PG Biochemistry Syllabus

ROYAL SCHOOL OF BIO - SCIENCES
(RSBSC)
Department of Biochemistry
Learning Outcomes based Biochemistry Postgraduate

Programme Curriculum Framework (LOCF)
For
M.Sc Biochemistry (Honours)
1
Preamble-
The following points were taken into consideration while making the syllabus for Post Graduation in
Biochemistry in accordance with the UGC guidelines of Learning Outcome Based Curriculum
Framework.
1. The learning outcomes are designed to help life science graduates to understand theobjectives of
studying M.Sc.(Hons.) Biochemistry that is, to analyse, appreciate, understand the basic concepts
of chemical reactions that occur in living systems, which enable them to understandthe various
perspectives of applied sciences that benefit themankind.
2. The organization of the courses/papers have been worked into semesters while keeping in
consideration the credit load in each semester with the ultimateend of outcomes of
thecourse/programme.
3. Learning outcomes can be modified later with proper justification in response tosituational
environments globally or as per regionalrequirements.
Part I
Introduction
The discipline of Biochemistry is regarded as Mother of all Biological Sciences disciplines because it
unveils the chemical basis of life in all living organisms including plants, animals and
microorganisms.Biochemistry has enourmously contributed in improving the understanding of many of
the metabolic processes all across different forms of life. This has helped in achieving massive
improvements in the field of health sciences, agriculture and its many branches, drug designing, food
technology, and also in environmental bioremediation.
Keeping in pace with the developmental trends in various subareas of Biochemistry it is expected that the
students undertaking Biochemistry (Honours) course at postgraduate level become conversant with the
fundamentals of Biochemistry and at the same time at the end of the programme they exhibit certain
levels of learning outcomes. Such learning outcomes like
understandingofdiscipline,criticalthinking,problemsolving,analyticalandscientificreasoning,
research/industry related skills, etc. will empower the students to develop their future career with
amuchbetterandmeaningfulorientation.WiththisbackgroundLOCF-Biochemistrypostgraduate model
curriculum has been developed, which includes 10 core theory papers, 12 department
specifictheorypapersandcorrespondingpracticalpapers.Thecoursecontentsinclude fundamentals as well as
upcoming developments in the discipline of Biochemistry and interfacial sciences.
Learning Outcomes-based Approach to CurricularPlanning
In the learning outcome-based approach, extensive deliberation has been made to identify the
minimumlearningoutcomefromastudentaftercompletingeachcourse. To achieve better understanding parallel
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practical components have been introduced. In this regard the department has been maintaining a healthy
student ‗teacher ratio for both theory and practical classes.
Nature and extent of the M.Sc. (Hons.)Programme:
The course is designed as per the UGC regulation for a period of two years where the students have to
study 10 Core courses in Biochemistry, 12 advance courses in Biochemistry known as Discipline Specific
Elective Courses, and 2 Ability Enhancement Compulsory Courses. The final two can be selected by the
students depending on their choice in other departments as per the courses available in other departments
of the university. In first three semesters the students are provided basics of Biochemistry syllabus,
besides the courses which they have to opt in other departments.
Aims of Master’s degree (Honours) programme inBiochemistry:
The overall aims of Master‘s degree (Hons) programme in Biochemistry are to:
1. Provide students with a platform to develop their interest in learning biochemistry; develop in-depth
understanding of the working of biomolecules, key biochemical concepts, and equip students with
appropriate technical and analytical skills to tackle issues and problems in the field of biochemistry.
2. Expose the students to a wide range of careers that combine biology, plants,and medicine.
3. Providestudentswithsomework/researchexperience,viaaresearch-baseddissertationworkin a research
laboratory to further boost the careerprospects.
3. Provide students with the knowledge and skill base that would enable them to undertake further studies
in biochemistry and related areas and help develop a range of generic skills that are relevant to the
needs of the biotech/pharma industry or the major research institutions, or for entrepreneurship.
Qualification descriptors for a Master’s degree inBiochemistry:
The qualification descriptors for the M.Sc. programme in Biochemistry shall be five learning attributes
such as understanding, use, communication, and demonstration of experimental and theoretical
knowledge with a clear understanding. The key qualification descriptor for postgraduate Biochemistry
shall be clarity of concepts, experimentation, communication as well as critical thinking and ethical
awareness. Each postgraduate in Biochemistry should be able to
• demonstrate a coherent and systematic approach to the experimental and theoretical aspects of
biochemistry.
• demonstrate the ability to understand the role of scientific developments, particularly,biological sciences
in a changing world from the disciplinary perspective as well as in relation to its professional and
everyday use.
• communicate ideas, opinions, and values—both scientific themes and values of life in all shades and
shapes—to expand the knowledge of the subject as it moves from the classroom/laboratory to industry
andsociety.
• demonstratetheabilitytosharetheresultsofacademicanddisciplinarylearningthroughdifferent forms of
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communication such as essays, dissertations, reports, findings, notes, seminars, research papers etc, on
different platforms of communication such as the classroom, the media, and the internet.
• recognize the scope of biochemistry in terms of career opportunities, employment and lifelong
engagement in teaching, publishing, communication, media, soft skills, other allied fields or engaging in
research activity that can benefit the society regionally, nationally, orglobally.
Theprogrammewillstrengthenthestudent‘s ability to comprehend

