat vacuolation after exposure to Hg2+. Taken together, the
results indicate that Hg2+ exposure leads to growth inhibition and oxidative stress
in juvenile silver.
AN - WOS:000799000900007
AU - Pu, Y.
AU - Guo, J.
AU - Yang, H.
AU - Zhong, L. Q.
AU - Tian, H. W.
AU - Deng, H. T.
AU - Duan, X. B.
AU - Liu, S. P.
AU - Chen, D. Q.
C6 - APR 2022
C7 - 113484
DA - MAY 1
DO - 10.1016/j.ecoenv.2022.113484
PY - 2022
SN - 0147-6513
1090-2414
ST - Environmentally relevant concentrations of mercury inhibit the growth of
juvenile silver carp (Hypophthalmichthys molitrix): Oxidative stress and GH/IGF
axis
T2 - ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
TI - Environmentally relevant concentrations of mercury inhibit the growth of
axis
VL - 236
ID - 6578
ER -
TY - JOUR
AB - Mercury (Hg) is a global environmental contaminant, and excessive mercury
levels in water can adversely affect the growth of fish. Silver carp
(Hypophthalmichthys molitrix) is one of the important freshwater aquaculture fish
in China, and its natural resources have been critically declining. However, the
effects of Hg2+ exposure on the growth hormone/insulin-like growth factor (GH/IGF)
axis and its toxic mechanism are still unclear. In this study, we systematically
evaluated the bioaccumulation, histomorphology, antioxidant status, hormone levels,
and GH/IGF axis toxicity of juvenile silver carp after exposure to environmental-
related concentrations of Hg2+ (0, 0.05, 0.5, 5, and 50 µg/L) for 28 days. Results
showed that the Hg2+ bioaccumulation in the liver increased with a rise in Hg2+
concentration and time of exposure. The body length (BL), body weight (BW), weight
growth rate (WGR) and specific growth rate (SGR) all decreased after Hg2+ exposure.
The serum levels of growth hormones (GH and IGF) and thyroid hormones (T3 and T4)
were significantly decreased, and the expressions of GH/IGF axis-related genes were
significantly downregulated after 7, 14, and 28 days of Hg2+ exposure. Correlations
between the growth parameters and growth hormones or expression of genes in GH/IGF
axis further suggested that environmentally relevant concentrations of Hg2+ could
have adverse effects on growth. In addition, with increasing Hg2+ exposure,
superoxide activities of dismutase (SOD), catalase (CAT), and glutathione S-
transferase (GST)and levels of reduced glutathione (GSH) and malondialdehyde (MDA)
were significantly increased, whereas the activity of glutathione peroxidase (GPx)
significantly decreased and oxidative stress-related gene significantly changed.
Liver lesions were mainly characterized by inflammatory cell infiltration,
hepatocyte necrosis and fat vacuolation after exposure to Hg2+. Taken together, the
results indicate that Hg2+ exposure leads to growth inhibition and oxidative stress
in juvenile silver. © 2022 The Authors
AU - Pu, Y.
AU - Guo, J.
AU - Yang, H.
AU - Zhong, L.
AU - Tian, H.
AU - Deng, H.
AU - Duan, X.
AU - Liu, S.
AU - Chen, D.
C7 - 113484
DB - Scopus
DO - 10.1016/j.ecoenv.2022.113484
KW - Growth
Growth hormone/insulin-like growth factor (GH/IGF) axis
Mercury
Oxidative stressHypophthalmichthys molitrix
Animals
Carps
Growth Hormone
Oxidative Stress
Somatomedins
catalase
glutathione
growth hormone
malonaldehyde
mercury
somatomedin
antioxidant
cyprinid
growth response
hormone
juvenile
oxidative stress
pollution exposure
animal cell
animal experiment
animal tissue
aquatic environment
Article
bioaccumulation
body height
body weight
body weight gain
cell infiltration
cell vacuole
controlled study
gene expression
growth inhibition
growth rate
histopathology
juvenile animal
nonhuman
protein expression
thyroid hormone blood level
toxicity testing
water pollution
animal
carp
genetics
metabolism
M3 - Article
PY - 2022
ST - Environmentally relevant concentrations of mercury inhibit the growth of
axis
TI - Environmentally relevant concentrations of mercury inhibit the growth of
axis
85128192238&doi=10.1016%2fj.ecoenv.2022.113484&partnerID=40&md5=44eaec3c3a8acdaf84b
d395c29f4af71
VL - 236
ID - 5065
ER -
TY - JOUR
AB - Current commercially available silver-based wound dressings such as silver-
nylon have been used as antimicrobial barriers for burn and trauma care in combat
conditions for over 10 years. However, these dressings do not stabilize the eschar
or reduce its toxicity. Cerium nitrate (CN) solutions have been established
clinically to stabilize the eschar by decreasing release of inflammatory mediators
from burned tissue thereby allowing delayed excision and grafting. In this report,
we tested the extent to which CN imparts CN benefits to silver dressings for
temporizing treatments of burn wounds and enhancing anti-bacterial activity. Using
a rat full-thickness scald burn model, we showed that CN enhanced the anti-
bacterial effects of the tested silver-based dressings (Acticoat (TM), Mepilex (TM)
and Silverlon (R)), while also imparting anti-inflammatory properties to these
dressings. Compared to the use of silver dressings alone, CN significantly
decreased the levels of IL-1 beta and GRO/KC, and exhibited downward trending
levels of IL-1 alpha, MIP-1 alpha, and bacterial bioburden within the wound. Based
on our findings, we conclude that CN has the ability to expand and enhance the
function of several silver dressings. We propose the use of CN in combination with
silver dressings to stabilize burn wounds thereby allowing postponement of excision
and grafting, most notably in scenarios where the standard of care is not feasible
such as in combat situations, resource limited regions, and new emergent health
care challenges as seen during the COVID-19 pandemic in which COVID-positive severe
burn patients are not able to undergo surgery during an active outbreak.
AN - WOS:000577526700001
AU - Qian, L. W.
AU - Fourcaudot, A. B.
AU - Chen, P.
AU - Brandenburg, K. S.
AU - Weaver, A. J.
AU - Leung, K. P.
IS - 4
PY - 2020
SN - 2160-2026
SP - 91-100
ST - Cerium nitrate enhances anti-bacterial effects and imparts anti-inflammatory
properties to silver dressings in a rat scald burn model
T2 - INTERNATIONAL JOURNAL OF BURNS AND TRAUMA
TI - Cerium nitrate enhances anti-bacterial effects and imparts anti-inflammatory
properties to silver dressings in a rat scald burn model
VL - 10
ID - 5956
ER -
TY - JOUR
AB - Dental implants with proper antibacterial ability as well as ideal
osseointegration are being actively pursued. The antimicrobial ability of titanium
implants can be significantly enhanced via modification with silver nanoparticles
(Ag NPs). However, the high mobility of Ag NPs results in their potential
cytotoxicity. The silver plasma immersion ion-implantation (Ag-PIII) technique may
remedy the defect. Accordingly, Ag-PIII technique was employed in this study in an
attempt to reduce the mobility of Ag NPs and enhance osseointegration of
sandblasted and acid-etched (SLA) dental implants. Briefly, 48 dental implants,
divided equally into one control and three test groups (further treated by Ag-PIII
technique with three different implantation parameters), were inserted in the
mandibles of six Labrador dogs. Scanning electron microscopy, X-ray photoelectron
spectroscopy, and inductively coupled plasma optical emission spectrometry were
used to investigate the surface topography, chemical states, and silver release of
SLA- and Ag-PIII-treated titanium dental implants. The implant stability quotient
examination, Microcomputed tomography evaluation, histological observations, and
histomorphometric analysis were performed to assess the osseointegration effect in
vivo. The results demonstrated that normal soft tissue healing around dental
implants was observed in all the groups, whereas the implant stability quotient
values in Ag-PIII groups were higher than that in the SLA group. In addition, all
the Ag-PIII groups, compared to the SLA-group, exhibited enhanced new bone
formation, bone mineral density, and trabecular pattern. With regard to osteogenic
indicators, the implants treated with Ag-PIII for 30 minutes and 60 minutes, with
the diameter of the Ag NPs ranging from 5-25 nm, were better than those treated
with Ag-PIII for 90 minutes, with the Ag NPs diameter out of that range. These
results suggest that Ag-PIII technique can reduce the mobility of Ag NPs and
enhance the osseointegration of SLA surfaces and have the potential for future use.
AN - WOS:000347783900001
AU - Qiao, S. C.
AU - Cao, H. L.
AU - Zhao, X.
AU - Lo, H. W.
AU - Zhuang, L. F.
AU - Gu, Y. X.
AU - Shi, J. Y.
AU - Liu, X. Y.
AU - Lai, H.
DO - 10.2147/IJN.S73467
PY - 2015
SN - 1178-2013
SP - 653-664
ST - Ag-plasma modification enhances bone apposition around titanium dental
implants: an animal study in Labrador dogs
TI - Ag-plasma modification enhances bone apposition around titanium dental
implants: an animal study in Labrador dogs
VL - 10
ID - 6399
ER -
TY - JOUR
AB - Inflammatory macrophages play pivotal roles in the development of
atherosclerosis. Theranostics, a promising approach for local imaging and
photothermal therapy of inflammatory macrophages, has drawn increasing attention in
biomedical research. In this study, gold nanorods (Au NRs) were synthesized, and
their in vitro photothermal effects on the macrophage cell line (Ana-1 cells) under
808 nm near infrared reflection (NIR) were investigated by the CCK8 assay, calcein
AM/PI staining, flow cytometry, transmission electron microscopy (TEM), silver
staining and in vitro micro-computed tomography (CT) imaging. These Au NRs were
then applied to an apolipoprotein E knockout (Apo E) mouse model to evaluate their
effects on in vivo CT imaging and their effectiveness as for the subsequent
photothermal therapy of macrophages in femoral artery restenosis under 808 nm laser
irradiation. In vitro photothermal ablation treatment using Au NRs exhibited a
significant cell-killing efficacy of macrophages, even at relatively low
concentrations of Au NRs and low NIR powers. In addition, the in vivo results
demonstrated that the Au NRs are effective for in vivo imaging and photothermal
therapy of inflammatory macrophages in femoral artery restenosis. This study shows
that Au nanorods are a promising theranostic platform for the diagnosis and
photothermal therapy of inflammation-associated diseases.
AN - WOS:000359546900023
AU - Qin, J. B.
AU - Peng, Z. Y.
AU - Li, B.
AU - Ye, K. C.
AU - Zhang, Y. X.
AU - Yuan, F. K.
AU - Yang, X. R.
AU - Huang, L. J.
AU - Hu, J. Q.
AU - Lu, X. W.
DO - 10.1039/c5nr02521d
IS - 33
PY - 2015
SN - 2040-3364
2040-3372
SP - 13991-14001
ST - Gold nanorods as a theranostic platform for in vitro and in vivo imaging and
photothermal therapy of inflammatory macrophages
T2 - NANOSCALE
TI - Gold nanorods as a theranostic platform for in vitro and in vivo imaging and
photothermal therapy of inflammatory macrophages
VL - 7
ID - 6568
ER -
TY - JOUR
AB - Diabetic wounds are one of the most challenging public health issues of the
21st century due to their inadequate vascular supply, bacterial infections, high
levels of oxidative stress, and abnormalities in antioxidant defenses, whereas
there is no effective treatment for diabetic wounds. Due to the distinct properties
of nanoparticles, such as their small particle size, elevated cellular uptake, low
cytotoxicity, antibacterial activity, good biocompatibility, and biodegradability.
The application of nanoparticles has been widely used in the treatment of diabetic
wound healing due to their superior anti-inflammatory, antibacterial, and
antioxidant activities. These nanoparticles can also be loaded with various agents,
such as organic molecules (eg, exosomes, small molecule compounds, etc.), inorganic
molecules (metals, nonmetals, etc.), or complexed with various biomaterials, such
as smart hydrogels (HG), chitosan (CS), and hyaluronic acid (HA), to augment their
therapeutic potential in diabetic wounds. This paper reviews the therapeutic
potential and future perspective of nanoparticles in the treatment of diabetic
wounds. Together, nanoparticles represent a promising strategy in the treatment of
diabetic wound healing. The future direction may be to develop novel nanoparticles
with multiple effects that not only act in wound healing at all stages of diabetes
but also provide a stable physiological environment throughout the wound-healing
process. © 2022 Qin et al.
AU - Qin, W.
AU - Wu, Y.
AU - Liu, J.
AU - Yuan, X.
AU - Gao, J.
DB - Scopus
DO - 10.2147/IJN.S386585
KW - biomaterials
diabetic complications
diabetic wound
nanoparticles
wound healing
Antioxidants
Chitosan
Diabetes Mellitus
Humans
Hydrogels
Nanoparticles
Wound Healing
botulinum toxin
Ceria Nanoparticle
chitosan
copper nanoparticle
Ganglioside monosialic acid 3
gelatinase A
gelatinase B
gold nanoparticle
heme oxygenase 1
heparin
hyaluronic acid
hydrogel
hypertensive factor
hypoxia inducible factor 1
interleukin 10
interleukin 12
interleukin 6
interleukin 8
methylcellulose
nanoparticle
nitric oxide
platelet derived growth factor B
polycaprolactone
polyethyleneimine
protein kinase B
pullulan
quercetin
Rubidium Nanoparticle
silica nanoparticle
silver nanoparticle
somatomedin C
stromal cell derived growth factor 1
transforming growth factor alpha
unclassified drug
vasculotropin
vasculotropin A
antiinfective agent
antioxidant
angiogenesis
bacterial infection
biocompatibility
biodegradability
cell migration
cell proliferation
cytokine production
cytokine release
cytotoxicity
diabetes mellitus
exosome
human
nonhuman
oxidative stress
protein expression
Review
signal transduction
vascular disease
M3 - Review
PY - 2022
SP - 6007-6029
ST - A Comprehensive Review of the Application of Nanoparticles in Diabetic Wound
Healing: Therapeutic Potential and Future Perspectives
TI - A Comprehensive Review of the Application of Nanoparticles in Diabetic Wound
Healing: Therapeutic Potential and Future Perspectives
85144584615&doi=10.2147%2fIJN.S386585&partnerID=40&md5=744e9631b75e443643dd450627ee
a2d4
VL - 17
ID - 5152
ER -
TY - JOUR
AB - Xenogeneic scaffolds derived from the decellularized pancreas are plausible
biomedical materials for pancreatic tissue engineering applications. During the
decellularized process, the ultrastructure of extracellular matrices, including
collagen fibers, was destructed, which leads to the decrease of mechanical strength
and the immune-inflammatory response after transplantation in vivo. The cross-
linking method plays an important role in increasing mechanical strength and
reducing the inflammatory potential of decellularized scaffolds. However, no ideal
cross-linking agent has been identified for decellularized pancreatic scaffolds
yet. In this study, a cyclic perfusion system was used to cross-link decellularized
pancreatic scaffolds from Sprague Dawley rat with silver nanoparticles (AgNPs). The
optimum concentration of AgNPs was selected according to the scanning electron
microscope observation and mechanical evaluation, as well as cytotoxicity to human
umbilical vein endothelial cells and MIN-6 cell lines in vitro. The inflammation
after transplantation in vivo was evaluated by hematoxylin and eosin staining;
M1/M2 polarization phenotype of macrophages was further evaluated. Our results
showed that after cross-linking, the scaffold possessed better mechanical property
and biocompatibility, with the polarization of M2 macrophages increased. Thus,
AgNP-cross-linked pancreatic acellular scaffold can provide an ideal scaffold
source for pancreatic tissue engineering.
AN - WOS:000760270600005
AU - Qiu, H. Q.
AU - Zhang, L.
AU - Wang, D. Z.
AU - Miao, H. Y.
DA - FEB 1
DO - 10.1089/cell.2021.0071
IS - 1
PY - 2022
SN - 2152-4971
2152-4998
SP - 38-47
ST - Silver Nanoparticles Improve the Biocompatibility and Reduce the
Immunogenicity of Xenogeneic Scaffolds Derived from Decellularized Pancreas
T2 - CELLULAR REPROGRAMMING
TI - Silver Nanoparticles Improve the Biocompatibility and Reduce the
VL - 24
ID - 5875
ER -
TY - JOUR
AB - Xenogeneic scaffolds derived from the decellularized pancreas are plausible
biomedical materials for pancreatic tissue engineering applications. During the
decellularized process, the ultrastructure of extracellular matrices, including
collagen fibers, was destructed, which leads to the decrease of mechanical strength
and the immune-inflammatory response after transplantation in vivo. The cross-
linking method plays an important role in increasing mechanical strength and
reducing the inflammatory potential of decellularized scaffolds. However, no ideal
cross-linking agent has been identified for decellularized pancreatic scaffolds
yet. In this study, a cyclic perfusion system was used to cross-link decellularized
pancreatic scaffolds from Sprague Dawley rat with silver nanoparticles (AgNPs). The
optimum concentration of AgNPs was selected according to the scanning electron
microscope observation and mechanical evaluation, as well as cytotoxicity to human
umbilical vein endothelial cells and MIN-6 cell lines in vitro. The inflammation
after transplantation in vivo was evaluated by hematoxylin and eosin staining;
M1/M2 polarization phenotype of macrophages was further evaluated. Our results
showed that after cross-linking, the scaffold possessed better mechanical property
and biocompatibility, with the polarization of M2 macrophages increased. Thus,
AgNP-cross-linked pancreatic acellular scaffold can provide an ideal scaffold
source for pancreatic tissue engineering. © 2022 Mary Ann Liebert Inc.
AU - Qiu, H.
AU - Zhang, L.
AU - Wang, D.
AU - Miao, H.
DB - Scopus
DO - 10.1089/cell.2021.0071
IS - 1
KW - cross-linking
decellularized
macrophage polarization
pancreas
Animals
Endothelial Cells
Metal Nanoparticles
Pancreas
Rats
Silver
Tissue Engineering
Tissue Scaffolds
eosin
hematoxylin
silver nanoparticle
metal nanoparticle
silver
animal cell
animal experiment
animal model
animal tissue
Article
biocompatibility
cell transplantation
cell ultrastructure
cell viability
controlled study
cross linking
cytotoxicity
cytotoxicity test
decellularization
decellularized extracellular matrix
degradation
histopathology
immunity
immunogenicity
in vitro study
in vivo study
inflammatory cell
M1 macrophage
M2 macrophage
MIN6 cell line
nonhuman
pancreas tissue
perfusion
phenotype
polarization
rat
Sprague Dawley rat
tensile strength
tissue transplantation
xenograft
animal
chemistry
endothelium cell
procedures
tissue engineering
tissue scaffold
M3 - Article
PY - 2022
SP - 38-47
ST - Silver Nanoparticles Improve the Biocompatibility and Reduce the
T2 - Cellular Reprogramming
TI - Silver Nanoparticles Improve the Biocompatibility and Reduce the
85125001870&doi=10.1089%2fcell.2021.0071&partnerID=40&md5=6632bb1de52a5c2d515f7d139
ecc5ed0
VL - 24
ID - 5098
ER -
TY - JOUR
AB - BACKGROUND: Epidemiological studies have shown that exposure to ambient fine
particulate matter with aerodynamic diameter less than or equal to 2.5 μm (PM2:5 )
correlates with a decrease in sperm count, but the biological mechanism remains
elusive. OBJECTIVES: This study tested whether hypothalamic inflammation, an
emerging pathophysiological mediator, mediates the development of lower epididymal
sperm count due to PM2:5 exposure. METHODS: Inhibitor jB kinase 2 (IKK2) was
conditionally knocked out either in all neurons or subtypes of hypothalamic neurons
of mice. Effects of concentrated ambient PM2:5 (CAP) exposure on hypothalamic
inflammation, the hypothalamic–pituitary–gonadal (HPG) axis, and epididymal sperm
count of these mouse models were then assessed. Furthermore, to test whether
hypothalamic inflammation is sufficient to decrease sperm production, we
overexpressed constitutively active IKK2 (IKK2ca) either in all neurons or subtypes
of hypothalamic neurons and assessed hypothalamic inflam-mation, the HPG axis, and
sperm production of these overexpression mouse models. RESULTS: CAP-exposed wild-
type control mice vs. filtered air (FA)-exposed wild-type control mice had a higher
expression of hypothalamic inflammatory markers, lower functional indexes of the
HPG axis, and a lower epididymal sperm count. In contrast, all these measurements
for CAP-vs. FA-exposed mice deficient of IKK2 in all neurons were comparable. We
also found that overexpression of IKK2ca in either all neurons or pro-
opiomelanocortin (POMC) neurons only, but not in Agouti-related protein (AgRP)
neurons only, resulted in lower functional indexes of the HPG axis and a lower
epididymal sperm count. Moreover, we showed that CAP-vs. FA-exposed mice deficient
of IKK2 in POMC neurons had a comparable expression of hypothalamic inflammatory
markers, comparable functional indexes of the HPG axis, and a comparable epididymal
sperm count. DISCUSSION: This mouse model study shows a causal role of IKK2 of POMC
neurons in the development of lower epididymal sperm count due to PM2:5 exposure,
providing a mechanistic insight into this emerging pathogenesis.
https://doi.org/10.1289/EHP8868. © 2021, Public Health Services, US Dept of Health
and Human Services. All rights reserved.
AU - Qiu, L.
AU - Chen, M.
AU - Wang, X.
AU - Chen, S.
AU - Ying, Z.
C7 - 097006
DB - Scopus
DO - 10.1289/EHP8868
IS - 9
KW - Animals
Hypothalamus
Male
Mice
Neurons
Particulate Matter
Pro-Opiomelanocortin
Spermatogenesis
3(or 17)beta hydroxysteroid dehydrogenase
agouti related protein
aluminum
androgen receptor
barium
bromine
cadmium
calcium
cerium
cholesterol monooxygenase (side chain cleaving)
chromium
copper
cre recombinase
cytochrome P450
cytochrome P450 family 17 alpha
erbium
estradiol
follitropin
gold
gonadorelin
I kappa B kinase beta
interleukin 1beta
interleukin 6
iridium
iron
kappa B Kinase 2 inhibitor
lead
luteinizing hormone
lutetium
magnesium
manganese
mercury
messenger RNA
molybdenum
nestin
nickel
pentobarbital
phosphorus
platinum
potassium
praseodymium
proopiomelanocortin
protein inhibitor
rubidium
selenium
sex hormone binding globulin
silicon
silver
sodium
steroidogenic acute regulatory protein
strontium
testosterone
testosterone 17beta dehydrogenase
thallium
tin
titanium
tungsten
unclassified drug
vanadium
zinc
zirconium
adult
aerodynamics
animal cell
animal experiment
animal model
animal tissue
antigen retrieval
Article
chemiluminescence immunoassay
controlled study
environmental factor
epidemiological monitoring
gene overexpression
germ cell
hypothalamic inflammation
hypothalamus hypophysis system
hypothalamus nucleus
male
mass spectrometry
mouse
nerve cell culture
nonhuman
particulate matter
protein expression
semen analysis
seminiferous tubule
Sertoli cell
sperm count
spermatocele
spermatogenesis
spermatogonium
testis weight
versatile aerosol concentration enrichment system
animal
hypothalamus
metabolism
nerve cell
toxicity
M3 - Article
PY - 2021
ST - Pm2:5 exposure of mice during spermatogenesis: A role of inhibitor jb kinase
2 in pro-opiomelanocortin neurons
T2 - Environmental Health Perspectives
TI - Pm2:5 exposure of mice during spermatogenesis: A role of inhibitor jb kinase
2 in pro-opiomelanocortin neurons
85115376790&doi=10.1289%2fEHP8868&partnerID=40&md5=112f04965cd7ce7b60b4bf0159e82aa4
VL - 129
ID - 5168
ER -
TY - GEN
AB - Os eventos histológicos no fígado foram quantificados, usando técnica de
imunomarcação para avaliar 53 amostras hepáticas provenientes de viscerotomia de
pacientes vitimados por febre amarela silvestre. A análise quantitativa dos eventos
demonstrou amplo predomínio do componente apoptótico sobre a necrose. O infiltrado
inflamatório é desproporcional em intensidade à morte dos hepatócitos. Houve
predomínio de linfócitos TCD4+, ocorrendo em menor proporção linfócitos TCD8+,
linfócitos B, células NK e apresentadoras de antígenos (S100). A expressão
citocínica foi de perfil Th1, com expressão de TNF-a, IFN-g e acompanhada de
intensa imunomarcação para TGF-b. Frente aos achados acreditamos que a predileção
pela localização médio zonal das lesões do fígado poderia decorrer de fenômenos de
hipóxia por hipofluxo secundária à vasculopatia sistêmica e associada ao efeito
citopático viral.In this work, the histological events in the liver were
quantified, using a immunomarking technique to evaluate 53 hepatic samples from the
viscerotomy of patients with the sylvatic form of yellow fever. Quantitative
analysis of the events demonstrated an ample prevalence of an apoptotic component
in the necrosis. The intensity of the inflammatory infiltration is disproportionate
to the death of the hepatocytes. There is a prevalence of TCD4+ lymphocytes, with a
smaller proportion of TCD8+ lymphocytes, B lymphocytes, NK cells and antigen-
presenting cells (S100). The cytokine expression presented a profile of Th1, with
expression of TNF-a, IFN-g and accompanied by intense immunomarking by TGF-b. Faced
with these findings, we consider that the predilection for the midzonal location of
the lesions in the liver could arise from the phenomena of hypoxia secondary to
systemic vasculopathy associated to the viral cytopathic effect...
AU - Quaresma, Juarez Antônio Simões
C1 - 20050729
C8 - lil-408984
DA - 2003/00
DB - LILACS
LA - pt
PY - 2003
SP - [162]-[162]
ST - Arboviroses da Amazônia: estudo do mecanismo de morte celular e da resposta
fenotípica do hospedeiro na febre amarela
TI - Arboviroses da Amazônia: estudo do mecanismo de morte celular e da resposta
fenotípica do hospedeiro na febre amarela
TT - Arboviruses of the Amazonia: study of the cell death mechanism of cell death
and the fenotipic host response in the yellow fever[UR -
https://pesquisa.bvsalud.org/portal/resource/pt/lil-408984
ID - 4956
ER -
TY - JOUR
AB - Tissue engineered vascular grafts (TEVGs) are beginning to achieve clinical
success and hold promise as a source of grafting material when donor grafts are
unsuitable or unavailable. Significant technological advances have generated small-
diameter TEVGs that are mechanically stable and promote functional remodeling by
regenerating host cells. However, developing a biocompatible blood-contacting
surface remains a major challenge. The TEVG luminal surface must avoid negative
inflammatory responses and thrombogenesis immediately upon implantation and promote
endothelialization. The surface has therefore become a primary focus for research
and development efforts. The current state of TEVGs is herein reviewed with an
emphasis on the blood-contacting surface. General vascular physiology and
developmental challenges and strategies are briefly described, followed by an
overview of the materials currently employed in TEVGs. The use of biodegradable
materials and stem cells requires careful control of graft composition, degradation
behavior, and cell recruitment ability to ensure that a physiologically relevant
vessel structure is ultimately achieved. The establishment of a stable monolayer of
endothelial cells and the quiescence of smooth muscle cells are critical to the
maintenance of patency. Several strategies to modify blood-contacting surfaces to
resist thrombosis and control cellular recruitment are reviewed, including coatings
of biomimetic peptides and heparin. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA,
Weinheim
AU - Radke, D.
AU - Jia, W.
AU - Sharma, D.
AU - Fena, K.
AU - Wang, G.
AU - Goldman, J.
AU - Zhao, F.
C7 - 1701461
DB - Scopus
DO - 10.1002/adhm.201701461
IS - 15
KW - blood-contacting surfaces
endothelialization
surface modification
vascular engineering
vascular grafts
Animals
Blood Vessel Prosthesis Implantation
Collagen
Humans
Swine
Tissue Engineering
Tissue Scaffolds
Biocompatibility
Biomimetics
Cytology
Endothelial cells
Grafts
Muscle
Stem cells
Surface treatment
Tissue engineering
argatroban
CD133 antigen
CD34 antibody
CD59 antigen
collagen
complement component C1s inhibitor
complement factor H
complement factor I
decay accelerating factor
elastin
heparin
membrane cofactor protein
nitric oxide
politef
polycaprolactone
polyethylene terephthalate
polyglactin
polyglycolic acid
polylactic acid
silk fibroin
silver nanoparticle
thrombin
thrombomodulin
tropoelastin
Biodegra-dable materials
Contacting surfaces
Endothelialization
Research and development
Technological advances
Tissue engineered vascular grafts
Vascular engineering
Vascular grafts
biocompatibility
biodegradation
blood clotting
blood flow
blood pressure
blood vessel tone
bone marrow derived mononuclear cell
cell activation
cell engineering
colony forming cell
dynamic cell seeding
electrostatic cell seeding
endothelial colony forming cell
endothelium cell
enzyme activation
graft rejection
hemostasis
human
in vitro study
inflammation
magnetic cell seeding
nonhuman
priority journal
protein secretion
Review
static cell seeding
thrombocyte
thrombosis
tissue engineering
animal
blood vessel prosthesis
blood vessel transplantation
chemistry
pig
procedures
tissue scaffold
Blood
M3 - Review
PY - 2018
ST - Tissue Engineering at the Blood-Contacting Surface: A Review of Challenges
and Strategies in Vascular Graft Development
TI - Tissue Engineering at the Blood-Contacting Surface: A Review of Challenges
and Strategies in Vascular Graft Development
85046403857&doi=10.1002%2fadhm.201701461&partnerID=40&md5=29eacdb603676b92c2cc1b4d7
9165dc9
VL - 7
ID - 5416
ER -
TY - JOUR
AB - Introduction: Silver nanomaterials; the engineered nanomaterials; have many
industrial applications. They are used in manufacture of cosmetic and daily used
products as food and clothes. Nanoparticles can pass through different biological
body barriers, accumulate in the female reproductive organs as uterus and ovaries
and exert their toxicity. Aim of the Work: To evaluate the effect of silver
nanoparticles on the histological structure of the endometrium using different
histological and immunohistochemical techniques. Materials and Methods: Thirty-six
adult female albino rats were divided into three equal groups; group I (control
group), group II and group III. Silver nanoparticles were administered daily for 2
weeks at dose 30mg /kg and 300mg/kg respectively orally to group II and III. The
size of the used nanoparticles is 20 nm. Specimens of uterus were taken to be
processed for examination by light microscope (haematoxylin and eosin, Masson
trichrome and TNF- alpha immunohistochemical staining) and transmission electron
microscope. Results: AgNps-treated animals showed stratification, cytoplasmic
vacuolation, discontinuation and desquamation in endometrial epithelial cells. The
lamina propria showed cellular infiltration (inflammatory reaction), empty spaces
and increase the deposition of collagen fibers (fibrosis). Ultrastructurally, they
showed focal loss of the apical microvilli of the endometrial epithelial cells,
apical cytoplasmic vacuoles, swollen, distorted and irregularly arranged
mitochondria and large amount of secondary lysosomes and autophagic vacuoles. Focal
separation of the epithelial cells with destruction of the intercellular junctions
was seen. The lamina propria showed accumulation of collagen fibres and
eosinophilic infiltration. Immunohistochemical study revealed highly significant
increase in the TNF- alpha immunoexpression particularly in group III. All these
changes were more severe in animals with high dose than those of low dose.
Conclusion: Silver nanoparticles induce structural changes in a dose dependent
manner in the endometrium. So, it should be given cautiously to females to avoid
uterine damage. EJH copyright © 2022. All rights served.
AU - Ragab, A. M. H.
AU - Ragab, M.
AU - Tawfik, S. M.
DB - Scopus
DO - 10.21608/ejh.2021.74346.1472
IS - 3
KW - Endometrium rat
proestrous phase
M3 - Article
PY - 2022
SP - 756-773
ST - Histological and Immunohistochemical Alterations Induced by Exposure to
Different Doses of Silver Nanoparticles in the Endometrium of Adult Albino Rat
TI - Histological and Immunohistochemical Alterations Induced by Exposure to
Different Doses of Silver Nanoparticles in the Endometrium of Adult Albino Rat
85144770662&doi=10.21608%2fejh.2021.74346.1472&partnerID=40&md5=ff8a9ee4a52b936fd11
3505d178547f8
VL - 45
ID - 5079
ER -
TY - JOUR
AB - Hydrogel-based drug delivery systems have emerged as a promising platform for
chronic tissue defects owing to their inherent ability to inhibit pathogenic
infection and accelerate rapid tissue regeneration. Here, we fabricated a stable
bio-hybrid hydrogel system comprising collagen, aminated xanthan gum, bio-capped
silver nanoparticles and melatonin with antimicrobial, antioxidant and anti-
inflammatory properties. Highly colloidal bio-capped silver nanoparticles were
synthesized using collagen as a reducing cum stabilizing agent for the first time
while aminated xanthan gum was synthesized using ethylenediamine treatment on
xanthan gum. The synthesized bio-hybrid hydrogel exhibits better gelation, surface
morphology, rheology and degelation properties. In vitro assessment of bio-hybrid
hydrogel demonstrates excellent bactericidal efficiency against both common wound
and multidrug-resistant pathogens and biocompatibility properties. In vivo animal
studies demonstrate rapid tissue regeneration, collagen deposition and angiogenesis
at the wound site predominantly due to the synergistic effect of silver
nanoparticles and melatonin in the hydrogel. This study paves the way for
developing biologically functional bio-nano hydrogel systems for promoting
effective care for various ailments, including infected chronic wounds. © 2021
Elsevier B.V.
AU - Ragothaman, M.
AU - Kannan Villalan, A.
AU - Dhanasekaran, A.
AU - Palanisamy, T.
C7 - 112328
DB - Scopus
DO - 10.1016/j.msec.2021.112328
KW - Aminated xanthan gum
Antimicrobial
Collagen
Tissue regeneration
Animals
Hydrogels
Melatonin
Metal Nanoparticles
Silver
Biocompatibility
Drug delivery
Gelation
Hormones
Metal nanoparticles
Morphology
Surface morphology
Tissue
Xanthan gum
antiinfective agent
collagen
melatonin
metal nanoparticle
silver
Bio-hybrids
Hybrid hydrogels
Hydrogel system
Pathogenic infections
Property
Skin defects
Synthesised
Tissue defects
animal
hydrogel
Microorganisms
M3 - Article
PY - 2021
ST - Bio-hybrid hydrogel comprising collagen-capped silver nanoparticles and
melatonin for accelerated tissue regeneration in skin defects
TI - Bio-hybrid hydrogel comprising collagen-capped silver nanoparticles and
85111009070&doi=10.1016%2fj.msec.2021.112328&partnerID=40&md5=e7fe05a1b2ae11b49728f
a030dafd443
VL - 128
ID - 5177
ER -
TY - JOUR
AB - Hydrogel-based drug delivery systems have emerged as a promising platform for
chronic tissue defects owing to their inherent ability to inhibit pathogenic
infection and accelerate rapid tissue regeneration. Here, we fabricated a stable
bio-hybrid hydrogel system comprising collagen, aminated xanthan gum, bio-capped
silver nanoparticles and melatonin with antimicrobial, antioxidant and anti-
inflammatory properties. Highly colloidal bio-capped silver nanoparticles were
synthesized using collagen as a reducing cum stabilizing agent for the first time
while aminated xanthan gum was synthesized using ethylenediamine treatment on
xanthan gum. The synthesized bio-hybrid hydrogel exhibits better gelation, surface
morphology, rheology and degelation properties. In vitro assessment of bio-hybrid
hydrogel demonstrates excellent bactericidal efficiency against both common wound
and multidrug-resistant pathogens and biocompatibility properties. In vivo animal
studies demonstrate rapid tissue regeneration, collagen deposition and angiogenesis
at the wound site predominantly due to the synergistic effect of silver
nanoparticles and melatonin in the hydrogel. This study paves the way for
developing biologically functional bio-nano hydrogel systems for promoting
effective care for various ailments, including infected chronic wounds.
AN - WOS:000691787900005
AU - Ragothaman, M.
AU - Villalan, A. K.
AU - Dhanasekaran, A.
AU - Palanisamy, T.
C6 - JUL 2021
C7 - 112328
DA - SEP
DO - 10.1016/j.msec.2021.112328
PY - 2021
SN - 0928-4931
1873-0191
ST - Bio-hybrid hydrogel comprising collagen-capped silver nanoparticles and
TI - Bio-hybrid hydrogel comprising collagen-capped silver nanoparticles and
VL - 128
ID - 5878
ER -
TY - JOUR
AB - In human immunodeficiency virus type 1 (HIV-1)-infected women, oral or
injectable progesterone containing contraceptive pills may enhance HIV-1
acquisition in vivo, and the mechanism by which this occurs is not fully
understood. In developing countries, Herpes simplex virus type-2 (HSV-2) co-
infection has been shown to be a risk for increase of HIV-1 acquisition and, if co-
infected women use progesterone pills, infections may increase several fold. In
this study, we used an in vitro cell culture system to study the effects of
progesterone on HIV-1 replication and to explore the molecular mechanism of
progesterone effects on infected cells. In our in vitro model, CEMss cells
(lymphoblastoid cell line) were infected with either HIV-1 alone or coinfected
with HSV-2. HIV-1 viral load was measured with and without sex hormone treatment.
Progesterone-treated cells showed an increase in HIV-1 viral load (1411.2pg/mL)
compared with cells without progesterone treatment (993.1 pg/mL). Increased cell
death was noted with HSV-2 co-infection and in progesterone-treated cells. Similar
observations were noted in peripheral blood mononuclear cells (PBMC) cells derived
from three female donors. Progesterone-treated cells also showed reduced antiviral
efficacy. Inflammatory cytokines and associations with biomarkers of disease
progression were explored. Progesterone upregulated inflammatory cytokines and
chemokines conversely and downregulated anti- apoptotic Bcl-2 expression. Nuclear
protein analysis by electrophoretic mobility shift assay showed the association of
progesterone with progesterone response element (PRE), which may lead to
downregulation of Bcl-2. These data indicate that progesterone treatment enhances
HIV-1 replication in infected cells and coinfection with HSV-2 may further fuel
this process.
AN - WOS:000394335000007
AU - Ragupathy, V.
AU - Xue, W.
AU - Tan, J.
AU - Devadas, K.
AU - Gao, Y. M.
AU - Hewlett, I.
DA - OCT
DO - 10.1530/JME-16-0138
IS - 3
PY - 2016
SN - 0952-5041
1479-6813
SP - 185-199
ST - Progesterone augments cell susceptibility to HIV-1 and HIV-1/HSV-2 co-
infections
T2 - JOURNAL OF MOLECULAR ENDOCRINOLOGY
TI - Progesterone augments cell susceptibility to HIV-1 and HIV-1/HSV-2 co-
infections
VL - 57
ID - 6563
ER -
TY - JOUR
AB - Bisphenol A ( BPA ) is chemical compound found in the environment and used in
the manufacturing of plastic, which known as deleterious part of different body
parts. Salvia officinalis ( SO ) which is perennial round shrub in the family of
Labiatae /Lamiaceae. It’s has potent antioxidant and anti-inflammatory effects. The
present study was conducted to evaluate the protective role of SO as nanoparticles
against harmful effect of BPA in female rats. Eighteen adult female rats were used
and divided equally and randomly into three groups as following: first group
received DMSO orally and served as control group while second and third groups were
gavaged either with BPA alone or in combination with SOSNP respectively, all the
treatments extend 30 days. The result revealed harm effect of BPA by significantly
increase the level of estrogen, AST, ALT, ALP, urea and creatinine with
significantly reduction in the FSH and LH. SOSNPs show ameliorative effect on all
above parameters. © 2020, Indian Journal of Forensic Medicine and Toxicology. All
rights reserved.
AU - Raheem, H. A.
AU - Mousa, R. F.
AU - Hassan, A. H.
DB - Scopus
IS - 2
KW - Bisphenol A
FSH
LH
Salvia officinalis
4,4' isopropylidenediphenol
antioxidant
creatinine
dimethyl sulfoxide
estrogen
follitropin
luteinizing hormone
Salvia officinalis extract silver nanoparticle
silver nanoparticle
unclassified drug
urea
adult
animal experiment
animal model
animal tissue
Article
blood sampling
cell damage
controlled study
female
kidney function
liver function
nephrotoxicity
nonhuman
oxidative stress
rat
toxicity
M3 - Article
PY - 2020
SP - 852-857
ST - Evaluation protective role of salvia officinalis silver nanoparticles against
toxic effect of bisphenol a in female rats
T2 - Indian Journal of Forensic Medicine and Toxicology
TI - Evaluation protective role of salvia officinalis silver nanoparticles against
toxic effect of bisphenol a in female rats
85087394103&partnerID=40&md5=853392813e22edb34ffab74f15a4df07
VL - 14
ID - 5334
ER -
TY - JOUR
AB - Hypericum perforatum is native to parts of Europe and Asia but has spread
worldwide as a cosmopolitan invasive weed, including to temperate regions of India,
China, Canada, Africa, and the United States. The aim of this study was to overview
its therapeutic effects. This review article was carried out by searching studies
in PubMed, Medline, Web of Science, and Iran Medex databases. The initial search
strategy identified about 98 references. In this study, 42 studies was accepted for
further screening and met all our inclusion criteria [in English, full text,
therapeutic effects of Hypericum perforatum and dated mainly from the year 1987 to
2016.The search terms were "Hypericum perforatum", lemon balm, "therapeutic
properties", "pharmacological effects". It is commonly used for antimicrobial
effect, neuroprotective effect, anti-depressive effect, antioxidant effect,
menopause, dental practice, anti-inflammatory, wound healing effect, anti-cancer
effect, anti-herpes effect, phototoxicological effect. Hypericum perforatum is
widely used for therapeutic and non-therapeutic purposes that trigger its
significant value. Various combinations and numerous medicinal properties of its
extract, oil, and leaves demand further and more studies about the other useful and
unknown properties of this multipurpose plant.
AU - Rahimi, R.
AU - Kiani, S.
DB - Scopus
IS - 9
KW - Alternative and complementary medicine
Hypericum perforatum
Pharmacognosy
Phytochemicals
Therapeutic effects
alpha tocopherol
antidepressant agent
Arnica montana extract
hyperforin
hypericin
Hypericum perforatum extract
neem oil
placebo
quercetin
sertraline
sulfadiazine silver
actinic keratosis
antidepressant activity
Article
basal cell carcinoma
Bowen disease
burn
depression
desquamation
drug efficacy
experimental surgical wound
head and neck cancer
Herpes simplex virus 1
Herpes simplex virus 2
human
major depression
menopausal syndrome
neuroprotection
nonhuman
phototoxicity
plant leaf
skin toxicity
tooth pain
wound healing
M3 - Article
PY - 2016
SP - 237-241
ST - Chemical compound and therapeutic effects of Hypericum perforatum
T2 - Der Pharmacia Lettre
TI - Chemical compound and therapeutic effects of Hypericum perforatum
84976274997&partnerID=40&md5=542ad34e1e43b934a547af6d67c810d9
VL - 8
ID - 5643
ER -
TY - JOUR
AB - M2 polarization of macrophages is predominant in case of tumors and some
other infectious diseases for disease progression. Repolarization of the M2
phenotype to the M1 state may be required to cure diseases. Hence, it is of great
interest to find out a material that would repolarize the M2 phenotype to the M1
state. Herein, the arabinogalactan protein from Andrographis paniculata (ApAGP) was
used to prepare a silver nanoparticle-ApAGP (SNP-ApAGP) bioconjugate, which was
characterized via UV-vis spectroscopy, zeta potential analysis, FT-IR spectroscopy,
and HR-TEM. Studies suggest that SNP-ApAGP (2.5 mu g mL(-1)) up-regulates ROS
generation, NO generation, and pro-inflammatory cytokine release (IL-12, IFN-gamma,
TNF-alpha, and IL-6). SNP-ApAGP also down-regulates the arginase-1 activity and
anti-inflammatory cytokine release (IL-4 & IL-10) in M0, M1, and M2-polarized
peritoneal macrophages in vitro. Therefore, SNP-ApAGP induces M1 polarization in M0
macrophages, enhances the proinflammatory activity of the M1 phenotype, and can
also repolarize M2 macrophages into the M1 phenotype. Therefore, SNP-ApAGP could be
used for treating various infectious diseases and cancers where repolarization of
M2 macrophages may be required to cure the disease.
AN - WOS:000401626800006
AU - Raja, M. R. C.
AU - Kumar, V. V.
AU - Srinivasan, V.
AU - Selvaraj, S.
AU - Radhakrishnan, N.
AU - Mukundan, R.
AU - Raghunandan, S.
AU - Anthony, S. P.
AU - Mahapatra, S. K.
DA - MAY 21
DO - 10.1039/c6tb02095j
IS - 19
PY - 2017
SN - 2050-750X
2050-7518
SP - 3511-3520
ST - ApAGP-fabricated silver nanoparticles induce amendment of murine macrophage
polarization
TI - ApAGP-fabricated silver nanoparticles induce amendment of murine macrophage
polarization
VL - 5
ID - 6195
ER -
TY - JOUR
AB - Apoptosis, a physiological mechanism of highly orchestrated cell death, can
be initiated by extracellular and intracellular mechanisms that trigger a complex
machinery of proapoptotic proteases and mitochondrial changes, leading to the
activation of specific endonucleases and DNA fragmentation. The present study was
undertaken to elucidate a mechanism underlying the inhibitory effect of
biosynthesised silver nanoparticle on TNF-α induced NF-κB nuclear translocation in
prostate cancer PC- 3 cells. The cell cycle analysis of Prostate cancer PC-3 cells
was examined by flow cytometry by using annexin V-FITC/PI staining. Effect of
silver nanoparticles in oxidative stress ROS, Effect of biosynthesized silver
nanoparticle on apoptosis in human prostate cancer cell line and apoptotic
induction of TNF-α and NF-κB expression was studied by Flow cytometry in Prostate
cancer PC-3 cell line. From the results it was observed that biosynthesized silver
nanoparticle inhibits the cellular growth of human prostate cancer PC-3 cells and
induces apoptosis. The ROS levels generated in response to silver nanoparticles
were significantly higher in treated PC-3 cells than the control. The result
indicates that cell death is mediated by ROS production, which might alter the
cellular redox status, and it is a potential reason for cell death. Apoptosis of
the silver nanoparticle treated PC-3 cells was accompanied by a reduction in the
percentage of cells in G0/G1 phase and an increase in the percentage of G2/M phase
cells, indicating cell cycle arrest at G2/M phase, and transcription factor NF-κB
plays an essential role in inflammation and cancer. The activation of NF-κB in
response to inflammatory cytokine such as TNF-α promotes nuclear migration to
enable DNA-binding activity and facilitate target genes expression. © 2014 Society
for Industrial and Applied Mathematics and American Statistical Association.
AU - Rajathi, K.
AU - Leneeygreen, K. B.
AU - Suja, S.
DB - Scopus
DO - 10.37285/ijpsn.2021.14.2.5
IS - 2
KW - Apoptosis
Flow cytometry
NF-kB
Prostate cancer
Silver nanoparticle
TNF
lipocortin 5
propidium iodide
silver nanoparticle
apoptosis
Article
biosynthesis
cancer inhibition
cell growth
cell migration
controlled study
cytotoxicity
DNA binding
DNA content
DNA fragmentation
drug effect
drug mechanism
flow cytometry
fluorescence intensity
gene translocation
human
human cell
oxidative stress
PC-3 [Human prostate carcinoma] cell line
percentage of cells in G0/G1 phase
percentage of cells in G2/M phase
priority journal
prostate cancer
protein expression
treatment response
M3 - Article
PY - 2021
SP - 5396-5405
ST - Mechanism underlying the inhibitory effect of biosynthesized silver
nanoparticle on TNF-α induced NF-κB nuclear translocation in prostate cancer cells
T2 - International Journal of Pharmaceutical Sciences and Nanotechnology
TI - Mechanism underlying the inhibitory effect of biosynthesized silver
nanoparticle on TNF-α induced NF-κB nuclear translocation in prostate cancer cells
85108918316&doi=10.37285%2fijpsn.2021.14.2.5&partnerID=40&md5=a24089d82a59c60082625
9c61bac00be
VL - 14
ID - 5239
ER -
TY - JOUR
AB - Naegleria fowleri (N. fowleri) causes primary amoebic meningoencephalitis
(PAM) which almost always results in death. N. fowleri is also known as "brain-
eating amoeba"due to its literal infestation of the brain leading to an
inflammatory response in the brain tissues. Currently, there is no single drug that
is available to treat PAM, and most treatments are combinations of antifungal,
anticancer, and anti-inflammatory drugs. Recently nanotechnology has gained
attention in chemotherapeutic research converging on drug delivery, while oleic
acid (OA) has shown positive effects on the human immune system and inflammatory
processes. In continuation of our recent research in which we reported the effects
of oleic acid conjugated with silver nanoparticles (OA-AgNPs) against free-living
amoeba Acanthamoeba castellanii, in this report, we show their antiamoebic effects
against N. fowleri. OA alone and its nanoconjugates were tested against the amoeba
by using amoebicidal and host cell cytopathogenicity assays. Trypan blue exclusion
assay was used to determine cell viability. The results revealed that OA-AgNPs
exhibited significantly enhanced antiamoebic effects (P < 0.05) against N. fowleri
as compared to OA alone. Evidently, lactate dehydrogenase release shows reduced N.
fowleri-mediated host cell cytotoxicity. Based on our study, we anticipate that
further studies on OA-AgNPs could potentially provide an alternative treatment of
PAM. Copyright © 2019 American Chemical Society.
AU - Rajendran, K.
AU - Anwar, A.
AU - Khan, N. A.
AU - Aslam, Z.
AU - Raza Shah, M.
AU - Siddiqui, R.
DB - Scopus
DO - 10.1021/acschemneuro.9b00289
IS - 16
KW - Brain-eating amoeba
Chemotherapy
Naegleria fowleri
Oleic acid
Primary amoebic meningoencephalitis (PAM)
Amebicides
Amphotericin B
Central Nervous System Protozoal Infections
Humans
Metal Nanoparticles
Oleic Acid
Silver
amphotericin B
nanocoating
nanoconjugate
oleic acid
silver nanoparticle
sodium borohydride
trypan blue
antiamebic agent
metal nanoparticle
silver
amoeba (life cycle stage)
amoebicidal activity
antiamoebic activity
Article
cell viability
conjugate
controlled study
cytopathogenic effect
cytotoxicity assay
enzyme release
host cell
in vitro study
nonhuman
primary amebic meningoencephalitis
priority journal
suspension
central nervous system infection
human
M3 - Article
PY - 2020
SP - 2431-2437
ST - Oleic Acid Coated Silver Nanoparticles Showed Better in Vitro Amoebicidal
Effects against Naegleria fowleri than Amphotericin B
T2 - ACS Chemical Neuroscience
TI - Oleic Acid Coated Silver Nanoparticles Showed Better in Vitro Amoebicidal
85070791196&doi=10.1021%2facschemneuro.9b00289&partnerID=40&md5=f2c217ea29e091b7beb
b1d8f7bebba74
VL - 11
ID - 5314
ER -
TY - JOUR
AB - Naegleria fowleri (N. fowleri) causes primary amoebic meningoencephalitis
(PAM) which almost always results in death. N. fowleri is also known as "brain-
eating amoeba" due to its literal infestation of the brain leading to an
inflammatory response in the brain tissues. Currently, there is no single drug that
is available to treat PAM, and most treatments are combinations of antifungal,
anticancer, and anti-inflammatory drugs. Recently nanotechnology has gained
attention in chemotherapeutic research converging on drug delivery, while oleic
acid (OA) has shown positive effects on the human immune system and inflammatory
processes. In continuation of our recent research in which we reported the effects
of oleic acid conjugated with silver nanoparticles (OA-AgNPs) against free-living
amoeba Acanthamoeba castellanii, in this report, we show their antiamoebic effects
against N. fowleri. OA alone and its nanoconjugates were tested against the amoeba
by using amoebicidal and host cell cytopathogenicity assays. Trypan blue exclusion
assay was used to determine cell viability. The results revealed that OA-AgNPs
exhibited significantly enhanced antiamoebic effects (P < 0.05) against N. fowleri
as compared to OA alone. Evidently, lactate dehydrogenase release shows reduced N.
fowleri-mediated host cell cytotoxicity. Based on our study, we anticipate that
further studies on OA-AgNPs could potentially provide an alternative treatment of
PAM.
AN - WOS:000563072600011
AU - Rajendran, K.
AU - Anwar, A.
AU - Khan, N. A.
AU - Aslam, Z.
AU - Shah, M. R.
AU - Siddiqui, R.
DA - AUG 19
DO - 10.1021/acschemneuro.9b00289
IS - 16
PY - 2020
SN - 1948-7193
SP - 2431-2437
ST - Oleic Acid Coated Silver Nanoparticles Showed Better in Vitro Amoebicidal
T2 - ACS CHEMICAL NEUROSCIENCE
TI - Oleic Acid Coated Silver Nanoparticles Showed Better in Vitro Amoebicidal
VL - 11
ID - 5972
ER -
TY - JOUR
AB - Objective Delonix regia(L.) (Caesalpiniaceae) is one of the plants that have
been used for centuries to prevent cancer.D. regiahas been described as a strong
anti-inflammatory, antibacterial, and analgesic. Also, the cytotoxicity of this
plant has been proved due to its flavonoid compounds. Besides, silver (Ag)
nanoparticles have been proved to be promising in cancer treatment. Methods The
synergistic effect of medicinal plant extracts with silver antibacterial
nanoparticles on cancer cell inhibition was investigated. Cytotoxic effect ofD.
regiaextract with silver NPs on MCF-7 and Panc-1 cancer cells was investigated by
cell viability analysis, morphological changes, apoptosis induction, and TUNEL
assay. Results Results showed the cells treated withD. regiaextract containing
AgNPs exhibited a considerable reduction in viability percent in comparison with
control cells. Analysis of MTT demonstrated that the IC(50)value ofD. regiaextract
and AgNPs on both cell lines observed in 0.5 mg/mL for 24 h. The results
demonstrated that the maximum inhibition of both cell lines growth was observed in
1.5 mg/L and 0.2 mg/L in the presence withD. regiaand AgNPs, respectively. Also,
morphological characteristics of cells indicated thatD. regiaextract and silver NPs
induce cell death by apoptosis and it is confirmed by TUNEL assay. Conclusion
Interestingly,D. regiaextract and Ag NPs had no significant cytotoxicity against
normal cells. It can be concluded thatDelonix regiaplant and silver nanoparticles
have an inhibitory potential on two cancer cell lines, but in the use of synergism
ofD. regiaextract and AgNPs, the induction of apoptosis in MCF-7 and Panc-1 was
increased. Interestingly, in normal cells, no significant negative effect was
observed as control.
AN - WOS:000564490000001
AU - Rajiri, M. S.
AU - Aminsalehi, M.
AU - Shahbandeh, M.
AU - Maleki, A.
AU - Jonoubi, P.
AU - Rad, A. C.
C6 - AUG 2020
DA - MAR
DO - 10.1007/s13530-020-00067-1
IS - 1
PY - 2021
SN - 2005-9752
2233-7784
SP - 45-56
ST - Anticancer and therapeutic potential ofDelonix regiaextract and silver
nanoparticles (AgNPs) against pancreatic (Panc-1) and breast (MCF-7) cancer cell
T2 - TOXICOLOGY AND ENVIRONMENTAL HEALTH SCIENCES
TI - Anticancer and therapeutic potential ofDelonix regiaextract and silver
VL - 13
ID - 5984
ER -
TY - JOUR
AB - Key message Atropa acuminataaqueous leaf extract biosynthesized silver
nanoparticles showed strong antioxidant, anticancerous (HeLa cells) and anti-
inflammatory activities. Besides, this bio syn-AgNP also proved effective against
mosquito vectors causing malaria, dengue and filariasis. Present study highlights
eco-friendly and sustainable approach for the synthesis of silver nanoparticles
(AgNP) using aqueous leaf extract ofA. acuminata, a critically endangered medicinal
herb. The addition of 1 mM silver nitrate to aqueous leaf extract resulted in the
synthesis of AgNP when solution was heated at 60 degrees C for 30 min at pH 7.
Absorption band at 428 nm, as shown by UV-Vis spectroscopy confirmed the synthesis
of AgNP. XRD patterns revealed the crystalline nature of AgNP and TEM analysis
showed that most of the nanoparticles were spherical in shape. Zeta potential of
AgNP was found to be - 33.5 mV which confirmed their high stability. FT-IR
investigations confirmed the presence of different functional groups involved in
the reduction and capping of AgNP. The synthesized AgNP showed effective DPPH
(IC50-16.08 mu g/mL), H2O2(IC50-25.40 mu g/mL), and superoxide (IC50-21.12 mu g/mL)
radical scavenging activities. These plant-AgNP showed significant inhibition of
albumin denaturation (IC50-12.98 mu g/mL) and antiproteinase activity (IC50-18.401
mu g/mL). Besides, biosynthesized AgNP were found to have strong inhibitory effect
against a cervical cancer (HeLa) cell line (IC50-5.418 mu g/mL) as well as
larvicidal activity against 3rd instar larvae ofAnopheles stephensi(LC50-18.9 ppm,
LC90-40.18 ppm),Aedes aegypti(LC50-12.395 ppm, LC90-36.34 ppm) andCulex
quinquefasciatus(LC50-17.76 ppm, LC90-30.82 ppm) and were found to be non-toxic
against normal cell line (HEK 293), and a non-target organism (Mesocyclops
thermocyclopoides). This is the first report on the synthesis of AgNP using aqueous
leaf extract ofA. acuminata, validating their strong therapeutic potential.
AN - WOS:000541340300007
AU - Rajput, S.
AU - Kumar, D.
AU - Agrawal, V.
DA - JUL
DO - 10.1007/s00299-020-02539-7
IS - 7
PY - 2020
SN - 0721-7714
1432-203X
SP - 921-939
ST - Green synthesis of silver nanoparticles using Indian Belladonna extract and
their potential antioxidant, anti-inflammatory, anticancer and larvicidal
activities
T2 - PLANT CELL REPORTS
TI - Green synthesis of silver nanoparticles using Indian Belladonna extract and
their potential antioxidant, anti-inflammatory, anticancer and larvicidal
activities
VL - 39
ID - 5937
ER -
TY - JOUR
AB - The response of a murine macrophage cell line exposed to a library of seven
metal and metal oxide nanoparticles was evaluated via High Throughput Screening
(HTS) assay employing luciferase-reporters for ten independent toxicity-related
signaling pathways. Similarities of toxicity response among the nanoparticles were
identified via Self-Organizing Map (SOM) analysis. This analysis, applied to the
HTS data, quantified the significance of the signaling pathway responses (SPRs) of
the cell population exposed to nanomaterials relative to a population of untreated
cells, using the Strictly Standardized Mean Difference (SSMD). Given the high
dimensionality of the data and relatively small data set, the validity of the SOM
clusters was established via a consensus clustering technique. Analysis of the SPR
signatures revealed two cluster groups corresponding to (i) sublethal pro-
inflammatory responses to Al 2O3, Au, Ag, SiO2 nanoparticles possibly related to
ROS generation, and (ii) lethal genotoxic responses due to exposure to ZnO and Pt
nanoparticles at a concentration range of 25-100 μg/mL at 12 h exposure. In
addition to identifying and visualizing clusters and quantifying similarity
measures, the SOM approach can aid in developing predictive quantitative-structure
relations; however, this would require significantly larger data sets generated
from combinatorial libraries of engineered nanoparticles. © 2011 American Chemical
Society.
AU - Rallo, R.
AU - France, B.
AU - Liu, R.
AU - Nair, S.
AU - George, S.
AU - Damoiseaux, R.
AU - Giralt, F.
AU - Nel, A.
AU - Bradley, K.
AU - Cohen, Y.
DB - Scopus
DO - 10.1021/es103606x
IS - 4
KW - Animals
Cell Line
Luciferases
Macrophages
Metal Nanoparticles
Mice
Nanostructures
Oxides
Signal Transduction
Murinae
Cell culture
Cell proliferation
Conformal mapping
Metallic compounds
Metals
Nanoparticles
Pigments
Platinum
Population statistics
Signaling
Silicon compounds
Toxicity
Zinc oxide
aluminum oxide
gold
metal nanoparticle
metal oxide nanoparticle
platinum
silicon dioxide
silver
unclassified drug
zinc oxide
luciferase
nanomaterial
oxide
Cell populations
Cell signaling
Combinatorial library
Concentration ranges
Consensus clustering
Data sets
Genotoxic
High dimensionality
High-throughput screening
Murine macrophages
Nano-materials
Pt nanoparticles
Self organizing
Signaling pathways
Similarity measure
Small data set
ZnO
cluster analysis
data set
metal
nanotechnology
toxicity
animal cell
article
audiovisual equipment
cell communication
cell population
controlled study
cytotoxicity
genotoxicity
macrophage
nonhuman
self organizing map analysis
signal transduction
animal
cell line
chemistry
drug effects
metabolism
mouse
Metal analysis
M3 - Article
PY - 2011
SP - 1695-1702
ST - Self-organizing map analysis of toxicity-related cell signaling pathways for
metal and metal oxide nanoparticles
TI - Self-organizing map analysis of toxicity-related cell signaling pathways for
metal and metal oxide nanoparticles
79951650762&doi=10.1021%2fes103606x&partnerID=40&md5=edded015d77f3fd65eaf399d3c22d3
5d
VL - 45
ID - 5757
ER -
TY - JOUR
AB - The present study was aimed at biosynthesis of silver nanoparticles (AgNPs)
using ethanolic extract of rose (Rosa indica) petals and testing their potential
antibacterial activity using selective human pathogenic microbes, anticancer
activity using human colon adenocarcinoma cancer cell line HCT 15 as well as anti-
inflammatory activity using rat peritoneal macrophages in vitro. The biologically
synthesized AgNPs were also characterized by UV-visible spectroscopy, scanning
electron microscopy (SEM), transmission electron microscopy (TEM), Fourier
transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX)
and X-ray diffraction (XRD). The characterized AgNPs showed an effective
antibacterial activity against Gram negative (Escherichia coli, Klebsiella
pneumoniae) than Gram positive (Streptococcus mutans, Enterococcus faecalis)
bacteria. MU assay, analysis of nuclear morphology, mRNA expression of Bcl-2, Bax
and protein expression of caspase 3 as well as 9, indicated potential anticancer
activity. In addition, green synthesized AgNPs also attenuated cytotoxicity,
nuclear morphology and free radical generation (O-2(-) and NO) by rat peritoneal
macrophages in vitro. The results of our study show the potential green synthesis
of silver nanoparticles in mitigating their toxicity while retaining their
antibacterial activities. (C) 2014 Elsevier B.V. All rights reserved.
AN - WOS:000348955300015
AU - Ramar, M.
AU - Manikandan, B.
AU - Raman, T.
AU - Arunagirinathan, K.
AU - Prabhu, N. M.
AU - Basu, M. J.
AU - Perumal, M.
AU - Palanisamy, S.
AU - Munusamy, A.
DA - MAR 5
DO - 10.1016/j.saa.2014.10.043
PY - 2015
SN - 1386-1425
1873-3557
SP - 120-129
ST - Biosynthesis of silver nanoparticles using ethanolic petals extract of Rosa
activities
T2 - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
TI - Biosynthesis of silver nanoparticles using ethanolic petals extract of Rosa
activities
VL - 138
ID - 5841
ER -
TY - JOUR
AB - Methotrexate (MTX) is an antifolate metabolite that is used in the treatment
of various autoimmune diseases, malignancies, and inflammatory disorders. In
addition to the well-characterized side effects such as hepatotoxicity and
myelosuppression, it can also rarely cause a variety of cutaneous manifestations
due to acute toxicity. We are presenting case series of three patients of MTX
toxicity. All three cases presented with acute ulceration and pain over the
psoriatic plaques in addition to mucosal involvement. They were all given
injectable folinic acid. Two out of the three patients died and one of them
recovered. Although low-dose MTX appears to be relatively safe, acute MTX toxicity
is a life-threatening emergency that can occur for which greater awareness of this
condition is needed for its prevention, early diagnosis, and management. © 2022
Journal of the Egyptian Women's Dermatologic Society.
AU - Ramesh, H.
AU - Kanathur, S.
AU - Loganathan, E.
AU - Somashekhar, S.
DB - Scopus
DO - 10.4103/jewd.jewd_47_21
IS - 2
KW - methotrexate toxicity
overdosing
psoriasis
antibiotic agent
folinic acid
methotrexate
sulfadiazine silver
acute toxicity
adult
Article
bleeding
blood cell count
case report
clinical article
erosion
fever
human
jaundice
low drug dose
male
middle aged
mouth lesion
septic shock
skin care
skin examination
thrombocyte transfusion
ulcer
ulcer healing
M3 - Article
PY - 2022
SP - 137-140
ST - Acute methotrexate toxicity in patients with psoriasis: case series
T2 - Journal of the Egyptian Women's Dermatologic Society
TI - Acute methotrexate toxicity in patients with psoriasis: case series
85130867339&doi=10.4103%2fjewd.jewd_47_21&partnerID=40&md5=4d1f75f1eb8881b9f0315ae2
7b15af54
VL - 19
ID - 5073
ER -
TY - JOUR
AB - Nanotechnology is an evolving interdisciplinary field of research
interspersing material science and nanobiotechnology. Nanoparticles are studied
extensively for their specific catalytic, magnetic, electronic, optical,
antimicrobial, theranostic, diagnosis, wound healing, and anti-inflammatory
properties. ZnO nanoparticles (NPs) have many applications owing to their unique
characteristics, which include low cost, nontoxicity, abundance in nature, and the
ability to prepare compounds with varying morphologies having different properties.
The main aim of the study is to biosynthesis of ZnO nanoparticles coated with
silver from the aqueous extract of Ganoderma lucidum (Curtis) P. Karst and to
evaluate its antidiabetic potential by performing alpha-glucosidase inhibition and
alpha-amylase inhibition assays and to evaluate the anticancer potential by
cytotoxicity (MTT) assay against human breast cancer MDA-MB 231 cell lines. The
biosynthesis of ZnO nanoparticles coated with Ag was characterized by UV-vis
spectroscopy, Fourier transform infrared spectroscopy, energy dispersive X-ray
analysis, scanning electron microscope, and transmission electron microscopy. An
increasing concentration in the biosynthesized ZnO nanoparticles coated with Ag
produces strong antidiabetic activity through enzyme inhibition effect and
anticancer activity through the reduction of cell viability. The present study
recommended that the "Biological"method of biological nanoparticle production is a
promising approach that allows synthesis in aqueous conditions, with low energy
requirements and low costs. In the future, the mycosynthesized nanoparticles might
be used in the medical arena to treat and prevent diseases. © 2022 D. S. Ranjith
Santhosh Kumar et al.
AU - Ranjith Santhosh Kumar, D. S.
AU - Elango, N.
AU - Selvaraju, G. D.
AU - Matthew, P. A.
AU - Palanisamy, S.
AU - Cho, H.
AU - Al Khattaf, F. S.
AU - Hatamleh, A. A.
AU - Roy, A. D.
C7 - 2798532
DB - Scopus
DO - 10.1155/2022/2798532
KW - Biochemistry
Biosynthesis
Cell culture
Costs
Diagnosis
Energy dispersive X ray analysis
Enzyme activity
Enzyme inhibition
Metal nanoparticles
Nanomagnetics
Anticancer potentials
Antidiabetic
Ganoderma Lucidum
In-vitro
Interdisciplinary fields
ITS evaluation
Low-costs
Material science
ZnO nanoparticles
M3 - Article
PY - 2022
ST - Mycosynthesis of Zinc Oxide Nanoparticles Coated with Silver using Ganoderma
T2 - Journal of Nanomaterials
TI - Mycosynthesis of Zinc Oxide Nanoparticles Coated with Silver using Ganoderma
85145967884&doi=10.1155%2f2022%2f2798532&partnerID=40&md5=b212815d66479ef4da4802bdd
e6e528f
VL - 2022
ID - 5080
ER -
TY - JOUR
AB - Ten materials used as root canal sealers were evaluated experimentally. These
materials were (1) zinc oxide and eugenol, (2) AH-26, (3) Diaket, (4) Proco-Sol
radiopaque silver cement, (5) Proco-Sol nonstaining root canal cement, (6) Kerr
sealer, (7) Kloroperka, (8) N2, (9) N2 Medical, and (10) Mynol root canal sealer.
Four methods of testing were utilized, as follows: 1. A. Animal study, in which the
subcutaneous connective tissue of the rat was observed for inflammatory response to
implants of the materials tested. 2. B. Tissue-culture toxicity study in which
varying dilutions of the test materials were placed on tissue cultures of HeLa
cells and the degree of toxicity noted. 3. C. A bacteriologic study wherein the
degree of growth inhibition of the test materials to known organisms was measured.
4. D. A selected-material animal study in which four of the test materials were
placed in the conjunctival sac of the eye of the rabbit. This structure was then
observed for degree of inflammation. The materials were found to be fairly
consistent from study to study. Some variables were noted, but for the most part
the materials exhibited a fairly uniform response within each test group when all
methods were compared. © 1964.
AU - Rappaport, H. M.
AU - Lilly, G. E.
AU - Kapsimalis, P.
DB - Scopus
DO - 10.1016/0030-4220(64)90480-3
IS - 6
KW - Bacteria
Dental Materials
Inflammation
Pathology
Pharmacology
Rabbits
Rats
Research
Root Canal Therapy
Toxicology
BACTERIA
DENTAL MATERIALS
EXPERIMENTAL LAB STUDY
INFLAMMATION
PATHOLOGY
PHARMACOLOGY
RABBITS
RATS
ROOT CANAL THERAPY
TOXICOLOGIC REPORT
ZINC OXIDE-EUGENOL CEMENT
zinc oxide eugenol
article
bacterium
endodontics
inflammation
pathology
pharmacology
rabbit
rat
research
toxicology
M3 - Article
PY - 1964
SP - 785-802
ST - Toxicity of endodontic filling materials
T2 - Oral Surgery, Oral Medicine, Oral Pathology
TI - Toxicity of endodontic filling materials
0008579430&doi=10.1016%2f0030-4220%2864%2990480-
3&partnerID=40&md5=646205536a602a51ebb660e33d7b2b99
VL - 18
ID - 5822
ER -
TY - JOUR
AB - Hepatocellular carcinoma is the most ordinary type of liver cancer and most
commonly appears in a patient with chronic viral hepatitis and with cirrhosis.
Lipopolysaccharide motivates the hepatocyte cells and increases the production
inducible cytokine with the high production of nitric oxide and reactive oxygen
species. In this study, the rate change iNOS searched by adding lipopolysaccharide
and the effect of inhibiting this inflammatory enzyme by L-NAME and combined effect
of this in HepG2 cancer cells was assessed. Salmonella enteritidis
lipopolysaccharide is extracted by methanol-chloroform method and silver stained
SDS-PAGE electrophoresis bands. Four treatment groups of Human liver hepatocellular
carcinoma cell lines stimulated with 100ng/ml lipopolysaccharide, 500 mu M L-NAME
as inhibitor and were incubated for 12 and 24 hours. Variables including both
increase and decrease inflammatory factor, iNOS, was assayed. The obtained results
showed that initial activity of iNOS in not stimulated in HepG2 cell, 0.902U/ml
after stimulation with lipopolysaccharide for 12 and 24hours (0.491U/ml) and
(0.433U/ml) decreased and the effect of this inhibitor was also studied. The data
showed that reducing iNOS in Human liver hepatocellular carcinoma cell lines
correlated with the cell density and duration of incubation with LPS. Inhibition of
enzyme associated with inflammation, with inhibitor substances; L-NAME, observed as
well as lipopolysaccharide. We can potentially design drugs to treat a variety
disease and cancer to use.
AN - WOS:000304500700005
AU - Rastegar, H.
AU - Mirzaei, A.
AU - Ashtiani, H. R. A.
AU - Hedayati, M.
AU - Ahmadi, S.
DA - MAR
IS - 1
PY - 2012
SN - 0973-7510
2581-690X
SP - 39-45
ST - The Effect of Extracted Bacterial Salmonella enteritidis Lipopolysaccharide
on Inducible Nitric Oxide Synthase in Human Liver Hepatocellular Carcinoma Cell
Lines in Induction and Inhibition Conditions
T2 - JOURNAL OF PURE AND APPLIED MICROBIOLOGY
TI - The Effect of Extracted Bacterial Salmonella enteritidis Lipopolysaccharide
on Inducible Nitric Oxide Synthase in Human Liver Hepatocellular Carcinoma Cell
Lines in Induction and Inhibition Conditions
VL - 6
ID - 6599
ER -
TY - JOUR
AB - Bioftlm formation is one of the main obstacles that occur during in vivo
implantation, which compromises the implant functionality and patients' health.
This is the inspiration for the development of novel implant materials that contain
broad-spectrum antimicrobial activity, including antibacterial and antifungal, and
enable the local release of antimicrobial agents. Here, multifunctional calcium
phosphate-ionic liquid (IL) materials, possessing antimicrobial and
repair/regeneration features plus injectability, are proposed as implants in
minimally invasive surgery. This approach was based on the loading of 1-alkyl-3-
alkylimidazolium chloride ionic liquids (ILs) (C(n)MImCl (n = 4, 10, 16) and (C-10)
(2)MImCl) during the in situ sol-gel synthesis of calcium phosphates (CaP) and
study of their effects on CaP crystallization and biological properties. Physical,
morphological, and biological investigations were performed to evaluate the
bionanocomposites' properties. The IL N-alkyl chain length influenced the
crystallization of CaP and, consequently, the biological properties, which afforded
bionanocomposites (when loaded with C(16)MImCl or (C-10)(2)MImCl) that, (i) inhibit
both in vitro bacterial and fungal growth; (ii) reduce the in vitro inflammatory
response; (iii) induce osteogenic differentiation in the basal medium of human
mesenchymal stem cells; and (iv) are injectable. This will enable the design of
multifunctional injectable implants with antimicrobial, anti-inflammatory, and
regenerative properties to be used in minimally invasive surgery of bone and
maxillofacial defects.
AN - WOS:000453488900101
AU - Raucci, M. G.
AU - Fasolino, I.
AU - Pastore, S. G.
AU - Soriente, A.
AU - Capeletti, L. B.
AU - Dessuy, M. B.
AU - Giannini, C.
AU - Schrekker, H. S.
AU - Ambrosio, L.
DA - DEC 12
DO - 10.1021/acsami.8b12696
IS - 49
PY - 2018
SN - 1944-8244
SP - 42766-42776
ST - Antimicrobial Imidazolium Ionic Liquids for the Development of Minimal
Invasive Calcium Phosphate-Based Bionanocomposites
TI - Antimicrobial Imidazolium Ionic Liquids for the Development of Minimal
Invasive Calcium Phosphate-Based Bionanocomposites
VL - 10
ID - 6732
ER -
TY - JOUR
AB - Nonsteroidal anti-inflammatory drugs ( NSAIDs) are used extensively as
therapeutic agents, despite their well documented gastrointestinal ( GI) toxicity.
At this time, the mechanisms responsible for NSAID-associated GI damage are
incompletely understood. In this study, we used microarray analysis to generate a
novel hypothesis about cellular mechanisms that underlie the GI toxicity of NSAIDs.
Monolayers of intestinal epithelial cells ( IEC-6) were treated with NSAIDs that
either exhibit ( indomethacin, NS-398 [ N-[2-(cyclohexyloxy)-4-nitrophenyl]-
methanesulfonamide]) or lack ( SC-560[5-(4-chlorphenyl)-1-(4-methoxyphenyl)-3-
(trifluoromethyl)1H-pyrazole]) inhibitory effects on IEC-6 migration. Bioinformatic
analysis of array data identified the calpain cysteine proteases and their
endogenous inhibitor calpastatin as potential targets of NSAIDs shown previously to
retard IEC-6 migration. Accordingly, quantitative real-time reverse transcription
polymerase chain reaction and immunoblotting were performed to assess the effects
of NSAIDs on the expression of mRNA and protein for calpain 8, calpain 2, calpain
1, and calpastatin. In treated IEC-6 monolayers, NS-398 decreased the expression of
mRNA for calpain 2 and calpain 8. Both NS-398 and indomethacin decreased the
protein expression of calpains 8, 2, and 1. None of the NSAIDs affected expression
of calpastatin mRNA or protein. The calpain inhibitors,N-acetyl-Leu-Leu-methioninal
and N-acetyl-Leu-Leu-Nle- CHO, retarded IEC-6 cell migration in a concentration-
dependant fashion, and these inhibitory effects were additive with those of
indomethacin and NS-398. Our experimental results suggest that the altered
expression of calpain proteins may contribute to the adverse effects of NSAIDs on
intestinal epithelial restitution.
AN - WOS:000255013800006
AU - Raveendran, N. N.
AU - Silver, K.
AU - Freeman, L. C.
AU - Narvaez, D.
AU - Weng, K.
AU - Ganta, S.
AU - Lillich, J. D.
DA - MAY
DO - 10.1124/jpet.107.127720
IS - 2
PY - 2008
SN - 0022-3565
SP - 389-399
ST - Drug-induced alterations to gene and protein expression in intestinal
epithelial cell 6 cells suggest a role for calpains in the gastrointestinal
toxicity of nonsteroidal anti-inflammatory agents
T2 - JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
TI - Drug-induced alterations to gene and protein expression in intestinal
epithelial cell 6 cells suggest a role for calpains in the gastrointestinal
toxicity of nonsteroidal anti-inflammatory agents
VL - 325
ID - 6205
ER -
TY - JOUR
AB - Rheumatoid arthritis (RA) is a progressive, inflammatory, autoimmune,
symmetrical polyarticular arthritis. It is characterized by synovial infiltration
and activation of several types of immune cells, culminating in their apoptosis and
antibody generation against "altered" autoantigens. beta 2-microglobulin ( beta
2m)-associated heavy chains (HCs) of HLA antigens, also known as closed conformers
(Face-1), undergo "alteration" during activation of immune cells, resulting in beta
2m-free structural variants, including monomeric open conformers (Face-2) that are
capable of dimerizing as either homodimers (Face-3) or as heterodimers (Face-4).
beta 2m-free HCs uncover the cryptic epitopes that can elicit antibodies (Abs). We
report here the levels of IgM and IgG Abs against both beta 2m and HCs of HLA-E,
HLA-F, and HLA-G in 74 RA patients receiving immunosuppressive drugs. Anti-beta 2m
IgM was present in 20 of 74 patients, whereas anti-beta 2m IgG was found in only 8
patients. Abs against beta 2m would be expected if Abs were generated against beta
2m-associated HLA HCs. The majority of patients were devoid of either anti-beta 2m
IgM or IgG but had Abs against HCs of different HLA-Ib molecules. The paucity of
anti-beta 2m Abs in this cohort of patients suggests that Abs were developed
against beta 2m-free HLA HCs, such as Face-2, Face-3, and Face-4. While 63 of 68
patients had IgG Abs against anti-HLA-F HCs, 36 and 50 patients showed IgG Ab
reactivity against HLA-E and anti-HLA-G HCs, respectively. Evidently, anti-HLA-F HC
Abs are the most predominant anti-HLA-Ib HC IgG Abs in RA patients. The incidence
and intensity of Abs against HLA-E, HLA-F, and HLA-G in the normal control group
were much higher than those observed in RA patients. Evidently, the lower level of
Abs in RA patients points to the impact of the immunosuppressive drugs on these
patients. These results underscore the need for further studies to unravel the
nature of HLA-F variants on activated immune cells and synoviocytes of RA patients.
AN - WOS:001020668700001
AU - Ravindranath, M. H.
AU - Ravindranath, N. M.
AU - Amato-Menker, C. J.
AU - El Hilali, F.
AU - Selvan, S. R.
AU - Filippone, E. J.
AU - Morales-Buenrostro, L. E.
C7 - 26
DA - JUN
DO - 10.3390/antib12020026
IS - 2
PY - 2023
SN - 2073-4468
ST - Antibodies for beta 2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and
HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease
Progression in Rheumatoid Arthritis Patients under Treatment
T2 - ANTIBODIES
TI - Antibodies for beta 2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and
HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease
Progression in Rheumatoid Arthritis Patients under Treatment
VL - 12
ID - 6768
ER -
TY - JOUR
AB - Additive manufacturing (AM) techniques have provided many opportunities for
the rational design of porous metallic biomaterials with complex and precisely
controlled topologies that give rise to unprecedented combinations of mechanical,
physical, and biological properties. These favorable properties can be enhanced by
surface biofunctionalization to enable full tissue regeneration and minimize the
risk of implant-associated infections (IAIs). There is, however, an increasing need
to investigate the immune responses triggered by surface biofunctionalized AM
porous metals. Here, we studied the immunomodulatory effects of AM porous titanium
(Ti-6Al-4V) printed using selective laser melting, and of two additional groups
consisting of AM implants surface biofunctionalized using plasma electrolytic
oxidation (PEO) with/without silver nanoparticles. The responses of human primary
macrophages and human mesenchymal stromal cells (hMSCs) were studied in terms of
cell viability, cell morphology and biomarkers of macrophage polarization. Non-
treated AM porous titanium triggered a strong pro-inflammatory response in
macrophages, albeit combined with signs of anti-inflammatory effects. The PEO
treatment of AM porous titanium implants showed a higher potential to induce
polarization towards a pro-repair macrophage phenotype. We detected no cytotoxicity
against hMSCs in any of the groups. However, the incorporation of silver
nanoparticles resulted in strong cytotoxicity against attached macrophages. The
results of this study indicate the potential immunomodulatory effects of the AM
porous titanium enhanced with PEO treatment, and point towards caution and further
research when using silver nanoparticles for preventing IAIs. © 2020 The Author(s).
Published by IOP Publishing Ltd.
AU - Razzi, F.
AU - Fratila-Apachitei, L. E.
AU - Fahy, N.
AU - Bastiaansen-Jenniskens, Y. M.
AU - Apachitei, I.
AU - Farrell, E.
AU - Zadpoor, A. A.
C7 - 035017
DB - Scopus
DO - 10.1088/1748-605X/ab7763
IS - 3
KW - Alloys
Biomarkers
Bone Regeneration
Cell Survival
Cells, Cultured
Humans
Immunomodulation
Inflammation
Ions
Lasers
Macrophages
Materials Testing
Metal Nanoparticles
Monocytes
Nanoparticles
Phenotype
Porosity
Printing, Three-Dimensional
Silver
Stress, Mechanical
Surface Properties
Titanium
Aluminum alloys
Electrolysis
Metal implants
Metal nanoparticles
Polarization
Selective laser melting
Titanium alloys
biological marker
biomaterial
C-C motif chemokine 18
CD163 antigen
interleukin 1beta
interleukin 6
silver nanoparticle
titanium
vasculotropin
alloy
ion
macrogol
metal nanoparticle
nanoparticle
silver
titanium alloy (TiAl6V4)
Immunomodulatory effects
Mesenchymal stromal cells
Metallic biomaterials
Plasma electrolytic oxidation
Porous titanium implants
Surface biofunctionalization
Article
cell polarity
cell structure
cell viability
controlled study
cytotoxicity
electrolysis
human
human cell
immune response
immunomodulation
inflammation
macrophage
mesenchymal stroma cell
oxidation
phenotype
porosity
selective laser melting
surface property
bone regeneration
cell culture
cell survival
chemistry
cytology
drug effect
laser
materials testing
mechanical stress
metabolism
monocyte
mononuclear cell
Morphology
M3 - Article
PY - 2020
ST - Immunomodulation of surface biofunctionalized 3D printed porous titanium
implants
TI - Immunomodulation of surface biofunctionalized 3D printed porous titanium
implants
85084167134&doi=10.1088%2f1748-605X
%2fab7763&partnerID=40&md5=610c7abb85f88e58c5e97d85bff06666
VL - 15
ID - 5323
ER -
TY - JOUR
AB - The potential human health risks following the exposure to inorganic
nanoparticles (NPs) is a very important issue for their application in leather
finishing industry. The aim of our study was to investigate the cytotoxic effect of
silver (Ag)/titanium dioxide (TiO 2 ) NPs on human cells. Photocatalytic NPs were
prepared by electrochemical deposition of Ag on the surface of TiO 2 and nitrogen
(N)-TiO 2 NPs and, subsequently, physico-chemical characterized. Then, a set of
experiments have been performed to study the cytotoxicity and cell death mechanisms
involved, the changes in cell morphology and the production of ROS induced in human
keratinocytes (HaCaT) and human lung epithelial cells (A549) by exposure to NPs.
Moreover, the changes in major signaling pathways and the inflammatory response
induced by Ag/N-TiO 2 NPs in A549 cells were investigated. The data showed that
cell death by late apoptosis/necrosis is induced in cells as function of the dose
and the type of NPs and is characterized by morphological changes and cytoskeletal
disorganization and an increase in reactive oxygen species (ROS) production. The
exposure of A549 cells to Ag/N-TiO 2 NPs determine the activation of ERK1/2 MAP-
kinase pathway and the release of pro-inflammatory mediators CXCL1, GM-CSF and MIF,
known to be involved in the recruitment of circulating neutrophils and monocytes. ©
2019 Elsevier B.V.
AU - Rebleanu, D.
AU - Gaidau, C.
AU - Voicu, G.
AU - Constantinescu, C. A.
AU - Mansilla Sánchez, C.
AU - Rojas, T. C.
AU - Carvalho, S.
AU - Calin, M.
DB - Scopus
DO - 10.1016/j.tox.2019.01.013
KW - Ag/N-TiO 2
Ag/TiO 2
Cytokines
Cytotoxicity
Intracellular signalling
Nanoparticles
A549 Cells
Apoptosis
Catalysis
Extracellular Signal-Regulated MAP Kinases
Humans
Keratinocytes
Metal Nanoparticles
Necrosis
Oxidative Stress
Photochemical Processes
Respiratory Mucosa
Risk Assessment
Signal Transduction
Silver Compounds
Titanium
nitrogen
silver nanoparticle
autacoid
cytokine
metal nanoparticle
silver derivative
titanium
titanium dioxide
Article
cell culture
cell death
cell structure
cytoskeleton
cytotoxicity
human
human cell
hydrodynamics
keratinocyte
lung alveolus cell
lung epithelium
monocyte
neutrophil
photocatalysis
physical constitution and health
priority journal
signal transduction
surface property
zeta potential
A-549 cell line
apoptosis
catalysis
comparative study
drug effect
metabolism
necrosis
oxidative stress
pathology
photochemistry
respiratory mucosa
risk assessment
M3 - Article
PY - 2019
SP - 30-43
ST - The impact of photocatalytic Ag/TiO 2 and Ag/N-TiO 2 nanoparticles on human
keratinocytes and epithelial lung cells
T2 - Toxicology
TI - The impact of photocatalytic Ag/TiO 2 and Ag/N-TiO 2 nanoparticles on human
85061342839&doi=10.1016%2fj.tox.2019.01.013&partnerID=40&md5=111542f96ecbca65b0bac0
d033aa02c4
VL - 416
ID - 5368
ER -
TY - JOUR
AB - The potential human health risks following the exposure to inorganic
nanoparticles (NPs) is a very important issue for their application in leather
finishing industry. The aim of our study was to investigate the cytotoxic effect of
silver (Ag)/titanium dioxide (TiO2) NPs on human cells. Photocatalytic NPs were
prepared by electrochemical deposition of Ag on the surface of TiO2 and nitrogen
(N)-TiO2 NPs and, subsequently, physicochemical characterized. Then, a set of
experiments have been performed to study the cytotoxicity and cell death mechanisms
involved, the changes in cell morphology and the production of ROS induced in human
keratinocytes (HaCaT) and human lung epithelial cells (A549) by exposure to NPs.
Moreover, the changes in major signaling pathways and the inflammatory response
induced by Ag/N-TiO2 NPs in A549 cells were investigated. The data showed that cell
death by late apoptosis/necrosis is induced in cells as function of the dose and
the type of NPs and is characterized by morphological changes and cytoskeletal
disorganization and an increase in reactive oxygen species (ROS) production. The
exposure of A549 cells to Ag/N-TiO2 NPs determine the activation of ERK1/2 MAP-
kinase pathway and the release of pro-inflammatory mediators CXCL1, GM-CSF and MIF,
known to be involved in the recruitment of circulating neutrophils and monocytes.
AN - WOS:000461262900004
AU - Rebleanu, D.
AU - Gaidau, C.
AU - Voicu, G.
AU - Constantinescu, C. A.
AU - Sanchez, C. M.
AU - Rojas, T. C.
AU - Carvalho, S.
AU - Calin, M.
DA - MAR 15
DO - 10.1016/j.tox.2019.01.013
PY - 2019
SN - 0300-483X
SP - 30-43
ST - The impact of photocatalytic Ag/TiO2 and Ag/N-TiO2 nanoparticles on human
T2 - TOXICOLOGY
TI - The impact of photocatalytic Ag/TiO2 and Ag/N-TiO2 nanoparticles on human
VL - 416
ID - 6384
ER -
TY - JOUR
AB - The clinical features Of the maternal syndrome of pre-eclampsia can be
explained by generalised maternal endothelial cell dysfunction, which is a part of
a more global maternal systemic inflammatory response. There is growing evidence
that these effects are associated with the shedding of cellular debris, including
syncytiotrophoblast microparticles (STBM), cell-free DNA and mRNA, from the surface
of the placenta (syncytiotrophoblast) into the maternal circulation. The increased
shedding of this debris seen in pre-eclampsia is believed to be caused by placental
ischaemia, reperfusion and oxidative stress. This, labour and subsequent placental
study was carried out to determine whether uterine contractions during labour and
subsequent placental separation lead to an acute increase in the increase of
placental debt is into the maternal circulation. To assess the effects of labour,
samples were tal(en from 10 normal pregnant (NP) and 10 pre-eclamptic (PF) women at
varied time points. Similarly to assess the effects of placental delivery, plasma
samples were taken From 10 Nil and 10 I'll women Undergoing elective caesarean
section. There was a significant increase in the shedding of STBM in pre-eclampsia
which was not seen in normal pregnancy and there was a small rise in STBM levels at
placental separation in both normal pregnant and pre-eclamptic women undergoing
caesarean section, but the differences were not significant. However, levels of
placental cell-free corticotrophin releasing hormone mRNA were significantly
increased ill labour in both normal pregnancy and pre-eclampsia and were still high
24 h after delivery in the pre-eclamptic women. There was no significant increase
in fetal or total DNA in labour, but the overall levels of total DNA (maternal and
fetal) was increased ill labour In pre-eclampsia compared to normal labour. The
enhanced shedding of STBM and CRH mRNA in pre-eclampsia labour may have a role in
cases of postpartum worsening of pre-eclampsia. (C) 2008 Elsevier Ltd. All rights
reserved.
AN - WOS:000261089000005
AU - Reddy, A.
AU - Zhong, X. Y.
AU - Rusterholz, C.
AU - Hahn, S.
AU - Holzgreve, W.
AU - Redman, C. W. G.
AU - Sargent, I. L.
DA - NOV
DO - 10.1016/j.placenta.2008.08.018
IS - 11
PY - 2008
SN - 0143-4004
1532-3102
SP - 942-949
ST - The Effect of Labour and Placental Separation on the Shedding of
Syncytiotrophoblast Microparticles, Cell-free DNA and mRNA in Normal Pregnancy and
Pre-eclampsia
T2 - PLACENTA
TI - The Effect of Labour and Placental Separation on the Shedding of
Syncytiotrophoblast Microparticles, Cell-free DNA and mRNA in Normal Pregnancy and
Pre-eclampsia
VL - 29
ID - 6589
ER -
TY - JOUR
AB - Production of nanoscale materials often requires the use of toxic chemicals
and complex synthetic procedures. A new scaffold has been explored for in situ
synthesis of nanomaterials that utilizes natural biological systems in the form of
plants, bacteria, fungi, algae and redox-imbalanced mammalian cells and systems.
The latter approach has become popular in recent years and has shown some promising
results in bioimaging of cancer, as well as inflammatory and neurodegenerative
maladies. Biosynthesis of nanoclusters in redox-imbalanced mammalian cells is
facile, cost-effective and environmentally friendly with higher biocompatibility
and target specificity and lower adverse effects than traditional synthetic
approaches. Herein, we describe recent advances in mammalian green in situ
biosynthesis for biomedical applications, especially in cancer and
neurodegenerative disease theranostics.
AN - WOS:000448340500001
AU - Rehman, F. U.
AU - Jiang, H.
AU - Selke, M.
AU - Wang, X. M.
DA - NOV 7
DO - 10.1039/c8tb01955j
IS - 41
PY - 2018
SN - 2050-750X
2050-7518
SP - 6501-6514
ST - Mammalian cells: a unique scaffold for in situ biosynthesis of metallic
nanomaterials and biomedical applications
TI - Mammalian cells: a unique scaffold for in situ biosynthesis of metallic
nanomaterials and biomedical applications
VL - 6
ID - 6564
ER -
TY - JOUR
AB - Cardiac mast cells proliferate in cardiovascular diseases. In myocardial
ischemia, mast cell mediators contribute to coronary vasoconstriction, arrhythmias,
leukocyte recruitment, and tissue injury and repair. Arrhythmic dysfunction,
coronary vasoconstriction, and contractile failure are also characteristic of
cardiac anaphylaxis. In coronary atherosclerosis, mast cell mediators facilitate
cholesterol accumulation and plaque destabilization. In cardiac failure, mast cell
chymase causes myocyte apoptosis and fibroblast proliferation, leading to
ventricular dysfunction. Chymase and tryptase also contribute to fibrosis in
cardiomyopathies and myocarditis. In addition, mast cell tumor necrosis factor-
alpha promotes myocardial remodeling. Cardiac remodeling and hypertrophy in end-
stage hypertension are also induced by mast cell mediators and proteases. We
recently discovered that cardiac mast cells contain and release renin, which
initiates local angiotensin formation. Angiotensin causes coronary
vasoconstriction, arrhythmias, fibrosis, apoptosis, and endothelin release, all
demonstrated mechanisms of mast-cell-associated cardiac disease. The effects of
angiotensin are further amplified by the release of norepinephrine from cardiac
sympathetic nerves. Our discovery of renin in cardiac mast cells and its release in
pathophysiological conditions uncovers an important new pathway in the development
of mast-cell-associated heart diseases. Several steps in this novel pathway may
constitute future therapeutic targets.
AN - WOS:000246317100010
AU - Reid, A. C.
AU - Silver, R. B.
AU - Levi, R.
DA - JUN
DO - 10.1111/j.1600-065X.2007.00514.x
PY - 2007
SN - 0105-2896
1600-065X
SP - 123-140
ST - Renin: at the heart of the mast cell
T2 - IMMUNOLOGICAL REVIEWS
TI - Renin: at the heart of the mast cell
VL - 217
ID - 6493
ER -
TY - JOUR
AB - Background: It is well known that most neurodegenerative diseases are
associated with microglia-mediated inflammation. Our previous research demonstrates
that the CD40 signaling is critically involved in microglia-related immune
responses in the brain. For example, it is well known that the activation of the
signal transducer and activator of transcription (STAT) signaling pathway plays a
central role in interferon-gamma (IFN-gamma)-induced microglial CD40 expression. We
and others have previously reported that microglial CD40 expression is
significantly induced by IFN-gamma and amyloid-beta (A beta) peptide. Recent
studies have shown that certain flavonoids possess antiinflammatory and
neuroprotective properties distinct from their well-known anti-oxidant effects. In
particular, flavonoids, apigenin and luteolin have been found to be effective CD40
immunomodulators. Methods: Cultured microglia, both N9 and primary derived lines,
were treated with flavonoids in the presence of IFN-gamma and/or CD40 ligation to
assess any anti-inflammatory effects and/or mechanisms. CD40 expression on
microglia was analyzed by fluorescence activated cell sorting (FACS). Anti-
inflammatory effects and mechanisms were confirmed by ELISA for interlekin-6 (IL-6)
and TNF-alpha, lactate dehydrogenase (LDH) assay, and STAT1 Western blotting.
Results: Apigenin and luteolin concentration-dependently suppressed IFN-gamma-
induced CD40 expression. Apigenin and luteolin also suppressed microglial TNF-alpha
and IL-6 production stimulated by IFN-gamma challenge in the presence of CD40
ligation. In addition, apigenin and luteolin markedly inhibited IFN-gamma- induced
phosphorylation of STAT1 with little impact on cell survival. Conclusion: Our
findings provide further support for apigenin and luteolin's anti-inflammatory
effects and suggest that these flavonoids may have neuroprotective/disease-
modifying properties in various neurodegenerative disorders, including Alzheimer's
disease (AD).
AN - WOS:000260338200001
AU - Rezai-Zadeh, K.
AU - Ehrhart, J.
AU - Bai, Y.
AU - Sanberg, P. R.
AU - Bickford, P.
AU - Tan, J.
AU - Shytle, R. D.
C7 - 41
DA - SEP 25
DO - 10.1186/1742-2094-5-41
PY - 2008
SN - 1742-2094
ST - Apigenin and luteolin modulate microglial activation via inhibition of STAT1-
induced CD40 expression
T2 - JOURNAL OF NEUROINFLAMMATION
TI - Apigenin and luteolin modulate microglial activation via inhibition of STAT1-
induced CD40 expression
VL - 5
ID - 6630
ER -
TY - JOUR
AB - Objectives: Infliximab, an anti-tumor necrosis factor (TNF) monoclonal
antibody, might exert some of its long-term therapeutic effects in Crohn's disease
(CD) by interacting directly with cells of the immune system such as monocytes and
T lymphocytes via membrane TNF and by inducing apoptosis. Accordingly, the effects
of infliximab on monocyte apoptosis and down-regulation of proinflammatory
cytokines (reverse signaling) were assessed. Methods: To assess apoptosis,
monocytes from healthy individuals (controls) and CD patients were incubated in the
presence or absence of infliximab or the apoptotic agent gliotoxin for 24 hours.
Annexin V staining and the terminal deoxynucleotidyl transferase (TdT)-mediated
dUTP-FITC nick end labeling assay were used to measure early and late apoptosis. To
measure the effects of infliximab on reverse signaling, monocytes from healthy
individuals pretreated in vitro with infliximab were stimulated with
lipopolysaccharide or staphylococcal enterotoxin A, and the induction of the
proinflammatory cytokines, TNF-alpha, interleukin (IL)-1beta, IL-6, and IL-8 was
measured by reverse transcription polymerase chain reaction. The effect of in vivo
infliximab treatment of monocytes was similarly determined by comparing the
responses of monocytes from CD patients before and immediately after infliximab
infusion. Results: Infliximab did not induce apoptosis of monocytes from either
healthy individuals or CD patients but rather stabilized them. However, monocytes
from healthy individuals treated with infliximab in vitro, or from CD patients
infused with infliximab, produced significantly less TNF and other proinflammatory
cytokines when stimulated with the bacterial products lipopolysaccharide and
staphylococcal enterotoxin A. Conclusions: Apoptosis of monocytes is not
responsible for the therapeutic effects of infliximab. However, some of the
therapeutic effects of infliximab may be caused by its ability to down-regulate
proinflammatory cytokines production by monocytes exposed to bacterial antigens.
AN - WOS:000224909600015
AU - Ringheanu, M.
AU - Daum, F.
AU - Markowitz, J.
AU - Levine, J.
AU - Katz, S.
AU - Lin, X. Y.
AU - Silver, J.
DA - NOV
DO - 10.1097/00054725-200411000-00015
IS - 6
PY - 2004
SN - 1078-0998
1536-4844
SP - 801-810
ST - Effects of infliximab on apoptosis and reverse signaling of monocytes from
healthy individuals and patients with Crohn's disease
T2 - INFLAMMATORY BOWEL DISEASES
TI - Effects of infliximab on apoptosis and reverse signaling of monocytes from
healthy individuals and patients with Crohn's disease
VL - 10
ID - 6471
ER -
TY - JOUR
AB - Although environmental airborne silver nanoparticles (AgNPs) levels in
occupational and environmental settings are harmful to humans, the precise toxic
effects at the portal entry of exposure and after translocation to distant organs
are still to be deeply clarified. To this aim, the present study assessed
histopathological and ultrastructural alterations (by means of H&E and TEM,
respectively) in rat lung and liver, 7 and 28 days after a single intratracheal
instillation (i.t) of a low AgNP dose (50 microg/rat), compared to those induced by
an equivalent dose of ionic silver (7 microg AgNO3/rat). Lung parenchyma injury was
observed acutely after either AgNPs or AgNO3, with the latter compound causing more
pronounced effects. Specifically, alveolar collapse accompanied by inflammatory
alterations and parenchymal fibrosis were revealed. These effects lasted until the
28th day, a partial pulmonary structure recovery occurred, nevertheless a
persistence of slight inflammatory/fibrotic response and apoptotic phenomena were
still detected after AgNPs and AgNO3, respectively. Concerning the liver, a diffuse
hepatocyte injury was observed, characterized by cytoplasmic damage and dilation of
sinusoids, engulfed by degraded material, paralleled by inflammation onset. These
effects already detectable at day 7, persisting at the 28th day with some
attenuations, were more marked after AgNO3 compared to AgNPs, with the latter able
to induce a ductular reaction. Altogether the present findings indicate toxic
effects induced by AgNPs both at the portal entry (i.e. lung) and distant tissue
(i.e. liver), although the overall pulmonary damage were more striking compared to
the hepatic outcomes. © 2019 The Authors
AU - Roda, E.
AU - Bottone, M. G.
AU - Biggiogera, M.
AU - Milanesi, G.
AU - Coccini, T.
DB - Scopus
DO - 10.1016/j.toxrep.2019.09.008
KW - Health safety
In vivo toxicity
Nanoparticles
Occupational & environmental exposure
Risk assessment
Translocation
silver nanoparticle
animal cell
animal experiment
animal model
animal tissue
Article
atelectasis
controlled study
histology
histopathology
intratracheal drug administration
liver cell damage
lung fibrosis
lung injury
lung parenchyma
lung structure
male
nonhuman
priority journal
rat
M3 - Article
PY - 2019
SP - 1047-1060
ST - Pulmonary and hepatic effects after low dose exposure to nanosilver: Early
and long-lasting histological and ultrastructural alterations in rat
T2 - Toxicology Reports
TI - Pulmonary and hepatic effects after low dose exposure to nanosilver: Early
and long-lasting histological and ultrastructural alterations in rat
85073101327&doi=10.1016%2fj.toxrep.2019.09.008&partnerID=40&md5=4580f86b603264e95d6
881317895bf87
VL - 6
ID - 5438
ER -
TY - JOUR
AB - In our current study, we synthesized silver nanoparticles (AgNPs) from an
aqueous seed extract of Nigella sativa. The seed extract contains phytochemical
compounds including phenols, terpenoids, and flavonoids that may act as reducing
agents and are able to convert metal ions to metal nanoparticles. The formation of
synthesized AgNPs was characterized using UV-visible spectroscopy, Fourier
transform infra-red spectroscopy (FT-IR), scanning electron microscopy (SEM) and
energy dispersive analysis of X-rays (EDX). The efficacy of N-AgNPs against human
breast cancer (MCF-7) cells was tested. The synthesized AgNPs displayed dose-
dependent cytotoxicity (1–200 µg/mL) against MCF-7 cells. Morphological alterations
of the cells also appeared as bright field images. Treatment of synthesized AgNPs
altered the expression of Bax and Bcl-2 (apoptotic proteins) and COX-2
(inflammatory marker) in MCF-7 cells. To our knowledge, this is the first report
demonstrating that N-AgNPs from Nigella sativa can induce apoptosis in MCF-7 cells.
© 2019 by Begell House, Inc.
AU - Rohini, B.
AU - Akther, T.
AU - Waseem, M.
AU - Khan, J.
AU - Kashif, M.
AU - Hemalatha, S.
DB - Scopus
DO - 10.1615/JEnvironPatholToxicolOncol.2019027318
IS - 2
KW - AgNPs
Apoptosis
Breast cancer
Mitochondria
Breast Neoplasms
Humans
MCF-7 Cells
Metal Nanoparticles
Nigella sativa
Plant Extracts
Seeds
Silver
cyclooxygenase 2
plant extract
protein Bax
protein bcl 2
silver nanoparticle
metal nanoparticle
silver
Article
black cumin
breast cancer
drug cytotoxicity
human
human cell
in vitro study
MCF-7 cell line
priority journal
apoptosis
breast tumor
chemistry
drug effect
plant seed
M3 - Article
PY - 2019
SP - 185-194
ST - AgNPs from nigella sativa control breast cancer: An in vitro study
T2 - Journal of Environmental Pathology, Toxicology and Oncology
TI - AgNPs from nigella sativa control breast cancer: An in vitro study
85069809028&doi=10.1615%2fJEnvironPatholToxicolOncol.2019027318&partnerID=40&md5=55
4cd86d970d1e1fd76324c911ef8685
VL - 38
ID - 5456
ER -
TY - JOUR
AB - Despite the fact that the association of Helicobacter pylori with an
increased risk of gastric cancer is well documented, the exact mechanisms of this
association have not been elucidated. Our aim was to shed some light on these
mechanisms by studying the relationship of H. pylori CagA status to gastric cell
proliferation and apoptosis, since both play an important role in gastrointestinal
epithelial cell turnover and carcinogenesis. We studied fifty patients [32 men, 18
women, median age 39.5 years (range 18-67)], referred for upper gastrointestinal
endoscopy, from whom antral biopsies were taken. On biopsy specimens gastritis was
estimated by scoring the severity of inflammatory infiltrate, and the presence of
atrophy and intestinal metaplasia were also noted. The gastric cell proliferation
index (PI) was estimated by AgNOR staining, the epithelial apoptotic index (AI) was
measured by special staining for apoptosis, and CagA status was determined
serologically by immunoblotting the sera of patients against H. pylori antigens.
Thirty-eight (76%) of the 50 patients were H. pylori (positive) and 12 (24%) H.
pylori (negative). Among the 38 H. pylori(+) patients, 28 (73.6%) were CagA(+) and
10 (24.6%) CagA(-). In the H. pylori CagA(+) and CagA(-) groups, the PI values
[median (ranges)] were 5 (4-7) and 3.7 (3.5-5.5), respectively (P < 0.05). In
addition the difference in PI between the H. pylori CagA(+) and H. pylori(-) groups
was highly significant (P < 0.001). Concerning apoptosis, in the H. pylori CagA(+)
and CagA(-) groups, the values for AI were 1 (1-30) and 5.5 (1-35), respectively (P
< 0.05). In addition, the difference in AI between the H. pylori CagA(-) and H.
pylori(-) groups, was significant (P < 0.05). We conclude that H. pylori CagA(+)
strains induce increased gastric cell proliferation, which is not accompanied by a
parallel increase in apoptosis. This might explain the increased risk for gastric
carcinoma that is associated with infection by H. pylori CagA(+) strains.
AU - Rokkas, T.
AU - Ladas, S.
AU - Liatsos, C.
AU - Petridou, E.
AU - Papatheodorou, G.
AU - Theocharis, S.
AU - Karameris, A.
AU - Raptis, S.
DB - Scopus
DO - 10.1023/A:1026636803101
IS - 3
KW - Apoptosis
CagA Status
Gastric carcinogenesis
Helicobacter pylori infection
Proliferation
adult
aged
apoptosis
article
cancer risk
cell proliferation
clinical article
female
gastritis
Helicobacter pylori
human
male
priority journal
silver staining
stomach biopsy
stomach cancer
stomach carcinogenesis
M3 - Article
PY - 1999
SP - 487-493
ST - Relationship of Helicobacter pylori CagA status to gastric cell proliferation
and apoptosis
T2 - Digestive Diseases and Sciences
TI - Relationship of Helicobacter pylori CagA status to gastric cell proliferation
and apoptosis
0032987485&doi=10.1023%2fA
%3a1026636803101&partnerID=40&md5=0e8fde5130c2d7c686dead8dba4e58a3
VL - 44
ID - 5802
ER -
TY - JOUR
AB - There are many efforts in understanding the effects of nanoparticles on cell
viability and metabolism, however, not much is known regarding the distinct
molecular mechanisms of inflammation and cellular stress using low dosing
concentrations. To address this gap in the literature, we utilized a novel
experimental design that specifically probes the effects of a panel of commonly
studied engineered nanomaterials along immunomodulatory pathways, including NF-
kappa B. The panel of particles selected for this study included quantum dot
nanocrystals, titanium dioxide, hydroxylated fullerenes, and silver nanoparticles.
Cell viability, antioxidant activity, select messenger RNA, and protein modulation
were studied in primary human dermal fibroblasts (HDF) and NF-kappa B knockdown HDF
cells. Inflammatory and non-inflammatory immune responses were measured using
protein and real-time PCR array analysis from HDF cells exposed to sub-lethal
concentrations of nanoparticles. Differences in cellular response to nanoparticles
in protein and antioxidant experiments were evident in NF-kappa B knockdown cells.
The methods used in the study, along with the resultant data sets, serve as a
potential model for studying the complex pathway-specific biochemical responses in
cell and tissue systems associated with nanoparticle exposures. (C) 2012 Elsevier
Ireland Ltd. All rights reserved.
AN - WOS:000303623600004
AU - Romoser, A. A.
AU - Figueroa, D. E.
AU - Sooresh, A.
AU - Scribner, K.
AU - Chen, P. L.
AU - Porter, W.
AU - Criscitiello, M. F.
AU - Sayes, C. M.
DA - MAY 5
DO - 10.1016/j.toxlet.2012.01.022
IS - 3
PY - 2012
SN - 0378-4274
1879-3169
SP - 293-301
ST - Distinct immunomodulatory effects of a panel of nanomaterials in human dermal
fibroblasts
TI - Distinct immunomodulatory effects of a panel of nanomaterials in human dermal
fibroblasts
VL - 210
ID - 6408
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are useful to a wide range of consumer's and
medical products, due to their antimicrobial and anti-inflammatory activities.
AgNPs have been used to prevent the microbial colonization, therefore decreasing
the risk of infection, on implantable devices, tumor prostheses, bone cement and
surgical instruments. However, the putative toxicity of AgNPs to bone cells is
still poorly understood. Therefore, this study aimed to contribute to enlighten the
role of ionic silver release of small sized NPs on the biological outcomes of bone
cells, in particular to what concerns to induction of cytotoxic and genotoxic
effects. To achieve that goal osteoblast-like MG-63 cells were exposed to well
characterized PVP coated AgNPs of two different primary sizes (10 nm and 20 nm) and
evaluated after 24 and 48 h. Our results showed that, the smaller sized AgNPs
(10 nm) are more reactive and prone to form large aggregates, being therefore
mandatory to provide a careful characterization of the particles, before the
toxicity assessment. We also demonstrate that for short period exposures (up to
48 h) ionic silver (from AgNO3) is more toxic than the corresponding dose of AgNP.
However, when assessing longer term exposures by the clonogenic assay, we
demonstrated the inverse effect, the AgNPs turn out being more toxic, completely
inhibiting plate efficiency. Therefore, AgNPs toxicity cannot be attributed to the
dissociated Ag+ alone. Also, when comparing size-dependent effects, we demonstrate
that AgNP20 were found to induce a cell cycle arrest at G0/G1 and apoptosis, while
AgNP10 did not induce a cytostatic effect, but rather induced necrosis. Finally,
combining the chemical and toxicological profiles of both AgNP sizes, we
hypothesize that the size dependent AgNP toxicity may be associated in part with
the NPs interference with the cell membranes and consequent uptake/adsorption
processes. © 2016 Elsevier Ireland Ltd
AU - Rosário, F.
AU - Hoet, P.
AU - Santos, C.
AU - Oliveira, H.
DB - Scopus
DO - 10.1016/j.tox.2016.08.020
KW - Apoptosis
Cell cycle
Clonogenic assay
Necrosis
PVP-coating
Bone and Bones
Cell Cycle
Cell Cycle Checkpoints
Cell Line, Tumor
Cell Proliferation
Cell Survival
DNA Damage
Humans
Metal Nanoparticles
Osteoblasts
Particle Size
Silver
povidone
silver nanoparticle
metal nanoparticle
silver
apoptosis
Article
cell death
cell proliferation
cell viability
clonogenic assay
cytotoxicity
flow cytometry
genotoxicity
human
human cell
hydrodynamics
IC50
infection risk
micronucleus
molecular stability
osteoblast
osteosarcoma cell line
priority journal
bone
cell cycle
cell cycle checkpoint
cell survival
chemically induced
cytology
DNA damage
dose response
drug effects
necrosis
particle size
pathology
tumor cell line
M3 - Article
PY - 2016
SP - 103-115
ST - Death and cell cycle progression are differently conditioned by the AgNP size
in osteoblast-like cells
T2 - Toxicology
TI - Death and cell cycle progression are differently conditioned by the AgNP size
84987901663&doi=10.1016%2fj.tox.2016.08.020&partnerID=40&md5=61f48c615fbd137a0cc08f
fed8cfbfdf
VL - 368-369
ID - 5467
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are useful to a wide range of consumer's and
medical products, due to their antimicrobial and anti-inflammatory activities.
AgNPs have been used to prevent the microbial colonization, therefore decreasing
the risk of infection, on implantable devices, tumor prostheses, bone cement and
surgical instruments. However, the putative toxicity of AgNPs to bone cells is
still poorly understood. Therefore, this study aimed to contribute to enlighten the
role of ionic silver release of small sized NPs on the biological outcomes of bone
cells, in particular to what concerns to induction of cytotoxic and genotoxic
effects. To achieve that goal osteoblast-like MG-63 cells were exposed to well
characterized PVP coated AgNPs of two different primary sizes (10 nm and 20 nm) and
evaluated after 24 and 48 h. Our results showed that, the smaller sized AgNPs (10
nm) are more reactive and prone to form large aggregates, being therefore mandatory
to provide a careful characterization of the particles, before the toxicity
assessment. We also demonstrate that for short period exposures (up to 48 h) ionic
silver (from AgNO3) is more toxic than the corresponding dose of AgNP. However,
when assessing longer term exposures by the clonogenic assay, we demonstrated the
inverse effect, the AgNPs turn out being more toxic, completely inhibiting plate
efficiency. Therefore, AgNPs toxicity cannot be attributed to the dissociated Ag+
alone. Also, when comparing size-dependent effects, we demonstrate that AgNP20 were
found to induce a cell cycle arrest at G0/G1 and apoptosis, while AgNP10 did not
induce a cytostatic effect, but rather induced necrosis. Finally, combining the
chemical and toxicological profiles of both AgNP sizes, we hypothesize that the
size dependent AgNP toxicity may be associated in part with the NPs interference
with the cell membranes and consequent uptake/adsorption processes. (C) 2016
Elsevier Ireland Ltd. All rights reserved.
AN - WOS:000387199300011
AU - Rosario, F.
AU - Hoet, P.
AU - Santos, C.
AU - Oliveira, H.
DA - AUG 10
DO - 10.1016/j.tox.2016.08.020
PY - 2016
SN - 0300-483X
SP - 103-115
ST - Death and cell cycle progression are differently conditioned by the AgNP size
T2 - TOXICOLOGY
TI - Death and cell cycle progression are differently conditioned by the AgNP size
VL - 368
ID - 5978
ER -
TY - JOUR
AB - Glucagon Like Peptide-1 (GLP-1) drugs are currently used to treat type-2
diabetes. Safety concerns for increased risk of pancreatitis and pancreatic ductal
metaplasia have accompanied these drugs. High fat diet (HFD) is a type-2 diabetes
risk factor that may affect the response to GLP-1 drug treatment. The objective of
the present study was to investigate the effects of diet and GLP-1 based drugs on
the exocrine pancreas in mice. Experiments were designed in a mouse model of
insulin resistance created by feeding a HFD or standard diet (STD) for 6 weeks. The
GLP-1 drugs, sitagliptin (SIT) and exenatide (EXE) were administered once daily for
additional 6 weeks in both mice fed HFD or STD. The results showed that body
weight, blood glucose levels, and serum levels of pro-inflammatory cytokines (TNF
alpha, IL-1 beta, and KC) were significantly greater in HFD mice than in STD mice
regardless of GLP-1 drug treatment. The semi-quantitative grading showed that
pancreatic changes were significantly greater in EXE and SIT-treated mice compared
to control and that HFD exacerbated spontaneous exocrine pancreatic changes seen in
saline-treated mice on a standard diet. Exocrine pancreatic changes identified in
this study included acinar cell injury (hypertrophy, autophagy, apoptosis,
necrosis, and atrophy), vascular injury, interstitial edema and inflammation, fat
necrosis, and duct changes. These findings support HFD as a risk factor to
increased susceptibility/severity for acute pancreatitis and indicate that GLP-1
drugs cause pancreatic injury that can be exacerbated in a HFD environment.
Published by Elsevier Inc.
AN - WOS:000334821900003
AU - Rouse, R.
AU - Xu, L.
AU - Stewart, S.
AU - Zhang, J.
DA - APR 15
DO - 10.1016/j.taap.2014.01.021
IS - 2
PY - 2014
SN - 0041-008X
1096-0333
SP - 104-114
ST - High fat diet and GLP-1 drugs induce pancreatic injury in mice
TI - High fat diet and GLP-1 drugs induce pancreatic injury in mice
VL - 276
ID - 6681
ER -
TY - JOUR
AB - This study expanded upon a previous study in mice reporting a link between
exenatide treatment and exocrine pancreatic injury by demonstrating temporal and
dose responses and providing an initial mechanistic hypothesis. The design of the
present study included varying lengths of exenatide exposure (3, 6 weeks to 12
weeks) at multiple concentrations (3, 10, or 30 mu g/kg) with multiple endpoints
(histopathology evaluations, immunoassay for cytokines, immunostaining of the
pancreas, serum chemistries and measurement of trypsin, amylase, and, lipase, and
gene expression profiles). Time- and dose-dependent exocrine pancreatic injury was
observed in mice on a high fat diet treated with exenatide. The morphological
changes identified in the pancreas involved acinar cell injury and death
(autophagy, apoptosis, necrosis, and atrophy), cell adaptations (hypertrophy and
hyperplasia), and cell survival (proliferation/regeneration) accompanied by varying
degrees of inflammatory response leading to secondary injury in pancreatic blood
vessels, ducts, and adipose tissues. Gene expression profiles indicated increased
signaling for cell survival and altered lipid metabolism in exenatide treated mice.
Immunohistochemistry supported gene expression findings that exenatide caused
and/or exacerbated pancreatic injury in a high fat diet environment potentially by
further increasing high fat diet exacerbated lipid metabolism and resulting
oxidative stress. Further investigation is required to confirm these findings and
determine their relevance to human disease.
AN - WOS:000342798000064
AU - Rouse, R.
AU - Zhang, L. S.
AU - Shea, K.
AU - Zhou, H. F.
AU - Xu, L.
AU - Stewart, S.
AU - Rosenzweig, B.
AU - Zhang, J.
C7 - e109477
DA - OCT 7
DO - 10.1371/journal.pone.0109477
IS - 10
PY - 2014
SN - 1932-6203
ST - Extended Exenatide Administration Enhances Lipid Metabolism and Exacerbates
Pancreatic Injury in Mice on a High Fat, High Carbohydrate Diet
T2 - PLOS ONE
TI - Extended Exenatide Administration Enhances Lipid Metabolism and Exacerbates
Pancreatic Injury in Mice on a High Fat, High Carbohydrate Diet
VL - 9
ID - 6717
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) have been increasingly incorporated into food-
related and hygiene products for their unique antimicrobial and preservative
properties. The consequent oral exposure may then result in unpredicted harmful
effects in the gastrointestinal tract (GIT), which should be considered in the risk
assessment and risk management of these materials. In the present study, the toxic
effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-
relevant cells (Caco-2 cell line as a model of human intestinal cells, and
neutrophils as a model of the intestinal inflammatory response). This study also
evaluated the putative protective action of dietary flavonoids against such harmful
effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced
Caco-2 cell death by apoptosis with concomitant production of nitric oxide,
irrespective of the size. It was also observed that AgNP induced human neutrophil
oxidative burst. Interestingly, some flavonoids, namely quercetin and
quercetagetin, prevented the deleterious effects of AgNP in both cell types.
Overall, the data of the present study provide a first insight into the promising
protective role of flavonoids against the potentially toxic effects of AgNP at the
intestinal level. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Rufino, A. T.
AU - Ramalho, A.
AU - Sousa, A.
AU - de Oliveira, J. M. P. F.
AU - Freitas, P.
AU - Gonzalez Gómez, M. A.
AU - Piñeiro-Redondo, Y.
AU - Rivas, J.
AU - Carvalho, F.
AU - Fernandes, E.
AU - Freitas, M.
C7 - 6610
DB - Scopus
DO - 10.3390/molecules26216610
IS - 21
KW - Flavonoids
Inflammation
Intestinal cells
Nanoparticles
Neutrophils
Apoptosis
Caco-2 Cells
Humans
Intestinal Mucosa
Metal Nanoparticles
Particle Size
Protective Agents
Silver
flavonoid
metal nanoparticle
protective agent
silver
apoptosis
Caco-2 cell line
chemistry
drug effect
human
inflammation
intestine mucosa
metabolism
particle size
M3 - Article
PY - 2021
ST - Protective role of flavonoids against intestinal pro-inflammatory effects of
T2 - Molecules
TI - Protective role of flavonoids against intestinal pro-inflammatory effects of
85118407693&doi=10.3390%2fmolecules26216610&partnerID=40&md5=8e93908e3d03968ee3ad86
70c1300c4c
VL - 26
ID - 5194
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) have been increasingly incorporated into food-
related and hygiene products for their unique antimicrobial and preservative
properties. The consequent oral exposure may then result in unpredicted harmful
effects in the gastrointestinal tract (GIT), which should be considered in the risk
assessment and risk management of these materials. In the present study, the toxic
effects of polyethyleneimine (PEI)-coated AgNP (4 and 19 nm) were evaluated in GIT-
relevant cells (Caco-2 cell line as a model of human intestinal cells, and
neutrophils as a model of the intestinal inflammatory response). This study also
evaluated the putative protective action of dietary flavonoids against such harmful
effects. The obtained results showed that AgNP of 4 and 19 nm effectively induced
Caco-2 cell death by apoptosis with concomitant production of nitric oxide,
irrespective of the size. It was also observed that AgNP induced human neutrophil
oxidative burst. Interestingly, some flavonoids, namely quercetin and
quercetagetin, prevented the deleterious effects of AgNP in both cell types.
Overall, the data of the present study provide a first insight into the promising
protective role of flavonoids against the potentially toxic effects of AgNP at the
intestinal level.
AN - WOS:000718640500001
AU - Rufino, A. T.
AU - Ramalho, A.
AU - Sousa, A.
AU - de Oliveira, Jmpf
AU - Freitas, P.
AU - Gomez, M. A. G.
AU - Pineiro-Redondo, Y.
AU - Rivas, J.
AU - Carvalho, F.
AU - Fernandes, E.
AU - Freitas, M.
C7 - 6610
DA - NOV
DO - 10.3390/molecules26216610
IS - 21
PY - 2021
SN - 1420-3049
ST - Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of
T2 - MOLECULES
TI - Protective Role of Flavonoids against Intestinal Pro-Inflammatory Effects of
VL - 26
ID - 5994
ER -
TY - JOUR
AB - Biodegradable polymers of natural origin are ideal for the development of
processes in tissue engineering due to their immunogenic potential and ability to
interact with living tissues. However, some synthetic polymers have been developed
in recent years for use in tissue engineering, such as Poly-epsilon-caprolactone.
The nanotechnology and the electrospinning process are perceived to produce
biomaterials in the form of nanofibers with diverse unique properties.
Biocompatibility tests of poly-epsilon-caprolactone nanofibers embedded with
hydroxyapatite and alumina nanoparticles manufactured by means of the
electrospinning technique were carried out in Wistar rats to be used as oral
dressings. Hydroxyapatite as a material is used because of its great compatibility,
bioactivity, and osteoconductive properties. The PCL, PCL-HA, PCL-alpha-Al2O3, and
PCL-HA-alpha-Al2O3 nanofibers obtained in the process were characterized by
infrared spectroscopy and scanning electron microscopy. The nanofibers had an
average diameter of (840 +/- 230) nm. The nanofiber implants were placed and tested
at 2, 4, and 6 weeks in the subcutaneous tissue of the rats to give a chronic
inflammatory infiltrate, characteristic foreign body reaction, which decreased
slightly at 6 weeks with the addition of hydroxyapatite and alumina ceramic
particles. The biocompatibility test showed a foreign body reaction that produces a
layer of collagen and fibroblasts. Tissue loss and necrosis were not observed due
to the coating of the material, but a slight decrease in the inflammatory
infiltrate occurred in the last evaluation period, which is indicative of the
beginning of the acceptance of the tested materials by the organism.
AN - WOS:000808673900001
AU - Ruiz-Ramirez, L. R.
AU - Alvarez-Ortega, O.
AU - Espinosa-Cristobal, L. F.
AU - Farias-Mancilla, J. R.
AU - Martinez-Perez, C. A.
AU - Reyes-Lopez, S. Y.
C7 - 2130
DA - JUN
DO - 10.3390/polym14112130
IS - 11
PY - 2022
SN - 2073-4360
ST - Poly-epsilon-Caprolactone-Hydroxyapatite-Alumina (PCL-HA-alpha-Al2O3)
Electrospun Nanofibers in Wistar Rats
T2 - POLYMERS
TI - Poly-epsilon-Caprolactone-Hydroxyapatite-Alumina (PCL-HA-alpha-Al2O3)
Electrospun Nanofibers in Wistar Rats
VL - 14
ID - 6463
ER -
TY - JOUR
AB - Although antimicrobial nanosilver finds numerous applications in the health
and food industries, the in vivo toxicity of positively charged silver
nanoparticles (AgNPs+) and relevant controls are largely unexplored. This study
investigates the relationship between the biodistribution and toxicity of the well-
known cetyltrimethylammonium bromide (CTAB)-capped AgNPs+ in 6-weeks old female
Sprague-Dawley rats, at sublethal doses. Amounts comparative to those leaked from
food products or considered for animal feed were administered through daily water
intake, for an 18-day period: AgNPs+ (40 μg mL−1), Ag+ (40 μg mL−1), antimicrobial
CTAB+ (24 μg mL−1) and tap water. All exposures except for the water control had
adverse effects on the health and systemic functions of rats (e.g., lethargy,
hepatomegaly, splenomegaly, impediment of bone development, and/or heightened
immune response). Although the total Ag accumulation in tissues (1.4–1.6 μg of Ag/g
of liver, spleen, jejunum, and brain) was comparable for the two Ag species, AgNPs+
were generally more toxic than Ag+, particularly in spleen (0.8 μg Ag/g).
Significantly reduced euthanasia time, alopecia, inflammatory responses in spleen,
fragile veins, and enhanced lymphocytosis were observed only for AgNPs+. Overall,
this study raises health concerns about the ingestion of capped-AgNPs+ or Ag+ by
first-hand consumers and industry workers. © 2022 Elsevier Ltd
AU - Ryan, J.
AU - Jacob, P.
AU - Lee, A.
AU - Gagnon, Z.
AU - Pavel, I. E.
C7 - 113228
DB - Scopus
DO - 10.1016/j.fct.2022.113228
KW - Atomic absorption spectroscopy
Histopathology
Raman spectroscopy
Rats
Silver ions
Animals
Cetrimonium
Female
Ions
Metal Nanoparticles
Silver
Tissue Distribution
Water
antiinfective agent
cetrimide
graphite
silver
silver nanoparticle
tap water
ion
metal nanoparticle
water
alopecia
animal cell
animal experiment
animal tissue
Article
blood examination
bone disease
bone tissue
brain disease
brain tissue
controlled study
euthanasia
female
fragile vein
graphite furnace atomic absorption spectrometry
hepatomegaly
histopathology
immunopathology
inflammation
ingestion
jejunum disease
jejunum tissue
lethargy
liver tissue
liver toxicity
lymphocytosis
nonhuman
Raman spectrometry
rat
spleen disease
spleen tissue
splenomegaly
Sprague Dawley rat
sublethal dose
tissue distribution
toxicity testing
vein disease
animal
M3 - Article
PY - 2022
ST - Biodistribution and toxicity of antimicrobial ionic silver (Ag+) and silver
nanoparticle (AgNP+) species after oral exposure, in Sprague-Dawley rats
TI - Biodistribution and toxicity of antimicrobial ionic silver (Ag+) and silver
nanoparticle (AgNP+) species after oral exposure, in Sprague-Dawley rats
85132841797&doi=10.1016%2fj.fct.2022.113228&partnerID=40&md5=59c24bd34f178937b63292
598bccb61c
VL - 166
ID - 5091
ER -
TY - JOUR
AB - Iron oxide nanoparticles (Fe3O4 NPs) are important for different medical
applications. However, potential toxicity has been reported and several parameters
must still be studied to reach highest therapeutic efficacy with minimal undesired
effects. Inflammation is one of the most reported undesired effects of NP exposure
in a variety of inflammatory models and conflicting data exist regarding whether
Fe3O4 NPs possess pro-or anti-inflammatory activities. The aim of this study was to
determine the direct effect of Fe3O4 NPs on the biology of neutrophil, a key player
cell in inflammation. Freshly isolated human neutrophils were incubated in vitro
with Fe3O4 NPs, and several functions have been studied. Using transmission
electronic microscopy, Fe3O4 NPs were found to be ingested by neutrophils. These
NPs do not induce a respiratory burst by themselves, but they increase the ability
of neutrophils to adhere onto human endothelial cells as well as enhance
phagocytosis. An antibody array approach revealed that Fe3O4 NPs induce the
production of some cytokines, including the chemokine IL-8 (CXCL8), which was
confirmed by ELISA. Fe(3)O(4)NPs were found to delay spontaneous neutrophil
apoptosis regardless of sex of the donor. Using a pharmacological approach, we
demonstrate that Fe3O4 NPs delay apoptosis by a de novo protein synthesis-dependent
mechanism and via different cell signalling pathways. The data indicate that Fe3O4
NPs can alter the biology of human neutrophils and that they possess some pro -
inflammatory effects, particularly based on their capacity to delay apoptosis and
to induce the production of pro-inflammatory cytokines. Therefore, Fe3O4 NPs can
regulate inflammation by targeting human neutrophil functions.
AN - WOS:000841136700001
AU - Saafane, A.
AU - Girard, D.
C6 - AUG 2022
C7 - 110053
DA - SEP 25
DO - 10.1016/j.cbi.2022.110053
PY - 2022
SN - 0009-2797
1872-7786
ST - Interaction between iron oxide nanoparticles (Fe3O4 NPs) and human
neutrophils: Evidence that Fe3O4 NPs possess some pro-inflammatory activities
TI - Interaction between iron oxide nanoparticles (Fe3O4 NPs) and human
neutrophils: Evidence that Fe3O4 NPs possess some pro-inflammatory activities
VL - 365
ID - 6295
ER -
TY - JOUR
AB - Nanotechnology has been gaining more and more momentum lately and the
potential use of nanomaterials such as nanoparticles (NPs) continues to grow in a
variety of activity sectors. Among the NPs, iron oxide nanoparticles (IONs) have
retained an increasing interest from the scientific community and industrials due
to their superparamagnetic properties allowing their use in many fields, including
medicine. However, some undesired effects of IONs and potential risk for human
health are becoming increasingly reported in several studies. Although many in vivo
studies reported that IONs induce immunotoxicity in different animal models, it is
not clear how IONs can alter the biology of primary human immune cells. In this
article, we will review the works that have been done regarding the interaction
between IONs and primary immune cells. This review also outlines the importance of
using primary immune cells in risk assessment of NPs as a reliable strategy for
encouraging non-animal studies approaches, to determine risks that might affect the
human immune system following different exposure scenarios. Taken all together, the
reported observations help to get a more global picture on how IONs alter the human
immune system especially the fact that inflammation, known to involve several
immune cell types, is frequently reported as an undesired effect of IONs. © 2023
AU - Saafane, A.
AU - Girard, D.
C7 - 105635
DB - Scopus
DO - 10.1016/j.tiv.2023.105635
KW - In vitro
Iron oxide nanoparticles
Nanotoxicology
Primary human immune cells
Humans
Inflammation
Ions
Magnetic Iron Oxide Nanoparticles
Nanoparticles
caspase 3
caspase 9
catalase
CD63 antigen
CD69 antigen
CD71 antigen
CXCL1 chemokine
ferritin
ferritin heavy chain
ferroportin
gamma interferon
glutathione
histone H3
interleukin 10
interleukin 12
interleukin 17
interleukin 1beta
interleukin 4
interleukin 5
interleukin 6
interleukin 8
leukocyte elastase
maghemite nanoparticle
natural cytotoxicity triggering receptor 2
natural cytotoxicity triggering receptor 3
natural killer cell receptor NKG2D
RANTES
reduced nicotinamide adenine dinucleotide phosphate oxidase
scavenger receptor A
silver nanoparticle
unclassified drug
ion
magnetic iron oxide nanoparticle
nanoparticle
apoptosis
B lymphocyte
basophil
cell migration
cell proliferation
cell protection
cell survival
cell viability
chemical interaction
cytokine production
cytokine release
cytotoxicity
degranulation
dendritic cell
DNA damage
endocytosis
eosinophil
genotoxicity
human
immortalized cell line
immune response
immunomodulation
inflammation
M1 macrophage
M2 macrophage
macropinocytosis
mast cell
monocyte
natural killer cell
NETosis
neutrophil
nuclear reprogramming
phagocytosis
protein expression
Review
T lymphocyte
M3 - Review
PY - 2023
ST - Interaction between iron oxide nanoparticles (IONs) and primary human immune
cells: An up-to-date review of the literature
TI - Interaction between iron oxide nanoparticles (IONs) and primary human immune
cells: An up-to-date review of the literature
85163173918&doi=10.1016%2fj.tiv.2023.105635&partnerID=40&md5=595753dbef8bcf450dc20a
13eaeda565
VL - 91
ID - 4991
ER -
TY - JOUR
AB - Background: Little is known of how the toxicity of nanoparticles is affected
by the incorporation in complex matrices. We compared the toxic effects of the
titanium dioxide nanoparticle UV-Titan L181 (NanoTiO(2)), pure or embedded in a
paint matrix. We also compared the effects of the same paint with and without
NanoTiO(2). Methods: Mice received a single intratracheal instillation of 18, 54
and 162 mu g of NanoTiO(2) or 54, 162 and 486 mu g of the sanding dust from paint
with and without NanoTiO(2). DNA damage in broncheoalveolar lavage cells and liver,
lung inflammation and liver histology were evaluated 1, 3 and 28 days after
intratracheal instillation. Printex 90 was included as positive control. Results:
There was no additive effect of adding NanoTiO(2) to paints: Therefore the toxicity
of NanoTiO(2) was reduced by inclusion into a paint matrix. NanoTiO(2) induced
inflammation in mice with severity similar to Printex 90. The inflammatory response
of NanoTiO(2) and Printex 90 correlated with the instilled surface area. None of
the materials, except of Printex 90, induced DNA damage in lung lining fluid cells.
The highest dose of NanoTiO(2) caused DNA damage in hepatic tissue 1 day after
intratracheal instillation. Exposure of mice to the dust from paints with and
without TiO2 was not associated with hepatic histopathological changes. Exposure to
NanoTiO(2) or to Printex 90 caused slight histopathological changes in the liver in
some of the mice at different time points. Conclusions: Pulmonary inflammation and
DNA damage and hepatic histopathology were not changed in mice instilled with
sanding dust from NanoTiO(2) paint compared to paint without NanoTiO(2). However,
pure NanoTiO(2) caused greater inflammation than NanoTiO(2) embedded in the paint
matrix.
AN - WOS:000301574900002
AU - Saber, A. T.
AU - Mortensen, A.
AU - Szarek, J.
AU - Jackson, P.
AU - Madsen, A. M.
AU - Jensen, K. A.
AU - Koponen, I. K.
AU - Brunborg, G.
AU - Gutzkow, K. B.
AU - Vogel, U.
AU - Wallin, H.
C7 - 4
DA - FEB 2
DO - 10.1186/1743-8977-9-4
PY - 2012
SN - 1743-8977
ST - Nanotitanium dioxide toxicity in mouse lung is reduced in sanding dust from
paint
TI - Nanotitanium dioxide toxicity in mouse lung is reduced in sanding dust from
paint
VL - 9
ID - 6494
ER -
TY - JOUR
AB - BackgroundThe development of nano delivery systems is rapidly emerging area
of nanotechnology applications where nanomaterials (NMs) are employed to deliver
therapeutic agents to specific site in a controlled manner. To accomplish this,
green synthesis of NMs is widely explored as an eco-friendly method for the
development of smart drug delivery system. In the recent times, use of green
synthesized NMs, especially metallic NMs have fascinated the scientific community
as they are excellent carriers for drugs. This work demonstrates optimized green,
biogenic synthesis of gold nanoparticles (AuNPs) for functionalization with
quercetin (QT) and camptothecin (CPT) to enhance potential anti-inflammatory, anti-
cancer and anti-angiogenic activities of these drugs.ResultsGold nanoparticles were
optimally synthesized in 8 min of reaction at 90 degrees C, pH 6, using 4 mM of
HAuCl4 and 4:1 ratio of extract: HAuCl4. Among different capping agents tested,
capping of AuNPs with polyethylene glycol 9000 (PG9) was found best suited prior to
functionalization. PG9 capped AuNPs were optimally functionalized with QT in 1 h
reaction at 70 degrees C, pH 7, using 1200 ppm of QT and 1:4 ratio of AuNPs-PG9:QT
whereas, CPT was best functionalized at RT in 1 h, pH 12, AuNPs-PG9:CPT ratio of
1:1, and 0.5 mM of CPT. QT functionalized AuNPs showed good anti-cancer activity
(IC50 687.44 mu g/mL) against MCF-7 cell line whereas test of anti-inflammatory
activity also showed excellent activity (IC50 287.177 mg/L). The CAM based
assessment of anti-angiogenic activity of CPT functionalized AuNPs demonstrated the
inhibition of blood vessel branching confirming the anti-angiogenic
effect.ConclusionsThus, present study demonstrates that optimally synthesized
biogenic AuNPs are best suited for the functionalization with drugs such as QT and
CPT. The functionalization of these drugs with biogenic AuNPs enhances the
potential anti-inflammatory, anti-cancer and anti-angiogenic activities of these
drugs, therefore can be used in biomedical application.
AN - WOS:000635198700002
AU - Sadalage, P. S.
AU - Patil, R. V.
AU - Havaldar, D. V.
AU - Gavade, S. S.
AU - Santos, A. C.
AU - Pawar, K. D.
C7 - 84
DA - MAR 25
DO - 10.1186/s12951-021-00836-1
IS - 1
PY - 2021
SN - 1477-3155
ST - Optimally biosynthesized, PEGylated gold nanoparticles functionalized with
quercetin and camptothecin enhance potential anti-inflammatory, anti-cancer and
anti-angiogenic activities
TI - Optimally biosynthesized, PEGylated gold nanoparticles functionalized with
quercetin and camptothecin enhance potential anti-inflammatory, anti-cancer and
anti-angiogenic activities
VL - 19
ID - 6119
ER -
TY - JOUR
AB - The use of natural polymers and electrospinning as a new method of wound
dressing production is one of the things that can revolutionize the medical world.
Due to the importance of wound healing and characteristics such as anti-
inflammatory and antimicrobial properties, it is possible to use natural compounds
such as fungi and metabolites derived from them to produce wound dressing. In this
study, schizophyllan (SPG) as an extracellular polysaccharide was extracted from
Iranian Schizophyllum commune (NCBI MG761830) and then the silver nanoparticles
(AgNPs) were produced by the in-situ method in 1.5 % SPG solution. Afterward, they
were combined with polyvinyl alcohol 10 % (PVA) polymer to strengthen the fiber
structure. We investigated the properties of nanofibers containing PVA/SPG-AgNPs
and PVA/SPG20 %. The physicochemical properties of two fibers were investigated by
SEM, TEM, FTIR, contact angle, water uptake, nanoparticle release, and biological
test (antibacterial, and MTT). The diameter of the nanofiber-containing the AgNPs
was about 169 nm and the other nanofiber was about 212 nm. The highest inhibition
of the growth of the bacterium by PVA/SPG-AgNPs against E. coli and S. aureus was
about 88.34 % and 64.7 %, respectively. The silver ion release from PVA/SPG-AgNPs
nanofibers was 21 μg/ml after fifth day. Both nanofibers had no toxic effect on
L929 fibroblast cells. © 2019, The Korean Fiber Society.
AU - Safaee-Ardakani, M. R.
AU - Hatamian-Zarmi, A.
AU - Sadat, S. M.
AU - Mokhtari-Hosseini, Z. B.
AU - Ebrahimi-Hosseinzadeh, B.
AU - Kooshki, H.
AU - Rashidiani, J.
DB - Scopus
DO - 10.1007/s12221-019-9388-8
IS - 12
KW - Cytotoxicity
In situ
Nanofiber
Schizophyllan
Silver nanoparticle
Cells
Contact Angle
Fibers
Natural Polymers
Polyvinyl Acetate
Release
Silver
Test Methods
Cell culture
Contact angle
Escherichia coli
Functional polymers
Metabolites
Metal ions
Metal nanoparticles
Natural polymers
Anti-inflammatory and antimicrobial properties
Extracellular polysaccharides
In-situ preparations
Natural compounds
Schizophyllum commune
Silver ion release
Nanofibers
M3 - Article
PY - 2019
SP - 2493-2502
ST - In situ Preparation of PVA/Schizophyllan-AgNPs Nanofiber as Potential of
Wound Healing: Characterization and Cytotoxicity
T2 - Fibers and Polymers
TI - In situ Preparation of PVA/Schizophyllan-AgNPs Nanofiber as Potential of
Wound Healing: Characterization and Cytotoxicity
85077066998&doi=10.1007%2fs12221-019-9388-
8&partnerID=40&md5=095e2a861a1027874fefd1db21a66b24
VL - 20
ID - 5378
ER -
TY - JOUR
AB - Nanoparticles have numerous biomedical applications including, but not
limited to, targeted drug delivery, diagnostic imaging, sensors, and implants for a
wide range of diseases including cancer, diabetes, heart disease, and tuberculosis.
Although the mode of delivery of the nanoparticles depends on the application and
the disease, the nanoparticles are often in immediate contact with the systemic
circulation either because of intravenous administration or their ability to enter
the bloodstream with relative ease or their longer survival time in circulation.
Once in circulation, the nanoparticles may elicit unintended hemostatic and
inflammatory responses, and hence the design of nanoparticles for therapeutic
applications should take broad hemocompatibility concerns into consideration. In
this review, we present the principles underlying the structural and functional
design of various classes of nanoparticles that are currently approved by the US
Food and Drug Administration, categorize these particles based on their
interactions with cardiovascular tissues and ensuing adverse events, and also
describe various in vitro assays that may be used evaluate their hemocompatibility.
AN - WOS:000544277900010
AU - Saha, A. K.
AU - Zhen, M. Y. S.
AU - Erogbogbo, F.
AU - Ramasubramanian, A. K.
DA - JUL
DO - 10.1055/s-0039-1688491
IS - 05
PY - 2020
SN - 0094-6176
1098-9064
SP - 637-651
ST - Design Considerations and Assays for Hemocompatibility of FDA-Approved
Nanoparticles
T2 - SEMINARS IN THROMBOSIS AND HEMOSTASIS
TI - Design Considerations and Assays for Hemocompatibility of FDA-Approved
Nanoparticles
VL - 46
ID - 6825
ER -
TY - JOUR
AB - Despite much recent progress, prostate cancer continues to represent a major
cause of cancer-related mortality and morbidity in men. Prostate cancer is the
mostcommonnonskin neoplasm and second leading cause of death in men. 6-Shogaol (6-
SHO), a potent bioactive compound in ginger (Zingiber officinale Roscoe), has been
shown to possess anti-inflammatory and anticancer activity. In the present study,
the effect of 6-SHO on the growth of prostate cancer cells was investigated. 6-SHO
effectively reduced survival and induced apoptosis of cultured human (LNCaP, DU145,
and PC3) and mouse (HMVP2) prostate cancer cells. Mechanistic studies revealed that
6-SHO reduced constitutive and interleukin (IL)-6-induced STAT3 activation and
inhibited both constitutive and TNF-alpha-induced NF-kB activity in these cells. In
addition, 6-SHO decreased the level of several STAT3 and NF-kB-regulated target
genes at the protein level, including cyclin D1, survivin, and cMyc and
modulatedmRNAlevels of chemokine, cytokine, cell cycle, and apoptosis regulatory
genes (IL-7, CCL5, BAX, BCL2, p21, and p27). 6-SHO was more effective than two
other compounds found in ginger, 6-gingerol, and 6-paradol at reducing survival of
prostate cancer cells and reducing STAT3 and NF-kB signaling. 6-SHO also showed
significant tumor growth inhibitory activity in an allograft model using HMVP2
cells. Overall, the current results suggest that 6-SHO may have potential as a
chemopreventive and/or therapeutic agent for prostate cancer and that further study
of this compound is warranted.
AN - WOS:000337182700008
AU - Saha, A.
AU - Blando, J.
AU - Silver, E.
AU - Beltran, L.
AU - Sessler, J.
AU - DiGiovanni, J.
DA - JUN
DO - 10.1158/1940-6207.CAPR-13-0420
IS - 6
PY - 2014
SN - 1940-6207
1940-6215
SP - 627-638
ST - 6-Shogaol from Dried Ginger Inhibits Growth of Prostate Cancer Cells Both In
Vitro and In Vivo through Inhibition of STAT3 and NF-kappa B Signaling
T2 - CANCER PREVENTION RESEARCH
TI - 6-Shogaol from Dried Ginger Inhibits Growth of Prostate Cancer Cells Both In
Vitro and In Vivo through Inhibition of STAT3 and NF-kappa B Signaling
VL - 7
ID - 6475
ER -
TY - JOUR
AB - Excessive reactive oxygen species (ROS) and unresolved inflammations are the
major causes of impaired wound healing as they overwhelm the cellular antioxidant
system and impede the healing process. In this study, we examined the application
of Prussian blue (PB) nanozyme as a novel material for cutaneous wound healing
through the alleviation of excessive ROS and inflammation modulation. The PB
nanoparticles not only exhibited hydrogen peroxide (H2O2) degradation activity but
also showed strong superoxide scavenging ability. PB nanozyme mitigated the
intracellular ROS at a high oxidative stress environment, resulting in a pronounced
cytoprotective effect. Moreover, PB nanozyme also displayed significant anti-
inflammatory activity, as evident from the suppression of inflammatory mediators in
the lipopolysaccharide (LPS) induced macrophage cells. Encouraged by the in vitro
results, we evaluated the in vivo therapeutic efficacy of PB nanozyme in a full
thickness cutaneous wound model combined with LPS treatment to mimic bacterial
infection. The beneficial effects of topically applied PB nanozyme on wound healing
and tissue regeneration were evident compared to the control. The periodical
administration of a low amount (50 mu g x 4) of PB nanoparticles exhibited faster
wound closure as well as collagen deposition, maturation, and organization.
Moreover, the PB treatment effectively induced the differentiation of
keratinocytes, enhanced the neovascularization, and reduced macrophage burden in
the entire wound site. Thus, PB nanozyme not only accelerated the healing process
in an infection-mimicking cutaneous wound model but also exhibited tissue
regeneration characteristics owing to the synergistic effect of ROS-scavenging and
anti-inflammatory activities.
AN - WOS:000600892300002
AU - Sahu, A.
AU - Jeon, J.
AU - Lee, M. S.
AU - Yang, H. S.
AU - Tae, G.
C7 - 111596
DA - FEB
DO - 10.1016/j.msec.2020.111596
PY - 2021
SN - 0928-4931
1873-0191
ST - Antioxidant and anti-inflammatory activities of Prussian blue nanozyme
promotes full-thickness skin wound healing
TI - Antioxidant and anti-inflammatory activities of Prussian blue nanozyme
promotes full-thickness skin wound healing
VL - 119
ID - 6074
ER -
TY - JOUR
AB - Inulin is a versatile, water-soluble polysaccharide that is commonly
available in nature. In the pharmaceutical industry, the non-digestible function of
inulin has made it attractive. Inulin is granted with GRAS status by the FDA and
more than 30,000 plants in nature store inulin as a carbohydrate. The chicory is
the key plant source of inulin out of all sources. It can be used as the sugar or
fat replacer in the processed foods to influence the desirable characteristics.
Good biocompatibility, essential chemical properties, and a wide variety of
bioactivities have rendered inulin an outstanding natural nutrient. Regulating
blood sugar, antioxidant, anticancer is some of the biological activities of
inulin. Inulin can also be a carrier for colon/tumor targeting, as only specific
enzymes in the colon zhydrolyze the inulin. It allows the growth of micro-flora,
the good bacteria in the gut. Inulin is considered as a prebiotic as it is
fermented by bacteria that normalize the colon. This review offers an in-depth
insight into its novel Pharmaceutical applications as well as sources, processing,
physicochemical properties, and nutritional and physiological activities. The
chemically modified inulin is gaining a specific interest in the pharmaceutical
field with its outstanding properties which are discussed in this review. © 2021
The Authors. Pnces Pvt Ltd.
AU - Sai Kishan, C.
AU - Akhila, A. R.
AU - Sahoo, S.
AU - Kulkarni, P. K.
DB - Scopus
DO - 10.22159/ijap.2021v13i3.40863
IS - 3
KW - Inulin
Pharmaceutical applications
Prebiotics
acrylic acid
alcohol
antioxidant
aspartame
calcium
calcium ion
carbohydrate
carbon dioxide
catechin
cholesterol
dextran
dextran sulfate
doxorubicin
epirubicin
fenofibrate
free radical
fructose oligosaccharide
galactose oligosaccharide
gastrointestinal hormone
glucose
glucose derivative
gold nanoparticle
hemagglutinin
hemoglobin A1c
hot water
hydrogel
indometacin
influenza vaccine
intestine enzyme
inulin
inulinase
irbesartan
irinotecan
lactic acid
lactulose
lipoprotein
magnesium
mannitol
methotrexate
microsphere
nanoparticle
oligomer
ornidazole
ornithine decarboxylase
paclitaxel
poloxamer
polymer
polysaccharide
polysorbate
prebiotic agent
short chain fatty acid
silver nanoparticle
sorbitol
stabilizing agent
sucrose
sweetening agent
tanshinone
tetrahydrocannabinol
trehalose
triacylglycerol
aberrant crypt focus
acquired immune deficiency syndrome
anorexia
atherosclerosis
bacterium
biocompatibility
biological activity
body weight loss
Caco-2 cell line
calcium blood level
chicory
colon
colon cancer
colon cell line
colorectal cancer
constipation
Crohn disease
crystal
diabetes mellitus
dietary fiber
drug industry
drug release
drug solubility
drug stability
drug therapy
energy metabolism
enteropathy
estimated glomerular filtration rate
evaporation
extraction
fatty liver
fermentation
freeze drying
freezing point
glucagon release
glucose blood level
heart disease
hydrolysis
hypercholesterolemia
hyperlipidemia
hypertension
hypertriglyceridemia
hypoglycemia
hypothalamus
immune system
Influenza virus
intestine
intestine flora
intestine lymphatic tissue
intestine mucosa
Lactobacillus
large intestine
lipogenesis
magnesium blood level
metabolic activity assay
micelle
microbial growth
microfiltration
microflora
natural killer cell
neuroblastoma
nonhuman
nutritional value
obesity
pancreas cancer
phagocytosis
physical chemistry
polymerization
pulsed electric field
purification
rectum cancer
Review
shelf life
skin cancer
small intestine
systematic review
T lymphocyte
thermostability
triacylglycerol blood level
ulcerative colitis
ultrasound
upper gastrointestinal tract
vaccination
viscosity
wettability
M3 - Review
PY - 2021
SP - 30-38
ST - A comprehensive review on pharmaceutical and nutritional applications of
inulin
T2 - International Journal of Applied Pharmaceutics
TI - A comprehensive review on pharmaceutical and nutritional applications of
inulin
85107015288&doi=10.22159%2fijap.2021v13i3.40863&partnerID=40&md5=8643bd6e01f1844017
c3282b987bdeaa
VL - 13
ID - 5164
ER -
TY - JOUR
AB - Tuberculosis remains one of the top three leading causes of morbidity and
mortality worldwide, complicated by the emergence of drug-resistant Mycobacterium
tuberculosis strains and high rates of HIV coinfection. It is important to develop
new antimycobacterial drugs and immunomodulatory therapeutics and compounds that
enhance antituberculous immunity. Dipterinyl calcium pentahydrate (DCP), a calcium-
complexed pterin compound, has previously been shown to inhibit human breast cancer
cells and hepatitis B virus (HBV). DCP inhibitory effects were attributed to
induction of apoptosis and/or increased production of interleukin 12 (IL-12) and
granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study, we tested
the ability of DCP to mediate inhibition of intracellular mycobacteria within human
monocytes. DCP treatment of infected monocytes resulted in a significant reduction
in viability of intracellular but not extracellular Mycobacterium bovis BCG. The
antimicrobial activity of DCP was comparable to that of pyrazinamide (PZA), one of
the first-line antituberculosis drugs currently used. DCP potentiated monocyte
antimycobacterial activity by induction of the cysteine-cysteine (C-C) chemokine
macrophage inflammatory protein 1 beta (MIP-1 beta) and inducible nitric oxide
synthase 2. Addition of human anti-MIP-1 beta neutralizing antibody or a specific
inhibitor of the L-arginase-nitric oxide pathway (N-G-monomethyl L-arginine [L-
NMMA] monoacetate) reversed the inhibitory effects of DCP on intracellular
mycobacterial growth. These findings indicate that DCP induced mycobacterial
killing via MIP-1 beta- and nitric oxide-dependent effects. Hence, DCP acts as an
immunoregulatory compound enhancing the antimycobacterial activity of human
monocytes.
AN - WOS:000318855100011
AU - Sakala, I. G.
AU - Eickhoff, C. S.
AU - Blazevic, A.
AU - Moheno, P.
AU - Silver, R. F.
AU - Hoft, D. F.
DA - JUN
DO - 10.1128/IAI.01393-12
IS - 6
PY - 2013
SN - 0019-9567
1098-5522
SP - 1974-1983
ST - Dipterinyl Calcium Pentahydrate Inhibits Intracellular Mycobacterial Growth
in Human Monocytes via the C-C Chemokine MIP-1 beta and Nitric Oxide
TI - Dipterinyl Calcium Pentahydrate Inhibits Intracellular Mycobacterial Growth
in Human Monocytes via the C-C Chemokine MIP-1 beta and Nitric Oxide
VL - 81
ID - 6827
ER -
TY - JOUR
AB - A nanohybrid formulation of silver-titanium dioxide nanoparticles/poly(lactic
acid) (Ag-TiO2/PLA) was designed for Norfloxacin/Tenoxicam (NOR/TENO) targeted
delivery to maximize the bioavailability and minimize the side effects of the
drugs. Ag-TiO2 nanoparticles were prepared via Stober method. NOR, TENO and a
mixture of NOR/TENO (NT) were loaded onto Ag-TiO2 nanoparticles and coated by PLA
via solution casting. The physical interaction between the drugs and carrier was
confirmed by Fourier-transform infrared (FTIR) analysis. X-ray diffraction (XRD)
demonstrated that Ag-TiO2 consists of a cubic phase of Ag with two phases of TiO2
(anatase and brookite). Ag nanoparticle fine spots coated with TiO2 were collected
to form spheres averaging at 100 nm in size. In-vitro release behavior of drugs was
studied at different pH (5.4, 7.4) and the release of drug from NT/Ag-TiO2/PLA was
faster at pH 7.4. Gram-positive and Gram-negative bacteria were used to investigate
antibacterial properties of the nanohybrid. Cytotoxicity of the nanohybrid using an
MTT assay was studied against different tumor and normal cell lines. It was found
that NT/Ag-TiO2/PLA has an excellent cytotoxic effect against various bacterial
cells and tumor cell lines. In addition, antioxidant properties of the nanohybrids
were tested using ABTS method and the nanohybrid showed moderate antioxidant
activity. © 2021
AU - Salahuddin, N.
AU - Gaber, M.
AU - Elneanaey, S.
AU - Snowdon, M. R.
AU - Abdelwahab, M. A.
DB - Scopus
DO - 10.1016/j.ijbiomac.2021.03.033
KW - Nanohybrids
Norfloxacin
Targeted delivery
Tenoxicam
Cell Line
Cell Survival
Drug Liberation
Hep G2 Cells
Humans
Metal Nanoparticles
Piroxicam
Polyesters
Silver
Spectrophotometry
Titanium
brookite
nanocarrier
norfloxacin
norfloxacin plus tenoxicam
polylactic acid
silver nanoparticle
tenoxicam
titanium dioxide
unclassified drug
antiinfective agent
metal nanoparticle
piroxicam
polyester
polylactide
silver
titanium
Article
Bacillus cereus
drug release
drug safety
Escherichia coli
excipient compatibility
HCT 116 cell line
Hep-G2 cell line
in vitro study
MCF-7 cell line
MTT assay
pH
Proteus vulgaris
Streptococcus agalactiae
X ray diffraction
cell line
cell survival
chemistry
drug effect
human
procedures
spectrophotometry
ultrastructure
M3 - Article
PY - 2021
SP - 771-781
ST - Co-delivery of norfloxacin and tenoxicam in Ag-TiO2/poly(lactic acid)
nanohybrid
TI - Co-delivery of norfloxacin and tenoxicam in Ag-TiO2/poly(lactic acid)
nanohybrid
85103099469&doi=10.1016%2fj.ijbiomac.2021.03.033&partnerID=40&md5=b5491844f54c74270
8da8c526118d151
VL - 180
ID - 5179
ER -
TY - JOUR
AB - The impact of household processes on fenugreek leaves and seeds has been
analyzed for total phenolic (TP) and total flavonoid content (TF), and in-vitro
biological activities such as antioxidant, antimicrobial, and anti-inflammatory
properties. Processes included air-drying for leaves and germinating, soaking, and
boiling for seeds. Air-dried fenugreek leaves (ADFL) had high TP (15.27 mg GAE g(-
1) D.W.) and TF (7.71 mg QE g(-1) D.W.) (milligram quercetin equivalents per gram
dry weight). The TP contents of unprocessed, germinated, soaked, and boiled seeds
were 6.54, 5.60, 4.59, and 3.84 mg gallic acid equivalents per gram of dry weight
(mg GAE g(-1) D.W.), respectively. The TF contents in unprocessed fenugreek seeds,
germinated fenugreek seeds, soaked fenugreek seeds, and boiled fenugreek seeds
(BFS) were 4.23, 2.11, 2.10, and 2.33 mg QE g(-1) D.W., respectively. Sixteen
phenolic and nineteen flavonoid compounds has been identified using high-
performance liquid chromatography. Antioxidant activity using 2,2-diphenyl-1-
picrylhydrazil (DPPH center dot), 2,2-azinobis (3-ethylbenothiazoline-6-sulfonic
acid (ABTS(+center dot)), and ferric reducing antioxidant power (FRAP(center dot))
assays indicated that ADFL had the highest activity. Antimicrobial activity has
been evaluated against each of the eight pathogenic bacterial and fungal strains.
ADFL showed the strongest activity with minimum inhibitory concentrations values
ranging from 0.03 to 1.06 and 0.04 to 1.18 mg ml(center dot 1) against bacterial
and fungal strains, respectively. Anti-inflammatory activity was evaluated in-vitro
against RAW 264.7 macrophage cells using the nitric oxide (NO) assay. Results
revealed that ADFL had the highest cytotoxicity and anti-inflammatory activity
according to the NO assay. Household processes significantly reduced the in-vitro
biological properties of processed seeds.
AN - WOS:000984763000018
AU - Salam, S. G. A.
AU - Rashed, M. M.
AU - Ibrahim, N. A.
AU - Rahim, E. A. A.
AU - Aly, T. A. A.
AU - Al-Farga, A.
DA - APR 29
DO - 10.1038/s41598-023-31888-y
IS - 1
PY - 2023
SN - 2045-2322
ST - Phytochemical screening and in-vitro biological properties of unprocessed and
household processed fenugreek (Trigonella foenum-graecum Linn.) seeds and leaves
TI - Phytochemical screening and in-vitro biological properties of unprocessed and
household processed fenugreek (Trigonella foenum-graecum Linn.) seeds and leaves
VL - 13
ID - 6550
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are the most common nanomaterials in consumer
products. Therefore, it has been crucial to control AgNPs toxicological effects to
improve their safety and increase the outcome of their applications. This work
investigated the possible protective effect of thymoquinone (TQ) against AgNPs-
induced hepatic and renal cytotoxicity in rats. Serum markers of liver and kidney
functions as well as liver and kidney oxidative stress status, pro-inflammatory
cytokines, apoptosis markers, and histopathology were assessed. TQ reversed AgNPs-
induced elevation in serum liver and kidney function markers, including aspartate
transaminase, alanine transaminase, urea, and creatinine. Moreover, TQ co-
administration with AgNPs alleviates hepatic and renal oxidative insults by
decreasing MDA and NO levels with a significant increase in the activity of
antioxidant enzymes (superoxide dismutase, catalase, and glutathione recycling
enzymes peroxidase and reductase) compared to AgNPs-treated rats. Besides, TQ
upregulated hepatic and renal Nrf2 gene expression in AgNPs-intoxicated rats.
Furthermore, TQ co-administration decreased the hepatic and renal pro-inflammatory
mediators represented by IL-1β, TNF-α, TGF-β, and NF-κB levels. Besides, TQ co-
administration decreased apoptotic protein (Bax) levels and increased the anti-
apoptotic protein (Bcl-2) levels. These findings were confirmed by the
histopathological examination of hepatic and renal tissues. Our data affirmed the
protective effect of TQ against AgNPs cytotoxicity and proposed a possible
mechanism of TQ antioxidant, anti-inflammatory, and anti-apoptotic effects.
Consequently, we could conclude that using TQ might control AgNPs toxicological
effects, improve their safety, and increase the outcome of their applications. ©
2022, The Author(s), under exclusive licence to Springer Science+Business Media,
LLC, part of Springer Nature.
AU - Salama, B.
AU - Alzahrani, K. J.
AU - Alghamdi, K. S.
AU - Al-Amer, O.
AU - Hassan, K. E.
AU - Elhefny, M. A.
AU - Albarakati, A. J. A.
AU - Alharthi, F.
AU - Althagafi, H. A.
AU - Al Sberi, H.
AU - Amin, H. K.
AU - Lokman, M. S.
AU - Alsharif, K. F.
AU - Albrakati, A.
AU - Abdel Moneim, A. E.
AU - Kassab, R. B.
AU - Fathalla, A. S.
DB - Scopus
DO - 10.1007/s12011-022-03399-w
IS - 6
KW - Apoptosis
Hepatorenal Toxicity
Inflammation
Oxidative Damage
ThyMoquinone
Animals
Antioxidants
Benzoquinones
Kidney
Liver
Metal Nanoparticles
Oxidative Stress
Rats
Silver
3,4 methylenedioxyamphetamine
catalase
creatinine
cytokine
glutathione
interleukin 1beta
nitric oxide
oxidoreductase
peroxidase
protein Bax
protein bcl 2
silver nanoparticle
thymoquinone
urea
antioxidant
benzoquinone derivative
metal nanoparticle
silver
adult
animal experiment
apoptosis
Article
controlled study
cytotoxicity
drug safety
gene expression
histopathology
human
inflammation
kidney function
kidney tissue
liver function
liver tissue
liver toxicity
male
nephrotoxicity
nonhuman
oxidative stress
rat
urea blood level
animal
kidney
liver
metabolism
M3 - Article
PY - 2023
SP - 2942-2954
ST - Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in
Liver and Kidney Tissues: Potential Protective Role of Thymoquinone
TI - Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in
85138005255&doi=10.1007%2fs12011-022-03399-
w&partnerID=40&md5=be0b4cd6fd4d4d481a7aff215766167f
VL - 201
ID - 5014
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are the most common nanomaterials in consumer
products. Therefore, it has been crucial to control AgNPs toxicological effects to
improve their safety and increase the outcome of their applications. This work
investigated the possible protective effect of thymoquinone (TQ) against AgNPs-
induced hepatic and renal cytotoxicity in rats. Serum markers of liver and kidney
functions as well as liver and kidney oxidative stress status, pro-inflammatory
cytokines, apoptosis markers, and histopathology were assessed. TQ reversed AgNPs-
induced elevation in serum liver and kidney function markers, including aspartate
transaminase, alanine transaminase, urea, and creatinine. Moreover, TQ co-
administration with AgNPs alleviates hepatic and renal oxidative insults by
decreasing MDA and NO levels with a significant increase in the activity of
antioxidant enzymes (superoxide dismutase, catalase, and glutathione recycling
enzymes peroxidase and reductase) compared to AgNPs-treated rats. Besides, TQ
upregulated hepatic and renal Nrf2 gene expression in AgNPs-intoxicated rats.
Furthermore, TQ co-administration decreased the hepatic and renal pro-inflammatory
mediators represented by IL-1 beta, TNF-alpha, TGF-beta, and NF-kappa B levels.
Besides, TQ co-administration decreased apoptotic protein (Bax) levels and
increased the anti-apoptotic protein (Bcl-2) levels. These findings were confirmed
by the histopathological examination of hepatic and renal tissues. Our data
affirmed the protective effect of TQ against AgNPs cytotoxicity and proposed a
possible mechanism of TQ antioxidant, anti-inflammatory, and anti-apoptotic
effects. Consequently, we could conclude that using TQ might control AgNPs
toxicological effects, improve their safety, and increase the outcome of their
applications.
AN - WOS:000844949500002
AU - Salama, B.
AU - Alzahrani, K. J.
AU - Alghamdi, K. S.
AU - Al-Amer, O.
AU - Hassan, K. E.
AU - Elhefny, M. A.
AU - Albarakati, A. J. A.
AU - Alharthi, F.
AU - Althagafi, H. A.
AU - Al Sberi, H.
AU - Amin, H. K.
AU - Lokman, M. S.
AU - Alsharif, K. F.
AU - Albrakati, A.
AU - Moneim, A. E. A.
AU - Kassab, R. B.
AU - Fathalla, A. S.
C6 - AUG 2022
DA - JUN
DO - 10.1007/s12011-022-03399-w
IS - 6
PY - 2023
SN - 0163-4984
1559-0720
SP - 2942-2954
ST - Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in
TI - Silver Nanoparticles Enhance Oxidative Stress, Inflammation, and Apoptosis in
VL - 201
ID - 5854
ER -
TY - JOUR
AB - No ideal cross-linking agent has been identified for decellularized livers
(DLs) yet. In this study, we evaluated structural improvements and biocompatibility
of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver
slices were decellularized and then loaded with AgNPs (100 nm) after optimization
of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular
carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking
effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl
carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that
AgNPs improved the ultra-structure of DLs’ collagen fibres with good porosity and
increased DLs’ resistance against in vitro degradation with good cytocompatibility.
AgNPs decreased the host inflammatory reaction against implanted porcine DL slices
in vivo and increased the polarization of M2 macrophages. Thus, structural and
functional improvements of Porcine DLs could be achieved using AgNPs. © 2018, ©
AU - Saleh, T.
AU - Ahmed, E.
AU - Yu, L.
AU - Hussein, K.
AU - Park, K. M.
AU - Lee, Y. S.
AU - Kang, B. J.
AU - Choi, K. Y.
AU - Choi, S.
AU - Kang, K. S.
AU - Woo, H. M.
DB - Scopus
DO - 10.1080/21691401.2018.1457037
IS - sup2
cross-linking
decellularization
Liver
tissue engineering
Animals
Collagenases
Hep G2 Cells
Humans
Materials Testing
Metal Nanoparticles
Silver
Swine
Biocompatibility
Biodegradation
Cell culture
Crosslinking
Endothelial cells
Metal nanoparticles
Stability
Tissue engineering
beta actin
collagen
complementary DNA
cyanamide
DNA
elastin
glutaraldehyde
n hydroxysuccinimide
silver nanoparticle
collagenase
metal nanoparticle
silver
Carbodiimide hydrochlorides
Decellularization
Degradation resistance
Functional improvements
Hepatocellular carcinoma
Structural improvements
animal cell
animal experiment
animal tissue
Article
biodegradation
collagen fiber
comparative study
controlled study
cross linking
EA.hy 926 cell line
Hep-G2 cell line
inflammation
liver slice
liver structure
liver tissue
macrophage
nonhuman
pig
porosity
process optimization
animal
chemistry
cytology
dose response
drug effect
human
liver
materials testing
metabolism
M3 - Article
PY - 2018
SP - 273-284
ST - Silver nanoparticles improve structural stability and biocompatibility of
decellularized porcine liver
TI - Silver nanoparticles improve structural stability and biocompatibility of
decellularized porcine liver
85044448643&doi=10.1080%2f21691401.2018.1457037&partnerID=40&md5=47b506b0def2c7a64e
eca5039989efc6
VL - 46
ID - 5457
ER -
TY - JOUR
AB - Prodigiosin is a red pigment produced by Serratia marcescens strain.
Bacterial prodigiosin and its synthetic derivatives are efficacious antioxidants
and proapoptotic agents. This study illustrates a new approach for use of
prodigiosin conjugated silver nanoparticles (PG-AgNP2) against cadmium chloride
(CdCl2) induced neurotoxicity in rats. Rats were (ip) injected with Cd (6.5 mg/kg)
for 7 days with or without PG-AgNP2 (3 mg/kg). The concentration of Cd, DA, NE, 5-
HT, amino acids, NO, MDA, SOD, GSH, catalase, TNF-α, IL-6, Bax, Bcl2 and Caspase-3.
The Cd-intoxicated group showed a significant increase in Cd concentration in brain
tissue, in addition, to an increase in MDA and NO and a decrease in the content of
neurotransmitters (DA, NE, and 5-HT), inhibitory amino acids, and level of all
studied antioxidant enzymes. PG-AgNP2 treatment, significantly reduced Cd-induced
brain tissue injury as indicated by increased antioxidant molecules,
neurotransmitters (DA, NE, and 5-HT), and inhibitory amino acids accompanied by
lower oxidative stress indices (MDA and NO) and excitatory amino acids in brain
tissue. PG-AgNP2 decreased inflammatory mediators including pro-inflammatory
cytokines and prevented the development of apoptosis in the brain tissue. Our
findings suggest that PG-AgNP2 can act as a therapeutic agent against neuronal
impairments associated with Cd exposure. © 2022, Sociedade Brasileira de Ciencia e
Tecnologia de Alimentos, SBCTA. All rights reserved.
AU - Salem, F. E.
AU - Yehia, H. M.
AU - Korany, S. M.
AU - Alarjani, K. M.
AU - Al-Masoud, A. H.
AU - Elkhadragy, M. F.
C7 - e97322
DB - Scopus
DO - 10.1590/fst.97322
KW - brain
cd toxicity
nanoparticles
neurotransmitters
prodigiosin
M3 - Article
PY - 2022
ST - Neurotherapeutic effects of prodigiosin conjugated with silver-nanoparticles
in rats exposed to cadmium chloride-induced neurotoxicity
T2 - Food Science and Technology (Brazil)
TI - Neurotherapeutic effects of prodigiosin conjugated with silver-nanoparticles
in rats exposed to cadmium chloride-induced neurotoxicity
85139978986&doi=10.1590%2ffst.97322&partnerID=40&md5=260e3579bf57b30b39e9fdc1e23b3a
b6
VL - 42
ID - 5071
ER -
TY - JOUR
AB - Silver and silver oxides are gaining interest in medical applications for
their prominent antibacterial and antimicrobial potentials. Recent studies suggest
that nanosilver oxide has remarkable anti-inflammatory effects and enhances wound
healing. Nevertheless, its effect on gastric ulcer has not yet been illustrated.
