HumaStar 200

Complement C4
[REF] 11113 Material required but not provided:
[REF] 11117 C3/C4/TRF Standard
[REF] 13010 Turbidos
For detailed information about reagents [REF] 18973 Cuvette Clean ***
please refer to our corresponding leaflet

Test Name Complement C4 9 - Pathological ranges

Code C4 #
Type Endpoint
Group Turbi 10 - Result units
Units 1: mg/dl
1 - Pipetting 1st 2nd Units 2:
Sample vol: 10 µl Conversion: 0
Reagent 1 vol: 250 µl Decimal digits: 1
Reagent 2 vol:
Diluent vol: 0 µl 11 - Calibrator factor limits
Min factor: 0
2 - Time Max factor: 0
Incubation 1: 324 sec
Incubation 2:
Reading: 36 sec Calibration

3 - Wavelengths Number of standards: 5 *

Wavelength 1: 340 nm repetitions: 1
Wavelength 2: Factor: cr
Validity (hours): 120
4 - Washing Interpolation type: Four Parameters
Needle: 3
Cuvette: 6 Calibrator: C3/C4/TRF Standard (!) mg/dl
alternative Calibrator:
5 - Incompatibility Dilution rate: 16, 8, 4, 2, 1
1
2 Blank correction: Yes
3 Validity (hours): 12
4
Reagents:
6 - Limits R1: [RGT1]
Blank OD min: -0.1 Abs
Blank OD max: 0.2 Abs
Reaction slope positive C-Clean: sample position 60
OD Range min: -0.1 Abs cr calculated by run
OD Range max: 1.0 Abs # User defined
Min conc: 6.0 mg/dl * employed
Max conc: 120.0 mg/dl (with autodilution)

*** For instructions how to use, see user manual.

(!) Standard 0 is not used.
Application sheet TU-C4-200-2014-08-001 Rev. 2

7 - Autodilution NOTE:
Rate: 2
The settings in the software of your HUMAN analyzer should represent the latest version. If there
Max OD: 0.8 Abs are differences between the settings in this application sheet and those in your analyzer software,
kindly contact the Customer Service of HUMAN: customer-support@human.de before changing
the analyzer settings according to this application sheet. The laboratory has to validate the results
8 - Dilutions of this method in its own responsibility.
Serum 1:1
Urine 1:1

Date: Version:
Human Gesellschaft für Biochemica und Diagnostica mbH
TU-C4-200 2014-08 001
Max-Planck-Ring 21 - D-65205 Wiesbaden - Germany
Tel.: +496122 99880 Fax: +496122 9988100 - e-mail: human@human.de