currentsciencethinkofwaystofindlogicalandviablesolutions. The
qualificationdescriptorsfortheM.Sc.(Biochemistry)programmeshallthusincludeunderstanding of
fundamentals, acquiring practical training and application ofthe subject knowledge in diversified areas of
Biochemistry with a clear understanding that this knowledge will equip the students to make them
suitable for various Biotech, Pharma, Medicine, Agri-Biotech, Biochemical related
laboratories/industries. The key qualification descriptor for
BiochemistryshallbeacquiringpracticaltrainingaswellascriticalknowledgeoftheBiochemistry subject.
Programme Learning Outcomes (M.Sc. Hons. inBiochemistry)
The learners who complete two years of full time postgraduate (Honours) programme in Biochemistry
would earn a Master‘s (Honours) degree in Biochemistry. The learning outcomes that a student should be
able to demonstrate on completion of a degree level programme may involve academic, personal, and
behavioral as well as entrepreneurial and social competencies. These outcomes will provide insight to the
faculty members teaching the course. This is the most critical part and therefore it is essential that faculty
members undergo rigorous orientation
programmepriortoteachingthecourseTheprogrammelearningoutcomes relating to M.Sc.(Honours) degree
programme in Biochemistry can be summarized as under:
1. AcademicCompetence
2. Personal and BehaviouralCompetence
3. Entrepreneurial and socialcompetence
Teaching learningprocess
In order to make learning an enjoyable activity, learners should be encouraged to engage in a rigorous
process of learning and self-discovery by adopting a application based, highly focused and yet flexible
approach to education as opposed to rote learning. In teaching and learning pedagogy, there will be a shift
from domain or conclusions-based approach to the experiential or process/es-based approach.
The teaching of postgraduate biochemistry for each course, except ability enhancement ability
courses,shallincludelecturesfollowedbyLaboratoryexerciseforthatcourse.Lectures will have good
proportion of visuals learning component and ICT enabled delivery.
4
ForoverallunderstandingofthesubjectBiochemistry,multipleteaching tools can beused.
Lectures
ICT tools involving smart boards, power point presentations, live demonstrations, videos, animations,
models, improve the understanding of basic molecular interactions in the living systems and make the
teaching sessions enjoyable. Lectures will be interactive in a way that students work with their teachers to
get new insights in the subject area.
Discussions
To develop critical thinking, reasoning, interactive

skillsinstudentstheteachingsessionsshouldbemademoreinteractive.Tutorialclassesarebeing frequently
organized as it will promote more interaction of the students with teachers.
Practical
Aftercompletionofexperimentsinpracticalclass,
studentsshouldbegivenrelatedproblems.Thiswillenhancetheabilityofproblem-basedlearning (PBL).
Case Studies:
Toexpressacquiredknowledge,skillsandattitudes,case-basedlearning(CBL)canbeusedwhere students can be

given case specific problems both for theory and practical courses to findcreative solutions to complex
problems of individual, community, society and various aspects of knowledge domain in the concerned
areas of lifesciences.
Project work
The students will be encouraged to carry out small project work of their choice to quench their curiosity.
To understand research, student can undertake a small dissertation work where
he/sheexhaustivelyperformstheliteraturesearchandcompilesthemasameaningfulpresentation.
Teamwork
Positivecollaborationintheformofteamworkiscriticalintheclassroomenvironment,forwhich it is necessary to
transcend one‘s prejudices and predilections to achieve the desired outcomes. In the process of teamwork,
learners will acquire the skills of managing knowledge
acquisitionandothercollaborativelearners,therebyunderstandinghowtoincorporateandbalance personalities.
Study Tours/FieldVisits
Study Tours/field trips provide opportunities to the students to explore further their theory and practical
5
learning of Biochemistry. These may include visits to Premier Research Institutes and Industrial
establishments.AlistofsuchResearch/Industrialinstitutionshasbeenappendedattheendofthis curriculum.
ASSESSMENTMETHODS
The assessment of learners‘ achievement in M.Sc.(Hons.) Biochemistry will be aligned withthe

following:
1. programme learning outcomes (graduatedescriptors)
2. course learning outcomes (qualificationdescriptors)
3. academic and professional skills suggested in the graduate learning descriptors in the LOCF
recommendations (indicated and illustrated in the Learning Outcomes in respectof selectcourses)
Assessment priorities: University will prioritize formative assessments (in-semester activities

including tests done at the department or instructor level) rather than giving heavy and final weightage to
summative assessments (end-semester and/or mid-semester tests traditionally
donecentrally).Progressoflearnerstowardsachievinglearningoutcomesmaybeassessedmaking creative use
of the following, either independently or in combination: time-constrained examinations (say 1-hour or 2-
hour tests); closed-book and open-book tests (if applicable, rather than doing as a rule); problem based
assignments; real life simulations; observation of practical skills (speaking, listening, problem solving
within a peer group or a class); individual project reports (case-study or term papers within a given word
limit); team project reports; oral presentations, including seminar presentation; viva voce, interviews;
computerized adaptive testing for MCQ; peer and self-assessment etc. and any other pedagogic
approaches as may be relevant keeping in view the learners‘ level, credit load and class size.
Diversity in Assessment Methods: Allowing for the diversity in learning andpedagogical methods
are expected to ensure that the objectives of the course(s) are clearly aligned to learning outcomes. The
curricula developed has been made with a transparent roadmap of (a) pedagogical methods and priorities
and (b) learning outcomes that reflect the weightage points given to different aspects of skills and
achievements identified in therecommendations.
Keywords
Biochemistry, courses, practicals, metabolism, enzymes, genes, carbohydrates, proteins, lipids, DNA,
RNA, vitamins, amino acids, physiology, respiration, fermentation, molecular biology, nutrition,
microbiology, immunology, synthetic biology, metabolic engineering, genomics, proteomics,
recombinant DNA, genetic engineering, history.
Part-II
Structure of M.Sc. (Honours) Biochemistry curriculum
To meet the objectives of postgraduate program in Biochemistry Core (Foundational) Courses, Elective
6
Courses (Specializations from within Biochemistry and from allied disciplines), Ability Enhancement
Elective Courses are envisaged. The programme includes a variety of courses with different credits
comprising of Core Courses (C) and elective courses. Core courses are all mandatory courses, Discipline-
Specific Elective (DSE) can be chosen. Besides, the programme also includes two compulsory courses
termed as Ability Enhancement Compulsory (AEEC) courses. The Core courses (CC) also termed as
Foundational Courses are designed to promote common educational edifice for the discipline of
Biochemistry.
ThesecourseswillprovidefoundationalknowledgeofBiochemistryanditsvarioussubdisciplines to the
students. Each Core Course in Biochemistry (BCH C) will be of comprising off theory and two
creditspractical.
The ―Discipline-Specific Elective (DSE)‖ courses are specialized or advanced courses and are intended to
cater to various specializations within Biochemistry broader discipline according to
CBCSprogramrequirements.Thesecoursesofferchoicesandcanbeoptedfromapoolofcourses.
These courses provide extensive exposure in the area chosen. Each Discipline Specific Elective Course in
Biochemistry (BCH DSE) will be of three/four credits theory.
Ability enhancement compulsory courses will include Communicative English and Behavorial Sciences
of 1 credit each per semester in the first two semesters. Communicative English will be continued
throughout the course and in the final two semesters there will be skill-based ability enhancement courses
with 2 credits per semester each which the students can select from the various courses being offered by
the university.
To complete M.Sc.(Hons.) Biochemistry programme, a student must study ten Core Courses (BCH C),
eight Discipline-Specific Electives (BCH DSE), four practical courses, two Skill Enhancement Elective
Courses (BCH SEE) and four compulsory Ability Enhancement Elective (BCH AEE) Courses. The Core
Courses, Discipline-Specific Electives and practicals are three/four-credit courses, whereas Skill
Enhancement Courses and Ability Enhancement Courses are 4 credit courses. A student must earn 102
credits to obtain M.Sc. (Honours) Biochemistry degree.
The curriculum proposes to have a strong interface between regular and online learning modes,
thereby,activelyutilizingnationalknowledgenetworkresources.Thefacultywouldutilizevirtual and actual
classrooms through video lectures, small group discussions, tutorials, and individual presentations.
Community engagement may be incorporated through socially driven small scale research projects,
workshops, field/laboratory training/experiential exercises and simulations utilizing currently available
knowledge systems and technological facilities. Care has been taken to bring in the elements of skill to
enhance employmentopportunities.
Lecture (L): One hour of teaching per week in a semester is 1 credit