Thus the current study aimed to explore the prospect protective effect of
nanosilver oxide against indomethacin-induced gastric ulcer. A new approach has
been followed to synthesize nanosilver oxide. X-ray diffraction, UV–Vis
spectroscopy and transition electron microscope techniques have been successfully
used to characterize the synthesized nanoparticles. Treatment of ulcerated rats
with different doses of nanosilver oxide especially (175 and 350 ppm/p.o.)
alleviated adverse effects of indomethacin-induced gastric injury as demonstrated
by decreasing ulcer index and elevating % of ulcer inhibition. These positive
effects excelled those exerted by the reference antiulcer drug omeprazole.
Nanosilver oxide suppressed gastric inflammation by reducing myeloperoxidase, tumor
necrosis alpha, interleukin 1beta and interferon gamma. Moreover, nanosilver oxide
halted gastric oxidative stress via inhibiting lipid peroxidation and enhancing
glutathione and paraoxonase-1. Regarding gastric apoptosis, nanosilver oxide down
regulated the expression of caspase 9, tumor protein 53, and nuclear factor kappa B
and allograft inflammatory factor-1 genes. These findings emphasize the
antiulcerogenic potential of nanosilver oxide against indomethacin-induced gastric
ulcers which are multi-factorial including anti-inflammatory, antioxidant and
antiapoptotic effects. © 2017, Springer International Publishing AG, part of
Springer Nature.
AU - Salem, N. A.
AU - Wahba, M. A.
AU - Eisa, W. H.
AU - El-Shamarka, M.
AU - Khalil, W.
DB - Scopus
DO - 10.1007/s10787-017-0424-2
IS - 4
KW - Apoptosis
Gastric ulcer
Indomethacin
Inflammation
Oxidative stress
Silver oxide nanoparticles
Animals
Anti-Ulcer Agents
Antioxidants
Lipid Peroxidation
Male
Metal Nanoparticles
Oxidative Stress
Oxides
Rats
Rats, Wistar
Silver Compounds
Stomach Ulcer
X-Ray Diffraction
antiulcer agent
aryldialkylphosphatase 1
caspase 9
complementary DNA
gamma interferon
glutathione
indometacin
interleukin 1beta
lead
metal nanoparticle
myeloperoxidase
omeprazole
pepzol
protein p53
RNA
silver oxide nanoparticle
unclassified drug
antioxidant
disilver oxide
oxide
silver derivative
animal experiment
animal model
animal tissue
apoptosis
Article
cell separation
controlled study
down regulation
drug mechanism
gene expression
indomethacin-induced gastric ulcer
leukocyte
lipid peroxidation
male
neutrophil
nonhuman
oxidative stress
priority journal
rat
single drug dose
ulcer index
X ray diffraction
animal
chemically induced
dose response
drug effect
stomach ulcer
Wistar rat
M3 - Article
PY - 2018
SP - 1025-1035
ST - Silver oxide nanoparticles alleviate indomethacin-induced gastric injury: a
novel antiulcer agent
T2 - Inflammopharmacology
TI - Silver oxide nanoparticles alleviate indomethacin-induced gastric injury: a
novel antiulcer agent
85036575874&doi=10.1007%2fs10787-017-0424-
2&partnerID=40&md5=e953274bc02b5021580581609f05fc0f
VL - 26
ID - 5415
ER -
TY - JOUR
AB - Cynara humilis is traditionally used to treat skin burns and microbial
infections. However, experimental studies on this plant are rare. Furthermore, the
aim of this study was to investigate the effects of Cynara humilis, a Moroccan
herbal remedy, on the healing of deep second-degree burns in rats with a silver
sulfadiazine group. This research was also carried out to confirm if C. humilis had
antibacterial capabilities. Under typical burn procedures, each rat received a deep
second-degree burn on the upper back. The burns were treated regularly with control
groups (control and control VH), silver sulfadiazine (SDD) in group 3, C. humilis
ethanolic extract (CHEE) in group 4, and C. humilis aqueous extract (CHAE) in group
5. Throughout the treatment, digital photography was used to measure rat responses
to the treatment until day 18. After the scar biopsy at the end of the study,
histological parameters (inflammatory cells, collagen, epithelialization, fibrosis,
and granulation tissue) were assessed. Using the well technique, the antibacterial
activity of the extracts was tested against Staphylococcus aureus CIP 483, Bacillus
subtilis CIP 5262, Escherichia coli CIP 53126, Pseudomonas aeruginosa CIP 82118,
and Salmonella enterica CIP 8039, and the results showed important activities of
the ethanolic and aqueous extracts against the five species tested with MICs of 2
and 4 mg/mL, respectively. In the aqueous extract group, the wound healed faster.
In addition, the healing rate in the C. humilis extracts (CHEA and CHEE) group was
faster than in the silver sulfadiazine and control groups. In the C. humilis group,
maximum wound surface recovery was observed at the same time, as it was not noted
in the silver sulfadiazine group. Pathologically, epithelialization was more marked
in wounds treated with C. humilis extracts (CHE). Angiogenesis and inflammatory
cells were considerably lower in the CHE group than in the silver and other control
groups. However, elastic fibers were considerable in the CHE-treated group. In
histological examination, the C. humilis group had a low incidence of angiogenesis
and inflammation, indicating that this group had less wound scarring. Collagen and
burn wound healing were both faster in the C. humilis group. The findings of this
study suggest that C. humilis, as indicated by traditional medicine, is a promising
natural source for the management of wound healing. © 2023 Najoua Salhi et al.
AU - Salhi, N.
AU - El Guourrami, O.
AU - Rouas, L.
AU - Moussaid, S.
AU - Moutawalli, A.
AU - Benkhouili, F. Z.
AU - Ameggouz, M.
AU - Alshahrani, M. M.
AU - Al Awadh, A. A.
AU - Bouyahya, A.
AU - Faouzi, M. E. A.
AU - Cherrah, Y.
C7 - 5855948
DB - Scopus
DO - 10.1155/2023/5855948
KW - chloramphenicol
cynara humilis extract
ketamine
plant extract
resazurin
sulfadiazine silver
unclassified drug
angiogenesis
animal cell
animal experiment
animal model
Article
Bacillus subtilis
biopsy
burn
controlled study
Cynara
drug efficacy
epithelization
Escherichia coli
female
herbal medicine
image processing
male
nonhuman
rat
Salmonella enterica
skin toxicity
tissue section
wound healing
M3 - Article
PY - 2023
ST - Evaluation of the Wound Healing Potential of Cynara humilis Extracts in the
Treatment of Skin Burns
T2 - Evidence-based Complementary and Alternative Medicine
TI - Evaluation of the Wound Healing Potential of Cynara humilis Extracts in the
Treatment of Skin Burns
85158069477&doi=10.1155%2f2023%2f5855948&partnerID=40&md5=5d3b86db5747d89dc72b1a398
d34d478
VL - 2023
ID - 5029
ER -
TY - JOUR
AB - Objective: This study was set up to evaluate the efficacy of a concentrated
surfactant-based wound dressing (with and without silver sulfadiazine [SSD]) on
wound repair, by investigating their ability to enhance human dermal fibroblast
proliferation and viability. In addition, the wound dressings were evaluated for
their ability to suppress biofilms in a three-dimensional (3D) in vitro wound
biofilm model and modulate the inflammatory cytokine interleukin-6 (IL-6) and tumor
necrosis factor-Alpha (TNFα). Approach: Problematic biofilms are well known to
affect fibroblast and keratinocyte viability. To assess wound repair and
inflammatory cytokine modulation, a direct cytotoxicity assay and a 3D
keratinocyte-fibroblast model were employed. Results: At 1 and 7 days
posttreatment, the non-Antimicrobial dressing was noncytotoxic and the
antimicrobial dressing was moderately cytotoxic to adult human dermal fibroblasts
cells. Within the 3D wound model, the biofilm demonstrated a decelerating effect on
wound closure and a decrease in viable cells. When the non-Antimicrobial-and
antimicrobial-based concentrated surfactant-based wound dressing was applied to the
wound model, reduced biofilm was observed. The application of wound dressings to
the biofilm-infected wound also resulted in a reduction of IL-6 and TNFα. The
concentrated surfactant-based wound dressing without an antimicrobial was shown to
enhance cellular viability and migration. Innovation and Conclusion: We have
demonstrated the ability of a surfactant-based wound dressing to minimize the
deleterious effects of a wound biofilm, modulate the secretion of inflammatory
cytokines, and enhance cellular proliferation in a biofilm-infected wound model.
Furthermore, the non-Antimicrobial-based concentrated surfactant dressings did not
affect cellular viability and therefore represents a multifaceted approach to the
treatment of wounds infected with biofilms. © Copyright 2018, Mary Ann Liebert,
Inc. 2018.
AU - Salisbury, A. M.
AU - Mayer, D.
AU - Chen, R.
AU - Percival, S. L.
DB - Scopus
DO - 10.1089/wound.2017.0782
IS - 9
KW - biofilm
biofilm control
cytotoxicity
surfactant
wound
wound dressing
wound healing
interleukin 6
sulfadiazine silver
adult
Article
cell adhesion
cell migration
cell structure
cell viability
comparative study
controlled study
cytokine release
cytotoxicity assay
fibroblast
histology
human
human cell
human cell culture
in vitro study
keratinocyte
priority journal
wound closure
M3 - Article
PY - 2018
SP - 315-322
ST - Efficacy of Concentrated Surfactant-Based Wound Dressings in Wound Repair and
Biofilm Reduction
T2 - Advances in Wound Care
TI - Efficacy of Concentrated Surfactant-Based Wound Dressings in Wound Repair and
Biofilm Reduction
85053136353&doi=10.1089%2fwound.2017.0782&partnerID=40&md5=0b97b35aea3980e17ab114a5
b3c906ad
VL - 7
ID - 5439
ER -
TY - JOUR
AB - Various assisted reproductive technologies (ART) are applied in the
infertility treatment. However, considerable attention is addressed to use
alternative approaches, such as non-biotechnology, probiotics, and traditional
medicinal plants to treat cancerous and non-cancerous cases of infertility.
Nanotechnology was remarkably aided in treatment, diagnosis, and drug delivery. The
existing data of this technology might demonstrate the enormous potential of
nanomaterials and their viability in clinical trials for the study of reproductive
issues. In order to understand the function of the microbiome in infertility and
the many good effects of probiotics in illnesses such as colon cancer, obesity,
diabetes, and inflammatory bowel disease, research on infertility must be
conducted. Healthy reproductive systems are important for successful fertility in
males and females, and using probiotics can help reduce the associated
complications. Besides, in vivo models are required to determine the probiotics
proper administration, identify the functional species, effective doses,
administration forms, and the effects of their combination with conventional
antibiotics. In addition, medicinal herbs should be explored, notably in the
treatment of male infertility and the improvement of sperm abnormalities. The
antioxidant capacity, anti-inflammatory reactions, increased sperm production, and
increased testosterone levels in the blood are all examples of medicinal plant
benefits. More study is needed to establish specific findings on which substances
are involved and have effective and safe fertility potential. This review presents
an overview of potential applications for nanotechnology, probiotics, and medicinal
plants in infertility, discussing the advantages, their feasibility, and associated
concerns, which demand more investigations to set of clinical applications. © 2023
Bentham Science Publishers.
AU - Salmany, N.
AU - Lotfi, H.
AU - Keyhanmanesh, R.
AU - Ghiasi, R.
C7 - e270422204099
DB - Scopus
DO - 10.2174/1573404818666220427083700
IS - 4
KW - bowel disease
cancer photothermal therapy
Infertility
medicinal plants
nanotechnology
probiotics
antioxidant
cabazitaxel
carboplatin
cisplatin
curcumin
dendrimer
dexamethasone
efavirenz
follitropin
gold nanoparticle
iron oxide
liposome
luteinizing hormone
macrogol
Muellerian inhibiting factor
nanocarrier
nanocomposite
paclitaxel
polymer
probiotic agent
silver nanoparticle
Alzheimer disease
antiviral activity
apoptosis
atherosclerosis
biodegradability
blood brain barrier
Candida albicans
cell death
cell proliferation
chemotherapy
Chlamydia trachomatis
colon cancer
Crocus sativus
diabetes mellitus
drug release
ectopic pregnancy
encapsulation
endometriosis
Escherichia coli
feasibility study
fertility
ginger
Helicobacter pylori
Hepatitis B virus
herbal medicine
human
hysterectomy
infertility
medicinal plant
mouse
Mumps virus
Mycoplasma genitalium
Neisseria gonorrhoeae
nephrotoxicity
neurotoxicity
nonhuman
obesity
osteoporosis
ototoxicity
oxidative stress
pomegranate
prostate cancer
reproductive health
Review
semen analysis
sexual transmission
sperm count
testis cancer
trophoblast
uterine cervix cancer
uterus myoma
vagina flora
vaginitis
velvet bean
Wart virus
M3 - Review
PY - 2023
SP - 91-106
ST - An Overview of Potential Applications for Nanotechnology, Probiotics, and
Medicinal Plants in Infertility Problems
T2 - Current Women's Health Reviews
TI - An Overview of Potential Applications for Nanotechnology, Probiotics, and
Medicinal Plants in Infertility Problems
85149803664&doi=10.2174%2f1573404818666220427083700&partnerID=40&md5=cfbaaccdb6e475
b7026977d9e0757348
VL - 19
ID - 5007
ER -
TY - JOUR
AB - Objective(s): The main objective of the current assay was to evaluate the
antibacterial and regenerative effects of hydrogel nanocomposite containing pure
natural zeolite (clinoptilolite) integrated with alginate (Alg) as wound
healing/dressing biomaterials.Materials and Methods: The zeolites were size
excluded, characterized by SEM, DLS, XRD, FTIR, and XRF, and then integrated into
Alg hydrogel followed by calcium chloride crosslinking. The Alg and alginate
zeolite (Alg/Zeo) hydrogel was characterized by swelling and weight loss tests,
also the antibacterial, hemocompatibility, and cell viability tests were performed.
In animal studies, the burn wound was induced on the back of rats and treated with
the following groups: control, Alg hydrogel, and Alg/Zeo hydrogel.Results: The
results showed that the hydrodynamic diameter of zeolites was 367 +/- 0.2 nm.
Zeolites did not show any significant antibacterial effect, however, the hydrogel
nanocomposite containing zeolite had proper swelling as well as hemocompatibility
and no cytotoxicity was observed. Following the creation of a third-degree burn
wound on the back of rats, the results indicated that the Alg hydrogel and Alg/Zeo
nanocomposite accelerated the wound healing process compared with the control
group. Re-epithelialization, granulation tissue thickness, collagenization,
inflammatory cell recruitment, and angiogenesis level were not significantly
different between Alg and Alg/Zeo nanocomposite.Conclusion: These findings revealed
that although the incorporation of zeolites did not induce a significant beneficial
effect in comparison with Alg hydrogel, using zeolite capacity in hydrogel for
loading the antibiotics or other effective compounds can be considered a promising
wound dressing.
AN - WOS:000988782100013
AU - Samadian, H.
AU - Vahidi, R.
AU - Salehi, M.
AU - Hosseini-Nave, H.
AU - Shahabi, A.
AU - Zanganeh, S.
AU - Lashkari, M.
AU - Kouhbananinejad, S. M.
AU - Kolarijani, N. R.
AU - Amini, S. M.
AU - Asadi-shekari, M.
AU - Parsa, M. J. M.
DA - JUN
DO - 10.22038/IJBMS.2023.68897.15016
IS - 6
PY - 2023
SN - 2008-3866
2008-3874
SP - 708-716
ST - Hydrogel nanocomposite based on alginate/zeolite for burn wound healing: In
vitro and in vivo study
T2 - IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
TI - Hydrogel nanocomposite based on alginate/zeolite for burn wound healing: In
vitro and in vivo study
VL - 26
ID - 6734
ER -
TY - JOUR
AB - Introduction: Silver Nano Particle (SNPs) are the most widely used in
consumer products. Thus, because of increasing potential for exposure of human to
SNPs, there is an increasing concern about possible side effects of this
nanoparticle. Despite the number of studies that have been done, little attention
was paid to the dermal toxicity of these particles on human health. Aim: To
investigate the possible histopathological toxicity at different doses of SNPs in
the liver and kidney of mice. Materials and Methods: In this experimental study, 50
male BALB/c mice of about six weeks were randomly divided into negative control,
positive control, pseudo-control (sham) and two experimental (10 and 100 mu g/mL
SNPs) groups (n=10). After giving general anaesthesia and shaving the back of all
the rats, near the vertebral column, the bandage surface was treated in the
experimental groups, the positive control group, and in pseudo-control group
respectively with a volume of 50 microliters of the SNPs solution (10 and 100 mu
g/mL), AgNO3 solution (100 mu g/mL), and distilled water was added to the sterile
bandage of mice, then the bandages were fixed in the shaved surface, but the
negative control group was without treatment and bandage. After 3 and 7 days,
histologic sampling of liver and kidney of mice were subsequently analysed.
Results: Histopathological studies demonstrated some changes such as dilatation of
the central vein, hyperemia, inflammatory cells in liver, and vacuolar
degeneration, cloudy swelling in kidney. Conclusion: These findings demonstrate
that SNPs (40 nm) can induce hepatotoxicity and nephrotoxicity in mice following
skin absorption in a dose-dependent manner. Therefore, it is necessary to measure
tissue levels of SNPs in all treatment mice. It is also recommended for further
research with various doses for different periods of time following various routes
of administration of SNPs.
AN - WOS:000425431400030
AU - Samani, P. Y.
AU - Arabi, M.
AU - Shadkhast, M.
AU - Piraei, E.
DA - JAN
DO - 10.7860/JCDR/2018/28535.11114
IS - 1
PY - 2018
SN - 2249-782X
0973-709X
SP - CC1-CC4
ST - Repeated-Dose Toxicity in Mouse Liver and Kidney after Skin Exposure to
T2 - JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH
TI - Repeated-Dose Toxicity in Mouse Liver and Kidney after Skin Exposure to
VL - 12
ID - 5997
ER -
TY - JOUR
AB - Introduction: Silver Nano Particle (SNPs) are the most widely used in
consumer products. Thus, because of increasing potential for exposure of human to
SNPs, there is an increasing concern about possible side effects of this
nanoparticle. Despite the number of studies that have been done, little attention
was paid to the dermal toxicity of these particles on human health. Aim: To
investigate the possible histopathological toxicity at different doses of SNPs in
the liver and kidney of mice. Materials and Methods: In this experimental study, 50
male BALB/c mice of about six weeks were randomly divided into negative control,
positive control, pseudo-control (sham) and two experimental (10 and 100 μg/ml
SNPs) groups (n=10). After giving general anaesthesia and shaving the back of all
the rats, near the vertebral column, the bandage surface was treated in the
experimental groups, the positive control group, and in pseudo-control group
respectively with a volume of 50 microliters of the SNPs solution (10 and 100
µg/ml), AgNO3solution (100 µg/ml), and distilled water was added to the sterile
bandage of mice, then the bandages were fixed in the shaved surface, but the
negative control group was without treatment and bandage. After 3 and 7 days,
histologic sampling of liver and kidney of mice were subsequently analysed.
Results: Histopathological studies demonstrated some changes such as dilatation of
the central vein, hyperemia, inflammatory cells in liver, and vacuolar
degeneration, cloudy swelling in kidney. Conclusion: These findings demonstrate
that SNPs (40 nm) can induce hepatotoxicity and nephrotoxicity in mice following
skin absorption in a dose-dependent manner. Therefore, it is necessary to measure
tissue levels of SNPs in all treatment mice. It is also recommended for further
research with various doses for different periods of time following various routes
of administration of SNPs. © 2018, Journal of Clinical and Diagnostic Research. All
rights reserved.
AU - Samani, P. Y.
AU - Arabi, M.
AU - Shadkhast, M.
AU - Pivaei, E.
DB - Scopus
DO - 10.7860/JCDR/2018/28535.11114
IS - 1
KW - Dermal toxicity
Hepatotoxicity
Nephrotoxicity
Silver nitrate
silver nanoparticle
silver nitrate
animal experiment
animal tissue
Article
controlled study
histopathology
hyperemia
inflammatory cell
liver toxicity
male
mouse
nephrotoxicity
nonhuman
practice guideline
skin absorption
M3 - Article
PY - 2018
SP - CC01-CC04
ST - Repeated-dose toxicity in mouse liver and kidney after skin exposure to
T2 - Journal of Clinical and Diagnostic Research
TI - Repeated-dose toxicity in mouse liver and kidney after skin exposure to
85041323005&doi=10.7860%2fJCDR
%2f2018%2f28535.11114&partnerID=40&md5=130a7c731168c447833924b8cc99b9e1
VL - 12
ID - 5485
ER -
TY - JOUR
AB - The new type of annulated imidazolium salt 1-naphthyl-2-pyridin-2-yl-2H-
imidazo[1,5-a] pyridin-4-ylium hexafluorophosphate (1.HPF6) and three novel N-
heterocyclic carbene complexes (NHCs) [Ag(1)(2)][PF6] (2), [Au(1)(2)][PF6] (3), and
[Au(1)Cl-3] 4 have been synthesized and characterized by different spectroscopic
techniques. The solid state structure of 2 has been determined by single crystal X-
ray diffraction studies. The complex 3 has been synthesized via trans-metallation,
whereas complex 4 was obtained via a disproportionation process. The cytotoxicities
of the complexes 2, 3 and 4 were tested in vitro against non-small lung carcinoma
(A549), colorectal carcinoma (HCT-116) and breast adenocarcinoma (MCF-7) cell
lines. The measured IC50 values showed that the Au (I) complex 3 is more potent
than the complexes 2 and 4 as well as cisplatin. (C) 2015 Elsevier B.V. All rights
reserved.
AN - WOS:000357996100026
AU - Samanta, T.
AU - Munda, R. N.
AU - Roymahapatra, G.
AU - Nandy, A.
AU - Das Saha, K.
AU - Al-Deyab, S. S.
AU - Dinda, J.
DA - AUG 15
DO - 10.1016/j.jorganchem.2015.05.049
PY - 2015
SN - 0022-328X
1872-8561
SP - 183-191
ST - Silver(I), Gold(I) and Gold(III)-N-Heterocyclic carbene complexes of naphthyl
substituted annelated ligand: Synthesis, structure and cytotoxicity
T2 - JOURNAL OF ORGANOMETALLIC CHEMISTRY
TI - Silver(I), Gold(I) and Gold(III)-N-Heterocyclic carbene complexes of naphthyl
substituted annelated ligand: Synthesis, structure and cytotoxicity
VL - 791
ID - 6476
ER -
TY - JOUR
AB - INTRODUCTION: Products using the antimicrobial properties of silver
nanoparticles (Ag-nps) may be found in health and consumer products that routinely
contact skin. OBJECTIVES: This study was designed to assess the potential
cytotoxicity of Ag-nps in human epidermal keratinocytes (HEKs) and their
inflammatory and penetrating potential into porcine skin in vivo. MATERIALS AND
METHODS: We used eight different Ag-nps in this study [unwashed/uncoated (20, 50,
and 80 nm particle diameter), washed/uncoated (20, 50, and 80 nm), and carbon-
coated (25 and 35 nm)]. Skin was dosed topically for 14 consecutive days. HEK
viability was assessed by MTT, alamarBlue (aB), and CellTiter 96 AQueous One
(96AQ). Release of the pro inflammatory mediators interleukin (IL)-1β, IL-6, IL-8,
IL-10, and tumor necrosis factor-α (TNF-α) were measured. RESULTS: The effect of
the unwashed Ag-nps on HEK viability after a 24-hr exposure indicated a significant
dose-dependent decrease (p < 0.05) at 0.34 μg/mL with aB and 96AQ and at 1.7 μg/mL
with MTT. However, both the washed Ag-nps and carbon-coated Ag-nps showed no
significant decrease in viability at any concentration assessed by any of the three
assays. For each of the unwashed Ag-nps, we noted a significant increase (p < 0.05)
in IL-1β, IL-6, IL-8, and TNF-α concentrations. We observed localization of all Ag-
nps in cyto plasmic vacuoles of HEKs. Macroscopic observations showed no gross
irritation in porcine skin, whereas microscopic and ultrastructural observations
showed areas of focal inflammation and localization of Ag-nps on the surface and in
the upper stratum corneum layers of the skin. CONCLUSION: This study provides a
better understanding Ag-nps safety in vitro as well as in vivo and a basis for
occupational and risk assessment. Ag-nps are non toxic when dosed in washed Ag-nps
solutions or carbon coated.
AU - Samberg, M. E.
AU - Oldenburg, S. J.
AU - Monteiro-Riviere, N. A.
DB - Scopus
DO - 10.1289/ehp.0901398
IS - 3
KW - Keratinocytes
Metal oxides
Nanoparticles
Nanotechnology
Penetration
Porcine skin
Silver
Skin
Toxicity
Animals
Cell Survival
Cells, Cultured
Evaluation Studies as Topic
Humans
Inflammation
Interleukins
Metal Nanoparticles
Particle Size
Swine
Time Factors
Sus
carbon
interleukin 1
interleukin 10
interleukin 1beta
interleukin 6
interleukin 8
nanoparticle
silver
article
cell vacuole
cell viability
controlled study
cytoplasm
cytotoxicity
dose response
evaluation
human
human cell
in vitro study
in vivo study
inflammation
keratinocyte
priority journal
risk assessment
skin
skin toxicity
statistical significance
stratum corneum
M3 - Article
PY - 2010
SP - 407-413
ST - Evaluation of silver nanoparticle toxicity in skin in vivo and keratinocytes
in vitro
T2 - Environmental Health Perspectives
TI - Evaluation of silver nanoparticle toxicity in skin in vivo and keratinocytes
in vitro
77649212361&doi=10.1289%2fehp.0901398&partnerID=40&md5=d0c427dcca73f3d43cf07d728c67
ce38
VL - 118
ID - 5753
ER -
TY - JOUR
AB - Diabetic wound infections and pressure ulcers pose a significant challenge to
healthcare providers worldwide. The current study provides new and innovative wound
care products that reduce inflammation, clear infection, and improve healing in an
animal model of pressure ulcers in diabetic rats. Ointment, hydrogel, and nanofiber
dressings were synthesized using 5% turmeric, 1% oregano, and 1% chitosan
nanoparticles and tested for antibacterial and cytotoxicity in vitro, and wound
healing effects in vivo. Turmeric ethanolic extract showed high antioxidant
activity compared to Oregano, Chitosan Nanoparticles, and Alginate silver (p-value
' 0.0001). The ointment and hydrogel formulation (5% Turmeric, 1% Oregano, and 1%
chitosan) showed lower cytotoxicity compared to the commercial Alginate silver
dressing. Ointment, hydrogel formulations, and commercial Alginate silver, showed
significant antibacterial activity with 100% efficacy on both Staphylococcus aureus
and Escherichia coli (p-value ' 0.0001), compared to nanofibers which showed 50%
reduction in bacterial growth (p-value ' 0.0001). The new formulations were tested
in a rat model of pressure ulcers. Ointment and nanofibers achieved complete wound
healing by day 15 compared to the hydrogel and commercial Alginate silver dressing,
which showed higher infection, and the wound remained partially open by day 21. In
conclusion, Turmeric, Oregano extracts, and chitosan nanoparticles can be used for
effective wound dressings in both diabetic and non-diabetic wounds. At relatively
low concentrations, this combination provides a promising new wound treatment
formulation that is antibacterial, anti-inflammatory, and antioxidant. © 2020
AU - Sami, D. G.
AU - Abdellatif, A.
AU - Azzazy, H. M. E.
DB - Scopus
DO - 10.1080/03639045.2020.1811305
IS - 10
KW - diabetic ulcer
nanomaterial
oregano
turmeric
wound dressing
Wound healing
Animals
Bandages
Curcuma
Origanum
Rats
Ulcer
alginic acid
essential oil
hydrogel
nanofiber
plant extract
antiinfective agent
animal experiment
animal model
animal tissue
Article
Asparagus racemosus
bacterial growth
cell structure
cell viability
comparative study
controlled study
cytotoxicity
diabetic foot
drug formulation
Escherichia coli
hydrodynamics
in vitro study
in vivo study
male
nonhuman
ointment
pathogenesis
pilot study
rat
rat model
skin
zeta potential
animal
bandage
ulcer
M3 - Article
PY - 2020
SP - 1613-1621
ST - Turmeric/oregano formulations for treatment of diabetic ulcer wounds
T2 - Drug Development and Industrial Pharmacy
TI - Turmeric/oregano formulations for treatment of diabetic ulcer wounds
85089970974&doi=10.1080%2f03639045.2020.1811305&partnerID=40&md5=96b50549ec41e82aa8
2a2ea91c2e0e7c
VL - 46
ID - 5279
ER -
TY - JOUR
AB - Background For years, silver has been used for the treatment of skin
injuries. Objective The effect of the oral administration of silver nanoparticles
on the wound healing process in male rats was studied. Materials and methods In
this experimental study, 30 Wistar male rats were randomly allocated to three
groups - the control and two silver nanoparticles treatment groups (30ppm and 60ppm
AgNPs concentration). In all rats, the full- thickness wound was induced under
general anesthesia. At 12 days post-wounding, microscopic evaluation of wound
healing - for example inflammatory cells, fibroblasts, angiogenesis and collagen
density - was completed. Results The percentage of wound healing between the
control and the treatment groups on the 12th day was significant (p<0.001).
Moreover, the number of inflammatory cells was significantly higher in the control
than in the treatment groups (p<0.001). The difference between the fibroblast
number and collagen density was more in the treatment groups than in the control
(p<0.001). Also, a considerable difference was observed between the number of
inflammatory cells and fibroblasts in the 60ppm compared to the 30ppm
concentration. Conclusion By inducing anti-inflammatory effects and increasing the
proliferation of fibroblasts and the expression of collagen, silver nanoparticles
at a concentration of 30ppm accelerated the wound healing process.
AN - WOS:000572173500002
AU - Samiee-Rad, F.
AU - Sofiabadi, M.
AU - Habibian, Z.
AU - Gheibi, N.
DA - MAR
DO - 10.33235/wpr.28.1.8-16
IS - 1
PY - 2020
SN - 1837-6304
2202-9729
SP - 8-16
ST - Effects of the oral administration of silver nanoparticles on wound healing
in male rats
T2 - WOUND PRACTICE AND RESEARCH
TI - Effects of the oral administration of silver nanoparticles on wound healing
in male rats
VL - 28
ID - 5896
ER -
TY - JOUR
AB - Objective: To evaluate the success and failure, apical sealing and
biocompatibility of silver amalgam, IRM (R), SuperEBA (R) and MTA as retrograde
filler materials. Study design: A metaanalysis is made of filler materials in
periapical surgery, evaluating a total of 30 articles published in recent years.
Results: Percentage success with silver amalgam was 76.5% and slightly inferior to
that afforded by IRM (R). Performance in turn increased considerably when the
materials used were SuperEBA (R) or MTA. As regards marginal leakage, MTA with a
mean leakage time of 65.5 days afforded the best results, followed by SuperEBA (R),
IRM (R) and silver amalgam. MTA was the most biocompatible of the materials
studied, with practically no inflammatory response, while inflammation proved mild
or moderate with SuperEBA (R), mild with IRM (R), and moderate to severe in the
case of silver amalgam. Tissue regeneration was only observed with MTA, in the same
way as cement appositioning. Bone neoformation was observed with all four filler
materials. Conclusions: MTA appears to be an ideal material, though the results
obtained require confirmation by in vivo studies.
AN - WOS:000259666300007
AU - Sanchez, A. F. Y.
AU - Berrocal, M. I. L.
AU - Gonzalez, J. M. M.
C7 - 10489957
DA - MAR
IS - 3
PY - 2008
SN - 1698-6946
SP - E180-E185
ST - Metaanalysis of filler materials in periapical surgery
T2 - MEDICINA ORAL PATOLOGIA ORAL Y CIRUGIA BUCAL
TI - Metaanalysis of filler materials in periapical surgery
VL - 13
ID - 6526
ER -
TY - JOUR
AB - This study sought to evaluate the in vitro biological response of human
gingival fibroblasts (HGFs) co-coltured with Streptococcus mitis to bisphenol A
glycidylmethacrylate/triethylene glycol dimethacrylate (BisGMA/TEGDMA) thermosets
coated with Chitlac-nAg, a nanocomposite system with antimicrobial properties. To
avoid bacterial adhesion to dental devices and to reduce cytotoxicity against
eukaryotic cells, we coated BisGMA/TEGDMA methacrylic thermosets with a new
material, Chitlac-nAg, formed by stabilizing silver nanoparticles, which have well-
known antimicrobial properties, with a polyelectrolyte solution containing Chitlac.
Cytotoxicity, cell morphology, cell migration and inflammatory interleukine-6 (IL-
6) and prostaglandin E 2 (PGE2) secretion were evaluated. Our results showed that
the cytotoxicity exerted on HGFs by our nanocomposite material was absent in our
co-culture model, where fibroblasts are able to adhere and migrate. After 24 h
thermosets coated with Chitlac as well as those coated with Chitlac-nAg exerted a
minimal cytotoxic effect on HGFs, while after 48 h LDH release rises up 20%.
Moreover the presence of S. mitis reduced this release in a greater amount with
Chitlac-nAg coated thermosets. The secretion of IL-6 was significant in both
Chitlac and Chitlac-nAg coated thermosets, but PGE2 production was minimal,
suggesting that the IL-6 production was not related to an inflammatory response.
Co-culture and the addiction of saliva did not influence IL-6 and PGE2 secretion.
Data obtained in the present work suggest that Chitlac n-Ag coated thermosets could
significantly improve the success rates of restorative dentistry, since they limit
bacterial adhesion and are not toxic to HGFs. © 2014 Sancilio et al.
AU - Sancilio, S.
AU - Di Giacomo, V.
AU - Di Giulio, M.
AU - Gallorini, M.
AU - Marsich, E.
AU - Travan, A.
AU - Tarusha, L.
AU - Cellini, L.
AU - Cataldi, A.
C7 - e96520
DB - Scopus
DO - 10.1371/journal.pone.0096520
IS - 5
KW - Anti-Infective Agents
Cell Movement
Cell Survival
Dinoprostone
Fibroblasts
Gingiva
Humans
Interleukin-6
Nanocomposites
Silver Compounds
Streptococcus mitis
bisphenol A bis(2 hydroxypropyl) ether dimethacrylate
interleukin 6
nanocomposite
prostaglandin E2
silver nanoparticle
triethylene glycol dimethacrylate
antiinfective agent
silver derivative
article
bacterium adherence
cell adhesion
cell migration
cell structure
coculture
cytokine release
cytotoxicity
fibroblast
fibroblast culture
gingiva
human
human cell
human tissue
inflammation
nonhuman
normal human
preventive dentistry
prostaglandin release
saliva
cell motion
cell survival
cytology
drug effects
metabolism
M3 - Article
PY - 2014
ST - Biological responses of Human Gingival Fibroblasts (HGFs) in an innovative
co-culture model with Streptococcus mitis to thermosets coated with a silver
polysaccharide antimicrobial system
T2 - PLoS ONE
TI - Biological responses of Human Gingival Fibroblasts (HGFs) in an innovative
co-culture model with Streptococcus mitis to thermosets coated with a silver
polysaccharide antimicrobial system
84900561345&doi=10.1371%2fjournal.pone.0096520&partnerID=40&md5=ee070e0c2f14725d053
1fecadf797783
VL - 9
ID - 5645
ER -
TY - JOUR
AB - Owing to the serious adverse effects caused by pyrimethamine and
sulfadiazine, the drugs commonly used to treat toxoplasmosis, there is a need for
treatment alternatives for this disease. Nanotechnology has enabled significant
advances toward this goal. This study was conducted to evaluate the activity of
biogenic silver nanoparticles (AgNp-Bio) in RAW 264.7 murine macrophages infected
with the RH strain of Toxoplasma gondii. The macrophages were infected with T.
gondii tachyzoites and then treated with various concentrations of AgNp-Bio. The
cells were evaluated by microscopy, and culture supernatants were collected for
ELISA determination of their cytokine concentration. Treatment with 6 mM AgNp-Bio
reduced the infection and parasite load in infected RAW 264.7 macrophages without
being toxic to the cells. The treatment also induced the synthesis of reactive
oxygen species and tumor necrosis factoralpha (both pro-inflammatory mediators),
which resulted in ultrastructural changes in the tachyzoites and their
intramacrophagic destruction. Our findings suggest that AgNp-Bio affect T. gondii
tachyzoites by activating microbicidal and pro-inflammatory mechanisms and may be a
potential alternative treatment for toxoplasmosis. (C) 2022 Institut Pasteur.
Published by Elsevier Masson SAS. All rights reserved.
AN - WOS:000861570500004
AU - Sanfelice, R. A. D.
AU - Silva, T. F.
AU - Bortoleti, B. T. D.
AU - Lazarin-Bidoia, D.
AU - Nakazato, G.
AU - Garcia, J. L.
AU - Verri, W. A.
AU - Costa, I. N.
C6 - JUL 2022
C7 - 104971
DA - JUL-AUG
DO - 10.1016/j.micinf.2022.104971
IS - 5
PY - 2022
SN - 1286-4579
1769-714X
ST - Biogenic silver nanoparticles reduce Toxoplasma gondii infection and
T2 - MICROBES AND INFECTION
TI - Biogenic silver nanoparticles reduce Toxoplasma gondii infection and
VL - 24
ID - 6002
ER -
TY - JOUR
AB - Knee osteoarthritis (KOA) is one of the most commonly encountered
degenerative diseases of the joints in people over 45 years of age. Currently,
there are not any effective therapeutics for KOA,and the only end-point strategy is
total knee arthroplasty (TKA); therefore, KOA is associated with economic burdens
and societal costs. The immune inflammatory response is involved in the occurrence
and development of KOA. We previously established a mouse model of KOA using type
II collagen. Hyperplasia of the synovial tissue was present in the model, alongside
a large number of infiltrated inflammatory cells. Silver nanoparticles have
substantial anti-inflammatory effects and have been widely used in tumor therapy
and surgical drug delivery. Therefore, we evaluated the therapeutic effects of
silver nanoparticles in a collagenase II-induced KOA model. The experimental
results showed that silver nanoparticles significantly reduced synovial hyperplasia
and the infiltration of neutrophils in the synovial tissue. Hence, this work
demonstrates the identification of a novel strategy for OA and provides a
theoretical basis for preventing the progress of KOA.
AN - WOS:001017787900040
AU - Sang, Y.
AU - Zhang, J. B.
AU - Liu, C.
AU - Liu, K. H.
AU - Yao, H.
AU - Zhao, H. Q.
AU - Xu, W. B.
AU - Xu, Y. C.
AU - Hou, G.
DA - JUN
DO - 10.3791/65111
IS - 196
PY - 2023
SN - 1940-087X
ST - Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
T2 - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
TI - Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
ID - 5951
ER -
TY - JOUR
AB - Metal and metal oxide nanoparticles are often used as industrial catalysts or
to improve product's functional properties. Recent advanced nanotechnology have
been expected to be used in various fields, ranging from sensors, environmental
remediation to biomedicine, medical biology and imaging, etc. However, the growing
use of nanoparticles has led to their release into environment and increased levels
of these particles at nearby sites or the surroundings of their manufacturing
factories become obvious. The toxicity of metal and metal oxide nanoparticles on
humans, animals, and certainly to the environment has become a major concern to our
community. However, controversies still remain with respect to the toxic effects
and the mechanisms of these nanoparticles. The scientific community now feels that
an understanding of the toxic effects is necessary to handle these nanoparticles
and their use. A new discipline, named nanotoxicology, has therefore been developed
that basically refers to the study of the interactions of nanoparticles with
biological systems and also measures the toxicity level related to human health.
Nanoparticles usually generate reactive oxygen species to a greater extent than
micro-sized particles resulting in increased pro-inflammatory reactions and
oxidative stress via intracellular signaling pathways. In this review, we mainly
focus on the routes of exposure of some metal and metal oxide nanoparticles and how
these nanoparticles affect us or broadly the cells of our organs. We would also
like to discuss the responsible mechanism(s) of the nanoparticle-induced reactive
oxygen species mediated organ pathophysiology. A brief introduction of the
characterization and application of these nanoparticles has also been included in
the article.
AN - WOS:000331803900045
AU - Sarkar, A.
AU - Ghosh, M.
AU - Sil, P. C.
DA - JAN
DO - 10.1166/jnn.2014.8752
IS - 1
PY - 2014
SN - 1533-4880
1533-4899
SP - 730-743
ST - Nanotoxicity: Oxidative Stress Mediated Toxicity of Metal and Metal Oxide
Nanoparticles
TI - Nanotoxicity: Oxidative Stress Mediated Toxicity of Metal and Metal Oxide
Nanoparticles
VL - 14
ID - 6393
ER -
TY - JOUR
AB - Modulation of pro-inflammatory and anti-inflammatory axis and orientation of
glial cell function towards neuroinflammation, were hallmark signs of cerebral
malaria (CM). CM pathogenesis was concerned with the circulating levels of
Interleukin 6 (IL 6) and Transforming growth factor β (TGF β). Definite roles of
these two cytokines in brain related pathology remained largely unexplored. To
study the effect of these two cytokines, we have examined changes in morphology and
in activation profile of the glial cells after TGF β and IL 6 neutralization during
CM in cortex and cerebellum of the Plasmodium berghei ANKA (PbA) infected male
swiss albino mice. PbA infection caused severe inflammation by inducing changes in
morphological features as well as in activation profile of the astrocytes and
microglia. Similar inflammatory signs were evident in Anti TGF β treated set.
Interestingly in the Anti IL 6 treated set, reduced level of activation of these
glial cells corresponds to the reduced level of inflammatory profile. Microglial
activation was found to be synchronous with TLR4 engagement. Neuronal death was
triggered by neuroinflammatory milieu seen in PbA and PbA + Anti TGF β treated set.
In conclusion, it can be said that IL 6 and TGF β perform essential role in CM
pathogenesis by modulating the level of glial cell induced neuroinflammation. ©
2017 Elsevier Ltd
AU - Sarkar, S.
AU - Keswani, T.
AU - Sengupta, A.
AU - Mitra, S.
AU - Bhattacharyya, A.
DB - Scopus
DO - 10.1016/j.cyto.2017.07.026
KW - Astrocyte
Interleukin 6
Microglia
Neuroinflammation
Plasmodium berghei ANKA
Transforming growth factor β
Animals
Apoptosis
Astrocytes
Biomarkers
Brain
CD11b Antigen
Cell Aggregation
Glial Fibrillary Acidic Protein
Inflammation
Interleukin-6
Malaria, Cerebral
Male
Mice
Neuroglia
Nuclear Proteins
Plasmodium berghei
Silver Staining
Transforming Growth Factor beta
CD11b antigen
interleukin 6
neuron specific nuclear protein
autacoid
biological marker
nerve protein
NeuN protein, mouse
nuclear protein
animal cell
animal experiment
animal model
animal tissue
Article
astrocyte
brain cortex
cell activation
cell structure
cerebellum
cerebral malaria
controlled study
glia cell
histopathology
immune response
immunofluorescence
inflammation
male
microglia
mouse
nerve cell necrosis
neuropathology
nonhuman
Plasmodium berghei ANKA infection
Plasmodium berghei infection
priority journal
silver staining
Swiss Webster mouse
synchronous culture
Western blotting
animal
apoptosis
brain
cell aggregation
glia
metabolism
pathology
physiology
M3 - Article
PY - 2017
SP - 249-259
ST - Differential modulation of glial cell mediated neuroinflammation in
Plasmodium berghei ANKA infection by TGF β and IL 6
T2 - Cytokine
TI - Differential modulation of glial cell mediated neuroinflammation in
Plasmodium berghei ANKA infection by TGF β and IL 6
85028363190&doi=10.1016%2fj.cyto.2017.07.026&partnerID=40&md5=35386de37a7e8524a945a
452134f8fd9
VL - 99
ID - 5547
ER -
TY - JOUR
AB - The NLRP3 inflammasome has been implicated in the pathogenesis of various
inflammatory diseases and is activated by particulate stimulants. Oral epithelial
keratinocytes are frequently exposed to metal nanoparticles. In this study, we
examined the effects of gold, silver, and palladium nanoparticles, which are
frequently used for dental metal alloys on cell proliferation, cytotoxicity,
autophagy, lysosomal functions, and NLRP3 inflammasome activation using the
immortalized human oral keratinocyte cell line RT-7. The metal nanoparticles were
agglomerated in the membrane vesicles in RT-7 cells and suppressed cell
proliferation and increased lactate dehydrogenase activity as well as the
proportion of apoptotic cells. Silver and palladium nanoparticles induced autophagy
and lysosomal dysfunctions and all metal nanoparticles tested triggered the
secretion of IL-1β through caspase-1 activation. Furthermore, the epithelium
obtained from patients with oral lichenoid lesions (OLLs) had robust NLRP3, ASC,
caspase-1, and IL-1β-positive keratinocytes and cDNA microarray showed significant
elevation in the mRNA levels of NLRP3. These results suggest that internalized
metal nanoparticles in oral mucosal epithelial cells activate the NLRP3
inflammasome through the induction of lysosomal damage and autophagy dysfunction.
This process may be involved in the pathogenesis of OLL and suggest its potential
as an alternative target for OLL therapy. © 2019 Elsevier Ltd
AU - Sasabe, E.
AU - Tomomura, A.
AU - Kitamura, N.
AU - Yamamoto, T.
C7 - 104663
DB - Scopus
DO - 10.1016/j.tiv.2019.104663
KW - Metal nanoparticles
NLRP3 inflammasome
Oral keratinocytes
Adolescent
Adult
Aged
Aged, 80 and over
Cell Line
Cell Proliferation
Cell Survival
Child
Female
Gold
Humans
Inflammasomes
Keratinocytes
Lichenoid Eruptions
Male
Metal Nanoparticles
Middle Aged
Mouth
Silver
Young Adult
complementary DNA
cryopyrin
gold nanoparticle
inflammasome
interleukin 1beta
messenger RNA
metal nanoparticle
palladium nanoparticle
silver nanoparticle
gold
NLRP3 protein, human
silver
adult
aged
apoptosis
Article
autophagy
cell damage
cell function
cell proliferation
clinical article
controlled study
DNA microarray
enzyme activation
enzyme activity
female
human
human cell
human tissue
keratinocyte
keratinocyte cell line
lichenoid
lysosome
male
membrane vesicle
oral lichenoid lesion
pathogenesis
protein function
protein secretion
RT-7 cell line
adolescent
cell line
cell survival
child
cytology
drug effect
lichenoid eruption
metabolism
middle aged
mouth
very elderly
young adult
M3 - Article
PY - 2020
ST - Metal nanoparticles-induced activation of NLRP3 inflammasome in human oral
keratinocytes is a possible mechanism of oral lichenoid lesions
TI - Metal nanoparticles-induced activation of NLRP3 inflammasome in human oral
keratinocytes is a possible mechanism of oral lichenoid lesions
85074284107&doi=10.1016%2fj.tiv.2019.104663&partnerID=40&md5=91299186c92e1852bbd42d
c484000297
VL - 62
ID - 5268
ER -
TY - JOUR
AB - We have previously shown that TNF-tumor necrosis factor receptor-2/p75
(TNFR2/p75) signaling plays a critical role in ischemia-induced neovascularization
in skeletal muscle and heart tissues. To determine the role of TNF-TNFR2/p75
signaling in ischemia-induced inflammation and muscle regeneration, we subjected
wildtype (WT) and TNFR2/p75 knockout (p75KO) mice to hind limb ischemia (HLI)
surgery. Ischemia induced significant and long-lasting inflammation associated with
considerable decrease in satellite-cell activation in p75KO muscle tissue up to 10
d after HLI surgery. To determine the possible additive negative roles of tissue
aging and the absence of TNFR2/p75, either in the tissue or in the bone marrow(BM),
we generated 2 chimeric BM transplantation (BMT) models where both young green
fluorescent protein (GFP)-positive p75KO and WT BM-derived cells were transplanted
into adult p75KO mice. HLI surgery was performed 1 mo after BMT, after confirming
complete engraftment of the recipient BM with GFP donor cells. In adult p75KO with
the WT-BMT, proliferative (Ki67(+)) cells were detected only by d 28 and were
exclusively GFP(+), suggesting significantly delayed contribution of young WT-BM
cell to adult p75KO ischemic tissue recovery. No GFP(+) young p75KO BM cells
survived in adult p75KO tissue, signifying the additive negative roles of tissue
aging combined with decreased/absent TNFR2/p75 signaling in postischemic recovery.
AN - WOS:000354115000010
AU - Sasi, S. P.
AU - Rahimi, L.
AU - Yan, X. H.
AU - Silver, M.
AU - Qin, G. J.
AU - Losordo, D. W.
AU - Kishore, R.
AU - Goukassian, D. A.
DA - APR
DO - 10.1096/fj.14-249813
IS - 4
PY - 2015
SN - 0892-6638
1530-6860
SP - 1208-1219
ST - Genetic deletion of TNFR2 augments inflammatory response and blunts
satellite-cell-mediated recovery response in a hind limb ischemia model
T2 - FASEB JOURNAL
TI - Genetic deletion of TNFR2 augments inflammatory response and blunts
satellite-cell-mediated recovery response in a hind limb ischemia model
VL - 29
ID - 6427
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are incorporated into medical devices for their
anti-microbial characteristics. The potential exposure and toxicity of AgNPs is
unknown due to varying physicochemical particle properties and lack of
toxicological data. The aim of this safety assessment is to derive a provisional
tolerable intake (pTI) value for AgNPs released from blood-contacting medical
devices. A literature review of in vivo studies investigating critical health
effects induced from intravenous (i.v.) exposure to AgNPs was evaluated by the
Annapolis Accords principles and Toxicological Data Reliability Assessment Tool
(ToxRTool). The point of departure (POD) was based on an i.v. 28-day repeated AgNP
(20 nm) dose toxicity study reporting an increase in relative spleen weight in rats
with a 5% lower confidence bound of the benchmark dose (BMDL05) of 0.14 mg/kg
bw/day. The POD was extrapolated to humans by a modifying factor of 1,000 to
account for intraspecies variability, interspecies differences and lack of long-
term toxicity data. The pTI for long-term i.v. exposure to 20 nm AgNPs released
from blood-contacting medical devices was 0.14 pg/kg bw/day. This pTI may not be
appropriate for nanoparticles of other physicochemical properties or routes of
administration. The methodology is appropriate for deriving pTls for nanoparticles
in general. Published by Elsevier Inc.
AN - WOS:000396968500012
AU - Savery, L. C.
AU - Vinas, R.
AU - Nagy, A. M.
AU - Pradeep, P.
AU - Merrill, S. J.
AU - Hood, A. M.
AU - Malghan, S. G.
AU - Goering, P. L.
AU - Brown, R. P.
DA - APR
DO - 10.1016/j.yrtph.2017.01.007
PY - 2017
SN - 0273-2300
1096-0295
SP - 108-118
ST - Deriving a provisional tolerable intake for intravenous exposure to silver
nanoparticles released from medical devices
T2 - REGULATORY TOXICOLOGY AND PHARMACOLOGY
TI - Deriving a provisional tolerable intake for intravenous exposure to silver
nanoparticles released from medical devices
VL - 85
ID - 6052
ER -
TY - JOUR
AB - Currently available murine staphylococcal enterotoxin B (SEB) shock models
require pretreatment with various agents to increase mouse sensitivity to SEB. This
study was performed to show that C3H/HeJ mice are highly susceptible to intranasal
SEB inoculation, which caused toxic shock without using pretreatment agents. For
this purpose, mice were injected intranasally with different doses of SEB and
observed for up to 1 month. The median lethal dose of SEB was determined using the
probit procedure. Tissue samples were taken at different time points for
histopathological examination. The LD50 was found at 1.6 mug/g (95% fiducial limit
(f.l.) 0.7 to 2.2), the LD80 at 2.7 mug/g (95% f.l. 1.9 to 4.0) and the LD90 at 3.6
mug/g (95% f.l. 2.7 to 6.4). Histopathologic examination revealed pulmonary edema
and bronchopneumonia. Mucosal-associated lymphoid tissue first became activated,
followed by increasing lymphocyte apoptosis and depletion. In the liver there were
intralobular and portal inflammatory foci with increasing lymphocyte apoptosis and
degenerative necrosis. The splenic white pulp was characterized by early activation
and subsequent depletion of lymphoid follicle germinal centers. The thymus
initially was activated, followed by increasing apoptosis and migration of lymphoid
cells from the cortex to the medulla. The pathological features detected in the
mice were similar to those of rhesus monkeys treated with SEB aerosol challenge.
AN - WOS:000184788800003
AU - Savransky, V.
AU - Rostapshov, V.
AU - Pinelis, D.
AU - Polotsky, Y.
AU - Korolev, S.
AU - Komisar, J.
AU - Fegeding, K.
DA - JUL-AUG
DO - 10.1080/01926230390201093
IS - 4
PY - 2003
SN - 0192-6233
SP - 373-378
ST - Murine lethal toxic shock caused by intranasal administration of
staphylococcal enterotoxin B
T2 - TOXICOLOGIC PATHOLOGY
TI - Murine lethal toxic shock caused by intranasal administration of
staphylococcal enterotoxin B
VL - 31
ID - 6680
ER -
TY - JOUR
AB - Accumulation of formaldehyde (FA) in the brain is linked to age-related
neurodegenerative disorders, as it accelerates memory impairment through tau
protein aggregation, inflammation, and nuclear damage. This study aimed to assess
the possible effects of methanolic cinnamon extract (CE) on FA-induced
neurotoxicity in rats. The animals were treated with CE (100, 200, and 400 mg/kg,
P.O.) for 30 days following FA administration (60 mg/kg, I.P.) for 30 days.
Briefly, spatial and inhibitory memory were examined by Morris water maze (MWM) and
passive avoidance (PA) tasks, respectively. The Niss1, Hoechst, and Bielschowsky
silver staining methods were also used to assess apoptosis and neurofibrillary
tangles (NFTs) in the hippocampal CA1 region, respectively. Brain tissues were
probed with an anti-phospho-tau (Thr(231)) monoclonal antibody to assess tau
hyperphosphorylation. Inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) were
also measured by ELISA assay. Western blotting was performed to quantify the amount
of phospho-tau (Thr(231)), caspase-8, and caspase-9. The results showed that FA
injection significantly caused tau hyperphosphorylation at Thr(231) residue, which
in turn disturbed the MWM performance. The ratio of apoptotic to intact neurons
increased following FA treatment. The results of Western blotting indicated that
the hippocampal levels of phospho-tau (Thr(231)) and caspase-8 were significantly
higher in the FA group compared to the control group. The hippocampal levels of IL-
1 beta, IL-6, and TNF-alpha in the FA group were also higher than the control group
Administration of 200 mg/kg of CE significantly improved the rats' MWM performance,
decreased the levels of phospho-tau (Thr(231)), caspase-8, IL-6, and TNF-alpha, and
reduced the ratio of apoptotic to intact neurons. Overall, cinnamon improved
cognitive performance in FA-treated rats by eliminating tau hyperphosphorylation,
inflammatory cytokines, and nuclear damage.
AN - WOS:000535788200001
AU - Sayad-Fathi, S.
AU - Zaminy, A.
AU - Babaei, P.
AU - Yousefbeyk, F.
AU - Azizi, N. E.
AU - Nasiri, E.
DO - 10.17179/excli2020-1960
PY - 2020
SN - 1611-2156
SP - 671-686
ST - THE METHANOLIC EXTRACT OF CINNAMOMUM ZEYLANICUM BARK IMPROVES FORMALDEHYDE-
INDUCED NEUROTOXICITY THROUGH REDUCTION OF PHOSPHO-TAU (THR231), INFLAMMATION, AND
APOPTOSIS
T2 - EXCLI JOURNAL
TI - THE METHANOLIC EXTRACT OF CINNAMOMUM ZEYLANICUM BARK IMPROVES FORMALDEHYDE-
INDUCED NEUROTOXICITY THROUGH REDUCTION OF PHOSPHO-TAU (THR231), INFLAMMATION, AND
APOPTOSIS
VL - 19
ID - 6600
ER -
TY - JOUR
AB - Accumulation of formaldehyde (FA) in the brain is linked to age-related
neurodegenerative disorders, as it accel-erates memory impairment through tau
protein aggregation, inflammation, and nuclear damage. This study aimed to assess
the possible effects of methanolic cinnamon extract (CE) on FA-induced
neurotoxicity in rats. The animals were treated with CE (100, 200, and 400 mg/kg,
P.O.) for 30 days following FA administration (60 mg/kg, I.P.) for 30 days.
Briefly, spatial and inhibitory memory were examined by Morris water maze (MWM) and
passive avoidance (PA) tasks, respectively. The Nissl, Hoechst, and Bielschowsky
silver staining methods were also used to assess apoptosis and neurofibrillary
tangles (NFTs) in the hippocampal CA1 region, respectively. Brain tissues were
probed with an anti-phospho-tau (Thr231) monoclonal antibody to assess tau
hyperphosphorylation. Inflammatory cytokines (IL-1β, IL-6, and TNF-α) were also
measured by ELISA assay. Western blotting was performed to quantify the amount of
phospho-tau (Thr231), caspase-8, and caspase-9. The results showed that FA
injection significantly caused tau hyperphosphorylation at Thr231 residue, which in
turn disturbed the MWM performance. The ratio of apoptotic to intact neurons
increased following FA treatment. The results of Western blotting indicated that
the hippocampal levels of phospho-tau (Thr231) and caspase-8 were significantly
higher in the FA group com-pared to the control group. The hippocampal levels of
IL-1β, IL-6, and TNF-α in the FA group were also higher than the control group.
Administration of 200 mg/kg of CE significantly improved the rats’ MWM performance,
decreased the levels of phospho-tau (Thr231), caspase-8, IL-6, and TNF-α, and
reduced the ratio of apoptotic to intact neurons. Overall, cinnamon improved
cognitive performance in FA-treated rats by eliminating tau hyper-phosphorylation,
inflammatory cytokines, and nuclear damage. © 2020, Leibniz Research Centre for
Working Environment and Human Factors. All rights reserved.
AU - Sayad-Fathi, S.
AU - Zaminy, A.
AU - Babaei, P.
AU - Yousefbeyk, F.
AU - Azizi, N.
AU - Nasiri, E.
DB - Scopus
DO - 10.17179/excli2020-1960
KW - Cinnamomum zeylanicum
Formaldehyde
Neurotoxicity
Spatial memory
Tauopathy
caspase 8
caspase 9
cinnamon extract
formaldehyde
interleukin 1beta
interleukin 6
methanol
phenol derivative
tau protein
animal experiment
animal model
animal tissue
apoptosis
Article
controlled study
drug mechanism
inflammation
male
mental performance
Morris water maze test
neurofibrillary tangle
neurotoxicity
nonhuman
passive avoidance test
rat
spatial memory
Western blotting
M3 - Article
PY - 2020
SP - 671-686
ST - The methanolic extract of cinnamomum zeylanicum bark improves formaldehyde-
induced neurotoxicity through reduction of phospho-tau (Thr231), inflammation, and
apoptosis
T2 - EXCLI Journal
TI - The methanolic extract of cinnamomum zeylanicum bark improves formaldehyde-
induced neurotoxicity through reduction of phospho-tau (Thr231), inflammation, and
apoptosis
85085886693&doi=10.17179%2fexcli2020-
1960&partnerID=40&md5=9e587ee7e70ad627050d49a015afd86c
VL - 19
ID - 5398
ER -
TY - JOUR
AB - Background: The objective of this study was to examine the threshold fibre
length for the onset of pulmonary inflammation after aspiration exposure in mice to
four different lengths of silver nanowires (AgNW). We further examined the effect
of fibre length on macrophage locomotion in an in vitro wound healing assay. We
hypothesised that exposure to longer fibres causes both increased inflammation and
restricted mobility leading to impaired clearance of long fibres from the lower
respiratory tract to the mucociliary escalator in vivo.Methods: Nine week old
female C57BL/6 strain mice were exposed to AgNW and controls via pharyngeal
aspiration. The dose used in this study was equalised to fibre number and based on
50 μg/ mouse for AgNW14. To examine macrophage migration in vitro a wound healing
assay was used. An artificial wound was created in a confluent layer of bone marrow
derived macrophages by scraping with a pipette tip and the number of cells
migrating into the wound was monitored microscopically. The dose was equalised for
fibre number and based on 2.5 μg/cm2 for AgNW14.. Results: Aspiration of AgNW
resulted in a length dependent inflammatory response in the lungs with threshold at
a fibre length of 14 μm. Shorter fibres including 3, 5 and 10 μm elicited no
significant inflammation. Macrophage locomotion was also restricted in a length
dependent manner whereby AgNW in the length of ≥5 μm resulted in impaired motility
in the wound closure assay.Conclusion: We demonstrated a 14 μm cut-off length for
fibre-induced pulmonary inflammation after aspiration exposure and an in vitro
threshold for inhibition of macrophage locomotion of 5 μm. We previously reported a
threshold length of 5 μm for fibre-induced pleural inflammation. This difference in
pulmonary and pleural fibre- induced inflammation may be explained by differences
in clearance mechanism of deposited fibres from the airspaces compared to the
pleural space. Inhibition of macrophage migration at long fibre lengths could
account for their well-documented long term retention in the lungs compared to
short fibres. Knowledge of the threshold length for acute pulmonary inflammation
contributes to hazard identification of nanofibres. © 2012 Schinwald et al.;
AU - Schinwald, A.
AU - Chernova, T.
AU - Donaldson, K.
C7 - 47
DB - Scopus
DO - 10.1186/1743-8977-9-47
KW - Asbestos fibre
Aspiration
Clearance
Migration
Silver nanofibres
Animals
Cell Movement
Cells, Cultured
Female
Mice
Mice, Inbred C57BL
Nanowires
Particle Size
Phagocytosis
Pneumonia
Silver
Surface Properties
Mus
glycogen synthase kinase 3alpha
glycogen synthase kinase 3beta
nanowire
protein kinase B
silver nanowire
unclassified drug
animal cell
animal experiment
animal model
animal tissue
article
controlled study
enzyme activation
female
fiber
histopathology
in vitro study
in vivo study
lung clearance
lung granuloma
lung lavage
lung toxicity
macrophage migration inhibition
mouse
mucociliary clearance
nanofabrication
nonhuman
particle size
phagocytosis
pneumonia
priority journal
pulmonary aspiration
toxic inhalation
wound healing
M3 - Article
PY - 2012
ST - Use of silver nanowires to determine thresholds for fibre length-dependent
pulmonary inflammation and inhibition of macrophage migration in vitro
TI - Use of silver nanowires to determine thresholds for fibre length-dependent
pulmonary inflammation and inhibition of macrophage migration in vitro
84870182326&doi=10.1186%2f1743-8977-9-
47&partnerID=40&md5=1d7469336c11fd6f97813920bb5cf949
VL - 9
ID - 5751
ER -
TY - JOUR
AB - Suspicion has been raised that high aspect ratio nanoparticles or nanofibers
might possess asbestos-like pathogenicity. The pleural space is a specific target
for disease in individuals exposed to asbestos and by implication of nanofibers.
Pleural effects of fibers depends on fiber length, but the key threshold length
beyond which adverse effects occur has never been identified till now because all
asbestos and vitreous fiber samples are heterogeneously distributed in their
length. Nanotechnology advantageously allows for highly defined length distribution
of synthetically engineered fibers that enable for in-depth investigation of this
threshold length. We utilized the ability to prepare silver nanofibers of five
defined length classes to demonstrate a threshold fiber length for acute pleural
inflammation. Nickel nanofibers and carbon nanotubes were then used to strengthen
the relationship between fiber length and pleural inflammation. A method of
intrapleural injection of nanofibers in female C57Bl/6 strain mice was used to
deliver the fiber dose, and we then assessed the acute pleural inflammatory
response. Chest wall sections were examined by light and scanning electron
microscopy to identify areas of lesion; furthermore, cell-nanowires interaction on
the mesothelial surface of the parietal pleura in vivo was investigated. Our
results showed a clear threshold effect, demonstrating that fibers beyond 4 μm in
length are pathogenic to the pleura. The identification of the threshold length for
nanofiber-induced pathogenicity in the pleura has important implications for
understanding the structure-toxicity relationship for asbestos-induced mesothelioma
and consequent risk assessment with the aim to contribute to the engineering of
synthetic nanofibers by the adoption of a benign-by-design approach. © The Author
2012. Published by Oxford University Press on behalf of the Society of Toxicology.
AU - Schinwald, A.
AU - Murphy, F. A.
AU - Prina-Mello, A.
AU - Poland, C. A.
AU - Byrne, F.
AU - Movia, D.
AU - Glass, J. R.
AU - Dickerson, J. C.
AU - Schultz, D. A.
AU - Jeffree, C. E.
AU - Macnee, W.
AU - Donaldson, K.
DB - Scopus
DO - 10.1093/toxsci/kfs171
IS - 2
KW - Asbestos
Fiber threshold
HARN
Pleural inflammation
Risk assessment
Silver nanowires
Animals
Female
Mesothelioma
Metals
Mice
Mice, Inbred C57BL
Nanofibers
Phagocytosis
Pleurisy
asbestos
carbon nanotube
nanofiber
nanoparticle
nanowire
nickel nanofiber
nickel nanowire
silver nanofiber
silver nanoparticle
silver nanowire
unclassified drug
animal experiment
animal model
animal tissue
article
cell interaction
cell phagocytosis
controlled study
disease course
female
in vivo study
mouse
nanotechnology
nonhuman
particle size
pathogenicity
pleura cavity
pleura mesothelioma
pleurisy
risk assessment
thorax wall
M3 - Article
PY - 2012
SP - 461-470
ST - The threshold length for fiber-induced acute pleural inflammation: Shedding
light on the early events in asbestos-induced mesothelioma
TI - The threshold length for fiber-induced acute pleural inflammation: Shedding
light on the early events in asbestos-induced mesothelioma
84865349541&doi=10.1093%2ftoxsci
%2fkfs171&partnerID=40&md5=c3d3ee89db6ebb3b3f1b71b2c8950d59
VL - 128
ID - 5683
ER -
TY - JOUR
AB - OBJECTIVES: Bovine and porcine pericardial patches are frequently used in
cardiothoracic and vascular surgery. There are no guidelines recommending the usage
of these patches for particular surgical approaches. However, these 2 materials
supposedly possess different properties. The clinical advantage of porcine compared
with bovine patches remains controversial. In this experimental study, we analysed
the incorporation and vascularization of bovine and porcine pericardial patches
during the initial phase after implantation. METHODS: Bovine and porcine
pericardial patches were implanted into the dorsal skinfold chamber of C57BL/6 mice
(n = 8 per group) to study vascularization and inflammation at the implantation
site using repetitive intravital fluorescence microscopy over a 14-day period. At
the end of the in vivo experiments, CD-31-positive cells were determined to
evaluate the vascularization by immunohistochemistry. Furthermore, cell
proliferation and apoptosis were analysed immunohistochemically. RESULTS: Implanted
bovine patches exhibited an enhanced vascularization, as indicated by a
significantly higher number of CD-31-positive cells and micro-vessels (23.2 +/- 4.3
vs 16.5 +/- 5.8 mm(-2); P=0.001). Furthermore, bovine patches showed a slightly but
not significantly higher functional capillary density. Both patches induced a
moderate leukocytic inflammatory host tissue response, and neither bovine nor
porcine patches significantly affected apoptosis and cell proliferation at the
implantation site. CONCLUSIONS: Bovine and porcine pericardial patches are
similarly suitable for surgery. Bovine patches exhibited an improved
vascularization during the first 14 days after implantation. This may result in a
quicker and improved incorporation into the surrounding tissue compared with
porcine pericardial patches.
AN - WOS:000648883600020
AU - Schlachtenberger, G.
AU - Doerr, F.
AU - Brezina, A.
AU - Menghesha, H.
AU - Heldwein, M. B.
AU - Bennink, G.
AU - Menger, M. D.
AU - Moussavian, M.
AU - Hekmat, K.
AU - Wahlers, T.
DA - APR
DO - 10.1093/icvts/ivaa308
IS - 4
PY - 2021
SN - 1569-9293
1569-9285
SP - 638-647
ST - Perigraft reaction and incorporation of porcine and bovine pericardial
patches
T2 - INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY
TI - Perigraft reaction and incorporation of porcine and bovine pericardial
patches
VL - 32
ID - 6730
ER -
TY - JOUR
AB - Nanoparticles (NPs) can potentially cause adverse effects on organ, tissue,
cellular, subcellular, and protein levels due to their unusual physicochemical
properties (e.g., small size, high surface area to volume ratio, chemical
composition, crystallinity, electronic properties, surface structure reactivity and
functional groups, inorganic or organic coatings, solubility, shape, and
aggregation behavior). Metal NPs, in particular, have received increasing interest
due to their widespread medical, consumer, industrial, and military applications.
However, as particle size decreases, some metal-based NPs are showing increased
toxicity, even if the same material is relatively inert in its, bulk form (e.g.,
Ag, Au, and Cu). NPs also interact with proteins and enzymes within mammalian cells
and they can interfere with the antioxidant defense mechanism leading to reactive
oxygen species generation, the initiation of an inflammatory response and
perturbation and destruction of the mitochondria causing apoptosis or necrosis. As
a result, there are many challenges to overcome before we can determine if the
benefits outweigh the risks associated with NPs. (C) 2010 John Wiley & Sons, Inc.
WIREs Nanomed Nanobiotechnol 2010 2 544-568
AN - WOS:000281456300008
AU - Schrand, A. M.
AU - Rahman, M. F.
AU - Hussain, S. M.
AU - Smith, D. A.
AU - Ali, S. F.
DA - SEP-OCT
DO - 10.1002/wnan.103
IS - 5
PY - 2010
SN - 1939-5116
1939-0041
SP - 544-568
ST - Metal-based nanoparticles and their toxicity assessment
T2 - WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
TI - Metal-based nanoparticles and their toxicity assessment
VL - 2
ID - 6365
ER -
TY - JOUR
AB - Nanoparticles (NPs) can potentially cause adverse effects on organ, tissue,
cellular, subcellular, and protein levels due to their unusual physicochemical
properties (e.g., small size, high surface area to volume ratio, chemical
composition, crystallinity, electronic properties, surface structure reactivity and
functional groups, inorganic or organic coatings, solubility, shape, and
aggregation behavior). Metal NPs, in particular, have received increasing interest
due to their widespread medical, consumer, industrial, and military applications.
However, as particle size decreases, some metal-based NPs are showing increased
toxicity, even if the same material is relatively inert in its bulk form (e.g., Ag,
Au, and Cu). NPs also interact with proteins and enzymes within mammalian cells and
they can interfere with the antioxidant defense mechanism leading to reactive
oxygen species generation, the initiation of an inflammatory response and
perturbation and destruction of the mitochondria causing apoptosis or necrosis. As
a result, there are many challenges to overcome before we can determine if the
benefits outweigh the risks associated with NPs. © 2010 John Wiley & Sons, Inc.
AU - Schrand, A. M.
AU - Rahman, M. F.
AU - Hussain, S. M.
AU - Smith, D. A.
AU - Syed, A. F.
DB - Scopus
DO - 10.1002/wnan.103
IS - 5
KW - Animals
Cerium
Humans
Metal Nanoparticles
Metals, Heavy
Particle Size
Silicon Dioxide
Toxicity Tests
Agglomeration
Cell death
Electronic properties
Functional groups
Inorganic coatings
Mammals
Military applications
Nanoparticles
Organic coatings
Oxygen
Silver
Surface structure
Toxicity
aluminum
cerium oxide
copper
gold
iron oxide
lead
manganese oxide
metal nanoparticle
nickel
silicon dioxide
silver
titanium dioxide
zinc oxide
Adverse effect
Aggregation behavior
Antioxidant defense
Apoptosis
Chemical compositions
Crystallinities
High surface area
Mammalian cells
Protein level
Small size
Structure reactivity
Sub-cellular
Toxicity assessment
Volume ratio
apoptosis
biomedical engineering
cell destruction
cell viability
defense mechanism
human
inflammation
nonhuman
phagocytosis
priority journal
protein interaction
review
Surface properties
M3 - Review
PY - 2010
SP - 544-568
ST - Metal-based nanoparticles and their toxicity assessment
TI - Metal-based nanoparticles and their toxicity assessment
78449296021&doi=10.1002%2fwnan.103&partnerID=40&md5=addeb6be2ba7f76b9057a01f9001e78
0
VL - 2
ID - 5662
ER -
TY - JOUR
AB - Under natural conditions and in some experimental models, rabies virus
infection of the central nervous system causes relatively mild histopathological
changes, without prominent evidence of neuronal death despite its lethality. In
this study, the effects of rabies virus infection on the structure of neurons were
investigated with experimentally infected transgenic mice expressing yellow
fluorescent protein (YFP) in neuronal subpopulations. Six-week-old mice were
inoculated in the hind-limb footpad with the CVS strain of fixed virus or were mock
infected with vehicle (phosphate-buffered saline). Brain regions were subsequently
examined by light, epifluorescent, and electron microscopy. In moribund CVS-
infected mice, histopathological changes were minimal in paraffin-embedded tissue
sections, although mild inflammatory changes were present. Terminal
deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling and caspase-3
immunostaining showed only a few apoptotic cells in the cerebral cortex and
hippocampus. Silver staining demonstrated the preservation of cytoskeletal
integrity in the cerebral cortex. However, fluorescence microscopy revealed marked
beading and fragmentation of the dendrites and axons of layer V pyramidal neurons
in the cerebral cortex, cerebellar mossy fibers, and axons in brainstem tracts. At
an earlier time point, when mice displayed hind-limb paralysis, beading was
observed in a few axons in the cerebellar commissure. Toluidine blue-stained
resinembedded sections from moribund YFP-expressing animals revealed vacuoles
within the perikarya and proximal dendrites of pyramidal neurons in the cerebral
cortex and hippocampus. These vacuoles corresponded with swollen mitochondria under
electron microscopy. Vacuolation was also observed ultrastructurally in axons and
in presynaptic nerve endings. We conclude that the observed structural changes are
sufficient to explain the severe clinical disease with a fatal outcome in this
experimental model of rabies. Copyright © 2008, American Society for Microbiology.
All Rights Reserved.
AU - Scott, C. A.
AU - Rossiter, J. P.
AU - Andrew, R. D.
AU - Jackson, A. C.
DB - Scopus
DO - 10.1128/JVI.01677-07
IS - 1
KW - Animals
Apoptosis
Axons
Brain
Brain Stem
Cerebellum
Cerebral Cortex
Cytoskeleton
Dendrites
Female
Inflammation
Luminescent Proteins
Male
Mice
Mice, Transgenic
Mitochondria
Nerve Fibers
Neurons
Pyramidal Cells
Rabies
Rabies virus
Vacuoles
Animalia
Mus
Mus musculus
adenosine triphosphatase (potassium sodium)
caspase 3
tolonium chloride
voltage gated sodium channel
yellow fluorescent protein
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
brain cortex
cell structure
cell vacuole
central nervous system infection
controlled study
dendrite
electron microscopy
fatality
foot pad
histopathology
mouse
nerve cell
nerve fiber
nick end labeling
nonhuman
perikaryon
priority journal
protein expression
pyramidal nerve cell
transgenic mouse
treatment outcome
M3 - Article
PY - 2008
SP - 513-521
ST - Structural abnormalities in neurons are sufficient to explain the clinical
disease and fatal outcome of experimental rabies in yellow fluorescent protein-
expressing transgenic mice
T2 - Journal of Virology
TI - Structural abnormalities in neurons are sufficient to explain the clinical
disease and fatal outcome of experimental rabies in yellow fluorescent protein-
expressing transgenic mice
37349019017&doi=10.1128%2fJVI.01677-
07&partnerID=40&md5=114c78fe9b82a184a2d163b6babfa74c
VL - 82
ID - 5786
ER -
TY - JOUR
AB - The aim of this project was the histopathological and immunofluorescence
evaluation of the cutaneous toxicity of the vesicant chemical compound 2-
chloroethyl-ethyl sulphide (CEES), a yperite simulator, and the effectiveness of
the applied antidote. A complex antidote formula was developed, the treatment
offered 100% protection in case of exposure to 1DL50 chemical vesicant. The
histopathological evaluation showed that the association of the newly developed
antidote with antioxidant and anti-inflammatory actions with the antidotic
formulation, in the form of gel, with regenerating, moisturizing and
epithelializing actions, is beneficial for 0.25-1 LD50 2-chloroethyl-ethyl sulphide
concentrations. Immunofluorescence evaluation of the expression of anti-c-ROS-1 and
anti-PARP-1 antibodies, respectively, highlighted the antidysplastic
reepithelialising and nuclear stabilizing protective effect of the complex antidote
on the skin lesions induced by the studied vesicant compound. The combination of
the two complex curative antidotes (A1 formulated as a solution and A2 formulated
as a hydrogel) developed within the project has superior therapeutic efficacy. It
can guide the therapeutic conduct in case of exposure to vesicant chemicals. ©
2022, Romanian Society for Pharmaceutical Sciences. All rights reserved.
AU - Secară, C. A.
AU - Catrina, A. M.
AU - Voinea, O. C.
AU - Dinu, S. S.
AU - Hîrjău, A. C.
AU - Șerbănescu, L. G.
AU - Serban, D.
AU - Smarandache, G. C.
AU - Haidoiu, C.
AU - Radu, S.
AU - Tudor, C.
AU - Costea, D. O.
AU - Comandasu, M.
AU - Dascalu, A. M.
AU - Păuna, A.
AU - Tudosie, M. S.
DB - Scopus
DO - 10.31925/farmacia.2022.3.11
IS - 3
KW - 2-chlorethyl-ethyl sulphide
antidote
CEES
toxicity
vesicants
2 chloroethyl ethyl sulphide
acetylcysteine
collagen
dexamethasone
disodium hydrogen phosphate
doxycycline
hyaluronic acid
hydrogel
lanolin
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1
nicotinamide nucleotide
potassium chloride
potassium dihydrogen phosphate
sodium chloride
sulfadiazine silver
toxic substance
unclassified drug
acanthosis
animal experiment
animal model
animal tissue
Article
blister
chemical burn
confocal microscopy
controlled study
drug dosage form
drug efficacy
drug formulation
epithelization
female
histopathology
hyperkeratosis
immunofluorescence
in vivo study
LD50
male
nonhuman
protein expression
skin protection
skin toxicity
M3 - Article
PY - 2022
SP - 456-464
ST - IN VIVO ASSESSMENT OF SKIN CHEMICAL BURNS IN EXPOSURE TO VESICANTS AND THE
EFFICACY OF AN ANTIDOTE FORMULA IN DIFFERENT PHARMACEUTICAL FORMS. AN EXPERIMENTAL
APPROACH
T2 - Farmacia
TI - IN VIVO ASSESSMENT OF SKIN CHEMICAL BURNS IN EXPOSURE TO VESICANTS AND THE
EFFICACY OF AN ANTIDOTE FORMULA IN DIFFERENT PHARMACEUTICAL FORMS. AN EXPERIMENTAL
APPROACH
85133314118&doi=10.31925%2ffarmacia.2022.3.11&partnerID=40&md5=10c16e6b15c788507ad3
2ec34d08e980
VL - 70
ID - 5141
ER -
TY - JOUR
AB - Background: The increasing use of silver nanoparticles (AgNPs) in consumer
products is concerning. We examined the potential toxic effects when inhaled in
Brown-Norway (BN) rats with a pre-inflammatory state compared to Sprague-Dawley
(SD) rats. Methods: We determined the effect of AgNPs generated from a spark
generator (mass concentration: 600-800 mu g/mm(3); mean diameter: 13-16 nm; total
lung doses: 8 [Low] and 26-28 [High] mu g) inhaled by the nasal route in both rat
strains. Rats were sacrificed at day 1 and day 7 after exposure and measurement of
lung function. Results: In both strains, there was an increase in neutrophils in
bronchoalveolar lavage (BAL) fluid at 24 h at the high dose, with concomitant
eosinophilia in BN rats. While BAL inflammatory cells were mostly normalised by Day
7, lung inflammation scores remained increased although not the tissue eosinophil
scores. Total protein levels were elevated at both lung doses in both strains.
There was an increase in BAL IL-1 beta, KC, IL-17, CCL2 and CCL3 levels in both
strains at Day 1, mostly at high dose. Phospholipid levels were increased at the
high dose in SD rats at Day 1 and 7, while in BN rats, this was only seen at Day 1;
surfactant protein D levels decreased at day 7 at the high dose in SD rats, but was
increased at Day 1 at the low dose in BN rats. There was a transient increase in
central airway resistance and in tissue elastance in BN rats at Day 1 but not in SD
rats. Positive silver-staining was seen particularly in lung tissue macrophages in
a dose and time-dependent response in both strains, maximal by day 7. Lung silver
levels were relatively higher in BN rat and present at day 7 in both strains.
Conclusions: Presence of cellular inflammation and increasing silver-positive
macrophages in lungs at day 7, associated with significant levels of lung silver
indicate that lung toxicity is persistent even with the absence of airway luminal
inflammation at that time-point. The higher levels and persistence of lung silver
in BN rats may be due to the pre-existing inflammatory state of the lungs.
AN - WOS:000379970200002
AU - Seiffert, J.
AU - Buckley, A.
AU - Leo, B.
AU - Martin, N. G.
AU - Zhu, J.
AU - Dai, R. R.
AU - Hussain, F.
AU - Guo, C.
AU - Warren, J.
AU - Hodgson, A.
AU - Gong, J. C.
AU - Ryan, M. P.
AU - Zhang, J. F.
AU - Porter, A.
AU - Tetley, T. D.
AU - Gow, A.
AU - Smith, R.
AU - Chung, K. F.
C7 - 85
DA - JUL 19
DO - 10.1186/s12931-016-0407-7
PY - 2016
SN - 1465-993X
1465-9921
ST - Pulmonary effects of inhalation of spark-generated silver nanoparticles in
Brown-Norway and Sprague-Dawley rats
T2 - RESPIRATORY RESEARCH
TI - Pulmonary effects of inhalation of spark-generated silver nanoparticles in
VL - 17
ID - 6087
ER -
TY - JOUR
AB - Background: The increasing use of silver nanoparticles (AgNPs) in consumer
products is concerning. We examined the potential toxic effects when inhaled in
Brown-Norway (BN) rats with a pre-inflammatory state compared to Sprague-Dawley
(SD) rats. Methods: We determined the effect of AgNPs generated from a spark
generator (mass concentration: 600-800 μg/mm3; mean diameter: 13-16 nm; total lung
doses: 8 [Low] and 26-28 [High] μg) inhaled by the nasal route in both rat strains.
Rats were sacrificed at day 1 and day 7 after exposure and measurement of lung
function. Results: In both strains, there was an increase in neutrophils in
bronchoalveolar lavage (BAL) fluid at 24 h at the high dose, with concomitant
eosinophilia in BN rats. While BAL inflammatory cells were mostly normalised by Day
7, lung inflammation scores remained increased although not the tissue eosinophil
scores. Total protein levels were elevated at both lung doses in both strains.
There was an increase in BAL IL-1β, KC, IL-17, CCL2 and CCL3 levels in both strains
at Day 1, mostly at high dose. Phospholipid levels were increased at the high dose
in SD rats at Day 1 and 7, while in BN rats, this was only seen at Day 1;
surfactant protein D levels decreased at day 7 at the high dose in SD rats, but was
increased at Day 1 at the low dose in BN rats. There was a transient increase in
central airway resistance and in tissue elastance in BN rats at Day 1 but not in SD
rats. Positive silver-staining was seen particularly in lung tissue macrophages in
a dose and time-dependent response in both strains, maximal by day 7. Lung silver
levels were relatively higher in BN rat and present at day 7 in both strains.
Conclusions: Presence of cellular inflammation and increasing silver-positive
macrophages in lungs at day 7, associated with significant levels of lung silver
indicate that lung toxicity is persistent even with the absence of airway luminal
inflammation at that time-point. The higher levels and persistence of lung silver
in BN rats may be due to the pre-existing inflammatory state of the lungs. © 2016
The Author(s).
AU - Seiffert, J.
AU - Buckley, A.
AU - Leo, B.
AU - Martin, N. G.
AU - Zhu, J.
AU - Dai, R.
AU - Hussain, F.
AU - Guo, C.
AU - Warren, J.
AU - Hodgson, A.
AU - Gong, J.
AU - Ryan, M. P.
AU - Zhang, J. J.
AU - Porter, A.
AU - Tetley, T. D.
AU - Gow, A.
AU - Smith, R.
AU - Chung, K. F.
C7 - 85
DB - Scopus
DO - 10.1186/s12931-016-0407-7
IS - 1
KW - Inflammation
Inhalation
Lungs
Silver nanospheres
Animals
Cytokines
Inhalation Exposure
Lung
Male
Metal Nanoparticles
Phospholipids
Pneumonia
Pulmonary Eosinophilia
Pulmonary Surfactant-Associated Protein D
Rats, Inbred BN
Respiratory Mechanics
Silver
Time Factors
interleukin 17
interleukin 1beta
keratinocyte derived chemokine
malonaldehyde
phospholipid
silver nanoparticle
surfactant protein D
autacoid
cytokine
metal nanoparticle
silver
airway resistance
animal experiment
animal tissue
Article
brown Norway rat
controlled study
eosinophilia
inhalation
lung compliance
lung toxicity
male
neutrophil
nonhuman
rat
respiratory tract inflammation
Sprague Dawley rat
animal
breathing mechanics
chemically induced
dose response
drug effects
exposure
immunology
Loeffler pneumonia
lung
metabolism
pathophysiology
pneumonia
time factor
M3 - Article
PY - 2016
ST - Pulmonary effects of inhalation of spark-generated silver nanoparticles in
T2 - Respiratory Research
TI - Pulmonary effects of inhalation of spark-generated silver nanoparticles in
84978863552&doi=10.1186%2fs12931-016-0407-
7&partnerID=40&md5=5e526cd5362903d07d96f87ecafdc7f1
VL - 17
ID - 5461
ER -
TY - JOUR
AB - Particle size and surface chemistry are potential determinants of silver
nanoparticle (AgNP) respiratory toxicity that may also depend on the lung
inflammatory state. We compared the effects of intratracheally-administered AgNPs
(20nm and 110nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in
Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a
neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic
response at day 1, greatest for the 20nm citrate-capped AgNPs. Eosinophilic
cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on
day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7
days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of
CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at
day 1, with persistence at day 7. The 20nm, but not the 110 nm, AgNPs increased
bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-
capped AgNPs only. The 20nm versus the 110 nm size were more proinflammatory in
terms of neutrophil influx, but there was little difference between the citrate-
capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and
neutrophilic inflammation with bronchial hyperresponsiveness, features
characteristic of asthma.
AN - WOS:000350689400086
AU - Seiffert, J.
AU - Hussain, F.
AU - Wiegman, C.
AU - Li, F.
AU - Bey, L.
AU - Baker, W.
AU - Porter, A.
AU - Ryan, M. P.
AU - Chang, Y.
AU - Gow, A.
AU - Zhang, J. F.
AU - Zhu, J.
AU - Tetley, T. D.
AU - Chung, K. F.
C7 - e0119726
DA - MAR 6
DO - 10.1371/journal.pone.0119726
IS - 3
PY - 2015
SN - 1932-6203
ST - Pulmonary Toxicity of Instilled Silver Nanoparticles: Influence of Size,
Coating and Rat Strain
T2 - PLOS ONE
TI - Pulmonary Toxicity of Instilled Silver Nanoparticles: Influence of Size,
Coating and Rat Strain
VL - 10
ID - 6146
ER -
TY - JOUR
AB - Particle size and surface chemistry are potential determinants of silver
nanoparticle (AgNP) respiratory toxicity that may also depend on the lung
inflammatory state. We compared the effects of intratracheally-administered AgNPs
(20nm and 110nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in
Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a
neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic
response at day 1, greatest for the 20nm citrate-capped AgNPs. Eosinophilic
cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on
day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7
days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of
CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at
day 1, with persistence at day 7. The 20nm, but not the 110 nm, AgNPs increased
bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-
capped AgNPs only. The 20nm versus the 110 nm size were more proinflammatory in
terms of neutrophil influx, but there was little difference between the citrate-
capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and
neutrophilic inflammation with bronchial hyperresponsiveness, features
characteristic of asthma. © 2015 Seiffert et al.
AU - Seiffert, J.
AU - Hussain, F.
AU - Wiegman, C.
AU - Li, F.
AU - Bey, L.
AU - Baker, W.
AU - Porter, A.
AU - Ryan, M. P.
AU - Chang, Y.
AU - Gow, A.
AU - Zhang, J.
AU - Zhu, J.
AU - Tetley, T. D.
AU - Chung, K. F.
C7 - e0119726
DB - Scopus
DO - 10.1371/journal.pone.0119726
IS - 3
KW - Animals
Citric Acid
Lung
Male
Metal Nanoparticles
Neutrophils
Particle Size
Povidone
Rats
Silver
Rattus
bronchoalveolar lavage protein
cell protein
citric acid
cytokine
eosinophil cationic protein
eotaxin
interferon
interleukin 13
keratinocyte chemoattractant
malonaldehyde
povidone
silver nanoparticle
unclassified drug
metal nanoparticle
polyvinylpolypyrrolidone
silver
allergic asthma
animal cell
animal experiment
animal model
animal tissue
Article
brown Norway rat
CCL11 gene
cell transport
controlled study
drug instillation
dynamics
eosinophilia
gene expression
hydrodynamics
IFN gene
IL 5 gene
light scattering
Loeffler pneumonia
lung lavage
lung parenchyma
lung resistance
lung toxicity
material coating
neutrophil
neutrophilia
nonhuman
particle size
protein expression
rat
respiratory tract allergy
surface property
turbidity
zeta potential
analogs and derivatives
animal
drug effects
lung
male
metabolism
pathology
Sprague Dawley rat
M3 - Article
PY - 2015
ST - Pulmonary toxicity of instilled silver nanoparticles: Influence of size,
coating and rat strain
T2 - PLoS ONE
TI - Pulmonary toxicity of instilled silver nanoparticles: Influence of size,
coating and rat strain
84924205840&doi=10.1371%2fjournal.pone.0119726&partnerID=40&md5=2d9a2032cb06a81c069
bfca3cddb6433
VL - 10
ID - 5653
ER -
TY - JOUR
AB - The blood compatibility of AgNPs is of great relevance as it has good
antifungal, antibacterial and anti-inflammatory properties and the toxicological
information of their effects on cells need to be analyzed before using it as drug
carriers in the biomedical field. The present study deals with the synthesis of
AgNPs from an aqueous solution of silver nitrate using Acalypha hispida leaf
extract as the reducing and capping agent. The presence of AgNPs in the reaction
mixture was confirmed by visual observation of color change and subsequently
identified using UV-Visible Spectroscopy. XRD results revealed the crystalline
nature of synthesized AgNPs. The shape and size of particles were characterized by
TEM. These results revealed the elemental status of nanopowder. The components
present in leaf extract were identified by GC-MS and functional groups present in
the sample when treated with silver nitrate were obtained from FT-IR results. The
surface of synthesized AgNPs was modified using four different compounds such as
CTAB, PEG, PEI, and APTMS to evaluate the blood compatibility. The results showed
that 50 mu g/mL CTAB coated AgNPs and 50 and 100 mu g/mL PEG coated AgNPs had non-
hemolytic property and considered as more blood compatible surface modified AgNPs.
This investigation gives an idea of using surface modified AgNPs in the field of
biomedicine and therapeutic applications.
AN - WOS:000432501300007
AU - Selvakumar, P.
AU - Sithara, R.
AU - Viveka, K.
AU - Sivashanmugam, P.
DA - MAY
DO - 10.1016/j.jphotobiol.2018.03.018
PY - 2018
SN - 1011-1344
SP - 52-61
ST - Green synthesis of silver nanoparticles using leaf extract of Acalypha
hispida and its application in blood compatibility
T2 - JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
TI - Green synthesis of silver nanoparticles using leaf extract of Acalypha
hispida and its application in blood compatibility
VL - 182
ID - 6058
ER -
TY - JOUR
AB - Toxoplasma gondii, a protozoan parasite, is responsible for toxoplasmosis.
The available therapy for patients with toxoplasmosis involves a combination of
pyrimethamine and sulfadiazine, which have several adverse effects, including bone
marrow suppression, megaloblastic anemia, leukopenia, and granulocytopenia. The
development of therapeutic alternatives is essential for the management of
toxoplasmosis, emphasizing the recent advances in nanomedicine. This study aimed to
evaluate the in vitro effects of biogenic silver nanoparticles (AgNp-Bio) on
tachyzoite forms and Leydig cells infected with T. gondii. We observed that the
AgNp-Bio reduced the viability of the tachyzoites and did not exhibit cytotoxicity
against Leydig cells at low concentrations. Additionally, treatment with AgNp-Bio
reduced the rate of infection and proliferation of the parasite, and lowered the
testosterone levels in the infected cells. It increased the levels of IL-6 and TNF-
α and reduced the levels of IL- 10. Among the morphological and ultrastructural
changes, AgNp-Bio induced a reduction in the number of intracellular tachyzoites
and caused changes in the tachyzoites with accumulation of autophagic vacuoles and
a decrease in the number of tachyzoites inside the parasitophorous vacuoles.
Collectively, our data demonstrate that the AgNp-Bio affect T. gondii tachyzoites
by activating microbicidal and inflammatory mechanisms and could be a potential
alternative treatment for toxoplasmosis. © 2022 Elsevier Inc.
AU - Semedo, S. S. L.
AU - da Silva Sanfelice, R. A.
AU - Silva, T. F.
AU - da Silva Bortoleti, B. T.
AU - de Oliveira, G. C.
AU - de Lion Siervo, G. E. M.
AU - Bosqui, L. R.
AU - Lazarin-Bidói, D.
AU - Garcia, J. L.
AU - Nakazato, G.
AU - Fernandes, G. S. A.
AU - da Costa, I. N.
C7 - 108343
DB - Scopus
DO - 10.1016/j.exppara.2022.108343
KW - Biogenic silver nanoparticles (AgNp-Bio)
Cytotoxicity
Leydig (TM3) cells
Testosterone
Toxoplasmosis
Humans
Interleukin-6
Leydig Cells
Male
Metal Nanoparticles
Silver
Toxoplasma
interleukin 6
metal nanoparticle
silver
testosterone
human
Leydig cell
male
toxoplasmosis
M3 - Article
PY - 2022
ST - Biogenic silver nanoparticles (AgNp-Bio) restore testosterone levels and
increase TNF-α and IL-6 in Leydig cells infected with Toxoplasma gondii
T2 - Experimental Parasitology
TI - Biogenic silver nanoparticles (AgNp-Bio) restore testosterone levels and
increase TNF-α and IL-6 in Leydig cells infected with Toxoplasma gondii
85135901276&doi=10.1016%2fj.exppara.2022.108343&partnerID=40&md5=2348fcbbac8510a5fd
f6a87f663c150c
VL - 241
ID - 5134
ER -
TY - JOUR
AB - Toxoplasma gondii, a protozoan parasite, is responsible for toxoplasmosis.
The available therapy for patients with toxoplasmosis involves a combination of
pyrimethamine and sulfadiazine, which have several adverse effects, including bone
marrow suppression, megaloblastic anemia, leukopenia, and granulocytopenia. The
development of therapeutic alternatives is essential for the management of
toxoplasmosis, emphasizing the recent advances in nanomedicine. This study aimed to
evaluate the in vitro effects of biogenic silver nanoparticles (AgNp-Bio) on
tachyzoite forms and Leydig cells infected with T. gondii. We observed that the
AgNp-Bio reduced the viability of the tachyzoites and did not exhibit cytotoxicity
against Leydig cells at low concentrations. Additionally, treat-ment with AgNp-Bio
reduced the rate of infection and proliferation of the parasite, and lowered the
testosterone levels in the infected cells. It increased the levels of IL-6 and TNF-
alpha and reduced the levels of IL-10. Among the morphological and ultrastructural
changes, AgNp-Bio induced a reduction in the number of intracellular tachyzoites
and caused changes in the tachyzoites with accumulation of autophagic vacuoles and
a decrease in the number of tachyzoites inside the parasitophorous vacuoles.
Collectively, our data demonstrate that the AgNp-Bio affect T. gondii tachyzoites
by activating microbicidal and inflammatory mechanisms and could be a potential
alternative treatment for toxoplasmosis.
AN - WOS:000843008600002
AU - Semedo, S. S. L.
AU - Sanfelice, R. A. D.
AU - Silva, T. F.
AU - Bortoleti, B. T. D.
AU - de Oliveira, G. C.
AU - Siervo, Gemd
AU - Bosqui, L. R.
AU - Lazarin-Bidoi, D.
AU - Garcia, J. L.
AU - Nakazato, G.
AU - Fernandes, G. S. A.
AU - Costa, I. N. D.
C6 - AUG 2022
C7 - 108343
DA - OCT
DO - 10.1016/j.exppara.2022.108343
PY - 2022
SN - 0014-4894
1090-2449
ST - Biogenic silver nanoparticles (AgNp-Bio) restore testosterone levels and
increase TNF-alpha and IL-6 in Leydig cells infected with Toxoplasma gondii
T2 - EXPERIMENTAL PARASITOLOGY
TI - Biogenic silver nanoparticles (AgNp-Bio) restore testosterone levels and
increase TNF-alpha and IL-6 in Leydig cells infected with Toxoplasma gondii
VL - 241
ID - 5960
ER -
TY - CHAP
AB - Infective diseases have become health threat of a global proportion due to
appearance and spread of microorganisms resistant to majority of therapeutics
currently used for their treatment. Therefore, there is a constant need for
development of new antimicrobial agents, as well as novel therapeutic strategies.
Quinolines and quinolones, isolated from plants, animals, and microorganisms, have
demonstrated numerous biological activities such as antimicrobial, insecticidal,
anti-inflammatory, antiplatelet, and antitumor. For more than two centuries
quinoline/quinolone moiety has been used as a scaffold for drug development and
even today it represents an inexhaustible inspiration for design and development of
novel semi-synthetic or synthetic agents exhibiting broad spectrum of
bioactivities. The structural diversity of synthetized compounds provides high and
selective activity attained through different mechanisms of action, as well as low
toxicity on human cells. This review describes quinoline and quinolone derivatives
with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic
activities with the focus on the last 10 years literature. © Springer Nature
Switzerland AG 2019.
AU - Senerovic, L.
AU - Opsenica, D.
AU - Moric, I.
AU - Aleksic, I.
AU - Spasić, M.
AU - Vasiljevic, B.
DB - Scopus
DO - 10.1007/5584_2019_428
KW - Anti-parasitics
Anti-virulence activity
Antibiotics
Antifungals
Antivirals
Quinoline/quinolone derivatives
Animals
Antifungal Agents
Antiparasitic Agents
Antiviral Agents
Humans
Quinolines
Quinolones
Virulence
2 quinolone
4 quinolone
8 quinolinol derivative
amodiaquine
antibiotic agent
antifungal agent
antileishmanial agent
antimalarial agent
antiparasitic agent
antitrypanosomal agent
antivirus agent
bedaquiline
besifloxacin
broxyquinoline
chloroquine
ciprofloxacin
levofloxacin
mefloquine
molecular scaffold
moxifloxacin
nalidixic acid
norfloxacin
primaquine
quinine
quinoline
quinoline derivative
sulfadiazine silver
tefenoquine
unclassified drug
unindexed drug
antiinfective agent
animal
antiplatelet activity
drug design
drug development
drug isolation
drug potency
drug selectivity
drug structure
human
insecticidal activity
microorganism
nonhuman
plant
priority journal
drug effect
virulence
PY - 2020
SP - 37-69
ST - Quinolines and quinolones as antibacterial, antifungal, anti-virulence,
antiviral and anti-parasitic agents
T2 - Advances in Experimental Medicine and Biology
TI - Quinolines and quinolones as antibacterial, antifungal, anti-virulence,
antiviral and anti-parasitic agents
85089126743&doi=10.1007%2f5584_2019_428&partnerID=40&md5=c229e6d5bea3b3ef936f5533e0
5f1261
VL - 1282
ID - 5377
ER -
TY - JOUR
AB - Necroptosis is a regulated cell death that is governed by mixed lineage
kinase domain-like, receptor-interacting serine-threonine kinase 3 and commonly
displays with necrosis morphological characteristics. This study examined the
molecular mechanisms involved in the chemical-induced necroptosis where a
systematic evaluation of experimental studies addressing this issue is missing. We
strictly reviewed all scientific reports related to our search terms including
“necroptosis” or “programmed necrosis”, “environmental chemicals” or “air
pollutants” or “pesticides” or “nanoparticles” and “Medicines” from 2009 to 2019.
Manuscripts that met the objective of this study were included for further
evaluations. Studies showed that several pathological contexts like cancer,
neurodegenerative disorders, and inflammatory diseases were related to necroptosis.
Furthermore, multiple chemical-induced cytotoxic effects, such as DNA damage,
mitochondrial dysregulation, oxidative damage, lipid peroxidation, endoplasmic
reticulum disruption, and inflammation are also associated with necroptosis. The
main environmental exposures that are related to necroptosis are air pollutants
(airborne particulate matter, cadmium, and hydrogen sulfide), nanoparticles (gold,
silver, and silica), pesticides (endosulfan, cypermethrin, chlorpyrifos, and
paraquat), and tobacco smoke. To sum up, air pollutants, pesticides, and
nanoparticles could potentially affect human health via disruption of cell growth
and induction of necroptosis. Understanding the exact molecular pathogenesis of
these environmental chemicals needs further comprehensive research to provide
innovative concepts for the prevention approaches and introduce novel targets for
the amelioration of a range of human health problems. © 2020, Springer-Verlag GmbH
Germany, part of Springer Nature.
AU - Sepand, M. R.
AU - Aliomrani, M.
AU - Hasani-Nourian, Y.
AU - Khalhori, M. R.
AU - Farzaei, M. H.
AU - Sanadgol, N.
DB - Scopus
DO - 10.1007/s11356-020-09360-5
IS - 30
KW - Apoptosis
Cell death
Environmental chemicals
Nanoparticles
Necroptosis
Humans
Inflammation
Necrosis
Receptor-Interacting Protein Serine-Threonine Kinases
Nicotiana tabacum
receptor interacting protein serine threonine kinase
cancer
cell component
cell organelle
DNA
enzyme
enzyme activity
experimental study
growth
induced response
morphology
nervous system disorder
apoptosis
human
inflammation
necroptosis
necrosis
M3 - Review
PY - 2020
SP - 37488-37501
ST - Mechanisms and pathogenesis underlying environmental chemical-induced
necroptosis
T2 - Environmental Science and Pollution Research
TI - Mechanisms and pathogenesis underlying environmental chemical-induced
necroptosis
85088098972&doi=10.1007%2fs11356-020-09360-
5&partnerID=40&md5=9e0883072430bb1a56184b58f71797be
VL - 27
ID - 5337
ER -
TY - JOUR
AB - The combination of silver (Ag) and titanium dioxide (TiO2) nanostructures
offer unique advantages in terms of elimination of infection and enhanced
antibacterial activity with relatively higher biocompatibility and lower
cytotoxicity. Although there have been numerous attempts for the fabrication of
these nanocomposite systems, novel, flexible, low-cost, simple, effective,
reducing, and stabilizing agent-free strategies are highly required for biomedical
applications. Within this context, we report the employment of silver nanostructure
decorated TiO2 nanowires (TiO2 NWs) as an ideal antibacterial agent against
antibiotic-resistant Gram-negative (Escherichia coli) and Gram-positive bacteria
(Staphylococcus aureus). Firstly, TiO2 NWs were fabricated via the hydrothermal
procedure. Afterward, by utilizing the oxidative polymerization of 3,4-
dihydroxyphenyl-L-alanine (L-DOPA), a conformal and thin polymer layer of L-DOPA
(PLDP) was created onto the TiO2 NWs (TiO2/PLDP). Lastly, Ag nanostructures were
deposited onto the TiO2/PLDP (TiO2/PLDP/Ag NP) via simply reduction of silver ions.
Herein, PLDP with its abundant catechol and amine groups played an important role
in the reduction of silver ions and the adsorption of Ag nanostructures with high
affinity and resultant stability. The size, density, and morphology of Ag
nanostructures were manipulated by tuning the initial amount of Ag ions in a well-
controlled manner. The resultant colloidal TiO2/PLDP/Ag composite nanosystem
provided remarkably high and stable antibacterial activity against both antibiotic-
resistant bacteria strains. Minimum Inhibitory Concentration (MIC) values were
found to be 125, 250, and > 500 ppm for high, medium and, low deposition of Ag
nanostructures, respectively. Similarly, Minimum Bactericidal Concentration (MBC)
for these NP systems, MBC values were found to be 250, 500, and > 1000 ppm,
respectively. Also, relatively lower cytotoxicity in human lung healthy (MRC5) and
cancer (A549) cell lines was detected in a dose-dependent manner in comparison to
the citrate-stabilized Ag nanoparticles. The proposed novel TiO2/PLDP/Ag nanosystem
will provide unique opportunities in terms of flexibility, low-cost, simplicity
with reducing, and stabilizing agent-free strategy and be employed in the removal
of biofilms and anti-inflammatory effects.
AN - WOS:000810781300002
AU - Serginay, N.
AU - Dizaji, A. N.
AU - Mazlumoglu, H.
AU - Karatas, E.
AU - Yilmaz, A.
AU - Yilmaz, M.
C6 - JAN 2022
C7 - 128350
DA - APR 20
DO - 10.1016/j.colsurfa.2022.128350
PY - 2022
SN - 0927-7757
1873-4359
ST - Antibacterial activity and cytotoxicity of bioinspired poly (L-DOPA)-mediated
silver nanostructure-decorated titanium dioxide nanowires
T2 - COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
TI - Antibacterial activity and cytotoxicity of bioinspired poly (L-DOPA)-mediated
VL - 639
ID - 6097
ER -
TY - JOUR
AB - The combination of silver (Ag) and titanium dioxide (TiO2) nanostructures
offer unique advantages in terms of elimination of infection and enhanced
antibacterial activity with relatively higher biocompatibility and lower
cytotoxicity. Although there have been numerous attempts for the fabrication of
these nanocomposite systems, novel, flexible, low-cost, simple, effective,
reducing, and stabilizing agent-free strategies are highly required for biomedical
applications. Within this context, we report the employment of silver nanostructure
decorated TiO2 nanowires (TiO2 NWs) as an ideal antibacterial agent against
antibiotic-resistant Gram-negative (Escherichia coli) and Gram-positive bacteria
(Staphylococcus aureus). Firstly, TiO2 NWs were fabricated via the hydrothermal
procedure. Afterward, by utilizing the oxidative polymerization of 3,4-
dihydroxyphenyl-L-alanine (L-DOPA), a conformal and thin polymer layer of L-DOPA
(PLDP) was created onto the TiO2 NWs (TiO2/PLDP). Lastly, Ag nanostructures were
deposited onto the TiO2/PLDP (TiO2/PLDP/Ag NP) via simply reduction of silver ions.
Herein, PLDP with its abundant catechol and amine groups played an important role
in the reduction of silver ions and the adsorption of Ag nanostructures with high
affinity and resultant stability. The size, density, and morphology of Ag
nanostructures were manipulated by tuning the initial amount of Ag ions in a well-
controlled manner. The resultant colloidal TiO2/PLDP/Ag composite nanosystem
provided remarkably high and stable antibacterial activity against both antibiotic-
resistant bacteria strains. Minimum Inhibitory Concentration (MIC) values were
found to be 125, 250, and > 500 ppm for high, medium and, low deposition of Ag
nanostructures, respectively. Similarly, Minimum Bactericidal Concentration (MBC)
for these NP systems, MBC values were found to be 250, 500, and > 1000 ppm,
respectively. Also, relatively lower cytotoxicity in human lung healthy (MRC5) and
cancer (A549) cell lines was detected in a dose-dependent manner in comparison to
the citrate-stabilized Ag nanoparticles. The proposed novel TiO2/PLDP/Ag nanosystem
will provide unique opportunities in terms of flexibility, low-cost, simplicity
with reducing, and stabilizing agent-free strategy and be employed in the removal
of biofilms and anti-inflammatory effects. © 2022 Elsevier B.V.
AU - Serginay, N.
AU - Dizaji, A. N.
AU - Mazlumoglu, H.
AU - Karatas, E.
AU - Yilmaz, A.
AU - Yilmaz, M.
C7 - 128350
DB - Scopus
DO - 10.1016/j.colsurfa.2022.128350
Cytotoxicity
Poly(L-DOPA)
Silver nanostructures
Titanium dioxide nanowires
Antibiotics
Biocompatibility
Cell culture
Cost reduction
Escherichia coli
Metal ions
Morphology
Nanocomposites
Nanowires
Silver compounds
TiO2 nanoparticles
levodopa
nanocomposite
nanoparticle
nanowire
protein bcl 2
silver
silver nanoparticle
titanium dioxide
% reductions
Low cytotoxicities
Low-costs
Polymer layers
Stabilizing agents
TiO 2
A-549 cell line
adsorption
apoptosis
Article
bacterial clearance
bacterial growth
bacterial strain
biocompatibility
biofilm
broth dilution
Candida albicans
cell viability
controlled study
cytotoxicity
dispersity
enzyme activity
human
human cell
hydrogen peroxide scavenging assay
LD50
MRC-5 cell line
MTT assay
thermostability
Titanium dioxide
M3 - Article
PY - 2022
ST - Antibacterial activity and cytotoxicity of bioinspired poly(L-DOPA)-mediated
TI - Antibacterial activity and cytotoxicity of bioinspired poly(L-DOPA)-mediated
85122925353&doi=10.1016%2fj.colsurfa.2022.128350&partnerID=40&md5=9fdc13227fe97a574
3758387a54f31e8
VL - 639
ID - 5085
ER -
TY - JOUR
AB - Wound tissue regeneration and angiogenesis are dynamic processes that send
physiological signals to the body. Thus, designing novel nanoscaffolds by
understanding their surface modifications and toxicological response in a
biological system with a potent anti-inflammatory response is a viable solution. In
this respect, inspired by the surface chemistry, in the present work we focus on
the chemical optimization of silver nanoscaffolds using surface cappings in order
to understand their kinetic release behaviour in simulated wound fluids (SWF), to
analyze their blood compatibility in human lymphocytes and erythrocytes and then
embed them in a chitosan-agarose matrix (CAM) as a productive drug delivery system
to evaluate in vivo excision wound tissue regeneration efficiency in Wistar rats.
In this regard, polyvinyl alcohol capped silver nanocomposites (PVA-AgNPs) exhibit
a dominant antibacterial efficacy with the sustained and controlled release of
silver ions and percentage cell mortality and percentage hemolysis of only 10% and
16% compared with uncapped-AgNPs or silver bandaids (SBDs). Also, PVA-AgNP
impregnated CAM (PVA-CAM) shows positive effects through their anti-inflammatory
and angiogenic properties, with a nearly 95% healing effect within 9 days. The
complete development of collagen and fibroblast constituents was also monitored in
PVA-CAM by hematoxylin & eosin (H & E) and Masson trichrome (MT) staining. These
results provide a clear insight into the development of a potent therapeutic
formulation using CAM as a scaffold incorporated with surface functionalized PVA-
AgNPs as a bioeffective and biocompatible polymer for the fabrication of
efficacious silver wound dressing scaffolds in clinical practice. © 2019 The Royal
Society of Chemistry.
AU - Sethuram, L.
AU - Thomas, J.
AU - Mukherjee, A.
AU - Chandrasekaran, N.
DB - Scopus
DO - 10.1039/c9ra06913e
IS - 61
Blood
Cams
Cell culture
Chemical analysis
Functional polymers
Metal ions
Rats
Scaffolds (biology)
Silver
Surface chemistry
Tissue
Anti-inflammatory response
Antibacterial efficacy
Biocompatible polymer
Chemical optimizations
Physiological signals
Regeneration efficiency
Simulated wound fluids
Toxicological response
Tissue regeneration
M3 - Article
PY - 2019
SP - 35677-35694
ST - Effects and formulation of silver nanoscaffolds on cytotoxicity dependent ion
release kinetics towards enhanced excision wound healing patterns in Wistar albino
rats
T2 - RSC Advances
TI - Effects and formulation of silver nanoscaffolds on cytotoxicity dependent ion
release kinetics towards enhanced excision wound healing patterns in Wistar albino
rats
85074828410&doi=10.1039%2fc9ra06913e&partnerID=40&md5=34ee99e142c9af32a013b28557abd
11b
VL - 9
ID - 5418
ER -
TY - JOUR
AB - Background and purpose: Despite the widespread use of silver nanoparticles,
there are concerns about their biological effects on the environment and human
health. The aim of this study was to investigate the cytotoxic effect of Ag+
nanoparticles on liver tissue and enzyme activities in NMRI mice. Materials and
methods: In this experimental study, thirty five female NMRI mice were randomly
divided into one control group and four experimental groups (n=7 per group). The
mice in experimental groups were injected with silver nanoparticles at different
concentrations (50, 100, 200, and 400 mg/kg intraperitoneally, every other day).
Blood samples were taken from the inner corners of the eyes of mice and after
separating the serum, liver enzyme activities were analyzed. Then, all animals were
euthanized via cervical dislocation and tissue samples were stained with
hematoxylin and eosin for histopathology evaluation. Statistical analysis was
performed using SAS software. Results: Histopathological examination showed that
different concentrations of Ag+ nanoparticles resulted in mild to severe injury
(necrosis, inflammatory cell infiltration, and vacuolar degeneration) in the liver.
The activity of liver enzymes significantly increased in all groups at 400 mg/kg
concentration compared to that of the control group. Conclusion: Our findings
indicated that exposure to different concentrations of Ag+ nanoparticles can cause
severe damages in the mice liver and increase serum enzyme activities. © 2015,
Mazandaran University of Medical Sciences. All rights reserved.
AU - Seyedalipour, B.
AU - Arefifar, A.
AU - Khanbabaee, R.
AU - Hoseini, S. M.
DB - Scopus
IS - 124
KW - Inflammatory cell infiltration
Necrosis
NMRI mice
Transaminase
silver nanoparticle
animal experiment
animal model
animal tissue
Article
cell infiltration
controlled study
cytotoxicity
enzyme activity
female
histopathology
liver toxicity
mouse
necrosis
NMRI mouse
nonhuman
M3 - Article
PY - 2015
SP - 183-193
ST - Toxicity of silver nanoparticles on ALT, AST, ALP and histopathological
changes in NMRI mice
T2 - Journal of Mazandaran University of Medical Sciences
TI - Toxicity of silver nanoparticles on ALT, AST, ALP and histopathological
changes in NMRI mice
85008397796&partnerID=40&md5=81794fd191d1d7655da545cf00e6f850
VL - 25
ID - 5609
ER -
TY - JOUR
AB - New facile way for production of antimicrobial wound healing dress was
achieved using freshly fabricated bacterial cellulose (BNC) decorated with in situ
formed silver nanoparticles (AgNPs). Herein, BNC was fabricated by using a domestic
isolated strain Komagataeibacter hansenii SA1.1. BNC/AgNPs nanocomposites were in
situ synthesized by introducing AgNO3 to alkaline BNC at 60 °C for 30 min to attain
full chemical reduction of Ag ions which manifested visually by color change.
Results signified the successful fabrication of nanofibrillated BC with network
structure. Also, AgNPs were successfully assembled on the surface of BNC in
spherical shape with distribution uniformity consists of narrow size particles lied
in range of 10–40 nm. The hybrid BNC/AgNPs manifested a strong antibacterial
activity against Gram negative and Gram positive as well as unicellular fungus. The
results from in vitro study demonstrated, undoubtedly, that BNC/AgNPs can be able
to promote the wound healing affinity of chronic ulcers through reducing microbial
infection besides accelerating the healing by their inflammatory. While, the
antioxidant affirmed the potential protection of BNC/AgNPs from oxidative
stressors, it was displayed a vital role in the improvement cell propagation and
cell proliferation. © 2021, The Author(s), under exclusive licence to Springer
Science+Business Media, LLC, part of Springer Nature.
AU - Shaheen, T. I.
AU - El-Gamal, M. S.
AU - Desouky, S. E.
AU - Hassan, S. E. D.
AU - Alemam, A. M.
DB - Scopus
DO - 10.1007/s10876-021-02190-6
IS - 6
KW - Antimicrobial
Antioxidant
Bacterial cellulose
Wound healing
M3 - Article
PY - 2022
SP - 2735-2751
ST - Benign Production of AgNPs/Bacterial Nanocellulose for Wound Healing Dress:
Antioxidant, Cytotoxicity and In Vitro Studies
TI - Benign Production of AgNPs/Bacterial Nanocellulose for Wound Healing Dress:
85117726588&doi=10.1007%2fs10876-021-02190-
6&partnerID=40&md5=95382909a87d6c3d46dbe2a056a01bab
VL - 33
ID - 5127
ER -
TY - JOUR
AB - New facile way for production of antimicrobial wound healing dress was
achieved using freshly fabricated bacterial cellulose (BNC) decorated with in situ
formed silver nanoparticles (AgNPs). Herein, BNC was fabricated by using a domestic
isolated strain Komagataeibacter hansenii SA1.1. BNC/AgNPs nanocomposites were in
situ synthesized by introducing AgNO3 to alkaline BNC at 60 degrees C for 30 min to
attain full chemical reduction of Ag ions which manifested visually by color
change. Results signified the successful fabrication of nanofibrillated BC with
network structure. Also, AgNPs were successfully assembled on the surface of BNC in
spherical shape with distribution uniformity consists of narrow size particles lied
in range of 10-40 nm. The hybrid BNC/AgNPs manifested a strong antibacterial
activity against Gram negative and Gram positive as well as unicellular fungus. The
results from in vitro study demonstrated, undoubtedly, that BNC/AgNPs can be able
to promote the wound healing affinity of chronic ulcers through reducing microbial
infection besides accelerating the healing by their inflammatory. While, the
antioxidant affirmed the potential protection of BNC/AgNPs from oxidative
stressors, it was displayed a vital role in the improvement cell propagation and
cell proliferation.
AN - WOS:000710056000001
AU - Shaheen, T. I.
AU - El-Gamal, M. S.
AU - Desouky, S. E.
AU - Hassan, S. E.
AU - Alemam, A. M.
C6 - OCT 2021
DA - NOV
DO - 10.1007/s10876-021-02190-6
IS - 6
PY - 2022
SN - 1040-7278
1572-8862
SP - 2735-2751
ST - Benign Production of AgNPs/Bacterial Nanocellulose for Wound Healing Dress:
TI - Benign Production of AgNPs/Bacterial Nanocellulose for Wound Healing Dress:
VL - 33
ID - 6172
ER -
TY - JOUR
AB - Recently, we have published a pioneering work on green biosynthesis and
complete characterization of gold and core shell silver-gold nanoparticles (AuNPs
and Ag@AuNPs). Herein, the so obtained nanoparticles are assessed for their
antidiabetic activity in streptozotocin-induced diabetic rats. Thus, sixty-four
male albino rats were divided into eight groups: control untreated; diabetic rats;
diabetic rats received standard drug; diabetic rats received carrier only; diabetic
rats received 0.5 ml AuNPs; diabetic rats received 1 ml AuNPs; diabetic rats
received 0.5 ml Ag@AuNPs and diabetic rats received 1 ml Ag@AuNPs for twenty-one
days. Results revealed that diabetic rats treated with AuNPs or Ag@AuNPs restored
normal glucose level. In particular, Ag@AuNPs was found to significantly induce a
reduction in blood glucose and restore both the high serum insulin level and
glucokinase activity compared to the control normal rats. The results obtained
disclose the effectual role of Ag@AuNPs in reducing the lipid profile, an anti-
inflammatory effect in diabetic rats assessed using inflammatory markers IL-α and
C-reactive protein (CRP). Histopathological examination of diabetic rats signifies
distortion in the arrangement of cells around the central vein, inflammatory cells,
pyknotic and apoptotic nuclei. Kidney of diabetic rat appears with vacuolation and
pyknotic nuclei of some tubules. On the other hand, the liver of diabetic rat
treated with Ag@AuNPs displayed normal hepatic cells with only few necrosis of
hepatocytes. Ag@AuNPs restored the increased number of caspase-3 stained cells in
the liver and kidney tissue in diabetic rats. In conclusion, Ag@AuNPs was observed
to improve diabetic condition by limiting prolonged inflammation, suppressing
oxidative stress and elevating the antioxidant defense system in diabetic rats
which subsequently evoke the potential impact of AuNPs as a cost effective
therapeutic cure in diabetic treatments and its complications. © 2016 Elsevier
Masson SAS
AU - Shaheen, T. I.
AU - El-Naggar, M. E.
AU - Hussein, J. S.
AU - El-Bana, M.
AU - Emara, E.
AU - El-Khayat, Z.
AU - Fouda, M. M. G.
AU - Ebaid, H.
AU - Hebeish, A.
DB - Scopus
DO - 10.1016/j.biopha.2016.07.052
KW - Blood glucose
Core shell silver–gold nanoparticles
Diabetic
Gold nanoparticles
Animals
Antioxidants
Blood Glucose
Caspase 3
Fasting
Gold
Hypoglycemic Agents
Inflammation
Insulin
Kidney
Lipids
Liver
Male
Metal Nanoparticles
Nitric Oxide
Oxidation-Reduction
Rats
Silver
Streptozocin
antidiabetic agent
antilipemic agent
antioxidant
C reactive protein
caspase 3
glucokinase
glucose
gold nanoparticle
insulin
silver nanoparticle
triacylglycerol
glucose blood level
gold
lipid
metal nanoparticle
nitric oxide
silver
streptozocin
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
cardiovascular risk
controlled study
drug potency
enzyme inhibition
green chemistry
immunoreactivity
in vivo study
insulin blood level
liver necrosis
male
nanomedicine
nonhuman
oxidative stress
particle size
priority journal
rat
triacylglycerol blood level
animal
blood
chemistry
diet restriction
drug effects
inflammation
kidney
liver
metabolism
pathology
ultrastructure
M3 - Article
PY - 2016
SP - 865-875
ST - Antidiabetic assessment; in vivo study of gold and core-shell silver-gold
nanoparticles on streptozotocin-induced diabetic rats
T2 - Biomedicine and Pharmacotherapy
TI - Antidiabetic assessment; in vivo study of gold and core-shell silver-gold
nanoparticles on streptozotocin-induced diabetic rats
84982275673&doi=10.1016%2fj.biopha.2016.07.052&partnerID=40&md5=3633d744d17ba0f8253
1b41da4e7a50a
VL - 83
ID - 5553
ER -
TY - JOUR
AB - Tissue engineering techniques are continuously evolving towards providing
better microenvironment along with therapeutic potential to address the skin tissue
defects. Factors such as microbial infections, presence of excessive free radicals
and depletion in antioxidant based scavenging systems pose serious challenges by
prolonging inflammation and delaying the repair process. Incorporation of bioactive
molecules in polymer based biomimetic scaffolds may present new vistas for handling
chronic wounds. In this study, chitosan/collagen scaffolds incorporating 0.5, 1 and
2% (w/w) silymarin (CS-CO-SM) were synthesized and studied for their
biocompatibility, in vitro release kinetics and anti-oxidant activity. The release
kinetics of silymarin from the CS-CO-SM scaffold showed an initial burst followed
by sustained release. The scaffolds were biocompatible and supported the recovery
of COS-7 cells from UV induced oxidative stress. Further the CS-CO-SM (2) scaffolds
were used to fabricate a bi-layer scaffold by layer upon layer arrangement with CS-
Ag3 (3% Ag, w/w). The Ag was incorporated to impart antimicrobial property to the
scaffold. The in vivo studies on bi-layer scaffolds were carried out in Wistar rat
models at 3, 7 and 10 days post injury and the skin excisions were studied for
wound contraction, histology (H&E staining), and lipid peroxidation. The bi-layer
scaffold accelerated the process of wound healing with no inflammatory cells,
proliferation of fibroblast, neovascularization and collagen deposition. By day 10
post transplantation of the scaffold, the skin had a structure similar to normal
skin with complete re-epithelization. This bi-layer scaffold with antioxidant and
antimicrobial properties promotes wound healing and is proposed as a potential
tissue engineering material for managing chronic wounds. [Figure not available: see
fulltext.]. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
AU - Shaik, M. M.
AU - Dapkekar, A.
AU - Rajwade, J. M.
AU - Jadhav, S. H.
AU - Kowshik, M.
C7 - 13
DB - Scopus
DO - 10.1007/s10856-018-6212-8
IS - 1
KW - Animals
Antioxidants
Cercopithecus aethiops
COS Cells
Lipid Peroxidation
Oxidative Stress
Random Allocation
Rats
Rats, Wistar
Tissue Engineering
Tissue Scaffolds
Wound Healing
antiinfective agent
antioxidant
chitosan
collagen
silver
silymarin
animal experiment
animal model
animal tissue
Article
biocompatibility
controlled study
excision
in vitro study
in vivo study
infection
nonhuman
oxidative stress
priority journal
rat
wound
wound contraction
wound healing
animal
bacterial infection
Chlorocebus aethiops
CV-1 cell line
drug effect
lipid peroxidation
procedures
randomization
tissue engineering
tissue scaffold
Wistar rat
M3 - Article
PY - 2019
ST - Antioxidant-antibacterial containing bi-layer scaffolds as potential
candidates for management of oxidative stress and infections in wound healing
TI - Antioxidant-antibacterial containing bi-layer scaffolds as potential
candidates for management of oxidative stress and infections in wound healing
85059896119&doi=10.1007%2fs10856-018-6212-
8&partnerID=40&md5=dddcdd66f4373d7e688c7557c233fd0a
VL - 30
ID - 5431
ER -
TY - JOUR
AB - Objective: Delonix regia (L.) (Caesalpiniaceae) is one of the plants that
have been used for centuries to prevent cancer. D. regia has been described as a
strong anti-inflammatory, antibacterial, and analgesic. Also, the cytotoxicity of
this plant has been proved due to its flavonoid compounds. Besides, silver (Ag)
nanoparticles have been proved to be promising in cancer treatment. Methods: The
synergistic effect of medicinal plant extracts with silver antibacterial
nanoparticles on cancer cell inhibition was investigated. Cytotoxic effect of D.
regia extract with silver NPs on MCF-7 and Panc-1 cancer cells was investigated by
cell viability analysis, morphological changes, apoptosis induction, and TUNEL
assay. Results: Results showed the cells treated with D. regia extract containing
AgNPs exhibited a considerable reduction in viability percent in comparison with
control cells. Analysis of MTT demonstrated that the IC50 value of D. regia extract
and AgNPs on both cell lines observed in 0.5 mg/mL for 24 h. The results
demonstrated that the maximum inhibition of both cell lines growth was observed in
1.5 mg/L and 0.2 mg/L in the presence with D. regia and AgNPs, respectively. Also,
morphological characteristics of cells indicated that D. regia extract and silver
NPs induce cell death by apoptosis and it is confirmed by TUNEL assay. Conclusion:
Interestingly, D. regia extract and Ag NPs had no significant cytotoxicity against
normal cells. It can be concluded that Delonix regia plant and silver nanoparticles
have an inhibitory potential on two cancer cell lines, but in the use of synergism
of D. regia extract and AgNPs, the induction of apoptosis in MCF-7 and Panc-1 was
increased. Interestingly, in normal cells, no significant negative effect was
observed as control. © 2020, Korean Society of Environmental Risk Assessment and
Health Science.
AU - Shameli Rajiri, M.
AU - Aminsalehi, M.
AU - Shahbandeh, M.
AU - Maleki, A.
AU - Jonoubi, P.
AU - Rad, A. C.
DB - Scopus
DO - 10.1007/s13530-020-00067-1
IS - 1
KW - Apoptosis
Cytotoxic
Delonix regia
MCF7
Panc-1
citrate sodium
Delonix regia extract
dimethyl sulfoxide
formazan
penicillin G
plant extract
povidone
silver nanoparticle
streptomycin
trypsin
unclassified drug
apoptosis
Article
cell proliferation
cell viability
controlled study
cytotoxicity
fibroblast
flow cytometry
human
human cell
IC50
MTT assay
pancreas cancer
pancreatic cancer cell line
synergistic effect
TUNEL assay
wrinkle
zeta potential
M3 - Article
PY - 2021
SP - 45-56
ST - Anticancer and therapeutic potential of Delonix regia extract and silver
T2 - Toxicology and Environmental Health Sciences
TI - Anticancer and therapeutic potential of Delonix regia extract and silver
85090061034&doi=10.1007%2fs13530-020-00067-
1&partnerID=40&md5=657eb767b46f95ffa79d32734a2b5f92
VL - 13
ID - 5185
ER -
TY - JOUR
AB - How to avoid the microenvironment limitations in the therapeutic process of
pressure ulcers is still challenging. The development of a functional gel can kill
bacteria and scavenge reactive oxygen species (ROS), which is urgently required in
the therapeutic process of pressure ulcers. Herein, an in situ sprayed gel is
developed with silver nanoparticles (AgNPs) and polydopamine (PDA) NPs (APG) to
obviate microenvironment restrictions in treating pressure ulcers. The gel is
constructed by spraying sodium alginate solution and CaCl2 solution. AgNPs serve as
an antibacterial agent in the formed gel, which can effectively cause bacterial
inactivation and show more than 5 log (>99.999%) bacterial killing efficiency
against methicillin-resistant S. aureus (MRSA), Staphylococcus aureus (S. aureus),
and Escherichia coli (E. coli) in vitro. Meanwhile, PDA NPs serve as the
antioxidative agent in the formed gel, which can facilitate the elimination of ROS
to address the high ROS problem in wound microenvironment. Based on these features,
it is demonstrated through cell and animal experiments that the AgNPs and PDA NPs
incorporated gel can realize the effective treatment of MRSA-infected and hydrogen
peroxide (H2O2)-sensitized pressure ulcers. It is believed that the designed system
by a simple spray-coating approach can provide a new therapeutic strategy in
biomedical areas. © 2023 Wiley-VCH GmbH.
AU - Shan, J.
AU - Li, X.
AU - Huang, Z.
AU - Kong, B.
AU - Wang, H.
AU - Ren, L.
C7 - 2300006
DB - Scopus
DO - 10.1002/mabi.202300006
IS - 5
KW - bacteria
gels
microenvironments
pressure ulcers
ROS
Animals
Escherichia coli
Metal Nanoparticles
Pressure Ulcer
Silver
Chlorine compounds
Diseases
Silver compounds
Sodium alginate
alginic acid
calcium chloride
dermatological agent
dopamine
gel
hydrogen peroxide
silver nanoparticle
silver polydopamine nanoparticle
unclassified drug
antiinfective agent
metal nanoparticle
silver
Alginate solutions
Bacterial inactivation
Difunctional
Methicillin
Microenvironments
Polydopamine
Pressure ulcers
S. aureus
Scavenge reactive oxygen species
animal experiment
animal model
animal tissue
Article
biocompatibility
controlled study
decubitus
in vitro study
in vivo study
inflammatory cell
mouse
nonhuman
scar
spray coating
wound
wound care
wound healing
animal
Gels
M3 - Article
PY - 2023
ST - In Situ Sprayed Difunctional Gel Avoiding Microenvironments Limitations to
Treat Pressure Ulcers
T2 - Macromolecular Bioscience
TI - In Situ Sprayed Difunctional Gel Avoiding Microenvironments Limitations to
Treat Pressure Ulcers
85151617645&doi=10.1002%2fmabi.202300006&partnerID=40&md5=224092ea2e0ef109c53756318
1003ba6
VL - 23
ID - 5012
ER -
TY - JOUR
AB - As the release of silver nanoparticles (AgNPs) in the environment continues
to increase, great concerns have been raised about their potential toxicity to
humans. It is urgent to assess the possible toxicity of AgNPs to the immune cells
of the central nervous system due to the continuous accumulation of AgNPs in the
brain. This study aimed to evaluate the neurotoxicity of AgNPs and the regulatory
mechanism of autophagy in AgNPs-induced inflammation by using mouse microglia BV2
cell lines. AgNPs decreased the microglia cell activity in a concentration and
timedependent manner. The exposure of BV2 cells to AgNPs at a non-cytotoxic level
of 5 mu g/mL resulted in increase of pro-inflammatory cytokines and decrease of
mRNA expression of anti-inflammatory cytokines. AgNPs exposure increased M1 markers
of iNOS expression and decreased the expression of M2 markers of CD206 in a
timedependent manner. Meanwhile, the expression of inflammatory proteins IL-1 beta
and NF-kappa B increased significantly. Additionally, AgNPs induced an increase in
autophagosome and upregulation of LC3II, Beclin1, and p62 expression levels.
Pretreatment by an autophagy inhibitor, 3-Methyladenine, caused more AgNPs-treated
microglia to polarized into pro-inflammatory phenotypes. Inhibition of autophagy
also increased the expression of inflammation-associated mRNA and proteins in BV2
cells. These results indicated that AgNPs could induce pro-inflammatory phenotypic
polarization of microglia and the autophagy could play a key regulatory role in the
pro-inflammatory phenotypic polarization of microglia induced by AgNPs.
AN - WOS:000677525000005
AU - Shang, M. T.
AU - Chang, X. R.
AU - Niu, S. Y.
AU - Li, J. Y.
AU - Zhang, W. L.
AU - Wu, T. S.
AU - Zhang, T.
AU - Tang, M.
AU - Xue, Y. Y.
C6 - JUN 2021
C7 - 112324
DA - AUG
DO - 10.1016/j.fct.2021.112324
PY - 2021
SN - 0278-6915
1873-6351
ST - The key role of autophagy in silver nanoparticle-induced BV2 cells
inflammation and polarization
TI - The key role of autophagy in silver nanoparticle-induced BV2 cells
VL - 154
ID - 6201
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been incorporated in many consumer and
biomedical products. Serious concerns have been expressed about the environmental
and public health risks caused by nanoparticles. In previous studies, we found that
AgNPs induced microglia polarization of the inflammatory phenotype. Autophagy was a
critical for AgNPs-induced neuroinflammation. In the present study, we evaluated in
detail the effects of AgNPs in different stages of the autophagy process, and we
found that AgNPs induced neuroinflammatory responses and autophagic flux blockage
both in the mouse brain and BV2 cells. AgNPs inhibited autophagosome-lysosome
fusion and impaired the lysosomal functions by reducing the levels of
lysosomalassociated membrane proteins, promoting lysosome membrane permeability and
altering the lysosomal acidic microenvironment. These changes resulted in the
defects in autophagic substrate clearance and subsequently led neuroinflammation.
In addition, the elevation of autophagy could prevent the neuroinflammation induced
by AgNPs. As a result, AgNPs hindered autophagic flux by inhibiting autophagosome
fusion with lysosomes, thus aggravating the AgNPs-induced neurotoxicity. These
findings will provide new insights to investigate the molecular mechanisms of
neurotoxicity caused by AgNPs.
AN - WOS:000878779200003
AU - Shang, M. T.
AU - Niu, S. Y.
AU - Chang, X. R.
AU - Li, J. Y.
AU - Zhang, W. L.
AU - Guo, M. H.
AU - Wu, T. S.
AU - Zhang, T.
AU - Tang, M.
AU - Xue, Y. Y.
C6 - OCT 2022
C7 - 113469
DA - DEC
DO - 10.1016/j.fct.2022.113469
PY - 2022
SN - 0278-6915
1873-6351
ST - Silver nanoparticle-induced impaired autophagic flux and lysosomal
dysfunction contribute to the microglia inflammation polarization
TI - Silver nanoparticle-induced impaired autophagic flux and lysosomal
VL - 170
ID - 6415
ER -
TY - JOUR
AB - As the release of silver nanoparticles (AgNPs) in the environment continues
to increase, great concerns have been raised about their potential toxicity to
humans. It is urgent to assess the possible toxicity of AgNPs to the immune cells
of the central nervous system due to the continuous accumulation of AgNPs in the
brain. This study aimed to evaluate the neurotoxicity of AgNPs and the regulatory
mechanism of autophagy in AgNPs-induced inflammation by using mouse microglia BV2
cell lines. AgNPs decreased the microglia cell activity in a concentration and
time-dependent manner. The exposure of BV2 cells to AgNPs at a non-cytotoxic level
of 5 μg/mL resulted in increase of pro-inflammatory cytokines and decrease of mRNA
expression of anti-inflammatory cytokines. AgNPs exposure increased M1 markers of
iNOS expression and decreased the expression of M2 markers of CD206 in a time-
dependent manner. Meanwhile, the expression of inflammatory proteins IL-1β and NF-
κB increased significantly. Additionally, AgNPs induced an increase in
autophagosome and upregulation of LC3II, Beclin1, and p62 expression levels.
Pretreatment by an autophagy inhibitor, 3-Methyladenine, caused more AgNPs-treated
microglia to polarized into pro-inflammatory phenotypes. Inhibition of autophagy
also increased the expression of inflammation-associated mRNA and proteins in BV2
cells. These results indicated that AgNPs could induce pro-inflammatory phenotypic
polarization of microglia and the autophagy could play a key regulatory role in the
pro-inflammatory phenotypic polarization of microglia induced by AgNPs. © 2021
Elsevier Ltd
AU - Shang, M.
AU - Chang, X.
AU - Niu, S.
AU - Li, J.
AU - Zhang, W.
AU - Wu, T.
AU - Zhang, T.
AU - Tang, M.
AU - Xue, Y.
C7 - 112324
DB - Scopus
DO - 10.1016/j.fct.2021.112324
KW - AgNPs
Autophagy
Inflammation
Microglia
Polarization
Animals
Cell Line
Cell Polarity
Metal Nanoparticles
Mice
Silver
beclin 1
interleukin 1beta
peptides and proteins
protein LC3II
sequestosome 1
silver nanoparticle
unclassified drug
metal nanoparticle
silver
animal cell
Article
autophagosome
BV-2 cell line
controlled study
gene expression
mouse
neurotoxicity
nonhuman
polarization
protein expression
upregulation
animal
autophagy
cell line
cell polarity
chemistry
drug effect
immunology
inflammation
microglia
physiology
M3 - Article
PY - 2021
ST - The key role of autophagy in silver nanoparticle-induced BV2 cells
TI - The key role of autophagy in silver nanoparticle-induced BV2 cells
85107699274&doi=10.1016%2fj.fct.2021.112324&partnerID=40&md5=36f70c4be02c26c65b8118
f86adc1f4e
VL - 154
ID - 5253
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been incorporated in many consumer and
biomedical products. Serious concerns have been expressed about the environmental
and public health risks caused by nanoparticles. In previous studies, we found that
AgNPs induced microglia polarization of the inflammatory phenotype. Autophagy was a
critical for AgNPs-induced neuroinflammation. In the present study, we evaluated in
detail the effects of AgNPs in different stages of the autophagy process, and we
found that AgNPs induced neuroinflammatory responses and autophagic flux blockage
both in the mouse brain and BV2 cells. AgNPs inhibited autophagosome-lysosome
fusion and impaired the lysosomal functions by reducing the levels of lysosomal-
associated membrane proteins, promoting lysosome membrane permeability and altering
the lysosomal acidic microenvironment. These changes resulted in the defects in
autophagic substrate clearance and subsequently led neuroinflammation. In addition,
the elevation of autophagy could prevent the neuroinflammation induced by AgNPs. As
a result, AgNPs hindered autophagic flux by inhibiting autophagosome fusion with
lysosomes, thus aggravating the AgNPs-induced neurotoxicity. These findings will
provide new insights to investigate the molecular mechanisms of neurotoxicity
caused by AgNPs. © 2022 Elsevier Ltd
AU - Shang, M.
AU - Niu, S.
AU - Chang, X.
AU - Li, J.
AU - Zhang, W.
AU - Guo, M.
AU - Wu, T.
AU - Zhang, T.
AU - Tang, M.
AU - Xue, Y.
C7 - 113469
DB - Scopus
DO - 10.1016/j.fct.2022.113469
KW - AgNPs
Autophagosome–lysosome fusion
Autophagy
Lysosome
Neuroinflammation
Animals
Inflammation
Lysosomes
Metal Nanoparticles
Mice
Microglia
Silver
4 dimethylaminoazobenzene
acridine orange
CD163 antigen
CD86 antigen
interleukin 1beta
povidone
sequestosome 1
silver nanoparticle
metal nanoparticle
silver
animal cell
animal experiment
Article
autophagosome
BV-2 cell line
cell culture
cell viability
cytotoxicity assay
flow cytometry
histopathology
human
human cell
immunoblotting
immunocytochemistry
immunofluorescence
lysosome
microenvironment
microglia
neurotoxicity
nonhuman
particle size
polarization
Western blotting
animal
autophagy
chemistry
inflammation
metabolism
mouse
M3 - Article
PY - 2022
ST - Silver nanoparticle-induced impaired autophagic flux and lysosomal
TI - Silver nanoparticle-induced impaired autophagic flux and lysosomal
85139864042&doi=10.1016%2fj.fct.2022.113469&partnerID=40&md5=1656e36da3c94e894eee9e
a7c793c785
VL - 170
ID - 4981
ER -
TY - JOUR
AB - Background. Because of its diverse range of use in several ethics of
diagnosis and care of multiple diseases, nanotechnology has seen remarkable growth
and has become a key component of medical sciences. In recent years, there has been
rapid advancement in medicine and biomaterials. Nanomedicine aids in illness
prevention, diagnosis, monitoring, and treatment. Aim. The purpose of this work is
to evaluate the antibacterial, anti-inflammatory, and cytotoxic capabilities of
green produced silver nanoparticle with the addition of curcumin-assisted chitosan
nanocomposite (SCCN) against wound pathogenic as reducing agents. Materials and
Methods. The plant extract of Pongamia pinnata, silver nanoparticles, and its based
curcumin nanoformulations was studied in this study utilizing UV visible
spectrophotometer, selected area electron diffraction (SAED), and TEM. Anti-
inflammatory, antimicrobial, and cytotoxic tests were performed on silver
nanoparticles with the addition of curcumin-assisted chitosan nanocomposite (SCCN).
Furthermore, these produced nanocomposites were coated on clinical silk and tested
for antibacterial activity. Results. The produced silver nanoparticle with the
addition of curcumin-assisted chitosan nanocomposite (SCCN) has significant
antibacterial activities against Pseudomonas aeruginosa and staphylococcus aureus.
They are as well as possess anti-inflammatory activity and furthermore prove to be
biocompatible. Conclusion. This advancement in the field of biomaterials, which
means nanocomposite, not only helps to reduce the harmful effects of pathogenic
organisms while representing an environmentally benign material but it also shows
to be a material with zero danger to humans and the environment. © 2021 Rajeshkumar
Shanmugam et al.
AU - Shanmugam, R.
AU - Subramaniam, R.
AU - Kathirason, S. G.
AU - Ali, D.
AU - Balusamy, S. R.
AU - Gurusamy, A.
AU - Arunachalam, K.
AU - Sellami, H.
C7 - 3091587
DB - Scopus
DO - 10.1155/2021/3091587
KW - Anti-Bacterial Agents
Cell Line, Tumor
Chitosan
Curcumin
Humans
Metal Nanoparticles
Millettia
Nanocomposites
Nanotechnology
Particle Size
Plant Extracts
Pseudomonas Infections
Silver
Wound Healing
biomaterial
curcumin
dimethyl sulfoxide
nanocomposite
plant extract
Pongamia pinnata extract
reducing agent
silk
silver nanoparticle
unclassified drug
antiinfective agent
chitosan
metal nanoparticle
silver
Article
biocompatibility
controlled study
cytotoxicity
cytotoxicity assay
drug formulation
drug synthesis
electron diffraction
herb
infectious agent
kinetics
MTT assay
nanomedicine
nonhuman
Pongamia pinnata
zone of inhibition
chemistry
drug effect
human
nanotechnology
particle size
procedures
Pseudomonas infection
tumor cell line
wound healing
M3 - Article
PY - 2021
ST - Curcumin-Chitosan Nanocomposite Formulation Containing Pongamia pinnata -
Mediated Silver Nanoparticles, Wound Pathogen Control, and Anti-Inflammatory
Potential
TI - Curcumin-Chitosan Nanocomposite Formulation Containing Pongamia pinnata -
Mediated Silver Nanoparticles, Wound Pathogen Control, and Anti-Inflammatory
Potential
85122755124&doi=10.1155%2f2021%2f3091587&partnerID=40&md5=58fc403a650d300b19210a101
f66cc60
VL - 2021
ID - 5245
ER -
TY - JOUR
AB - Nanoparticle (NP) association with macromolecules in a physiological
environment forms a biocorona (BC), which alters NP distribution, activity, and
toxicity. While BC formation is dependent on NP physicochemical properties, little
information exists on the influence of the physiological environment. Obese
individuals and those with cardiovascular disease exist with altered serum
chemistry, which is expected to influence BC formation and NP toxicity. We
hypothesize that a BC formed on NPs following incubation in hyperlipidemic serum
will result in altered NP-BC protein content, cellular association, and toxicity
compared to normal serum conditions. We utilized Fe3O4 NPs, which are being
developed as MRI contrast and tumor targeting agents to test our hypothesis. We
used rat aortic endothelial cells (RAECs) within a dynamic flow in vitro exposure
system to more accurately depict the in vivo environment. A BC was formed on 20 nm
PVP-suspended Fe3O4 NPs following incubation in water, 10% normal or hyperlipidemic
rat serum. Addition of BCs resulted in increased hydrodynamic size and decreased
surface charge. More cholesterol associated with Fe3O4 NPs after incubation in
hyperlipidemic as compared with normal serum. Using quantitative proteomics, we
identified unique differences in BC protein components between the 2 serum types.
Under flow conditions, formation of a BC from both serum types reduced RAECs
association of Fe3O4 NPs. Addition of BCs was found to exacerbate RAECs
inflammatory gene responses to Fe3O4 NPs (Fe3O4-hyperlipidemic > Fe3O4-normal >
Fe3O4) including increased expression of IL-6, TNF-alpha, Cxcl-2, VCAM-1, and ICAM-
1. Overall, these findings demonstrate that disease-induced variations in
physiological environments have a significant impact NP-BC formation, cellular
association, and cell response.
AN - WOS:000385300400016
AU - Fritz, K. S.
AU - Raghavendra, A. J.
AU - Podila, R.
AU - Persaud, I.
AU - Brown, J. M.
DA - AUG
DO - 10.1093/toxsci/kfw097
IS - 2
PY - 2016
SN - 1096-6080
1096-0929
SP - 406-416
ST - Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and
the Toxicological Consequences
TI - Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and
the Toxicological Consequences
VL - 152
ID - 6669
ER -
TY - JOUR
AB - Human inhalation exposures to manufactured nanoparticles (NP) and airborne
ultrafine particles (UFP) continues to increase in both occupational and
environmental settings. UFP exposures have been associated with increased
cardiovascular mortality and morbidity, while ongoing research supports adverse
systemic and cardiovascular health effects after NP exposures. Adverse
cardiovascular health effects include alterations in heart rate variability,
hypertension, thrombosis, arrhythmias, increased myocardial infarction, and
atherosclerosis. Exactly how UFP and NP cause these negative cardiovascular effects
is poorly understood, however a variety of mediators and mechanisms have been
proposed. UFP and NP, as well as their soluble components, are known to
systemically translocate from the lung. Translocated particles could mediate
cardiovascular toxicity through direct interactions with the vasculature, blood,
and heart. Recent study suggests that sensory nerve stimulation within the lung may
also contribute to UFP- and NP-induced acute cardiovascular alterations. Activation
of sensory nerves, such as C-fibers, within the lung may result in altered cardiac
rhythm and function. Lastly, release of pulmonary-derived mediators into systemic
circulation has been proposed to facilitate cardiovascular effects. In general,
these proposed pulmonary-derived mediators include proinflammatory cytokines,
oxidatively modified macromolecules, vasoactive proteins, and prothrombotic
factors. These pulmonary-derived mediators have been postulated to contribute to
the subsequent prothrombotic, atherogenic, and inflammatory effects after exposure.
This review will evaluate the potential contribution of individual mediators and
mechanisms in facilitating cardiopulmonary toxicity following inhalation of UFP and
NP. Lastly, we will appraise the literature and propose a hypothesis regarding the
possible role of mast cells in contributing to these systemic effects. © 2012
Informa Healthcare USA, Inc.
AU - Kodavanti, U. P.
AU - Brown, J. M.
DB - Scopus
DO - 10.3109/08958378.2012.668229
IS - 5
KW - IL-33
LOX-1
Mast cell
Particulate matter
RAGE
advanced glycation end product receptor
beta adrenergic receptor blocking agent
carbon nanotube
cerium oxide
chemokine
cytokine
growth factor
lipid
nanoparticle
polystyrene
silver
titanium dioxide
atherogenesis
autonomic nervous system
cardiopulmonary toxicity
cardiotoxicity
cytokine release
endothelium cell
heart function
heart infarction
heart muscle cell
heart rate variability
heart rhythm
human
lung toxicity
mast cell
mediator release
nerve fiber
nonhuman
oxidative stress
particle size
priority journal
review
sensory nerve
thrombosis
ultrafine particle
M3 - Review
PY - 2012
SP - 320-339
ST - Manufactured and airborne nanoparticle cardiopulmonary interactions: A review
of mechanisms and the possible contribution of mast cells
T2 - Inhalation Toxicology
TI - Manufactured and airborne nanoparticle cardiopulmonary interactions: A review
of mechanisms and the possible contribution of mast cells
84859617027&doi=10.3109%2f08958378.2012.668229&partnerID=40&md5=cb980328178049f8620
3cd9f13c50208
VL - 24
ID - 5666
ER -
TY - JOUR
AB - Bacterial infection caused by biomaterials is a very serious problem in the
clinical treatment of implants. The emergence of antibiotic resistance has prompted
other antibacterial agents to replace traditional antibiotics. Silver is rapidly
developing as an antibacterial candidate material to inhibit bone infections due to
its significant advantages such as high antibacterial timeliness, high
antibacterial efficiency, and less susceptibility to bacterial resistance. However,
silver has strong cytotoxicity, which can cause inflammatory reactions and
oxidative stress, thereby destroying tissue regeneration, making the application of
silver-containing biomaterials extremely challenging. In this paper, the
application of silver in biomaterials is reviewed, focusing on the following three
issues: 1) how to ensure the excellent antibacterial properties of silver, and not
easy to cause bacterial resistance; 2) how to choose the appropriate method to
combine silver with biomaterials; 3) how to make silver-containing biomaterials in
hard tissue implants have further research. Following a brief introduction, the
discussion focuses on the application of silver-containing biomaterials, with an
emphasis on the effects of silver on the physicochemical properties, structural
properties, and biological properties of biomaterials. Finally, the review
concludes with the authors' perspectives on the challenges and future directions of
silver in commercialization and in-depth research.
AN - WOS:001011075300001
AU - Shao, H. F.
AU - Zhang, T.
AU - Gong, Y. P.
AU - He, Y.
C6 - JUN 2023
DA - 2023 JUN 20
DO - 10.1002/adhm.202300932
PY - 2023
SN - 2192-2640
2192-2659
ST - Silver-Containing Biomaterials for Biomedical Hard Tissue Implants
TI - Silver-Containing Biomaterials for Biomedical Hard Tissue Implants
ID - 6188
ER -
TY - JOUR
AB - Bacterial infection caused by biomaterials is a very serious problem in the
clinical treatment of implants. The emergence of antibiotic resistance has prompted
other antibacterial agents to replace traditional antibiotics. Silver is rapidly
developing as an antibacterial candidate material to inhibit bone infections due to
its significant advantages such as high antibacterial timeliness, high
antibacterial efficiency, and less susceptibility to bacterial resistance. However,
silver has strong cytotoxicity, which can cause inflammatory reactions and
oxidative stress, thereby destroying tissue regeneration, making the application of
silver-containing biomaterials extremely challenging. In this paper, the
application of silver in biomaterials is reviewed, focusing on the following three
issues: 1) how to ensure the excellent antibacterial properties of silver, and not
easy to cause bacterial resistance; 2) how to choose the appropriate method to
combine silver with biomaterials; 3) how to make silver-containing biomaterials in
hard tissue implants have further research. Following a brief introduction, the
discussion focuses on the application of silver-containing biomaterials, with an
emphasis on the effects of silver on the physicochemical properties, structural
properties, and biological properties of biomaterials. Finally, the review
concludes with the authors’ perspectives on the challenges and future directions of
silver in commercialization and in-depth research. © 2023 Wiley-VCH GmbH.
AU - Shao, H.
AU - Zhang, T.
AU - Gong, Y.
AU - He, Y.
DB - Scopus
DO - 10.1002/adhm.202300932
KW - antibacterials
biomaterials
coatings
doping
silver
Antibiotics
Tissue
Tissue regeneration
Antibacterials
Antibiotics resistance
Bacterial resistance
Bone infection
Candidate materials
Clinical treatments
Hard tissue implants
Silver
M3 - Review
PY - 2023
ST - Silver-Containing Biomaterials for Biomedical Hard Tissue Implants
TI - Silver-Containing Biomaterials for Biomedical Hard Tissue Implants
85162160976&doi=10.1002%2fadhm.202300932&partnerID=40&md5=04fb3d54bf53fbce3daf530c4
5f4f377
ID - 5033
ER -
TY - JOUR
AB - Objective: To explore the therapeutic effect and mechanism of Qiling Tongluo
prescription against idiopathic membranous nephropathy (IMN) in rats based on Toll-
like receptor 4/myeloid differentiation factor 88/nuclear transcription factor-κB
(TLR4/MyD88/NF-κB) signaling pathway. Method: Sixty male SD rats were randomly
divided into the normal group, model group, benazepril hydrochloride (10 mg·kg-1)
group, and low-, medium-, and high-dose (6.48, 12.95, and 25.9 g·kg-1) Qiling
Tongluo prescription groups. The IMN rat model was established by injection of
cationized bovine serum albumin (C-BSA) into the tail vein. After the model was
successfully prepared, the rats were gavaged with the corresponding drugs, once a
day, for four consecutive weeks. After the treatment, the pathological changes in
rat kidneys were observed by hematoxylineosin (HE) staining, Masson staining, and
periodic acid-silver metheramine (PASM) staining, followed by the detection of 24 h
urinary total protein (24 h UTP), plasma albumin (ALB), total serum protein (TP),
serum creatinine (SCr), urea nitrogen (BUN), and uric acid (UA) levels. The levels
of interleukin-1β (IL-1β) and interleukin-6 (IL-6) were determined by enzyme-linked
immunosorbent assay (ELISA), and the mRNA and protein expression levels of TLR4,
MyD88, and NF-κB in the kidney tissue were assayed by real-time fluorescent
quantitative polymerase chain reaction (Real-time PCR), immunohistochemistry (IHC),
and Western blot. Result: Compared with the normal group, the model group exhibited
elevated 24 h UTP and serum SCr, BUN, UA, IL-1β, and IL-6 (P<0.05, P<0.01),
decreased ALB and TP (P<0.01), up-regulated TLR4, MyD88, and NF-κB p65 mRNA and
protein expression in kidney tissue (P<0.05, P<0.01), obvious inflammation,
disordered glomerular structure with enlarged volume, irregularly thickened
basement membrane, inflammatory cell infiltration in the renal interstitium,
reduced renal tubular epithelial cells due to shedding and apoptosis, and some
vacuolar degeneration. Compared with the model group, benazepril hydrochloride and
Qiling Tongluo prescription at the high dose remarkably lowered the serum SCr and
UA (P<0.05) and increased ALB and TP (P<0.05). Benazepril hydrochloride and Qiling
Tongluo prescription at the low, medium, and high doses down-regulated the 24 h
UTP, serum IL-1β and IL-6 levels, and renal TLR4, MyD88, and NF-κB p65 mRNA and
protein expression to varying degrees (P<0.05, P<0.01), alleviated IMN inflammatory
reaction, glomerular swelling, and volume increase, slightly dilated glomerular
capillaries, proliferated mesangial matrix, and relieved pathological and
morphological damages in rat kidney, with inflammatory cell infiltration
occasionally observed. Conclusion: Qiling Tongluo prescription may reduce the
release and expression of inflammatory factors by regulating the TLR4/MyD88/NF-κB
signaling pathway to inhibit the inflammatory response in IMN rats, ameliorate
proteinuria and kidney damage, and protect kidney function. © 2022, China Academy
of Chinese Medical Sciences Institute of Chinese Materia Medica. All rights
reserved.
AU - Shao, J. B.
AU - Wang, X.
AU - Xu, H. Z.
AU - Gao, F.
AU - Zhang, Y. Q.
AU - Zhang, Y. H.
AU - Yuan, M. H.
AU - Pan, L.
DB - Scopus
DO - 10.13422/j.cnki.syfjx.20220391
IS - 4
KW - Idiopathic membranous nephropathy (IMN)
Myeloid differentiation factor 88 (MyD88)
Nuclear transcription factor-κB (NF-κB)
Qiling Tongluo prescription
Toll-like receptor 4 (TLR4)
albumin
benazepril
Chinese drug
creatinine
interleukin 1beta
interleukin 6
nitrogen
qiling tongluo
unclassified drug
uric acid
animal experiment
animal model
animal tissue
apoptosis
Article
cell infiltration
Chinese medicine
controlled study
drug activity
drug efficacy
drug megadose
gene expression
inflammation
kidney injury
kidney tissue
low drug dose
male
membranous glomerulonephritis
morphological trait
NF kB signaling
nonhuman
prescription
protein expression
rat
Western blotting
M3 - Article
PY - 2022
SP - 100-108
ST - Qiling Tongluo Prescription Improves Renal Injury in Rats with Idiopathic
Membranous Nephropathy by Inhibiting TLR4/MyD88/NF-κB Signaling Pathway
T2 - Chinese Journal of Experimental Traditional Medical Formulae
TI - Qiling Tongluo Prescription Improves Renal Injury in Rats with Idiopathic
Membranous Nephropathy by Inhibiting TLR4/MyD88/NF-κB Signaling Pathway
85131522671&doi=10.13422%2fj.cnki.syfjx.20220391&partnerID=40&md5=69f4638cc932b609e
b95e25d97f4a298
VL - 28
ID - 5124
ER -
TY - JOUR
AB - Aim: To evaluate the antibacterial potential and biological performance of
silver nanoparticles in chitosanbased membranes. Materials & methods: Electrospun
chitosan/poly(ethylene oxide) membranes with different amounts of silver
nanoparticles were evaluated for antibacterial properties and cytotoxicity in vitro
and for tissue response in a rabbit subcutaneous model. Results: The nanoparticles
displayed dose-dependent antibacterial properties against Porphyromonas gingivalis
and Fusobacterium nucleatum, without showing noticeable cytotoxicity. The membranes
with silver nanoparticles evoked a similar inflammatory response compared with the
membranes without silver nanoparticles. Conclusion: The antibacterial effect,
combined with the findings on cyto- and biocompatibility warrants further
investigation to the usefulness of chitosan/poly(ethylene oxide) membranes with
silver nanoparticles, for clinical applications like guided tissue regeneration.
AN - WOS:000417038800007
AU - Shao, J. L.
AU - Yu, N.
AU - Kolwijck, E.
AU - Wang, B.
AU - Tan, K. W.
AU - Jansen, J. A.
AU - Walboomers, X. F.
AU - Yang, F.
DA - NOV
DO - 10.2217/nnm-2017-0172
IS - 22
PY - 2017
SN - 1743-5889
1748-6963
SP - 2771-2785
ST - Biological evaluation of silver nanoparticles incorporated into chitosan-
based membranes
T2 - NANOMEDICINE
TI - Biological evaluation of silver nanoparticles incorporated into chitosan-
based membranes
VL - 12
ID - 5848
ER -
TY - JOUR
AB - Aim: To evaluate the antibacterial potential and biological performance of
silver nanoparticles in chitosan-based membranes. Materials & methods: Electrospun
chitosan/poly(ethylene oxide) membranes with different amounts of silver
nanoparticles were evaluated for antibacterial properties and cytotoxicity in vitro
and for tissue response in a rabbit subcutaneous model. Results: The nanoparticles
displayed dose-dependent antibacterial properties against Porphyromonas gingivalis
and Fusobacterium nucleatum, without showing noticeable cytotoxicity. The membranes
with silver nanoparticles evoked a similar inflammatory response compared with the
membranes without silver nanoparticles. Conclusion: The antibacterial effect,
combined with the findings on cyto- and biocompatibility warrants further
investigation to the usefulness of chitosan/poly(ethylene oxide) membranes with
silver nanoparticles, for clinical applications like guided tissue regeneration. ©
2017 Future Medicine Ltd.
AU - Shao, J.
AU - Yu, N.
AU - Kolwijck, E.
AU - Wang, B.
AU - Tan, K. W.
AU - Jansen, J. A.
AU - Walboomers, X. F.
AU - Yang, F.
DB - Scopus
DO - 10.2217/nnm-2017-0172
IS - 22
KW - antibacterial
biocompatibility
chitosan
electrospinning
Animals
Cell Line
Cell Survival
Chitosan
Fusobacterium nucleatum
Humans
Hydrogels
Male
Metal Nanoparticles
Particle Size
Rabbits
Silver
Silver Compounds
chemokine receptor CCR1
chemokine receptor CCR5
chemokine receptor CX3CR1
interleukin 10
interleukin 17
interleukin 18
interleukin 6
macrogol
silver nanoparticle
antiinfective agent
biomaterial
macrogol derivative
metal nanoparticle
SilvaSorb
silver
silver derivative
animal experiment
animal tissue
Article
biological activity
cell viability
controlled study
cytotoxicity
human
human cell
human cell culture
in vitro study
in vivo study
inflammation
male
membrane
nonhuman
priority journal
protein expression
tissue reaction
animal
artificial membrane
cell line
cell survival
chemistry
drug effect
hydrogel
Leporidae
particle size
pharmacology
M3 - Article
PY - 2017
SP - 2771-2785
ST - Biological evaluation of silver nanoparticles incorporated into chitosan-
based membranes
T2 - Nanomedicine
TI - Biological evaluation of silver nanoparticles incorporated into chitosan-
based membranes
85032435198&doi=10.2217%2fnnm-2017-
0172&partnerID=40&md5=d4049755bc8dbacc4ca8ceb251dca78a
VL - 12
ID - 5541
ER -
TY - JOUR
AB - An ideal tissue-engineered dermal substitute should possess angiogenesis
potential to promote wound healing, antibacterial activity to relieve the bacterial
burden on skin, as well as sufficient porosity for air and moisture exchange. In
light of this, a glass–ceramic (GC) has been incorporated into chitosan and gelatin
electrospun nanofibers (240–360 nm), which MEFs were loaded on it for healing
acceleration. The GC was doped with silver to improve the antibacterial activity.
The bioactive nanofibrous scaffolds demonstrated antibacterial and superior
antibiofilm activities against Gram-negative and Gram-positive bacteria. The
nanofibrous scaffolds were biocompatible, hemocompatible, and promoted cell
attachment and proliferation. Nanofibrous skin substitutes with or without Ag-doped
GC nanoparticles did not induce an inflammatory response and attenuated LPS-induced
interleukin-6 release by dendritic cells. The rate of biodegradation of the
nanocomposite was similar to the rate of skin regeneration under in vivo
conditions. Histopathological evaluation of full-thickness excisional wounds in
BALB/c mice treated with mouse embryonic fibroblasts-loaded nanofibrous scaffolds
showed enhanced angiogenesis, and collagen synthesis as well as regeneration of the
sebaceous glands and hair follicles in vivo. © 2022 The Authors. Bioengineering &
Translational Medicine published by Wiley Periodicals LLC on behalf of American
Institute of Chemical Engineers.
AU - Sharifi, E.
AU - Sadati, S. A.
AU - Yousefiasl, S.
AU - Sartorius, R.
AU - Zafari, M.
AU - Rezakhani, L.
AU - Alizadeh, M.
AU - Nazarzadeh Zare, E.
AU - Omidghaemi, S.
AU - Ghanavatinejad, F.
AU - Jami, M. S.
AU - Salahinejad, E.
AU - Samadian, H.
AU - Paiva-Santos, A. C.
AU - De Berardinis, P.
AU - Shafiee, A.
AU - Tay, F. R.
AU - Pourmotabed, S.
AU - Makvandi, P.
DB - Scopus
DO - 10.1002/btm2.10386
KW - Ag-doped bioactive glass–ceramics
anti-biofilm
hemocompatible
immunogenicity
skin substitute
wound healing
Bacteria
Bioactive glass
Biocompatibility
Biodegradation
Cell culture
Mammals
Nanofibers
Scaffolds (biology)
Silver compounds
Tissue regeneration
Ag doped
Ag-doped bioactive glass–ceramic
Antibiofilms
Bioactive glass ceramics
Glass-ceramics
Hemocompatible
Immunogenicity
Nanofibrous scaffolds
Skin substitutes
Wound healing
Nanoparticles
M3 - Article
PY - 2022
ST - Cell loaded hydrogel containing Ag-doped bioactive glass–ceramic
nanoparticles as skin substitute: Antibacterial properties, immune response, and
scarless cutaneous wound regeneration
T2 - Bioengineering and Translational Medicine
TI - Cell loaded hydrogel containing Ag-doped bioactive glass–ceramic
nanoparticles as skin substitute: Antibacterial properties, immune response, and
scarless cutaneous wound regeneration
85135233633&doi=10.1002%2fbtm2.10386&partnerID=40&md5=72880974088a93161ecde5b885d0c
0ff
ID - 5114
ER -
TY - JOUR
AB - In this present study, the endophytic bacteria were isolated from the
drought-tolerant ornamental plant Pennisetum setaceum. The biomass of endophytic
bacteria was utilized for the biogenic synthesis of silver nanoparticles (AgNPs).
The synthesis of AgNPs was confirmed by UV-Visible and FTIR spectroscopy followed
by SEM analysis. The antibacterial studies were performed through MIC, MBC, and
biofilm assays. Efficacy of AgNPs against the human breast cancer (MCF-7) cells was
also tested, and the IC50 was determined by MTT assay. In our study, we have
observed that the synthesized AgNPs exhibited a dose-dependent cytotoxicity (1–100
μg/mL) against MCF-7 cells and morphological alterations of the cells were also
visualized and the IC50 was observed at 50 μg/mL. The treatment of synthesized
AgNPs altered the expression of apoptotic proteins including Bax, Bcl-2, and
inflammatory marker COX-2 in MCF-7 cells. To the best of our knowledge, this is the
first report that demonstrates the AgNPs from endophytic bacteria isolated from the
plant Pennisetum setaceum can induce apoptosis in human breast cancer MCF-7 cells.
Our results suggest that AgNPs used in this study can be utilized to control human
pathogens and can also be utilized to induce apoptosis in breast cancer cells. ©
2019, Springer-Verlag GmbH Germany, part of Springer Nature.
AU - Shariq Ahmed, M.
AU - Soundhararajan, R.
AU - Akther, T.
AU - Kashif, M.
AU - Khan, J.
AU - Waseem, M.
AU - Srinivasan, H.
DB - Scopus
DO - 10.1007/s11356-019-05869-6
IS - 26
KW - Apoptosis
Bax
Bcl-2
Endophytic bacteria
MCF-7 cells
Bacteria
Cyclooxygenase 2
Endophytes
Humans
MCF-7 Cells
Metal Nanoparticles
Pennisetum
Silver
Pennisetum setaceum
antiinfective agent
BCL2 protein, human
cyclooxygenase 2
metal nanoparticle
protein bcl 2
PTGS2 protein, human
silver
apoptosis
bacterium
biofilm
biogenic emission
biomass
cancer
cell component
detection method
dicotyledon
induced response
iron nanoparticle
morphology
nanoparticle
toxicity
biosynthesis
chemistry
dose response
drug effect
endophyte
human
MCF-7 cell line
metabolism
microbiology
M3 - Article
PY - 2019
SP - 26939-26946
ST - Biogenic AgNPs synthesized via endophytic bacteria and its biological
applications
TI - Biogenic AgNPs synthesized via endophytic bacteria and its biological
applications
85069002954&doi=10.1007%2fs11356-019-05869-
6&partnerID=40&md5=66837483b9daa6b671bcf96edd58df37
VL - 26
ID - 5440
ER -
TY - JOUR
AB - Reductive evolution has endowed Mycobacterium tuberculosis (M. tb) with
moonlighting in protein functions. We demonstrate that RipA (Rv1477), a
peptidoglycan hydrolase, activates the NF kappa B signaling pathway and elicits the
production of pro-inflammatory cytokines, TNF-alpha, IL-6, and IL-12, through the
activation of an innate immune-receptor, toll-like receptor (TLR)4. RipA also
induces an enhanced expression of macrophage activation markers MHC-II, CD80, and
CD86, suggestive of M1 polarization. RipA harbors LC3 (Microtubule-associated
protein 1A/1B-light chain 3) motifs known to be involved in autophagy regulation
and indeed alters the levels of autophagy markers LC3BII and P62/SQSTM1
(Sequestosome-1), along with an increase in the ratio of P62/Beclin1, a hallmark of
autophagy inhibition. The use of pharmacological agents, rapamycin and bafilomycin
A1, reveals that RipA activates PI3K-AKT-mTORC1 signaling cascade that ultimately
culminates in the inhibition of autophagy initiating kinase ULK1 (Unc-51 like
autophagy activating kinase). This inhibition of autophagy translates into
efficient intracellular survival, within macrophages, of recombinant Mycobacterium
smegmatis expressing M. tb RipA. RipA, which also localizes into mitochondria,
inhibits the production of oxidative phosphorylation enzymes to promote a Warburg-
like phenotype in macrophages that favors bacterial replication. Furthermore, RipA
also inhibited caspase-dependent programed cell death in macrophages, thus
hindering an efficient innate antibacterial response. Collectively, our results
highlight the role of an endopeptidase to create a permissive replication niche in
host cells by inducing the repression of autophagy and apoptosis, along with
metabolic reprogramming, and pointing to the role of RipA in disease pathogenesis.
AN - WOS:000629997500001
AU - Shariq, M.
AU - Quadir, N.
AU - Sharma, N.
AU - Singh, J.
AU - Sheikh, J. A.
AU - Khubaib, M.
AU - Hasnain, S. E.
AU - Ehtesham, N. Z.
C7 - 636644
DA - MAR 4
DO - 10.3389/fimmu.2021.636644
PY - 2021
SN - 1664-3224
ST - Mycobacterium tuberculosis RipA Dampens TLR4-Mediated Host Protective
Response Using a Multi-Pronged Approach Involving Autophagy, Apoptosis, Metabolic
Repurposing, and Immune Modulation
TI - Mycobacterium tuberculosis RipA Dampens TLR4-Mediated Host Protective
Response Using a Multi-Pronged Approach Involving Autophagy, Apoptosis, Metabolic
Repurposing, and Immune Modulation
VL - 12
ID - 6691
ER -
TY - JOUR
AB - Airborne particulate matter (PM) was sampled by use of an electrostatic
sampler in an oven hall and a receiving hall in a waste-incineration energy plant,
and from urban air in a heavy-traffic street and from background air in Copenhagen.
