QUALITY CONTROL OF ANTI SERA

ANTI A ANTI B ANTI D AHG ALB22%

COLOUR

TURBIDITY

ANTI A ANTI A ANTI B ANTI B ANTI D ANTI D AHG (POLY) ALB (22%)

REAGENT SPECIFICITY + + + + + + + +
A
B B A O POS O NEG CHECK CELLS O POS CELLS
CELLS
CELLS CELLS CELLS CELLS CELLS

PHASE IS IS IS IS IS IS IS IS

REACTION

RESULT

DATE: SIGNATURE:
DATE REAGENTS EXPIRY APPEARANCE RESULT
DATE

COLOUR TURBIDITY SEAL

BROKEN
 Procedure of Anti HIV 1+2 ELISA

1. Mark four wells 2 positive control, 1 negative control and

1 sample.
2. Add 100 µL of positive control, negative control, and
SAMPLE into their respective wells. Mix by taping the
plate gently.
3. Cover the plate and incubate for 30 minutes at 37C.
4. Wash each well 5 times with diluted washing buffer and
soak for 30sec.
5. Add 100 µL of HRP conjugate into each wells and mix by
taping the plate gently.
6. Cover the plate and incubate for 30 minutes at 37C.
7. After the final washing cycle turn down the plate onto
blotting paper or clean towel and tap it to remove
remainders.
8. Add 50µl of chromogen A and 50µl of chromogen B
solution into each wells
9. Incubate the plate at 37C for 15 minutes. After that blue
color shows positive control.
10. Add 50µl stop solution into each wells mix gently
intensive yellow color develops in positive control.
11. Read the absorbance with in 10minutes after stopping the
reaction.
Unit Return policy:

PURPOSE:

Due to any reason, doctor/ staff nurse can return unit to blood bank.

Only Red Cells product is acceptable to receive in blood bank to re-

store / or for re-use with following condition,

Unit must be return within 30 minutes of issue

Temperature must be within 100C.

All seals and ports must be intact.

Other than red cell all products including Platelet, FFP,

Cryosupernatent and Cryoprecipitate etc. are not acceptable to receive

in blood bank to store / or for re-use, if returns product(s) will be

discarded.

Hemolysis and discoloration is not observed on visual inspection of

the bag.

Reason of return should be documented on register by MO.

SCOPE:

This will applicable to Indus lab

Verify time of unit issue, if it is within 30 minutes proceed for return

Note:

For Ward:If time of issue is more than 30 minutes, inform

patient’s attending doctor / staff that product is going to discard.

Make sure that product is received in transport container.

Note: If unit brought to blood bank without container, it must be

discarded.

Inspect unit and make sure seals and ports are intact.

Note: If seal/port is open or transfusion set is inserted, consider it as

open seal/port and discard the unit.

 Unit issue :

PURPOSE

Unit issue is an important step in transfusion practices. Purpose of this module is to Issue

right product to the right patient thus prevent them from any adverse reaction to

transfusion

 Take the patients vitals especially temperature before sending ward boy to pick up

blood.

 Complete information should be sent to the blood bank by ward staff (patients

name, MR no. on paper signed by mo or nursing staff at the time of issue.

 In case any discrepancy in the name of patient MR no. or incomplete information

unit will not be issued.

 Blood bag should be issue only in transport box.

Unit from outside:

Blood unit drawn and processed outside the Indus blood bank is not
allowed this shell be allowed only in case of life saving measure and non
availability in the inventory.
If any unit is saved from out side Indus blood bank will not be
responsible for any adverse reaction or diease transmission.
Transfusion reaction :

 in case of any transfusion reaction immediately stop the

transfusion and informed the blood bank.
 Send the blood bag along with post transfusion samples and urine
smple to the blood bank, with the transfusion reaction form
completely filled by the duty doctor.
 Follow the recommendation givin by the blood bank in case of any
transfusion reaction.

Unit discard
Purpose:
To eliminate any sub standard product (expired, broken frozen
product, or unit showing leakage e.t.c,
 When empty blood bag received by lab staff must be
documented in unit discard register along with complete
information.
 Emapty bag should be crossed and cut in between and dip
this bag in 10% bleach and put this bag in yellow bin (A.T
waste)
 Expired blood bag should be drain in sanitary sewar. The
drain should then be immediately flushed with water,
followed by 250ml of 10% hypochlorite and finally again
flushed with water.