ABSTRACT

ISOLATION AND IDENTIFICATION OF ENDOPHYTIC FUNGI IN SEVERAL

VARIETIES OF COFFEE PLANTS (Coffea sp.) ORIGIN OF BAJAWA, EAST NUSA
TENGGARA.

By: Maria Febriana Mite

Under Supervision Of :
Prof. Ir. Yosep Seran Mau, M.Sc., Ph.D and Prof. Ir. Agnes V. Simamora, MCP., Ph.D
Interest in Plant Protection, Department / Study Program of Agrotechnology, Faculty of
Agriculture, Nusa Cendana University.

One type of biological control agent is endophytic fungi. Endophytic fungi are fungi that
live on plant tissues such as leaves, flowers, fruits or plant roots but do not harm the plant.
Endophytic fungi are found in almost all plants, one of which is on coffee plants. This study aims
to determine the types of endophytic fungi found in the tissues of coffee plants (Coffea sp.)
Arabica, Robusta, Yellow caturra and Kartika varieties that have the potential to be biological
control agents. In this study, an exploration of endophytic fungi on leaves, fruits and twigs of
coffee plants was carried out. Coffee plant samples were taken from coffee fields owned by
farmers in Bajawa, East Nusa Tenggara and reviewed by research at the UPT Bioscience
Laboratory of Nusa Cendana University from March to June 2023. From this study, 33 isolates
of endophytic fungi consisting of 19 genera were obtained, namely: Trichoderma, Penicillium,
Fusarium, Chepalosporium, Papulaspora, Cladosporium, Colletotrichum, Gliocladium,
Paecilomyces, Nigrospora, Aspergillus, Helicocephalum, Mucor, Cladophialophora,
Phialophora, Phoma, Septonema, Hansfordia and Aureobasidium.

Keywords: Coffee Plant, Endophytic fungi, Exploration.