PM was sampled for 1-2 weeks, four samples at each site. The samples were extracted
and examined for mutagenicity in Salmonella typhimurium strains TA98, YG1041 and
YG5161, for content of inorganic elements and for the presence of eight polycyclic
aromatic hydrocarbons. The induction of IL-6 and IL-8 mRNA expression and the
presence of DNA damage - tested by the comet assay - were determined after 24-h
incubations with human A549 lung epithelial cells. The PM2.5 concentration was
about twofold greater in the oven hall than in the receiving hall. The particle
size distribution in the receiving hall was similar to that in street air (maximum
mode at about 25 nm), but the distribution was completely different in the oven
hall (maximum mode at about 150 nm). Also chemically, the samples from the oven
hall were highly different from the other samples. PM extracts from the receiving
hall, street and background air were more mutagenic than the PM extracts from the
oven hall. PM from all four sites caused similar levels of DNA damage in A549
cells; only the oven hall samples gave results that were statistically
significantly different from those obtained with street-air samples. The receiving
hall and the urban air samples were similarly inflammatory (relative IL-8 mRNA
expression), whereas the oven hall did not cause a statistically significant
increase in IL-8 mRNA expression. A principal component analysis separated the oven
hall and the receiving hall by the first principal component. These two sites were
separated from street and background air with the second principal component.
Several clusters of constituents were identified. One cluster consisted of all the
polycyclic aromatic hydrocarbons (PAH), several groups of metals and one group of
the biological endpoints (DNA damage, IL-6 and IL-8 mRNA expression). The PAH and
the inorganic content of the air in the receiving hall may be due to vehicle
emissions and suspended waste particles. The inorganic content in the street and
background air may have been influenced by break wear, road emissions and long-
range transport. The results from a partial least-square regression analysis
predicted that both PAHs and a group of metals including Fe and Mn contributed to
IL-6 and IL-8 induction. Only Mn and Sr were predicted to influence DNA damage
statistically significantly. Among the PAHs only chrysene had influence on DNA
damage. The PM from the oven hall was markedly different from the PM at other
locations in particle size distribution, chemical composition and the resulting
biological effects when A549 cells were incubated with the PM. These
characteristics and observations in the oven hall indicated that the PM source was
oven exhaust, which was well combusted. © 2007 Elsevier B.V. All rights reserved.
AU - Sharma, A. K.
AU - Jensen, K. A.
AU - Rank, J.
AU - White, P. A.
AU - Lundstedt, S.
AU - Gagne, R.
AU - Kristiansen, J.
AU - Vogel, U.
AU - Wallin, H.
DB - Scopus
DO - 10.1016/j.mrgentox.2007.05.013
IS - 2
KW - Chemical composition
Genotoxicity
Incineration energy plant
Inflammation
Particle size distribution
Urban air
Air Pollutants
Comet Assay
Humans
Incineration
Inorganic Chemicals
Metals
Mutagenicity Tests
Particle Size
Particulate Matter
Polycyclic Hydrocarbons, Aromatic
aluminum
barium
boron
cadmium
calcium
chromium
copper
DNA
inorganic compound
interleukin 6
interleukin 8
iron
lead
magnesium
manganese
messenger RNA
molybdenum
nickel
phosphorus
potassium
silicon
silver
sodium
strontium
tin
titanium
vanadium
zinc
zirconium
air sampling
airborne particle
article
comet assay
controlled study
cytotoxicity
DNA damage
epithelium cell
frameshift mutation
genotoxicity
human
human cell
incineration
inflammation
mutagenicity
partial least squares regression
particle size
priority journal
Salmonella
statistical significance
M3 - Article
PY - 2007
SP - 95-111
ST - Genotoxicity, inflammation and physico-chemical properties of fine particle
samples from an incineration energy plant and urban air
T2 - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
TI - Genotoxicity, inflammation and physico-chemical properties of fine particle
samples from an incineration energy plant and urban air
34547828295&doi=10.1016%2fj.mrgentox.2007.05.013&partnerID=40&md5=3eac935774ba4fa87
19b700dbfc7a368
VL - 633
ID - 5824
ER -
TY - JOUR
AB - The biogenic tailoring of silver nanoparticles using plant extract is
becoming an attractive approach in the current scenario. Manilkara zapota (MZ) is
well known for its antibacterial, hepato-protective, anti-inflammatory, anti-
tussive, anti-fungal, anti-tumour, and free radical scavenging potential. Its
plants extract is a rich source of secondary metabolites. Nowadays, silver
nanoparticles (AgNPs) have been advocated for a variety of biomedical applications.
In present work, silver nanoparticles have been synthesized using an aqueous
extract of MZ, physicochemically characterized and finally evaluated for
antimicrobial effects, catalytic reduction/degradation of organic dyes and
cytotoxicity. The nanosized AgNPs (~ 84 nm) were found to possess prominent
antibacterial potential against gram positive and gram negative pathogens (MIC
50 μg/ml) in comparison to native plant extract. Moreover, these particles were
found to be non-toxic and efficient eradicators of environmental toxicants via
rapid catalytic reduction of toxic chemicals and dyes. Altogether, these results
suggest promising potential of these nanoparticles that can be used as
multifunctional agents for future biomedical applications. © 2020, Association of
Microbiologists of India.
AU - Sharma, B.
AU - Singh, I.
AU - Bajar, S.
AU - Gupta, S.
AU - Gautam, H.
AU - Kumar, P.
DB - Scopus
DO - 10.1007/s12088-020-00889-0
IS - 4
KW - Antibacterial
Antibiofilm
Antioxidant activity
Catalytic reduction
Manilkara zapota
Phytonanotechnology
silver nanoparticle
animal cell
antifungal activity
antitussive activity
Article
bioassay
biogenesis
biological activity
catalysis
cellular parameters
controlled study
cytocompatibility
cytotoxicity
disk diffusion
drug degradation
drug synthesis
human
human cell
microtitre plate assay
nonhuman
physical chemistry
Raman spectrometry
X ray diffraction
zone of inhibition
M3 - Article
PY - 2020
SP - 468-474
ST - Biogenic Silver Nanoparticles: Evaluation of Their Biological and Catalytic
Potential
T2 - Indian Journal of Microbiology
TI - Biogenic Silver Nanoparticles: Evaluation of Their Biological and Catalytic
Potential
85085361331&doi=10.1007%2fs12088-020-00889-
0&partnerID=40&md5=d2bf4abeefcf3a3de816b1fbe620fdef
VL - 60
ID - 5356
ER -
TY - CHAP
AB - With the growing use of nanomaterials in bioapplications, the nanotoxicity of
new nanomaterials has become a safety concern when used in various applications. In
this chapter, technical developments on carbon nanotubes are described including a
historical account, experimental models and potential bioapplications. Carbon
nanotube (CNT) materials display superior properties in electric current carrying
capacity, thermal conductivity, and thermal stability. Due to the unique CNT
structure with high-aspect ratio, CNT may show unusual toxicity and complicate its
safe use in a target tissue. To test nanotoxicity of CNT, we describe a set of
protocols of prior knowledgebased physical and chemical characteristics to develop
3-dimensional in vitro models of the intact skin, and a 3D in vitro model of the
human airway using a co-culture of normal human bronchial epithelial cells and
normal human fibroblasts. The human airway 3D model served as a tool of health risk
assessment of CNTs on the human respiratory systems. To test functionality at
different CNT concentrations in a 3D model, physical characteristics of multiwalled
CNTs and production of nitric oxide (NO) served as cell viability and inflammatory
marker; mitochondrial activity (MTT assay) served as the cytotoxic response of the
epithelial cell layers; transepithelial electrical resistance (TER) measured
nanotoxicity in the changes in airway physiological function. Cytoxicity and
inflammatory responses of CNTs were dependent on different size, mass, shape, and
functionality of CNTs as viable in vivo tests were conducted to evaluate the
toxicity of engineered CNTs. We monitored the transport across skin, and the
physiological perturbation of transepithelial electrical resistance (TER) during
the exposure of different concentrations of CNTs. The mechanisms of CNTs' toxicity
are closely related to their structure, functional group, and surface charge on the
molecule. We established the nanoscale toxicity of fullerenes of CNTs. © 2013 by
Scrivener Publishing LLC. All rights reserved.
AU - Sharma, R.
AU - Kwon, S.
DB - Scopus
DO - 10.1002/9781118644591.ch3
KW - Carbon nanotube
Cytotoxicity
Drug carrier
Fullerene
Nanotoxicity
PY - 2013
SP - 99-144
ST - Carbon Nanotubes: Nanotoxicity Testing and Bioapplications
T2 - Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering
TI - Carbon Nanotubes: Nanotoxicity Testing and Bioapplications
84887144826&doi=10.1002%2f9781118644591.ch3&partnerID=40&md5=1331ee2a08fedbb66984c4
c110669575
ID - 5745
ER -
TY - JOUR
AB - p-CA is a naturally occurring phenolic acid present in most plants and in all
commonly consumed vegetables and fruits. Here we demonstrated the anti-cancer
effect of the food borne phytochemical p-CA both in vitro and in vivo models of
colon cancer using growth rate and tumor incidence as endpoints. Glucose regulated
protein (GRP78) induction and UPR activation plays a key role in oncogenic
progression, therefore increased dependence of cancer cells on these UPR signaling
pathways for survival can be exploited for anti-cancer research. Hence we
investigated the effect of p-CA on Grp78 a molecular chaperone often upregulated in
colon cancer and its impact on unfolded protein response (UPR). Administration of
the procarcinogen 1,2- dimethylhydrazine (DMH) causes Grp78 upregulation and tumor
adaptation via UPR activation. The adaptive activity of UPR activates antiapoptotic
NF-κB that results in upregulation of the markers of inflammation and angiogenesis.
Supplementation of p-CA downregulated Grp78 and activated UPR mediated apoptosis
both in in vitro and in vivo models of colon cancer. Further we observed that p-CA
significantly reduced inflammation by decreasing the expression of cytokines COX-2,
IL-6, TNF-α and PGE2 as analyzed by q-PCR and also reduced the expression of p-p65
and p-IκBα as analyzed by western blot. Further mechanistic insights revealed that
p-CA inhibits Grp78 upregulation in cancer cells through activation of PERK-eIF2α-
ATF-4-CHOP pathway that culminates in apoptosis inducing effect of p-CA. © 2018
Elsevier B.V.
AU - Sharma, S. H.
AU - Rajamanickam, V.
AU - Nagarajan, S.
DB - Scopus
DO - 10.1016/j.cbi.2018.06.001
KW - Apoptosis
Cancer chemoprevention
Colon cancer
ER stress
Grp78 (78 kDa glucose regulated protein)
p-Coumaric acid
Animals
Cell Line, Tumor
Cell Proliferation
Colon
Colonic Neoplasms
Down-Regulation
Heat-Shock Proteins
Humans
Male
Propionates
Rats, Wistar
Signal Transduction
Silver Nitrate
Tumor Burden
Unfolded Protein Response
activating transcription factor 4
cyclooxygenase 2
glucose regulated protein 78
initiation factor 2alpha
interleukin 6
para coumaric acid
prostaglandin E2
protein kinase R like ER kinase
protein serine threonine kinase
unclassified drug
heat shock protein
molecular chaperone GRP78
propionic acid derivative
silver nitrate
trans-3-(4'-hydroxyphenyl)-2-propenoic acid
vasculotropin A
animal experiment
animal model
animal tissue
apoptosis
Article
cancer growth
cell proliferation
colon cancer
colon cancer cell line
controlled study
down regulation
drug targeting
human
human cell
IC50
in vitro study
in vivo study
male
nonhuman
protein expression
rat
unfolded protein response
upregulation
animal
colon
colon tumor
drug effect
metabolism
pathology
signal transduction
tumor cell line
tumor volume
vascularization
Wistar rat
M3 - Article
PY - 2018
SP - 16-28
ST - Antiproliferative effect of p-Coumaric acid targets UPR activation by
downregulating Grp78 in colon cancer
TI - Antiproliferative effect of p-Coumaric acid targets UPR activation by
downregulating Grp78 in colon cancer
85048129579&doi=10.1016%2fj.cbi.2018.06.001&partnerID=40&md5=b6e71504f63d2370ae6b06
27e58891aa
VL - 291
ID - 5410
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) have various pharmaceutical and biomedical
applications owing to their unique physicochemical properties. Zinc (Zn) is an
essential trace element, a strong antioxidant, and has a primary role in gene
expression, enzymatic reactions, and protein synthesis. The present study aims to
explore the toxic effects of Ag-NPs (50 nm) on the liver and kidney of rats and
also to evaluate the potential protective effect of Zn-NPs (100 nm) against these
adverse effects. Forty adult Sprague-Dawley rats were randomly divided into four
equal groups: control group, Ag-NPs group, Zn-NPs group, and Ag-NPs + Zn-NPs group.
Ag-NPs (50 mg/kg) and/or Zn-NPs (30 mg/kg) were administered daily by gavage for 90
days. The results showed that exposure to Ag-NPs increased serum ALT, AST, urea,
and creatinine. Ag-NPs also induced oxidative stress and lipid peroxidation and
increased inflammatory cytokines in hepatic and renal tissues. Moreover,
histopathological and immunohistochemical examinations revealed various
histological alterations and positive caspase-3 expressions in the liver and kidney
following exposure to Ag-NPs. On the other hand, most of these toxic effects were
ameliorated by co-administration of Zn-NPs. It was concluded that Ag-NPs have
hepatotoxic and nephrotoxic effects in rats via different mechanisms including
oxidative stress, inflammation, and apoptosis and that Zn-NPs can be used to
alleviate these harmful effects by their antioxidative, anti-inflammatory, and
antiapoptotic properties.
AN - WOS:000640141100001
AU - Shehata, A. M.
AU - Salem, F. M. S.
AU - El-Saied, E. M.
AU - Abd El-Rahman, S. S.
AU - Mahmoud, M. Y.
AU - Noshy, P. A.
C6 - APR 2021
DA - MAR
DO - 10.1007/s12011-021-02713-2
IS - 3
PY - 2022
SN - 0163-4984
1559-0720
SP - 1201-1211
ST - Evaluation of the Ameliorative Effect of Zinc Nanoparticles against Silver
Nanoparticle-Induced Toxicity in Liver and Kidney of Rats
TI - Evaluation of the Ameliorative Effect of Zinc Nanoparticles against Silver
Nanoparticle-Induced Toxicity in Liver and Kidney of Rats
VL - 200
ID - 6192
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) have various pharmaceutical and biomedical
applications owing to their unique physicochemical properties. Zinc (Zn) is an
essential trace element, a strong antioxidant, and has a primary role in gene
expression, enzymatic reactions, and protein synthesis. The present study aims to
explore the toxic effects of Ag-NPs (50 nm) on the liver and kidney of rats and
also to evaluate the potential protective effect of Zn-NPs (100 nm) against these
adverse effects. Forty adult Sprague–Dawley rats were randomly divided into four
equal groups: control group, Ag-NPs group, Zn-NPs group, and Ag-NPs + Zn-NPs group.
Ag-NPs (50 mg/kg) and/or Zn-NPs (30 mg/kg) were administered daily by gavage for 90
days. The results showed that exposure to Ag-NPs increased serum ALT, AST, urea,
and creatinine. Ag-NPs also induced oxidative stress and lipid peroxidation and
increased inflammatory cytokines in hepatic and renal tissues. Moreover,
histopathological and immunohistochemical examinations revealed various
histological alterations and positive caspase-3 expressions in the liver and kidney
following exposure to Ag-NPs. On the other hand, most of these toxic effects were
ameliorated by co-administration of Zn-NPs. It was concluded that Ag-NPs have
hepatotoxic and nephrotoxic effects in rats via different mechanisms including
oxidative stress, inflammation, and apoptosis and that Zn-NPs can be used to
alleviate these harmful effects by their antioxidative, anti-inflammatory, and
antiapoptotic properties. © 2021, The Author(s), under exclusive licence to
AU - Shehata, A. M.
AU - Salem, F. M. S.
AU - El-Saied, E. M.
AU - Abd El-Rahman, S. S.
AU - Mahmoud, M. Y.
AU - Noshy, P. A.
DB - Scopus
DO - 10.1007/s12011-021-02713-2
IS - 3
KW - Antioxidants
Caspase-3
Hepatotoxicity
Lipid peroxidation
Nephrotoxicity
Animals
Kidney
Liver
Metal Nanoparticles
Oxidative Stress
Rats
Silver
Zinc
drug
metal nanoparticle
silver
zinc
animal
kidney
liver
metabolism
oxidative stress
rat
Sprague Dawley rat
M3 - Article
PY - 2022
SP - 1201-1211
ST - Evaluation of the Ameliorative Effect of Zinc Nanoparticles against Silver
Nanoparticle–Induced Toxicity in Liver and Kidney of Rats
TI - Evaluation of the Ameliorative Effect of Zinc Nanoparticles against Silver
Nanoparticle–Induced Toxicity in Liver and Kidney of Rats
85104685123&doi=10.1007%2fs12011-021-02713-
2&partnerID=40&md5=372fb4be2a3e20187bc77add34eb5e33
VL - 200
ID - 5058
ER -
TY - JOUR
AB - Wound dressings have become a crucial treatment for wound healing due to
their convenience, low cost, and prolonged wound management. As cutting-edge
biomaterials, marine polysaccharides are divided from most marine organisms. It
possesses various bioactivities, which al-lowing them to be processed into various
forms of wound dressings. Therefore, a comprehensive understanding of the
application of marine polysaccharides in wound dressings is particularly important
for the studies of wound therapy. In this review, we first introduce the wound
healing process and describe the characteristics of modern commonly used dressings.
Then, the properties of various marine polysaccharides and their application in
wound dressing development are outlined. Finally, strategies for developing and
enhancing marine polysaccharide wound dressings are de-scribed, and an outlook of
these dressings is given. The diverse bioactivities of marine polysaccharides
including antibacterial, anti-inflammatory, haemostatic properties, etc., providing
excellent wound management and accelerate wound healing. Meanwhile, these
biomaterials have higher biocompatibility and biodegradability compared to
synthetic ones. On the other hand, marine polysaccharides can be combined with
copolymers and active substances to prepare various forms of dressings. Among them,
emerging types of dressings such as nanofibers, smart hydrogels and injectable
hydrogels are at the research frontier of their development. Therefore, marine
polysaccharides are essential materials in wound dressings fabrication and have a
promising future. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Shen, S.
AU - Chen, X.
AU - Shen, Z.
AU - Chen, H.
C7 - 1666
DB - Scopus
IS - 10
KW - Agar
Alginate
Biomaterials
Biopolymers
Carrageenan
Chitosan
Fu-coidan
Ulvan
Wound dressing
Wound healing
5 hydroxymethylfurfural
agar
agarose
alginic acid
andrographolide
Arnica montana extract
bioceramics
biomaterial
borate sodium
calcium sulfate
Calendula extract
carbon nanotube
carrageenan
chitin
chitosan
chondroitin sulfate
ciprofloxacin
copolymer
curcumin
dermatan sulfate
dopamine
doxorubicin
doxycycline
erythromycin
essential oil
exopolysaccharide
Forsythia extract
fucoidin
gel
genipin
gentamicin
glass
glycosaminoglycan
graphene
graphene oxide
heparan sulfate
heparin
hyaluronic acid
hydrogel
ibuprofen
keratan sulfate
laminaran
lidocaine
macrogol
marine polysaccharide
melatonin
minocycline
mupirocin
nanofiber
naproxen
polycaprolactone
polylactic acid
polysaccharide
Schiff base
silver
silver nanoparticle
stearic acid
streptomycin
tannin
tetracycline
thrombocyte concentrate
tranexamic acid
ulvan
unclassified drug
vancomycin
vasculotropin
zinc ion
zinc nanoparticle
zinc oxide
Aloe vera
biocompatibility
biodegradability
biomechanics
chemical structure
coating (procedure)
drug release
emulsion
gelation
hemostasis
honey
human
nanofabrication
nonhuman
oregano
Review
thermostability
tissue regeneration
wound care
wound healing
M3 - Review
PY - 2021
ST - Marine polysaccharides for wound dressings application: An overview
T2 - Pharmaceutics
TI - Marine polysaccharides for wound dressings application: An overview
85117787885&doi=10.3390%2fpharmaceutics13101666&partnerID=40&md5=104017426870c98535
8d90f8e07b2caa
VL - 13
ID - 5183
ER -
TY - JOUR
AB - Background: Nanopopcorns are a novel class of metallic nanoparticles that
demonstrate structural similarity to the grains of popcorns with theranostic
activities for diseases like cancer and bacterial infection using Surface Enhanced
Raman Spectroscopy-based detection. Objective: The objective of the present article
is to highlight the importance of popcorn-shaped nanoparticles for the treatment of
various disease conditions like cancer, diabetes, ulcerative colitis, rheumatoid
arthritis, etc. Methods: Nanopopcorns enter the target cells via conjugation with
various proteins, aptamers, etc. to kill the diseased cell. Moreover, external
magnetic radiations are provided to heat these metallic nanopopcorns for creating
hotspots. All such activities can be tracked via SERS mechanism. Results:
Nanopopcorns create alternative and minimally-invasive treatment strategies for
inflammatory conditions and life-threatening diseases. Conclusion: In the near
future, nanopopcorn-based drug delivery system can be an interesting field for
research in medicinal nanotechnology. © 2021 Bentham Science Publishers.
AU - Shende, P.
AU - Deshpande, G.
DB - Scopus
DO - 10.2174/1573413716999201209105519
IS - 5
KW - Apoptosis
Cell imaging
Hotspots
Hyperthermia
Surface enhanced raman spectroscopy
Theranostics
Cell death
Drug delivery
Light transmission
Metals
Nanoparticles
Raman spectroscopy
dye
gold nanoparticle
metal nanoparticle
nanomaterial
nanorod
nanosphere
pesticide
protective agent
silver nanoparticle
Metallics
Multi-modal approach
Structural similarity
Surface enhanced Raman spectroscopy
apoptosis
Article
bacterium
chemical interaction
conjugation
diabetes mellitus
electromagnetism
human
magnetic field
malignant neoplasm
particle size
thermogravimetry
ulcerative colitis
zeta potential
Diseases
M3 - Article
PY - 2021
SP - 670-678
ST - Metallic nanopopcorns: A new multimodal approach for theranostics
T2 - Current Nanoscience
TI - Metallic nanopopcorns: A new multimodal approach for theranostics
85119695856&doi=10.2174%2f1573413716999201209105519&partnerID=40&md5=24083bb8b6790f
7a082dabcc40888564
VL - 17
ID - 5169
ER -
TY - JOUR
AB - Epidemiological studies have indicated that particulate matter (PM) exposure
is most likely relevant to atherosclerosis. Endothelial cell injury and dysfunction
are considered the early events in the initiation of atherosclerosis. Silver
nanoparticles (AgNPs), with a smaller size and higher reactive activity, may induce
much higher toxicity to endothelial cells compared with PM. However, few studies
have been performed to determine the effect of AgNPs on endothelial cells. In the
present study, human umbilical vein endothelial cells (HUVECs) were chosen as model
cells to systematically explore the toxicity of AgNPs to endothelial cells. The
obtained results indicated that exposure to AgNPs could inhibit proliferation,
damage the cell membrane and seriously induce apoptosis. Simultaneously, the
inflammatory cytokines, adhesion molecules, and chemokines of HUVECs were clearly
up-regulated, which resulted in the adhesion of many monocytes to endothelial
cells. More importantly, we found that dysfunctions of endothelial cells could be
ascribed to the activation of NF-kappa B pathways. Furthermore, an oxidation
inhibitor, N-acetyl-L-cysteine (NAC), effectively antagonized all of the AgNPs-
induced responses, which indicated the key role of ROS production during the
exposure of AgNPs in the toxicity of endothelial cells. In summary, our results
clearly demonstrated that AgNPs could induce the injury and dysfunction of HUVECs
through the activation of IKK/NF-kappa B, which is associated with oxidative
stress, suggesting that exposure to AgNPs may be a potential hazardous factor for
early atherosclerosis. (C) 2014 Elsevier Ltd. All rights reserved.
AN - WOS:000338804500049
AU - Shi, J. P.
AU - Sun, X.
AU - Lin, Y.
AU - Zou, X. Y.
AU - Li, Z. J.
AU - Liao, Y. Y.
AU - Du, M. M.
AU - Zhang, H. W.
DA - AUG
IS - 24
PY - 2014
SN - 0142-9612
1878-5905
SP - 6657-6666
ST - Endothelial cell injury and dysfunction induced by silver nanoparticles
through oxidative stress via IKK/NF-kappa B pathways
T2 - BIOMATERIALS
TI - Endothelial cell injury and dysfunction induced by silver nanoparticles
through oxidative stress via IKK/NF-kappa B pathways
VL - 35
ID - 5959
ER -
TY - JOUR
AB - Epidemiological studies have indicated that particulate matter (PM) exposure
is most likely relevant to atherosclerosis. Endothelial cell injury and dysfunction
are considered the early events in the initiation of atherosclerosis. Silver
nanoparticles (AgNPs), with a smaller size and higher reactive activity, may induce
much higher toxicity to endothelial cells compared with PM. However, few studies
have been performed to determine the effect of AgNPs on endothelial cells. In the
present study, human umbilical vein endothelial cells (HUVECs) were chosen as model
cells to systematically explore the toxicity of AgNPs to endothelial cells. The
obtained results indicated that exposure to AgNPs could inhibit proliferation,
damage the cell membrane and seriously induce apoptosis. Simultaneously, the
inflammatory cytokines, adhesion molecules, and chemokines of HUVECs were clearly
up-regulated, which resulted in the adhesion of many monocytes to endothelial
cells. More importantly, we found that dysfunctions of endothelial cells could be
ascribed to the activation of NF-κB pathways. Furthermore, an oxidation inhibitor,
N-acetyl- l-cysteine (NAC), effectively antagonized all of the AgNPs-induced
responses, which indicated the key role of ROS production during the exposure of
AgNPs in the toxicity of endothelial cells. In summary, our results clearly
demonstrated that AgNPs could induce the injury and dysfunction of HUVECs through
the activation of IKK/NF-κB, which is associated with oxidative stress, suggesting
that exposure to AgNPs may be a potential hazardous factor for early
atherosclerosis. © 2014 Elsevier Ltd.
AU - Shi, J.
AU - Sun, X.
AU - Lin, Y.
AU - Zou, X.
AU - Li, Z.
AU - Liao, Y.
AU - Du, M.
AU - Zhang, H.
DB - Scopus
IS - 24
Endothelium
NF-κB
Apoptosis
Cell Adhesion
Cell Adhesion Molecules
Cell Death
Cell Line, Tumor
Chemokine CCL2
Cytokines
Endocytosis
Human Umbilical Vein Endothelial Cells
Humans
I-kappa B Kinase
Metal Nanoparticles
Monocytes
NF-kappa B
Oxidative Stress
Phosphorylation
Signal Transduction
Silver
Acetylcysteine
Adhesion
Amino acids
Cell death
Cell membranes
Chemical activation
Diseases
Nanoparticles
Toxicity
autacoid
CCL2 protein, human
cell adhesion molecule
cytokine
I kappa B kinase
metal nanoparticle
silver
Epidemiological studies
apoptosis
cell adhesion
cell death
cytology
drug effects
endocytosis
human
metabolism
monocyte
oxidative stress
pathology
phosphorylation
signal transduction
toxicity
tumor cell line
ultrastructure
Endothelial cells
M3 - Article
PY - 2014
SP - 6657-6666
ST - Endothelial cell injury and dysfunction induced by silver nanoparticles
through oxidative stress via IKK/NF-κB pathways
T2 - Biomaterials
TI - Endothelial cell injury and dysfunction induced by silver nanoparticles
through oxidative stress via IKK/NF-κB pathways
84901394106&doi=10.1016%2fj.biomaterials.2014.04.093&partnerID=40&md5=c72d24181e640
006e67e46fe65b62f0b
VL - 35
ID - 5575
ER -
TY - JOUR
AB - Fracture-related infections require both treatments for bacteria removal and
bone reconstruction. The use of combined broadspectrum antibacterial silver
compounds and artificial bone with high osteogenic activity is considered to be an
effective strategy for achieving these treatments in one surgery. However, silver
compounds are toxic for living tissues even at low concentrations. Herein, we
investigated the no-observed-effect level (NOEL) of silver phosphate (Ag3PO4) in a
bone substitute composed of carbonate apatite (CO(3)Ap), a bone mineral, using in
vitro and in vivo experiments. In vitro experiments demonstrated that the CO(3)Ap
artificial bone containing >= 0.1 wt % Ag3PO4 exerted antibacterial effects against
Staphylococcus epidermidis, while those containing wt % Ag3PO4 did not affect
cellular adhesion, proliferation, differentiation, and calcification of osteoblast-
like MC3T3-E1 cells. In vivo experiments demonstrated that the CO(3)Ap artificial
bone containing wt % Ag3PO4 replaced a new bone to the same levels as those without
Ag3PO4 4 weeks after implantation into the bone defect of the rabbit femur condyle.
However, the CO(3)Ap artificial bone containing <= 0.3 wt % Ag3PO4 caused an
inflammatory reaction, whereas those containing wt <= 0.1% Ag3PO4 did not. Thus,
both bone regeneration and infection control without any adverse effects were
achieved using the CO(3)Ap artificial bone containing 0.1 wt % Ag3PO4, indicating
that the NOEL of Ag3PO4 was 0.1 wt %. Our results provide an effective strategy for
the treatments of fracture-related infections.
AN - WOS:000730568800001
AU - Shimabukuro, M.
AU - Hayashi, K.
AU - Kishida, R.
AU - Tsuchiya, A.
AU - Ishikawa, K.
C6 - DEC 2021
DA - JAN 14
DO - 10.1021/acsinfecdis.1c00480
IS - 1
PY - 2022
SN - 2373-8227
SP - 159-169
ST - No-Observed-Effect Level of Silver Phosphate in Carbonate Apatite Artificial
Bone on Initial Bone Regeneration
T2 - ACS INFECTIOUS DISEASES
TI - No-Observed-Effect Level of Silver Phosphate in Carbonate Apatite Artificial
Bone on Initial Bone Regeneration
VL - 8
ID - 6264
ER -
TY - JOUR
AB - Nano-silver is used for its anti-bacterial, anti-viral and anti-fungal
properties. The aim of this study was to investigate the potential clinical
efficacy of nano-silver for its anti-inflammatory effect on respiratory epithelial
cell inflammation. Primary nasal polyp epithelial cells (NPECs) were exposed to
Alternaria alternata, Der P1, and staphylococcal enterotoxin B for 48 h with or
without various concentration of nano-silver, then the supernatants were collected.
Cell cytotoxicities were measured using a CellTiter-96® aqueous cell proliferation
assay kit. The interleukin (IL)-6, IL-8 and granulocyte-macrophage colony
stimulating factor were measured to evaluate the inflammatory effects on the
epithelial cells. Nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) were
analyzed using western blot and ELISA method. Cell survival was found to be
significantly decreased at nano-silver concentrations exceeding 10 ppm. Alternaria,
Der P1 and SEB activated NPECs with increased cytokine production. Alternaria
induced NPECs not inhibited by nano-silver. However, Der P1 and SEB induced
cytokine production was significantly affected by concentrations over 1 ppm.
Alternaria, Der P1 and SEB enhanced nuclear NF-κB expression and nano-silver
inhibited NF-κB expression in SEB and Der P1 treated group. Although nano-silver is
cytotoxic at higher concentrations, at safe concentrations it can inhibit the
activation of NPECs. This finding suggests a novel pharmacological rationale for
the treatment of airway inflammation and/or immunological disease. © 2011 Elsevier
B.V. All rights reserved.
AU - Shin, S. H.
AU - Ye, M. K.
DB - Scopus
DO - 10.1016/j.intimp.2011.05.028
IS - 11
KW - Alternaria
DerP1
Epithelial cell
Nano-silver
NF-κB
Staphylococcal enterotoxin B
Antigens, Dermatophagoides
Arthropod Proteins
Blotting, Western
Cell Proliferation
Cell Survival
Cells, Cultured
Colloids
Cysteine Endopeptidases
Cytokines
Enterotoxins
Epithelial Cells
Humans
Nanoparticles
Nasal Polyps
Particle Size
Silver Compounds
house dust allergen
interleukin 6
interleukin 8
silver nanoparticle
Staphylococcus enterotoxin B
Alternaria alternata
article
cell proliferation
cell survival
clinical article
cytokine production
cytotoxicity
epithelium cell
human
human cell
nose polyp
priority journal
Western blotting
M3 - Article
PY - 2011
SP - 1691-1696
ST - The effect of nano-silver on the activation of nasal polyp epithelial cells
by Alternaria, der P1 and staphylococcal enterotoxin B
TI - The effect of nano-silver on the activation of nasal polyp epithelial cells
by Alternaria, der P1 and staphylococcal enterotoxin B
80055022638&doi=10.1016%2fj.intimp.2011.05.028&partnerID=40&md5=d250566169b933a47a0
b189e75555965
VL - 11
ID - 5668
ER -
TY - JOUR
AB - Objectives. Silver has long been known as a strong antimicrobial and
disinfectant. Several types of nano-silver coated products have been developed.
However, the antimicrobial and disinfectant characteristics of nano-silver have not
been well studied. The aim of this study was to investigate the effect of nano-
silver on allergic inflammation in a mouse model. Methods. Female BALB/C mice were
sensitized by intraperitoneal injection of ovalbumin (OVA) and aluminium hydroxide
on days 0, 7, 14, and 21. Mice were challenged with intranasal instillation of OVA.
Nano-silver was also administered nasally prior to intranasal instillation of OVA.
Severity of allergic rhinitis was assessed according to nasal symptoms, serum OVA-
specific IgE level, interleukin (IL)-4, IL-10, and interferon (INF)-gamma levels in
nasal lavage fluid. Hematoxylin-eosin stain and periodic acid-Schiff stain were
performed for evaluation of histological change. Results. Nano-silver attenuated
manifestation of nasal symptoms in sensitized mice and inhibited production of OVA-
specific IgE, IL-4, and IL-10, however, it had no effect on INF-gamma level. In
addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia
was attenuated by nano-silver. Conclusion. These results suggest that nano-silver
may effectively reduce allergic inflammation in a mouse model of allergic
rhinitis.Through its properties as an anti-inflammatory agent, nano-silver may be a
useful therapeutic strategy.
AN - WOS:000312046900008
AU - Shin, S. H.
AU - Ye, M. K.
DA - DEC
DO - 10.3342/ceo.2012.5.4.222
IS - 4
PY - 2012
SN - 1976-8710
SP - 222-227
ST - The Effect of Nano-Silver on Allergic Rhinitis Model in Mice
T2 - CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY
TI - The Effect of Nano-Silver on Allergic Rhinitis Model in Mice
VL - 5
ID - 6358
ER -
TY - JOUR
AB - Silver could prove to be a valuable alternative raw material for antibiotics
and disinfectants as it is relatively free of adverse effects. Nano-silver is now
been put to practical use in commonly used items, such as, clothes, electric home
appliances, and electronic industry, but has not been widely applied in the medical
or pharmacological fields. This study was designed to investigate the effects of
nano-silver on the production of cytokines by and on the proliferation of
peripheral blood mononuclear cells (PBMCs). In addition, we investigated the
potential cytotoxic effects of nano-silver on PBMCs. PBMCs from healthy human
volunteers were stimulated with 5 μg/ml phytohaemagglutinin (PHA) in the presence
of varying concentrations of nano-silver. PBMC proliferations were measured using
an aqueous cell proliferation assay kit and supernatants were analyzed using
enzyme-linked immunosorbent assays. Interleukin-5 (IL-5), interferon-γ (INF-γ), and
tumor necrosis factor-α (TNF-α) protein levels were measured to determine the
activation state of PBMCs. At levels of over 15 ppm, nano-silver was found to have
a significant cytotoxic effect on PBMCs, and PHA-induced cytokine productions were
significantly inhibited by nano-silver (IL-5: at 10 ppm, INF-γ and TNF-α at 3 ppm).
Although nano-silver had a cytotoxic effect at high concentration, nano-silver
modulated cytokine production in a concentration-dependent manner. These
experimental data suggest that nano-silver could be used to treat immunologic and
inflammatory diseases. © 2007 Elsevier B.V. All rights reserved.
AU - Shin, S. H.
AU - Ye, M. K.
AU - Kim, H. S.
AU - Kang, H. S.
DB - Scopus
DO - 10.1016/j.intimp.2007.08.025
IS - 13
KW - Activation
Cytokine
Nano-silver
Peripheral blood mononuclear cell
Proliferation
Cell Proliferation
Cytokines
Humans
Interferon Type II
Interleukin-5
Nanoparticles
Silver
cytokine
gamma interferon
interleukin 5
medisil
nanoparticle
nanosilver
phytohemagglutinin
unclassified drug
article
cell activation
cell proliferation
controlled study
cytokine production
cytotoxicity
human
human cell
immunopathology
priority journal
protein expression
M3 - Article
PY - 2007
SP - 1813-1818
ST - The effects of nano-silver on the proliferation and cytokine expression by
TI - The effects of nano-silver on the proliferation and cytokine expression by
35748933519&doi=10.1016%2fj.intimp.2007.08.025&partnerID=40&md5=34772f30f42143a283c
154e509c884a2
VL - 7
ID - 5808
ER -
TY - JOUR
AB - The most frequent complication in external fixation is pin tract infection.
To reduce the incidence of implant-associated infection, many published reports
have looked at preventing bacterial adhesion by treating the pin surface. This
study aimed to evaluate the antibacterial activity of a Titanium-Copper (Ti-Cu)
alloy on implant infection, and to determine the potential use of the Ti-Cu alloy
as a biomaterial. Two forms of Ti-Cu alloys were synthesized: one with 1% Cu and
the other with 5% Cu. For analyzing infectious behavior, the implants were exposed
to Staphylococcus aureus and Escherichia coli. The reaction of pathogens to the Ti-
Cu alloys was compared with their reaction to stainless steel and pure titanium as
controls. Both Ti-Cu alloys evidently inhibited colonization by both bacteria.
Conversely, cytocompatibility studies were performed using fibroblasts and colony
formation on the metals was assessed by counting the number of colonies. Ti-1% Cu
alloy showed no difference in the number of colonies compared with the control.
External fixator pins made of Ti-Cu alloys were evaluated in a rabbit model. The
tissue-implant interactions were analyzed for the presence of infection,
inflammatory changes and osteoid-formation. Ti-1% Cu alloy significantly inhibited
inflammation and infection, and had excellent osteoid-formation. Copper blood
levels were measured before surgery and at 14 days postoperatively. Preoperative
and postoperative blood copper values were not statistically different. Overall, it
was concluded that Ti-Cu alloys have antimicrobial activity and substantially
reduce the incidence of pin tract infection. Ti-1% Cu alloy shows particular
promise as a biomaterial. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part
B: Appl Biomater 91B: 373-380, 2009
AN - WOS:000269897100043
AU - Shirai, T.
AU - Tsuchiya, H.
AU - Shimizu, T.
AU - Ohtani, K.
AU - Zen, Y.
AU - Tomita, K.
DA - OCT
DO - 10.1002/jbm.b.31412
IS - 1
PY - 2009
SN - 1552-4973
1552-4981
SP - 373-380
ST - Prevention of Pin Tract Infection with Titanium-Copper Alloys
TI - Prevention of Pin Tract Infection with Titanium-Copper Alloys
VL - 91B
ID - 6616
ER -
TY - JOUR
AB - Silver (Ag) and gold nanoparticles (Au NPs) have wide applications. They are
increasingly being used in the medical devices, biosensors, cancer cell imaging,
and cosmetics. Increased applications of these NPs in the technological advances
have also led to the risk of exposure to these particles. This study investigated
the toxic effects of Ag and Au NPs (1 μM and 2 μM, oral) on mouse erythrocytes and
tissues after 14 consecutive days' exposure. Our results demonstrate significant
increase in reactive oxygen species (ROS) and depletion of antioxidant enzyme
status in erythrocytes and tissues. Hepatic and renal toxicity was evident from
liver and kidney function tests. Inflammatory markers, interleukin-6 and nitric
oxide synthase increased in plasma on administration following exposure to these
NPs at both the doses. A more pronounced increase was noted in kidney
metallothionein (MT) compared to liver MT on exposure to these NPs. Toxic potential
of these NPs was further confirmed by increased 8-hydroxy-2′-deoxyguanosine levels
in urine, a biomarker of DNA damage. Among the two NPs, Ag NP was more toxic at 2
μM dose compared to lower dose of 1 μM. The study suggests oxidative stress as the
major mechanism responsible for the toxic manifestations induced by Ag and Au NPs.
© The Author(s) 2014.
AU - Shrivastava, R.
AU - Kushwaha, P.
AU - Bhutia, Y. C.
AU - Flora, S. J. S.
DB - Scopus
DO - 10.1177/0748233714562623
IS - 8
KW - dose dependent
gold
in vivo
mice
nanoparticles
oxidative stress
Silver
Animals
Biomarkers
DNA Damage
Gold
Kidney
Liver
Male
Metal Nanoparticles
Mice
Mutagens
Oxidative Stress
Renal Insufficiency
Toxicity Tests, Subacute
cadmium chloride
creatinine
gold nanoparticle
interleukin 6
metallothionein
silver nanoparticle
urea
autacoid
biological marker
metal nanoparticle
mutagenic agent
silver
animal cell
animal experiment
animal model
animal tissue
Article
controlled study
DNA damage
enzyme activity
kidney function test
liver toxicity
male
mouse
nephrotoxicity
nonhuman
protein blood level
weight change
administration and dosage
animal
blood
chemically induced
comparative study
dose response
drug effect
immunology
kidney
kidney failure
liver
metabolism
pathophysiology
pollutant
toxic hepatitis
toxicity
toxicity testing
urine
M3 - Article
PY - 2016
SP - 1391-1404
ST - Oxidative stress following exposure to silver and gold nanoparticles in mice
TI - Oxidative stress following exposure to silver and gold nanoparticles in mice
84980347930&doi=10.1177%2f0748233714562623&partnerID=40&md5=56eb4bf95b38dc4ae1c5873
1a211607a
VL - 32
ID - 5475
ER -
TY - JOUR
AB - Mercury is one of the most toxic elements and causes a multitude of health
problems. It is ten times more toxic to neurons than lead. This study was created
to determine if mercury could be causing Alzheimer's disease (AD) by cross
referencing the effects of mercury with 70 factors associated with AD. The results
found that all these factors could be attributed to mercury. The hallmark changes
in AD include plaques, beta amyloid protein, neurofibrillary tangles,
phosphorylated tau protein, and memory loss-all changes that can be caused by
mercury. Neurotransmitters such as acetylcholine, serotonin, dopamine, glutamate,
and norepinephrine are inhibited in patients with Alzheimer's disease, with the
same inhibition occurring in mercury toxicity. Enzyme dysfunction in patients with
Alzheimer's disease include BACE 1, gamma secretase, cyclooxygenase-2, cytochrome-
c-oxidase, protein kinases, monoamine oxidase, nitric oxide synthetase, acetyl
choline transferase, and caspases, all which can be explained by mercury toxicity.
Immune and inflammatory responses seen in patients with Alzheimer's disease also
occur when cells are exposed to mercury, including complement activation, cytokine
expression, production of glial fibrillary acid protein antibodies and interleukin-
1, transforming growth factor, beta 2 microglobulins, and phosphodiesterase 4
stimulation. Genetic factors in patients with Alzheimer's disease are also
associated with mercury. Apolipoprotein E 4 allele increases the toxicity of
mercury. Mercury can inhibit DNA synthesis in the hippocampus, and has been
associated with genetic mutations of presenilin 1 and 2, found in AD. The
abnormalities of minerals and vitamins, specifically aluminum, calcium, copper,
iron, magnesium, selenium, zinc, and vitamins B1, B12, E, and C, that occur in
patients with Alzheimer's disease, also occur in mercury toxicity. Aluminum has
been found to increase mercury's toxicity. Likewise, similar biochemical factors in
AD are affected by mercury, including changes in blood levels of homocysteine,
arachidonic acid, DHEA sulfate, glutathione, hydrogen peroxide, glycosamine
glycans, acetyl-L carnitine, melatonin, and HDL. Other factors seen in Alzheimer's
disease, such as increased platelet activation, poor odor identification,
hypertension, depression, increased incidences of herpes virus and chlamydia
infections, also occur in mercury exposure. In addition, patients diagnosed with
Alzheimer's disease exhibit higher levels of brain mercury, blood mercury, and
tissue mercury in some studies. The greatest exogenous sources of brain mercury
come from dental amalgams. Conclusion: This review of the literature strongly
suggests that mercury can be a cause of Alzheimer's Disease.
AN - WOS:000507312700266
AU - Siblerud, R.
AU - Mutter, J.
AU - Moore, E.
AU - Naumann, J.
AU - Walach, H.
C7 - 5152
DA - DEC 2
DO - 10.3390/ijerph16245152
IS - 24
PY - 2019
SN - 1661-7827
1660-4601
ST - A Hypothesis and Evidence That Mercury May be an Etiological Factor in
Alzheimer's Disease
TI - A Hypothesis and Evidence That Mercury May be an Etiological Factor in
Alzheimer's Disease
VL - 16
ID - 6786
ER -
TY - JOUR
AB - Context: Recent findings indicating the anti-inflammatory action of silver
preparations through modulation of the gut microbiota and apoptosis of inflammatory
cells predestine silver use in inflammatory bowel disease (IBD). Objective: The aim
of our study was to validate the possibility of effective silver release from
silver-coated glass beads for anti-inflammatory local application in the lower
sections of the gastrointestinal (GI) tract. Materials and methods: Silver-coated
glass beads were prepared using magnetron method. Release of silver from the
silver-coated glass bead surface was carried out in BIO-DIS reciprocating cylinder
apparatus. Erosion of silver coating and indirect estimation of the silver release
dynamics was assessed using scanning electron microscope. Rectal suppositories
containing silver-coated glass beads were prepared using five different methods
(M1–M5) and X-ray scanned for their composition. Results and discussion: The XR
microanalysis and the chemical composition analysis evidenced for a rapid (within
30 min) release of nearly 50% of silver from the coating of the glass beads, which
remained stable up to 24 h of incubation. The most homogeneous distribution of
beads in the entire volume of the suppository was obtained for formulation M5,
where the molten base was poured into mold placed in an ice bath, and the beads
were added after 10 s. Conclusions: Our study is the first to present the concept
of enclosing silver-coated glass beads in the lipophilic suppository base to
attenuate inflammation in the lower GI tract and promises efficient treatment with
reduced side effects. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
AU - Siczek, K.
AU - Fichna, J.
AU - Zatorski, H.
AU - Karolewicz, B.
AU - Klimek, L.
AU - Owczarek, A.
DB - Scopus
DO - 10.1080/10837450.2017.1359843
IS - 3
KW - drug delivery
metallic silver layer
Silver-coated glass beads
suppository
Administration, Rectal
Dosage Forms
Excipients
Gastrointestinal Tract
Glass
Inflammation
Silver
drug carrier
silver
silver coated glass bead
suppository base
Theobroma oil
unclassified drug
excipient
glass
Article
drug coating
drug formulation
drug mechanism
drug release
human
in vitro study
lower gastrointestinal tract
priority journal
surface property
X ray microanalysis
x-ray computed tomography
chemistry
diagnostic imaging
drug dosage form
inflammation
procedures
rectal drug administration
M3 - Article
PY - 2018
SP - 295-300
ST - Development of the rectal dosage form with silver-coated glass beads for
local-action applications in lower sections of the gastrointestinal tract
T2 - Pharmaceutical Development and Technology
TI - Development of the rectal dosage form with silver-coated glass beads for
local-action applications in lower sections of the gastrointestinal tract
85026870821&doi=10.1080%2f10837450.2017.1359843&partnerID=40&md5=5dbc08444b57b7fcc9
f54ae74c4da290
VL - 23
ID - 5535
ER -
TY - JOUR
AB - Background Recent studies point at the anti-inflammatory action of silver
through induction of apoptosis of inflammatory cells via oxidative stress,
promotion of wound healing as well as antimicrobial effect. Our aim was to design a
new formulation based on silver and validate its anti-inflammatory activity in the
mouse models of colitis. Methods Silver-coated glass beads were prepared using a
magnetron sputtering method and a standard magnetron sputtering gun equipped with
pure silver target. Colitis was induced by the ic administration of TNBS into colon
(to mimic Crohn's disease) and addition of DSS to drinking water (to imitate
ulcerative colitis). Evaluation of inflammation was performed based on macroscopic
and microscopic scoring, quantification of the myeloperoxidase activity and colonic
microflora analysis. Results Silver-coated glass beads administered ic alleviated
intestinal inflammation in mouse models of colitis, induced by TNBS and DSS. This
alleviation of colitis resulted principally from changes in the gut microflora. The
anti-inflammatory action of the new formulation was associated predominantly with
the presence of the silver nanolayer on the beads, and to a lesser extent the size
of glass polymer units. Conclusions The application of the newly developed
formulation employing silver-coated glass beads has the potential to be translated
to clinical conditions for the efficient treatment of IBD. © 2017 Institute of
Pharmacology, Polish Academy of Sciences
AU - Siczek, K.
AU - Zatorski, H.
AU - Chmielowiec-Korzeniowska, A.
AU - Kordek, R.
AU - Tymczyna, L.
AU - Fichna, J.
DB - Scopus
DO - 10.1016/j.pharep.2017.01.003
IS - 3
KW - Crohn's disease
Microbiota
Silver-coated glass beads
Ulcerative colitis
Animals
Chemistry, Pharmaceutical
Colitis
Colitis, Ulcerative
Crohn Disease
Dextran Sulfate
Inflammation
Male
Mice
Mice, Inbred BALB C
Silver
Trinitrobenzenesulfonic Acid
myeloperoxidase
silver
silver coated glass bead
unclassified drug
dextran sulfate
trinitrobenzenesulfonic acid
animal experiment
animal model
animal tissue
Article
colon flora
controlled study
drug effect
drug formulation
drug screening
enzyme activity
experimental colitis
intracolonic drug administration
male
mouse
nonhuman
animal
Bagg albino mouse
chemistry
colitis
Crohn disease
disease model
inflammation
medicinal chemistry
pathology
procedures
M3 - Article
PY - 2017
SP - 386-392
ST - Evaluation of anti-inflammatory effect of silver-coated glass beads in mice
with experimentally induced colitis as a new type of treatment in inflammatory
bowel disease
T2 - Pharmacological Reports
TI - Evaluation of anti-inflammatory effect of silver-coated glass beads in mice
with experimentally induced colitis as a new type of treatment in inflammatory
bowel disease
85014256832&doi=10.1016%2fj.pharep.2017.01.003&partnerID=40&md5=99bc4a9ed5a8eef3d70
55c8bf3045bf8
VL - 69
ID - 5466
ER -
TY - JOUR
AB - Toxic epidermal necrolysis (TEN) is a rare severe reaction of the skin
resulting in full thickness damage to the epidermis. The condition has significant
morbidity as a result of dehydration, protein loss, thermoregulatory difficulties,
and renal, lung, liver and heart failure. The mortality rate approaches 30%, most
commonly from bacterial sepsis. Management of this condition is cessation of the
suspected causative agent and supportive care on a burns or intensive care unit.
There have been recent reports of treatment using intravenous immunoglobulin (IVIG)
therapy, though its efficacy is yet to be established. It has been proposed that
IVIG inhibits the Fas-FasL mediated apoptosis of keratinocytes affected by TEN. We
describe a case of extensive drug-induced TEN in a 33-year-old woman who showed
rapid improvement with IVIG therapy at a dose of 0.75 g/kg/day given for four
consecutive days.
AU - Sidwell, R. U.
AU - Swift, S.
AU - Yan, C. L.
AU - Porter, W.
AU - Thompson, E. M.
AU - Clark, J. A.
AU - Bunker, C. B.
DB - Scopus
IS - 7
KW - Adult
Antidepressive Agents, Second-Generation
Antimanic Agents
Carbamazepine
Cyclohexanols
Depression, Postpartum
Epidermal Necrolysis, Toxic
Female
Humans
Immunoglobulins, Intravenous
amphotericin
beclometasone dipropionate
betamethasone valerate
carbamazepine
clobetasol propionate
dexamethasone
diflucortolone valerate
flucloxacillin
immunoglobulin
myristic acid isopropyl ester
paraffin
penicillin G
sulfadiazine silver
venlafaxine
adult
article
blister
case report
cell death
chemosis
conjunctivitis
cornea erosion
drug withdrawal
female
general condition deterioration
genital ulcer
human
human cell
human tissue
keratinocyte
mouth ulcer
neutrophilia
priority journal
puerperal depression
rash
skin biopsy
skin defect
skin exfoliation
toxic epidermal necrolysis
treatment outcome
M3 - Article
PY - 2003
SP - 643-645
ST - Treatment of toxic epidermal necrolysis with intravenous immunoglobulin
T2 - International Journal of Clinical Practice
TI - Treatment of toxic epidermal necrolysis with intravenous immunoglobulin
0141502380&partnerID=40&md5=151877d4c5b31131e6feaf9c9618ce46
VL - 57
ID - 5829
ER -
TY - JOUR
AB - Chronic Hepatitis C Virus (HCV) infection is associated with progressive
liver injury and subsequent development of fibrosis and cirrhosis. The death of
hepatocytes results in the release of cytokines that induce inflammatory and
fibrotic responses. The mechanism of liver damage is still under investigation but
both apoptosis and immune-mediated processes may play roles. By observing the
changes in gene expression patterns in HCV-infected cells, both markers and the
causes of HCV-associated liver injury may be elucidated. HCV genotype 1b virus from
persistently infected VeroE6 cells induced a strong cytopathic effect when used to
infect Huh7.5 hepatoma cells. To determine if this cytopathic effect was a result
of apoptosis, ultrastructural changes were observed by electron microscopy and
markers of programmed cell death were surveyed. Screening of a human PCR array
demonstrated a gene expression profile that contained upregulated markers of
apoptosis, including tumor necrosis factor, caspases and caspase activators, Fas,
Bcl2-interacting killer (BIK) and tumor suppressor protein, p53, as a result of HCV
genotype 1b infection. The genes identified in this study should provide new
insights into understanding viral pathogenesis in liver cells and may possibly help
to identify novel antiviral and antifibrotic targets.
AN - WOS:000377563000005
AU - Silberstein, E.
AU - Ulitzky, L.
AU - Lima, L. A.
AU - Cehan, N.
AU - Teixeira-Carvalho, A.
AU - Roingeard, P.
AU - Taylor, D. R.
C7 - e0155708
DA - JUN 9
DO - 10.1371/journal.pone.0155708
IS - 6
PY - 2016
SN - 1932-6203
ST - HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis
T2 - PLOS ONE
TI - HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis
VL - 11
ID - 6497
ER -
TY - JOUR
AB - Titanium dioxide nanoparticles (TiO2 NP) are present in several daily use
products, and the risks associated with their bioaccumulation must be stablished.
Thus, an evaluation of several toxicological-related effects was conducted after
intraperitoneal injection of TiO2 NPs in mice. Mice were divided into two groups,
which received 2 mg kg(-1) day(-1) of TiO2 NPs or vehicle saline. Assessments of
body and organ weight as well as biochemical, hematological, and histopathological
analyses were performed in order to evaluate adverse effects. The results showed
that treatment resulted in an increased visceral and abdominal fat deposition, as
well as a mononuclear inflammatory infiltrates in the abdominal fat tissue. The
TiO2 NPs induced significant decrease in the weight gain and splenomegaly.
Additionally, TiO2 NP-treated mice showed altered hematological parameters and
significant liver injuries, which were characterized by histopathological and
biochemical changes. Our results also indicated that TiO2 NPs were absorbed and
significantly accumulated in the spleen, liver, and kidney. These results showed
the ability of TiO2 NPs to infiltrate different organs and to induce inflammation
and liver and spleen damage with visceral fat accumulation. The data obtained are
useful for the governmental authorities to legislate and implement regulations
concerning the use and the production of this kind of material that might be
hazardous to the living beings, as well as to the environment.
AN - WOS:000397442200005
AU - Silva, A. H.
AU - Locatelli, C.
AU - Filho, U. P. R.
AU - Gomes, B. F.
AU - de Carvalho, R. M.
AU - de Gois, J. S.
AU - Borges, D. L. G.
AU - Creczynski-Pasa, T. B.
DA - FEB
DO - 10.1177/0748233715613224
IS - 2
PY - 2017
SN - 0748-2337
1477-0393
SP - 147-158
ST - Visceral fat increase and signals of inflammation in adipose tissue after
administration of titanium dioxide nanoparticles in mice
TI - Visceral fat increase and signals of inflammation in adipose tissue after
administration of titanium dioxide nanoparticles in mice
VL - 33
ID - 6507
ER -
TY - JOUR
AB - Naturally-occurring chalcones and synthetic chalcone analogues have been
demonstrated to have many biological effects, including anti-inflammatory, anti-
malarial, anti-fungal, and anti-oxidant/anti-cancerous activities. Compared to
other chalcones, trans-chalcone exhibits superior inhibitory activity in cancer
cell growth as shown via in vitro assays, and exerts anti-cancerous effects via the
activation of the p53 tumor suppressor protein. Thus, characterization of the
specific mechanisms, by which trans-chalcone activates p53, can aid development of
new chemotherapeutic drugs that can be used individually or synergistically with
other drugs. In this report, we found that trans-chalcone modulates many p53 target
genes, HSP40 being the most induced gene in the RNA-Seq data using trans-chalcone-
treated cells. CRM1 is also inhibited by trans-chalcone, resulting in the
accumulation of p53 and other tumor suppressor proteins in the nucleus. Similar
effects were seen using trans-chalcone derivatives. Overall, trans-chalcone could
provide a strong foundation for the development of chalcone-based anti-cancer
drugs.
AN - WOS:000442282700020
AU - Silva, G.
AU - Marins, M.
AU - Chaichanasak, N.
AU - Yoon, Y.
AU - Fachin, A. L.
AU - Pinhanelli, V. C.
AU - Regasini, L. O.
AU - dos Santos, M. B.
AU - Ayusso, G. M.
AU - Marques, B. D.
AU - Wu, W. W.
AU - Phue, J. N.
AU - Shen, R. F.
AU - Baek, S. J.
C7 - e0202263
DA - AUG 17
DO - 10.1371/journal.pone.0202263
IS - 8
PY - 2018
SN - 1932-6203
ST - Trans-chalcone increases p53 activity via DNAJB1/HSP40 induction and CRM1
inhibition
T2 - PLOS ONE
TI - Trans-chalcone increases p53 activity via DNAJB1/HSP40 induction and CRM1
inhibition
VL - 13
ID - 6726
ER -
TY - JOUR
AB - Zinc oxide NPs (ZnO) have been recently proposed as novel candidates for the
treatment of allergic inflammatory diseases. Paradoxically, recent data suggested
that ZnO could cause eosinophilic airway inflammation in rodents. Despite the above
observations, there are currently no studies reporting direct interaction between a
given NP and human eosinophils themselves. In this study, freshly isolated human
eosinophils were incubated with ZnO and several cellular functions were studied. We
found that ZnO delay human eosinophil apoptosis, partially by inhibiting caspases
and by preventing caspase-4 and Bcl-xL degradation. ZnO do not induce production of
reactive oxygen species but increase de novo protein synthesis. In addition, ZnO
were found to increase the production of the proinflammatory IL-1 beta and IL-8
cytokines. Using a pharmacological approach, we demonstrated that inhibition of
caspase-1 reversed the ability of ZnO to induce IL-1 beta and IL-8 production,
whereas inhibition of caspase-4 only reversed that of IL-8. Our results indicate
the necessity of conducting studies to determine the potential fusing NP as
nanotherapies, particularly in diseases in which eosinophils may be involved. We
conclude that, indeed, human eosinophils represent potential new direct targets to
NPs, ZnO in the present case. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
AN - WOS:000385332000002
AU - Silva, L. R.
AU - Girard, D.
DA - SEP 30
DO - 10.1016/j.toxlet.2016.07.020
PY - 2016
SN - 0378-4274
1879-3169
SP - 11-20
ST - Human eosinophils are direct targets to nanoparticles: Zinc oxide
nanoparticles (ZnO) delay apoptosis and increase the production of the pro-
inflammatory cytokines IL-1 beta and IL-8
TI - Human eosinophils are direct targets to nanoparticles: Zinc oxide
nanoparticles (ZnO) delay apoptosis and increase the production of the pro-
inflammatory cytokines IL-1 beta and IL-8
VL - 259
ID - 6466
ER -
TY - JOUR
AB - Silver nanoparticle (Ag NP) production methods are being developed and
refined to produce more uniform Ag NPs through chemical reactions involving silver
salt solutions, solvents, and capping agents to control particle formation. These
chemical reactants are often present as contaminants and/or coatings on the Ag NPs,
which could alter their interactions in vivo. To determine pulmonary effects of
citrate-coated Ag NPs, Sprague-Dawley rats were exposed once nose-only to
aerosolized Ag NPs (20 nm [C20] or 110 nm [C110] Ag NPs) for 6 hr. Bronchoalveolar
lavage fluid (BALF) and lung tissues were obtained at 1, 7, 21, and 56 days
postexposure for analyses. Inhalation of Ag NPs, versus citrate buffer control,
produced significant inflammatory and cytotoxic responses that were measured in
BALF cells and supernatant. At day 7, total cells, protein, and lactate
dehydrogenase were significantly elevated in BALF, and peak histopathology was
noted after C20 or C110 exposure versus control. At day 21, BALF polymorphonuclear
cells and tissue inflammation were significantly greater after C20 versus C110
exposure. By day 56, inflammation was resolved in Ag NP-exposed animals. Overall,
results suggest delayed, short-lived inflammatory and cytotoxic effects following
C20 or C110 inhalation and potential for greater responses following C20 exposure.
AN - WOS:000379174900006
AU - Silva, R. M.
AU - Anderson, D. S.
AU - Peake, J.
AU - Edwards, P. C.
AU - Patchin, E. S.
AU - Guo, T.
AU - Gordon, T.
AU - Chen, L. C.
AU - Sun, X. L.
AU - Van Winkle, L. S.
AU - Pinkerton, K. E.
DA - JUL
DO - 10.1177/0192623316629804
IS - 5
PY - 2016
SN - 0192-6233
1533-1601
SP - 673-686
ST - Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over
Time
TI - Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over
Time
VL - 44
ID - 6111
ER -
TY - JOUR
AB - Silver nanoparticle (Ag NP) production methods are being developed and
refined to produce more uniform Ag NPs through chemical reactions involving silver
salt solutions, solvents, and capping agents to control particle formation. These
chemical reactants are often present as contaminants and/or coatings on the Ag NPs,
which could alter their interactions in vivo. To determine pulmonary effects of
citrate-coated Ag NPs, Sprague-Dawley rats were exposed once nose-only to
aerosolized Ag NPs (20 nm [C20] or 110 nm [C110] Ag NPs) for 6 hr. Bronchoalveolar
lavage fluid (BALF) and lung tissues were obtained at 1, 7, 21, and 56 days
postexposure for analyses. Inhalation of Ag NPs, versus citrate buffer control,
produced significant inflammatory and cytotoxic responses that were measured in
BALF cells and supernatant. At day 7, total cells, protein, and lactate
dehydrogenase were significantly elevated in BALF, and peak histopathology was
noted after C20 or C110 exposure versus control. At day 21, BALF polymorphonuclear
cells and tissue inflammation were significantly greater after C20 versus C110
exposure. By day 56, inflammation was resolved in Ag NP-exposed animals. Overall,
results suggest delayed, short-lived inflammatory and cytotoxic effects following
C20 or C110 inhalation and potential for greater responses following C20 exposure.
© 2016 The Author(s).
AU - Silva, R. M.
AU - Peake, J.
AU - Edwards, P. C.
AU - Patchin, E. S.
AU - Guo, T.
AU - Gordon, T.
AU - Chen, L. C.
AU - Sun, X.
DB - Scopus
DO - 10.1177/0192623316629804
IS - 5
KW - engineered nanomaterial
inflammation
inhalation exposure
pulmonary toxicity
Administration, Inhalation
Animals
Lung
Male
Metal Nanoparticles
Particle Size
Rats
Silver
citric acid
silver nanoparticle
metal nanoparticle
silver
aerosol
animal experiment
Article
controlled study
cytotoxicity
exposure
histopathology
lung parenchyma
lung toxicity
macrophage
male
nonhuman
oxidative stress
priority journal
rat
animal
drug effects
lung
particle size
pathology
Sprague Dawley rat
M3 - Article
PY - 2016
SP - 673-686
ST - Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over
Time
T2 - Toxicologic Pathology
TI - Aerosolized Silver Nanoparticles in the Rat Lung and Pulmonary Responses over
Time
84975497698&doi=10.1177%2f0192623316629804&partnerID=40&md5=77f44f12ba1279d3946e187
c3774a3d6
VL - 44
ID - 5504
ER -
TY - JOUR
AB - Background: Silver nanowires (Ag NWs) are increasingly being used to produce
touchscreens for smart phones and computers. When applied in a thin film over a
plastic substrate, Ag NWs create a transparent, highly-conductive network of fibers
enabling the touch interface between consumers and their electronics. Large-scale
application methods utilize techniques whereby Ag NW suspensions are deposited onto
substrates via droplets. Aerosolized droplets increase risk of occupational Ag NW
exposure. Currently, there are few published studies on Ag NW exposure-related
health effects. Concerns have risen about the potential for greater toxicity from
exposure to high-aspect ratio nanomaterials compared to their non-fibrous
counterparts. This study examines whether Ag NWs of varying lengths affect
biological responses and silver distribution within the lungs at different time-
points. Methods: Two different sizes of Ag NWs (2 mu m [S-Ag NWs] and 20 mu m [L-Ag
NWs]) were tested. Male, Sprague-Dawley rats were intratracheally instilled with Ag
NWs (0, 0.1, 0.5, or 1.0 mg/kg). Broncho-alveolar lavage fluid (BALF) and lung
tissues were obtained at 1, 7, and 21 days post exposure for analysis of BAL total
cells, cell differentials, and total protein as well as tissue pathology and silver
distribution. Results and conclusions: The two highest doses produced significant
increases in BAL endpoints. At Day 1, Ag NWs increased total cells, inflammatory
polymorphonuclear cells (PMNs), and total protein. PMNs persisted for both Ag NW
types at Day 7, though not significantly so, and by Day 21, PMNs appeared in line
with sham control values. Striking histopathological features associated with Ag
NWs included 1) a strong influx of eosinophils at Days 1 and 7; and 2) formation of
Langhans and foreign body giant cells at Days 7 and 21. Epithelial sloughing in the
terminal bronchioles (TB) and cellular exudate in alveolar regions were also
common. By Day 21, Ag NWs were primarily enclosed in granulomas or surrounded by
numerous macrophages in the TB-alveolar duct junction. These findings suggest short
and long Ag NWs produce pulmonary toxicity; thus, further research into exposure-
related health effects and possible exposure scenarios are necessary to ensure
human safety as Ag NW demand increases.
AN - WOS:000345871400001
AU - Silva, R. M.
AU - Xu, J. Y.
AU - Saiki, C.
AU - Franzi, L. M.
AU - Vulpe, C. D.
AU - Gilbert, B.
C7 - 52
DA - OCT 8
DO - 10.1186/s12989-014-0052-6
PY - 2014
SN - 1743-8977
ST - Short versus long silver nanowires: a comparison of in vivo pulmonary effects
post instillation
TI - Short versus long silver nanowires: a comparison of in vivo pulmonary effects
post instillation
VL - 11
ID - 6269
ER -
TY - JOUR
AB - Background: Silver nanowires (Ag NWs) are increasingly being used to produce
touchscreens for smart phones and computers. When applied in a thin film over a
plastic substrate, Ag NWs create a transparent, highly-conductive network of fibers
enabling the touch interface between consumers and their electronics. Large-scale
application methods utilize techniques whereby Ag NW suspensions are deposited onto
substrates via droplets. Aerosolized droplets increase risk of occupational Ag NW
exposure. Currently, there are few published studies on Ag NW exposure-related
health effects. Concerns have risen about the potential for greater toxicity from
exposure to high-aspect ratio nanomaterials compared to their non-fibrous
counterparts. This study examines whether Ag NWs of varying lengths affect
biological responses and silver distribution within the lungs at different time-
points. Methods: Two different sizes of Ag NWs (2 μm [S-Ag NWs] and 20 μm [L-Ag
NWs]) were tested. Male, Sprague- Dawley rats were intratracheally instilled with
Ag NWs (0, 0.1, 0.5, or 1.0 mg/kg). Broncho-alveolar lavage fluid (BALF) and lung
tissues were obtained at 1, 7, and 21 days post exposure for analysis of BAL total
cells, cell differentials, and total protein as well as tissue pathology and silver
distribution. Results and conclusions: The two highest doses produced significant
increases in BAL endpoints. At Day 1, Ag NWs increased total cells, inflammatory
polymorphonuclear cells (PMNs), and total protein. PMNs persisted for both Ag NW
types at Day 7, though not significantly so, and by Day 21, PMNs appeared in line
with sham control values. Striking histopathological features associated with Ag
NWs included 1) a strong influx of eosinophils at Days 1 and 7; and 2) formation of
Langhans and foreign body giant cells at Days 7 and 21. Epithelial sloughing in the
terminal bronchioles (TB) and cellular exudate in alveolar regions were also
common. By Day 21, Ag NWs were primarily enclosed in granulomas or surrounded by
numerous macrophages in the TB-alveolar duct junction. These findings suggest short
and long Ag NWs produce pulmonary toxicity; thus, further research into exposure-
related health effects and possible exposure scenarios are necessary to ensure
human safety as Ag NW demand increases. © 2014 Silva et al.
AU - Silva, R. M.
AU - Xu, J.
AU - Saiki, C.
AU - Franzi, L. M.
AU - Vulpe, C. D.
AU - Gilbert, B.
C7 - 52
DB - Scopus
DO - 10.1186/s12989-014-0052-6
IS - 1
KW - Histopathology
In vivo
Macrophage
Pulmonary toxicity
Silver nanowires
Animals
Inhalation Exposure
Lung
Male
Nanowires
Particle Size
Pneumonia
Risk Assessment
Silver
Time Factors
nanowire
protein
silver nanowire
unclassified drug
silver
animal cell
animal experiment
animal tissue
Article
bronchiole
controlled study
histopathology
in vivo study
lung alveolus
lung parenchyma
lung toxicity
macrophage
male
nonhuman
physical chemistry
priority journal
rat
Sprague Dawley rat
adverse effects
animal
chemically induced
chemistry
comparative study
cytology
dose response
drug effects
exposure
immunology
lung
metabolism
particle size
pathology
pneumonia
risk assessment
time
M3 - Article
PY - 2014
ST - Short versus long silver nanowires: A comparison of in vivo pulmonary effects
post instillation
TI - Short versus long silver nanowires: A comparison of in vivo pulmonary effects
post instillation
84964314526&doi=10.1186%2fs12989-014-0052-
6&partnerID=40&md5=bf682a1ee5f6ba2b63d4c28054cd7b8f
VL - 11
ID - 5628
ER -
TY - JOUR
AB - Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most widely used
drugs for the suppression of inflammation and pain. However, the analgesic
properties of NSAIDs are also associated with significant negative side effects,
most notably in the gastrointestinal (GI) tract. Increasingly, evidence indicates
that the ulcerogenic properties of some NSAIDs are not exclusively the result of
inhibition of cyclooxygenase isoforms in the GI tract, and other mechanisms,
including inhibition of cell migration and epithelial restitution, are being
explored. Recently, microarray analysis was used to identify potential novel
targets of NSAID activity in intestinal epithelial cells. Treated cells exhibited
significant reductions in the gene expression of pleiotrophin (PTN), a cytokine and
growth factor known to participate in angiogenesis and bone growth. This report
aimed to confirm the microarray results reported previously, and to measure protein
expression of PTN in intestinal epithelial cells. Furthermore, we also examined the
effects of exogenous PTN on cell migration in the presence and absence of either
NSAIDs with variable ulcerogenic potential or PTN-specific siRNA. Our results
demonstrated that indomethacin and NS-398, two NSAIDs with ulcerogenic potential
significantly decrease both gene and protein expressions of PTN in IEC-6 cells and
protein expression in IEC-6-Cdx2 cells. Additionally, cell migration experiments
with PTN siRNA showed that PTN is an important mediator of IEC-6 cell migration,
and addition of exogenous PTN partially restores the deficits in cell migration
caused by treatment with indomethacin and NS-398. Finally, measurement of PTN
protein expression in the GI tract of horses treated with phenylbutazone showed
that PTN expression is reduced by NSAIDs in vivo. Our results show that PTN is an
important mediator of cell migration in IEC-6 cells, and PTN is a potential target
through which NSAIDs may inhibit cell migration, epithelial restitution, and wound
healing in the GI tract.
AN - WOS:000308119300005
AU - Silver, K.
AU - Desormaux, A.
AU - Freeman, L. C.
AU - Lillich, J. D.
DA - AUG
DO - 10.3109/08977194.2012.693920
IS - 4
PY - 2012
SN - 0897-7194
1029-2292
SP - 258-266
ST - Expression of pleiotrophin, an important regulator of cell migration, is
inhibited in intestinal epithelial cells by treatment with non-steroidal anti-
inflammatory drugs
T2 - GROWTH FACTORS
TI - Expression of pleiotrophin, an important regulator of cell migration, is
inhibited in intestinal epithelial cells by treatment with non-steroidal anti-
inflammatory drugs
VL - 30
ID - 6214
ER -
TY - JOUR
AB - Non-steroidal anti-inflammatory drugs (NSAIDs) are used frequently worldwide
for the alleviation of pain despite their capacity to cause adverse
gastrointestinal (GI) side effects. GI toxicity, once thought to be the result of
non-specific inhibition of cyclooxegenase (COX) enzymes, is now hypothesized to
have multiple other causes that are COX independent. In particular, NSAIDs inhibit
intestinal epithelial restitution, the process by which barrier function in
intestinal mucosa is restored at sites of epithelial wounds within hours through
cell spreading and migration. Accordingly, recent evidence indicates that the
expression of calpain proteases, which play a key role in cell migration, is
decreased by NSAIDs that inhibit cell migration in intestinal epithelial cells
(IEC). Here, we examine the effect of NSAIDs on calpain activity and membrane
expression in IEC-6 cells. Indomethacin. NS-398, and SC-560 inhibited calpain
activity and decreased expression of calpain 2 in total membrane fractions and in
plasma membranes involved in cell attachment to the substrate. Additionally, we
demonstrated that inhibition of calpain activity by NSAIDs or ALLM, a calpain
inhibitor, limits cell migration and in vitro wound healing of IEC-6 cells. Our
results indicate that NSAIDs may inhibit cell migration by decreasing calpain
activity and membrane-associated expression of calpain 2. Our results provide
valuable insight into the mechanisms behind NSAID-induced GI toxicity and provide a
potential pathway through which these negative side effects can be avoided in
future members of the NSAID class. (C) 2010 Elsevier Ltd. All rights reserved.
AN - WOS:000284742100019
AU - Silver, K.
AU - Leloup, L.
AU - Freeman, L. C.
AU - Wells, A.
AU - Lillich, J. D.
DA - DEC
DO - 10.1016/j.biocel.2010.09.007
IS - 12
PY - 2010
SN - 1357-2725
1878-5875
SP - 2030-2036
ST - Non-steroidal anti-inflammatory drugs inhibit calpain activity and membrane
localization of calpain 2 protease
T2 - INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
TI - Non-steroidal anti-inflammatory drugs inhibit calpain activity and membrane
localization of calpain 2 protease
VL - 42
ID - 6177
ER -
TY - JOUR
AB - Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for the
alleviation of pain and inflammation, but these drugs are also associated with a
suite of negative side effects. Gastrointestinal (GI) toxicity is particularly
concerning since it affects an estimated 70% of individuals taking NSAIDs
routinely, and evidence suggests the majority of toxicity is occurring in the small
intestine. Traditionally, NSAID-induced GI toxicity has been associated with
indiscriminate inhibition of cyclooxygenase isoforms, but other mechanisms,
including inhibition of cell migration, intestinal restitution, and wound healing,
are likely to contribute to toxicity. Previous efforts demonstrated that treatment
of cultured intestinal epithelial cells (IEC) with NSAIDs inhibits expression and
activity of calpain proteases, but the effects of specific inhibition of calpain
expression in vitro or the effects of NSAIDs on intestinal cell migration in vivo
remain to be determined. Accordingly, we examined the effect of suppression of
calpain protease expression with siRNA on cell migration in cultured IECs and
evaluated the effects of NSAID treatment on epithelial cell migration and calpain
protease expression in rat duodenum. Our results show that calpain siRNA inhibits
protease expression and slows migration in cultured IECs. Additionally, NSAID
treatment of rats slowed migration up the villus axis and suppressed calpain
expression in duodenal epithelial cells. Our results are supportive of the
hypothesis that suppression of calpain expression leading to slowing of cell
migration is a potential mechanism through which NSAIDs cause GI toxicity.
AN - WOS:000402496800001
AU - Silver, K.
AU - Littlejohn, A.
AU - Thomas, L.
AU - Bawa, B.
AU - Lillich, J. D.
DA - MAY 15
DO - 10.1016/j.tox.2017.03.017
PY - 2017
SN - 0300-483X
SP - 1-12
ST - Suppression of calpain expression by NSAIDs is associated with inhibition of
cell migration in rat duodenum
T2 - TOXICOLOGY
TI - Suppression of calpain expression by NSAIDs is associated with inhibition of
cell migration in rat duodenum
VL - 383
ID - 6391
ER -
TY - JOUR
AB - Clinical use of non-steroidal anti-inflammatory drugs (NSAIDs) is well known
to cause gastrointestinal ulcer formation via several mechanisms that include
inhibiting epithelial cell migration and mucosal restitution. The drug-affected
signaling pathways that contribute to inhibition of migration by NSAIDs are poorly
understood, though previous studies have shown that NSAIDs depolarize membrane
potential and suppress expression of calpain proteases and voltage-gated potassium
(K-v) channel subunits. K-v channels play significant roles in cell migration and
are targets of NSAID activity in white blood cells, but the specific functional
effects of NSAID-induced changes in K-v channel expression, particularly on cell
migration, are unknown in intestinal epithelial cells. Accordingly, we investigated
the effects of NSAIDs on expression of K(v)1.3, 1.4, and 1.6 in vitro and/or in
vivo and evaluated the functional significance of loss of K-v subunit expression.
Indomethacin or NS-398 reduced total and plasma membrane protein expression of
K(v)1.3 in cultured intestinal epithelial cells (IEC-6). Additionally,
depolarization of membrane potential with margatoxin (MgTx), 40 mM K+, or silencing
of K-v channel expression with siRNA significantly reduced IEC-6 cell migration and
disrupted calpain activity. Furthermore, in rat small intestinal epithelia,
indomethacin and NS-398 had significant, yet distinct, effects on gene and protein
expression of K(v)1.3, 1.4, or 1.6, suggesting that these may be clinically
relevant targets. Our results show that inhibition of epithelial cell migration by
NSAIDs is associated with decreased expression of K-v channel subunits, and provide
a mechanism through which NSAIDs inhibit cell migration and may contribute to
NSAID-induced gastrointestinal (GI) toxicity. (C) 2015 Elsevier Inc. All rights
reserved.
AN - WOS:000366150300007
AU - Silver, K.
AU - Littlejohn, A.
AU - Thomas, L.
AU - Marsh, E.
AU - Lillich, J. D.
DA - DEC 15
DO - 10.1016/j.bcp.2015.10.017
IS - 4
PY - 2015
SN - 0006-2952
1873-2968
SP - 614-628
ST - Inhibition of K-v channel expression by NSAIDs depolarizes membrane potential
and inhibits cell migration by disrupting calpain signaling
T2 - BIOCHEMICAL PHARMACOLOGY
TI - Inhibition of K-v channel expression by NSAIDs depolarizes membrane potential
and inhibits cell migration by disrupting calpain signaling
VL - 98
ID - 6480
ER -
TY - JOUR
AB - This review covers the most recent developments of inorganic and organic-
inorganic composite coatings for orthopedic implants, providing the interface with
living tissue and with potential for drug delivery to combat infections.
Conventional systemic delivery of drugs is an inefficient procedure that may cause
toxicity and may require a patient's hospitalization for monitoring. Local delivery
of antibiotics and other bioactive molecules maximizes their effect where they are
required, reduces potential systemic toxicity and increases timeliness and cost
efficiency. In addition, local delivery has broad applications in combating
infection-related diseases. Polymeric coatings may present some disadvantages.
These disadvantages include limited chemical stability, local inflammatory
reactions, uncontrolled drug-release kinetics, late thrombosis and restenosis. As a
result, embedding of bioactive compounds and biomolecules within inorganic coatings
(bioceramics, bioactive glasses) is attracting significant attention. Recently
nanoceramics have attracted interest because surface nanostructuring allows for
improved cellular adhesion, enhances osteoblast proliferation and differentiation,
and increases biomineralization. Organic-inorganic composite coatings, which
combine biopolymers and bioactive ceramics that mimick bone structure to induce
biomineralization, with the addition of biomolecules, represent alternative systems
and ideal materials for "smart" implants. In this review, emphasis is placed on
materials and processing techniques developed to advance the therapeutic use of
biomolecules-eluting coatings, based on nanostructured ceramics. One part of this
report is dedicated to inorganic and composite coatings with antibacterial
functionality. From the Clinical Editor: Inorganic and composite nanotechnology-
based coating methods have recently been developed for orthopedic applications,
with the main goal to provide bactericide and other enhanced properties, which may
result in reduced need for pharmaceutical interventions and overall more cost
effective orthopedic procedures. This review discusses key aspects of the above
developments. © 2011 Elsevier Inc.
AU - Simchi, A.
AU - Tamjid, E.
AU - Pishbin, F.
AU - Boccaccini, A. R.
DB - Scopus
DO - 10.1016/j.nano.2010.10.005
IS - 1
KW - Antimicrobial
Ceramics
Coatings
Composites
Drug-eluting implants
Nanostructures
Humans
Nanotechnology
Bactericides
Bioactive glass
Bioceramics
Biomineralization
Biomolecules
Biopolymers
Chemical stability
Cost effectiveness
Cost reduction
Drug delivery
Inorganic coatings
Mammals
Microorganisms
Ocean habitats
Organic coatings
Toxicity
antibiotic agent
bioceramics
biomaterial
biopolymer
cefalotin
cefamandole
doxorubicin
drug carrier
gentamicin
glass
hydroxyapatite
inorganic compound
macrogol
nanocoating
nanocomposite
nanofilm
nitric oxide
organic compound
polyglactin
polylactic acid
silver
titanium dioxide
tobramycin
vancomycin
Alternative systems
Bioactive ceramic
Bioactive compounds
Bioactive molecules
Bone structure
Broad application
Cellular adhesion
Coating methods
Cost effective
Cost efficiency
Living tissues
Local delivery
Nano ceramics
Nanostructured ceramic
Organic-inorganic composite
Orthopaedic applications
Orthopedic implant
Osteoblast proliferation
Polymeric coatings
Processing technique
Recent progress
Release kinetics
Restenosis
Surface nanostructuring
Therapeutic use
biomineralization
cell adhesion
cell proliferation
composite material
cost
human
material coating
nonhuman
orthopedic equipment
osteoblast
review
Composite coatings
M3 - Review
PY - 2011
SP - 22-39
ST - Recent progress in inorganic and composite coatings with bactericidal
capability for orthopaedic applications
TI - Recent progress in inorganic and composite coatings with bactericidal
capability for orthopaedic applications
78751697635&doi=10.1016%2fj.nano.2010.10.005&partnerID=40&md5=eab8583a51916c27e842b
39ac9d5e28a
VL - 7
ID - 5687
ER -
TY - JOUR
AB - The highly conserved matrix protein 2 (M2) is a good candidate for the
development of a broadly protective influenza vaccine that induces long-lasting
immunity. In animal models, natural killer (NK) cells have been proposed to play an
important role in the protection provided by M2-based vaccines through a mechanism
of antibody-dependent cell-mediated cytotoxicity (ADCC). We investigated the
ability of the human anti-M2 Ab1-10 monoclonal antibody (mAb) to activate human NK
cells. They mediated ADCC against M2-expressing cells in the presence of Ab1-10
mAb. Furthermore, NK cell pro-inflammatory cytokine and chemokine secretion is also
enhanced when Ab1-10 mAb is present. We also generated cytokine-preactivated NK
cells and showed that they still displayed increased effector functions in the
presence of Ab1-10 mAb. Thus, our study has demonstrated that human resting and
cytokine-preactivated NK cells may have a very important role in the protection
provided by anti-M2 Abs.
AN - WOS:000353659100060
AU - Simhadri, V. R.
AU - Dimitrova, M.
AU - Mariano, J. L.
AU - Zenarruzabeitia, O.
AU - Zhong, W. M.
AU - Ozawa, T.
AU - Muraguchi, A.
AU - Kishi, H.
AU - Eichelberger, M. C.
AU - Borrego, F.
C7 - e0124677
DA - APR 27
DO - 10.1371/journal.pone.0124677
IS - 4
PY - 2015
SN - 1932-6203
ST - A Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated
Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated
Natural Killer (NK) Cells
T2 - PLOS ONE
TI - A Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated
Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated
Natural Killer (NK) Cells
VL - 10
ID - 6441
ER -
TY - JOUR
AB - The present study describes potential beneficial and adverse effects of
plant-extract synthesized gold nanoparticles (AuNPs) on ethanol toxicity in SH-SY5Y
cells. Although kudzu root extract (K), edible-gum extract (G), alone or in
combination (KG), reduced Au3+ into AuNPs, the extract's composition and the
reaction temperature determined their size (AuNPKG(90<50<37) << AuNPK (90,50<37) <
AuNPG (90<50); the subscript KG, K, or G is extract identification and numerical
vales are reaction temperature in Celsius) and biological properties (AuNPKG
(90,50>37) << AuNPK ((90,50>37)) < AuNPG (90,50)). The surface of each AuNP
contained the extract's active ingredients, that were analyzed and confirmed using
laser desorption ionization (LDI)) and low-matrix laser desorption-ionization
((L)MALDI). AuNPKG-50 was (i) least toxic to SH-SY5Y cells, but most effective in
suppressing the adverse effects of ethanol on SH-SY5Y cells, and (ii) more
effective than a combination of free kudzu and gum extracts. The beneficial and
adverse effects of AuNPs may have been modified by the formation of proteins
corona. This study provides a proof of concept for possible application of plant-
extract synthesized AuNPs in mitigating ethanol toxicity.
AN - WOS:000425227000013
AU - Singh, A. K.
C7 - 70
DA - DEC
DO - 10.3390/biomedicines5040070
IS - 4
PY - 2017
SN - 2227-9059
ST - Comparative Therapeutic Effects of Plant-Extract Synthesized and
Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory
Activity in SH-SY5Y Cells In Vitro
T2 - BIOMEDICINES
TI - Comparative Therapeutic Effects of Plant-Extract Synthesized and
Traditionally Synthesized Gold Nanoparticles on Alcohol-Induced Inflammatory
Activity in SH-SY5Y Cells In Vitro
VL - 5
ID - 6447
ER -
TY - JOUR
AB - Rheumatoid arthritis (RA) is a major global public health challenge, and
novel therapies are required to combat it. Silver nanoparticles (AgNPs) have been
employed as delivery vehicles of anti-inflammatory drugs for RA therapy, and it has
been recently realized that AgNPs have anti-inflammatory action on their own.
However, their conventional synthesis processes might result in cytotoxicity and
environmental hazards. Instead, the use of natural products as a reducing and
stabilizing agent in the biosynthesis of silver nanoparticles has arisen as an
option to decrease the cytotoxic and environmental concerns associated with
chemical synthesis of AgNPs. In this study, we challenged the efficacy of
Commiphora mukul (guggul) aqueous extract as a reducing and/or capping agent for
the biosynthesis of AgNPs. Guggul-mediated biosynthesized silver nanoparticles (G-
AgNPs) were characterized via UV-vis spectroscopy, dynamic light scattering, and
scanning electron microscopy. In addition, their anti-arthritic potential was
evaluated in an adjuvant-induced arthritis (AIA) model. The fabricated NPs showed
an absorption peak at 412 nm, corresponding to the typical surface plasmon
resonance band of AgNPs. The synthesized G-AgNPs were nearly spherical, with a
particle size of 337.6 ± 12.1 nm and a negative surface charge (−18.9 ± 1.8 mV). In
AIA rat model, synthesized G-AgNPs exerted a potent anti-inflammatory action, as
manifested by a remarkable reduction in paw volume (>40%) along with elicitation of
a minimal arthritic score, compared to control rats. In addition, when compared to
arthritic rats, treatment with G-AgNPs efficiently restored the activity of
antioxidant enzyme, superoxide dismutase, and catalase, indicating the efficiency
of synthesized G-AgNPs in alleviating the oxidative stress associated with RA.
Finally, histological examination revealed comparatively lower inflammatory cells
infiltration in ankle joint tissue upon treatment with G-AgNPs. Collectively,
biosynthesized G-AgNPs might represent a plausible therapeutic option for the
management of RA. © 2022 by the authors.
AU - Singh, A.
AU - Boregowda, S. S.
AU - Moin, A.
AU - Abu Lila, A. S.
AU - Aldawsari, M. F.
AU - Khafagy, E. S.
AU - Alotaibi, H. F.
AU - Jayaramu, R. A.
C7 - 2318
DB - Scopus
IS - 11
arthritic score
guggul
catalase
Commiphora mukul extract
methotrexate
plant extract
reducing agent
silver nanoparticle
unclassified drug
adjuvant arthritis
adult
animal experiment
animal model
animal tissue
antiarthritic activity
Article
behavior change
cell infiltration
controlled study
drug activity
drug determination
drug efficacy
drug synthesis
enzyme activity
female
inflammatory cell
nonhuman
oxidative stress
particle size
pH measurement
phytochemistry
rat
surface charge
toxicity testing
zeta potential
M3 - Article
PY - 2022
ST - Biosynthesis of Silver Nanoparticles Using Commiphora mukul Extract:
Evaluation of Anti-Arthritic Activity in Adjuvant-Induced Arthritis Rat Model
T2 - Pharmaceutics
TI - Biosynthesis of Silver Nanoparticles Using Commiphora mukul Extract:
85141828599&doi=10.3390%2fpharmaceutics14112318&partnerID=40&md5=770af7367076014e9a
5c2d6c2fea7f6c
VL - 14
ID - 4966
ER -
TY - JOUR
AB - Rheumatoid arthritis (RA) is a major global public health challenge, and
novel therapies are required to combat it. Silver nanoparticles (AgNPs) have been
employed as delivery vehicles of anti-inflammatory drugs for RA therapy, and it has
been recently realized that AgNPs have anti-inflammatory action on their own.
However, their conventional synthesis processes might result in cytotoxicity and
environmental hazards. Instead, the use of natural products as a reducing and
stabilizing agent in the biosynthesis of silver nanoparticles has arisen as an
option to decrease the cytotoxic and environmental concerns associated with
chemical synthesis of AgNPs. In this study, we challenged the efficacy of
Commiphora mukul (guggul) aqueous extract as a reducing and/or capping agent for
the biosynthesis of AgNPs. Guggul-mediated biosynthesized silver nanoparticles (G-
AgNPs) were characterized via UV-vis spectroscopy, dynamic light scattering, and
scanning electron microscopy. In addition, their anti-arthritic potential was
evaluated in an adjuvant-induced arthritis (AIA) model. The fabricated NPs showed
an absorption peak at 412 nm, corresponding to the typical surface plasmon
resonance band of AgNPs. The synthesized G-AgNPs were nearly spherical, with a
particle size of 337.6 +/- 12.1 nm and a negative surface charge (-18.9 +/- 1.8
mV). In AIA rat model, synthesized G-AgNPs exerted a potent anti-inflammatory
action, as manifested by a remarkable reduction in paw volume (>40%) along with
elicitation of a minimal arthritic score, compared to control rats. In addition,
when compared to arthritic rats, treatment with G-AgNPs efficiently restored the
activity of antioxidant enzyme, superoxide dismutase, and catalase, indicating the
efficiency of synthesized G-AgNPs in alleviating the oxidative stress associated
with RA. Finally, histological examination revealed comparatively lower
inflammatory cells infiltration in ankle joint tissue upon treatment with G-AgNPs.
Collectively, biosynthesized G-AgNPs might represent a plausible therapeutic option
for the management of RA.
AN - WOS:000882646300001
AU - Singh, A.
AU - Boregowda, S. S.
AU - Moin, A.
AU - Abu Lila, A. S.
AU - Aldawsari, M. F.
AU - Khafagy, E.
AU - Alotaibi, H. F.
AU - Jayaramu, R. A.
C7 - 2318
DA - NOV
IS - 11
PY - 2022
SN - 1999-4923
ST - Biosynthesis of Silver Nanoparticles Using Commiphora mukul Extract:
T2 - PHARMACEUTICS
TI - Biosynthesis of Silver Nanoparticles Using Commiphora mukul Extract:
VL - 14
ID - 5858
ER -
TY - JOUR
AB - Recently, green metal nanoparticles have received global attention owing to
their economical synthesis, biocompatible nature, widespread biomedical and
environmental applications. Current study demonstrates a sustainable approach for
the green synthesis of silver nanoparticles (P-AgNPs) and gold nanoparticles (P-
AuNPs) from P. serrulata fresh fruit extract. The silver and gold nanoparticles
were synthesized in a very rapid, efficient and facile manner, within 50 min and 30
s at 80 °C, respectively. The nanoparticles were characterized by using visual
observation, UV–Vis, FE-TEM, EDX, elemental mapping, FT-IR, XRD and DLS, which
confirmed the formation of monodispersed, crystalline and stable nanoparticles.
Further, we explored these nanoparticles for anti-inflammatory activity through
inhibition of downstream NF-κB activation in macrophages (RAW264.7). We
demonstrated that the nanoparticles reduced expression of inflammatory mediators
such as nitric oxide (NO), prostaglandin E2 (PEG 2 ), inducible nitric oxide
synthase (iNOS) and cyclooxygenase-2 (COX-2) was attenuated in lipopolysaccharide
(LPS)-induced RAW264.7 cells. Furthermore, nanoparticles significantly suppressed
LPS-induced activation of NF-κB signalling pathway via p38 MAPK in RAW 264.7 cells.
To the best of our knowledge, this is the first report on the efficient green
synthesis of P-AgNPs and P-AuNPs using P. serrulata fresh fruit extract and its in
vitro anti-inflammatory effects. Collectively, our results suggest that P.
serrulata fresh fruit extract is a green resource for the eco-friendly synthesis of
P-AgNPs and P-AuNPs, which further can be utilized as a novel therapeutic agent for
prevention and cure of inflammation due to their biocompatible nature. © 2017, ©
AU - Singh, P.
AU - Ahn, S.
AU - Kang, J. P.
AU - Veronika, S.
AU - Huo, Y.
AU - Singh, H.
AU - Chokkaligam, M.
AU - El-Agamy Farh, M.
AU - Aceituno, V. C.
AU - Kim, Y. J.
AU - Yang, D. C.
DB - Scopus
DO - 10.1080/21691401.2017.1408117
IS - 8
KW - gold nanoparticles
Green synthesis
inflammation
NF-κB
P. serrulata
Animals
Fruit
Gold
Metal Nanoparticles
Mice
Nanospheres
Plant Extracts
Prunus
RAW 264.7 Cells
Silver
Biocompatibility
Cell signaling
Chemical activation
Fiber optic sensors
Fruits
Gold nanoparticles
Nitric oxide
Pathology
cyclooxygenase 2
gold nanoparticle
lipopolysaccharide
nitric oxide
prostaglandin E2
silver nanoparticle
gold
metal nanoparticle
nanosphere
plant extract
silver
Environmental applications
Inducible nitric oxide synthase (iNOS)
Silver and gold nanoparticles
Spherical silver nanoparticles
Article
cell viability
drug synthesis
enzyme activation
fruit
in vitro study
macrophage
nanomedicine
Prunus serrulata
signal transduction
animal
chemistry
drug effect
MAPK signaling
mouse
RAW 264.7 cell line
Metal nanoparticles
M3 - Article
PY - 2018
SP - 2022-2032
ST - In vitro anti-inflammatory activity of spherical silver nanoparticles and
monodisperse hexagonal gold nanoparticles by fruit extract of Prunus serrulata: a
green synthetic approach
TI - In vitro anti-inflammatory activity of spherical silver nanoparticles and
monodisperse hexagonal gold nanoparticles by fruit extract of Prunus serrulata: a
green synthetic approach
85035750338&doi=10.1080%2f21691401.2017.1408117&partnerID=40&md5=af479a91ca0fb7a5ce
48cf34ba1d5a1b
VL - 46
ID - 5371
ER -
TY - JOUR
AB - The present study investigates a simple and convenient one-step procedure for
the preparation of bovine serum albumin (BSA)-Rh2 nanoparticles (NPs) at room
temperature. In this work, ginsenoside Rh2 was entrapped within the BSA protein to
form BSA-Rh2 NPs to enhance the aqueous solubility, stability, and therapeutic
efficacy of Rh2. The physiochemical characterization by high-performance liquid
chromatography, nuclear magnetic resonance, Fourier transform infrared
spectroscopy, field emission transmission electron microscopy, dynamic light
scattering, and thermogravimetric analysis confirmed that the prepared BSA-Rh2 NPs
were spherical, highly monodispersed, and stable in aqueous systems. In addition,
the stability of NPs in terms of different time intervals, pHs, and temperatures
(20 degrees C-700 degrees C) was analyzed. The results obtained with different pHs
showed that the synthesized BSA-Rh2 NPs were stable in the physiological buffer (pH
7.4) for up to 8 days, but degraded under acidic conditions (pH 5.0) representing
the pH inside tumor cells. Furthermore, comparative analysis of the water
solubility of BSA-Rh2 NPs and standard Rh2 showed that the BSA nanocarrier enhanced
the water solubility of Rh2. Moreover, in vitro cytotoxicity assays including cell
viability assays and morphological analyses revealed that Rh2-entrapped BSA NPs,
unlike the free Rh2, demonstrated better in vitro cell viability in HaCaT skin cell
lines and that BSA enhanced the anticancer effect of Rh2 in A549 lung cell and HT29
colon cancer cell lines. Additionally, anti-inflammatory assay of BSA-Rh2 NPs and
standard Rh2 performed using RAW264.7 cells revealed decreased lipopolysaccharide-
induced nitric oxide production by BSA-Rh2 NPs. Collectively, the present study
suggests that BSA can significantly enhance the therapeutic behavior of Rh2 by
improving its solubility and stability in aqueous systems, and hence, BSA-Rh2 NPs
may potentially be used as a ginsenoside delivery vehicle in cancer and
inflammatory cell lines.
AN - WOS:000402292700001
AU - Singh, P.
AU - Kim, Y. J.
AU - Singh, H.
AU - Ahn, S.
AU - Castro-Aceituno, V.
AU - Yang, D. C.
DO - 10.2147/IJN.S125154
PY - 2017
SN - 1178-2013
SP - 4073-4084
ST - In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum
albumin nanoparticles: in vitro cytocompatibility studies
TI - In situ preparation of water-soluble ginsenoside Rh2-entrapped bovine serum
albumin nanoparticles: in vitro cytocompatibility studies
VL - 12
ID - 6596
ER -
TY - JOUR
AB - Previously, we showed the rapid and eco-friendly synthesis of gold and silver
nanoparticles within 3 and 45 min by fresh leaves extract of herbal medicinal plant
Panax ginseng. In addition, we characterized the nanoparticles in terms of shape,
size, morphology and stability by FE-TEM, EDX, elemental mapping, SEAD, XRD and
particles size analysis. In addition of this, we showed their antimicrobial, anti-
coagulant, and biofilm inhibition activity of nanoparticles. Continuing our
previous study, here we highlight the further characterization and biomedical
applications of P. ginseng leaf-mediated gold and silver nanoparticles. We
characterized the nanoparticles further in terms of active functional group and
capping layer, surface charge, and temperature stability. Based on these factors,
we explored the nanoparticles for antioxidant efficacy, biocompatibility in HaCaT
cells, 3T3-L1 pre-adipocytes cells, for anticancer efficacy in A549 lung cancer and
B16BL6 skin melenoma cancer cell lines and for anti-inflammation efficacy in RAW
264.7 cell lines. Based on our findings, we suggest that the P. ginseng-mediated
gold nanoparticles have high antioxidant activity and highly biocompatibility in
HaCaT cells, 3T3-L1 pre-adipocytes cells, RAW 264.7 cells lines and could be
considered for future drug delivery carriers. The silver nanoparticles also showed
high potent antioxidant efficacy, additionally it showed high anticancer effect in
A549 lung cancer and B16BL6 skin melenoma cancer cell lines as compared to
precursor salts. Moreover, both gold and silver nanoparticles have anti-
inflammatory efficacies in RAW 264.7 cells. Thus, the study may provide useful
insights of P. ginseng leaves extract-mediated biocompatible gold and silver
nanoparticles and improving their applicability in designing nanoparticles carrier
systems for drug delivery applications. © 2016 Informa UK Limited, trading as
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Jiménez, Z.
AU - Simu, S. Y.
AU - Kim, Y. J.
AU - Yang, D. C.
DB - Scopus
DO - 10.1080/21691401.2016.1243547
IS - 7
KW - anti-inflammation
anticancer
antioxidant
gold nanoparticles
Panax ginseng
3T3-L1 Cells
Animals
Biphenyl Compounds
Cell Survival
Chemistry Techniques, Synthetic
Gold
Humans
Materials Testing
Metal Nanoparticles
Mice
Nanotechnology
Nitric Oxide
Panax
Picrates
Plant Leaves
Silver
Antioxidants
Biocompatibility
Biological organs
Cell culture
Cells
Diseases
Fiber optic sensors
Metal nanoparticles
Pathology
Plant extracts
ginseng extract
gold nanoparticle
silver nanoparticle
1,1-diphenyl-2-picrylhydrazyl
biphenyl derivative
gold
metal nanoparticle
nitric oxide
picric acid
silver
Anti-inflammation
Drug delivery applications
Drug delivery carrier
3T3-L1 cell line
A-549 cell line
adipocyte
anticoagulation
Article
B16-BL6 cell line
biocompatibility
biofilm
cell viability
controlled study
drug efficacy
drug stability
drug synthesis
ginseng
HaCat cell line
in vitro study
nonhuman
particle size
plant leaf
RAW 264.7 cell line
surface charge
X ray diffraction
zeta potential
animal
biosynthesis
cell survival
chemistry
drug effect
human
materials testing
mouse
nanotechnology
synthesis
Gold nanoparticles
M3 - Article
PY - 2017
SP - 1415-1424
ST - Pharmacological importance, characterization and applications of gold and
silver nanoparticles synthesized by Panax ginseng fresh leaves
TI - Pharmacological importance, characterization and applications of gold and
84995673171&doi=10.1080%2f21691401.2016.1243547&partnerID=40&md5=98fe884f4802fb3e27
ee914035b363bc
VL - 45
ID - 5534
ER -
TY - JOUR
AB - Previously, we showed the rapid and eco-friendly synthesis of gold and silver
nanoparticles within 3 and 45min by fresh leaves extract of herbal medicinal plant
Panax ginseng. In addition, we characterized the nanoparticles in terms of shape,
size, morphology and stability by FE-TEM, EDX, elemental mapping, SEAD, XRD and
particles size analysis. In addition of this, we showed their antimicrobial, anti-
coagulant, and biofilm inhibition activity of nanoparticles. Continuing our
previous study, here we highlight the further characterization and biomedical
applications of P. ginseng leaf-mediated gold and silver nanoparticles. We
characterized the nanoparticles further in terms of active functional group and
capping layer, surface charge, and temperature stability. Based on these factors,
we explored the nanoparticles for antioxidant efficacy, biocompatibility in HaCaT
cells, 3T3-L1 pre-adipocytes cells, for anticancer efficacy in A549 lung cancer and
B16BL6 skin melenoma cancer cell lines and for anti-inflammation efficacy in RAW
264.7 cell lines. Based on our findings, we suggest that the P. ginseng-mediated
gold nanoparticles have high antioxidant activity and highly biocompatibility in
HaCaT cells, 3T3-L1 pre-adipocytes cells, RAW 264.7 cells lines and could be
considered for future drug delivery carriers. The silver nanoparticles also showed
high potent antioxidant efficacy, additionally it showed high anticancer effect in
A549 lung cancer and B16BL6 skin melenoma cancer cell lines as compared to
precursor salts. Moreover, both gold and silver nanoparticles have anti-
inflammatory efficacies in RAW 264.7 cells. Thus, the study may provide useful
insights of P. ginseng leaves extract-mediated biocompatible gold and silver
nanoparticles and improving their applicability in designing nanoparticles carrier
systems for drug delivery applications.
AN - WOS:000413975300020
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Jimenez, Z.
AU - Simu, S. Y.
AU - Kim, Y. J.
AU - Yang, D. C.
DO - 10.1080/21691401.2016.1243547
IS - 7
PY - 2017
SN - 2169-1401
2169-141X
SP - 1415-1424
ST - Pharmacological importance, characterization and applications of gold and
T2 - ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
TI - Pharmacological importance, characterization and applications of gold and
VL - 45
ID - 5913
ER -
TY - JOUR
AB - The current study highlights the fabrication of drug delivery system by
utilizing 200 nm mesoporous silica nanoparticles (MSNPs) with 4-nm pore size, as a
carrier system for delivery ginsenoside compound K (CK) and Rh2 to enhance their
efficacy. The two pharmacologically imperative ginsenosides, CK and Rh2, were
loaded to the MSNPs to prepare MSNPs-CK and MSNPs-Rh2, respectively. A fluorescein
isothiocyanate (FITC) fluorescent dye was combined in the MSNPs carrier system, in
order to trace the cellular uptake of ginsenoside-loaded nanoparticles for in vitro
studies. Following purification, the so-prepared MSNPs-CK-FITC and MSNPs-Rh2-FITC
were characterized by several analytical techniques, which includes, high-pressure
liquid chromatography (HPLC), H-1 NMR, field emission transmission electron
microscopy (FE-TEM), Fourier transform infrared spectroscopy (FT-IR), x-ray
diffraction (XRD), thermogravimetric analysis (TGA), and dynamic light scattering
(DLS). In vitro cytotoxicity assay in HaCaT skin cells, A549 lung cancer cells,
HepG2 liver carcinoma cells, and HT-29 colon cancer cell lines were tested for
MSNPs-CK-FITC and MSNPs-Rh2-FITC. The results demonstrate the excellent
biocompatibility of nanoparticles in normal cell lines (HaCaT skin cells) and
anticancer efficacy in all the tested cancer cell lines at 10-mu M concentration.
Additionally, the in vitro anti-inflammatory behavior of MSNPs-CK-FITC and MSNPs-
Rh2-FITC were checked in RAW264.7 (murine macrophage) cell lines. The outcomes
showed higher anti-inflammatory efficacy of MSNPs-CK-FITC and MSNPs-Rh2-FITC as
compared to standard ginsenosides CK and Rh2 in RAW264.7 cell lines. Thus, with 200
nm MSNPs carrier system for the delivery ginsenosides CK and Rh2, a high amount of
loading and increasing in vitro pharmacological efficacies of ginsenosides were
realized. This study may provide useful insights for designing and improving the
applicability of MSNPs for ginsenoside delivery.
AN - WOS:000406203500002
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Kim, Y. J.
AU - Farh, M. E.
AU - Yang, D. C.
C7 - 257
DA - JUL 20
DO - 10.1007/s11051-017-3949-9
IS - 7
PY - 2017
SN - 1388-0764
1572-896X
ST - Engineering of mesoporous silica nanoparticles for release of ginsenoside CK
and Rh2 to enhance their anticancer and anti-inflammatory efficacy: in vitro
studies
TI - Engineering of mesoporous silica nanoparticles for release of ginsenoside CK
and Rh2 to enhance their anticancer and anti-inflammatory efficacy: in vitro
studies
VL - 19
ID - 6235
ER -
TY - JOUR
AB - Ginsenosides are triterpenoids that are found in P. ginseng; they have
numerous important structural, functional and pharmacological properties. In this
work, a desolvation method was used to entrap ginsenoside CK within bovine serum
albumin (BSA) to form BSA-CK nanoparticles (NPs), which enhance its aqueous
solubility and stability. Following purification, the BSA-CK NPs were characterized
by several physico-chemical techniques, including high pressure liquid
chromatography (HPLC), electrophoresis, H-1 NMR spectroscopy, Fourier transform
infrared spectroscopy (FT-IR), field emission transmission electron microscopy (FE-
TEM), zeta potential, particle size analysis by dynamic light scattering (DLS), and
thermogravimetric analysis (TGA); the results confirm that the as-prepared BSA-CK
NPs are spherical, highly monodispersed and stable in aqueous systems. In addition,
the time-dependent and pH stabilities of the BSA-CK NPs were analyzed over a period
of 8 days; the results suggest that the nanoparticles are stable in physiological
buffer (pH 7.4), whereas they are readily degraded under acidic conditions (pH 5.0)
which mimic intracellular pH conditions. Furthermore, comparative water solubility
analysis of the BSA-CK NPs and standard CK showed that the BSA carrier enhances the
water solubility of ginsenoside CK. In vitro cytotoxicity assays of the BSA-CK NPs
and standard CK revealed that the BSA-CK NPs demonstrate greater in vitro
therapeutic efficacy in the HaCaT skin cell line, the A549 lung cancer cell line,
the HepG2 liver carcinoma cell line and the HT29 colon cancer cell line in
comparison with standard CK. Moreover, RAW264.7 cells treated with BSA-CK NPs
exhibited decreased lipopolysaccharide-induced NO production. Collectively, these
results suggest that the BSA-CK NPs may be useful as a delivery vehicle in cancer
cell lines and may also possess anti-inflammatory effects.
AN - WOS:000396290400052
AU - Singh, P.
AU - Singh, H.
AU - Ahn, S.
AU - Kim, Y. J.
AU - Yang, D. C.
DO - 10.1039/c6ra25264h
IS - 25
PY - 2017
SN - 2046-2069
SP - 15397-15407
ST - Bovine serum albumin as a nanocarrier for the efficient delivery of
ginsenoside compound K: preparation, physicochemical characterizations and in vitro
biological studies
T2 - RSC ADVANCES
TI - Bovine serum albumin as a nanocarrier for the efficient delivery of
ginsenoside compound K: preparation, physicochemical characterizations and in vitro
biological studies
VL - 7
ID - 6708
ER -
TY - JOUR
AB - Ideal dressing materials for complex and large asymmetric burns should have
the dual properties of anti-bacterial and regenerative with advanced applicability
of direct deposit on the wound at the patient bedside. In this study, core-shell
nanofibers (polycaprolactone; PCL and polyethylene oxide; PEO) with different
percent of silver sulfadiazine (SSD) loading (2–10%) were prepared by the
airbrushing method using a custom build device. Results indicate a sustained
release profile of silver sulfadiazine (SSD) up to 28 days and concentration-
dependent anti-bacterial activity. The morphology and proliferation of human dermal
fibroblast (HDF) cells and human dental follicle stem cells (HDFSC) on the silver
sulfadiazine loaded nanofibers confirm the biocompatibility of airbrushed
nanofibers. Moreover, upregulation of extracellular matrix (ECM) proteins (Col I,
Col III, and elastin) support the differentiation and regenerative properties of
silver sulfadiazine nanofiber mats. This was further confirmed by the complete
recovery of rabbit burn wound models within 7 days of silver sulfadiazine loaded
nanofiber dressing. Histopathology data show silver sulfadiazine loaded core-shell
nanofibers' anti-inflammatory and proliferative activity without any adverse
response on the tissue. Overall data display that the airbrushed silver
sulfadiazine-loaded core-shell nanofibers are effective dressing material with the
possibility of direct fiber deposition on the wound to cover, heal, and regenerate
large asymmetric burn wounds. © 2021
AU - Singh, R.
AU - Roopmani, P.
AU - Chauhan, M.
AU - Basu, S. M.
AU - Deeksha, W.
AU - Kazem, M. D.
AU - Hazra, S.
AU - Rajakumara, E.
AU - Giri, J.
C7 - 121358
DB - Scopus
DO - 10.1016/j.ijpharm.2021.121358
KW - Anti-bacterial activity
Burn wound healing
Coaxial airbrushing
Core-shell nanofibers
Silver sulfadiazine
Animals
Bandages
Burns
Humans
Nanofibers
Rabbits
Silver Sulfadiazine
Wound Healing
collagen type 1
collagen type 3
elastin
macrogol
molecular scaffold
nanofiber
polycaprolactone
sulfadiazine silver
airbrushing method
animal experiment
animal model
animal tissue
Article
biocompatibility
burn
cell proliferation
cell structure
controlled study
dental follicle stem cell
drug efficacy
Escherichia coli
histopathology
human
human cell
in vitro study
investigative procedures
nanoencapsulation
nanofabrication
nonhuman
regenerative ability
skin fibroblast
stem cell
tissue differentiation
tooth germ
upregulation
wound healing
zone of inhibition
animal
bandage
Leporidae
M3 - Article
PY - 2022
ST - Silver sulfadiazine loaded core-shell airbrushed nanofibers for burn wound
healing application
TI - Silver sulfadiazine loaded core-shell airbrushed nanofibers for burn wound
healing application
85121351930&doi=10.1016%2fj.ijpharm.2021.121358&partnerID=40&md5=7c4ddaeaa0438e4415
545e74691fc5bc
VL - 613
ID - 5117
ER -
TY - JOUR
AB - Background: Acacetin is a di-hydroxy and mono-methoxy flavone present in
various plants, including black locust, Damiana, Silver birch. Literature
information revealed that acacetin exhibits an array of pharmacological potential
including chemopreventive and cytotoxic properties in cancer cell lines, prevents
ischemia/reperfusion/myocardial infarction-induced cardiac injury,
lipopolysaccharide (LPS), 1-methyl-4-phenyl pyridinium ion (MPP+) and 1-methyl-4-
phenyl-1,2,3,6-tetrahydropyridine hydrochloride (MPTP)-induced neuroinflammation,
LPS and sepsis-induced lung injury, rheumatoid and collagen-induced arthritis,
inhibit the microbial growth, obesity, viral-mediated infections as well as hepatic
protection. Purpose: This review highlights the therapeutic potential of acacetin,
with updated and comprehensive information on the biological sources, chemistry,
and pharmacological properties along with the possible mechanism of action, safety
aspects, and future research opportunities. Study design: The information was
retrieved from various search engines, including Pubmed, SciFinder, Science direct,
Inxight:drugs, Google scholar, and Meta cyc. Result: The first section of this
review focuses on the detailed biological source of acacetin, chromatographic
techniques used for isolation, chemical characteristics, the method for the
synthesis of acacetin, and the available natural and synthetic derivatives.
Subsequently, the pharmacological activities, including anti-cancer, anti-
inflammatory, anti-viral, anti-microbial, anti-obesity, have been discussed. The
pharmacokinetics data and toxicity profile of acacetin are also discussed.
Conclusion: Acacetin is a potent molecule reported for its strong anti-inflammatory
and anti-cancer activity, however further scientific evidence is essential to
validate its potency in disease models associated with inflammation and cancer.
There is limited information available for toxicity profiling of acacetin;
therefore, further studies would aid in establishing this natural flavone as a
potent candidate for research studies at clinical setup. © 2020 Elsevier Ltd
AU - Singh, S.
AU - Gupta, P.
AU - Meena, A.
AU - Luqman, S.
C7 - 111708
DB - Scopus
DO - 10.1016/j.fct.2020.111708
KW - Acacetin
Anti-cancer
Antioxidants
Flavone
Natural product
Pharmacological activity
Animals
Antimetabolites
Flavones
Humans
Inflammation
Metabolic Diseases
Neoplasms
acacetin
antimetabolite
flavone derivative
acute lung injury
antiaging activity
antileishmanial activity
antimalarial activity
antinociception
antiobesity activity
antipyretic activity
antiviral activity
Article
biological activity
chemistry
chromatography
clinical research
drug cytotoxicity
drug efficacy
drug isolation
drug safety
drug structure
drug synthesis
evidence based practice
heart protection
human
hypouricemia
infection
inflammation
metabolic disorder
neuroprotection
nonhuman
vasodilatation
animal
neoplasm
M3 - Article
PY - 2020
ST - Acacetin, a flavone with diverse therapeutic potential in cancer,
inflammation, infections and other metabolic disorders
TI - Acacetin, a flavone with diverse therapeutic potential in cancer,
inflammation, infections and other metabolic disorders
85090599665&doi=10.1016%2fj.fct.2020.111708&partnerID=40&md5=1b47c067137a51c4caa38c
9fd091f273
VL - 145
ID - 5267
ER -
TY - JOUR
AB - The present study evaluates in vitro cytotoxic effects and the mode of
interaction of biologically synthesized Ag and Au nanoparticles (NPs) using
Brassica oleracea L. var. capitata f. rubra (BOL) against HT-1080 cancer cells and
bacterial cells as well as their wound healing efficacy using a mouse model. UV-
visible spectroscopy, scanning electron microscopy, high-resolution transmission
electron microscopy, and energy-dispersive X-ray analysis have ascertained the
formation of nano-sized Ag and Au particles. Fourier transform infrared analysis
has confirmed that polyphenol and amide groups in BOL act as capping as well as
reducing agents. The free radical scavenging activity under in vitro conditions is
found to be higher for the Ag NPs when compared to the Au NPs. Acridine orange-
ethidium bromide dual staining and comet assay have indicated that the cytotoxic
effects are mediated through nuclear morphological changes and DNA damage. The
intracellular localization of Ag and Au NPs in HT-1080 cells and their subsequent
effect on apoptosis and necrosis were analyzed by flow cytometry while the mode of
interaction was established by scanning electron microscopy under field emission
mode and by bio-transmission electron microscopy. These methods of analysis clearly
revealed that the Ag and Au NPs have easily entered and accumulated into the
cytosol and nucleus, resulting in activation of inflammatory and apoptosis
pathways, which in turn cause damage in DNA. Further, mRNA and protein expression
of caspase- 3 and caspase- 7, TNF-alpha, and NF-kappa B have provided sufficient
clues for induction of intrinsic and extrinsic apoptosis and inflammatory pathways
in Ag NP- and Au NP-treated cells. Evaluation of wound healing properties of Ag and
Au NPs using a mouse model indicates rapid healing of wounds. In addition, no clear
toxic effects and no nuclear abnormalities in peripheral blood cells are observed.
Ag NPs appear to be a better anticancer therapeutic agent than Au NPs. Nonetheless,
both Ag NPs and Au NPs show potential for promoting topical wound healing without
any toxic effects.
AN - WOS:000405804100008
AU - Sivakumar, A. S.
AU - Krishnaraj, C.
AU - Sheet, S.
AU - Rampa, D. R.
AU - Kang, D. R.
AU - Belal, S. A.
AU - Kumar, A.
AU - Hwang, I. H.
AU - Yun, S. I.
AU - Lee, Y. S.
AU - Shim, K. S.
DA - AUG
DO - 10.1007/s11626-017-0150-5
IS - 7
PY - 2017
SN - 1071-2690
1543-706X
SP - 632-645
ST - Interaction of silver and gold nanoparticles in mammalian cancer: as real
topical bullet for wound healing- A comparative study
T2 - IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
TI - Interaction of silver and gold nanoparticles in mammalian cancer: as real
topical bullet for wound healing- A comparative study
VL - 53
ID - 6366
ER -
TY - JOUR
AB - The present study evaluates in vitro cytotoxic effects and the mode of
interaction of biologically synthesized Ag and Au nanoparticles (NPs) using
Brassica oleracea L. var. capitata f. rubra (BOL) against HT-1080 cancer cells and
bacterial cells as well as their wound healing efficacy using a mouse model. UV–
visible spectroscopy, scanning electron microscopy, high-resolution transmission
electron microscopy, and energy-dispersive X-ray analysis have ascertained the
formation of nano-sized Ag and Au particles. Fourier transform infrared analysis
has confirmed that polyphenol and amide groups in BOL act as capping as well as
reducing agents. The free radical scavenging activity under in vitro conditions is
found to be higher for the Ag NPs when compared to the Au NPs. Acridine orange–
ethidium bromide dual staining and comet assay have indicated that the cytotoxic
effects are mediated through nuclear morphological changes and DNA damage. The
intracellular localization of Ag and Au NPs in HT-1080 cells and their subsequent
effect on apoptosis and necrosis were analyzed by flow cytometry while the mode of
interaction was established by scanning electron microscopy under field emission
mode and by bio-transmission electron microscopy. These methods of analysis clearly
revealed that the Ag and Au NPs have easily entered and accumulated into the
cytosol and nucleus, resulting in activation of inflammatory and apoptosis
pathways, which in turn cause damage in DNA. Further, mRNA and protein expression
of caspase-3 and caspase-7, TNF-α, and NF-κB have provided sufficient clues for
induction of intrinsic and extrinsic apoptosis and inflammatory pathways in Ag NP-
and Au NP-treated cells. Evaluation of wound healing properties of Ag and Au NPs
using a mouse model indicates rapid healing of wounds. In addition, no clear toxic
effects and no nuclear abnormalities in peripheral blood cells are observed. Ag NPs
appear to be a better anticancer therapeutic agent than Au NPs. Nonetheless, both
Ag NPs and Au NPs show potential for promoting topical wound healing without any
toxic effects. © 2017, The Society for In Vitro Biology.
AU - Sivakumar, A. S.
AU - Krishnaraj, C.
AU - Sheet, S.
AU - Rampa, D. R.
AU - Kang, D. R.
AU - Belal, S. A.
AU - Kumar, A.
AU - Hwang, I. H.
AU - Yun, S. I.
AU - Lee, Y. S.
AU - Shim, K. S.
DB - Scopus
DO - 10.1007/s11626-017-0150-5
IS - 7
KW - Apoptosis
Cancer
Gold
Inflammation
Nanoparticles
Peripheral blood
Silver
Wound healing
Animals
Caspase 3
Cell Line, Tumor
Cell Membrane
Cell Shape
Comet Assay
Male
Metal Nanoparticles
Mice, Inbred ICR
Micronucleus Tests
Necrosis
Neoplasms
Skin
Staining and Labeling
Wound Healing
caspase 3
gold
metal nanoparticle
silver
animal
apoptosis
cell membrane
cell shape
chemistry
comet assay
comparative study
drug effects
inflammation
male
metabolism
micronucleus test
necrosis
neoplasm
pathology
skin
staining
tumor cell line
ultrastructure
wound healing
M3 - Article
PY - 2017
SP - 632-645
ST - Interaction of silver and gold nanoparticles in mammalian cancer: as real
topical bullet for wound healing— A comparative study
T2 - In Vitro Cellular and Developmental Biology - Animal
TI - Interaction of silver and gold nanoparticles in mammalian cancer: as real
topical bullet for wound healing— A comparative study
85018262549&doi=10.1007%2fs11626-017-0150-
5&partnerID=40&md5=a17e52da055d661245481f1250d53528
VL - 53
ID - 5545
ER -
TY - JOUR
AB - Green silver nanoparticles have received much interest over the years because
they are cheap, good for the environment, and easy to use. Present study, first
report to synthesized silver nanoparticles from the leaf extract of Andrographis
macrobotrys, which reduces AgNO3 into Ag through the presence of phytochemicals.
The nanoparticles were examined using (UV, spec, FTIR, XRD, TEM and EDAX. The dark
brown colour of the A. macrobotrys colloidal showed maximum absorbance at 450 nm.
The TEM images displayed synthesised nanoparticles size were revealed between 20
and 50 nm. The antibacterial activity of Ag-NPs tested show a maximum zone of
inhibition of 19 mm for Escherichia coli and Staphylococcus aureus 17 mm for at 125
µg/mL. Green synthesized AgNPs were assessed for antioxidant activity inhibition
rate (DPPH 58.23 % and ABTS 68.87 %). Further, the anticancer activity of AgNPs
exhibited 68.15 % at 100 µg/mL concentration against A549 lung cancer cells.
Additionally, in vitro models using the human red blood cells (HRBC) membrane
stabilisation method (MSM) were used to assess the anti-inflammatory effects of
AgNPs of A. macrobotrys and its shown to have a MSM of 76.6 % at a dosage of 250
µg/mL. A. macrobotrys derived AgNPs possess multi potential activity was used in
future pharmaceutical applications. © 2023 Elsevier B.V.
AU - Sivakumar, S.
AU - Subban, M.
AU - Chinnasamy, R.
AU - Chinnaperumal, K.
AU - Nakouti, I.
AU - El-Sheikh, M. A.
AU - Shaik, J. P.
C7 - 110787
DB - Scopus
DO - 10.1016/j.inoche.2023.110787
KW - AgNPs
Andrographis macrobotrys
Antibacterial
Antioxidants
Lung cancer cells (A549)
M3 - Article
PY - 2023
ST - Green synthesized silver nanoparticles using Andrographis macrobotrys Nees
leaf extract and its potential to antibacterial, antioxidant, anti-inflammatory and
lung cancer cells cytotoxicity effects
T2 - Inorganic Chemistry Communications
TI - Green synthesized silver nanoparticles using Andrographis macrobotrys Nees
leaf extract and its potential to antibacterial, antioxidant, anti-inflammatory and
lung cancer cells cytotoxicity effects
85159218959&doi=10.1016%2fj.inoche.2023.110787&partnerID=40&md5=2ede0775a06c7468973
b177737033b9d
VL - 153
ID - 5020
ER -
TY - JOUR
AB - The emergence of bacteria resistant to antibiotics and the resulting
infections are increasingly becoming a public health issue. Multidrug-resistant
(MDR) bacteria are responsible for infections leading to increased morbidity and
mortality in hospitals, prolonged time of hospitalization, and additional burden to
financial costs. Therefore, there is an urgent need for novel antibacterial agents
that will both treat MDR infections and outsmart the bacterial evolutionary
mechanisms, preventing further resistance development. In this study, a green
synthesis employing nontoxic lignin as both reducing and capping agents was adopted
to formulate stable and biocompatible silver-lignin nanoparticles (NPs) exhibiting
antibacterial activity. The resulting silver-lignin NPs were approximately 20 nm in
diameter and did not agglomerate after one year of storage at 4 °C. They were able
to inhibit the growth of a panel of MDR clinical isolates, including Staphylococcus
aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella pneumoniae,
and Acinetobacter baumannii, at concentrations that did not affect the viability of
a monocyte-derived THP-1 human cell line. Furthermore, the exposure of silver-
lignin NPs to the THP-1 cells led to a significant increase in the secretion of the
anti-inflammatory cytokine IL-10, demonstrating the potential of these particles to
act as an antimicrobial and anti-inflammatory agent simultaneously. P. aeruginosa
genes linked with efflux, heavy metal resistance, capsular biosynthesis, and quorum
sensing were investigated for changes in gene expression upon sublethal exposure to
the silver-lignin NPs. Genes encoding for membrane proteins with an efflux function
were upregulated. However, all other genes were membrane proteins that did not
efflux metals and were downregulated. © 2021 The Authors. Published by American
Chemical Society.
AU - Slavin, Y. N.
AU - Ivanova, K.
AU - Hoyo, J.
AU - Owen, G.
AU - Haegert, A.
AU - Lin, Y. Y.
AU - Lebihan, S.
AU - Gedanken, A.
AU - Häfeli, U. O.
AU - Tzanov, T.
AU - Bach, H.
DB - Scopus
DO - 10.1021/acsami.0c16921
IS - 19
cytotoxicity
gene expression
membrane model
microscopy
Agents
Bacteria
Exposure
Genes
Lignins
Proteins
Resistance
Silver
Humans
Inflammation
Lignin
Metal Nanoparticles
THP-1 Cells
Biochemistry
Biocompatibility
Cell culture
Gene expression
Green Synthesis
Heavy metals
Hospitals
Nanoparticles
antiinfective agent
lignin
metal nanoparticle
silver
Evolutionary mechanisms
Heavy metal resistance
Public health issues
bacterium
chemistry
drug effect
human
inflammation
THP-1 cell line
M3 - Article
PY - 2021
SP - 22098-22109
ST - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
TI - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
85106393520&doi=10.1021%2facsami.0c16921&partnerID=40&md5=4345078debc57484ef201dc8d
34b03fa
VL - 13
ID - 5234
ER -
TY - JOUR
AB - The emergence of bacteria resistant to antibiotics and the resulting
infections are increasingly becoming a public health issue. Multidrug-resistant
(MDR) bacteria are responsible for infections leading to increased morbidity and
mortality in hospitals, prolonged time of hospitalization, and additional burden to
financial costs. Therefore, there is an urgent need for novel antibacterial agents
that will both treat MDR infections and outsmart the bacterial evolutionary
mechanisms, preventing further resistance development. In this study, a green
synthesis employing nontoxic lignin as both reducing and capping agents was adopted
to formulate stable and biocompatible silver-lignin nanoparticles (NPs) exhibiting
antibacterial activity. The resulting silver-lignin NPs were approximately 20 nm in
diameter and did not agglomerate after one year of storage at 4 degrees C. They
were able to inhibit the growth of a panel of MDR clinical isolates, including
Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa,
Klebsiella pneumoniae, and Acinetobacter baumannii, at concentrations that did not
affect the viability of a monocyte-derived THP-1 human cell line. Furthermore, the
exposure of silver-lignin NPs to the THP-1 cells led to a significant increase in
the secretion of the anti-inflammatory cytokine IL-10, demonstrating the potential
of these particles to act as an antimicrobial and anti-inflammatory agent
simultaneously. P. aeruginosa genes linked with efflux, heavy metal resistance,
capsular biosynthesis, and quorum sensing were investigated for changes in gene
expression upon sublethal exposure to the silver-lignin NPs. Genes encoding for
membrane proteins with an efflux function were upregulated. However, all other
genes were membrane proteins that did not efflux metals and were downregulated.
AN - WOS:000655027500002
AU - Slavin, Y. N.
AU - Ivanova, K.
AU - Hoyo, J.
AU - Owen, G.
AU - Haegert, A.
AU - Lin, Y. Y.
AU - LeBihan, S.
AU - Gedanken, A.
AU - Hafeli, U. O.
AU - Tzanov, T.
AU - Bach, H.
C6 - MAY 2021
DA - MAY 19
DO - 10.1021/acsami.0c16921
IS - 19
PY - 2021
SN - 1944-8244
1944-8252
SP - 22098-22109
ST - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
TI - Novel Lignin-Capped Silver Nanoparticles against Multidrug-Resistant Bacteria
VL - 13
ID - 5983
ER -
TY - JOUR
AB - Background: Pathogenic intracellular mycobacteria are challenging to treat
because of the waxy and complex cell wall characterizing the genus. Niosomes are
vesicles with biomimetic cell membrane composition, which allow them to efficiently
bind to the eukaryotic cells and deliver their cargo into the cytoplasm. The
objective of this study was to develop a new platform based on niosomes loaded with
antimicrobial agents to target intracellular mycobacteria. Nanoniosomes were
fabricated and loaded with antibiotics and lignin–silver nanoparticles. The
efficacy of these nanon-iosomes was tested against the intracellular pathogen
Mycobacterium abscessus used as a model of infection of human-derived macrophages
(THP-1). The cytotoxicity and the immunological response of the agents were tested
on THP-1 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
(MTT) assay and the secretion of pro-and anti-inflammatory cytokines, respectively.
Results: M. abscessus was susceptible to the nanoniosomes in infected THP-1
macrophages, suggesting that the nanoniosomes were internalized due to their fusion
to the macrophage cellular membrane. Moreover, nanoniosomes showed no upregulation
of pro-inflammatory cytokines when exposed to THP-1 macrophages. Conclusions:
Nanoniosomes improved drug efficacy while decreasing toxicity and should be
considered for further testing in the treatment of intracellular pathogenic
mycobacteria or as a new platform for precise intracellular delivery of drugs. ©
AU - Slavin, Y. N.
AU - Ivanova, K.
AU - Tang, W. L.
AU - Tzanov, T.
AU - Li, S. D.
AU - Bach, H.
C7 - 1984
DB - Scopus
DO - 10.3390/nano11081984
IS - 8
KW - Antibiotic
Lignin
Macrophage
Mycobacteria
Nanoparticle
Niosome
M3 - Article
PY - 2021
ST - Targeting intracellular mycobacteria using nanosized niosomes loaded with
antibacterial agents
T2 - Nanomaterials
TI - Targeting intracellular mycobacteria using nanosized niosomes loaded with
antibacterial agents
85111610889&doi=10.3390%2fnano11081984&partnerID=40&md5=96929d9e5da989e079bda3e4c86
40be1
VL - 11
ID - 5161
ER -
TY - JOUR
AB - Purpose: To test the antimicrobial properties, surface topography, reaction
of surrounding tissue (biocompatibility), and osseointegration of ultrathin implant
surfaces containing polysiloxane and nanoscaled silver particles. Materials and
Methods: Implants with polysiloxane coating and nanoscaled silver particles
(Ag/SiOxCy; HyProtect, Bio-Gate) were compared with implants with polysiloxane
coating alone and with noncoated (grit-blasted and acid-etched) implants. A total
of 72 implants were inserted into the calvaria of eight domestic pigs (nine
implants each, three of each type). After 3 months, histologic sections were
evaluated for inflammatory cell infiltration and bone implant contact. Results:
Roughness parameters did not differ between all three implant types. The Ag/SiOxCy
coating exhibited a good antimicrobial effect in vitro and no sign of inflammatory
cell infiltration in vivo. The noncoated implants demonstrated 10.85% and 14.48%
more bone contact than the polysiloxane-coated implants (P =.003) and the
Ag/SiOxCy-coated implants (P ≤.001), respectively. Osseointegration was not
significantly different between the Ag/SiOxCy-coated and polysiloxane-coated
implants (P =.72). Conclusion: The osseointegration capability of the Ag/SiOxCy-
coated implants was equal to that of the polysiloxane-coated implants but less than
that of the grit-blasted and acid-etched implants. Because of the biocompatibility
of the polysiloxane coating, further studies should be conducted in load-bearing
models and in the oral cavity to investigate the antimicrobial effect of the
embedded silver clusters. © 2017 by Quintessence Publishing Co Inc.
AU - Smeets, R.
AU - Precht, C.
AU - Hahn, M.
AU - Jung, O.
AU - Hartjen, P.
AU - Heiland, M.
AU - Gröbe, A.
AU - Holthaus, M. G.
AU - Hanken, H.
DB - Scopus
DO - 10.11607/jomi.5533
IS - 6
KW - Implant coating
Implant surface
In vivo model
Polysiloxane coating
Silver coating
Animals
Dental Implantation, Endosseous
Dental Implants
Materials Testing
Osseointegration
Siloxanes
Silver
Surface Properties
Sus scrofa
Swine
Titanium
Weight-Bearing
siloxane
silver
titanium
animal
materials testing
osseointegration
physiology
pig
surface property
tooth implant
tooth implantation
weight bearing
M3 - Article
PY - 2017
SP - 1338-1345
ST - Biocompatibility and osseointegration of titanium implants with a silver-
doped polysiloxane coating: An in vivo pig model
T2 - International Journal of Oral and Maxillofacial Implants
TI - Biocompatibility and osseointegration of titanium implants with a silver-
doped polysiloxane coating: An in vivo pig model
85034611637&doi=10.11607%2fjomi.5533&partnerID=40&md5=cb149c21342b47b74bb61a4e81be4
f80
VL - 32
ID - 5507
ER -
TY - JOUR
AB - Purpose: To test the antimicrobial properties, surface topography, reaction
of surrounding tissue (biocompatibility), and osseointegration of ultrathin implant
surfaces containing polysiloxane and nanoscaled silver particles. Materials and
Methods: Implants with polysiloxane coating and nanoscaled silver particles
(Ag/SiOxCy; HyProtect, Bio-Gate) were compared with implants with polysiloxane
coating alone and with noncoated (grit-blasted and acid-etched) implants. A total
of 72 implants were inserted into the calvaria of eight domestic pigs (nine
implants each, three of each type). After 3 months, histologic sections were
evaluated for inflammatory cell infiltration and bone implant contact. Results:
Roughness parameters did not differ between all three implant types. The Ag/SiOxCy
coating exhibited a good antimicrobial effect in vitro and no sign of inflammatory
cell infiltration in vivo. The noncoated implants demonstrated 10.85% and 14.48%
more bone contact than the polysiloxane-coated implants (P =.003) and the
Ag/SiOxCy-coated implants (P =.001), respectively. Osseointegration was not
significantly different between the Ag/SiOxCy-coated and polysiloxane-coated
implants (P =.72). Conclusion: The osseointegration capability of the Ag/SiOxCy-
coated implants was equal to that of the polysiloxane-coated implants but less than
that of the grit-blasted and acid-etched implants. Because of the biocompatibility
of the polysiloxane coating, further studies should be conducted in load-bearing
models and in the oral cavity to investigate the antimicrobial effect of the
embedded silver clusters.
AN - WOS:000417113300018
AU - Smeets, R.
AU - Precht, C.
AU - Hahn, M.
AU - Jung, O.
AU - Hartjen, P.
AU - Heiland, M.
AU - Grobe, A.
AU - Holthaus, M. G.
AU - Hanken, H.
DA - NOV-DEC
DO - 10.11607/jomi.5533
IS - 6
PY - 2017
SN - 0882-2786
1942-4434
SP - 1338-1345
ST - Biocompatibility and Osseointegration of Titanium Implants with a Silver-
Doped Polysiloxane Coating: An In Vivo Pig Model
T2 - INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS
TI - Biocompatibility and Osseointegration of Titanium Implants with a Silver-
Doped Polysiloxane Coating: An In Vivo Pig Model
VL - 32
ID - 6186
ER -
TY - JOUR
AB - The unique physical and chemical properties of nanomaterials have led to
their increased use in many industrial applications, including as a paint additive.
For example, titanium dioxide (TiO2) engineered nanoparticles (ENPs) have well-
established anti-UV, self-cleaning, and air purification effects. Silver (Ag) ENPs
are renowned for their anti-microbial capabilities and silicon dioxide (SiO2) ENPs
are used as fire retardants and anti-scratch coatings. In this study, the toxic
effects and biodistribution of three pristine ENPs (TiO2, Ag, and SiO2), three aged
paints containing ENPs (TiO2, Ag, and SiO2) along with control paints without ENPs
were compared. BALB/c mice were oropharyngeally aspirated with ENPs or paint
particles (20 μg/aspiration) once a week for 5 weeks and sacrificed either 2 or 28
days post final aspiration treatment. A bronchoalveolar lavage was performed and
systemic blood toxicity was evaluated to ascertain cell counts, induction of
inflammatory cytokines, and key blood parameters. In addition, the lung, liver,
kidney, spleen, and heart were harvested and metal concentrations were determined.
Exposure to pristine ENPs caused subtle effects in the lungs and negligible
alterations in the blood. The most pronounced toxic effects were observed after Ag
ENPs exposure; an increased neutrophil count and a twofold increase in pro-
inflammatory cytokine secretion (keratinocyte chemoattractant (KC) and interleukin-
1ß (IL-1ß)) were identified. The paint containing TiO2 ENPs did not modify
macrophage and neutrophil counts, but mildly induced KC and IL-1ß. The paints
containing Ag or SiO2 did not show significant toxicity. Biodistribution
experiments showed distribution of Ag and Si outside the lung after aspiration to
respectively pristine Ag or SiO2 ENPs. In conclusion, we demonstrated that even
though direct exposure to ENPs induced some toxic effects, once they were embedded
in a complex paint matrix little to no adverse toxicological effects were
identified. © The Author 2014. Published by Oxford University Press on behalf of
AU - Smulders, S.
AU - Luyts, K.
AU - Brabants, G.
AU - Van Landuyt, K.
AU - Kirschhock, C.
AU - Smolders, E.
AU - Golanski, L.
AU - Vanoirbeek, J.
AU - Hoet, P. H. M.
DB - Scopus
DO - 10.1093/toxsci/kfu112
IS - 1
KW - In vivo
Inhalation toxicology
Mice
Nanotoxicology
Occupational health
Animals
Blood Cell Count
Cytokines
Inhalation Exposure
Male
Metal Nanoparticles
Mice, Inbred BALB C
Microscopy, Electron
Nanoparticles
Paint
Silicon Dioxide
Silver
Surface Properties
Tissue Distribution
Titanium
Mus
chemoattractant
interleukin 1beta
keratinocyte chemoattractant
nanoparticle
paint
silicon dioxide nanoparticle
silver nanoparticle
unclassified drug
cytokine
metal nanoparticle
silicon dioxide
silver
titanium
titanium dioxide
animal experiment
Article
aspiration
blood toxicity
cell count
controlled study
cytokine release
cytotoxicity
lung lavage
lung toxicity
mouse
nanotoxicology
neutrophil count
nonhuman
pneumonia
protein analysis
animal
Bagg albino mouse
biosynthesis
blood cell count
chemistry
electron microscopy
exposure
male
metabolism
surface property
tissue distribution
M3 - Article
PY - 2014
SP - 132-140
ST - Toxicity of nanoparticles embedded in paints compared with pristine
nanoparticles in mice
TI - Toxicity of nanoparticles embedded in paints compared with pristine
nanoparticles in mice
84913613623&doi=10.1093%2ftoxsci
%2fkfu112&partnerID=40&md5=c4cb63344345db50b5333dfe29c7cbe1
VL - 141
ID - 5604
ER -
TY - JOUR
AB - Deep burn injuries are complicated traumas accompanying severe oxidative
stress and impairment of inherent cellular defense mechanisms against external
bacteria. Accordingly, it is required to suppress oxidative stress and support the
recovery of intrinsic defense systems in burn wound lesions, but limited topical
treatments are clinically available. Herein, antioxidative and anti-inflammatory
2H-WS2 nanosheets are developed as a biocompatible, topical antipyrotic for the
treatment of deep burn wounds. The 2H-WS2 nanosheets functionalized with a block
copolymer can effectively suppress extrinsic apoptosis, lipid peroxidation, and
inflammation in human keratinocytes by scavenging intracellular reactive oxygen
species (ROS) and reactive nitrogen species (RNS). Moreover, the 2H-WS2 nanosheets
markedly stimulate the up-regulation of innate antioxidant enzymes and
antimicrobial peptides in the skin cells, enhancing their intrinsic cellular
defense systems. The combined effects of this 2H-WS2 antipyrotic enables a higher
efficacy in the deep burn wounds of mice, accelerating re-epithelialization and
satisfactory wound healing as compared with commercial silver sulfadiazine (SSD).
The therapeutic mechanism of the 2H-WS2 nanosheets for deep burn wounds is proposed
herein and found to be distinctly different from that of SSD. The nanotherapeutics
of multifunctional 2H-WS2 nanosheets show potential for use in the treatment of
other oxidative stress-induced and inflammatory diseases. © 2022 Elsevier Ltd
AU - So, Y.
AU - Yim, D.
AU - Son, W.
AU - Lee, H.
AU - Lee, S.
AU - Choi, C.
AU - Yang, C. S.
AU - Kim, J. H.
C7 - 101591
DB - Scopus
DO - 10.1016/j.apmt.2022.101591
KW - Anti-apoptosis
Anti-inflammation
Burn wound healing
Oxidative stress suppression
WS2 nanosheet antipyrotic
Antioxidants
Biocompatibility
Block copolymers
Cell death
Diseases
Mammals
Network security
Oxidative stress
Pathology
Tungsten compounds
Burn injury
Cellulars
Deep burn
Defence systems
Wound healing
WS2 nanosheet antipyrotic
Nanosheets
M3 - Article
PY - 2022
ST - Deciphering the therapeutic mechanism of topical WS2 nanosheets for the
effective therapy of burn injuries
T2 - Applied Materials Today
TI - Deciphering the therapeutic mechanism of topical WS2 nanosheets for the
effective therapy of burn injuries
85134852699&doi=10.1016%2fj.apmt.2022.101591&partnerID=40&md5=97309a945a66a8245513c
84b7bd8621a
VL - 29
ID - 4958
ER -
TY - JOUR
AB - Background: Wound healing is a complicated process involving the
proliferation of the epithelial cells, deposition of granulation tissue as well as
recruitment of inflammatory cells. It also is a hot topic of research for trauma,
orthopedics and general surgery studies. There are many forms of cells involved in
this process. This study aimed to design a tissue-engineered wound dressing
consisting of chitosan fibers containing silver ion bioactive nanoparticles for
wound healing. Methods: The present study is an experimental study that was
conducted in the research laboratory of the Department of Biology of Mohaghegh
Ardabili University from April to November 2019. All experiments of this study have
been performed under the ethical guideline of Helsinki and in accordance with the
Ethics Committee of the Mohaghegh Ardabili University of Ardabil (Iran). The wound
dressing of nanofibers was prepared by the sol-gel method. Cytotoxicity was
assessed by MTT assay. Then the antimicrobial properties of nanofibers were
determined by the disk diffusion method. SEM and AFM images were obtained from
nanofibers. Finally, nanofibers were analyzed by the FTRI method. Results: Results
of the prepared tissue-engineered wound dressing consisting of chitosan fibers
containing silver ion-doped bioactive nanoparticles showed that cytotoxicity was at
an appropriate level. The nanofibers prepared with 2% silver nanoparticles produced
a 10 mm inhibition zone against Staphylococcus aureus and a 9 mm inhibition zone
against Escherichia coli. Therefore, the best percentage of scaffolds in the
present study was 2%. Also, results of the SEM micrographs and AFM image analysis
of the scaffolds showed that the nanofibers had good roughness and a proper
structure for cell seeding and attachments. Besides that, FTIR analysis also showed
that the prepared nanofibers had standard bonds. Conclusion: Chitosan-Silver
nanoparticles scaffold have antimicrobial activity on Gram-negative and positive
bacteria. The results of the toxicity test also showed that it did not have much
toxicity on the cultured cells. Therefore, it can be considered for therapeutic
applications, such as wound dressing. © 2021 Tehran University of Medical Sciences.
AU - Sohi, M. M.
AU - Asadi, A.
AU - Milan, P. B.
AU - Sharifi, E.
AU - Abdolmaleki, A.
DB - Scopus
IS - 4
KW - chitosan
nanoparticles
regeneration
tissue engineering
nanofiber
silver nanoparticle
Article
cell adhesion
cytotoxicity
disk diffusion
Escherichia coli
human
human tissue
MTT assay
sol-gel
tumor seeding
wound healing
zone of inhibition
M3 - Article
PY - 2021
SP - 290-298
ST - Preparation of tissue-engineered wound dressing consisting of chitosan fibers
containing silver ion-doped bioactive nanoparticles for wound healing
T2 - Tehran University Medical Journal
TI - Preparation of tissue-engineered wound dressing consisting of chitosan fibers
containing silver ion-doped bioactive nanoparticles for wound healing
85110447721&partnerID=40&md5=23b329e78dc778d13ead63ac7202848b
VL - 79
ID - 5187
ER -
TY - JOUR
AB - The body's normal immune response against any invading pathogen that causes
infection in the body results in inflammation. The sudden transformation in
inflammation leads to the rise of inflammatory diseases such as chronic
inflammatory bowel disease, autoimmune disorders, and colorectal cancer (different
types of cancer develop at the site of chronic infection and inflammation).
Inflammation results in two ways: short-term inflammation i.e., non-specific,
involves the action of various immune cells; the other results in long-term
reactions lasting for months or years. It is specific and causes angiogenesis,
fibrosis, tissue destruction, and cancer progression at the site of inflammation.
Cancer progression relies on the interaction between the host microenvironment and
tumor cells along with the inflammatory responses, fibroblast, and vascular cells.
The two pathways that have been identified connecting inflammation and cancer are
the extrinsic and intrinsic pathways. Both have their own specific role in linking
inflammation to cancer, involving various transcription factors such as Nuclear
factor kappa B, Activator of transcription, Single transducer, and Hypoxia-
inducible factor, which in turn regulates the inflammatory responses via Soluble
mediators cytokines (such as Interleukin-6, Hematopoietin-1/Erythropoietin, and
tumor necrosis factor), chemokines (such as Cyclooxygenase-2, C-X-C Motif
chemokines ligand-8, and IL-8), inflammatory cells, cellular components (such as
suppressor cells derived from myeloid, tumor-associated macrophage, and
acidophils), and promotes tumorigenesis. The treatment of these chronic
inflammatory diseases is challenging and needs early detection and diagnosis.
Nanotechnology is a booming field nowadays for its rapid action and easy
penetration inside the infected destined cells. Nanoparticles are widely classified
into different categories based on their different factors and properties such as
size, shape, cytotoxicity, and others. Nanoparticles emerged as excellent with
highly progressive medical inventions to cure diseases such as cancer, inflammatory
diseases, and others. Nanoparticles have shown higher binding capacity with the
biomolecules in inflammation reduction and lowers the oxidative stress inside
tissue/cells. In this review, we have overall discussed inflammatory pathways that
link inflammation to cancer, major inflammatory diseases, and the potent action of
nanoparticles in chronic inflammation-related diseases.
AN - WOS:001004712300001
AU - Sohrab, S. S.
AU - Raj, R.
AU - Nagar, A.
AU - Hawthorne, S.
AU - Paiva-Santos, A. C.
AU - Kamal, M. A.
AU - El-Daly, M. M.
AU - Azhar, E. I.
AU - Sharma, A.
C7 - 4413
DA - MAY 29
DO - 10.3390/molecules28114413
IS - 11
PY - 2023
SN - 1420-3049
ST - Chronic Inflammation's Transformation to Cancer: A Nanotherapeutic Paradigm
T2 - MOLECULES
TI - Chronic Inflammation's Transformation to Cancer: A Nanotherapeutic Paradigm
VL - 28
ID - 6516
ER -
TY - JOUR
AB - Moringa oleifera and Azadirachta indica (Neem) are trees with nutritional,
pharmacological values, and various preparations from almost all parts of the plant
especially from leaves are used in folk medicine for many infirmity treatments.
This study aimed to investigate the polyphenolic burden, in vitro antioxidant, and
anti-inflammatory properties of M. oleifera, Neem leaves crude aqueous extracts and
synthesized Ag-NPs. Moringa oleifera and Neem leaves were extracted using de-
ionized water. The extracts were screened for phenolic compounds by HPLC analysis.
Ag-NPs were obtained by a modified method, in the presence of NaBH4 at its half
usual concentration, and they were characterized by UV-VIS, Zeta potential &
particle size, and TEM respectively. The antioxidant activity was determined by
DPPH, ABTS, and FRAP assays, and the anti-inflammatory activity was determined
against macrophage cell line RAW 264.7 by Nitric Oxide assay. The results indicated
that synthesized Ag-NPs from Moringa and Neem show enhanced antioxidant with (75.47
+/- 0.16%) and (65.97 +/- 0.17%) at 400 mu g/mL according to DPPH assay, ABTS assay
with (82.93 +/- 0.63%) and (78.76 +/- 0.34%) at 200 mu g/mL, and FRAP assay with
(113.67 +/- 0.68 mu M) and (105.31 +/- 0.33 mu M) at 400 mu g/mL, respectively.
After the LPS treatment, the anti-inflammatory potential of different tested
samples showed (67.95 +/- 0.85%) and (58.29 +/- 0.90%) inhibition at 100 mu g/mL
for Ag-NPs from Moringa and Neem, compared to Moringa and Neem crude aqueous
extracts with (30.60 +/- 0.43%) and (26.25 +/- 1.28%) at 100 mu g/mL respectively.
Synthesized Ag-NPs and crude aqueous extracts from Moringa and Neem may be
effectively used to possess important antioxidant and anti-inflammatory properties.
AN - WOS:000599707800009
AU - Solaiman, M. A.
AU - Ali, M. A.
AU - Abdel-Moein, N. M.
AU - Mahmoud, E. A.
C7 - 101832
DA - OCT
DO - 10.1016/j.bcab.2020.101832
PY - 2020
SN - 1878-8181
ST - Synthesis of Ag-NPs developed by green-chemically method and evaluation of
antioxidant activities and anti-inflammatory of synthesized nanoparticles against
LPS-induced NO in RAW 264.7 macrophages
T2 - BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
TI - Synthesis of Ag-NPs developed by green-chemically method and evaluation of
antioxidant activities and anti-inflammatory of synthesized nanoparticles against
LPS-induced NO in RAW 264.7 macrophages
VL - 29
ID - 6100
ER -
TY - JOUR
AB - Introduction The success of endodontic surgery depends on the
histocompatibility of the root-end filling material. Applications of nanotechnology
improve their performance. Aim of the work Aim of the work was to compare the
effect of a mineral trioxide aggregate (MTA) and a bioceramic nanoparticulate
bioaggregate (BNB) on the histological structure of draining axillary lymph nodes
of adult male albino rats after their surgical implantation into the skin.
Materials and methods Forty adult male albino rats were divided into control and
experimental groups. The latter was subdivided into MTA and BNB surgically
implanted subgroups. After 7 days, the rats were sacrificed. Paraffin sections from
both the proximal part of the dorsal skin and draining axillary lymph nodes were
processed for HandE staining. Lymph node sections were further subjected to silver
reticulin, Verhoeff's Van Gieson stains as well as kappa light chains. Quantitative
assessments and statistical analysis of the results were carried out. Results There
was an increase in mononuclear inflammatory cells infiltrating the skin in the MTA
subgroup. Lymph nodes of the MTA subgroup showed a marked decrease in the
lymphocyte content of lymphatic nodules, wide lymph sinuses, multinucleate giant
cells, and many macrophages. In the BNB-treated subgroup, lymphatic nodules had
wide corona and small germinal centers. Reticular and collagen fibers were
increased in the MTA subgroup. Kappa light chains' immunoreactions were strong
positive in MTA and mild positive in BNB subgroups. A highly significant increase
in the mean area% of all fibers and kappa light chain immunoexpression of lymph
nodes of the MTA subgroup were observed. Conclusion and recommendation MTA had less
biocompatibility. BNB showed limited signs of acute inflammation. BNB is an up-to-
date alternative to the currently used root-end filling materials. The chronic
effects caused by BNB may require further study. © 2012 The Egyptian Journal of
Histology.
AU - Soliman, H. M.
AU - Anwar, R. I.
DB - Scopus
DO - 10.1097/01.EHX.0000420214.04776.64
IS - 4
KW - Bioceramic Nanoparticulate Bioaggregate
Collagen Fibers
Lymph Node
Mineral Trioxide Aggregate
Root-End Filling Materials
Skin
M3 - Article
PY - 2012
SP - 736-748
ST - Effect of surgically implanted root-end filling materials on the structure of
draining lymph nodes of male albino rats: Histological and immunohistochemical
study
TI - Effect of surgically implanted root-end filling materials on the structure of
draining lymph nodes of male albino rats: Histological and immunohistochemical
study
84878078639&doi=10.1097%2f01.EHX.0000420214.04776.64&partnerID=40&md5=730fc06ed5919
c7f3b4d36eedf32909f
VL - 35
ID - 5729
ER -
TY - JOUR
AB - Here, we prepared gold silver alloy nanoparticle (AgAuNPcs) of similar to 8.5
nm mean size via one-pot biogenic synthesis using casein as reducing as well as
capping agent and explored its potential in various biomedical applications using
curcumin (CUR) as a drug delivery agent. When we prepared the nanoconjugate with
curcumin (AgAuNPCS-CUR) of 10.5 nm mean size via chemical activation followed by
loading, it demonstrated excellent curcumin loading capacity (221.19 mg/g).
Furthermore, AgAuNPCS-CUR exhibited pH driven CUR release up to a maximum of 85% at
a pH 6.0. We also observed its significant storage stability up to 30 days at 4
degrees C. When applied in cancer cells, the nanoconjugate demonstrated higher
cytotoxicity to multiple cancer cells (MCF-7, MDAMB231, HeLa and MG 63) than free
CUR. However, such cytotoxicity was further enhanced by nearly similar to 8-10%
using the folate receptor (FR)-based targeting. Furthermore, it demonstrated
excellent antioxidant, as well as anti-inflammatory activity (NO assay) and such
activity was found higher than that of free CUR. Both AgAuNPcs and AgAuNPCS-CUR
also exhibited excellent cytocompatibility to human keratinocyte and erythrocyte
cells. Hence, from the overall findings, we concluded that our biogenically
synthesized AgAuNPCS alloy nanoparticle has, anti-inflammatory, anti-bacterial,
anti-oxidant activity as well as potential for targeted therapy (in cancer) via
controlled CUR delivery in biomedical applications. (C) 2019 Elsevier B.V. All
rights reserved.
AN - WOS:000487168000050
AU - Somu, P.
AU - Paul, S.
C7 - 111303
DA - OCT 1
DO - 10.1016/j.molliq.2019.111303
PY - 2019
SN - 0167-7322
1873-3166
ST - Protein assisted one pot controlled synthesis of monodispersed and
multifunctional colloidal silver gold alloy nanoparticles
T2 - JOURNAL OF MOLECULAR LIQUIDS
TI - Protein assisted one pot controlled synthesis of monodispersed and
multifunctional colloidal silver gold alloy nanoparticles
VL - 291
ID - 6297
ER -
TY - JOUR
AB - Here, we prepared gold-silver alloy nanoparticle (AgAuNPCS) of ~8.5 nm mean
size via one-pot biogenic synthesis using casein as reducing as well as capping
agent and explored its potential in various biomedical applications using curcumin
(CUR) as a drug delivery agent. When we prepared the nanoconjugate with curcumin
(AgAuNPCS-CUR) of 10.5 nm mean size via chemical activation followed by loading, it
demonstrated excellent curcumin loading capacity (221.19 mg/g). Furthermore,
AgAuNPCS-CUR exhibited pH driven CUR release up to a maximum of 85% at a pH 6.0. We
also observed its significant storage stability up to 30 days at 4 °C. When applied
in cancer cells, the nanoconjugate demonstrated higher cytotoxicity to multiple
cancer cells (MCF-7, MDAMB231, HeLa and MG 63) than free CUR. However, such
cytotoxicity was further enhanced by nearly ~8–10% using the folate receptor (FR)-
based targeting. Furthermore, it demonstrated excellent antioxidant, as well as
anti-inflammatory activity (NO assay) and such activity was found higher than that
of free CUR. Both AgAuNPCS and AgAuNPCS-CUR also exhibited excellent
cytocompatibility to human keratinocyte and erythrocyte cells. Hence, from the
overall findings, we concluded that our biogenically synthesized AgAuNPCS alloy
nanoparticle has, anti-inflammatory, anti-bacterial, anti-oxidant activity as well
as potential for targeted therapy (in cancer) via controlled CUR delivery in
biomedical applications. © 2019 Elsevier B.V.
AU - Somu, P.
AU - Paul, S.
C7 - 111303
DB - Scopus
DO - 10.1016/j.molliq.2019.111303
KW - Anti-cancer activity
Bioavailability
Biogenic synthesis
Curcumin delivery
Silver-gold alloy nanoparticles
Biochemistry
Biosynthesis
Cells
Chemical activation
Diseases
Gold alloys
Gold nanoparticles
Lanthanum compounds
Nanoconjugates
Nanoparticles
Alloy nanoparticle
Controlled synthesis
Curcumin
Silver alloys
M3 - Article
PY - 2019
ST - Protein assisted one pot controlled synthesis of monodispersed and
multifunctional colloidal silver-gold alloy nanoparticles
T2 - Journal of Molecular Liquids
TI - Protein assisted one pot controlled synthesis of monodispersed and
multifunctional colloidal silver-gold alloy nanoparticles
85068859380&doi=10.1016%2fj.molliq.2019.111303&partnerID=40&md5=4a524b2b85c50bc9c88
0c411bd39f43a
VL - 291
ID - 5391
ER -
TY - JOUR
AB - With the rapid development of nanotechnology, metallic (metal or metal oxide)
nanoparticles (NPs) are widely used in many fields such as cosmetics, the food and
building industries, and bio-medical instruments. Widespread applications of
metallic NP-based products increase the health risk associated with human
exposures. Studies revealed that the brain, a critical organ that consumes
substantial amounts of oxygen, is a primary target of metallic NPs once they are
absorbed into the body. Oxidative stress (OS), apoptosis, and the inflammatory
response are believed to be the main mechanisms underlying the neurotoxicity of
metallic NPs. Other studies have disclosed that antioxidant pretreatment or co-
treatment can reverse the neurotoxicity of metallic NPs by decreasing the level of
reactive oxygen species, up-regulating the activities of antioxidant enzymes,
decreasing the proportion of apoptotic cells, and suppressing the inflammatory
response. These findings suggest that the neurotoxicity of metallic NPs might
involve a cascade of events following NP-induced OS. However, additional research
is needed to determine whether NP-induced OS plays a central role in the
neurotoxicity of metallic NPs, to develop a comprehensive understanding of the
correlations among neurotoxic mechanisms and to improve the bio-safety of metallic
NP-based products.
AN - WOS:000377939300004
AU - Song, B.
AU - Zhang, Y. L.
AU - Liu, J.
AU - Feng, X. L.
AU - Zhou, T.
AU - Shao, L. Q.
C7 - 291
DA - JUN 13
DO - 10.1186/s11671-016-1508-4
PY - 2016
SN - 1931-7573
1556-276X
ST - Is Neurotoxicity of Metallic Nanoparticles the Cascades of Oxidative Stress?
T2 - NANOSCALE RESEARCH LETTERS
TI - Is Neurotoxicity of Metallic Nanoparticles the Cascades of Oxidative Stress?
VL - 11
ID - 6793
ER -
TY - JOUR
AB - Titanium dioxide nanoparticles (TiO2 NPs) possess unique characteristics and
are widely used in many fields. Numerous in vivo studies, exposing experimental
animals to these NPs through systematic administration, have suggested that TiO2
NPs can accumulate in the brain and induce brain dysfunction. Nevertheless, the
exact mechanisms underlying the neurotoxicity of TiO2 NPs remain unclear. However,
we have concluded from previous studies that these mechanisms mainly consist of
oxidative stress (OS), apoptosis, inflammatory response, genotoxicity, and direct
impairment of cell components. Meanwhile, other factors such as disturbed
distributions of trace elements, disrupted signaling pathways, dysregulated
neurotransmitters and synaptic plasticity have also been shown to contribute to
neurotoxicity of TiO2 NPs. Recently, studies on autophagy and DNA methylation have
shed some light on possible mechanisms of nanotoxicity. Therefore, we offer a new
perspective that autophagy and DNA methylation could contribute to neurotoxicity of
TiO2 NPs. Undoubtedly, more studies are needed to test this idea in the future. In
short, to fully understand the health threats posed by TiO2 NPs and to improve the
bio-safety of TiO2 NPs-based products, the neurotoxicity of TiO2 NPs must be
investigated comprehensively through studying every possible molecular mechanism.
AN - WOS:000375531100001
AU - Song, B.
AU - Zhang, Y. L.
AU - Liu, J.
AU - Feng, X. L.
AU - Zhou, T.
AU - Shao, L. Q.
DA - APR 29
DO - 10.3762/bjnano.7.57
PY - 2016
SN - 2190-4286
ST - Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on
molecular mechanisms
T2 - BEILSTEIN JOURNAL OF NANOTECHNOLOGY
TI - Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on
molecular mechanisms
VL - 7
ID - 6796
ER -
TY - JOUR
AB - Nanotechnology is a promising technology of the twenty-first century, being a
rapidly evolving field of research and industrial innovation widely applied in our
everyday life. Silver nanoparticles (AgNP) are considered the most commercialized
nanosystems worldwide, being applied in diverse sectors, from medicine to the food
industry. Considering their unique physical, chemical and biological properties,
AgNP have gained access into our daily life, with an exponential use in food
industry, leading to an increased inevitable human oral exposure. With the growing
use of AgNP, several concerns have been raised, in recent years, about their
potential hazards to human health, more precisely their pro-inflammatory effects
within the gastrointestinal system. Therefore a review of the literature has been
undertaken to understand the pro-inflammatory potential of AgNP, after human oral
exposure, in the intestine. Despite the paucity of information reported in the
literature about this issue, existing studies indicate that AgNP exert a pro-
inflammatory action, through generation of oxidative stress, accompanied by
mitochondrial dysfunction, interference with transcription factors and production
of cytokines. However, further studies are needed to elucidate the mechanistic
pathways and molecular targets involved in the intestinal pro-inflammatory effects
of AgNP.
AN - WOS:000769848400001
AU - Sousa, A.
AU - Bradshaw, T. D.
AU - Ribeiro, D.
AU - Fernandes, E.
AU - Freitas, M.
C6 - MAR 2022
DA - JUN
DO - 10.1007/s00204-022-03270-w
IS - 6
PY - 2022
SN - 0340-5761
1432-0738
SP - 1551-1571
ST - Pro-inflammatory effects of silver nanoparticles in the intestine
T2 - ARCHIVES OF TOXICOLOGY
TI - Pro-inflammatory effects of silver nanoparticles in the intestine
VL - 96
ID - 5910
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are the most widely produced type of
nanoparticles due to their antimicrobial and preservative properties. However,
their systemic bioavailability may be considered a potential hazard. When AgNP
reach the bloodstream, they interact with the immune cells, contributing to the
onset and development of an inflammatory response. Monocytes and macrophages play a
pivotal role in our defense system, but the interaction of AgNP with these cells is
still not clear. Therefore, the main objective of this work was to assess the
cytotoxic and pro-inflammatory effects induced by 5, 10, and 50 nm AgNP coated with
polyvinylpyrrolidone (PVP) and citrate, in concentrations that could be attained in
vivo (0-25 mu g/mL), in human monocytes isolated from human blood and human
macrophages derived from a monocytic cell line (THP-1). The effects of PVP and
citrate-coated AgNP on cell viability, mitochondrial membrane potential, and
cytokines release were evaluated. The results evidenced that AgNP exert strong
harmful effects in both monocytes and macrophages, through the establishment of a
strong pro-inflammatory response that culminates in cell death. The observed
effects were dependent on the AgNP concentration, size and coating, being observed
more pronounced cytotoxic effects with smaller PVP coated AgNP. The results showed
that human monocytes seem to be more sensitive to AgNP exposure than human
macrophages. Considering the increased daily use of AgNP, it is imperative to
further explore the adverse outcomes and mechanistic pathways leading to AgNP-
induced pro-inflammatory effects to deep insight into the molecular mechanism
involved in this effect.
AN - WOS:000887888100001
AU - Sousa, A.
AU - Rufino, A. T.
AU - Fernandes, R.
AU - Malheiro, A.
AU - Carvalho, F.
AU - Fernandes, E.
AU - Freitas, M.
C6 - NOV 2022
DA - FEB
DO - 10.1007/s00204-022-03415-x
IS - 2
PY - 2023
SN - 0340-5761
1432-0738
SP - 405-420
ST - Silver nanoparticles exert toxic effects in human monocytes and macrophages
associated with the disruption of Delta psi m and release of pro-inflammatory
cytokines
TI - Silver nanoparticles exert toxic effects in human monocytes and macrophages
associated with the disruption of Delta psi m and release of pro-inflammatory
cytokines
VL - 97
ID - 5966
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are the most widely produced type of
nanoparticles due to their antimicrobial and preservative properties. However,
their systemic bioavailability may be considered a potential hazard. When AgNP
reach the bloodstream, they interact with the immune cells, contributing to the
onset and development of an inflammatory response. Monocytes and macrophages play a
pivotal role in our defense system, but the interaction of AgNP with these cells is
still not clear. Therefore, the main objective of this work was to assess the
cytotoxic and pro-inflammatory effects induced by 5, 10, and 50 nm AgNP coated with
polyvinylpyrrolidone (PVP) and citrate, in concentrations that could be attained in
vivo (0–25 μg/mL), in human monocytes isolated from human blood and human
macrophages derived from a monocytic cell line (THP-1). The effects of PVP and
citrate-coated AgNP on cell viability, mitochondrial membrane potential, and
cytokines release were evaluated. The results evidenced that AgNP exert strong
harmful effects in both monocytes and macrophages, through the establishment of a
strong pro-inflammatory response that culminates in cell death. The observed
effects were dependent on the AgNP concentration, size and coating, being observed
more pronounced cytotoxic effects with smaller PVP coated AgNP. The results showed
that human monocytes seem to be more sensitive to AgNP exposure than human
macrophages. Considering the increased daily use of AgNP, it is imperative to
further explore the adverse outcomes and mechanistic pathways leading to AgNP-
induced pro-inflammatory effects to deep insight into the molecular mechanism
involved in this effect. © 2022, The Author(s), under exclusive licence to
Springer-Verlag GmbH Germany, part of Springer Nature.
AU - Sousa, A.
AU - Rufino, A. T.
AU - Fernandes, R.
AU - Malheiro, A.
AU - Carvalho, F.
AU - Fernandes, E.
AU - Freitas, M.
DB - Scopus
DO - 10.1007/s00204-022-03415-x
IS - 2
KW - Human macrophages
Human monocytes
Inflammation
Toxicity
Citrates
Citric Acid
Cytokines
Humans
Macrophages
Metal Nanoparticles
Monocytes
Povidone
Silver
citric acid
interleukin 10
interleukin 12p70
interleukin 1beta
interleukin 6
interleukin 8
povidone
silver nanoparticle
cytokine
metal nanoparticle
silver
adverse outcome
apoptosis
Article
cell death
cell isolation
cell ultrastructure
cell viability
coating (procedure)
cytokine release
flow cytometry
Golgi complex
human
human cell
in vivo study
inflammation
macrophage
monocyte
nanotoxicology
physical chemistry
propidium iodide assay
THP-1 cell line
M3 - Article
PY - 2023
SP - 405-420
ST - Silver nanoparticles exert toxic effects in human monocytes and macrophages
associated with the disruption of Δψm and release of pro-inflammatory cytokines
TI - Silver nanoparticles exert toxic effects in human monocytes and macrophages
associated with the disruption of Δψm and release of pro-inflammatory cytokines
85142511559&doi=10.1007%2fs00204-022-03415-
x&partnerID=40&md5=4c7e3037721c1347d8b651c299abe175
VL - 97
ID - 4993
ER -
TY - JOUR
AB - BACKGROUND: There are no records of comparative studies on the immunological,
histological and immunohistochemical aspects of vitiligo, halo nevus and vitiligoid
variant of lupus erythematosus in the literature. The studies available present
only descriptive clinical data on leucoderma that accompanies lupus erythematosus
in its diverse clinical forms. OBJECTIVES: 1- To evaluate the immunohistochemical
differences between vitiligo, halo nevus and vitiligoid variant of lupus
erythematosus; 2- To verify whether the depigmentation observed in the diverse
clinical forms of lupus is due to post-inflammatory destruction or to specific
immunological attack on melanocytes. METHODS: 1- Detection of melanocyte
antibodies: by direct and indirect immunofluorescence on nevus and melanoma cells;
2- Cytotoxicity evaluation: study of the activity of NK cells against cultivated
melanoma cells; 3- Histopathological study of melanocytes and melanin:
histopathology with hematoxylin-eosin, Fontana- Masson, Dopa and Dopa + silver and
S-100 protein test by immunoperoxidase. RESULTS: Vitiligo and halo nevus patients
presented to antimelanocyte antibodies in 25% of cases. Patients with vitiligoid
variant of lupus erythematous also presented these antibodies. The presence of risk
factors favoring cellular cytotoxicity was demonstrated in vitiligo and/or halo
nevus, as well as in the vitiligoid variant of lupus erythematous. Staining with
Dopa + silver nitrate was superior to traditional staining and to S-100 protein to
detect melanocytes and/or melanin in depigmented lesions of vitiligo and/or halo
nevus and vitiligoid variant of lupus erythematous. CONCLUSION: The results confirm
the existence of antimelanocyte antibodies in vitiligo and halo nevus. It is not
possible to rule out some immunological phenomena similar to those occurring in
vitiligo and halo nevus in the genesis of vitiligoid lesions in lupus erythematous.