Practical (P): Two hours of practical per week in a semester is 1credit
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TABLE OF CONTENTS
SL. NO. CONTENTS PAGE NOS
1 SCHEME FOR EVALUATION 9
2 PROGRAMME STRUCTURE 10-12
3 SYLLABUS FOR 1ST SEMESTER 14-25
4 SYLLABUS FOR 2ND SEMESTER 29-37
5 SYLLABUS FOR 3RD SEMESTER 39-46
6 SYLLABUS FOR 4TH SEMESTER 48-72
7 DISCLIPLINE SPECIFIC ELECTIVE 74-102
8
SCHEME OF EVALUATION
I. Theory Papers (T): II. Practical Papers (P): III. Combined Theory &
Practical Papers (TP):
Continuous Evaluation: 15% Continuous Evaluation: 25% Continuous Evaluation: 15%

(Assignment, Class Test, (Skill Test, lab copy, viva, lab (Assignment. Class Test,
Seminar, Quiz : Any Three) involvement: Any Three) Seminar, Lab Experiment,
Mid-term examination: 10% Attendance: 5% Copy and Viva: Any Three)
Attendance: 5% End term examination: 70 % Mid-term examination: 10%
End Term Examination: 70% Attendance: 5%
End term examination: 70 %
9
SCHEME AND COURSE STRUCTURE FOR CHOICE BASED CREDIT
SYSTEM IN
M.Sc. HONOURS BIOCHEMISTRY
1st semester 2nd semester
S Subject Names of L T P C TC S Subject Names L T P C T

l. Code subjects P l. Code of CP
N N subjects
o. o
.
Core Subjects (please use rows as required) Core Subjects (please use rows as required)
1 BCH154 Chemistry 3 1 0 4 4 1 BCH154 Enzymolo 3 1 0 4 4

C101 of C201 gy
Biomolecul
es
2 BCH154 Cell 3 1 0 4 4 2 BCH154 Molecula 3 1 0 4 4

C102 Biology and C202 r Biology
Physiology
3 BCH154 Bioenerget 3 1 0 4 4 3 BCH154 Immunol 3 1 0 4 4

C103 ics and C203 ogy
Metabolism
4 BCH154 Practical I 0 0 3 3 6 4 BCH154 Practica 0 0 3 3 6

C114 C214 l II
Ability Enhancement Compulsory Course Ability Enhancement Compulsory Course

(AECC)* (AECC)*
5 CEN984 Communic 1 1 5 CEN984 Communi 1 1

A101 ative A201 cative
English – I English – II
6 BHS984 Behaviour 1 1 6 BHS984 Behaviou 1 1

A103 al Science - A203 r al
I Science -
II
10
Ability Enhancement Elective Course (AEEC)
(Skill Based):
7 AEEC/SE 2 2
C/-1*
Elective: Discipline Specific DSE Elective: Discipline Specific DSE
7 BCH154 DSE – 1 3 1 4 4 8 BCH154 DSE – 2 3 3 3

D101 D201
Total:21 Total:22
3rd semester 4th semester
S Subject Names of L T P C TC S Subject Names L T P C T

l. Code subjects P l. Code of CP
N N subjects
o. o
.
Core Subjects (please use rows as required) Core Subjects (please use rows as required)
1 BCH154 Adavnced 2 1 0 3 3 1 BCH154 Microbiol 2 1 0 3 3

C301 Enzymolog C401 ogy
y
2 BCH154 Clinical 2 1 0 3 3 2 BCH154 Membran 2 1 0 3 3

C302 Biochemist C402 e Biology
ry
3 BCH154 Practical 0 0 3 3 6 3 BCH154 Practica 0 0 3 3 6

C313 III C413 l IV
Ability Enhancement Compulsory Course Ability Enhancement Compulsory Course

(AECC)* (AECC)*
4 CEN984 Comm. 1 1 4 CEN984 Comm 1 1

A301 Eng. A401 . Eng..
Ability Enhancement Elective Course (AEEC)

