Members: Group 2 Schedule: 8073 Score:

Bacagan, Claydee Joy 1:30-4:30PM ThFS

Faustino, Ivan Kara B. Date:
Mabalot, Erika July 21, 2022
Salongkit, Jeremiah
Laboratory Experiment no. 10
Clot Retraction Test
1. Describe other methods for clot retraction.
Hirsboek and Coffey method: used test-tubes 1 cm in diameter. They added 2 ccs of blood to
the tubes, which were closed with a rubber stopper and shaken to create a certain amount foam.
The coagulation time was first estimated by inverting the tubes, and after clotting they were left
at room temperature. The time of the first sign of retraction of the clots from the sides of the
tubes was taken as the retraction time. (Contributor: Mabalot, E.)
Macfarlane method: The tube was kept at 37°c~ for one hour; the glass rod with the adherent
clot was lifted out, and the serum measured on the scale of the tubes. (Contributor: Faustino, I.)

2. Give diseases or conditions which yield an abnormal clot retraction test.

Clot retraction can also be affected by disturbances in the coagulation system, which lead to
reduced thrombin formation and thus reduced platelet activation. Accordingly, many coagulation
factor deficiencies including factor VII, VIII, XI, XIII as well as fibrinogen lead to a decrease in
clot retraction. (Contributor: Bacagan, C.)
In systemic lupus erythematosus, another inflammatory disease, clot retraction is decreased.
Glanzmann thrombasthenia, leads to abolished clot retraction due to an inborn defect of the
αIIbβ3 receptor. (Contributor: Faustino, I)

3. Whole blood that has coagulated typically retracts from the sides of the container,
resulting in the separation of transparent serum and formation of a blood clot. What
particular component is attributed to this phenomenon? How does this happen?
When platelets are adequate in number and function is normal, the blood will form a clot in the
collection tube and retract away from the sides of the tube. Plasma clots retract away from the
sides of a glass tube within a few hours allowing the rapid characterization of outside-in signaling
through platelet integrin αIIbβ3. Clot retraction normally takes between 1-2 hours. (Contributor:
Salongkit, J.)

Reference:
Hirschboeck, J. S., & Coffey Jr, W. L. (1942). Method for Measuring Clot Retraction Time. Arch.
Path., 33(March), 380.
Jansen, E. E., & Hartmann, M. (2021). Clot Retraction: Cellular Mechanisms and Inhibitors,
Measuring Methods, and Clinical Implications. Biomedicines, 9(8), 1064.
https://doi.org/10.3390/biomedicines9081064
Macfarlane, R. G. (1939). 'A simple method for measuring clot retraction Lancet, i^ 1199.