The detection of melanocytes in achromic lesions of vitiligo suggests the
predominance of a functional inhibitory mechanism rather than cell destruction in
the genesis of the disease. ©2005 by Anais Brasileiros de Dermatologia.
AU - Souza Filho, L. G. C.
AU - Rivitti, E. A.
AU - Miyauchi, L. M.
AU - Sotto, M. N.
AU - Maria, D. A.
AU - Puejo, S. S. T.
AU - Alves, V. A. F.
DB - Scopus
DO - 10.1590/s0365-05962005000200004
IS - 2
KW - Allergy and immunology
Lupus
Nevus, pigmented
Vitiligo
cell antibody
DOPA
eosin
hematoxylin
melanin
melanocyte antibody
peroxidase
protein S 100
silver
unclassified drug
antibody detection
article
clinical feature
comparative study
cytotoxicity
depigmentation
halo nevus
histopathology
human
immunofluorescence
immunopathology
lupus erythematosus
melanocyte
natural killer cell
nevus
risk factor
vitiligo
vitiligoid variant of lupus erythematosus
M3 - Article
PY - 2005
SP - 143-148
ST - Estudo comparativo entre vitiligo, nevo halo e lúpus eritematoso vitiligóide
por meio de métodos imunológicos, histológicos e imuno-histoquímicos
T2 - Anais Brasileiros de Dermatologia
TI - Comparative study of vitiligo, halo nevus, and vitiligoid variant of lupus
erythematosus by immunological, histological, and immunohistochemical methods
20344391390&doi=10.1590%2fs0365-
05962005000200004&partnerID=40&md5=2292786e1cfa79bb11eff78de40e1550
VL - 80
ID - 5810
ER -
TY - GEN
AB - O objetivo deste estudo foi avaliar a atividade antimicrobiana/antibiofilme e
a viabilidade celular de nanopartículas de prata (NPsAg) obtidas por uma síntese
'green' associadas ou não ao ß-glicerofosfato de cálcio (GPCa) contra Streptococcus
mutans e espécies de Candida (cepas de referência e isolados clínicos orais
incluindo cepas resistentes ao fluconazol). O efeito destes nanocompostos em
combinação com o tirosol (TIR), fluconazol (FLC), nistatina (NIT) e anfotericina B
(AnB) também foi avaliado. Além disso, nós avaliamos comparativamente as alterações
do transcriptoma de células de C. glabrata CBS138 após exposição às NPsAg e ao Íon
prata (Ag+ ). Inicialmente, as NPsAg foram sintetizadas por meio da redução do
nitrato de prata com extratos de diferentes partes (casca, folha e semente) de uma
romã (Punica granatum L.) associadas ou não ao GPCa. A Concentração Inibitória
Mínima (CIM) e as Concentrações Fungicida/Bactericida Mínima (CFM e CBM) dos
nanocompostos (NPsAg e NPsAgGPCa) associados ou não ao TIR contra S. mutans e C.
albicans foram determinadas pelo método de microdiluição. E, a ação das NPsAg
combinadas com FLC, NIT e AnB também foi avaliada em condição planctônica contra
isolados clínicos orais de espécies de Candida. O efeito dos nanocompostos
associados ou não ao TIR sobre a viabilidade celular de fibroblastos da linhagem
L929 e a produção de citocinas foi avaliado através dos ensaios de MTT e ELISA,
respectivamente. O número de hifas foi quantificado em biofilmes de C. albicans
formados na presença das NPsAg com ou sem soro fetal bovino (SFB). O efeito das
NPsAg em inibir a formação do biofilme (em C. albicans e C. glabrata) também foi
investigado através do ensaio de PrestoBlue e por meio da microscopia eletrônica de
varredura. Os nanocompostos (NPsAg e NPsAg-GPCa) associados ou não ao TIR foram
aplicados sobre biofilmes de S. mutans e de espécies de Candida (12 e 24 h) e, após
24 h de contato, sua atividade antibiofilme foi determinada por meio da enumeração
das unidades formadoras de colônias (UFCs) e ensaio de PrestoBlue. Além disso, uma
análise transcriptômica foi realizada utilizando microchips de DNA ('microarrays')
a fim de avaliar quais genes estão mais ou menos expressos como resposta às NPsAg
(no estado planctônico e formando biofilmes) e ao Íon Ag+ (formando biofilmes). As
soluções antimicrobianas sintetizadas neste estudo (NPsAg e NPsAgGPCa) apresentaram
atividade antimicrobiana contra os microrganismos testados. Além disso, menores
valores de CIM foram obtidos quando estes nanocompostos foram associados ao TIR,
FLC, NIT e AnB apresentando um efeito sinérgico. NPsAg e NPsAgGPCa não foram
tóxicas às células L929, aumentaram a produção de fator de crescimento celular e
não promoveram alterações significativas na liberação de Interleucina-6. Os
nacompostos associados ao TIR não apresentaram efeito citotóxico sobre culturas de
L929, exceto para a maior concentração (NPsAg 39,05 µg/mL + TIR 1,25 mM). A
presença das NPsAg reduziu drasticamente o número de hifas em biofilmes de C.
albicans na presença ou na ausência de SFB. A quantidade de biofilme das espécies
de Candida formado na presença das NPsAg reduziu para mais de 50%. Após 24 h de
tratamento com os nanocompostos (NPsAg e NPsAg-GPCa) em biofilmes de S. mutans,
houve uma redução significativa no número de UFCs sendo similar à clorexidina. Uma
redução na viabilidade de biofilmes de C. glabrata também foi observada após
exposição às NPsAg (24 h). Entretanto, os nanocompostos (NPsAg e NPsAg-GPCa)
associados ou não ao TIR não foram efetivos contra biofilmes de C. albicans. Após
exposição às NPsAg e ao Íon Ag+ , alterações no transcriptoma de células de C.
glabrata CBS138 foram observadas: no estado planctônico, houve uma superexpressão
dos genes responsáveis pela biossíntese de metionina e de lisina e uma subexpressão
dos genes relacionados com o transporte transmembrana; e, as células expostas às
NPsAg responder m de forma distinta em relação ao Íon Ag+ , indicando que as NPsAg
podem apresentar um mecanismo de ação diferente. Estes achados podem auxiliar nas
decisões terapêuticas com formulações contendo NPsAg ou NPsAg-GPCa associadas ou
não a diferentes drogas em pacientes com cárie dentária e candidíase oral(AU)
The aim of this study was to evaluate the antimicrobial/antibiofilm activities and
the cell viability of silver nanoparticles (AgNPs) obtained by a 'green' synthesis
associated or not to ß-calcium glycerophosphate (CaGP) against Streptococcus mutans
and Candida species (reference strains and oral clinical isolates including azole-
resistant strains). The effect of these nanocompounds in combination with tyrosol
(TYR), fluconazole (FLC), nystatin (NYT) and amphotericin B (AmB) also was
evaluated. Furthermore, we evaluated, comparatively, the transcriptome alterations
of cells of C. glabrata CBS138 after exposition to AgNPs and to silver ion (Ag+ ).
Initially, AgNPs were synthesized by reducing silver nitrate with extracts of
different parts (peel, leaves and seeds) of a pomegranate (Punica granatum L.)
associated or not to CaGP. Minimum Inhibitory Concentration (MIC) and Minimum
Fungicidal/Bactericidal Concentrations (MFC and MBC) of the nanocomposites (AgNPs
and AgNPs-CaGP) associated or not to TYR against S. mutans and C. albicans were
determined by the microdilution method. And, the action of the AgNPs combined with
FLC, NYT and AmB also was evaluated in planktonic condition against oral clinical
isolates of Candida species. The effect of the nanocomposites associated or not to
TYR on cell viability of fibroblasts (L929) and cytokines production was evaluated
through MTT and ELISA assays, respectively. The number of cells undergoing
filamentation was quantified in C. albicans biofilms formed in the presence of
AgNPs with or without fetal bovine serum (FBS). The effect of AgNPs in inhibit the
formation of biofilm (in C. albicans and C. glabrata) also was investigated through
PrestoBlue assay and scanning electron microscope. Biofilms of S. mutans and
Candida species (12 and 24 h) were treated with nanocomposites (AgNPs and
AgNPsCaGP) associated or not to TYR for 24 h and, then, the viability of these
biofilms was determined through PrestoBlue assay and colony forming units (CFUs).
Moreover, a transcriptome analysis was performed using microarrays to determine
which genes are up- or down-regulated as response to AgNPs (in the planktonic state
and forming biofilms) and to Ag+ ion (forming biofilms). Antimicrobial solutions
synthesized in this study (AgNPs and AgNPs-CaGP) presented antimicrobial activity
against tested microorganisms. Furthermore, lower values of MIC were obtained when
these nanocompounds were associated to TYR, FLC, NYT and AmB showing a synergistic
effect. AgNPs and AgNPs-CaGP were not toxic to L929 cells, increased the stem cell
factor production and did not promote significant alterations in the Interleukine-6
release. The nanocomposites associated to TYR did not present cytotoxic effect on
L929 cultures, except for the higher concentration (AgNPs 39.05 µg/mL + TYR 1.25
mM). The incubation in the presence of the AgNPs drastically reduced the number of
cells exhibiting hyphae, this effect being observed either in the presence or
absence of FBS. The amount of biofilm formed by Candida species in the presence of
the AgNPs was reduced by more than 50% the one formed in the absence of the
nanoparticles, this reduction being further increased to more than 90% when the
concentration of the AgNPs was increased. After 24 h of treatment with the
nanocompounds (AgNPs and AgNPs-CaGP) in S. mutans biofilms, there was a significant
reduction in the number of CFUs being similar to chlorhexidine. A reduction in the
viability of C. glabrata biofilms also was observed after exposition to AgNPs (24
h). However, the nanocomposites (AgNPs and AgNPs-CaGP) associated or not to TYR
were not effective against C. albicans biofilms. After exposition to AgNPs and to
Ag+ ion, alterations in the transcriptome of cells of C. glabrata CBS138 were
observed: in the planktonic state, genes responsible by the methionine and lysine
biosynthesis are up-regulated, and genes related with the transmembrane transport
are down-regulated. And, finally, the cells exposed to AgNPs responded differently
in relation to the Ag+ ion, indi ating that the AgNPs might present a different
mechanism of action. All these results may help guide therapeutic decisions with
formulation containing AgNPs or AgNPs-CaGP associated or not with different
compounds in patients with dental caries and oral candidiasis(AU)
AU - Souza, José Antonio Santos
C1 - 20190819
C8 - biblio-1009528
DA - 2018/00
DB - LILACS
KW - Candidíase Bucal
Cárie Dentária
Dental Caries
Nanotechnology
Nanotecnologia
Oral Candidiasis
Prata
Silver
Toxicidade
Toxicity
LA - pt
PY - 2018
SP - 186-186
ST - Nanopartículas de prata obtidas pela síntese 'green' combinadas ou não com
glicerofosfato de cálcio e tirosol: efeito antimicrobiano, anti-inflamatório e na
resposta transcriptômica em patógenos orais
TI - Nanopartículas de prata obtidas pela síntese 'green' combinadas ou não com
glicerofosfato de cálcio e tirosol: efeito antimicrobiano, anti-inflamatório e na
resposta transcriptômica em patógenos orais
TT - Silver nanoparticles obtained by the 'green' synthesis combined or not with
calcium glycerophosphate and tyrosol: antimicrobial, anti-inflammatory effect and
in the transcriptomic response in oral pathogens
UR - https://repositorio.unesp.br/handle/11449/165285
ID - 4927
ER -
TY - JOUR
AB - Percutaneous silver wire implants were looped through the dorsal skin of rats
and inoculated with Staphylococcus aureus to test the effect on bacteria in the
tract. The silver was activated with four brief daily applications of anodic
microcurrent. Contralateral 316L stainless steel implants, identically inoculated,
served as controls. Cultures from the silver tracts showed a marked reduction or
elimination of bacteria, which persisted for the 3‐week study period. In tracts
with colonization established for 1 week, subsequent electrical activation of the
silver also suppressed the bacteria. Inflammatory reactions at 3 weeks were mild at
both the silver and stainless implants and no giant cells or toxicity were seen.
This suggests that electrically activated silver may be useful in preventing or
treating infection at percutaneous devices. Copyright © 1986 John Wiley & Sons,
Inc.
AU - Spadaro, J. A.
AU - Chase, S. E.
AU - Webster, D. A.
DB - Scopus
DO - 10.1002/jbm.820200504
IS - 5
Animal
Drug Implants
Electric Conductivity
Electric Stimulation
Kinetics
Rats
Silver
Time Factors
BACTERIOLOGY
WIRE - Silver
silver
stainless steel
adverse drug reaction
animal experiment
animal model
drug comparison
drug efficacy
drug sterility
electrostimulation
histology
metal implantation
nonhuman
prevention
rat
skin infection
staphylococcus aureus
therapy
ANODIC MICROCURRENT
BACTERIAL INHIBITION
PERCUTANEOUS IMPLANTS
STAPHYLOCOCCUS AUREUS
BIOMEDICAL ENGINEERING
M3 - Article
PY - 1986
SP - 565-577
ST - Bacterial inhibition by electrical activation of percutaneous silver implants
T2 - Journal of Biomedical Materials Research
TI - Bacterial inhibition by electrical activation of percutaneous silver implants
0022718916&doi=10.1002%2fjbm.820200504&partnerID=40&md5=cf0bb1d03ca762bbef695cf4f1a
223c8
VL - 20
ID - 5816
ER -
TY - JOUR
AB - Evolution of metal implants progressively shifted the focus from adequate
mechanical strength to improved biocompatibility and absence of toxicity and,
finally, to fast osseointegration. Recently, new frontiers and challenges of Ti
implants have been addressed to improvement of bioactivity, fighting of bacterial
infection and biofilm formation, as well as modulation of inflammation. This is
closely related to the clinical demand of multifunctional implants able to
simultaneously have a number of specific responses with respect to body fluids,
cells (osteoblasts, fibroblasts, macrophages) and pathogenic agents (bacteria,
viruses). This complex system of multiple biological stimuli and surface responses
is a major arena of the current research on biomaterials and biosurfaces. This
review covers the strategies explored to this purpose since 2010 in the case of Ti
and Ti alloys, considering that the number of related papers doubled about in the
last seven years and no review has comprehensively covered this engaging research
area yet. The different approaches followed for producing multifunctional Ti-based
surfaces involve the use of thick and thin inorganic coatings, chemical surface
treatments, and functionalization strategies coupled with organic coatings.
Statement of Significance According to the clinical demand of multifunctional
implants able to simultaneously have a number of specific responses with respect to
body fluids, cells and pathogenic agents, new frontiers of Ti implants have been
addressed to improvement of bioactivity, fighting of bacterial infection and
biofilm formation, as well as modulation of inflammation. Literature since 2010 is
here reviewed. Several strategies for getting bioactive and antibacterial actions
on Ti surfaces have been suggested, but they still need to be optimized with
respect to several concerns. A further step will be to combine on the same surface
a proven ability of modulation of inflammatory response. The achievement of
multifunctional surfaces able to modulate inflammation and to promote osteogenesis
is a grand challenge. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All
rights reserved.
AN - WOS:000447477600001
AU - Spriano, S.
AU - Yamaguchi, S.
AU - Baino, F.
AU - Ferraris, S.
DA - OCT 1
DO - 10.1016/j.actbio.2018.08.013
PY - 2018
SN - 1742-7061
1878-7568
SP - 1-22
ST - A critical review of multifunctional titanium surfaces: New frontiers for
improving osseointegration and host response, avoiding bacteria contamination
TI - A critical review of multifunctional titanium surfaces: New frontiers for
improving osseointegration and host response, avoiding bacteria contamination
VL - 79
ID - 6750
ER -
TY - JOUR
AB - Tumour Necrosis Factor (TNF-α) is a pro-inflammatory cytokine having key
roles in cell death, differentiation, survival, proliferation, migration and is a
modulator of immune system. Therefore, TNF-α is an ideal biomarker for several
disease diagnosis including cancer. However, out of all the biomarkers of cancer,
TNF-α) is less explored for cancer detection. Only a few reports are available of
developing biosensors for TNF-α targeting in human serum samples. Also, Carbon Dots
(CDs) remains less explored in biosensor application. In this regard, for the first
time, a sensitive and low-cost electrochemical biosensor based on CDs has
developed. CDs were synthesized by simple yet facile microwave pyrolysis. Poly
methyl methacrylate (PMMA) was selected as the matrix to hold CDs to fabricate the
biosensing platform. This novel CD-PMMA nanocomposite featuring excellent
biocompatibility, exceptional electrocatalytic conductivity, and large surface
area. CD-PMMA was applied as transducing material to efficiently conjugate
antibodies specific towards TNF-α and fabricate electrochemical immunosensor for
specific detection of TNF-α. The fabricated immunosensor was used for the detection
of TNF-α within a wide dynamic range of 0.05–160 pg mL−1 with a lower detection
limit of 0.05 pg mL−1 and sensitivity of 5.56 pg mL−1 cm−2. Furthermore, this CDs
based immunosensor retains high sensitivity, selectivity, and stability. This
immunosensor demonstrated a high correlation with the conventional technique,
Enzyme-Linked Immunosorbent Assay for early screening of cancer patient serum
samples. © 2021 Elsevier B.V.
AU - Sri, S.
AU - Lakshmi, G. B. V. S.
AU - Gulati, P.
AU - Chauhan, D.
AU - Thakkar, A.
AU - Solanki, P. R.
C7 - 338909
DB - Scopus
DO - 10.1016/j.aca.2021.338909
KW - Cancer diagnosis
Carbon dots
Composite matrix
Electrochemical biosensor
PMMA
TNF-α
Carbon
Electrochemical Techniques
Humans
Immunoassay
Limit of Detection
Neoplasms
Biocompatibility
Biomarkers
Body fluids
Cell death
Chemical detection
Diagnosis
Esters
Immunosensors
1 (3 dimethylaminopropyl) 3 ethylcarbodiimide
acetic acid
biological marker
carbon
citric acid
cysteine
ferric ferrocyanide
formic acid
n hydroxysuccinimide
nanocomposite
potassium ferricyanide
silver chloride
sodium chloride
Cancer patients
Composite matrices
Poly(methyl methacrylate)
Poly-methyl methacrylates
Serum samples
Simple++
adult
amperometry
Article
biocompatibility
calibration
calorimetry
cancer diagnosis
cancer survival
cell death
cell migration
cell proliferation
cell survival
centrifugation
chemical parameters
contact angle
controlled study
female
human
immobilization
immune system
impedance spectroscopy
kinetics
limit of detection
male
malignant neoplasm
microwave radiation
mouth cancer
nonhuman
normal human
pH
photoluminescence
pyrolysis
Raman spectrometry
sensitivity and specificity
genetic procedures
immunoassay
neoplasm
Diseases
M3 - Article
PY - 2021
ST - Simple and facile carbon dots based electrochemical biosensor for TNF-α
targeting in cancer patient's sample
T2 - Analytica Chimica Acta
TI - Simple and facile carbon dots based electrochemical biosensor for TNF-α
targeting in cancer patient's sample
85113484328&doi=10.1016%2fj.aca.2021.338909&partnerID=40&md5=93ad9664347760607a197a
bc307ddd20
VL - 1182
ID - 5167
ER -
TY - JOUR
AB - Human health is severely hampered by a majority of the neurological disorders
such as the brain tumors, degenerative Alzheimer's disease, Parkinson's disease and
those involving inflammatory component. Owing to the stringent protection offered
by the blood brain barrier, conventional therapeutics gain limited access and
therefore, are therapeutically suboptimal. Hence, research has now focused to
develop the novel drug delivery systems with a prime motto of maintaining
therapeutic drug levels inside the brain, avoiding non-specific tissue
distribution. The introduction of nanotechnology has addressed few of these
objectives and opened up new avenues for even more improvization. To some extent,
nanodelivery systems were successful in crossing the blood brain barrier and
accessing the remote areas of the brain. They also have shown tremendous potential
in delivering the therapeutic and diagnostic aids following systemic
administration. What revolutionised the nano applications is the development of
"smart" nanosystems, whose surface is tailor made for the effective theranostic
delivery. However, a detailed understanding of the long term nanoformulation
toxicities, along with the neuropathology, is the critical future question to be
addressed. In this review, a brief introduction of the prominent neurological
disorders and detailed applications of nanotechnology are discussed. © 2014 Bentham
Science Publishers.
AU - Sriramoju, B.
AU - Kanwar, R. K.
AU - Kanwar, J. R.
DB - Scopus
DO - 10.2174/0929867321666140716095644
IS - 36
KW - Blood brain barrier
Nanomedicine
Neurological disorders
Targeted therapeutics
Blood-Brain Barrier
Drug Carriers
Humans
Nanoparticles
Nervous System Diseases
Polymers
abs 75
benzyloxycarbonylaspartylglutamylvalylaspartyl fluoromethyl ketone
carbon nanofiber
carbon nanotube
dendrimer
dextran magnetite
doxorubicin
glial cell line derived neurotrophic factor
glutamate receptor antagonist
gold nanoparticle
liposome
metal nanoparticle
molecular scaffold
monoclonal antibody
monocrystalline iron oxide nanoparticle
nanocapsule
nanoliposome
nanoparticle
nanosphere
nerve growth factor
paclitaxel
penicillamine
polyamidoamine
polymer
silver nanoparticle
transactivator protein
unclassified drug
unindexed drug
drug carrier
adsorption
Alzheimer disease
Article
blood brain barrier
cerebrovascular accident
covalent bond
drug brain level
drug formulation
drug mechanism
drug penetration
drug structure
drug targeting
drug transport
gel
glioma
human
micelle
multiple sclerosis
nanoformulation
nanogel
nanomedicine
nanomicelle
nanosuspension
nerve regeneration
neuroimaging
neurologic disease
neuropathology
nonhuman
oxidative stress
Parkinson disease
suspension
tumor localization
chemistry
metabolism
M3 - Article
PY - 2014
SP - 4154-4168
ST - Nanomedicine based nanoparticles for neurological disorders
T2 - Current Medicinal Chemistry
TI - Nanomedicine based nanoparticles for neurological disorders
84928654680&doi=10.2174%2f0929867321666140716095644&partnerID=40&md5=2a606634350275
fd67415b7177830821
VL - 21
ID - 5598
ER -
TY - JOUR
AB - Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used
nanomaterials. Following oral exposure, these NPs can accumulate in various organs
and induce the toxicity due to their physiochemical characteristics. In present
study to reduce the toxicity, surface engineered ZnO NPs (c-ZnO NPs) were in-situ
synthesized by using polyacrylamide grafted guar gum (PAm-g-GG) polymer in alkaline
media. Further, the comparative effect of bared ZnO NPs (b-ZnO NPs) and c-ZnO NPs
were assessed on secondary target organ liver and kidneys of Swiss mice at doses of
10, 50 and 300 mg/kg following 28 days repeated oral treatment. The b-ZnO NPs were
incited severe damages in liver and kidney tissue than c-ZnO NPs as seen by
transmission electron microscopy and histopathology. The increased levels of serum
biomarkers (AST, ALT, ALP, creatinine, uric acid, and urea) were also observed,
that remarking a disturbance in the function of liver and kidney. After sub-acute
oral treatment of b-ZnO NPs, the hepatic pro-inflammatory cytokines (IL-6, TNF-
alpha, and MMP-9) were up-regulated that causes the activation of acute phase
response (APR). We also observed significantly increased in expression of hepatic
acute phase proteins (hepcidin and haptoglobin) and altered interlinked iron (Fe)
signaling biomarkers (hephaestin, IT, TFR-1, LDH, and ferroportin). This study
emphasizes that exposure to ZnO NPs may cause inflammation mediated APR through
ultra-structural damage of tissue that could escort the progression of anemia.
Nevertheless, the capping with PAm-g-GG in c- ZnO NPs has reduced the toxicity by
altering the surface reactive property of ZnO NPs.
AN - WOS:000463958300036
AU - Srivastav, A. K.
AU - Dhiman, N.
AU - Tiwari, R.
AU - Arjaria, N.
AU - Prakash, J.
AU - Jagdale, P.
AU - Ayanur, A.
AU - Singh, D.
AU - Patnaik, S.
AU - Kumar, M.
DO - 10.1016/j.jtemb.2019.01.008
PY - 2019
SN - 0946-672X
SP - 270-287
ST - Sub-acute oral exposure of zinc oxide nanoparticles causes alteration in iron
homeostasis through acute phase response: A protective effect by surface
modification
T2 - JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
TI - Sub-acute oral exposure of zinc oxide nanoparticles causes alteration in iron
homeostasis through acute phase response: A protective effect by surface
modification
VL - 52
ID - 6703
ER -
TY - JOUR
AB - Nickel titanium (NiTi, or Nitinol) alloy is used in several biomedical
applications, including cardiac, peripheral vascular, and fallopian tube stents.
There are significant biocompatibility issues of metallic implants to nickel ions
and nano-/micro-sized alloy particles. Our laboratories have recently shown that
microscale CoCr wear particles from metal-on-metal hips crosslink with the innate
immune signaling Toll-like receptor 4 (TLR4), prompting downstream signaling that
results in interleukin (IL)-1 beta and IL-8 gene expression. In vivo, NiTi alloy
can also generate wear particles on the nanoscale (NP) that have thus far not been
studied for their potential to induce inflammation and angiogenesis that can, in
turn, contribute to implant (e.g. stent) failure. Earlier studies by others
demonstrated that nickel could induce contact hypersensitivity by crosslinking the
human, but not the mouse, TLR4. In the present work, it is demonstrated that NiCl2
ions and NiTi nanoparticles induce pro-inflammatory and pro-angiogenic
cytokine/chemokine expression in human endothelial and monocyte cell lines in
vitro. These observations prompt concerns about potential mechanisms for stent
failure. The data here showed a direct correlation between intracellular uptake of
Ni2+ and generation of reactive oxygen species. To determine a role for nickel and
NiTi nanoparticles in inducing angiogenesis in vivo, 1-cm silicone angioreactors
were implanted subcutaneously into athymic (T-cell-deficient) nude mice. The
angioreactors contained Matrigel (a gelatinous protein mixture that resembles
extracellular matrix) in addition to one of the following: PBS (negative control),
VEGF/FGF-2 (positive control), NiCl2, or NiTi NP. The implantation of angioreactors
represents a potential tool for quantification of angiogenic potentials of medical
device-derived particles and ions in vivo. By this approach, NiTi NP were found to
be markedly angiogenic, while Ni2+ was less-so. The angioreactors may provide a
powerful tool to examine if debris shed from medical devices may promote untoward
biological effects.
AN - WOS:000832576500001
AU - Srivastava, A. K.
AU - Snapper, D. M.
AU - Zheng, J. W.
AU - Yildrim, B. S.
AU - Srivastava, S.
AU - Wood, S. C.
DA - DEC 31
DO - 10.1080/1547691X.2022.2080307
IS - 1
PY - 2022
SN - 1547-691X
1547-6901
SP - 61-73
ST - Examining the role of nickel and NiTi nanoparticles promoting inflammation
and angiogenesis
TI - Examining the role of nickel and NiTi nanoparticles promoting inflammation
and angiogenesis
VL - 19
ID - 6784
ER -
TY - JOUR
AB - With tremendous increase in development of nanotechnology, there is a
developing enthusiasm toward the application of nanoparticles in diverse areas.
Carbon nanotubes, fullerenes, quantum dots, dendrimers, iron oxide, silica, and
gold and silver nanoparticles are frequently used in different applications such as
drug delivery, ceramic materials, semiconductors, electronics, medicine, cosmetics,
etc. Some of these nanoparticles have shown major toxic effects on fauna, flora,
and human beings, such as inflammation, cytotoxicity, tissue ulceration, and
reduction of cell viability. Single-walled carbon nanotubes (SWCNTs) and
multiwalled carbon nanotubes (MWCNTs) can induce oxidative stress and fibrosis in
the lungs of rat and mice. SWCNTs can also induce oxidative stress to the nervous
system in human beings. Inflammatory injury and respiratory distress can be
observed due to TiO2 nanoparticles with small diameter. Nanoparticles can also pose
detrimental effects on plants, such as decreased growth rate, genomic and proteomic
changes, etc. Toxicity of nanoparticles arises because of their specific
characteristics, such as greater 'surface area to volume ratio' compared with bulk
particles of the same chemistry. The objective of this review is to critically
evaluate the current literature on the toxicity of nanoparticles. © 2015 American
Chemical Society.
AU - Srivastava, V.
AU - Gusain, D.
AU - Sharma, Y. C.
DB - Scopus
DO - 10.1021/acs.iecr.5b01610
IS - 24
KW - Carbon
Ceramic materials
Nanoparticles
Nanotechnology
Silver
Single-walled carbon nanotubes (SWCN)
Toxic materials
Toxicity
Yarn
Cell viability
Critical review
Multiwalled carbon nanotube (MWCNTs)
Single-walled carbon nanotube (SWCNTs)
Toxic effect
Volume ratio
Multiwalled carbon nanotubes (MWCN)
M3 - Article
PY - 2015
SP - 6209-6233
ST - Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles
T2 - Industrial and Engineering Chemistry Research
TI - Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles
84933056662&doi=10.1021%2facs.iecr.5b01610&partnerID=40&md5=b2b4d3a12b6c2001f4f54ab
8710b5952
VL - 54
ID - 5617
ER -
TY - JOUR
AB - Studies suggest that the protection against SIV/simian-human immunodeficiency
virus (SHIV) acquisition afforded by the SIV/HIV V1 deletion-containing envelope
immunogens, delivered by the DNA/ALVAC vaccine platform, requires multiple innate
and adaptive host responses. Anti-inflammatory macrophages and tolerogenic
dendritic cells (DC-10), together with CD14(+) efferocytes, are consistently found
to correlate with a vaccine-induced decrease in the risk of SIV/SHIV acquisition.
The monoclonal antibodies (MAbs) NCI05 and NCI09, isolated from a vaccinated
macaque that was protected from multiple simian immunodeficiency virus (SIV)
challenges, both target an overlapping, conformationally dynamic epitope in SIV
envelope variable region 2 (V2). Here, we show that NCI05 recognizes a CH59-like
coil/helical epitope, whereas NCI09 recognizes a beta-hairpin linear epitope. In
vitro, NCI05 and, to a lesser extent, NCI09 mediate the killing of SIV-infected
cells in a CD4-dependent manner. Compared to NCI05, NCI09 mediates higher titers of
antibody-dependent cellular cytotoxicity (ADCC) to gp120-coated cells, as well as
higher levels of trogocytosis, a monocyte function that contributes to immune
evasion. We also found that passive administration of NCI05 or NCI09 to macaques
did not affect the risk of SIVmac251 acquisition compared to controls,
demonstrating that these anti-V2 antibodies alone are not protective. However,
NCI05 but not NCI09 mucosal levels strongly correlated with delayed SIVmac251
acquisition, and functional and structural data suggest that NCI05 targets a
transient state of the viral spike apex that is partially opened, compared to its
prefusion-closed conformation.IMPORTANCE Studies suggest that the protection
against SIV/simian-human immunodeficiency virus (SHIV) acquisition afforded by the
SIV/HIV V1 deletion-containing envelope immunogens, delivered by the DNA/ALVAC
vaccine platform, requires multiple innate and adaptive host responses. Anti-
inflammatory macrophages and tolerogenic dendritic cells (DC-10), together with
CD14(+) efferocytes, are consistently found to correlate with a vaccine-induced
decrease in the risk of SIV/SHIV acquisition. Similarly, V2-specific antibody
responses mediating ADCC, Th1 and Th2 cells expressing no or low levels of CCR5,
and envelope-specific NKp44(+) cells producing interleukin 17 (IL-17) also are
reproducible correlates of decreased risk of virus acquisition. We focused on the
function and the antiviral potential of two monoclonal antibodies (NCI05 and NCI09)
isolated from vaccinated animals that differ in antiviral function in vitro and
recognize V2 in a linear (NCI09) or coil/helical (NCI05) conformation. We
demonstrate that NCI05, but not NCI09, delays SIVmac251 acquisition, highlighting
the complexity of antibody responses to V2.
AN - WOS:000957781700001
AU - Stamos, J. D.
AU - Rahman, M. A.
AU - Gorini, G.
AU - de Castro, I. S.
AU - Becerra-Flores, M.
AU - Van Wazer, D. J.
AU - N'Guessan, K. F.
AU - Clark, N. M.
AU - Bissa, M.
AU - Gutowska, A.
AU - Mason, R. D.
AU - Kim, J.
AU - Rao, M. A. L.
AU - Roederer, M.
AU - Paquin-Proulx, D.
AU - Evans, D. T.
AU - Cicala, C.
AU - Arthos, J.
AU - Kwong, P. D.
AU - Zhou, T. Q.
AU - Cardozo, T.
AU - Franchini, G.
C6 - MAR 2023
DA - APR 27
DO - 10.1128/jvi.01864-22
IS - 4
PY - 2023
SN - 0022-538X
1098-5514
ST - Effect of Passive Administration of Monoclonal Antibodies Recognizing Simian
Immunodeficiency Virus (SIV) V2 in CH59-Like Coil/Helical or beta-Sheet
Conformations on Time of SIVmac251 Acquisition
T2 - JOURNAL OF VIROLOGY
TI - Effect of Passive Administration of Monoclonal Antibodies Recognizing Simian
Immunodeficiency Virus (SIV) V2 in CH59-Like Coil/Helical or beta-Sheet
Conformations on Time of SIVmac251 Acquisition
VL - 97
ID - 6628
ER -
TY - JOUR
AB - The cardiovascular response to xenobiotic particle exposure has been
increasingly studied over the last two decades, producing an extraordinary scope
and depth of research findings. With the flourishing of nanotechnology, the term
"xenobiotic particles" has expanded to encompass not only air pollution particulate
matter (PM) but also anthropogenic particles, such as engineered nanomaterials
(ENMs). Historically, the majority of research in these fields has focused on
pulmonary exposure and the adverse physiological effects associated with a host
inflammatory response or direct particle-tissue interactions. Because these
hypotheses can neither account entirely for the deleterious cardiovascular effects
of xenobiotic particle exposure nor their time course, the case for substantial
neurological involvement is apparent. Indeed, considerable evidence suggests that
not only is neural involvement a significant contributor but also a reality that
needs to be investigated more thoroughly when assessing xenobiotic particle
toxicities. Therefore, the scope of this review is several-fold. First, we provide
a brief overview of the major anatomical components of the central and peripheral
nervous systems, giving consideration to the potential biologic targets affected by
inhaled particles. Second, the autonomic arcs and mechanisms that may be involved
are reviewed. Third, the cardiovascular outcomes following neurological responses
are discussed. Lastly, unique problems, future risks, and hurdles associated with
xenobiotic particle exposure are discussed. A better understanding of these neural
issues may facilitate research that in conjunction with existing research, will
ultimately prevent the untoward cardiovascular outcomes associated with PM
exposures and/or identify safe ENMs for the advancement of human health.
AN - WOS:000365883300001
AU - Stapleton, P. A.
AU - Abukabda, A. B.
AU - Hardy, S. L.
AU - Nurkiewicz, T. R.
DA - NOV 15
DO - 10.1152/ajpheart.00546.2015
IS - 10
PY - 2015
SN - 0363-6135
1522-1539
SP - H1609-H1620
ST - Xenobiotic pulmonary exposure and systemic cardiovascular response via
neurological links
T2 - AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
TI - Xenobiotic pulmonary exposure and systemic cardiovascular response via
neurological links
VL - 309
ID - 6824
ER -
TY - JOUR
AB - The known metallotherapeutic [Ag(salH)]2 (AGSAL-1) of salicylic acid (salH2),
was used for the development of new efficient silver based material for wounds
healing. AGSAL-1 was characterized by spectroscopic techniques and X-ray
crystallography. The wound healing epithelialization of AGSAL-1 was investigated by
the means of scratch assay against immortalized human keratinocytes (HaCaT) cells.
The anti-inflammatory activity of AGSAL-1 was evaluated by monitoring the catalytic
peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme
lipoxygenase (LOX). The antibacterial activity of AGSAL-1 was evaluated against
bacterial species which colonize wounds, such as: Pseudomonas aeruginosa (PAO1),
Staphylococcus epidermidis and Staphylococcus aureus, by the means of Minimum
Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and their
Inhibition Zone (IZ). Moreover, the influence of AGSAL-1 against the formation of
biofilm of PAO1 and St. aureus was also evaluated by the mean of Biofilm
Elimination Concentration (ΒΕC). A hydrogel material CMC@AGSAL-1, based on the
dispersion of AGSAL-1 in to carboxymethyl cellulose (CMC) was tested for its
antimicrobial activity. Molecular Docking was performed, to explore the molecular
interaction of AGSAL-1 with (i) the transcriptional regulator of PAO1, LasR. (ii)
the mevalonate pathway for the biosynthesis of isoprenoids which is essential for
gram-positive bacteria St. epidermidis and St. aureus. The toxicity of AGSAL-1 was
examined against the HaCaT cells. Its genotoxicity was evaluated using Allium cepa
model, in vivo. No genotoxicity was detected, indicating that AGSAL-1 is a
candidate towards the development on a new efficient medication of the silver based
metallodrugs. © 2018 Elsevier Inc.
AU - Stathopoulou, M. E. K.
AU - Banti, C. N.
AU - Kalampounias, A. G.
DB - Scopus
DO - 10.1016/j.jinorgbio.2018.01.004
Antibacterial
Bioinorganic materials chemistry
Salicylic acid
Silver(I) complexes
Wound healing
Bacterial Proteins
Biofilms
Cell Line
Crystallography, X-Ray
Disk Diffusion Antimicrobial Tests
Humans
Hydrogels
Keratinocytes
Ligands
Molecular Conformation
Mutagenicity Tests
Salicylic Acid
Silver
Skin Cream
Staphylococcus
Trans-Activators
Wound Healing
antiinfective agent
linoleic acid
lipoxygenase
metal complex
salicylic acid
silver complex
silver nitrate
unclassified drug
bacterial protein
ligand
silver
skin cream
transactivator protein
Article
biofilm
catalysis
chemotherapy
controlled study
cream
dispersion
drug screening
drug synthesis
epithelization
genotoxicity
HaCat cell line
human
human cell
hydrogel
in vivo study
keratinocyte
lipid peroxidation
mass spectrometry
molecular docking
molecular interaction
nonhuman
onion
spectroscopy
wound healing
wound healing assay
zone of inhibition
cell line
chemistry
conformation
cytology
disk diffusion
drug effect
immunology
metabolism
mutagen testing
physiology
M3 - Article
PY - 2018
SP - 41-55
ST - Silver complex of salicylic acid and its hydrogel-cream in wound healing
chemotherapy
TI - Silver complex of salicylic acid and its hydrogel-cream in wound healing
chemotherapy
85041401155&doi=10.1016%2fj.jinorgbio.2018.01.004&partnerID=40&md5=5c9ffba4958c4b2c
06e5885fa4670401
VL - 181
ID - 5551
ER -
TY - JOUR
AB - Background: There is increasing interest in the environmental and health
consequences of silver nanoparticles as the use of this material becomes
widespread. Although human exposure to nanosilver is increasing, only a few studies
address possible toxic effect of inhaled nanosilver. The objective of this study
was to determine whether very small commercially available nanosilver induces
pulmonary toxicity in mice following inhalation exposure.Results: In this study,
mice were exposed sub-acutely by inhalation to well-characterized nanosilver (3.3
mg/m3, 4 hours/day, 10 days, 5 ± 2 nm primary size). Toxicity was assessed by
enumeration of total and differential cells, determination of total protein,
lactate dehydrogenase activity and inflammatory cytokines in bronchoalveolar lavage
fluid. Lungs were evaluated for histopathologic changes and the presence of silver.
In contrast to published in vitro studies, minimal inflammatory response or
toxicity was found following exposure to nanosilver in our in vivo study. The
median retained dose of nanosilver in the lungs measured by inductively coupled
plasma - optical emission spectroscopy (ICP-OES) was 31 μg/g lung (dry weight)
immediately after the final exposure, 10 μg/g following exposure and a 3-wk rest
period and zero in sham-exposed controls. Dissolution studies showed that
nanosilver did not dissolve in solutions mimicking the intracellular or
extracellular milieu.Conclusions: Mice exposed to nanosilver showed minimal
pulmonary inflammation or cytotoxicity following sub-acute exposures. However,
longer term exposures with higher lung burdens of nanosilver are needed to ensure
that there are no chronic effects and to evaluate possible translocation to other
organs. © 2011 Stebounova et al; licensee BioMed Central Ltd.
AU - Stebounova, L. V.
AU - Adamcakova-Dodd, A.
AU - Kim, J. S.
AU - Park, H.
AU - O'Shaughnessy, P. T.
AU - Grassian, V. H.
AU - Thorne, P. S.
C7 - 5
DB - Scopus
DO - 10.1186/1743-8977-8-5
KW - Administration, Inhalation
Animals
Cytokines
Humans
Inflammation
Inhalation Exposure
Lung
Male
Mice
Mice, Inbred C57BL
Nanoparticles
Silver
X-Ray Diffraction
Murinae
Mus
cytokine
nanomaterial
nanosilver
silver
unclassified drug
nanoparticle
animal cell
animal experiment
animal model
animal tissue
article
cell function
controlled study
dissolution
enzyme activity
exposure
histopathology
in vitro study
in vivo study
lung lavage
lung toxicity
male
mouse
nonhuman
pneumonia
priority journal
sham procedure
toxic inhalation
animal
C57BL mouse
chemistry
cytology
drug effect
human
inflammation
lung
metabolism
pathology
X ray diffraction
M3 - Article
PY - 2011
ST - Nanosilver induces minimal lung toxicity or inflammation in a subacute murine
inhalation model
TI - Nanosilver induces minimal lung toxicity or inflammation in a subacute murine
inhalation model
78951469265&doi=10.1186%2f1743-8977-8-
5&partnerID=40&md5=05429767d06ec56104c110b502fee457
VL - 8
ID - 5749
ER -
TY - JOUR
AB - Purpose: Periodontal disease (PD), defined as oral inflammation caused by
dental plaque, is an emerging problem. PD may lead to tooth loss, and treatment
options are limited. In this study, we designed, synthesized, and characterized
silver nanoparticles (AgNPs) conjugated with chlorhexidine (AgNPs-CHL) or
metronidazole (AgNPs-PEG-MET) to determine whether they can be used to treat PDs.
Materials and Methods: AgNPs were synthesized and characterized by transmission
electron microscopy, UV-vis spectrometry, thermogravimetric analyses, and dynamic
light scattering. We determined the safety and the antimicrobial and anti-
inflammatory properties of synthesized AgNPs in an in vitro model of periodontitis.
Antimicrobial properties were determined by measuring the minimum inhibitory
concentration (MIC) and minimum biofilm eradication concentration (MBEC) on
reference strains of bacteria and fungi. Human gingival fibroblast (HGF-1), murine
macrophage (RAW264.7) and human foetal osteoblast (hFOB1.19) cells were used in the
study. Lipopolysaccharide (LPS) was used to induce inflammation. Cytokine levels
were measured using an enzyme-linked immunosorbent assay; metalloproteinase
expression was measured using Western blotting. Results: The synthesized AgNPs were
spherical and narrow-dispersed with an average diameter of 13.4 nm +/- 3.0 nm in
the case of AgNPs-CHL and 3.72 nm +/- 0.72 nm in the case of AgNPs-PEGMET. Both
types of AgNPs were active against bacteria and fungi. AgNPs-CHL proved to be a
more potent antimicrobial agent, although they were more cytotoxic than AgNPs-PEG-
MET; however, both demonstrated beneficial properties in nontoxic concentrations.
AgNPs-CHL and AgNPs-PEG-MET decreased the production of proinflammatory cytokines
IL-1 beta, IL-6, IL-8 and TNF alpha. Both agents also decreased the levels of
metalloproteinases MMP3 and MMP8, which may indicate that they will inhibit tissue
degradation. Conclusion: AgNPs-CHL and AgNPs-PEG-MET may be possible therapeutic
options for PD, as they have antibacterial and anti-inflammatory properties.
However, to fully understand the potential of AgNPs, our in vitro findings must be
evaluated in an in vivo model.
AN - WOS:000751830000001
AU - Steckiewicz, K. P.
AU - Cieciorski, P.
AU - Barcinska, E.
AU - Jaskiewicz, M.
AU - Narajczyk, M.
AU - Bauer, M.
AU - Kamysz, W.
AU - Megiel, E.
DO - 10.2147/IJN.S339046
PY - 2022
SN - 1178-2013
SP - 495-517
ST - Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery
Platforms: Their Potential Use in Treating Periodontitis
TI - Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery
Platforms: Their Potential Use in Treating Periodontitis
VL - 17
ID - 6145
ER -
TY - JOUR
AB - BM 13.177 (4-[2-(benzenesulfonamido)-ethyl]-phenoxyacetic acid) is a
representative of a new class of sulfonamidophenylcarboxylic acids which possess
platelet-inhibitory and anti-thrombotic activity and inhibits the contraction of
rabbit aorta stimulated by PG endoperoxides and TXA2. BM 13.177 S mg/kg body weight
p.o. protected rabbits from arachidonate-induced sudden death and ≥ 10 mg/kg dose-
dependently reduced the experimental thrombus formation induced in the rabbit aorta
by perivascular administration of silver nitrate. In guinea-pigs, the collagen-
induced bronchoconstriction was inhibited in a dose- and time-dependent fashion.
The formation of TXA2 and the TXA2-induced platelet aggregation and smooth muscle
contraction are probably crucial events in these experimental models. The
protective effect of BM 13.177 may, therefore, be due to the TXA2-antagonizing
effect of BM 13.177, which has been conclusively demonstrated in human platelets
(PATSCHEKE and STEGMEIER, Thrombosis Res., 33, 277-288 (1984).The antagonism of
TXA2 is supported by the observation that BM 13.177 also specifically inhibits the
contraction of isolated arterial strips from rabbits which were stimulated with the
thromboxane A2 mimetic U 46619. Schild-plot with a slope close to unity suggests a
competitive type of antagonism. BM 13.177 exhibited neither anti-inflammatory nor
ulcer-inducing activity of cyclooxygenase inhibitors. Furthermore it did not block
the TXB2 formation in spontaneously clotting blood from rabbits and did not inhibit
the release of prostacyclin-like activity from rabbit aortas. The lack of
toxicological effects in long-term toxicity studies in rat and dog, together with
the absence of objective and subjective side effects in the first human studies
have encouraged us to initiate clinical trials in order to evaluate the therapeutic
benefit of this new approach in humans. © 1984.
AU - Stegmeier, K.
AU - Pill, J.
AU - Müller-Beckmann, B.
AU - Schmidt, F. H.
AU - Witte, E. C.
AU - Wolff, H. P.
AU - Patscheke, H.
DB - Scopus
DO - 10.1016/0049-3848(84)90230-5
IS - 4
KW - antiplatelet drug
antithrombosis
BM 13.177
pharmacology
thromboxane antagonist
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Animals
Epoprostenol
Female
Fibrinolytic Agents
Guinea Pigs
Male
Muscle Contraction
Muscle, Smooth, Vascular
Platelet Aggregation
Prostaglandin Endoperoxides, Synthetic
Rabbits
Sulfonamides
Thrombosis
Thromboxane A2
Thromboxane B2
Thromboxanes
15 hydroxy 11alpha,9alpha epoxymethanoprosta 5,13 dienoic acid
adrenalin
arachidonic acid
indometacin
new drug
noradrenalin
prostacyclin
sulotroban
thromboxane A2
unclassified drug
animal experiment
aorta
article
blood and hemopoietic system
cardiovascular system
drug antagonism
drug comparison
drug efficacy
drug mechanism
drug sensitivity
great blood vessel
guinea pig
human
human cell
rabbit
thrombosis
M3 - Article
PY - 1984
SP - 379-395
ST - The pharmacological profile of the thromboxane A2 antagonist BM 13.177. A new
anti-platelet and anti-thrombotic drug
T2 - Thrombosis Research
TI - The pharmacological profile of the thromboxane A2 antagonist BM 13.177. A new
anti-platelet and anti-thrombotic drug
0021144201&doi=10.1016%2f0049-3848%2884%2990230-
5&partnerID=40&md5=661d9739f2fa0484a881245269da69be
VL - 35
ID - 5806
ER -
TY - JOUR
AB - Heterotopic ossification (HO) is defined as ectopic bone formation around
joints and in soft tissues following trauma, particularly blast-related extremity
injuries, thermal injuries, central nerve injuries, or orthopaedic surgeries,
leading to increased pain and diminished quality of life. Current treatment options
include pharmacotherapy with non-steroidal anti-inflammatory drugs, radiotherapy,
and surgical excision, but these treatments have limited efficacy and have
associated complication profiles. In contrast, small molecule inhibitors have been
shown to have higher specificity and less systemic cytotoxicity. Previous studies
have shown that bone morphogenetic protein (BMP) signaling and downstream non-
canonical (SMAD-independent) BMP signaling mediated induction of TGF-beta activated
kinase-1 (TAK1) contributes to HO. In the current study, small molecule inhibition
of TAK1, NG-25, was evaluated for its efficacy in limiting ectopic bone formation
following a rat blast associated lower limb trauma and a murine burn tenotomy
injury model. A significant decrease in total HO volume in the rat blast injury
model was observed by microCT imaging with no systemic complications following NG-
25 therapy. Furthermore, tissue-resident mesenchymal progenitor cells (MPCs)
harvested from rats treated with NG-25 demonstrated decreased proliferation,
limited osteogenic differentiation capacity, and reduced gene expression of Tac1,
Col10a1, Ibsp, Smad3, and Sox2 (P < 0.05). Single cell RNA-sequencing of murine
cells harvested from the injury site in a burn tenotomy injury model showed
increased expression of these genes in MPCs during stages of chondrogenic
differentiation. Additional in vitro cell cultures of murine tissue-resident MPCs
and osteochondrogenic progenitors (OCPs) treated with NG-25 demonstrated reduced
chondrogenic differentiation by 10.2-fold (P < 0.001) and 133.3-fold (P < 0.001),
respectively, as well as associated reduction in chondrogenic gene expression.
Induction of HO in Tak1 knockout mice demonstrated a 7.1-fold (P < 0.001) and 2.7-
fold reduction (P < 0.001) in chondrogenic differentiation of murine MPCs and OCPs,
respectively, with reduced chondrogenic gene expression. Together, our in vivo
models and in vitro cell culture studies demonstrate the importance of TAK1
signaling in chondrogenic differentiation and HO formation and suggest that small
molecule inhibition of TAK1 is a promising therapy to limit the formation and
progression of HO.
AN - WOS:000564722400004
AU - Strong, A. L.
AU - Spreadborough, P. J.
AU - Pagani, C. A.
AU - Haskins, R. M.
AU - Dey, D.
AU - Grimm, P. D.
AU - Kaneko, K.
AU - Marini, S.
AU - Huber, A. K.
AU - Hwang, C.
AU - Westover, K.
AU - Mishina, Y.
AU - Bradley, M. J.
AU - Levi, B.
AU - Davis, T. A.
C7 - 115517
DA - OCT
DO - 10.1016/j.bone.2020.115517
PY - 2020
SN - 8756-3282
1873-2763
ST - Small molecule inhibition of non-canonical (TAK1-mediated) BMP signaling
results in reduced chondrogenic ossification and heterotopic ossification in a rat
model of blast-associated combat-related lower limb trauma
T2 - BONE
TI - Small molecule inhibition of non-canonical (TAK1-mediated) BMP signaling
results in reduced chondrogenic ossification and heterotopic ossification in a rat
model of blast-associated combat-related lower limb trauma
VL - 139
ID - 6632
ER -
TY - CHAP
A2 - Saquib, Q.
A2 - Faisal, M.
A2 - AlKhedhairy, A. A.
A2 - Alatar, A. A.
AB - The potent antimicrobial properties of nanoparticulate silver (AgNPs) have
led to broad interest in using them in a wide range of commercial and medical
applications. Although numerous in vivo and in vitro studies have provided evidence
of toxic effects, rapid commercialization of AgNP-based nanomaterials has advanced
without characterization of their potential environmental and health hazards. There
is evidence that AgNPs can be translocated from the blood to the brain, regardless
the route of exposure, and accumulate in the brain over time. As the brain is
responsible for basic physiological functions and controls all human activities, it
is important to assess the hazardous influence of AgNPs released from widely used
nanoproducts and possible side effects of AgNP-based therapies. A number of studies
have suggested that the size, shape and surface coating, as well as rates of silver
ion release and interactions with proteins are the key factors determining the
neurotoxicity of AgNPs. AgNPs target endothelial cells forming the blood-brain
barrier, neurons and glial cells and leads finally to oxidative stress-related cell
death. In this chapter, we review in detail current data on the impact of AgNPs on
the central nervous system and discuss the possible mechanisms of their neurotoxic
effects.
AN - WOS:000443803700015
AU - Struzynska, L.
AU - Skalska, J.
DO - 10.1007/978-3-319-72041-8_14
10.1007/978-3-319-72041-8
PY - 2018
SN - 0065-2598
2214-8019
978-3-319-72041-8
978-3-319-72040-1
SP - 227-250
ST - Mechanisms Underlying Neurotoxicity of Silver Nanoparticles
T2 - CELLULAR AND MOLECULAR TOXICOLOGY OF NANOPARTICLES
TI - Mechanisms Underlying Neurotoxicity of Silver Nanoparticles
VL - 1048
ID - 6008
ER -
TY - JOUR
AB - Edible materials have attracted increasing attention because of their
excellent properties including availability, biocompatibility, biological activity,
and biodegradability. Natural polysaccharides, phenolic compounds, and proteins are
widely used in tissue regeneration. To better characterize their healing effect,
this review article describes the applications of edible materials in tissue
regeneration including wound healing and bone tissue regeneration. As an
introduction to the topic, their sources and main bioactive properties are
discussed. Then, the mechanism by which they facilitate wound healing based on
their hemostasis, antibacterial, anti-inflammatory, and antioxidant properties is
systematically investigated. Moreover, a more comprehensive discussion is presented
on the approaches by which edible materials can be used as scaffolds or agents for
the provision of the components of natural bones for regulating the level of
osteogenesis-related cytokines to enhance bone repair. Finally, the prospects of
edible materials for tissue regeneration are discussed. © 2021 Wiley-VCH GmbH
AU - Su, X.
AU - Xian, C.
AU - Gao, M.
AU - Liu, G.
AU - Wu, J.
C7 - 2100114
DB - Scopus
DO - 10.1002/mabi.202100114
IS - 8
KW - bone regeneration
edible materials
tissue engineering
wound healing
Bone and Bones
Bone Regeneration
Tissue Engineering
Tissue Scaffolds
Wound Healing
Bioactivity
Biocompatibility
Biodegradability
Tissue regeneration
alginic acid
chitin
chitosan
curcumin
cytokine
oxidized cellulose
phenol derivative
polysaccharide
protein
silver nanoparticle
biomaterial
Anti-inflammatories
Bioactive properties
Bone tissue regeneration
Natural bone
Natural polysaccharide
Phenolic compounds
Wound healing
bone development
bone remodeling
bone tissue
chronic wound
diabetic wound
hemostasis
human
nonhuman
pathophysiology
Review
skin injury
tissue regeneration
bone
tissue scaffold
Tissue
M3 - Review
PY - 2021
ST - Edible Materials in Tissue Regeneration
TI - Edible Materials in Tissue Regeneration
85107771820&doi=10.1002%2fmabi.202100114&partnerID=40&md5=b1896f364bec0ff54ff487802
6c08ec8
VL - 21
ID - 5254
ER -
TY - JOUR
AB - Myeloproliferative neoplasms (MPNs) are developing resistance to therapy by
JAK1/2 inhibitor ruxolitinib. To explore the mechanism of ruxolitinib's limited
effect, we examined the JAK1/2 mediated induction of proliferation related ERK1/2
and AKT signaling by proinflammatory interleukin-6 (IL-6) in MPN granulocytes and
JAK2V617F mutated human erythroleukemia (HEL) cells. We found that JAK1/2 or JAK2
inhibition prevented the IL-6 activation of STAT3 and AKT pathways in polycythemia
vera and HEL cells. Further, we showed that these inhibitors also blocked the IL-6
activation of the AKT pathway in primary myelofibrosis (PMF). Only JAK1/2 inhibitor
ruxolitinib largely activated ERK1/2 signaling in essential thrombocythemia and PMF
(up to 4.6 fold), with a more prominent activation in JAK2V617F positive
granulocytes. Regarding a cell cycle, we found that IL-6 reduction of HEL cells
percentage in G2M phase was reversed by ruxolitinib (2.6 fold). Moreover,
ruxolitinib potentiated apoptosis of PMF granulocytes (1.6 fold). Regarding DNA
replication, we found that ruxolitinib prevented the IL-6 augmentation of MPN
granulocytes frequency in the S phase of the cell cycle (up to 2.9 fold). The
inflammatory stimulation induces a cross-talk between the proliferation linked
pathways, where JAK1/2 inhibition is compensated by the activation of the ERK1/2
pathway during IL-6 stimulation of DNA replication.
AN - WOS:000456803100012
AU - Suboticki, T.
AU - Ajtic, O. M.
AU - Beleslin-Cokic, B. B.
AU - Bjelica, S.
AU - Djikic, D.
AU - Diklic, M.
AU - Lekovic, D.
AU - Gotic, M.
AU - Santibanez, J. F.
AU - Noguchi, C. T.
AU - Cokic, V. P.
DA - FEB
DO - 10.1002/cbin.11084
IS - 2
PY - 2019
SN - 1065-6995
1095-8355
SP - 192-206
ST - IL-6 stimulation of DNA replication is JAK1/2 mediated in cross-talk with
hyperactivated ERK1/2 signaling
T2 - CELL BIOLOGY INTERNATIONAL
TI - IL-6 stimulation of DNA replication is JAK1/2 mediated in cross-talk with
hyperactivated ERK1/2 signaling
VL - 43
ID - 6714
ER -
TY - JOUR
AB - Platinum nanoparticles (Pt NPs) was synthesized via a facile and cost
competitive ont-pot green mediated synthesis using cell free cultural filtrate
(microgravity simulated grown Penicillium chrysogenum) as a reducing agent. The
toxicity effect of synthesized Pt NPs toward myoblast C2C12 carcinoma cells was
then investigated. The particle size analyzer (DLS) and transmission electron
microscopy (TEM) results demonstrates that both NG-Pt NPs and MG-Pt NPS are
spherical in shape with an average diameter of 15 nm and 8.5 nm, respectively. The
results from UV-visible (UV-vis) spectroscopy and X-ray diffraction (XRD) analysis
show a characteristic strong resonance centered at 265 nm and a single crystalline
nature, respectively. The results derived from in vitro cytotoxicity showed a
significant concentration-dependent decrement in cell viability when C2C12 cells
were exposed to Pt NPs. Such decrement in cell viability is because of increased
reactive oxygen species (ROS) generation. Cell apoptosis was proved by acridine
orange-ethidium bromide (AO/EtBr) dual staining, annexin V-FITC/PI-staining and
immunocytochemistry. Moreover, the protein expression of both (i) apoptosis related
proteins such as cas-3 and cas-9, (ii) inflammatory response proteins such as TNF-
alpha, TGF-beta and NF-kappa B were significantly upregulated in MG-Pt NPs treated
cells than NG-Pt NPs treated cells. Uptake and intracellular localization of MG-Pt
NPs caused by accumulation of autophagosomes in C2C12 cells and bacterial cells,
indicate that synthesized MG-Pt NPs enable for the swift cell apoptosis than NG-Pt
NPs. Interestingly, At the concentration of 40 and 80 mu g/ml MG-Pt NPs showed more
potent cytotoxicity toward cancer cells while, under identical concentration, NG-Pt
NPs exhibited rather lower cytotoxicity. Overall, our results demonstrated that MG-
Pt NPs could be selectively inhibit the growth of cancer cells via ROS-mediated
nucleus NF-kappa B and caspases activation when compared to NG-Pt NPs.
AN - WOS:000426040800009
AU - Subramaniyan, S. A.
AU - Sheet, S.
AU - Vinothkannan, M.
AU - Yoo, D. J.
AU - Lee, Y. S.
AU - Belal, S. A.
AU - Shim, K. S.
DA - MAY
DO - 10.1166/jnn.2018.14661
IS - 5
PY - 2018
SN - 1533-4880
1533-4899
SP - 3110-3125
ST - One-Pot Facile Synthesis of Pt Nanoparticles Using Cultural Filtrate of
Microgravity Simulated Grown P. chrysogenum and Their Activity on Bacteria and
Cancer Cells
TI - One-Pot Facile Synthesis of Pt Nanoparticles Using Cultural Filtrate of
Microgravity Simulated Grown P. chrysogenum and Their Activity on Bacteria and
Cancer Cells
VL - 18
ID - 6420
ER -
TY - JOUR
AB - Silver nanoparticles (GO@AgNPs) were biogenically produced using Methanolic
Extract of Bark (MEB) of the plant Grewia orbiculata. Initially GO@AgNPs were
synthesised and characterised using UV-spectrometer. The physicochemical stability
was determined to be robust and unaltered in terms of pH, temperature, and salinity
changes. GO@AgNPs were also evaluated for their biological potential. The
nanoparticles displayed strong radical scavenging capacities in the 2,2-
diphenylpicrylhydrazyl (DPPH) and 2,2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic
Acid (ABTS) assays, with IC50 values of 4.5 µg/mL and 3.5 µg/mL, respectively. The
Ferric Reducing Antioxidant Power Assay (FRAP) assay revealed that it has
outstanding metal chelating activity, with a value of 6 µg/mL. The GO@AgNPs were
found to have excellent and enhanced antimicrobial potency than the crude extract
against 10 human pathogens tested, namely Staphylococcus aureus, Methicillin
resistant Staphylococcus aureus, Streptococcus epidermidis, Enterococcus faecalis,
Enterococcus faecium, Escherichia coli, Acinetobacter baumannii, Pseudomonas
aeruginosa, and Mycobacterium smegmatis. It was also reported to demonstrate
remarkable anti-cancerous property against A549 lung carcinoma cells at a low
dosage of 46 µg/mL, while being non-toxic to normal cell lines such as tracheal
epithelial cells even at highest conc. of 320 µg/mL tested to assess the
cytotoxicity. They were proven to be biocompatible with plasma, as GO@AgNPs
exhibited no substantial agglutination of RBCs up to a concentration of 50 mg/mL,
and in vitro anti-inflammatory effect of GO@AgNPs revealed protection against BSA
denaturation up to a concentration of 100 mg/mL. These results indicate that
GO@AgNPs might be employed in biological and pharmacological applications. © 2023,
Indian journals. All rights reserved.
AU - Suguna, M.
AU - Umesha, S.
DB - Scopus
DO - 10.5958/0975-6892.2023.00020.5
IS - 1
KW - anti-cancerous activity
Antioxidant property
cytotoxicity
in vitro anti-inflammatory activity
2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid)
antioxidant
chlorhexidine
diclofenac
flavonoid
grewia orbiculata extract
plant extract
polyphenol
quercetin
silver nanoparticle
tannin
unclassified drug
Acinetobacter
Acinetobacter baumannii
antifungal activity
antigen antibody reaction
Article
assay
bacterium isolation
biocompatibility
biofilm
Candida albicans
cell growth
cell proliferation
cell viability
chelation
controlled study
cytotoxicity assay
denaturation
drug synthesis
Enterococcus faecium
epithelium cell
Escherichia coli
Ganoderma lucidum
human
human cell
IC50
in vitro study
low drug dose
lung carcinoma
MTT assay
Mycobacterium smegmatis
nanomedicine
nonhuman
pH
phytochemistry
salinity
M3 - Article
PY - 2023
SP - 202-214
ST - Biogenic synthesis of silver nanoparticles with Grewia orbiculata (GO@AgNPs)
and evaluation of their bioactive potential
T2 - Medicinal Plants
TI - Biogenic synthesis of silver nanoparticles with Grewia orbiculata (GO@AgNPs)
and evaluation of their bioactive potential
85153537127&doi=10.5958%2f0975-
6892.2023.00020.5&partnerID=40&md5=dc222c02ad84a4b76015ac081a99ce11
VL - 15
ID - 4982
ER -
TY - JOUR
AB - In the current research, we decided to formulate gold nanoparticles in
aqueous medium using plant leaf aqueous extract as a new therapeutic drug for
determining the local anesthetic, anti-inflammatory and analgesic activities in the
in vivo condition. The common chemical tests i.e., UV-Visible Spectroscopy (UV-
Vis), Transmission Electron Microscopy (TEM), and Fourier Transformed Infrared
Spectroscopy (FT-IR) were used for characterizing of nanoparticles. To survey the
cytotoxicity properties of nanoparticles, MTT assay was used on the human normal
cell lines. Analgesic activity was tested in mice using various doses orally.
Acetic acid-induced writhing episodes were significantly and dose-dependently
reduced. The local anesthetic activity were tested in frog and guinea pig models,
and it was seen that in both animals, the nanoparticle produces significant local
anesthetic activity. At the same dose, its anti-inflammatory activity was also
tested. Carrageenin-induced paw edema in mice was significantly reduced after oral
administration. It seems the recent nanoparticle may be use as a novel therapeutic
drug with local anesthetic, anti-inflammatory and analgesic activities in future.
AN - WOS:000860119500001
AU - Sui, J. L.
AU - Jin, M. X.
AU - Morovvati, H.
AU - Goorani, S.
C6 - AUG 2022
C7 - 109642
DA - SEP
DO - 10.1016/j.inoche.2022.109642
PY - 2022
SN - 1387-7003
1879-0259
ST - Local anesthetic, anti-inflammatory and analgesic activities of nanoparticles
green-formulated by plant extract
T2 - INORGANIC CHEMISTRY COMMUNICATIONS
TI - Local anesthetic, anti-inflammatory and analgesic activities of nanoparticles
green-formulated by plant extract
VL - 143
ID - 6022
ER -
TY - JOUR
AB - Uvarianarum has anticancer, anti-inflammatory, antifungal and anti-HIV
properties which are effective in action. The aim of the study was to biosynthesize
silver nanoparticles using Uvarianarum leaves extract and determine their
anticancer activity against MCF-7 breast cancer cell line. Silver nanoparticles has
become an important prioritized nanomaterial among the others as they are non-toxic
to the cells exhibiting as a defensive inorganic antimicrobial agent. Presence of
AgNPs confirmed with yellowish-brown colour at 8:2 ratio. The synthesized
nanoparticles were characterized through SEM analysis along with Fluorescence
microscope analysis.TheEtBr/Ao staining results suggest that AgNPs may exert its
antiproliferative effect on MCF-7 cell line by suppressing its growth and inducing
apoptosis.The cytotoxic activity studied by MTT assay in MCF-7 cell lines revealed
that the U. narumAgNPs were non-toxic exhibiting 50% growth inhibition as 22.19 mu
g(/)ml.The outcomes revealed that the U.narumAgNPs as the most active, in terms of
IC50, with a more pronounced efficacy against breast cancer cell lines.
AN - WOS:000778730200023
AU - Sujithra, M.
AU - Sathya, R.
AU - NirmalaDevi, P.
AU - Prema, P.
DA - DEC 20
PY - 2021
SN - 2250-0480
SP - 138-143
ST - BIOSYNTHESIS AND CHARACTERISATION OF SILVER NANOPARTICLES FROM UVARIANARUM
EXTRACT
T2 - INTERNATIONAL JOURNAL OF LIFE SCIENCE AND PHARMA RESEARCH
TI - BIOSYNTHESIS AND CHARACTERISATION OF SILVER NANOPARTICLES FROM UVARIANARUM
EXTRACT
ID - 5882
ER -
TY - JOUR
AB - Silver nanoparticles are increasingly used in various products, due to their
antibacterial properties. Despite its wide spread use, only little information on
possible adverse health effects exists. Therefore, the aim of this study was to
assess the toxic potential of silver nanoparticles (<100 nm) in human lung
epithelial (A549) cells and the underlying mechanism of its cellular toxicity.
Silver nanoparticles induced dose and time-dependent cytotoxicity in A549 cells
demonstrated by MTT and LDH assays. Silver nanoparticles were also found to induce
oxidative stress in dose and time-dependent manner indicated by depletion of GSH
and induction of ROS, LPO, SOD, and catalase. Further, the activities of caspases
and the level of proinflammatory cytokines, namely interleukin-1β (IL-1β) and
interleukin-6 (IL-6) were significantly higher in treated cells. DNA damage, as
measured by single cell gel electrophoresis, was also dose and time-dependent
signicants in A549 cells. This study investigating the effects of silver
nanoparticles in human lung epithelial cells has provided valuable insights into
the mechanism of potential toxicity induced by silver nanoparticles and warrants
more careful assessment of silver nanoparticles before their industrial
applications. © 2013 Wiley Periodicals, Inc.
AU - Suliman Y, A. O.
AU - Ali, D.
AU - Alarifi, S.
AU - Harrath, A. H.
AU - Mansour, L.
AU - Alwasel, S. H.
DB - Scopus
DO - 10.1002/tox.21880
IS - 2
KW - Cyto-genotoxicity
Human lung epithelial cells
Oxidative stress
Proinflammatory cytokines
Apoptosis
Biological Markers
Cell Line
Cell Survival
Epithelial Cells
Humans
Inflammation
Lipid Peroxidation
Metal Nanoparticles
Mutagenicity Tests
Mutagens
Oxidative Stress
Respiratory Mucosa
Silver
Biological organs
Cell culture
Cells
Electrophoresis
Metal nanoparticles
Respiratory system
Toxicity
acetylcysteine
alpha tocopherol
ascorbic acid
caspase 3
caspase 9
glutathione
interleukin 1beta
interleukin 6
silver nanoparticle
biological marker
metal nanoparticle
mutagenic agent
silver
Adverse health effects
Cellular toxicities
Genotoxic effects
Genotoxicities
Human lung
Single cell gel electrophoresis
A549 cell line
apoptosis
Article
cell damage
cell density
cell structure
cell viability
comet assay
controlled study
cytotoxicity
DNA damage
DNA strand breakage
genotoxicity
human
human cell
inflammation
lipid peroxidation
mitochondrion
oxidative stress
priority journal
protein expression
zeta potential
cell line
cell survival
chemically induced
cytology
drug effects
epithelium cell
metabolism
mutagen testing
pathology
respiratory tract mucosa
M3 - Article
PY - 2015
SP - 149-160
ST - Evaluation of cytotoxic, oxidative stress, proinflammatory and genotoxic
effect of silver nanoparticles in human lung epithelial cells
TI - Evaluation of cytotoxic, oxidative stress, proinflammatory and genotoxic
effect of silver nanoparticles in human lung epithelial cells
84921612967&doi=10.1002%2ftox.21880&partnerID=40&md5=34365b570f09a096930613e2ee5aa4
8d
VL - 30
ID - 5589
ER -
TY - JOUR
AB - Nanotechnology is the study, production and controlled manipulation of
materials with a grain size < 100 nm. At this level, the laws of classical
mechanics fall away and those of quantum mechanics take over, resulting in unique
behaviour of matter in terms of melting point, conductivity and reactivity.
Additionally, and likely more significant, as grain size decreases, the ratio of
surface area to volume drastically increases, allowing for greater interaction
between implants and the surrounding cellular environment. This favourable increase
in surface area plays an important role in mesenchymal cell differentiation and
ultimately bone-implant interactions. Basic science and translational research have
revealed important potential applications for nanotechnology in orthopaedic
surgery, particularly with regard to improving the interaction between implants and
host bone. Nanophase materials more closely match the architecture of native
trabecular bone, thereby greatly improving the osseo-integration of orthopaedic
implants. Nanophase-coated prostheses can also reduce bacterial adhesion more than
conventionally surfaced prostheses. Nanophase selenium has shown great promise when
used for tumour reconstructions, as has nanophase silver in the management of
traumatic wounds. Nanophase silver may significantly improve healing of peripheral
nerve injuries, and nanophase gold has powerful anti-inflammatory effects on tendon
inflammation. Considerable advances must be made in our understanding of the
potential health risks of production, implantation and wear patterns of nanophase
devices before they are approved for clinical use. Their potential, however, is
considerable, and is likely to benefit us all in the future. ©2014 The British
Editorial Society of Bone & Joint Surgery.
AU - Sullivan, M. P.
AU - McHale, K. J.
AU - Parvizi, J.
AU - Mehta, S.
DB - Scopus
DO - 10.1302/0301-620X.96B5.33606
IS - 5
KW - Biomedical Research
Bone Substitutes
Humans
Nanomedicine
Orthopedic Procedures
Tissue Scaffolds
Drug delivery
Hydroxyapatite
Nano-orthopaedics
Nanophase
Nanotechnology
Osseo-integration
cefazolin
chromium
cobalt
fibronectin
gold nanoparticle
hydroxyapatite
molybdenum
nanocomposite
nanocrystal
nanofilm
selenium
silver nanoparticle
titanium
vitronectin
tissue scaffold
bacterium adherence
bone cell
cell surface
chemical reaction
conductance
cytotoxicity
device safety
endoprosthesis
fracture healing
health hazard
human
implant
inflammation
melting point
mesenchymal stroma cell
mesenchyme cell
molecular interaction
nanoengineering
nanotechnology
nanotoxicology
nerve regeneration
orthopedic implant
orthopedic prosthesis
orthopedic surgery
osteoblast
oxidative stress
particle size
peripheral nerve injury
postoperative infection
priority journal
prosthesis loosening
quantum mechanics
review
surface property
tendon disease
trabecular bone
translational research
wound healing
bone prosthesis
equipment
medical research
methodology
nanomedicine
M3 - Review
PY - 2014
SP - 569-573
ST - Current concepts in orthopaedic surgery and future directions
T2 - Bone and Joint Journal
TI - Current concepts in orthopaedic surgery and future directions
84900531049&doi=10.1302%2f0301-
620X.96B5.33606&partnerID=40&md5=268204b8a2825cd4f5ce6011baab994a
VL - 96 B
ID - 5612
ER -
TY - JOUR
AB - Silica nanoparticles (SiNPs), one of the most produced nanoparticles (NPs) in
the world, are used in all aspects of life. The increased application of SiNPs,
especially in medicine, has raised considerable concern regarding their
toxicological impact. Previous studies have shown that SiNPs can pass through the
reproductive barrier and cause reproductive organ dysfunction by destroying Sertoli
cells, Leydig cells, and germ cells. However, little is known about the mechanism
of SiNPs-induced reproductive toxicity. In the present study, 5-week-old male mice
were intraperitoneally administered SiNPs per day for 1 week at a dose of 0.2 mg
per mouse. The results showed that SiNPs could cause damage to the structure of the
testis and the epididymis and change the reproductive organ coefficients, leading
to decreases of 56.1% and 55.3% in the rates of sperm concentration and motility
and an increase of 168.8% in the rate of sperm abnormality. Moreover, the serum
testosterone level obviously decreased from 18.77 to 5.23 mu g/ml after exposure,
and the transcription statuses of some key genes involved in the synthesis and
transport of testosterone in the testis were also affected. Additional experiments
showed that SiNPs exposure during puberty induced oxidative stress and an
inflammatory response, as shown by the changed activity of superoxide dismutase
(SOD), increased contents of malondialdehyde (MDA), and excess expression of
proinflammatory factors, including TNF-alpha and IL-1 beta. Furthermore, the
administration of SiNPs caused DNA damage and cell apoptosis, which were presented
by the increased apoptotic cells in the sections of testis and epididymis and
activation of the TNF-alpha/TNFR I-mediated pro-apoptotic pathway. In conclusion,
these results indicate that SiNPs exposure during puberty significantly damaged the
structure and function of the testis and epididymis by inducing oxidative stress
and cell apoptosis. This study provides novel insight into SiNPs-induced
reproductive toxicity during puberty, which warrants a more careful assessment of
SiNPs before their application in juvenile supplies.
AN - WOS:000745504800004
AU - Sun, F. L.
AU - Wang, X. Y.
AU - Zhang, P. Z.
AU - Chen, Z. Y.
AU - Guo, Z. Y.
AU - Shang, X.
C6 - JAN 2022
DA - MAY
DO - 10.1007/s11356-021-18215-6
IS - 24
PY - 2022
SN - 0944-1344
1614-7499
SP - 36640-36654
ST - Reproductive toxicity investigation of silica nanoparticles in male pubertal
mice
TI - Reproductive toxicity investigation of silica nanoparticles in male pubertal
mice
VL - 29
ID - 6286
ER -
TY - JOUR
AB - The widespread use of silver nanoparticles (AgNPs), their many sources for
human exposure, and the ability of AgNPs to enter organisms and induce general
toxicological responses have raised concerns regarding their public health and
environmental safety. To elucidate the differential toxic effects of
polyvinylpyrrolidone-capped AgNPs with different primary particle sizes (i.e. 5,
50, and 75 nm), we performed a battery of cytotoxicity and genotoxicity assays and
examined the inflammatory responses in two human cell lines (i.e. HepG2 and A549).
Concentration-dependent decreases in cell proliferation and mitochondrial membrane
potential and increases in cytokine (i.e. interleukin-6 and interleukin-8)
excretion indicated disruption of mitochondrial function and inflammation as the
main mediating factors of AgNPs-induced cytotoxicity. An incremental increase in
genotoxicity with decreasing AgNPs diameter was noted in HepG2 cells, which was
associated with S and G2/M accumulation and transcriptional activation of the
GADD45 alpha promoter as reflected by luciferase activity. Dose-related genetic
damage, as indicated by Olive tail moment and micronucleus formation, was also
observed in A549 cells, but these effects as well as the AgNPs-induced cytotoxicity
were more associated with ionic Ag release from nanoparticles (NPs). In summary,
the present study addressed different toxicity mechanisms of AgNPs, depending on
the cell model, toxicological endpoint, particle size, and degree of Ag+ release
from NPs. The results suggest that the GADD45 alpha promoter-driven luciferase
reporter cell system provided a rapid screening tool for the identification of
genotoxic properties of NPs across a range of different sizes and concentrations.
AN - WOS:000626671500001
AU - Sun, J. J.
AU - Wan, J. M.
AU - Zhai, X. D.
AU - Wang, J. S.
AU - Liu, Z. Y.
AU - Tian, H. L.
AU - Xin, L. L.
C6 - FEB 2021
C7 - 0748233721996561
DA - APR
DO - 10.1177/0748233721996561
IS - 4
PY - 2021
SN - 0748-2337
1477-0393
SP - 198-209
ST - Silver nanoparticles: Correlating particle size and ionic Ag release with
cytotoxicity, genotoxicity, and inflammatory responses in human cell lines
TI - Silver nanoparticles: Correlating particle size and ionic Ag release with
VL - 37
ID - 5845
ER -
TY - JOUR
AB - The widespread use of silver nanoparticles (AgNPs), their many sources for
human exposure, and the ability of AgNPs to enter organisms and induce general
toxicological responses have raised concerns regarding their public health and
environmental safety. To elucidate the differential toxic effects of
polyvinylpyrrolidone-capped AgNPs with different primary particle sizes (i.e. 5,
50, and 75 nm), we performed a battery of cytotoxicity and genotoxicity assays and
examined the inflammatory responses in two human cell lines (i.e. HepG2 and A549).
Concentration-dependent decreases in cell proliferation and mitochondrial membrane
potential and increases in cytokine (i.e. interleukin-6 and interleukin-8)
excretion indicated disruption of mitochondrial function and inflammation as the
main mediating factors of AgNPs-induced cytotoxicity. An incremental increase in
genotoxicity with decreasing AgNPs diameter was noted in HepG2 cells, which was
associated with S and G2/M accumulation and transcriptional activation of the
GADD45α promoter as reflected by luciferase activity. Dose-related genetic damage,
as indicated by Olive tail moment and micronucleus formation, was also observed in
A549 cells, but these effects as well as the AgNPs-induced cytotoxicity were more
associated with ionic Ag release from nanoparticles (NPs). In summary, the present
study addressed different toxicity mechanisms of AgNPs, depending on the cell
model, toxicological endpoint, particle size, and degree of Ag+ release from NPs.
The results suggest that the GADD45α promoter-driven luciferase reporter cell
system provided a rapid screening tool for the identification of genotoxic
properties of NPs across a range of different sizes and concentrations. © The
Author(s) 2021.
AU - Sun, J.
AU - Wan, J.
AU - Zhai, X.
AU - Wang, J.
AU - Liu, Z.
AU - Tian, H.
AU - Xin, L.
DB - Scopus
DO - 10.1177/0748233721996561
IS - 4
KW - cytotoxicity
GADD45α
genotoxicity
inflammation
luciferase reporter gene
A549 Cells
Cell Line
Comet Assay
Cytotoxins
Hep G2 Cells
Humans
Inflammation
Intracellular Signaling Peptides and Proteins
Luciferases
Metal Nanoparticles
Mutagens
Particle Size
Povidone
Silver
interleukin 6
interleukin 8
luciferase
povidone
silver
silver nanoparticle
cytotoxin
metal nanoparticle
mutagenic agent
signal peptide
A-549 cell line
Article
cell cycle G2 phase
cell cycle M phase
cell cycle S phase
cell proliferation
controlled study
GADD45 alpha gene
gene
genetic damage
Hep-G2 cell line
human
human cell
micronucleus
Olive tail moment
particle size
promoter region
reporter gene
transcription initiation
cell line
comet assay
dose response
M3 - Article
PY - 2021
SP - 198-209
ST - Silver nanoparticles: Correlating particle size and ionic Ag release with
TI - Silver nanoparticles: Correlating particle size and ionic Ag release with
85101624390&doi=10.1177%2f0748233721996561&partnerID=40&md5=1be9cc70cd013bf955ad0c7
3c65a4801
VL - 37
ID - 5242
ER -
TY - JOUR
AB - Dengue virus (DENV) is a mosquito-transmitted single stranded RNA virus
belonging to genus Flavivirus. The virus is endemic in the tropical and subtropical
countries of the world, causing diseases classified according to symptoms and
severity (from mild to severe) as dengue fever, dengue hemorrhagic fever, and
dengue shock syndrome. Among a variety of human cell types targeted by DENV,
monocytes, macrophages, and dendritic cells are members of innate immunity, capable
of mounting rapid inflammatory responses. These cells are also major antigen
presenting cells, responsible for activating the adaptive immunity for long-term
memory. This paper is an overview of the current understanding of the following
mutually affected aspects: DENV structure, viral infectivity, cellular receptors,
innate immune response, and adaptive immunity.
AN - WOS:000315275600001
AU - Sun, P. F.
AU - Kochel, T. J.
C7 - 843469
DO - 10.1155/2013/843469
PY - 2013
SN - 1537-744X
ST - The Battle between Infection and Host Immune Responses of Dengue Virus and
Its Implication in Dengue Disease Pathogenesis
T2 - SCIENTIFIC WORLD JOURNAL
TI - The Battle between Infection and Host Immune Responses of Dengue Virus and
Its Implication in Dengue Disease Pathogenesis
ID - 6721
ER -
TY - JOUR
AB - Gold nanoparticles (AuNPs) have wide applications in the field of diagnosis
and treatment of diseases which are attributed to their compatibility and high
efficiency of drug delivery, because of their eco-friendly nature and easy
handling. In the current study, we have used an extract of Vitex negundo, a
traditional anti-inflammatory folk medicine in India and synthesized gold
nanoparticles (VnAuNPs), to know the effects of anti-bacterial, antioxidant, and
anti-inflammatory properties both in vivo and in vitro. V. negundo leaf extracts
(VnLE) per se yielded high flavonoids, saponins, tannins, alkaloids, glycosides,
phenols, terpenoids. The synthesized VnAuNPs in leaf extracts was confirmed by UV-
vis spectra at 543 nm and further by Fourier transform infrared spectroscopy
(FTIR), Scanning electron microscope (SEM) and through Dynamic light scattering
(DLS). The FTIR showed the peaks at 3345, 1638, 692, 683, 662 cm(-1) which are
responsible for the reduction and capping of gold nanoparticles. The size and shape
of the AuNPs were determined by SEM. DLS study analysed the particle size
distribution of AuNPs. The VnAuNPs showed significant antibacterial activity on
both gram positive and negative bacteria. VnAuNPs also showed strong antioxidant
(DPPH, H2O2 scavenging, Nitric oxide scavenging power and reducing power)
activities when compared to VnLE extract. The VnAuNPs exhibited strong anti-
inflammatory activities (COX-2, lipoxygenase and xanthine oxidase inhibitory
activity) in in vitro (HeLa cell model) and in vivo study of carrageenan induced
paw edema model in Swiss albino mice (paw edema and acetic acid-induced writhing
test). Results suggested that the synthesized VnAuNPs are potential candidates for
treating inflammatory diseases and may find application in clinical studies.
AN - WOS:000511990800017
AU - Sunayana, N.
AU - Uzma, M.
AU - Dhanwini, R. P.
AU - Govindappa, M.
AU - Prakash, H. S.
AU - Raghavendra, B. V.
DA - MAR
DO - 10.1007/s10876-019-01661-1
IS - 2
PY - 2020
SN - 1040-7278
1572-8862
SP - 463-477
ST - Green Synthesis of Gold Nanoparticles from Vitex negundo Leaf Extract to
Inhibit Lipopolysaccharide-Induced Inflammation Through In Vitro and In Vivo
TI - Green Synthesis of Gold Nanoparticles from Vitex negundo Leaf Extract to
Inhibit Lipopolysaccharide-Induced Inflammation Through In Vitro and In Vivo
VL - 31
ID - 6306
ER -
TY - JOUR
AB - Background: Gold nanoparticles are widely used in consumer products,
including cosmetics, food packaging, beverages, toothpaste, automobiles, and
lubricants. With this increase in consumer products containing gold nanoparticles,
the potential for worker exposure to gold nanoparticles will also increase. Only a
few studies have produced data on the in vivo toxicology of gold nanoparticles,
meaning that the absorption, distribution, metabolism, and excretion (ADME) of gold
nanoparticles remain unclear. Results: The toxicity of gold nanoparticles was
studied in Sprague Dawley rats by inhalation. Seven-week-old rats, weighing
approximately 200 g (males) and 145 g (females), were divided into 4 groups (10
rats in each group): fresh-air control, low-dose (2.36 x 10(4) particle/cm(3), 0.04
mu g/m(3)), middle-dose (2.36 x 10(5) particle/cm(3), 0.38 mu g/m(3)), and high-
dose (1.85 x 10(6) particle/cm(3), 20.02 mu g/m(3)). The animals were exposed to
gold nanoparticles (average diameter 4-5 nm) for 6 hours/day, 5 days/week, for 90-
days in a whole-body inhalation chamber. In addition to mortality and clinical
observations, body weight, food consumption, and lung function were recorded
weekly. At the end of the study, the rats were subjected to a full necropsy, blood
samples were collected for hematology and clinical chemistry tests, and organ
weights were measured. Cellular differential counts and cytotoxicity measurements,
such as albumin, lactate dehydrogenase (LDH), and total protein were also monitored
in a cellular bronchoalveolar lavage (BAL) fluid. Among lung function test
measurements, tidal volume and minute volume showed a tendency to decrease
comparing control and dose groups during the 90-days of exposure. Although no
statistically significant differences were found in cellular differential counts,
histopathologic examination showed minimal alveoli, an inflammatory infiltrate with
a mixed cell type, and increased macrophages in the high-dose rats. Tissue
distribution of gold nanoparticles showed a dose-dependent accumulation of gold in
only lungs and kidneys with a gender-related difference in gold nanoparticles
content in kidneys. Conclusions: Lungs were the only organ in which there were
dose-related changes in both male and female rats. Changes observed in lung
histopathology and function in high-dose animals indicate that the highest
concentration (20 mu g/m(3)) is a LOAEL and the middle concentration (0.38 mu
g/m(3)) is a NOAEL for this study.
AN - WOS:000292206900001
AU - Sung, J. H.
AU - Ji, J. H.
AU - Park, J. D.
AU - Song, M. Y.
AU - Song, K. S.
AU - Ryu, H. R.
AU - Yoon, J. U.
AU - Jeon, K. S.
AU - Jeong, J.
AU - Han, B. S.
AU - Chung, Y. H.
AU - Chang, H. K.
AU - Lee, J. H.
AU - Kim, D. W.
AU - Kelman, B. J.
AU - Yu, I. J.
C7 - 16
DA - MAY 14
DO - 10.1186/1743-8977-8-16
PY - 2011
SN - 1743-8977
ST - Subchronic inhalation toxicity of gold nanoparticles
TI - Subchronic inhalation toxicity of gold nanoparticles
VL - 8
ID - 6290
ER -
TY - JOUR
AB - The subchronic inhalation toxicity of silver nanoparticles was studied in
Sprague-Dawley rats. Eight-week-old rats, weighing approximately 253.2 g (males)
and 162.6 g (females), were divided into four groups (10 rats in each group):
fresh-air control, low dose (0.6 × 106 particle/cm3, 49 μg/m3), middle dose (1.4 ×
106 particle/cm3, 133 μg/ m3), and high dose (3.0 × 106 particle/ cm3, 515 μg/m3).
The animals were exposed to silver nanoparticles (average diameter 18-19 nm) for 6
h/day, 5 days/ week, for 13 weeks in a whole-body inhalation chamber. In addition
to mortality and clinical observations, body weight, food consumption, and
pulmonary function tests were recorded weekly. At the end of the study, the rats
were subjected to a full necropsy, blood samples were collected for hematology and
clinical chemistry tests, and the organ weights were measured. Bile-duct
hyperplasia in the liver increased dose dependently in both the male and female
rats. Histopathological examinations indicated dose-dependent increases in lesions
related to silver nanoparticle exposure, including mixed inflammatory cell
infiltrate, chronic alveolar inflammation, and small granulomatous lesions. Target
organs for silver nanoparticles were considered to be the lungs and liver in the
male and female rats. No observable adverse effect level of 100 μg/m3 is suggested
from the experiments. © The Author 2008. Published by Oxford University Press on
behalf of the Society of Toxicology. All rights reserved.
AU - Sung, J. H.
AU - Ji, J. H.
AU - Park, J. D.
AU - Yoon, J. U.
AU - Kim, D. S.
AU - Jeon, K. S.
AU - Song, M. Y.
AU - Jeong, J.
AU - Han, B. S.
AU - Han, J. H.
AU - Chung, Y. H.
AU - Chang, H. K.
AU - Lee, J. H.
AU - Cho, M. H.
AU - Kelman, B. J.
AU - Yu, I. J.
DB - Scopus
DO - 10.1093/toxsci/kfn246
IS - 2
KW - Lung
Nanoparticles
Animals
Blood Chemical Analysis
Body Weight
Eating
Erythrocyte Aggregation
Female
Inhalation Exposure
Kidney Function Tests
Lung Diseases
Male
Organ Size
Particle Size
Pneumonia
Rats
Sex Characteristics
Silver
Animalia
Rattus
metal nanoparticle
silver nanoparticle
unclassified drug
adrenal gland
animal experiment
animal model
animal tissue
article
autopsy
bile duct hyperplasia
blood chemistry
blood sampling
body weight
brain
controlled study
female
food intake
heart
histopathology
hyperplasia
kidney
liver toxicity
lung function test
lung toxicity
male
mortality
nonhuman
olfactory bulb
organ weight
ovary
rat
sex difference
Sprague Dawley rat
target organ
testis
thymus
tissue distribution
toxic inhalation
toxicity testing
M3 - Article
PY - 2009
SP - 452-461
ST - Subchronic inhalation toxicity of silver nanoparticles
TI - Subchronic inhalation toxicity of silver nanoparticles
64349090027&doi=10.1093%2ftoxsci
%2fkfn246&partnerID=40&md5=8f5feb887f29dccd99d1f69be6667f37
VL - 108
ID - 5772
ER -
TY - JOUR
AB - The antimicrobial activity of silver nanoparticles has resulted in their
widespread use in many consumer products. However, despite the continuing increase
in the population exposed to silver nanoparticles, the effects of prolonged
exposure to silver nanoparticles have not been thoroughly determined. Accordingly,
this study attempted to investigate the inflammatory responses and pulmonary
function changes in rats during 90 days of inhalation exposure to silver
nanoparticles. The rats were exposed to silver nanoparticles (18 nm diameter) at
concentrations of 0.7 x 10(6) particles/cm(3) (low dose), 1.4 x 10(6)
particles/cm(3) (middle dose), and 2.9 x 10(6) particles/cm(3) (high dose) for 6
h/day in an inhalation chamber for 90 days. The lung function was measured every
week after the daily exposure, and the animals sacrificed after the 90-day exposure
period. Cellular differential counts and inflammatory measurements, such as
albumin, lactate dehydrogenase (LDH), and total protein, were also monitored in the
acellular bronchoalveolar lavage (BAL) fluid of the rats exposed to the silver
nanoparticles for 90 days. Among the lung function test measurements, the tidal
volume and minute volume showed a statistically significant decrease during the 90
days of silver nanoparticle exposure. Although no statistically significant
differences were found in the cellular differential counts, the inflammation
measurements increased in the high-dose female rats. Meanwhile, histopathological
examinations indicated dose-dependent increases in lesions related to silver
nanoparticle exposure, such as infiltrate mixed cell and chronic alveolar
inflammation, including thickened alveolar walls and small granulomatous lesions.
Therefore, when taken together, the decreases in the tidal volume and minute volume
and other inflammatory responses after prolonged exposure to silver nanoparticles
would seem to indicate that nanosized particle inhalation exposure can induce lung
function changes, along with inflammation, at much lower mass dose concentrations
when compared to submicrometer particles.
AN - WOS:000255370500003
AU - Sung, J. H.
AU - Ji, J. H.
AU - Yoon, J. U.
AU - Kim, D. S.
AU - Song, M. Y.
AU - Jeong, J.
AU - Han, B. S.
AU - Han, J. H.
AU - Chung, Y. H.
AU - Kim, J.
AU - Kim, T. S.
AU - Chang, H. K.
AU - Lee, E. J.
AU - Lee, J. H.
AU - Yu, I. J.
DO - 10.1080/08958370701874671
IS - 6
PY - 2008
SN - 0895-8378
1091-7691
SP - 567-574
ST - Lung function changes in Sprague-Dawley rats after prolonged inhalation
exposure to silver nanoparticles
T2 - INHALATION TOXICOLOGY
TI - Lung function changes in Sprague-Dawley rats after prolonged inhalation
exposure to silver nanoparticles
VL - 20
ID - 6105
ER -
TY - JOUR
AB - Cell signaling for T-cell growth, differentiation, and apoptosis is initiated
in the cholesterol-rich microdomains of the plasma membrane known as lipid rafts.
Herein, we investigated whether enrichment of membrane cholesterol in lipid rafts
affects antigen-specific CD4 T-helper cell functions. Enrichment of membrane
cholesterol by 40-50% following squalene administration in mice was paralleled by
an increased number of resting CD4 T helper cells in periphery. We also observed
sensitization of the Th1 differentiation machinery through co-localization of IL-2R
alpha, IL-4R alpha, and IL-12R beta 2 subunits with GM1 positive lipid rafts, and
increased STAT-4 and STAT-5 phosphorylation following membrane cholesterol
enrichment. Antigen stimulation or CD3/CD28 polyclonal stimulation of membrane
cholesterol-enriched, resting CD4 T-cells followed a path of Th1 differentiation,
which was more vigorous in the presence of increased IL-12 secretion by APCs
enriched in membrane cholesterol. Enrichment of membrane cholesterol in antigen-
specific, autoimmune Th1 cells fostered their organ-specific reactivity, as
confirmed in an autoimmune mouse model for diabetes. However, membrane cholesterol
enrichment in CD4(+) Foxp3(+) T-reg cells did not alter their suppressogenic
function. These findings revealed a differential regulatory effect of membrane
cholesterol on the function of CD4 T-cell subsets. This first suggests that
membrane cholesterol could be a new therapeutic target to modulate the immune
functions, and second that increased membrane cholesterol in various
physiopathological conditions may bias the immune system toward an inflammatory Th1
type response.
AN - WOS:000305652700024
AU - Surls, J.
AU - Nazarov-Stoica, C.
AU - Kehl, M.
AU - Olsen, C.
AU - Casares, S.
AU - Brumeanu, T. D.
C7 - e38733
DA - JUN 19
DO - 10.1371/journal.pone.0038733
IS - 6
PY - 2012
SN - 1932-6203
ST - Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an
Inflammatory Response
T2 - PLOS ONE
TI - Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an
Inflammatory Response
VL - 7
ID - 6469
ER -
TY - JOUR
AB - Oral exposure to nanomaterials is a current concern, asking for innovative
biological test systems to assess their safety, especially also in conditions of
inflammatory disorders. Aim of this study was to develop a 3D intestinal model,
consisting of Caco-2 cells and two human immune cell lines, suitable to assess
nanomaterial toxicity, in either healthy or diseased conditions. Human macrophages
(THP-1) and human dendritic cells (MUTZ-3) were embedded in a collagen scaffold and
seeded on the apical side of transwell inserts. Caco-2 cells were seeded on top of
this layer, forming a 3D model of the intestinal mucosa. Toxicity of engineered
nanoparticles (NM101 TiO2, NM300 Ag, Au) was evaluated in non-inflamed and inflamed
co-cultures, and also compared to non-inflamed Caco-2 monocultures. Inflammation
was elicited by IL-1, and interactions with engineered NPs were addressed by
different endpoints. The 3D co-culture showed well preserved ultrastructure and
significant barrier properties. Ag NPs were found to be more toxic than TiO2 or Au
NPs. But once inflamed with IL-1, the co-cultures released higher amounts of IL-8
compared to Caco-2 monocultures. However, the cytotoxicity of Ag NPs was higher in
Caco-2 monocultures than in 3D co-cultures. The naturally higher IL-8 production in
the co-cultures was enhanced even further by the Ag NPs. This study shows that it
is possible to mimic inflamed conditions in a 3D co-culture model of the intestinal
mucosa. The fact that it is based on three easily available human cell lines makes
this model valuable to study the safety of nanomaterials in the context of
inflammation.
AN - WOS:000371810900006
AU - Susewind, J.
AU - Carvalho-Wodarz, C. D.
AU - Repnik, U.
AU - Collnot, E. M.
AU - Schneider-Daum, N.
AU - Griffiths, G. W.
AU - Lehr, C. M.
DA - JAN 2
DO - 10.3109/17435390.2015.1008065
IS - 1
PY - 2016
SN - 1743-5390
1743-5404
SP - 53-62
ST - A 3D co-culture of three human cell lines to model the inflamed intestinal
mucosa for safety testing of nanomaterials
T2 - NANOTOXICOLOGY
TI - A 3D co-culture of three human cell lines to model the inflamed intestinal
VL - 10
ID - 6222
ER -
TY - JOUR
AB - Oral exposure to nanomaterials is a current concern, asking for innovative
biological test systems to assess their safety, especially also in conditions of
inflammatory disorders. Aim of this study was to develop a 3D intestinal model,
consisting of Caco-2 cells and two human immune cell lines, suitable to assess
nanomaterial toxicity, in either healthy or diseased conditions. Human macrophages
(THP-1) and human dendritic cells (MUTZ-3) were embedded in a collagen scaffold and
seeded on the apical side of transwell inserts. Caco-2 cells were seeded on top of
this layer, forming a 3D model of the intestinal mucosa. Toxicity of engineered
nanoparticles (NM101 TiO2, NM300 Ag, Au) was evaluated in non-inflamed and inflamed
co-cultures, and also compared to non-inflamed Caco-2 monocultures. Inflammation
was elicited by IL-1β, and interactions with engineered NPs were addressed by
different endpoints. The 3D co-culture showed well preserved ultrastructure and
significant barrier properties. Ag NPs were found to be more toxic than TiO2 or Au
NPs. But once inflamed with IL-1β, the co-cultures released higher amounts of IL-8
compared to Caco-2 monocultures. However, the cytotoxicity of Ag NPs was higher in
Caco-2 monocultures than in 3D co-cultures. The naturally higher IL-8 production in
the co-cultures was enhanced even further by the Ag NPs. This study shows that it
is possible to mimic inflamed conditions in a 3D co-culture model of the intestinal
mucosa. The fact that it is based on three easily available human cell lines makes
this model valuable to study the safety of nanomaterials in the context of
inflammation. © 2015 Helmholtz-Zentrum für Infektionsforschung.
AU - Susewind, J.
AU - De Souza Carvalho-Wodarz, C.
AU - Repnik, U.
AU - Collnot, E. M.
AU - Schneider-Daum, N.
AU - Griffiths, G. W.
AU - Lehr, C. M.
DB - Scopus
DO - 10.3109/17435390.2015.1008065
IS - 1
KW - Caco-2
cytotoxicity
intestine
nanoparticles
Caco-2 Cells
Humans
Inflammation
Interleukin-8
Intestinal Mucosa
Metal Nanoparticles
Nanostructures
Titanium
collagen
gold nanoparticle
interleukin 1beta
interleukin 8
messenger RNA
silver nanoparticle
metal nanoparticle
nanomaterial
titanium
titanium dioxide
Article
CACO 2 cell line
cell ultrastructure
cell viability
coculture
controlled study
cytokine production
cytokine release
dendritic cell
drug cytotoxicity
enteritis
human
human cell
in vitro study
intestine mucosa
macrophage culture
monoculture
particle size
priority journal
protein expression
three dimensional imaging
tight junction
transepithelial resistance
biosynthesis
Caco-2 cell line
chemically induced
drug effects
inflammation
M3 - Article
PY - 2016
SP - 53-62
ST - A 3D co-culture of three human cell lines to model the inflamed intestinal
T2 - Nanotoxicology
TI - A 3D co-culture of three human cell lines to model the inflamed intestinal
84959544878&doi=10.3109%2f17435390.2015.1008065&partnerID=40&md5=e99c351f18824c404b
58035fe3d658eb
VL - 10
ID - 5622
ER -
TY - JOUR
AB - A nanotopographic noble metal (Ag, Au, Pd) coating has been applied on
commercial urinary catheters and used in more than 80,000 patients, with good
clinical results. We have previously evaluated the biocompatibility of different
variations of this coating, showing high cellular viability and function in vitro.
However, the reasons for good clinical and preclinical behavior are not known. This
in vivo study aimed to investigate the soft tissue periimplant reaction to five
coatings with systematically altered noble metal ratios after 1, 3, and 21 days of
implantation in rats. The results show that coatings of silver only, or silver with
medium amounts of gold and low-medium palladium content were superior to other
tested coatings. Such surfaces were during the first days after implantation
associated with a decreased recruitment of inflammatory cells to implant close
exudates, a lower percentage of neutrophils, higher cell viability, and lower
production of monocyte chemoattractant protein-1 (MCP-1), compared to the other
coatings and uncoated silicone (PDMS) control. In contrast, the addition of higher
concentrations of gold and palladium to silver induced a thicker soft tissue
capsule. Coatings with high concentration of palladium induced the thickest fibrous
capsule after 21 days of implantation. The study demonstrates that by varying the
noble metal ratio at implant surfaces it is possible to modulate inflammation and
fibrosis in soft tissue. © 2009 Wiley Periodicals, Inc.
AU - Suska, F.
AU - Svensson, S.
AU - Johansson, A.
AU - Emanuelsson, L.
AU - Karlholm, H.
AU - Ohrlander, M.
AU - Thomsen, P.
DB - Scopus
DO - 10.1002/jbm.b.31492
IS - 1
KW - Antibacterial coatings
Fibrous capsule
Inflammation
Noble metals
Viability
Animals
Cell Survival
Chemokine CCL2
Female
Metals
Rats
Transforming Growth Factor beta1
Biocompatibility
Control surfaces
Palladium
Pathology
Precious metals
Protective coatings
Silicone coatings
Silicones
Silver
gold
metal
palladium
silicone
silver
Cell viability
Cellular viability
Chemoattractants
High concentration
Implant surface
In-vitro
In-vivo
Inflammatory cells
Noble metal coatings
Soft tissue
Urinary catheters
animal cell
animal experiment
article
biocompatibility
cell viability
control group
controlled study
female
implantation
in vitro study
in vivo study
inflammatory cell
material coating
neutrophil
nonhuman
rat
Gold coatings
M3 - Article
PY - 2010
SP - 86-94
ST - In vivo evaluation of noble metal coatings
TI - In vivo evaluation of noble metal coatings
74749097188&doi=10.1002%2fjbm.b.31492&partnerID=40&md5=7a9750daadf48eadf07e366dd94b
28e8
VL - 92
ID - 5711
ER -
TY - JOUR
AB - Nitinol is a nickel-titanium alloy widely used in medical devices for its
unique pseudoelastic and shape-memory properties. However, nitinol can release
potentially hazardous amounts of nickel, depending on surface manufacturing
yielding different oxide thicknesses and compositions. Furthermore, nitinol medical
devices can be implanted throughout the body and exposed to extremes in pH and
reactive oxygen species (ROS), but few tools exist for evaluating nickel release
under such physiological conditions. Even in cardiovascular applications, where
nitinol medical devices are relatively common and the blood environment is well
understood, there is a lack of information on how local inflammatory conditions
after implantation might affect nickel ion release. For this study, nickel release
from nitinol wires of different finishes was measured in pH conditions and at ROS
concentrations selected to encompass and exceed literature reports of extracellular
pH and ROS. Results showed increased nickel release at levels of pH and ROS
reported to be physiological, with decreasing pH and increasing concentrations of
hydrogen peroxide and NaOCl/HOCl having the greatest effects. The results support
the importance of considering the implantation site when designing studies to
predict nickel release from nitinol and underscore the value of understanding the
chemical milieu at the device-tissue interface.
AN - WOS:000788406700001
AU - Sussman, E. M.
AU - Shi, H. Y.
AU - Turner, P. A.
AU - Saylor, D. M.
AU - Weaver, J. D.
AU - Simon, D. D.
AU - Takmakov, P.
AU - Sivan, S.
AU - Shin, H. Y.
AU - Di Prima, M. A.
AU - Godar, D. E.
C6 - APR 2022
DA - JUN
DO - 10.1007/s40830-022-00364-3
IS - 2
PY - 2022
SN - 2199-384X
2199-3858
SP - 98-106
ST - Nitinol Release of Nickel under Physiological Conditions: Effects of Surface
Oxide, pH, Hydrogen Peroxide, and Sodium Hypochlorite
T2 - SHAPE MEMORY AND SUPERELASTICITY
TI - Nitinol Release of Nickel under Physiological Conditions: Effects of Surface
Oxide, pH, Hydrogen Peroxide, and Sodium Hypochlorite
VL - 8
ID - 6767
ER -
TY - JOUR
AB - The three heavy metals, mercury, gold and silver commonly and specifically
induce aberrant immunological responses leading to autoimmune disorders in
genetically susceptible animals and humans. The disorders are characterized by
autoantibody production, increases in serum IgG and IgE, polyclonal activation of B
and T lymphocytes and renal immune complex deposition and glomerulonephritis. Mast
cells play key roles in allergic and inflammatory reactions. A growing body of
evidence suggests that mast cells are key players in innate and adaptive immunity
and involved in autoimmune diseases. Mast cells are also direct targets for
autoimmunity-inducing metals both in vitro and in vivo and play a role in the
development of metal-induced autoimmune disorders. The three metals specifically
modulate mast cell function, including degranulation and secretion of arachidonic
acid metabolites and cytokines such as interleukin-4. Divergent signaling
components, including mitogen-activated protein kinase activation, reactive oxygen
and nitric oxide generation and Ca2+ influx are modulated by the metals.
Furthermore, the metals have considerable impacts on mast cell survival, which also
species seems to be involved in the development of metal-induced autoimmune
disorders. In this review, we provide an overview of recent advances in our
understanding of the impacts of the three metals on mast cell signaling, function
and survival and their possible roles in the pathologies of metal-induced
autoimmunity. © 2011 Bentham Science Publishers.
AU - Suzuki, Y.
AU - Inoue, T.
AU - Ra, C.
DB - Scopus
DO - 10.2174/138161211798357917
IS - 34
KW - Apoptosis
Mitochondria
Necrosis
Nitric oxide
Ros
2 (2 amino 3 methoxyphenyl)chromone
calcium channel L type
DNA (apurinic or apyrimidinic site) lyase
gold
histamine
hydrogen peroxide
interleukin 13
interleukin 4
interleukin 6
mercuric chloride
mercury
nitric oxide
protein kinase Fyn
protein kinase Lyn
serotonin
silver
silver nanoparticle
silver nitrate
STAT6 protein
superoxide
tyrosine
vasculotropin
adaptive immunity
autoimmunity
cell death
cell function
cell survival
cytokine production
cytotoxicity
DNA binding
enzyme activation
histamine release
human
humoral immunity
immunomodulation
mast cell
nonhuman
priority journal
review
serotonin release
signal transduction
M3 - Review
PY - 2011
SP - 3805-3814
ST - Autoimmunity-inducing metals (Hg, Au and Ag) modulate mast cell signaling,
function and survival
TI - Autoimmunity-inducing metals (Hg, Au and Ag) modulate mast cell signaling,
function and survival
83455238445&doi=10.2174%2f138161211798357917&partnerID=40&md5=fdeecb98ec5ff8839d368
a4e7640dce4
VL - 17
ID - 5705
ER -
TY - JOUR
AB - Inhaled nanoparticles have a high deposition rate in the alveolar units of
the deep lung. The alveolar epithelium is composed of type-I and type-II epithelial
cells (ATI and ATII respectively) and is bathed in pulmonary surfactant. The effect
of native human ATII cell secretions on nanoparticle toxicity is not known. We
investigated the cellular uptake and toxicity of silver nanowires (AgNWs; 70 nm
diameter, 1.5 mu m length) with human ATI-like cells (TT1), in the absence or
presence of Curosurf (R) (a natural porcine pulmonary surfactant with a low amount
of protein) or harvested primary human ATII cell secretions (HAS; containing both
the complete lipid as well as the full protein complement of human pulmonary
surfactant i.e. SP-A, SP-B, SP-C and SP-D). We hypothesised that Curosurf (R) or
HAS would confer improved protection for TT1 cells, limiting the toxicity of AgNWs.
In agreement with our hypothesis, HAS reduced the inflammatory and reactive oxygen
species (ROS)-generating potential of AgNWs with exposed TT1 cells. For example,
IL-8 release and ROS generation was reduced by 38% and 29%, respectively, resulting
in similar levels to that of the non-treated controls. However in contrast to our
hypothesis, Curosurf (R) had no effect. We found a significant reduction in AgNW
uptake by TT1 cells in the presence of HAS but not Curosurf. Furthermore, we show
that the SP-A and SP-D are likely to be involved in this process as they were found
to be specifically bound to the AgNWs. While ATI cells appear to be protected by
HAS, evidence suggested that ATII cells, despite no uptake, were vulnerable to AgNW
exposure (indicated by increased IL-8 release and ROS generation and decreased
intracellular SP-A levels one day post-exposure). This study provides unique
findings that may be important for the study of lung epithelial-endothelial
translocation of nanoparticles in general and associated toxicity within the
alveolar unit.
AN - WOS:000355987300013
AU - Sweeney, S.
AU - Zambianchi, M.
AU - Chen, S.
AU - Gow, A.
AU - Schwander, S.
AU - Zhang, J. F.
AU - Chung, K. F.
AU - Ryan, M. P.
AU - Porter, A. E.
AU - Tetley, T. D.
DO - 10.1039/c5nr01496d
IS - 23
PY - 2015
SN - 2040-3364
2040-3372
SP - 10398-10409
ST - Silver nanowire interactions with primary human alveolar type-II epithelial
cell secretions: contrasting bioreactivity with human alveolar type-I and type-II
epithelial cells
T2 - NANOSCALE
TI - Silver nanowire interactions with primary human alveolar type-II epithelial
cell secretions: contrasting bioreactivity with human alveolar type-I and type-II
epithelial cells
VL - 7
ID - 6440
ER -
TY - JOUR
AB - Inhaled nanoparticles have a high deposition rate in the alveolar units of
the deep lung. The alveolar epithelium is composed of type-I and type-II epithelial
cells (ATI and ATII respectively) and is bathed in pulmonary surfactant. The effect
of native human ATII cell secretions on nanoparticle toxicity is not known. We
investigated the cellular uptake and toxicity of silver nanowires (AgNWs; 70 nm
diameter, 1.5 μm length) with human ATI-like cells (TT1), in the absence or
presence of Curosurf® (a natural porcine pulmonary surfactant with a low amount of
protein) or harvested primary human ATII cell secretions (HAS; containing both the
complete lipid as well as the full protein complement of human pulmonary surfactant
i.e. SP-A, SP-B, SP-C and SP-D). We hypothesised that Curosurf® or HAS would confer
improved protection for TT1 cells, limiting the toxicity of AgNWs. In agreement
with our hypothesis, HAS reduced the inflammatory and reactive oxygen species
(ROS)-generating potential of AgNWs with exposed TT1 cells. For example, IL-8
release and ROS generation was reduced by 38% and 29%, respectively, resulting in
similar levels to that of the non-treated controls. However in contrast to our
hypothesis, Curosurf® had no effect. We found a significant reduction in AgNW
uptake by TT1 cells in the presence of HAS but not Curosurf. Furthermore, we show
that the SP-A and SP-D are likely to be involved in this process as they were found
to be specifically bound to the AgNWs. While ATI cells appear to be protected by
HAS, evidence suggested that ATII cells, despite no uptake, were vulnerable to AgNW
exposure (indicated by increased IL-8 release and ROS generation and decreased
intracellular SP-A levels one day post-exposure). This study provides unique
findings that may be important for the study of lung epithelial-endothelial
translocation of nanoparticles in general and associated toxicity within the
alveolar unit. © 2014 The Royal Society of Chemistry.
AU - Sweeney, S.
AU - Zambianchi, M.
AU - Chen, S.
AU - Gow, A.
AU - Schwander, S.
AU - Zhang, J.
AU - Chung, K. F.
AU - Ryan, M. P.
AU - Porter, A. E.
AU - Tetley, T. D.
DB - Scopus
DO - 10.1039/c5nr01496d
IS - 23
KW - Cell Line
Cell Survival
Cytokines
Epithelial Cells
Humans
Materials Testing
Metal Nanoparticles
Nanowires
Pulmonary Alveoli
Pulmonary Surfactants
Silver
Biological organs
Cytology
Deposition rates
Electric resistance measurement
Nanoparticles
Phospholipids
Proteins
Silver nanowires
Toxicity
cytokine
lung surfactant
metal nanoparticle
nanowire
silver
Alveolar epitheliums
Cellular uptake
Epithelial cells
High deposition rates
Nanoparticle toxicity
Protein complement
Pulmonary surfactants
Ros generations
cell line
cell survival
chemistry
drug effects
epithelium cell
human
immunology
lung alveolus
materials testing
pathology
Cells
M3 - Article
PY - 2015
SP - 10398-10409
ST - Silver nanowire interactions with primary human alveolar type-II epithelial
cell secretions: Contrasting bioreactivity with human alveolar type-I and type-II
epithelial cells
T2 - Nanoscale
TI - Silver nanowire interactions with primary human alveolar type-II epithelial
cell secretions: Contrasting bioreactivity with human alveolar type-I and type-II
epithelial cells
84930842493&doi=10.1039%2fc5nr01496d&partnerID=40&md5=91942f6d40274812671d85efc6bb5
cda
VL - 7
ID - 5636
ER -
TY - JOUR
AB - Nanomaterials, substances below 100 nm, are increasingly used in medical
diagnosis and treatment every day. The use of such materials has helped deliver
drugs across the blood-brain barrier, alleviate allergy symptoms, specifically
target cancer or HIV cells, and more. However, the tunable characteristics of such
materials have not been perfected. The different materials, sizes, shapes, and
structures have different responses on the body. This paper will investigate the
successful treatments made with nanoparticles and some general health effects. A
review of the literature revealed an inflammatory response and an increased
production of reactive oxidative species (ROS) to be common immune responses to
nanomaterial use. The mechanisms by which the inflammatory response and ROS
production occur will also be discussed. © 2012 Springer Science+Business Media,
LLC.
AU - Syed, S.
AU - Zubair, A.
AU - Frieri, M.
DB - Scopus
DO - 10.1007/s11882-012-0302-3
IS - 1
KW - Advances
Allergy
Immune response
Immunology
Nanomaterials
NF-κB pathway
Nuclear factor-κB
acetylcysteine
aluminum oxide
carbon nanotube
chitosan
gamma interferon
gold nanoparticle
I kappa B alpha
interleukin 12
interleukin 6
nanomaterial
quantum dot
saquinavir
silicon dioxide
silver nanoparticle
titanium dioxide
antiviral activity
cytokine release
cytokine response
cytotoxicity
genotoxicity
human
immune response
inflammation
nanoanalysis
nanopharmaceutics
nanotoxicology
nonhuman
oxidative stress
particle size
review
silicosis
Th1 Th2 balance
toxicity testing
M3 - Review
PY - 2013
SP - 50-57
ST - Immune response to nanomaterials: Implications for medicine and literature
review
T2 - Current Allergy and Asthma Reports
TI - Immune response to nanomaterials: Implications for medicine and literature
review
84872609432&doi=10.1007%2fs11882-012-0302-
3&partnerID=40&md5=c2e3c04fd5f1575990bfdc0711e0dfbc
VL - 13
ID - 5733
ER -
TY - JOUR
AB - In recent years, there has been a significant increase in the clinical use of
organometallic compounds and metal complexes for therapeutic purposes including
treatment of inflammatory bowel diseases (IBD). Their action is based on the
inhibition of the inflow of pro-inflammatory cytokines, the elimination of free
radicals or the modulation of intestinal microbiota. In addition, these compounds
are intended for use in the diagnosis and treatment of colorectal cancer (CRC)
which is often a consequence of IBD. The aim of this study is to critically discuss
recent findings on the use of organometallic compounds and metal complexes in the
treatment of IBD and CRC and suggest future trends in drug design. © 2019 by the
AU - Szczepaniak, A.
AU - Fichna, J.
C7 - 398
DB - Scopus
DO - 10.3390/biom9090398
IS - 9
KW - Colorectal cancer
Free radicals
Inflammatory bowel diseases
Metal complexes
Organometallic compounds
Animals
Carbon Monoxide
Colorectal Neoplasms
Humans
aminosalicylic acid
carbon monoxide
epidermal growth factor receptor
free radical
gelatinase B
gold complex
gold nanoparticle
interleukin 10
interleukin 12
interleukin 1beta
interleukin 6
interleukin 8
iridium
metal complex
NEDD8 protein
organometallic compound
rhodium
ruthenium
salazosulfapyridine
silver nanoparticle
STAT3 protein
triphenyltin
unindexed drug
zinc
Article
cancer cell line
cancer chemotherapy
cancer radiotherapy
chemical structure
colorectal cancer
cytotoxicity
DNA damage
drug synthesis
endoscopy
enzyme activity
human
IC50
intestine flora
microbial community
nonhuman
oxidative stress
animal
colorectal tumor
metabolism
M3 - Article
PY - 2019
ST - Organometallic compounds and metal complexes in current and future treatments
of inflammatory bowel disease and colorectal cancer-a critical review
T2 - Biomolecules
TI - Organometallic compounds and metal complexes in current and future treatments
of inflammatory bowel disease and colorectal cancer-a critical review
85071439981&doi=10.3390%2fbiom9090398&partnerID=40&md5=51fd985c652595c3c1a8a15c6faf
a8d4
VL - 9
ID - 5396
ER -
TY - JOUR
AB - Generation of well-defined potential metallotherapeutics for cancer
treatment, one of the most population-threatening diseases, is challenging and an
active area of modern research in view of their unique properties and thus multiple
possible pathways of action in cells. Specifically, Schiff base ligands were
recognized as very promising building blocks for the construction of stable and
active complexes of numerous geometries and topologies. Incorporation of Ag(I) ions
allows for the formation of flat complexes with potential unoccupied coordination
sites, thus giving rise to specific interactions between the metallotherapeutic and
biomolecule of interest. Herein, we present the design, synthesis and
characterization of new Schiff base ligand L and its Ag(I) bimetallic complex
[Ag2L2]2+ with two planar moieties formed around the metal ions and connected
through cyclohexane rings, confirmed by X-ray measurements. The compounds were
described in context of their potential use as anticancer drugs through DNA and BSA
binding pathways by several spectroscopic methods (CD, UV-Vis, fluorescence). We
revealed that both, L and [Ag2L2]2+, interact with similar affinity with CT-DNA
(Kb~106 M−1), while they differ in the type and strength of interactions with the
model albumin–BSA. [Ag2L2]2+ binds BSA in both a dynamic and static manner with the
Ksv = 8.8 × 104 M−1 in the Trp-134 and Trp-213 sites, whereas L interacts with BSA
only dynamically (KSV = 2.4 × 104 M−1). This found further confirmation in the CD
studies which revealed a reduction in α-helix content in the albumin of 16% in
presence of [Ag2L2]2+. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Szymańska, M.
AU - Pospieszna-Markiewicz, I.
AU - Mańka, M.
AU - Insińska-Rak, M.
AU - Dutkiewicz, G.
AU - Patroniak, V.
AU - Fik-Jaskółka, M. A.
C7 - 1449
DB - Scopus
DO - 10.3390/biom11101449
IS - 10
Anticancer
BSA
CT-DNA
Schiff base
Silver(I) complex
DNA
DNA-Binding Proteins
Humans
Ligands
Neoplasms
Protein Binding
Schiff Bases
Serum Albumin, Bovine
Silver
acetic acid
albumin
cisplatin
cyclohexane
doxorubicin
scoparone
silver
thiazole derivative
DNA binding protein
ligand
protein binding
alpha helix
apoptosis
Article
base pairing
binding affinity
cancer therapy
carbon nuclear magnetic resonance
chemical structure
circular dichroism
computer model
crystal structure
cytotoxicity
drug solubility
drug synthesis
fluorescence
herbal medicine
hydrogen bond
hydrophobicity
inhibition constant
molecular docking
molecular recognition
spectrofluorometry
spectroscopy
static electricity
stoichiometry
thermostability
X ray
X ray diffraction
chemistry
drug effect
human
neoplasm
synthesis
M3 - Article
PY - 2021
ST - Synthesis and spectroscopic investigations of schiff base ligand and its
bimetallic ag(I) complex as dna and bsa binders
T2 - Biomolecules
TI - Synthesis and spectroscopic investigations of schiff base ligand and its
bimetallic ag(I) complex as dna and bsa binders
85116147194&doi=10.3390%2fbiom11101449&partnerID=40&md5=965b1bbd9f399bee714738414ad
adc5f
VL - 11
ID - 5165
ER -
TY - JOUR
AB - Due to the widespread use of indium tin oxide (ITO), it is important to
investigate its effect on human health. In this study, we evaluated the cellular
effects of ITO nanoparticles (NPs), indium chloride (InCl3) and tin chloride
(SnCl3) using human lung epithelial A549 cells. Transmission electron microscopy
and inductively coupled plasma mass spectrometry were employed to study cellular
ITO NP uptake. Interestingly, greater uptake of ITO NPs was observed, as compared
with soluble salts. ITO NP species released could be divided into two types:
'indium release ITO' or 'tin release ITO'. We incubated A549 cells with indium
release ITO, tin release ITO, InCl3 or SnCl2 and investigated oxidative stress,
proinflammatory response, cytotoxicity and DNA damage. We found that intracellular
reactive oxygen species were increased in cells incubated with indium release ITO,
but not tin release ITO, InCl3 or SnCl2. Messenger RNA and protein levels of the
inflammatory marker, interleukin-8, also increased following exposure to indium
release ITO. Furthermore, the alkaline comet assay revealed that intracellular
accumulation of indium ions induced DNA damage. Our results demonstrate that the
accumulation of ionic indium, but not ionic tin, from ITO NPs in the intracellular
matrix has extensive cellular effects.
AN - WOS:000370299900010
AU - Tabei, Y.
AU - Sonoda, A.
AU - Nakajima, Y.
AU - Biju, V.
AU - Makita, Y.
AU - Yoshida, Y.
AU - Horie, M.
DA - FEB
DO - 10.1093/jb/mvv098
IS - 2
PY - 2016
SN - 0021-924X
1756-2651
SP - 225-237
ST - Intracellular accumulation of indium ions released from nanoparticles induces
oxidative stress, proinflammatory response and DNA damage
T2 - JOURNAL OF BIOCHEMISTRY
TI - Intracellular accumulation of indium ions released from nanoparticles induces
oxidative stress, proinflammatory response and DNA damage
VL - 159
ID - 6710
ER -
TY - JOUR
AB - Titanium dioxide nanoparticles (TiO2 NPs) are widely used in industry as a
white pigment (paints, paper industry and toothpastes), photocatalysts
(environmental decontamination and photovoltaic cells), inorganic UV filter
(sunscreens and personal care products) and as a food additive (E171) and
antimicrobial food packaging material. Silver nanoparticles (Ag NPs) are used in
photonics, microelectronics, catalysis and medicine due to their catalytic
activity, magnetic and optical polarizability, electrical and thermal
conductivities and enhanced Raman scattering. They also have antibacterial,
antifungal and antiviral activities, as well as anti-inflammatory potential. The
huge increase in the use of nano-based products, mainly metallic NPs, implies the
presence of nano-materials in the environment, and hence, the unintentional human
ingestion through water or foods (gastrointestinal tract is the main pathway of NPs
intake in humans). The presence of TiO2 NPs and Ag NPs in seafood samples was
firstly established using an ultrasound assisted enzymatic hydrolysis procedure and
sp-ICP-MS analysis. Several clams, cockles, mussels, razor clams, oysters and
variegated scallops, which contain TiO2 NPs and Ag NPs, were subjected to an in
vitro digestion process simulating human gastrointestinal digestion in the stomach
and in the small and large intestine to determine the bioaccessibility of these
NPs. Caco-2 cells were selected as model of human intestinal epithelium for
transport studies because of the development of membrane transporters that are
responsible for the uptake of chemicals. Parameters as transepithelial electrical
resistance (TEER) and permeability of Lucifer Yellow were studied for establishing
cell monolayer integrity. TiO2 NPs and Ag NPs transport as well as total Ti and Ag
concentrations passing through the gastrointestinal epithelial barrier model (0-2
h) were assessed by sp-ICP-MS and ICP-MS in several molluscs.
AN - WOS:000668000500002
AU - Taboada-Lopez, M. V.
AU - Leal-Martinez, B. H.
AU - Dominguez-Gonzalez, R.
AU - Bermejo-Barrera, P.
AU - Taboada-Antelo, P.
AU - Moreda-Pineiro, A.
C6 - MAY 2021
C7 - 122494
DA - OCT 1
DO - 10.1016/j.talanta.2021.122494
PY - 2021
SN - 0039-9140
1873-3573
ST - Caco-2 in vitro model of human gastrointestinal tract for studying the
absorption of titanium dioxide and silver nanoparticles from seafood
T2 - TALANTA
TI - Caco-2 in vitro model of human gastrointestinal tract for studying the
absorption of titanium dioxide and silver nanoparticles from seafood
VL - 233
ID - 6060
ER -
TY - JOUR
AB - Background: Nitric oxide (NO) production by the action of the inducible
nitric oxide synthase (iNOS or NOS2) is increased in tissues that are stimulated by
cytokine and endotoxins. The role of NO in the pathogenesis of chronic viral
hepatitis is not fully understood but it seems that its overproduction is
responsible for the pathological changes under inflammatory conditions. Aim: In
this paper, we analyzed the correlation between immunohistochemical expression of
iNOS and liver fibrosis in chronic viral hepatitis. Materials and Methods: Liver
biopsies from patients diagnosed with chronic viral hepatitis B and C were embedded
in paraffin and further used for histological staining and immunohistochemical
reactions to detect the expression of iNOS and TGF-beta 1. The degree of liver
fibrosis was established using special staining (trichromic Masson and Gomori's
silver impregnation). Results: In samples with low degree of fibrosis, we observed
a discrete positivity for iNOS in periportal hepatocytes and the
immunohistochemical reaction for TGF-beta 1 were limited to the endothelial cells
of liver sinusoids and pro-inflammatory cells from the portal tracts. Positive
reaction for TGF-beta 1 increased with the degree of liver fibrosis, while the
expression of iNOS was enhanced in hepatocytes, as well as in bile ducts and
endothelial cells. Conclusions: Infection with hepatitis B and C viruses induces
iNOS expression in hepatocytes, suggesting that NO overproduction might have an
important role in progression of chronic viral hepatitis to cirrhosis.
AN - WOS:000342868500005
AU - Tache, D. E.
AU - Stanciulescu, C. E.
AU - Banita, I. M.
AU - Purcaru, S. O.
AU - Andrei, A. M.
AU - Comanescu, V.
AU - Pisoschi, C. G.
IS - 2
PY - 2014
SN - 1220-0522
SP - 539-543
ST - Inducible nitric oxide synthase expression (iNOS) in chronic viral hepatitis
and its correlation with liver fibrosis
T2 - ROMANIAN JOURNAL OF MORPHOLOGY AND EMBRYOLOGY
TI - Inducible nitric oxide synthase expression (iNOS) in chronic viral hepatitis
and its correlation with liver fibrosis
VL - 55
ID - 6746
ER -
TY - JOUR
AB - Raptinal is a novel antineoplastic agent that induces an expeditious
intrinsic apoptotic pathway, in addition to the shutdown of mitochondrial function
for cancerous cells, because of silver nanoparticles (AgNPs) that have been shown
to provide a worthy approach to overcome tumors. In this study, Both Raptinal and
Raptinal-loaded silver nanoparticles (AgNPs) were tested as the first time in
hepatocellular carcinoma–induced mice to evaluate its efficacy and targeting to
HCC. Seventy-two albino male mice of comparable age were classified into six
groups; early stage of HCC was induced using diethyl nitrosamine (DEN)/carbon
tetrachloride (CCL4). Liver function was assessed in all groups using ALT, AST,
total bilirubin, and alpha-fetoprotein (AFP) as well as histopathological
examination. Quantitative gene expression of key apoptotic gene markers p53,
cytochrome c, and caspase 3 was assessed in all liver homogenates. The results
showed that Raptinal-loaded AgNPs group had significant increase in both apoptotic
genes of cytochrome c and Caspase 3 at P = 0.0001 compared with Raptinal-free drug
group. AFP levels were significantly decreased in Raptinal-loaded AgNPs group
compared with both Raptinal-free drug and HCC groups at P = 0.0001. Degenerative
changes in the hepatocytes with focal necrosis and inflammatory cell infiltration
in histopathology confirm the biochemical analysis. Our study is considered one of
the first studies using Raptinal in vivo. Moreover, it showed that Raptinal and/or
the combination between Raptinal and AgNPs showed a promising therapeutic agent in
treating early HCC. [Figure not available: see fulltext.] © 2020, Springer-Verlag
GmbH Germany, part of Springer Nature.
AU - Taha, H.
AU - Elfar, N.
AU - Haffez, H.
AU - Hassan, Z. A.
DB - Scopus
DO - 10.1007/s00210-020-01973-4
IS - 2
KW - Alpha-fetoprotein
Apoptosis
Hepatocellular carcinoma
Raptinal
alpha-Fetoproteins
Animals
Carcinoma, Hepatocellular
Caspase 3
Cyclopentanes
Cytochromes c
Fluorenes
Hepatocytes
Liver
Liver Neoplasms
Male
Metal Nanoparticles
Mice
Silver
alpha fetoprotein
bilirubin
carbon tetrachloride
caspase 3
cytochrome c
diethylnitrosamine
protein p53
raptinal
silver nanoparticle
unclassified drug
Casp3 protein, mouse
cyclopentane derivative
fluorene derivative
metal nanoparticle
silver
adult
alpha fetoprotein blood level
animal cell
animal experiment
animal model
animal tissue
Article
body weight
cell infiltration
controlled study
gene expression
gene expression level
histopathology
inflammatory cell
liver cell
liver cell carcinoma
liver function
liver function test
liver homogenate
male
marker gene
mouse
nonhuman
animal
blood
disease model
drug effect
genetics
liver
liver tumor
metabolism
pathology
M3 - Article
PY - 2021
SP - 279-289
ST - Raptinal silver nanoparticles: new therapeutic advances in hepatocellular
carcinoma mouse model
T2 - Naunyn-Schmiedeberg's Archives of Pharmacology
TI - Raptinal silver nanoparticles: new therapeutic advances in hepatocellular
carcinoma mouse model
85091168948&doi=10.1007%2fs00210-020-01973-
4&partnerID=40&md5=7a7656489b3277c7706ea54e46da1d8e
VL - 394
ID - 5173
ER -
TY - JOUR
AB - Medical device associated infections are a persistent medical problem which
has not found a comprehensive solution yet. Over the last decades, there have been
intense research efforts toward developing antibacterial coatings that could
potentially improve medical outcomes. Silver nanoparticles have attracted a great
deal of attention as a potent alternative to conventional antibiotics. Herein, we
present a biologically inspired approach to synthesize phospholipid encapsulated
silver nanoparticles and their surface immobilization to a functional plasma
polymer interlayer to generate antibacterial coatings. The antibacterial efficacy
of the coatings was evaluated against three medically relevant pathogens including
the Gram-positive Staphylococcus aureus and Staphylococcus epidermidis, and the
Gram-negative Pseudomonas aeruginosa. The innate immune response to the coatings
was assessed in vitro using primary bone marrow derived macrophages (BMDM). Any
potential cytotoxicity was studied with primary human dermal fibroblasts (HDFs).
Overall, the coatings had excellent inhibition of bacterial growth. We also
observed reduced expression of pro-inflammatory cytokines from BMDM which suggests
a reduced inflammatory response. The combined properties of coatings developed in
this study may make them a good candidate for application on medical devices such
as catheters and wound dressings. © 2015 American Chemical Society.
AU - Taheri, S.
AU - Cavallaro, A.
AU - Christo, S. N.
AU - Majewski, P.
AU - Barton, M.
AU - Hayball, J. D.
AU - Vasilev, K.
DB - Scopus
IS - 12
KW - antibacterial coating
phospholipid bilayer
phospholipid
phosphorus
polymer
silver
silver nanoparticle
Article
bacterial growth
biofilm
cell growth
cell viability
comparative study
conjugation
controlled study
human
human cell
immobilization
in vitro study
innate immunity
lipid bilayer
nanoencapsulation
precipitation
priority journal
surface property
synthesis
ultrasound
M3 - Article
PY - 2015
SP - 1278-1286
ST - Antibacterial Plasma Polymer Films Conjugated with Phospholipid Encapsulated
TI - Antibacterial Plasma Polymer Films Conjugated with Phospholipid Encapsulated
84962635896&doi=10.1021%2facsbiomaterials.5b00338&partnerID=40&md5=b0ae4b9e68c218a3
a2a9ce7eeedc7d1d
VL - 1
ID - 5594
ER -
TY - JOUR
AB - Infections arising from bacterial adhesion and colonization on medical device
surfaces are a significant healthcare problem. Silver based antibacterial coatings
have attracted a great deal of attention as a potential solution. This paper
reports on the development of a silver nanoparticles based antibacterial surface
that can be applied to any type of material surface. The silver nanoparticles were
surface engineered with a monolayer of 2-mercaptosuccinic acid, which facilitates
the immobilization of the nanoparticles to the solid surface, and also reduces the
rate of oxidation of the nanoparticles, extending the lifetime of the coatings. The
coatings had excellent antibacterial efficacy against three clinically significant
pathogenic bacteria i.e. Staphylococcus epidermidis, Staphylococcus aureus and
Pseudomonas aeruginosa. Studies with primary human fibroblast cells showed that the
coatings had no cytotoxicity invitro. Innate immune studies in cultures of primary
macrophages demonstrated that the coatings do not significantly alter the level of
expression of pro-inflammatory cytokines or the adhesion and viability of these
cells. Collectively, these coatings have an optimal combination of properties that
make them attractive for deposition on medical device surfaces such as wound
dressings, catheters and implants. © 2014 Elsevier Ltd.
AU - Taheri, S.
AU - Cavallaro, A.
AU - Christo, S. N.
AU - Smith, L. E.
AU - Majewski, P.
AU - Barton, M.
AU - Hayball, J. D.
AU - Vasilev, K.
DB - Scopus
IS - 16
KW - Antibacterial surfaces
Cytotoxicity
Infections
Innate inflammatory response
Medical devices
Animals
Cells, Cultured
Macrophages
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Silver
Adhesion
Bacteria
Biomedical equipment
Cell culture
Coatings
Implants (surgical)
Nanoparticles
antiinfective agent
interleukin 1beta
interleukin 6
silver nanoparticle
thiomalic acid
Medical Devices
animal experiment
antibacterial coating
article
bacterium culture
catheter
cell function
cell viability
cytokine release
cytotoxicity
drug coating
drug efficacy
fibroblast
human
human cell
immobilization
implant
in vitro study
innate immunity
lifespan
macrophage
medical device
mouse
nonhuman
priority journal
protein expression
surface property
wound dressing
M3 - Article
PY - 2014
SP - 4601-4609
ST - Substrate independent silver nanoparticle based antibacterial coatings
T2 - Biomaterials
TI - Substrate independent silver nanoparticle based antibacterial coatings
84896545626&doi=10.1016%2fj.biomaterials.2014.02.033&partnerID=40&md5=f8348477b5754
594980b5466f6f74650
VL - 35
ID - 5562
ER -
TY - JOUR
AB - Introduction: Silver nanoparticles have been used for different purposes in
dentistry, including endodontic treatments. The aim of this study was to determine
the cytotoxicity of different types of silver nanoparticles on mouse fibroblast
cell line L929 and the reaction of subcutaneous connective tissue of Wistar rats to
these nanoparticles. Methods: Silver nanoparticles of an average size of 5 nm were
synthesized with ammonia (SNA) or polyvinylpyrrolidone (SNP). L929 was exposed to
SNA and SNP (0.1-100 mu g/mL), and 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide and enzyme-linked immunosorbent assays were performed
after 6, 24, and 48 hours. Culture medium was used as the control. Sixteen rats
received, individually, 3 polyethylene tubes filled with a fibrin sponge embedded
in 100 mu L SNA or SNP (1 mu g/mL). A fibrin sponge with no embedding was the
control. Tissue reaction was performed qualitatively and quantitatively after 7,
15, 30, and 90 days of implantation in the dorsal connective tissue of Wistar rats.
Results: SNA and SNP were cytotoxic to L929 in higher concentrations, with SNA
significantly more toxic than SNP. SNA and SNP did not induce significant
interleukin-1 beta and interleukin-6 production. The release of stern cell factor
by L929 increased 48 hours after the treatment with SNP at 5 mu g/rnL. Histologic
examination showed that the inflammatory responses caused by SNA and SNP at 1 mu
g/mL were similar to the control in all experimental periods. Conclusions: It was
concluded that SNA and SNP were not cytotoxic at 25 mu g/mL or lower
concentrations. However, for safe clinical use, further studies establishing others
points of its toxicologic profile are recommended.
AN - WOS:000377821200019
AU - Takamiya, A. S.
AU - Monteiro, D. R.
AU - Bernabe, D. G.
AU - Gorup, L. F.
AU - Camargo, E. R.
AU - Gomes, J. E.
AU - Oliveira, S. H. P.
AU - Barbosa, D. B.
DA - JUN
DO - 10.1016/j.joen.2016.03.014
IS - 6
PY - 2016
SN - 0099-2399
1878-3554
SP - 953-960
ST - In Vitro and In Vivo Toxicity Evaluation of Colloidal Silver Nanoparticles
Used in Endodontic Treatments
TI - In Vitro and In Vivo Toxicity Evaluation of Colloidal Silver Nanoparticles
VL - 42
ID - 5856
ER -
TY - JOUR
AB - Introduction Silver nanoparticles have been used for different purposes in
dentistry, including endodontic treatments. The aim of this study was to determine
the cytotoxicity of different types of silver nanoparticles on mouse fibroblast
cell line L929 and the reaction of subcutaneous connective tissue of Wistar rats to
these nanoparticles. Methods Silver nanoparticles of an average size of 5 nm were
synthesized with ammonia (SNA) or polyvinylpyrrolidone (SNP). L929 was exposed to
SNA and SNP (0.1-100 μg/mL), and 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide and enzyme-linked immunosorbent assays were performed
after 6, 24, and 48 hours. Culture medium was used as the control. Sixteen rats
received, individually, 3 polyethylene tubes filled with a fibrin sponge embedded
in 100 μL SNA or SNP (1 μg/mL). A fibrin sponge with no embedding was the control.
Tissue reaction was performed qualitatively and quantitatively after 7, 15, 30, and
90 days of implantation in the dorsal connective tissue of Wistar rats. Results SNA
and SNP were cytotoxic to L929 in higher concentrations, with SNA significantly
more toxic than SNP. SNA and SNP did not induce significant interleukin-1β and
interleukin-6 production. The release of stem cell factor by L929 increased 48
hours after the treatment with SNP at 5 μg/mL. Histologic examination showed that
the inflammatory responses caused by SNA and SNP at 1 μg/mL were similar to the
control in all experimental periods. Conclusions It was concluded that SNA and SNP
were not cytotoxic at 25 μg/mL or lower concentrations. However, for safe clinical
use, further studies establishing others points of its toxicologic profile are
recommended. © 2016 American Association of Endodontists.
AU - Bernabé, D. G.
AU - Gorup, L. F.
AU - Camargo, E. R.
AU - Gomes-Filho, J. E.
AU - Oliveira, S. H. P.
AU - Barbosa, D. B.
DB - Scopus
DO - 10.1016/j.joen.2016.03.014
IS - 6
endodontic materials
inflammation
toxicity
Ammonia
Animals
Cell Line
Cell Survival
Connective Tissue
Dental Materials
Fibrin
Fibroblasts
Inflammation
Interleukin-1beta
Interleukin-6
Male
Materials Testing
Metal Nanoparticles
Mice
Neutrophils
Particle Size
Porifera
Povidone
Rats
Rats, Wistar
Silver
Stem Cell Factor
Subcutaneous Tissue
Tetrazolium Salts
Thiazoles
Time Factors
Toxicity Tests
ammonia
colloidal silver
dental material
fibrin
interleukin 1beta
interleukin 6
metal nanoparticle
povidone
silver
stem cell factor
tetrazolium
thiazole derivative
thiazolyl blue
animal
cell line
cell survival
chemistry
connective tissue
dose response
drug effects
fibroblast
male
materials testing
metabolism
mouse
neutrophil
particle size
pathology
procedures
rat
sponge (Porifera)
subcutaneous tissue
time factor
toxicity testing
Wistar rat
M3 - Article
PY - 2016
SP - 953-960
ST - In Vitro and in Vivo Toxicity Evaluation of Colloidal Silver Nanoparticles
TI - In Vitro and in Vivo Toxicity Evaluation of Colloidal Silver Nanoparticles
84964267455&doi=10.1016%2fj.joen.2016.03.014&partnerID=40&md5=4e3e1fe9fdd24df8da4c3
753c0024c81
VL - 42
ID - 5482
ER -
TY - JOUR
AB - Background: Although silver nanoparticles (SNP) have proven antimicrobial
activity against different types of microorganisms, the effect of SNP incorporation
into acrylic resin to control Candida albicans biofilm formation aiming at the
prevention of Candida-associated denture stomatitis has not yet been fully
elucidated. Objectives: This study aimed to evaluate the antimicrobial effect of an
acrylic resin containing SNP on C. albicans biofilm growth, the flexural strength
of this material and tissue reaction in the subcutaneous connective tissue of rats
to SNP. Method: SNP were synthesized through silver nitrate reduction by sodium
citrate. Transmission electron microscopy (TEM) and scanning electron microscopy
(SEM) were used to verify the size and colloidal stability. SNP were added to
acrylic resin monomer (Lucitone 550) at 0.05, 0.5 and 5 vol%. The antimicrobial
effect against C. albicans (ATCC 10231) was investigated by the enumeration of
colony-forming units (CFUs) and SEM. The threepoint bending test was performed to
analyze the flexural strength. Tissue reaction was evaluated after 7 and 60 days of
implantation in the connective tissue of Wistar rats. Results: Spherical particles
of 5 and 10 nm were obtained. SNP at 0.05 and 0.5% incorporated into acrylic resin
was effective in reducing C. albicans biofilm growth (p < .001). SEM revealed that
the material was able to disrupt C. albicans biofilm formation and did not reduce
the flexural strength compared to control (p > .05). The inflammatory response
observed 60 days after implantation SNP in the subcutaneous tissue was similar to
control. Conclusion: It was concluded that SNP addition at 0.05 and 0.5% into
acrylic resin exhibited antimicrobial effects against C. albicans biofilm, did not
interfere in the flexural strength and may be considered biocompatible.
AN - WOS:000600850700001
AU - Gorup, L. F.
AU - Silva, E. A.
AU - de Camargo, E. R.
AU - Gomes, J. E.
AU - de Oliveira, S. H. P.
AU - Barbosa, D. B.
C7 - 111341
DA - JAN
DO - 10.1016/j.msec.2020.111341
PY - 2021
SN - 0928-4931
1873-0191
ST - Biocompatible silver nanoparticles incorporated in acrylic resin for dental
application inhibit Candida albicans biofilm
TI - Biocompatible silver nanoparticles incorporated in acrylic resin for dental
VL - 118
ID - 5985
ER -
TY - JOUR
AB - Background: Although silver nanoparticles (SNP) have proven antimicrobial
activity against different types of microorganisms, the effect of SNP incorporation
into acrylic resin to control Candida albicans biofilm formation aiming at the
prevention of Candida-associated denture stomatitis has not yet been fully
elucidated. Objectives: This study aimed to evaluate the antimicrobial effect of an
acrylic resin containing SNP on C. albicans biofilm growth, the flexural strength
of this material and tissue reaction in the subcutaneous connective tissue of rats
to SNP. Method: SNP were synthesized through silver nitrate reduction by sodium
citrate. Transmission electron microscopy (TEM) and scanning electron microscopy
(SEM) were used to verify the size and colloidal stability. SNP were added to
acrylic resin monomer (Lucitone 550) at 0.05, 0.5 and 5 vol%. The antimicrobial
effect against C. albicans (ATCC 10231) was investigated by the enumeration of
colony-forming units (CFUs) and SEM. The three-point bending test was performed to
analyze the flexural strength. Tissue reaction was evaluated after 7 and 60 days of
implantation in the connective tissue of Wistar rats. Results: Spherical particles
of 5 and 10 nm were obtained. SNP at 0.05 and 0.5% incorporated into acrylic resin
was effective in reducing C. albicans biofilm growth (p < .001). SEM revealed that
the material was able to disrupt C. albicans biofilm formation and did not reduce
the flexural strength compared to control (p > .05). The inflammatory response
observed 60 days after implantation SNP in the subcutaneous tissue was similar to
control. Conclusion: It was concluded that SNP addition at 0.05 and 0.5% into
acrylic resin exhibited antimicrobial effects against C. albicans biofilm, did not
interfere in the flexural strength and may be considered biocompatible. © 2020
Elsevier B.V.
AU - Gorup, L. F.
AU - Silva, E. A.
AU - de Camargo, E. R.
AU - Gomes-Filho, J. E.
AU - de Oliveira, S. H. P.
AU - Barbosa, D. B.
C7 - 111341
DB - Scopus
DO - 10.1016/j.msec.2020.111341
KW - Acrylic resin
Antimicrobial
Biocompatibility
Denture stomatitis
Flexural strength
Acrylic Resins
Animals
Biofilms
Candida albicans
Denture Bases
Metal Nanoparticles
Rats
Rats, Wistar
Silver
Bending dies
Bending strength
Candida
Collagen
Metal nanoparticles
Microorganisms
Musculoskeletal system
Resins
Silver compounds
Sodium compounds
Yeast
acrylic acid resin
metal nanoparticle
silver
Colloidal Stability
Colony forming units
Dental applications
Subcutaneous tissues
Three-point bending test
animal
biofilm
denture base
rat
Wistar rat
Tissue
M3 - Article
PY - 2021
ST - Biocompatible silver nanoparticles incorporated in acrylic resin for dental
TI - Biocompatible silver nanoparticles incorporated in acrylic resin for dental
85090165855&doi=10.1016%2fj.msec.2020.111341&partnerID=40&md5=8e56732e2e11741053ae4
1a4c67368f1
VL - 118
ID - 5260
ER -
TY - GEN
AB - O objetivo deste estudo foi investigar o efeito de diferentes soluções
coloidais de nanopartículas de prata sobre a viabilidade celular de fibroblastos
(linhagem L929) e sobre a resposta inflamatória de tecido subcutâneo de ratos.
Nanopartículas de prata (SNP) com tamanho médio de 5 nm foram sintetizadas através
da redução do nitrato de prata pelo citrato de sódio e estabilizadas com amônia
(SNP-A) ou polivinilpirrolidona (SNP-P). Para avaliar a viabilidade celular,
células L929 foram expostas SNP e agentes estabilizantes (amônia (NH3) e
polivinilpirrolidona (PVP)) (0,1 100 μg/mL), e após 6, 24 e 48 h foi realizado o
ensaio de citotoxicidade celular pelo método do MTT. A resposta tecidual foi
realizada com tubos de polietileno contendo SNP (1.0 μg/mL; 540 μg/mL) e agentes
estabilizantes (NH3 0.13 x 10-3 mol/L e PVP 0.19 g/L) implantados no tecido
conjuntivo dorsal de ratos Wistar por 7, 15, 30, 60 e 90 dias. Os espécimes foram
corados com hematoxilina e eosina e foram realizadas avaliações qualitativa e
quantitativa. SNP inibiram a viabilidade celular no teste in vitro de maneira
concentração-dependente. SNP-A foram mais tóxicas para L929 que as partículas
estabilizadas com PVP. O exame histológico mostrou que SNP 540 μg/mL induziram
reação tecidual significantemente mais intensa em 30 e 60 dias comparado aos grupos
controles (solução fisiológica 0,9% e fibrina) nos mesmos períodos. As respostas
inflamatórias causadas por SNP 1,0 μg/mL, NH3 0,13 x 10- 3 mol/L e PVP 0,19 g/L
foram similares aos controles em todos os períodos experimentais. Foi possível
concluir que a exposição à SNP reduziu a viabilidade de células L929 de maneira
concentração-dependente. O tipo de agente estabilizante interferiu na
citotoxicidade sendo SNP-A mais tóxica para L929. Ambos os tipos de soluções
coloidais de nanopartículas de prata (SNP-A e SNP-P) a 540 µg/mL induziram
significante resposta inflamatória no tecido subcutâneo de rato
The aim of this study was to investigate the effect of different colloidal silver
nanoparticles on cell viability of mouse fibroblasts (cell line L929) and on the
subcutaneous connective tissue reaction of rats. Silver nanoparticles (SNP) of
average size 5 nm were synthesized by the reduction of silver nitrate through
sodium citrate and were stabilized with ammonia (SNP-A) or polyvinylpyrrolidone
(SNP-P). To evaluate the cell viability, L929 cell were exposure to silver
nanoparticles (0.1-100 μg/mL), and after 6, 24 and 48h MTT assay was performed. The
tissue reaction was carried out with polyethylene tubes containing silver
nanoparticles (1.0 μg/mL; 540 μg/mL) implanted in the dorsal connective tissue of
Wistar rats for 7, 15, 30, 60, and 90 days. The specimens were stained with
hematoxylin and eosin and qualitative and quantitative evaluations of the reaction
were carried out. Silver nanoparticles inhibited the cell viability in the in vitro
test in a concentration-dependent manner. SNP-A were more toxic to L929 than
particles stabilized with polyvinylpyrrolidone (PVP). Histological examination
showed that SNP at 540 μg/mL induced significant tissue reaction on 30 and 60 days
after implantation compared to the controls groups (fibrin and saline 0.9%) at the
same periods. The inflammatory responses caused by SNP at 1.0 μg/ml, NH3 at 0.13 x
10-3 mol/L and PVP at 0.19 g/L solutions were similar to the controls groups in all
experimental periods. It was possible to conclude that SNP exposure decreased the
viability of L929 cells in a concentration-dependent manner. The type of
stabilizing agent interfered on the cytotoxicity of SNP being SNP-A more toxic to
L929. Also, both colloidal silver nanoparticles (SNP-A and SNP-P) at 540 μg/mL
induced significant inflammatory response in rats subcutaneous tissue
AU - Takamiya, Aline Satie
C1 - 20140402
C8 - biblio-866915
DA - 2013/00
DB - LILACS
KW - Biocompatibilidade
Biocompatibility
Nanoparticle
Nanopartícula
Prata
Silver
Toxicidade
Toxicity
LA - en
PY - 2013
SP - 108-108
ST - Avaliação da resposta tecidual e da citotoxicidade de soluções coloidais de
nanopartículas de prata
TI - Avaliação da resposta tecidual e da citotoxicidade de soluções coloidais de
nanopartículas de prata
TT - Evaluation of tissue reaction and cytotoxicity of colloidal silver
nanoparticles
UR - http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/04-06-
2014/000737014.pdf
ID - 4942
ER -
TY - JOUR
AB - A new Ag(I) boron imidazolate framework with the chemical formula of
{Ag[BH(im)3]}n (1, BH(im)3− = tri(imidazolyl)borate) has been synthesized by the
self-assembly of Ag(I) and tridentate boron imidazolate ligand. This compound shows
intense luminescence and outstanding photocatalytic activity for the Rhodamine B
degradation with an irradiation of ultraviolet. Moreover, the compound’s
therapeutic effects on neonatal pneumonia were explored and the corresponding
mechanism was tested in meantime. The enzyme linked immunosorbent assay detection
was firstly conducted to measure the content of inflammatory cytokines released
into the bronchoalveolar lavage fluid. Then, the activation of JNK/MAPK signaling
pathway activation in the alveolar epithelial cells was measured with the real-time
reverse transcription-polymerase chain reaction. Finally, the cytotoxicity of the
complex was determined with Cell Counting Kit (CCK-8) assay. © 2022, The Author(s),
under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
AU - Tan, J. F.
AU - Zhao, H.
DB - Scopus
DO - 10.1007/s00289-022-04419-4
IS - 7
KW - Ag(I) compound
Cytotoxicity
Inflammatory cytokine
Neonatal pneumonia
Photocatalysis
Boron
Cell signaling
Functional polymers
Irradiation
Photocatalytic activity
Silver compounds
Chemical formulae
Coordination Polymers
Coordination-polymers
Imidazolate
Imidazolyl
Photocatalytic property
Therapeutic effects
Chemical activation
M3 - Article
PY - 2023
SP - 7779-7790
ST - Layered Ag(I) coordination polymer: photocatalytic property and therapeutic
effects on neonatal pneumonia
T2 - Polymer Bulletin
TI - Layered Ag(I) coordination polymer: photocatalytic property and therapeutic
effects on neonatal pneumonia
85136056036&doi=10.1007%2fs00289-022-04419-
4&partnerID=40&md5=188dd6717fc54511c43d43ae86e9a687
VL - 80
ID - 5026
ER -
TY - JOUR
AB - The effective treatment for periodontitis is to completely and sustainedly
eradicate the bacterial pathogens from the complex periodontal pockets. Local
sustained-release antibiotics as a complementary treatment after scaling and root
planning can sustainedly combat bacterial pathogens in the periodontal pockets to
help treat the disease, but the increasing concern of bacterial resistance limits
its future use. Here, we reported a local antibacterial system based on microsized
multifunctional Ag-TiO2-x encapsulated in alginate (ATA) microspheres. We confirmed
that ATA displayed strong photothermally enhanced dual enzyme-mimicking
(peroxidase-like and catalase-like) activities and weak photocatalytic activity
under 808 nm near-infrared (NIR) irradiation, which could boost the generation of
reactive oxygen species (ROS) and O-2 in the presence of low-level H2O2. As a
result, the ATA/H2O2/NIR system exhibited efficient antibacterial activity against
Porphyromonas gingivalis and Streptococcus gordonii in both planktonic and biofilm
forms. With the help of ROS, ATA could release Ag+ in concentrations sufficient to
inhibit periodontal pathogens as well. Moreover, the in situ-generated oxygen was
supposed to alleviate the local hypoxic environment and would help downregulate the
lipopolysaccharide-mediated inflammatory response of periodontal stem cells. The in
vivo rat periodontitis treatment results demonstrated that the ATA/H2O2/NIR system
reduced the bacterial load, relieved inflammation, and improved tissue healing. Our
work developed a new local prolonged bactericidal and oxygenation system for
enhanced periodontitis. Avoiding the usage of antibiotics and nanomaterials, this
strategy showed great promise in adjunctive periodontitis treatment and also in
other biomedical applications.
AN - WOS:000904691800001
AU - Tan, X. Z.
AU - Liu, S. R.
AU - Hu, X. Y.
AU - Zhang, R. T.
AU - Su, X. F.
AU - Qian, R. J.
AU - Mai, Y.
AU - Xu, Z. Y.
AU - Jing, W.
AU - Tian, W. D.
AU - Xie, L.
C6 - DEC 2022
DA - 2022 DEC 23
DO - 10.1021/acsami.2c17065
PY - 2022
SN - 1944-8244
1944-8252
ST - Near-Infrared-Enhanced Dual Enzyme-Mimicking Ag-TiO2-x@Alginate Microspheres
with Antibactericidal and Oxygeneration Abilities to Treat Periodontitis
TI - Near-Infrared-Enhanced Dual Enzyme-Mimicking Ag-TiO2-x@Alginate Microspheres
with Antibactericidal and Oxygeneration Abilities to Treat Periodontitis
ID - 6833
ER -
TY - JOUR
AB - Engineered nanomaterials (ENMs) have been widely exploited in several
industrial domains as well as our daily life, raising concern over their potential
adverse effects. While in general ENMs do not seem to have detrimental effects on
immunity or induce severe inflammation, their indirect effects on immunity are less
known. In particular, since the gut microbiota has been tightly associated with
human health and immunity, it is possible that ingested ENMs could affect
intestinal immunity indirectly by modulating the microbial community composition
and functions. In this perspective, we provide a few pieces of evidence and discuss
a possible link connecting ENM exposure, gut microbiota and host immune response.
Some experimental works suggest that excessive exposure to ENMs could reshape the
gut microbiota, thereby modulating the epithelium integrity and the inflammatory
state in the intestine. Within such microenvironment, numerous microbiota-derived
components, including but not limited to SCFAs and LPS, may serve as important
effectors responsible of the ENM effect on intestinal immunity. Therefore, the gut
microbiota is implicated as a crucial regulator of the intestinal immunity upon ENM
exposure. This calls for including gut microbiota analysis within future work to
assess ENM biocompatibility and immunosafety. This also calls for refinement of
future studies that should be designed more elaborately and realistically to mimic
the human exposure situation.
AN - WOS:000704148000001
AU - Tang, M. X.
AU - Li, S.
AU - Wei, L.
AU - Hou, Z. H.
AU - Qu, J.
AU - Li, L.
C7 - 684605
DA - SEP 14
DO - 10.3389/fimmu.2021.684605
PY - 2021
SN - 1664-3224
ST - Do Engineered Nanomaterials Affect Immune Responses by Interacting With Gut
Microbiota?
TI - Do Engineered Nanomaterials Affect Immune Responses by Interacting With Gut
Microbiota?
VL - 12
ID - 6808
ER -
TY - JOUR
AB - Bacterial infections have caused many human diseases, so effective treatment
of bacterial infections is an urgent problem that needs to be solved. In this work,
a multifunctional therapeutic core-satellite nanoplatform (ASAN NPs) with
antibacterial and anti-inflammatory properties was designed and synthesized. First,
mesoporous silica-coated silver nanoparticles (Ag NPs) with different sizes were
synthesized via a simplified method that improved the stability and drug-carrying
capacity of the Ag NPs. Then, the NPs were loaded with anti-inflammatory and
antibacterial naringin. The characterization results showed that the ASAN NPs were
successfully synthesized, with an average particle size of 110 +/- 15 nm. In vitro
antibacterial test results showed that the synergistic effect of Ag NPs and
naringin enhanced the antibacterial activity in a dose-dependent manner. The ASAN
NPs achieved high-efficiency antibacterial activity by increasing the permeability
of the cell membrane, destroying the integrity of the bacteria, and significantly
increasing the level of bacterial reactive oxygen species. The in vivo
antibacterial results showed that the ASAN NPs had highly effective anti-infective
and anti-inflammatory activities and did not show obvious side effects. Thus, ASAN
NPs appear to be promising antimicrobial agents with good biocompatibility, potent
antibacterial activity, and anti-inflammatory activity.
AN - WOS:000675209300011
AU - Tang, M.
AU - Wang, L. J.
AU - Zhao, D.
AU - Huang, D. W.
C7 - 055217
DA - MAY 1
DO - 10.1063/5.0050866
IS - 5
PY - 2021
SN - 2158-3226
ST - Ag@mSiO(2)@Ag core-satellite nanostructures enhance the antibacterial and
anti-inflammatory activities of naringin
T2 - AIP ADVANCES
TI - Ag@mSiO(2)@Ag core-satellite nanostructures enhance the antibacterial and
anti-inflammatory activities of naringin
VL - 11
ID - 6020
ER -
TY - JOUR
AB - Bacterial infections have caused many human diseases, so effective treatment
of bacterial infections is an urgent problem that needs to be solved. In this work,
a multifunctional therapeutic core-satellite nanoplatform (ASAN NPs) with
antibacterial and anti-inflammatory properties was designed and synthesized. First,
mesoporous silica-coated silver nanoparticles (Ag NPs) with different sizes were
synthesized via a simplified method that improved the stability and drug-carrying
capacity of the Ag NPs. Then, the NPs were loaded with anti-inflammatory and
antibacterial naringin. The characterization results showed that the ASAN NPs were
successfully synthesized, with an average particle size of 110 ± 15 nm. In vitro
antibacterial test results showed that the synergistic effect of Ag NPs and
naringin enhanced the antibacterial activity in a dose-dependent manner. The ASAN
NPs achieved high-efficiency antibacterial activity by increasing the permeability
of the cell membrane, destroying the integrity of the bacteria, and significantly
increasing the level of bacterial reactive oxygen species. The in vivo
antibacterial results showed that the ASAN NPs had highly effective anti-infective
and anti-inflammatory activities and did not show obvious side effects. Thus, ASAN
NPs appear to be promising antimicrobial agents with good biocompatibility, potent
antibacterial activity, and anti-inflammatory activity. © 2021 Author(s).
AU - Tang, M.
AU - Wang, L.
AU - Zhao, D.
AU - Huang, D.
C7 - 055217
DB - Scopus
DO - 10.1063/5.0050866
IS - 5
Biocompatibility
Cytology
Particle size
Silica
Anti-inflammatories
Antibacterial tests
Average particle size
Dose-dependent manner
Flavonoids
M3 - Article
PY - 2021
ST - Ag@mSiO2@Ag core-satellite nanostructures enhance the antibacterial and anti-
inflammatory activities of naringin
T2 - AIP Advances
TI - Ag@mSiO2@Ag core-satellite nanostructures enhance the antibacterial and anti-
inflammatory activities of naringin
85106440175&doi=10.1063%2f5.0050866&partnerID=40&md5=433b3447c9dda4a48d9ab6d96ba625
7f
VL - 11
ID - 5170
ER -
TY - JOUR
AB - Silkworm silk is among the most widely used natural fibers for textile and
biomedical applications due to its extraordinary mechanical properties and superior
biocompatibility. A number of physical and chemical processes have also been
developed to reconstruct silk into various forms or to artificially produce silk-
like materials. In addition to the direct use and the delicate replication of
silk's natural structure and properties, there is a growing interest to introduce
more new functionalities into silk while maintaining its advantageous intrinsic
properties. In this review we assess various methods and their merits to produce
functional silk, specifically those with color and luminescence, through post-
processing steps as well as biological approaches. There is a highlight on
intrinsically colored and luminescent silk produced directly from silkworms for a
wide range of applications, and a discussion on the suitable molecular properties
for being incorporated effectively into silk while it is being produced in the silk
gland. With these understanding, a new generation of silk containing various
functional materials (e.g., drugs, antibiotics and stimuli-sensitive dyes) would be
produced for novel applications such as cancer therapy with controlled release
feature, wound dressing with monitoring/sensing feature, tissue engineering
scaffolds with antibacterial, anticoagulant or anti-inflammatory feature, and many
others.
AN - WOS:000301336500002
AU - Tansil, N. C.
AU - Koh, L. D.
AU - Han, M. Y.
DA - MAR 15
DO - 10.1002/adma.201104118
IS - 11
PY - 2012
SN - 0935-9648
1521-4095
SP - 1388-1397
ST - Functional Silk: Colored and Luminescent
T2 - ADVANCED MATERIALS
TI - Functional Silk: Colored and Luminescent
VL - 24
ID - 6613
ER -
TY - JOUR
AB - Hospital acquired infections caused due to ESKAPE pathogens pose a challenge
for treatment due to their growing antimicrobial resistance. Curcuma aromatica (CA)
is traditionally known for its antibacterial, wound healing and anti-inflammatory
properties. The present study highlights the biogenic synthesis of silver
nanoparticles (CAAgNPs) capped and stabilized by the compounds from CA rhizome
extract, also further demonstrating their antibacterial, antibiofilm and
synergistic effects against multidrug-resistant (MDR) pathogens. CAAgNPs were
synthesized using aqueous rhizome extract of CA (5 mg/ml) and AgNO3 (0.8 mM)
incubated at 60°C up to 144 h. UV-vis spectroscopy, field emission scanning
electron microscopy (FESEM), transmission electron microscopy (TEM), energy
dispersive spectroscopy (EDS) and X-ray diffraction (XRD) revealed CAAgNPs with
characteristic peak at 430 nm, 13 ± 5 nm size of spherical shape, showing presence
of silver and crystalline nature, respectively. Dynamic light scattering (DLS) and
zeta potential confirmed their monodispersed nature with average diameter of 77.88
± 48.60 nm and stability. Fourier transform infrared spectroscopic (FTIR) analysis
demonstrated the presence of phenolic -OH and carbonyl groups possibly involved in
the reduction and stabilization of CAAgNPs. The minimum inhibitory concentrations
(MICs), minimum bactericidal concentrations (MBCs) and minimum biofilm inhibitory
concentrations (MBICs) of CAAgNPs against Pseudomonas aeruginosa, NCIM 5029 and
PAW1, and, Staphylococcus aureus, NCIM 5021 and S8 were in range from 8 to
128 μg/ml. Almost 50% disruption of pre-formed biofilms at concentrations 8–
1,024 μg/ml was observed. Fluorescence microscopy and FESEM analysis confirmed cell
death and disruption of pre-formed biofilms of P. aeruginosa PAW1 and S. aureus S8.
Checkerboard assay demonstrated the synergistic effect of CAAgNPs (0.125–4 μg/ml)
in combination with various antibiotics (0.063–1,024 μg/ml) against planktonic and
biofilm forms of P. aeruginosa PAW1. The study confirms the antibacterial and
antibiofilm activity of CAAgNPs alone and in combination with antibiotics against
MDR pathogens, thus, reducing the dose as well as toxicity of both. CAAgNPs have
the potential to be used in wound dressings and ointments, and to improve the
performances of medical devices and surgical implants. In vivo toxicity of CAAgNPs
however needs to be tested further using mice models. Copyright © 2022 Tawre,
Shiledar, Satpute, Ahire, Ghosh and Pardesi.
AU - Tawre, M. S.
AU - Shiledar, A.
AU - Satpute, S. K.
AU - Ahire, K.
AU - Ghosh, S.
AU - Pardesi, K.
C7 - 1029056
DB - Scopus
DO - 10.3389/fchem.2022.1029056
KW - antibiotics
biofilms
Curcuma aromatica
multidrug-resistant
synergy
M3 - Article
PY - 2022
ST - Synergistic and antibiofilm potential of Curcuma aromatica derived silver
nanoparticles in combination with antibiotics against multidrug-resistant pathogens
TI - Synergistic and antibiofilm potential of Curcuma aromatica derived silver
nanoparticles in combination with antibiotics against multidrug-resistant pathogens
85142396300&doi=10.3389%2ffchem.2022.1029056&partnerID=40&md5=082cc50d22f64c87e0d11
5b7f8306f5b
VL - 10
ID - 5050
ER -
TY - JOUR
AB - Silica nanoparticles (SiO2 NPs) are one of the most widely used engineered
nanoparticles and can been found in a wide range of consumer products. Despite
their massive global production scale, little is known about their potential
effects in the context of unintended exposure or ingestion. Using TR146 cells as an
in vitro model of the human oral buccal mucosa, the uptake, spatial intracellular
distribution, reactive oxygen species (ROS) production, inflammatory response, and
cytotoxic effects of commercial SiO2 NPs are examined. SiO2 NPs are shown to dock
and cross the cellular membrane barrier in a dose-time-dependent manner. Confocal
sectioning reveals translocation of SiO2 NPs into the cell nucleus after 12 h of
exposure. A concentration threshold of more than 500 x 10(-6) m is observed, above
which SiO2 NPs are shown to exert significant oxidative stress with concomitant
upregulation of inflammatory genes IL6 and TNFA. Further analysis of the p53
pathway and a series of apoptotic and cell cycle biomarkers reveals intracellular
accumulation of SiO2 NPs exert marginal nanotoxicity. Collectively, this study
provides important information regarding the uptake, intracellular distribution,
and potential adverse cellular effects of SiO2 NPs commonly found in consumer
products in the human oral epithelium.
AN - WOS:000327250600008
AU - Tay, C. Y.
AU - Fang, W. R.
AU - Setyawati, M. I.
AU - Sum, C. P.
AU - Xie, J. P.
AU - Ng, K. W.
AU - Chen, X. D.
AU - Hong, C. H. L.
AU - Leong, D. T.
DA - SEP
DO - 10.1002/ppsc.201300111
IS - 9
PY - 2013
SN - 0934-0866
1521-4117
SP - 784-793
ST - Reciprocal Response of Human Oral Epithelial Cells to Internalized Silica
Nanoparticles
T2 - PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
TI - Reciprocal Response of Human Oral Epithelial Cells to Internalized Silica
Nanoparticles
VL - 30
ID - 6614
ER -
TY - JOUR
AB - Polyphenols are a broad group of bioactive phytochemicals with powerful
antioxidant, anti-inflammatory, immunomodulatory, and antiviral activities.
Numerous studies have demonstrated that polyphenol extracts obtained from natural
sources can be used for the prevention and treatment of cancer. Pomegranate peel
extract is an excellent source of polyphenols, such as punicalagin, punicalin,
ellagic acid, and caffeic acid, among others. These phenolic compounds have
antineoplastic activity in in vitro models of cervical cancer through the
regulation of cellular redox balance, induction of apoptosis, cell cycle arrest,
and modulation of different signaling pathways. The current review summarizes
recent data from scientific reports that address the anticancer activity of the
predominant polyphenol compounds present in PPE and their different mechanisms of
action in cervical cancer models.
AN - WOS:000914226400001
AU - Teniente, S. L.
AU - Flores-Gallegos, A. C.
AU - Esparza-Gonzalez, S. C.
AU - Campos-Muzquiz, L. G.
AU - Nery-Flores, S. D.
AU - Rodriguez-Herrera, R.