(Skill Based):
5 AEEC/SE 2 2
C/-2*
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Elective: Discipline Specific DSE Elective: Discipline Specific DSE
6 BCH154 DSE – 3 3 1 4 4 5 BCH154 DSE - 6 3 1 4 4

D301 D401
7 BCH154 DSE – 4 3 1 4 4 6 BCH154 DSE - 7 3 1 4 4

D302 D402
8 BCH154 DSE – 5 3 1 4 4 7 BCH154 DSE - 8 3 1 4 4

D303 D403
Project Dissertation Project Dissertation
9 BCH154 Minor 5 10 9 BCH154 Major 9 18

C324 C424
Total:29 Total:31
DISCIPLINE SPECIFIC ELECTIVE
SEMESTER 1ST SEMESTER 2ND SEMESTER III SEMESTER VI
Anyone to be selected Any one to be selected Any three to be selected Any three to be selected
Bioanalytical technique Nutritional Environmental Science Biochemistryof

Biochemistry Common Disorders
Biostatistics and Neurobiochemistry Research methodology Genetic engineering
bioinformatics
Genetics for biologics General Pharmacology
Microbial Biochemistry Biomaterials
12
1ST SEMESTER SYLLABUS
13
PAPER I: CHEMISTRY OF BIOMOLECULES
SUBJECT CODE: BCH154C101
CREDIT UNITS: L-T-P-C = 3-1-0-4
STUDENT’S SCHEME OF EVALUATION: Theory Papers (T)
Course Objective:
 The course aims to provide students with an understanding of the biomolecules the basic building blocks of
living organisms, focusing on their structural underpinnings, unique properties, biological roles and functions
and inter relations.
 Emphasis will be on the association between structure and function of various biomolecules at a
chemical level with a biological perspective as well as hands on approach and laboratory
techniques.
 Students will be introduced to bioinformatics and its application.
Modules Course content Periods

Stereochemistry,chirality, RS designation; chemistry and properties of water; pH and
indicators; acid-base concept; mono- di- and poly- protic acids; buffer solutions and their
I action; reactive reaction intermediates (carbocation, carbanion, carbene carbyne, and free 16
radicals); dielectric constant; surface tension; viscosity; dipole moment.
Structure, properties, and classification of amino acids; titration curves; peptide bond &
Ramachandran plot; levels of protein structure; globular, membrane & fibrous proteins;
interactions stabilizing proteins; supersecondary& domain structure; protein targeting;
II protein folding, misfolding & aggregation; molecular chaperones; unstructured proteins; 16
protein dynamics; naturally occurring peptides.
Structure and properties of various forms of DNA and RNA; forces stabilizing nucleic acid
structures; structure, chemistry and biological functions of carbohydrates and lipids;
III glycoproteins & lipoproteins. 16
Introduction to bioinformatics & computational biology; applications of bioinformatics;

biological databases; tools in bioinformatics (sequence retrieval, global & local alignment,
phylogenetic analysis, pathway analysis, protein structure retrieval & visualization); basics
IV of protein modeling and molecular docking. 16
Total 64
Text Books:
1. Branden, C.I. and Tooze, J. (2009) Introduction to Protein Structure. Second edition, CRC Press.
14
2. Finkelstein, A.V. and Ptitsyn, O.B. (2016) Protein Physics: A course of lectures. Second edition, Academic
Press Publications.
3. Almeida, P. (2016) Proteins Concepts in Biochemistry. Garland Science Publishers.
4. Nelson D.L. and Cox M.M. (2017) Lehninger's Principles of Biochemistry, Seventh Edition, Freeman &
Co, New York.
5. Atkins P.W. (2017) The Elements of Physical Chemistry.Seventh Edition, Oxford Univ. Press.
6. Finar I. L. (2012) Organic Chemistry, Pearsons.
7. Morrison R.T. and Boyd R.N. (2009) Organic Chemistry, Seventh Edition, Pearsons.
8. Stryer L. (2015) Biochemistry, Eight Editions. W.H. Freeman.
9. Voet, D. and Voet, J.G. (2011) Biochemistry. Fourth Edition, John Wiley & Sons.
Reference :
1. Principles of physical chemistry- Puri,Sharma

2. Baxevanis A.D. And Ourllette BFF. (2001) Bioinformatics, Second edition, Wiley Interscience.
3. Mount D.W. (2004) Bioinformatics: Sequence and Genome Analysis, Second Edition. CSHL Press.
4. Lesk A.M. (2013) Introduction to Bioinformatics.Fourth Edition. Oxford University Press.
5. Grohima M.M. (2010)Protein Bioinformatics. Elsevier Publications.
6. Ghosh Z. and Mallick B. (2008)Bioinformatics: Principles and Applications. Oxford University Press.
Facilitating the achievement of Course Learning Outcomes
Module Course Learning Teaching and Assessment Tasks

Learning
Outcomes
Activity
I Students will be given basic Chalk and board Discussions, oral

concepts of chemistry of teachings, powerpoint questioning and
molecules where they will be presentations analytical questions
taught about some topics like will be given to
stereochemistry, chemistry and students. Internal
properties of water, acid base exams and seminars
concepts etc. will beconducted
II Students will learn about the Chalk and board Discussions, oral
proteins and their biochemical teachings, powerpoint questioning and
roles. presentations analytical questions
will be given to
students. Internal
exams and seminars
will beconducted
15
III Students will learn about the Chalk and board Discussions, oral
chemistry and biological functions of teachings, powerpoint questioning and
carbohydrates, nucleic acid and
lipids; glycoproteins & lipoproteins. presentations analytical questions
will be given to
students. Internal
exams and seminars
will beconducted
IV Students will learn and will be Chalk and board Discussions, oral
given basic Introduction to teachings, powerpoint questioning and
bioinformatics & computational presentations. analytical questions
biology; applications of will be given to
bioinformatics. students. Internal
exams and seminars
will beconducted
PAPER II:CELL BIOLOGY AND PHYSIOLOGY

