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Abstract
Fourier Transform Infra Red (FTIR) Spectroscopy is a non-invasive, reagent free diagnostic tool in the analysis of
biological fluids. The results can be best employed in the qualitative and quantitative investigation of biological
fluids. Multiple myeloma is a disorder in which malignant plasma cells accumulate in bone marrow and produce
excess immunoglobulin that leads to many complications. The present work is attempted in the study of normal
and three different myeloma affected blood samples IgA, IgG and IgM using FTIR Spectroscopy. Internal
Standard method is adopted in characterizing the samples quantitatively.
Keywords: Immunoglobulins; Multiple Myeloma; Non-invasive; Reagent free diagnostic tool; Fourier Transform
Infrared Spectroscopy.
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Research Article Biology and Medicine, 2 (3): 42-48, 2010
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Research Article Biology and Medicine, 2 (3): 42-48, 2010
1
deformation of methyl and methylene group of The spectral region 1250-925 cm- is
the proteins. The asymmetric and symmetric predominantly occupied by C–O–C
P-O stretching vibrations are found to be asymmetric and symmetric vibrations of
around 1245 cm-1 and 956 cm-1 respectively. phospholipids of proteins (Randhawa, 2003).
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Research Article Biology and Medicine, 2 (3): 42-48, 2010
The experiment is repeated in other shows the absorption levels of the various
types of myeloma samples, IgA and IgM peaks are increased in IgA sample than that of
respectively. Figure 3 presents FTIR spectra normal sample. Table 1 verifies the same
of normal and IgA blood samples. The figure absorption by intensity ratio calculation.
Fig 4 represents the FTIR spectral 1 presents intensity ratio calculation for all the
analysis of normal and IgM blood samples. It three types of myeloma samples in the same
is observed that spectral absorption of the spectral regions as a comparison. Fig 5
peaks are decreased in IgM samples and presents variations as a bar diagram.
hence the intensity ratio calculation also. Table
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Research Article Biology and Medicine, 2 (3): 42-48, 2010
0.8
0.7
0.6
0.5
Normal
Ig G
0.4
Ig A
Ig M
0.3
0.2
0.1
0
R1 R2 R3 R4 R5
Figure 5: It shows the comparison of Intensity Ratio Parameters between Normal and Myeloma
Samples.
Table 1: It shows the comparison of Normal and three different myeloma samples using Intensity
Ratio Parameters.
Myeloma Myeloma Myeloma
Ratios Normal Serum Ig G Ig A Ig M
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Research Article Biology and Medicine, 2 (3): 42-48, 2010
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