C7 - 127
DA - JAN
DO - 10.3390/antiox12010127
IS - 1
PY - 2023
SN - 2076-3921
ST - Anticancer Effect of Pomegranate Peel Polyphenols against Cervical Cancer
T2 - ANTIOXIDANTS
TI - Anticancer Effect of Pomegranate Peel Polyphenols against Cervical Cancer
VL - 12
ID - 6437
ER -
TY - JOUR
AB - BACKGROUND: Postconditioning (PostC) with mild hypoxia shortly after a
neonatal hypoxic-ischemic (HI) brain injury can reduce brain damage, however, the
mechanisms underlying this protection are not known. We hypothesize that hypoxic
PostC reduces brain markers of glial activity, inflammation, and apoptosis
following HI injury. METHODS: Sprague Dawley rat pups were exposed to right common
carotid artery occlusion and hypoxia (7% oxygen, 3 h) on postnatal day 7 and 24h
later, pups were exposed to hypoxic PostC (8% O-2 for 1 h/day for 5 d) or kept at
ambient conditions for the same duration. HI+N pups demonstrated similar to 10%
loss in ipsilateral brain tissue which was rescued with HI+PostC.To investigate the
cellular responses, markers of astrocytes, microglia, inflammation, and caspase 3
activity were examined using immunohistochemistry and enzyme-linked immunosorbent
assay. RESULTS: PostC reduced the area of astrocyte staining compared to HI+N.
There was also a shift in microglial morphology toward a primed state in both PostC
groups. Protein levels of interleukin-1 beta and caspase 3 were elevated in HI+N
brains and reduced by PostC. CONCLUSION: This is the first demonstration that PostC
can reduce glial activity, inflammatory mediators, and cell death after a neonatal
HI brain injury.
AN - WOS:000354755000005
AU - Teo, J. D.
AU - Morris, M. J.
AU - Jones, N. M.
DA - JUN
DO - 10.1038/pr.2015.47
IS - 6
PY - 2015
SN - 0031-3998
1530-0447
SP - 757-764
ST - Hypoxic postconditioning reduces microglial activation, astrocyte and caspase
activity, and inflammatory markers after hypoxia-ischemia in the neonatal rat brain
T2 - PEDIATRIC RESEARCH
TI - Hypoxic postconditioning reduces microglial activation, astrocyte and caspase
activity, and inflammatory markers after hypoxia-ischemia in the neonatal rat brain
VL - 77
ID - 6500
ER -
TY - JOUR
AB - Background: Phyto, or plant-derived metal nanoparticles, are an interesting
and inten-sive studied group of green synthesized nanoparticles. In the last
decade, numerous medicinal plant extracts were used for the synthesis of stable
gold or silver nanoparticles with diverse biological ef-fects, such as antioxidant
activity, antimicrobial activity, anti-inflammatory activity, hypoglycemic effect,
antitumor activity and catalytic activity. Results: This review has systematized
and discussed information from the last 5 years about the research regarding
antitumor/anticancer potential of gold nanoparticles obtained via medicinal plant
extracts, with special attention on their selective cytotoxicity on tumor cells and
on their mech-anism of action, in vitro and in vivo assessments. Conclusion: Much
more in vivo and clinical studies are needed before considering phyto-synthe-sized
gold nanoparticles as significant for future medicine. © 2021 Bentham Science
Publishers.
AU - Teodor, E. D.
AU - Radu, G. L.
DB - Scopus
DO - 10.2174/2211738508999201123213504
IS - 1
KW - Antitumor activity
Apoptosis
Cytotoxicity
Gold nanoparticles
Green synthesis
Mech-anism of action
Medicinal plant extracts
anacardic acid
Bauhinia purpurea extract
Catharanthus roseus extract
doxorubicin
flower extract
ginseng extract
Glycyrrhiza uralensis root
gold nanoparticle
peppermint
plant extract
Abutilon indicum
cashew nut
Cassia roxburghii
Catharanthus roseus
colon cancer
cytotoxicity
drug mechanism
drug synthesis
electron diffraction
Eleutherococcus senticosus
flow cytometry
ginseng
Glycyrrhiza uralensis
human
in vitro study
Indigofera
Indigofera tinctoria
Isodon rubescens
medicinal plant
Mentha piperita
MTT assay
nonhuman
osteosarcoma
particle size
Peltophorum pterocarpum
Portulaca grandiflora
Review
spinach
thermogravimetry
tumor cell
tumor growth
X ray diffraction
Zataria multiflora
zebra fish
M3 - Review
PY - 2021
SP - 51-60
ST - Phyto-synthesized gold nanoparticles as antitumor agents
T2 - Pharmaceutical Nanotechnology
TI - Phyto-synthesized gold nanoparticles as antitumor agents
85109917621&doi=10.2174%2f2211738508999201123213504&partnerID=40&md5=5162390f5e2a9b
651a1c0eb4e8498dc7
VL - 9
ID - 5199
ER -
TY - JOUR
AB - One of the major limitations of balloon angioplasty is early restenosis as a
result of elastic recoil leading to vessel occlusion. The constrictive (negative)
remodeling of the blood vessel is overcome by implanting a balloon expandible,
metal stent to dilate the artery and thereby prevent elastic recoil. However, bare
metal stent implants cause mechanical injury to the intima and release of
inflammatory mediators which then initiates formation of neointimal layer leading
to restenosis. In-stent restenosis is histologically distinct from restenosis
following balloon angioplasty, in which in-stent restenosis is accompanied by
increased smooth muscle proliferation, migration, extracellular matrix and collagen
synthesis leading to neointimal hyperplasia. To overcome neointimal hyperplasia,
stents have been coated with pharmacological agents that inhibit smooth muscle cell
proliferation and migration. The drug and polymer combination coated onto stent
device is an efficient form of drug delivery system which can provide high
concentrations of drug in the tissues over an extended period of time to achieve
antiproliferative therapeutic effect. The permanent stent implants pose the risk of
a continuous interaction between the non-biodegradable polymer coating and intimal
surface leading to physical irritation, endothelial dysfunction, hypersensitivity
reactions, delayed healing and excess risk of late stent thrombosis. This review
highlights the relationship between local drug delivery using the stent platform,
release kinetics and local vascular toxicity. Published by Elsevier Ireland Ltd.
AN - WOS:000244059300001
AU - Tesfamariam, B.
DA - JAN 30
DO - 10.1016/j.toxlet.2006.11.013
IS - 2
PY - 2007
SN - 0378-4274
1879-3169
SP - 93-102
ST - Local vascular toxicokinetics of stent-based drug delivery
TI - Local vascular toxicokinetics of stent-based drug delivery
VL - 168
ID - 6633
ER -
TY - JOUR
AB - Endothelial cell dysfunction is considered to be an early event which
subsequently leads to vascular wall disorders. Ultrastructural studies indicate
that the endothelial cell changes involve membrane damage, increased permeability,
swelling and necrosis. The endothelial cell loss of function could be as a result
of changes in hemodynamic forces (shear and/or hoop stress), direct drug-induced
cytotoxicity, mechanical device implant-induced injury and/or immune-mediated
mechanisms. Drugs may perturb endothelial cell integrity by directly triggering
inflammatory signaling cascades, enhancing expression of cellular adhesion
molecules, activation of cytotoxic T cells and/or autoantibodies directed against
endothelial cell membranes. Local release of inflammatory cytokines and chemokines
activate endothelial cells to upregulate soluble adhesion molecules, activate
neutrophils and generate reactive oxygen species which serve to amplify the initial
inflammation leading to dysregulated apoptosis, secondary necrosis and overt
vascular injury lesions. Considering the role of the endothelium in the initiation
and propagation of vascular wall injury, there is a need for the discovery of
validated biomarkers to serve as a predictor of activation of inflammatory cascades
in the development of vascular injury. This article reviews some aspects of the
multifaceted mechanisms that lead to the initial endothelial cell disruption and
subsequent vascular wall injury. (c) 2007 Elsevier Inc. All rights reserved.
AN - WOS:000244401700001
AU - DeFelice, A. F.
DA - APR
DO - 10.1016/j.vph.2006.11.005
IS - 4
PY - 2007
SN - 1537-1891
1879-3649
SP - 229-237
ST - Endothelial injury in the initiation and progression of vascular disorders
T2 - VASCULAR PHARMACOLOGY
TI - Endothelial injury in the initiation and progression of vascular disorders
VL - 46
ID - 6622
ER -
TY - JOUR
AB - The Boswellic acids are pentacyclic triterpenoids obtained from the
plant Boswellia serrata gum resin proven to be effective as anti-inflammatory
agents in the treatment of inflammatory bowel disease, rheumatoid arthritis, and
gout. The Boswellic acids were isolated from the gum resin of the plant B.
serrata and characterized by UV-HPLC, TLC, and FTIR studies and further converted
into silver nanoparticles using concentration variation method. The green
synthesized boswellic acid silver nanoparticles were novel structures with 277.3 ±
0.5 nm size, stable without agglomeration characterized by UV-HPLC, DLS, SEM, XRD,
and FTIR spectral studies. The green synthesized boswellic acid silver
nanoparticles were evaluated for in vivo anti-inflammatory activity using the
carrageenan-induced rat paw edema method and also compared with native boswellic
acid and standard diclofenac. The Boswellic acid silver nanoparticles were found to
show high inhibitory activity at 500 mg/kg and 2000 mg/kg body weight dose when
compared to the native boswellic acid and standard diclofenac at the same doses.
The novel Boswellic acid silver nanoparticles were studied for in vitro drug
release kinetics using USP dissolution apparatus I (Basket type) which resulted in
sustained release characteristics when compared with native boswellic acid. The
invivo and invitro correlation for bioavailability and size modulation impact are
needed to establish safety of Boswellic acid silver nanoparticles as novel dosage
forms. © 2022, The Author(s), under exclusive licence to Springer Science+Business
Media, LLC, part of Springer Nature.
AU - Thakur, S.
AU - Mohan, G. K.
DB - Scopus
DO - 10.1007/s12668-022-00962-6
IS - 2
KW - Boswellic acids
Carrageenan induced rat paw edema method
Green synthesis
In vitro drug release kinetics
Inflammation
Biochemistry
Diseases
Drug products
Kinetics
Rats
Resins
Spectroscopic analysis
acetonitrile
boswellic acid
buffer
carrageenan
diclofenac
distilled water
hexane
methanol
silica gel
silver nanoparticle
silver nitrate
water
Carrageenans
Drug release kinetics
In vitro drug release kinetic
In-vitro
In-vivo
Vitro drug release
acute toxicity
animal experiment
animal model
animal tissue
Article
bioavailability
Boswellia serrata
column chromatography
coma
controlled study
convulsion
diarrhea
drug release
drug safety
lacrimation
lethargy
light dark cycle
mortality
nonhuman
paw edema
physical chemistry
plethysmography
rat
salivation
Soxhlet extraction
thin layer chromatography
tremor
ultrasound
X ray diffraction
Metal nanoparticles
M3 - Article
PY - 2022
SP - 670-684
ST - In Vivo Antiinflammatory Activity of Facile Boswellic Acid Silver
Nanoparticles and In Vitro Drug Release Kinetics
T2 - BioNanoScience
TI - In Vivo Antiinflammatory Activity of Facile Boswellic Acid Silver
Nanoparticles and In Vitro Drug Release Kinetics
85127259770&doi=10.1007%2fs12668-022-00962-
6&partnerID=40&md5=8baf038201774f7b92a8447de078daa9
VL - 12
ID - 5100
ER -
TY - JOUR
AB - Silver nanowires (AgNWs) are increasingly being used in the production of
optoelectronic devices, with manufacturing processes posing a risk for occupational
exposures via inhalation. Although some studies have explored the environmental
effects of AgNWs, few data exist on human health effects. Alveolar macrophages are
central in the clearance of inhaled fibers from the lungs, with frustrated
phagocytosis often stated as a key determinant for the onset of inflammatory
reactions. However, the mechanisms through which fully ingested AgNWs interact
with, degrade, and transform within primary macrophages over time, and whether the
reactivity of the AgNWs arises due to ionic or particulate effects, or both, are
poorly understood. Here, a combination of elemental quantification, 3D tomography,
analytical transmission electron microscopy (TEM), and confocal microscopy were
employed to monitor the uptake, intracellular Ag+ availability, and processing of
AgNWs of two different lengths (1 and 10 μm) inside human monocyte-derived
macrophages (HMMs). Using AgNO3 and spherical silver nanoparticles (AgNPs) as a
comparison, the amount of total bioavailable/intracellular Ag highly correlated to
the cytotoxicity of AgNWs. The 10 μm AgNWs were completely internalized in HMMs,
with numerous lysosomal vesicles observed in close vicinity to the AgNWs. Following
cellular uptake, AgNWs dissolved and transformed intracellularly, with
precipitation of AgCl as well as Ag2S. These transformation processes were likely
due to AgNW degradation in the acidic environment of lysosomes, leading to the
release of Ag+ ions that rapidly react with Cl- and SH- species of the cell
microenvironment. Our data suggest that, in HMMs, not only frustrated phagocytosis
but also the extent of intracellular uptake and dissolution of AgNWs dictates their
cytotoxicity. © 2017 American Chemical Society.
AU - Müller, K. H.
AU - Chen, S.
AU - Goode, A. E.
AU - Yufit, V.
AU - Ryan, M. P.
AU - Porter, A. E.
DB - Scopus
IS - 10
KW - 3D tomography
analytical electron microscopy
biotransformation
macrophages
silver nanowires
Antigen-antibody reactions
Body fluids
Cytotoxicity
Electron microscopy
Enzyme activity
Immunology
Macrophages
Nanowires
Optoelectronic devices
Tomography
silver nanoparticle
3-d tomographies
Analytical electron microscopy
Analytical transmission electron microscopies (TEM)
Human monocyte-derived macrophages
Silver nanowires
Spherical silver nanoparticles
Transformation process
adherent cell
Article
cell viability
confocal microscopy
controlled study
cytotoxicity
human
human cell
in vitro study
lysosome
macrophage
monocyte
physical chemistry
priority journal
synthesis
Silver
M3 - Article
PY - 2017
SP - 2336-2347
ST - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
Cells: Intracellular Availability of Silver Governs Their Cytotoxicity
TI - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
85030766819&doi=10.1021%2facsbiomaterials.7b00479&partnerID=40&md5=400d8b2ce8145cf9
e308ee51fc17e9f5
VL - 3
ID - 5524
ER -
TY - JOUR
AB - Silver nanowires (AgNWs) are increasingly being used in the production of
optoelectronic devices, with manufacturing processes posing a risk for occupational
exposures via inhalation. Although some studies have explored the environmental
effects of AgNWs, few data exist on human health effects. Alveolar macrophages are
central in the clearance of inhaled fibers from the lungs, with frustrated
phagocytosis often stated as a key determinant for the onset of inflammatory
reactions. However, the mechanisms through which fully ingested AgNWs interact
with, degrade, and transform within primary macrophages over time, and whether the
reactivity of the AgNWs arises due to ionic or particulate effects, or both, are
poorly understood. Here, a combination of elemental quantification, 3D tomography,
analytical transmission electron microscopy (TEM), and confocal microscopy were
employed to monitor the uptake, intracellular Ag+ availability, and processing of
AgNWs of two different lengths (1 and 10 mu m) inside human monocyte-derived
macrophages (HMMs). Using AgNO3 and spherical silver nanoparticles (AgNPs) as a
comparison, the amount of total bioavailable/intracellular Ag highly correlated to
the cytotoxicity of AgNWs. The 10 mu m AgNWs were completely internalized in HMMs,
with numerous lysosomal vesicles observed in close vicinity to the AgNWs. Following
cellular uptake, AgNWs dissolved and transformed intracellularly, with
precipitation of AgCI as well as Ag2S. These transformation processes were likely
due to AgNW degradation in the acidic environment of lysosomes, leading to the
release of Ag+ ions that rapidly react with Cl- and SH- species of the cell
microenvironment. Our data suggest that, in HMMs, not only frustrated phagocytosis
but also the extent of intracellular uptake and dissolution of AgN'vVs dictates
their cytotoxicity.
AN - WOS:000412866900014
AU - Muller, K. H.
AU - Chen, S.
AU - Goode, A. E.
AU - Yufit, V.
AU - Ryan, M. P.
AU - Porter, A. E.
DA - OCT
IS - 10
PY - 2017
SN - 2373-9878
SP - 2336-2347
ST - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
TI - Silver Nanowire Particle Reactivity with Human Monocyte-Derived Macrophage
VL - 3
ID - 6107
ER -
TY - JOUR
AB - Chronic wounds often exist in a heightened state of inflammation whereby
excessive inflammatory cells release high levels of proteases and reactive oxygen
species (ROS). While low levels of ROS play a fundamental role in the regulation of
normal wound healing, their levels need to be tightly regulated to prevent a
hostile wound environment resulting from excessive levels of ROS. infection
amplifies the inflammatory response, augmenting levels of ROS which creates
additional tissue damage that supports microbial growth. Antimicrobial dressings
are used to combat infection; however, the effects of these dressing on the wound
environment and healing independent of infection are rarely assessed. Cytotoxic or
adverse effects on healing may exacerbate the hostile wound environment and prolong
healing. Here we assessed the effect on healing independent of infection of silver
oxysalts which produce higher oxidative states of silver (Ag2+/Ag3+). Silver
oxysalts had no adverse effect on fibroblast scratch wound closure whilst
significantly promoting closure of keratinocyte scratch wounds (34% increase
compared with control). Furthermore, dressings containing silver oxysalts
accelerated healing of full-thickness incisional wounds in wild-type mice, reducing
wound area, promoting reepithelialization, and dampening inflammation. We explored
the mechanisms by which silver oxysalts promote healing and found that unlike other
silver dressings tested, silver oxysalt dressings catalyze the breakdown of
hydrogen peroxide to water and oxygen. In addition, we found that silver oxysalts
directly released oxygen when exposed to water. Collectively, these data provide
the first indication that silver oxysalts promote healing independent of infection
and may regulate oxidative stress within a wound through catalysis of hydrogen
peroxide.
AN - WOS:000448193100167
AU - Thomason, H. A.
AU - Lovett, J. M.
AU - Spina, C. J.
AU - Stephenson, C.
AU - McBain, A. J.
AU - Hardman, M. J.
DA - MAR-APR
DO - 10.1111/wrr.12627
IS - 2
PY - 2018
SN - 1067-1927
1524-475X
SP - 144-152
ST - Silver oxysalts promote cutaneous wound healing independent of infection
T2 - WOUND REPAIR AND REGENERATION
TI - Silver oxysalts promote cutaneous wound healing independent of infection
VL - 26
ID - 6431
ER -
TY - JOUR
AB - With great interest, we read the paper “Chitosan nano-vehicles as
biocompatible delivering tools for a new Ag(I)curcuminoid-Gboxin analog complex in
cancer and inflammation therapy” published in the International Journal of
Biological Macromolecules. In Elbehairi's report, the human breast carcinoma line
MCF-7 were used in the in vitro cytotoxic analysis test of the curcuminoid
conjugate and its silver(I) complex. They found that Ag(I)FLLL49-GbA could induce
highly significant up-regulation of caspase-3, tumor suppressor proteins P53, and
Bax after the 48 h of treatment in MCF-7 cells (Fig. 9), and the expression of
caspase-3 was analyzed by western blot assay. However, it's well known that the
MCF-7 breast carcinoma line do not express caspase-3. The lack of caspase-3 in MCF-
7 cells is caused by a 47-base pair deletion within exon 3 of the CASP-3 gene
resulting in the skipping of this exon during pre-mRNA splicing and introduction of
a premature stop codon at position 42 that completely abrogates translation of the
CASP-3 mRNA. Therefore, the detection of a caspase-3 protein in caspase-3 deficient
MCF-7 cells in this study made us confused. We appreciate the authors' efforts in
investigating the effects of chitosan nano-vehicles as biocompatible delivering
tools for a new Ag(I)curcuminoid-Gboxin analog complex in cancer and inflammation
therapy. Nevertheless, we sincerely suggest that appropriate modification may
further solidify the findings of the study. © 2023
AU - Tian, T.
C7 - 123310
DB - Scopus
DO - 10.1016/j.ijbiomac.2023.123310
KW - Ag(I)-curcuminoid macromolecule-loaded chitosan nano-vehicles
Anticancer and anti-inflammatory
Caspase-3
MCF-7 cells
Apoptosis
Breast Neoplasms
Caspase 3
Caspase 9
Chitosan
Female
Humans
MCF-7 Cells
caspase 3
caspase 6
curcumin
protein p53
silver
tumor suppressor protein
caspase 9
chitosan
apoptosis
Article
breast carcinoma
cytotoxicity
human
human cell
inflammation
MCF-7 cell line
RNA splicing
upregulation
Western blotting
breast tumor
female
metabolism
pathology
M3 - Article
PY - 2023
ST - MCF-7 cells lack the expression of Caspase-3
TI - MCF-7 cells lack the expression of Caspase-3
85146860698&doi=10.1016%2fj.ijbiomac.2023.123310&partnerID=40&md5=04bebd127ce3db2a6
0dbd91abc954d1f
VL - 231
ID - 5028
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials.
Following oral exposure, AgNPs can accumulate in various organs including kidneys
where they show gender specific accumulation. There is limited information on their
effect on renal system following long-term animal exposure especially at the
ultramicroscopic and molecular level. In this study, we have assessed the effect of
60 days oral AgNPs treatment on kidneys of female Wistar rats at doses of 50 ppm
and 200 ppm that are below previously reported lowest observed adverse effect level
(LOAEL). AgNPs treatment led to decrease in kidney weight and some loss of renal
function as seen by increased levels of serum creatinine and early toxicity markers
such as KIM-1, clusterin and osteopontin. We also observed significant
mitochondrial damage, loss of brush border membranes, pronounced swelling of
podocytes and degeneration of their foot processes using transmission electron
microscopy (TEM). These symptoms are similar to those seen in nephrotic syndrome
and ‘Minimal change disease’ of kidney where few changes are visible under light
microscopy but significant ultrastructural damage is observed. Prolonged treatment
of AgNPs also led to the activation of cell proliferative, survival and
proinflammatory factors (Akt/mTOR, JNK/Stat and Erk/NF-κB pathways and IL1β, MIP2,
IFN-γ, TNF-α and RANTES) and dysfunction of normal apoptotic pathway. Our study
shows how long term AgNPs exposure may promote ultrastructural damage to kidney
causing inflammation and expression of cell survival factors. These changes, in the
long term, could lead to inhibition of the beneficial apoptotic pathway and
promotion of necrotic cell death in kidneys. © 2017 Informa UK Limited, trading as
AU - Tiwari, R.
AU - Singh, R. D.
AU - Khan, H.
AU - Gangopadhyay, S.
AU - Mittal, S.
AU - Singh, V.
AU - Arjaria, N.
AU - Shankar, J.
AU - Roy, S. K.
AU - Singh, D.
AU - Srivastava, V.
DB - Scopus
DO - 10.1080/17435390.2017.1343874
IS - 5
KW - growth factor signaling
inflammation
kidney
Nanosilver
necrosis
nephrotoxicity
Animals
Apoptosis
Female
Kidney
Metal Nanoparticles
Necrosis
Rats
Rats, Wistar
Silver
Toxicity Tests, Subchronic
clusterin
CXCL3 chemokine
gamma interferon
interleukin 12p40
interleukin 17
interleukin 18
interleukin 1alpha
interleukin 1beta
interleukin 6
interleukin 7
kidney injury molecule 1
mammalian target of rapamycin
osteopontin
protein kinase B
RANTES
silver nanoparticle
STAT protein
tissue inhibitor of metalloproteinase 1
vasculotropin
metal nanoparticle
silver
animal experiment
animal model
animal tissue
apoptosis
Article
brush border
cell death
cell proliferation
cell survival
cell swelling
cell ultrastructure
controlled study
DNA damage
female
glomerulus basement membrane
kidney dysfunction
kidney injury
kidney mass
long term exposure
microscopy
minimal change glomerulonephritis
nanotoxicology
nephrotic syndrome
nonhuman
oxidative stress
podocyte
priority journal
protein expression
rat
Wistar rat
animal
chemically induced
cytology
drug effects
pathology
pathophysiology
toxicity testing
M3 - Article
PY - 2017
SP - 671-686
ST - Oral subchronic exposure to silver nanoparticles causes renal damage through
apoptotic impairment and necrotic cell death
T2 - Nanotoxicology
TI - Oral subchronic exposure to silver nanoparticles causes renal damage through
apoptotic impairment and necrotic cell death
85021803129&doi=10.1080%2f17435390.2017.1343874&partnerID=40&md5=a5195a7e97a86f18a1
e290b592f35df4
VL - 11
ID - 5526
ER -
TY - JOUR
AB - Chronic, multifactorial illnesses of the gastrointestinal tract include
inflammatory bowel diseases. One of the greatest methods for regulated medicine
administration in a particular region of inflammation is the nanoparticle system.
Silver nanoparticles (Ag NPs) have been utilized as drug delivery systems in the
pharmaceutical industry. The goal of the current study is to synthesize drug-loaded
Ag NPs using a previously described 3-methyl-1-phenylbutan-2-amine, as a mebeverine
precursor (MP). Methods: A green, galactose-assisted method for the rapid synthesis
and stabilization of Ag NPs as a drug-delivery system is presented. Galactose was
used as a reducing and capping agent forming a thin layer encasing the
nanoparticles. Results: The structure, size distribution, zeta potential, surface
charge, and the role of the capping agent of drug-loaded Ag NPs were discussed. The
drug release of the MP-loaded Ag NPs was also investigated. The Ag NPs indicated a
very good drug release between 80 and 85%. Based on the preliminary results, Ag NPs
might be a promising medication delivery system for MP and a useful treatment
option for inflammatory bowel disease. Therefore, future research into the
potential medical applications of the produced Ag NPs is necessary.
AN - WOS:001016921700001
AU - Todorova, M.
AU - Milusheva, M.
AU - Kaynarova, L.
AU - Georgieva, D.
AU - Delchev, V.
AU - Simeonova, S.
AU - Pilicheva, B.
AU - Nikolova, S.
C7 - 1593
DA - JUN
IS - 6
PY - 2023
SN - 2227-9059
ST - Drug-Loaded Silver Nanoparticles-A Tool for Delivery of a Mebeverine
Precursor in Inflammatory Bowel Diseases Treatment
T2 - BIOMEDICINES
TI - Drug-Loaded Silver Nanoparticles-A Tool for Delivery of a Mebeverine
Precursor in Inflammatory Bowel Diseases Treatment
VL - 11
ID - 5961
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) are commonly utilized in medicine. However, they
have negative effects on the majority of organs, including the reproductive system.
AgNPs were reported to be able to reach the testicular tissues due to their nano
size, which allows them to pass through blood-testicular barriers. The goal of this
study was to see if alpha-lipoic acid (LA) or Ginkgo biloba (GB) might protect
adult rat testes after intraperitoneal injection of AgNPs. Forty male healthy adult
Wister albino rats were randomly assigned to four groups: control, AgNPs-
intoxicated group intraperitoneally injected AgNPs 50 mg/kg b.w, 3 times a week; LA
+ AgNPs group intoxicated with AgNPs and orally gavaged with 100 mg LA/kg b.w; and
GB + AgNPs group injected with AgNPs and orally given GB extract 120 mg/kg b.w for
30 consecutive days. Biochemical changes (testosterone, ACP, and prostatic acid
phosphatase), oxidative indices, mRNA expression of proapoptotic (BAX) and anti-
apoptotic (BCL-2) biomarkers, histological, and immunohistochemical changes in
testicular tissues were investigated. Significant decrease in serum testosterone
level and elevation in ACP and PACP enzyme activity in AgNPs-treated rats. As well,
there were lowering in tGSH, GSH GR, GPx, and elevation in MDA and GSSG values.
AgNPs-exposed rats expressed downregulation of testicular thirodexin-1 (Txn-1),
transforming growth factor-1β (TGF-1β), anti-apoptotic (BCL-2), and upregulaion of
proapoptotic biomarkers (BAX) mRNA expressions. Strong positive action to BAX and
lowering the action of Ki-67 antibody were observed. Because of their antioxidant,
anti-inflammatory, and anti-apoptotic properties, cotreatment with LA or GB could
be beneficial in reducing the harmful effects of AgNPs on the testicles. © 2022,
The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of
Springer Nature.
AU - Tohamy, H. G.
AU - Lebda, M. A.
AU - Sadek, K. M.
AU - Elfeky, M. S.
AU - Samak, D. H.
AU - Hamed, H. S.
AU - Abouzed, T. K.
DB - Scopus
DO - 10.1007/s11356-021-18441-y
IS - 25
KW - Alpha-lipoic acid
Apoptosis
Bax
Ginkgo biloba
KI-67
Silver nanoparticle
Animals
Antioxidants
Biomarkers
Humans
Male
Metal Nanoparticles
Oxidative Stress
Plant Extracts
Rats
Rats, Wistar
RNA, Messenger
Silver
Testicular Diseases
Testosterone
Thioctic Acid
antioxidant
biological marker
messenger RNA
metal nanoparticle
plant extract
protein Bax
protein bcl 2
silver
testosterone
thioctic acid
antibody
apoptosis
biochemistry
cytology
enzyme
enzyme activity
growth
induced response
nanoparticle
rodent
animal
chemistry
human
male
metabolism
oxidative stress
rat
testis disease
Wistar rat
M3 - Article
PY - 2022
SP - 38198-38211
ST - Biochemical, molecular and cytological impacts of alpha-lipoic acid and
Ginkgo biloba in ameliorating testicular dysfunctions induced by silver
nanoparticles in rats
TI - Biochemical, molecular and cytological impacts of alpha-lipoic acid and
Ginkgo biloba in ameliorating testicular dysfunctions induced by silver
nanoparticles in rats
85123488602&doi=10.1007%2fs11356-021-18441-
y&partnerID=40&md5=34acea64b29ffe7bce11f676ca66621a
VL - 29
ID - 5125
ER -
TY - JOUR
AB - This work determines whether cytokine-induced neutrophil chemoattractants
(CINC)-1, CINC-2 and CINC-3 can be markers for predicting high or low pulmonary
toxicity of nanomaterials (NMs). We classified NMs of nickel oxide (NiO) and cerium
dioxide (CeO2) into high toxicity and NMs of two types of titanium dioxides
(TiO2(P90 and rutile)) and zinc oxide (ZnO) into low toxicity, and we analyzed
previous data of CINCs in bronchoalveolar lavage fluid (BALF) of rats from three
days to six months after intratracheal instillation (0.2 and 1.0 mg) and inhalation
exposure (0.32-10.4 mg/m(3)) of materials (NiO, CeO2, TiO2(P90 and rutile), ZnO NMs
and micron-particles of crystalline silica (SiO2)). The concentration of CINC-1 and
CINC-2 in BALF had different increase tendency between high and low pulmonary
toxicity of NMs and correlated with the other inflammatory markers in BALF.
However, CINC-3 increased only slightly in a dose-dependent manner compared with
CINC-1 and CINC-2. Analysis of receiver operating characteristics for the toxicity
of NMs by CINC-1 and CINC-2 showed the most accuracy of discrimination of the
toxicity at one week or one month after exposure and CINC-1 and CINC-2 in BALF
following intratracheal instillation of SiO(2)as a high toxicity could accurately
predict the toxicity at more than one month after exposure. These data suggest that
CINC-1 and CINC-2 may be useful biomarkers for the prediction of pulmonary toxicity
of NMs relatively early in both intratracheal instillation and inhalation exposure.
AN - WOS:000568070200001
AU - Tomonaga, T.
AU - Izumi, H.
AU - Oyabu, T.
AU - Lee, B. W.
AU - Kubo, M.
AU - Shimada, M.
AU - Noguchi, S.
AU - Nishida, C.
AU - Yatera, K.
AU - Morimoto, Y.
C7 - 1563
DA - AUG
DO - 10.3390/nano10081563
IS - 8
PY - 2020
SN - 2079-4991
ST - Assessment of Cytokine-Induced Neutrophil Chemoattractants as Biomarkers for
Prediction of Pulmonary Toxicity of Nanomaterials
T2 - NANOMATERIALS
TI - Assessment of Cytokine-Induced Neutrophil Chemoattractants as Biomarkers for
Prediction of Pulmonary Toxicity of Nanomaterials
VL - 10
ID - 6470
ER -
TY - JOUR
AB - The ever-growing threats of multidrug-resistant bacterial infection and
chronic wound healing have created an imperative need for the development of novel
antibacterial materials and therapeutic strategies, especially for diabetic
patients infected with multidrug-resistant bacteria. In this work, the
nanocomplexes named as PB@ PDA@Ag were used for eradicating multidrug-resistant
bacteria and accelerating wound healing of MRSA-infected diabetic model with the
assistance of laser irradiation. In vitro results revealed that the combinational
strategy exerted a synergistic effect for anti-MRSA through disrupting cell
integrity, producing ROS, declining ATP, and oxidizing GSH, comparing with
PB@PDA@Ag or NIR laser irradiation alone. Moreover, in vivo assay demonstrated that
this system effectively accelerated MRSA-infected diabetic wound healing by
mitigating local inflammatory response and up-regulating VEGF expression on the
wound bed. Meanwhile, satisfactory biocompatibility and negligible damage to major
organs were observed. Altogether, the aforementioned results indicate that the
combinational therapy of PB@PDA@Ag and NIR irradiation shows a great potential
application in the field of clinic infection.
AN - WOS:000523565800006
AU - Tong, C. Y.
AU - Zhong, X. H.
AU - Yang, Y. J.
AU - Liu, X.
AU - Zhong, G. W.
AU - Xiao, C.
AU - Liu, B.
AU - Wang, W.
AU - Yang, X. P.
C7 - 119936
DA - JUN
PY - 2020
SN - 0142-9612
1878-5905
ST - PB@PDA@Ag nanosystem for synergistically eradicating MRSA and accelerating
diabetic wound healing assisted with laser irradiation
T2 - BIOMATERIALS
TI - PB@PDA@Ag nanosystem for synergistically eradicating MRSA and accelerating
VL - 243
ID - 6716
ER -
TY - JOUR
AB - The ever-growing threats of multidrug-resistant bacterial infection and
chronic wound healing have created an imperative need for the development of novel
antibacterial materials and therapeutic strategies, especially for diabetic
patients infected with multidrug-resistant bacteria. In this work, the
nanocomplexes named as PB@PDA@Ag were used for eradicating multidrug-resistant
bacteria and accelerating wound healing of MRSA-infected diabetic model with the
assistance of laser irradiation. In vitro results revealed that the combinational
strategy exerted a synergistic effect for anti-MRSA through disrupting cell
integrity, producing ROS, declining ATP, and oxidizing GSH, comparing with
PB@PDA@Ag or NIR laser irradiation alone. Moreover, in vivo assay demonstrated that
this system effectively accelerated MRSA-infected diabetic wound healing by
mitigating local inflammatory response and up-regulating VEGF expression on the
wound bed. Meanwhile, satisfactory biocompatibility and negligible damage to major
organs were observed. Altogether, the aforementioned results indicate that the
combinational therapy of PB@PDA@Ag and NIR irradiation shows a great potential
application in the field of clinic infection. © 2020 Elsevier Ltd
AU - Tong, C.
AU - Zhong, X.
AU - Yang, Y.
AU - Liu, X.
AU - Zhong, G.
AU - Xiao, C.
AU - Liu, B.
AU - Wang, W.
AU - Yang, X.
C7 - 119936
DB - Scopus
KW - Diabetic wound
MRSA
PB@PDA@Ag nanocomposites
Photothermal effect
Diabetes Mellitus
Humans
Lasers
Silver
Wound Healing
Bacteria
Biocompatibility
Infrared devices
Nanosystems
antiinfective agent
dopamine derivative
ferric ferrocyanide
glutathione
polydopamine
silver nanoparticle
unclassified drug
vasculotropin
silver
Diabetic wounds
Multidrug resistants
NIR laser irradiation
Photothermal effects
Therapeutic strategy
animal experiment
animal model
animal tissue
Article
bacterial clearance
bioassay
controlled study
diabetes mellitus
female
in vitro study
mouse
multidrug resistant bacterium
nanoanalysis
nanofabrication
nonhuman
priority journal
protein expression
upregulation
wound healing
human
laser
Irradiation
M3 - Article
PY - 2020
ST - PB@PDA@Ag nanosystem for synergistically eradicating MRSA and accelerating
T2 - Biomaterials
TI - PB@PDA@Ag nanosystem for synergistically eradicating MRSA and accelerating
85081067144&doi=10.1016%2fj.biomaterials.2020.119936&partnerID=40&md5=c9ecc02eca4e4
79a20be517f9578c55f
VL - 243
ID - 5343
ER -
TY - JOUR
AB - Purpose: It was aimed to investigate the biochemical and immunohistochemical
effects of ephedrine (EPH) in bilateral ovariectomized rats. Methods: Twenty-four
Sprague Dawley female rats were divided into three groups: control group: The
abdomen was opened and closed without any treatment; ischemia-reperfusion (IR)
group: 2 h of ischemia followed by 2 h of reperfusion were allowed to cause IR
injury; IR+EPH group: oral EPH solution (5 mg/kg) was administered for 28 days.
Results: Biochemical parameters were statistically significant in group
comparisons. Increased interleukin-6 (IL-6) expression, degenerative preantral and
antral follicle cells and inflammatory cells around blood vessels were seen in IR
group. Negative IL-6 expression was observed in seminal epithelial cells, preantral
and antral follicle cells in IR+EPH group. While caspase-3 activity increased in
granulosa cells and stromal cells in IR group, caspase-3 expression was negative in
preantral and antral follicle cells in the germinal epithelium and cortex in IR+EPH
group. Conclusion: The effect of apoptosis, which occurs with the signaling that
starts in the cell nucleus, caused the cessation of the stimulating effect at the
nuclear level after EPH administration, and a decrease in the antioxidative effect
in IR damage and inflammation in the apoptotic process.
AD - Toprak, Veysel
Diyarbakir Memorial Hospital. Division of Gynecology and Obstetrics. Diyarbakir. TR
Akalin, Senem Alkan
Private Medical Practice. Division of Gynecology and Obstetrics. Diyarbakir. TR
Öcal, Ece
Private Medical Practice. Division of Perinatology. Diyarbakir. TR
Çavus, Yunus
Diyarbakir Memorial Hospital. Division of Gynecology and Obstetrics. Diyarbakir. TR
Deveci, Engin
Dicle University. Faculty of Medicine. Department of Histology and Embryology.
Diyarbakir. TR
AU - Toprak, Veysel
AU - Akalin, Senem Alkan
AU - Öcal, Ece
AU - Çavus, Yunus
AU - Deveci, Engin
C1 - 20230707
DA - 2023/00
DB - LILACS
DO - 10.1590/acb381523
KW - Antioxidants
Apoptosis
Caspase 3
Interleukin-6
LA - en
PY - 2023
SN - 0102-8650
SP - e381523-e381523
ST - Biochemical and immunohistochemical examination of the effects of ephedrine
in rat ovary tissue
T2 - Acta cir. bras
TI - Biochemical and immunohistochemical examination of the effects of ephedrine
in rat ovary tissue
86502023000100212
VL - 38
ID - 4915
ER -
TY - JOUR
AB - Introduction: Silver decorated mesoporous carbons are interesting systems
that may offer effective solutions for advanced wound care products by combining
the well-known antimicrobial activity of silver nanoparticles with the versatile
properties of ordered mesoporous carbons. Silver is being used as a topical
antimicrobial agent, especially in wound repair. However, while silver shows
bactericidal properties, it is also cytotoxic at high concentrations. Therefore,
the incorporation of silver into ordered mesoporous carbons allows to exploit both
silver’s biological effects and mesoporous carbons’ biocompatibility and
versatility with the purpose of conceiving silver-doped materials in light of the
growing health concern in wound care. Methods: The wound healing potential of an
ordered mesoporous carbon also doped with two different loadings of silver
nanoparticles (2 wt% and 10 wt%), was investigated through a biological assessment
study based on different assays (cell viability, inflammation, antibacterial tests,
macrophage-conditioned fibroblast and human keratinocyte cell cultures). Results:
The results show silver-doped ordered mesoporous carbons to positively condition
cell viability, with a cell viability percentage >70% even for 10 wt% Ag, to
modulate the expression of inflammatory cytokines and of genes involved in tissue
repair (KRT6a, VEGFA, IVN) and remodeling (MMP9, TIMP3) in different cell systems.
Furthermore, along with the biocompatibility and the bioactivity, the silver-doped
ordered mesoporous carbons still retain an antibacterial effect, as shown by a
maximum of 13.1% of inhibited area in the Halo test. The obtained results clearly
showed that the silver-doped ordered mesoporous carbons exhibit both good
biocompatibility and antibacterial effect with enhanced re-epithelialization,
angiogenesis promotion and tissue regeneration. Discussion: These findings suggest
that the exceptional properties of silver-doped ordered mesoporous carbons could be
exploited in the treatment of acute and chronic wounds and that such carbon
materials could be potential candidates for use in medical devices for wound
healing purposes, in particular, the 10 wt% loading, as the results showed to be
the most effective. © 2019 Torre et al.
AU - Torre, E.
AU - Giasafaki, D.
AU - Steriotis, T.
AU - Cassinelli, C.
AU - Morra, M.
AU - Fiorilli, S.
AU - Vitale-Brovarone, C.
AU - Charalambopoulou, G.
AU - Iviglia, G.
DB - Scopus
DO - 10.2147/IJN.S234393
KW - Metal doped carbons
Ordered mesoporous carbon
Tissue regeneration
Wound healing
Animals
Carbon
Cell Line
Cell Survival
Chronic Disease
Fibroblasts
Humans
Keratinocytes
Macrophages
Metal Nanoparticles
Mice
Re-Epithelialization
Silver
Wound Healing
carbon nanoparticle
cytokeratin 6
gelatinase B
involucrin
silver nanoparticle
vasculotropin A
antiinfective agent
carbon
metal nanoparticle
silver
acute disease
angiogenesis
animal cell
Article
biocompatibility
biological activity
cell culture
chronic wound
controlled study
epithelization
fibroblast
gene expression
keratinocyte
macrophage
nonhuman
protein expression
tissue regeneration
tissue repair
wound care
wound healing
animal
cell line
cell survival
chemistry
chronic disease
drug effect
human
mouse
physiology
M3 - Article
PY - 2019
SP - 10147-10164
ST - Silver decorated mesoporous carbons for the treatment of acute and chronic
wounds, in a tissue regeneration context
TI - Silver decorated mesoporous carbons for the treatment of acute and chronic
wounds, in a tissue regeneration context
85077707028&doi=10.2147%2fIJN.S234393&partnerID=40&md5=78d828c3ba6e6b8adb17e6edfcf6
658f
VL - 14
ID - 5421
ER -
TY - JOUR
AB - Synthetic amorphous silica is one of the most used nanomaterials, and
numerous toxicological studies have studied its effects. Most of these studies have
used an acute exposure mode to investigate the effects immediately after exposure.
However, this exposure modality does not allow the investigation of the persistence
of the effects, which is a crucial aspect of silica toxicology, as exemplified by
crystalline silica. In this paper, we extended the investigations by studying not
only the responses immediately after exposure but also after a 72 h post-exposure
recovery phase. We used a pyrolytic silica as the test nanomaterial, as this
variant of synthetic amorphous silica has been shown to induce a more persistent
inflammation in vivo than precipitated silica. To investigate macrophage responses
to pyrolytic silica, we used a combination of proteomics and targeted experiments,
which allowed us to show that most of the cellular functions that were altered
immediately after exposure to pyrolytic silica at a subtoxic dose, such as energy
metabolism and cell morphology, returned to normal at the end of the recovery
period. However, some alterations, such as the inflammatory responses and some
aldehyde detoxification proteins, were persistent. At the proteomic level, other
alterations, such as proteins implicated in the endosomal/lysosomal pathway, were
also persistent but resulted in normal function, thus suggesting cellular
adaptation.
AN - WOS:000586924800001
AU - Torres, A.
AU - Dalzon, B.
AU - Diemer, H.
AU - Fenel, D.
AU - Schoehn, G.
AU - Cianferani, S.
AU - Carriere, M.
AU - Rabilloud, T.
C7 - 1939
DA - OCT
DO - 10.3390/nano10101939
IS - 10
PY - 2020
SN - 2079-4991
ST - How Reversible Are the Effects of Fumed Silica on Macrophages? A Proteomics-
Informed View
T2 - NANOMATERIALS
TI - How Reversible Are the Effects of Fumed Silica on Macrophages? A Proteomics-
Informed View
VL - 10
ID - 6832
ER -
TY - JOUR
AB - Lung fibrosis involves the overexpression of ECM proteins, primarily
collagen, by alpha-smooth muscle actin (ASMA)-positive cells. Caveolin-1 is a
master regulator of collagen expression by cultured lung fibroblasts and of lung
fibrosis in vivo. A peptide equivalent to the caveolin-1 scaffolding domain (CSD
peptide) inhibits collagen and tenascin-C expression by normal lung fibroblasts
(NLF) and fibroblasts from the fibrotic lungs of scleroderma patients (SLF). CSD
peptide inhibits ASMA expression in SLF but not NLF. Similar inhibition of
collagen, tenascin-C, and ASMA expression was also observed when caveolin-1
expression was upregulated using adenovirus. These observations suggest that the
low caveolin-1 levels in SLF cause their overexpression of collagen, tenascin-C,
and ASMA. In mechanistic studies, MEK, ERK, JNK, and Akt were hyperactivated in
SLF, and CSD peptide inhibited their activation and altered their subcellular
localization. These studies and experiments using kinase inhibitors suggest many
differences between NLF and SLF in signaling cascades. To validate these data, we
determined that the alterations in signaling molecule activation observed in SLF
also occur in fibrotic lung tissue from scleroderma patients and in mice with
bleomycin-induced lung fibrosis. Finally, we demonstrated that systemic
administration of CSD peptide to bleomycin-treated mice blocks epithelial cell
apoptosis, inflammatory cell infiltration, and changes in tissue morphology as well
as signaling molecule activation and collagen, tenascin-C, and ASMA expression
associated with lung fibrosis. CSD peptide may be a prototype for novel treatments
for human lung fibrosis that act, in part, by inhibiting the expression of ASMA and
ECM proteins.
AN - WOS:000255501400004
AU - Tourkina, E.
AU - Richard, M.
AU - Gooz, P.
AU - Bonner, M.
AU - Pannu, J.
AU - Harley, R.
AU - Bernatchez, P. N.
AU - Sessa, W. C.
AU - Silver, R. M.
AU - Hoffman, S.
DA - MAY
DO - 10.1152/ajplung.00295.2007
IS - 5
PY - 2008
SN - 1040-0605
1522-1504
SP - L843-L861
ST - Antifibrotic properties of caveolin-1 scaffolding domain in vitro and in vivo
T2 - AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
TI - Antifibrotic properties of caveolin-1 scaffolding domain in vitro and in vivo
VL - 294
ID - 6756
ER -
TY - JOUR
AB - A St. Jude Medical Silzone was implanted in a 72-year-old female, suffering
from mitral valve disease. Four months later, the patient had acute cardiac failure
due to partial detachment of the prosthetic valve. The mitral annulus was ulcerated
and there were multiple erosions in the myocardial tissue in contact with the
prosthetic valve. Histological examination revealed chronic inflammation with
hemosiderine deposits and giant cells. No allergy to silver ions was found. The
silver-coated sewing cuff had caused a chronic inflammatory reaction due to a toxic
reaction to silver. The Silzone valve was withdrawn from the market on January
2000. Copyright © 2001 Elsevier Science B.V.
AU - Tozzi, P.
AU - Al-Darweesh, A.
AU - Vogt, P.
AU - Stumpe, F.
DB - Scopus
DO - 10.1016/S1010-7940(01)00675-3
IS - 5
KW - Endocarditis
Prosthetic heart valve
Silver toxicity
Aged
Female
Heart Valve Prosthesis
Humans
Hypersensitivity
Mitral Valve
Mitral Valve Insufficiency
Prosthesis Design
Prosthesis Failure
Silver
hemosiderin
silver nitrate
acute heart failure
aged
article
case report
female
histology
human
implantation
inflammation
mitral valve disease
priority journal
prosthesis loosening
Saint Jude heart valve prosthesis
M3 - Article
PY - 2001
SP - 729-731
ST - Silver-coated prosthetic heart valve: A double-bladed weapon
T2 - European Journal of Cardio-thoracic Surgery
TI - Silver-coated prosthetic heart valve: A double-bladed weapon
0035006437&doi=10.1016%2fS1010-7940%2801%2900675-
3&partnerID=40&md5=2114dd0b5da51f80563424c33fbea404
VL - 19
ID - 5768
ER -
TY - JOUR
AB - Since no approved therapies to restore mobility and sensation following
spinal cord injury (SCI) currently exist, a better understanding of the cellular
and molecular mechanisms following SCI that compromise regeneration or
neuroplasticity is needed to develop new strategies to promote axonal regrowth and
restore function. Physical trauma to the spinal cord results in vascular disruption
that, in turn, causes blood-spinal cord barrier rupture leading to hemorrhage and
ischemia, followed by rampant local cell death. As subsequent edema and
inflammation occur, neuronal and glial necrosis and apoptosis spread well beyond
the initial site of impact, ultimately resolving into a cavity surrounded by
glial/fibrotic scarring. The glial scar, which stabilizes the spread of secondary
injury, also acts as a chronic, physical, and chemo-entrapping barrier that
prevents axonal regeneration. Understanding the formative events in glial scarring
helps guide strategies towards the development of potential therapies to enhance
axon regeneration and functional recovery at both acute and chronic stages
following SCI. This review will also discuss the perineuronal net and how
chondroitin sulfate proteoglycans (CSPGs) deposited in both the glial scar and net
impede axonal outgrowth at the level of the growth cone. We will end the review
with a summary of current CSPG-targeting strategies that help to foster axonal
regeneration, neuroplasticity/sprouting, and functional recovery following SCI.
AN - WOS:000440979800006
AU - Tran, A. P.
AU - Warren, P. M.
AU - Silver, J.
DA - APR
DO - 10.1152/physrev.00017.2017
IS - 2
PY - 2018
SN - 0031-9333
1522-1210
SP - 881-917
ST - THE BIOLOGY OF REGENERATION FAILURE AND SUCCESS AFTER SPINAL CORD INJURY
T2 - PHYSIOLOGICAL REVIEWS
TI - THE BIOLOGY OF REGENERATION FAILURE AND SUCCESS AFTER SPINAL CORD INJURY
VL - 98
ID - 6795
ER -
TY - JOUR
AB - Using analytical chemistry techniques such as nuclear magnetic resonance
(NMR) spectroscopy and liquid or gas chromatography–mass spectrometry (LC/GC-MS),
metabolomics allows detection of most endogenous and exogenous metabolites in a
biological sample. Metabolomics has a wide range of applications, and has been
employed in nutrition science, toxicology, environmental studies, and systems
biology. Metabolomics is particularly useful in biomedical science, and has been
used for diagnostic laboratory testing, identifying targets for drug development,
and monitoring drug metabolism, mode of action, and toxicity. Despite its immense
potential, metabolomics remains underutilized in the study of spontaneous animal
diseases. Our aim was to comprehensively review the existing literature on the use
of metabolomics in spontaneous veterinary diseases. Three databases were used to
find journal articles that applied metabolomics in veterinary medicine. A screening
process was then conducted to eliminate references that did not meet the
eligibility criteria; only primary research studies investigating spontaneous
animal disease were included; 38 studies met the inclusion criteria. The main
techniques used were NMR and MS. All studies detected metabolite alterations in
diseased animals compared with non-diseased animals. Metabolomics was mainly used
to study diseases of the digestive, reproductive, and musculoskeletal systems.
Inflammatory conditions made up the largest proportion of studies when articles
were categorized by disease process. Following a comprehensive analysis of the
literature on metabolomics in spontaneous veterinary diseases, we concluded that
metabolomics, although in its early stages in veterinary research, is a promising
tool regarding diagnosis, biomarker discovery, and in uncovering new insights into
disease pathophysiology. © 2020 The Author(s).
AU - Tran, H.
AU - McConville, M.
AU - Loukopoulos, P.
DB - Scopus
DO - 10.1177/1040638720948505
IS - 5
KW - metabolomics
omics
oncology
one health
review
veterinary
Animal Diseases
Animals
Gas Chromatography-Mass Spectrometry
Magnetic Resonance Spectroscopy
Metabolomics
alanine
biological marker
carnosine
choline
creatinine
fumaric acid
gluconic acid
glutamic acid
glutamine
glycylproline
hexuronic acid
hydroxybutyric acid
isoleucine
lactone
lysine
lysophosphatidylcholine
methylamine
n acetylaspartic acid
nicotinamide phosphoribosyltransferase
palmitoleic acid
phosphorylcholine
propionic acid
pyruvic acid
serum amyloid A
silver nanoparticle
taurine
tryptophan
tyrosine
unindexed drug
animal disease
anxiety
biopsy
Bovine coronavirus
cat
cell culture
cerebrospinal fluid
citric acid cycle
cow
diabetes mellitus
diarrhea
dog
doping
drug metabolism
fever
fourier transform ion cyclotron resonance mass spectrometry
horse
isotope labeling
ketoacidosis
laboratory test
liver tissue
lymphoma
metabolite
muscular dystrophy
newborn sepsis
nonhuman
nutrition
osteoarthritis
osteochondrosis
proteomics
Review
sheep
systems biology
temperature stress
toxicology
transitional cell carcinoma
vascular disease
veterinary medicine
animal
metabolism
procedures
M3 - Review
PY - 2020
SP - 635-647
ST - Metabolomics in the study of spontaneous animal diseases
T2 - Journal of Veterinary Diagnostic Investigation
TI - Metabolomics in the study of spontaneous animal diseases
85089524445&doi=10.1177%2f1040638720948505&partnerID=40&md5=8ebe7bdb4404925c2ae490f
1be0e6d40
VL - 32
ID - 5309
ER -
TY - JOUR
AB - Psoriasis is a common T-cell-mediated immune disorder characterized by
circumscribed, red, thickened plaques with an overlying silver-white scale. It
occurs worldwide, although the incidence is lower in warmer, sunnier climates. The
primary cause of psoriasis is unknown. During an active disease state, an
underlying inflammatory mechanism is frequently involved. Many conventional
treatments focus on suppressing symptoms associated with psoriasis and have
significant side effects. This article reviews several of the researched natural
approaches to psoriasis treatment, while addressing its underlying cause.
AU - Traub, M.
AU - Marshall, K.
DB - Scopus
IS - 4
KW - Complementary Therapies
Diet Therapy
Dietary Supplements
Humans
Immunity, Cellular
Life Style
Psoriasis
Risk Factors
adalimumab
alefacept
Berberis aquifolium extract
calcipotriol
CD4 antigen
CD8 antigen
cerave
corticosteroid
curcumin
cyclosporin
dithranol
efalizumab
etanercept
flavsalve
folic acid
fumaric acid
gamma interferon
herose
infliximab
interleukin 2
leukotriene B4
leukotriene B5
methotrexate
mimyxaveeno eczema care
placebo
plant extract
prostaglandin E1
prostaglandin E2
prostaglandin E3
psoriaflora
relieva
tar
tazarotene
thromboxane A2
unclassified drug
unindexed drug
xp 828l
alternative medicine
CD4+ T lymphocyte
CD8+ T lymphocyte
clinical feature
clinical trial
dendritic cell
diet therapy
disease severity
drug indication
fatigue
folic acid deficiency
gastrointestinal symptom
headache
human
kidney injury
lifestyle modification
liver fibrosis
liver toxicity
megaloblastic anemia
nausea
pathophysiology
phototherapy
prevalence
psoriasis
PUVA
rebound
review
risk factor
side effect
sun exposure
symptom
Th1 cell
treatment indication
tropic climate
M3 - Review
PY - 2007
SP - 319-330
ST - Psoriasis - Pathophysiology, conventional, and alternative approaches to
treatment
T2 - Alternative Medicine Review
TI - Psoriasis - Pathophysiology, conventional, and alternative approaches to
treatment
37549028062&partnerID=40&md5=2adb4821745139c33fbd22bf00f9d334
VL - 12
ID - 5834
ER -
TY - JOUR
AB - The molecular responses of macrophages to copper-based nanoparticles have
been investigated via a combination of proteomic and biochemical approaches, using
the RAW264.7 cell line as a model. Both metallic copper and copper oxide
nanoparticles have been tested, with copper ion and zirconium oxide nanoparticles
used as controls. Proteomic analysis highlighted changes in proteins implicated in
oxidative stress responses (superoxide dismutases and peroxiredoxins), glutathione
biosynthesis, the actomyosin cytoskeleton, and mitochondrial proteins (especially
oxidative phosphorylation complex subunits). Validation studies employing
functional analyses showed that the increases in glutathione biosynthesis and in
mitochondrial complexes observed in the proteomic screen were critical to cell
survival upon stress with copper-based nanoparticles; pharmacological inhibition of
these two pathways enhanced cell vulnerability to copper-based nanoparticles, but
not to copper ions. Furthermore, functional analyses using primary macrophages
derived from bone marrow showed a decrease in reduced glutathione levels, a
decrease in the mitochondrial transmembrane potential, and inhibition of
phagocytosis and of lipopolysaccharide-induced nitric oxide production. However,
only a fraction of these effects could be obtained with copper ions. In conclusion,
this study showed that macrophage functions are significantly altered by copper-
based nanoparticles. Also highlighted are the cellular pathways modulated by cells
for survival and the exemplified cross-toxicities that can occur between copper-
based nanoparticles and pharmacological agents. Molecular & Cellular Proteomics 12:
10.1074/mcp.M113.030742, 3108-3122, 2013.
AN - WOS:000328816000008
AU - Triboulet, S.
AU - Carriere, M.
AU - Diemer, H.
AU - Proamer, F.
AU - Habert, A.
AU - Chevallet, M.
AU - Strub, J. M.
AU - Hanau, D.
AU - Van Dorsselaer, A.
AU - Rabilloud, T.
DA - NOV
DO - 10.1074/mcp.M113.030742
IS - 11
PY - 2013
SN - 1535-9476
1535-9484
SP - 3108-3122
ST - Molecular Responses of Mouse Macrophages to Copper and Copper Oxide
Nanoparticles Inferred from Proteomic Analyses
T2 - MOLECULAR & CELLULAR PROTEOMICS
TI - Molecular Responses of Mouse Macrophages to Copper and Copper Oxide
Nanoparticles Inferred from Proteomic Analyses
VL - 12
ID - 6828
ER -
TY - JOUR
AB - The purpose of the current studies was to determine if systemic exposure of
various metallic nanoparticles differing in size and composition [silver (Ag-NPs,
25, 40 and 80 nm), copper-oxide (Cu-NPs, 40 and 60 nm) or gold (Au-NPs, 3 and 5
nm)] can induce the release of pro-inflammatory mediators that influence the
restrictive nature of the blood-brain barrier (BBB) in vitro. Confluent porcine
brain microvessel endothelial cells (pBMECs) (8-12 days) were treated with various
metallic nanoparticles (15μg/ml). Extracellular concentrations of pro-inflammatory
mediators (IL-1β, TNFα and PGE2) were evaluated using ELISA. pBMECs were cultured
in standard 12-well Transwell® inserts, and permeability was evaluated by measuring
the transport of fluorescein across the pBMEC monolayers. PGE2 release following
Cu-NP exposure was significantly increased when compared to the control. Similar
results were observed for Ag-NPs but not Au-NPs. The secretion of TNFα and IL-1β
was observed for both Cu-NPs and Ag-NPs but not in response to Au-NPs. The post-
treatment time profiles of TNFα and IL-1β revealed that the IL-1β response was more
persistent. The permeability ratios (exposure/control) were significantly greater
following exposure to Cu-NPs or Ag-NPs, compared to Au-NPs. Together, these data
suggest that the composition and size of NPs can cause significant pro-inflammatory
response that can influence the integrity of the BBB. © 2014 Informa Healthcare
USA, Inc. All rights reserved: reproduction in whole or part not permitted.
AU - Trickler, W. J.
AU - Lantz-Mcpeak, S. M.
AU - Robinson, B. L.
AU - Paule, M. G.
AU - Slikker Jr, W.
AU - Biris, A. S.
AU - Hussain, S. M.
AU - Kanungo, J.
AU - Gonzalez, C.
AU - Ali, S. F.
DB - Scopus
DO - 10.3109/03602532.2013.873450
IS - 2
KW - Blood-brain barrier
Lipopolysaccharide (LPS)
Metallic-colloidal nanoparticles
Neuroinflamation
Neurotoxicity
Porcine brain microvessel endothelial cells
Animals
Blood-Brain Barrier
Dinoprostone
Endothelial Cells
Interleukin-1beta
Metal Nanoparticles
Microvessels
Particle Size
Surface Properties
Swine
copper nanoparticle
fluorescein
gold nanoparticle
interleukin 1beta
prostaglandin E2
silver nanoparticle
autacoid
metal nanoparticle
animal cell
blood brain barrier
brain capillary endothelial cell
controlled study
cytokine release
experimental pig
in vitro study
nonhuman
particle size
review
animal
chemistry
cytology
drug effects
endothelium cell
immunology
microvasculature
secretion (process)
surface property
swine
toxicity
M3 - Review
PY - 2014
SP - 224-231
ST - Porcine brain microvessel endothelial cells show pro-inflammatory response to
the size and composition of metallic nanoparticles
T2 - Drug Metabolism Reviews
TI - Porcine brain microvessel endothelial cells show pro-inflammatory response to
the size and composition of metallic nanoparticles
84899413985&doi=10.3109%2f03602532.2013.873450&partnerID=40&md5=bb4de8453a43f2fe6ab
8a9690c3035da
VL - 46
ID - 5602
ER -
TY - JOUR
AB - To reduce the incidence of implant-associated infection, we previously
developed a novel coating technology using hydroxyapatite (HA) containing silver
(Ag). This study examined in vivo acute and subacute toxicity associated with the
Ag-HA coating in rat tibiae. Ten-week-old rats received implantation of HA-, 2% Ag-
HA-, or 50% Ag-HA-coated titanium rods. Concentrations of silver in serum, brain,
liver, kidneys, and spleen were measured in the acute phase (2-4 days after
treatment) and subacute phase (4-12 weeks after treatment). Biochemical and
histological examinations of those organs were also performed. Mean serum silver
concentration peaked in the acute phase and then gradually decreased. Mean silver
concentrations in all examined organs from the 2% Ag-HA coating groups showed no
significant differences compared with the HA coating group. No significant
differences in mean levels of glutamic-oxaloacetic transaminase, glutamic-pyruvic
transaminase, lactate dehydrogenase, creatinine, or blood urea nitrogen were seen
between the three groups and controls. Histological examinations of all organs
revealed no abnormal pathologic findings. No acute or subacute toxicity was seen in
vivo for 2% Ag-HA coating or HA coating. Ag-HA coatings on implants may represent
biologically safe antibacterial biomaterials and may be of value for reducing
surgical-site infections related to implantation. © 2014 Masatsugu Tsukamoto et al.
AU - Tsukamoto, M.
AU - Miyamoto, H.
AU - Ando, Y.
AU - Noda, I.
AU - Eto, S.
AU - Akiyama, T.
AU - Yonekura, Y.
AU - Sonohata, M.
AU - Mawatari, M.
C7 - 902343
DB - Scopus
DO - 10.1155/2014/902343
KW - Animals
Durapatite
Rats
Silver
Tibia
Titanium
creatinine
hydroxyapatite
nitrogen
silver
titanium
urea
antiinfective agent
biomaterial
animal experiment
animal tissue
article
body weight
brain
controlled study
foreign body granuloma
histopathology
in vivo study
kidney
liver
male
nonhuman
orthopedic implant
prosthesis infection
rat
spleen
tibia
animal
pathology
surgery
M3 - Article
PY - 2014
ST - Acute and subacute toxicity in vivo of thermal-sprayed silver containing
hydroxyapatite coating in rat tibia
TI - Acute and subacute toxicity in vivo of thermal-sprayed silver containing
hydroxyapatite coating in rat tibia
84897497385&doi=10.1155%2f2014%2f902343&partnerID=40&md5=319fc3b10f34a4cdc4c8dfd914
60d6c1
VL - 2014
ID - 5567
ER -
TY - JOUR
AB - Nowadays, the unique features of nanoparticles (NPs) have encouraged new
applications in different areas including biology, medicine, agriculture, and
electronics. Their quick joining into daily life not only enhances the uses of NPs
in a wide range of modern technologies but also their release into the aquatic
environment causes inevitable environmental concerns. On the other hand boron
exhibits key physiological effects on biological systems. This research was
designed for evaluating the toxicity of magnetite nanoparticles (Fe3O4-MNPs) on
aquatic organisms and obtaining data for the information gap in this area. In this
study, Rainbow trout (Oncorhynchus mykiss) was considered as an aquatic indicator,
and trials were designed as Ulexite (a boron mineral, UX) treatment against
exposure to Fe3O4-MNPs. Synthesized and characterized Fe3O4-MNPs were exposed to
rainbow trouts in wide spectrum concentrations (0.005-0.08 mL/L) to analyze its
lethal dose (LC50) and cytoprotective properties by UX treatment were assessed
against Fe3O4-MNPs applications for 96 h. For the initial toxicity analysis,
hematological parameters (blood cell counts) were examined in experimental groups
and micronucleus (MN) assay was performed to monitor nuclear abnormalities after
exposure to NPs. Biochemical analyzes in both blood and liver samples were utilized
to assess antioxidant/oxidative stress and inflammatory parameters. Also, 8-
hydroxy-2'-deoxyguanosine (8-OHdG) assay was used to investigate oxidative DNA
lesions and Caspase-3 analysis was performed on both blood and liver tissues to
monitor apoptotic cell death occurrence. When antioxidant enzymes in blood and
liver tissue were examined, time-dependent decreases in activity were determined in
SOD, CAT, GPx, and GSH enzymes, while increased levels of MDA and MPO parameters
were observed in respect to Fe3O4-MNPs exposure. It was found that TNF-alpha, Il-6
levels were enhanced against Fe3O4-MNPs treatment, but Nrf-2 levels were decreased
at the 46th and 96th h. In the 96th application results, all parameters were
statistically significant (p < 0.05) in blood and liver tissue, except for the IL-6
results. It was determined that the frequency of MN, the level of 8-OHdG and
caspase-3 activity increased in respect to Fe3O4-MNPs exposure over time. Treatment
with UX alleviated Fe3O4-MNPs-induced hematotoxic and hepatotoxic alterations as
well as oxidative and genetic damages. Our findings offer strong evidence for the
use of UX as promising, safe and natural protective agents against environmental
toxicity of magnetite nanoparticles.
AN - WOS:000908273100001
AU - Ucar, A.
AU - Arslan, M. E.
AU - Yeltekin, A. C.
AU - Ozgeris, F. B.
AU - Yildirim, O. C.
AU - Parlak, V.
AU - Alak, G.
AU - Turkez, H.
AU - Atamanalp, M.
C6 - JAN 2023
DA - 2023 JAN 5
DO - 10.1080/01480545.2022.2164298
PY - 2023
SN - 0148-0545
1525-6014
ST - Neutralization of iron oxide magnetic nanoparticle aquatoxicity on
Oncorhynchus mykiss via supplementation with ulexite
T2 - DRUG AND CHEMICAL TOXICOLOGY
TI - Neutralization of iron oxide magnetic nanoparticle aquatoxicity on
Oncorhynchus mykiss via supplementation with ulexite
ID - 6679
ER -
TY - JOUR
AB - The prevalent exposition of metallic nanoparticles (MNPs) to the aquatic
medium and their negative influence on human life is one of the major concerns
global. Stress mechanization, as a non-specific and pervasive response, involves
all physiological systems, particularly the closely interconnected neuroendocrine
and immune systems. In this study, which was designed to obtain more data on the
biological effects of ulexit, which prevents oxidative DNA damage by protecting
against toxicity damage and offers new antioxidant roles. The concomitant use of
ulexite (UX, as 18.75 mg/l) as a natural therapeutic agent against exposure to
magnetic nanoparticles (Fe3O4-MNPs/0.013 ml/l) on Oncorhynchus mykiss was
investigated for 96 h. The brain tissues were taken at the 48th and 96th hours of
the trial period, the effects on neurotoxic, pro-inflammatory cytokine genes,
antioxidant immune system, DNA and apoptosis mechanisms were analyzed. In the
present study, it was determined that AChE activity and BDNF level in the brain
tissue decreased over time in the Fe3O4-MNPs group compared to the control, and UX
tried to depress this inhibition. While inhibition was determined in antioxidant
system biomarkers (SOD, CAT, GPx, and GSH values), an induction was observed in
lipid peroxidation indicators (MDA and MPO values) in Fe3O4-MNPs applied group. The
same group data showed that TNF-alpha, IL-6, 8-OHdG and caspase-3 levels were
increased, but Nrf-2 levels were decreased. The alterations in all biomarkers were
found to be significant at the p < 0.05 level. In general, it was determined that
Fe3O4-MNPs caused stress in O. mykiss and UX exhibited a positive effect on this
stress management.
AN - WOS:000806604000003
AU - Ucar, A.
AU - Parlak, V.
AU - Ozgeris, F. B.
AU - Yeltekin, A. C.
AU - Arslan, M. E.
AU - Alak, G.
AU - Turkez, H.
AU - Kocaman, E. M.
AU - Atamanalp, M.
C6 - MAY 2022
C7 - 155718
DA - SEP 10
DO - 10.1016/j.scitotenv.2022.155718
PY - 2022
SN - 0048-9697
1879-1026
ST - Magnetic nanoparticles-induced neurotoxicity and oxidative stress in brain of
rainbow trout: Mitigation by ulexite through modulation of antioxidant, anti-
inflammatory, and antiapoptotic activities
T2 - SCIENCE OF THE TOTAL ENVIRONMENT
TI - Magnetic nanoparticles-induced neurotoxicity and oxidative stress in brain of
rainbow trout: Mitigation by ulexite through modulation of antioxidant, anti-
inflammatory, and antiapoptotic activities
VL - 838
ID - 6349
ER -
TY - JOUR
AB - The potential for ingestion of copper oxide nanomaterials (CuO NMs) is
increasing due to their increased exploitation. Investigation of changes in gene
expression allows toxicity to be detected at an early stage of NM exposure and can
enable investigation of the mechanism of toxicity. Here, undifferentiated Caco-2
cells, differentiated Caco-2 cells, Caco-2/HT29-MTX (mucus secreting) and
Caco-2/Raji B (M cell model) co-cultures were exposed to CuO NMs and copper
sulphate (CuSO4) in order to determine their impacts. Cellular responses were
measured in terms of production of reactive oxygen species (ROS), the gene
expression of an antioxidant (haem oxygenase 1 (HMOX1)), the pro-inflammatory
cytokine (interleukin 8 (IL8)), the metal binding (metallothionein 1A and 2A (MT1A
and MT2A)) and the mucus secreting (mucin 2 (MUC2)), as well as HMOX-1 protein
level. While CuSO4 induced ROS production in cells, no such effect was observed for
CuO NMs. However, these particles did induce an increase in the level of HMOX-1
protein and upregulation of HMOX1, MT2A, IL8 and MUC2 genes in all cell models. In
conclusion, the expression of HMOX1, IL8 and MT2A were responsive to CuO NMs at 4
to 12 h post exposure when investigating the toxicity of NMs using intestinal in
vitro models. These findings can inform the selection of endpoints, timepoints and
models when investigating NM toxicity to the intestine in vitro in the future.
AN - WOS:000646273700006
AU - Ude, V. C.
AU - Brown, D. M.
AU - Stone, V.
AU - Leonard, M.
C6 - APR 2021
C7 - 105161
DA - AUG
DO - 10.1016/j.tiv.2021.105161
PY - 2021
SN - 0887-2333
1879-3177
ST - Time dependent impact of copper oxide nanomaterials on the expression of
genes associated with oxidative stress, metal binding, inflammation and mucus
secretion in single and co-culture intestinal in vitro models
TI - Time dependent impact of copper oxide nanomaterials on the expression of
genes associated with oxidative stress, metal binding, inflammation and mucus
secretion in single and co-culture intestinal in vitro models
VL - 74
ID - 6686
ER -
TY - JOUR
AB - Zinc oxide (ZnO) NPs, owing to their broad biomedical applications, have
recently attracted the scientific community with incredible interest as therapeutic
agents. So, the present study aims at preparation of ZnO NPs, using Tragia
involucrata leaf extract and exploring their capability as antioxidant, anticancer
and anti-inflammatory agents. Besides, the ointment formulation and electrochemical
studies were also carried out in this work. The antioxidant activity of the
synthesized ZnO NPs was evaluated using DPPH assay method and the results clearly
showed higher inhibition of about 70% and lower inhibition of about 14% for 100 mu
g/ml and 25 mu g/ml concentrations, respectively. The cytotoxic effects of ZnO NPs
were evaluated against human cancer cell lines such as A549 (lungs), HeLa
(cervical), HeP-2 (laryngeal) and MCF-7 (breast). The outcome of this investigation
confirmed the effectiveness of the synthesized NPs against HeP-2 even at the lowest
concentration. The anti-inflammatory activity was measured by the inhibition of
protein denaturation assay. A higher inhibition of about 54% was noticed at the
concentration of 100 mu g/ml. In the case of the ointment formulation study, the
pastes prepared using the biosynthesized ZnO NPs and commercially available ZnO
powder were compared and evaluated using the parameters such as pH, spreadability,
moisture content, extrudability, foamability and physical examinations. As it has
been noticed that all the observed parameters were matching well with those of the
commercially available ZnO powder, ZnO NPs, synthesized using Tragia involucrata,
may be suggested for the clinical trials. Cyclic voltammetry was used to measure
the specific capacitance of the synthesized ZnO NPs for different scan rates. The
results of this study showed the gradual decrease in specific capacitance value for
the corresponding increase in scan rates. Therefore, the results of present study
indicated that ZnO NPs prepared using Tragia involucrata leaves were found to be
effective for all the above chosen applications and hence, have multifunctional
capacity.
AN - WOS:000773835600002
AU - Udhayan, S.
AU - Udayakumar, R.
AU - Gurusamy, K.
AU - Kalaichelvan, V. K.
AU - Gopalasatheeskumar, K.
C6 - MAR 2022
DA - JUN
DO - 10.1007/s12010-022-03866-z
IS - 6
PY - 2023
SN - 0273-2289
1559-0291
SP - 3764-3786
ST - Tragia involucrata Leaf-Mediated ZnO NPs: Biomedical Applications, Ointment
Formulation and Electrochemical Studies
T2 - APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
TI - Tragia involucrata Leaf-Mediated ZnO NPs: Biomedical Applications, Ointment
Formulation and Electrochemical Studies
VL - 195
ID - 6700
ER -
TY - JOUR
AB - Diabetes wounds take longer to heal due to extended inflammation, decreased
angiogenesis, bacterial infection, and oxidative stress. These factors underscore
the need for biocompatible and multifunctional dressings with appropriate
physicochemical and swelling properties to accelerate wound healing. Herein,
insulin (Ins)-loaded, and silver (Ag) coated mesoporous polydopamine (mPD)
nanoparticles were synthesized (Ag@Ins-mPD). The nanoparticles were dispersed into
polycaprolactone/methacrylated hyaluronate aldehyde dispersion, electrospun to form
nanofibers, and then photochemically crosslinked to form a fibrous hydrogel. The
nanoparticle, fibrous hydrogel, and nanoparticle-reinforced fibrous hydrogel were
characterized for their morphological, mechanical, physicochemical, swelling, drug-
release, antibacterial, antioxidant, and cytocompatibility properties. The diabetic
wound reconstruction potential of nanoparticle-reinforced fibrous hydrogel was
studied using BALB/c mice. The results indicated that Ins-mPD acted as a reductant
to synthesize Ag nanoparticles on their surface, held antibacterial and antioxidant
potential, and their mesoporous properties are crucial for insulin loading and
sustained release. The nanoparticle-reinforced scaffolds were uniform in
architecture, porous, mechanically stable, showed good swelling, and possessed
superior antibacterial, and cell-responsive properties. Furthermore, the designed
fibrous hydrogel scaffold demonstrated good angiogenic, anti-inflammatory,
increased collagen deposition, and faster wound repair capabilities, therefore, it
could be used as a potential candidate for diabetic wound treatment. © 2023
Elsevier B.V.
AU - Ullah, S.
AU - Hussain, Z.
AU - Ullah, I.
AU - Wang, L.
AU - Mehmood, S.
AU - Liu, Y.
AU - Mansoorianfar, M.
AU - Liu, X.
AU - Ma, F.
AU - Pei, R.
C7 - 125738
DB - Scopus
DO - 10.1016/j.ijbiomac.2023.125738
KW - Diabetic wound healing
Fibrous hydrogel
Hyaluronic acid
Insulin
Mesoporous nanoparticles
Polydopamine
Antioxidants
Biocompatibility
Glucose
Hydrogels
Mammals
Mesoporous materials
Reinforcement
collagen
hyaluronic acid
hydrogel
insulin
mesoporous polydopamine nanoparticle
nanoparticle
polycaprolactone
silver nanoparticle
unclassified drug
Antibacterials
Diabetic wounds
Hyaluronate
Mesoporous
Mesoporous nanoparticle
Property
Wound healing
animal experiment
animal model
animal tissue
Article
cross linking
diabetic wound
dispersion
drug synthesis
electrospinning
freeze drying
male
molecular weight
mouse
nonhuman
physical chemistry
slow drug release
wound healing
M3 - Article
PY - 2023
ST - Mussel bioinspired, silver-coated and insulin-loaded mesoporous polydopamine
nanoparticles reinforced hyaluronate-based fibrous hydrogel for potential diabetic
wound healing
TI - Mussel bioinspired, silver-coated and insulin-loaded mesoporous polydopamine
nanoparticles reinforced hyaluronate-based fibrous hydrogel for potential diabetic
wound healing
85165947835&doi=10.1016%2fj.ijbiomac.2023.125738&partnerID=40&md5=36124586917f58b1b
cf7b26e174225a9
VL - 247
ID - 5018
ER -
TY - JOUR
AB - Neoplastic cells have co-opted inflammatory receptors and signaling molecules
that potentiate inflammation. Activated inflammatory pathways lead to neo-
angiogenesis, lymph-angiogenesis, immunosuppression, tumor growth, proliferation
and metastasis. This cancer-sustaining inflammation is a critical target to arrest
cancer growth. Multiple drug resistance, high cost, low oral bioavailability and
serious side effects have rendered conventional cytotoxic chemotherapeutics less
impressive. The aim of this research was to achieve cancer debulking and
proliferation prevention by limiting ‘cancer-sustaining’ tumor niche inflammation
through non-conventional oral approach employing anti-inflammatory agents and
avoiding conventional cytotoxic agents. Synergistic anti-inflammatory agents, i.e.
celecoxib as selective COX-2 inhibitor and montelukast as cysteinyl leukotriene
receptor antagonist, were selected. Silver nanoparticles (AgNPs) were used as
nanocarriers because of their efficient synergistic anti-neoplastic effects and
excellent oral drug delivery potential. Specifically, selected drugs were co-
conjugated onto AgNPs. Synthesized nanoparticles were then surface-modified with
poly (vinyl alcohol) to control particle size, avoid opsonization/preferred
cellular uptake and improve dispersion. Surface plasmon resonance analysis,
particle size analysis, DSC, TGA, XRD, FTIR and LIBS analysis confirmed the
successful conjugation of drugs and efficient polymer coating with high loading
efficiency. In-vitro, the nanoparticles manifested best and sustained release in
moderately acidic (pH 4.5) milieu enabling passive tumor targeting potential. In-
vivo, synthesized nanoparticles exhibited efficient dose-dependent anti-
inflammatory activity reducing the dose up to 25-fold. The formulation also
manifested hemo-compatibility, potent anti-denaturation activity and dose-dependent
in-vitro and in-vivo anti-cancer potential against MCF-7 breast cancer and Hep-G2
liver cancer cell lines in both orthotopic and subcutaneous xenograft cancer
models. The anti-inflammatory nanoparticles manifested tumor specific release
potential exhibiting selective cytotoxicity at cancerous milieu with slightly
acidic environment and activated inflammatory pathways. The formulation displayed
impressive oral bioavailability, sustained release, negligible cytotoxicity against
THLE-2 normal human hepatocytes, low toxicity (high LD50) and wide therapeutic
window. Results suggest promise of developed nanomaterials as hemo-compatible,
potent, cheaper, less-toxic oral anti-inflammatory and non-conventional anti-cancer
agents. © 2022, Iran University of Science and Technology. All rights reserved.
AU - Ur Rehman, F.
AU - Naz, S. S.
AU - Dar, M. J.
AU - Malik, A.
AU - Qindeel, M.
AU - Baino, F.
AU - Wahid, F.
AU - Rahdar, A.
AU - Munir, S.
AU - Qaisar, S.
AU - Shah, K. U.
AU - Razlansari, M.
DB - Scopus
IS - 2
KW - Anti-cancer
Anti-inflammatory
Drug delivery
Nanoparticles
Non-conventional chemotherapy
M3 - Article
PY - 2022
ST - Multifunctional Silver-based Nanomaterials for Non-conventional Oral Cancer
Therapy through Simultaneous LOX and Selective COX-2 inhibition
T2 - Iranian Journal of Materials Science and Engineering
TI - Multifunctional Silver-based Nanomaterials for Non-conventional Oral Cancer
Therapy through Simultaneous LOX and Selective COX-2 inhibition
85142651332&partnerID=40&md5=e3ffaf2282d0af703083c9ba0e6ce758
VL - 19
ID - 5083
ER -
TY - JOUR
AB - Rambutan (Nephelium lappaceum) is a traditional fruit originally from Asia
and now widely available in Central America. The rind of rambutan is rich in
antioxidants, especially polyphenols, which have natural anti-inflammatory and
antimicrobial properties. Rambutan polyphenolic extract (RPE) was encapsulated in
multi-component electrospun nanofiber (ESNF) templates. These nanofibers were
decorated with silver nanoparticles (AgNPs) via in situ electrochemical reduction.
The RPE-loaded and AgNPs-decorated ESNF (AgNPs/RPE-ESNF) were evaluated for their
hydrophilic, antioxidant, and antimicrobial properties. Results indicate that the
addition of RPE to synthetic polycaprolactone (PCL) ESNF improves hydrophilicity
and cell attachment and proliferation in vitro. In addition, a synergistic
antibacterial and cell proliferation effect between AgNPs and RPE was observed when
they were combined in the multi-functionalized ESNF system. The effectiveness of
AgNPs/RPE-PCL ESNF as an antioxidant and antibacterial matrix could potentially
promote wound healing and also serve as a hybrid scaffold for tissue engineering.
AN - WOS:000541718400006
AU - Urena-Saborio, H.
AU - Rodriguez, G.
AU - Madrigal-Carballo, S.
AU - Gunasekaran, S.
C7 - 100687
DA - JUN
DO - 10.1016/j.mtla.2020.100687
PY - 2020
SN - 2589-1529
ST - Characterization and applications of silver nanoparticles-decorated
electrospun nanofibers loaded with polyphenolic extract from rambutan (Nepelium
lappaceum)
T2 - MATERIALIA
TI - Characterization and applications of silver nanoparticles-decorated
electrospun nanofibers loaded with polyphenolic extract from rambutan (Nepelium
lappaceum)
VL - 11
ID - 6116
ER -
TY - JOUR
AB - The respiratory tract is one of the most accessible ones to exogenous
nanoparticles, yet drug delivery by their means to it is made extraordinarily
challenging because of the plexus of aerodynamic, hemodynamic and biomolecular
factors at cellular and extracellular levels that synergistically define the safety
and efficacy of this process. Here, the use of inorganic nanoparticles (INPs) for
inhalable diagnostics and therapies of the lung is viewed through the prism of the
history of studies on the interaction of INPs with the lower respiratory tract. The
most conceptually and methodologically innovative and illuminative studies are
referred to in the chronological order, as they were reported in the literature,
and the trends in the progress of understanding this interaction of immense
therapeutic and toxicological significance are being deduced from it. The most
outstanding actual trends delineated include the diminishment of toxicity via
surface functionalization, cell targeting, tagging and tracking via controlled
binding and uptake, hybrid INP treatments, magnetic guidance, combined drug and
gene delivery, use as adjuvants in inhalable vaccines, and other. Many of the
understudied research directions, which have been accomplished by the
nanostructured organic polymers in the pulmonary niche, are discussed. The progress
in the use of INPs as inhalable diagnostics or therapeutics has been hampered by
their well-recognized inflammatory potential and toxicity in the respiratory tract.
However, the annual numbers of methodologically innovative studies have been on the
rise throughout the past two decades, suggesting that this is a prolific direction
of research, its comparatively poor commercial takings notwithstanding. Still, the
lack of consensus on the effects of many INP compositions at low but
therapeutically effective doses, the plethora of contradictory reports on
ostensibly identical chemical compositions and NP properties, and the many cases of
antagonism in combinatorial NP treatments imply that the rational design of
inhalable medical devices based on INPs must rely on qualitative principles for the
most part and embrace a partially stochastic approach as well. At the same time,
the fact that the most studied INPs for pulmonary applications have been those with
some of the thickest records of pulmonary toxicity, e.g., carbon, silver, gold,
silica and iron oxide, is a silent call for the expansion of the search for new
inorganic compositions for use in inhalable therapies to new territories. © 2023
The Author(s)
AU - Uskoković, V.
C7 - 102903
DB - Scopus
DO - 10.1016/j.cis.2023.102903
KW - Aerosol
Inhaler
Lung
Nanoparticle
Pulmonary
Respiratory
Nanoparticles
Nanostructures
Polymers
Biological organs
Diagnosis
Gene transfer
Iron oxides
Organic polymers
Silica
Stochastic systems
drug
nanomaterial
nanoparticle
polymer
Bio-molecular
Cellular levels
Haemodynamics
Inhalable
Respiratory tract
chemistry
Toxicity
M3 - Review
PY - 2023
ST - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
T2 - Advances in Colloid and Interface Science
TI - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
85152625854&doi=10.1016%2fj.cis.2023.102903&partnerID=40&md5=dfdb3db920aec633661e39
b131e190cb
VL - 315
ID - 4980
ER -
TY - JOUR
AB - The respiratory tract is one of the most accessible ones to exogenous
nanoparticles, yet drug delivery by their means to it is made extraordinarily
challenging because of the plexus of aerodynamic, hemodynamic and biomolecular
factors at cellular and extracellular levels that synergistically define the safety
and efficacy of this process. Here, the use of inorganic nanoparticles (INPs) for
inhalable diagnostics and therapies of the lung is viewed through the prism of the
history of studies on the interaction of INPs with the lower respiratory tract. The
most conceptually and methodologically innovative and illuminative studies are
referred to in the chronological order, as they were reported in the literature,
and the trends in the progress of understanding this interaction of immense
therapeutic and toxicological significance are being deduced from it. The most
outstanding actual trends delineated include the diminishment of toxicity via
surface functionalization, cell targeting, tagging and tracking via controlled
binding and uptake, hybrid INP treatments, magnetic guidance, combined drug and
gene delivery, use as adjuvants in inhalable vaccines, and other. Many of the
understudied research directions, which have been accomplished by the
nanostructured organic polymers in the pulmonary niche, are discussed. The progress
in the use of INPs as inhalable diagnostics or therapeutics has been hampered by
their well-recognized inflammatory potential and toxicity in the respiratory tract.
However, the annual numbers of methodologically innovative studies have been on the
rise throughout the past two decades, suggesting that this is a prolific direction
of research, its comparatively poor commercial takings notwithstanding. Still, the
lack of consensus on the effects of many INP compositions at low but
therapeutically effective doses, the plethora of contradictory reports on
ostensibly identical chemical compositions and NP properties, and the many cases of
antagonism in combinatorial NP treatments imply that the rational design of
inhalable medical devices based on INPs must rely on qualitative principles for the
most part and embrace a partially stochastic approach as well. At the same time,
the fact that the most studied INPs for pulmonary applications have been those with
some of the thickest records of pulmonary toxicity, e.g., carbon, silver, gold,
silica and iron oxide, is a silent call for the expansion of the search for new
inorganic compositions for use in inhalable therapies to new territories.
AN - WOS:000984844700001
AU - Uskokovic, V.
C6 - APR 2023
C7 - 102903
DA - MAY
DO - 10.1016/j.cis.2023.102903
PY - 2023
SN - 0001-8686
1873-3727
ST - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
T2 - ADVANCES IN COLLOID AND INTERFACE SCIENCE
TI - Lessons from the history of inorganic nanoparticles for inhalable diagnostics
and therapeutics
VL - 315
ID - 6801
ER -
TY - JOUR
AB - Background: Silver nanoparticles (AgNP) have gained much attention in recent
years due to their biomedical applications, especially as antimicrobial agents.
AgNP may be used in poultry production as an alternative to the use of antibiotic
growth promoter. However, little is known about the impact of oral administration
of AgNP on the gut microbiota and the immune system. The aim of the present study
was to investigate the effects of AgNP on growth, hematological and immunological
profile as well as intestinal microbial composition in broilers challenged with
Campylobacter jejuni (C.jejuni). Results: AgNP did not affect the intestinal
microbial profile of birds. The body weight gain and the relative weights of bursa
and spleen were reduced when supplemented with AgNP. There was no difference with
respect to packed cell volume. However, the plasma concentrations of IgG and IgM
were lower in birds receiving AgNP compared to the non-supplemented control group.
The expression of TNF-alpha and NF-kB at mRNA level was significantly higher in
birds receiving AgNP. Conclusions: The application of AgNP via the drinking water
in the concentration of 50 ppm reduced broiler growth, impaired immune functions
and had no antibacterial effect on different intestinal bacterial groups, which may
limit the applicability of AgNP against C. jejuni in broiler chickens.
AN - WOS:000419016800001
AU - Vadalasetty, K. P.
AU - Lauridsen, C.
AU - Engberg, R. M.
AU - Vadalasetty, R.
AU - Kutwin, M.
AU - Chwalibog, A.
AU - Sawosz, E.
C7 - 1
DA - JAN 2
DO - 10.1186/s12917-017-1323-x
PY - 2018
SN - 1746-6148
ST - Influence of silver nanoparticles on growth and health of broiler chickens
after infection with Campylobacter jejuni
T2 - BMC VETERINARY RESEARCH
TI - Influence of silver nanoparticles on growth and health of broiler chickens
after infection with Campylobacter jejuni
VL - 14
ID - 6056
ER -
TY - JOUR
AB - Nanotechnology is a field that is burgeoning day by day, making an impact in
all spheres of human life. Biological methods of synthesis have paved way for the
"greener synthesis" of nanoparticles and these have proven to be better methods due
to slower kinetics, they offer better manipulation and control over crystal growth
and their stabilization. This has motivated an upsurge in research on the synthesis
routes that allow better control of shape and size for various nanotechnological
applications. Nanosilver has developed as a potent antibacterial, antifungal, anti-
viral and anti-inflammatory agent. The recent advancement in the field includes the
enzymatic method of synthesis suggesting enzymes to be responsible for the
nanoparticle formation. The biomedical applications of silver nanoparticle can be
effective by the use of biologically synthesized nanoparticles which minimize the
factors such as toxicity and cost and are found to be exceptionally stable. The
targeting of cancer cells using silver nanoparticles has proven to be effective,
but neither the exact mechanism of action nor the modes of activation of the
downstream signaling molecules have been revealed yet. The review illustrates a
probable signaling pathway and mechanism by which silver nanoparticles target the
cancer cells. The current review also examines the historical background of
nanoparticles, role of silver nanoparticles in various biomedical applications and
also focusing on better methods of the synthesis of nanoparticles. © 2009 Elsevier
Inc. All rights reserved.
AU - Vaidyanathan, R.
AU - Kalishwaralal, K.
AU - Gopalram, S.
AU - Gurunathan, S.
DB - Scopus
DO - 10.1016/j.biotechadv.2009.08.001
IS - 6
KW - Anti-bacterial
Anti-inflammatory agent
Anti-viral
Antifungal
Cancer therapy
Green synthesis
Nanotechnology
Silver
Bacteriology
Cells
Grain boundaries
Growth kinetics
Nanoparticles
Signaling
antiinfective agent
silver
biological method
cancer
enzyme activity
nanotechnology
stabilization
review
M3 - Review
PY - 2009
SP - 924-937
ST - Nanosilver-The burgeoning therapeutic molecule and its green synthesis
T2 - Biotechnology Advances
TI - Nanosilver-The burgeoning therapeutic molecule and its green synthesis
70349781652&doi=10.1016%2fj.biotechadv.2009.08.001&partnerID=40&md5=ef6ce27ba676a85
e48e3f5e5ec3d4bf9
VL - 27
ID - 5796
ER -
TY - JOUR
AB - Nanotechnology is a field that is burgeoning day by day. making an impact in
all spheres of human life. Biological methods of synthesis have paved way for the
"greener synthesis" of nanoparticles and these have proven to be better methods due
to slower kinetics, they offer better manipulation and control over crystal growth
and their stabilization. This has motivated an upsurge in research on the synthesis
routes that allow better control of shape and size for various nanotechnological
applications Nanosilver has developed as a potent antibacterial. antifungal, anti-
viral and anti-inflammatory agent The recent advancement in the Field includes the
enzymatic method of synthesis suggesting enzymes to be responsible for the
nanoparticle formation The biomedical applications of silver nanoparticle can be
effective by the Use of biologically synthesized nanoparticles which minimize the
factors such as toxicity and cost and are found to be exceptionally stable. The
targeting of cancer cells using silver nanoparticles has proven to be effective,
but neither the exact mechanism of action nor the modes of activation of the
downstream signaling molecules have been revealed yet The review illustrates a
probable signaling pathway and mechanism by which silver nanoparticles target the
cancer cells. The current review also examines the historical background of
nanoparticles. role of silver nanoparticles in various biomedical applications and
also focusing oil better methods of the synthesis of nanoparticles (C) 2009
Elsevier Inc All rights reserved
AN - WOS:000271554200021
AU - Vaidyanathan, R.
AU - Kalishwaralal, K.
AU - Gopalram, S.
AU - Gurunathan, S.
DA - NOV-DEC
DO - 10.1016/j.biotechadv.2009.08.001
IS - 6
PY - 2009
SN - 0734-9750
1873-1899
SP - 924-937
ST - RETRATADO: Nanosilver-The burgeoning therapeutic molecule and its green
synthesis (Retracted article. See vol. 28, pg. 940, 2010)
T2 - BIOTECHNOLOGY ADVANCES
TI - RETRATADO: Nanosilver-The burgeoning therapeutic molecule and its green
synthesis (Retracted article. See vol. 28, pg. 940, 2010)
VL - 27
ID - 6035
ER -
TY - JOUR
AB - Eosinophilic inflammation is frequently observed in response to nanoparticle
(NP) exposure in airway rodent models of allergies where the number of eosinophils
is increased in lungs. Despite this, it is surprising that the potential cytotoxic
effect of NP, as well as their direct role on eosinophils is poorly documented. The
present study investigated how different NP can alter the biology of the human
eosinophilic cell line AML14.3D10. It was found that among NP forms of CeO2, ZnO,
TiO2, and nanosilver of 20 nm (AgNP20) or 70 nm (AgNP70) diameters, only ZnO and
AgNP20 induced apoptosis. Caspases-7 and -9 were not activated by the tested NP
while caspase-3 was activated by AgNP20 only. However, both ZnO and AgNP20 induced
cytoskeletal breakdown as evidenced by the cleavage of lamin B1. Using an ELISArray
approach for the simultaneous detection of several analytes (cytokines/chemokines),
it was found that only ZnO and AgNP20 increased the production of different
analytes including the potent pro-inflammatory CXCL8 (IL-8) chemokine. From the
data here, we conclude that toxic effects of some NP could be observed in human
eosinophil-like cells and that this could be related, at least partially, by
induction of apoptosis and production of cytokines and chemokines involved in
inflammation. The results of this study also indicate that distinct NP do not
activate similarly human eosinophils, since ZnO and AgNP20 induce apoptosis and
cytokine production while others such as TiO2, CeO2, and AgNP70 do not. © 2016
AU - Vallières, F.
AU - Simard, J. C.
AU - Noël, C.
AU - Lavastre, V.
AU - Girard, D.
DB - Scopus
DO - 10.1080/1547691X.2016.1203379
IS - 6
KW - Apoptosis
cytokines
cytoskeleton
eosinophils
nanoparticles
Animals
Caspase 3
Cell Line
Cytoskeleton
Eosinophils
Humans
Immunomodulation
Lamin Type B
Metal Nanoparticles
Mice
Proteolysis
Rats
Silver
Zinc
caspase 3
caspase 7
caspase 9
interleukin 8
ITAC protein
lamin B
lamin b1
protein
RANTES
silver nanoparticle
unclassified drug
zinc oxide
lamin B1
metal nanoparticle
silver
zinc
apoptosis
Article
cell viability
controlled study
cytokine production
enzyme activation
eosinophil
human
human cell
priority journal
protein degradation
zeta potential
animal
cell line
immunology
immunomodulation
metabolism
mouse
rat
M3 - Article
PY - 2016
SP - 817-826
ST - Activation of human AML14.3D10 eosinophils by nanoparticles: Modulatory
activity on apoptosis and cytokine production
TI - Activation of human AML14.3D10 eosinophils by nanoparticles: Modulatory
84978134362&doi=10.1080%2f1547691X.2016.1203379&partnerID=40&md5=3cf9f0a496348ef6ea
1ea144e1d62106
VL - 13
ID - 5543
ER -
TY - JOUR
AB - Eosinophilic inflammation is frequently observed in response to nanoparticle
(NP) exposure in airway rodent models of allergies where the number of eosinophils
is increased in lungs. Despite this, it is surprising that the potential cytotoxic
effect of NP, as well as their direct role on eosinophils is poorly documented. The
present study investigated how different NP can alter the biology of the human
eosinophilic cell line AML14.3D10. It was found that among NP forms of CeO2, ZnO,
TiO2, and nanosilver of 20nm (AgNP20) or 70nm (AgNP70) diameters, only ZnO and
AgNP20 induced apoptosis. Caspases-7 and -9 were not activated by the tested NP
while caspase-3 was activated by AgNP20 only. However, both ZnO and AgNP20 induced
cytoskeletal breakdown as evidenced by the cleavage of lamin B-1. Using an
ELISArray approach for the simultaneous detection of several analytes
(cytokines/chemokines), it was found that only ZnO and AgNP20 increased the
production of different analytes including the potent pro-inflammatory CXCL8 (IL-8)
chemokine. From the data here, we conclude that toxic effects of some NP could be
observed in human eosinophil-like cells and that this could be related, at least
partially, by induction of apoptosis and production of cytokines and chemokines
involved in inflammation. The results of this study also indicate that distinct NP
do not activate similarly human eosinophils, since ZnO and AgNP20 induce apoptosis
and cytokine production while others such as TiO2, CeO2, and AgNP70 do not.
AN - WOS:000390880600008
AU - Vallieres, F.
AU - Simard, J. C.
AU - Noel, C.
AU - Lavastre, V.
AU - Girard, D.
DO - 10.1080/1547691X.2016.1203379
IS - 6
PY - 2016
SN - 1547-691X
1547-6901
SP - 817-826
ST - Activation of human AML14.3D10 eosinophils by nanoparticles: Modulatory
TI - Activation of human AML14.3D10 eosinophils by nanoparticles: Modulatory
VL - 13
ID - 6409
ER -
TY - JOUR
AB - Background: Humans are increasingly exposed via the diet to Ag nanoparticles
(NP) used in the food industry. Because of their anti-bacterial activity, ingested
Ag NP might disturb the gut microbiota that is essential for local and systemic
homeostasis. We explored here the possible impact of dietary Ag NP on the gut
microbiota in mice at doses relevant for currently estimated human intake. Methods:
Mice were orally exposed to food (pellets) supplemented with increasing doses of Ag
NP (0, 46, 460 or 4600 ppb) during 28 d. Body weight, systemic inflammation and gut
integrity were investigated to determine overall toxicity, and feces DNA collected
from the gut were analyzed by Next Generation Sequencing (NGS) to assess the effect
of Ag NP on the bacterial population. Ag NP were characterized alone and in the
supplemented pellets by scanning transmission electron microscopy (STEM) and energy
dispersive X-ray analysis (EDX). Results: No overall toxicity was recorded in mice
exposed to Ag NP. Ag NP disturbed bacterial evenness (alpha-diversity) and
populations (beta-diversity) in a dose-dependent manner. Ag NP increased the ratio
between Firmicutes (F) and Bacteroidetes (B) phyla. At the family level,
Lachnospiraceae and the S24-7 family mainly accounted for the increase in
Firmicutes and decrease in Bacteroidetes, respectively. Similar effects were not
observed in mice identically exposed to the same batch of Ag NP-supplemented
pellets aged during 4 or 8 months and the F/B ratio was less or not modified.
Analysis of Ag NP-supplemented pellets showed that freshly prepared pellets
released Ag ions faster than aged pellets. STEM-EDX analysis also showed that Ag
sulfidation occurred in aged Ag NP-supplemented pellets. Conclusions: Our data
indicate that oral exposure to human relevant doses of Ag NP can induce microbial
alterations in the gut. The bacterial disturbances recorded after Ag NP are similar
to those reported in metabolic and inflammatory diseases, such as obesity. It also
highlights that Ag NP aging in food, and more specifically sulfidation, can reduce
the effects of Ag NP on the microbiota by limiting the release of toxic Ag ions.
AN - WOS:000379264300001
AU - van den Brule, S.
AU - Ambroise, J.
AU - Lecloux, H.
AU - Levard, C.
AU - Soulas, R.
AU - De Temmerman, P. J.
AU - Palmai-Pallag, M.
AU - Marbaix, E.
AU - Lison, D.
C7 - 38
DA - JUL 8
DO - 10.1186/s12989-016-0149-1
PY - 2016
SN - 1743-8977
ST - Dietary silver nanoparticles can disturb the gut microbiota in mice
TI - Dietary silver nanoparticles can disturb the gut microbiota in mice
VL - 13
ID - 6213
ER -
TY - JOUR
AB - Background: Humans are increasingly exposed via the diet to Ag nanoparticles
(NP) used in the food industry. Because of their anti-bacterial activity, ingested
Ag NP might disturb the gut microbiota that is essential for local and systemic
homeostasis. We explored here the possible impact of dietary Ag NP on the gut
microbiota in mice at doses relevant for currently estimated human intake. Methods:
Mice were orally exposed to food (pellets) supplemented with increasing doses of Ag
NP (0, 46, 460 or 4600 ppb) during 28 d. Body weight, systemic inflammation and gut
integrity were investigated to determine overall toxicity, and feces DNA collected
from the gut were analyzed by Next Generation Sequencing (NGS) to assess the effect
of Ag NP on the bacterial population. Ag NP were characterized alone and in the
supplemented pellets by scanning transmission electron microscopy (STEM) and energy
dispersive X-ray analysis (EDX). Results: No overall toxicity was recorded in mice
exposed to Ag NP. Ag NP disturbed bacterial evenness (a-diversity) and populations
(β-diversity) in a dose-dependent manner. Ag NP increased the ratio between
Firmicutes (F) and Bacteroidetes (B) phyla. At the family level, Lachnospiraceae
and the S24-7 family mainly accounted for the increase in Firmicutes and decrease
in Bacteroidetes, respectively. Similar effects were not observed in mice
identically exposed to the same batch of Ag NP-supplemented pellets aged during 4
or 8 months and the F/B ratio was less or not modified. Analysis of Ag NP-
supplemented pellets showed that freshly prepared pellets released Ag ions faster
than aged pellets. STEM-EDX analysis also showed that Ag sulfidation occurred in
aged Ag NP-supplemented pellets. Conclusions: Our data indicate that oral exposure
to human relevant doses of Ag NP can induce microbial alterations in the gut. The
bacterial disturbances recorded after Ag NP are similar to those reported in
metabolic and inflammatory diseases, such as obesity. It also highlights that Ag NP
aging in food, and more specifically sulfidation, can reduce the effects of Ag NP
on the microbiota by limiting the release of toxic Ag ions. © 2016 The Author(s).
AU - van den Brule, S.
AU - Ambroise, J.
AU - Lecloux, H.
AU - Levard, C.
AU - Soulas, R.
AU - De Temmerman, P.
AU - Palmai-Pallag, M.
AU - Marbaix, E.
AU - Lison, D.
C7 - 38
DB - Scopus
DO - 10.1186/s12989-016-0149-1
IS - 1
KW - Bacteria
Dysbiosis
Food
Nanomaterials
NGS
Sulfidation
Toxicity
Animals
Intestines
Metal Nanoparticles
Mice
Microbiota
Microscopy, Electron, Scanning Transmission
Silver
silver nanoparticle
metal nanoparticle
silver
animal experiment
animal model
animal tissue
Article
Bacteroidetes
body weight
controlled study
dietary intake
digestive tract parameters
feces analysis
female
Firmicutes
gut integrity
inflammation
intestine flora
Lachnospiraceae
mouse
next generation sequencing
nonhuman
population abundance
population density
priority journal
toxicity testing
animal
chemistry
dose response
intestine
microbiology
microflora
spectrometry
M3 - Article
PY - 2016
ST - Dietary silver nanoparticles can disturb the gut microbiota in mice
TI - Dietary silver nanoparticles can disturb the gut microbiota in mice
84991746987&doi=10.1186%2fs12989-016-0149-
1&partnerID=40&md5=1edad6c6c2118362092cbb74148ad31d
VL - 13
ID - 5596
ER -
TY - JOUR
AB - Dendritic cells (DCs) can acquire, process, and present antigens to T-cells
to induce an immune response. For this reason, targeting cancer antigens to DCs in
order to cause an immune response against cancer is an emerging area of
nanomedicine that has the potential to redefine the way certain cancers are
treated. The use of plasmonically active silver-coated gold nanorods (henceforth
referred to as plasmonic nano vectors (PNVs)) as potential carriers for DC tumor
vaccines has not been presented before. Effective carriers must be able to be
phagocytized by DCs, present low toxicity, and induce the maturation of DCs-an
early indication of an immune response. When we treated DCs with the PNVs, we found
that the cell viability of DCs was unaffected, up to 200 mu g/ml. Additionally, the
PNVs associated with the DCs as they were phagocytized and they were found to
reside within intracellular compartments such as endosomes. More importantly, the
PNVs were able to induce expression of surface markers indicative of DC activation
and maturation, i. e. CD40, CD86, and MHC class II. These results provide the first
evidence that PNVs are promising carriers for DC-based vaccines and warrant further
investigating for clinical use.
AN - WOS:000405464400001
AU - Vang, K. B.
AU - Safina, I.
AU - Darrigues, E.
AU - Nedosekin, D.
AU - Nima, Z. A.
AU - Majeed, W.
AU - Watanabe, F.
AU - Kannarpady, G.
AU - Kore, R. A.
AU - Casciano, D.
AU - Zharov, V. P.
AU - Griffin, R. J.
AU - Dings, R. P. M.
AU - Biris, A. S.
C7 - 5513
DA - JUL 14
DO - 10.1038/s41598-017-04459-1
PY - 2017
SN - 2045-2322
ST - Modifying Dendritic Cell Activation with Plasmonic Nano Vectors
TI - Modifying Dendritic Cell Activation with Plasmonic Nano Vectors
VL - 7
ID - 6604
ER -
TY - JOUR
AB - It is becoming increasingly clear that nanoparticles (NPs) possess many
potential applications in both clinical medicine and research. Potential
utilization of NPs in nanomedicine for the treatment of respiratory diseases where
eosinophils exert pathogenic roles is gaining increasing attention. Even though
several NPs were found to possess pro-inflammatory activities in in vivo models
based on an increased number of eosinophils in rodent airways, it is not clear how
NPs could directly activate eosinophils themselves and how they can alter their
biology. In this review, we discuss the most recent data in this new area of
research demonstrating that NPs could now be added as new eosinophils modulators.
Indeed, activation of eosinophils with NPs could lead to modulation of spontaneous
apoptosis, caspase activation, and cytoskeleton breakdown when apoptosis is
induced; cytokine production, de novo protein synthesis, cellular adhesion onto a
cell substratum, and cell signalling events such as activation of the
phosphoinositide 3-kinase/Akt pathway and actin re-localization are involved in NP-
induced adhesion. Therefore, future development of therapeutic strategies with NPs
aiming at targeting diseases where eosinophils are involved should now consider the
capacity of NPs to modulate human eosinophil biology.
AN - WOS:000517850100002
AU - Vanharen, M.
AU - Girard, D.
DA - FEB
DO - 10.1007/s10753-019-01064-4
IS - 1
PY - 2020
SN - 0360-3997
1573-2576
SP - 8-16
ST - Activation of Human Eosinophils with Nanoparticles: a New Area of Research
T2 - INFLAMMATION
TI - Activation of Human Eosinophils with Nanoparticles: a New Area of Research
VL - 43
ID - 6559
ER -
TY - JOUR
AB - Wound infections constitute an increasing clinical problem worldwide. To
reverse this trend, several wound dressings with antimicrobial properties have been
developed. Considering the increasing presence of antibiotic-resistant
microorganisms, product developers have been focusing their efforts in introducing
antibiotic-free antibacterial wound dressings to the market, with silver being the
most commonly incorporated antimicrobial agent. In this scenario, gaining
information about the microbial and eukaryotic cells' response to these dressings
is needed for a proper selection of antimicrobial dressings for the different cases
of infected wounds. In particular, one insufficiently explored parameter is the
effect of the dressings on the immunomodulation of macrophages, the main immune
cell population participating in the repair process, because of their pivotal role
in the transition of the inflammation to the proliferation phase of wound healing.
In this work, three different clinically applied antimicrobial, silver impregnated
wound dressings were selected: Atrauman(R) Ag, Biatain(R) Alginate Ag and PolyMem
WIC Silver(R) Non-adhesive. Antimicrobial susceptibility tests (disk diffusion and
broth dilution), cell viability evaluation (CellTiter-Blue(R)) and experiments to
determine macrophage polarization (e.g., flow cytometry, ELISA and glucose uptake)
were performed after 24 h of incubation. Among all products tested, Biatain(R)
Alginate Ag induced the most evident bactericidal effect on Gram-positive and Gram-
negative bacteria, followed by PolyMem WIC Silver(R) Non-adhesive, but did not show
good cytocompatibility in vitro. On the other hand, Atrauman(R) Ag showed excellent
cytocompatibility on L929 fibroblasts, HaCaT keratinocytes and THP-1 derived
macrophages, but no significant antimicrobial activity was observed. Overall, it
was confirmed that macrophages initiate, in fact, an alteration of their metabolism
and phenotype in response to wound dressings of different composition in a short
period of contact (24 h). M0 resting state macrophages common response to all
silver-containing dressings used in this study was to increase the production of
the anti-inflammatory cytokine TGF-beta, which indicates an acquisition of M2-like
macrophages characteristics.
AN - WOS:000525053900001
AU - Varela, P.
AU - Marlinghaus, L.
AU - Sartori, S.
AU - Viebahn, R.
AU - Salber, J.
AU - Ciardelli, G.
C7 - 124
DA - FEB 25
DO - 10.3389/fbioe.2020.00124
PY - 2020
SN - 2296-4185
ST - Response of Human Macrophages to Clinically Applied Wound Dressings Loaded
With Silver
TI - Response of Human Macrophages to Clinically Applied Wound Dressings Loaded
With Silver
VL - 8
ID - 6331
ER -
TY - JOUR
AB - Oral mucositis (OM) is among the most common, painful, and debilitating
toxicities of cancer regimen-related treatment, resulting in the formation of
ulcers, which are susceptible to increased colonization of microorganisms. Novel
discoveries in OM have focused on understanding the host-microbial interactions,
because current pathways have shown that major virulence factors from
microorganisms have the potential to contribute to the development of OM and may
even prolong the existence of already established ulcerations, affecting tissue
healing. Additional comprehensive and disciplined clinical investigation is needed
to carefully characterize the relationship between the clinical trajectory of OM,
the local levels of inflammatory changes (both clinical and molecular), and the ebb
and flow of the oral microbiota. Answering such questions will increase our
knowledge of the mechanisms engaged by the oral immune system in response to
mucositis, facilitating their translation into novel therapeutic approaches. In
doing so, directed clinical strategies can be developed that specifically target
those times and tissues that are most susceptible to intervention.
AN - WOS:000379555200001
AU - Vasconcelos, R. M.
AU - Sanfilippo, N.
AU - Paster, B. J.
AU - Kerr, A. R.
AU - Li, Y.
AU - Ramalho, L.
AU - Queiroz, E. L.
AU - Smith, B.
AU - Sonis, S. T.
AU - Corby, P. M.
DA - JUL
DO - 10.1177/0022034516641890
IS - 7
PY - 2016
SN - 0022-0345
1544-0591
SP - 725-733
ST - Host-Microbiome Cross-talk in Oral Mucositis
T2 - JOURNAL OF DENTAL RESEARCH
TI - Host-Microbiome Cross-talk in Oral Mucositis
VL - 95
ID - 6773
ER -
TY - JOUR
AB - The review summarizes the recent data on the toxic effect that engineered
metal nanoparticles (NPs) and their oxides (Ag, Au, Cu, CuO, zero-valent iron,
TiO2, and ZnO) exert on marine organisms of various taxonomic groups. Toxicity is
considered with due regard of the size effects and physicochemical properties of
NPs. Available data indicate that metallic NPs may adversely affect algae,
bacteria, mollusks, worms, arthropods, echinoderms, chordates, etc. Toxic effects
vary from reproductive disorders to death. Only scarce data are available on the NP
effect on marine vertebrates (e.g., marine fish). The factors that affect the NP
toxicity in an aqueous environment include the physicochemical properties of NPs,
species specificities of the test organism, and parameters of the aqueous
environment (in particular, salinity, temperature and pH). Exact relationships
between the factors and NP toxicity are still poorly understood. In particular,
there is no consensus on how salinity affects the NP toxicity in an aqueous
environment. Putative mechanisms of NP toxicity include oxidative stress, impaired
cell membrane integrity, inflammatory processes with consequent cell dysfunction,
DNA damage, and genetic mutations. NPs easily persist within organisms and
consequently have a high potential to accumulate in marine food chains. Lack of
standardized techniques to detect metallic and metal oxide NPs and weak or absent
relevant legislation complicate toxicology studies in marine ecosystems. The above
circumstances make it necessary to further evaluate the safety of metallic NPs for
marine organisms and especially vertebrates, to study the distant effects
associated with NP transition and accumulation in marine food chains, and to unify
the respective research protocols. The exact role of environmental factors in
modulating NP toxicity and the mechanisms of NP toxicity are also important issues
to address in future studies.Y
AN - WOS:000675268900002
AU - Chaika, V. V.
AU - Pikula, K. S.
AU - Golokhvast, K. S.
AU - Gusev, A. A.
DA - MAR
DO - 10.1134/S2635167621020178
IS - 2
PY - 2021
SN - 2635-1676
2635-1684
SP - 138-154
ST - Toxic Effect of Metal-Based Nanomaterials on Representatives of Marine
Ecosystems: A Review
T2 - NANOBIOTECHNOLOGY REPORTS
TI - Toxic Effect of Metal-Based Nanomaterials on Representatives of Marine
Ecosystems: A Review
VL - 16
ID - 6754
ER -
TY - JOUR
AB - ABSTRACT Tooth bleaching is a technique of choice to obtain a harmonious
smile, but bleaching agents may damage the dental pulp. Objective: This study
evaluated the inflammatory responses of human dental pulp after the use of two
bleaching techniques. Material and Methods: Pulp samples were collected from human
third molars extracted for orthodontic reasons and divided into three groups:
control - no tooth bleaching (CG) (n=7); at-home bleaching with 15% carbamide
peroxide (AH) (n = 10), and in-office bleaching with 38% hydrogen peroxide (IO)
(n=12). Pulps were removed and stained with hematoxylin-eosin for microscopic
analysis of inflammation intensity, collagen degradation, and pulp tissue
organization. Immunohistochemistry was used to detect mast cells (tryptase+), blood
vessels (CD31+), and macrophages (CD68+). Chi-square, Kruskal-Wallis, and Mann
Whitney tests were used for statistical analysis. The level of significance was set
at p<.05. Results: The inflammation intensity and the number of macrophages were
significantly greater in IO than in AH and CG (p<0.05). The results of CD31+ (blood
vessels per mm2) were similar in CG (61.39±20.03), AH (52.29±27.62), and IO
(57.43±8.69) groups (p>0.05). No mast cells were found in the pulp samples
analyzed. Conclusion: In-office bleaching with 38% hydrogen peroxide resulted in
more intense inflammation, higher macrophages migration, and greater pulp damage
then at-home bleaching with 15% carbamide peroxide, however, these bleaching
techniques did not induce migration of mast cells and increased the number of blood
vessels.
AD - Vaz, Maysa Magalhães
Universidade Federal de Goiás. Goiânia. BR
Lopes, Lawrence Gonzaga
Cardoso, Paula Carvalho
Souza, João Batista de
Batista, Aline Carvalho
Costa, Nádia Lago
Torres, Érica Miranda
Estrela, Carlos
AU - Vaz, Maysa Magalhães
AU - Lopes, Lawrence Gonzaga
AU - Cardoso, Paula Carvalho
AU - Souza, João Batista de
AU - Batista, Aline Carvalho
AU - Costa, Nádia Lago
AU - Torres, Érica Miranda
AU - Estrela, Carlos
C1 - 20161107
DA - 2016/10
DB - LILACS
DO - 10.1590/1678-775720160137
IS - 5
KW - Dental pulp
Inflammation
Microscopy
Tooth bleaching
LA - en
PY - 2016
SN - 1678-7757
SP - 509-517
ST - Inflammatory response of human dental pulp to at-home and in-office tooth
bleaching
TI - Inflammatory response of human dental pulp to at-home and in-office tooth
bleaching
77572016000500509
VL - 24
ID - 4930
ER -
TY - JOUR
AB - Pulmonary fibrosis is characterized by an inflammatory response that includes
macrophages, neutrophils, lymphocytes, and mast cells. The purpose of this study
was to evaluate whether mast cells play a role in initiating pulmonary fibrosis.
Pulmonary fibrosis was induced with bleomycin in mast-cell-deficient WBB6F1-W/W-v
(MCD) mice and their congenic controls (WBB6F1-(+)/(+)). Mast cell deficiency
protected against bleomycin-induced pulmonary fibrosis, but protection was reversed
with the re-introduction of mast cells to the lungs of MCD mice. Two mast cell
mediators were identified as fibrogenic: histamine and renin, via angiotensin (ANG
II). Both human and rat lung fibroblasts express the histamine H-1 and ANG II AT(1)
receptor subtypes and when activated, they promote proliferation, transforming
growth factor beta(1) secretion, and collagen synthesis. Mast cells appear to be
critical to pulmonary fibrosis. Therapeutic blockade of mast cell degranulation
and/or histamine and ANG II receptors should attenuate pulmonary fibrosis.
AN - WOS:000317406900009
AU - Veerappan, A.
AU - O'Connor, N. J.
AU - Brazin, J.
AU - Reid, A. C.
AU - Jung, A.
AU - McGee, D.
AU - Summers, B.
AU - Branch-Elliman, D.
AU - Stiles, B.
AU - Worgall, S.
AU - Kaner, R. J.
AU - Silver, R. B.
DA - APR
DO - 10.1089/dna.2013.2005
IS - 4
PY - 2013
SN - 1044-5498
1557-7430
SP - 206-218
ST - Mast Cells: A Pivotal Role in Pulmonary Fibrosis
T2 - DNA AND CELL BIOLOGY
TI - Mast Cells: A Pivotal Role in Pulmonary Fibrosis
VL - 32
ID - 6695
ER -
TY - JOUR
AB - The present investigation highlights a unique, rapid, greener, economical and
biological approach in the fabrication of gold (Au), copper (Cu), iron (Fe) and
zinc (Zn) monometallic nanoparticles (MNPs) using Annona muricata aqueous leaf
extract (AMLE) as a suitable reducing, capping and stabilizing agent. The bio-
fabricated NPs were characterized using UV-Visible spectrophotometer and field
emission scanning electron microscope with energy-dispersive x-ray spectroscopy
(FESEM-EDX). Surface plasmon resonance (SPR) determined the formation of MNPs with
maximum absorbance at 540, 570, 280 and 360 nm for Au, Cu, Fe and Zn-NPs
respectively. Among all the bio-fabricated MNPs investigated CuNPs have showed
superior activity for their antibacterial (against Escherichia coli), anti-
inflammatory (egg albumin protein denaturation inhibition-51.46 +/- 1.53 mu g/mL),
antidiabetic (alpha-glucosidase activity inhibition-32.97 +/- 2.51 mu g/mL), anti-
oxidative (DPPH free radical inhibition-63.44 +/- 3.12 mu g/mL) and anti-cancer
(against K562 cancer cell line using MTT assay-53.34 +/- 2.29 mu g/mL) potentials.
Whereas ZnNPs showed less-activity in anti-inflammatory and anti-oxidative (> 100
mu g/mL) and no activity was observed in anti-diabetic and anti-cancer assays
compared to other investigated NPs. Both Cuand Fe-NPs showed catalytic ability in
the degradation of textile dyes in the presence of sodium borohydride (NaBH4). Cu
and Fe-NPs degraded 94% of rhodamine-B (rh-B) under 35 and 90 min, whereas 94% of
methyl orange (MO) under 25 and 70 min, respectively. Eco-toxicity studies carried
out using Artemia nauplii have suggested that ZnNPs are less toxic (LC50 was > 100
mu g/mL after 24 h) compared to other investigated NPs.
AN - WOS:000568768200002
AU - Velidandi, A.
AU - Pabbathi, N. P. P.
AU - Dahariya, S.
AU - Baadhe, R. R.
C7 - 100302
DA - SEP
DO - 10.1016/j.colcom.2020.100302
PY - 2020
SN - 2215-0382
ST - Catalytic and eco-toxicity investigations of bio-fabricated monometallic
nanoparticles along with their anti-bacterial, anti-inflammatory, anti-diabetic,
anti-oxidative and anti-cancer potentials
T2 - COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
TI - Catalytic and eco-toxicity investigations of bio-fabricated monometallic
nanoparticles along with their anti-bacterial, anti-inflammatory, anti-diabetic,
anti-oxidative and anti-cancer potentials
VL - 38
ID - 6216
ER -
TY - JOUR
AB - There is a need for developing eco-friendly, cost-effective materials in
medical, biological, and agricultural fields, now, the problem can be resolved
using environment-friendly green approached metal oxide nanoparticles were used.
Therefore, the CuO nanoparticles were biosynthesized using Panicum sumatrense
grains aqueous extract for the first time. The gas chromatography-mass spectrometry
analysis revealed the diethyl phthalate (99.18%) was the major active phytocompound
in the grains extract. The biosynthesized CuO nanoparticles were characterized
using various analytical techniques. The UV-visible spectrum revealed the surface
Plasmon resonance band of CuO nanoparticles at 305 nm. X-ray diffraction pattern
showed the high crystalline nature of nanoparticles and their mean crystallite size
was at 25 nm. Fourier transform infra-red spectrum exposed the Cu-O stretching
vibrational bands at 830 and 510 cm(-1). The high resolution transmission electron
micrographs illustrated the cluster of rectangular shaped nanoparticles and their
average particle size was in between 15 and 35 nm. The energy dispersive spectrum
and mapping analyses showed the major presence of Cu and O in nanoparticles. The
antibacterial activity and its SEM images revealed the bactericidal characteristics
of CuO nanoparticles against Salmonella typhi and Staphylococcus aureus bacteria at
150 mu g/mL concentration. The maximum zone of inhibition of nanoparticles against
S. typhi and S. aureus bacterial cells were 22 and 26 mm, respectively. The
antioxidant, anti-inflammatory, and anti-diabetic analyses indicated the effectual
biological activities at 98.25, 92.65, and 89.10% in 80 mu g/mL concentration of
CuO nanoparticles, respectively. The morphological and tissue changes in Culex
tritaeniorhynchus mosquito larvae and Tribolium castaneum insect revealed the
effective larvicidal and insecticidal activity of CuO nanoparticles. The maximum
mortality of larvae and insect by CuO nanoparticles were 100 ppm at 48 h and 100 mu
g/Kg at 48, 72 h, 75 mu g/Kg at 72 h, respectively. The anticancer activity
divulged the effectual inhibition (95.69%) of CuO nanoparticles against MCF-7
breast cancer cells in low IC50 value at 11.49 mu g/mL. The activity against L929
(fibroblast) normal cells showed non-toxicity of CuO nanoparticles to normal cells.
The plant growth analysis explained the efficient growth of Vigna mungo plant at 60
ppm concentration of CuO nanoparticles. As per the results, the synthesized CuO
nanoparticles can be applied as a biocompatible, cost-effective insecticides, drugs
and fertilizers.
AN - WOS:000767920800001
AU - Velsankar, K.
AU - Parvathy, G.
AU - Mohandoss, S.
AU - Kumar, R. M.
AU - Sudhahar, S.
C6 - MAR 2022
DA - JUN
DO - 10.1007/s13204-022-02441-6
IS - 6
PY - 2022
SN - 2190-5509
2190-5517
SP - 1993-2021
ST - Green synthesis and characterization of CuO nanoparticles using Panicum
sumatrense grains extract for biological applications
T2 - APPLIED NANOSCIENCE
TI - Green synthesis and characterization of CuO nanoparticles using Panicum
sumatrense grains extract for biological applications
VL - 12
ID - 6667
ER -
TY - JOUR
AB - Iron oxide nanoparticles have been used in diverse fields due to their
significant applications. The synthesis of iron oxide nanoparticles using plant
material extract is a simple, inexpensive and eco-friendly approach. The present
study demonstrates the effective bio-synthesis of iron oxide nanoparticles using
grains extract of Echinochloa frumentacea. GC-MS analysis report revealed the
information about the phytochemicals in aqueous grains extract. The formation of
iron oxide nanoparticles was confirmed using UV-visible, XRD, FTIR, HRTEM, EDX, and
mapping analyses. The UV-visible spectrum of iron oxide nanoparticles showed the
SPR band at 289 nm. The XRD studies confirmed the formation of high crystalline
iron oxide nanoparticles. HRTEM analysis showed that the formed nanoparticles were
rectangular and triangular in shape and the size ranged between 20 and 40 nm. The
iron oxide nanoparticles revealed significant antibacterial activity against the
microbes such as Staphylococcus aureus and Salmonella typhi at 150 mu g/mL and
morphological changes were observed. The high concentration of nanoparticles
exhibited 24 and 27 mm zone of inhibition on S. typhi and S. aureus, respectively.
We observed the role of biological activities such as antioxidant, anti-
inflammatory, and anti-diabetic properties of nanoparticles, and they showed
excellent bioactive properties even at a low IC50 value. The antioxidant,
antiinflammatory and anti-diabetic analyses showed high activity at 95.10, 92.10
and 91.68% for 80 mu g/mL concentration, respectively. Moreover, the iron oxide
nanoparticles exhibited high cytotoxicity (94.36%) against A549 cancer cells and no
toxicity against HEK293 normal cells which showed the biocompatibility of
nanoparticles. The larvicidal and insecticidal activities of nanoparticles showed
the maximum mortality at 48 h in 100 ppm and 72 h in 75, 100 mu g/kg, respectively.
The morphological and structural changes in larvae and insects validated the
effective biological activities of iron oxide nanoparticles. The plant growth study
results recommended that the lower concentration of nanoparticles at 60 ppm was
helpful for the germination of seeds and growth of root and stem in the Vigna
unguiculata plant. Finally, the study results suggested that the synthesized iron
oxide nanoparticles can potentially be useful in pharmaceutical, agricultural,
targeted drug delivery and biomedical applications.
AN - WOS:000857219400002
AU - Velsankar, K.
AU - Parvathy, G.
AU - Mohandoss, S.
AU - Ravi, G.
AU - Sudhahar, S.
C6 - SEP 2022
C7 - 103799
DA - OCT
DO - 10.1016/j.jddst.2022.103799
PY - 2022
SN - 1773-2247
2588-8943
ST - Echinochloa frumentacea grains extract mediated synthesis and
characterization of iron oxide nanoparticles: A greener nano drug for potential
TI - Echinochloa frumentacea grains extract mediated synthesis and
characterization of iron oxide nanoparticles: A greener nano drug for potential
VL - 76
ID - 6546
ER -
TY - JOUR
AB - In this report, the silver oxide nanoparticles were green synthesized using
Panicum miliaceum grains extract and were proposed for the first time. GC–MS
analysis explicated 2-Acetylbenzoic acid was the active phytocompound with 97.07%
of presence in aqueous grains extract. The synthesized silver oxide nanoparticles
were analyzed by several analytical techniques such as UV–visible, XRD, FT-IR, HR-
TEM, TG, XPS, EDX and mapping analyses. The results of various analytical
techniques confirmed the silver oxide nanoparticles formation. The formed
nanoparticles were in 10–25 nm size. The effectual bioactive properties of
nanoparticles were revealed through antioxidant, anti-diabetic, anti-inflammatory,
larvicidal and insecticidal activities. The high mortality of larvae and insect was
observed at 48 h in 100 ppm and 72 h in 100 μg/Kg concentration, respectively. The
antibacterial activity explained the bactericidal property of nanoparticles on S.
aureus and S. typhi at 150 μg/mL concentration. The effective drug activity of
nanoparticles was observed from 98.10 % of toxicity against A549 lung cancer cells
at 100 μg/mL concentration. The growth of Vigna unguiculata was efficiently
increased by lower concentration (60 ppm) of nanoparticles. According to results,
the green synthesized nanoparticles can be applied in pharmaceutical and
agricultural sectors as biocompatible, non-toxic and cost-effective material. ©
2022 The Society of Powder Technology Japan
AU - Velsankar, K.
AU - Parvathy, G.
AU - Sankaranarayanan, K.
AU - Mohandoss, S.
AU - Sudhahar, S.
C7 - 103645
DB - Scopus
DO - 10.1016/j.apt.2022.103645
IS - 7
Green synthesis
Larvicidal activity
Rectangular shape
Silver oxide nanoparticles
Biocompatibility
Drug delivery
Grain (agricultural product)
Grain size and shape
Oxides
Silver oxides
Biological applications
GC/MS analysis
Larvicidal activities
Panicum
Rectangular shapes
Synthesised
UV-visible
Cost effectiveness
M3 - Article
PY - 2022
ST - Green synthesis of silver oxide nanoparticles using Panicum miliaceum grains
extract for biological applications
T2 - Advanced Powder Technology
TI - Green synthesis of silver oxide nanoparticles using Panicum miliaceum grains
extract for biological applications
85131404706&doi=10.1016%2fj.apt.2022.103645&partnerID=40&md5=bea535d9c6424ba5ed262a
0693dcc3bd
VL - 33
ID - 5128
ER -
TY - JOUR
AB - Purpose: Pathogenesis of Acanthamoeba keratitis involves breakdown of
epithelial barrier, stromal invasion by Acanthamoeba, loss of keratocytes,
inflammatory response and finally stromal necrosis. The loss of keratocytes,
believed to be due to the phagocytic activity of the parasite, occurs
disproportionate to and independent of the parasite load, thereby suggesting
additional modes of cell loss. To test our hypothesis that the loss of keratocytes
in Acanthamoeba keratitis is due to apoptosis, we did both histology and
histochemistry on the corneal tissues. Methods: Routine Haematoxylin and Eosin,
Gomori's Methenamine Silver and Periodic acid Schiff stained sections of five
corneal tissues from penetrating keratoplasty and eviscerated eyes were reviewed.
TUNEL staining was done for morphological detection of apoptosis in three cases,
using formalin-fixed, paraffin-processed tissues. Results: Histological changes
were epithelial ulceration, loss of keratocytes in all layers, inflammation in
anterior two-thirds of the stroma with necrosis, and deeper quiet stroma.
Acanthamoeba trophozoites were found in the anterior stroma while the cysts were
more in the deeper stroma, with minimal or no inflammatory response. TUNEI staining
was positive in keratocytic nuclei in all layers. Conclusions: This study
demonstrates that one of the modes of keratocyte loss in Acanthamoeba keratitis is
by apoptosis, possibly in addition to the necrotic process and phagocytic activity
of the parasite. The death of inflammatory cells also appears to be mediated by
apoptosis.
AU - Vemuganti, G. K.
AU - Sharma, S.
AU - Athmanathan, S.
AU - Garg, P.
DB - Scopus
IS - 4
KW - Acanthamoeba keratitis
Apoptosis
Histopathology
TUNEL stain
Acanthamoeba
Acanthamoeba Keratitis
Animals
Corneal Stroma
DNA
Eye Evisceration
Humans
Keratoplasty, Penetrating
Necrosis
Phagocytosis
animal
apoptosis
article
cornea stroma
evaluation
eye surgery
genetics
human
necrosis
nick end labeling
parasitology
pathology
penetrating keratoplasty
phagocytosis
M3 - Article
PY - 2000
SP - 291-294
ST - Keratocyte loss in Acanthamoeba keratitis: Phagocytosis, necrosis or
apoptosis ?
T2 - Indian Journal of Ophthalmology
TI - Keratocyte loss in Acanthamoeba keratitis: Phagocytosis, necrosis or
apoptosis ?
0034576968&partnerID=40&md5=27f4c38828f0ea3eb31630e8b2255a29
VL - 48
ID - 5770
ER -
TY - JOUR
AB - Oxidative stress and chronic inflammation interplay with the pathogenesis of
cancer. Breast cancer in women is the burning issue of this century, despite
chemotherapy and magnetic therapy. The management of secondary complications
triggered by post-chemotherapy poses a great challenge. Thus, identifying target-
specific drugs with anticancer potential without secondary complications is a
challenging task for the scientific community. It is possible that green technology
has been employed in a greater way in order to fabricate nanoparticles by
amalgamating plants with medicinal potential with metal oxide nanoparticles that
impart high therapeutic properties with the least toxicity. Thus, the present study
describes the synthesis of Titanium dioxide nanoparticles (TiO2 NPs) using aqueous
Terenna asiatica fruit extract, with its antioxidant, anti-inflammatory and
anticancer properties. The characterisation of TiO2 NPs was carried out using a
powdered X-ray diffractometer (XRD), Fourier transform infrared (FTIR), scanning
electron microscopy (SEM), energy-dispersive X-ray diffraction (EDX), high-
resolution transmission electron microscopy (HR-TEM), dynamic light scattering
(DLS), and zeta-potential. TiO2 NPs showed their antioxidant property by scavenging
1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals in a dose-dependent manner with
an IC50 value of 80.21 & mu;g/& mu;L. To ascertain the observed antioxidant
potential of TiO2 NPs, red blood cells (RBC) were used as an in vitro model system.
Interestingly, TiO2 NPs significantly ameliorated all the stress parameters, such
as lipid peroxidation (LPO), protein carbonyl content (PCC), total thiol (TT),
superoxide dismutase (SOD), and catalase (CAT) in sodium nitrite (NaNO2)-induced
oxidative stress, in RBC. Furthermore, TiO2 NPs inhibited RBC membrane lysis and
the denaturation of both egg and bovine serum albumin, significantly in a dose-
dependent manner, suggesting its anti-inflammatory property. Interestingly, TiO2
NPs were found to kill the MCF-7 cells as a significant decrease in cell viability
of the MCF-7 cell lines was observed. The percentage of growth inhibition of the
MCF-7 cells was compared to that of untreated cells at various doses (12.5, 25, 50,
100, and 200 & mu;g/mL). The IC50 value of TiO2 NPs was found to be (120 &
mu;g/mL). Furthermore, the Annexin V/PI staining test was carried out to confirm
apoptosis. The assay indicated apoptosis in cancer cells after 24 h of exposure to
TiO2 NPs (120 & mu;g/mL). The untreated cells showed no significant apoptosis in
comparison with the standard drug doxorubicin. In conclusion, TiO2 NPs potentially
ameliorate NaNO2-induced oxidative stress in RBC, inflammation and MCF-7 cells
proliferation.
AN - WOS:001030961400001
AU - Venkatappa, M. M.
AU - Udagani, C.
AU - Gowda, S. H.
AU - Venkataramaiah, S.
AU - Casini, R.
AU - Moussa, I. M.
AU - Achur, R.
AU - Sannaningaiah, D.
AU - Elansary, H. O.
C7 - 5126
DA - JUL
DO - 10.3390/molecules28135126
IS - 13
PY - 2023
SN - 1420-3049
ST - Green Synthesised TiO2 Nanoparticles-Mediated Terenna asiatica: Evaluation of
Their Role in Reducing Oxidative Stress, Inflammation and Human Breast Cancer
Proliferation
T2 - MOLECULES
TI - Green Synthesised TiO2 Nanoparticles-Mediated Terenna asiatica: Evaluation of
Their Role in Reducing Oxidative Stress, Inflammation and Human Breast Cancer
Proliferation
VL - 28
ID - 6482
ER -
TY - JOUR
AB - Curcuma Tonga (turmeric) has a long history of use in medicine as a treatment
for inflammatory conditions. The primary active constituent of turmeric and the one
responsible for its vibrant yellow color is curcumin. Curcumin is used for
treatment of wound and inflammation. It had antimicrobial and antioxidant property.
It has low intrinsic toxicity and magnificent properties like with comparatively
lesser side-effects. Cotton cloth is one of the most successful wound dressings
which utilize the intrinsic properties of cotton fibers. Modern wound dressings,
however, require other properties such as antibacterial and moisture maintaining
capabilities. In this study, conventional cotton cloth was coated with Curcumin
composite for achieving modern wound dressing properties. Curcumin nanocomposite is
characterized. The results show that coated cotton cloth with Curcumin
nanocomposite has increased drying time (74%) and water absorbency (50%).
Furthermore, they show antibacterial efficiency against bacterial species present
in wounds. (C) 2017 Elsevier B.V. All rights reserved.
AN - WOS:000396950600042
AU - Venkatasubbu, G. D.
AU - Anusuya, T.
DA - MAY
DO - 10.1016/j.ijbiomac.2017.02.002
PY - 2017
SN - 0141-8130
1879-0003
SP - 366-378
ST - Investigation on Curcumin nanocomposite for wound dressing
TI - Investigation on Curcumin nanocomposite for wound dressing
VL - 98
ID - 6787
ER -
TY - CONF
AB - Silver nanoparticles were found to have an excellent antimicrobial activity
and green synthesis enhance the activity of Silver nanoparticles. Mimosa pudica
commonly called as touch me-not plant has found to have antimicrobial, anti-
oxidant, anti-inflammatory and antiseptic properties. Ormocarpum cochinchinense
commonly called as 'elumbotti' is a shrub which is traditionally used for bone
fixing in the areas like Tamilnadu.Hydroxyapatite is one of the popular biomaterial
which has good biocompatibility commonly used in the field of dentistry and
Orthopaedics. Osteosarcoma or Osteogenic sarcoma is a primary malignant bone tumour
commonly affects children and adolescents. Recently, Hydroxyapatite was found to
have proliferative-suppressive effect on osteosarcoma cells. The leaves of Mimosa
pudica and ormocarpum cochin chinense were shadow dried and its aqueous extract was
prepared. The Silver nano particles were synthesized from both the plants and the
UV-visible spectrum shows good stability in mimosa pudica sample which was taken
for further use. Further the sample was characterized by Transmission Electron
Microscopy (TEM) and Energy dispersive X-ray analysis (EDX), X-ray Diffraction
(XRD).To enhance the drug delivery, synthesized silver nanoparticles were
conjugated with hydroxyapatite powder. The antimicrobial and cytotoxic activity of
the bio-conjugate was evaluated. The in-vitro activity of the bio conjugate will be
evaluated in the osteo sarcoma cell line (MG-63). The produced bio conjugate can be
used in the treatment of osteo sarcoma and in treating bone injuries and bone
inflammations. © 2022 Author(s).
AU - Vennila Preethi, S.
AU - Geetha Gayathri, V.
AU - Jeffrey Calwin, J.
DB - Scopus
DO - 10.1063/5.0105011
KW - Cytotoxicity
Hydroxyapatite
Mimosa pudica
Osteosarcoma cell line (MG-63)
PY - 2022
ST - Synthesis of silver nanoparticles from mimosa pudica and bio-conjugation with
hydroxyapatite for orthopaedic application
T2 - AIP Conference Proceedings
TI - Synthesis of silver nanoparticles from mimosa pudica and bio-conjugation with
hydroxyapatite for orthopaedic application
85140330764&doi=10.1063%2f5.0105011&partnerID=40&md5=a4bb94acfeb6ec8e74d1697fd77087
29
VL - 2518
ID - 5143
ER -
TY - CONF
AB - We observed that cartilage tissue and chondroitin sulfate (CS) can generate
nanoparticles of gold (AuNP) or silver (AgNP) from ionic solutions of Au or Ag.
These observations prompted us to hypothesize that the anti-inflammatory effects
observed during aurotherapy of rheumatoid arthritis might be due to the in vivo
generation of AuNPs. To test this hypothesis we synthesized AuNP or AgNP using
cartilage tissue or CS, and evaluated them in an embryonic zebrafish inflammation
model assay and in a natural killer (NK) cell activity assay. Results from the
zebrafish assays indicate little toxicity from exposure to the AuNP or AgNP
preparations; however, AgNPs proved to be cytotoxic towards the NK cells. The
presence of AuNP or AgNPs decreased the magnitude of the inflammatory response in
zebrafish and the cytotoxic activity of NK cells towards cancer cells.
AU - Vercruysse, K. P.
AU - Harper, S. L.
AU - Ivory, D. M.
AU - Whalen, M. M.
AU - Saili, K. S.
AU - Tanguay, R. L.
DB - Scopus
KW - Cartilage
Chondroitin sulfate
Gold
Inflammation
Silver
Assays
Cells
Commerce
Cytology
Exhibitions
Health
Ligaments
Nanoparticles
Nanostructures
Nanotechnology
Patient treatment
Sulfate minerals
Sulfur compounds
Technology
Anti-inflammatory properties
Cancer cells
Cartilage tissues
Cell activities
Cytotoxic
Cytotoxic activities
In-vivo
Ionic solutions
Life sciences
Natural killer
NK cells
Trade shows
Zebra fish
PY - 2008
SP - 501-504
ST - Potential anti-inflammatory properties of biologically-synthesized
nanoparticles of gold or silver
T2 - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade
Show, NSTI-Nanotech, Nanotechnology 2008
TI - Potential anti-inflammatory properties of biologically-synthesized
52649103975&partnerID=40&md5=e8f6752c066f417f3a850fbb967d22c4
VL - 2
ID - 5782
ER -
TY - CPAPER
AB - We observed that cartilage tissue and chondroitin sulfate (CS) can generate
nanoparticles of gold (AuNP) or silver (AgNP) from ionic solutions of Au or Ag.
These observations prompted us to hypothesize that the anti-inflammatory effects
observed during aurotherapy of rheumatoid arthritis might be due to the in vivo
generation of AuNPs. To test this hypothesis we synthesized AuNP or AgNP using
cartilage tissue or CS, and evaluated them in an embryonic zebrafish inflammation
model assay and in a natural killer (NK) cell activity assay. Results from the
zebrafish assays indicate little toxicity from exposure to the AuNP or AgNP
preparations; however, AgNPs proved to be cytotoxic towards the NK cells. The
presence of AuNP or AgNPs decreased the magnitude of the inflammatory response in
zebrafish and the cytotoxic activity of NK cells towards cancer cells.
AN - WOS:000272169900137
AU - Vercruysse, K. P.
AU - Harper, S. L.
AU - Ivory, D. M.
AU - Whalen, M. M.
AU - Saili, K. S.
AU - Tanguay, R. L.
PY - 2008
SP - 501-504
T2 - NSTI NANOTECH 2008, VOL 2, TECHNICAL PROCEEDINGS
TI - Potential anti-inflammatory properties of biologically-synthesized
ID - 5967
ER -
TY - JOUR
AB - Assessment of nanomaterial (NM) induced inflammatory responses has largely
relied on rodent testing via measurement of leukocyte accumulation in target
organs. Despite observations that NMs activate neutrophil driven inflammatory
responses in vivo, a limited number of studies have investigated neutrophil
responses to NMs in vitro. We compared responses between the human neutrophil-like
HL-60 cell line and human primary neutrophils following exposure to silver (Ag),
zinc oxide (ZnO), copper oxide (CuO) and titanium dioxide (TiO2) NMs. NM
cytotoxicity and neutrophil activation were assessed by measuring cellular
metabolic activity, cytokine production, respiratory burst, and release of
neutrophil extracellular traps. We observed a similar pattern of response between
HL-60 cells and primary neutrophils, however we report that some neutrophil
functions are compromised in the cell line. Ag NMs were consistently observed to
stimulate neutrophil activation, with CuO NMs inducing similar though weaker
responses. TiO2 NMs did not induce a neutrophil response in either cell type.
Interestingly, ZnO NMs readily induced activation of HL-60 cells but did not appear
to activate primary cells. Our findings are relevant to the development of a tiered
testing strategy for NM hazard assessment which promotes the use of non-rodent
models. Whilst we acknowledge that HL-60 cells may not be a perfect substitute for
primary cells and require further investigation regarding their ability to predict
neutrophil activation, we recommend their use for initial screening of NM-induced
inflammation. Primary human neutrophils can then be used for more focused
assessments of neutrophil activation before progressing to in vivo models where
necessary.
AN - WOS:000596367500001
AU - Verdon, R.
AU - Gillies, S. L.
AU - Brown, D. M.
AU - Henry, T.
AU - Tran, L.
AU - Tyler, C. R.
AU - Rossi, A. G.
AU - Stone, V.
C6 - NOV 2020
DA - JAN 2
DO - 10.1080/17435390.2020.1834635
IS - 1
PY - 2021
SN - 1743-5390
1743-5404
SP - 1-20
ST - Neutrophil activation by nanomaterials in vitro: comparing strengths and
limitations of primary human cells with those of an immortalized (HL-60) cell line
T2 - NANOTOXICOLOGY
TI - Neutrophil activation by nanomaterials in vitro: comparing strengths and
VL - 15
ID - 6414
ER -
TY - JOUR
AB - Assessment of nanomaterial (NM) induced inflammatory responses has largely
relied on rodent testing via measurement of leukocyte accumulation in target
organs. Despite observations that NMs activate neutrophil driven inflammatory
responses in vivo, a limited number of studies have investigated neutrophil
responses to NMs in vitro. We compared responses between the human neutrophil-like
HL-60 cell line and human primary neutrophils following exposure to silver (Ag),
zinc oxide (ZnO), copper oxide (CuO) and titanium dioxide (TiO2) NMs. NM
cytotoxicity and neutrophil activation were assessed by measuring cellular
metabolic activity, cytokine production, respiratory burst, and release of
neutrophil extracellular traps. We observed a similar pattern of response between
HL-60 cells and primary neutrophils, however we report that some neutrophil
functions are compromised in the cell line. Ag NMs were consistently observed to
stimulate neutrophil activation, with CuO NMs inducing similar though weaker
responses. TiO2 NMs did not induce a neutrophil response in either cell type.
Interestingly, ZnO NMs readily induced activation of HL-60 cells but did not appear
to activate primary cells. Our findings are relevant to the development of a tiered
testing strategy for NM hazard assessment which promotes the use of non-rodent
models. Whilst we acknowledge that HL-60 cells may not be a perfect substitute for
primary cells and require further investigation regarding their ability to predict
neutrophil activation, we recommend their use for initial screening of NM-induced
inflammation. Primary human neutrophils can then be used for more focused
assessments of neutrophil activation before progressing to in vivo models where
necessary. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor
& Francis Group.
AU - Verdon, R.
AU - Gillies, S. L.
AU - Brown, D. M.
AU - Henry, T.
AU - Tran, L.
AU - Tyler, C. R.
AU - Rossi, A. G.
AU - Stone, V.
DB - Scopus
DO - 10.1080/17435390.2020.1834635
IS - 1
KW - inflammation
in vitro
nanomaterial
nanotoxicology
Neutrophil
Copper
HL-60 Cells
Humans
Inflammation
Nanostructures
Neutrophils
Silver
Titanium
Zinc Oxide
cytochrome c
cytokine
gro alpha
interleukin 8
leukocyte elastase
silver nanoparticle
unclassified drug
copper
cuprous oxide
silver
titanium
titanium dioxide
zinc oxide
Article
cell metabolism
cell structure
cell viability
comparative study
controlled study
cytokine production
cytokine response
cytotoxicity
extracellular trap
hazard assessment
HL-60 cell line
human
human cell
in vitro study
leukocyte function
primary cell
priority journal
respiratory burst
drug effect
neutrophil
M3 - Article
PY - 2021
SP - 1-20
ST - Neutrophil activation by nanomaterials in vitro: comparing strengths and
T2 - Nanotoxicology
TI - Neutrophil activation by nanomaterials in vitro: comparing strengths and
85097041349&doi=10.1080%2f17435390.2020.1834635&partnerID=40&md5=e93626cb9f801043c5
5325b451cd3e54
VL - 15
ID - 5345
ER -
TY - JOUR
AB - A glass belonging to the system SiO2-Al2O 3-CaO-Na2O has been subjected to a
patented ion-exchange treatment to induce surface antibacterial activity by doping
with silver ions. Doped samples have been characterized by means of X-Ray
diffraction (XRD), scanning electron microscopy (SEM) observation, energy
dispersion spectrometry (EDS) analysis, in vitro bioactivity test, Ag+ leaching
test by graphite furnace atomic absorption spectroscopy (GFAAS) analyses,
cytotoxicity tests by fibroblasts adhesion and proliferation, adsorption of IgA and
IgG on to the material to evaluate its inflammatory property and antibacterial
tests (cultures with Staphylococcus aureus and Escherichia coli). In vitro tests
results demonstrated that the modified glass maintains the same biocompatibility of
the untreated one and, moreover, it acquires an antimicrobial action against tested
bacteria. This method can be selected to realize glass or glass-ceramic bone
substitutes as well as coatings on bio-inert devices, providing safety against
bacterial colonization thus reducing the risks of infections nearby the implant
site. The present work is the carrying on of a previous research activity,
concerning the application of an ion-exchange treatment on glasses belonging to the
ternary system SiO2-CaO-Na2O. On the basis of previous results the glass
composition was refined and the ion-exchange process was adapted to it, in order to
tune the final material properties. The addition of Al2O3 to the original glass
system and the optimization of the ion-exchange conditions allowed a better control
of the treatment, leading to an antibacterial material, without affecting both
bioactivity and biocompatibility. © 2008 Springer Science+Business Media, LLC.
AU - Verné, E.
AU - Miola, M.
AU - Vitale Brovarone, C.
AU - Cannas, M.
AU - Gatti, S.
AU - Fucale, G.
AU - Maina, G.
AU - Massé, A.
AU - Di Nunzio, S.
DB - Scopus
DO - 10.1007/s10856-008-3617-9
IS - 3
Cell Line
Escherichia coli
Fibroblasts
Glass
Ion Exchange
Materials Testing
Silver
Surface Properties
X-Ray Diffraction
Adsorption
Aluminum
Atomic spectroscopy
Bacteriology
Biocompatibility
Cell culture
Ceramic coatings
Doping (additives)
Graphite
Ion exchangers
Leaching
Metal ions
Silicon compounds
Surface treatment
Ternary systems
Testing
aluminum oxide
biomaterial
calcium oxide
glass
immunoglobulin A
immunoglobulin G
silicon dioxide
silver
Addition of al
Anti-bacterial activities
Antibacterial tests
Antimicrobial actions
Atomic absorption spectroscopies
Bacterial colonizations
Bone substitutes
Cytotoxicity tests
Doped samples
Energy dispersions
Glass compositions
Glass systems
Glass-ceramic
Graphite furnaces
In-vitro
In-vitro tests
Inflammatory properties
Ion-exchange process
Ion-exchange treatments
Leaching tests
Material properties
Research activities
Sem
Silver ions
X-ray diffractions
adsorption
article
biocompatibility
bone prosthesis
cell adhesion
cell proliferation
ceramic prosthesis
controlled study
cytotoxicity
drug activity
fibroblast
infection risk
inflammation
ion exchange
leaching
material coating
priority journal
surface doping
synthesis
X ray diffraction
Ion exchange
M3 - Article
PY - 2009
SP - 733-740
ST - Surface silver-doping of biocompatible glass to induce antibacterial
properties. Part I: Massive glass
TI - Surface silver-doping of biocompatible glass to induce antibacterial
properties. Part I: Massive glass
60549110046&doi=10.1007%2fs10856-008-3617-
9&partnerID=40&md5=f4888f64e686996c0119bdf041c85492
VL - 20
ID - 5799
ER -
TY - JOUR
AB - Euphorbia antiquorum was known for its various therapeutic values like anti-
inflammatory, anti-diabetic activity etc. Preliminary phytochemicals screening of
plant extract disclosed the existence of various important bioactive constituents
in hexane, ethanol and aqueous extract of the plant. Aqueous extract of Euphorbia
antiquorum was used for silver and copper nanoparticle synthesis. Synthesized
silver and copper nanoparticle showed the absorbance line at 422 and 367 nm
respectively. Fourier-transform infrared spectroscopy analysis of synthesized
nanoparticles reported the presence of phenol groups, amines and esters. Shape and
morphology of the synthesized nanoparticles were characterized through Field
Emission Scanning Electron Microscopy which showed the spherical shaped
nanoparticles. Energy dispersive X-ray spectroscopy images showed the composition
of analysed nanoparticles. The antioxidant activity of synthesized silver and
copper nanoparticles of Euphorbia antiquorum by hydrogen peroxide scavenging
activity method using ascorbic acid as the standard showed effective scavenging
activity and indicated the potentiality of the plant as a source of natural
antioxidant. Cytotoxicity study of silver nanoparticles showed 73.22% of inhibition
at the concentration of 1000 µg/ml whereas copper nanoparticles at the same
concentration showed 68.85% of inhibition against breast cancer cell line. © 2022
AU - Vijaya Bharathi, S.
AU - Das, M.
DB - Scopus
DO - 10.1016/j.sajb.2022.10.017
KW - Cytotoxicity
Energy dispersive X-ray spectroscopy
Euphorbia antiquorum
Fourier-transform infrared spectroscopy
Nanoparticles
UV–Visible spectrophotometer
angiosperm
cell
electron microscopy
FTIR spectroscopy
inhibition
nanoparticle
phenol
plant extract
spectrophotometry
toxicity
X-ray spectroscopy
M3 - Article
PY - 2022
SP - 410-416
ST - Cytotoxicity effect of nanoparticles of Euphorbia antiquorum on breast cancer
cell line
T2 - South African Journal of Botany
TI - Cytotoxicity effect of nanoparticles of Euphorbia antiquorum on breast cancer
cell line
85140044298&doi=10.1016%2fj.sajb.2022.10.017&partnerID=40&md5=1cb32696fb45d07119977
e864b5b5260
VL - 151
ID - 5005
ER -
TY - JOUR
AB - Zinc and its derivatives requirement increased to enhance human immunity
against the different pandemics, including covid-19. Green synthesis is an emerging
field of research. Zinc oxide (ZnO) nanoparticles have been prepared from
Anoectochilus elatus and characterized using absorption, vibrational and electron
microscope analysis. They were carried for antibacterial, inflammatory control
tendency, and potential antioxidant activities. The brine shrimp lethal assay
tested the biologically derived nanomaterial toxicity and the lethal concentration
(LC50) is 599.79 mu g/ml. The inhibition against the important disease-causing
pathogens was measured against four-gram negative, gram-positive bacteria and two
fungus pathogens. The nanomaterial exposed inhibition zone for gram-positive
bacteria between 17 mm and 25 mm. The inhibition zone against gram-negative
bacteria exists between 19 mm and 24 mm. The anti-inflammatory activity was
assessed by inhibition of protein denaturation and protease inhibitory activity
using nanomaterial. The antioxidant activity was examined using four assays for the
therapeutic activities. The average size range of 60-80 nm nanoparticles has
prepared and exposed the good biological activity between 50 mu g/ml and 100 mu
g/ml. The comparative results of anti-inflammatory and antioxidant assay results
with standards such as Aspirin and vitamin C exposed that two to three times higher
concentrations are required for the fifty percent of inhibitions. The prepared low-
cost nanoparticle has exhibited excellent biological activity without any side
effects and may enhance immunity. (C) 2021 The Author(s). Published by Elsevier
B.V. on behalf of King Saud University.
AN - WOS:000798983400035
AU - Vijayakumar, N.
AU - Bhuvaneshwari, V. K.
AU - Ayyadurai, G. K.
AU - Jayaprakash, R.
AU - Gopinath, K.
AU - Nicoletti, M.
AU - Alarifi, S.
AU - Govindarajan, M.
C6 - MAR 2022
DA - APR
DO - 10.1016/j.sjbs.2021.11.065
IS - 4
PY - 2022
SN - 1319-562X
2213-7106
SP - 2270-2279
ST - Green synthesis of zinc oxide nanoparticles using Anoectochilus elatus, and
their biomedical applications
TI - Green synthesis of zinc oxide nanoparticles using Anoectochilus elatus, and
their biomedical applications
VL - 29
ID - 6326
ER -
TY - JOUR
AB - Kappa-Carrageenan wrapped ZnO nanoparticles (KC-ZnO NPs) was synthesized,
physico-chemically characterized and evaluated their biocompatibility and
antimicrobial therapy against MRSA. XRD showed the highly crystalline and hexagonal
phase structure of ZnO NPs. FETEM confirmed the spherical and hexagonal shaped
particle with the mean size of 97.03 +/- 9.05 nm. The synthesized KC-ZnO NPs
exhibited significant antibacterial activity against MRSA. The biofilm growth of
MRSA was greatly inhibited at 100 mu g/ml as observed through live and dead cell
assay. KC-ZnO NPs have shown invitro anti-inflammatory activity (82%) at 500 mu
g/ml. KC-ZnO NPs was non-toxic to NIH3T3 mouse embryonic fibroblasts cell lines.
Further, no apoptotic and necrotic mediated death in NIH3T3 mouse embryonic
fibroblasts cells were noticed by flow cytometric analysis. KC-ZnO NPs have good
biocompatibility as recorded by the least hemolysis percentage (<3%) up to 100 mu
g/ml, which is much lesser than the acceptable limit. In addition, ecosafety
analysis has shown that KC-ZnO NPs and kappa karrageenan (0-500 mu g/ml) caused no
mortality of A. salina after 48 h. However, bare zinc acetate has shown 35%
mortality of A. salina after 48 h. The results conclude that KC-ZnO NPs could be a
novel antibacterial therapy for the treatment of MRSA associated infectious. (C)
2019 Elsevier B.V. All rights reserved.
AN - WOS:000515200700002
AU - Vijayakumar, S.
AU - Saravanakumar, K.
AU - Malaikozhundan, B.
AU - Divya, M.
AU - Vaseeharan, B.
AU - Duran-Lara, E. F.
AU - Wang, M. H.
DA - FEB 1
DO - 10.1016/j.ijbiomac.2019.12.030
PY - 2020
SN - 0141-8130
1879-0003
SP - 9-18
ST - Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles
for anti MRSA therapy
TI - Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles
for anti MRSA therapy
VL - 144
ID - 6598
ER -
TY - JOUR
AB - Currently, diabetes mellitus (DM) accelerated diabetic foot ulcer (DFU)
remains vivacious health problem related with delayed healing and high amputation
rates which leads to enormous clinical obligation. Keeping in view of the
foregoing, researchers have been made in their efforts to develop novel materials
which accelerate delayed wound healing in the diabetic patient and reduce the
adversative influences of DFUs. The most prominent materials used for the wound
healing application have biocompatibility, low cytotoxicity, excellent
biodegradable properties, and antimicrobial activity properties. Utilization of
nanoparticles has emerged as a protruding scientific and technological revolution
in controlling DFUs. Biopolymers in combination with bioactive nanoparticles having
antimicrobial, antibacterial, and anti-inflammatory properties have great potential
in wound care to enhance the healing process of diabetic wound infectious.
Combination of antibacterial nanoparticles like silver nanoparticles (AgNPs), gold
nanoparticles (AuNPs), copper nanoparticles (CuNPs) etc. with polymeric matrix
could efficiently inhibit bacterial growth and at the same time fastens the healing
process of a wound. This review briefed the recent development of different natural
polymers and antibacterial nanoparticles to mitigate the diabetes mellitus based
DFU. © 2018
AU - Vijayakumar, V.
AU - Samal, S. K.
AU - Mohanty, S.
AU - Nayak, S. K.
DB - Scopus
DO - 10.1016/j.ijbiomac.2018.10.120
KW - Diabetes
Nanoparticle
Wound healing
Animals
Biopolymers
Diabetes Mellitus
Humans
Metal Nanoparticles
Wound Healing
alginic acid
antiinfective agent
cellulose
chitosan
collagen
copper nanoparticle
dextran
elastin
gentamicin
gold nanoparticle
nanoparticle
silver nanoparticle
starch
unclassified drug
yttrium oxide nanoparticle
biopolymer
metal nanoparticle
bacterial growth
carbohydrate metabolism
cerebrovascular accident
circulation
cytotoxicity
diabetic neuropathy
enzyme inhibition
human
immune deficiency
insulin dependent diabetes mellitus
ischemic heart disease
nonhuman
pancreas islet beta cell
pathogenesis
peripheral vascular disease
Review
wound care
wound healing
animal
chemistry
diabetes mellitus
drug effect
pathophysiology
M3 - Review
PY - 2019
SP - 137-148
ST - Recent advancements in biopolymer and metal nanoparticle-based materials in
diabetic wound healing management
TI - Recent advancements in biopolymer and metal nanoparticle-based materials in
diabetic wound healing management
85055679345&doi=10.1016%2fj.ijbiomac.2018.10.120&partnerID=40&md5=d34c4a2df73418c53
50b04ca3185c45c
VL - 122
ID - 5397
ER -
TY - JOUR
AB - During the last few decades, gold nanoparticles (AuNP's) have gained
considerable attention in nanomedicine and expanded its application in clinical
diagnosis and as therapeutics. Employing plant extract for synthesising gold
nanoparticles proves to be an eco-friendly technology for large scale production.
It is highly economical and suitable for biological applications by negating the
use of chemicals involved in conventional route. In this study, AuNP's was prepared
by a simple one step method of employing aqueous Mangifera indica seed extract as a
reducing agent. Scanning electron microscopy and transmission electron microscopy
revealed spherical shaped nanoparticles and dynamic light scattering analysis
indicated the AuNP's to be approximately 46.8 nm in size. AuNP's efficiently
inhibited the growth of E. coll. and S. aureus by its inherent ability to generate
reactive oxygen species (ROS) and exhibited detrimental effects towards the tested
bacterial species. Biocompatibility assessment indicated the non-toxic nature of
AuNP's towards mesenchymal stem cells at 25 mu g/ml and interestingly, suppressed
the growth of human gastric cancer cells under in vitro culture conditions. AuNP's
significantly exhibited anti-angiogenic property in chick chorioallantoic membrane
model (CAM) by downregulating Ang-1/Tie2 pathway. Overall, the synthesized AuNP's
exhibited antibacterial and anti-angiogenic properties with high biocompatibility
thereby supporting its candidature for various biomedical applications. It can be
employed in suppressing tumor growth, combat inflammatory diseases that necessitate
the involvement of angiogenesis suppression, and antibacterial activity is suitable
for its clinical translation to negate surgery associated infections.
AN - WOS:000438312600049
AU - Vimalraj, S.
AU - Ashokkumar, T.
AU - Saravanan, S.
DA - SEP
DO - 10.1016/j.biopha.2018.05.151
PY - 2018
SN - 0753-3322
1950-6007
SP - 440-448
ST - Biogenic gold nanoparticles synthesis mediated by Mangifera indica seed
aqueous extracts exhibits antibacterial, anticancer and anti-angiogenic properties
TI - Biogenic gold nanoparticles synthesis mediated by Mangifera indica seed
aqueous extracts exhibits antibacterial, anticancer and anti-angiogenic properties
VL - 105
ID - 6785
ER -
TY - JOUR
AB - Titanium dioxide nanoparticle (TNP) has been suggested for use in fish farms
to prevent or alleviate bacterial diseases owing to its bactericidal property.
Unfortunately, the interaction of TNP with cells impaired the host defenses of fish
resulting in increased mortality during bacterial challenges. The present study
evaluated the efficacy of the ethanolic extract of Tinospora cordifolia (TCE) as a
dietary supplement in ameliorating TNP induced toxicity in Nile tilapia
(Oreochromis niloticus). The fishes were exposed to environmentally relevant
concentration (10 mg/L) of TNP for 14 days and the effect of TCE supplemented feed
at 3 different doses (5, 10, and 15 g/kg) was studied. TCE signally increased the
weight gain, specific growth rate, and decreased feed conversion ratio in fish. TCE
significantly (P < 0.05) ameliorated the toxic effects caused by TNP by increasing
the antioxidant (CAT, SOD, GPx) activity and decreasing the levels of serum enzymes
(ALT, AST, ALP, ACP), macromolecular oxidation, excessive ROS production, and pro-
inflammatory cytokines (IL-1 beta, IL-6, IL-8, INF-gamma, TNF-alpha, PGE-2). TNP
bioaccumulation and histopathological alterations in gill, liver, and kidney were
also significantly alleviated by TCE supplementation. TCE perceptibly regulated the
expression of heat shock proteins (HSP60, -70), MAPKs (pERK1/2, pp38), antioxidant
(NRF2, Keap1, HO-1), apoptotic (p53, PDRG1), and antiapoptotic (AKT, Bcl2) proteins
in fish. Regarding disease resistance, the TCE co-treated groups showed reduced
cumulative mortality and higher relative percent survival with A. hydrophila. Our
results suggest that TNPinduced apoptosis is mediated by the MAPK/NRF2/Keap1
pathway and underlines the therapeutic potential of TCE in aqua-farming.
AN - WOS:000604566700013
AU - Vineetha, V. P.
AU - Devika, P.
AU - Prasitha, K.
AU - Anilkumar, T. V.
C7 - 108908
DA - FEB
DO - 10.1016/j.cbpc.2020.108908
PY - 2021
SN - 1532-0456
1878-1659
ST - Tinospora cordifolia ameliorated titanium dioxide nanoparticle-induced
toxicity via regulating oxidative stress-activated MAPK and NRF2/Keap1 signaling
pathways in Nile tilapia (Oreochromis niloticus)
T2 - COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
TI - Tinospora cordifolia ameliorated titanium dioxide nanoparticle-induced
toxicity via regulating oxidative stress-activated MAPK and NRF2/Keap1 signaling
pathways in Nile tilapia (Oreochromis niloticus)
VL - 240
ID - 6590
ER -
TY - JOUR
AB - Background: Syzygium aromaticum, also known as clove, and its essential oil
has already been proved to have antioxidant, anti-inflammatory and anticancer
properties. Clove is used in various foods owing to its potent antimicrobial and
antioxidant properties. Essential oil extractedfrom clove has been used in
traditional medicine for treating various ailments. Methods: In silico analyses of
phytocompounds of Syzygium aromaticum namely eugenol, B-caryophyllene, gallic acid,
crategolic acid, kaempferol, quercetin, cinnamaldehyde, and oleanolic acid were
docked with three apoptotic proteins involved in breast cancer, namely BCL-2, BAX
and APAF-1 using AUTODOCK. In addition, flower bud extract of Syzygiumaromaticumwas
used for the synthesis of AgNPs (silver nanoparticles). The synthesized clove-
silver nanoparticles were then characterized using various techniques such as
Ultraviolet-visible spectrophotometry, FTIR, FESEM-EDX, DLS and zeta potential to
determine the particle size, shape, crystalline structure, and stability of CL-
AgNPs and tested for its anticancer potential in MCF-7 cell lines. Results: In
silico analysis predicted that phytochemicals of clove have good interactions with
the apoptosis related proteins of breast cancer. In vitro assay confirmed the
cytotoxic effect of the synthesized CL-AgNPs on breast cancer cells using the MCF-7
cell line with the IC50 value of 58.64 µg/ml. Conclusion: In vitro analysis of the
anticancer activity of CL-AgNPs in MCF-7 cell line supports the in silico study by
proving active interactions between the phytochemicals of clove and target proteins
of the breast cancer and hence Syzygium aromaticum has been proved to possess
potential anticancer property. Further research is needed to consider clove-silver
nanoparticles as a novel drug for treating breast cancer. © 2023 Universidad de los
Andes, Bogota Colombia. All rights reserved.
AU - Vishal, J.
AU - Ranjani, S.
AU - Karunya, R. J.
AU - Hemalatha, S.
DB - Scopus
DO - 10.32768/abc.2023103291-300
IS - 3
KW - Anticancer agent
Antioxidant
Breast cancer
Metallic nanoparticle
Molecular docking
Syzygium
M3 - Article
PY - 2023
SP - 291-300
ST - Synthesis, Characterization and Evaluation of Antioxidant, Anticancer and
Toxicity Properties of Silver Nanoparticles Synthesized From Syzygium Aromaticum
T2 - Archives of Breast Cancer
TI - Synthesis, Characterization and Evaluation of Antioxidant, Anticancer and
Toxicity Properties of Silver Nanoparticles Synthesized From Syzygium Aromaticum
85168091774&doi=10.32768%2fabc.2023103291-
300&partnerID=40&md5=6e9ad4c64b453ec1d95d72baaf1b5af5
VL - 10
ID - 4959
ER -
TY - JOUR
AB - 1. Sulindac, cis-5-fluoro-2-methyl-1-(p-methylsulphinylbenzylidene)indene-3-
acetic acid, inhibits growth of colon polyps and cancers. This effect has been
attributed to inhibition of prostaglandin synthesis but more recent observations
indicate that, in vitro, cells that do not have cyclo-oxygenase nor RNA for
synthesis of such enzymes are affected by sulindac. Therefore the presumptive
effect is probably not correct. 2. It has also been found that sulindac stimulates
apoptosis. It is herein postulated that in tumour cells such effects may be due to
interaction of the anionic form of the drug with protons in the intermembrane space
of mitochondria to disrupt the potential across the inner mitochondrial membrane
and thereby initiate apoptosis. Normal cells are not affected.
AN - WOS:000076149800020
AU - Waddell, W. R.
DA - SEP
DO - 10.1042/CS19970251
IS - 3
PY - 1998
SN - 0143-5221
SP - 385-388
ST - Stimulation of apoptosis by sulindac and piroxicam
T2 - CLINICAL SCIENCE
TI - Stimulation of apoptosis by sulindac and piroxicam
VL - 95
ID - 6370
ER -
TY - JOUR
AB - Bacteria can easily adhere on the surface of implants and subsequently form
biofilm which lead to serious critical issues in the application. In the current
work, poly(acrylic acid) (PAA) was firstly modified with dopamine (dopa) into dopa-
PAA and was self-assembled with poly(ethylenimine)-AgNO3 (PEI-AgNO3) into
multilayer films via layer-by-layer self-assembly technique. AgNO3 were in situ
reduced into silver nano particles (Ag NPs) in the process of multilayer film self-
assembling using bio-inspired dopa as reducing agent. The morphology of the Ag NPs
was characterized using transmission electron microscopy. In vitro, the
(dopa-PAA/PEI-Ag NPs)(n) multilayer films showed a long-term sustained release of
Ag+ in PBS buffer. Zone of inhibition assays and the bacterial LIVE/DEAD staining
methods demonstrated the high efficient and long-term bactericidal properties of
the multilayer films. For the in vivo subcutaneous antibacterial tests in New
Zealand white rabbits, the wound appearance and the histopathology analysis showed
that implantation of the antibacterial coating modified PDMS promoted wound healing
and showed good anti-inflammatory effect. The multilayer films also proved to be
low cytotoxicity towards human lens epithelial cells, which can potentially be
widely used to modify biomedical implants. (C) 2017 Elsevier Ltd. All rights
reserved.