Course Objective:
The course entails to educate the student about

 the basic biology and physiology of acell.
 the different types of cell structures and their diversefunctions
 to study the cell to cell interactions which help in different physiologicalfunctions
16
Detailed Syllabus:
Modules Topics / Course content Periods
Cellclassification
Cell classification: Cell variability (size, shape, complexity,

functions). Structuralorganisation of prokaryotic and
eukaryotic cells. The ultra-structure of nucleus, mitochondria,
endoplasmic reticulum, rough and smooth, Golgi apparatus,
lysosmesandperoxisomesandtheirfunctions.Thecytoskelton
I - microtubules and microfilaments. Types of tissues, 16
epithelium - types, epithelial apices - glycocalyx, microvilli.
The basement membrane - structural features and
characteristics. The extracellular matrix-collagen, elastin,
fibrillin, fibronectin, laminin and protroglycans. Plant and
animal cells: variation in structure and function.
Cell differentiation, and viral infections
Viruses: Structure and classification. Life cycle. Culture

techniques to study cell division – cell division by mitosis and
meiosis. Cell cycle. Cell differentiation - organogenesis,
II 14
morphological, functional and biochemical maturation of
tissues.
Biochemistry of blood
Blood: Composition and functions of plasma, erythrocytes

including Hb, leukocytes and thrombocytes plasma proteins
in health and diseases.
III Blood: Composition and functions of plasma, erythrocytes 16
including Hb, leukocytes and thrombocytes plasma proteinsin
health and diseases. Blood coagulation - mechanism and
regulation. Fibrinolysis, transfers of blood gases oxygen and
carbon dioxide. Role of 2,3 DPG, Bohr effect and chloride
shift.
17
Hydrogen ion homeostasis- Factors regulating blood pH -
buffers, respiratory and renal regulation. Acid-base balance -
metabolic and respiratory acidosis and alkalosis.
Biochemistry of organ systems
Digestive system: Composition, functions and regulation of

saliva, Gastric, pancreatic, intestinal and bile secretions -
digestion and absorption of carbohydrates, lipids, proteins,
nucleic acids, minerals and vitamins. Excretory system:
IV Structure of nephron, formation of urine, glomerular 18
filtration, tubular-absorption of glucose, water and
electrolytes - tubular secretion. Regulation of water and
electrolyte balance, role of kidneys and hormones in their
maintenance. The endocrine glands: secretion and function -
reproduction, pregnancy and lactation. Biochemistry of
vision.
Total 64
Text Books:
1. Molecular Biology of the Cell, 6th edition. Bruce Alberts, Dennis Bray, JulianLewis,
2. Martin Raff, Keith Roberts, and James D Watson. Publisher New York: GarlandScience.
3. The Cell: A Molecular Approach, 7th Edition, by Geoffrey M. Cooper and RobertE.
Hausman, published by ASMPress.
4. Lehninger Principles of Biochemistry, Nelson, D.L., Cox, M.M., WH Freeman and
Company, New York, USA. 7th edition, 2017
5. Molecular Cell Biology; Lodish et al., 8thEdn. W.H. Freeman and Co.(2012).
6. Biochemistry 8th Edn. Jeremy M. Berg, John L. Tymoczko, LubertStryer.
7. Victor Rodwell, David Bender, P. Anthony Weil, Peter Kennelly. Harpers Illustrated
Biochemistry 31th Edition,2018
Reference:
1. Lipid Biochemistry; 5th Edn. Michael I. Gurr, John L. Harwood and Keith N. Frayn,
Blackwell Science (2002).
2. Principles of Human Physiology; 6th Edn. Cindy L. Stanfield Pearson,(2011).
3. Biochemistry Ed. Donald Voet& Judith G. Voet, 4thEdn. John Wiley & Sons, Inc.
(2012).
4. Mammalian Biochemistry; White, Handler and Smith, McGraw-Hill,(1986)
18

Learning
Outcomes
Activity
I Students will learn about the Chalk and board Discussions,oral

cellular organisation, cell teachings, powerpoint questioning and
organelles, cytoskeleton, presentations analytical questions
tissue arrangement and the will be given to
differences in plant and students. Internal
animal cells exams and seminars
will beconducted
II Students will learn about the Chalk and board Discussions,oral

biochemistry of viral teachings, powerpoint questioning and
infection, cell culturing, presentations, video analytical questions
mitosis and meiosis, presentations on the will be given to
biochemistry of cell stepbystepprocessof students. Internal
differentiation, biochemistry mitosis and meiosis exams and seminars
of carcinogenesis, and the will be shown, will beconducted
factors affecting chronology of
carcinogenesis molecular events in
various processes will
be shown asflowchart
III Students will learn about the Chalk and board Discussions,oral
composition of blood, teachings, powerpoint questioning and
clotting mechanisms of presentations analytical questions
blood, role of various ions in will be given to
the blood and their effect on students. Internal
the blood, and the various exams and seminars
factors that affect the pH of will beconducted
blood and the subsequent
effect of change of blood pH
IV Students will learn about the Chalk and board Discussions, oral
digestive system, and the teachings,powerpoint questioning and
molecular components ofthe presentations, analytical questions
19
digestive system, digestion chronology of will be given to
and absorbtionof molecular events in students. Internal
carbohydrates, lipids, various processes will exams and seminars
proteins, vitamins, minerals, be shown asflowchart will be conducted
and the excretory system,the
working mechanisms of the
excretion, mechanism of
maintainence of water and
electrolyte balance, the
endocrine system and the
biochemistry ofvision
PAPER III:BIOENERGITICS AND METABOLISM

Course Objective:
 To extend a detail idea of bioenergetics andmetabolism.

 To educate students the concept of energy and metabolism.
 To enable detail understanding of the biological macromolecules likelipid,
carbohydrate proteins and nucleic acids.
Detailed Syllabus:
Bioenergetics: Energy transformation, Laws of

thermodynamics, Biological oxidations, oxygenases,
hydroxylases, dehydrogenases and energy transducing
membranes. Gibbs energy, free energy changes and redox
I potentials, phosphate potential, ion electrochemicalpotentials 18
,proton electrochemical potential, membrane potentials,
photons energy interconversions. Ion transport across energy
transducingmembranes.lnfluxandeffluxmechanisms.Proton
circuit and electrochemical gradient, the transport and
20
distribution of actions, anions and ionophores. Uniport,
antiport and symport mechanisms, shuttle systems. The
mitochondrial respiratory chain, order and organization of
carriers, proton gradient, iron sulphur proteins, cytochromes
and their characterization.
Intermediary metabolism: Approaches for studying

metabolism. Carbohydrates: Glycolysis, citric acid cycle its
function in energy generation and biosynthesis of energy rich
bonds,pentosephosphatepathwayanditsregulation.Alternate
II pathways of carbohydrate metabolism. Gluconeogensis, inter 14
conversions of sugars. Biosynthesis of glycogen, starch and
oligosaccharides. Regulation of blood glucose homeostasis.
Hormonal regulation of carbohydratemetabolism.
Lipids metabolism
Lipids Fatty acid biosynthesis: Acetyl CoA carboxylase, Fatty
acid synthase, desaturase and elongase. Fatty acid
oxidationand its regulation. Lipid Biosynthesis: Biosynthesis
of triacyglycerols, phosphoglycerides and
sphingolipids,Biosyntheticpathwaysforterpenes,steroidsand
III prostaglandins. Ketone bodies: Formation and utilisation. 16
Metabolism of Circulating lipids: chylomicrons, LDL, HDL
and VLDL. Free fatty acids. Lipid levels in pathological
conditions.
Metabolism of amino acidsandnucleotides Amino

Acids. Biosynthesis and degradation of amino acids. Urea
cycleanditsregulation,NucleicAcidsBiosynthesisorpurines and
pyrimidines Degradation of purines and pyrimidines.
IV 16
Regulation of purine and pyrimidine biosynthesis Structure
and regulation of ribonucleotide reductase. Biosynthesis of
ribonucleotides, deoxyribonucleotides and polynucleotides.
Inhibitors of nucleic acidbiosynthesis
Total 64
21
Text Books:
1. Lehninger Principles of Biochemistry, Nelson, D.L., Cox, M.M., WH Freeman and

Company, New York, USA. 7th edition, 2017
2. Harper‘s Illustrated Biochemistry; 31thEdn. Robert K. Murray, Daryl K. Granner,Victor
W. Rodwell, The McGraw-Hill (2018).
3. Biochemistry by Donald Voet and Judith Voet, John Wiley and Sons NY. 4th edtn,2012.
Reference :
1. Berg, J. M., Tymoczko, J. L. and Stryer.,Biochemistry,, W.H Freemanand Co., 9th edition,
2019.
2. Buchanan,B.,Gruissem,W.andJones,R.,BiochemistryandMolecularBiologyofPlants,
2ndEdn, American Society of Plant Biologists,USA.
3. Fundamentals of Biochemistry by J.L.Jain, Nitin Jain and Sunjay Jain, S.ChandGroup.

Learning
Outcomes
Activity
I Studentswilllearnaboutthelaw of Chalk and board Discussions,oral

thermodynamics, their teachings, powerpoint questioning and
application in bioenergetics, the presentations analytical questions
energetics of various involving calculations
biomolecules and their role in of bioenergetics will
carrying out biochemical be given to students.
reactions, the processes by Internal exams and
which electrochemical gradients seminars will be
are produced, the proteins conducted.
involved in generating
electrochemical gradients, the
mitochondrial electron transport
system and their role in energy
production and the protein and
inorganic groups that allow the
formation of mitochondrial
electrontransport
22
II Students will learn metabolism Chalk and board Discussions,oral
of carbohydrates, the associated teachings, powerpoint questioning and
bioenergetics and the extensive presentations analytical questions
regulatory mechanisms in place will be given to
to control carbohydrate students. Internal
formation and breakdown exams and seminars
will beconducted
III Students will learn about lipid Chalk and board Discussions,oral
metabolism, synthesis of fatty teachings, powerpoint questioning and
acids and different types of presentations analytical questions
complex lipids, lipid hormones, will be given to
roleoflipidmoleculeslikeLDL, students. Internal
HDL, and involvement of lipids exams and seminars
indiseases will beconducted
IV Students will learn about Chalk and board Discussions,oral

biosynthesis and breakdown of teachings, powerpoint questioning and
amino acids, nucleotides, their presentations analytical questions
regulation and inhibitors of will be given to
nucleotide biosynthesis students. Internal
exams and seminars
will beconducted
PAPER IV: PRACTICAL I

STUDENT’S SCHEME OF EVALUATION: PRACTICAL (P)
Course Objective:
 To educate the students about the practical aspects of Organic and BiophysicalChemistry,
 Cell biology, Physiology, Bioenergetics and intermediarymetabolism.
 Tointroducethestudentstobasicbiochemicaltestsfordetectionofbiomoleculesandtheir
estimations.
 To introduce the students to experiments to visualize and understand different cell
structures present in the body
23
Detailed Syllabus:

1. Preparation of buffer using Henderson-Hasselbach equation and
determination of its buffering capacity by acid andalkali.
I 2. Estimation of serum inorganic phosphate. 24
3. Formal titration of amino acids.
1. Estimation of DNA usingdiphenylamine.
II 2. Estimation of RNA usingorcinol. 24
3. Determination of free proline.
1. Estimation of Carbohydrate by Anthronemethods.
III 24
2. Estimation of protein by Lowrys method.
3. Microscopic examination ofurine.
4. Microscopic examination and chemical analyses ofblood.
1. Separation of amino acids mixtures by paper chromatography.
IV 2. Separation of lipids by TLC. 24
3. Molecular docking study of protein and drugs using MGL tools.
Total 96
Text books:
1. Practical Biochemisty – 3rdEdn. DavidPlummer.