AN - WOS:000411537900021
AU - Wang, B. L.
AU - Ye, Z.
AU - Xu, Q. W.
AU - Sun, L.
AU - Wang, Y. Q.
AU - Shi, S.
AU - Wang, Z. F.
AU - Xu, X.
AU - Qu, J.
AU - Nan, K. H.
DA - SEP
DO - 10.1016/j.polymertesting.2017.06.023
PY - 2017
SN - 0142-9418
1873-2348
SP - 162-170
ST - In situ construction of Ag NPs in bio-inspired multilayer films for long-term
bactericidal and biofilm inhibition properties
T2 - POLYMER TESTING
TI - In situ construction of Ag NPs in bio-inspired multilayer films for long-term
VL - 62
ID - 6455
ER -
TY - JOUR
AB - Bacteria can easily adhere on the surface of implants and subsequently form
biofilm which lead to serious critical issues in the application. In the current
work, poly(acrylic acid) (PAA) was firstly modified with dopamine (dopa) into dopa-
PAA and was self-assembled with poly(ethylenimine)-AgNO3 (PEI-AgNO3) into
multilayer films via layer-by-layer self-assembly technique. AgNO3 were in situ
reduced into silver nano particles (Ag NPs) in the process of multilayer film self-
assembling using bio-inspired dopa as reducing agent. The morphology of the Ag NPs
was characterized using transmission electron microscopy. In vitro, the
(dopa-PAA/PEI-Ag NPs)n multilayer films showed a long-term sustained release of Ag+
in PBS buffer. Zone of inhibition assays and the bacterial LIVE/DEAD staining
methods demonstrated the high efficient and long-term bactericidal properties of
the multilayer films. For the in vivo subcutaneous antibacterial tests in New
Zealand white rabbits, the wound appearance and the histopathology analysis showed
that implantation of the antibacterial coating modified PDMS promoted wound healing
and showed good anti-inflammatory effect. The multilayer films also proved to be
low cytotoxicity towards human lens epithelial cells, which can potentially be
widely used to modify biomedical implants. © 2017
AU - Wang, B.
AU - Ye, Z.
AU - Xu, Q.
AU - Sun, L.
AU - Wang, Y.
AU - Shi, S.
AU - Wang, Z.
AU - Xu, X.
AU - Qu, J.
AU - Nan, K.
DB - Scopus
DO - 10.1016/j.polymertesting.2017.06.023
KW - Antibacterial
Bio-inspired
In situ reduction
Multilayer films
Nano silver
Amines
Biofilms
Biomimetics
Morphology
Multilayers
Nanoparticles
Silver
Silver compounds
Tissue regeneration
Bactericidal properties
Layer-by-layer self assembly techniques
New Zealand White rabbit
Situ reduction
M3 - Article
PY - 2017
SP - 162-170
ST - In situ construction of Ag NPs in bio-inspired multilayer films for long-term
T2 - Polymer Testing
TI - In situ construction of Ag NPs in bio-inspired multilayer films for long-term
85021724036&doi=10.1016%2fj.polymertesting.2017.06.023&partnerID=40&md5=80f815555b5
0cd201c4628166a5d3499
VL - 62
ID - 5521
ER -
TY - JOUR
AB - Regarding applicative, facile, green chemical research, a bio-inspired
approach is being reported for the synthesis of Au nanoparticles by pectin (PEC) as
a natural reducing and stabilizing agent without using any toxic and harmful
reagent under ultrasonic condition. The biosynthesized Au NPs@PEC were
characterized by advanced physicochemical techniques like ultraviolet-visible (UV-
Vis), Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron
Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray
spectroscopy (EDX), and X-ray Diffraction (XRD) study. It has been established that
pectin-stabilized Au nanoparticles have a spherical shape with a mean diameter from
5 to 10 nm. In the medicinal part of the present research, the lung BEAS-2B, WI-38,
CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell viability was determined by trypan blue
assay. The caspase activity colorimetric assay kit and Rhodamine123 fluorescence
dye were used to determine the caspase-3 activity and mitochondrial membrane
potential, respectively. Apoptosis and DNA fragmentation were determined by the
TUNEL test. Also, the inflammatory cytokines concentrations were evaluated by the
Rat inflammatory cytokine assay kit. Au NPs@PEC-treated cell cutlers decreased
significantly (p < 0.01) the caspase-3 activity, inflammatory cytokines
concentrations, and DNA fragmentation, and enhanced the mitochondrial membrane
potential and cell viability in the high concentration of Methotrexate-treated lung
BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cells. In the antioxidant
test, the IC50 of Au NPs@PEC nanocomposite and BHT against DPPH free radicals were
203 and 181 mg/mL, respectively. Finally, Au NPs@PEC may be used as a pulmonary
protective supplement to treat acute lung injury. (c) 2021 The Authors. Published
by Elsevier B.V. on behalf of King Saud University. This is an open access article
under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
AN - WOS:000734882600003
AU - Wang, D. X.
AU - Pu, J.
AU - Liao, Y. B.
AU - Liu, J. H.
AU - Hu, G.
C6 - NOV 2021
C7 - 103533
DA - JAN
DO - 10.1016/j.arabjc.2021.103533
IS - 1
PY - 2022
SN - 1878-5352
1878-5379
ST - Pulmonary protective effects of ultrasonic green synthesis of gold
nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung
BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
TI - Pulmonary protective effects of ultrasonic green synthesis of gold
nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung
BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
VL - 15
ID - 6574
ER -
TY - JOUR
AB - Long silver nanowires (AgNWs, >5 mu m) have shown promising applications in
next generation biomaterials. However, the toxicity of long AgNWs is not well
characterized in terms of their size. In this study, five AgNWs types, including
SAgNW30 (length: 5-10 mu m; diameter: 30 nm), MAgNW30 (length: 20-30 mu m;
diameter: 30 nm), LAgNW30 (length: similar to 100 pm; diameter: 30 nm), LAgNW50
(length: similar to 100 gm; diameter: 50 nm), and LAgNW100 (length: similar to 100
gm; diameter: 100 nm), were used to investigate the size-dependent phagocytosis and
cytotoxicity in macrophage. It showed that SAgNW30, MAgNW30, LAgNW30 can be fully
phagocytosed by macrophages, but LAgNW50 and LAgNW100 frustrated the phagocytosis.
It demonstrated that LAgNW30 can be internalized into macrophage in a curly manner.
The size-dependent cytotoxicity was observed in cell viability, apoptosis,
mitochondrial damage, phenotypic transition, and inflammatory response in AgNWs-
treated macrophage. The AgNWs-induced cytotoxicity was depended on their length and
diameter, increased gradually in the order of SAgNW30 > MAgNW30 > LAgNW30 > LAgNW50
> LAgNW100. The findings presented here will assist in the evaluation of the size-
dependent cytotoxicity mediated by long AgNWs. (C) 2019 Elsevier Ltd. All rights
reserved.
AN - WOS:000496896700067
AU - Wang, F. B.
AU - Wang, Y. Y.
AU - Yao, X. L.
AU - Ma, C. Y.
AU - Yin, Y. G.
AU - Song, M. Y.
C7 - 124565
DA - DEC
DO - 10.1016/j.chemosphere.2019.124565
PY - 2019
SN - 0045-6535
1879-1298
ST - Length and diameter-dependent phagocytosis and cytotoxicity of long silver
nanowires in macrophages
T2 - CHEMOSPHERE
TI - Length and diameter-dependent phagocytosis and cytotoxicity of long silver
VL - 237
ID - 6128
ER -
TY - JOUR
AB - Long silver nanowires (AgNWs, >5 μm) have shown promising applications in
next generation biomaterials. However, the toxicity of long AgNWs is not well
characterized in terms of their size. In this study, five AgNWs types, including
SAgNW30 (length: 5–10 μm; diameter: 30 nm), MAgNW30 (length: 20–30 μm; diameter: 30
nm), LAgNW30 (length: ∼100 μm; diameter: 30 nm), LAgNW50 (length: ∼100 μm;
diameter: 50 nm), and LAgNW100 (length: ∼100 μm; diameter: 100 nm), were used to
investigate the size-dependent phagocytosis and cytotoxicity in macrophage. It
showed that SAgNW30, MAgNW30, LAgNW30 can be fully phagocytosed by macrophages, but
LAgNW50 and LAgNW100 frustrated the phagocytosis. It demonstrated that LAgNW30 can
be internalized into macrophage in a curly manner. The size-dependent cytotoxicity
was observed in cell viability, apoptosis, mitochondrial damage, phenotypic
transition, and inflammatory response in AgNWs-treated macrophage. The AgNWs-
induced cytotoxicity was depended on their length and diameter, increased gradually
in the order of SAgNW30 > MAgNW30 > LAgNW30 > LAgNW50 > LAgNW100. The findings
presented here will assist in the evaluation of the size-dependent cytotoxicity
mediated by long AgNWs. © 2019 Elsevier Ltd
AU - Wang, F.
AU - Wang, Y.
AU - Yao, X.
AU - Ma, C.
AU - Yin, Y.
AU - Song, M.
C7 - 124565
DB - Scopus
DO - 10.1016/j.chemosphere.2019.124565
KW - Cytotoxicity
Long AgNWs
Macrophage
Phagocytosis
Size-dependent
Animals
Cell Survival
Macrophages
Mice
Nanowires
RAW 264.7 Cells
Silver
Antigen-antibody reactions
Cell death
nanowire
silver nanoparticle
silver
Cell viability
Diameter dependent
Mitochondrial damage
Silver nanowires
Size dependent
apoptosis
cell component
phenotype
toxicity
animal cell
Article
cell size
cell viability
controlled study
cytokine production
cytotoxicity
dispersion
inflammation
internalization
macrophage
mouse
nanotoxicology
nonhuman
particle size
phagocytosis
animal
cell survival
chemistry
drug effect
pathology
RAW 264.7 cell line
M3 - Article
PY - 2019
ST - Length and diameter-dependent phagocytosis and cytotoxicity of long silver
T2 - Chemosphere
TI - Length and diameter-dependent phagocytosis and cytotoxicity of long silver
85070535878&doi=10.1016%2fj.chemosphere.2019.124565&partnerID=40&md5=6a504e7091cb48
1a78c79a94bcd4c6d6
VL - 237
ID - 5412
ER -
TY - JOUR
AB - Background: 5-demethylnobiletin is a natural polymethoxyflavone which is
isolated from the extract of citrus fruits peels. It exhibits a broad spectrum of
biological activities such as anti-cancer, anti-inflammatory, cardiovascular
protective and neuroprotective effects, however, its effect in melanogenesis
remains uninvestigated. Purpose: Melanin synthesis is a very important biological
process in curing disease such as vitiligo with depigmentation on the skin. In the
current work, we aim to confirm the bioactivity and mechanism of 5-
demethylnobiletin in stimulating melanogenesis. Study Design: To confirm the
mechanistic role of 5-demethylnobiletin in enhancing melanogenesis, its effect on
the activity of tyrosinase, together with the level of microphthalmia-associated
transcription factor (MITF), Trp-1, Trp-2, melanocyte-specific marker protein
PMEL17, Rab27a, Melanophilin and Myosin VA were studied in B16F10 melanoma cells.
Methods: Multiple biological assays on melanogenesis-associated proteins such as
melanin content detection, tyrosinase activity colorimetric assay, qPCR, western
blot analysis, dual-luciferase reporter assay, cAMP activity assay and Fontana-
Masson ammoniacal silver staining were used to confirm the role of 5-
demethylnobiletin in stimulating melanin synthesis and the transportation of
melanosomes. Results: As confirmed by multiple biological assays, 5-
demethylnobiletin is found to stimulate dendrite structure formation in cells,
melanin synthesis and the transportation of melanosomes, via inducing the
phosphorylation of cAMP response element-binding protein (CREB) and increasing the
intracellular levels of cAMP in vitro through the PKA-dependent pathway.
Conclusion: The findings suggested that 5-demethylnobiletin may be considered as a
potential natural product candidate for patients with pigment disorder. © 2022
Elsevier GmbH
AU - Wang, H. M.
AU - Qu, L. Q.
AU - Ng, J. P. L.
AU - Zeng, W.
AU - Yu, L.
AU - Song, L. L.
AU - Wong, V. K. W.
AU - Xia, C. L.
AU - Law, B. Y. K.
C7 - 153941
DB - Scopus
DO - 10.1016/j.phymed.2022.153941
KW - 5-demethylnobiletin
B16F10
cAMP/CREB pathway
Melanogenesis
5 demethylnobiletin
cyclic AMP
cyclic AMP responsive element binding protein
dopachrome tautomerase
flavanone
melanin
melanocyte protein Pmel 17
microphthalmia associated transcription factor
monophenol monooxygenase
myosin Va
Ras related protein Rab 27A
tyrosinase related protein 1
unclassified drug
animal cell
Article
B16-F10 cell line
bioassay
biological activity
cell activation
cell viability
Citrus
colorimetry
controlled study
dendrite
drug cytotoxicity
drug effect
drug mechanism
drug purity
drug structure
enzyme activity
in vitro study
luciferase assay
Masson staining
melanogenesis
melanosome
mouse
nonhuman
pigmentation
protein expression
signal transduction
silver staining
upregulation
Western blotting
M3 - Article
PY - 2022
ST - Natural Citrus flavanone 5-demethylnobiletin stimulates melanogenesis through
the activation of cAMP/CREB pathway in B16F10 cells
T2 - Phytomedicine
TI - Natural Citrus flavanone 5-demethylnobiletin stimulates melanogenesis through
the activation of cAMP/CREB pathway in B16F10 cells
85123685096&doi=10.1016%2fj.phymed.2022.153941&partnerID=40&md5=b9c80718f17066f9e5e
96ddec552b608
VL - 98
ID - 5113
ER -
TY - JOUR
AB - Periodontitis noted as dental care disease, which occur the inflammatory
process in the teeth tissues surrounding which has the response to bacterial
accumulation. The main objectives of the present investigations were to improve the
multifunctional nanocomposite with PLGA@fluorapatite and enhance the bactericidal
inhibitory efficiency against periodontitis-related pathogens. The peptide modified
fluorapatite@PLGA nanocomposite were synthesized and physico-chemically
characterized. The observed analytical results demonstrated rod-like nanoparticles
formation with favorable human cell compatibility and toxicity against bacterial
pathogens. In addition, the use of FA and PLGA dual agents in the composite
achieved the greatest reduction in the biofilm growth and the metabolic activity of
polysaccharide production. The CFU count in the biofilms was reduced by nearly 3
orders for periodontal pathogens. Therefore, the new modified peptide PLGA-FA
composite promising in the root caries restorations to inhibit periodontitis
related pathogens.
AN - WOS:000655267800001
AU - Wang, J. H.
AU - Li, Y.
AU - Jing, J.
AU - Yue, H. L.
AU - Zhang, L. L.
AU - Hua, W.
AU - Li, N.
AU - Liu, X.
AU - Han, J. A.
C7 - 055013
DA - MAY
DO - 10.1088/2053-1591/abf2e8
IS - 5
PY - 2021
SN - 2053-1591
ST - Practical evaluations of bioactive peptide-modified Fluorapatite/PLGA
multifunctional nano-clustery composite against for root caries restorations to
inhibit periodontitis-related pathogens in periodontitis care
TI - Practical evaluations of bioactive peptide-modified Fluorapatite/PLGA
multifunctional nano-clustery composite against for root caries restorations to
inhibit periodontitis-related pathogens in periodontitis care
VL - 8
ID - 6637
ER -
TY - JOUR
AB - The primary idea behind this study is to synthesize Saudi Origanum vulgare L.
extract mediated AuNPs and to investigate their efficacy in significantly treating
AMD. The biocompatible AuNPs were tested for their radical scavenging efficiency in
vitro. Also, The effects of AuNPs in suppressing the angiogenic protein expression
and inflammatory cytokine levels were analyzed in ARPE-19 (human retinal pigment
epithelium-19) as well as and human umbilical vein endothelium cell lines in vitro.
From the ORAC findings, it was evident that the bio synthesized AuNPs have
presented momentous anti-oxidative activity in reducing the apoptosis in H2O2
treated ARPE-19 cell lines. In addition, the biocompatible AuNPs exhibited
significant potential in suppressing the LPS stimulated inflammatory reaction in
ARPE-19 cell lines while demonstrating inhibitory expression of growth factor in
ARPE-19 as well as in umbilical vein endothelium cell lines. The experimental
findings in this study have suggested that the synthesized AuNPs are biocompatible
with significant potential in defending against angiogenesis, apoptosis, and
generation of pro-inflammatory cytokines in AMD disease.
AN - WOS:000518386500033
AU - Wang, J.
AU - Wang, Y.
AU - Li, Q. M.
C7 - 101377
DA - FEB
DO - 10.1016/j.jddst.2019.101377
PY - 2020
SN - 1773-2247
ST - Synthesis of AuNPs using plant polyphenols and their potential treatment for
age-related macular degeneration
TI - Synthesis of AuNPs using plant polyphenols and their potential treatment for
age-related macular degeneration
VL - 55
ID - 6662
ER -
TY - JOUR
AB - Purpose: To evaluate the adverse vascular effects of nanoparticles (NPs) in
vitro, extensive studies have investigated the toxicity of NPs on endothelial
cells, but the knowledge of potential toxicity on human smooth-muscle cells (SMCs)
is currently limited. Methods: This study compared the toxicity of TiO 2 , ZnO, and
Ag NPs to human aortic SMCs. Results: Only ZnO NPs significantly induced
cytotoxicity, accompanied by increased intracellular reactive oxygen species, Zn
ions, and endoplasmic reticulum stress biomarkers (DDIT3 expression and p-Chop
proteins). All the NPs significantly promoted the release of soluble VCAM1 and
soluble sICAM1, but not IL6, which suggested that metal-based NPs might promote
inflammatory responses. Furthermore, KLF4 expression (a transcription factor for
SMC-phenotype switch) was significantly induced by TiO 2 NPs and modestly by ZnO
NPs, but the expression of CD68 remained unaltered. Conclusion: Our data indicated
that ZnO NPs were more cytotoxic to human aortic SMCs than TiO 2 and Ag NPs at the
same mass concentrations, which might have been associated with intracellular
reactive oxygen species, Zn ions, and endoplasmic reticulum stress. © 2018 Wang et
al.
AU - Wang, M.
AU - Yang, Q.
AU - Long, J.
AU - Ding, Y.
AU - Zou, X.
AU - Liao, G.
AU - Cao, Y.
DB - Scopus
DO - 10.2147/IJN.S188175
KW - Cytotoxicity
Endoplasmic reticulum stress
ER stress
HASMCs
Human aortic smooth-muscle cells
Inflammation
Metal-based nanoparticles
NPs
Animals
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Aorta
Biomarkers
Cell Death
Cell Survival
Cytokines
Endocytosis
Humans
Ions
Kruppel-Like Transcription Factors
Metal Nanoparticles
Models, Biological
Myocytes, Smooth Muscle
Silver
Titanium
Zinc Oxide
biological marker
CD68 antigen
kruppel like factor 4
silver nanoparticle
autacoid
CD68 antigen, human
cytokine
differentiation antigen
ion
kruppel like factor
leukocyte antigen
metal nanoparticle
silver
titanium
titanium dioxide
zinc oxide
antigen expression
aortic smooth muscle cell
Article
comparative study
controlled study
cytotoxicity
gene switching
human
human cell
in vitro study
inflammation
nanotoxicology
protein expression
animal
aorta
biological model
cell death
cell survival
cytology
drug effect
endocytosis
metabolism
smooth muscle cell
ultrastructure
M3 - Article
PY - 2018
SP - 8037-8049
ST - A comparative study of toxicity of TiO 2 , ZnO, and Ag nanoparticles to human
aortic smooth-muscle cells
TI - A comparative study of toxicity of TiO 2 , ZnO, and Ag nanoparticles to human
aortic smooth-muscle cells
85058820081&doi=10.2147%2fIJN.S188175&partnerID=40&md5=a5d346c29356f5d025bf6d1540f3
bbc3
VL - 13
ID - 5497
ER -
TY - JOUR
AB - Microbial infection accounts for many dental diseases and treatment failure.
Therefore, the antibacterial properties of dental biomaterials are of great
importance to the long-term results of treatment. Silver-based biomaterials (AgBMs)
have been widely researched as antimicrobial materials with high efficiency and
relatively low toxicity. AgBMs have a broad spectrum of antimicrobial properties,
including penetration of microbial cell membranes, damage to genetic material,
contact killing, and dysfunction of bacterial proteins and enzymes. In particular,
advances in nanotechnology have improved the application value of AgBMs. Hence, in
many subspecialties of dentistry, AgBMs have been researched and employed, such as
caries arresting or prevention, root canal sterilization, periodontal plaque
inhibition, additives in dentures, coating of implants and anti-inflammatory
material in oral and maxillofacial surgery. This paper aims to provide an overview
of the application approaches of AgBMs in dentistry and present better guidance for
oral antimicrobial therapy via the development of AgBMs.
AN - WOS:000750006600001
AU - Wang, Q. Y.
AU - Zhang, Y.
AU - Li, Q.
AU - Chen, L.
AU - Liu, H.
AU - Ding, M.
AU - Dong, H.
AU - Mou, Y. B.
DO - 10.2147/IJN.S349238
PY - 2022
SN - 1178-2013
SP - 443-462
ST - Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in
Dentistry
TI - Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in
Dentistry
VL - 17
ID - 6307
ER -
TY - JOUR
AB - The current study aimed to explore the effects of supplementing paraformic
acid (PFA) into broilers’ diet on growth performance, inflammatory responses, and
liver protection. A total of 567 healthy one-day-old broilers were used in a 42-d
study, and they were randomized into three groups. Broilers were fed a basal diet
(CON group) or the basal diet supplemented with either 50 mg/kg aureomycin (AB
group) or 1000 mg/kg PFA (PFA group). The results showed that the PFA and AB groups
had a higher feed conversion rate than the CON group from day 21 to 42 (p < 0.05).
Dietary PFA or aureomycin supplementation decreased serum levels of interleukin
(IL)-1β, IL-6, IL-10, alanine transaminase, diamine oxidase, and D-lactate, and
significantly increased serum concentrations of immunoglobulin (Ig) A, IgM, and
complement C4 (p < 0.05). Moreover, dietary PFA or aureomycin supplementation
decreased hepatic levels of caspase-1, NOD-like receptor family pyrin domain
containing 3 (NLRP3), tumor necrosis factor-alpha, IL-6, and IL-18, as well as NF-
κB mRNA expression (p < 0.05). Above all, PFA supplementation into the broilers’
diet improved growth performance, inhibited inflammatory responses, and benefited
liver protection. The protective effects of PFA on the liver might be related to
inhibition of caspase-1-induced pyroptosis via inactivating the NF-κB/NLRP3
inflammasome axis in broiler chickens. © 2022 by the authors.
AU - Wang, Q.
AU - Niu, J.
AU - Liu, Y.
AU - Jiao, N.
AU - Huang, L.
AU - Jiang, S.
AU - Yan, L.
AU - Yang, W.
AU - Li, Y.
C7 - 2825
DB - Scopus
DO - 10.3390/ani12202825
IS - 20
KW - antibiotic growth promoter
chicken
feed additives
formic acid
liver
cadmium
microplastic
nanoplastic
polystyrene
silver nitrate
trypan blue
animal experiment
Article
blood cell
cell viability
comet assay
controlled study
cytotoxicity
DNA damage
Drosophila melanogaster
genetic damage
genotoxicity
genotoxicity assay
health hazard
hemolymph
intestine cell
intestine injury
larva
nonhuman
oxidative stress
pollutant
stereomicroscopy
toxicity testing
trypan blue assay
M3 - Article
PY - 2022
ST - Supplementation of Paraformic Acid as a Substitute for Antibiotics in the
Diet Improves Growth Performance and Liver Health in Broiler Chickens
T2 - Animals
TI - Supplementation of Paraformic Acid as a Substitute for Antibiotics in the
Diet Improves Growth Performance and Liver Health in Broiler Chickens
85140398812&doi=10.3390%2fani12202825&partnerID=40&md5=ac49ac30d6d01876e6e61f2594c8
ba17
VL - 12
ID - 5118
ER -
TY - JOUR
AB - Microbial infection accounts for many dental diseases and treatment failure.
Therefore, the antibacterial properties of dental biomaterials are of great
importance to the long-term results of treatment. Silver-based biomaterials (AgBMs)
have been widely researched as antimicrobial materials with high efficiency and
relatively low toxicity. AgBMs have a broad spectrum of antimicrobial properties,
including penetration of microbial cell membranes, damage to genetic material,
contact killing, and dysfunction of bacterial proteins and enzymes. In particular,
advances in nanotechnology have improved the application value of AgBMs. Hence, in
many subspecialties of dentistry, AgBMs have been researched and employed, such as
caries arresting or prevention, root canal sterilization, periodontal plaque
inhibition, additives in dentures, coating of implants and anti-inflammatory
material in oral and maxillofacial surgery. This paper aims to provide an overview
of the application approaches of AgBMs in dentistry and present better guidance for
oral antimicrobial therapy via the development of AgBMs. © 2022, Dove Medical Press
Ltd. All rights reserved.
AU - Wang, Q.
AU - Zhang, Y.
AU - Li, Q.
AU - Chen, L.
AU - Liu, H.
AU - Ding, M.
AU - Dong, H.
AU - Mou, Y.
DB - Scopus
DO - 10.2147/IJN.S349238
KW - Antimicrobial
Biomaterials
Dentistry
Nanotechnology
Oral biofilm formation
Silver
antiinfective agent
biomaterial
chlorhexidine
dental material
edetic acid
gutta percha
hypochlorite sodium
silver
silver diamine fluoride
unclassified drug
antimicrobial therapy
biofilm
biosafety
biosecurity
dental caries
dentistry
human
maxillofacial surgery
nanotechnology
nonhuman
oral surgery
periodontics
prosthodontics
Pseudomonas stutzeri
Review
tooth filling
M3 - Review
PY - 2022
SP - 443-462
ST - Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in
Dentistry
TI - Therapeutic Applications of Antimicrobial Silver-Based Biomaterials in
Dentistry
85123972549&doi=10.2147%2fIJN.S349238&partnerID=40&md5=f114a49af0141a7466170748658c
b167
VL - 17
ID - 5154
ER -
TY - JOUR
AB - Co ions released due to corrosion of Co nanoparticles (CoNPs) in the
lysosomes of macrophages may be a factor in the particle-induced cytotoxicity and
aseptic inflammation accompanying metal-on-metal (MOM) hip prosthesis failure.
Here, we show that CoNPs are easily dissolved under a low pH, simulating the acidic
lysosomal environment. We then used bafilomycin A1 to change the pH inside the
lysosome to inhibit intracellular corrosion of CoNPs and then investigated its
protective effects against CoNP-induced cytotoxicity and aseptic inflammation on
murine macrophage RAW264.7 cells. XTT {2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-
[(phenylamino) carbonyl]-2H-tetrazolium hydroxide} assays revealed that bafilomycin
A1 can significantly decrease CoNP-induced cytotoxicity in RAW264.7 cells. Enzyme-
linked immunosorbent assays showed that bafilomycin A1 can significantly decrease
the subtoxic concentration of CoNP-induced levels of pro-inflammatory cytokines
(tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6), but has no
effect on anti-inflammatory cytokines (transforming growth factor-beta and
interleukin-10) in RAW264.7 cells. We studied the protective mechanism of
bafilomycin A1 against CoNP-induced effects in RAW264.7 cells by measuring
glutathione/oxidized glutathione (GSH/GSSG), superoxide dismutase, catalase, and
glutathione peroxidase levels and employed scanning electron microscopy,
transmission electron microscopy, and energy dispersive spectrometer assays to
observe the ultrastructural cellular changes. The changes associated with apoptosis
were assessed by examining the pAKT and cleaved caspase-3 levels using Western
blotting. These data strongly suggested that bafilomycin A1 can potentially
suppress CoNP-induced cytotoxicity and aseptic inflammation by inhibiting
intracellular corrosion of CoNPs and that the reduction in Co ions released from
CoNPs may play an important role in downregulating oxidative stress in RAW264.7
cells.
AN - WOS:000367101800014
AU - Wang, S. H.
AU - Liu, F.
AU - Zeng, Z. X.
AU - Yang, H. L.
AU - Jiang, H. T.
DA - JAN
DO - 10.1007/s12011-015-0381-9
IS - 1
PY - 2016
SN - 0163-4984
1559-0720
SP - 94-105
ST - The Protective Effect of Bafilomycin A1 Against Cobalt Nanoparticle-Induced
Cytotoxicity and Aseptic Inflammation in Macrophages In Vitro
TI - The Protective Effect of Bafilomycin A1 Against Cobalt Nanoparticle-Induced
Cytotoxicity and Aseptic Inflammation in Macrophages In Vitro
VL - 169
ID - 6271
ER -
TY - JOUR
AB - Diabetic foot ulcer (DFU) is one of the most serious complication of diabetes
mellitus with the characteristic of long-term non-healing wound, which often leads
to amputation and eventual death. In response to the refractory wound, medical
dressings are considered helpful. However, traditional dressings often have the
disadvantages of limited efficacy and difficulty in long-term maintenance,
therefore there is an urgent need to develop novel dressing supplements. A variety
of biomaterials have been used as wound dressings to promote wound repair. In
particular, the injectable hydrogels with 3D structures similar to soft tissue have
excellent biocompatibility, which can fill the irregular wound to play the effect
of moisturizing, anti-infection, etc. On this basis, the injectable hydrogels can
also be loaded with carriers with certain physiological and pharmacological
effects, such as inorganic nanoparticles, drugs, and bioactive carriers, to achieve
functions of antibacterial, anti-inflammatory, antioxidant, promoting collagen
deposition, and vascular regeneration. Therefore they can promote wound healing
well and have a great prospect in wound dressing. This review describes the
mechanisms of DFU, summarizes the research progress of injectable carrier hydrogel
systems in DFU repair, evaluates the advantages and disadvantages of various
materials and the therapeutic principles of carriers, and proposes the challenges
and development prospects of injectable carrier hydrogel.
AN - WOS:001023013200002
AU - Wang, S. W.
AU - Zhang, J.
AU - Zhou, W. L.
AU - Liu, W. T.
AU - Ou, Y.
AU - Zheng, X. X.
AU - Yang, H. Z.
AU - Wang, T. L.
C6 - JUL 2023
DA - JUL
DO - 10.1007/s10853-023-08730-x
IS - 28
PY - 2023
SN - 0022-2461
1573-4803
SP - 11441-11468
ST - Injectable carrier hydrogel for diabetic foot ulcer wound repair
T2 - JOURNAL OF MATERIALS SCIENCE
TI - Injectable carrier hydrogel for diabetic foot ulcer wound repair
VL - 58
ID - 6665
ER -
TY - JOUR
AB - Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture,
flavour or anti-microbial properties of various food products and packaging
materials. Despite their increased oral exposure, their potential toxicities in the
dysfunctional intestine are unclear. Here, the effects of ingested nAg or nTiO2 on
inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative
colitis. Mice (eight/group) were exposed to nAg or nTiO2 by oral gavage for 10
consecutive days. We characterized disease phenotypes, histology, and alterations
in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing). Oral
exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice
but induced extensive responses in gene expression enriching processes of apoptotic
cell death and RNA metabolism. Instead, ingested nTiO2 yielded shorter colon,
aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
Both nanoparticles significantly changed the gut microbiota composition, resulting
in loss of diversity and increase of potential pathobionts. They also increased
colonic mucus and abundance of Akkermansia muciniphila. Overall, nAg and nTiO2
induce dissimilar immunotoxicological changes at the molecular and microbiome level
in the context of colon inflammation. The results provide valuable information for
evaluation of utilizing metallic nanoparticles in food products for the vulnerable
population. © 2022 The Authors
AU - Wang, S.
AU - Kang, X.
AU - Alenius, H.
AU - Wong, S. H.
AU - Karisola, P.
AU - El-Nezami, H.
C7 - 113368
DB - Scopus
DO - 10.1016/j.fct.2022.113368
KW - 16S sequencing
DSS-Induced colitis
Gut microbiota
Inflammation
Metal oxides
Mouse model
Nanoparticles
RSEQ
Animals
Colitis
Colitis, Ulcerative
Colon
Dextran Sulfate
Mice
Mice, Inbred C57BL
Microbiota
RNA
Silver
Titanium
Transcriptome
silver nanoparticle
transcriptome
dextran sulfate
nanoparticle
silver
titanium
titanium dioxide
Akkermansia muciniphila
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
bioinformatics
cell infiltration
controlled study
differential gene expression
DNA extraction
feces analysis
gene expression
histology
inflammatory cell
intestine flora
microbial community
microbial diversity
mouse
nonhuman
RNA extraction
RNA metabolism
RNA sequence
RNA sequencing
transcriptomics
ulcerative colitis
animal
C57BL mouse
colitis
colon
disease model
metabolism
microflora
M3 - Article
PY - 2022
ST - Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and
microbiota in a mouse model of ulcerative colitis: Ag or TiO2 nanoparticles in
ulcerative colitis
TI - Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and
microbiota in a mouse model of ulcerative colitis: Ag or TiO2 nanoparticles in
ulcerative colitis
85137717219&doi=10.1016%2fj.fct.2022.113368&partnerID=40&md5=58289e753d66b37e9e603a
9e991520ac
VL - 169
ID - 4986
ER -
TY - JOUR
AB - Clinical application of antibiotic-free agents like silver nanoparticle-
derived materials remains a critical challenge due to their limited long-term
antibacterial activity and potential system toxicity. Herein, a highly
biocompatible Ag nanocluster-reinforced hydrogel with enhanced synergistic
antibacterial ability has been developed. Specifically, bioactive curcumin was
incorporated into lysozyme-protected ultra -small Ag nanoclusters (LC-AgNCs) and
further integrated with sodium alginate (Sa) hydrogel (LC-AgNCs@Sa) through
multiple interaction forces. Due to the synergistic antibacterial activity, LC-
AgNCs could effectively kill both S. aureus and E. coli bacteria with a
concentration down to 2.5 lg mL-1. In-depth mechanism investigations revealed that
the bactericidal effect of LC-AgNCs lies in their bacterial membrane destruction,
reactive oxygen species (ROS) production, glutathione depletion and prooxidant-
antioxidant system disruption ability. Curcumin can mediate the intracellular ROS
balance to protect NIH 3T3 cells from oxidative stress and improve the
biocompatibility of LC-AgNCs@Sa. LC-AgNCs@Sa with long-term antibacterial ability
can effectively protect the wound from bacterial inva-sion in vivo, and
significantly accelerate the wound healing process due to their distinctive
functions of inhibiting inflammatory factor (TNF-a) production, promoting collagen
deposit and facilitating re-epithelization. This study provides a new, versatile
strategy for the design of high-performance antibacte-rial dressing for broad
infectious disease therapy.(c) 2022 Elsevier Inc. All rights reserved.
AN - WOS:000901808400004
AU - Wang, T. Y.
AU - Li, Y. X.
AU - Liu, Y. N.
AU - Xu, Z. Q.
AU - Wen, M. Y.
AU - Zhang, L. B.
AU - Xue, Y. M.
AU - Shang, L.
C6 - DEC 2022
DA - MAR
DO - 10.1016/j.jcis.2022.11.139
PY - 2023
SN - 0021-9797
1095-7103
SP - 851-865
ST - Highly biocompatible Ag nanocluster-reinforced wound dressing with long-term
and synergistic bactericidal activity
TI - Highly biocompatible Ag nanocluster-reinforced wound dressing with long-term
VL - 633
ID - 6582
ER -
TY - JOUR
AB - Clinical application of antibiotic-free agents like silver nanoparticle-
derived materials remains a critical challenge due to their limited long-term
antibacterial activity and potential system toxicity. Herein, a highly
biocompatible Ag nanocluster-reinforced hydrogel with enhanced synergistic
antibacterial ability has been developed. Specifically, bioactive curcumin was
incorporated into lysozyme-protected ultrasmall Ag nanoclusters (LC-AgNCs) and
further integrated with sodium alginate (Sa) hydrogel (LC-AgNCs@Sa) through
multiple interaction forces. Due to the synergistic antibacterial activity, LC-
AgNCs could effectively kill both S. aureus and E. coli bacteria with a
concentration down to 2.5 μg mL−1. In-depth mechanism investigations revealed that
the bactericidal effect of LC-AgNCs lies in their bacterial membrane destruction,
reactive oxygen species (ROS) production, glutathione depletion and prooxidant-
antioxidant system disruption ability. Curcumin can mediate the intracellular ROS
balance to protect NIH 3T3 cells from oxidative stress and improve the
biocompatibility of LC-AgNCs@Sa. LC-AgNCs@Sa with long-term antibacterial ability
can effectively protect the wound from bacterial invasion in vivo, and
significantly accelerate the wound healing process due to their distinctive
functions of inhibiting inflammatory factor (TNF-α) production, promoting collagen
deposit and facilitating re-epithelization. This study provides a new, versatile
strategy for the design of high-performance antibacterial dressing for broad
infectious disease therapy. © 2022 Elsevier Inc.
AU - Wang, T.
AU - Li, Y.
AU - Liu, Y.
AU - Xu, Z.
AU - Wen, M.
AU - Zhang, L.
AU - Xue, Y.
AU - Shang, L.
DB - Scopus
DO - 10.1016/j.jcis.2022.11.139
KW - Ag nanoclusters
Hydrogel
Infected-wound healing
Oxidative stress
Synergistic antibacterial
Animals
Bandages
Curcumin
Escherichia coli
Hydrogels
Metal Nanoparticles
Mice
Silver
Biocompatibility
Disease control
Nanoclusters
Reinforcement
Silver compounds
Sodium alginate
alginic acid
collagen
curcumin
glutathione
hydrogel
lysozyme
malonaldehyde
silver nanoparticle
antiinfective agent
metal nanoparticle
silver
Antibacterials
Infected wounds
Ultra-small
Wound healing
animal cell
animal experiment
animal model
animal tissue
Article
bacterial infection
bacterial membrane
biocompatibility
cell protection
collagen synthesis
controlled study
depletion
epithelization
granulation tissue
in vitro study
inflammation
membrane damage
mouse
nanofabrication
NIH 3T3 cell line
nonhuman
oxidative stress
wound
wound healing
wound infection
animal
bandage
pharmacology
M3 - Article
PY - 2023
SP - 851-865
ST - Highly biocompatible Ag nanocluster-reinforced wound dressing with long-term
T2 - Journal of Colloid and Interface Science
TI - Highly biocompatible Ag nanocluster-reinforced wound dressing with long-term
85144043049&doi=10.1016%2fj.jcis.2022.11.139&partnerID=40&md5=ac4f184ffa35fd2fce8c8
932c154609c
VL - 633
ID - 5022
ER -
TY - JOUR
AB - Pathogen infections impose severe challenges in clinical practice, especially
for patients infected with antibiotic-resistant microbes. The thioredoxin (Trx)
system in Gram-positive bacteria serves as an ideal antimicrobial target for novel
medicine design due to the structural differences from corresponding system in
mammals. However, a backup thiol-dependent antioxidant glutathione (GSH) system
limits the effectiveness of drugs in many Gram-negative bacteria. Herein, we
synthesize a thiol-targeting nanoinhibitor based on an enzyme-responsive covalent
organic framework (COF) coloaded with silver nanoparticles (AgNPs) and ebselen
(EBS) (Ag-TA-CON@EBS@PEG) to exert synergistic antibacterial effects. Since
azoreductase can dissociate the enzyme-responsive COF, we adopt this strategy to
achieve the accurate release of EBS and Ag+ at infection sites. Our research
identifies that the functionalized nanoinhibitor shows excellent bactericidal
performance for Gram-positive and Gram-negative bacteria in vitro and exhibits low
toxicity to normal cells. Besides, the nanoinhibitor presents favorable
biocompatibility, anti-inflammatory property, and effective wound healing ability
in mice. This paper provides a promising clinical strategy for synergistic
antibacterial therapy and enhanced wound healing properties via an optimized
combination of the targeted nanomedicines with an intelligent drug conveying
platform.
AN - WOS:000846712500001
AU - Wang, X. Y.
AU - Sun, B. H.
AU - Ye, Z. Q.
AU - Zhang, W. J.
AU - Xu, W.
AU - Gao, S. R.
AU - Zhou, N. L.
AU - Wu, F.
AU - Shen, J.
C6 - AUG 2022
DA - AUG 31
DO - 10.1021/acsami.2c08845
IS - 34
PY - 2022
SN - 1944-8244
1944-8252
SP - 38483-38496
ST - Enzyme-Responsive COF-Based Thiol-Targeting Nanoinhibitor for Curing
TI - Enzyme-Responsive COF-Based Thiol-Targeting Nanoinhibitor for Curing
VL - 14
ID - 6319
ER -
TY - JOUR
AB - Nanocellulose is increasingly considered for applications; however, the
fibrillar nature, crystalline phase, and surface reactivity of these high aspect
ratio nanomaterials need to be considered for safe biomedical use. Here a
comprehensive analysis of the impact of cellulose nanofibrils (CNF) and
nanocrystals (CNC) is performed using materials provided by the Nanomaterial Health
Implications Research Consortium of the National Institute of Environmental Health
Sciences. An intermediary length of nanocrystals is also derived by acid
hydrolysis. While all CNFs and CNCs are devoid of cytotoxicity, 210 and 280 nm
fluorescein isothiocyanate (FITC)-labeled CNCs show higher cellular uptake than
longer and shorter CNCs or CNFs. Moreover, CNCs in the 200-300 nm length scale are
more likely to induce lysosomal damage, NLRP3 inflammasome activation, and IL-1
beta production than CNFs. The pro-inflammatory effects of CNCs are correlated with
higher crystallinity index, surface hydroxyl density, and reactive oxygen species
generation. In addition, CNFs and CNCs can induce maturation of bone marrow-derived
dendritic cells and CNCs (and to a lesser extent CNFs) are found to exert adjuvant
effects in ovalbumin (OVA)-injected mice, particularly for 210 and 280 nm CNCs. All
considered, the data demonstrate the importance of length scale, crystallinity, and
surface reactivity in shaping the innate immune response to nanocellulose.
AN - WOS:000483950500001
AU - Wang, X.
AU - Chang, C. H.
AU - Jiang, J. H.
AU - Liu, Q.
AU - Liao, Y. P.
AU - Lu, J. Q.
AU - Li, L. J.
AU - Liu, X. S.
AU - Kim, J.
AU - Ahmed, A.
AU - Nel, A. E.
AU - Xia, T.
C6 - AUG 2019
C7 - 1901642
DA - OCT
DO - 10.1002/smll.201901642
IS - 42
PY - 2019
SN - 1613-6810
1613-6829
ST - The Crystallinity and Aspect Ratio of Cellulose Nanomaterials Determine Their
Pro-Inflammatory and Immune Adjuvant Effects In Vitro and In Vivo
T2 - SMALL
TI - The Crystallinity and Aspect Ratio of Cellulose Nanomaterials Determine Their
Pro-Inflammatory and Immune Adjuvant Effects In Vitro and In Vivo
VL - 15
ID - 6602
ER -
TY - JOUR
AB - Quantum dots (QDs) are nanoparticles with a particle size of 1–10 nm. Typical
QDs are made of compounds such as cadmium sulfide, cadmium selenide, silver
sulfide, and indium phosphide, among others. QDs exhibit promising potential for a
wide range of applications owing to their excellent optical properties. With the
rise in the application of and demand for QDs, QDs accumulation in the environment
has increased markedly. QDs enter the pulmonary system via inhalation and trigger
pulmonary toxicity. This paper first reviews the pulmonary toxicity of different
types of QDs in vivo and in vitro. Regarding acute toxicity, QDs cause changes in
cell morphology, cell membrane disruption, cell viability, and pulmonary
inflammation. Regarding chronic toxicity, cadmium-based QDs cause pulmonary
granulomas and have a potential carcinogenic risk. Second, this paper presents an
overview of the pulmonary toxicity mechanism of QDs, involving oxidative stress,
inflammation, autophagy, apoptosis, and ferroptosis. It summarizes mitogen-
activated protein kinases, nuclear factor κB, nuclear factor-erythroid 2-related
factor 2, P53, and Phosphoinositide 3-kinase/AKT signaling pathways in apoptosis
and autophagy. Third, it enumerates the physicochemical properties of QDs
influencing pulmonary toxicity, ranging from components, surface functional groups,
size, and surface charge. Lastly, it outlines the shortcomings of current studies
on QDs pulmonary toxicity. The paper concludes with a recommendation discussing
research-based improvements in the physicochemical properties of QDs to reduce
their release in the environment. © 2022 Elsevier B.V.
AU - Wang, X.
AU - He, K.
AU - Hu, Y.
AU - Tang, M.
C7 - 110247
DB - Scopus
DO - 10.1016/j.cbi.2022.110247
KW - Mechanism
Pulmonary
Quantum dots
Toxicity
Cell Survival
Nanoparticles
Particle Size
Phosphatidylinositol 3-Kinases
Quantum Dots
cadmium
CXCL1 chemokine
CXCL2 chemokine
doxorubicin
epithelial derived neutrophil activating factor 78
indium
interleukin 1beta
interleukin 33
interleukin 6
macrogol
nanoparticle
protein p53
quantum dot
selenium
silica nanoparticle
silver
silver sulfide
synaptophysin
unclassified drug
zinc oxide
acute toxicity
apoptosis
biology
cell membrane
cell structure
cell viability
chronic toxicity
environment
ferroptosis
human
inflammation
lung
lung toxicity
lymphocyte
macrophage
neutrophil
Nrf2 signaling
oxidative stress
particle size
photoluminescence
physical chemistry
physical phenomena
pneumonia
protein aggregation
review
Review
surface charge
cell survival
chemistry
M3 - Review
PY - 2022
ST - A review of pulmonary toxicity of different types of quantum dots in
environmental and biological systems
TI - A review of pulmonary toxicity of different types of quantum dots in
environmental and biological systems
85140952305&doi=10.1016%2fj.cbi.2022.110247&partnerID=40&md5=8dbc0443a4cf41470970a2
aae642e098
VL - 368
ID - 5047
ER -
TY - JOUR
AB - Pathogen infections impose severe challenges in clinical practice, especially
for patients infected with antibiotic-resistant microbes. The thioredoxin (Trx)
system in Gram-positive bacteria serves as an ideal antimicrobial target for novel
medicine design due to the structural differences from corresponding system in
mammals. However, a backup thiol-dependent antioxidant glutathione (GSH) system
limits the effectiveness of drugs in many Gram-negative bacteria. Herein, we
synthesize a thiol-targeting nanoinhibitor based on an enzyme-responsive covalent
organic framework (COF) coloaded with silver nanoparticles (AgNPs) and ebselen
(EBS) (Ag-TA-CON@EBS@PEG) to exert synergistic antibacterial effects. Since
azoreductase can dissociate the enzyme-responsive COF, we adopt this strategy to
achieve the accurate release of EBS and Ag+ at infection sites. Our research
identifies that the functionalized nanoinhibitor shows excellent bactericidal
performance for Gram-positive and Gram-negative bacteria in vitro and exhibits low
toxicity to normal cells. Besides, the nanoinhibitor presents favorable
biocompatibility, anti-inflammatory property, and effective wound healing ability
in mice. This paper provides a promising clinical strategy for synergistic
antibacterial therapy and enhanced wound healing properties via an optimized
combination of the targeted nanomedicines with an intelligent drug conveying
platform. ©
AU - Wang, X.
AU - Sun, B.
AU - Ye, Z.
AU - Zhang, W.
AU - Xu, W.
AU - Gao, S.
AU - Zhou, N.
AU - Wu, F.
AU - Shen, J.
DB - Scopus
DO - 10.1021/acsami.2c08845
covalent organic framework
enzyme response
nanoinhibitor
thiol-dependent system
Bacteria
Biocompatibility
Conveying
Enzyme activity
Silver compounds
Covalent organic frameworks
Ebselen
Enzyme response
Gram-negative bacteria
Nanoinhibitor
Pathogen infection
Thiol-dependent system
Wound healing
Mammals
M3 - Article
PY - 2022
ST - Enzyme-Responsive COF-Based Thiol-Targeting Nanoinhibitor for Curing
TI - Enzyme-Responsive COF-Based Thiol-Targeting Nanoinhibitor for Curing
85137137225&doi=10.1021%2facsami.2c08845&partnerID=40&md5=aa66973c290b624bc8350be09
4f226f1
ID - 5142
ER -
TY - JOUR
AB - Background: Burn Liniment (BL) is a popular traditional Chinese medicine
formula consisting five herbal medicines (Flos Lonicerae, Rhizoma Polygoni
Cuspidati, Pericarpium Granati, Terminalia chebula Retz. and Galla Chinensis), that
has been used in China for centuries to cure burn. This study investigated the
healing effect of BL on deep second degree burn wounds in rats. Materials and
methods: The animals were divided into four groups including control group, model
group, 1% silver sulfadiazine (SSD) group and BL group. On days 0,3,7,14 and 21,
animal weight, wound area as well as histo-pathological observations of the skin
were evaluated in different groups. Serum anti-intercellular adhesion molecule
1(ICAM-1), IL-10 levels and myeloperoxidase (MPO) activity were measured on the
21st day. HPLC chromatography of BL was prepared and concentrations of active
constituents were determined. Antibacterial test and toxicological test were also
performed. Results: The average wound area of BL treatment group was also
significantly smaller than model control rats on days 14 and 21. Serum anti-
intercellular adhesion molecule 1(ICAM-1) levels and myeloperoxidase (MPO) activity
of BL group decreased significantly than in model rats on day 21 while IL-10 level
of BL group increased remarkably than in model rats on the 21st day, showing that
BL has strong anti-inflammatory activity on burned rats. The histological studies
indicated that inflammatory cells disappeared significantly and were replaced by
new granulation tissue, and epithelialization progressed quickly and was treated
with BL on the 21st day. Meanwhile, HPLC chromatography of BL was prepared and
concentration of Chlorogenic acid, Polydatin and Gallic acid from BL were
determined. Antibacterial test revealed that the MIC of BL on Staphyloccocus
aureus, Pseudomonas aeruginosa and Escherichia coli were 1.56, 6.25 and 1.56
mg.mL(-1) respectively. Toxicological test showed that BL does not induce skin
irritation or sensitivity signs and has no acute toxicity reaction. Conclusions:
Our study revealed that BL could enhance cutaneous burn wound healing effectively.
It also showed strong anti-inflammatory and antibacterial activity in rats.
AN - WOS:000345923100010
AU - Wang, X.
AU - Zhao, Q. S.
AU - Zhao, C. L.
AU - Guo, H.
AU - Peng, S. W.
AU - Wu, J. H.
DO - 10.4314/ajtcam.v11i6.10
IS - 6
PY - 2014
SN - 0189-6016
SP - 92-104
ST - EFFECT OF CHINESE MEDICAL HERBS- BURN LINIMENT ON DEEP SECOND DEGREE BURN IN
RATS
T2 - AFRICAN JOURNAL OF TRADITIONAL COMPLEMENTARY AND ALTERNATIVE MEDICINES
TI - EFFECT OF CHINESE MEDICAL HERBS- BURN LINIMENT ON DEEP SECOND DEGREE BURN IN
RATS
VL - 11
ID - 6337
ER -
TY - JOUR
AB - Background: Burn Liniment (BL) is a popular traditional Chinese medicine
formula consisting five herbal medicines (Flos Lonicerae, Rhizoma Polygoni
Cuspidati, Pericarpium Granati,Terminalia chebula Retz. and Galla Chinensis), that
has been used in China for centuries to cure burn. This study investigated the
healing effect of BL on deep second degree burn wounds in rats. Materials and
methods: The animals were divided into four groups including control group, model
group,1% silver sulfadiazine (SSD) group and BL group. On days 0,3,7,14 and
21,animal weight, wound area as well as histo-pathological observations of the skin
were evaluated in different groups. Serum anti-intercellular adhesion molecule
1(ICAM-1), IL-10 levels and myeloperoxidase (MPO) activity were measured on the
21st day. HPLC chromatography of BL was prepared and concentrations of active
constituents were determined. Antibacterial test and toxicological test were also
performed. Results: The average wound area of BL treatment group was also
significantly smaller than model control rats on days 14 and 21. Serum anti-
intercellular adhesion molecule 1(ICAM-1) levels and myeloperoxidase (MPO) activity
of BL group decreased significantly than in model rats on day 21 while IL-10 level
of BL group increased remarkably than in model rats on the 21st day, showing that
BL has strong anti-inflammatory activity on burned rats. The histological studies
indicated that inflammatory cells disappeared significantly and were replaced by
new granulation tissue, and epithelialization progressed quickly and was treated
with BL on the 21st day. Meanwhile, HPLC chromatography of BL was prepared and
concentration of Chlorogenic acid, Polydatin and Gallic acid from BL were
determined. Antibacterial test revealed that the MIC of BL on Staphyloccocus
aureus, Pseudomonas aeruginosa and Escherichia coli were 1.56, 6.25 and 1.56 mg·mL-
1 respectively. Toxicological test showed that BL does not induce skin irritation
or sensitivity signs and has no acute toxicity reaction. Conclusions: Our study
revealed that BL could enhance cutaneous burn wound healing effectively. It also
showed strong anti-inflammatory and antibacterial activity in rats. © 2014, African
Ethnomedicines Network. All right reserved.
AU - Wang, X.
AU - Zhao, Q. S.
AU - Zhao, C. L.
AU - Guo, H.
AU - Peng, S. W.
AU - Wu, J. H.
DB - Scopus
DO - 10.4314/ajtcam.v11i6.10
IS - 6
Antibacterial
Burn Liniment
Burn wound
Deep second degree
Toxicological test
chlorogenic acid
gallic acid
herbaceous agent
interleukin 10
myeloperoxidase
polydatin
sulfadiazine silver
unclassified drug
animal experiment
animal model
animal tissue
Article
blood level
body weight
burn
controlled study
drug megadose
epithelization
Escherichia coli
Galla chinensis
granulation tissue
herb
Lonicera
low drug dose
nonhuman
Pericarpium granati
Polygonum cuspidatum
rat
Terminalia chebula
toxicity testing
wound healing
M3 - Article
PY - 2014
SP - 92-104
ST - Effect of chinese medical herbs-burn liniment on deep second degree burn in
rats
T2 - African Journal of Traditional, Complementary and Alternative Medicines
TI - Effect of chinese medical herbs-burn liniment on deep second degree burn in
rats
84923911088&doi=10.4314%2fajtcam.v11i6.10&partnerID=40&md5=d90728fe196befbabe981770
ac3768de
VL - 11
ID - 5580
ER -
TY - JOUR
AB - Keratin based biomaterials have emerged as potential candidates for various
biomedical and biotechnological applications due to their intrinsic
biocompatibility, biodegradability, mechanical durability, and natural abundance.
The objective of this study is to combine the merits of polyurethane, keratin, and
silver nanoparticles (AgNPs) together and develop a novel nanofibrous mat for wound
dressing. Herein, keratin was first extracted from human hair and chemically
modified with iodoacetic acid to afford S-(carboxymethyl) keratin. The modified
keratin was examined using Raman spectroscopy, infrared spectroscopy, and SDS-PAGE.
The keratin was then blended with polyurethane (PU) and electrospun. Subsequently,
AgNPs were formed in situ to afford antibacterial PU/keratin/AgNP mats. These mats
were characterized using field emission scanning electron microscopy (FE-SEM),
attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR),
water contact angle measurements, and X-ray photoelectron spectroscopy (XPS). MTT
results indicated that the introduction of keratin could accelerate fibroblast cell
proliferation, while the loaded AgNPs did not weaken cytocompatibility.
Antibacterial test results showed that PU/keratin/AgNP mats exerted good
antibacterial property. The results from a wound healing test and a histological
examination suggested that these biocomposite mats could remarkably accelerate
wound recovery as compared to the conventional gauze sponge dressing. Given their
excellent biocompatibility, antibacterial properties, and very mild inflammatory
responses, PU/keratin/AgNP mats have great potential for wound dressing
applications.
AN - WOS:000369606700007
AU - Wang, Y. F.
AU - Li, P. F.
AU - Xiang, P.
AU - Lu, J. T.
AU - Yuan, J.
AU - Shen, J.
DO - 10.1039/c5tb02358k
IS - 4
PY - 2016
SN - 2050-750X
2050-7518
SP - 635-648
ST - Electrospun polyurethane/keratin/AgNP biocomposite mats for biocompatible and
antibacterial wound dressings
TI - Electrospun polyurethane/keratin/AgNP biocomposite mats for biocompatible and
VL - 4
ID - 6448
ER -
TY - JOUR
AB - Periodontitis is a chronic inflammatory disease initiated by pathogenic
biofilms and host immunity that damages tooth-supporting tissues, including the
gingiva, periodontal ligament and alveolar bone. The physiological functions of the
oral cavity, such as saliva secretion and chewing, greatly reduce the residence of
therapeutic drugs in the area of a periodontal lesion. In addition, complex and
diverse pathogenic mechanisms make effectively treating periodontitis difficult.
Therefore, designing advanced local drug delivery systems and rational therapeutic
strategies are the basis for successful periodontitis treatment. Hydrogels have
attracted considerable interest in the field of periodontitis treatment due to
their biocompatibility, biodegradability and convenient administration to the
periodontal pocket. In recent years, the focus of hydrogel research has shifted to
smart stimuli-responsive hydrogels, which can undergo flexible sol-gel transitions
in situ and control drug release in response to stimulation by temperature, light,
pH, ROS, glucose, or enzymes. In this review, we systematically introduce the
development and rational design of emerging smart stimuli-responsive hydrogels for
periodontitis treatment. We also discuss the state-of-the-art therapeutic
strategies of smart hydrogels based on the patho-genesis of periodontitis.
Additionally, the challenges and future research directions of smart hydrogels for
periodontitis treatment are discussed from the perspective of developing efficient
hydrogel delivery systems and potential clinical applications.
AN - WOS:000987808200001
AU - Wang, Y. X.
AU - Li, J. X.
AU - Tang, M. M.
AU - Peng, C. J.
AU - Wang, G. C.
AU - Wang, J. J.
AU - Wang, X. R.
AU - Chang, X. W.
AU - Guo, J.
AU - Gui, S. Y.
C6 - APR 2023
C7 - 114688
DA - JUN
DO - 10.1016/j.biopha.2023.114688
PY - 2023
SN - 0753-3322
1950-6007
ST - Smart stimuli-responsive hydrogels for drug delivery in periodontitis
treatment
TI - Smart stimuli-responsive hydrogels for drug delivery in periodontitis
treatment
VL - 162
ID - 6764
ER -
TY - JOUR
AB - Keratin based biomaterials have emerged as potential candidates for various
biomedical and biotechnological applications due to their intrinsic
biocompatibility, biodegradability, mechanical durability, and natural abundance.
The objective of this study is to combine the merits of polyurethane, keratin, and
silver nanoparticles (AgNPs) together and develop a novel nanofibrous mat for wound
dressing. Herein, keratin was first extracted from human hair and chemically
modified with iodoacetic acid to afford S-(carboxymethyl) keratin. The modified
keratin was examined using Raman spectroscopy, infrared spectroscopy, and SDS-PAGE.
The keratin was then blended with polyurethane (PU) and electrospun. Subsequently,
AgNPs were formed in situ to afford antibacterial PU/keratin/AgNP mats. These mats
were characterized using field emission scanning electron microscopy (FE-SEM),
attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR),
water contact angle measurements, and X-ray photoelectron spectroscopy (XPS). MTT
results indicated that the introduction of keratin could accelerate fibroblast cell
proliferation, while the loaded AgNPs did not weaken cytocompatibility.
Antibacterial test results showed that PU/keratin/AgNP mats exerted good
antibacterial property. The results from a wound healing test and a histological
examination suggested that these biocomposite mats could remarkably accelerate
wound recovery as compared to the conventional gauze sponge dressing. Given their
excellent biocompatibility, antibacterial properties, and very mild inflammatory
responses, PU/keratin/AgNP mats have great potential for wound dressing
applications. © 2015 The Royal Society of Chemistry.
AU - Wang, Y.
AU - Li, P.
AU - Xiang, P.
AU - Lu, J.
AU - Yuan, J.
AU - Shen, J.
DB - Scopus
DO - 10.1039/c5tb02358k
IS - 4
Biodegradation
Biomaterials
Cell culture
Cell proliferation
Composite materials
Enamels
Keratin
Polyurethanes
Silver
Attenuated total reflection Fourier transform infrared spectroscopy
Biotechnological applications
Histological examination
Mechanical durability
Water contact angle measurement
M3 - Article
PY - 2016
SP - 635-648
ST - Electrospun polyurethane/keratin/AgNP biocomposite mats for biocompatible and
TI - Electrospun polyurethane/keratin/AgNP biocomposite mats for biocompatible and
84955490917&doi=10.1039%2fc5tb02358k&partnerID=40&md5=db02fa3522408371e6bc7c0433a1a
18e
VL - 4
ID - 5577
ER -
TY - JOUR
AB - Stimuli-responsive hydrogels possess unique advantages in drug delivery due
to their variable performance and status based on the external environment. In the
present study, a dual-responsive (pH and reactive oxygen species (ROS)) hydrogel
was prepared to realize drug release properties under inflammatory stimulation. By
grafting 3-carboxy-phenylboronic acid to the gelatin molecular backbone and cross-
linking with poly(vinyl alcohol), we successfully synthesized the inflammation-
responsive drug-loaded hydrogels after encapsulation with vancomycin-conjugated
silver nanoclusters (VAN-AgNCs) and pH-sensitive micelles loaded with nimesulide
(NIM). This novel design not only retained the dynamic functions of hydrogels, such
as injectability, self-healing, and remodeling, but also realized sequential and
on-demand drug delivery at diabetic-infected wound sites. In this work, we found
that the hydrogel exhibited excellent biocompatibility and hemostasis properties
owing to the enhanced cell-adhesive property of the gelatin component. The
significant antibacterial and anti-inflammatory effect of the hydrogel was
demonstrated in an in vitro experiment. Moreover, in the in vivo experiment, the
hydrogel was found to play a role in promoting infected wound healing through
sequential hemostasis and anti-bacterial and anti-inflammatory processes.
Collectively, this inflammation-responsive hydrogel design containing VAN-AgNCs and
NIM-loaded micelles has great potential in the application of chronically infected
diabetic wound treatment, as well as in other inflammatory diseases.
AN - WOS:000677540900095
AU - Wang, Y.
AU - Wu, Y.
AU - Long, L. Y.
AU - Yang, L.
AU - Fu, D. H.
AU - Hu, C.
AU - Kong, Q. Q.
AU - Wang, Y. B.
C6 - JUL 2021
DA - JUL 21
DO - 10.1021/acsami.1c09889
IS - 28
PY - 2021
SN - 1944-8244
1944-8252
SP - 33584-33599
ST - Inflammation-Responsive Drug-Loaded Hydrogels with Sequential Hemostasis,
Antibacterial, and Anti-Inflammatory Behavior for Chronically Infected Diabetic
Wound Treatment
TI - Inflammation-Responsive Drug-Loaded Hydrogels with Sequential Hemostasis,
Wound Treatment
VL - 13
ID - 6218
ER -
TY - JOUR
AB - Stimuli-responsive hydrogels possess unique advantages in drug delivery due
to their variable performance and status based on the external environment. In the
present study, a dual-responsive (pH and reactive oxygen species (ROS)) hydrogel
was prepared to realize drug release properties under inflammatory stimulation. By
grafting 3-carboxy-phenylboronic acid to the gelatin molecular backbone and cross-
linking with poly(vinyl alcohol), we successfully synthesized the inflammation-
responsive drug-loaded hydrogels after encapsulation with vancomycin-conjugated
silver nanoclusters (VAN-AgNCs) and pH-sensitive micelles loaded with nimesulide
(NIM). This novel design not only retained the dynamic functions of hydrogels, such
as injectability, self-healing, and remodeling, but also realized sequential and
on-demand drug delivery at diabetic-infected wound sites. In this work, we found
that the hydrogel exhibited excellent biocompatibility and hemostasis properties
owing to the enhanced cell-adhesive property of the gelatin component. The
significant antibacterial and anti-inflammatory effect of the hydrogel was
demonstrated in an in vitro experiment. Moreover, in the in vivo experiment, the
hydrogel was found to play a role in promoting infected wound healing through
sequential hemostasis and antibacterial and anti-inflammatory processes.
Collectively, this inflammation-responsive hydrogel design containing VAN-AgNCs and
NIM-loaded micelles has great potential in the application of chronically infected
diabetic wound treatment, as well as in other inflammatory diseases. © 2021
AU - Wang, Y.
AU - Wu, Y.
AU - Long, L.
AU - Yang, L.
AU - Fu, D.
AU - Hu, C.
AU - Kong, Q.
DB - Scopus
DO - 10.1021/acsami.1c09889
IS - 28
KW - antibacterial hybrid
drug-loaded micelles
hemostasis
inflammation-responsive
wound healing
Animals
Boronic Acids
Drug Carriers
Gelatin
Hemostasis
Hemostatics
Hydrogels
Hydrogen-Ion Concentration
Male
Metal Nanoparticles
Polyvinyl Alcohol
Silver
Sulfonamides
Swine
Vancomycin
Wound Healing
Adhesives
Biocompatibility
Diseases
Micelles
Pathology
Polyvinyl alcohols
Tissue regeneration
antiinfective agent
boronic acid derivative
drug carrier
gelatin
hemostatic agent
metal nanoparticle
nimesulide
polyvinyl alcohol
silver
sulfonamide
vancomycin
Cell-adhesive properties
Drug release properties
External environments
Inflammatory disease
Inflammatory stimulation
On-demand drug delivery
animal
chemistry
complication
drug effect
hydrogel
male
pH
pig
Sprague Dawley rat
M3 - Article
PY - 2021
SP - 33584-33599
ST - Inflammation-Responsive Drug-Loaded Hydrogels with Sequential Hemostasis,
Wound Treatment
TI - Inflammation-Responsive Drug-Loaded Hydrogels with Sequential Hemostasis,
Wound Treatment
85111220580&doi=10.1021%2facsami.1c09889&partnerID=40&md5=dde6b78e0555dedeca8566862
6f84b68
VL - 13
ID - 5214
ER -
TY - JOUR
AB - Methicillin-resistant Staphylococcus (MRS) is a multi-drug resistant bacteria
that pose a serious threat to human health. Antibacterial nanomaterials are
becoming a promising antibiotic substitute or antibiotic adjuvants. In this work,
selenium nanowires were modified with nano???silver (Ag NPs) with antibacterial
activity and [Ru (bpy)2dppz]2+ with fluorescent labeling of DNA (SRA), and the
antibacterial activity, antibacterial mechanism and biological toxicity of SRA
synergistic antibiotics were studied. In vitro, antibacterial results show that SRA
(12 ??g/mL) improves the antibacterial activity of various antibiotics against
resistant bacteria and significantly slows the development of bacterial resistance
to antibiotics. Studies on antibacterial mechanisms have shown that SRA synergistic
antibiotics destroy drug-resistant bacteria through a combination of physical
(physical damage) and chemical pathways (destruction of biofilm, membrane
depolarization, cell membrane destruction, adenosine triphosphate consumption and
reactive oxygen species production). Transcriptomics analysis found that SRA
affects bacterial activity by affecting bacterial biosynthesis, ATP synthesis and
biofilm formation. Furthermore, SRA synergistic antibiotics can accelerate wound
healing of bacterial infection by reducing the inflammatory response. The toxicity
evaluation results show that SRA has extremely low cellular and in vivo toxicity.
SRA has the potential of clinical application as multiple antibiotic adjuvants to
deal with resistant bacterial infections.
AN - WOS:000811748600003
AU - Wang, Z. K.
AU - Yin, C. Y.
AU - Gao, Y.
AU - Liao, Z. Y.
AU - Li, Y. Q.
AU - Wang, W. Y.
AU - Sun, D. D.
C6 - MAY 2022
C7 - 212815
DA - JUN
DO - 10.1016/j.bioadv.2022.212815
PY - 2022
SN - 2772-9508
ST - Novel functionalized selenium nanowires as antibiotic adjuvants in multiple
ways to overcome drug resistance of multidrug-resistant bacteria
T2 - BIOMATERIALS ADVANCES
TI - Novel functionalized selenium nanowires as antibiotic adjuvants in multiple
VL - 137
ID - 6641
ER -
TY - JOUR
AB - Methicillin-resistant Staphylococcus (MRS) is a multi-drug resistant bacteria
that pose a serious threat to human health. Antibacterial nanomaterials are
becoming a promising antibiotic substitute or antibiotic adjuvants. In this work,
selenium nanowires were modified with nano-silver (Ag NPs) with antibacterial
activity and [Ru(bpy)2dppz]2+ with fluorescent labeling of DNA (SRA), and the
antibacterial activity, antibacterial mechanism and biological toxicity of SRA
synergistic antibiotics were studied. In vitro, antibacterial results show that SRA
(12 μg/mL) improves the antibacterial activity of various antibiotics against
resistant bacteria and significantly slows the development of bacterial resistance
to antibiotics. Studies on antibacterial mechanisms have shown that SRA synergistic
antibiotics destroy drug-resistant bacteria through a combination of physical
(physical damage) and chemical pathways (destruction of biofilm, membrane
depolarization, cell membrane destruction, adenosine triphosphate consumption and
reactive oxygen species production). Transcriptomics analysis found that SRA
affects bacterial activity by affecting bacterial biosynthesis, ATP synthesis and
biofilm formation. Furthermore, SRA synergistic antibiotics can accelerate wound
healing of bacterial infection by reducing the inflammatory response. The toxicity
evaluation results show that SRA has extremely low cellular and in vivo toxicity.
SRA has the potential of clinical application as multiple antibiotic adjuvants to
deal with resistant bacterial infections. © 2022 Elsevier B.V.
AU - Wang, Z.
AU - Yin, C.
AU - Gao, Y.
AU - Liao, Z.
AU - Li, Y.
AU - Wang, W.
AU - Sun, D.
C7 - 212815
DB - Scopus
DO - 10.1016/j.bioadv.2022.212815
KW - Antibacterial mechanism
Antibiotic adjuvant
Biofilm
Nanomaterials
Bacteria
Humans
Nanowires
Selenium
Adenosinetriphosphate
Antibiotics
Biochemistry
Cytology
Health risks
Toxicity
antiinfective agent
nanowire
selenium
Antibacterial mechanisms
Antibacterials
Antibiotics resistance
Drug-resistance
Drug-resistant bacteria
Functionalized
Resistant bacteria
bacterial infection
bacterium
human
Biofilms
M3 - Article
PY - 2022
ST - Novel functionalized selenium nanowires as antibiotic adjuvants in multiple
T2 - Biomaterials Advances
TI - Novel functionalized selenium nanowires as antibiotic adjuvants in multiple
85132556080&doi=10.1016%2fj.bioadv.2022.212815&partnerID=40&md5=dacadf9b1844e98cf79
a791a244e0120
VL - 137
ID - 5135
ER -
TY - JOUR
AB - Aims: To study the histological spectrum of lymphadenopathy in human
immunodeficiency virus (HIV) infected Thai patients. Methods: Lymph nodes from 55
HIV infected patients were accessioned over a 19 month period in two pathology
laboratories in Bangkok, Thailand. These were examined with H&E, Ziehl-Neelsen,
periodic acid-Schiff (PAS), PAS with diastase (PAS/D), Gram and methenamine stains.
Results: Six reaction patterns were observed: (1) classic necrotising granulomas
(30 cases); (2) extensive necrosis with minimal granulomatous response (5 cases);
(3) sarcoid-like non-necrotising granulomas (5 cases); (4) foamy macrophage or
pseudo-Gaucher cell response (5 cases); (5) inflammatory pseudotumour-like
proliferation (3 cases); and (6) non-specific lymphoid hyperplasia (7 cases).
Myriads of intracellular, long, slender acid-fast bacilli were found in those cases
with the pseudo-Gaucher cell and inflammatory pseudotumour-like response, while
variable numbers of bacilli were identified in those cases with non-necrotising
sarcoid-like granulomas. Few scattered acid-fast bacilli were found in five cases
with necrotising granulomas. In one case, yeast-like organisms in keeping with
Cryptococcus were identified. No organisms were identified in the cases showing
lymphoid hyperplasia, extensive necrosis and minimal granulomatous response, and in
the remaining cases of classic necrotising granulomas. Conclusions: The wide
spectrum of histological changes in HIV-associated lymphadenomegaly requires
recognition, particularly as the majority were associated with acid-fast organisms,
mostly in keeping with the morphological features of Mycobacterium avium-M.
intracellulare complex that was distinctively stained by Grocott methenamine-
silver, Gram and PAS stains. The histological changes mimic those of infarction and
other infective lymphadenitis, sarcoidosis, Whipple's disease, inflammatory
pseudotumour and spindle cell neoplasms. © 2007 Royal College of Pathologists of
Australasia.
AU - Wannakrairot, P.
AU - Leong, T. Y. M.
AU - Leong, A. S. Y.
DB - Scopus
DO - 10.1080/00313020701230674
IS - 2
KW - Apoptosis
Caseous necrosis
Granulomatous inflammation
Histoid reaction
HIV
Inflammatory pseudotumour
Lymph node
Mycobacteria
Pseudo-Gaucher cells
AIDS-Related Opportunistic Infections
HIV Infections
Humans
Lymphatic Diseases
Mycobacterium avium Complex
Mycobacterium avium-intracellulare Infection
Thailand
acid fast bacterium
article
bacterium detection
cell type
clinical feature
Cryptococcus
Gram staining
granuloma
histopathology
human
Human immunodeficiency virus infected patient
human tissue
intestine lipodystrophy
lymph node hyperplasia
mucocutaneous lymph node syndrome
Mycobacterium intracellulare avium
pseudotumor
sarcoidosis
spindle cell carcinoma
tissue section
M3 - Article
PY - 2007
SP - 223-227
ST - The morphological spectrum of lymphadenopathy in HIV infected patients
T2 - Pathology
TI - The morphological spectrum of lymphadenopathy in HIV infected patients
33947328869&doi=10.1080%2f00313020701230674&partnerID=40&md5=d506dd8339e7f5d9def694
56a0141203
VL - 39
ID - 5779
ER -
TY - JOUR
AB - The problem of multidrug resistance in bacterial pathogens is significant and
is related to the high morbidity and death rates of living things due to increased
levels of beta-lactamases. Plant-derived nanoparticles have gained a great
significance in the field of science and technology to combat bacterial diseases,
especially multidrug-resistant bacteria. This study examines the multidrug
resistance and virulent genes of identified pathogenic Staphylococcus species
obtained from Molecular Biotechnology and Bioinformatics Laboratory (MBBL), culture
collection. The polymerase chain reaction-based characterization of Staphylococcus
aureus and Staphylococcus argenteus having ON875315.1 and ON876003.1 accession IDs
revealed the presence of the spa, LukD, fmhA, and hld genes. The green synthesis of
silver nanoparticles (AgNPs) was carried out by utilizing the leaf extract of
Calliandra harrisii, of which metabolites act as capping and reducing agents for
the precursor of nano-synthesis, i.e., AgNO3 of 0.25 M. The synthesized AgNPs were
characterized via UV-vis spectroscopy, Fourier transform infrared spectroscopy,
scanning electron microscopy, and energy-dispersive X-ray analysis which inferred
the bead-like shape of our nanoparticles with the size of 2.21 nm with the
existence of aromatic and hydroxyl functional groups at surface plasmon resonance
of 477 nm. The antimicrobial activity by AgNPs showed 20 mm inhibition of
Staphylococcus species as compared to the vancomycin and cefoxitin antibiotics
along with crude plant extract, which showed a minimum zone of inhibition. The
synthesized AgNPs were also analyzed for various biological activities like anti-
inflammatory with 99.15% inhibition in protein denaturation, antioxidant with 99.8%
inhibition in free radical scavenging, antidiabetic with 90.56% inhibition of alpha
amylase assay, and anti-haemolytic with 89.9% inhibition in cell lysis which shows
good bioavailability and biocompatibility of the nanoparticles with the biological
system of the living being. The amplified genes (spa, LukD, fmhA, and hld) were
also analyzed for their interaction with AgNPs computationally at the molecular
level. The 3-D structure of AgNP and amplified genes was retrieved from ChemSpider
(ID: 22394) and Phyre2 online server, respectively. The binding affinities of AgNP
with spa, LukD, fmhA, and hld were −7.16, −6.5, −6.45, and −3.3 kJ/mol,
respectively, which infers a good docking score except of hld which is −3.3 kJ/mol
due to its small size. The salient features of biosynthesized AgNPs proved to be an
effective approach in combating the multidrug-resistant Staphylococcus species in
the future. © 2023 The Authors. Published by American Chemical Society.
AU - Waseem, M.
AU - Naveed, M.
AU - Rehman, S. U.
AU - Makhdoom, S. I.
AU - Aziz, T.
AU - Alharbi, M.
AU - Alsahammari, A.
AU - Alasmari, A. F.
DB - Scopus
DO - 10.1021/acsomega.3c01597
IS - 23
M3 - Article
PY - 2023
SP - 20920-20936
ST - Molecular Characterization of spa, hld, fmhA, and lukD Genes and
Computational Modeling the Multidrug Resistance of Staphylococcus Species through
Callindra harrisii Silver Nanoparticles
T2 - ACS Omega
TI - Molecular Characterization of spa, hld, fmhA, and lukD Genes and
Computational Modeling the Multidrug Resistance of Staphylococcus Species through
Callindra harrisii Silver Nanoparticles
85163361322&doi=10.1021%2facsomega.3c01597&partnerID=40&md5=3f37d13aeedeef75bbb101d
e571a90e2
VL - 8
ID - 4963
ER -
TY - JOUR
AB - Objectives. Previous studies have shown that biomaterials can activate
macrophages to produce cytokines and promote an inflammatory response. Although the
toxicity of many metal ions has been extensively investigated, little is known
about the ability of these ions to alter cytokine release from macrophages. Yet the
release of these ions from biomaterials has been well documented. Previous studies
indicated that alterations in cytokine release might be expected because metal ions
alter protein production in macrophages at sub-toxic concentrations. Thus, the
hypothesis of this study was that metal ions can alter the secretion of cytokines
from macrophages at sub-toxic concentrations. Methods. The release of interleukin-
1β (IL-1β) and tumor necrosis factor-α (TNF-α) from macrophages was investigated
when the macrophages were exposed to metal ions, with or without lipopolysaccharide
(LPS), a component of dental plaque. Human THP-1 macrophages were exposed to ions
of Ag, Au, Cu, Hg, and Ni for 24 h. In half of the cultures, LPS was added for the
last 4 h. The release of IL-1β and TNF-α into the medium was measured using enzyme-
linked immunosorbent assays. ANOVA and Tukey multiple comparison intervals were
used to compare the various experimental conditions. Results. None of the metal
ions elevated the IL-1β or TNF-α levels after 24 h, but Ni ions significantly
elevated the IL-1β and TNF-α levels after 72 h. With LPS added, Ag, Cu, and Ni
significantly amplified the LPS-induced production of IL-1β but only Ni amplified
the TNF-α response. These alterations in cytokine response occurred with metal ion
concentrations which have been previously shown to be released from dental alloys
in vitro and in vivo. Significance. It appeared plausible that macrophage-cytokine
mediated inflammatory responses may be altered by the presence of some metal ions
in tissues, particularly Ni.
AU - Wataha, J. C.
AU - Ratanasathien, S.
AU - Hanks, C. T.
AU - Sun, Z.
DB - Scopus
DO - 10.1016/s0109-5641(96)80041-8
IS - 5-6
KW - Analysis of Variance
Copper
Gold
Humans
Interleukin-1
Ions
Lipopolysaccharides
Macrophages
Mercury
Metals
Nickel
Silver
Time Factors
copper
gold
interleukin 1
ion
lipopolysaccharide
mercury
metal
nickel
silver
article
biosynthesis
dose response
drug effect
human
macrophage
metabolism
time
M3 - Article
PY - 1996
SP - 322-327
ST - In vitro IL-1β and TNF-α release from THP-1 monocytes in response to metal
ions
T2 - Dental Materials
TI - In vitro IL-1β and TNF-α release from THP-1 monocytes in response to metal
ions
0030275655&doi=10.1016%2fs0109-5641%2896%2980041-
8&partnerID=40&md5=5e642fc6be10f4e9335429419c7202a0
VL - 12
ID - 5776
ER -
TY - JOUR
AB - Treatment with drugs from multiple classes induces vascular injury with
medial necrosis, hemorrhage, endothelial damage, and inflammation. Previous
research has suggested early events might be occurring well in advance of the full
lesions that appear forty-eight to seventy-two hours after dosing with SCH 351591,
a PDE IV inhibitor. This study was performed to study early events in detail. Rats
were dosed with 20 mg/kg of drug by gavage and sacrificed at times between fifteen
and 240 minutes after dosing. Tissues were collected for histopathological analysis
and gene expression studies. Serum was collected for biomarker analysis. The data
from biomarker analysis showed a three-part response with an early phase that was
maximal at fifteen to thirty minutes, a second phase from forty-five to 180
minutes, and the third phase that was starting to rise at four hours. The first
phase included increases in lymphocytes, serum histamine, and serum nitrite. The
second phase shows continued elevation of serum nitrite. The third phase was marked
by an increase in serum GRO/CINC-1. At fifteen minutes, histopathology showed
activation of mast cells, but not degranulation. Increases in endothelial
activation and perivascular inflammatory cells were first apparent at thirty
minutes and increased through 240 minutes.
AN - WOS:000286314800007
AU - Weaver, J. L.
AU - Zhang, J.
AU - Knapton, A.
AU - Miller, T.
AU - Espandiari, P.
AU - Smith, R.
AU - Gu, Y. Z.
AU - Snyder, R. D.
DA - AUG
DO - 10.1177/0192623310374331
IS - 5
PY - 2010
SN - 0192-6233
1533-1601
SP - 738-744
ST - Early Events in Vascular Injury in the Rat Induced by the Phosphodiesterase
IV Inhibitor SCH 351591
TI - Early Events in Vascular Injury in the Rat Induced by the Phosphodiesterase
IV Inhibitor SCH 351591
VL - 38
ID - 6788
ER -
TY - JOUR
AB - Green synthesis of silver nanoparticles (AgNPs) has been extensively studied
by using a variety of plant extracts for applications in biomedical sciences and
engineering. However, there are no reports on the synthesis of AgNPs by utilizing
the berry extract of Sea Buckthorn, which is a traditional Chinese medicine and
exhibits a wide spectrum of antioxidant, anti-inflammatory and anticancer
activities. In this paper, we report an easy and eco-friendly technique for the
preparation of AgNPs using the Sea Buckthorn berry extract under ultrasonic
radiation at ambient temperature and the evaluation of both biosynthesis parameters
and biological activities. The UV-visible spectrum and dynamic light scattering
(DLS) analysis showed that the size of AgNPs was sensitive to the biosynthesis
parameters, such as the pH of the extract, material proportion and reaction time,
offering a size-controlled synthetic method for AgNPs. The X-ray diffraction (XRD),
transmission electron microscopy (TEM), selected area electron diffraction (SAED)
and DLS studies showed that the AgNPs (pH 10.0; material proportion 1 : 1; 4 h) had
a face-centered cubic (fcc) structure and spherical shape with an average particle
size of 27.3 +/- 0.2 nm covered by anions, and existed in a monodispersed form with
a polydispersity index (PDI) of 0.213. The biosynthesized AgNPs showed potent
anticancer activity against human colorectal cancer (HCT116 and SW620), hepatoma
cancer (HepG2), breast cancer (MCF-7) and cervical cancer (HeLa) cell lines as well
as strong antioxidant activity. The IC50 values for these five cell lines were
8.77, 4.61, 14.59, 16.05 and 27.98 mu g mL(-1), respectively. However, the
biosynthesized AgNPs revealed poor inhibition activities for the growth of E. coli
and S. aureus. These results confirmed that the Sea Buckthorn could be a low-cost,
nontoxic and eco-friendly natural resource for the synthesis of AgNPs, which might
be useful for the development of new alternative antioxidant and anticancer agents
in biomedicine.
AN - WOS:000540929200020
AU - Wei, S. M.
AU - Wang, Y. H.
AU - Tang, Z. S.
AU - Hu, J. H.
AU - Su, R.
AU - Lin, J. J.
AU - Zhou, T.
AU - Guo, H.
AU - Wang, N.
AU - Xu, R. R.
DA - JUN 14
DO - 10.1039/d0nj01335h
IS - 22
PY - 2020
SN - 1144-0546
1369-9261
SP - 9304-9312
ST - A size-controlled green synthesis of silver nanoparticles by using the berry
extract of Sea Buckthorn and their biological activities
TI - A size-controlled green synthesis of silver nanoparticles by using the berry
extract of Sea Buckthorn and their biological activities
VL - 44
ID - 6232
ER -
TY - JOUR
AB - Biomaterial-induced infection and inadequate osteointegration are regarded as
two major reasons for the failure of implants. Herein, niobium
(Nb)/polyetherketoneketone (PEKK) composite (NPC) was prepared, and a gallium (Ga)-
Nb nanofiber surface on NPC (NPCG) was fabricated through sequential treatment with
sodium hydroxide and gallium nitrate solution. Compared with PEKK and NPC, NPCG
with nanofiber surface exhibited significant enhancements in surface properties
with higher hydrophilicity and roughness, etc. NPCG remarkably facilitated
multiplication and osteoblastic differentiation of bone mesenchymal stem cells in
vitro. Moreover, NPCG obviously promoted the RAW264.7 cells polarization to M2
macrophages, which secreted anti-inflammatory cytokines in vitro, demonstrating an
anti-inflammatory effect. Furthermore, NPCG significantly boosted osteogenesis and
ameliorated osseointegration in vivo. The enhancements of osteoblastic response and
M2 macrophage polarization in vitro and osseointegration in vivo for NPCG were
attributed to the Ga-Nb nanofiber surface. Further, NPCG displayed outstanding
bactericidal capability, which not only inhibited the bacteria growth in vitro but
also resisted infection in vivo owing to the sustained release of Ga ions from the
nanofiber surface. In short, NPCG with good biocompatibility exhibited anti-
inflammatory, bactericidal and osteogenic capabilities, which facilitated
osteoblastic differentiation and M2 macrophage polarization, and ameliorated
osseointegration, thereby revealing great potential for bone substitute in
orthopedics.
AN - WOS:000970769500001
AU - Wei, W.
AU - Yang, R. X.
AU - Yu, Q.
AU - Zhao, J.
AU - Li, W. Z.
C6 - NOV 2022
C7 - 110375
DA - JAN 1
DO - 10.1016/j.compositesb.2022.110375
PY - 2023
SN - 1359-8368
1879-1069
ST - Gallium-niobium nanofiber surface of niobium/PEKK composite with anti-
inflammatory, osteogenesis and anti-bacterial effects for facilitating osteoblastic
differentiation and ameliorating osteointegration
T2 - COMPOSITES PART B-ENGINEERING
TI - Gallium-niobium nanofiber surface of niobium/PEKK composite with anti-
inflammatory, osteogenesis and anti-bacterial effects for facilitating osteoblastic
differentiation and ameliorating osteointegration
VL - 248
ID - 6084
ER -
TY - JOUR
AB - In traditional blood-contacting medical devices, infection and thrombosis are
easily formed on the surface of the materials. In addition, inflammation is also a
clinical complication that cannot be ignored. More importantly, there is a mutually
promoting relationship between the inflammatory response and the infection as well
as thrombosis. In this work, we propose a self-adaptive anti-inflammatory coating
strategy combined with anti-infection and anticoagulant capacity, which was
accomplished based on nano-Ag particles and dexamethasone (Dex)-loaded hydrogel
coating. The coating loaded with nano-Ag endows it with good bactericidal
performance, including Gram-positive and Gram-negative bacteria. As an anti-
inflammatory drug, Dex was grafted onto hydrogel coating by a reactive oxygen
species (ROS)-cleavable thioketal (TK) bond and released upon the trigger of an
inflammatory environment, blocking further inflammatory cascade, providing self-
adaptive anti-inflammatory properties, and avoiding side effects of the drug. It
was demonstrated that the coating worked as a precise strategy to resist
coagulation, infection, and inflammation, provided a new perspective for designing
clinical complication-conformable coatings, and had great application prospects on
blood-contacting medical devices. © 2022 American Chemical Society. All rights
reserved.
AU - Wei, Y.
AU - Liu, J.
AU - Liu, G.
AU - Gao, S.
AU - Wu, D.
AU - Yang, L.
AU - Luo, R.
AU - Zhang, F.
AU - Wang, Y.
DB - Scopus
DO - 10.1021/acs.biomac.2c00815
IS - 10
Anticoagulants
Dexamethasone
Humans
Hydrogels
Inflammation
Surface Properties
Thrombosis
Bacteria
Biofilms
Blood
Diseases
Pathology
dexamethasone
hydrogel
interleukin 1beta
silver nanoparticle
anticoagulant agent
antiinfective agent
Anti-infection
Anti-inflammatories
Clinical complications
Contacting devices
Dexamethasones
Haemocompatibility
Hydrogel coatings
Medical Devices
Property
aqueous solution
Article
bacterial viability
biocompatibility
blood compatibility
body weight gain
circulation
coating thickness
contact angle
controlled study
drug coating
erythrocyte adhesiveness
Escherichia coli
human
in vitro study
inflammation
macrophage
nonhuman
polymerization
RAW 264.7 cell line
chemistry
pharmacology
surface property
thrombosis
Coatings
M3 - Article
PY - 2022
SP - 4357-4369
ST - Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered
Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device
T2 - Biomacromolecules
TI - Hemocompatibility Multi-in-One Hydrogel Coating with ROS-Triggered
Inflammation Suppression and Anti-Infection Properties for Blood-Contacting Device
85139265106&doi=10.1021%2facs.biomac.2c00815&partnerID=40&md5=abd01d358ef1aaeb10d7d
f3e8dcaf99d
VL - 23
ID - 5067
ER -
TY - JOUR
AB - Acellular matrices are mainly composed of mammalian tissues, and aquatic
tissues with lower biological risks and less religious restrictions are considered
alternatives to mammalian tissues. The acellular fish skin matrix (AFSM) has been
commercially available. Silver carp has the advantages of farmability, high yield
and low price, but there are few studies on the silver carp acellular fish skin
matrix (SC-AFSM). In this study, an acellular matrix with low DNA and endotoxin was
prepared from the skin of silver carp. After treatment with trypsin/sodium dodecyl
sulfate and Triton X-100 solutions, the DNA content in SC-AFSM reached 11.03 ± 0.85
ng/mg, and the endotoxin removal rate was 96.8%. The porosity of SC-AFSM was 79.64%
± 0.17%, which is favorable for cell infiltration and proliferation. The relative
cell proliferation rate of SC-AFSM extract was 117.79% ± 15.26%. The wound healing
experiment showed that SC-AFSM had no adverse acute pro-inflammatory response,
which had a similar effect as commercial products in promoting tissue repair.
Therefore, SC-AFSM has great application potential in biomaterials. © 2023 Wiley
Periodicals LLC.
AU - Wei, Z.
AU - Zhang, J.
AU - Guo, Z.
AU - Wu, Z.
AU - Sun, Y.
AU - Wang, K.
AU - Duan, R.
DB - Scopus
DO - 10.1002/jbm.b.35236
IS - 6
KW - acellular matrix
biomaterials
DNA content
endotoxin
silver carp skin
Animals
Carps
Endotoxins
Mammals
Cell proliferation
DNA
Histology
Silver
Sulfur compounds
Tissue
triton x 100
trypsin
Acellular matrices
Biological risks
Endotoxin
Higher yield
Mammalian tissues
Property
Silver carp
Silver carp skin
animal experiment
animal tissue
Article
biocompatibility
cell infiltration
cell proliferation
controlled study
cytotoxicity test
decellularization
male
mouse
nonhuman
porosity
tissue repair
wound healing
wound healing assay
animal
carp
mammal
Fish
M3 - Article
PY - 2023
SP - 1328-1335
ST - Study on the preparation and properties of acellular matrix from the skin of
silver carp (Hypophthalmichthys molitrix)
TI - Study on the preparation and properties of acellular matrix from the skin of
silver carp (Hypophthalmichthys molitrix)
85148608241&doi=10.1002%2fjbm.b.35236&partnerID=40&md5=7c37332862ba9323580738ec4a68
154d
VL - 111
ID - 4973
ER -
TY - JOUR
AB - Biomedical device-associated infections (BAI) and osteosynthesis are two main
complications following the orthopedic implant surgery, especially while infecting
bacteria form a mature biofilm, which can protect the organisms from the host
immune system and antibiotic therapy. Comparing with the single antibiotics
therapeutic method, the combination of silver nanoparticles (AgNPs) and
conventional antibiotics exert a high level of antibacterial activity.
Nevertheless, one major issue that extremely restricts the potential application of
AgNP/antiviotics is the uncontrolled release. Moreover, the lack of osteogenic
ability may cause the osteosynthesis. Thus, herein we fabricated a structure-
controlled drug-loaded silk fibroin (SF) coating that can achieve the size and
release control of AgNPs and high efficient osteogenesis. Three comparative SF-
based coatings were fabricated: alpha-structured coating (alpha-helices 32.7%,), m-
structured coating (beta-sheets 28.3%) and beta-structured coating (beta-sheets
41%). Owning to the high content of alpha-helices structure and small AgNPs (20
nm), alpha-structured coating displayed better protein adsorption and
hydrophilicity, as well as pH-dependent and long-lasting antibacterial performance.
In vitro studies demonstrated that alpha coating showed biocompatibility (cellular
attachment, spreading and proliferation), high ALP expression, collagen secretion
and calcium mineralization. Moreover, after one month subcutaneous implantation in
vivo, alpha-structured coating elicited minimal, comparable inflammatory response.
Additionally, in a rabbit femoral defect model, alpha-structured coating displayed
a significant improvement on the generation of new-born bone and bonding between
the new bone and the tissue, implying a rapid and durable osteointegration.
Expectedly, this optimized structure-controlled SF-based coating can be an
alternative and prospective solution for the current challenges in orthopedics.
Statement of Significance In this study, an AgNPs/Gentamycin-loaded structured-
controlled silk fibroin coatings were constructed on Ti implant's surface to
guarantee the success of implantation even in the face of bacterial infection. In
comparison, the alpha-structured coating had the lowest content of beta-sheets
structure (19.0%) and the smallest particle size of AgNPs (similar to 20 nm), and
owned pH-responsive characteristic due to reversible alpha-helices structural.
Thanks to pH-responsive release of Ag+, the alpha-structure coating could
effectively inhibit adhesive bacteria and kill planktonic bacteria by releasing a
large amount of reactive oxygen radicals. Through in vitro biological results (cell
proliferation, differentiation and osteogenic gene expression) and in vivo rabbit
femur implantation results, the alpha-structure coating had good biocompatible and
osteogenic properties. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All
rights reserved.
AN - WOS:000577516000014
AU - Wenhao, Z.
AU - Zhang, T.
AU - Yan, J. L.
AU - Li, Q. Y.
AU - Xiong, P. P.
AU - Li, Y. Y.
AU - Cheng, Y.
AU - Zheng, Y. F.
DA - OCT 15
DO - 10.1016/j.actbio.2020.08.040
PY - 2020
SN - 1742-7061
1878-7568
SP - 223-245
ST - In vitro and in vivo evaluation of structurally-controlled silk fibroin
coatings for orthopedic infection and in-situ osteogenesis
TI - In vitro and in vivo evaluation of structurally-controlled silk fibroin
VL - 116
ID - 6631
ER -
TY - JOUR
AB - Biomedical device-associated infections (BAI) and osteosynthesis are two main
complications following the orthopedic implant surgery, especially while infecting
bacteria form a mature biofilm, which can protect the organisms from the host
immune system and antibiotic therapy. Comparing with the single antibiotics
therapeutic method, the combination of silver nanoparticles (AgNPs) and
conventional antibiotics exert a high level of antibacterial activity.
Nevertheless, one major issue that extremely restricts the potential application of
AgNP/antiviotics is the uncontrolled release. Moreover, the lack of osteogenic
ability may cause the osteosynthesis. Thus, herein we fabricated a structure-
controlled drug-loaded silk fibroin (SF) coating that can achieve the size and
release control of AgNPs and high efficient osteogenesis. Three comparative SF-
based coatings were fabricated: α-structured coating (α-helices 32.7%,), m-
structured coating (β-sheets 28.3%) and β-structured coating (β-sheets 41%). Owning
to the high content of α-helices structure and small AgNPs (20 nm), α-structured
coating displayed better protein adsorption and hydrophilicity, as well as pH-
dependent and long-lasting antibacterial performance. In vitro studies demonstrated
that α coating showed biocompatibility (cellular attachment, spreading and
proliferation), high ALP expression, collagen secretion and calcium mineralization.
Moreover, after one month subcutaneous implantation in vivo, α-structured coating
elicited minimal, comparable inflammatory response. Additionally, in a rabbit
femoral defect model, α-structured coating displayed a significant improvement on
the generation of new-born bone and bonding between the new bone and the tissue,
implying a rapid and durable osteointegration. Expectedly, this optimized
structure-controlled SF-based coating can be an alternative and prospective
solution for the current challenges in orthopedics. Statement of Significance: In
this study, an AgNPs/Gentamycin-loaded structured-controlled silk fibroin coatings
were constructed on Ti implant's surface to guarantee the success of implantation
even in the face of bacterial infection. In comparison, the α-structured coating
had the lowest content of β-sheets structure (19.0%) and the smallest particle size
of AgNPs (~ 20 nm), and owned pH-responsive characteristic due to reversible α-
helices structural. Thanks to pH-responsive release of Ag+, the α-structure coating
could effectively inhibit adhesive bacteria and kill planktonic bacteria by
releasing a large amount of reactive oxygen radicals. Through in vitro biological
results (cell proliferation, differentiation and osteogenic gene expression) and in
vivo rabbit femur implantation results, the α-structure coating had good
biocompatible and osteogenic properties. © 2020
AU - Wenhao, Z.
AU - Zhang, T.
AU - Yan, J.
AU - Li, Q.
AU - Xiong, P.
AU - Li, Y.
AU - Cheng, Y.
AU - Zheng, Y.
DB - Scopus
DO - 10.1016/j.actbio.2020.08.040
KW - AgNPs, β-sheets structure
Antibacterial
Osteogenesis
Silk fibroin
Animals
Fibroins
Metal Nanoparticles
Orthopedics
Prospective Studies
Rabbits
Silver
Antibiotics
Bacteria
Biocompatibility
Particle size
collagen
fluorescent dye
silk fibroin
silver nanoparticle
streptomycin
antiinfective agent
fibroin
metal nanoparticle
silver
AgNP, β-sheet structure
Antibacterials
In-vitro
In-vivo
Osteogenic
Osteosynthesis
PH-responsive
β-sheet structure
animal cell
animal experiment
animal model
antibiotic therapy
Article
beta sheet
biocompatibility
bone development
bone infection
bone mineralization
cell proliferation
comparative study
controlled study
gene expression
hydrophilicity
in vitro study
in vivo study
inflammation
MC3T3-E1 cell line
micro-computed tomography
mouse
nanofabrication
nonhuman
oxidative stress
priority journal
protein expression
wettability
animal
Leporidae
orthopedics
prospective study
Coatings
M3 - Article
PY - 2020
SP - 223-245
ST - In vitro and in vivo evaluation of structurally-controlled silk fibroin
TI - In vitro and in vivo evaluation of structurally-controlled silk fibroin
85090987513&doi=10.1016%2fj.actbio.2020.08.040&partnerID=40&md5=4815452c37668fa561c
5b7cd86c77629
VL - 116
ID - 5301
ER -
TY - JOUR
AB - Suppression of angiotensin II formation by angiotensin-converting enzyme
inhibitors or blockade of the angiotensin II receptor by angiotensin receptor
blockers is a powerful therapeutic strategy to slow the progression of renal
disease. However, angiotensin-converting enzyme inhibitors and angiotensin receptor
blockers provide only imperfect protection against the progression of chronic
kidney disease to end-stage renal failure. Hence, innovative approaches are needed
to keep patients with chronic kidney disease off dialysis. Angiotensin II activates
at least two receptors, namely the angiotensin II type 1 (AT1) and angiotensin II
type 2 (AT2) receptors. The majority of the effects of angiotensin II, such as
vasoconstriction, inflammation, and matrix deposition, are mediated via the AT1
receptor. It is thought that the AT2 receptor counteracts these effects and plays a
role in nephroprotection. However, recent data support the notion that the AT2
receptor transduces pro-inflammatory effects and promotes fibrosis and hypertrophy.
Therefore, the question of whether stimulation of the AT2 receptor could represent
a silver bullet for the treatment of chronic kidney disease or may, on the
contrary, exert detrimental effects on renal physiology remains unresolved. Recent
data from AT2 receptor-knockout mice demonstrate that the loss of AT 2 receptor
signalling is associated with increased renal injury and mortality in chronic
kidney disease. This raises the expectation that pharmacological stimulation of the
AT2 receptor may positively influence renal pathologies. However, further research
is needed to explore the question whether AT2 receptor stimulation may represent a
new therapeutic strategy for the treatment of chronic kidney disease.
AU - Wenzel, U. O.
AU - Krebs, C.
AU - Benndorf, R.
DB - Scopus
DO - 10.1177/1470320309347787
IS - 1
KW - Angiotensin
AT2 receptor
Fibrosis
Inflammation
Kidney
Angiotensin II Type 1 Receptor Blockers
Animals
Humans
Kidney Diseases
Kidney Failure, Chronic
Receptor, Angiotensin, Type 1
Receptor, Angiotensin, Type 2
angiotensin 1 receptor
angiotensin 2 receptor
angiotensin receptor antagonist
aldosterone release
apoptosis
blood pressure regulation
cell growth
chronic kidney disease
disease course
fibrosis
hormone action
human
hypertrophy
kidney development
knockout mouse
negative feedback
nonhuman
pathogenesis
pathophysiology
pharmacodynamics
renal protection
review
sodium transport
vasoconstriction
M3 - Review
PY - 2010
SP - 37-41
ST - The angiotensin II type 2 receptor in renal disease
T2 - JRAAS - Journal of the Renin-Angiotensin-Aldosterone System
TI - The angiotensin II type 2 receptor in renal disease
77749254986&doi=10.1177%2f1470320309347787&partnerID=40&md5=68a6bc0dc46fc49c4348449
2c58f99eb
VL - 11
ID - 5746
ER -
TY - JOUR
AB - Nanocrystalline silver (NCS) has proven to be an important wound dressing
particularly in chronic infected wounds. However, debate still rages around its use
in the case of partially epithelialized wounds, particularly when these are non-
infected. Much of the debate has revolved around seemingly contradictory research
publications that blurred the use of NCS in these clinical situations, primarily
based on reported cytotoxic effects of NCS on cell lines in vitro. MMPs, in
particular MMP-9 (gelatinase) has been demonstrated to be pivotal in the
progression from keratinocyte cleavage, to migration and re-epithelialisation. High
levels promote increases in TNF-α; IL-8 and TGFβ, all associated with exaggerated
ongoing inflammation and chronicity. Low levels impede the process of keratinocyte
migration. Thus, as in so many clinical situations, a balance of MMP level is
extremely important. NCS has been demonstrated to decrease these undesirable high
levels of MMP-9 making it an ideal dressing for chronic infected wounds, acute
inflamed wounds and burn wounds of all types which are associated with protracted
raised MMP-9 levels. The converse applies too - NCS used in a situation of minimal
inflammation may undesirably decrease the low levels of MMP-9 and adversely affect
epithelialisation. NCS would be contra-indicated in conjunction with cell lines in
vitro, cell cultured lines in vivo and integrated artificial matrices with added
cell lines. Therapeutic decisions for different clinical situations may thus be
made more predictably. © 2010 Elsevier Ltd and ISBI. All rights reserved.
AU - Widgerow, A. D.
DB - Scopus
DO - 10.1016/j.burns.2010.01.010
IS - 7
KW - Burns
Cytotoxicity
Gelatinases
MMP-9
Animals
Bandages
Cytotoxins
Honey
Humans
Nanoparticles
Silver
Skin
Swine
Wound Healing
Wound Infection
Wounds and Injuries
gelatinase A
gelatinase B
interleukin 8
petrolatum
silver
silver nitrate
steroid
tacrolimus
unclassified drug
apoptosis
burn
cell culture
cell line
cell migration
cell proliferation
cell viability
cytotoxicity
edema
epithelization
erythema
explant
fibroblast
honey
human
inflammation
keratinocyte
monolayer culture
nonhuman
review
skin allergy
wound care
wound dressing
wound healing
wound infection
M3 - Review
PY - 2010
SP - 965-974
ST - Nanocrystalline silver, gelatinases and the clinical implications
T2 - Burns
TI - Nanocrystalline silver, gelatinases and the clinical implications
77957589985&doi=10.1016%2fj.burns.2010.01.010&partnerID=40&md5=7a3f886d874a9578d2ef
82287541dd9b
VL - 36
ID - 5708
ER -
TY - CPAPER
AB - Chronic wounds, such as venous, pressure, and diabetic ulcers, are difficult
to heal and represent a rising social and economical problem. Compared to acute
wounds, non-healing wounds contain elevated levels of neutrophil elastase, pro-
inflammatory cytokines (IL-1 beta, IL-6, IL-8), and matrix metalloproteases (MMP-2,
MMP-9, MMP-13) as well as free radicals. Their overproduction perpetuates the
inflammatory phase resulting in severe tissue damage and degradation of growth
factors. Consequently, wound closure is prevented and the wound remains non-healing
for month or even years. The increasing numbers of patients suffering from wounds
that fail to heal are a significant challenge for health care professionals. Wound
dressings play an important role in the entire management of these wounds. New
materials and treatment strategies are needed to improve wound care. Recent
advances in the field of biomaterials and their medical applications indicate the
significance and potential of various natural polymers in the development of novel
classes of wound dressings. Native polymers are an ideal source for bio-active
wound dressings because of their availability and biocompatibility. Hence, several
studies have been conducted to explore the influence of wound dressings consisting
of collagen, oxidized regenerated cellulose, bacterial cellulose, chitosan, or
alginate on the destructive milieu in chronic wounds.
AN - WOS:000282841600001
AU - Wiegand, C.
AU - Hipler, U. C.
DO - 10.1002/masy.200900028
PY - 2010
SP - 1-13
T2 - UTILIZATION OF LIGNOCELLULOSIC MATERIALS
TI - Polymer-based Biomaterials as Dressings for Chronic Stagnating Wounds
VL - 294-II
ID - 6782
ER -
TY - JOUR
AB - The distribution of silver (Ag) into remote organs secondary to the
application of Ag nanoparticles (Ag-NP) to the lung is still incompletely
understood and was investigated in the rat with imaging methods. Dose-finding
experiments were carried out with 50 nm- or 200 nm-sized polyvinyl pyrrolidine
(PVP)-coated Ag-NP using alveolar macrophages in vitro and female rats, which
received Ag-NP via intratracheal instillation. In the main study, we administered
37.5–300 µg per rat lung of the more toxic Ag50-PVP and assessed the broncho-
alveolar lavage fluid (BALF) for inflammatory cells, total protein and fibronectin
after three and 21 days. In parallel, lung tissue was analysed for DNA double-
strand breaks and altered cell proliferation. While 75–150 µg Ag50-PVP per rat lung
caused a reversible inflammation, 300 µg led to DNA damage, accelerated cell
proliferation and progressively increasing numbers of neutrophilic granulocytes. Ag
accumulation was significant in homogenates of liver and other peripheral organs
upon lung dose of ≥75 µg. Quantitative laser-ablation inductively-coupled plasma
mass spectrometry (LA-ICP-MS) combined with enhanced dark field microscopy and
autometallography revealed focal accumulations of Ag and/or Ag-NP in sections of
peripheral organs: mediastinal lymph nodes contained Ag-NP especially in peripheral
macrophages and Ag in argyrophilic fibres. In the kidney, Ag had accumulated within
proximal tubuli, while renal filter structures contained no Ag. Discrete
localizations were also observed in immune cells of liver and spleen. Overall, the
study shows that concentrations of Ag-NP, which elicit a transient inflammation in
the rat lung, lead to focal accumulations of Ag in peripheral organs, and this
might pose a risk to particular cell populations in remote sites. © 2017 by the
AU - Wiemann, M.
AU - Vennemann, A.
AU - Blaske, F.
AU - Sperling, M.
AU - Karst, U.
C7 - 441
DB - Scopus
DO - 10.3390/nano7120441
IS - 12
KW - In vitro toxicity
Nanoparticle transition
Quantitative bio-imaging
Silver nanoparticle
M3 - Article
PY - 2017
ST - Silver nanoparticles in the lung: Toxic effects and focal accumulation of
silver in remote organs
T2 - Nanomaterials
TI - Silver nanoparticles in the lung: Toxic effects and focal accumulation of
silver in remote organs
85038578908&doi=10.3390%2fnano7120441&partnerID=40&md5=dfec9298fa5c41bc31bc79ef5865
8859
VL - 7
ID - 5487
ER -
TY - JOUR
AB - Antibacterial surfaces coated with nanomaterials, including silver
nanoparticles, are considered effective alternative antimicrobial agents that can
be used instead of antibiotics and chemical agents. However, reports of the
potential toxicity of these materials raise questions about the safety of their use
in biomedical applications. The objective of this research was to reduce the human
cell cytotoxicity of silver nanoparticle-coated polyurethane foils by complexing
silver nanoparticles with graphene oxide. The antimicrobial activity of
nanoplatforms coated with silver nanoparticles, graphene oxide and the composite of
silver nanoparticles and graphene oxide was assessed with Salmonella enteritidis.
Cytotoxicity was analysed by an analysis of the viability and morphology of human
fibroblasts, human umbilical vein endothelial cells (HUVECs) and chicken embryo
chorioallantoic membrane. Additionally, the synthesis level of inflammatory
proteins was examined for fibroblasts cultured on different nanoplatforms. The
nanoplatform coated with the silver nanoparticles and graphene oxide composite
showed strongest antibacterial properties, although nanoplatforms coated with only
silver nanoparticles or graphene oxide also resulted in decreased S. enteritidis
growth. Furthermore, a nanoplatform coated with silver nanoparticles and graphene
oxide composite showed limited immunological stimulation and significantly reduced
cytotoxicity towards fibroblasts, HUVECs and chicken embryo chorioallantoic
membrane in comparison to the nanoplatform coated only with silver nanoparticles,
due to the higher stability of the nanomaterials in the nanocomposite.
AN - WOS:000504277700001
AU - Wierzbicki, M.
AU - Jaworski, S.
AU - Sawosz, E.
AU - Jung, A.
AU - Gielerak, G.
AU - Jaremek, H.
AU - Lojkowski, W.
AU - Wozniak, B.
AU - Stobinski, L.
AU - Malolepszy, A.
AU - Chwalibog, A.
C7 - 320
DA - OCT 11
DO - 10.1186/s11671-019-3166-9
IS - 1
PY - 2019
SN - 1931-7573
1556-276X
ST - Graphene Oxide in a Composite with Silver Nanoparticles Reduces the
Fibroblast and Endothelial Cell Cytotoxicity of an Antibacterial Nanoplatform
TI - Graphene Oxide in a Composite with Silver Nanoparticles Reduces the
VL - 14
ID - 5846
ER -
TY - JOUR
AB - Antibacterial surfaces coated with nanomaterials, including silver
nanoparticles, are considered effective alternative antimicrobial agents that can
be used instead of antibiotics and chemical agents. However, reports of the
potential toxicity of these materials raise questions about the safety of their use
in biomedical applications. The objective of this research was to reduce the human
cell cytotoxicity of silver nanoparticle-coated polyurethane foils by complexing
silver nanoparticles with graphene oxide. The antimicrobial activity of
nanoplatforms coated with silver nanoparticles, graphene oxide and the composite of
silver nanoparticles and graphene oxide was assessed with Salmonella enteritidis.
Cytotoxicity was analysed by an analysis of the viability and morphology of human
fibroblasts, human umbilical vein endothelial cells (HUVECs) and chicken embryo
chorioallantoic membrane. Additionally, the synthesis level of inflammatory
proteins was examined for fibroblasts cultured on different nanoplatforms. The
nanoplatform coated with the silver nanoparticles and graphene oxide composite
showed strongest antibacterial properties, although nanoplatforms coated with only
silver nanoparticles or graphene oxide also resulted in decreased S. enteritidis
growth. Furthermore, a nanoplatform coated with silver nanoparticles and graphene
oxide composite showed limited immunological stimulation and significantly reduced
cytotoxicity towards fibroblasts, HUVECs and chicken embryo chorioallantoic
membrane in comparison to the nanoplatform coated only with silver nanoparticles,
due to the higher stability of the nanomaterials in the nanocomposite. © 2019, The
Author(s).
AU - Wierzbicki, M.
AU - Jaworski, S.
AU - Sawosz, E.
AU - Jung, A.
AU - Gielerak, G.
AU - Jaremek, H.
AU - Łojkowski, W.
AU - Woźniak, B.
AU - Stobiński, L.
AU - Małolepszy, A.
AU - Chwalibog, A.
C7 - 320
DB - Scopus
DO - 10.1186/s11671-019-3166-9
IS - 1
KW - Antibacterial surface
Endothelial cells
Fibroblasts
Graphene oxide
Toxicity
Animals
Biosynthesis
Cell culture
Cytotoxicity
Graphene
Metal nanoparticles
Morphology
Salmonella
Antibacterial surfaces
Chorio-allantoic membranes
Immunological stimulation
Salmonella enteritidis
M3 - Article
PY - 2019
ST - Graphene Oxide in a Composite with Silver Nanoparticles Reduces the
TI - Graphene Oxide in a Composite with Silver Nanoparticles Reduces the
85073555009&doi=10.1186%2fs11671-019-3166-
9&partnerID=40&md5=6f47c3c3c402bb53816424d9273b1f7d
VL - 14
ID - 5447
ER -
TY - JOUR
AB - Pancreatic cancer, due to its asymptomatic development and drug-resistance,
is difficult to cure. As many metallic and carbon-based nanomaterials have shown
anticancer properties, we decided to investigate their potential use as anticancer
agents against human pancreatic adenocarcinoma. The objective of the study was to
evaluate the toxic properties of the following nanomaterials: silver (Ag), gold
(Au), platinum (Pt), graphene oxide (GO), diamond (ND), and fullerenol (C-60(OH)
(40)) against the cell lines BxPC-3, AsPC-1, HFFF-2, and HS-5. The potential
cytotoxic properties were evaluated by the assessment of the cell morphology, cell
viability, and cell membrane damage. The cancer cell responses to GO and ND were
analysed by determination of changes in the levels of 40 different pro-inflammatory
proteins. Our studies revealed that the highest cytotoxicity was obtained after the
ND treatment. Moreover, BxPC-3 cells were more sensitive to ND than AsPC-1 cells
due to the ND-induced ROS production. Furthermore, in both of the cancer cell
lines, ND caused an increased level of IL-8 and a decreased level of TIMP-2,
whereas GO caused only decreased levels of TIMP-2 and ICAM-1 proteins. This work
provides important data on the toxicity of various nanoparticles against pancreatic
adenocarcinoma cell lines.
AN - WOS:000728592800001
AU - Wojcik, B.
AU - Sawosz, E.
AU - Szczepaniak, J.
AU - Strojny, B.
AU - Sosnowska, M.
AU - Daniluk, K.
AU - Zielinska-Gorska, M.
AU - Balaban, J.
AU - Chwalibog, A.
AU - Wierzbicki, M.
C7 - 12100
DA - NOV
DO - 10.3390/ijms222212100
IS - 22
PY - 2021
SN - 1422-0067
ST - Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer
Cell Lines AsPC-1 and BxPC-3
TI - Effects of Metallic and Carbon-Based Nanomaterials on Human Pancreatic Cancer
Cell Lines AsPC-1 and BxPC-3
VL - 22
ID - 6421
ER -
TY - JOUR
AB - Pancreatic cancer, due to its asymptomatic development and drug-resistance,
is difficult to cure. As many metallic and carbon-based nanomaterials have shown
anticancer properties, we decided to investigate their potential use as anticancer
agents against human pancreatic adenocarcinoma. The objective of the study was to
evaluate the toxic properties of the following nanomaterials: silver (Ag), gold
(Au), platinum (Pt), graphene oxide (GO), diamond (ND), and fullerenol (C60
(OH)40 ) against the cell lines BxPC-3, AsPC-1, HFFF-2, and HS-5. The potential
cytotoxic properties were evaluated by the assessment of the cell morphology, cell
viability, and cell membrane damage. The cancer cell responses to GO and ND were
analysed by determination of changes in the levels of 40 different pro-inflammatory
proteins. Our studies revealed that the highest cytotoxicity was obtained after the
ND treatment. Moreover, BxPC-3 cells were more sensitive to ND than AsPC-1 cells
due to the ND-induced ROS production. Furthermore, in both of the cancer cell
lines, ND caused an increased level of IL-8 and a decreased level of TIMP-2,
whereas GO caused only decreased levels of TIMP-2 and ICAM-1 proteins. This work
provides important data on the toxicity of various nanoparticles against pancreatic
adenocarcinoma cell lines. © 2021 by the authors. Licensee MDPI, Basel,
Switzerland.
AU - Wójcik, B.
AU - Sawosz, E.
AU - Szczepaniak, J.
AU - Strojny, B.
AU - Sosnowska, M.
AU - Daniluk, K.
AU - Zielińska-Górska, M.
AU - Bałaban, J.
AU - Chwalibog, A.
AU - Wierzbicki, M.
C7 - 12100
DB - Scopus
DO - 10.3390/ijms222212100
IS - 22
KW - Carbon-based nanomaterials
Cytotoxicity
In vitro
Pancreatic cancer
carbon nanoparticle
colony stimulating factor 1
CXCL9 chemokine
cytotoxic agent
eotaxin
eotaxin 2
fullerenol nanoparticle
gamma interferon
gold nanoparticle
graphene oxide
graphene oxide nanoparticle
interleukin 10
interleukin 11
interleukin 12p40
interleukin 12p70
interleukin 13
interleukin 15
interleukin 16
interleukin 17
interleukin 1alpha
interleukin 1beta
interleukin 2
interleukin 3
interleukin 4
interleukin 6
interleukin 7
interleukin 8
lymphotoxin
macrophage inflammatory protein 1delta
metal nanoparticle
nanodiamond
platelet derived growth factor BB
RANTES
silver nanoparticle
unclassified drug
Article
AsPC-1 cell line
BxPC-3 cell line
cell membrane
cell structure
cell viability
controlled study
cytokine production
drug cytotoxicity
HFFF2 cell line
HS-5 cell line
human
human cell
hydrodynamics
membrane damage
particle size
physical chemistry
zeta potential
M3 - Article
PY - 2021
ST - Effects of metallic and carbon-based nanomaterials on human pancreatic cancer
cell lines aspc-1 and bxpc-3
TI - Effects of metallic and carbon-based nanomaterials on human pancreatic cancer
cell lines aspc-1 and bxpc-3
85118652438&doi=10.3390%2fijms222212100&partnerID=40&md5=9486e9f3d48c556382c118d0ae
011db5
VL - 22
ID - 5249
ER -
TY - JOUR
AB - With the advent of nanoscience, pure silver can now be made into nanometer
sized particles. As a result, we are able to explore the potentially beneficial
properties of pure silver. In our previous study using a burn wound model in mice,
we demonstrated that besides antibacterial action, silver nanoparticles (nAg)
appear to have anti-inflammatory properties. Herein we further confirm the anti-
inflammatory effects of nAg and explore their potential clinical application
through a postoperative peritoneal adhesion model. We also elucidate the potential
mechanism of action of silver. Our in vitro and in vivo experimental findings. show
that nAg are effective at decreasing inflammation in peritoneal adhesions without
significant toxic effects: This study thus provides further evidence for and
contributes to the understanding of the anti-inflammatory properties of nAg and may
also give a novel therapeutic direction for the prevention of postoperative
adhesions.
AN - WOS:000267893000012
AU - Wong, K. K. Y.
AU - Cheung, S. O. F.
AU - Huang, L. M.
AU - Niu, J.
AU - Tao, C.
AU - Ho, C. M.
AU - Che, C. M.
AU - Tam, P. K. H.
DA - JUL
DO - 10.1002/cmdc.200900049
IS - 7
PY - 2009
SN - 1860-7179
1860-7187
SP - 1129-1135
ST - Further Evidence of the Anti-inflammatory Effects of Silver Nanoparticles
T2 - CHEMMEDCHEM
TI - Further Evidence of the Anti-inflammatory Effects of Silver Nanoparticles
VL - 4
ID - 5847
ER -
TY - JOUR
AB - With the advent of nanoscience, pure silver can now be made into nanometer-
sized particles. As a result, we are able to explore the potentially beneficial
properties of pure silver. In our previous study using a burn wound model in mice,
we demonstrated that besides antibacterial action, silver nanoparticles (nAg)
appear to have anti-inflammatory properties. Herein we further confirm the anti-
inflammatory effects of nAg and explore their potential clinical application
through a postoperative peritoneal adhesion model. We also elucidate the potential
mechanism of action of silver. Our in vitro and in vivo experimental findings show
that nAg are effective at decreasing inflammation in peritoneal adhesions without
significant toxic effects. This study thus provides further evidence for and
contributes to the understanding of the anti-inflammatory properties of nAg and may
also give a novel therapeutic direction for the prevention of postoperative
adhesions. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
AU - Wong, K. K. Y.
AU - Cheung, S. O. F.
AU - Huang, L.
AU - Niu, J.
AU - Tao, C.
AU - Ho, C. M.
AU - Che, C. M.
AU - Tam, P. K. H.
DB - Scopus
DO - 10.1002/cmdc.200900049
IS - 7
KW - Inflammation
Nanoparticles
Silver
Toxicity
Animals
Cell Line
Gold
Interferon-gamma
Lipopolysaccharides
Metal Nanoparticles
Mice
Mice, Inbred C57BL
Models, Animal
Peritoneal Diseases
gamma interferon
lipopolysaccharide
metal nanoparticle
silver nanoparticle
unclassified drug
animal cell
animal experiment
animal model
animal tissue
apoptosis
article
cell death
cell viability
controlled study
disease severity
drug efficacy
drug mechanism
drug safety
histopathology
inflammation
macrophage
mouse
nonhuman
peritoneum adhesion
postoperative period
priority journal
protein expression
protein synthesis
staining
M3 - Article
PY - 2009
SP - 1129-1135
ST - Further evidence of the anti-inflammatory effects of silver nanoparticles
T2 - ChemMedChem
TI - Further evidence of the anti-inflammatory effects of silver nanoparticles
67749142233&doi=10.1002%2fcmdc.200900049&partnerID=40&md5=85bff30b2f2db14bda8307dea
c9b9e06
VL - 4
ID - 5758
ER -
TY - JOUR
AB - To overcome the limitations of balloon expandible metal stent-induced
neointimal smooth muscle cell proliferation, drug-coated stent devices have been
developed. Drug eluting steins release high concentrations of antiproliferative
agents, such as paclitaxel, to reduce neointimal hyperplasia. The proinflammatory
cytokine, tumor necrosis factor-alpha (TNF-alpha), is known to cause severe
endothelial dysfunction and accelerate atherosclerotic lesion progression. The
interaction of TNF-alpha and paclitaxel on the release of prothrombotic molecules
was examined in endothelial cells. Treatment of endothelial cells with paclitaxel
had no direct effect on tissue factor (TF) expression, but TNF-alpha increased TF.
Cotreatment of paclitaxel with TNF-alpha markedly augmented the release of TF. TNF-
alpha induced release of plasminogen activator inhibitor but no synergism occurred
with paclitaxel. Treatment of endothelial cells with paclitaxel and TNF-alpha
reduced expression of thrombomodulin and protein C receptor. Tissue factor pathway
inhibitor expression was reduced by prolonged treatment with either paclitaxel or
TNF-alpha. The adhesion molecule, CD62 E, was induced by TNF-alpha; however, CD31,
CD62 P, and CD 106 were not affected by paclitaxel and TNF-alpha. Apoptosis was not
observed with cotreatment of endothelial cells with paclitaxel and TNF-alpha. CD59-
positive microparticles were released in response to TNF-alpha, but the release was
not augmented by paclitaxel. Paclitaxel and TNF-alpha increased the
nitrotyrosination of proteins. These findings indicate that paclitaxel enhances
TNF-alpha induced release of TF, and downregulated thrombomodulin, increased
protein nitration, which may subsequently favor prothrombotic intimal surface.
AN - WOS:000276135900009
AU - Wood, S. C.
AU - Tang, X.
DA - MAR
DO - 10.1097/FJC.0b013e3181d263f7
IS - 3
PY - 2010
SN - 0160-2446
SP - 276-285
ST - Paclitaxel Potentiates Inflammatory Cytokine-induced Prothrombotic Molecules
in Endothelial Cells
T2 - JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
TI - Paclitaxel Potentiates Inflammatory Cytokine-induced Prothrombotic Molecules
in Endothelial Cells
VL - 55
ID - 6321
ER -
TY - JOUR
AB - A porcine model of wound healing was employed to examine the impact of
nanocrystalline silver-coated dressings on specific wound healing events. Full-
thickness wounds were created on the backs of pigs, contaminated with an
experimental inoculum containing Pseudomonas aeruginosa. Fusobacterium sp., and
coagulase-negative staphylococci, and covered with dressing products either
containing silver or not. Nanocrystalline silver-coated dressings promoted rapid
wound healing, particularly during the first several days post-injury. Healing was
characterized by rapid development of well vascularized granulation tissue that
supported tissue grafting 4 days post-injury, unlike control dressed wounds. The
proteolytic environment of wounds treated with nanocrystalline silver was
characterized by reduced levels of matrix metalloproteinases. Matrix
metalloproteinases have been shown to be present in chronic ulcers at abnormally
high levels, as compared with acute wounds, and may contribute to the nonhealing
nature of these wounds. Cellular apoptosis occurred at a higher frequency in the
nanocrystalline silver-treated wounds than in wounds dressed with other products.
The results suggest that nanocrystalline silver may play a role in altering or
compressing the inflammatory events in wounds and facilitating the early phases of
wound healing. These benefits are associated with reduced local matrix
metalloproteinase levels and enhanced cellular apoptosis.
AU - Wright, J. B.
AU - Lam, K.
AU - Buret, A. G.
AU - Olson, M. E.
AU - Burrell, R. E.
DB - Scopus
DO - 10.1046/j.1524-475X.2002.10308.x
IS - 3
KW - Animals
Apoptosis
Bandages
Endopeptidases
Fusobacterium Infections
Granulation Tissue
Models, Animal
Polyesters
Polyethylenes
Pseudomonas Infections
Swine
Wound Healing
Wounds and Injuries
gelatinase A
gelatinase B
silver derivative
animal model
apoptosis
article
coagulase negative Staphylococcus
controlled study
full thickness skin graft
Fusobacterium
granulation tissue
histopathology
nonhuman
priority journal
protein degradation
protein expression
skin blood flow
swine
wound dressing
wound healing
wound infection
zymography
M3 - Article
PY - 2002
SP - 141-151
ST - Early healing events in a porcine model of contaminated wounds: Effects of
nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing
T2 - Wound Repair and Regeneration
TI - Early healing events in a porcine model of contaminated wounds: Effects of
nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing
0036310367&doi=10.1046%2fj.1524-
475X.2002.10308.x&partnerID=40&md5=0adff42a5751552dbacf4f2d35ada1a0
VL - 10
ID - 5760
ER -
TY - JOUR
AB - Silver selenide quantum dots (Ag2Se QDs) provide bright prospects for the
application of QDs in the field of biomedicine because they contain low-toxic
compounds and show great advantages in the imaging of deep tissues and tiny
vascular structures. However, the biosafety of these novel QDs has not been
thoroughly evaluated, especially in one main target for toxicity-the central
nervous system (CNS). Our previous studies have suggested severe inflammatory
responses to cadmium-containing QDs in the hippocampus, which gives us a hint
regarding the risk assessment of Ag2Se QDs. In this study, microglial activation
followed by enhanced levels of pro-inflammatory cytokines was observed in the
hippocampus of mice intravenously injected with Ag2Se QDs. When using the
microglial BV2 cells to investigate the underlying mechanisms, we found that the
NLRP3 inflammasome activation was involved in the IL-1 beta-mediated inflammation
induced by Ag2Se QDs. On the one hand, Ag2Se QD-activated NF-kappa B participated
in the NLRP3 inflammasome priming and assembly as well as the pro-IL-1 beta
upregulation. On the other hand, Ag2Se QD-induced ROS generation, particularly
mtROS, triggered the NLRP3 inflammasome activation and resulted in active caspase-1
to process pro-IL-1 beta into mature IL-1 beta release. These findings not only
indicated that it is important to evaluate the biosafety of novel QDs, even those
containing low-toxic compounds, but also provided an unbiased and mechanism-based
risk assessment of similar nanoparticles.
AN - WOS:000502779900044
AU - Wu, T. S.
AU - Liang, X.
AU - He, K. Y.
AU - Wei, T. T.
AU - Wang, Y.
AU - Zou, L. Y.
AU - Bai, C. C.
AU - Liu, N.
AU - Zhang, T.
AU - Xue, Y. Y.
AU - Tang, M.
DA - NOV 21
DO - 10.1039/c9nr06778g
IS - 43
PY - 2019
SN - 2040-3364
2040-3372
SP - 20820-20836
ST - The role of NLRP3 inflammasome activation in the neuroinflammatory responses
to Ag2Se quantum dots in microglia
T2 - NANOSCALE
TI - The role of NLRP3 inflammasome activation in the neuroinflammatory responses
VL - 11
ID - 6701
ER -
TY - JOUR
AB - Despite the increasing number of neurotoxicological studies on metal-
containing nanoparticles (NPs), the NP-induced neuroinflammation has not yet been
well understood. This review provides a comprehensive understanding of inflammatory
responses to two typical metal-containing NPs, namely silver NPs (AgNPs) and
titanium dioxide NPs (TiO2-NPs). Ag-NPs and TiO2-NPs could translocate into the CNS
through damaged blood-brain barrier, nerve afferent signaling and eye-to-brain
ways, and even cell uptake. NPs could stimulate the activation of glial cells to
release proinflammatory cytokines and generate reactive oxygen species and nitric
oxide production, resulting in the neuroinflammation. The potential mechanisms of
Ag-NPs and TiO2-NPs causing inflammation are complex, including several immune
response relevant signaling pathways. Some parameters governing their ability to
cause neuroinflammation are presented as well.
AN - WOS:000427392700007
AU - Wu, T. S.
AU - Tang, M.
DA - JAN
DO - 10.2217/nnm-2017-0270
IS - 2
PY - 2018
SN - 1743-5889
1748-6963
SP - 233-249
ST - The inflammatory response to silver and titanium dioxide nanoparticles in the
T2 - NANOMEDICINE
TI - The inflammatory response to silver and titanium dioxide nanoparticles in the
VL - 13
ID - 6401
ER -
TY - JOUR
AB - Silver selenide quantum dots (Ag2Se QDs) provide bright prospects for the
application of QDs in the field of biomedicine because they contain low-toxic
compounds and show great advantages in the imaging of deep tissues and tiny
vascular structures. However, the biosafety of these novel QDs has not been
thoroughly evaluated, especially in one main target for toxicity - the central
nervous system (CNS). Our previous studies have suggested severe inflammatory
responses to cadmium-containing QDs in the hippocampus, which gives us a hint
regarding the risk assessment of Ag2Se QDs. In this study, microglial activation
followed by enhanced levels of pro-inflammatory cytokines was observed in the
hippocampus of mice intravenously injected with Ag2Se QDs. When using the
microglial BV2 cells to investigate the underlying mechanisms, we found that the
NLRP3 inflammasome activation was involved in the IL-1β-mediated inflammation
induced by Ag2Se QDs. On the one hand, Ag2Se QD-activated NF-κB participated in the
NLRP3 inflammasome priming and assembly as well as the pro-IL-1β upregulation. On
the other hand, Ag2Se QD-induced ROS generation, particularly mtROS, triggered the
NLRP3 inflammasome activation and resulted in active caspase-1 to process pro-IL-1β
into mature IL-1β release. These findings not only indicated that it is important
to evaluate the biosafety of novel QDs, even those containing low-toxic compounds,
but also provided an unbiased and mechanism-based risk assessment of similar
nanoparticles. © The Royal Society of Chemistry.
AU - Wu, T.
AU - Liang, X.
AU - He, K.
AU - Wei, T.
AU - Wang, Y.
AU - Zou, L.
AU - Bai, C.
AU - Liu, N.
AU - Zhang, T.
AU - Xue, Y.
AU - Tang, M.
DB - Scopus
DO - 10.1039/c9nr06778g
IS - 43
KW - Animals
Cell Line
Cell Survival
Cytokines
Hippocampus
Inflammasomes
Interleukin-1beta
Male
Mice
Mice, Inbred ICR
Microglia
NF-kappa B
Quantum Dots
RNA Interference
RNA, Small Interfering
Selenium
Silver
Up-Regulation
Biohazards
Chemical activation
Mammals
Nanocrystals
Risk assessment
Selenium compounds
cryopyrin
cytokine
inflammasome
interleukin 1beta
quantum dot
selenium
silver
Central nervous systems
Mechanism-based
Microglial activations
Ros generations
Silver selenides
Vascular structures
animal
cell line
cell survival
chemistry
cytology
drug effect
genetics
hippocampus
male
metabolism
microglia
mouse
pathology
RNA interference
upregulation
Silver compounds
M3 - Article
PY - 2019
SP - 20820-20836
ST - The role of NLRP3 inflammasome activation in the neuroinflammatory responses
T2 - Nanoscale
TI - The role of NLRP3 inflammasome activation in the neuroinflammatory responses
85074675319&doi=10.1039%2fc9nr06778g&partnerID=40&md5=f47db78f5f5b51796dac28a9c49ff
22c
VL - 11
ID - 5442
ER -
TY - JOUR
AB - Graphene is a novel two-dimensional nanomaterial with a growing number of
practical applications across numerous fields. In this work, we explored potential
biomedical applications of graphene oxide (GO) by systematically studying
antibacterial capacity of GO in both macrophages and animal models. Three types of
bacteria, including Klebsiella pneumoniae (Kp), Escherichia coli (E. coli) and P.
aeruginosa (Pa) were used for in vitro study. Kp was also selected as a
representative multidrug resistant (MDR) bacterium for in vivo study. In in vitro
study, GO effectively eradicated Kp in agar dishes and thus protected alveolar
macrophages (AM) from Kp infection in the culture. In the in vivo evaluation, GO
were introduced intranasally into mouse lungs followed by testing organ tissue
damage including lung, liver, spleen, and kidneys, polymorphonuclear neutrophil
(PMN) penetration, bacterial dissemination, and mortality in Kp-infected mice. We
found that GO can prohibit the growth and spread of Kp both in vitro and in vivo,
resulting in significantly increased cell survival rate, less tissue injury,
subdued inflammatory response, and prolonged mice survival. These findings indicate
that GO could be a promising biomaterial for effectively controlling MDR pathogens.
AN - WOS:000408597900001
AU - Wu, X.
AU - Tan, S. R.
AU - Xing, Y. Q.
AU - Pu, Q. Q.
AU - Wu, M.
AU - Zhao, J. X. J.
DA - SEP 1
DO - 10.1016/j.colsurfb.2017.05.024
PY - 2017
SN - 0927-7765
1873-4367
SP - 1-9
ST - Graphene oxide as an efficient antimicrobial nanomaterial for eradicating
multi-drug resistant bacteria in vitro and in vivo
TI - Graphene oxide as an efficient antimicrobial nanomaterial for eradicating
multi-drug resistant bacteria in vitro and in vivo
VL - 157
ID - 6752
ER -
TY - CHAP
AB - It is well known that polymer materials have been extensively investigated
and developed for various biomedical applications due to their palpable advantages,
including availability, good biocompatibility and biodegradability, ordinary
synthesis and characterizations, viable structure modularization, and simple self-
assemble process. Hybrid composition can gain many positive features from both
mixing materials, which are able to meet the requirements in the applications.
Encapsulation of metal nanoparticles in polymer matrices can serve many purposes:
(1) improving stability of metal nanoparticles; (2) reducing toxicity; (3) easy to
be multi-functionalized; and (4) improving collective properties. Silver
nanoparticles (AgNPs) have attracted increasing attention in general and specially
in biomaterial applications. AgNPs have prominent antimicrobial, anticancer,
antiviral, antioxidant, anti-inflammatory and wound healing effects. The biosafety
of AgNPs is a critical issue. Therefore, hybrid nanomaterials based on AgNPs and
polymers are highly significant structures since they integrate synergistically the
advantageous physical-chemical and biological properties of both AgNPs and
polymeric components, providing excellent functionality to the final material. This
chapter critically outlines AgNPs/polymer nanocomposites for various biomedical
applications such as antibacterial, anticancer, tissue engineering, wound healing
and antiviral applications. © 2021, Springer Nature Switzerland AG.
AU - Wu, Y.
DB - Scopus
DO - 10.1007/978-3-030-44259-0_9
KW - AgNPs/polymer nanocomposites
Antibacterial
Anticancer
Antiviral
Biomedical application
Tissue engineering
Wound healing
Biocompatibility
Biodegradability
Biodegradable polymers
Hybrid materials
Metal nanoparticles
Modular construction
Nanocomposites
Tissue
AgNP/polymer nanocomposite
Antibacterials
Antivirals
Polymer materials
Polymer-nanocomposite
PY - 2021
SP - 213-246
ST - An Overview of Applications of Silver-Based Polymer Nano Composite as
Biomaterials
T2 - Engineering Materials
TI - An Overview of Applications of Silver-Based Polymer Nano Composite as
Biomaterials
85126707104&doi=10.1007%2f978-3-030-44259-
0_9&partnerID=40&md5=b49504c71a81555eb6924645894fe1cf
ID - 5210
ER -
TY - JOUR
AB - Exposure to inhaled anthropogenic nanomaterials (NM) with dimension <100 nm
has been implicated in numerous adverse respiratory outcomes. Although studies have
identified key NM physiochemical determinants of pneumonic nanotoxicity, the
complex interactive and cumulative effects of NM exposure, especially in
individuals with preexisting inflammatory respiratory diseases, remain unclear.
Herein, the susceptibility of primary human small airway epithelial cells (SAEC)
exposed to a panel of reference NM, namely, CuO, ZnO, mild steel welding fume
(MSWF), and nanofractions of copier center particles (Nano-CCP), is examined in
normal and tumor necrosis factor alpha (TNF-alpha)-induced inflamed SAEC. Compared
to normal SAEC, inflamed cells display an increased susceptibility to NM-induced
cytotoxicity by 15-70% due to a higher basal level of intracellular reactive oxygen
species (ROS). Among the NM screened, ZnO, CuO, and Nano-CCP are observed to
trigger an overcompensatory response in normal SAEC, resulting in an increased
tolerance against subsequent oxidative insults. However, the inflamed SAEC fails to
adapt to the NM exposure due to an impaired nuclear factor erythroid 2-related
factor 2 (Nrf2)-mediated cytoprotective response. The findings reveal that
susceptibility to pulmonary nanotoxicity is highly dependent on the interplay
between NM properties and inflammation of the alveolar milieu.
AN - WOS:000528629600001
AU - Wu, Z. R.
AU - Shi, P. J.
AU - Lim, H. K.
AU - Ma, Y. Y.
AU - Setyawati, M. I.
AU - Bitounis, D.
AU - Demokritou, P.
AU - Ng, K. W.
AU - Tay, C. Y.
C6 - APR 2020
C7 - 2000963
DA - MAY
DO - 10.1002/smll.202000963
IS - 21
PY - 2020
SN - 1613-6810
1613-6829
ST - Inflammation Increases Susceptibility of Human Small Airway Epithelial Cells
to Pneumonic Nanotoxicity
T2 - SMALL
TI - Inflammation Increases Susceptibility of Human Small Airway Epithelial Cells
to Pneumonic Nanotoxicity
VL - 16
ID - 6572
ER -
TY - JOUR
AB - Biofilms play a key role in the development of chronic tissue infections and
antimicrobial resistance. In situ-forming thermosensitive hydrogel containing
biosynthesized silver nanoparticles using aqueous Eucalyptus camaldulensis leaf
extract (bio-AgNPs) was developed as an efficient anti-biofilm agent with a
sustained release for tissue infection treatment. The formulation with 24%
poloxamer 407, 5% poloxamer 188, and 1% hydroxypropyl methylcellulose demonstrated
gelation temperature at 33 °C and homogeneous dispersion of bio-AgNPs throughout
three-dimensional networks. Pharmaceutical properties of bio-AgNPs-loaded hydrogel
presented shear-thinning behavior and zero-order release profile. The formulation
showed broad-spectrum antimicrobial activity and comparable effects with existing
antibiotics against important pathogens, including Gram-positive, Gram-negative
bacteria, and fungi. The formulation at 1/8–1/2 minimum inhibitory concentrations
(MIC) significantly inhibited biofilm production in all tested pathogens (p <
0.05). Confocal laser scanning microscopy images clearly illustrated dead cells
within mature biofilm upon the treatment with the formulation at 2MIC for 4 h. The
formulation effectively scavenged free radicals and decreased nitric oxide
production up to 98%. Decrease in mRNA levels of inflammation-related enzymes and
pro-inflammatory cytokines in lipopolysaccharide-stimulated RAW 264.7 cells
confirmed the formulation as a potential anti-inflammatory agent. The findings
suggested that bio-AgNPs-loaded hydrogel represents a promising therapeutic
approach for tissue infections. © 2022
AU - Wunnoo, S.
AU - Bilhman, S.
AU - Waen‐ngoen, T.
AU - Yawaraya, S.
AU - Paosen, S.
AU - Lethongkam, S.
AU - Kaewnopparat, N.
AU - Voravuthikunchai, S. P.
C7 - 103588
DB - Scopus
DO - 10.1016/j.jddst.2022.103588
Antibiofilm
Antimicrobial
Eucalyptus camaldulensis
Nanocomposite hydrogel
Eucalyptus camaldulensis extract
free radical
hydrogel
lipopolysaccharide
messenger RNA
nitric oxide
plant extract
poloxamer
silver nanoparticle
unclassified drug
animal cell
Article
biosynthesis
controlled study
cross linking
cytotoxicity
dispersion
drug formulation
flow kinetics
freeze thawing
fungus
gelation
gene expression
heat sensitivity
in vitro study
infection resistance
inflammation
nonhuman
plant leaf
pore size
pulp and paper industry
RAW 264.7 cell line
reaction temperature
surface property
M3 - Article
PY - 2022
ST - Thermosensitive hydrogel loaded with biosynthesized silver nanoparticles
using Eucalyptus camaldulensis leaf extract as an alternative treatment for
microbial biofilms and persistent cells in tissue infections
T2 - Journal of Drug Delivery Science and Technology
TI - Thermosensitive hydrogel loaded with biosynthesized silver nanoparticles
using Eucalyptus camaldulensis leaf extract as an alternative treatment for
microbial biofilms and persistent cells in tissue infections
85134784171&doi=10.1016%2fj.jddst.2022.103588&partnerID=40&md5=f1d8623006d5e8ec6e4a
99e63a8d56a8
VL - 74
ID - 5076
ER -
TY - JOUR
AB - The development of nanotechnology in the last two decades has led to the use
of silver nanoparticles (AgNPs) in various biomedical applications, including
antimicrobial, anti-inflammatory, and anticancer therapies. However, the potential
of the medical application of AgNPs depends on the safety of their use. In this
work, we assessed the in vitro cytotoxicity and genotoxicity of silver
nanoparticles and identified biomolecules covering AgNPs synthesized from
actinobacterial strain SH11. The cytotoxicity of AgNPs against MCF-7 human breast
cancer cell line and murine macrophage cell line RAW 264.7 was studied by MTT
assay, cell LDH (lactate dehydrogenase) release, and the measurement of ROS
(reactive oxygen species) level while genotoxicity in Salmonella typhimurium cells
was testing using the Ames test. The in vitro analysis showed that the tested
nanoparticles demonstrated dose-dependent cytotoxicity against RAW264.6 macrophages
and MCF-7 breast cancer cells. Moreover, biosynthesizedAgNPsdid not show a
mutagenic effect of S. typhimurium. The analyses and identification of biomolecules
present on the surface of silver nanoparticles showed that they were associated
with proteins. The SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel
electrophoresis) analysis revealed the presence of 34 and 43 kDa protein bands. The
identification of proteins performed by using LC-MS/MS (liquid chromatography with
tandem mass spectrometry) demonstrated their highest homology to bacterial porins.
Capping biomolecules of natural origin may be involved in the synthesis process of
AgNPs or may be responsible for their stabilization. Moreover, the presence of
natural proteins on the surface of bionanoparticles eliminates the postproduction
steps of capping which is necessary for chemical synthesis to obtain the stable
nanostructures required for application in medicine. © 2020 MDPI AG. All rights
reserved.
AU - Wypij, M.
AU - Jedrzejewski, T.
AU - Ostrowski, M.
AU - Trzcinska, J.
AU - Rai, M.
AU - Golinska, P.
C7 - 3022
DB - Scopus
DO - 10.3390/molecules25133022
IS - 13
KW - Biosynthesis
Capping proteins
Cytotoxicity
Genotoxicity
Actinobacteria
Animals
Bacterial Proteins
Cytotoxins
Electrophoresis, Polyacrylamide Gel
Humans
MCF-7 Cells
Metal Nanoparticles
Mice
Mutagenicity Tests
RAW 264.7 Cells
Silver
bacterial protein
cytotoxin
metal nanoparticle
silver
animal
chemistry
dose response
drug effect
genetics
human
MCF-7 cell line
metabolism
mouse
mutagen testing
procedures
RAW 264.7 cell line
M3 - Article
PY - 2020
ST - Biogenic silver nanoparticles: Assessment of their cytotoxicity, genotoxicity
and study of capping proteins
T2 - Molecules
TI - Biogenic silver nanoparticles: Assessment of their cytotoxicity, genotoxicity
and study of capping proteins
85087730852&doi=10.3390%2fmolecules25133022&partnerID=40&md5=7e7b784b4d2c53132b746e
87328a376b
VL - 25
ID - 5285
ER -
TY - JOUR
AB - Nanoparticles (NPs) interact with biomolecules by forming a biocorona (BC) on
their surface after introduction into the body and alter cell interactions and
toxicity. Metabolic syndrome (MetS) is a prevalent condition and enhances
susceptibility to inhaled exposures. We hypothesize that distinct NP-biomolecule
interactions occur in the lungs due to MetS resulting in the formation of unique
NP-BCs contributing to enhanced toxicity. Bronchoalveolar lavage fluid (BALF) was
collected from healthy and MetS mouse models and used to evaluate variations in the
BC formation on 20 nm iron oxide (Fe3O4) NPs. Fe3O4 NPs without or with BCs were
characterized for hydrodynamic size and zeta potential. Unique and differentially
associated proteins and lipids with the Fe3O4 NPs were identified through proteomic
and lipidomic analyses to evaluate BC alterations based on disease state. A mouse
macrophage cell line was utilized to examine alterations in cell interactions and
toxicity due to BCs. Exposures to 6.25, 12.5, 25, and 50 mu g/mL of Fe3O4 NPs with
BCs for 1 h or 24 h did not demonstrate overt cytotoxicity. Macrophages
increasingly associated Fe3O4 NPs following addition of the MetS BC compared to the
healthy BC. Macrophages exposed to Fe3O4 NPs with a MetS-BC for 1 h or 24 h at a
concentration of 25 mu g/mL demonstrated enhanced gene expression of inflammatory
markers: CCL2, IL-6, and TNF-alpha compared to Fe3O4 NPs with a healthy BC. Western
blot analysis revealed activation of STAT3, NF-kappa B, and ERK pathways due to the
MetS-BC. Specifically, the Jak/Stat pathway was the most upregulated inflammatory
pathway following exposure to NPs with a MetS BC. Overall, our study suggests the
formation of distinct BCs due to NP exposure in MetS, which may contribute to
exacerbated inflammatory effects and susceptibility.
AN - WOS:000818149500001
AU - Xia, L.
AU - Alqahtani, S.
AU - Ferreira, C. R.
AU - Aryal, U. K.
AU - Biggs, K.
C7 - 2022
DA - JUN
DO - 10.3390/nano12122022
IS - 12
PY - 2022
SN - 2079-4991
ST - Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in
Iron Oxide Nanoparticle-Biocorona Composition
T2 - NANOMATERIALS
TI - Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in
Iron Oxide Nanoparticle-Biocorona Composition
VL - 12
ID - 6422
ER -
TY - JOUR
AB - Bacterial infection has become one of the most challenges for wound healing,
which causes serious inflammatory response and delays the healing process. Herein,
a novel sponge with excellent biocompatible, antibacterial and anti-inflammatory
properties based on quaternized cellulose (QC), sodium alginate (SA) and Zn2+ was
reported. The existence of physical interactions, such as electrostatic
interaction, chelation and hydrogen bonding endowed the sponges with enhanced
mechanical property. The composite sponges exhibited outstanding biocompatibility
and hemostatic efficiency due to the compatible nature of the component and
physical crosslinking, as well as superior antibacterial property benefited from
the synergistic effects of steady Zn2+ release and quaternary ammonium group. In
vivo investigation validated that the enhanced antibacterial and antiinflammatory
effect of the sponges, which significantly promoted wound closure and the
reconstruction of skin tissue through epithelial regeneration, collagen deposition
and mitigating inflammatory cell infiltration. Overall, the novel sponge
demonstrated great potentials in bacteria-associated wound management.
AN - WOS:000710832500004
AU - Xie, H. X.
AU - Xia, H. Y.
AU - Huang, L.
AU - Zhong, Z. B. A.
AU - Ye, Q. F.
AU - Zhang, L. N.
AU - Lu, A.
C6 - SEP 2021
DA - NOV 30
DO - 10.1016/j.ijbiomac.2021.09.047
PY - 2021
SN - 0141-8130
1879-0003
SP - 27-39
ST - Biocompatible, antibacterial and anti-inflammatory zinc ion cross-linked
quaternized cellulose-sodium alginate composite sponges for accelerated wound
healing
TI - Biocompatible, antibacterial and anti-inflammatory zinc ion cross-linked
quaternized cellulose-sodium alginate composite sponges for accelerated wound
healing
VL - 191
ID - 6026
ER -
TY - JOUR
AB - Diabetic nephropathy (DN) is the leading cause of end-stage renal disease
worldwide. Autophagy was reported to be related to the pathogenesis of DN. This
research investigated the function of the Nucleoporin 160 (Nup160) gene in
regulating autophagy in DN. A mouse model of DN was established through an
intraperitoneal injection of streptozotocin (STZ). Normal rat kidney tubular
epithelial cells (NRK-52E) were treated with high glucose to induce DN in vitro.
Real-time quantitative polymerase chain reaction (RT-qPCR), western blot,
immunofluorescence assays were conducted to measure the expression of NUP160,
autophagy-associated proteins, and inflammatory cytokines in vitro and in vivo.
Pathological changes of kidney and liver tissues were analyzed using hematoxylin
and eosin (H&E), Masson and periodic acid-silver (PAS) staining. The body weight,
blood glucose, renal and lipid profiles of DN mice were examined. In this study, DN
mice showed serious pathological injury. NUP160 expression was upregulated,
autophagy was inhibited, and inflammatory response was increased in DN mice.
Depletion of NUP160 restored autophagy and inhibited inflammation and fibrosis in
high glucose (HG)-treated NRK-52E cells and STZ-induced DN mice by downregulating
the expression of p62 and Collagen IV (Col-), increasing the ratio of LC3II/LC3I,
and inactivating nuclear factor (NF)-kappa B signaling. Moreover, NUP160 knockdown
could ameliorate pathological damage and glucose tolerance in DN mice. Overall,
this study is the first to demonstrate the key role of NUP160 silencing in
promoting autophagy against diabetic injury in DN.
AN - WOS:000696812100001
AU - Xie, J. Y.
AU - Yuan, Y.
AU - Yao, G.
AU - Chen, Z.
AU - Yu, W. J.
AU - Zhu, Q.
DA - JAN 1
DO - 10.1080/21655979.2021.1968777
IS - 1
PY - 2021
SN - 2165-5979
2165-5987
SP - 6390-6402
ST - Nucleoporin 160 (NUP160) inhibition alleviates diabetic nephropathy by
activating autophagy
T2 - BIOENGINEERED
TI - Nucleoporin 160 (NUP160) inhibition alleviates diabetic nephropathy by
VL - 12
ID - 6543
ER -
TY - JOUR
AB - Citrate-modified silver nanoparticles (AgNP-cit) have received extensive
attention due to their excellent antimicrobial properties. However, these particles
tend to migrate in vivo, thereby entering the blood circulatory system in granular
form and accumulating in the liver, causing toxic reactions. However, the mechanism
underlying AgNP-cit toxicity is not yet clear. Thus, we adopted a tandem mass tag
(TMT)-labeled quantitative proteomics and metabolomics approach to identify
proteins and small molecule metabolites associated with AgNP-cit-induced liver
damage and constructed interaction networks between the differentially expressed
proteins and metabolites to explain the AgNP-cit toxicity mechanism. AgNP-cit
resulted in abnormal purine metabolism mainly by affecting xanthine and other key
metabolites along with pyruvate kinase and other bodily proteins, leading to
oxidative stress. AgNP-cit regulated the metabolism of amino acids and glycerol
phospholipids through glycerol phospholipids, CYP450 enzymes and other key
proteins, causing liver inflammation. Via alanine, isoleucine, L-serine
dehydratase/L-threonine deaminase and other proteins, AgNP-cit altered the
metabolism of glycine, serine and threonine, cysteine and methionine, affecting
oxidation and deamination, and ultimately leading to liver damage. This work
clearly explains toxic reactions induced by AgNP-cit from three perspectives,
oxidative stress, inflammatory response, and oxidation and deamination, thus
providing an experimental basis for the safe application of nanomaterials. © 2017
AU - Xie, J.
AU - Dong, W.
AU - Liu, R.
AU - Wang, Y.
AU - Li, Y.
DB - Scopus
DO - 10.1080/17435390.2017.1415389
IS - 1
KW - Citrate-modified silver nanoparticles
systems biology analysis
UPLC/Q-TOF-MS
Animals
Citric Acid
Male
Metabolome
Metabolomics
Metal Nanoparticles
Oxidative Stress
Proteome
Proteomics
Rats
Silver
alanine
amino acid
biological marker
citric acid
cysteine
cytochrome P450
glycerol
glycine
isoleucine
methionine
phospholipid
pyruvate kinase
serine
serine dehydratase
silver nanoparticle
threonine
threonine ammonia lyase
xanthine
metal nanoparticle
proteome
silver
amino acid metabolism
animal experiment
animal model
animal tissue
Article
controlled study
deamination
histopathology
inflammation
liver injury
liver toxicity
male
metabolite
metabolomics
nonhuman
oxidation
oxidative stress
pathogenesis
priority journal
protein expression
protein metabolism
proteomics
rat
tandem mass tag
time of flight mass spectrometry
animal
chemistry
drug effect
metabolism
metabolome
toxic hepatitis
M3 - Article
PY - 2018
SP - 18-31
ST - Research on the hepatotoxicity mechanism of citrate-modified silver
nanoparticles based on metabolomics and proteomics
T2 - Nanotoxicology
TI - Research on the hepatotoxicity mechanism of citrate-modified silver
nanoparticles based on metabolomics and proteomics
85038371059&doi=10.1080%2f17435390.2017.1415389&partnerID=40&md5=6107e2b8f9582abaaf
601204a4e434ba
VL - 12
ID - 5520
ER -
TY - JOUR
AB - Diabetic nephropathy (DN) is the leading cause of end-stage renal disease
worldwide. Autophagy was reported to be related to the pathogenesis of DN. This
research investigated the function of the Nucleoporin 160 (Nup160) gene in
regulating autophagy in DN. A mouse model of DN was established through an
intraperitoneal injection of streptozotocin (STZ). Normal rat kidney tubular
epithelial cells (NRK-52E) were treated with high glucose to induce DN in vitro.
Real-time quantitative polymerase chain reaction (RT-qPCR), western blot,
immunofluorescence assays were conducted to measure the expression of NUP160,
autophagy-associated proteins, and inflammatory cytokines in vitro and in vivo.
Pathological changes of kidney and liver tissues were analyzed using hematoxylin
and eosin (H&E), Masson and periodic acid-silver (PAS) staining. The body weight,
blood glucose, renal and lipid profiles of DN mice were examined. In this study, DN
mice showed serious pathological injury. NUP160 expression was upregulated,
autophagy was inhibited, and inflammatory response was increased in DN mice.
Depletion of NUP160 restored autophagy and inhibited inflammation and fibrosis in
high glucose (HG)-treated NRK-52E cells and STZ-induced DN mice by downregulating
the expression of p62 and Collagen IV (Col-Ⅳ), increasing the ratio of LC3II/LC3I,
and inactivating nuclear factor (NF)-κB signaling. Moreover, NUP160 knockdown could
ameliorate pathological damage and glucose tolerance in DN mice. Overall, this
study is the first to demonstrate the key role of NUP160 silencing in promoting
autophagy against diabetic injury in DN. © 2021 The Author(s). Published by Informa
UK Limited, trading as Taylor & Francis Group.
AU - Xie, J.
AU - Yuan, Y.
AU - Yao, G.
AU - Chen, Z.
AU - Yu, W.
AU - Zhu, Q.
DB - Scopus
DO - 10.1080/21655979.2021.1968777
IS - 1
KW - autophagy
NUP160
Animals
Autophagy
Cells, Cultured
Diabetic Nephropathies
Fibrosis
Inflammation
Male
Mice
Mice, Inbred C57BL
Nuclear Pore Complex Proteins
Rats
Signal Transduction
adiponectin
albumin
buffer
collagen type 4
creatinine
cryopyrin
cytokine
eosin
fibronectin
glucose
hematoxylin
inflammasome
interleukin 1beta
interleukin 6
irbesartan
lipofectamine
nephrin
nucleoporin
pentobarbital
periodic acid
sequestosome 1
streptozocin
synaptotagmin I
triacylglycerol
animal cell
animal experiment
animal model
animal tissue
Article
autophagosome
blood brain barrier
blood glucose monitoring
blood sampling
chemoluminescence
controlled study
down regulation
drug toxicity
end stage renal disease
epithelium cell
glucose blood level
glucose intake
glucose tolerance
glycolysis
histology
immunofluorescence
insulin blood level
kidney biopsy
kidney fibrosis
lipid fingerprinting
lipid storage
male
NF kB signaling
nonhuman
oral glucose tolerance test
protein expression
protein function
rat
real time reverse transcription polymerase chain reaction
signal transduction
silver staining
streptozotocin-induced diabetic nephropathy
upregulation
Western blotting
animal
C57BL mouse
cell culture
fibrosis
genetics
inflammation
metabolism
mouse
M3 - Article
PY - 2021
SP - 6390-6402
ST - Nucleoporin 160 (NUP160) inhibition alleviates diabetic nephropathy by
T2 - Bioengineered
TI - Nucleoporin 160 (NUP160) inhibition alleviates diabetic nephropathy by
85115206193&doi=10.1080%2f21655979.2021.1968777&partnerID=40&md5=4f0c94d4286bc8f5ce
c33887aa861b82
VL - 12
ID - 5192
ER -
TY - JOUR
AB - Background: Drug resistance of pathogens and immunosuppression are the main
causes of clinical stagnation of osteomyelitis. The ideal treatment strategy for
osteomyelitis is to achieve both efficient antibacterial and bone healing through
spatiotemporal modulation of immune microenvironment. Methods: In this study, a
bilayer hydrogel based on genetically engineered polypeptide AC10A and AC10ARGD was
prepared by self-assembly. Ag2S QDs@DSPE-mPEG2000-Ce6/Aptamer (AD-Ce6/Apt) was
loaded in the top layer AC10A hydrogel (AA) for antibacterial, and bone marrow-
derived mesenchymal stem cells (BMSCs) were loaded in the lower layer AC10ARGD
hydrogel (MAR) for bone healing. The AD-Ce6/Apt can be released from the AA
hydrogel to target S. aureus before bacterial biofilm formation and achieved
significant bactericidal effect under irradiation with a 660 nm laser. Moreover,
AD-Ce6/Apt can induce M1 type polarization of macrophages to activate the immune
system and eliminate residual bacteria. Subsequently, BMSCs released from the MAR
hydrogel can differentiate into osteoblasts and promote the formation of an anti-
inflammatory microenvironment by regulating the M2 type polarization of
macrophages. The bilayer AA-MAR hydrogel possessed good biocompatibility. Results:
The in vitro and in vivo results showed that the AA-MAR hydrogel not only realized
efficient photodynamic therapy of S. aureus infection, but also promoted the
transformation of immune microenvironment to fulfill the different needs of each
stage, which ultimately improved bone regeneration and mechanical properties post-
surgery. Conclusion: This work presents an approach for spatiotemporal modulation
of immune microenvironment in the treatment of osteomyelitis. Graphical Abstract:
[Figure not available: see fulltext.]. © 2022, The Author(s).
AU - Xie, X.
AU - Wei, J.
AU - Zhang, B.
AU - Xiong, W.
AU - He, Z.
AU - Zhang, Y.
AU - Gao, C.
AU - Zhao, Y.
AU - Liu, B.
C7 - 416
DB - Scopus
DO - 10.1186/s12951-022-01614-3
IS - 1
KW - Antibacterial
Bone repairing
Engineered polypeptide hydrogel
Osteomyelitis
Spatiotemporal regulation
Dimaprit
Humans
Hydrogels
Peptides
Biocompatibility
Macrophages
Modulation
Polarization
Silver compounds
Stem cells
Tissue regeneration
1,2 distearoyl sn glycero 3 phosphoethanolamine
aptamer
chlorin e6
glycerophospholipid
hydrogel
interleukin 6
macrogol 2000
photosensitizing agent
polypeptide
polypeptide AC10A
polypeptide AC10ARGD
quantum dot
silver derivative
silver sulfide
unclassified drug
antiinfective agent
APT
dimaprit
peptide
Antibacterials
Aptamers
Bi-layer
Bone healing
Microenvironments
Polypeptide hydrogels
animal cell
animal experiment
animal model
animal tissue
Article
bacterial clearance
biocompatibility
bioengineering
biofilm
bone regeneration
bone remodeling
controlled study
drug degradation
drug release
fracture healing
immune system
immunomodulation
in vitro study
in vivo study
laser therapy
M1 macrophage
M2 macrophage
microenvironment
nonhuman
osteoblast
osteomyelitis
photodynamic therapy
polarization
rat
spatiotemporal analysis
chemistry
human
Cell culture
M3 - Article
PY - 2022
ST - A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal
modulation of bactericidal and anti-inflammation process in osteomyelitis treatment
TI - A self-assembled bilayer polypeptide-engineered hydrogel for spatiotemporal
modulation of bactericidal and anti-inflammation process in osteomyelitis treatment
85137910944&doi=10.1186%2fs12951-022-01614-
3&partnerID=40&md5=abb89cd8273dc828bb11e2263344e94e
VL - 20
ID - 4984
ER -
TY - JOUR
AB - Graphene, including graphene quantum dots, its oxide and unoxidized forms
(pure graphene) have several properties, like fluorescence, electrical
conductivity, theoretical surface area, low toxicity, and high biocompatibility. In
this study, we evaluated genotoxicity (in silico analysis using the functional
density theory-FDT), cytotoxicity (human glioblastoma cell line), in vivo
pharmacokinetics, in vivo impact on microcirculation and cell internalization
assay. It was also radiolabeled with lutetium 177 (177Lu), a beta emitter
radioisotope to explore its therapeutic use as nanodrug. Finally, the impact of its
disposal in the environment was analyzed using ecotoxicological evaluation. FDT
analysis demonstrated that graphene can construct covalent and non-covalent bonds
with different nucleobases, and graphene oxide is responsible for generation of
reactive oxygen species (ROS), corroborating its genotoxicity. On the other hand,
non-cytotoxic effect on glioblastoma cells could be demonstrated. The
pharmacokinetics analysis showed high plasmatic concentration and clearance.
Topical application of 0.1 and 1 mg/kg of graphene nanoparticles on the hamster
skinfold preparation did not show inflammatory effect. The cell internalization
assay showed that 1-hour post contact with cells, graphene can cross the plasmatic
membrane and accumulate in the cytoplasm. Radio labeling with 177Lu is possible and
its use as therapeutic nanosystem is viable. Finally, the ecotoxicity analysis
showed that A. silina exposed to graphene showed pronounced uptake and absorption
in the nauplii gut and formation of ROS. The data obtained showed that although
being formed exclusively of carbon and carbon-oxygen, graphene and graphene oxide
respectively generate somewhat contradictory results and more studies should be
performed to certify the safety use of this nanoplatform.
AN - WOS:000625640100001
AU - Xing, H. X.
AU - de Barros, A. O. D.
AU - Mello, Fdce
AU - Sozzi-Guo, F.
AU - Muller, C.
AU - Gemini-Piperni, S.
AU - Alencar, L. M. R.
AU - Maia, F. F.
AU - Freire, V. N.
AU - de Menezes, F. D.
AU - Aran, V.
AU - Devalle, S.
AU - Moura-Neto, V.
AU - Ricci, E.
AU - Bouskela, E.
AU - Pikula, K.
AU - Golokhvast, K.
AU - Santos-Oliveira, R.
DA - JAN
DO - 10.1166/jbn.2021.3006
IS - 1
PY - 2021
SN - 1550-7033
1550-7041
SP - 131-148
ST - Graphene: Insights on Biological, Radiochemical and Ecotoxicological Aspects
TI - Graphene: Insights on Biological, Radiochemical and Ecotoxicological Aspects
VL - 17
ID - 6464
ER -
TY - JOUR
AB - Acrolein is a reactive unsaturated aldehyde and is found at high
concentrations in both mainstream and side-stream tobacco smoke. Exposure to
acrolein via cigarette smoking has been associated with acute lung injury, chronic
obstructive pulmonary diseases (COPDs), and asthma. In this study, we developed an
in vitro treatment strategy that resembles the inhalation exposure to acrolein
experienced by smokers and systematically examined the adverse respiratory effects
induced by the noncytotoxic doses of acrolein in a human airway epithelial tissue
model. A single 10-min exposure to buffered saline containing acrolein
significantly induced oxidative stress and inflammatory responses, with changes in
protein oxidation and GSH depletion occurring immediately after the treatment
whereas responses in inflammation requiring a manifestation time of at least 24 h.
Repeated exposure to acrolein for 10 consecutive days resulted in structural and
functional changes that recapitulate the pathological lesions of COPD, including
alterations in the beating frequency and structures of ciliated cells, inhibition
of mucin expression and secretion apparatus, and development of squamous
differentiation. Although some of the early responses caused by acrolein exposure
were reversible after a 10-day recovery, perturbations in the functions and
structures of the air-liquid-interface (ALI) cultures, such as mucin production,
cilia structures, and morphological changes, failed to fully recover over the
observation period. Taken together, these findings are consistent with its mode of
action that oxidative stress and inflammation have fundamental roles in acrolein-
induced tissue remodeling. Furthermore, these data demonstrate the usefulness of
analytical methods and testing strategy for recapitulating the key events in
acrolein toxicity using an in vitro model.
AN - WOS:000456554100019
AU - Xiong, R.
AU - Wu, Q. G.
AU - Muskhelishvili, L.
AU - Davis, K.
AU - Shemansky, J. M.
AU - Bryant, M.
AU - Rosenfeldt, H.
AU - Healy, S. M.
AU - Cao, X. F.
DA - DEC
DO - 10.1093/toxsci/kfy226
IS - 2
PY - 2018
SN - 1096-6080
1096-0929
SP - 451-464
ST - Evaluating Mode of Action of Acrolein Toxicity in an In Vitro Human Airway
Tissue Model
TI - Evaluating Mode of Action of Acrolein Toxicity in an In Vitro Human Airway
Tissue Model
VL - 166
ID - 6283
ER -
TY - JOUR
AB - Cadmium (Cd) is found at high concentrations in tobacco smoke due to its
volatility when tobacco is burned. Inhaled Cd is linked to smoking-related
respiratory diseases, such as chronic obstructive pulmonary disease and lung
cancer. Alterations in mucociliary clearance, squamous metaplasia, and carcinoma
are commonly observed in the respiratory tract of animals exposed to Cd. In vitro
cell models widely used to study mechanisms underlying Cd toxicity are not suitable
for studying its effects on mucociliary clearance and airway tissue remodeling.
Herein we assess Cd-induced functional and structural changes in a well-
differentiated human air-liquid-interface (ALI) airway tissue model. Acute
treatments with Cd induced aberrant expression and secretion of mucins, impaired
cilia functions, and squamous differentiation, and produced persistent oxidative
stress and enhanced release of pro-inflammatory cytokines and matrix
metalloproteinases. Accumulation of intracellular Cd was associated with sustained
oxidative stress and inflammation, which, in turn, may have initiated squamous
differentiation in ALI cultures. These observations demonstrate that ALI airway
tissue models can recapitulate the functional and structural alterations in Cd-
exposed animals, suggesting their potential application for studying tissue
responses related to respiratory toxicants like those present in tobacco smoke.
AN - WOS:000456000400003
AU - Xiong, R.
AU - Wu, Q. G.
AU - Trbojevich, R.
AU - Davis, K.
AU - Bryant, M.
AU - Richter, P.
AU - Cao, X. F.
DA - MAR 15
DO - 10.1016/j.toxlet.2018.12.009
PY - 2019
SN - 0378-4274
1879-3169
SP - 16-27
ST - Disease-related responses induced by cadmium in an in vitro human airway
tissue model
TI - Disease-related responses induced by cadmium in an in vitro human airway
tissue model
VL - 303
ID - 6459
ER -
TY - JOUR
AB - Exposure to cigarette smoke (CS) is a known risk factor in the pathogenesis
of smoking-caused diseases, such as chronic obstructive pulmonary diseases (COPD)
and lung cancer. To assess the effects of CS on the function and phenotype of
airway epithelial cells, we developed a novel repeated treatment protocol and
comprehensively evaluated the progression of key molecular, functional, and
structural abnormalities induced by CS in a human in vitro air-liquid-interface
(ALI) airway tissue model. Cultures were exposed to CS (diluted with 0.5 L/min, 1.0
L/min, and 4.0 L/min clean air) generated from smoking five 3R4F University of
Kentucky reference cigarettes under the International Organization for
Standardization (ISO) machine smoking regimen, every other day for 4 weeks (3 days
per week, 40 min/day). By integrating the transcriptomics-based approach with the
in vitro pathophysiological measurements, we demonstrated CS-mediated effects on
oxidative stress, pro-inflammatory cytokines and matrix metalloproteinases (MMPs),
ciliary function, expression and secretion of mucins, and squamous cell
differentiation that are highly consistent with abnormalities observed in airways
of smokers. Enrichment analysis on the transcriptomic profiles of the ALI cultures
revealed key molecular pathways, such as xenobiotic metabolism, oxidative stress,
and inflammatory responses that were perturbed in response to CS exposure. These
responses, in turn, may trigger aberrant tissue remodeling, eventually leading to
the onset of respiratory diseases. Furthermore, changes of a panel of genes known
to be disturbed in smokers with COPD were successfully reproduced in the ALI
cultures exposed to CS. In summary, findings from this study suggest that such an
integrative approach may be a useful tool for identifying genes and adverse
cellular events caused by inhaled toxicants, like CS.