2. Introductory Practical Biochemistry – S.K.Sawhney and Randhir Singh.
3. Practical Clinical Biochemistry, ed. Harold Varley, 4th edn. CBSPublishers.
4. Practical Clinical Biochemistry: Methods and Interpretation, 4th edn. RanjnaChawla,
Jaypee Brothers Medical Publishers .
5. Practical and Clinical Biochemistry for Medical Students, ed. T.N.Pattabhiraman,
GajannaPublishers.
24

Learning
Outcomes
Activity
I Students will understandthe Chalk and board Discussions, oral

process of teachings, questioning
makingbuffers,buffering video and
range, and the uses of buffer presentations of the analytical questions
in biochemical reactions, methology, will be given to
enzyme activity and blood calculationsguidance, students. Students
group variety testing conversion of moles, ‗hands on‘ learning
molar, mg, g, L etc, will be regularly
weighing, and monitored
handlingequipments
II Students will learn Chalk and board Discussions, oral
thecolourimetricestimation teachings, calculations questioning and
techniques for DNA, RNA, guidance, video analytical questions
techniques for separation of presentation of the will be given to
proteins methology, students. Students
conversion of moles, ‗hands on‘ learning
weighing, monitored
and
handlingequipments
III Students will learn to measure Chalk and board Discussions, oral
glucose in a givensample, teachings, calculations questioning and
carry out biochemical guidance, video analytical questions
analyses of urine and presentation of the will be given to
histologic analysis of methology, students. Students
biologicalfluids conversion of moles, ‗hands on‘ learning
weighing, monitored
and
handlingequipments
working mechanism and teachings, calculations questioning and
working protocol of RIA and guidance, video analytical questions
also carry out isolation of presentation of the will be given to
bacterial cultures from methology, students. Students
industrial effluents conversion of moles, ‗hands on‘ learning
weighing, monitored
and
25
handlingequipments
26
2ND SEMESTER
27
PAPER I: ENZYMOLOGY
Course Objective:
 Teach the students on the kinetic of enzyme catalysis; steady state enzyme and transient
kinetic.
 Provide students with opportunities to learn and understand the basic concept behind
enzyme and enzyme catalyzedreaction.
Detailed Syllabus:

Introduction to enzymes
Enzyme nomenclature and classification, Isozymes; Ribozymes;

Zymogens; Abzymes; Structure and General properties of
enzymes; Active site and Specificity of enzyme; Enzyme
substrate complex. Induced fit theory. Mechanism of enzyme
action, Factors affecting enzyme activity; Isozymes; Coenzymes,
I 16
Metalloenzymes; membrane bound enzymes; Multienzyme
complexes. Thermodynamics and Equilibrium; Enzyme activity;
Specific activity and Units; Classification and nomenclature of
enzymes.Enzyme assays: Types, Continuous and discontinuous
assays.
Enzyme kinetics
Kinetics of enzyme action – Concept of ES complex, active site,

specificity, derivation of Michaelis-Menten equation for uni-
substratereactions.Differentplotsforthedeterminationof Km &Vmax
II 18
and their physiological significances. Importance of Kcat/Km.
Kinetics of zero & first order reactions. Significance and
evaluation of energy of activation. Collision & transition state
theories. Michaelis–pH functions & their significance.
Classification of multi-substrate reactions with examples of each
class. Reversible and irreversible inhibition. Competitive, non-and
numerical basedon these. Suicide inhibitor.
28
Mechanism of Enzyme Action–Acid-base catalysis, covalent
catalysis, proximity, orientation effect. Strain & distortion theory.
Chemical modification of active site groups. Site directed
III mutagenesis of enzymes. Mechanism of action of chymotrypsin, 14
lysozyme, glyceraldehyde 3- phosphate dehydrogenase, aldolase,
carboxypeptidase, triosephosphate isomerise and alcohol
dehydrogenase
Enzyme Regulation –General mechanisms of enzyme regulation,
product inhibition. Reversible (glutaminesynthase &
phosphorylase) and irreversible (proteases) covalent
IV modifications of enzymes. Mono cyclic and multicyclic cascade 16
systems with specific examples. Feedback inhibition and feed
forward stimulation. Allosteric enzymes, qualitative description
of ―concerted‖ &―sequential‖models forallosteric enzymes. Half
site reactivity, Flip flop mechanism, positive and negative co-
operativity with special reference toaspartate transcarbamoylase&
phosphorfructokinase.
Text Books:
1. Fundamentals of Ezymology; 3rd Edn. Nicholas C. Price and LewisStevens,
OxfordUniversity Press(2012).
2. Lehninger Principles of Biochemistry; D.L.Nelson and M.M. Cox, 7thEdn.
MacMillanPublications(2017).
3. Principles of Biochemistry; Smith et al., Ed. McGrawHill,(1986).
4. Enzymes; Trevor Palmer, East – 2ndedn. West Press Pvt. Ltd., Delhi(2004).
5. Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis;
Robert A.Copeland , 2ndedn. Wiley-VCH Publishers(2000).
6. Enzyme Kinetics and Mechanism; Paul F. Cook, W. W. Cleland, GarlandScience
(2007).
7. Biochemical Calculations, Irwin H. Segel (1976) 2nd Ed. John Wiley andSons.
8. Methods in Enzymology; Colowick S.P. et al., Vol. 152, Academic Press,(1987).
9. Methods of Enzymatic Analysis; Bergmeyer Vol. 1-X, (1974).
10. Principles of Enzymology for Food Sciences; Whitaker, Marcel Dekker (1972)
AcademicPress.
11. Enzymes: Biochemistry, Biotechnology and Clinical Chemistry; TrevorPalmer
(Edn)Horwood Chemical Science Series.
12. Introduction to Enzyme and Co-enzyme Chemistry. Ed. T. Bugg, 3rdedn.(2012),
BlackwellScience.
Reference:
1. An Introduction to Enzyme and Coenzyme Chemistry; Timothy B. Bugg,(1997)

2. Enzyme Kinetics; Roberts, D.V. (1977), Cambridge UniversityPress.
3. The Enzymes; Boyer, 3rdedn. Academic Press,(1987).
4. Enzyme Kinetics; Irwin H. Segel (1976)Interscience-Wiley.
5. Enzyme Kinetics; the Steady state approach; Engel, P.C. (1981) 2nd Edn.
ChampmanandHall.
29
6. Nature of Enzymology; Foster, (1980), CroomHelm.