AN - WOS:000625041000001
AU - Xiong, R.
AU - Wu, Y.
AU - Wu, Q. G.
AU - Davis, K.
AU - Tripathi, P.
AU - Chen, Y.
AU - Chen, T.
AU - Bryant, M.
AU - Rosenfeldt, H.
AU - Healy, S. M.
AU - Cao, X. F.
C6 - MAR 2021
DA - MAY
DO - 10.1007/s00204-021-03008-0
IS - 5
PY - 2021
SN - 0340-5761
1432-0738
SP - 1739-1761
ST - Integration of transcriptome analysis with pathophysiological endpoints to
evaluate cigarette smoke toxicity in an in vitro human airway tissue model
TI - Integration of transcriptome analysis with pathophysiological endpoints to
evaluate cigarette smoke toxicity in an in vitro human airway tissue model
VL - 95
ID - 6438
ER -
TY - JOUR
AB - Burn infection delays wound healing and increases the burn patient mortality.
Consequently, a new dressing with antibacterial and anti-inflammatory dual
properties is urgently required for wound healing. In this study, we propose a
combination of methacrylate gelatin (GelMA) hydrogel system with silver
nanoparticles embed in γ-cyclodextrin metal–organic frameworks (Ag@MOF) and
hyaluronic acid-epigallocatechin gallate (HA-E) for the burn wound infection
treatment. Ag@MOF is used as an antibacterial agent and epigallocatechin gallate
(EGCG) has exhibited biological properties of anti-inflammation and antibacterial.
The GelMA/HA-E/Ag@MOF hydrogel enjoys suitable physical properties and sustained
release of Ag+. Meanwhile, the hydrogel has excellent biocompatibility and could
promote macrophage polarization from M1 to M2. In vivo wound healing evaluations
further demonstrate that the GelMA/HA-E/Ag@MOF hydrogel reduces the number of the
bacterium efficiently, accelerates wound healing, promotes early angiogenesis, and
regulates immune reaction. A further evaluation indicates that the noncanonical Wnt
signal pathway is significantly activated in the GelMA/HA-E/Ag@MOF hydrogel treated
group. In conclusion, the GelMA/HA-E/Ag@MOF hydrogel could serve as a promising
multifunctional dressing for the burn wound healing. © 2022 The Authors.
Bioengineering & Translational Medicine published by Wiley Periodicals LLC on
behalf of American Institute of Chemical Engineers.
AU - Xiong, Y.
AU - Xu, Y.
AU - Zhou, F.
AU - Hu, Y.
AU - Zhao, J.
AU - Liu, Z.
AU - Zhai, Q.
AU - Qi, S.
AU - Zhang, Z.
AU - Chen, L.
C7 - e10373
DB - Scopus
DO - 10.1002/btm2.10373
IS - 1
KW - Ag-metal–organic framework
burn wound infection
macrophage polarization
methacrylate gelatin
Biocompatibility
Hyaluronic acid
Hydrogels
Macrophages
Metal nanoparticles
alkali metal
antiinfective agent
collagen
cyclodextrin
gamma cyclodextrin
gelatin
hyaluronic acid
hyaluronidase
hydrogel
pentobarbital
silver nanoparticle
Anti-inflammatories
Antibacterials
Burn wound infection
Macrophage polarization
Methacrylate gelatin
Wound healing
Wound infections
3T3 cell line
angiogenesis
animal cell
animal experiment
animal model
animal tissue
Article
bacterial growth
biocompatibility
blood flow
burn infection
cell adhesion assay
cell migration
cell proliferation
chemoluminescence
coculture
colony forming unit
controlled study
dispersity
epithelium cell
Escherichia coli
foreign body giant cell
freeze drying
hemostasis
histology
inflammation
macrophage
nonhuman
polarization
pore size
rat
RAW 264.7 cell line
Western blotting
Wnt signaling
wound healing
wound infection
Polarization
M3 - Article
PY - 2023
ST - Bio-functional hydrogel with antibacterial and anti-inflammatory dual
properties to combat with burn wound infection
T2 - Bioengineering and Translational Medicine
TI - Bio-functional hydrogel with antibacterial and anti-inflammatory dual
properties to combat with burn wound infection
85133365479&doi=10.1002%2fbtm2.10373&partnerID=40&md5=203119a3f5d4c4e91de859cf608ce
95b
VL - 8
ID - 5053
ER -
TY - JOUR
AB - In this study, an eco-friendly and low-cost procedure for the in situ
fabrication of Cu nanoparticles by using chitosan/alginate hydrogel. The prepared
Cu NPs@CS/Alg nanocomposite were characterized by advanced physicochemical
techniques like Fourier Transformed Infrared spectroscopy (FT-IR), Scanning
Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy
Dispersive X-ray spectroscopy (EDX) and X-ray Diffraction (XRD) study. It has been
established that chitosan/alginate-capped gold nanoparticles have a spherical shape
with a mean diameter from 10 to 20 nm. In the cellular and molecular part of the
recent study, the treated cells with Cu NPs@CS/Alg nanocomposite were assessed by
MTT assay for 48 h about the cytotoxicity and anti-human breast cancer properties
on normal (HUVEC) and breast cancer cell lines i.e. infiltrating lobular carcinoma
of breast (UACC-3133), inflammatory carcinoma of the breast (UACC-732), and
metastatic carcinoma (MDA-MB-453). In the antioxidant test, the IC50 of Cu
NPs@CS/Alg nanocomposite and BHT against DPPH free radicals were 344 and 193 mu
g/mL, respectively. The IC50 of Cu NPs@CS/Alg nanocomposite were 297, 386, and 359
mu g/ mL against KYSE-270, OE33, and ESO26 cell lines, respectively. The viability
of malignant breast cell line reduced dose-dependently in the presence of Cu
NPs@CS/Alg nanocomposite. (C) 2021 Published by Elsevier B.V. on behalf of King
Saud University.
AN - WOS:000745075100005
AU - Xu, D.
AU - Li, E.
AU - Karmakar, B.
AU - Awwad, N. S.
AU - Ibrahium, H. A.
AU - Osman, H. E. H.
AU - El-kott, A. F.
AU - Abdel-Daim, M. M.
C6 - JAN 2022
C7 - 103638
DA - MAR
DO - 10.1016/j.arabjc.2021.103638
IS - 3
PY - 2022
SN - 1878-5352
1878-5379
ST - Green preparation of copper nanoparticle-loaded chitosan/alginate bio-
composite: Investigation of its cytotoxicity, antioxidant and anti-human breast
cancer properties
TI - Green preparation of copper nanoparticle-loaded chitosan/alginate bio-
composite: Investigation of its cytotoxicity, antioxidant and anti-human breast
cancer properties
VL - 15
ID - 6541
ER -
TY - JOUR
AB - AIM: To investigate effect of cytokines in the immunopathogenesis of
Helicobacter pylori (H. pylori). METHODS: Forty-eight patients (37 with chronic
gastritis and 11 with duodenal ulcer (DU), and aged 21-63 yrs) were biopsied under
endoscopy for gastric mucosa culture in vitro and H. pylori detection. Rapid urease
test, H. pylori culture and Warthin-Starry silver stains were used for H. pylori
diagnosis. H. pylori was defined as positive when any 2 of these tests were
positive. Each of 3 antrum pylori biopsies was cultured in vitro for 24 h by adding
1mL RPMI 1640 (37°C, 50mL·L-1 CO2). Supernatants were centrifuged and used for the
detection of interleukin (IL)-6, IL-8 and tumor necrosis factor-a (TNF-α) by ELISA.
Total proteins of the biopsies were assayed by a modified Lowry method. Another 8
cases were cultured with RPMI 1640 added by H. pylori vacuolating cytotoxin (VacA).
The results were expressed as ng orμg per gram protein (ng·g-1 or μg·g-1). RESULTS:
Sixty-nine percent of the patients were H. pylori positive. IL-6, IL-8 and TNF-α
contents in the supernatants of cultured mucosa were all significantly higher in H.
pylori positive patients than those in H. pylori negative patients. Among them, IL-
8 content of 9 cases of DU patients was 53 (18-96) μg·g-1, which was significantly
higher than 36(7-84) μg·g-1 of chronic gastritis patients (P < 0.01). Addition of
VacA into culture media could significantly promote IL-8 secretion from gastric
mucosa (50 ± 38 μg·g-1 vs 68 ± 30 μg·g-1, P < 0.01). TNF-α content was not
significantly increased, and IL-6 was not changed. IL-8 was closely associated with
the inflammation grading and activity (r = 0.98, P < 0.0025). No correlation was
observed between IL-6, TNFα and inflammation grading (r = -0.26 and -0.28, P >
0.25), but they were higher in active gastritis than those in nonactive gastritis.
CONCLUSIONS: IL-8 is closely associated with the infiltration of inflammatory
cells, and H. pylori cytotoxins are responsible for the increased secretion of
cytokines.
AU - Xu, K. Q.
AU - Zhang, W. D.
AU - Wang, J. D.
AU - Li, Z. X.
AU - Zhou, D. Y.
AU - Zhang, Y. L.
AU - Huang, W. F.
AU - Jiang, B.
AU - Sun, Y.
DB - Scopus
IS - 8
KW - cytokine
cytotoxin
gene product
interleukin 6
interleukin 8
unclassified drug
vacuolating toxin
adult
article
biopsy technique
cancer grading
chronic gastritis
clinical article
cytokine production
cytotoxicity
duodenum ulcer
female
Helicobacter pylori
human
immunopathogenesis
male
protein content
staining
stomach antrum
stomach mucosa
M3 - Article
PY - 2002
SP - 907-911
ST - Cytotoxin of Helicobacter pylori promotes IL-8 secretion of gastric mucosa in
vitro
T2 - World Chinese Journal of Digestology
TI - Cytotoxin of Helicobacter pylori promotes IL-8 secretion of gastric mucosa in
vitro
0036689431&partnerID=40&md5=91e06f2b31a0871b8e35b55453322e52
VL - 10
ID - 5788
ER -
TY - JOUR
AB - Background: Since silver-nanoparticles (NPs) possess an antibacterial
activity, they were commonly used in medical products and devices, food storage
materials, cosmetics, various health care products, and industrial products.
Various silver-NP based medical devices are available for clinical uses, such as
silver-NP based dressing and silver-NP based hydrogel (silver-NP-hydrogel) for
medical applications. Although the previous data have suggested silver-NPs induced
toxicity in vivo and in vitro, there is lack information about the mechanisms of
biological response and potential toxicity of silver-NP-hydrogel. Methods: In this
study, the genotoxicity of silver-NP-hydrogel was assayed using cytokinesis-block
micronucleus (CBMN). The molecular response was studied using DNA microarray and GO
pathway analysis. Results and discussion: The results of global gene expression
analysis in HeLa cells showed that thousands of genes were up-or down-regulated at
48 h of silver-NP-hydrogel exposure. Further GO pathway analysis suggested that
fourteen theoretical activating signaling pathways were attributed to up-regulated
genes; and three signal pathways were attributed to down-regulated genes. It was
discussed that the cells protect themselves against silver NP-mediated toxicity
through up-regulating metallothionein genes and anti-oxidative stress genes. The
changes in DNA damage, apoptosis and mitosis pathway were closely related to
silver-NP-induced cytotoxicity and chromosome damage. The down-regulation of CDC14A
via mitosis pathway might play a role in potential genotoxicity induced by silver-
NPs. Conclusions: The silver-NP-hydrogel induced micronuclei formation in cellular
level and broad spectrum molecular responses in gene expression level. The results
of signal pathway analysis suggested that the balances between anti-ROS response
and DNA damage, chromosome instability, mitosis inhibition might play important
roles in silver-NP induced toxicity. The inflammatory factors were likely involved
in silver-NP-hydrogel complex-induced toxic effects via JAK-STAT signal
transduction pathway and immune response pathway. These biological responses
eventually decide the future of the cells, survival or apoptosis.
AN - WOS:000308183400001
AU - Xu, L. M.
AU - Li, X. F.
AU - Takemura, T.
AU - Hanagata, N.
AU - Wu, G.
AU - Chou, L. S. L.
C7 - 16
DA - MAY 1
DO - 10.1186/1477-3155-10-16
PY - 2012
SN - 1477-3155
ST - Genotoxicity and molecular response of silver nanoparticle (NP)-based
hydrogel
TI - Genotoxicity and molecular response of silver nanoparticle (NP)-based
hydrogel
VL - 10
ID - 6113
ER -
TY - JOUR
AB - It is known that the biological half-life of silver in the central nervous
system is longer than in other organs. However, the potential toxicity of silver
nanoparticles (NPs) on brain tissue and the underlying mechanism(s) of action are
not well understood. In this study, neurotoxicity of silver NPs was examined in rat
after intragastric administration. After a two-week exposure to low-dose (1 mg/kg,
body weight) or high-dose (10 mg/kg) silver NPs, the pathological and
ultrastructural changes in brain tissue were evaluated with H&E staining and
transmission electron microscopy. The mRNA expression levels of key tight junction
proteins of the blood brain barrier (BBB) were analyzed by real-time RT-PCR, and
several inflammatory factors were assessed in blood using ELISA assay. We observed
neuron shrinkage, cytoplasmic or foot swelling of astrocytes, and extravascular
lymphocytes in silver NP exposure groups. The cadherin 1 (2(-Delta Delta Ct): 1.45-
fold/control) and Claudin-1 (2(-Delta Delta Ct): 2.77-fold/control) were slightly
increase in mRNA expression levels, and IL-4 significantly increased after silver
NP exposure. It was suggest that silver NP can induce neuronal degeneration and
astrocyte swelling, even with a low-dose (1 mg/kg) oral exposure. One potential
mechanism for the effects of silver NPs to the nervous cells is involved in
inflammatory effects.
AN - WOS:000347435300026
AU - Xu, L. M.
AU - Shao, A. L.
AU - Zhao, Y. H.
AU - Wang, Z. J.
AU - Zhang, C. P.
AU - Sun, Y. L.
AU - Deng, J.
AU - Chou, L. S. L.
DA - JUN
DO - 10.1166/jnn.2015.9612
IS - 6
PY - 2015
SN - 1533-4880
1533-4899
SP - 4215-4223
ST - Neurotoxicity of Silver Nanoparticles in Rat Brain After Intragastric
Exposure
TI - Neurotoxicity of Silver Nanoparticles in Rat Brain After Intragastric
Exposure
VL - 15
ID - 5957
ER -
TY - JOUR
AB - After exposing rat embryonic cells to 20 mg/mL of silver nanoparticle (NP)
suspension and their released ions for different time periods, silver nanoparticles
were found in cellular nuclei, mitochondria, cytoplasm and lysosomes by
transmission electron microscopy (TEM). We also observed Global gene expression
analysis showed a total of 279 genes that were up-regulated and 389 genes that were
down-regulated in the silver-NP suspension exposure group, while 3 genes were up-
regulated and 41 genes were down-regulated in the silver ion exposure group.
Further, the GO pathway analysis suggested that these differentially expressed
genes are involved in several biological processes, such as energy metabolism,
oxygen transport, enzyme activities, molecular binding, etc. It is possible that
inhibition of oxygen transport is mediated by the significant down-regulation of
genes of the globin family, which might play an important role in silver ion-
induced toxicity. KEGG pathway analysis showed that there were 23 signal pathways
that were affected in the cells after exposure to silver-NP suspension, but not
silver ion alone. The most significant change concerned inflammatory signal
pathways, which were only found in silver-NP suspension exposed cells, indicating
that inflammatory response might play an important role in the mechanism(s) of
silver-NP-induced toxicity. The significant up-regulation of matrix
metalloproteinases 3 and 9 suggests that silver NPs could induce extracellular
matrix degradation via an inflammatory signaling pathway. The significant up-
regulation of secretory leukocyte peptidase inhibitor and serine protease inhibitor
2c was considered to be an embryonic cellular defense mechanism in response to
silver-NP-induced inflammation.
AN - WOS:000361329100012
AU - Xu, L. M.
AU - Shi, C.
AU - Shao, A. L.
AU - Li, X. F.
AU - Cheng, X.
AU - Ding, R. G.
AU - Wu, G.
AU - Chou, L. L.
DO - 10.3109/17435390.2014.948942
IS - 4
PY - 2015
SN - 1743-5390
1743-5404
SP - 513-522
ST - Toxic responses in rat embryonic cells to silver nanoparticles and released
silver ions as analyzed via gene expression profiles and transmission electron
microscopy
T2 - NANOTOXICOLOGY
TI - Toxic responses in rat embryonic cells to silver nanoparticles and released
microscopy
VL - 9
ID - 5905
ER -
TY - JOUR
AB - Background: Since silver-nanoparticles (NPs) possess an antibacterial
activity, they were commonly used in medical products and devices, food storage
materials, cosmetics, various health care products, and industrial products.
Various silver-NP based medical devices are available for clinical uses, such as
silver-NP based dressing and silver-NP based hydrogel (silver-NP-hydrogel) for
medical applications. Although the previous data have suggested silver-NPs induced
toxicity in vivo and in vitro, there is lack information about the mechanisms of
biological response and potential toxicity of silver-NP-hydrogel.Methods: In this
study, the genotoxicity of silver-NP-hydrogel was assayed using cytokinesis-block
micronucleus (CBMN). The molecular response was studied using DNA microarray and GO
pathway analysis.Results and discussion: The results of global gene expression
analysis in HeLa cells showed that thousands of genes were up- or down-regulated at
48 h of silver-NP-hydrogel exposure. Further GO pathway analysis suggested that
fourteen theoretical activating signaling pathways were attributed to up-regulated
genes; and three signal pathways were attributed to down-regulated genes. It was
discussed that the cells protect themselves against silver NP-mediated toxicity
through up-regulating metallothionein genes and anti-oxidative stress genes. The
changes in DNA damage, apoptosis and mitosis pathway were closely related to
silver-NP-induced cytotoxicity and chromosome damage. The down-regulation of CDC14A
via mitosis pathway might play a role in potential genotoxicity induced by silver-
NPs.Conclusions: The silver-NP-hydrogel induced micronuclei formation in cellular
level and broad spectrum molecular responses in gene expression level. The results
of signal pathway analysis suggested that the balances between anti-ROS response
and DNA damage, chromosome instability, mitosis inhibition might play important
roles in silver-NP induced toxicity. The inflammatory factors were likely involved
in silver-NP-hydrogel complex-induced toxic effects via JAK-STAT signal
transduction pathway and immune response pathway. These biological responses
eventually decide the future of the cells, survival or apoptosis. © 2012 Xu et al.;
AU - Xu, L.
AU - Li, X.
AU - Takemura, T.
AU - Hanagata, N.
AU - Wu, G.
AU - Chou, L. L.
C7 - 16
DB - Scopus
DO - 10.1186/1477-3155-10-16
KW - Apoptosis and mitosis pathway
DNA damage
Genotoxicity
Global gene expression
JAK-STAT signal transduction pathway
Silver nanoparticle-based hydrogel (silver-NP-hydrogel)
Cell Shape
Down-Regulation
HeLa Cells
Humans
Hydrogel
Metal Nanoparticles
Micronucleus Tests
Models, Biological
Mutagenicity Tests
Mutagens
Oligonucleotide Array Sequence Analysis
Silver
Up-Regulation
Cell death
Chromosomes
DNA
Food storage
Gene expression
Health care
Hydrogels
Nanoparticles
Signal transduction
Toxicity
silver nanoparticle
Anti-oxidative stress
Biological response
Broad spectrum
Cellular levels
Clinical use
DNA damages
DNA micro-array
Down-regulation
Gene expression analysis
Gene expression levels
Genotoxicities
Health care products
HeLa cell
Immune response
In-vitro
In-vivo
Industrial product
Medical Devices
Medical products
Metallothioneins
Molecular response
Pathway analysis
Signal pathways
Signal transduction pathways
Signaling pathways
Silver-nanoparticles
Toxic effect
apoptosis
article
cell structure
chromosome damage
cytokinesis
DNA microarray
down regulation
gene
gene control
gene expression
genotoxicity
human
human cell
hydrogel
metallothionein gene
micronucleus test
mitosis
molecular dynamics
risk assessment
toxicity testing
upregulation
M3 - Article
PY - 2012
ST - Genotoxicity and molecular response of silver nanoparticle (NP)-based
hydrogel
TI - Genotoxicity and molecular response of silver nanoparticle (NP)-based
hydrogel
84862180734&doi=10.1186%2f1477-3155-10-
16&partnerID=40&md5=f23b0216faa5a66dc5e5e5816bde7854
VL - 10
ID - 5742
ER -
TY - JOUR
AB - It is known that the biological half-life of silver in the central nervous
system is longer than in other organs. However, the potential toxicity of silver
nanoparticles (NPs) on brain tissue and the underlying mechanism(s) of action are
not well understood. In this study, neurotoxicity of silver NPs was examined in rat
after intragastric administration. After a two-week exposure to low-dose (1 mg/kg,
body weight) or high-dose (10 mg/kg) silver NPs, the pathological and
ultrastructural changes in brain tissue were evaluated with H&E staining and
transmission electron microscopy. The mRNA expression levels of key tight junction
proteins of the blood-brain barrier (BBB) were analyzed by real-time RT-PCR, and
several inflammatory factors were assessed in blood using ELISA assay. We observed
neuron shrinkage, cytoplasmic or foot swelling of astrocytes, and extra-vascular
lymphocytes in silver NP exposure groups. The cadherin 1 (2 -ΔΔCt:
1.45-fold/control) and Claudin-1 (2 -ΔΔCt: 2.77-fold/control) were slightly
increase in mRNA expression levels, and IL-4 significantly increased after silver
NP exposure. It was suggest that silver NP can induce neuronal degeneration and
astrocyte swelling, even with a low-dose (1 mg/kg) oral exposure. One potential
mechanism for the effects of silver NPs to the nervous cells is involved in
inflammatory effects. Copyright © 2015 American Scientific Publishers
AU - Xu, L.
AU - Shao, A.
AU - Zhao, Y.
AU - Wang, Z.
AU - Zhang, C.
AU - Sun, Y.
AU - Deng, J.
AU - Chou, L. L.
C7 - A26
DB - Scopus
DO - 10.1166/jnn.2015.9612
IS - 6
KW - Inflammatory effects
Neurotoxicity
Silver NPs
Tight junction proteins
Ultrastructural changes
Animals
Brain
Brain Chemistry
Female
Metal Nanoparticles
Rats
Silver
Tight Junction Proteins
Biological organs
Blood
Nanoparticles
Neurons
Proteins
Tissue
Toxicity
metal nanoparticle
silver
Central nervous systems
Intragastric administration
MRNA expression level
Silver nanoparticle (NPs)
Tight junctions
animal
blood
brain
brain chemistry
chemistry
cytology
drug effects
female
metabolism
pathology
rat
Sprague Dawley rat
M3 - Article
PY - 2015
SP - 4215-4223
ST - Neurotoxicity of silver nanoparticles in rat brain after intragastric
exposure
TI - Neurotoxicity of silver nanoparticles in rat brain after intragastric
exposure
84920847630&doi=10.1166%2fjnn.2015.9612&partnerID=40&md5=16dfc279e5e07b30feba6e4d1c
91f9f9
VL - 15
ID - 5625
ER -
TY - JOUR
AB - After exposing rat embryonic cells to 20μg/mL of silver nanoparticle (NP)
suspension and their released ions for different time periods, silver nanoparticles
were found in cellular nuclei, mitochondria, cytoplasm and lysosomes by
transmission electron microscopy (TEM). We also observed mitochondrial destruction,
distension of endoplasmic reticulum and apoptotic bodies. Global gene expression
analysis showed a total of 279 genes that were up-regulated and 389 genes that were
down-regulated in the silver-NP suspension exposure group, while 3 genes were up-
regulated and 41 genes were down-regulated in the silver ion exposure group.
Further, the GO pathway analysis suggested that these differentially expressed
genes are involved in several biological processes, such as energy metabolism,
oxygen transport, enzyme activities, molecular binding, etc. It is possible that
inhibition of oxygen transport is mediated by the significant down-regulation of
genes of the globin family, which might play an important role in silver ion-
induced toxicity. KEGG pathway analysis showed that there were 23 signal pathways
that were affected in the cells after exposure to silver-NP suspension, but not
silver ion alone. The most significant change concerned inflammatory signal
pathways, which were only found in silver-NP suspension exposed cells, indicating
that inflammatory response might play an important role in the mechanism(s) of
silver-NP-induced toxicity. The significant up-regulation of matrix
metalloproteinases 3 and 9 suggests that silver NPs could induce extracellular
matrix degradation via an inflammatory signaling pathway. The significant up-
regulation of secretory leukocyte peptidase inhibitor and serine protease inhibitor
2c was considered to be an embryonic cellular defense mechanism in response to
silver-NP-induced inflammation. © 2014 Informa UK Ltd. All rights reserved:
reproduction in whole or part not permitted.
AU - Xu, L.
AU - Shi, C.
AU - Shao, A.
AU - Li, X.
AU - Cheng, X.
AU - Ding, R.
AU - Wu, G.
AU - Chou, L. L.
DB - Scopus
DO - 10.3109/17435390.2014.948942
IS - 4
KW - Embryonic cells
Global gene expression
Inflammatory effects
Ultrastructural damages
Animals
Embryo, Mammalian
Metal Nanoparticles
Rats
Silver
Rattus
gelatinase B
silver
silver nanoparticle
stromelysin
metal nanoparticle
animal cell
animal experiment
apoptosis
Article
cell nucleus
controlled study
cytoplasm
cytotoxicity
DNA microarray
down regulation
embryo
embryo cell
energy metabolism
enzyme activity
gene expression
lysosome
mitochondrion
nonhuman
oxygen transport
priority journal
rat
upregulation
animal
animal embryo
chemistry
drug effects
M3 - Article
PY - 2015
SP - 513-522
ST - Toxic responses in rat embryonic cells to silver nanoparticles and released
microscopy
T2 - Nanotoxicology
TI - Toxic responses in rat embryonic cells to silver nanoparticles and released
microscopy
84929758411&doi=10.3109%2f17435390.2014.948942&partnerID=40&md5=9ca61480e7ae8392b28
2e112dc01c098
VL - 9
ID - 5641
ER -
TY - JOUR
AB - Reactive oxygen species (ROS) produced by noble metallic nanoparticles under
visible light is an effective way to combat drug-resistant bacteria colonized on
the wound. However, the photocatalytic efficiency of noble metallic nanoparticles
is limited by its self-aggregation in water media. Moreover, the fast release of
noble metallic ions from nanoparticles might engender cellular toxicity and
hazardous environmental issues. Herein, we chose AgNPs, the most common plasmonic
noble metallic nanoparticles, as an example, modifying the surface of AgNPs with
oleic acid and n-butylamine and imbedded them into calcium alginate (CA) hydrogel
that holds tissue adhesion, rapid hemostatic, sunlight -sensitive antibacterial and
anti-inflammatory abilities, and thus effectively promotes the healing of wounds.
Unlike conventional AgNP-based materials, the constrain of colloids and hydrogel
networks hinders the leach of Ag+. Nonetheless, the CA/Ag hydrogels exhibit on-
demand photodynamic antibacterial efficacy due to the generation of ROS under
visible light. In addition, the CA/Ag hydrogel can effectively stop the hemorrhage
in a mouse liver bleeding model due to their skin-adaptive flexibility and tissue
adhesiveness. The potent sunlight-responsive antibacterial activity of the CA/Ag
hydrogel can effectively kill multidrug-resistant bacteria both in vitro (>99.999%)
and in vivo (>99.9%), while the diminished Ag+ release guarantees its
biocompatibility. The CA/Ag hydrogel significantly promotes the wound healing
process by the downregulation of proinflammatory cytokines (TNF-alpha and IL-6) in
a rodent full-thickness cutaneous wound model. Overall, the proposed
multifunctional CA/Ag nanocomposite hydrogel has excellent prospects as an advanced
wound dressing.
AN - WOS:000971593100001
AU - Xu, M.
AU - Ji, X. H.
AU - Huo, J. J.
AU - Chen, J. J.
AU - Liu, N.
AU - Li, Z. Y.
AU - Jia, Q. Y.
AU - Sun, B.
AU - Zhu, M. F.
AU - Li, P.
DA - APR 12
DO - 10.1021/acsami.3c03247
IS - 14
PY - 2023
SN - 1944-8244
1944-8252
SP - 17742-17756
ST - Nonreleasing AgNP Colloids Composite Hydrogel with Potent Hemostatic,
Photodynamic Bactericidal and Wound Healing- Promoting Properties
TI - Nonreleasing AgNP Colloids Composite Hydrogel with Potent Hemostatic,
Photodynamic Bactericidal and Wound Healing- Promoting Properties
VL - 15
ID - 6585
ER -
TY - JOUR
AB - Open wounds (e.g., burns and trauma) are always challenged by various
opportunistic bacteria. There is an urgent need for developing novel wound dressing
that is able to prevent bacterial infection and promote the healing simultaneously.
Herein, we developed a new type of antimicrobial hydrogels for the open wound
healing through imitating a facile mussel-inspired catechol/polyamine chemistry.
This hydrogel was prepared using catechol (CT) and e-poly-L-lysine (EPL) by
oxidative cross-linking directly in the open air at room temperature. This
nonleaching CT/EPL hydrogel not only exhibited excellent contact-active
antimicrobial activities against Gram-negative bacteria Escherichia coli (E. coli)
and Gram-positive "superbug" methicillin-resistant Staphylococcus aureus (MRSA) but
also inhibited the biofilm formation in vitro. Moreover, the animal burn wound
model study clearly validated the in vivo anti-infective property of CT/EPL
hydrogel against MRSA infection. More importantly, the CT/EPL hydrogel possessed
low cytotoxicity and enhanced cell migration in vitro. A full-thickness cutaneous
wound model study revealed that CT/EPL hydrogel upregulated the expression of
vascular endothelial growth factor (VEGF) and reduced the production of the pro-
inflammatory cytokines, thus promoted the wound healing. These findings suggested
that the CT/EPL hydrogel have great potential as a wound dressing for preventing
bacterial infection and accelerating healing of open wounds.
AN - WOS:000616371100022
AU - Xu, M.
AU - Khan, A.
AU - Wang, T. J.
AU - Song, Q.
AU - Han, C. M.
AU - Wang, Q. Q.
AU - Gao, L. L.
AU - Huang, X.
AU - Li, P.
AU - Huang, W.
DA - AUG 19
DO - 10.1021/acsabm.9b00353
IS - 8
PY - 2019
SN - 2576-6422
SP - 3329-3340
ST - Mussel-Inspired Hydrogel with Potent in Vivo Contact-Active Antimicrobial and
Wound Healing Promoting Activities
T2 - ACS APPLIED BIO MATERIALS
TI - Mussel-Inspired Hydrogel with Potent in Vivo Contact-Active Antimicrobial and
Wound Healing Promoting Activities
VL - 2
ID - 6790
ER -
TY - JOUR
AB - Background Most traditional wound dressings only partially meet the needs of
wound healing because of their single function. Patients usually suffer from the
increasing cost of treatment and pain resulting from the frequent changing of wound
dressings. Herein, we have developed a mutifunctional cryogel to promote bacterial
infected wound healing based on a biocompatible polysaccharide. Methods The
multifunctional cryogel is made up of a compositive scaffold of chitosan (CS),
gelatin (Gel) and tannic acid (TA) and in situ formed silver nanoparticles (Ag
NPs). A liver bleeding rat model was used to evaluate the dynamic hemostasis
performance of the various cryogels. In order to evaluate the antibacterial
properties of the prepared cryogels, gram-positive bacterium Staphylococcus aureus
(S. aureus) and gram-negative bacterium Escherichia coli (E. coli) were cultured
with the cryogels for 12 h. Meanwhile, S. aureus was introduced to cause bacterial
infection in vivo. After treatment for 2 days, the exudates from wound sites were
dipped for bacterial colony culture. Subsequently, the anti-inflammatory effect of
the various cryogels was evaluated by western blotting and enzyme-linked
immunosorbent assay. Finally, full-thickness skin defect models on the back of SD
rats were established to assess the wound healing performances of the cryogels.
Results Due to its porous structure, the multifunctional cryogel showed fast liver
hemostasis. The introduced Ag NPs endowed the cryogel with an antibacterial
efficiency of >99.9% against both S. aureus and E. coli. Benefited from the
polyphenol groups of TA, the cryogel could inhibit nuclear factor-kappa B nuclear
translocation and down-regulate inflammatory cytokines for an anti-inflammatory
effect. Meanwhile, excessive reactive oxygen species could also be scavenged
effectively. Despite the presence of Ag NPs, the cryogel did not show cytotoxicity
and hemolysis. Moreover, in vivo experiments demonstrated that the biocompatible
cryogel displayed effective bacterial disinfection and accelerated wound healing.
Conclusions The multifunctional cryogel, with fast hemostasis, antibacterial and
anti-inflammation properties and the ability to promote cell proliferation could be
widely applied as a wound dressing for bacterial infected wound healing.
AN - WOS:000836990900001
AU - Xu, N.
AU - Yuan, Y. C.
AU - Ding, L. P.
AU - Li, J. F.
AU - Jia, J. Z.
AU - Li, Z.
AU - He, D. F.
AU - Yu, Y. L.
C7 - tkac019
DA - JAN 1
DO - 10.1093/burnst/tkac019
PY - 2022
SN - 2321-3868
2321-3876
ST - Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ
reduced silver nanoparticles for wound healing
T2 - BURNS & TRAUMA
TI - Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ
VL - 10
ID - 6143
ER -
TY - JOUR
AB - Background: Most traditional wound dressings only partially meet the needs of
wound healing because of their single function. Patients usually suffer from the
increasing cost of treatment and pain resulting from the frequent changing of wound
dressings. Herein, we have developed a mutifunctional cryogel to promote bacterial
infected wound healing based on a biocompatible polysaccharide. Methods: The
multifunctional cryogel is made up of a compositive scaffold of chitosan (CS),
gelatin (Gel) and tannic acid (TA) and in situ formed silver nanoparticles (Ag
NPs). A liver bleeding rat model was used to evaluate the dynamic hemostasis
performance of the various cryogels. In order to evaluate the antibacterial
properties of the prepared cryogels, gram-positive bacterium Staphylococcus aureus
(S. aureus) and gram-negative bacterium Escherichia coli (E. coli) were cultured
with the cryogels for 12 h. Meanwhile, S. aureus was introduced to cause bacterial
infection in vivo. After treatment for 2 days, the exudates from wound sites were
dipped for bacterial colony culture. Subsequently, the anti-inflammatory effect of
the various cryogels was evaluated by western blotting and enzyme-linked
immunosorbent assay. Finally, full-Thickness skin defect models on the back of SD
rats were established to assess the wound healing performances of the cryogels.
Results: Due to its porous structure, the multifunctional cryogel showed fast liver
hemostasis. The introduced Ag NPs endowed the cryogel with an antibacterial
efficiency of >99.9% against both S. aureus and E. coli. Benefited from the
polyphenol groups of TA, the cryogel could inhibit nuclear factor-κB nuclear
translocation and down-regulate inflammatory cytokines for an anti-inflammatory
effect. Meanwhile, excessive reactive oxygen species could also be scavenged
effectively. Despite the presence of Ag NPs, the cryogel did not show cytotoxicity
and hemolysis. Moreover, in vivo experiments demonstrated that the biocompatible
cryogel displayed effective bacterial disinfection and accelerated wound healing.
Conclusions: The multifunctional cryogel, with fast hemostasis, antibacterial and
anti-inflammation properties and the ability to promote cell proliferation could be
widely applied as a wound dressing for bacterial infected wound healing. © 2022 The
Author(s). Published by Oxford University Press.
AU - Xu, N.
AU - Yuan, Y.
AU - Ding, L.
AU - Li, J.
AU - Jia, J.
AU - Li, Z.
AU - He, D.
AU - Yu, Y.
C7 - tkac019
DB - Scopus
DO - 10.1093/burnst/tkac019
KW - Anti-inflammation
Antibacterial
Bacterial infection
Cryogel
Hemostasis
Wound dressing
Wound healing
chitosan
cryogel
gelatin
silver nanoparticle
tannin
animal cell
animal experiment
animal model
animal tissue
Article
bacterial infection
bacterium colony
bacterium culture
biocompatibility
controlled study
down regulation
drug cytotoxicity
Escherichia coli
hemostasis
in vivo study
liver hemorrhage
mouse
nanofabrication
nonhuman
skin defect
Western blotting
wound fluid
wound healing
wound infection
M3 - Article
PY - 2022
ST - Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ
T2 - Burns and Trauma
TI - Multifunctional chitosan/gelatin@tannic acid cryogels decorated with in situ
85136145561&doi=10.1093%2fburnst
%2ftkac019&partnerID=40&md5=63126451310eaed9ce637a3cbe47f7b8
VL - 10
ID - 5082
ER -
TY - JOUR
AB - As the continuous development of the industrial revolution, nanomaterials
with excellent characteristics have been widely applied in various fields, greatly
increasing the probability of human exposure to nanomaterials and the concerns
about the potential nanotoxicity. Existing studies have shown that the toxicity of
nanomaterials may be closely related to oxidative stress, inflammatory response,
phagocytosis dysfunction, DNA damage, etc. Based on our focus, nanomaterials may
cross the human barrier through various channels and disrupt various cell-cell
junctions, while the integrity of cellular barrier is a necessary for the normal
physiological function of various organs. However, until now, there is still a lack
of systematic discussion in this field. This review illustrates the importance of
cell-cell junctions in maintaining various organ functions and highlights the
mechanism of various nanomaterials disrupt cell-cell junctions, as well as the
possible damage to various organs, such as brain, eye, lung, breast, intestine,
placenta, testis, heart, liver, kidney, skin, etc. Awareness of the potential
negative effects of nanomaterials will help scientists deeply understand the
limitations of nanotechnology, inspiring them to develop safer and more efficient
nanomaterials for future personalized nanomedicine.
AN - WOS:000949632500001
AU - Xu, S. B.
AU - Pang, X. L.
AU - Zhang, X. Y.
AU - Lv, Q.
AU - Zhang, M.
AU - Wang, J. P.
AU - Ni, N. Y.
AU - Sun, X.
C6 - MAR 2023
DA - 2023 MAR 16
DO - 10.1007/s12274-023-5455-y
PY - 2023
SN - 1998-0124
1998-0000
ST - Microenvironment regulation of M-N-C single-atom catalysts towards oxygen
reduction reaction
T2 - NANO RESEARCH
TI - Microenvironment regulation of M-N-C single-atom catalysts towards oxygen
reduction reaction
ID - 6658
ER -
TY - JOUR
AB - As the continuous development of the industrial revolution, nanomaterials
with excellent characteristics have been widely applied in various fields, greatly
increasing the probability of human exposure to nanomaterials and the concerns
about the potential nanotoxicity. Existing studies have shown that the toxicity of
nanomaterials may be closely related to oxidative stress, inflammatory response,
phagocytosis dysfunction, DNA damage, etc. Based on our focus, nanomaterials may
cross the human barrier through various channels and disrupt various cell-cell
junctions, while the integrity of cellular barrier is a necessary for the normal
physiological function of various organs. However, until now, there is still a lack
of systematic discussion in this field. This review illustrates the importance of
cell -cell junctions in maintaining various organ functions and highlights the
mechanism of various nanomaterials disrupt cell -cell junctions, as well as the
possible damage to various organs, such as brain, eye, lung, breast, intestine,
placenta, testis, heart, liver, kidney, skin, etc. Awareness of the potential
negative effects of nanomaterials will help scientists deeply understand the
limitations of nanotechnology, inspiring them to develop safer and more efficient
nanomaterials for future personalized nanomedicine.
AN - WOS:001023725400099
AU - Xu, S. B.
AU - Pang, X. L.
AU - Zhang, X. Y.
AU - Lv, Q.
AU - Zhang, M.
AU - Wang, J. P.
AU - Ni, N. Y.
AU - Sun, X.
DA - MAY
DO - 10.1007/s12274-023-5455-y
IS - 5
PY - 2023
SN - 1998-0124
1998-0000
SP - 7053-7074
ST - Nanomaterials diseases cell -cell junctions towards various diseases
T2 - NANO RESEARCH
TI - Nanomaterials diseases cell -cell junctions towards various diseases
VL - 16
ID - 6461
ER -
TY - JOUR
AB - Nanoparticles (NPs) have become one of the most popular objects of scientific
study during the past decades. However, despite wealth of study reports, still
there is a gap, particularly in health toxicology studies, underlying mechanisms,
and related evaluation models to deeply understanding the NPs risk effects. In this
review, we first present a comprehensive landscape of the applications of NPs on
health, especially addressing the role of NPs in medical diagnosis, therapy. Then,
the toxicity of NPs on health systems is introduced. We describe in detail the
effects of NPs on various systems, including respiratory, nervous, endocrine,
immune, and reproductive systems, and the carcinogenicity of NPs. Furthermore, we
unravels the underlying mechanisms of NPs including ROS accumulation, mitochondrial
damage, inflammatory reaction, apoptosis, DNA damage, cell cycle, and epigenetic
regulation. In addition, the classical study models such as cell lines and mice and
the emerging models such as 3D organoids used for evaluating the toxicity or
scientific study are both introduced. Overall, this review presents a critical
summary and evaluation of the state of understanding of NPs, giving readers more
better understanding of the NPs toxicology to remedy key gaps in knowledge and
techniques.
AN - WOS:001025193700001
AU - Xuan, L. H.
AU - Ju, Z.
AU - Skonieczna, M.
AU - Zhou, P. K.
AU - Huang, R. X.
C7 - e327
DA - AUG
DO - 10.1002/mco2.327
IS - 4
PY - 2023
SN - 2688-2663
ST - Nanoparticles-induced potential toxicity on human health: Applications,
toxicity mechanisms, and evaluation models
T2 - MEDCOMM
TI - Nanoparticles-induced potential toxicity on human health: Applications,
toxicity mechanisms, and evaluation models
VL - 4
ID - 6325
ER -
TY - JOUR
AB - Objective: To evaluate the biocompatibility of chemically extracted acellular
muscle grafts (CEAM) and to demonstrate its advantages as tissue engineering
scaffold after transplantation into the spinal cords of adult rats. Methods:
Thirty-six male rats were randomly assigned to control group and CEAM group, and
underwent spinal cord lateral hemisection. After preparation of successful models,
treatment consisted of application of CEAM into the lesion gap, which was left
empty in control group. Six rats in each group were killed 1, 2 and 4 weeks after
induction of the injury. Sections were stained for quantification of
microglia/macrophages using ED-1 on week 1, week 2 and week 4. Additionally,
Holmes' silver staining method was chosen to detect axonal regeneration, glial
fibrillary acidic protein (GFAP) staining for detection of astrocytes in glial
scars, and alkaline phosphatase staining for vascularisation on day 28. Results: In
control group, no definite axonal outgrowth into the lesion was found. The
inflammatory response was most pronounced on day 7 and a dense non-oriented
accumulation of astrocytes could be found at the edge of the lesion cavity in
control group. In CEAM group, the number of regenerating axons in the scaffolds was
613.17 ±154.96, and they grew into the grafts in a strikingly organized fashion.
The change of inflammatory response in CEAM group was the same as that in control
group and there was no sign of the foreign body reaction induced by CEAM. In rats
of CEAM group the astrocytes grew into the graft in a diffuse linear array.
Vascularisation of the grafts was also confirmed. Conclusion: CEAM is biocompatible
with spinal cord, and it indicates that CEAM is a kind of good tissue engineering
scaffold for the repair of spinal cord injury.
AU - Xue, H.
AU - Chen, D.
AU - Zhang, X. Y.
AU - Liu, Y.
DB - Scopus
IS - 5
KW - Acellular muscle
Rats, Wistar
Spinal cord injury
Tissue engineering
Transplantation
animal cell
animal experiment
article
astrocyte
biocompatibility
control group
controlled study
foreign body
graft perfusion
inflammation
macrophage
male
methodology
microglia
muscle graft
nerve cell growth
nerve fiber degeneration
nerve fiber regeneration
nonhuman
quantitative study
rat
spinal cord hemisection
spinal cord injury
staining
tissue engineering
tissue section
vascularization
M3 - Article
PY - 2009
SP - 801-804
ST - Biocompatibility of chemically extracted acellular muscle grafts as
biomatrices in experimental spinal cord injury in rats
T2 - Journal of Jilin University Medicine Edition
TI - Biocompatibility of chemically extracted acellular muscle grafts as
biomatrices in experimental spinal cord injury in rats
70350741614&partnerID=40&md5=e15b23e7c58bc02ae668786876ef697f
VL - 35
ID - 5804
ER -
TY - JOUR
AB - This study evaluated the acute toxicity and biokinetics of intravenously
administered silver nanoparticles (AgNPs) in mice. Mice were exposed to different
dosages of AgNPs (7.5, 30 or 120?mg kg-1). Toxic effects were assessed via general
behavior, serum biochemical parameters and histopathological observation of the
mice. Biokinetics and tissue distribution of AgNPs were evaluated at a dose of 120?
mg kg-1 in both male and female mice. Inductively coupled plasmamass spectrometry
(ICP-MS) was used to determine silver concentrations in blood and tissue samples
collected at predetermined time intervals. After 2?weeks, AgNPs exerted no obvious
acute toxicity in the mice. However, inflammatory reactions in lung and liver cells
were induced in mice treated at the 120?mg kg-1 dose level. The highest silver
levels were observed in the spleen, followed by liver, lungs and kidneys. The
elimination half-lives and clearance of AgNPs were 15.6?h and 1.0?ml h-1 g-1 for
male mice and 29.9?h and 0.8?ml h-1 g-1 for female mice. These results indicated
that AgNPs could be distributed extensively to various tissues in the body, but
primarily in the spleen and liver. Furthermore, there appears to be gender-related
differences in the biokinetic profiles in blood and distribution in lungs and
kidneys following an intravenous injection of AgNPs. The data from this study
provides information on toxicity and biodistribution of AgNPs following intravenous
administration in mice, which represents the worst case scenario of toxicity among
all the different administration routes, and may shed light in the future use of
products containing AgNPs in humans. Copyright (c) 2012 John Wiley & Sons, Ltd.
AN - WOS:000309190200003
AU - Xue, Y. Y.
AU - Zhang, S. S.
AU - Huang, Y. M.
AU - Zhang, T.
AU - Liu, X. R.
AU - Hu, Y. Y.
AU - Zhang, Z. Y.
AU - Tang, M.
DA - NOV
DO - 10.1002/jat.2742
IS - 11
PY - 2012
SN - 0260-437X
1099-1263
SP - 890-899
ST - Acute toxic effects and gender-related biokinetics of silver nanoparticles
following an intravenous injection in mice
TI - Acute toxic effects and gender-related biokinetics of silver nanoparticles
VL - 32
ID - 5949
ER -
TY - JOUR
AB - This study evaluated the acute toxicity and biokinetics of intravenously
administered silver nanoparticles (AgNPs) in mice. Mice were exposed to different
dosages of AgNPs (7.5, 30 or 120mg kg-1). Toxic effects were assessed via general
behavior, serum biochemical parameters and histopathological observation of the
mice. Biokinetics and tissue distribution of AgNPs were evaluated at a dose of
120mg kg-1 in both male and female mice. Inductively coupled plasma-mass
spectrometry (ICP-MS) was used to determine silver concentrations in blood and
tissue samples collected at predetermined time intervals. After 2weeks, AgNPs
exerted no obvious acute toxicity in the mice. However, inflammatory reactions in
lung and liver cells were induced in mice treated at the 120mg kg-1 dose level. The
highest silver levels were observed in the spleen, followed by liver, lungs and
kidneys. The elimination half-lives and clearance of AgNPs were 15.6h and 1.0ml h-1
g-1 for male mice and 29.9h and 0.8ml h-1 g-1 for female mice. These results
indicated that AgNPs could be distributed extensively to various tissues in the
body, but primarily in the spleen and liver. Furthermore, there appears to be
gender-related differences in the biokinetic profiles in blood and distribution in
lungs and kidneys following an intravenous injection of AgNPs. The data from this
study provides information on toxicity and biodistribution of AgNPs following
intravenous administration in mice, which represents the worst case scenario of
toxicity among all the different administration routes, and may shed light in the
future use of products containing AgNPs in humans. Copyright © 2012 John Wiley &
Sons, Ltd. The acute toxic effects and biokinetics of intravenously administered
silver nanoparticles (AgNPs) were investigated. AgNPs exerted no obvious acute
toxicity when given intravenously in mice at dose levels of 7.5-120mg kg-1. AgNPs
could be distributed extensively to various tissues in the body, and the spleen and
liver were the main target organs. Gender-related differences for the biokinetics
and distribution were noted, and the elimination half-lives were 15.6 and 29.9h for
male and female mice, respectively. © 2012 John Wiley & Sons, Ltd.
AU - Xue, Y.
AU - Zhang, S.
AU - Huang, Y.
AU - Zhang, T.
AU - Liu, X.
AU - Hu, Y.
AU - Zhang, Z.
AU - Tang, M.
DB - Scopus
DO - 10.1002/jat.2742
IS - 11
KW - Acute toxic effects
Gender-related biokinetics
Mice
Tissue distribution
Animals
Female
Injections, Intravenous
Kinetics
Male
Mass Spectrometry
Metal Nanoparticles
Mice, Inbred ICR
Nanoparticles
Sex Factors
Silver
Tissue Distribution
Mus
albumin
cholesterol
creatinine
protein
silver nanoparticle
animal behavior
animal experiment
article
biokinetics
chemical, physical and mathematical phenomena
controlled study
drug blood level
drug tissue level
female
histopathology
inflammation
kidney
liver
liver cell
lung
male
mass spectrometry
mouse
nonhuman
priority journal
sex difference
spleen
M3 - Article
PY - 2012
SP - 890-899
ST - Acute toxic effects and gender-related biokinetics of silver nanoparticles
TI - Acute toxic effects and gender-related biokinetics of silver nanoparticles
84866863235&doi=10.1002%2fjat.2742&partnerID=40&md5=9977b3b848522e950f1e6c286ec0a2e
7
VL - 32
ID - 5752
ER -
TY - JOUR
AB - Diabetes mellitus (Madhumeha) is one of the leading metabolic disorder
prevalent in the developing countries which is characterized by high blood sugar
level and is associated with macrovascular and microvascular complications. The
Indian Ayurveda describes several herbs for the management and treatment of
diabetes mellitus among which Gymnema sylvestre (Asclepiadaceae) is revered as a
potential antidiabetic herbal drug which has the capability of simultaneously
regenerating β-cell and stimulating insulin secretion. Gymnema sylvestre also
possesses anti-obesity, anti-hyperlipidemic, anti-inflammatory, and anti-cancerous
activities. This review updates the recent developments in the experimental studies
conducted on the Gymnema sylvestre as an effective remedy for diabetes mellitus
evidenced by both animals and human studies. Moreover, this study also discussed
the toxicity of Gymnema sylvestre and future challenges in the roadmap of
formulation for prevention and control of diabetes. ©Mattioli 1885.
AU - Yadav, D.
AU - Kwak, M.
AU - Jin, J. O.
DB - Scopus
DO - 10.23751/pn.v21i2.7780
IS - 2
KW - Anti-cancerous
Anti-diabetic
Anti-hyperlipidemic
Anti-inflammatory
Diabetes mellitus
Gymnema sylvestre
alpha tocopherol
antidiabetic agent
ascorbic acid
conduritol
cytokine
dihydroxygymnemic triacetate
glimepiride
glucuronide
glutathione
gold nanoparticle
Gymnema sylvestre extract
gymnemagenin
gymnemasaponin V
gymnemate
gymnemic acid
gymnemoside B
hemoglobin A1c
herbaceous agent
insulin
saponin
silver nanoparticle
triterpene
unclassified drug
adjuvant arthritis
antifungal activity
Ayurveda
Candida albicans
developing country
diabetes mellitus
drug safety
glucose blood level
herb
human
hyperlipidemia
hypolipidemic activity
immunomodulation
insulin release
nonhuman
obesity
oral glucose tolerance test
pancreas islet beta cell
phytochemistry
plant leaf
plant stem
Review
toxicity testing
wound healing
M3 - Review
PY - 2019
SP - 258-269
ST - Clinical applications of Gymnema sylvestre against type 2 diabetes mellitus
and its associated abnormalities
T2 - Progress in Nutrition
TI - Clinical applications of Gymnema sylvestre against type 2 diabetes mellitus
and its associated abnormalities
85069438506&doi=10.23751%2fpn.v21i2.7780&partnerID=40&md5=dbae2cba521a182c2e05eab5b
219aef1
VL - 21
ID - 5407
ER -
TY - JOUR
AB - Process of wound healing is a complex biological process involving different
overlapping phases to attain reepithelialization. Advancement of nanotechnology in
medical sciences has endowed zinc oxide and iron oxide nanoparticles as novel
therapeutic approaches, which potentially promote the process of wound healing.
Current study was designed to explore the effect of biofabricated zinc oxide and
iron oxide nanoparticles of Prosopis cineraria (PC) leaves extract (ZnPC and FePC,
respectively) on dermal full thickness wounds. In vitro antioxidant activity was
performed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and anti-
inflammatory activity was confirmed by albumin denaturation and proteinase
inhibition method. ZnPC and FePC were synthesized by co-precipitation method via
green route and characterized by various techniques. Various parameters of wound
such as wound closure rate, epithelialization period, hydroxyproline content,
tensile strength and level of antioxidant enzyme as well as inflammatory markers
were evaluated. Different analyses exhibited the spherical particles of 117.5
(ZnPC) and 48 (FePC) nm size. Significant effect (p < 0.05) of nano-ointment
topical formulations on wound contraction rate and epithelialization period was
observed. Hydroxyproline content, inflammatory markers and enzymatic antioxidant
profile also supported the wound healing effect. ZnPC ointment applied group showed
quick healing of tissue injury as compared to FePC ointment applied group.
Antioxidant and anti-inflammatory action, due to the synergistic effect of metal
oxides and polyphenolic compounds of PC leaves, could be the possible underlying
mechanism of swift wound healing property of ZnPC and FePC. Therefore, ZnPC and
FePC based nano-ointment approach could be a beneficial way for clinical treatment
of wounds.
AN - WOS:000703697500007
AU - Yadav, E.
AU - Yadav, P.
AU - Verma, A.
C6 - SEP 2021
C7 - 102833
DA - DEC
DO - 10.1016/j.jddst.2021.102833
PY - 2021
SN - 1773-2247
2588-8943
ST - Amelioration of full thickness dermal wounds by topical application of
biofabricated zinc oxide and iron oxide nano-ointment in albino Wistar rats
TI - Amelioration of full thickness dermal wounds by topical application of
biofabricated zinc oxide and iron oxide nano-ointment in albino Wistar rats
VL - 66
ID - 6620
ER -
TY - JOUR
AB - The TRAIL (TNF-related apoptosis-inducing ligand) apoptotic pathway is
extensively exploited in the development of targeted antitumor therapy due to TRAIL
specificity towards its cognate receptors, namely death receptors DR4 and DR5.
Although therapies targeting the TRAIL pathway have encountered many obstacles in
attempts at clinical implementation for cancer treatment, the unique features of
the TRAIL signaling pathway continue to attract the attention of researchers.
Special attention is paid to the design of novel nanoscaled delivery systems,
primarily aimed at increasing the valency of the ligand for improved death receptor
clustering that enhances apoptotic signaling. Optionally, complex nanoformulations
can allow the encapsulation of several therapeutic molecules for a combined
synergistic effect, for example, chemotherapeutic agents or photosensitizers.
Scaffolds for the developed nanodelivery systems are fabricated by a wide range of
conventional clinically approved materials and innovative ones, including metals,
carbon, lipids, polymers, nanogels, protein nanocages, virus-based nanoparticles,
dendrimers, DNA origami nanostructures, and their complex combinations. Most
nanotherapeutics targeting the TRAIL pathway are aimed at tumor therapy and
theranostics. However, given the wide spectrum of action of TRAIL due to its
natural role in immune system homeostasis, other therapeutic areas are also
involved, such as liver fibrosis, rheumatoid arthritis, Alzheimer’s disease, and
inflammatory diseases caused by bacterial infections. This review summarizes the
recent innovative developments in the design of nanodelivery systems modified with
TRAIL pathway-targeting ligands. © 2023 by the authors.
AU - Yagolovich, A. V.
AU - Gasparian, M. E.
AU - Dolgikh, D. A.
C7 - 515
DB - Scopus
IS - 2
KW - death receptors
DR5
drug delivery
ligand-targeted drugs
nanoparticles
nanotherapeutics
receptor clustering
TRAIL
antiinfective agent
caspase 8
CD47 antigen
death receptor
dendrimer
ferritin
folic acid
interleukin 4
nanomaterial
silver nanoparticle
tumor necrosis factor related apoptosis inducing ligand
amino acid sequence
apoptosis
binding affinity
biocompatibility
biodegradability
cancer inhibition
cell surface
cell survival
circulating tumor cell
colony formation
cross linking
DNA damage
drug synthesis
endocytosis
genetic engineering
human
human cell
Klebsiella pneumoniae infection
lactic acid bacterium
liposomal delivery
liver fibrosis
molecular dynamics
nanomedicine
Notch signaling
Review
signal transduction
thermal conductivity
tumor volume
upregulation
M3 - Review
PY - 2023
ST - Recent Advances in the Development of Nanodelivery Systems Targeting the
TRAIL Death Receptor Pathway
T2 - Pharmaceutics
TI - Recent Advances in the Development of Nanodelivery Systems Targeting the
TRAIL Death Receptor Pathway
85149115813&doi=10.3390%2fpharmaceutics15020515&partnerID=40&md5=307edb9b0ea82f941d
48e80455c41a16
VL - 15
ID - 4988
ER -
TY - JOUR
AB - In this research, a simple, green approach was employed to synthesize silver
nanoparticles with the aid of Ziziphus spina-christi (L.) methanol root extract,
which can act as a reducing, capping agent to treat obesity and inflammation.
Globally, Ziziphus spina-christi (Jujube) root is used in traditional therapy as a
lipolysis promoter. GC-MS results confirmed the availability of kaempferol
(flavonol), cannabinol and indole-3-carboxylic acid in Ziziphus spina-christi root
methanol extract (ZSE). ZSE silver nanoparticles (ZS-Ag-NPs) were synthesized and
their effect on mitochondrial fatty acid oxidation capacity and adipokine levels in
maturing adipocytes were analyzed. Maturing adipocytes treated with 0.4 mu g/dL of
ZSE and ZS-Ag-NPs significantly reduced the lipid content in adipocytes by 64% and
82%, respectively. In addition, lipolysis-related genes such as LPL (1.9 fold), HSL
(2.3 fold), PGC-1 alpha (3 fold), UCP-1 (4.1 fold), PRDM16 (2 fold) and PPAR alpha
(2.7 fold) increased significantly in ZS-Ag-NPs treated maturing adipocytes. The
ZS-Ag-NPs treatment significantly decreased insulin resistance and metabolic
inflammation-related LTB4-R, TNF-alpha, IL-4 and STAT-6 mRNA levels. Mitochondrial
thermogenesis stimulating capacity of ZS-Ag-NPs was further confirmed by the
significantly enhanced CREB-1 and AMPK protein levels in adipocytes. Furthermore,
ZS-Ag-NPs treated adipokines (condition media, CM) were treated with human
umbilical vein endothelial cells (HUVECs) to determine cytotoxicity and pro-
inflammatory stimulus capacity. We found that ZS-Ag-NPs treated adipocyte CM
effectively increased mRNA expression levels of the vascular endothelial cell
growth factor (VEGF), and down-regulated oxidative stress (LPO, eNOS, and HO) and
vascular cell inflammation (ICAM, VCAM, TNF-alpha, IL-1 beta, and NF-kappa B). In
conclusion, ZS-Ag-NPs displayed an action at the molecular level in mitochondrial
fatty acid oxidation, decreased adipokine secretion in adipocytes, and enhanced
vascular endothelial cell growth. This molecular mechanical action of ZS-Ag-NPs
reduced effectively obesity progressions and metabolic inflammatory pathogenesis
associated with aging.
AN - WOS:000712679900001
AU - Yagoub, A. A.
AU - Alshammari, G. M.
AU - Subash-Babu, P.
AU - Mohammed, M. A. A.
AU - Yahya, M. A.
AU - Alhosain, A. I.
C7 - 2563
DA - OCT
DO - 10.3390/nano11102563
IS - 10
PY - 2021
SN - 2079-4991
ST - Synthesis of Ziziphus spina-christi (Jujube) Root Methanol Extract Loaded
Functionalized Silver Nanoparticle (ZS-Ag-NPs); Physiochemical Characterization and
Effect of ZS-Ag-NPs on Adipocyte Maturation, Adipokine and Vascular Smooth Muscle
Cell Interaction
T2 - NANOMATERIALS
TI - Synthesis of Ziziphus spina-christi (Jujube) Root Methanol Extract Loaded
Functionalized Silver Nanoparticle (ZS-Ag-NPs); Physiochemical Characterization and
Effect of ZS-Ag-NPs on Adipocyte Maturation, Adipokine and Vascular Smooth Muscle
Cell Interaction
VL - 11
ID - 6291
ER -
TY - JOUR
AB - In this research, a simple, green approach was employed to synthesize silver
nanoparticles with the aid of Ziziphus spina-christi (L.) methanol root extract,
which can act as a reducing, capping agent to treat obesity and inflammation.
Globally, Ziziphus spina-christi (Jujube) root is used in traditional therapy as a
lipolysis promoter. GC-MS results confirmed the availability of kaempferol
(flavonol), cannabinol and indole-3-carboxylic acid in Ziziphus spina-christi root
methanol extract (ZSE). ZSE silver nanoparticles (ZS-Ag-NPs) were synthesized and
their effect on mitochondrial fatty acid oxidation capacity and adipokine levels in
maturing adipocytes were analyzed. Maturing adipocytes treated with 0.4 µg/dL of
ZSE and ZS-Ag-NPs significantly reduced the lipid content in adipocytes by 64% and
82%, respectively. In addition, lipolysis-related genes such as LPL (1.9 fold), HSL
(2.3 fold), PGC-1α (3 fold), UCP-1 (4.1 fold), PRDM16 (2 fold) and PPARα (2.7 fold)
increased significantly in ZS-Ag-NPs treated maturing adipocytes. The ZS-Ag-NPs
treatment significantly decreased insulin resistance and metabolic inflammation-
related LTB4-R, TNF-α, IL-4 and STAT-6 mRNA levels. Mitochondrial thermogenesis
stimulating capacity of ZS-Ag-NPs was further confirmed by the significantly
enhanced CREB-1 and AMPK protein levels in adipocytes. Furthermore, ZS-Ag-NPs
treated adipokines (condition media, CM) were treated with human umbilical vein
endothelial cells (HUVECs) to determine cytotoxicity and pro-inflammatory stimulus
capacity. We found that ZS-Ag-NPs treated adipocyte CM effectively increased mRNA
expression levels of the vascular endothelial cell growth factor (VEGF), and down-
regulated oxidative stress (LPO, eNOS, and HO) and vascular cell inflammation
(ICAM, VCAM, TNF-α, IL-1β, and NF-κB). In conclusion, ZS-Ag-NPs displayed an action
at the molecular level in mitochondrial fatty acid oxidation, decreased adipokine
secretion in adipocytes, and enhanced vascular endothelial cell growth. This
molecular mechanical action of ZS-Ag-NPs reduced effectively obesity progressions
and metabolic inflammatory pathogenesis associated with aging. © 2021 by the
AU - Yagoub, A. E. A.
AU - Alshammari, G. M.
AU - Subash-Babu, P.
AU - Mohammed, M. A. A.
AU - Yahya, M. A.
AU - Alhosain, A. I.
C7 - 2563
DB - Scopus
DO - 10.3390/nano11102563
IS - 10
KW - Adipocytes
Angiogenesis
Jujube
Silver nanoparticle
Ziziphus spina-christi (L.)
M3 - Article
PY - 2021
ST - Synthesis of ziziphus spina-christi (Jujube) root methanol extract loaded
functionalized silver nanoparticle (zs-ag-nps); physiochemical characterization and
effect of zs-ag-nps on adipocyte maturation, adipokine and vascular smooth muscle
cell interaction
T2 - Nanomaterials
TI - Synthesis of ziziphus spina-christi (Jujube) root methanol extract loaded
functionalized silver nanoparticle (zs-ag-nps); physiochemical characterization and
effect of zs-ag-nps on adipocyte maturation, adipokine and vascular smooth muscle
cell interaction
85115911352&doi=10.3390%2fnano11102563&partnerID=40&md5=f71592be9b37029a6d3b99eb307
266fb
VL - 11
ID - 5195
ER -
TY - JOUR
AB - A green synthesis of silver nanoparticles (AgNPs) using aqueous Lonicera
japonica leaf extract (AgNPs-LLJ) was reported and their anti-inflammatory and
antibacterial effects were investigated. In comparison, AgNPs were also synthesised
using the liquid phase chemical reduction method (AgNPs-N2H4H2O). The synthesised
AgNPs were identified using multiple analytical techniques. The results showed that
both AgNPs were spherical particles with an average particle size of 20-30 nm for
AgNPs-LLJ and 10-20 nm for AgNPs-N2H4H2O. The anti-inflammatory ability of AgNPs
was shown by the effective inhibition against 5-lipoxygenase with IC50 values of
5.08 μg/mL for AgNPs-LLJ and 59.12 μg/mL for AgNPs-N2H4H2O, indicating that AgNPs-
LLJ had much more obvious antiinflammatory effect. Furthermore, the cytotoxicity
research on RAW264.7 mouse macrophages showed that AgNPs-LLJ had no cytotoxicity at
the concentration of 40 μg/mL compared with control. Their antibacterial effects on
Escherichia coli (EC) and Staphlococcus aureus (SA) were studied by agar well
diffusion method. The maximum antibacterial circles of AgNPs-N2H4H2O on EC and SA
were 15 and 14 mm, respectively, whereas it was 5.0 and 5.0 mm for AgNPs-LLJ,
respectively. The authors conclude that AgNPs-LLJs have a potential to be a
nanomedicine for nanobiomedical applications. © The Institution of Engineering and
Technology 2019.
AU - Yan, L.
AU - Qiu, B.
AU - Li, T.
AU - Wu, D.
AU - Zhu, J.
AU - Zhao, D.
DB - Scopus
DO - 10.1049/mnl.2019.0343
IS - 2
KW - Escherichia coli
Metal nanoparticles
Particle size
gentamicin
nordihydroguaiaretic acid
silver nanoparticle
Anti-inflammatories
Average particle size
Chemical reduction methods
Diffusion method
Spherical particle
animal cell
Article
controlled study
drug cytotoxicity
drug synthesis
enzyme activity
enzyme inhibition
green chemistry
IC50
in vitro study
Lonicera japonica
macrophage
mouse
nanomedicine
nonhuman
particle size
plant leaf
RAW 264.7 cell line
zeta potential
Silver compounds
M3 - Article
PY - 2020
SP - 90-95
ST - Green synthesis of silver nanoparticles from Lonicera japonica leaf extract
and their anti-inflammatory and antibacterial effects
T2 - Micro and Nano Letters
TI - Green synthesis of silver nanoparticles from Lonicera japonica leaf extract
and their anti-inflammatory and antibacterial effects
85078860427&doi=10.1049%2fmnl.2019.0343&partnerID=40&md5=21539cadfc3374ad555b56ea15
0e4c19
VL - 15
ID - 5259
ER -
TY - JOUR
AB - Chronic sclerosing unspecific sialadenitis or KYttner tumor, is an infrequent
inflammatory lesion of submandibular gland. We report a 60 years old male
presenting with a slowly growing, painless, bilateral submandibular tumor of two
years of evolution. Pathological examinations showed marked atrophy of glandular
parenchyma with increased fibrous connective tissue and an intense lymphocytic
infiltration with lymphoid follicle formation. Lymphocyte population study with
kappa, lambda, CD20 and CD45RO antibodies was similar to that observed in reactive
lymph nodes. There was no over expression of Bcl-2 gene protein, involved in the
phenomenon of apoptosis of glandular tissue, that could explain the pathogenesis of
atrophy. This protein was positive only in lymphoid cells and glandular conducts.
An immune etiology, with replacement of glandular tissue by lymphoid and fibrous
connective tissue is suggested
AD - Yáñez M., Milly
Universidad de la Frontera. Facultad de Medicina. Hospital Regional Temuco. Unidad
de Anatomía Patológica Citopatología.
Roa Esterio, Iván
Roa S., Juan Carlos
Villaseca H., Miguel Angel
García V., Marcela
AU - Yáñez M, Milly
AU - Roa Esterio, Iván
AU - Roa S, Juan Carlos
AU - Villaseca H, Miguel Angel
AU - García V, Marcela
C1 - 19990714
DA - 1999/05
DB - LILACS
IS - 5
LA - es
PY - 1999
SN - 0034-9887
SP - 600-3
ST - Sialadenitis crónica esclerosante inespecífica de la glándula submandibular,
tumor de Kuttner: caso clínico
T2 - Rev. méd. Chile
TI - Sialadenitis crónica esclerosante inespecífica de la glándula submandibular,
tumor de Kuttner: caso clínico
TT - Chronic sclerosing unspecific sialadenitis of submandibular gland, Kuttner
tumor: report of case
UR - https://pesquisa.bvsalud.org/portal/resource/pt/lil-243935
VL - 127
ID - 4957
ER -
TY - JOUR
AB - Purpose: Pt-based nanostructures are one of the promising nanomaterials for
being used in catalysts, sensors, and therapeutics. However, their impacts on the
health and biological systems are not adequately understood yet.Methods: In this
work, nanorods composed of ultrasmall platinum (Pt) nanoparticles deposited on the
surface and gold nanorod as the core (Au@Pt NRs) were synthesized, and the
distribution and toxic effects of Au@Pt NRs were investigated in C57BL/6 mice with
intravenous injection by using atomic absorption spectroscopy (AAS), transmission
electron microscope (TEM), hematoxylin-eosin (HE) staining and blood cell
analyzer.Results: At the time point of Day 1, Day 8 and Day 16 post injection of
Au@Pt NRs (6 mg/kg of Pt atom), Au@Pt NRs were mainly accumulated in the liver and
spleen. The energy dispersive spectrometer mapping images showed Au@Pt NRs
experienced quick corrosion and Au released faster than Pt in the physiological
environments. The catalase (CAT) activity in tissues increased slightly in the
early stage of the Au@Pt NRs exposure and went down to the normal level. With HE
staining, inflammatory cells infiltration could be seen in the tissues, while no
significant influences were detected on the blood biochemistry and the function of
liver and kidney.Conclusion: In conclusion, intravenously injected Au@Pt NRs mainly
distributed in the liver and spleen with comparable levels, and did not exert any
significant toxic effects on the organs' function within two weeks; meanwhile,
Au@Pt NRs were able to degrade, which indicated acceptable safety to the mice and
potentials of biomedical application.
AN - WOS:000886268900001
AU - Yang, A. Y.
AU - Wen, T.
AU - Hao, B. Y.
AU - Meng, Y. L.
AU - Zhang, X.
AU - Wang, T.
AU - Meng, J.
AU - Liu, J.
AU - Wang, J. H.
AU - Xu, H. Y.
DO - 10.2147/IJN.S386476
PY - 2022
SN - 1178-2013
SP - 5339-5351
ST - Biodistribution and Toxicological Effects of Ultra-Small Pt Nanoparticles
Deposited on Au Nanorods (Au@Pt NRs) in Mice with Intravenous Injection
TI - Biodistribution and Toxicological Effects of Ultra-Small Pt Nanoparticles
Deposited on Au Nanorods (Au@Pt NRs) in Mice with Intravenous Injection
VL - 17
ID - 6592
ER -
TY - JOUR
AB - Polyphenols are micronutrients that are widely present in human daily diets.
Numerous studies have demonstrated their potential as antioxidants and anti-
inflammatory agents, and for cancer prevention, heart protection and the treatment
of neurodegenerative diseases. However, due to their vulnerability to environmental
conditions and low bioavailability, their application in the food and medical
fields is greatly limited. Nanoformulations, as excellent drug delivery systems,
can overcome these limitations and maximize the pharmacological effects of
polyphenols. In this review, we summarize the biological activities of polyphenols,
together with systems for their delivery, including phospholipid complexes, lipid-
based nanoparticles, protein-based nanoparticles, niosomes, polymers, micelles,
emulsions and metal nanoparticles. The application of polyphenol nanoparticles in
food and medicine is also discussed. Although loading into nanoparticles solves the
main limitation to application of polyphenolic compounds, there are some concerns
about their toxicological safety after entry into the human body. It is therefore
necessary to conduct toxicity studies and residue analysis on the carrier.
AN - WOS:000585679800001
AU - Yang, B. Y.
AU - Dong, Y. X.
AU - Wang, F.
AU - Zhang, Y.
C7 - 4613
DA - OCT
DO - 10.3390/molecules25204613
IS - 20
PY - 2020
SN - 1420-3049
ST - Nanoformulations to Enhance the Bioavailability and Physiological Functions
of Polyphenols
T2 - MOLECULES
TI - Nanoformulations to Enhance the Bioavailability and Physiological Functions
of Polyphenols
VL - 25
ID - 6685
ER -
TY - JOUR
AB - Bacterial infections pose a serious threat to human health, and the
development of new antibiotics has not kept pace with the development of bacterial
resistance. Therefore, there is an urgent need to design antibiotic-like nano-
formulations that break through bacterial resistance mechanisms. In this work, we
successfully synthesized a safe and effective antibacterial nano-formulation of
Se@Ag@EGCG by self-assembly of epigallocatechin gallate (EGCG)-coated silver
nanoparticles (Ag) on the surface of selenium nanowires (Se). The in vitro
bacteriostatic results showed that 40 mu g ml(-1) Se@Ag@EGCG had significant
antibacterial activity against drug-resistant Staphylococcus aureus (S. aureus) and
Escherichia coli (E. coli) by destroying the formation of bacterial biofilm,
promoting the production of high concentration reactive oxygen species and
destroying bacterial cell wall. In addition, the results of in vivo antibacterial
experiments showed that subcutaneous administration of 10 mg kg(-1) of Se@Ag@EGCG
could promote wound healing by reducing apoptosis and inflammatory responses in
infected wounds. It is worth mentioning that the reduced and modified Se@Ag@EGCG by
this natural product has negligible in vivo toxicity. This development strategy of
nano-antibacterial materials, which breaks through the drug resistance mechanism,
provides new ideas for the development of drugs for drug-resistant bacterial
infections.
AN - WOS:000827242400001
AU - Yang, C. H.
AU - Wang, Z. K.
AU - Gao, Y.
AU - Li, M.
AU - Li, Y. Q.
AU - Dai, C. X.
AU - Wang, Y. S.
AU - Sun, D. D.
C7 - 415101
DA - OCT 8
DO - 10.1088/1361-6528/ac7db0
IS - 41
PY - 2022
SN - 0957-4484
1361-6528
ST - EGCG-coated silver nanoparticles self-assemble with selenium nanowires for
treatment of drug-resistant bacterial infections by generating ROS and disrupting
biofilms
T2 - NANOTECHNOLOGY
TI - EGCG-coated silver nanoparticles self-assemble with selenium nanowires for
biofilms
VL - 33
ID - 6217
ER -
TY - JOUR
AB - Pure fish skin collagen hydrogels as a wound dressing have lower
thermodynamic stability than mammalian collagen and usually suffer from poor
mechanical properties, weak degradation resistance and insufficient functionalities
such as antioxidant and anti-inflammatory properties to meet clinical needs that
limit its further application. Here, a silver carp skin collagen hydrogel is
successfully constructed via the cross-linking of the laccase-protocatechuic
aldehyde (LAC-PAL) and the structure of the hydrogel is further consolidated and
strengthened by the interaction of PAL and Fe3+. In this collagen hydrogel system,
Fe3+, acting as a second cross -linker, consolidates and enhances the stability of
the hydrogel after LAC-PAL cross-linking. This cross-linking method improves the
resistance to degradation with a reduction in its degradation rate from 89.45% to
38.66% and endows the hydrogel with antioxidant activity. The in vitro data show
that the hydrogel promotes cell proliferation and adhesion exhibiting good
biocompatibility. Animal experiments show that the hydrogel contributes to
angiogenesis and improves inflammatory response in the early stages of wound
healing, resulting in promoting wound healing. Altogether, this newly developed
collagen hydrogel is expected to be applied in wound repair as a wound dressing.
AN - WOS:000862658700001
AU - Yang, C. K.
AU - Zhang, Y. T.
AU - Tang, P. P.
AU - Zheng, T. T.
AU - Zhang, X. X.
AU - Zhang, Y. Z.
AU - Li, G. Y.
C6 - SEP 2022
C7 - 112825
DA - NOV
DO - 10.1016/j.colsurfb.2022.112825
PY - 2022
SN - 0927-7765
1873-4367
ST - Collagen-based hydrogels cross-linked via laccase- mediated system
incorporated with Fe3+for wound dressing
TI - Collagen-based hydrogels cross-linked via laccase- mediated system
incorporated with Fe3+for wound dressing
VL - 219
ID - 6309
ER -
TY - JOUR
AB - Bacterial infections pose a serious threat to human health, and the
development of new antibiotics has not kept pace with the development of bacterial
resistance. Therefore, there is an urgent need to design antibiotic-like nano-
formulations that break through bacterial resistance mechanisms. In this work, we
successfully synthesized a safe and effective antibacterial nano-formulation of
Se@Ag@EGCG by self-assembly of epigallocatechin gallate (EGCG)-coated silver
nanoparticles (Ag) on the surface of selenium nanowires (Se). The in vitro
bacteriostatic results showed that 40 μg ml-1 Se@Ag@EGCG had significant
antibacterial activity against drug-resistant Staphylococcus aureus (S. aureus) and
Escherichia coli (E. coli) by destroying the formation of bacterial biofilm,
promoting the production of high concentration reactive oxygen species and
destroying bacterial cell wall. In addition, the results of in vivo antibacterial
experiments showed that subcutaneous administration of 10 mg kg-1 of Se@Ag@EGCG
could promote wound healing by reducing apoptosis and inflammatory responses in
infected wounds. It is worth mentioning that the reduced and modified Se@Ag@EGCG by
this natural product has negligible in vivo toxicity. This development strategy of
nano-antibacterial materials, which breaks through the drug resistance mechanism,
provides new ideas for the development of drugs for drug-resistant bacterial
infections. © 2022 IOP Publishing Ltd.
AU - Yang, C.
AU - Wang, Z.
AU - Gao, Y.
AU - Li, M.
AU - Li, Y.
AU - Dai, C.
AU - Wang, Y.
AU - Sun, D.
C7 - 415101
DB - Scopus
DO - 10.1088/1361-6528/ac7db0
IS - 41
KW - antibacterial
biofilm
selenium nanowires
self-assembly
Biofilms
Catechin
Escherichia coli
Humans
Metal Nanoparticles
Nanowires
Selenium
Silver
Antibiotics
Cell death
Cell membranes
Drug delivery
Health risks
Metal nanoparticles
Oxygen
antiinfective agent
catechin
metal nanoparticle
nanowire
selenium
silver
Antibacterials
Bacterial resistance
Human health
In-vivo
Resistance mechanisms
Selenia nanowire
Self-assemble
bacterial infection
human
Self assembly
M3 - Article
PY - 2022
ST - EGCG-coated silver nanoparticles self-assemble with selenium nanowires for
biofilms
T2 - Nanotechnology
TI - EGCG-coated silver nanoparticles self-assemble with selenium nanowires for
biofilms
85134720902&doi=10.1088%2f1361-
6528%2fac7db0&partnerID=40&md5=3ae59e2c4d22f06c6d9fc62f148fedbe
VL - 33
ID - 5081
ER -
TY - JOUR
AB - Pure fish skin collagen hydrogels as a wound dressing have lower
thermodynamic stability than mammalian collagen and usually suffer from poor
mechanical properties, weak degradation resistance and insufficient functionalities
such as antioxidant and anti-inflammatory properties to meet clinical needs that
limit its further application. Here, a silver carp skin collagen hydrogel is
successfully constructed via the cross-linking of the laccase-protocatechuic
aldehyde (LAC-PAL) and the structure of the hydrogel is further consolidated and
strengthened by the interaction of PAL and Fe3+. In this collagen hydrogel system,
Fe3+, acting as a second cross-linker, consolidates and enhances the stability of
the hydrogel after LAC-PAL cross-linking. This cross-linking method improves the
resistance to degradation with a reduction in its degradation rate from 89.45% to
38.66% and endows the hydrogel with antioxidant activity. The in vitro data show
that the hydrogel promotes cell proliferation and adhesion exhibiting good
biocompatibility. Animal experiments show that the hydrogel contributes to
angiogenesis and improves inflammatory response in the early stages of wound
healing, resulting in promoting wound healing. Altogether, this newly developed
collagen hydrogel is expected to be applied in wound repair as a wound dressing. ©
2022 Elsevier B.V.
AU - Yang, C.
AU - Zhang, Y.
AU - Tang, P.
AU - Zheng, T.
AU - Zhang, X.
AU - Li, G.
C7 - 112825
DB - Scopus
DO - 10.1016/j.colsurfb.2022.112825
KW - Antioxidant and anti-inflammatory
Collagen-based hydrogels
Wound dressing
Antioxidants
Biocompatibility
Biomechanics
Cell proliferation
Collagen
Degradation
Enzymes
Mammals
Anti-inflammatories
Collagen-based hydrogel
Cross linking
Fe 3+
Laccases
Protocatechuic aldehydes
Skin collagen
Wound dressings
Wound healing
Hydrogels
M3 - Article
PY - 2022
ST - Collagen-based hydrogels cross-linked via laccase - mediated system
incorporated with Fe3+ for wound dressing
TI - Collagen-based hydrogels cross-linked via laccase - mediated system
incorporated with Fe3+ for wound dressing
85137177733&doi=10.1016%2fj.colsurfb.2022.112825&partnerID=40&md5=7c1503c576621e3ff
d289f39519ae0d3
VL - 219
ID - 5051
ER -
TY - JOUR
AB - Multiple sclerosis (MS) is a chronic inflammatory disease of the central
nervous system (CNS) in which the immune system damages the protective insulation
surrounding nerve fibers that project from neurons. The pathological hallmark of MS
is multiple areas of myelin loss accompanied by inflammation within the CNS,
resulting in loss of cognitive function that ultimately leads to paralysis. Recent
studies in MS have focused on autophagy, a cellular self-eating process, as a
potential target for MS treatment. Here, we review the contribution of immune cell
autophagy to the pathogenesis of experimental autoimmune encephalomyelitis (EAE),
the prototypic animal model of MS. A better understanding of the role of autophagy
in different immune cells to EAE might inform the development of novel therapeutic
approaches in MS and other autoimmune and inflammatory diseases. © Copyright © 2021
Yang and Van Kaer.
AU - Yang, G.
AU - Van Kaer, L.
C7 - 724108
DB - Scopus
DO - 10.3389/fimmu.2021.724108
KW - autophagy
experimental autoimmune encephalomyelitis (EAE)
immune cells
LC3-associated phagocytosis (LAP)
multiple sclerosis
therapy
Adaptive Immunity
Animals
Autophagy
Encephalomyelitis, Autoimmune, Experimental
Humans
Immunity, Innate
Multiple Sclerosis
T-Lymphocytes
3 methyladenine
aspartic acid
autoantigen
autophagy related protein 5
autophagy related protein 7
caspase recruitment domain protein 15
chloroquine
estrogen receptor
fk 506 binding protein
interleukin 1beta
mammalian target of rapamycin
myelin
nicotinic receptor
pertussis toxin
procaspase 8
sequestosome 1
tamoxifen
apoptosis
axon
blood brain barrier
bone marrow cell
CD4+ T lymphocyte
cell infiltration
cognition
cytokine production
cytokinesis
cytoplasm
dendritic cell
endocytosis
experimental autoimmune encephalomyelitis
Golgi complex
immune system
immunization
intravital microscopy
lymphocyte
macrophage
melanogenesis
microglia
myelin sheath
nerve cell
nerve fiber
neutrophil
nonhuman
oligodendroglia
paralysis
pathogenesis
pathophysiology
peripheral lymphocyte
phagocytosis
phenotype
Review
single cell RNA seq
spinal cord
systematic review
T lymphocyte
Th1 cell
Th17 cell
thymocyte
adaptive immunity
animal
disease model
human
immunology
innate immunity
M3 - Review
PY - 2021
ST - Therapeutic Targeting of Immune Cell Autophagy in Multiple Sclerosis: Russian
Roulette or Silver Bullet?
TI - Therapeutic Targeting of Immune Cell Autophagy in Multiple Sclerosis: Russian
Roulette or Silver Bullet?
85114778006&doi=10.3389%2ffimmu.2021.724108&partnerID=40&md5=d193b97108faafee9c7ffd
b84a206aec
VL - 12
ID - 5229
ER -
TY - JOUR
AB - Titanium and Titanium alloys are widely used as biomedical implants in oral
and maxillofacial surgery, due to superior mechanical properties and
biocompatibility. In specific clinical populations such as the elderly, diabetics
and patients with metabolic diseases, the failure rate of medical metal implants is
increased significantly, putting them at increased risk of revision surgery. Many
studies show that the content of reactive oxygen species (ROS) in the
microenvironment of bone tissue surrounding implant materials is increased in
patients undergoing revision surgery. In addition, the size and shape of materials,
the morphology, wettability, mechanical properties, and other properties play
significant roles in the production of ROS. The accumulated ROS break the original
balance of oxidation and anti-oxidation, resulting in host oxidative stress. It may
accelerate implant degradation mainly by activating inflammatory cells. Peri-
implantitis usually leads to a loss of bone mass around the implant, which tends to
affect the long-term stability and longevity of implant. Therefore, a great deal of
research is urgently needed to focus on developing antibacterial technologies. The
addition of active elements to biomedical titanium and titanium alloys greatly
reduce the risk of postoperative infection in patients. Besides, innovative
technologies are developing new biomaterials surfaces conferring anti-infective
properties that rely on the production of ROS. It can be considered that ROS may
act as a messenger substance for the communication between the host and the
implanted material, which run through the entire wound repair process and play a
role that cannot be ignored. It is necessary to understand the interaction between
oxidative stress and materials, the effects of oxidative stress products on
osseointegration and implant life as well as ROS-induced bactericidal activity.
This helps to facilitate the development of a new generation of well-biocompatible
implant materials with ROS responsiveness, and ultimately prolong the lifespan of
implants.
AN - WOS:000906034600001
AU - Yang, J.
AU - Liu, C.
AU - Sun, H.
AU - Liu, Y.
AU - Liu, Z. G.
AU - Zhang, D.
AU - Zhao, G.
AU - Wang, Q.
AU - Yang, D. H.
C7 - 1092916
DA - DEC 19
DO - 10.3389/fbioe.2022.1092916
PY - 2022
SN - 2296-4185
ST - The progress in titanium alloys used as biomedical implants: From the view of
TI - The progress in titanium alloys used as biomedical implants: From the view of
VL - 10
ID - 6748
ER -
TY - JOUR
AB - Interactions between nanoparticles and viruses have attracted increasing
attention due to the antiviral activity of nanoparticles and the resulting
possibility to be employed as biomedical interventions. In this contribution, we
developed a very simple route to prepare uniform and stable silver nanoparticles
(AgNPs) with antiviral properties by using curcumin, which is a member of the
ginger family isolated from rhizomes of the perennial herb Curcuma longa and has a
wide range of biological activities like antioxidant, antifungal, antibacterial and
anti-inflammatory effects, and acts as reducing and capping agents in this
synthetic route. The tissue culture infectious dose (TCID50) assay showed that the
curcumin modified silver nanoparticles (cAgNPs) have a highly efficient inhibition
effect against respiratory syncytial virus (RSV) infection, giving a decrease of
viral titers about two orders of magnitude at the concentration of cAgNPs under
which no toxicity was found to the host cells. Mechanism investigations showed that
cAgNPs could prevent RSV from infecting the host cells by inactivating the virus
directly, indicating that cAgNPs are a novel promising efficient virucide for RSV.
© 2016 The Royal Society of Chemistry.
AU - Yang, X. X.
AU - Li, C. M.
AU - Huang, C. Z.
DB - Scopus
DO - 10.1039/c5nr07918g
IS - 5
KW - Antiviral Agents
Cell Line
Cell Survival
Curcumin
Drug Carriers
Dynamic Light Scattering
Humans
Interleukin-1beta
Interleukin-6
Metal Nanoparticles
Photoelectron Spectroscopy
Respiratory Syncytial Virus Infections
Respiratory Syncytial Viruses
RNA, Messenger
Silver
Virus Internalization
Antifungal agents
Antiviral agents
Metal nanoparticles
Nanoparticles
Tissue culture
Viruses
antivirus agent
curcumin
drug carrier
interleukin 1beta
interleukin 6
messenger RNA
metal nanoparticle
silver
Antiviral activities
Antiviral properties
Mechanism investigation
Respiratory syncytial virus
Respiratory Syncytial Virus infection
cell line
cell survival
chemistry
drug effects
genetics
human
Human respiratory syncytial virus
metabolism
virus entry
M3 - Article
PY - 2016
SP - 3040-3048
ST - Curcumin modified silver nanoparticles for highly efficient inhibition of
T2 - Nanoscale
TI - Curcumin modified silver nanoparticles for highly efficient inhibition of
84956862209&doi=10.1039%2fc5nr07918g&partnerID=40&md5=d80fae0a7ced71a804409e5e6b312
c15
VL - 8
ID - 5481
ER -
TY - JOUR
AB - A novel antibacterial strategy is urgently required to develop for solving
bacterial biofilm obstruction and bacterial drug resistance in the infected wound
healing process. Herein, the Chitosan/Bletilla striata polysaccharide composited
microneedles were prepared by chitosan, tannic acid, AgNO3 and Bletilla striata
polysaccharide through step centrifugation. In our design system, the porous
structure of microneedles gradually disappeared, and the mechanical properties were
significantly improved after multiple fillings. Ag+ is reduced in-situ to silver
nanoparticles by the abundant polyphenols of tannic acid, displaying antibacterial
effects both in vitro and vivo, even for methicillin resistant Staphylococcus
aureus. The addition of Bletilla striata polysaccharide increased the ability of
piercing biofilm and promoted wound healing. The microneedles exhibited good
biocompatibility and with function of piercing the bacterial biofilms, scavenging
excessive free radicals, inhibiting inflammatory factors, and promoting wound
healing. Therefore, the multifunctional composited microneedles show great
potential to promote infected and susceptible wound healing. © 2022
AU - Yang, X.
AU - Jia, M.
AU - Li, Z.
AU - Ma, Z.
AU - Lv, J.
AU - Jia, D.
AU - He, D.
AU - Zeng, R.
AU - Luo, G.
AU - Yu, Y.
DB - Scopus
DO - 10.1016/j.ijbiomac.2022.06.131
KW - Bacterial infection
Bletilla striata polysaccharide
Chitosan
In-situ synthesis
Microneedle
Wound healing
Metal Nanoparticles
Orchidaceae
Polysaccharides
Silver
Tannins
Wound Healing
Bacteria
Biofilms
Drug delivery
Flavonoids
Free radicals
Metal nanoparticles
Needles
Silver compounds
chitosan
dimeticone
interleukin 10
polyphenol
polysaccharide
silver nanoparticle
silver nitrate
tannin
trichloroethane
vasculotropin
antiinfective agent
metal nanoparticle
silver
tannin derivative
Antibacterials
Bacterial biofilm
Bletillum striatum polysaccharide
Composited
Microneedles
Striatum
Tannic acid
animal cell
animal experiment
animal model
apoptosis
Article
bacterial infection
biocompatibility
biofilm
Bletilla
cell proliferation
controlled study
cytotoxicity
Escherichia coli
in vitro study
MTT assay
nonhuman
oxidative stress
photoelectron spectroscopy
rat
tensile strength
wound healing
X ray diffraction
chemistry
M3 - Article
PY - 2022
SP - 550-559
ST - In-situ synthesis silver nanoparticles in chitosan/Bletilla striata
polysaccharide composited microneedles for infected and susceptible wound healing
TI - In-situ synthesis silver nanoparticles in chitosan/Bletilla striata
polysaccharide composited microneedles for infected and susceptible wound healing
85135500358&doi=10.1016%2fj.ijbiomac.2022.06.131&partnerID=40&md5=7f7f4857188b0158b
a4b32ea29663ea9
VL - 215
ID - 5075
ER -
TY - JOUR
AB - Infiltration of inflammatory cells, especially the M1 macrophages that
secrete various types of inflammation cytokines, play crucial roles in the
pathogenesis of rheumatoid arthritis (RA). To relief synovial inflammation, M1
macrophages must be eliminated or switched to anti-inflammatory M2 phenotype. We
herein developed folic acid modified silver nanoparticles (FA-AgNPs) that can
actively deliver into M1 macrophages to synergistically induce M1 macrophages
reduction and M2 macrophages polarization for effective RA treatment. The AgNPs was
facilely prepared, PEGylated and modified with FA to realize M1 macrophages
targeting delivery via folate receptor overexpressed on M1 macrophages surface.
After entering cells, FA-AgNPs dissolved and released Ag+ in response to
intracellular glutathione (GSH), which is the key element to exert a series of
anti-inflammatory functions, such as M1 macrophages apoptosis and reactive oxygen
species (ROS) scavenging to facilitate M2 macrophages polarization, both of which
contributed to RA treatment. This nano-system could passively accumulate into
inflamed joints, permit potent anti-inflammatory activity, and impose strong
therapeutic efficacy in mice RA models with high biosafety. After treatment, FA-
AgNPs could be gradually cleared from the body mainly via feces without tissue
accumulation, and did not show any appreciable long-term toxicity. This work
declares the first example of using bio-active nanoparticles for RA treatment
without loading any drugs, and highlights the potential of FA-AgNPs for targeted RA
therapy via simultaneous M1 macrophage apoptosis and M1-to-M2 macrophages re-
polarization.
AN - WOS:000587813600010
AU - Yang, Y. H.
AU - Guo, L. N.
AU - Wang, Z.
AU - Liu, P.
AU - Liu, X. J.
AU - Ding, J. S.
AU - Zhou, W. H.
C7 - 120390
DA - JAN
PY - 2021
SN - 0142-9612
1878-5905
ST - Targeted silver nanoparticles for rheumatoid arthritis therapy via macrophage
apoptosis and Re-polarization
T2 - BIOMATERIALS
TI - Targeted silver nanoparticles for rheumatoid arthritis therapy via macrophage
VL - 264
ID - 5870
ER -
TY - JOUR
AB - Infiltration of inflammatory cells, especially the M1 macrophages that
secrete various types of inflammation cytokines, play crucial roles in the
pathogenesis of rheumatoid arthritis (RA). To relief synovial inﬂammation, M1
macrophages must be eliminated or switched to anti-inﬂammatory M2 phenotype. We
herein developed folic acid modified silver nanoparticles (FA-AgNPs) that can
actively deliver into M1 macrophages to synergistically induce M1 macrophages
reduction and M2 macrophages polarization for effective RA treatment. The AgNPs was
facilely prepared, PEGylated and modified with FA to realize M1 macrophages
targeting delivery via folate receptor overexpressed on M1 macrophages surface.
After entering cells, FA-AgNPs dissolved and released Ag+ in response to
intracellular glutathione (GSH), which is the key element to exert a series of
anti-inflammatory functions, such as M1 macrophages apoptosis and reactive oxygen
species (ROS) scavenging to facilitate M2 macrophages polarization, both of which
contributed to RA treatment. This nano-system could passively accumulate into
inflamed joints, permit potent anti-inflammatory activity, and impose strong
therapeutic efficacy in mice RA models with high biosafety. After treatment, FA-
AgNPs could be gradually cleared from the body mainly via feces without tissue
accumulation, and did not show any appreciable long-term toxicity. This work
declares the first example of using bio-active nanoparticles for RA treatment
without loading any drugs, and highlights the potential of FA-AgNPs for targeted RA
therapy via simultaneous M1 macrophage apoptosis and M1-to-M2 macrophages re-
polarization. © 2020 Elsevier Ltd
AU - Yang, Y.
AU - Guo, L.
AU - Wang, Z.
AU - Liu, P.
AU - Liu, X.
AU - Ding, J.
AU - Zhou, W.
C7 - 120390
DB - Scopus
KW - Macrophage polarization
Nanomedicine
ROS scavenge
Targeted therapy
Animals
Apoptosis
Arthritis, Rheumatoid
Macrophages
Metal Nanoparticles
Mice
Silver
Cell death
Diseases
Dissolved oxygen
Mammals
Metal nanoparticles
Nanosystems
Polarization
folate receptor
folic acid
glutathione
silver nanoparticle
metal nanoparticle
silver
Anti-inflammatory function
Folate receptor
Inflammatory cells
Targeting deliveries
Therapeutic efficacy
Tissue accumulation
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
bioaccumulation
cell polarity
cell surface
controlled study
drug accumulation
drug efficacy
drug safety
drug synthesis
macrophage
male
mouse
nanomedicine
nonhuman
PEGylation
polarization
priority journal
protein expression
animal
M3 - Article
PY - 2021
ST - Targeted silver nanoparticles for rheumatoid arthritis therapy via macrophage
T2 - Biomaterials
TI - Targeted silver nanoparticles for rheumatoid arthritis therapy via macrophage
85091503476&doi=10.1016%2fj.biomaterials.2020.120390&partnerID=40&md5=8e35c717a3d29
9949ee497d8313bb229
VL - 264
ID - 5281
ER -
TY - JOUR
AB - The present study was to investigate the effects of visfatin on the
morphological structure and function of the rat uterus during inflammation. The
expression and distribution of visfatin, morphological structure, eosinophils
(EOS), myeloperoxidase (MPO) and cytokines in the uterus of the LPS-induced rat
were studied using hematoxylin-eosin staining (HE), immunohistochemical methods,
western blots and enzyme-linked immunosorbent assay (ELISA). The present study
showed that visfatin positive cells dispersed widely in the uterus, and strong
positive staining was observed mainly in the cell cytoplasm. Compared with saline
group, in visfatin group, more uterine glands were found, EOS increased, and the
difference was significant (P<0.05), MPO reduced, and the difference was
significant (P<0.01). In addition, visfatin was able to increase the secretion of
IL-1b, IL-6, and TNF-a (P<0.01). Compared with LPS group, in vifatin+LPS group, the
uterine glands of the lamina propria increased, the myometrium became thinner, the
number of EOS and MPO reduced obviously, but the difference was not significant
(P>0.05), and after LPS stimulated body, visfatin decrease the level of IL-1b, IL-
6, TNF-a (P<0.01). The above results suggest that visfatin could affect the
morphological structure of rat uterus; Visfatin could modulate the inflammatory
response in rats' uterus by regulating the quantity of inflammatory cells, such as
EOS and MPO, and the level of inflammatory cytokines, such as IL-1b, IL-6, TNF-a.
El objetivo del presente estudio fue investigar los efectos de la visfatina sobre
la estructura morfológica y la función del útero de la rata durante la inflamación.
Se estudiaron la expresión y distribución de la visfatina, la estructura
morfológica, eosinófilos, mieloperoxidasa y citoquinas en el útero de rata mediante
la tinción de H&E, métodos inmunohistoquímicos, Western blots y ELISA. El estudio
mostró que las células visfatina positivas se dispersan ampliamente en el útero,
junto a una fuerte tinción positiva, principalmente en el citoplasma de la célula.
En comparación con el grupo control, en el grupo visfatina, se encontraron más
glándulas uterinas, se observó un aumento de EOS y la diferencia fue significativa
(p<0,05), MPO reducida siendo esta diferencia también significativa (p<0,01).
Además, la visfatina fue capaz de aumentar la secreción de IL-1b, IL-6 y TNF-a
(P<0,01). En comparación con el grupo LPS, visfatina+grupo LPS, las glándulas
uterinas de la lámina propia aumentaron, se observó un miometrio más delgado, y
número reducido de EOS y MPO, sin embargo, la diferencia no fue significativa
(P>0,05). Después de estímulo LPS en el cuerpo, se registró un nivel menor de
visfatina en IL-1b, IL-6, TNF-a (P<0,01). Los resultados anteriores sugieren que
visfatina podría afectar a la estructura morfológica del útero de rata. Además,
podría modular la respuesta inflamatoria en el útero mediante la regulación de la
cantidad de células inflamatorias, tales como EOS y MPO.
AD - Yang, Zhi
Huazhong Agricultural University. College of Veterinary Medicine. Wuhan. CN
Xiao, Ke
Wang, Wei
Tang, Juan
Sun, Peng-Peng
Peng, Ke-Mei
Song, Hui
AU - Yang, Zhi
AU - Xiao, Ke
AU - Wang, Wei
AU - Tang, Juan
AU - Sun, Peng-Peng
AU - Peng, Ke-Mei
AU - Song, Hui
C1 - 20150430
DA - 2015/03
DB - LILACS
DO - 10.4067/S0717-95022015000100031
IS - 1
KW - Citoquinas
Cytokine
Inflamación
Inflammation
LPS-induced rat
Rata inducida con lipopolisacáricos
Utero
Uterus
Visfatin
Visfatina
LA - en
PY - 2015
SN - 0717-9367
SP - 194-203
ST - The effect of visfatin on inflammatory reaction in uterus of LPS-induced rats
TI - The effect of visfatin on inflammatory reaction in uterus of LPS-induced rats
TT - Efecto de visfatina en la reacción inflamatoria en el útero de ratas
inducidas por LPS
95022015000100031
VL - 33
ID - 4936
ER -
TY - JOUR
AB - Titanium (Ti) with nanoscale structure on the surface exhibits excellent
biocompatibility and bone integration. Once implanted, the surgical implantation
may lead to bacterial infection and inflammatory reaction, which cause the implant
failure. In this work, irregular and nanorod-shaped ZnO nanoparticles were doped
into TiO2 nanotubes (TNTs) with inner diameter of about 50 nm by electro-
deposition. The antibacterial properties of ZnO incorporated into TiO2 nanotubes
(TNTs/ZnO) were evaluated using Staphylococcus aureus (S. aureus). Zn ions released
from the nanoparticles and the morphology could work together, improving
antibacterial effectiveness up to 99.3% compared with the TNTs. Macrophages were
cultured on the samples to determine their respective anti-inflammatory properties.
The proliferation and viability of macrophages were evaluated by the CCK-8 method
and Live&Dead stain, and the morphology of the cells was observed by scanning
electron microscopy. Results indicated that TNTs/ZnO has a significant inhibitory
effect on the proliferation and adhesion of macrophages, which could be used to
prevent chronic inflammation and control the inflammatory reaction. Besides, the
release of Zn ions from the ZnO nanoparticles is a long-term process, which could
be beneficial for bone integration. Results demonstrate that ZnO deposited into
TNTs improved the antibacterial effectiveness and weakened the inflammatory
reaction of titanium-based implants, which is a promising approach to enhance their
bioactivity.
AN - WOS:000430327900001
AU - Yao, S. L.
AU - Feng, X. J.
AU - Lu, J. J.
AU - Zheng, Y. D.
AU - Wang, X. M.
AU - Volinsky, A. A.
AU - Wang, L. N.
C7 - 244003
DA - JUN 15
DO - 10.1088/1361-6528/aabac1
IS - 24
PY - 2018
SN - 0957-4484
1361-6528
ST - Antibacterial activity and inflammation inhibition of ZnO nanoparticles
embedded TiO2 nanotubes
T2 - NANOTECHNOLOGY
TI - Antibacterial activity and inflammation inhibition of ZnO nanoparticles
embedded TiO2 nanotubes
VL - 29
ID - 6605
ER -
TY - JOUR
AB - Metallic nanoparticles (MNPs) are new engineering materials with broad
prospects for biomedical applications; thus, their biosafety has drawn great
concern. The liver is the main detoxification organ of vertebrates. However, many
issues concerning the interactions between MNPs and biological systems (cells and
tissues) are unclear, particularly the toxic effects of MNPs on hepatocytes and
other liver cells. Numerous researchers have shown that some MNPs can induce
decreased cell survival rate, production of reactive oxygen species (ROS),
mitochondrial damage, DNA strand breaks, and even autophagy, pyroptosis, apoptosis,
or other forms of cell death. Our review focuses on the recent researches on the
liver toxicity of MNPs and its mechanisms at cellular and subcellular levels to
provide a scientific basis for the subsequent hepatotoxicity studies of MNPs.
AN - WOS:000494691900003
AU - Yao, Y.
AU - Zang, Y. T.
AU - Qu, J.
AU - Tang, M.
AU - Zhang, T.
DO - 10.2147/IJN.S212907
PY - 2019
SN - 1178-2013
SP - 8787-8804
ST - The Toxicity Of Metallic Nanoparticles On Liver: The Subcellular Damages,
Mechanisms, And Outcomes
TI - The Toxicity Of Metallic Nanoparticles On Liver: The Subcellular Damages,
Mechanisms, And Outcomes
VL - 14
ID - 6322
ER -
TY - JOUR
AB - Rationale: Endophthalmitis, which is one of the severest complications of
cataract surgeries, can seriously threaten vision and even lead to irreversible
blindness owing to its complicated microenvironment, including both local bacterial
infection and severe inflammation. It is urgent to develop a comprehensive
treatment for both anti-bacterial and anti-inflammatory effects. Methods: Herein,
we developed AuAgCu2O-bromfenac sodium nanoparticles (AuAgCu2O-BS NPs), which was
designed to combine anti-bacterial and anti-inflammatory effects for integrated
therapy of endophthalmitis after cataract surgery. The AuAgCu2O-BS NPs could
eradicate methicillin-resistant Staphylococcus aureus (MRSA) bacterial strain
relied on their photodynamic effects and the release of metal ions (Ag+ and Cu+) by
the hollow AuAgCu2O nanostructures mediated mild photothermal effects. The anti-
inflammatory drug, bromfenac sodium, released from the nanoparticles were able to
significantly reduce the local inflammation of the endophthalmitis and promote
tissue rehabilitation. In vivo bacterial elimination and anti-inflammation were
confirmed by a postcataract endophthalmitis rabbit model. Results: Excellent
antibacterial ability of AuAgCu2O-BS NPs was verified both in vitro and in vivo.
Ophthalmological clinical observation and pathologic histology analysis showed
prominent treatment of inflammatory reaction. Importantly, the mild temperature
photothermal effect not only promoted the release of metal ions and bromfenac
sodium but also avoided the thermal damage of the surrounding tissues, which was
more suitable for the practical application of ophthalmology due to the complex
structure of the eyeball. Moreover, superior biocompatibility was approved by the
preliminary toxicity investigations, including low cytotoxicity, negligible damage
to major organs, and stable intraocular pressure. Conclusions: Our studies of
nanosystem provide a promising synergic therapeutic strategy for postcataract
endophthalmitis treatment with favorable prognosis and promise in clinical
translations.
AN - WOS:000548563800006
AU - Ye, Y.
AU - He, J.
AU - Qiao, Y.
AU - Qi, Y. C.
AU - Zhang, H. B.
AU - Santos, H. A.
AU - Zhong, D. N.
AU - Li, W. L.
AU - Hua, S. Y.
AU - Wang, W.
AU - Grzybowski, A.
AU - Yao, K.
AU - Zhou, M.
DO - 10.7150/thno.46895
IS - 19
PY - 2020
SN - 1838-7640
SP - 8541-8557
ST - Mild temperature photothermal assisted anti-bacterial and anti-inflammatory
nanosystem for synergistic treatment of post-cataract surgery endophthalmitis
T2 - THERANOSTICS
TI - Mild temperature photothermal assisted anti-bacterial and anti-inflammatory
VL - 10
ID - 6151
ER -
TY - JOUR
AB - Rationale: Endophthalmitis, which is one of the severest complications of
cataract surgeries, can seriously threaten vision and even lead to irreversible
blindness owing to its complicated microenvironment, including both local bacterial
infection and severe inflammation. It is urgent to develop a comprehensive
treatment for both anti-bacterial and anti-inflammatory effects. Methods: Herein,
we developed AuAgCu2O-bromfenac sodium nanoparticles (AuAgCu2O-BS NPs), which was
designed to combine anti-bacterial and anti-inflammatory effects for integrated
therapy of endophthalmitis after cataract surgery. The AuAgCu2O-BS NPs could
eradicate methicillin-resistant Staphylococcus aureus (MRSA) bacterial strain
relied on their photodynamic effects and the release of metal ions (Ag+ and Cu+) by
the hollow AuAgCu2O nanostructures mediated mild photothermal effects. The anti-
inflammatory drug, bromfenac sodium, released from the nanoparticles were able to
significantly reduce the local inflammation of the endophthalmitis and promote
tissue rehabilitation. In vivo bacterial elimination and anti-inflammation were
confirmed by a postcataract endophthalmitis rabbit model. Results: Excellent
antibacterial ability of AuAgCu2O-BS NPs was verified both in vitro and in vivo.
Ophthalmological clinical observation and pathologic histology analysis showed
prominent treatment of inflammatory reaction. Importantly, the mild temperature
photothermal effect not only promoted the release of metal ions and bromfenac
sodium but also avoided the thermal damage of the surrounding tissues, which was
more suitable for the practical application of ophthalmology due to the complex
structure of the eyeball. Moreover, superior biocompatibility was approved by the
preliminary toxicity investigations, including low cytotoxicity, negligible damage
to major organs, and stable intraocular pressure. Conclusions: Our studies of
nanosystem provide a promising synergic therapeutic strategy for postcataract
endophthalmitis treatment with favorable prognosis and promise in clinical
translations. © The author(s). This is an open access article distributed under the
terms of the Creative Commons Attribution License
(https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for
full terms and conditions.
AU - Ye, Y.
AU - He, J.
AU - Qiao, Y.
AU - Qi, Y.
AU - Zhang, H.
AU - Santos, H. A.
AU - Zhong, D.
AU - Li, W.
AU - Hua, S.
AU - Wang, W.
AU - Grzybowski, A.
AU - Yao, K.
AU - Zhou, M.
DB - Scopus
DO - 10.7150/thno.46895
IS - 19
KW - Antibacterial and anti-inflammation effect
Endophthalmitis
Mild photothermal therapy
Nanoparticles
Post-cataract Surgery
Animals
Benzophenones
Bromobenzenes
Cataract Extraction
Copper
Drug Synergism
Drug Therapy
Gold
Humans
Metal Nanoparticles
Microbial Viability
Photothermal Therapy
Rabbits
Silver
Treatment Outcome
bromfenac
copper
gold
nanoparticle
oxygen
silver
antiinfective agent
benzophenone derivative
bromobenzene
cuprous oxide
metal nanoparticle
animal experiment
animal model
animal tissue
Article
bacterial clearance
bacterial strain
biocompatibility
cataract extraction
controlled study
cytotoxicity
endophthalmitis
eyeball
female
histology
human
human cell
in vitro study
in vivo study
intraocular pressure
Leporidae
nonhuman
photodynamics
prognosis
thermal injury
thermoregulation
adverse event
animal
chemistry
disease model
drug effect
drug potentiation
drug therapy
microbial viability
microbiology
treatment outcome
M3 - Article
PY - 2020
SP - 8541-8557
ST - Mild temperature photothermal assisted anti-bacterial and anti-inflammatory
T2 - Theranostics
TI - Mild temperature photothermal assisted anti-bacterial and anti-inflammatory
85089131344&doi=10.7150%2fthno.46895&partnerID=40&md5=03537e034b8ba8e33b1c1a635f574
8a9
VL - 10
ID - 5422
ER -
TY - JOUR
AB - Chlamydia trachomatis is a very common sexually transmissible infection in
both developing and developed countries. A hallmark of C. trachomatis infection is
the induction of severe inflammatory responses which play critical roles in its
pathogenesis. Antibiotics are the only treatment option currently available for
controlling C. trachomatis infection; however, they are efficacious only when
administered early after an infection. The objectives of this study are to explore
alternative strategies in the control and regulation of inflammatory responses
triggered by a C. trachomatis infection. We employed silver-polyvinyl pyrrolidone
(Ag-PVP) nanoparticles, which have been shown to possess anti-inflammatory
properties, as our target and the in vitro mouse J774 macrophage model of C.
trachomatis infection. Our hypothesis is that small sizes of Ag-PVP nanoparticles
will control inflammatory mediators triggered by a C. trachomatis infection.
Cytotoxicity studies using Ag-PVP nanoparticles of 10, 20, and 80 nm sizes
revealed. 80% macrophage viability up to a concentration of 6.25 μg/mL, with the
10nm size being the least toxic. All sizes of Ag-PVP nanoparticles, especially the
10 nm size, reduced the levels of the prototypic cytokines, tumor necrosis factor
(TNF) and interleukin (IL)-6, as elicited from C. trachomatis infected macrophages.
Additionally, Ag-PVP nanoparticles (10 nm) selectively inhibited a broad spectrum
of other cytokines and chemokines produced by infected macrophages. Of
significance, Ag-PVP nanoparticles (10nm) caused perturbations in a variety of
upstream (toll like receptor 2 [TLR2], nucleotide-binding oligomerization-protein 2
[NOD2], cluster of differentiation [CD]40, CD80, and CD86) and downstream (IL-1
receptor-associated kinase 3 [IRAK3] and matrix metallopeptidase 9 [MMP9])
inflammatory signaling pathways by downregulating their messenger ribonucleic acid
(mRNA) gene transcript expressions as induced by C. trachomatis in macrophages.
Collectively, our data provides further evidence for the anti-inflammatory
properties of Ag-PVP nanoparticles, and opens new possibilities for smaller sizes
of Ag-PVP nanoparticles to be employed as regulators of inflammatory responses
induced by C. trachomatis. © 2013 Yilma et al, publisher and licensee Dove Medical
Press Ltd.
AU - Yilma, A. N.
AU - Singh, S. R.
AU - Dixit, S.
AU - Dennis, V. A.
DB - Scopus
KW - Bacteria
Chemokines
Cytokines
NOD2
Sexually transmitted disease
TLR2
Animals
B7 Antigens
Cell Line
Host-Pathogen Interactions
Macrophages
Metal Nanoparticles
Mice
Povidone
Silver
bacteria
chemokines
cytokines
sexually transmitted disease
B7 antigen
caspase recruitment domain protein 15
CD40 antigen
CD86 antigen
chemokine
cytokine
gelatinase B
interleukin 1 receptor associated kinase 3
interleukin 6
messenger RNA
povidone
silver nanoparticle
animal cell
animal experiment
animal model
article
cell viability
chlamydiasis
cytokine production
downstream processing
drug cytotoxicity
gene expression
in vitro study
lymphocyte differentiation
macrophage
mouse
nonhuman
particle size
signal transduction
M3 - Article
PY - 2013
SP - 2421-2432
ST - Anti-inflammatory effects of silver-polyvinyl pyrrolidone (Ag-PVP)
nanoparticles in mouse macrophages infected with live Chlamydia trachomatis
TI - Anti-inflammatory effects of silver-polyvinyl pyrrolidone (Ag-PVP)
nanoparticles in mouse macrophages infected with live Chlamydia trachomatis
84880092437&partnerID=40&md5=5b4107ca7ffaedae6a7732820672742f
VL - 8
ID - 5677
ER -
TY - CONF
AB - Chlamydia trachomatis infects macrophages and epithelial cells, which evoke
TNF, IL-6, and IL-8 that are key protagonists of acute inflammation. These
cytokines put the patient at risk for major health issues such as pelvic
inflammation disease (PID) and infertility, if not controlled. Surface coated pure
metal such as silver-polyvinyl pyrrolidone nanoparticle (Ag-PVP) has been studied
extensively for its anti-inflammatory role. Here we explored the hypothesis that
Ag-PVP will inhibit inflammatory cytokines that are produced during the early phase
of a C. trachomatis infection. We used human epithelial cells and mouse J774
macrophages as target cells, and live C. trachomatis serovar L2 and its major outer
membrane protein (MOMP) as stimulants. Ag-PVP added to 2 day C. trachomatis-
infected cells down-regulated TNF, IL-6 and IL-8. When MOMP was used as the
stimulant, Ag-PVP similarly down-regulated these cytokines. Results from the MTT
cytotoxicity assay clearly show that the anti-inflammatory effect of Ag-PVP was not
due to cell death. Our data imply that Ag-PVP maybe an important therapeutic agent
to regulate inflammation during the early stage of a C. trachomatis infection.
AU - Yilma, A. N.
AU - Singh, S. R.
AU - Taha, M. A.
AU - Fairley, S. J.
AU - Dennis, V. A.
DB - Scopus
KW - Bacteria
Cytokines
Inflammation
MOMP
Biological membranes
Cell death
Fluidics
Health risks
Macrophages
Nanoparticles
Nanotechnology
Pathology
Anti-inflammatories
Cytotoxicity assays
Epithelial cells
Health issues
HeLa cell
Human epithelial cells
Major outer membrane proteins
Pure metals
Serovar
Target cells
Therapeutic agents
Silver
PY - 2011
SP - 447-450
ST - Evidence of the anti-inflammatory effect of silver-polyvinyl pyrrolidone
nanoparticles (Ag-PVP) in Chlamydia trachomatis infected macrophages and HeLa cells
T2 - Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo,
NSTI-Nanotech 2011
TI - Evidence of the anti-inflammatory effect of silver-polyvinyl pyrrolidone
nanoparticles (Ag-PVP) in Chlamydia trachomatis infected macrophages and HeLa cells
81455139824&partnerID=40&md5=3c7d08a817f2c51c9a36a2ec66dfe083
VL - 3
ID - 5696
ER -
TY - CPAPER
AB - Chlamydia trachomatis infects macrophages and epithelial cells, which evoke
TNF, IL-6, and IL-8 that are key protagonists of acute inflammation. These
cytokines put the patient at risk for major health issues such as pelvic
inflammation disease (PID) and infertility, if not controlled. Surface coated pure
metal such as silver-polyvinyl pyrrolidone nanoparticle (Ag-PVP) has been studied
extensively for its anti-inflammatory role. Here we explored the hypothesis that
Ag-PVP will inhibit inflammatory cytokines that are produced during the early phase
of a C. trachomatis infection. We used human epithelial cells and mouse J774
macrophages as target cells, and live C. trachomatis serovar L2 and its major outer
membrane protein (MOMP) as stimulants. AgPVP added to 2 day C. trachomatis-infected
cells down-regulated TNF, IL-6 and IL-8. When MOMP was used as the stimulant, Ag-
PVP similarly down regulated these cytokines. Results from the MTT cytotoxicity
assay clearly show that the antiinflammatory effect of Ag-PVP was not due to cell
death. Our data imply that Ag-PVP maybe an important therapeutic agent to regulate
inflammation during the early stage of a C. trachomatis infection.
AN - WOS:000394061000118
AU - Yilma, A. N.
AU - Singh, S. R.
AU - Taha, M. A.
AU - Fairley, S. J.
AU - Dennis, V. A.
PY - 2011
SP - 447-450
T2 - NANOTECHNOLOGY 2011: BIO SENSORS, INSTRUMENTS, MEDICAL, ENVIRONMENT AND
ENERGY, NSTI-NANOTECH 2011, VOL 3
TI - Evidence of the Anti-inflammatory Effect of Silver-Polyvinyl Pyrrolidone
Nanoparticles (Ag-PVP) in Chlamydia trachomatis Infected Macrophages and HeLa Cells
ID - 5939
ER -
TY - JOUR
AB - Bacterial elimination is the key to the treatment of periapical
periodontitis. However, existing root canal disinfectants have not fully met this
requirement. Here, a new disinfectant candidate, nanosilver, is provided and its
efficacy against root canal infection is analyzed. Compared to calcium hydroxide,
the nanosilver, which is evenly loaded into the poly(lactic-co-glycolic acid)
carrier, shows excellent bactericidal activity against free-living Enterococcus
faecalis, stable-phase E. faecalis biofilm, and starved-phase E. faecalis biofilm,
which is difficult to eliminate through root canal therapy at present. Nanosilver
also exhibits great biocompatibility as it does not affect the proliferation of
primary pulp cells. In lipopolysaccharide-triggered periapical periodontitis, the
application of nanosilver significantly inhibits the expression of several
inflammation-related factors, including TLR4, Caspase-1, and Caspase-11.
Furthermore, nanosilver also has a great anti-inflammatory effect on inhibiting the
progression of canine chronic periapical periodontitis. The quantity of E. faecalis
in canals and the shadow area around the apex significantly decrease after
application of 1% and 2% nanosilver for 4 weeks. It is demonstrated that 1% and 2%
nanosilver has a superior ability to resist periapical periodontitis in vivo and in
vitro.
AN - WOS:000496470400011
AU - Yin, W.
AU - Zheng, Z.
AU - Liu, Y. L.
AU - Wang, L. N.
AU - Shi, C.
AU - Zhang, L. L.
AU - Liu, S.
AU - Niu, W. D.
AU - Ting, K.
AU - Bian, Z.
C7 - 1900378
DA - NOV
DO - 10.1002/smtd.201900378
IS - 11
PY - 2019
SN - 2366-9608
ST - Disinfection of Infected Root Canals: Nanosilver Has Good Potential
T2 - SMALL METHODS
TI - Disinfection of Infected Root Canals: Nanosilver Has Good Potential
VL - 3
ID - 6652
ER -
TY - JOUR
AB - Bacterial elimination is the key to the treatment of periapical
periodontitis. However, existing root canal disinfectants have not fully met this
requirement. Here, a new disinfectant candidate, nanosilver, is provided and its
efficacy against root canal infection is analyzed. Compared to calcium hydroxide,
the nanosilver, which is evenly loaded into the poly(lactic-co-glycolic acid)
carrier, shows excellent bactericidal activity against free-living Enterococcus
faecalis, stable-phase E. faecalis biofilm, and starved-phase E. faecalis biofilm,
which is difficult to eliminate through root canal therapy at present. Nanosilver
also exhibits great biocompatibility as it does not affect the proliferation of
primary pulp cells. In lipopolysaccharide-triggered periapical periodontitis, the
application of nanosilver significantly inhibits the expression of several
inflammation-related factors, including TLR4, Caspase-1, and Caspase-11.
Furthermore, nanosilver also has a great anti-inflammatory effect on inhibiting the
progression of canine chronic periapical periodontitis. The quantity of E. faecalis
in canals and the shadow area around the apex significantly decrease after
application of 1% and 2% nanosilver for 4 weeks. It is demonstrated that 1% and 2%
nanosilver has a superior ability to resist periapical periodontitis in vivo and in
vitro. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
AU - Yin, W.
AU - Zheng, Z.
AU - Liu, Y.
AU - Wang, L.
AU - Shi, C.
AU - Zhang, L.
AU - Liu, S.
AU - Niu, W.
AU - Ting, K.
AU - Bian, Z.
C7 - 1900378
DB - Scopus
DO - 10.1002/smtd.201900378
IS - 11
KW - absorption
nanosilver
periapical periodontitis
poly(lactic-co-glycolic acid) (PLGA)
Absorption
Bacilli
Biocompatibility
Biofilms
Diseases
Disinfectants
Hydrated lime
Hydraulic structures
Lime
Bactericidal activity
Lipopolysaccharides
Nano silver
Poly(lactic-co-glycolic acid)
Root Canal Therapy
Canals
M3 - Article
PY - 2019
ST - Disinfection of Infected Root Canals: Nanosilver Has Good Potential
T2 - Small Methods
TI - Disinfection of Infected Root Canals: Nanosilver Has Good Potential
85074846929&doi=10.1002%2fsmtd.201900378&partnerID=40&md5=76166ba3cd141e642f28e3bdd
c580c0d
VL - 3
ID - 5443
ER -
TY - JOUR
AB - Background: Xixin has been widely used as a traditional Chinese medicine for
headache, toothache and inflammatory diseases. Clinical investigation indicated
that adverse drug reactions occurred with an overdose of xixin, but the toxic
mechanism of xixin in vivo based on trace elements has not been researched yet.
Objective: To explore the in vivo toxic mechanism of xixin induced by trace
elements. Materials and Methods: The contents of trace elements in the serum and
liver of mice were determined by inductively coupled plasma-mass spectrometry (ICP-
MS) after obtaining xixin extracts. Principal component analysis (PCA) and cluster
analysis (CA) were performed between the trace elements' content and dosage using
the software GeneSpring 12.1 to analyze the main toxic elements in vivo. Results:
Trace elements' contents were obviously raised after xixin extracts were taken as a
dosage of 150 mg/mL and 50 mg/mL, respectively. Na, Ca, Cu and Cd in serum and Ca
and Zn in liver were the main trace elements inducing the toxic reaction of xixin.
Conclusion: Xixin possesses the potential function of indirectly upregulating trace
elements in vivo. This study, for the first time, elucidated the in vivo toxic
mechanism of xixin based on trace elements. This method could also be utilized in
the research of corresponding aspects.
AU - Yong-Rui, B.
AU - Xin-Xin, Y.
AU - Shuai, W.
AU - Xian-Sheng, M.
AU - Rui-Qing, Z.
AU - Yue-Ming, X.
AU - Lin, C.
DB - Scopus
DO - 10.4103/0973-1296.131025
IS - 38
KW - Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag
cluster analysis
inductively coupled plasma-mass spectrometry
toxicity
aluminum
arsenic
beryllium
cadmium
calcium
Chinese drug
chromium
copper
iron
magnesium
manganese
potassium
selenium
silver
sodium
trace element
unclassified drug
vanadium
xixin
zinc
herbaceous agent
plant extract
xixin extract
adult
animal experiment
article
drug blood level
drug liver level
drug mechanism
female
flow rate
in vivo study
limit of detection
mass spectrometry
metabolism
mouse
nonhuman
peristaltic pump
animal model
animal tissue
Article
blood level
Chinese medicine
controlled study
data analysis software
liver level
M3 - Article
PY - 2014
SP - 141-146
ST - Study on the in vivo toxic mechanism of xixin based on trace elements
determination by inductively coupled plasma-mass spectrometry
T2 - Pharmacognosy Magazine
TI - Study on the in vivo toxic mechanism of xixin based on trace elements
determination by inductively coupled plasma-mass spectrometry
84899432295&doi=10.4103%2f0973-
1296.131025&partnerID=40&md5=731be72204c783043ad5fffcf632031d
VL - 10
ID - 5616
ER -
TY - JOUR
AB - Silver nanoparticles (Ag-NPs) have well-known antimicrobial properties and
have been widely applied as nano-strategies in myriad medical fields. Indeed, early
growth response (Egr-1) is an important transcription factor that has been
implicated in numerous inflammatory diseases. The administration of Ag-NPs
transiently increased the levels of Egr-1 mRNA in human keratinocytes with maximal
induction at the 30 minute time point. In an effort to identify molecular
signatures associated with cellular responses to Ag-NPs, we adopted an approach
involving gene expression profiling of human keratinocytes treated with or without
Egr-1 siRNA transfection using a cDNA microarray. The microarray analysis
demonstrated that: the expression profile of the genes involved in inflammation and
immune response were either stimulated or repressed. Overall, this study was
sufficient to reliably recognize the engagement of Egr-1-driven molecular signaling
pathways in Ag-NPs-treated keratinocytes.
AN - WOS:000338272300004
AU - Yoo, G.
AU - Jeong, S. H.
AU - Ryu, W. I.
AU - Lee, H.
AU - Kim, J. H.
AU - Bae, H. C.
AU - Son, S. W.
DA - JUN 30
DO - 10.1007/s13273-014-0016-9
IS - 2
PY - 2014
SN - 1738-642X
2092-8467
SP - 149-156
ST - Gene expression analysis reveals a functional role for the Ag-NPs-induced
Egr-1 transcriptional factor in human keratinocytes
T2 - MOLECULAR & CELLULAR TOXICOLOGY
TI - Gene expression analysis reveals a functional role for the Ag-NPs-induced
Egr-1 transcriptional factor in human keratinocytes
VL - 10
ID - 6262
ER -
TY - JOUR
AB - Ganoderma lucidum is a traditional Oriental medicine that has been widely
used as a tonic to promote longevity and health in Korea and other Asian countries.
Although a great deal of work has been carried out on the therapeutic potential of
this mushroom, the pharmacological mechanisms of its anti-inflammatory actions
remain unclear. In this study, we evaluated the inhibitory effects of G. lucidum
ethanol extract (EGL) on the production of inflammatory mediators and cytokines in
lipopolysaccharide (LPS)-stimulated murine BV2 microglia. We also investigated the
effects of EGL on the LPS-induced activation of nuclear factor kappaB (NF-kappa B)
and upregulation of toll-like receptor 4 (TLR4) and myeloid differentiation factor
88 (MyD88). Elevated levels of nitric oxide (NO), prostaglandin E-2 (PGE(2)) and
pro-inflammatory cytokine production were detected in BV2 microglia following LPS
stimulation. We identifed that EGL significantly inhibits the excessive production
of NO, PGE(2) and pro-inflammatory cytokines, including interleukin (IL)-1 beta and
tumor necrosis factor-alpha in a concentration-dependent manner without causing
cytotoxicity. In addition, EGL suppressed NF-kappa B translocation and
transcriptional activity by blocking I kappa B degradation and inhibiting TLR4 and
MyD88 expression in LPS-stimulated BV2 cells. Our results indicate that the
inhibitory effects of EGL on LPS-stimulated inflammatory responses in BV2 microglia
are associated with the suppression of the NF-kappa B and TLR signaling pathways.
Therefore, EGL may be useful in the treatment of neurodegenerative diseases by
inhibiting inflammatory mediator responses in activated microglia.
AN - WOS:000315423500056
AU - Yoon, H. M.
AU - Jang, K. J.
AU - Han, M. S.
AU - Jeong, J. W.
AU - Kim, G. Y.
AU - Lee, J. H.
AU - Choi, Y. H.
DA - MAR
DO - 10.3892/etm.2013.895
IS - 3
PY - 2013
SN - 1792-0981
1792-1015
SP - 957-963
ST - Ganoderma lucidum ethanol extract inhibits the inflammatory response by
suppressing the NF-kappa B and toll-like receptor pathways in lipopolysaccharide-
stimulated BV2 microglial cells
T2 - EXPERIMENTAL AND THERAPEUTIC MEDICINE
TI - Ganoderma lucidum ethanol extract inhibits the inflammatory response by
suppressing the NF-kappa B and toll-like receptor pathways in lipopolysaccharide-
stimulated BV2 microglial cells
VL - 5
ID - 6419
ER -
TY - JOUR
AB - Nanotechnology is a highly promising field, with nanoparticles produced and
utilized in a wide range of commercial products. Silver nanoparticles (AgNPs) has
been widely used in clothing, electronics, bio-sensing, the food industry, paints,
sunscreens, cosmetics and medical devices, all of which increase human exposure and
thus the potential risk related to their short- and long-term toxicity. Many
studies indicate that AgNPs are toxic to human health. Interestingly, the majority
of these studies focus on the interaction of the nano-silver particle with single
cells, indicating that AgNPs have the potential to induce the genes associated with
cell cycle progression, DNA damage and mitochondrial associated apoptosis. AgNPs
administered through any method were subsequently detected in blood and were found
to cause deposition in several organs. There are very few studies in rats and mice
involving the in vivo bio-distribution and toxicity, organ accumulation and
degradation, and the possible adverse effects and toxicity in vivo are only slowly
being recognized. In the present review, we summarize the current data associated
with the increased medical usage of nano-silver and its related nano-materials,
compare the mechanism of antibiosis and discuss the proper application of nano-
silver particles. © 2012 Springer Science+Business Media B.V.
AU - You, C.
AU - Han, C.
AU - Wang, X.
AU - Zheng, Y.
AU - Li, Q.
AU - Hu, X.
AU - Sun, H.
DB - Scopus
DO - 10.1007/s11033-012-1792-8
IS - 9
KW - Application
Mechanism
Angiogenesis Inhibitors
Animals
DNA Damage
Humans
Metal Nanoparticles
Silver
Mus
Rattus
interleukin 12
interleukin 8
molecular scaffold
oxygen
poly (3 hydroxybutyrate co 3 hydroxyvalerate)
silver nanoparticle
sulfadiazine silver
superoxide
titanium dioxide
unclassified drug
allergic reaction
antifungal activity
antiviral activity
apoptosis
biomedicine
burn
contact dermatitis
cytotoxicity
DNA damage
drug mechanism
embryotoxicity
gynecology
human
nanotechnology
oxidative stress
pharmacological blocking
reproduction
respiratory chain
review
surgical approach
toxicity
uterine cervicitis
uterine cervix erosion
vaginitis
wound dressing
wound infection
M3 - Review
PY - 2012
SP - 9193-9201
ST - The progress of silver nanoparticles in the antibacterial mechanism, clinical
application and cytotoxicity
T2 - Molecular Biology Reports
TI - The progress of silver nanoparticles in the antibacterial mechanism, clinical
application and cytotoxicity
84865137831&doi=10.1007%2fs11033-012-1792-
8&partnerID=40&md5=e8ecb4cba18f005b0f7b30c59916eabe
VL - 39
ID - 5663
ER -
TY - JOUR
AB - 目的：探讨含纳米银的胶原蛋白-丝素蛋白支架对大鼠全层皮肤缺损创面真皮再生的影响。方法：采用冷
冻干燥法分别制备 81 个含纳米银的胶原蛋白-丝素蛋白支架(纳米银质量浓度为 10 mg/L)及 81 个不含纳米银的
胶原蛋白-丝素蛋白支架，分别归为纳米银支架组和对照支架组。每组取 9 个支架加入人 Fb 培养，于培养
2、12、24 h 每组取 3 孔支架，计数 2 组支架区域黏附人 Fb。于 36 只 SD 大鼠背部各制作 4 个全层皮肤缺损创
面，将大鼠按随机数字表法平均分为纳米银支架组和对照支架组，创面分别移植含纳米银的胶原蛋白-丝素蛋白支
架和不含纳米银的胶原蛋白-丝素蛋白支架。于术后 1、2、4 周，每组分别取 6 只大鼠处死，对创面进行大体观察，
HE 染色观察组织结构、炎性细胞浸润及胶原沉积情况，免疫组织化学染色观察计数 CD68 阳性细胞，实时荧光定
量 RT-PCR 法检测创面 IL-6 和 IL-10 的 mRNA 表达。对数据行析因设计方差分析、t 检验及 Bonferroni 校正。
结果： (1)培养 2、12、24 h，纳米银支架组和对照支架组支架区域人 Fb 黏附数量相近(t 值为 1.77～
2.60，P 值均大于 0.05)。(2)术后 1 周，纳米银支架组大鼠创面及创缘未见明显感染迹象，支架血管化明显；
对照支架组大鼠创面出现明显感染迹象，小部分支架脱落。术后 2 周，纳米银支架组大鼠创面移植支架与创面贴
附牢固；对照支架组大鼠创面挛缩明显，大部分支架已脱落。术后 4 周，纳米银支架组大鼠创面移植支架已成功
覆盖创面，支架表面已明显上皮化；对照支架组大鼠创面移植支架完全脱落，残余创面明显收缩。(3)术后 1、2
周，与对照支架组比较，纳米银支架组大鼠创面胶原分泌及组织再生更多，支架内部炎性细胞浸润较少。术后 4
周，纳米银支架组大鼠创面已明显上皮化；而对照支架组大鼠创面仍有炎性细胞浸润，未见明显上皮化。(4)术后
1 周，纳米银支架组大鼠创面 CD68 阳性细胞数为(54±10)个/mm(2)，与对照支架组的(78±7)个/mm(2)无明显
差异(t＝1.52，P>0.05)。术后 2、4 周，纳米银支架组大鼠创面 CD68 阳性细胞数分别为(154±10)、(77±7)
个/mm(2)，均明显少于对照支架组的(268±16)、(136±13)个/mm(2)(t 值分别为 7.31 和 3.83，P 值均小于
0.01)。(5)除术后 4 周(t＝1.23，P>0.05)外，术后 1、2 周纳米银支架组大鼠创面 IL-6 的 mRNA 表达量均明
显低于对照支架组(t 值分别为 13.12 和 4.65，P 值均小于 0.01)。除术后 1 周(t＝3.08，P<0.05)外，术后
2、4 周 2 组大鼠创面 IL-10 的 mRNA 表达量均相近(t 值分别为 2.14 和 0.49，P 值均大于 0.05)。结论：含
纳米银的胶原蛋白-丝素蛋白支架不仅具有良好的生物相容性，同时具有明显的炎症调节作用，可加快胶原蛋白-
丝素蛋白支架诱导真皮再生，促进创面修复。.; Objective: To explore the influence of
collagen/fibroin scaffolds containing silver nanoparticles on dermal regeneration
of full-thickness skin defect wound in rat. Methods: Eighty-one collagen/fibroin
scaffolds containing silver nanoparticles (with the mass concentration of silver
nanoparticles as 10 mg/L) and 81 collagen/fibroin scaffolds without silver
nanoparticles were produced respectively with freeze-drying method and enrolled as
silver nanoparticles scaffold group (SNS) and control scaffold group (CS). Nine
scaffolds in each group were cultured with human fibroblasts. At post culture hour
(PCH) 2, 12, and 24, the human fibroblasts adherent to the scaffolds (n=3) in two
groups were counted. Four full-thickness skin defect wounds were reproduced on the
back of each one of the 36 SD rats. The rats were divided into groups SNS (wounds
were transplanted with collagen/fibroin scaffolds containing silver nanoparticles)
and CS (wounds were transplanted with collagen/fibroin scaffolds without silver
nanoparticles) according to the random number table, with 18 rats in each group. In
ost surgery week (PSW) 1, 2, and 4, 6 rats in each group were sacrificed
respectively for general observation, observation of histological structure,
inflammatory cell infiltration, and collagen deposition with HE staining, count of
CD68 positive cells with immunohistochemical staining, and mRNA expressions of
interleukin-6 (IL-6) and IL-10 with real-time fluorescent quantitative reverse
transcription polymerase chain reaction. Data were processed with analysis of
variance of factorial design, t test, and Bonferroni correction. Results: (1) At
PCH 2, 12, and 24, the numbers of human fibroblasts adherent to the scaffolds in
the two groups were close (with t values from 1.77 to 2.60, P values above 0.05).
(2) In PSW 1, no obvious symptom of infection was observed in wound or wound edge
of rats in group SNS with obvious vascularization of scaffolds, while obvious
symptoms of infection were observed in wounds of rats in group CS with some
scaffolds exfoliated. In PSW 2, the scaffolds were firmly attached to the wounds of
rats in group SNS, while obvious contracture was observed in the wounds of rats in
group CS with a lot of scaffolds exfoliated. In PSW 4, the scaffolds covered the
wounds of rats in group SNS with obvious epithelization on the surface of the
scaffolds, while all the scaffolds exfoliated, leaving obvious contracture of
residual wounds of rats in group CS. (3) In PSW 1 and 2, compared with those in
group CS, more collagen secretion and tissue regeneration and less inflammatory
cell infiltration in the scaffolds were observed in the wounds of rats in group
SNS. In PSW 4, obvious epithelization was observed in the wounds of rats in group
SNS, while inflammatory cell infiltration was observed without obvious
epithelization in the wounds of rats in group CS. (4) In PSW 1, the number of CD68
positive cells in the wounds of rats in group SNS [(54±10) /mm(2)] was similar to
that in group CS [(78±7) /mm(2,) t=1.52, P>0.05]. In PSW 2 and 4, the numbers of
CD68 positive cells in the wounds of rats in group SNS [(154±10) and (77±7) /mm(2)]
were significantly less than those in group CS [(268±16) and (136±13) /mm(2,) with
t values respectively 7.31 and 3.83, P values below 0.01] respectively. (5) Except
for the expression in PSW 4 (t=1.23, P>0.05), the mRNA expressions of IL-6 in the
wounds of rats in group SNS in PSW 1 and 2 were significantly lower than those in
group CS (with t values respectively 13.12 and 4.65, P values below 0.01). Except
for the expression in PSW 1 (t=3.08, P<0.05), the mRNA expressions of IL-10 in PSW
2 and 4 in the wounds of rats in the two groups were similar (with t values
respectively 2.14 and 0.49, P values above 0.05). Conclusions: Besides good
biocompatibility, collagen/fibroin scaffolds containing silver nanoparticles have
obvious effect in modulating inflammation, thus they can accelerate dermal
regeneration induced by collagen/fibroin scaffolds for wound repair.
AU - You, Z. G.
AU - Zhang, L. P.
AU - Wang, X. G.
AU - Zhou, H. L.
AU - Guo, S. X.
AU - Wu, P.
AU - Han, C. M.
DB - Scopus
DO - 10.3760/cma.j.issn.1009-2587.2017.02.011
IS - 2
KW - Dermal regeneration
Inflammation
Nanomedicine
Tissue scaffolds
Animals
Collagen
Fibroins
Humans
Interleukin-10
Interleukin-6
Nanoparticles
Rats
Regeneration
Silver
Skin
Skin Abnormalities
Treatment Outcome
Wound Healing
collagen
fibroin
IL10 protein, human
IL6 protein, human
interleukin 10
interleukin 6
nanoparticle
silver
animal
congenital skin disease
drug effects
human
rat
regeneration
skin
soft tissue injury
Sprague Dawley rat
treatment outcome
wound healing
M3 - Article
PY - 2017
SP - 103-110
ST - Influence of collagen/fibroin scaffolds containing silver nanoparticles on
dermal regeneration of full-thickness skin defect wound in rat
T2 - Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of
burns
TI - Influence of collagen/fibroin scaffolds containing silver nanoparticles on
dermal regeneration of full-thickness skin defect wound in rat
85021859023&doi=10.3760%2fcma.j.issn.1009-
2587.2017.02.011&partnerID=40&md5=5211426889b41d1408d5556fd0fe458d
VL - 33
ID - 5494
ER -
TY - JOUR
AB - Increasing evidence has demonstrated that amyloid-beta peptide (A beta), the
hallmark of Alzheimer's disease (AD), evokes oxidative and inflammatory cascades,
which ultimately lead to the death of neurons. The purpose of the present study is
to demonstrate the effect of nobiletin, a representative compound of citrus peel,
in preventive and therapeutic approaches against neuronal damage by exposure to A
beta(25-35). Nobiletin significantly ameliorated A beta(25-35-)mediated cell death
by restoring abnormal changes in intracellular oxidative stress, cell cycle,
nuclear morphology, and activity of apoptotic caspase. Regarding anti-inflammatory
responses, nobiletin significantly suppressed interleukin-1 beta, tumor necrosis
factor-alpha, nitric oxide (NO), and prostaglandin E-2 production in response to A
beta stimulation. Moreover, nobiletin inhibited A beta-stimulated inducible NO
synthase and cyclooxygenase-2 expression, which was attributed to the blockade of
nuclear factor-kappa B p65 and phosphorylation of its inhibitor, I kappa B-alpha.
Interestingly, nobiletin decreased expression of c-Jun N-terminal kinase and p38
without affecting extracellular signal-regulated kinase 1/2 activation. Taken
together, the novel data implicate nobiletin as a potential candidate for the
prevention of AD through the inhibition of oxidative stress, apoptosis, and
inflammation.
AN - WOS:000502274600099
AU - Youn, K.
AU - Lee, S.
AU - Jun, M.
C7 - 2648
DA - NOV
DO - 10.3390/nu11112648
IS - 11
PY - 2019
SN - 2072-6643
ST - Discovery of Nobiletin from Citrus Peel as a Potent Inhibitor of beta-Amyloid
Peptide Toxicity
T2 - NUTRIENTS
TI - Discovery of Nobiletin from Citrus Peel as a Potent Inhibitor of beta-Amyloid
Peptide Toxicity
VL - 11
ID - 6462
ER -
TY - JOUR
AB - Cardiovascular disease (CVD) is the leading cause of death worldwide. A
search for more effective treatments of CVD is increasingly needed. Major advances
in nanotechnology opened new avenues in CVD therapeutics. Owing to their special
properties, iron oxide, gold and silver nanoparticles (NPs) could exert various
effects in the management and treatment of CVD. The role of iron oxide NPs in the
detection and identification of atherosclerotic plaques is receiving increased
attention. Moreover, these NPs enhance targeted stem cell delivery, thereby
potentiating the regenerative capacity at the injured sites. In addition to their
antioxidative and antihypertrophic capacities, gold NPs have also been shown to be
useful in the identification of plaques and recognition of inflammatory markers.
Contrary to first reports suggestive of their cardio-vasculoprotective role, silver
NPs now appear to exert negative effects on the cardiovascular system. Indeed,
these NPs appear to negatively modulate inflammation and cholesterol uptake, both
of which exacerbate atherosclerosis. Moreover, silver NPs may precipitate
bradycardia, conduction block and sudden cardiac death. In this review, we dissect
the cellular responses and toxicity profiles of these NPs from various perspectives
including cellular and molecular ones. © 2021 The Authors
AU - Younis, N. K.
AU - Ghoubaira, J. A.
AU - Bassil, E. P.
AU - Tantawi, H. N.
AU - Eid, A. H.
C7 - 102433
DB - Scopus
DO - 10.1016/j.nano.2021.102433
Cardiovascular disease
Gold nanoparticles
Iron oxide nanoparticles
Nanomedicine
Biomarkers
Cardiovascular Diseases
Ferric Compounds
Gold
Humans
Metal Nanoparticles
Silver
Cardiology
Iron oxides
Metal nanoparticles
Pathology
Stem cells
beta adrenergic receptor blocking agent
biological marker
chitosan derivative
contrast medium
dextran derivative
dipeptidyl carboxypeptidase inhibitor
ferumoxytol
gadolinium
gold nanoparticle
hydroxymethylglutaryl coenzyme A reductase inhibitor
macrogol
metal nanoparticle
silver nanoparticle
superparamagnetic iron oxide
ferric ion
ferric oxide
gold
silver
Atherosclerotic plaque
Cholesterol uptakes
Detection and identifications
Regenerative capacity
Special properties
Sudden cardiac deaths
atherosclerosis
bradycardia
cardiotoxicity
cholesterol transport
drug coating
drug cytotoxicity
heart block
heart failure
heart protection
heart ventricle remodeling
human
iron deficiency anemia
liver toxicity
mesenchymal stem cell transplantation
nanomedicine
nanotechnology
nonhuman
regenerative ability
Review
risk factor
sudden cardiac death
blood
Diseases
M3 - Review
PY - 2021
ST - Metal-based nanoparticles: Promising tools for the management of
cardiovascular diseases
TI - Metal-based nanoparticles: Promising tools for the management of
cardiovascular diseases
85110052474&doi=10.1016%2fj.nano.2021.102433&partnerID=40&md5=9ae0ce6f54f2388f68de4
72383a0a627
VL - 36
ID - 5209
ER -
TY - JOUR
AB - Background. Silver nanoparticles (AgNPs) utilization is becoming increasingly
popular. The existing investigation evaluates the ameliorative impact of eugenol
(Eug) against the toxic influences of AgNPs on rats' liver. Methods. Sixty adult
male rats were enrolled equally into control, Eug (100 mg kg-1 orally), AgNPs-low
dose (1 mg kg-1 i.p), AgNPs-high dose (2 mg kg-1 i.p), Eug + AgNPs-low dose (100 mg
kg-1 orally+1 mg kg-1 i.p), and Eug + AgNPs high dose (100 mg kg-1 orally+2 mg kg-1
i.p). All the groups were treated daily for 30 days, subsequently serum aspartate
transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total
protein, total albumin, lactate dehydrogenase (LDH), total oxidative capacity
(TOC), malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-α), total
antioxidant capacity (TAC), and interleukin 6 (IL-6) levels were measured; hepatic
tissues superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and
glutathione peroxidase (GPx) levels were evaluated; histopathology and
histomorphometry were documented in the liver of all groups; and Bcl-2, P53,
Caspase-3, and TNF-α reactive proteins were also immunohistochemically detected.
Results. AgNPs significantly triggered oxidative stress in hepatic tissues,
characterized by elevated levels of AST, ALT, ALP, LDH, TOC, MDA, TNF-α, and IL-6
correlating with considerable decline in total protein, total albumin, TAC, SOD,
CAT, GSH, and GPx. These changes were paralleled with histopathological alterations
remarkable by devastation of the ordinary hepatic structure, with decrease in the
numbers of normal hepatocytes, elevation in the numbers of necrotic hepatocytes,
periportal and centrilobular inflammatory cells, deteriorated Kupffer cells, and
dilated/congested central and portal veins. Alongside, a marked diminution in Bcl-2
immunoreactivity and a significant elevation in P53, Caspase-3, and TNF-α
immunoreactivities were recorded. Supplementation of AgNPs-treated animals with Eug
reversed most of the biochemical, histopathological, and immunohistochemical
changes. Conclusion. This study proposed that Eug has an ameliorative effect
against AgNPs-induced hepatotoxicity. © 2022 Hany N. Yousef et al.
AU - Yousef, H. N.
AU - Ibraheim, S. S.
AU - Ramadan, R. A.
AU - Aboelwafa, H. R.
C7 - 3820848
DB - Scopus
DO - 10.1155/2022/3820848
KW - Alanine
Capacity
Cells
Control Systems
Dosage
Eugenol
Silver
Stresses
Albumins
Alkaline Phosphatase
Animals
Antioxidants
Aspartate Aminotransferases
Caspase 3
Catalase
Glutathione
Glutathione Peroxidase
Interleukin-6
Lactate Dehydrogenases
Male
Malondialdehyde
Metal Nanoparticles
Rats
Rats, Wistar
Alkalinity
Amino acids
Cell death
Histology
Liver
Metal nanoparticles
Peptides
Phosphatases
Plants (botany)
Superoxide dismutase
Tissue
Toxicity
albumin
caspase 3
catalase
eugenol
glutathione
interleukin 6
malonaldehyde
protein bcl 2
protein p53
silver nanoparticle
albuminoid
antioxidant
metal nanoparticle
silver
ALkaline phosphatase
Aspartates
Hepatotoxicity
High dose
Lactate dehydrogenase
Low dose
Oxidative capacity
TNF α
Total protein
animal cell
animal experiment
animal model
animal tissue
Article
controlled study
gene expression
hepatic portal vein
histopathology
immunoreactivity
inflammatory cell
Kupffer cell
lactate blood level
liver function
liver protection
liver structure
liver tissue
liver toxicity
male
morphometry
nonhuman
oxidative stress
physical chemistry
protein expression
rat
surface property
total antioxidant capacity
Wistar rat
X ray diffraction
animal
metabolism
toxic hepatitis
M3 - Article
PY - 2022
ST - The Ameliorative Role of Eugenol against Silver Nanoparticles-Induced
Hepatotoxicity in Male Wistar Rats
T2 - Oxidative Medicine and Cellular Longevity
TI - The Ameliorative Role of Eugenol against Silver Nanoparticles-Induced
Hepatotoxicity in Male Wistar Rats
85138188924&doi=10.1155%2f2022%2f3820848&partnerID=40&md5=35a6387ecb8cb1f3f143ec6c3
dc4693e
VL - 2022
ID - 5061
ER -
TY - JOUR
AB - Purpose: Mesoporous silica (MSNs) have attracted considerable attention for
its application in the field of drug delivery and biomedicine due to its high
surface area, large pore volume, and low toxicity. Recently, numerous studies
revealed that gut microbiota is of critical relevance to host health. However, the
toxicological studies of MSNs were mainly based on the degradation,
biodistribution, and excretion in mammalian after oral administration for now. Here
in this study, we explored the impacts of oral administration of three kinds of
MSNs on gut microbiota in rats to assess its potential toxicity. Methods: Forty
rats were divided into four groups: control group; Mobil Composition of Matter No.
41 type mesoporous silica (MCM-41) group; Santa Barbara Amorphous-15 type
mesoporous silica (SBA-15) group, and biodegradable dendritic center-radial
mesoporous silica nanoparticle (DMSN) group. Fecal samples were collected 3 days
and 7 days after the intake of MSNs and analyzed with high throughput sequencing.
Gastric tissues in rats were obtained after dissection for the histological study.
Results: Three different MSNs (MCM-41, SBA-15, and DMSN) were successfully prepared
in this study. The pore size of three MSNs was calculated similarly as (3.54 +/-
0.15) nm, (3.48 +/- 0.21) nm, and (3.45 +/- 0.17) nm according to the BET & BJH
model, respectively, while the particle size of MCM-41, SBA-15 and DMSN was around
209.2 nm, 1349.56 nm, and 244.4 nm, respectively. In the gene analysis of 16S rRNA,
no significant changes in the diversity and richness were found between groups,
while Verrucomicrobia decreased and Candidatus Saccharibacteria increased in MCM-41
treated groups. Meanwhile, no inflammatory and erosion symptoms were observed in
the morphological analysis of the colons, except the MCM-41 treated group.
Conclusion: Three different MSNs, MCM-41, SBA-15, and DMSN were successfully
prepared, and this study firstly suggested the impact of MSNs on the gut
microbiota, and further revealing the potential pro-inflammatory effects of oral
administration of MCM-41 was possibly through the changing of gut microbiota.
AN - WOS:000614621800001
AU - Yu, Y.
AU - Wang, Z.
AU - Wang, R.
AU - Jin, J.
AU - Zhu, Y. Z.
DO - 10.2147/IJN.S295575
PY - 2021
SN - 1178-2013
SP - 881-893
ST - Short-Term Oral Administration of Mesoporous Silica Nanoparticles Potentially
Induced Colon Inflammation in Rats Through Alteration of Gut Microbiota
TI - Short-Term Oral Administration of Mesoporous Silica Nanoparticles Potentially
Induced Colon Inflammation in Rats Through Alteration of Gut Microbiota
VL - 16
ID - 6738
ER -
TY - JOUR
AB - A histologie study was made of tho response of the leptomeninges and
underlying cerebral cortex of the cat to subdural implantation of 3 insulating
materials (HR605-P, Parylene* * Trade name of Union Carbide Corp.-C and PI-2555)
and a polymeric electrode component (MMA/MAPTAC) for periods of 8 and 16 wk. The
tissue reactions were compared with those elicited by the arrays of Dacron† †
Dacron® is a registered trade name of Dupont Chemical Corp. mesh matrices, pure
platinum controls and by positive controls (Ag-AgCI) known to cause reactions in
the brain. Sites beneath the Dacron mesh matrix, pure platinum control implants and
beneath all insulating materials implanted for 8 and 16 wk appeared
indistinguishable, exhibiting little tissue reaction. All neurons appeared normal.
The leptomeninges and cortex beneath the Ag-AgCI implants showed a chronic
inflammatory reaction after 8 and 16 wk. Despite varying amounts of oedema, gliosis
and ingrowth of connective tissue in the molecular layer, virtually all underlying
neurons appeared normal. © 1987.
AU - Yuen, T. G. H.
AU - Agnew, W. F.
AU - Bullara, L. A.
DB - Scopus
DO - 10.1016/0142-9612(87)90103-7
IS - 2
Implants
neuroprosthetic implants
nonporous polymers
silver chloride
subdural space
Animal
Brain
Cats
Cerebral Cortex
Female
Male
Meninges
Polymers
Subdural Space
celluloid
dacron
hr 605p
methacrylic acid methyl ester
parylene c
platinum
unclassified drug
animal experiment
brain cortex
cat
drug tolerance
electrode
histology
leptomeninx
nervous system
nonhuman
M3 - Article
PY - 1987
SP - 138-141
ST - Tissue response to potential neuroprosthetic materials implanted subdurally
T2 - Biomaterials
TI - Tissue response to potential neuroprosthetic materials implanted subdurally
0023141992&doi=10.1016%2f0142-9612%2887%2990103-
7&partnerID=40&md5=0b32564d4f49cc3cca939cd8b3e52f0b
VL - 8
ID - 5789
ER -
TY - JOUR
AB - Introduction: CD4+CD25+ Foxp3+ T regulatory cell (Tregs) represents
approximately 8-10% of the total CD4+ T cell population and are important for
immune homeostasis and preventing autoimmune development. Thus, harnessing their
functions as immune modulator may be coupled with the rapid advancement of
nanotechnology development. Plant-mediated biosynthesis of silver nanoparticle
(AgNP) is noteworthy due to simplicity, rapid rate and potentially render more
biocompatibility with biomolecules. This study identified the effect of
biosynthesized-AgNPs from Garcinia atroviridis (GA) in modulating inflammatory
properties of Treg cells in Non-Obese Resistant (NOR). GA extract was used to
biosynthesized AgNPs and was tested on the effect of inducing inflammatory
properties in CD4+IL17Rhigh cells following 72hr in vitro treatment. Methods:
Conventional CD4+CD25-Foxp3- cells from female NOR mice were sorted using magnetic
separation and cultured in RPMI in the presence of anti-CD3/CD28 antibodies, TGF-β
and IL-2 cytokines. Cells were then treated with or without GA-AgNPs for 48hr of
iTreg cell induction and then re-cultured with new media treated with respective
treatments received. After 72hr in vitro culture, cells were stained with
fluorochrome-conjugated antibodies for flow cytometry. Results: Current result
showed that AgNPs suppress CD4 expression in CD4+IL17Rhigh population. MAPK pathway
proteins remain unchanged in both control and AgNP-treated groups. Conclusions: The
preliminary findings may suggest the properties of GA-AgNPs in modulating CD4+ T
cell population in normal condition. Further studies are necessary to elucidate the
molecular mechanisms involve in such interaction. Current findings serve as basis
in further identifying the immunomodulatory profile of nanoparticle for potential
therapeutic use. © 2018 UPM Press. All rights reserved.
AU - Yusof, N. A. M.
AU - Lee, P. C.
AU - Kamal, N. N. S. N. M.
AU - Zulkifli, N. I.
AU - Ahmad, N. H.
AU - Omar, W. A. W.
AU - Mohamed, R.
AU - Effa, S. Z. N.
DB - Scopus
KW - Gardinia atriviridis
Immunomodulator
Nanoparticle
Pro-inflammatory
T-regulatory cells
M3 - Article
PY - 2018
SP - 88-94
ST - Antagonistic effect of biosynthesized AgNPs from garcinia
atroviridisextractonanti-inflammatorypropertiesoFCD4+Ilrhigh cells from non obese
resistance (NOR) mouse model
T2 - Malaysian Journal of Medicine and Health Sciences
TI - Antagonistic effect of biosynthesized AgNPs from garcinia
atroviridisextractonanti-inflammatorypropertiesoFCD4+Ilrhigh cells from non obese
resistance (NOR) mouse model
85068605717&partnerID=40&md5=095452659d6f7bf2b5fcc8b99c38063d
VL - 14
ID - 5517
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) are widely used in a variety of consumable
products as antibacterial to prevent or treat infection. Unfortunately, evidence
exits that AgNP induces inflammation which can worsen with repeated human exposure.
However, there is little or no research on how to mitigate these adverse effects
due to AgNP induced-toxicity. Here, we investigated if surface modification of AgNP
by liposomal encapsulation suppresses AgNP-mediated inflammatory responses in THP1
monocytes and THP1 differentiated macrophages (TDM). AgNP was encapsulated in a
dipalmitoyl phosphatidyl choline- (DPPC)/cholesterol-based liposome by extrusion
through a 100-nm polycarbonate membrane to form Lipo-AgNP. It was found as expected
that AgNP induced significant release of IL-1β, IL-6, IL-8 and TNF-α in THP1
monocytes more than the basal level. Interestingly, release of these cytokines was
suppressed by Lipo-AgNP. In TDMs, AgNP and Lipo-AgNP induced IL-8 release (p
< .0001), but Lipo-AgNP maintained IL-8 release at levels significantly lower than
that of AgNP (p < .01). However, both AgNP and Lipo-AgNP suppressed IL-1β and TNF-α
release in LPS-stimulated THP1 monocytes and LPS-stimulated or unstimulated TDM
respectively. We finally showed that Lipo-AgNP inhibits STAT-3 and this may be
responsible for regulating the uncontrolled inflammation induced by AgNP likely
mediated STAT-3 protein expression in LPS stimulated THP1 monocytes and TDMs, both
LPS-stimulated and unstimulated. This data showed that Lipo-AgNP suppressed AgNP
induced inflammation, making Lipo-AgNP particularly useful in treatment of bacteria
induced inflammatory diseases and inflammatory cancers. © 2019 Elsevier Ltd
AU - Yusuf, A.
AU - Casey, A.
C7 - 104641
DB - Scopus
DO - 10.1016/j.tiv.2019.104641
KW - AgNP
IL-1β
Inflammation
Liposomal-AgNP
TNF-α
Cytokines
Humans
Liposomes
Metal Nanoparticles
Silver
STAT3 Transcription Factor
THP-1 Cells
interleukin 1beta
interleukin 6
interleukin 8
silver nanoparticle
STAT3 protein
cytokine
liposome
metal nanoparticle
silver
STAT3 protein, human
Article
cell viability
controlled study
cytokine release
encapsulation
endothelium cell
human
human cell
hydrodynamics
inflammation
liposome membrane
macrophage
monocyte
neutrophil
protein expression
stroma cell
zeta potential
chemistry
metabolism
THP-1 cell line
M3 - Article
PY - 2019
ST - Surface modification of silver nanoparticle (AgNP) by liposomal encapsulation
mitigates AgNP-induced inflammation
TI - Surface modification of silver nanoparticle (AgNP) by liposomal encapsulation
mitigates AgNP-induced inflammation
85072019546&doi=10.1016%2fj.tiv.2019.104641&partnerID=40&md5=f586a561ec233f97bfc069
c6cb7d9bda
VL - 61
ID - 5399
ER -
TY - JOUR
AB - Dentists used silver-containing solutions for deep cavity disinfection before
restoration. This review aims to identify the silver-containing solutions reported
in the literature for deep cavity disinfection and summarize their effects on
dental pulp. An extensive search was performed using the search words "(silver) AND
(dental pulp OR pulp)" in ProQuest, PubMed, SCOPUS, and Web of Science to identify
English publications on silver-containing solutions for cavity conditioning. The
pulpal response to the included silver-containing solutions was summarized. The
initial search identified 4112 publications and 14 publications met the inclusion
criteria. Silver fluoride, silver nitrate, silver diamine nitrate, silver diamine
fluoride, and nano-silver fluoride were used in deep cavities for antimicrobial
purposes. Indirect silver fluoride application induced pulp inflammation and
reparative dentine in most cases, and pulp necrosis in some cases. Direct silver
nitrate application caused blood clots and a wide inflammatory band in the pulp,
whilst indirect silver nitrate application caused hypoplasia in shallow cavities
and partial pulp necrosis in deep cavities. Direct silver diamine fluoride
application induced pulp necrosis, while indirect silver diamine fluoride
application induced a mild inflammatory response and reparative dentine formation.
No evidence of the dental pulpal response to silver diamine nitrate or nano-silver
fluoride was available in the literature.
AN - WOS:001018283400001
AU - Zaeneldin, A.
AU - Chu, C. H.
AU - Yu, O. Y.
C7 - 114
DA - APR 26
DO - 10.3390/dj11050114
IS - 5
PY - 2023
SN - 2304-6767
ST - Dental Pulp Response to Silver-Containing Solutions: A Scoping Review
T2 - DENTISTRY JOURNAL
TI - Dental Pulp Response to Silver-Containing Solutions: A Scoping Review
VL - 11
ID - 6328
ER -
TY - JOUR
AB - Nanotechnology studies the various phenomena of physio-chemical procedures
and biological properties for the generation of nanosized particles, and their
rising challenges in the various sectors, like medicine, engineering, agriculture,
electronic, and environmental studies. The nanosized particles exhibit good anti-
microbial, anti-inflammatory, cytotoxic, drug delivery, anti-parasitic, anti-
coagulant and catalytic properties because of their unique dimensions with large
surface area, chemical stability and higher binding density for the accumulation of
various bio-constituents on their surfaces. Biological approaches for the synthesis
of silver nanoparticles (AgNPs) have been reviewed because it is an easy and
single-step protocol and a viable substitute for the synthetic chemical-based
procedures. Physical and chemical approaches for the production of AgNPs are also
mentioned herein. Biological synthesis has drawn attention because it is cost-
effective, faster, non-pathogenic, environment-friendly, easy to scale-up for
large-scale synthesis, and having no demand for usage of high pressure, energy,
temperature, or noxious chemical ingredients, and safe for human therapeutic use.
Therefore, the collaboration of nanomaterials with bio-green approaches could
extend the utilization of biological and cytological properties compatible with
AgNPs. In this perspective, there is an immediate need to develop eco-friendly and
biocompatible techniques, which strengthen efficacy against microbes and minimize
toxicity for human cells. The present study introduces the biological synthesis of
silver nanoparticles, and their potential biomedical applications have also been
reviewed. © 2021 Bentham Science Publishers.
AU - Zafar, S.
AU - Zafar, A.
AU - Jabeen, F.
AU - Ali Siddiq, M.
DB - Scopus
DO - 10.2174/2213346109666211217091042
IS - 3
KW - antimicrobial
biological methods
cytotoxicity
plant extract
synthetic approach
M3 - Review
PY - 2021
SP - 222-241
ST - Biological Synthesis of Silver Nanoparticles and their Biomedical Activity: A
Review
T2 - Current Green Chemistry
TI - Biological Synthesis of Silver Nanoparticles and their Biomedical Activity: A
Review
85163983818&doi=10.2174%2f2213346109666211217091042&partnerID=40&md5=109f65449ea80b
cb8182269fa330654a
VL - 8
ID - 5244
ER -
TY - JOUR
AB - Nanotechnology studies the various phenomena of physio-chemical procedures
and biological properties for the generation of nanosized particles, and their
rising challenges in the various sectors, like medicine, engineering, agriculture,
electronic, and environmental studies. The nanosized particles exhibit good anti-
microbial, anti-inflammatory, cytotoxic, drug delivery, anti-parasitic, anti-
coagulant and catalytic properties because of their unique dimensions with large
surface area, chemical stability and higher binding density for the accumulation of
various bio-constituents on their surfaces. Biological approaches for the synthesis
of silver nanoparticles (AgNPs) have been reviewed because it is an easy and
single-step protocol and a viable substitute for the synthetic chemical-based
procedures. Physical and chemical approaches for the production of AgNPs are also
mentioned herein. Biological synthesis has drawn attention because it is cost-
effective, faster, non-pathogenic, environment-friendly, easy to scale-up for
large-scale synthesis, and having no demand for usage of high pressure, energy,
temperature, or noxious chemical ingredients, and safe for human therapeutic use.
Therefore, the collaboration of nanomaterials with bio-green approaches could
extend the utilization of biological and cytological properties compatible with
AgNPs. In this perspective, there is an immediate need to develop eco-friendly and
biocompatible techniques, which strengthen efficacy against microbes and minimize
toxicity for human cells. The present study introduces the biological synthesis of
silver nanoparticles, and their potential biomedical applications have also been
reviewed.
AN - WOS:000734619200005
AU - Zafar, S.
AU - Zafar, A.
AU - Jabeen, F.
AU - Siddiq, M. A.
DO - 10.2174/2213346109666211217091042
IS - 3
PY - 2021
SN - 2213-3461
2213-347X
SP - 222-241
ST - Biological Synthesis of Silver Nanoparticles and their Biomedical Activity: A
Review
T2 - CURRENT GREEN CHEMISTRY
TI - Biological Synthesis of Silver Nanoparticles and their Biomedical Activity: A
Review
VL - 8
ID - 5912
ER -
TY - JOUR
AB - Due to their unique physicochemical properties, engineered nanoparticles
(NPs) are used in numerous skin products as sunscreen, texture agents, colorant,
and drug delivery systems. While the skin is considered the first line of defense
against xenobiotic entrance, the small size of NPs could promote the interaction
with cutaneous cells. This review investigates the fate and the toxicological
effects of organic and inorganic NPs used in cosmetic and dermatology. After direct
exposure to skin cells, cytotoxicity, oxidative stress, inflammatory response, and
genotoxicity were reported in a dose and time-dependent manner, especially for
inorganic NPs. Despite these findings, the toxicity of nanoparticles applied to a
healthy skin could be questioned due to their inability, for most of them, to reach
the viable epidermis. Advanced skin models and toxicity tests validated for
nanomaterials should be required for a better prediction of the dermal
nanotoxicity. © 2022, The Author(s), under exclusive licence to Springer Nature
B.V.
AU - Zaiter, T.
AU - Cornu, R.
AU - El Basset, W.
AU - Martin, H.
AU - Diab, M.
AU - Béduneau, A.
C7 - 149
DB - Scopus
DO - 10.1007/s11051-022-05523-2
IS - 7
KW - 3D skin models
Cytotoxicity
Genotoxicity
Inflammation
Nanoparticles
Skin barrier
Drug delivery
Textures
antiinfective agent
cytokine
gold nanoparticle
nanoparticle
nickel nanoparticle
silica nanoparticle
silver nanoparticle
3d skin model
Genotoxicities
Skin model
Toxicity assessment
cytotoxicity
dermatitis
ex vivo study
genotoxicity
human
in vitro study
inflammation
keratinocyte
nonhuman
oxidative stress
Review
skin cell
skin penetration
skin toxicity
time factor
toxicity testing
M3 - Review
PY - 2022
ST - Toxicity assessment of nanoparticles in contact with the skin
TI - Toxicity assessment of nanoparticles in contact with the skin
85133921597&doi=10.1007%2fs11051-022-05523-
2&partnerID=40&md5=e1c70e5f232ac383591bfa8a749d155e
VL - 24
ID - 5066
ER -
TY - JOUR
AB - Due to their unique physicochemical properties, engineered nanoparticles
(NPs) are used in numerous skin products as sunscreen, texture agents, colorant,
and drug delivery systems. While the skin is considered the first line of defense
against xenobiotic entrance, the small size of NPs could promote the interaction
with cutaneous cells. This review investigates the fate and the toxicological
effects of organic and inorganic NPs used in cosmetic and dermatology. After direct
exposure to skin cells, cytotoxicity, oxidative stress, inflammatory response, and
genotoxicity were reported in a dose and time-dependent manner, especially for
inorganic NPs. Despite these findings, the toxicity of nanoparticles applied to a
healthy skin could be questioned due to their inability, for most of them, to reach
the viable epidermis. Advanced skin models and toxicity tests validated for
nanomaterials should be required for a better prediction of the dermal
nanotoxicity.
AN - WOS:000824906200002
AU - Zaiter, T.
AU - Cornu, R.
AU - El Basset, W.
AU - Martin, H.
AU - Diab, M.
AU - Beduneau, A.
C7 - 149
DA - JUL
DO - 10.1007/s11051-022-05523-2
IS - 7
PY - 2022
SN - 1388-0764
1572-896X
ST - Toxicity assessment of nanoparticles in contact with the skin
TI - Toxicity assessment of nanoparticles in contact with the skin
VL - 24
ID - 6315
ER -
TY - JOUR
AB - Driven by the need to biosynthesize alternate biomedical agents to prevent
and treat infection, silver nanoparticles have surfaced as a promising avenue.
Cyanobacteria-derived nanomaterial synthesis is of substantive interest as it
offers an eco-friendly, cost-effective, sustainable, and biocompatible route for
further development. In the present study optimal conditions for synthesis of
silver nanoparticles (AgNPs) were 1 : 9 v/v [cell extract: AgNO3 (1 mM)], pH 7.4,
and 30 °C reaction temperatures. Synthesis of nanoparticles was monitored by UV-vis
spectrophotometry and the maximum absorbance was observed at a wavelength of 420
nm. SEM with EDX analysis confirmed 96.85% silver by weight which revealed the
purity of AgNPs. TEM & XRD analysis exhibited a particle size of ∼12 nm with
crystalline nature. FTIR analysis confirmed the presence of possible biomolecules
involved in the synthesis and stabilization of AgNPs. Decapping of AgNPs followed
by SDS-PAGE, LCMS and MALDI TOF analysis elucidates the proteinaceous nature of the
capping and stabilizing agent. Cyanobacterial-derived capped AgNPs showed more
cytotoxicicity towards a non-small cell lung cancer (A549) cell line, free radical
scavenger and an antimicrobial than de-capped AgNPs. In addition they showed
significant synergistic characteristics with antibiotics and fungicides. The test
revealed that the capped AgNPs were biocompatible with good anti-inflammatory
properties. The blend of antimicrobial and biocompatible properties, coupled with
their intrinsic “green” and facile synthesis, made these biogenic nanoparticles
particularly attractive for future applications in nanomedicine. © The Royal
Society of Chemistry
AU - Zaki, A.
AU - Aziz, M. N.
AU - Ahmad, R.
AU - Ahamad, I.
AU - Ali, M. S.
AU - Yasin, D.
AU - Afzal, B.
AU - Ali, S. M.
AU - Chopra, A.
AU - Hadda, V.
AU - Srivastava, P.
AU - Kumar, R.
AU - Fatma, T.
DB - Scopus
DO - 10.1039/d1ra08396a
IS - 4
KW - Cell culture
Cost effectiveness
Free radicals
Metal nanoparticles
Microorganisms
Particle size
Silver compounds
Cell extracts
Cost effective
Cyanobacterium
Eco-friendly
EDX analysis
Further development
Maximum absorbance
Optimal conditions
Reaction temperature
UV-vis spectrophotometry
Biocompatibility
M3 - Article
PY - 2022
SP - 2497-2510
ST - Synthesis, purification and characterization of
T2 - RSC Advances
TI - Synthesis, purification and characterization of
85123920658&doi=10.1039%2fd1ra08396a&partnerID=40&md5=5ac27257cd919026c3ed931356ede
066
VL - 12
ID - 5064
ER -
TY - JOUR
AB - Driven by the need to biosynthesize alternate biomedical agents to prevent
and treat infection, silver nanoparticles have surfaced as a promising avenue.
Cyanobacteria-derived nanomaterial synthesis is of substantive interest as it
offers an eco-friendly, cost-effective, sustainable, and biocompatible route for
further development. In the present study optimal conditions for synthesis of
silver nanoparticles (AgNPs) were 1 : 9 v/v [cell extract: AgNO3 (1 mM)], pH 7.4,
and 30 degrees C reaction temperatures. Synthesis of nanoparticles was monitored by
UV-vis spectrophotometry and the maximum absorbance was observed at a wavelength of
420 nm. SEM with EDX analysis confirmed 96.85% silver by weight which revealed the
purity of AgNPs. TEM & XRD analysis exhibited a particle size of similar to 12 nm
with crystalline nature. FTIR analysis confirmed the presence of possible
biomolecules involved in the synthesis and stabilization of AgNPs. Decapping of
AgNPs followed by SDS-PAGE, LCMS and MALDI TOF analysis elucidates the
proteinaceous nature of the capping and stabilizing agent. Cyanobacterial-derived
capped AgNPs showed more cytotoxicicity towards a non-small cell lung cancer (A549)
cell line, free radical scavenger and an antimicrobial than de-capped AgNPs. In
addition they showed significant synergistic characteristics with antibiotics and
fungicides. The test revealed that the capped AgNPs were biocompatible with good
anti-inflammatory properties. The blend of antimicrobial and biocompatible
properties, coupled with their intrinsic "green" and facile synthesis, made these
biogenic nanoparticles particularly attractive for future applications in
nanomedicine.
AN - WOS:000743627300001
AU - Zaki, A.
AU - Aziz, M. N.
AU - Ahmad, R.
AU - Ahamad, I.
AU - Ali, M. S.
AU - Yasin, D.
AU - Afzal, B.
AU - Ali, S. M.
AU - Chopra, A.
AU - Hadda, V.
AU - Srivastava, P.
AU - Kumar, R.
AU - Fatma, T.
DA - JAN 12
DO - 10.1039/d1ra08396a
IS - 4
PY - 2022
SN - 2046-2069
SP - 2497-2510
ST - Synthesis, purification and characterization of Plectonema derived AgNPs with
elucidation of the role of protein in nanoparticle stabilization
T2 - RSC ADVANCES
TI - Synthesis, purification and characterization of Plectonema derived AgNPs with
elucidation of the role of protein in nanoparticle stabilization
VL - 12
ID - 6364
ER -
TY - JOUR
AB - Nanosized particles including nanovaccines are a novel approach to the
development of vaccines to combat diseases. Nanovaccines have the promise to
utilize the immune system to cure infections and to prevent infections and diseases
from spreading. Rational vaccine development requires an understanding of vaccine
mediated stimulation of the immune system. We review here immunostimulatory
properties of nanovaccines including their immunogenicity, adjuvant properties,
inflammatory responses and the mechanisms of uptake and stimulation of immune
cells. Examples of various nanoparticles currently being developed as vaccines are
also provided. © 2013 Elsevier Inc.
AU - Zaman, M.
AU - Good, M. F.
AU - Toth, I.
DB - Scopus
DO - 10.1016/j.ymeth.2013.04.014
IS - 3
KW - Adjuvant
Antigen uptake
Delivery system
Immune system
Nanoparticles
Vaccines
Adjuvants, Immunologic
Antigens
Bacterial Vaccines
Dendrimers
Fullerenes
Humans
Immune System
Liposomes
Metal Nanoparticles
Nanotubes
Viral Vaccines
chitosan
gold nanoparticle
macrogol
nanomaterial
nanovaccine
quantum dot
silver nanoparticle
unclassified drug
vaccine
antibody titer
antigen recognition
antigenicity
article
biocompatibility
cell stimulation
cell structure
cellular immunity
deacetylation
dendritic cell
endocytosis
gene delivery system
human
humoral immunity
hydrophobicity
immunization
immunogenicity
immunostimulation
innate immunity
internalization
nanotechnology
nonhuman
oxidative stress
particle size
physical chemistry
priority journal
surface charge
M3 - Article
PY - 2013
SP - 226-231
ST - Nanovaccines and their mode of action
T2 - Methods
TI - Nanovaccines and their mode of action
84878821247&doi=10.1016%2fj.ymeth.2013.04.014&partnerID=40&md5=9e0833f78e622fb02eb5
e0f7b26825d5
VL - 60
ID - 5725
ER -
TY - JOUR
AB - Intrauterine adhesion (IUA) is a common gynecological disease caused by
endometrial injury, which might result in abnormal menstruation, miscarriage, and
even fetal deaths. Nevertheless, existing treatment strategies such as intrauterine
device and uterine cavity balloons only provide a physical barrier, and not
circumvent inflammation of endometrial microenvironment and retrograde infection.
In this study, a slow-controlled bifunctional nano-structure was developed via
encapsulating hyaluronic acid (HA) on surface of silver-metal organic framework
(Ag-MOF), and then loaded in poly lactic-co-glycolic acid scaffold to prevent IUA.
In therapy, macro-molecule of HA provided anti-inflammatory function by the
adjustment of signal transduction pathways of macrophage surface receptors, whereas
Ag-MOF inactivated bacteria by destroying bacterial membrane and producing reactive
oxygen. Significantly, the coated HA effectively avoided burst release of Ag+, thus
achieving long-term antibacterial property and good biocompatibility. Antibacterial
results showed antibacterial rate of the scaffold reached 87.8 % against
staphylococcus aureus. Anti-inflammatory assays showed that the scaffold inhibited
the release of inflammatory cytokines and promoted the release of anti-inflammatory
cytokines. Moreover, in vitro cell tests revealed that the scaffold effectively
inhibited fibroblast growth, indicating its good ability to prevent IUA. Taken
together, the scaffold may be a promising candidate for IUA treatment.
AN - WOS:000927301300001
AU - Zan, J.
AU - Shuai, Y.
AU - Zhang, J.
AU - Zhao, J. C.
AU - Sun, B. X.
AU - Yang, L. Y. M.
C6 - JAN 2023
C7 - 123361
DA - MAR 1
DO - 10.1016/j.ijbiomac.2023.123361
PY - 2023
SN - 0141-8130
1879-0003
ST - Hyaluronic acid encapsulated silver metal organic framework for the
construction of a slow-controlled bifunctional nanostructure: Antibacterial and
anti-inflammatory in intrauterine adhesion repair
TI - Hyaluronic acid encapsulated silver metal organic framework for the
VL - 230
ID - 5991
ER -
TY - JOUR
AB - Intrauterine adhesion (IUA) is a common gynecological disease caused by
endometrial injury, which might result in abnormal menstruation, miscarriage, and
even fetal deaths. Nevertheless, existing treatment strategies such as intrauterine
device and uterine cavity balloons only provide a physical barrier, and not
circumvent inflammation of endometrial microenvironment and retrograde infection.
In this study, a slow-controlled bifunctional nanostructure was developed via
encapsulating hyaluronic acid (HA) on surface of silver-metal organic framework
(Ag-MOF), and then loaded in poly lactic-co-glycolic acid scaffold to prevent IUA.
In therapy, macro-molecule of HA provided anti-inflammatory function by the
adjustment of signal transduction pathways of macrophage surface receptors, whereas
Ag-MOF inactivated bacteria by destroying bacterial membrane and producing reactive
oxygen. Significantly, the coated HA effectively avoided burst release of Ag+, thus
achieving long-term antibacterial property and good biocompatibility. Antibacterial
results showed antibacterial rate of the scaffold reached 87.8 % against
staphylococcus aureus. Anti-inflammatory assays showed that the scaffold inhibited
the release of inflammatory cytokines and promoted the release of anti-inflammatory
cytokines. Moreover, in vitro cell tests revealed that the scaffold effectively
inhibited fibroblast growth, indicating its good ability to prevent IUA. Taken
together, the scaffold may be a promising candidate for IUA treatment. © 2023
Elsevier B.V.
AU - Zan, J.
AU - Shuai, Y.
AU - Zhang, J.
AU - Zhao, J.
AU - Sun, B.
AU - Yang, L.
C7 - 123361
DB - Scopus
DO - 10.1016/j.ijbiomac.2023.123361
Antibacterial
Hyaluronic acid
Slow-controlled release
Cytokines
Female
Humans
Hyaluronic Acid
Metal-Organic Frameworks
Nanostructures
Silver
4',6 diamidino 2 phenylindole
calcein
cell surface receptor
cytokine
dichlorodihydrofluorescein
hyaluronic acid
methanol
nanomaterial
nanoparticle
polyglactin
propidium iodide
silver
silver nitrate
antiinfective agent
animal cell
apoptosis
Article
bacterial membrane
behavior
biocompatibility
cell counting
cell culture
cell proliferation
cell structure
centrifugation
contact angle
controlled study
Escherichia coli
human
in vitro study
inflammation
intrauterine adhesion repair
macromolecule
nonhuman
optical density
RAW 264.7 cell line
skin fibroblast
turbidimetry
uterus synechia
X ray diffraction
chemistry
female
M3 - Article
PY - 2023
ST - Hyaluronic acid encapsulated silver metal organic framework for the
TI - Hyaluronic acid encapsulated silver metal organic framework for the
85147828645&doi=10.1016%2fj.ijbiomac.2023.123361&partnerID=40&md5=a3dbfe3ee13514f05
3b7cc1bef784cff
VL - 230
ID - 5039
ER -
TY - JOUR
AB - Introduction: Biocompatibility and antimicrobial activity of endodontic
materials are of utmost importance. Considering the extensive applications of
mineral trioxide aggregate (MTA) in dentistry and antimicrobial properties of
silver nanoparticles, this study aimed to evaluate the subcutaneous inflammatory
reaction of rat connective tissues to white MTA with and without nanosilver (NS)
particles. Methods and Materials: Polyethylene tubes (1.1×8 mm) containing
experimental materials (MTA and MTA+NS and empty control tubes) were implanted in
subcutaneous tissues of seventy-five male rats. Animals were divided into five
groups (n=15) according to the time of evaluation: group 1; after 7 days, group 2;
after 15 days, group 3; after 30 days, group 4; after 60 days and group 5; after 90
days. The inflammatory reaction was graded and data was analyzed using the Kruskal-
Wallis and Mann- Whitney U tests. Statistical significance was defined at 0.05.
Results: Comparison of cumulative inflammatory reaction at all intervals revealed
that the mean grade of inflammatory reaction to MTA, MTA+NS and control samples
were 3, 2 and 2, respectively. According to the Mann-Whitney analysis there were no
significant differences between MTA+NS and MTA (P=0.42). Conclusion: Incorporation
of 1% nanosilver to MTA does not affect the inflammatory reaction of subcutaneous
tissue in rat models. © 2015, Iranian Association of Endodontics. All rights
Reserved.
AU - Zand, V.
AU - Lotfi, M.
AU - Aghbali, A.
AU - Mesgariabbasi, M.
AU - Janani, M.
AU - Mokhtari, H.
AU - Tehranchi, P.
AU - Pakdel, S. M. V.
DB - Scopus
DO - 10.7508/iej.2016.01.003
IS - 1
Mineral Trioxide Aggregate
Nanosilver
Silver Nanoparticle
M3 - Article
PY - 2016
SP - 13-16
ST - Tissue reaction and biocompatibility of implanted mineral trioxide aggregate
with silver nanoparticles in a rat model
T2 - Iranian Endodontic Journal
TI - Tissue reaction and biocompatibility of implanted mineral trioxide aggregate
with silver nanoparticles in a rat model
84954287127&doi=10.7508%2fiej.2016.01.003&partnerID=40&md5=206e3c0fc7ed1d53268f349b
57e13045
VL - 11
ID - 5549
ER -
TY - JOUR
AB - There is an increasing commercial demand for nanoparticles due to their wide
applicability in various areas such as electronics, catalysis, chemistry, energy,
and medicine. Recently, researchers have tried to synthesize the chemotherapeutic
drugs from metallic nanoparticles especially gold and silver nanoparticles. In the
current study, silver nanoparticles using Spinacia oleracea L. leaf aqueous extract
(AgNPs) are reported for the first time to exert a dietary remedial property
compared to doxorubicin in an animal model of acute myeloid leukemia. The
synthesized AgNPs were characterized using different techniques including UV-Vis.,
EDS, TEM, FT-IR, and FE-SEM. UV-Vis. indicates an absorption band at 462 nm that is
related to the surface plasmon resonance of AgNPs. In EDS, metallic silver
nanocrystals indicated an optical absorption peak at roughly 4keV. TEM and FE-SEM
images exhibited a uniform spherical morphology and diameters of 20–40 nm for the
nanoparticles. FT-IR findings suggested antioxidant compounds in the nanoparticles
were the sources of reducing power, reducing silver ions to AgNPs. In vivo design,
induction of acute myeloid leukemia was done by 7,12-Dimethylbenz[a]anthracene in
75 mice. Then, the animals were randomly divided into six subgroups, including
control, untreated, AgNO3, S. oleracea, AgNPs, and doxorubicin. Similar to
doxorubicin, AgNPs significantly (p ≤ 0.01) reduced the pro-inflammatory cytokines,
and the total WBC, blast, neutrophil, monocyte, eosinophil, and basophil counts and
increased the weight of the body, the anti-inflammatory cytokines and the
lymphocyte, platelet, and RBC parameters as compared to the untreated mice. DPPH
free radical scavenging test was done to evaluate the antioxidant potentials of
AgNO3, S. oleracea, AgNPs, and doxorubicin. DPPH test revealed similar antioxidant
potentials for doxorubicin and AgNPs. For the analyzing of cytotoxicity effects of
AgNO3, S. oleracea, AgNPs, and doxorubicin, MTT assay was used on HUVEC, Human HL-
60/vcr, 32D-FLT3-ITD, and Murine C1498 cell lines. AgNPs similar to doxorubicin had
low cell viability dose-dependently against Human HL-60/vcr, 32D-FLT3-ITD, and
Murine C1498 cell lines without any cytotoxicity on HUVEC cell line. These results
reveal that the inclusion of S. oleracea leaf aqueous extract improves the remedial
effects of AgNPs, which led to a significant enhancement in the antioxidant,
cytotoxicity, and anti-acute myeloid leukemia potentials of the nanoparticles. It
seems that AgNPs can be applied as a chemotherapeutic supplement or drug for the
treatment of acute myeloid leukemia in the clinical trial. © 2019 John Wiley &
Sons, Ltd.
C7 - e5295
DB - Scopus
DO - 10.1002/aoc.5295
IS - 1
Doxorubicin
leukemic mouse model
Spinacia oleracea L.
Antioxidants
Cell culture
Cells
Chemical analysis
Cytotoxicity
Diseases
Free radicals
Light absorption
Mammals
Metal ions
Metal nanoparticles
Morphology
Silver compounds
Mouse models
Spinacia oleracea
Drug delivery
M3 - Article
PY - 2020
ST - Green synthesis and formulation a modern chemotherapeutic drug of Spinacia
oleracea L. leaf aqueous extract conjugated silver nanoparticles; Chemical
characterization and analysis of their cytotoxicity, antioxidant, and anti-acute
myeloid leukemia properties in comparison to doxorubicin in a leukemic mouse model
TI - Green synthesis and formulation a modern chemotherapeutic drug of Spinacia
oleracea L. leaf aqueous extract conjugated silver nanoparticles; Chemical
characterization and analysis of their cytotoxicity, antioxidant, and anti-acute
myeloid leukemia properties in comparison to doxorubicin in a leukemic mouse model
85074818317&doi=10.1002%2faoc.5295&partnerID=40&md5=e224f33dcdfef3e8067fbfcfa187480
c
VL - 34
ID - 5428
ER -
TY - JOUR
AB - This study aimed to investigate the cytotoxicity and pro-inflammatory
responses induced by tungsten disulphide (WS2) and molybdenum disulphide (MoS2)
nanoparticles (NPs) in human bronchial cells (BEAS-2B). For cytotoxicity
assessment, the cells were exposed to different concentrations (2.5-200 mu g/mL) of
WS2-NPs or MoS2-NPs for 24 and 48 h and then the MTT assay was performed.
Afterwards, long-term toxicity was assessed by the colony forming efficiency assay
(CFEA) during a 10 days' exposure of the cells. For pro-inflammatory responses, the
expression of interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) mRNA was
estimated by the real-time PCR method. Both nanomaterials showed similar cytotoxic
effects on BEAS-2B cells assessed by the MTT assay, i.e. reduction in cell
viability to approx. 60-70% at concentrations of 2.5 and 5 mu g/mL after 24 and 48
h. The percentage viability remained relatively constant at this level across all
concentrations above 5 mu g/mL. In long-term exposure, both nanomaterials inhibited
colony formation in a wide range of concentrations up to 100 mu g/mL. MoS2-NPs were
slightly more cytotoxic than WS2-NPs. Additionally, MoS2-NPs caused an increase in
mRNA levels of cytokines, IL-1 beta, and IL-6 at concentration of 50 mu g/mL, while
WS2-NPs did not cause any changes in the level of mRNA for both cytokines. We also
visualised the changes in the cells as a result of WS2-NPs or MoS2-NPs exposure
(2.5 and 25 mu g/mL) via holotomographic microscopy. This work demonstrates the
hazardous potential of both nanomaterials and indicate that WS2 and MoS2
nanoparticles should be included in the occupational risk assessment.
AN - WOS:000769062800001
AU - Zapor, L.
AU - Chojnacka-Puchta, L.
AU - Sawicka, D.
AU - Miranowicz-Dzierzawska, K.
AU - Skowron, J.
DA - MAR 15
DO - 10.1515/ntrev-2022-0073
IS - 1
PY - 2022
SN - 2191-9089
2191-9097
SP - 1263-1272
ST - Cytotoxic and pro-inflammatory effects of molybdenum and tungsten disulphide
on human bronchial cells
T2 - NANOTECHNOLOGY REVIEWS
TI - Cytotoxic and pro-inflammatory effects of molybdenum and tungsten disulphide
on human bronchial cells
VL - 11
ID - 6274
ER -
TY - JOUR
AB - Today, wound healing is an important clinical problem that is often affected
by microbial infection and not paying attention to this problem can cause
irreparable damage to people. Biosynthesis of metal nanoparticles (NPs) has created
a huge revolution in the field of nanomedicine due to their non-toxic,
biocompatible, and stable characteristics. For this purpose, in this study,
Petroselinum crispum seed extract (PCS) was applied to preparation of silver
nanoparticles (AgNPs@PCS). The optimization of the silver nanoparticle synthesis
involved adjusting the concentration of the silver salt, as well as the time and
temperature parameters. After identifying the fabricated nanoparticles by FESEM,
XRD, FT-IR, UV–Vis, TEM and EDS, their biological activity (like antibacterial,
antifungal, antioxidant, anticancer, and wound treatment) was determined. The green
synthesis of AgNPs was confirmed by several characteristics, including the surface
plasmon response with a peak around 420 nm, the presence of both regular and
heterogeneous sizes in the sample, and the observed color change from a clear
solution to a brown color. The antimicrobial properties of the biofabricated AgNPs
were investigated against fungal and various bacteria. The anticancer property of
AgNPs@PCS was demonstrated by an IC50 value of 200 µg/ml after a 24-hour exposure
on the MCF-7 cell line. Also, a dose-dependent antioxidant performance of AgNPs was
found against the DPPH free radical. Histopathological evaluations of AgNPs@PCS
ointment illustrated significant decrease in inflammatory cells. The results showed
that vaseline ointment containing AgNPs@PCS prevented inflammation in the wound
area and increased the number of fibroblast cells, which led to accelerated wound
healing. Furthermore, in-vivo investigation showed the higher percentage of wound
closure on days 7 and 14 was than the control group (Vaseline). Interestingly, the
wound was completely closed after 21 days. Thus, we concluded that Petroselinum
crispum-mediated silver nanoparticles show potential biological activity which can
be used as nano-drug in clinical treatment. © 2023 The Author(s)
AU - Zare-Bidaki, M.
AU - Ghasempour, A.
AU - Mohammadparast-Tabas, P.
AU - Ghoreishi, S. M.
AU - Alamzadeh, E.
AU - Javanshir, R.
AU - Le, B. N.
AU - Barakchi, M.
AU - Fattahi, M.
AU - Mortazavi-Derazkola, S.
C7 - 105194
DB - Scopus
DO - 10.1016/j.arabjc.2023.105194
IS - 10
KW - Antibacterial
Anticancer
Antioxidant
Petroselinum crispum
Silver nanoparticle
Wound healing
Antioxidants
Bioactivity
Biocompatibility
Cell culture
Free radicals
Fungi
Metal nanoparticles
Surface plasmons
Antibacterials
Antifungal activities
Antioxidant and anticancer activities
Clinical problems
In-vivo
Seeds extracts
M3 - Article
PY - 2023
ST - Enhanced in vivo wound healing efficacy and excellent antibacterial,
antifungal, antioxidant and anticancer activities via AgNPs@PCS
T2 - Arabian Journal of Chemistry
TI - Enhanced in vivo wound healing efficacy and excellent antibacterial,
antifungal, antioxidant and anticancer activities via AgNPs@PCS
85166626527&doi=10.1016%2fj.arabjc.2023.105194&partnerID=40&md5=a32c5251c0bfd8ed73b
844701cf2f0c7
VL - 16
ID - 5048
ER -
TY - JOUR
AB - Numerous studies show that nonsteroidal anti-inflammatory drugs (NSAIDs) are
effective in chemoprevention or treatment of cancer. Nevertheless, the mechanisms
underlying these antineoplastic effects remain poorly understood. Here, we report
that induction of the cancer-specific proapoptotic cytokine melanoma
differentiation associated gene-7/interleukin-24 (MDA-7/IL-24) by several NSAIDs is
an essential step for induction of apoptosis and G(2)-M growth arrest in cancer
cells in vitro and inhibition of tumor growth in vivo. We also show that MDA-7/IL-
24-dependent up-regulation of growth arrest and DNA damage inducible 45 a (GADD45
alpha) and GADD45 gamma gene expression is sufficient for cancer cell apoptosis via
c-Jun NH2-terminal kinase (JNK) activation and growth arrest induction through
inhibition of Cdc2-cyclin B checkpoint kinase. Knockdown of GADD45 alpha and GADD45
gamma transcription by small interfering RNA abrogates apoptosis and growth arrest
induction by the NSAID treatment, blocks JNK activation, and restores Cdc2-cyclin B
kinase activity. Our results establish MDA-7/IL-24 and GADD45 alpha and GADD45
gamma as critical mediators of apoptosis and growth arrest in response to NSAIDs in
cancer cells.
AN - WOS:000242915600050
AU - Zerbini, L. F.
AU - Czibere, A.
AU - Wang, Y. H.
AU - Correa, R. G.
AU - Otu, H.
AU - Joseph, M.
AU - Takayasu, Y.
AU - Silver, M.
AU - Gu, X. S.
AU - Ruchusatsawat, K.
AU - Li, L. L.
AU - Sarkar, D.
AU - Zhou, J. R.
AU - Fisher, P. R.
AU - Libermann, T. A.
DA - DEC 15
DO - 10.1158/0008-5472.CAN-06-2068
IS - 24
PY - 2006
SN - 0008-5472
1538-7445
SP - 11922-11931
ST - A novel pathway involving melanoma differentiation associated
gene-7/interleukin-24 mediates nonsteroidal anti-inflammatory drug-induced
apoptosis and growth arrest of cancer cells
T2 - CANCER RESEARCH
TI - A novel pathway involving melanoma differentiation associated
gene-7/interleukin-24 mediates nonsteroidal anti-inflammatory drug-induced
apoptosis and growth arrest of cancer cells
VL - 66
ID - 6168
ER -
TY - JOUR
AB - Nanosilver is one of the most commercialized nanomaterials in the world. Due
to its unique surface plasma resonance performance and excellent antibacterial
activities, nanosilver has been widely used in many fields, such as medical area,
health care, industrial products and our daily supplies. Meanwhile, the increasing
application of nanosilver has drawn more and more attention to its biosafety.
Previous toxicological studies have revealed diverse deleterious effects nanosilver
may cause, wherein, neurotoxicity is highly concerned. This review mainly focuses
on the neurotoxicological effects of nanosilver, and three aspects, including the
bioaccumulation of nanosilver in brain and its penetration routes,
neurotoxicological effects and the underlying molecular mechanisms, and the
influencing factors, are comprehensively discussed. The administration of
nanosilver through diverse ways could cause brain silver accumulation, and its
penetration routes to the brain were mainly involved with the direct nasal
olfactory nerve transfer and the translocation of the blood-brain barrier. The
neurotoxicological effects of nanosilver were evidenced by neurobehavioral changes
in the exposed animals, histopathological alteration in the brain or cellular
morphological changes in neurons and neuroglia cells, and the disturbance in the
neurontransmitter secretion. The underlying mechanisms were related with oxidative
damage and inflammatory responses. The factors, including particle size, surface
coating and silver ion release, would potentially determine nanosilver induced
neurotoxicity. Finally, the existing problems in neurotoxicological studies on
nanosilver are pointed out, and the future perspectives in this area are proposed.
The review would be of great help to risk assessment of the production, application
and disposal of nanosilver.
AN - WOS:000451112500010
AU - Zhang, B. J.
AU - Liu, Q. S.
AU - Zhou, Q. F.
AU - Zhang, J. Q.
AU - Jiang, G. B.
DA - SEP 15
DO - 10.7536/PC171228
IS - 9
PY - 2018
SN - 1005-281X
SP - 1392-1402
ST - Neurotoxicological Effects of Nanosilver
T2 - PROGRESS IN CHEMISTRY
TI - Neurotoxicological Effects of Nanosilver
VL - 30
ID - 6769
ER -
TY - JOUR
AB - Antibacterial wound dressings with high flexibility and biocompatibility are
attracting increasing attention, especially for applications on flexible body parts
such as joints. Bovine serum albumin (BSA) fibrous films were integrated with
glycerol to acquire suitable mechanical properties. After the decoration of silver,
the as-synthesized G-BSA-Ag films combined the antibacterial properties of silver,
the biocompatibility of BSA and the wettability of glycerol, being a promising
candidate for bacteria-infected wound dressing. The G-BSA-Ag films exhibited good
extensibility (elongation at break > 210% for G-BSA-Ag-22), transmittance (>60% at
600 nm for G-BSA-Ag-22) and appropriate swelling ability, maintaining a moist and
visible healing environment to promote wound healing. The in vitro and in vivo
biocompatibility analysis of G-BSA-Ag films confirmed their non-hemolysis, durable
antibacterial properties, non-cytotoxicity, and promotion to cell migration.
Furthermore, the G-BSA-Ag films were applied on a S. aureus-infected mouse wound
model, showing accelerated healing rate with reduced inflammatory factor levels,
proving their good therapeutic effects on wound healing, and demonstrating good
potential in clinical applications.
AN - WOS:000653229500180
AU - Zhang, F.
AU - Yang, H.
AU - Yang, Y. Q.
AU - Wang, H.
AU - Li, X. F.
AU - Wu, X. C.
C6 - MAR 2021
C7 - 129145
DA - AUG 1
DO - 10.1016/j.cej.2021.129145
PY - 2021
SN - 1385-8947
1873-3212
ST - Stretchable and biocompatible bovine serum albumin fibrous films supported
silver for accelerated bacteria-infected wound healing
TI - Stretchable and biocompatible bovine serum albumin fibrous films supported
VL - 417
ID - 6305
ER -
TY - JOUR
AB - Antibacterial wound dressings with high flexibility and biocompatibility are
attracting increasing attention, especially for applications on flexible body parts
such as joints. Bovine serum albumin (BSA) fibrous films were integrated with
glycerol to acquire suitable mechanical properties. After the decoration of silver,
the as-synthesized G-BSA-Ag films combined the antibacterial properties of silver,
the biocompatibility of BSA and the wettability of glycerol, being a promising
candidate for bacteria-infected wound dressing. The G-BSA-Ag films exhibited good
extensibility (elongation at break > 210% for G-BSA-Ag22), transmittance (>60% at
600 nm for G-BSA-Ag22) and appropriate swelling ability, maintaining a moist and
visible healing environment to promote wound healing. The in vitro and in vivo
biocompatibility analysis of G-BSA-Ag films confirmed their non-hemolysis, durable
antibacterial properties, non-cytotoxicity, and promotion to cell migration.
Furthermore, the G-BSA-Ag films were applied on a S. aureus-infected mouse wound
model, showing accelerated healing rate with reduced inflammatory factor levels,
proving their good therapeutic effects on wound healing, and demonstrating good
potential in clinical applications. © 2021 Elsevier B.V.
AU - Zhang, F.
AU - Yang, H.
AU - Yang, Y.
AU - Wang, H.
AU - Li, X.
AU - Wu, X.
C7 - 129145
DB - Scopus
DO - 10.1016/j.cej.2021.129145
KW - Antibacterial
Bacteria-infected wound healing
Bovine serum albumin
Fibrous film
Silver
Bacteria
Biocompatibility
Glycerol
Mammals
Ag films
Ag$++$
Antibacterials
Bovine serum albumins
Infected wounds
Wound dressings
Wound healing
M3 - Article
PY - 2021
ST - Stretchable and biocompatible bovine serum albumin fibrous films supported
T2 - Chemical Engineering Journal
TI - Stretchable and biocompatible bovine serum albumin fibrous films supported
85103017410&doi=10.1016%2fj.cej.2021.129145&partnerID=40&md5=fe71226cd514ccf5ff83fb
820a93950b
VL - 417
ID - 5247
ER -
TY - JOUR
AB - Antibacterial hydrogels are intensively studied as wound dressings. Silk
fibroin (SF) is chemical crosslinked to glycyrrhizic acid (GA) and silver to
fabricate a hydrogel dressing with both antibacterial and anti-inflammatory
properties. The SF/Ag/GA hydrogel exhibits high water content with acceptable
mechanical properties, combines the good biocompatibility and biodegradability of
SF, the antibacterial activity of silver, and the anti-inflammatory property of GA,
capable to promote tissue regeneration during wound healing process, offering great
potential as an alternative for wound dressings.
AN - WOS:000743095400001
AU - Zhang, F.
AU - Yin, C. J.
AU - Qi, X. J.
AU - Guo, C. L.
AU - Wu, X. C.
C6 - JAN 2022
C7 - 2100407
DA - APR
DO - 10.1002/mabi.202100407
IS - 4
PY - 2022
SN - 1616-5187
1616-5195
ST - Silk Fibroin Crosslinked Glycyrrhizic Acid and Silver Hydrogels for
Accelerated Bacteria-Infected Wound Healing
T2 - MACROMOLECULAR BIOSCIENCE
TI - Silk Fibroin Crosslinked Glycyrrhizic Acid and Silver Hydrogels for
VL - 22
ID - 6184
ER -
TY - JOUR
AB - Antibacterial hydrogels are intensively studied as wound dressings. Silk
fibroin (SF) is chemical crosslinked to glycyrrhizic acid (GA) and silver to
fabricate a hydrogel dressing with both antibacterial and anti-inflammatory
properties. The SF/Ag/GA hydrogel exhibits high water content with acceptable
mechanical properties, combines the good biocompatibility and biodegradability of
SF, the antibacterial activity of silver, and the anti-inflammatory property of GA,
capable to promote tissue regeneration during wound healing process, offering great
potential as an alternative for wound dressings. © 2022 Wiley-VCH GmbH.
AU - Zhang, F.
AU - Yin, C.
AU - Qi, X.
AU - Guo, C.
AU - Wu, X.
C7 - 2100407
DB - Scopus
DO - 10.1002/mabi.202100407
IS - 4
KW - glycyrrhizic acid
silk fibroin
silver
wound dressings
Bacteria
Fibroins
Glycyrrhizic Acid
Humans
Hydrogels
Silk
Silver
Wound Healing
Wound Infection
Biocompatibility
Biodegradability
Biomechanics
Crosslinking
Tissue regeneration
chloral hydrate
hydrogel
water
antiinfective agent
fibroin
silk
Anti-inflammatories
Antibacterials
Crosslinked
Glycyrrhizic acid
Hydrogels dressings
Infected wounds
Inflammatory properties
Silk fibroin
Wound dressings
Wound healing
adsorption
animal experiment
animal model
animal tissue
Article
bacterial infection
bacterium colony
biocompatibility
biodegradability
chorioallantoic membrane assay
controlled study
cross linking
histopathology
in vitro study
male
mouse
nonhuman
skin injury
tissue regeneration
water content
wound healing
wound healing assay
wound infection
zone of inhibition
bacterium
chemistry
human
pharmacology
M3 - Article
PY - 2022
ST - Silk Fibroin Crosslinked Glycyrrhizic Acid and Silver Hydrogels for
TI - Silk Fibroin Crosslinked Glycyrrhizic Acid and Silver Hydrogels for
85122722846&doi=10.1002%2fmabi.202100407&partnerID=40&md5=8907b8dbd6db6f6d4f2239f7d
10c4377
VL - 22
ID - 5070
ER -
TY - JOUR
AB - Objective The biosafety and efficacy of
silver-hydroxyapatite-titania/polyamide nanocomposite (nAg-HA-TiO2/PA) membrane as
a guided bone regeneration (GBR) barrier were investigated based on a rat
subcutaneous and critical-size calvarial defect model. Material and methods Thirty-
six Sprague-Dawley albino rats were divided into nAg-HA-TiO2/PA membrane test,
expanded polytetrafluoroethylene (e-PTFE) membrane control and blank control.
Inflammatory response and bone regeneration in each group were evaluated using
morphological, serological, radiographic and histological techniques at 1, 4 and 8
weeks, respectively, after implantation. Results For subcutaneous implantation,
slight degradation of nAg-HA-TiO2/PA membranes was observed by scanning electron
microscope at 4 and 8 weeks. Histopathologic examination demonstrated a thinner
layer of granulation tissue in the vicinity of nAg-HA-TiO2/PA membranes than that
of e-PTFE membranes. For cranial defect implantation, the serum alkaline
phosphatase level was remarkably higher in nAg-HA-TiO2/PA group than that in e-PTFE
group. Radiographic and histomorphometric analysis showed a fully closed cranial
defect for both nAg-HA-TiO2/PA and e-PTFE groups at 8 weeks. No remarkable
difference was found between the two groups regarding the integral optical density
of neo-bone at each time interval. Conclusion nAg-HA-TiO2/PA membranes demonstrated
better biocompatibility and similar osteoinductive activity compared with e-PTFE
membranes. nAg-HA-TiO2/PA composite membranes provided a good prospect for further
research and development in anti-bacterial GBR membrane. To cite this article:Zhang
JC, Xu Q, Huang C, Mo AC, Li JD, Zuo Y. Biological properties of an anti-bacterial
membrane for guided bone regeneration: an experimental study in rats.Clin. Oral
Impl. Res. 21, 2010; 321-327.doi: 10.1111/j.1600-0501.2009.01838.x.
AN - WOS:000274820800011
AU - Zhang, J. C.
AU - Xu, Q.
AU - Huang, C.
AU - Mo, A. C.
AU - Li, J. D.
AU - Zuo, Y.
DA - MAR
DO - 10.1111/j.1600-0501.2009.01838.x
IS - 3
PY - 2010
SN - 0905-7161
1600-0501
SP - 321-327
ST - Biological properties of an anti-bacterial membrane for guided bone
regeneration: an experimental study in rats
T2 - CLINICAL ORAL IMPLANTS RESEARCH
TI - Biological properties of an anti-bacterial membrane for guided bone
regeneration: an experimental study in rats
VL - 21
ID - 6747
ER -
TY - JOUR
AB - In the recent study, we decided to survey the capacities of metallic
nanoparticles formulated by Allium monanthum (AgNPs) as a novel chemotherapeutic
drug in the treatment of several types of breast cancers. Characterization of AgNPs
was done by UV-Visible Spectroscopy (UV- Vis), Fourier Transformed Infrared
Spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), and Field Emission
Scanning Electron Microscopy (FE-SEM). For investigating the antioxidant properties
of AgNO3, Allium monanthum, and AgNPs, the DPPH test was used in the presence of
butylated hydroxytoluene as the positive control. To survey the cytotoxicity and
anti-breast cancer effects of AgNO3, Allium monanthum, and AgNPs, MTT assay was
used on the breast adenocarcinoma (MCF7), breast carcinoma (Hs 578Bst),
infiltrating ductal cell carcinoma (Hs 319.T), infiltrating lobular carcinoma of
breast (UACC-3133), inflammatory carcinoma of the breast (UACC-732), and metastatic
carcinoma (MDA-MB-453) cell lines. DPPH test revealed similar antioxidant
potentials for Allium monanthum, AgNPs, and butylated hydroxytoluene. Silver
nanoparticles had very low cell viability and anti-breast cancer properties dose
dependently against MCF7, Hs 578Bst, Hs 319.T, UACC-3133, UACC-732, and MDA-MB-453
cell lines without any cytotoxicity on the normal cell line. The best result of
anti-breast cancer properties of AgNPs against the above cell lines was seen in the
case of the UACC-3133 cell line. According to the above findings, the silver
nanoparticles containing Allium monanthum aqueous extract can be administrated in
humans for the treatment of several types of breast cancer especially breast
adenocarcinoma, breast carcinoma, infiltrating ductal cell carcinoma, infiltrating
lobular carcinoma of breast, inflammatory carcinoma of the breast, and metastatic
carcinoma.& nbsp;(c) 2022 Published by Elsevier B.V. on behalf of King Saud
University. This is an open access article under the CC BY-NC-ND license
AN - WOS:000777772300010
AU - Zhang, J. K.
AU - Sun, J. R.
AU - Geng, H. J.
AU - Ma, Q. S.
AU - Li, C.
C6 - FEB 2022
C7 - 103693
DA - APR
DO - 10.1016/j.arabjc.2022.103693
IS - 4
PY - 2022
SN - 1878-5352
1878-5379
ST - Application of organometallic chemistry in the formulation of a modern
therapeutic drug by Ag nanoparticles green-mediated by Allium to treat the breast
cancer
TI - Application of organometallic chemistry in the formulation of a modern
cancer
VL - 15
ID - 6173
ER -
TY - JOUR
AB - The biological properties of the lanthanides, primarily based on their
similarity to calcium, have been the basis for research into potential therapeutic
applications of lanthanides since the early part of the twentieth century. Up to
date, cerium nitrate has been used as a topical cream with silver sulfadiazene for
the treatment of burn wounds. A lanthanide texaphyrin complex (motexafin
gadolinium) has been evaluated through Phase III clinical trials for the treatment
of brain metastases in non-small cell lung cancer. Lanthanum carbonate (Fosrenol)
as a phosphate binder has been approved for the treatment of hyperphosphatemia in
renal dialysis patients in both the USA and Europe. This review will highlight
therapeutic applications of the lanthanides for burn wounds, cancer,
hyperphosphatemia, immune function, magnetic resonance imaging (MRI) contrast
agents and osteoporosis, and discuss their future potential in the medical fields.
© 2011 Bentham Science Publishers Ltd.
AU - Zhang, J.
AU - Li, Y.
AU - Hao, X.
AU - Zhang, Q.
AU - Yang, K.
AU - Li, L.
AU - Ma, L.
AU - Wang, S.
AU - Li, X.
DB - Scopus
DO - 10.2174/138955711796268804
IS - 8
KW - Burn wounds
Cancer
Hyperphosphatemia
Immune function
Lanthanides
Mri contrast agents
Osteoporosis
Therapeutic application
acetic acid
antibiotic agent
antiinfective agent
cerium
cerium iodide
cerium nitrate
chloride
contrast medium
dermacerium
flammacerium
gadobenate dimeglumine
gadobutrol
gadodiamide
gadofosveset
gadolinium pentetate
gadolinium texaphyrin
gadoterate meglumine
gadoteridol
gadoversetamide
gadoxetic acid
lanthanide
lanthanum carbonate
lanthanum complex
metal complex
metal derivative
neodymium complex
nitrate
stearic acid
sulfadiazine silver
unclassified drug
unindexed drug
warfarin
biocompatibility
brain metastasis
burn
cancer survival
chelation
clinical trial (topic)
complex formation
cytotoxicity
diagnostic imaging
drug efficacy
drug mechanism
drug safety
drug synthesis
drug tolerability
food and drug administration
human
hyperphosphatemia
immune system
immunomodulation
in vitro study
in vivo study
liver protection
lung non small cell cancer
mortality
multiple organ failure
neoplasm
nonhuman
osteoporosis
radioimmunotherapy
review
systemic inflammatory response syndrome
M3 - Review
PY - 2011
SP - 678-694
ST - Recent progress in therapeutic and diagnostic applications of lanthanides
T2 - Mini-Reviews in Medicinal Chemistry
TI - Recent progress in therapeutic and diagnostic applications of lanthanides
79960443580&doi=10.2174%2f138955711796268804&partnerID=40&md5=4276033351197697dce3d
883bb77d00b
VL - 11
ID - 5731
ER -
TY - JOUR
AB - In the recent study, we decided to survey the capacities of metallic
nanoparticles formulated by Allium monanthum (AgNPs) as a novel chemotherapeutic
drug in the treatment of several types of breast cancers. Characterization of AgNPs
was done by UV–Visible Spectroscopy (UV–Vis), Fourier Transformed Infrared
Spectroscopy (FT‐IR), Transmission Electron Microscopy (TEM), and Field Emission
Scanning Electron Microscopy (FE‐SEM). For investigating the antioxidant properties
of AgNO3, Allium monanthum, and AgNPs, the DPPH test was used in the presence of
butylated hydroxytoluene as the positive control. To survey the cytotoxicity and
anti-breast cancer effects of AgNO3, Allium monanthum, and AgNPs, MTT assay was
used on the breast adenocarcinoma (MCF7), breast carcinoma (Hs 578Bst),
infiltrating ductal cell carcinoma (Hs 319.T), infiltrating lobular carcinoma of
breast (UACC-3133), inflammatory carcinoma of the breast (UACC-732), and metastatic
carcinoma (MDA-MB-453) cell lines. DPPH test revealed similar antioxidant
potentials for Allium monanthum, AgNPs, and butylated hydroxytoluene. Silver
nanoparticles had very low cell viability and anti-breast cancer properties dose-
dependently against MCF7, Hs 578Bst, Hs 319.T, UACC-3133, UACC-732, and MDA-MB-453
cell lines without any cytotoxicity on the normal cell line. The best result of
anti-breast cancer properties of AgNPs against the above cell lines was seen in the
case of the UACC-3133 cell line. According to the above findings, the silver
nanoparticles containing Allium monanthum aqueous extract can be administrated in
humans for the treatment of several types of breast cancer especially breast
adenocarcinoma, breast carcinoma, infiltrating ductal cell carcinoma, infiltrating
lobular carcinoma of breast, inflammatory carcinoma of the breast, and metastatic
carcinoma. © 2022
AU - Zhang, J.
AU - Sun, J.
AU - Geng, H.
AU - Ma, Q.
AU - Li, C.
C7 - 103693
DB - Scopus
DO - 10.1016/j.arabjc.2022.103693
IS - 4
KW - Breast cancer
Chemotherapeutic drug
Antioxidants
Cells
Diseases
Drug delivery
Metal nanoparticles
Silver compounds
Surveys
Breast Cancer
Breast carcinomas
Butylated hydroxytoluene
Cell carcinoma
Cell lines
Metastatic carcinoma
Organometallic chemistry
Property
Therapeutic drugs
Cell culture
M3 - Article
PY - 2022
ST - Application of organometallic chemistry in the formulation of a modern
cancer
T2 - Arabian Journal of Chemistry
TI - Application of organometallic chemistry in the formulation of a modern
cancer
85124027009&doi=10.1016%2fj.arabjc.2022.103693&partnerID=40&md5=328d2c6eb752f7b67b4
eea2278332f97
VL - 15
ID - 5156
ER -
TY - JOUR
AB - Objective: The biosafety and efficacy of
silver-hydroxyapatite-titania/polyamide nanocomposite (nAg-HA-TiO2/PA) membrane as
a guided bone regeneration (GBR) barrier were investigated based on a rat
subcutaneous and critical-size calvarial defect model. Material and methods:
Thirty-six Sprague-Dawley albino rats were divided into nAg-HA-TiO2/PA membrane
test, expanded polytetrafluoroethylene (e-PTFE) membrane control and blank control.
Inflammatory response and bone regeneration in each group were evaluated using
morphological, serological, radiographic and histological techniques at 1, 4 and 8
weeks, respectively, after implantation. Results: For subcutaneous implantation,
slight degradation of nAg-HA-TiO2/PA membranes was observed by scanning electron
microscope at 4 and 8 weeks. Histopathologic examination demonstrated a thinner
layer of granulation tissue in the vicinity of nAg-HA-TiO2/PA membranes than that
of e-PTFE membranes. For cranial defect implantation, the serum alkaline
phosphatase level was remarkably higher in nAg-HA-TiO2/PA group than that in e-PTFE
group. Radiographic and histomorphometric analysis showed a fully closed cranial
defect for both nAg-HA-TiO2/PA and e-PTFE groups at 8 weeks. No remarkable
difference was found between the two groups regarding the integral optical density
of neo-bone at each time interval. Conclusion: nAg-HA-TiO2/PA membranes
demonstrated better biocompatibility and similar osteoinductive activity compared
with e-PTFE membranes. nAg-HA-TiO2/PA composite membranes provided a good prospect
for further research and development in anti-bacterial GBR membrane. © 2010 John
Wiley & Sons A/S.
AU - Zhang, J.
AU - Xu, Q.
AU - Huang, C.
AU - Mo, A.
AU - Li, J.
AU - Zuo, Y.
DB - Scopus
DO - 10.1111/j.1600-0501.2009.01838.x
IS - 3
KW - Anti-bacterial
Guided bone regeneration
Hydroxyapatite
In vivo
Membrane
Polyamide
Animals
Bone Regeneration
Durapatite
Guided Tissue Regeneration, Periodontal
Male
Nanocomposites
Nylons
Random Allocation
Rats
Silver
Skull
Subcutaneous Tissue
Titanium
antiinfective agent
biomaterial
hydroxyapatite
nanocomposite
nylon
politef
silver
titanium
titanium dioxide
animal
article
artificial membrane
bone regeneration
chemistry
evaluation
male
methodology
periodontics
randomization
rat
skull
Sprague Dawley rat
subcutaneous tissue
M3 - Article
PY - 2010
SP - 321-327
ST - Biological properties of an anti-bacterial membrane for guided bone
regeneration: An experimental study in rats
T2 - Clinical Oral Implants Research
TI - Biological properties of an anti-bacterial membrane for guided bone
regeneration: An experimental study in rats
76949106942&doi=10.1111%2fj.1600-
0501.2009.01838.x&partnerID=40&md5=60516d844b0b2691dfaaef47fdfab2c3
VL - 21
ID - 5684
ER -
TY - JOUR
AB - Our aims of the research were to study the antimicrobial effect of
dimethylaminododecyl methacrylate (DMADDM) modified denture base resin on multi-
species biofilms and the biocompatibility of this modified dental material. Candida
albicans (C. albicans), Streptococcus mutans (S. mutans), Streptococcus sanguinis
(S. sanguinis), as well as Actinomyces naeslundii (A. naeslundii) were used for
biofilm formation on denture base resin. Colony forming unit (CFU) counts,
microbial viability staining, and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-
tetrazolium-5-carboxanilide (XTT) array were used to evaluate the antimicrobial
effect of DMADDM. C. albicans staining and Real-time PCR were used to analyze the
morphology and expression of virulence genes of C. albicans in biofilm. Lactate
dehydrogenase (LDH) array and Real-time PCR were conducted to examine the results
after biofilm co-cultured with epithelial cell. Hematoxylin and eosin (HE) staining
followed by histological evaluation were used to study the biocompatibility of this
modified material. We found that DMADDM containing groups reduced both biomass and
metabolic activity of the biofilm significantly. DMADDM can also inhibit the
virulence of C. albicans by means of inhibiting the hyphal development and
downregulation of two virulence related genes. DMADDM significantly reduced the
cell damage caused by multi-species biofilm according to the LDH activity and
reduced the expression of IL-18 gene of the cells simultaneously. The in vivo
histological evaluation proved that the addition of DMADDM less than 6.6% in
denture material did not increase the inflammatory response (p > 0.05). Therefore,
we proposed that the novel denture base resin containing DMADDM may be considered
as a new promising therapeutic system against problems caused by microbes on
denture base such as denture stomatitis.
AN - WOS:000381500900048
AU - Zhang, K. K.
AU - Ren, B.
AU - Zhou, X. D.
AU - Xu, H. H. K.
AU - Chen, Y.
AU - Han, Q.
AU - Li, B. L.
AU - Weir, M. D.
AU - Li, M. Y.
AU - Feng, M. Y.
AU - Cheng, L.
C7 - 1033
DA - JUL
DO - 10.3390/ijms17071033
IS - 7
PY - 2016
SN - 1422-0067
ST - Effect of Antimicrobial Denture Base Resin on Multi-Species Biofilm Formation
TI - Effect of Antimicrobial Denture Base Resin on Multi-Species Biofilm Formation
VL - 17
ID - 6552
ER -
TY - JOUR
AB - Although metal-based agents are widely used in disease treatment, precisely
controlled metal ions release is still a challenge. Here, we demonstrated a
nanoplatform (PAM) to achieve on-demand activation and release of metal ions via
controlling oxidation condition by near infrared (NIR) light-inducted photodynamic
therapy (PDT). PAM was constructed by decorating silver nanoparticles (AgNPs) onto
the porphyrinic porous coordination network (PCN) and further camouflaging with the
neutrophil membrane (NM) with inflammatory targeting ability. PAM was inactive
without irradiation, causing no damage to normal tissues. However, under NIR
irradiation at tumor or infected tissues, PCN locally generated singlet oxygen (O-
1(2)), enabling AgNPs to be partly degraded to release cytotoxic Ag+ for metal ions
therapy (MIT). Simultaneously, the incorporated AgNPs promoted the O-1(2) yield of
PCN due to the localized electric field effect. Consequently, the NIR light-
controlled interlocking interactions between AgNPs and PCN might offer a great
potential for achieving controlled, precise and efficient disease treatment with
reduced side-effect.
AN - WOS:000528266100008
AU - Zhang, L.
AU - Cheng, Q.
AU - Li, C. X.
AU - Zeng, X.
AU - Zhang, X. Z.
C7 - 120029
DA - JUL
PY - 2020
SN - 0142-9612
1878-5905
ST - Near infrared light-triggered metal ion and photodynamic therapy based on
AgNPs/porphyrinic MOFs for tumors and pathogens elimination
T2 - BIOMATERIALS
TI - Near infrared light-triggered metal ion and photodynamic therapy based on
VL - 248
ID - 6484
ER -
TY - JOUR
AB - Although metal-based agents are widely used in disease treatment, precisely
controlled metal ions release is still a challenge. Here, we demonstrated a
nanoplatform (PAM) to achieve on-demand activation and release of metal ions via
controlling oxidation condition by near infrared (NIR) light-inducted photodynamic
therapy (PDT). PAM was constructed by decorating silver nanoparticles (AgNPs) onto
the porphyrinic porous coordination network (PCN) and further camouflaging with the
neutrophil membrane (NM) with inflammatory targeting ability. PAM was inactive
without irradiation, causing no damage to normal tissues. However, under NIR
irradiation at tumor or infected tissues, PCN locally generated singlet oxygen
(1O2), enabling AgNPs to be partly degraded to release cytotoxic Ag+ for metal ions
therapy (MIT). Simultaneously, the incorporated AgNPs promoted the 1O2 yield of PCN
due to the localized electric field effect. Consequently, the NIR light-controlled
interlocking interactions between AgNPs and PCN might offer a great potential for
achieving controlled, precise and efficient disease treatment with reduced side-
effect. © 2020 Elsevier Ltd
AU - Zhang, L.
AU - Cheng, Q.
AU - Li, C.
AU - Zeng, X.
AU - Zhang, X. Z.
C7 - 120029
DB - Scopus
KW - Antibiosis
Antitumor
Inflammation targeting
Metal ions therapy
Humans
Infrared Rays
Ions
Metal Nanoparticles
Neoplasms
Photochemotherapy
Photosensitizing Agents
Silver
Diseases
Electric field effects
Histology
Infrared devices
Irradiation
Metal ions
Metals
Silver compounds
Tumors
metal ion
silver nanoparticle
singlet oxygen
ion
metal nanoparticle
silver
Anti-tumors
Near infrared light
Oxidation conditions
Photodynamic therapy (PDT)
Porous coordination networks
animal cell
animal experiment
animal model
Article
colon adenocarcinoma
controlled study
cytotoxicity
degradation
electric field
female
infrared radiation
mouse
nonhuman
oxidation
photodynamic therapy
priority journal
human
neoplasm
photochemotherapy
M3 - Article
PY - 2020
ST - Near infrared light-triggered metal ion and photodynamic therapy based on
T2 - Biomaterials
TI - Near infrared light-triggered metal ion and photodynamic therapy based on
85082932883&doi=10.1016%2fj.biomaterials.2020.120029&partnerID=40&md5=204c0ebb335d7
748384040d731caa5fb
VL - 248
ID - 5299
ER -
TY - JOUR
AB - In this paper, Ag-Metal−organic framework loaded chitosan nanoparticles
(0.1%Ag@MOF/1.5%CSNPs) and polyvinyl alcohol/sodium alginate/chitosan (PACS) were
used as the upper and lower layers to successfully prepare a bilayer composite
dressing for wound healing. The performance of bilayer dressing was evaluated. The
lower layer (PACS) had uniform pore size distribution, good water retention,
swelling, water vapor permeability, and biocompatibility while PACS had almost no
antibacterial activity. The upper layer (Ag@MOF/CSNPs) possessed excellent
antibacterial activity and poor biocompatibility. As the upper layer, it can avoid
direct contact with the skin and inhibit microbial invasion. In addition, the
bilayer can adhere to a large number of red blood cells and platelets, promoting
blood coagulation and cell proliferation. Ag@MOF, CSNPs, Ag@MOF/CSNPs and bilayer
showed antibacterial activity in ascending order, due to the synergistic
antibacterial action of the upper and lower layer. In vivo evaluation showed that
both bilayer and PACS could significantly accelerate the wound healing, and the
bilayer dressing showed more complete re-epithelialization with less inflammatory
cells. In summary, this new bilayer composite is an ideal dressing for accelerating
wound healing. © 2021 Elsevier B.V.
AU - Zhang, M.
AU - Wang, G.
AU - Wang, D.
AU - Zheng, Y.
AU - Li, Y.
AU - Meng, W.
AU - Zhang, X.
AU - Du, F.
AU - Lee, S.
DB - Scopus
DO - 10.1016/j.ijbiomac.2021.02.045
Bilayer
Wound healing
Alginates
Animals
Bandages
Cell Line
Chitosan
Hydrogels
Male
Metal Nanoparticles
Metal-Organic Frameworks
Mice
Mice, Inbred BALB C
Polyvinyl Alcohol
Silver
Skin
Wound Healing
alginic acid
hydrogel
polyvinyl alcohol
silver nanoparticle
antiinfective agent
chitosan
metal nanoparticle
silver
animal cell
animal experiment
animal model
animal tissue
Article
biocompatibility
biodegradation
blood clotting
cell adhesion
cell proliferation
controlled study
epithelization
erythrocyte count
hemostasis
in vivo study
inflammatory cell
male
microbial contamination
mouse
nonhuman
physical chemistry
platelet count
swelling
water permeability
water retention
water vapor
wound
wound healing
animal
Bagg albino mouse
bandage
cell line
chemistry
drug effect
microbiology
pharmacology
skin
synthesis
M3 - Article
PY - 2021
SP - 481-494
ST - Ag@MOF-loaded chitosan nanoparticle and polyvinyl alcohol/sodium
alginate/chitosan bilayer dressing for wound healing applications
TI - Ag@MOF-loaded chitosan nanoparticle and polyvinyl alcohol/sodium
alginate/chitosan bilayer dressing for wound healing applications
85100794530&doi=10.1016%2fj.ijbiomac.2021.02.045&partnerID=40&md5=fe820c66328117a76
31eeec56982b63c
VL - 175
ID - 5243
ER -
TY - JOUR
AB - The creation of wound dressings with low drug resistance and broad-spectrum
antibacterial capability is a key topic of scientific interest. To achieve this, a
bactericidal wound dressing with the capacity to autocatalytically produce hydroxyl
radicals ([rad]OH) was developed. The wound dressing was an electrospun
PCL/gelatin/glucose composite fiber mesh (PGD) with functional iron-containing
metal-organic framework (Fe-MOF) nanozymes. These functional nanozymes (G@Fe) were
formed by coupling glucose oxidase (GOx) and Fe-MOF through amide bonds. These
nanozymes enabled the conversion of glucose released from the PGD composite mesh
into hydroxyl radicals via an autocatalytic cascade reaction to destroy bacteria.
The antibacterial efficiency of wound dressings and their stimulation of tissue
regeneration were assessed using a MRSA-infected skin wound infection model on the
back of SD mice. The G@Fe/PGD wound dressing exhibited improved wound healing
capacity and had comparable biosafety to commercial silver-containing dressings,
suggesting a potential replacement in the future. © 2023
AU - Zhang, P.
AU - Xu, X.
AU - He, W.
AU - Li, H.
AU - Huang, Y.
AU - Wu, G.
C7 - 102683
DB - Scopus
DO - 10.1016/j.nano.2023.102683
KW - Antibacterial
Autocatalytic
Hydroxyl radical
Metal-organic framework nanozyme
Wound dressing
Animals
Bacteria
Bandages
Glucose
Mice
Wound Healing
Wound Infection
Amides
Glucose oxidase
Glucose sensors
Hydrogen bonds
Iron compounds
Mammals
Mesh generation
Organometallics
Tissue regeneration
collagen
gelatin
glucose
glucose oxidase
hydroxyl radical
iron
nanoparticle
silver
antiinfective agent
Antibacterials
Broad spectrum
Drug-resistance
Hydroxyl radicals
Infected wounds
Wound dressings
Wound healing
animal experiment
animal model
animal tissue
Article
bacterial skin disease
biocompatibility
contact angle
controlled study
electrospinning
Escherichia coli
Escherichia coli infection
fibroblast
hair follicle
heart
high resolution scanning electron microscopy
histopathology
in vitro study
in vivo study
inflammatory cell
kidney
liver
lung
male
mouse
nonhuman
spleen
tissue regeneration
wound healing
wound infection
animal
bacterium
chemistry
microbiology
M3 - Article
PY - 2023
ST - Autocatalytically hydroxyl-producing composite wound dressing for bacteria-
infected wound healing
TI - Autocatalytically hydroxyl-producing composite wound dressing for bacteria-
infected wound healing
85156123534&doi=10.1016%2fj.nano.2023.102683&partnerID=40&md5=71acd0ba84dfe622922d9
abfea237421
VL - 51
ID - 4999
ER -
TY - JOUR
AB - More information characterizing the biological responses to nanoparticles is
needed to allow the U. S. Food and Drug Administration to evaluate the safety and
effectiveness of products with nano-scale components. The potential cytotoxicity
and inflammatory responses of Au NPs (60 nm, NIST standard reference materials)
were investigated in murine macrophages. Cytotoxicity was evaluated by MTT and LDH
assays. Cytokines (IL-6, TNF-alpha), nitric oxide, and ROS were assayed to assess
inflammatory responses. Morphological appearance and localization of particles were
examined by high resolution illumination microscopy, transmission electron
microscopy (TEM), and scanning TEM coupled with EDX spectroscopy. Results showed no
cytotoxicity and no elevated production of proinflammatory mediators; however,
imaging analyses demonstrated cellular uptake of Au NPs and localization within
intracellular vacuoles. These results suggest that 60 nm Au NPs, under the exposure
conditions tested, are not cytotoxic, nor elicit pro-inflammatory responses. The
localization of Au NPs in intracellular vacuoles suggests endosomal containment and
an uptake mechanism involving endocytosis.
AN - WOS:000294225600001
AU - Zhang, Q.
AU - Schrand, A. M.
AU - Hussain, S. M.
AU - Goering, P. L.
DA - SEP
DO - 10.3109/17435390.2010.512401
IS - 3
PY - 2011
SN - 1743-5390
1743-5404
SP - 284-295
ST - Uptake of gold nanoparticles in murine macrophage cells without cytotoxicity
or production of pro-inflammatory mediators
T2 - NANOTOXICOLOGY
TI - Uptake of gold nanoparticles in murine macrophage cells without cytotoxicity
or production of pro-inflammatory mediators
VL - 5
ID - 6127
ER -
TY - JOUR
AB - Titanium is widely used as surgical bone implants due to its excellent
mechanical properties, corrosion resistance, and good biocompatibility. However,
due to chronic inflammation and bacterial infections caused by titanium implants,
they are still at risk of failure in interfacial integration of bone implants,
severely limiting their broad clinical application. In this work, chitosan gels
crosslinked with glutaraldehyde were prepared and successfully loaded with silver
nanoparticles (nAg) and catalase nanocapsules (n (CAT)) to achieve functionalized
coating on the surface of titanium alloy steel plates. Under chronic inflammatory
conditions, n (CAT) significantly reduced the expression of macrophage tumor
necrosis factor (TNF-a), increased the expression of osteoblast alkaline
phosphatase (ALP) and osteopontin (OPN), and enhanced osteogenesis. At the same
time, nAg inhibited the growth of S. aureus and E. coli. This work provides a
general approach to functional coating of titanium alloy implants and other
scaffolding materials.
AN - WOS:000941274800001
AU - Zhang, T.
AU - Qin, X. Y.
AU - Gao, Y.
AU - Kong, D.
AU - Jiang, Y. H.
AU - Cui, X.
AU - Guo, M. T.
AU - Chen, J. Y.
AU - Chang, F. F.
AU - Zhang, M.
AU - Li, J.
AU - Yin, P. B.
C7 - 1118487
DA - FEB 15
DO - 10.3389/fbioe.2023.1118487
PY - 2023
SN - 2296-4185
ST - Functional chitosan gel coating enhances antimicrobial properties and
osteogenesis of titanium alloy under persistent chronic inflammation
TI - Functional chitosan gel coating enhances antimicrobial properties and
VL - 11
ID - 6293
ER -
TY - JOUR
AB - Titanium is widely used as surgical bone implants due to its excellent
mechanical properties, corrosion resistance, and good biocompatibility. However,
due to chronic inflammation and bacterial infections caused by titanium implants,
they are still at risk of failure in interfacial integration of bone implants,
severely limiting their broad clinical application. In this work, chitosan gels
crosslinked with glutaraldehyde were prepared and successfully loaded with silver
nanoparticles (nAg) and catalase nanocapsules (n (CAT)) to achieve functionalized
coating on the surface of titanium alloy steel plates. Under chronic inflammatory
conditions, n (CAT) significantly reduced the expression of macrophage tumor
necrosis factor (TNF-α), increased the expression of osteoblast alkaline
phosphatase (ALP) and osteopontin (OPN), and enhanced osteogenesis. At the same
time, nAg inhibited the growth of S. aureus and E. coli. This work provides a
general approach to functional coating of titanium alloy implants and other
scaffolding materials. Copyright © 2023 Zhang, Qin, Gao, Kong, Jiang, Cui, Guo,
Chen, Chang, Zhang, Li and Yin.
AU - Zhang, T.
AU - Qin, X.
AU - Gao, Y.
AU - Kong, D.
AU - Jiang, Y.
AU - Cui, X.
AU - Guo, M.
AU - Chen, J.
AU - Chang, F.
AU - Zhang, M.
AU - Li, J.
AU - Yin, P.
C7 - 1118487
DB - Scopus
DO - 10.3389/fbioe.2023.1118487
bacteriostasis
bone formation
functional coating
persistent chronic inflammatory environment
titanium alloy
Biocompatibility
Cell death
Chitosan
Corrosion resistance
Corrosion resistant coatings
Escherichia coli
Macrophages
Pathology
Phosphatases
Steel corrosion
Anti-inflammatories
Bacteriostasi
Bone formation
Chitosan gel
Chronic inflammation
Functional coating
Osteogenesis
Persistent chronic inflammatory environment
Titanium (alloys)
Titanium alloys
M3 - Article
PY - 2023
ST - Functional chitosan gel coating enhances antimicrobial properties and
T2 - Frontiers in Bioengineering and Biotechnology
TI - Functional chitosan gel coating enhances antimicrobial properties and
85149487898&doi=10.3389%2ffbioe.2023.1118487&partnerID=40&md5=7d317698d6c0d26013a2e
5fc114fe72a
VL - 11
ID - 5041
ER -
TY - JOUR
AB - It remains challenging to cure chronic diabetic wounds due to its' harsh
microenvironment and poor tissue regeneration ability. At present, bacteria
elimination, inflammatory response suppression and angiogenesis orderly render an
important paradigm for chronic diabetic wound treatment. Herein, smart-responsive
multifunctional hydrogels were developed to improve chronic diabetic wound healing,
which could quickly respond to the acidic environment of the diabetic wound site
and mediate multistage sequential delivery of silver and curcumin-loaded
polydopamine nanoparticles (PDA@Ag&Cur NPs) and vascular endothelial growth factor
(VEGF). PDA@Ag&Cur NPs and VEGF endowed the hydrogels with antibacterial, anti-
inflammatory and angiogenesis performances, respectively. The in vitro and in vivo
experiments confirmed that our multistage drug delivery hydrogels could effectively
eliminate bacteria, relieve inflammatory response, and induce angiogenesis, hence
accelerating the closure of chronic diabetic wounds. In conclusion, we highlighted
the importance of multistage manipulation in wound healing and offered a
combinatorial therapeutic strategy to sequentially deliver drugs exactly aiming at
the dynamic wound healing stages. © 2023
AU - Zhang, W.
AU - Liu, W.
AU - Long, L.
AU - He, S.
AU - Wang, Z.
AU - Liu, Y.
AU - Yang, L.
AU - Chen, N.
AU - Hu, C.
AU - Wang, Y.
DB - Scopus
DO - 10.1016/j.jconrel.2023.01.049
KW - Combinatorial treatment
Diabetic wound healing
Multistage sequential drug delivery
Smart-responsive hydrogels
Bacteria
Diabetes Mellitus
Humans
Hydrogels
Wound Healing
Tissue regeneration
beta actin
curcumin
hydrogel
interleukin 6
silver nanoparticle
vasculotropin
antiinfective agent
vasculotropin A
Angiogenesis
Diabetic wounds
Multi-stages
Smart-responsive hydrogel
Wound healing
angiogenesis
animal experiment
animal model
aqueous solution
Article
cell migration
cell proliferation
cell viability
chronic wound
controlled study
cytotoxicity
diabetic wound
drug release
epidermal thickness
erythrocyte
human
human cell
HUVEC cell line
immunofluorescence
in vitro study
in vivo study
macrophage
nonhuman
oscillation
pH
protein expression
rat
sol-gel
survival rate
synthesis
tissue regeneration
wound care
wound closure
wound healing
wound tissue
bacterium
diabetes mellitus
pharmacology
M3 - Article
PY - 2023
SP - 821-834
ST - Responsive multifunctional hydrogels emulating the chronic wounds healing
cascade for skin repair
TI - Responsive multifunctional hydrogels emulating the chronic wounds healing
cascade for skin repair
85147090225&doi=10.1016%2fj.jconrel.2023.01.049&partnerID=40&md5=51c45829f67820c65e
e686bb24c83956
VL - 354
ID - 5056
ER -
TY - JOUR
AB - A visible-light-driven heterostructured AgI/g-C3N4 was prepared by a
deposition-precipitation method. The composition, structure, morphology, and
optical properties of the photocatalyst were characterized by Brunauer-Emmett-
Teller method (BET), X-ray powder diffraction (XRD), Fourier transform-infrared
spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), transmission electron
microscope (TEM), scanning electron microscope (SEM), UV–vis diffused reflectance
spectroscopy (DRS), photoluminescence spectroscopy (PL), photocurrent, and
electrochemical impedance spectroscopy (EIS), respectively. AgI/g-C3N4 composite
photocatalysts exhibited higher photocatalytic activities than those of AgI
nanoparticles and g-C3N4 in the degradation of diclofenac (a model anti-
inflammatory medicine) under visible light irradiation (λ ≥ 400 nm). When the mass
molar ratio of AgI was 45% in AgI/g-C3N4, the reaction rate constant of diclofenac
degradation reached 0.561 min−1, which was almost 12.5 and 43.2 times higher than
that achieved by AgI (0.045 min−1) and g-C3N4 (0.013 min−1). The h+ and O2 - were
pinpointed as the main reactive species in the photocatalytic reaction using their
obligate radical scavengers. Diclofenac was completely degraded and partly
mineralized during the photodegradation. The main intermediates were determined by
liquid chromatograph mass spectrometer (LC-MS), and toxicological assessments were
carried out to evaluate the change of toxicity in the degradation process. In
addition, the photocatalysts showed excellent stability over multiple reaction
cycles. Finally, a possible photocatalytic and charge separation mechanism was
proposed. © 2017 Elsevier Inc.
AU - Zhang, W.
AU - Zhou, L.
AU - Shi, J.
AU - Deng, H.
DB - Scopus
DO - 10.1016/j.jcis.2017.02.022
KW - AgI
Diclofenac
G-C3N4
Heterojunction
Visible light photocatalysis
Complexation
Electrochemical impedance spectroscopy
Heterojunctions
Light
Optical properties
Photocatalysis
Photocatalysts
Photodegradation
Photoluminescence spectroscopy
Precipitation (chemical)
Rate constants
Semiconductor quantum wells
Silver halides
Spectrometers
diclofenac
graphite
hydroxyl radical
nanocarrier
nanocomposite
nanosheet
organohalogen derivative
oxygen radical
scavenger
silver halide
unclassified drug
Brunauer-Emmett-Teller method
Charge separation mechanism
Deposition precipitation methods
G-C3N4
Visible light photocatalytic activity
Visible-light irradiation
Visible-light photocatalysis
Article
brunauer emmett teller method
chemical structure
constants and coefficients
controlled study
deposition precipitation method
drug degradation
drug stability
electrochemical impedance spectroscopy
human
human cell
investigative procedures
light
MTT assay
multiple reaction monitoring
nanofabrication
nanopharmaceutics
optics
photocatalysis
photodegradation
photoluminescence
priority journal
reaction rate constant
scanning electron microscope
surface area
M3 - Article
PY - 2017
SP - 167-176
ST - Fabrication of novel visible-light-driven AgI/g-C3N4 composites with enhanced
visible-light photocatalytic activity for diclofenac degradation
T2 - Journal of Colloid and Interface Science
TI - Fabrication of novel visible-light-driven AgI/g-C3N4 composites with enhanced
visible-light photocatalytic activity for diclofenac degradation
85013224748&doi=10.1016%2fj.jcis.2017.02.022&partnerID=40&md5=cccd4764cd9557783e5a8
b76a279a47f
VL - 496
ID - 5476
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have attracted increased interest and are
currently used in various industries including medicine, cosmetics, textiles,
electronics, and pharmaceuticals, owing to their unique physical and chemical
properties, particularly as antimicrobial and anticancer agents. Recently, several
studies have reported both beneficial and toxic effects of AgNPs on various
prokaryotic and eukaryotic systems. To develop nanoparticles for mediated therapy,
several laboratories have used a variety of cell lines under in vitro conditions to
evaluate the properties, mode of action, differential responses, and mechanisms of
action of AgNPs. In vitro models are simple, cost-effective, rapid, and can be used
to easily assess efficacy and performance. The cytotoxicity, genotoxicity, and
biocompatibility of AgNPs depend on many factors such as size, shape, surface
charge, surface coating, solubility, concentration, surface functionalization,
distribution of particles, mode of entry, mode of action, growth media, exposure
time, and cell type. Cellular responses to AgNPs are different in each cell type
and depend on the physical and chemical nature of AgNPs. This review evaluates
significant contributions to the literature on biological applications of AgNPs. It
begins with an introduction to AgNPs, with particular attention to their overall
impact on cellular effects. The main objective of this review is to elucidate the
reasons for different cell types exhibiting differential responses to nanoparticles
even when they possess similar size, shape, and other parameters. Firstly, we
discuss the cellular effects of AgNPs on a variety of cell lines; Secondly, we
discuss the mechanisms of action of AgNPs in various cellular systems, and try to
elucidate how AgNPs interact with different mammalian cell lines and produce
significant effects; Finally, we discuss the cellular activation of various
signaling molecules in response to AgNPs, and conclude with future perspectives on
research into AgNPs.
AN - WOS:000387768300183
AU - Zhang, X. F.
AU - Shen, W.
AU - Gurunathan, S.
C7 - 1603
DA - OCT
DO - 10.3390/ijms17101603
IS - 10
PY - 2016
SN - 1422-0067
ST - Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in
Vitro Model
TI - Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in
Vitro Model
VL - 17
ID - 6103
ER -
TY - JOUR
AB - With the wide application of silver nanoparticles (AgNPs), their potential
damage to human health needs to be investigated. Lung is one of the main target
organs after inhalation of AgNPs. Naringenin has been reported to have anti-
inflammatory and anti-oxidative properties. This study aims to evaluate the
protective effects of naringenin against AgNPs-induced lung injury and determine
the underlying mechanism. In in vivo experiments, AgNPs were intratracheally
instilled into ICR mice (l mg/kg) to establish a lung injury model. These mice were
then treated with naringenin by oral gavage (25, 50, 100 mg/kg) for three days.
Naringenin treatment decreased the levels of white blood cells, neutrophils, and
lymphocytes in the blood, ameliorated lung injury, suppressed the release of pro-
inflammatory cytokines, normalized ferroptotic markers and prevented oxidative
stress with elevating Nrf2 and HO-1 protein expressions in lung. In in vitro
experiments, BEAS-2B cells were firstly treated with AgNPs (320 mu g/mL) and then
naringenin (25, 50, and 100 mu M), respectively. Naringenin attenuated AgNPsinduced
oxidative stress and inflammatory response. Moreover, naringenin attenuated AgNPs-
induced apoptosis with modulated low BAX, CytC, cleaved Caspase9, cleaved Caspase3
but high Bcl2. Furthermore, naringenin effectively decreased ferroptotic markers
and increased the protein expressions of Nrf2 and HO-1, as well as increased the
nuclear translocation of Nrf2. Importantly, the anti-apoptotic and anti-ferroptotic
effects of naringenin in BEAS-2B cells were found to be at least partially Nrf2-
dependent. These results indicated that naringenin exerted anti-inflammation, anti-
apoptosis, and anti-ferroptosis effects and protected against AgNPsinduced lung
injury at least partly via activating Nrf2/HO-1 signaling pathway.
AN - WOS:000891202900002
AU - Zhang, X. X.
AU - Li, M.
AU - Wu, H.
AU - Fan, W. Y.
AU - Zhang, J. S.
AU - Su, W. W.
AU - Wang, Y. G.
AU - Li, P. B.
C6 - NOV 2022
C7 - 121127
DA - DEC 15
DO - 10.1016/j.lfs.2022.121127
PY - 2022
SN - 0024-3205
1879-0631
ST - Naringenin attenuates inflammation, apoptosis, and ferroptosis in silver
nanoparticle-induced lung injury through a mechanism associated with Nrf2/HO-1
axis: In vitro and in vivo studies
T2 - LIFE SCIENCES
TI - Naringenin attenuates inflammation, apoptosis, and ferroptosis in silver
VL - 311
ID - 6081
ER -
TY - JOUR
AB - Curcumin is a polyphenolic substance extracted from plants such as Curcuma
longa, Curcuma zedoaria, and radix curcumae, and it has attracted much attention
because of the anti-inflammatory, antioxidant, anti-tumor, anti-bacterial and other
multiple pharmacological effects. Cervical cancer is one of the most common
malignant tumors in women. With the application of HPV (human papillomavirus)
vaccine, the incidence of cervical cancer is expected to be reduced, but it remains
difficult to promote the vaccine among low-income population. As a commonly used
food additive, curcumin has recently been found to have a significant therapeutic
effect in the treatment of cervical cancer. In recent years, numerous in vitro and
in vivo studies have found that curcumin can have significant efficacy in anti-
cervical cancer treatment by promoting apoptosis, inhibiting tumour cell pro-
liferation, metastasis and invasion, inhibiting HPV and inducing autophagy in
tumour cells. However, due to poor water solubility, rapid catabolism, and low
bioavailability of curcumin, studies on curcumin derivatives and novel formulations
are increasing. Curcumin has a wide range of mechanisms of action against cervical
cancer and may become a novel antitumor drug in the future, opening up new ideas
for the research of curcumin in the field of antitumor. There is a lack of
systematic reviews on the mechanism of action of curcumin against cervical cancer.
Therefore, this study is a review of the literature based on the mechanism of
action of curcumin against cervical cancer, with a view to providing reference
information for scientific and clinical practitioners.
AN - WOS:000961542400001
AU - Zhang, X. Y.
AU - Zhu, L.
AU - Wang, X. Z.
AU - Zhang, H. R.
AU - Wang, L. Z.
AU - Xia, L.
C6 - MAR 2023
C7 - 114590
DA - JUN
DO - 10.1016/j.biopha.2023.114590
PY - 2023
SN - 0753-3322
1950-6007
ST - Basic research on curcumin in cervical cancer: Progress and perspectives
TI - Basic research on curcumin in cervical cancer: Progress and perspectives
VL - 162
ID - 6682
ER -
TY - JOUR
AB - Oral amalgam tattoos (AT) are distinct pigmentations of the oral mucosa
resulting from accidental incorporation of dental amalgam in the oral soft tissues.
Dental amalgams and in particular mercury, one of the constituents of dental
amalgams, have for long been considered toxic. Oral ATs are easily accessable to
study soft tissue reaction to amalgam and its degradation products. In this study,
17 oral ATs were examined by transmission electron microscopy and energy dispersive
X-ray microanalysis. Ultrastructurally, in the ATs, three kinds of electron-dense
particles were observed. The largest particles ranged in size from 0.5 up to
several 100 μm. Smaller electron-dense inclusions (0.5-0.1 μm) were seen
extracellularly associated with meshworks of elastic fibers and collagen bundles.
The third and smallest type of particles (5-30 nm in diameter) was found with
basement membranes of small vessels and pericytes and particularly decorating
collagen bundles. Element analysis regularly revealed the presence of silver,
sulphur, copper and lead in the AT decay products. Mercury was found in only one
instance. Tissue reactions due to ATs seem to be minimal. No acute inflammatory
changes were seen. Larger inclusions occasionally were surrounded by macrophages
and multinucleated cells. TEM and element analysis may in specific cases be helpful
in the differential diagnosis of pigmented lesions of the oral mucosa. © 2006
AU - Zhang, X.
AU - Gelderblom, H. R.
AU - Zierold, K.
AU - Reichart, P. A.
DB - Scopus
DO - 10.1016/j.micron.2006.07.020
IS - 5
KW - Amalgam tattoos
Degradation
Electron microscopy
Electron-dense decoration/precipitation
Histopathology
X-ray element analysis
Dental Amalgam
Electron Probe Microanalysis
Foreign Bodies
Humans
Metals, Heavy
Microscopy, Electron
Mouth Mucosa
Particle Size
Tattooing
Dental amalgams
Mercury (metal)
Precipitation (chemical)
Tissue
Toxicity
X ray analysis
dental alloy
heavy metal
article
chemistry
electron microscopy
electron probe microanalysis
foreign body
human
metabolism
mouth mucosa
particle size
pathology
tattooing
Pigments
M3 - Article
PY - 2007
SP - 543-548
ST - Morphological findings and energy dispersive X-ray microanalysis of oral
amalgam tattoos
T2 - Micron
TI - Morphological findings and energy dispersive X-ray microanalysis of oral
amalgam tattoos
33947236729&doi=10.1016%2fj.micron.2006.07.020&partnerID=40&md5=fbe94f63df13c95c1e8
f1f7dbe2dbf22
VL - 38
ID - 5763
ER -
TY - JOUR
AB - With the wide application of silver nanoparticles (AgNPs), their potential
damage to human health needs to be investigated. Lung is one of the main target
organs after inhalation of AgNPs. Naringenin has been reported to have anti-
inflammatory and anti-oxidative properties. This study aims to evaluate the
protective effects of naringenin against AgNPs-induced lung injury and determine
the underlying mechanism. In in vivo experiments, AgNPs were intratracheally
instilled into ICR mice (l mg/kg) to establish a lung injury model. These mice were
then treated with naringenin by oral gavage (25, 50, 100 mg/kg) for three days.
Naringenin treatment decreased the levels of white blood cells, neutrophils, and
lymphocytes in the blood, ameliorated lung injury, suppressed the release of pro-
inflammatory cytokines, normalized ferroptotic markers and prevented oxidative
stress with elevating Nrf2 and HO-1 protein expressions in lung. In in vitro
experiments, BEAS-2B cells were firstly treated with AgNPs (320 μg/mL) and then
naringenin (25, 50, and 100 μM), respectively. Naringenin attenuated AgNPs-induced
oxidative stress and inflammatory response. Moreover, naringenin attenuated AgNPs-
induced apoptosis with modulated low BAX, CytC, cleaved Caspase9, cleaved Caspase3
but high Bcl2. Furthermore, naringenin effectively decreased ferroptotic markers
and increased the protein expressions of Nrf2 and HO-1, as well as increased the
nuclear translocation of Nrf2. Importantly, the anti-apoptotic and anti-ferroptotic
effects of naringenin in BEAS-2B cells were found to be at least partially Nrf2-
dependent. These results indicated that naringenin exerted anti-inflammation, anti-
apoptosis, and anti-ferroptosis effects and protected against AgNPs-induced lung
injury at least partly via activating Nrf2/HO-1 signaling pathway. © 2022 Elsevier
Inc.
AU - Zhang, X.
AU - Li, M.
AU - Wu, H.
AU - Fan, W.
AU - Zhang, J.
AU - Su, W.
AU - Wang, Y.
AU - Li, P.
C7 - 121127
DB - Scopus
DO - 10.1016/j.lfs.2022.121127
KW - Lung injury
Naringenin
Nrf2/HO-1 pathway
Animals
GA-Binding Protein Transcription Factor
Heme Oxygenase-1
Humans
Inflammation
Lung Injury
Metal Nanoparticles
Mice
Mice, Inbred ICR
Oxidative Stress
Silver
caspase 3
caspase 9
cytochrome c
ferric ion
glutathione
hoe 33342
interleukin 10
interleukin 1beta
interleukin 33
interleukin 6
naringenin
nicotinamide adenine dinucleotide phosphate
phospholipid hydroperoxide glutathione peroxidase
prostaglandin E2
protein Bax
protein bcl 2
silver nanoparticle
GA binding protein
heme oxygenase 1
metal nanoparticle
silver
animal experiment
animal model
animal tissue
apoptosis
apoptosis assay
Article
bicinchoninic acid assay
controlled study
cytokine release
densitometry
experimental lung injury
ferroptosis
flow cytometry
human
human cell
in vitro study
in vivo study
inflammation
leukocyte count
lipid peroxidation assay
lymphocyte count
male
mouse
MTS assay
neutrophil count
nonhuman
Nrf2 signaling
optical density
oxidative stress
protein expression
radioimmunoprecipitation
RNA extraction
silver nanoparticle induced lung injury
Western blotting
animal
lung injury
metabolism
M3 - Article
PY - 2022
ST - Naringenin attenuates inflammation, apoptosis, and ferroptosis in silver
T2 - Life Sciences
TI - Naringenin attenuates inflammation, apoptosis, and ferroptosis in silver
85141664191&doi=10.1016%2fj.lfs.2022.121127&partnerID=40&md5=66874d43159dd0c9d7b97e
0fc1384391
VL - 311
ID - 5013
ER -
TY - JOUR
AB - Neural tissue engineering is a research field aimed at rebuilding
neurological defects resulting from severe trauma, vascular impairment,
syringomyelia, spinal stenosis, malignant and benign tumors, or transverse
myelitis. Of particular interest, neural stem cells (NSCs) and the effective
differentiation and proliferation thereof are attractive research areas that have
yielded widespread utility for implants or neural scaffold materials. Graphene and
its derivatives have more effective and efficient physical, chemical, and
biological abilities than other nanomaterials, and may act as new coating materials
to promote neuronal proliferation and differentiation. Therefore, here, we review
the recent progress of studies that examine the effect of graphenebased materials
on NSCs. We specifically review how graphene and its derivatives influence NSC
adhesion, differentiation, and proliferation. We also discuss the risks of
graphene-based materials, including their anti-inflammatory effects, in the realm
of neural tissue engineering as well as current challenges facing the field today.
AN - WOS:000591527900001
AU - Zhang, Y.
AU - Wang, S.
AU - Yang, P.
C7 - 2519105
DA - JUL 3
DO - 10.1155/2020/2519105
PY - 2020
SN - 1687-4110
1687-4129
ST - Effects of Graphene-Based Materials on the Behavior of Neural Stem Cells
T2 - JOURNAL OF NANOMATERIALS
TI - Effects of Graphene-Based Materials on the Behavior of Neural Stem Cells
VL - 2020
ID - 6444
ER -
TY - JOUR
AB - The rhizome of A. officinarum possesses immense pharmaceutical properties
like antioxidant, anti-inflammatory, antiapoptotic, anticancer activities. The
foremost downside of herbal-based drugs is their poor bioavailability, to trounce
this we synthesized a herbal based silver nanodrug with Alpinia officinarum rhizome
extract and assessed its effect against the cisplatin-induced nephrotoxicity in in
vivo model. The A. officinarum biosynthesized silver nanoparticles (AG-AO) were
characterized using UV-Spec, FTIR, XRD, TEM and SAED analysis. The antioxidant and
the nephroprotective property of biosynthesized AG-AO nanoparticles were assessed
by estimating the levels of kidney biomarkers, cytokine, inflammatory markers, free
radicals and antioxidants induced in control and experimental. Antiapoptotic effect
of AG-AO nanoparticles were evaluated by measuring the levels of apoptotic proteins
in control and experimental rats. Further, it is confirmed with histopathological
analysis of kidney tissue with haematoxylin and eosin staining. Our physical
analysis confirms the biosynthesized silver nanoparticles by A. officinarum and it
satisfies the qualities of potent nanoparticles to be used for medication. Our
biochemical, molecular and histopathological results confirm the antioxidant,
antiapoptotic, anti-inflammatory properties of AG-AO. Overall our results
authentically confirm AG-AO is a potent nephroprotective drug, which can be a
supplementary drug to prevent cisplatin-induced nephrotoxicity. © 2019, © 2019 The
Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
AU - Zhang, Z.
AU - Xin, G.
AU - Zhou, G.
AU - Li, Q.
AU - Veeraraghavan, V. P.
AU - Krishna Mohan, S.
AU - Wang, D.
AU - Liu, F.
DB - Scopus
DO - 10.1080/21691401.2019.1645158
IS - 1
KW - Alpinia officinarum
Cisplatin
green synthesis
nephroprotectant
nephrotoxicity
Alpinia
Animals
Apoptosis
Biomarkers
Cytokines
Cytoprotection
Down-Regulation
Kidney
Male
Metal Nanoparticles
Oxidative Stress
Rats
Rats, Wistar
Silver
Antioxidants
Biochemistry
Free radicals
Metal nanoparticles
Quality control
albumin
cisplatin
creatinine
cytokine
free radical
protein bcl 2
protein p53
silver nanoparticle
urea
biological marker
metal nanoparticle
silver
Cis-platin
Green synthesis
Nephrotoxicity
albumin blood level
animal experiment
animal model
animal tissue
apoptosis
Article
controlled study
diffraction
drug efficacy
drug synthesis
glomerulus filtration rate
histopathology
immunoblotting
in vivo study
kidney tissue
lipid peroxidation
male
nonhuman
osmotic pressure
oxidative stress
protein urine level
proteinuria
rat
renal protection
urea blood level
X ray diffraction
animal
cell protection
chemistry
cytology
down regulation
drug effect
green chemistry
kidney
metabolism
Wistar rat
M3 - Article
PY - 2019
SP - 3212-3221
ST - Green synthesis of silver nanoparticles from Alpinia officinarum mitigates
cisplatin-induced nephrotoxicity via down-regulating apoptotic pathway in rats
TI - Green synthesis of silver nanoparticles from Alpinia officinarum mitigates
cisplatin-induced nephrotoxicity via down-regulating apoptotic pathway in rats
85069954081&doi=10.1080%2f21691401.2019.1645158&partnerID=40&md5=7583729d2f7c0b1a79
f9ff09383ed5f3
VL - 47
ID - 5362
ER -
TY - JOUR
AB - Increasing evidence has revealed that neuroinflammation plays a pivotal role
in axonal injures. Nucleotide oligomerization domain (NOD)-like receptor protein
(NLRP3) inflammasome is reported to be widely involved with the pathology of
central nervous system disorders. But the role of NLRP3 in diffuse axonal injury
(DAI) are rarely reported. The purpose of this study was to investigate the
expression of NLRP3 after diffuse axonal injury and the role of NLRP3 in axonal
injures. The lateral head rotation device was used to establish DAI model of rats.
Immunohistochemical staining for beta-amyloid precursor protein and Bielschowsky
silver staining were used to assess axonal injures and axonal loss. Terminal
Deoxynucleotidyl Transferase-Mediated Digoxigenin-dUTP-Biotin Nick-End Labelling
Assay was used to detect cell apoptosis. Brain water content was used to assess
cerebral edema and the modified Neurologic Severity Score was used to assess the
neurological deficits. Components of NLRP3 inflammasome, such as NLRP3, apoptosis-
associated speck-like (ASC) adapter protein and caspase-1, and pro-inflammatory
cytokines, for example IL-18 and IL-1 beta, were over-expressed in early stages of
DAI. MCC950, a selective small-molecule inhibitor of NLRP3 inflammasome, inhibited
the over-expression of NLRP3 inflammasome and pro-inflammatory cytokines after DAI.
MCC950 alleviated axonal injures and cell apoptosis. MCC950 also decreased brain
water content and alleviated neurologic deficits 1 day and 3 days after DAI but not
7 days after DAI. These results suggest that MCC950 treatment in the early stages
of DAI has a time limiting effect in preventing from axonal injuries and
neurological deficits, and that NLRP3 inflammasome plays an important role in
axonal injures and may be a potential candidate for axonal injures following DAI.
AN - WOS:000536447300001
AU - Zhao, J. J.
AU - Guo, X. Y.
AU - Wang, B.
AU - Yang, Z. B.
AU - Huang, T. Q.
AU - Guo, D.
AU - Zhang, M.
AU - Song, J. N.
C6 - MAY 2020
DA - SEP
DO - 10.1007/s11064-020-03063-6
IS - 9
PY - 2020
SN - 0364-3190
1573-6903
SP - 2020-2031
ST - MCC950 Inhibits NLRP3 Inflammasome and Alleviates Axonal Injures in Early
Stages of Diffuse Axonal Injury in Rats
T2 - NEUROCHEMICAL RESEARCH
TI - MCC950 Inhibits NLRP3 Inflammasome and Alleviates Axonal Injures in Early
VL - 45
ID - 6723
ER -
TY - JOUR
AB - Increasing evidence has revealed that neuroinflammation plays a pivotal role
in axonal injures. Nucleotide oligomerization domain (NOD)-like receptor protein
(NLRP3) inflammasome is reported to be widely involved with the pathology of
central nervous system disorders. But the role of NLRP3 in diffuse axonal injury
(DAI) are rarely reported. The purpose of this study was to investigate the
expression of NLRP3 after diffuse axonal injury and the role of NLRP3 in axonal
injures. The lateral head rotation device was used to establish DAI model of rats.
Immunohistochemical staining for β-amyloid precursor protein and Bielschowsky
silver staining were used to assess axonal injures and axonal loss. Terminal
Deoxynucleotidyl Transferase-Mediated Digoxigenin-dUTP-Biotin Nick-End Labelling
Assay was used to detect cell apoptosis. Brain water content was used to assess
cerebral edema and the modified Neurologic Severity Score was used to assess the
neurological deficits. Components of NLRP3 inflammasome, such as NLRP3, apoptosis-
associated speck-like (ASC) adapter protein and caspase-1, and pro-inflammatory
cytokines, for example IL-18 and IL-1β, were over-expressed in early stages of DAI.
MCC950, a selective small-molecule inhibitor of NLRP3 inflammasome, inhibited the
over-expression of NLRP3 inflammasome and pro-inflammatory cytokines after DAI.
MCC950 alleviated axonal injures and cell apoptosis. MCC950 also decreased brain
water content and alleviated neurologic deficits 1 day and 3 days after DAI but not
7 days after DAI. These results suggest that MCC950 treatment in the early stages
of DAI has a time limiting effect in preventing from axonal injuries and
neurological deficits, and that NLRP3 inflammasome plays an important role in
axonal injures and may be a potential candidate for axonal injures following DAI. ©
2020, Springer Science+Business Media, LLC, part of Springer Nature.
AU - Zhao, J.
AU - Guo, X.
AU - Wang, B.
AU - Yang, Z.
AU - Huang, T.
AU - Guo, D.
AU - Zhang, M.
AU - Song, J.
DB - Scopus
DO - 10.1007/s11064-020-03063-6
IS - 9
KW - Diffuse axonal injury
Inflammasome
MCC950
NLRP3
Animals
Apoptosis
Axons
Brain Edema
Cerebral Cortex
Heterocyclic Compounds, 4 or More Rings
Inflammasomes
Inflammation
Male
Rats
Sulfones
adaptor protein
apoptosis associated speck like adapter protein
cryopyrin
inflammasome
interleukin 18
interleukin 1beta
mcc 950
protein inhibitor
unclassified drug
fused heterocyclic rings
MCC-950
Nlrp3 protein, rat
sulfone
adult
animal cell
animal experiment
animal model
apoptosis
Article
axonal injury
brain edema
brain water
controlled study
cytokine response
disease severity assessment
male
modified neurologic severity score
mouse
nonhuman
priority journal
protein function
animal
axon
brain cortex
complication
drug effect
inflammation
metabolism
pathology
rat
M3 - Article
PY - 2020
SP - 2020-2031
ST - MCC950 Inhibits NLRP3 Inflammasome and Alleviates Axonal Injures in Early
T2 - Neurochemical Research
TI - MCC950 Inhibits NLRP3 Inflammasome and Alleviates Axonal Injures in Early
85085547636&doi=10.1007%2fs11064-020-03063-
6&partnerID=40&md5=63ba3ea062f93df9fc6ba446d6912358
VL - 45
ID - 5313
ER -
TY - JOUR
AB - Polymorphonuclear neutrophils (PMN) are one fraction of the major
inflammatory cells in allergic asthma (asthma, in short); the role of PMN in the
asthma pathogenesis is not fully understood yet. This study aims to investigate the
effects of specific Ag-guiding exosomes on suppressing the neutrophil-dominant
airway inflammation. In this study, BALB/c mice were immunized with ovalbumin plus
complete Freund adjuvant to induce an asthma model featured with neutrophil-
dominant lung inflammation. The Ag specific PMN (sPMN)-targeting exosomes (tExo),
that were exosomes carrying a complex of specific Ag/anti-CD64 Ab and Fas ligand,
were constructed to be used to alleviate neutrophilic asthma in mice. We found that
sPMNs were the major cellular component in bronchoalveolar lavage fluid (BALF) in
asthma mice, while less than 3% PMNs in naive control mice. The sPMNs expressed
higher levels of CD64, which formed complexes with Ag-specific IgG (sIgG). The
sIgG/CD64 complex-carrying PMNs could be activated upon exposing to specific Ags.
Exposure to tExos induced Ag-specific PMNs apoptosis. Administration of tExos
efficiently suppressed experimental asthma. We conclude that a fraction of sPMN was
identified in the airway of asthma mice. The sPMNs could be activated upon exposure
to specific Ags. tExos could induce sPMNs apoptosis, that show the translational
potential in the treatment of asthma. © 2020 Elsevier Ltd
AU - Zhao, M. Z.
AU - Li, Y.
AU - Han, H. Y.
AU - Mo, L. H.
AU - Yang, G.
AU - Liu, Z. Q.
AU - Ma, C.
AU - Yang, P. C.
AU - Liu, S.
DB - Scopus
DO - 10.1016/j.molimm.2020.11.005
KW - Allergy
Asthma
Exosome
Immunotherapy
Neutrophil
Animals
Antibodies
Antigens
Apoptosis
Drug Carriers
Exosomes
Freund's Adjuvant
Hypersensitivity
Immunoglobulin G
Lung
Mice
Mice, Inbred BALB C
Nanoparticles
Neutrophils
Ovalbumin
Pneumonia
Receptors, IgG
Vaccines
CD63 antigen
CD64 antigen
CD81 antigen
CD9 antigen
Fas ligand
Freund adjuvant
gamma interferon
interleukin 13
interleukin 4
interleukin 5
ovalbumin
silver
antibody
antigen
drug carrier
Fc receptor
immunoglobulin G
nanoparticle
vaccine
allergic asthma
animal cell
animal experiment
animal model
animal tissue
apoptosis
Article
bone marrow derived dendritic cell
controlled study
exosome
immunoprecipitation
immunotherapy
lung parenchyma
mouse
neutrophil
nonhuman
pneumonia
priority journal
animal
asthma
Bagg albino mouse
hypersensitivity
immunology
lung
M3 - Article
PY - 2021
SP - 103-111
ST - Specific Ag-guiding nano-vaccines attenuate neutrophil-dominant allergic
asthma
T2 - Molecular Immunology
TI - Specific Ag-guiding nano-vaccines attenuate neutrophil-dominant allergic
asthma
85096590572&doi=10.1016%2fj.molimm.2020.11.005&partnerID=40&md5=0bd35ceee865b4b5578
299c6147b0732
VL - 129
ID - 5346
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) based antibacterial materials are widely applied
to commodity and clinic wound treatments. However, genotoxicity and inflammatory
response induced by AgNPs inhibit their application as the antibacterial coating of
medical devices like catheters. A novel gelatin-AgNPs coating manufacture method
was introduced here to generate an antibacterial coating, which nearly immunes to
inflammatory, on basal PHBV material. The novel gelatin-AgNPs coating was produced
by immobilizing gelatin on the Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)
membrane and subsequently fixing AgNPs on acquired gelatin coating. Prepared
gelatin-AgNPs coatings displayed considerable antibacterial capacity. These
gelatin-AgNPs coatings did not cause inflammation, growth inhibition or apoptosis
to normal human embryonic lung fibroblasts, MRC-5 cells, by analyzing the
transcription levels of relevant genes in these cells incubated with tested
coatings for 4 days. Hence, this novel gelatin-AgNPs coating manufacture method
paved its way to apply in medical devices manufacture including catheters. (C) 2016
AN - WOS:000385598100007
AU - Zhao, X. X.
AU - Liu, H. R.
AU - Hu, Y. B.
AU - Huang, J. Y.
AU - Zhang, S. H.
AU - Ja, F.
DA - OCT
DO - 10.1016/j.reactfunctpolym.2016.07.014
PY - 2016
SN - 1381-5148
1873-166X
SP - 54-59
ST - A novel gelatin-AgNPs coating preparing method for fabrication of
antibacterial and no inflammation inducible coatings on PHBV
T2 - REACTIVE & FUNCTIONAL POLYMERS
TI - A novel gelatin-AgNPs coating preparing method for fabrication of
VL - 107
ID - 6308
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) based antibacterial materials are widely applied
to commodity and clinic wound treatments. However, genotoxicity and inflammatory
response induced by AgNPs inhibit their application as the antibacterial coating of
medical devices like catheters. A novel gelatin-AgNPs coating manufacture method
was introduced here to generate an antibacterial coating, which nearly immunes to
inflammatory, on basal PHBV material. The novel gelatin-AgNPs coating was produced
by immobilizing gelatin on the Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)
membrane and subsequently fixing AgNPs on acquired gelatin coating. Prepared
gelatin-AgNPs coatings displayed considerable antibacterial capacity. These
gelatin-AgNPs coatings did not cause inflammation, growth inhibition or apoptosis
to normal human embryonic lung fibroblasts, MRC-5 cells, by analyzing the
transcription levels of relevant genes in these cells incubated with tested
coatings for 4 days. Hence, this novel gelatin-AgNPs coating manufacture method
paved its way to apply in medical devices manufacture including catheters. © 2016
Elsevier B.V.
AU - Zhao, X.
AU - Liu, H.
AU - Hu, Y.
AU - Huang, J.
AU - Zhang, S.
AU - Ja, F.
DB - Scopus
DO - 10.1016/j.reactfunctpolym.2016.07.014
KW - AgNPs
Antibacterial
Biocompatibility
Coating
Gelatin
Catheters
Cell culture
Cell death
Manufacture
Pathology
Silver
Transcription
Antibacterial coatings
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Coatings
M3 - Article
PY - 2016
SP - 54-59
ST - A novel gelatin-AgNPs coating preparing method for fabrication of
T2 - Reactive and Functional Polymers
TI - A novel gelatin-AgNPs coating preparing method for fabrication of
84985937179&doi=10.1016%2fj.reactfunctpolym.2016.07.014&partnerID=40&md5=eddfa91ae7
cd5d662ecbbcbba42f2f73
VL - 107
ID - 5501
ER -
TY - JOUR
AB - Objective. We have previously demonstrated that inflammation induced by toll-
like receptors (TLRs) 2/4 exert cerebral deleterious effects after diffuse axonal
injury (DAI); however, the underlying mechanisms are not fully understood.
Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine involved
in inflammatory responses. The purpose of this study was to investigate the role of
MIF in inflammation induced by TLRs in the cortices of DAI rats.Methods. The rat
DAI model was established by head rotational acceleration and confirmed by beta-
APP, HE, and silver staining. MIF protein expression at 3 h, 6 h, 12 h, 1 d, and 3
d after DAI was measured by western blot. The localization of MIF was measured by
immunofluorescence. MIF antagonist ISO-1 was intracerebroventricularly injected to
inhibit MIF. Neuronal and axonal injury and glial responses were assessed by TUNEL,
immunohistochemistry, and TEM. Expression of TLR2, TLR4, ERK, phospho-ERK, NF-kappa
B, and phospho-NF-kappa B was examined by western blot. The level of IL-1 beta, IL-
6, and TNF-alpha was measured by ELISA.Results. MIF expression was significantly
increased, peaking at 1 day after DAI, and MIF was mainly localized in microglial
cells and neurons. ISO-1 suppressed neuronal apoptosis, axonal injury, and glial
responses and decreased the expression of downstream signaling molecules related to
TLR2/4, including ERK, phospho-ERK, NF-kappa B, phospho-NF-kappa B, IL-1 beta, IL-
6, and TNF-alpha.Conclusion. MIF was involved in the neuronal and axonal damage
through a TLR-related pathway following DAI.
AN - WOS:000574699200006
AU - Zhao, Y. L.
AU - Wei, X.
AU - Li, W. M.
AU - Shan, C. Y.
AU - Song, J. N.
AU - Zhang, M.
C7 - 5946205
DA - SEP 7
DO - 10.1155/2020/5946205
PY - 2020
SN - 2314-6133
2314-6141
ST - Inhibition of Macrophage Migration Inhibitory Factor Protects against
Inflammation through a Toll-like Receptor-Related Pathway after Diffuse Axonal
Injury in Rats
T2 - BIOMED RESEARCH INTERNATIONAL
TI - Inhibition of Macrophage Migration Inhibitory Factor Protects against
Injury in Rats
VL - 2020
ID - 6816
ER -
TY - JOUR
AB - Objective. We have previously demonstrated that inflammation induced by toll-
like receptors (TLRs) 2/4 exert cerebral deleterious effects after diffuse axonal
injury (DAI); however, the underlying mechanisms are not fully understood.
Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine involved
in inflammatory responses. The purpose of this study was to investigate the role of
MIF in inflammation induced by TLRs in the cortices of DAI rats. Methods. The rat
DAI model was established by head rotational acceleration and confirmed by β-APP,
HE, and silver staining. MIF protein expression at 3 h, 6 h, 12 h, 1 d, and 3 d
after DAI was measured by western blot. The localization of MIF was measured by
immunofluorescence. MIF antagonist ISO-1 was intracerebroventricularly injected to
inhibit MIF. Neuronal and axonal injury and glial responses were assessed by TUNEL,
immunohistochemistry, and TEM. Expression of TLR2, TLR4, ERK, phospho-ERK, NF-κB,
and phospho-NF-κB was examined by western blot. The level of IL-1β, IL-6, and TNF-α
was measured by ELISA. Results. MIF expression was significantly increased, peaking
at 1 day after DAI, and MIF was mainly localized in microglial cells and neurons.
ISO-1 suppressed neuronal apoptosis, axonal injury, and glial responses and
decreased the expression of downstream signaling molecules related to TLR2/4,
including ERK, phospho-ERK, NF-κB, phospho-NF-κB, IL-1β, IL-6, and TNF-α.
Conclusion. MIF was involved in the neuronal and axonal damage through a TLR-
related pathway following DAI. © 2020 Yonglin Zhao et al.
AU - Zhao, Y.
AU - Wei, X.
AU - Li, W.
AU - Shan, C.
AU - Song, J.
AU - Zhang, M.
C7 - 5946205
DB - Scopus
DO - 10.1155/2020/5946205
KW - Animals
Apoptosis
Inflammation
Interleukin-1beta
Interleukin-6
Intramolecular Oxidoreductases
Macrophage Migration-Inhibitory Factors
Microglia
Neuroglia
NF-kappa B
Rats
Signal Transduction
Toll-Like Receptors
3 (4 hydroxyphenyl) 4,5 dihydro 5 isoxazole acetic acid methyl ester
cytokine receptor antagonist
interleukin 1beta
interleukin 6
macrophage migration inhibition factor
toll like receptor
unclassified drug
isomerase
Mif protein, rat
animal experiment
animal model
animal tissue
Article
astrocyte
brain cortex
controlled study
histopathology
immunofluorescence
microglia
nerve cell
neuroapoptosis
neuroprotection
nonhuman
protein expression
protein function
rat
silver staining
TLR signaling
TUNEL assay
upregulation
Western blotting
animal
apoptosis
glia
inflammation
metabolism
physiology
signal transduction
Sprague Dawley rat
M3 - Article
PY - 2020
ST - Inhibition of Macrophage Migration Inhibitory Factor Protects against
Injury in Rats
TI - Inhibition of Macrophage Migration Inhibitory Factor Protects against
Injury in Rats
85091562321&doi=10.1155%2f2020%2f5946205&partnerID=40&md5=77c9623cc6d35cfd59cb3ef31
4925102
VL - 2020
ID - 5357
ER -
TY - JOUR
AB - Psoriasis, an autoimmune inflammatory skin disorder, is one of the commonest
immune-mediated disease conditions affecting individuals globally. At the moment,
the conventional methods applied against psoriasis treatment have various drawbacks
involving limited efficacy, skin irritation, immunosuppression, etc. Therefore, it
is important for scientists to find a more potent and alternative drug approach
towards psoriasis therapeutics. Natural medicine still remains an important source
for new drug discovery due to its therapeutical significance in various drug
administration routes. However, the traditional formulation of topical therapies
for psoriasis is limited in efficacy, which limits the use of natural medicine.
Based on the aforementioned limitations, the use of nanocarriers in preparation of
these topical herbal products could be tremendously beneficial in enhancing the
efficacy of topical medications. Growing pieces of evidence have proposed that the
utilization of nanocarriers in transdermal preparation as a prospective technique,
with regards to better potency, directs drug absorption to site of action, and
minimum toxicity effect respectively. In the course of this review, we emphasized
the pathological mechanism of psoriasis, natural medicine formula, active
components of natural medicine, and nanopreparations used in the treatment of
psoriasis. Graphical abstract: [Figure not available: see fulltext.]. © 2021,
Controlled Release Society.
AU - Zhao, Z.
AU - Liu, T.
AU - Zhu, S.
AU - Pi, J.
AU - Guo, P.
AU - Qi, D.
AU - Liu, Z.
AU - Li, N.
DB - Scopus
DO - 10.1007/s13346-021-01031-3
IS - 6
KW - Nanoparticles
Natural medicine
Psoriasis
Topical drug delivery system
Administration, Cutaneous
Drug Carriers
Humans
Prospective Studies
3 (3,4 dihydroxyphenyl)lactic acid
aurantiin
autoantigen
baicalein
baicalin
capsaicin
celastrol
convallatoxin
Cornus officinalis extract
curcumin
cycloastragenol
cytokine
diosgenin
geraniol
ginsenoside
Glycyrrhiza glabra root
gold nanoparticle
herbaceous agent
interleukin 17
interleukin 19
interleukin 1beta
interleukin 23
interleukin 6
khellactone
liposome
messenger RNA
moutan cortex
nanocarrier
nanoparticle
natural product
niosome
oxymatrine
paclitaxel
psoralen
resveratrol
silver nanoparticle
turmeric
unclassified drug
withanolide
drug carrier
Acorus
Angelica sinensis
Artemisia capillaris
bilayer membrane
capillary endothelial cell
cell cycle arrest
Clematis
Cornus officinalis
cream
cytokine release
drug absorption
drug efficacy
Forsythia suspensa
Fritillaria cirrhosa
fruit
HaCat cell line
herb
herbal medicine
human
immune system
keratinocyte
Lonicera
Mentha
nanomedicine
nonhuman
ointment
pathogenesis
Pogostemon cablin
prescription
psoriasis
Review
Scrophularia
Scutellaria baicalensis
skin irritation
topical treatment
cutaneous drug administration
prospective study
M3 - Review
PY - 2022
SP - 1326-1338
ST - Natural medicine combined with nanobased topical delivery systems: a new
strategy to treat psoriasis
T2 - Drug Delivery and Translational Research
TI - Natural medicine combined with nanobased topical delivery systems: a new
strategy to treat psoriasis
85110768736&doi=10.1007%2fs13346-021-01031-
3&partnerID=40&md5=1c080c2b7b781e8ed86a84b9e1f6f87c
VL - 12
ID - 5136
ER -
TY - JOUR
AB - Novel neural interfaces capable of reliably capturing electrical signals are
crucial for the development of prostheses. Longitudinal intrafascicular electrodes
(LIFEs) have been proposed as a promising technology, and their feasibility and
biocompatibility need to be investigated for long-term implantation. In this study,
custom-designed 95%Pt–5%Ir intrafascicular electrodes were implanted into the
sciatic nerves of 14 rabbits using our novel direct microsurgical technique. The
biocompatibility and their ability to record electrophysiological signals were
serially investigated up to 9 months after implantation. Nerve tissues were
examined using light and transmitted electron microscopy, and axon diameters were
quantified, evaluated over time, and compared with sham-control (N = 4). Selective
stimulation and stable recording properties of electrical signals were achieved by
intrafascicular electrodes along the experimental period. While
electrophysiological signal amplitude decreased by as early as 1 month after
implantation (p < 0.05), the signal strength recovered to baseline levels by 3–5
months (p > 0.05). Axon diameter results showed a similar trend of initial decline
(10.8% reduction, p < 0.01) followed by gradual recovery by 6 months (p > 0.05).
Microstructural and ultrastructural analysis revealed modest tissue damage at the
implantation site after implantation with gradual normalization over time.
Intrafascicular electrodes implanted with direct microsurgical techniques
demonstrated good biocompatibility and have great potential for long-term
implantation and electrophysiological recordings. Though subtle tissue damage
impaired ability to capture electrophysiological signals in the first 2 months,
this damage gradually normalized after 3 months, and was fully normalized by 6
months. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater,
107B: 435–444, 2019. © 2018 Wiley Periodicals, Inc.
AU - Zhen, G.
AU - Chen, H.
AU - Tsai, S. Y.
AU - Zhang, J.
AU - Chen, T.
AU - Jia, X.
DB - Scopus
DO - 10.1002/jbm.b.34135
IS - 2
electrophysiology
intrafascicular electrodes
peripheral nerve
Animals
Feasibility Studies
Materials Testing
Rabbits
Sciatic Nerve
Biocompatibility
Electrophysiology
Tissue
ammonia
eosin
hematoxylin
Electrical signal
Electrophysiological recordings
Implantation sites
Neural interfaces
Peripheral nerves
Recording properties
Selective stimulations
Ultrastructural analysis
animal cell
animal experiment
Article
axon
collagen fibril
connective tissue
controlled study
electrode implantation
electron microscopy
electrophysiological procedures
giant cell
image analysis
inflammation
inflammatory cell
information processing
latent period
Leporidae
lymphocyte
microsurgery
myelin sheath
myelinated nerve
nervous tissue
neutrophil
nonhuman
phagocytosis
plasma cell
scar tissue
Schwann cell
sciatic nerve
silver staining
animal
electrode implant
feasibility study
materials testing
metabolism
surgery
Electrodes
M3 - Article
PY - 2019
SP - 435-444
ST - Long-term feasibility and biocompatibility of directly microsurgically
implanted intrafascicular electrodes in free roaming rabbits
TI - Long-term feasibility and biocompatibility of directly microsurgically
implanted intrafascicular electrodes in free roaming rabbits
85045840647&doi=10.1002%2fjbm.b.34135&partnerID=40&md5=c9d049dd34af69186f87aa6f55b4
3189
VL - 107
ID - 5405
ER -
TY - JOUR
AB - In clinical practice, we noticed that triple negative breast cancer (TNBC)
patients had higher shear-wave elasticity (SWE) stiffness than non-TNBC patients
and a higher α-SMA expression was found in TNBC tissues than the non-TNBC tissues.
Moreover, SWE stiffness also shows a clear correlation to neoadjuvant response
efficiency. To elaborate this phenomenon, TNBC cell membrane-modified polylactide
acid-glycolic acid (PLGA) nanoparticle was fabricated to specifically deliver
artesunate to regulate SWE stiffness through inhibiting CAFs functional status. As
tested in MDA-MB-231 and E0771 orthotopic tumor models, CAFs functional status
inhibited by 231M-ARS@PLGA nanoparticles (231M-AP NPs) had reduced the SWE
stiffness as well as attenuated hypoxia of tumor as tumor soil loosening agent
which amplified the antitumor effects of paclitaxel and PD1 inhibitor. Single-cell
sequencing indicated that the two main CAFs (extracellular matrix and wound healing
CAFs) that produces extracellular matrix could influence the tumor SWE stiffness as
well as the antitumor effect of drugs. Further, biomimetic nanoparticles inhibited
CAFs function could attenuate tumor hypoxia by increasing proportion of
inflammatory blood vessels and oxygen transport capacity. Therefore, our finding is
fundamental for understanding the role of CAFs on affecting SWE stiffness and drugs
antitumor effects, which can be further implied in the potential clinical
theranostic predicting in neoadjuvant therapy efficacy through non-invasive
analyzing of SWE imaging. © 2022 The Authors
AU - Zheng, D.
AU - Zhou, J.
AU - Qian, L.
AU - Liu, X.
AU - Chang, C.
AU - Tang, S.
AU - Zhang, H.
AU - Zhou, S.
DB - Scopus
DO - 10.1016/j.bioactmat.2022.10.025
KW - Biomimetic nanoparticles
Cancer-associated fibroblasts
Shear-wave elasticity imaging
Theranostic prediction
artesunate
CD3 antigen
CD4 antigen
CD47 antigen
CD8 antigen
discoidin domain receptor 1
galectin 1
gelatinase A
gelatinase B
Ki 67 antigen
matrix metalloproteinase 14
nanoparticle
paclitaxel
silver nanoparticle
Smad protein
vimentin
angiogenesis
animal model
animal tissue
apoptosis
Article
bioinformatics
blood vessel
cancer associated fibroblast
cancer patient
cancer recurrence
CD8+ T lymphocyte
cell infiltration
cell membrane
cell proliferation
clinical practice
controlled study
cytotoxicity
dispersity
down regulation
drug loading capacity
drug mechanism
echography
endothelium cell
EO771 cell line
female
flow cytometry
follow up
Fourier transform mass spectrometry
functional status
gene expression
gene identification
half life time
human
human cell
human tissue
image analysis
imaging stiffness
immune response
immunofluorescence
lymph node metastasis
metastasis
microarray analysis
microscopy
mouse
multimodal imaging
natural killer cell
neoadjuvant therapy
nonhuman
oxygen transport
particle size
phagocytosis
protein expression
quality control
shear wave elastography
single cell analysis
T-47 cell line
tissue microarray
tumor associated leukocyte
tumor invasion
tumor volume
tumor weight
tumor xenograft
TUNEL assay
Western blotting
wound healing
M3 - Article
PY - 2023
SP - 567-587
ST - Biomimetic nanoparticles drive the mechanism understanding of shear-wave
elasticity stiffness in triple negative breast cancers to predict clinical
treatment
TI - Biomimetic nanoparticles drive the mechanism understanding of shear-wave
elasticity stiffness in triple negative breast cancers to predict clinical
treatment
85141307167&doi=10.1016%2fj.bioactmat.2022.10.025&partnerID=40&md5=f0365a5f4bfc2c85
8d6d5448f5f099cd
VL - 22
ID - 4987
ER -
TY - JOUR
AB - Increasing utilization of stabilized iron sulfides (FeS) nanoparticles
implies an elevated release of the materials into the environment. To understand
potential impacts and underlying mechanisms of nanoparticle-induced stress, we used
the transcriptome sequencing (RNA-seq) technique to characterize the
transcriptomesfrom adult zebrafish exposed to 10 mg/L carboxymethyl cellulose (CMC)
stabilized FeS nanoparticles for 96 h, demonstrating striking differences in the
gene expression profiles in liver. The exposure caused significant expression
alterations in genes related to immune and inflammatory responses, detoxification,
oxidative stress and DNA damage/repair. The complement and coagulation cascades
Kyoto encyclopedia of genes and genomes (KEGG) pathway was found significantly up-
regulated under nanoparticle exposure. The quantitative real-time polymerase chain
reaction using twelve genes confirmed the RNA-seq results. We identified several
candidate genes commonly regulated in liver, which may serve as gene indicators
when exposed to the nanoparticles. Hepatic inflammation was further confirmed by
histological observation of pyknotic nuclei, and vacuole formation upon exposure.
Tissue accumulation tests showed a 2.2 times higher iron concentration in the fish
tissue upon exposure. This study provides preliminary mechanistic insights into
potential toxic effects of organic matter stabilized FeS nanoparticles, which will
improve our understanding of the genotoxicity caused by stabilized nanoparticles.
AN - WOS:000432928200008
AU - Zheng, M.
AU - Lu, J. G.
AU - Zhao, D. Y.
C7 - 8083
DA - MAY 24
DO - 10.1038/s41598-018-26499-x
PY - 2018
SN - 2045-2322
ST - Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish
in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide
Nanoparticles
TI - Toxicity and Transcriptome Sequencing (RNA-seq) Analyses of Adult Zebrafish
in Response to Exposure Carboxymethyl Cellulose Stabilized Iron Sulfide
Nanoparticles
VL - 8
ID - 6656
ER -
TY - JOUR
AB - Rhei Radix et Rhizoma, also known as rhubarb or Da Huang, has been widely
used as a spice and as traditional herbal medicine for centuries, and is currently
marketed in China as the principal herbs in various prescriptions, such as Da-
Huang-Zhe-Chong pills and Da-Huang-Qing-Wei pills. Emodin, a major bioactive
anthraquinone derivative extracted from rhubarb, represents multiple health
benefits in the treatment of a host of diseases, such as immune-inflammatory
abnormality, tumor progression, bacterial or viral infections, and metabolic
syndrome. Emerging evidence has made great strides in clarifying the multi-
targeting therapeutic mechanisms underlying the efficacious therapeutic potential
of emodin, including anti-inflammatory, immunomodulatory, anti-fibrosis, anti-
tumor, anti-viral, anti-bacterial, and anti-diabetic properties. This comprehensive
review aims to provide an updated summary of recent developments on these
pharmacological efficacies and molecular mechanisms of emodin, with a focus on the
underlying molecular targets and signaling networks. We also reviewed recent
attempts to improve the pharmacokinetic properties and biological activities of
emodin by structural modification and novel material-based targeted delivery. In
conclusion, emodin still has great potential to become promising therapeutic
options to immune and inflammation abnormality, organ fibrosis, common malignancy,
pathogenic bacteria or virus infections, and endocrine disease or disorder.
Scientifically addressing concerns regarding the poor bioavailability and vague
molecular targets would significantly contribute to the widespread acceptance of
rhubarb not only as a dietary supplement in food flavorings and colorings but also
as a health-promoting TCM in the coming years. © 2021, The Author(s).
AU - Zheng, Q.
AU - Li, S.
AU - Li, X.
AU - Liu, R.
C7 - 102
DB - Scopus
DO - 10.1186/s13020-021-00509-z
IS - 1
KW - Emodin
Modification
Pharmacokinetics
Pharmacology
Toxicology
11beta hydroxysteroid dehydrogenase 1
adenylate kinase
anthraquinone derivative
caspase 3
cremophor
cryopyrin
daunorubicin
decitabine
emodin
flavoring agent
fluorouracil
gamma interferon
glucose
interleukin 4
interleukin 6
liposome
macrogol 400
nicotinamide
paclitaxel
poloxamer
polyethyleneimine
polymer
polyvinylsulfonate
protein Bax
purinergic P2X7 receptor
rhubarb extract
scleroprotein
silver nanoparticle
sorafenib
unclassified drug
acute lung injury
acute pancreatitis
antifibrotic activity
antiviral activity
bacterial infection
cataract
Chinese medicine
Coxsackievirus B3
cyclic voltammetry
density functional theory
diabetes mellitus
diabetic neuropathy
dietary supplement
drug efficacy
drug elimination
drug mechanism
drug potentiation
drug solubility
drug targeting
Enterovirus A71
fatty liver
food color
health promotion
human
immunomodulation
immunopathology
inflammation
Influenza A virus
liver cell carcinoma
liver toxicity
malignant neoplasm
metastasis inhibition
molecular mechanics
nephrotoxicity
nonhuman
protein expression
Review
rhubarb
rotating disk electrode voltammetry
signal transduction
Streptococcus suis
virus infection
M3 - Review
PY - 2021
ST - Advances in the study of emodin: an update on pharmacological properties and
mechanistic basis
T2 - Chinese Medicine (United Kingdom)
TI - Advances in the study of emodin: an update on pharmacological properties and
mechanistic basis
85116724721&doi=10.1186%2fs13020-021-00509-
z&partnerID=40&md5=1b9ba87a7316efa6a42e53ea3002dd6f
VL - 16
ID - 5175
ER -
TY - JOUR
AB - BACKGROUND: Nanosilver has significant antibacterial properties, and
nanohydroxyapatite has good biological activity and mechanical strength, while
their mixture cannot only promote bone formation but also have antibacterial
properties. OBJECTIVE: To observe the effect of nanosilver and nanohydroxyapatite
mixed filling on the osseointegration of immediate implants. METHODS: Nine New
Zealand white rabbits were randomly divided into experimental group (n=6) and
control group (n=3) after four incisors from the upper and lower jaw were
extracted. The mixture of nanosilver and nanohydroxyapatite was filled into the
tooth socket in the experimental group, while nanohydroxyapatite was filled into
the tooth socket in the control group. Titanium screw was immediately implanted
into both groups. The intact maxilla and mandibular specimens were harvested at the
4th, 8th, and 12th weeks after operation. Gross observation, X-ray bone density
analysis, torque test and histological observation were conducted. RESULTS AND
CONCLUSION: The gray value and maximum torque value of regenerated osseous tissue
at different time points in experimental group were significantly higher than those
in the control group (P < 0.05). Within 12 weeks of implantation, the bone
formation rate and maturity of new bone tissue were higher in the experimental
group compared with the control group, and no inflammatory cell infiltration
occurred. At the 4th week after implantation, there was a large amount of
inflammatory cell infiltration, and few inflammatory existed at the 8th week after
implantation. These results demonstrate that compared with nanohydroxyapatite
alone, the mixture of nanosilver and nanohydroxyapatite shows better antibacterial
effect, biocompatibility and osteoinductive ability, which may accelerate
osseointegration and promote osteogenesis. © 2015, Journal of Clinical
Rehabilitative Tissue Engineering Research. All rights reserved.
AU - Zheng, Y.
AU - Li, B. T.
AU - Wang, L. J.
AU - Fan, S. F.
AU - Hou, X. W.
DB - Scopus
DO - 10.3969/j.issn.2095-4344.2015.47.019
IS - 47
KW - Durapatite
Nanostructures
Osseointegration
Tissue Engineering
hydroxyapatite
silver nanoparticle
titanium
animal cell
animal experiment
animal tissue
Article
biocompatibility
bone density
bone development
bone implant
bone regeneration
bone tissue
cell infiltration
controlled study
experimental rabbit
histology
incisor
inflammatory cell
mandible
maxilla
nonhuman
oral screw
ossification
tooth extraction
tooth socket
torque
M3 - Article
PY - 2015
SP - 7649-7654
ST - Effect of nanosilver and nanohydroxyapatite mixed filling on osseointegration
of immediate implants
T2 - Chinese Journal of Tissue Engineering Research
TI - Effect of nanosilver and nanohydroxyapatite mixed filling on osseointegration
of immediate implants
85018791447&doi=10.3969%2fj.issn.2095-
4344.2015.47.019&partnerID=40&md5=e44a0c1c91f9d73f4656622a6cac565d
VL - 19
ID - 5647
ER -
TY - JOUR
AB - Objective. To study bioinertness of new nanosized carbon-containing in
compounds in experiment. Methods. The pulsed vacuum arc method was used to deposite
the coating of nitinol wire. Nitinol wire stents were implanted in the parenchymal
organs (kidney and liver) and in the choledoch of sexually mature Wistar rats. The
animals were taken out of the experiment on the 14th and 30th day after
implantation. The thickness of developing reactive tissue areas was measured; the
cytoarchitectonics around the implants was evaluated. Results. In the cases of
implantation in the parenchymal organs the quantitative and qualitative
characteristics depended on the implantation site have been defined. Inflammatory
changes around implants in the liver proved to be 1,5-2 fold more intensive than in
the kidneys the same period of observation. Qualitative analysis of
cytoarchitectonics around the implants in different groups has not revealed
statistically significant differences depending on the implant material. The
quantitative assessment of inflammatory infiltration in the group with
nanostructured coating based on amorphous carbon matrix with including of silver
nanoscale clusters was less intense regardless of the implantation site.
Implantation of new carbon nanoscale materials into the rat choledoch lumen has not
followed by necrobiotic changes and perforation of its wall; no mortality was
observed in the study groups. The reaction of the extrahepatic biliary tract to the
introduction of implant such homologous when it implanted in parenchymal organs.
Conclusion. The materials being developed for implantation showed no cytotoxicity.
The best indicators of bioinertness were registered in the group of implants with
nanostructural coating based on amorphous carbon matrix with inclusions of
nanoscale clusters of silver. This result can be explained by the inertia of the
carbon to the tissues and by antiproliferative properties of silver.
AU - Zhernakova, N. I.
AU - Dolzhikov, A. A.
AU - Bocharova, K. A.
AU - Kolpakov, A. J.
AU - Dmitriem, V. N.
DB - Scopus
DO - 10.18484/2305-0047.2015.5.491
IS - 5
KW - Antiproliferative properties of silver
Biomedical materials
Cytotoxicity
Implant
Inflammation
Pulsed vacuum arc method
Stent
M3 - Article
PY - 2015
SP - 491-499
ST - Assessment of cytotoxicity of carbon-containing nanosized coatings
T2 - Novosti Khirurgii
TI - Assessment of cytotoxicity of carbon-containing nanosized coatings
84949425844&doi=10.18484%2f2305-
0047.2015.5.491&partnerID=40&md5=db95909544eda5824b48a597cbaedeab
VL - 23
ID - 5635
ER -
TY - JOUR
AB - The study investigates cytokines secretion ability of leukocytic cells
stimulated by materials contained in implants. The stimulated cytokine-production
activity in relation to the cytokines (IFNα, IFNγ, TNFα, IL-1ß, IL-1βRa, IL-2, IL-
6, IL-8) was changed in the presence of and depended on the type of the studied
implant. The most intensive inflammatory reaction was observed in case with metals
and polyurethane, the least intensive one was registered in the presence of a
nanostructural coating based on amorphous carbon and silver nanoparticles (NPs:Ag).
AU - Zhernakova, N. I.
AU - Dolzhykov, A. A.
AU - Bocharova, K. A.
AU - Dmitriyev, V. N.
AU - Kolpakov, A. Y.
AU - Manokhin, S. S.
AU - Miroshnichenko, O. V.
AU - Liubushkin, A. V.
DB - Scopus
IS - 6
KW - Cytokines
Inflammation
Medical implant
alpha interferon
gamma interferon
interleukin 1beta
interleukin 2
interleukin 6
nanocoating
silver nanoparticle
Article
biocompatibility
cell stimulation
controlled study
cytokine production
cytokine release
in vitro study
leukocyte
nanoanalysis
surface property
M3 - Article
PY - 2014
SP - 1477-1480
ST - Study of nanostructural coating biocompatibility in-vitro
TI - Study of nanostructural coating biocompatibility in-vitro
84911064921&partnerID=40&md5=81c45dad7ff361930e0d9009fd269bb6
VL - 5
ID - 5586
ER -
TY - JOUR
AB - Background: Voriconazole is the traditionally used antifungal agent, but its
ophthalmic form is unsatisfactory. A novel ophthalmic drug delivery system with
biomedical devices may be of promising for the prognosis of fungal keratitis.
Objective: This study was to investigate the sustained release, therapeutic effect
and biocompatibility of effect and quaternized chitosan functionalized with
carboxylated graphene and nano-silver and voriconazole (CS-ETA/Ag/GO/Vor) for
fungal keratitis. Methods: This study complied with the Regulations for the
Administration of Affair Concerning Experimental Animals of State Science and
Technology Commission. Two hundred and ten SPF female C57BL/6 mice were selected
with the age 6-8 weeks for the biocompatibility experiment (30 mice) and
therapeutic observation of CS-ETA/Ag/GO/Vor (180 mice). CS-ETA/Ag/GO and
CS-ETA/Ag/GO/Vor were attached on the normal corneas of mice and compared with the
normal mice to assess the histopathological changes. Aspergillus fumigatus-infected
mouse models were established in the left eyes of 180 mice by intrastromally
injection of 2.0 μL Aspergillus fumigatus suspension with the density of 5 × 107
CFU/ml, then the mice were randomized into the model control group, CS-ETA/Ag/GO
group and CS-ETA/Ag/GO/Vor group, and the corresponding membrane were attached the
central corneas in different groups. In 1 day, 3, 5, 7 days after modeling, the
corneas were examined under the slit lamp microscope and scored, and corneal
sections were prepared for the histopathological examination. Fungal activity was
confirmed by plate counts, and real-time PCR was employed to assay the relative
expressions of interleukin-1β (IL-1β) mRNA and tumor necrosis factor-α (TNF-α) mRNA
in the corneas. Results: No morphological abnormality was seen in the corneas in
the normal control group, CS-ETA/Ag/GO group and CS-ETA/Ag/GO/Vor group. Corneal
inflammatory score was significantly lower in the CS-ETA/Ag/GO/Vor group in various
time points, with a significant differences among the groups and time points
(Fgroup=237.29, P=0.00; Ftime=260.33, P=0.00). The edema, necrosis or perforation
of cornea were seen in the model control group, and slighter inflammatory response
in the CS-ETA/Ag/GO group, and corneal edema was gradually disappear in the CS-
ETA/Ag/GO/Vor group. The corneal fungal loads were highest in the model control
group and lowest in the CS-ETA/Ag/GO/ Vor group, with significant differences among
the three groups and various time points (Fgroup=113.15, P=0.00; Ftime=126.52,
P=0.00). The relative expressions of IL-1β mRNA and TNF-α mRNA in the corneas
peaked in the fifth day after modeling in all of the three groups, and the
expression levels of IL-1β mRNA and TNF-α mRNA in the corneas were lowest in the
CS-ETA/Ag/GO/Vor group, showing significant differences among the groups and time
points (IL-1β: Fgroup=189.90, P=0.00; Ftime=108.56, P=0.00; TNF-α: Fgroup=82.55,
P=0.00; Ftime=44.36, P=0.00). Conclusions: CS-ETA/Ag/GO/Vor delivery system plays
an anti-fungal activity in fungal keratitis by the synergistic effect of
voriconazole and Ag+. In addition, CS-ETA/Ag/GO/Vor appears to have a good safety
after topical application. Copyright © 2015 by the Chinese Medical Association.
AU - Zhong, J.
AU - Yuan, J.
AU - Jiang, G.
AU - Chen, G.
AU - Sun, Y.
AU - Chen, L.
AU - Deng, Y.
DB - Scopus
DO - 10.3760/cma.j.issn.2095-0160.2015.05.006
IS - 5
KW - Antifungal agents/therapeutic use
Keratitis
Mice, inbred C57BL
Nanomedicine
Silver/pharmacology
Voriconazole
chitosan
graphene
interleukin 1beta
messenger RNA
silver nanoparticle
voriconazole
animal experiment
animal model
animal tissue
Article
aspergillosis
biocompatibility
carboxylation
controlled study
cornea
cornea edema
cornea necrosis
cornea perforation
drug efficacy
drug safety
female
histopathology
keratitis
keratomycosis
mouse
nonhuman
plate count
protein expression
slit lamp
M3 - Article
PY - 2015
SP - 412-418
ST - Therapeutic efficacy and safety of nano-silver membrane with voriconazole for
Aspergillus fumigatus keratitis after topical application
T2 - Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology
TI - Therapeutic efficacy and safety of nano-silver membrane with voriconazole for
Aspergillus fumigatus keratitis after topical application
84930067376&doi=10.3760%2fcma.j.issn.2095-
0160.2015.05.006&partnerID=40&md5=69006484d18c01f580103dd335785746
VL - 33
ID - 5584
ER -
TY - JOUR
AB - The damaging effects of nanoparticles were hypothesized to be the oxidative
stress caused by the formation of reactive oxygen species and initiation of
inflammatory reactions. In this context a study on the effects of nanosilver
particles on the formation of reactive oxygen species in human lymphocyte culture
was carried out. The obtained results showed that fluorescence intensity
considerably increased after cells had interacted with nanosilver particles of
varying concentrations, indicating the formation of reactive oxygen species and
their accumulation in lymphocyte cells. Morphological study of the lymphocyte cells
under the effects of nanosilver particles showed that the change in morphology
depends on the concentration and size of nanosilver particles: for a size ≤20 nm
the lymphocyte cell significantly shrank with pronounced differences in the
morphological structure of the cell membrane, but for a size ≥200 nm no change was
observed. © 2015 Vietnam Academy of Science & Technology
AU - Zhornik, A.
AU - Baranova, L.
AU - Volotovski, I.
AU - Chizhik, S.
AU - Drozd, E.
AU - Sudas, M.
AU - Ngo, Q. B.
AU - Nguyen, H. C.
AU - Huynh, T. H.
AU - Dao, T. H.
C7 - 025003
DB - Scopus
DO - 10.1088/2043-6262/6/2/025003
IS - 2
KW - Lymphocyte
Nanosilver particle
Oxidative stress
Toxicity
Cell culture
Oxygen
Damaging effects
Fluorescence intensities
Human lymphocytes
Morphological structures
Morphological study
Nano silver
Lymphocytes
M3 - Article
PY - 2015
ST - Interaction of nanosilver particles with human lymphocyte cells
T2 - Advances in Natural Sciences: Nanoscience and Nanotechnology
TI - Interaction of nanosilver particles with human lymphocyte cells
84922310912&doi=10.1088%2f2043-
6262%2f6%2f2%2f025003&partnerID=40&md5=96f1fae9f62686d9af69645e5d308e1b
VL - 6
ID - 5610
ER -
TY - JOUR
AB - Objective: We hypothesize that introduction of nano-silver particles to
porcine-derived small intestinal submucosa (NS-PSIS) would lead to significant
enhancement in antibacterial property in repairing contaminated abdominal defect.
Background: Porcine-derived small intestinal submucosa (PSIS) is an acellular and
xenogenic biological material intensively used in repairing and regenerating
wounded and dysfunctional tissues. Surgical site infection (SSI) remains so far a
serious problem and major challenge, particularly in contaminated tissue-deficient
repairing. Methods: Self-assembly was used to fabricate NS-PSIS. The antibacterial
property was evaluated in vitro and in vivo by means of repairing full-thickness
contaminated abdominal defect in rats. The native PSIS and polypropylene-oxidized
regenerated cellulose were served as controls. In addition, changes in
biomechanical resistance, morphology and immunohistochemistry for inflammatory
reaction and neovasculation in the repaired abdominal wall were analyzed. Biosafety
was investigated by pyrogen test, skin irritation test and silver measurement in
vivo. Results: NS-PSIS exhibited strong antibacterial activity against
Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and
Pseudomonas aeruginosa on agar diffusion, with mean diameters of inhibition zone
ranging from 11.9 to 23.5 mm. There were significantly lower SSI incidence and a
tendency of better abdominal wall resistance in the NS-PSIS group as compared with
the PSIS or polypropylene-oxidized regenerated cellulose group after repairing
contaminated abdominal defect in rats. Nano-silver modified PSIS did not change the
native PSIS property in the tissue recolonization, remodeling and
neovascularization. NS-PSIS was not pyrogenic or skin irritated, without silver
residual in vivo after repairing contaminated abdominal defect. Conclusion: Nano-
silver particles to PSIS lead to significant enhancements in antibacterial property
in vitro and in vivo without decreasing its biomechanical resistance and
biocompatibility. This study provides proof of concept for the use of nano-silver
modified naturally derived PSIS as an ideal scaffold for SSI prevention in the
contaminated tissue-deficient repair.
AN - WOS:000289510600029
AU - Zhou, H. Y.
AU - Zhang, J.
AU - Yan, R. L.
AU - Wang, Q. A.
AU - Fan, L. Y.
AU - Zhang, Q.
AU - Wang, W. J.
AU - Hu, Z. Q.
DA - MAY
DO - 10.1097/SLA.0b013e31821260f3
IS - 5
PY - 2011
SN - 0003-4932
1528-1140
SP - 1033-1041
ST - Improving the Antibacterial Property of Porcine Small Intestinal Submucosa by
Nano-Silver Supplementation A Promising Biological Material to Address the Need for
Contaminated Defect Repair
T2 - ANNALS OF SURGERY
TI - Improving the Antibacterial Property of Porcine Small Intestinal Submucosa by
Nano-Silver Supplementation A Promising Biological Material to Address the Need for
Contaminated Defect Repair
VL - 253
ID - 6498
ER -
TY - JOUR
AB - Objective: We hypothesize that introduction of nano-silver particles to
porcine-derived small intestinal submucosa (NS-PSIS) would lead to significant
enhancement in antibacterial property in repairing contaminated abdominal defect.
Background: Porcine-derived small intestinal submucosa (PSIS) is an acellular and
xenogenic biological material intensively used in repairing and regenerating
wounded and dysfunctional tissues. Surgical site infection (SSI) remains so far a
serious problem and major challenge, particularly in contaminated tissue-deficient
repairing. Methods: Self-assembly was used to fabricate NS-PSIS. The antibacterial
property was evaluated in vitro and in vivo by means of repairing full-thickness
contaminated abdominal defect in rats. The native PSIS and polypropylene-oxidized
regenerated cellulose were served as controls. In addition, changes in
biomechanical resistance, morphology and immunohistochemistry for inflammatory
reaction and neovasculation in the repaired abdominal wall were analyzed. Biosafety
was investigated by pyrogen test, skin irritation test and silver measurement in
vivo. Results: NS-PSIS exhibited strong antibacterial activity against
Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and
Pseudomonas aeruginosa on agar diffusion, with mean diameters of inhibition zone
ranging from 11.9 to 23.5 mm. There were significantly lower SSI incidence and a
tendency of better abdominal wall resistance in the NS-PSIS group as compared with
the PSIS or polypropylene-oxidized regenerated cellulose group after repairing
contaminated abdominal defect in rats. Nano-silver modified PSIS did not change the
native PSIS property in the tissue recolonization, remodeling and
neovascularization. NS-PSIS was not pyrogenic or skin irritated, without silver
residual in vivo after repairing contaminated abdominal defect. Conclusion: Nano-
silver particles to PSIS lead to significant enhancements in antibacterial property
in vitro and in vivo without decreasing its biomechanical resistance and
biocompatibility. This study provides proof of concept for the use of nano-silver
modified naturally derived PSIS as an ideal scaffold for SSI prevention in the
contaminated tissue-deficient repair. © 2011 Lippincott Williams & Wilkins.
AU - Zhou, H. Y.
AU - Zhang, J.
AU - Yan, R. L.
AU - Wang, Q.
AU - Fan, L. Y.
AU - Zhang, Q.
AU - Wang, W. J.
AU - Hu, Z. Q.
DB - Scopus
DO - 10.1097/SLA.0b013e31821260f3
IS - 5
KW - Abdominal Wall
Animals
Biological Dressings
Intestinal Mucosa
Jejunum
Male
Random Allocation
Rats
Risk Assessment
Silver
Surgical Flaps
Surgical Wound Infection
Swine
Tensile Strength
Tissue Engineering
Wound Healing
cellulose
nanoparticle
polypropylene
silver
abdominal discomfort
abdominal disease
abdominal wall defect
agar diffusion
animal experiment
animal model
animal tissue
article
biosafety
controlled study
Escherichia coli
female
guinea pig
in vitro study
in vivo study
inflammation
male
nonhuman
priority journal
rabbit
rat
small intestine mucosa
submucosa
surgical infection
swine
M3 - Article
PY - 2011
SP - 1033-1041
ST - Improving the antibacterial property of porcine small intestinal submucosa by
nano-silver supplementation: A promising biological material to address the need
for contaminated defect repair
T2 - Annals of Surgery
TI - Improving the antibacterial property of porcine small intestinal submucosa by
nano-silver supplementation: A promising biological material to address the need
for contaminated defect repair
79954630411&doi=10.1097%2fSLA.0b013e31821260f3&partnerID=40&md5=097a6444e523e6a5758
cca918be73f4e
VL - 253
ID - 5697
ER -
TY - JOUR
AB - Although silicone implants are the most popular choice around the world for
breast augmentation, reconstruction, and revision, due to the poor antibacterial
properties and limited biocompatibility of silicone rubber (SR), one of the major
complications, capsule contracture, is a lingering problem. To overcome the two
main shortcomings, a dual ion implantation technique was applied to modify the
surface of SR with the basic skeleton element of organic matter, carbon (C) and the
broad-spectrum bactericide, silver (Ag). We present surface characterization,
toxicological effects, and evaluation of the mechanical, antibacterial and
biocompatible properties of C and Ag co-implanted SR (C/Ag-SRs). After ion
implantation, surface roughness and tensile strength of these new materials
increased. Biotoxicity was fully assessed byin vitroexperiments on human
fibroblasts andin vivoexperiments on rats, showing that the low-Ag groups met
safety standards. Both the anti-bacterial adhesion and bactericidal abilities of
C/Ag-SRs were superior to those of SR, which had few antibacterial activities,
especially againstStaphylococcus epidermidis. With respect to biocompatibility, the
adhesion of fibroblasts was promoted, while their proliferation was moderately
inhibited on ion-implanted surfaces. After subcutaneous implantation in rats for 7,
30, 90 and 180 d, the capsular thickness around C/Ag-SRs was significantly lower
than that around the SR. Additionally, there was no difference in the inflammatory
reaction after 7 d of retentionin vivobetween C/Ag-SRs and SR. The results
demonstrate that C/Ag-SRs are desirable shell materials for breast implants.
AN - WOS:000575549500001
AU - Zhou, X.
AU - Yan, R. S.
AU - Shi, X. H.
AU - Du, Y. C.
AU - Chen, Y.
AU - Yu, Y.
AU - Fan, D. L.
AU - Zhang, Y. M.
C7 - 065003
DA - NOV
DO - 10.1088/1748-605X/ab99d3
IS - 6
PY - 2020
SN - 1748-6041
1748-605X
ST - Co-effects of C/Ag dual ion implantation on enhancing antibacterial ability
and biocompatibility of silicone rubber
T2 - BIOMEDICAL MATERIALS
TI - Co-effects of C/Ag dual ion implantation on enhancing antibacterial ability
VL - 15
ID - 6368
ER -
TY - JOUR
AB - Although silicone implants are the most popular choice around the world for
breast augmentation, reconstruction, and revision, due to the poor antibacterial
properties and limited biocompatibility of silicone rubber (SR), one of the major
complications, capsule contracture, is a lingering problem. To overcome the two
main shortcomings, a dual ion implantation technique was applied to modify the
surface of SR with the basic skeleton element of organic matter, carbon (C) and the
broad-spectrum bactericide, silver (Ag). We present surface characterization,
toxicological effects, and evaluation of the mechanical, antibacterial and
biocompatible properties of C and Ag co-implanted SR (C/Ag−SRs). After ion
implantation, surface roughness and tensile strength of these new materials
increased. Biotoxicity was fully assessed by in vitro experiments on human
fibroblasts and in vivo experiments on rats, showing that the low-Ag groups met
safety standards. Both the anti-bacterial adhesion and bactericidal abilities of
C/Ag−SRs were superior to those of SR, which had few antibacterial activities,
especially against Staphylococcus epidermidis. With respect to biocompatibility,
the adhesion of fibroblasts was promoted, while their proliferation was moderately
inhibited on ion-implanted surfaces. After subcutaneous implantation in rats for 7,
30, 90 and 180 d, the capsular thickness around C/Ag−SRs was significantly lower
than that around the SR. Additionally, there was no difference in the inflammatory
reaction after 7 d of retention in vivo between C/Ag−SRs and SR. The results
demonstrate that C/Ag−SRs are desirable shell materials for breast implants. © 2020
IOP Publishing Ltd
AU - Zhou, X.
AU - Yan, R.
AU - Shi, X.
AU - Du, Y.
AU - Chen, Y.
AU - Yu, Y.
AU - Fan, D.
AU - Zhang, Y.
C7 - ab99d3
DB - Scopus
DO - 10.1088/1748-605X/ab99d3
IS - 6
KW - Antibacterial biomaterials
Breast augmentation
Capsular contracture
Ion implantation
Silicone rubber
Silver
Animals
Cell Adhesion
Cell Survival
Copper
Female
Fibroblasts
Focal Adhesion Protein-Tyrosine Kinases
Inflammation
Ions
Materials Testing
Prosthesis Design
Rats
Silicone Elastomers
Stress, Mechanical
Temperature
Tensile Strength
Adhesion
Biocompatibility
Cell culture
Organic carbon
Rubber
Silicon implants
Silicones
Surface roughness
Tensile strength
carbon
silastic
silver
antiinfective agent
biomaterial
copper
focal adhesion kinase
ion
Bactericidal ability
Dual ion implantation
Surface characterization
Toxicological effects
animal experiment
Article
bacterium adherence
biocompatibility
breast augmentation
cell adhesion
cell proliferation
cell viability
controlled study
cytotoxicity
drug safety
Escherichia coli
evaluation study
female
fibroblast
human
human cell
hyperplasia
in vitro study
in vivo study
nonhuman
observational study
rat
tensile strength
animal
cell survival
chemistry
inflammation
materials testing
mechanical stress
metabolism
prosthesis design
Sprague Dawley rat
temperature
M3 - Article
PY - 2020
ST - Co-effects of C/Ag dual ion implantation on enhancing antibacterial ability
TI - Co-effects of C/Ag dual ion implantation on enhancing antibacterial ability
85092481531&doi=10.1088%2f1748-605X
%2fab99d3&partnerID=40&md5=ffb8efa41db5c6704019dd6801fa66ce
VL - 15
ID - 5339
ER -
TY - JOUR
AB - Background: Nanoparticles (NPs) entering the biological environment could
interact with biomolecules, but little is known about the interaction between
unsaturated fatty acids (UFA) and NPs. Methods: This study used alpha-linolenic
acid (LNA) complexed to bovine serum albumin (BSA) for UFA and HepG2 cells for
hepatocytes. The interactions between BSA or LNA and ZnO NPs were studied. Results:
The presence of BSA or LNA affected the hydrodynamic size, zeta potential, UV-Vis,
fluorescence, and synchronous fluorescence spectra of ZnO NPs, which indicated an
interaction between BSA or LNA and NPs. Exposure to ZnO NPs with the presence of
BSA significantly induced the damage to mitochondria and lysosomes in HepG2 cells,
associated with an increase of intracellular Zn ions, but not intracellular
superoxide. Paradoxically, the release of inflammatory cytokine interleukin-6 (IL-
6) was decreased, which indicated the anti-inflammatory effects of ZnO NPs when BSA
was present. The presence of LNA did not significantly affect all of these
endpoints in HepG2 cells exposed to ZnO NPs and BSA. Conclusions: the results from
the present study indicated that BSA-complexed LNA might modestly interact with ZnO
NPs, but did not significantly affect ZnO NPs and BSA-induced biological effects in
HepG2 cells.
AN - WOS:000404048100020
AU - Zhou, Y. W.
AU - Fang, X.
AU - Gong, Y.
AU - Xiao, A. P.
AU - Xie, Y. X.
AU - Liu, L. L.
AU - Cao, Y.
C7 - 91
DA - APR
DO - 10.3390/nano7040091
IS - 4
PY - 2017
SN - 2079-4991
ST - The Interactions between ZnO Nanoparticles (NPs) and alpha-Linolenic Acid
(LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells
T2 - NANOMATERIALS
TI - The Interactions between ZnO Nanoparticles (NPs) and alpha-Linolenic Acid
(LNA) Complexed to BSA Did Not Influence the Toxicity of ZnO NPs on HepG2 Cells
VL - 7
ID - 6378
ER -
TY - JOUR
AB - Neuroinflammation, an inflammatory response within the central nervous system
(CNS), is a main hallmark of common neurodegenerative diseases, including
Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral
sclerosis (ALS), among others. The over-activated microglia release pro-
inflammatory cytokines, which induces neuronal death and accelerates
neurodegeneration. Therefore, inhibition of microglia over-activation and
microglia-mediated neuroinflammation has been a promising strategy for the
treatment of neurodegenerative diseases. Many drugs have shown promising
therapeutic effects on microglia and inflammation. However, the blood–brain barrier
(BBB)—a natural barrier preventing brain tissue from contact with harmful plasma
components—seriously hinders drug delivery to the microglial cells in CNS. As an
emerging useful therapeutic tool in CNS-related diseases, nanoparticles (NPs) have
been widely applied in biomedical fields for use in diagnosis, biosensing and drug
delivery. Recently, many NPs have been reported to be useful vehicles for anti-
inflammatory drugs across the BBB to inhibit the over-activation of microglia and
neuroinflammation. Therefore, NPs with good biodegradability and biocompatibility
have the potential to be developed as an effective and minimally invasive carrier
to help other drugs cross the BBB or as a therapeutic agent for the treatment of
neuroinflammation-mediated neurodegenerative diseases. In this review, we
summarized various nanoparticles applied in CNS, and their mechanisms and effects
in the modulation of inflammation responses in neurodegenerative diseases,
providing insights and suggestions for the use of NPs in the treatment of
neuroinflammation-related neurodegenerative diseases. © Copyright © 2021 Zhu, Hu,
Yu, Zhou, Wu, Tang, Qin, Fan and Wu.
AU - Zhu, F. D.
AU - Hu, Y. J.
AU - Yu, L.
AU - Zhou, X. G.
AU - Wu, J. M.
AU - Tang, Y.
AU - Qin, D. L.
AU - Fan, Q. Z.
AU - Wu, A. G.
C7 - 683935
DB - Scopus
DO - 10.3389/fphar.2021.683935
KW - blood-brain barrier
central neural system
nanoparticles
neurodegenerative diseases
neuroinflammation
aluminum oxide nanoparticle
arginase 1
cobalt nanoparticle
dendrimer
gold nanoparticle
graphene quantum dot
interleukin 10
interleukin 12
interleukin 1beta
interleukin 23
interleukin 6
liposome
manganese oxide nanoparticle
nanogel
nanoparticle
silica nanoparticle
silver nanoparticle
unclassified drug
Alzheimer disease
astrocytosis
attention disturbance
diffuse Lewy body disease
frontotemporal dementia
human
Huntington chorea
memory disorder
nanoemulsion
neuroprotection
neurotoxicity
nonhuman
Parkinson disease
Review
M3 - Review
PY - 2021
ST - Nanoparticles: A Hope for the Treatment of Inflammation in CNS
TI - Nanoparticles: A Hope for the Treatment of Inflammation in CNS
85107521954&doi=10.3389%2ffphar.2021.683935&partnerID=40&md5=9a316a9311f82b634fba5f
08ce89afc8
VL - 12
ID - 5226
ER -
TY - JOUR
AB - Nanomedicine is seen as a potential central player in the delivery of
personalized medicine. Biocompatibility issues of nanoparticles have largely been
resolved over the past decade. Despite their tremendous progress, less than 1% of
applied nanosystems can hit their intended target location, such as a solid tumor,
and this remains an obstacle to their full ability and potential with a high
translational value. Therefore, achieving immune-tolerable, blood-compatible, and
biofriendly nanoparticles remains an unmet need. The translational success of
nanoformulations from bench to bedside involves a thorough assessment of their
design, compatibility beyond cytotoxicity such as immune toxicity, blood
compatibility, and immune-mediated destruction/rejection/clearance profile. Here,
we report a one-pot process-engineered synthesis of ultrasmall gold nanoparticles
(uGNPs) suitable for better body and renal clearance delivery of their payloads. We
have obtained uGNP sizes of as low as 3 nm and have engineered the synthesis to
allow them to be accurately sized (almost nanometer by nanometer). The synthesized
uGNPs are biocompatible and can easily be functionalized to carry drugs, peptides,
antibodies, and other therapeutic molecules. We have performed in vitro cell
viability assays, immunotoxicity assays, inflammatory cytokine analysis, a
complement activation study, and blood coagulation studies with the uGNPs to
confirm their safety. These can help to set up a long-term safety-benefit framework
of experimentation to reveal whether any designed nanoparticles are immune-
tolerable and can be used as payload carriers for next-generation vaccines,
chemotherapeutic drugs, and theranostic agents with better body clearance ability
and deep tissue penetration.
AN - WOS:000657202500009
AU - Zhu, G. H.
AU - Azharuddin, M.
AU - Islam, R.
AU - Rahmoune, H.
AU - Deb, S.
AU - Kanji, U.
AU - Das, J.
AU - Osterrieth, J.
AU - Aulakh, P.
AU - Ibrahim-Hashi, H.
AU - Manchanda, R.
AU - Nilsson, P. H.
AU - Mollnes, T. E.
AU - Bhattacharyya, M.
AU - Islam, M. M.
AU - Hinkula, J.
AU - Slater, N. K. H.
AU - Patra, H. K.
C6 - MAY 2021
DA - MAY 26
DO - 10.1021/acsami.1c02834
IS - 20
PY - 2021
SN - 1944-8244
1944-8252
SP - 23410-23422
ST - Innate Immune Invisible Ultrasmall Gold Nanoparticles-Framework for Synthesis
and Evaluation
TI - Innate Immune Invisible Ultrasmall Gold Nanoparticles-Framework for Synthesis
and Evaluation
VL - 13
ID - 6357
ER -
TY - JOUR
AB - Due to their antibacterial, antifungal, antiviral, and anti-inflammatory
properties, silver and, in recent years, nanosilver (NS) have been widely used in
various fields of human activity. However, it has been found that nanomaterials
acquire new properties, including those with respect to toxicity. The purpose of
this work was to study the effect of NS and the ionic form of silver, silver
sulfate (SS), on somatic mice cells in vivo. A model that is closest to the
conditions of exposure to humans, namely the supply of NS and SS with drinking
water, is used. The effect of NS particles coated with gum Arabic (diameter 14 ±
0.3 nm) and SS at concentrations of 0.1, 5, 50, and 500 mg/L upon 2-week exposure
is studied. A cytom assay, including counting the micronuclei and other nuclear
anomalies in the cells of the bone marrow, lung, colon, and bladder, was conducted.
No effect of NS or SS on bone-marrow cells is revealed in the standard micronucleus
test. NS at a concentration of 50 mg/L increases the cytogenetic effect by 1.9
times at the place of action, the colon, when compared to the control. In the
lungs, the rate of cells with micronuclei is increased threefold under the action
of NS at a concentration of 500 mg/L. The effect of NS on reducing the
proliferation level in the colon is confirmed in vivo; this effect has been
previously found in vitro by other authors. SS at a concentration of 50 mg/L
increases the rate of cells with cytogenetic lesions in the colon and bladder by
1.9 and 1.3 times, respectively. These effects should be considered when assessing
the risk of these compounds. © 2017, Pleiades Publishing, Ltd.
AU - Zhurkov, V. S.
AU - Savostikova, O. N.
AU - Yurchenko, V. V.
AU - Krivtsova, E. K.
AU - Kovalenko, M. A.
AU - Murav’eva, L. V.
AU - Alekseeva, A. V.
AU - Belyaeva, N. N.
AU - Mikhailova, R. I.
AU - Sycheva, L. P.
DB - Scopus
DO - 10.1134/S1995078017060143
IS - 11-12
KW - Bone
Cells
Cytology
Cytotoxicity
Mammals
Potable water
Risk assessment
Sulfur compounds
Anti-inflammatories
Bone marrow cells
Cytogenetic effects
Cytotoxic effects
Human activities
Ionic forms
Micronucleus test
Silver sulfate
Silver compounds
M3 - Article
PY - 2017
SP - 667-672
ST - Features of the Mutagenic and Cytotoxic Effects of Nanosilver and Silver
Sulfate in Mice
T2 - Nanotechnologies in Russia
TI - Features of the Mutagenic and Cytotoxic Effects of Nanosilver and Silver
Sulfate in Mice
85044779491&doi=10.1134%2fS1995078017060143&partnerID=40&md5=e169fd12bbbf96723a7535
10b45b8ccf
VL - 12
ID - 5544
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as
well as to agglomerate, which reduces the effectiveness of their use for the
antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake
structure, can be a carrier of AgNP that stabilizes their movement without
inhibiting their antibacterial properties. Considering that the human skin is often
the first contact with antimicrobial agent, the aim of the study was to assess
whether the application of the complex of AgNP and GO is biocompatible with the
skin model in in vitro studies. The conducted tests were performed in accordance
with the criteria set in OECD TG439. AgNP-GO complex did not influence the
genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the
pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon
gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well
as MIP1β (macrophage inflammatory protein 1β) expressed in the GO group. Moreover,
it showed a positive effect on the micro-and ultra-structure of the skin model. In
conclusion, the synergistic effect of AgNP and GO as a complex can activate the
process of epidermis renewal, which makes it suitable for use as a material for
skin contact. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
AU - Zielińska-Górska, M.
AU - Sawosz, E.
AU - Sosnowska, M.
AU - Hotowy, A.
AU - Grodzik, M.
AU - Górski, K.
AU - Strojny-Cieślak, B.
AU - Wierzbicki, M.
AU - Chwalibog, A.
C7 - 1398
DB - Scopus
IS - 7
KW - 3D epidermis
biocompatibility
cytokines
graphene oxide
morphology
interleukin 13
interleukin 1alpha
interleukin 1beta
interleukin 3
interleukin 4
RANTES
silver nanoparticle
Article
cell renewal
controlled study
epidermis
human
human tissue
hydrodynamics
in vitro study
particle size
pH measurement
protein expression
skin culture
ultrastructure
zeta potential
M3 - Article
PY - 2022
ST - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
Oxide to Human Skin in a 3D Epidermis In Vitro Model
T2 - Pharmaceutics
TI - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
85133728620&doi=10.3390%2fpharmaceutics14071398&partnerID=40&md5=648e0b851082342a5a
095630e361586a
VL - 14
ID - 5157
ER -
TY - JOUR
AB - Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as
well as to agglomerate, which reduces the effectiveness of their use for the
antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake
structure, can be a carrier of AgNP that stabilizes their movement without
inhibiting their antibacterial properties. Considering that the human skin is often
the first contact with antimicrobial agent, the aim of the study was to assess
whether the application of the complex of AgNP and GO is biocompatible with the
skin model in in vitro studies. The conducted tests were performed in accordance
with the criteria set in OECD TG439. AgNP-GO complex did not influence the
genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the
pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon
gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well
as MIP1 beta (macrophage inflammatory protein 1 beta) expressed in the GO group.
Moreover, it showed a positive effect on the micro- and ultra-structure of the skin
model. In conclusion, the synergistic effect of AgNP and GO as a complex can
activate the process of epidermis renewal, which makes it suitable for use as a
material for skin contact.
AN - WOS:000831693700001
AU - Zielinska-Gorska, M.
AU - Sawosz, E.
AU - Sosnowska, M.
AU - Hotowy, A.
AU - Grodzik, M.
AU - Gorski, K.
AU - Strojny-Cieslak, B.
AU - Wierzbicki, M.
AU - Chwalibog, A.
C7 - 1398
DA - JUL
IS - 7
PY - 2022
SN - 1999-4923
ST - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
T2 - PHARMACEUTICS
TI - Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene
VL - 14
ID - 6010
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been proposed as new alternatives to limit
bacterial dental plaque because of their antimicrobial activity. Novel glutathione-
stabilized silver nanoparticles (GSH-AgNPs) have proven powerful antibacterial
properties in food manufacturing processes. Therefore, this study aimed to evaluate
the potentiality of GSH-AgNPs for the prevention/treatment of oral infectious
diseases. First, the antimicrobial activity of GSH-AgNPs against three oral
pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus
mutans) was evaluated. Results demonstrated the efficiency of GSH-AgNPs in
inhibiting the growth of all bacteria, especially S. mutans (IC50 = 23.64 μg/mL, Ag
concentration). Second, GSH-AgNPs were assayed for their cytotoxicity (i.e., cell
viability) toward a human gingival fibroblast cell line (HGF-1), as an oral
epithelial model. Results indicated no toxic effects of GSH-AgNPs at low
concentrations (≥6.16 μg/mL, Ag concentration). Higher concentrations resulted in
losing cell viability, which followed the Ag accumulation in cells. Finally, the
inflammatory response in the HGF-1 cells after their exposure to GSH-AgNPs was
measured as the production of immune markers (interleukins 6 and 8 (IL-6 and IL-8)
and tumor necrosis factor-alpha (TNF-α)). GSH-AgNPs activates the inflammatory
response in human gingival fibroblasts, increasing the production of cytokines.
These findings provide new insights for the use of GSH-AgNPs in dental care and
encourage further studies for their application. © 2020 by the authors.
AU - Zorraquín-Peña, I.
AU - Cueva, C.
AU - de Llano, D. G.
AU - Bartolomé, B.
AU - Moreno-Arribas, M. V.
C7 - 375
DB - Scopus
DO - 10.3390/BIOMEDICINES8100375
IS - 10
Cytotoxicity
cytokines
Oral bacteria
Periodontal pathogens
M3 - Article
PY - 2020
ST - Glutathione-stabilized silver nanoparticles: Antibacterial activity against
periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
T2 - Biomedicines
TI - Glutathione-stabilized silver nanoparticles: Antibacterial activity against
periodontal bacteria, and cytotoxicity and inflammatory response in oral cells
85092586023&doi=10.3390%2fBIOMEDICINES8100375&partnerID=40&md5=66a01c350a759453eef9
b192ed0bf22c
VL - 8
ID - 5305
ER -
TY - JOUR
AB - Silver nanoparticles (AgNPs) have been proposed as new alternatives to limit
bacterial dental plaque because of their antimicrobial activity. Novel glutathione-
stabilized silver nanoparticles (GSH-AgNPs) have proven powerful antibacterial
properties in food manufacturing processes. Therefore, this study aimed to evaluate
the potentiality of GSH-AgNPs for the prevention/treatment of oral infectious
diseases. First, the antimicrobial activity of GSH-AgNPs against three oral
pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus
mutans) was evaluated. Results demonstrated the efficiency of GSH-AgNPs in
inhibiting the growth of all bacteria, especially S. mutans (IC50 = 23.64 mu g/mL,
Ag concentration). Second, GSH-AgNPs were assayed for their cytotoxicity (i.e.,
cell viability) toward a human gingival fibroblast cell line (HGF-1), as an oral
epithelial model. Results indicated no toxic effects of GSH-AgNPs at low
concentrations (<= 6.16 mu g/mL, Ag concentration). Higher concentrations resulted
in losing cell viability, which followed the Ag accumulation in cells. Finally, the
inflammatory response in the HGF-1 cells after their exposure to GSH-AgNPs was
measured as the production of immune markers (interleukins 6 and 8 (IL-6 and IL-8)
and tumor necrosis factor-alpha (TNF-alpha)). GSH-AgNPs activates the inflammatory
response in human gingival fibroblasts, increasing the production of cytokines.
These findings provide new insights for the use of GSH-AgNPs in dental care and
encourage further studies for their application.
AN - WOS:000584096500001
AU - Zorraquin-Pena, I.
AU - Cueva, C.
AU - de Llano, D. G.
AU - Bartolome, B.
AU - Moreno-Arribas, M. V.
C7 - 375
DA - OCT
IS - 10
PY - 2020
SN - 2227-9059
ST - Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity against
Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells
T2 - BIOMEDICINES
TI - Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity against
Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells
VL - 8
ID - 5859
ER -
TY - JOUR
AB - Liposome nanocarriers (LPNs) are potentially the future of inner ear therapy
due to their high drug loading capacity and efficient uptake in the inner ear after
a minimally invasive intratympanic administration. However, information on the
biocompatibility of LPNs in the inner ear is lacking. The aim of the present study
is to document the biocompatibility of LPNs in the inner ear after intratympanic
delivery. LPNs with or without gadolinium-tetra-azacyclo-dodecane-tetra-acetic acid
(Gd-DOTA) were delivered to the rats through transtympanic injection. The
distribution of the Gd-DOTA-containing LPNs in the middle and inner ear was tracked
in vivo using MRI. The function of the middle and inner ear barriers was evaluated
using gadolinium-enhanced MRI. The auditory function was measured using auditory
brainstem response (ABR). The potential inflammatory response was investigated by
analyzing glycosaminoglycan and hyaluronic acid secretion and CD44 and TLR2
expression in the inner ear. The potential apoptosis was analyzed using terminal
transferase (TdT) to label the free 3'OH breaks in the DNA strands of apoptotic
cells with TMR-dUTP (TUNEL staining). As a result, LPNs entered the inner ear
efficiently after transtympanic injection. The transtympanic injection of LPNs with
or without Gd-DOTA neither disrupted the function of the middle and inner ear
barriers nor caused hearing impairment in rats. The critical inflammatory
biological markers in the inner ear, including glycosaminoglycan and hyaluronic
acid secretion and CD44 and TLR2 expression, were not influenced by the
administration of LPNs. There was no significant cell death associated with the
administration of LPNs. The transtympanic injection of LPNs is safe for the inner
ear, and LPNs may be applied as a drug delivery matrix in the clinical therapy of
sensorineural hearing loss.
AN - WOS:000402198200003
AU - Zou, J.
AU - Feng, H.
AU - Sood, R.
AU - Kinnunen, P. K. J.
AU - Pyykko, I.
C7 - 372
DA - MAY 25
DO - 10.1186/s11671-017-2142-5
PY - 2017
SN - 1556-276X
ST - Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After
Intratympanic Administration
TI - Biocompatibility of Liposome Nanocarriers in the Rat Inner Ear After
Intratympanic Administration
VL - 12
ID - 6402
ER -
TY - JOUR
AB - Asthma is a chronic airway inflammatory disease with complex mechanisms, and
these patients often encounter difficulties in their treatment course due to the
heterogeneity of the disease. Currently, clinical treatments for asthma are mainly
based on glucocorticoid-based combination drug therapy; however, glucocorticoid
resistance and multiple side effects, as well as the occurrence of poor drug
delivery, require the development of more promising treatments. Nanotechnology is
an emerging technology that has been extensively researched in the medical field.
Several studies have shown that drug delivery systems could significantly improve
the targeting, reduce toxicity and improve the bioavailability of drugs. The use of
multiple nanoparticle delivery strategies could improve the therapeutic efficacy of
drugs compared to traditional delivery methods. Herein, the authors presented the
mechanisms of asthma development and current therapeutic methods. Furthermore, the
design and synthesis of different types of nanomaterials and micromaterials for
asthma therapy are reviewed, including polymetric nanomaterials, solid lipid
nanomaterials, cell membranes-based nanomaterials, and metal nanomaterials.
Finally, the challenges and future perspectives of these nanomaterials are
discussed to provide guidance for further research directions and hopefully promote
the clinical application of nanotherapeutics in asthma treatment.
AN - WOS:000887581000001
AU - Zuo, X.
AU - Guo, X. P.
AU - Gu, Y. N.
AU - Zheng, H. Y.
AU - Zhou, Z. J.
AU - Wang, X. L.
AU - Jiang, S. Y.
AU - Wang, G. Q.
AU - Xu, C. N.
AU - Wang, F.
C7 - 14427
DA - NOV
DO - 10.3390/ijms232214427
IS - 22
PY - 2022
SN - 1422-0067
ST - Recent Advances in Nanomaterials for Asthma Treatment
TI - Recent Advances in Nanomaterials for Asthma Treatment
VL - 23
ID - 6810
ER -

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AB - The proceedings contain 2 papers. The topics discussed include: carbonaceous

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