Learning
Outcomes
Activity
I Students will learn about Chalk and board Discussions, oral
different enzyme teachings, powerpoint questioning and
classifications, various aspects presentations analytical questions
of action mechanism of enzyme, will be given to
role of metals in enzyme action, students. Internal
organisationof enzymes in cells exams and seminars
and enzyme assays will beconducted
II Students will learn about Chalk and board Discussions, oral
enzyme kinetics, teachings, powerpoint questioning and
michealismenten equation, presentations analytical questions
will be given to
derivationsof
students. Internal
michealismentenequation, role exams and seminars
of pH, temperature in enzyme will beconducted
activity and different inhibitors
and their working mechanism
III Students will learn about Chalk and board Discussions, oral
mechanisms of enzyme teachings, powerpoint questioning and
catalysis, different groups presentations analytical questions
will be given to
associated with enzyme
students. Internal
catalysis and the reaction exams and seminars
mechanisms of a few enzymes will beconducted
IV Students will learn about Chalk and board Discussions, oral

reversible, irreversibleand teachings, powerpoint questioning and
allosteric presentations analytical questions
will be given to
regulatio
students. Internal
n mechanisms with elaborate exams and seminars
examples will beconducted
30
PAPER I: MOLECULAR BIOLOGY
Course Objective:
 to educate students as wellas todemonstrate knowledge and understanding of the

molecular machinery of livingcells
 demonstrate knowledge and understanding of the principles that govern the structures of
macromolecules and their participation in molecularrecognition.
Detailed Syllabus:

Central dogma; Replication of genetic material in viruses, pro- and
eukaryotes and organelles; Transcription in pro- and eukaryotes.Regulation
I of replication and transcription. 16
Post-transcriptional processing & maturation; RNA: splicing and alternative

splicing, editing; Genetic code; Translation in pro- and eukaryotes; Post-
translational processing; Translational regulation of gene expression;
II Protein trafficking; Concept and applications: genome, transcriptome, 16
proteome metabolome and CRISPR/Cas technology.
Gene cloning: General strategy, DNA manipulating enzymes – restriction

endonucleases; Restriction modification; Types of vectors: Plasmid
III (expression, reporter, helper, cosmid etc.), Lambda, bacterial & eukaryotic;
Selection of recombinant clones; Genomic & cDNA banks and libraries.
16
Restriction analysis (shotgun cloning, RFLP, chromosome walking); Genetic
finger printing; PCR – reverse and real time; Applied biotechnology:
Molecular and antisense probes, micro & siRNA, gene silencing and its
IV uses; Transgenics, Human genome; SNP; Functional genomics: concept and
applications. 16
TOTAL 64
31
Text Books
1. Lewin B. Genes IX(2011)& Genes XII (2017), Jones & Bartlett Publ.
2. Alberts B., Johnson A., Lewis J., Raft M., Robert K. and Walter P. (2015) Molecular Biology of the Cell, Sixth
Edition, Garland Sci. Publ.
3. Glick B.R. and Pasternack J.J. (2009) Molecular Biotechnology, ASM Press.
4. Maniatis T., Fritsch and Sabrook J. (2014) Molecular Cloning, CHS.
5. Primrose S.B., Twiman R.M. and Old R. W. (2014) Principles of Gene Manipulation, Blackwell Scientific Publ.
6. Sam brook J. and Russell D. W. (2014) Molecular cloning, Vol.I& 2, CSHL Press.
7. Walker T.M. and Rapley R. (2015) Molecular Biology and Biotechnology, Royal Soc. Chem.
8. Valentine RC (2015) Molecular Biology and Evolution, CRC Press.
9. Lodish (2013) Molecular Cell Biology, WH Freeman &CO.
10. Trop P.B.E. (2011) Molecular Biology Genes to Proteins, Jones & Bartlett Publisher.
11. Watson, J.D. et al. (2014) Molecular Biology of the Gene, 7th Ed., Benjamin Cummings Publ.
12. Hardin et al. (2015) Becker's World of the Cell, Ninth edition, Pearson, U. K.
13. Pollard et al. (2017) Cell Biology, Third edition, Elsevier IE.
14. Cooper G. (2018) The Cell: A Molecular Approach, Eighth edition, Oxford University Press, NC USA.
References
1. Lodish (2013) Molecular Cell Biology, WH Freeman &CO.
2. Trop P.B.E. (2011) Molecular Biology Genes to Proteins, Jones & Bartlett Publisher.
3. Watson, J.D. et al. (2014) Molecular Biology of the Gene, 7th Ed., Benjamin Cummings Publ.
4. Hardin et al. (2015) Becker's World of the Cell, Ninth edition, Pearson, U. K.
5. Pollard et al. (2017) Cell Biology, Third edition, Elsevier IE.

Learning
Outcomes
Activity
I Students will learn aboutthe Chalk and board Discussions,oral

central dogma of life. teachings, power questioning and
point presentations analytical questions
will be given to
students. Internal
exams and seminars
will beconducted.
II Students will learn about Chalk and board Discussions,oral
the post-transcriptional teachings, power questioning and
processing & maturation, point presentations analytical questions
will be given to
translation, genetic code as
students. Internal
well as on protein exams and seminars
trafficking. will beconducted
III Students will learn the Chalk and board Discussions,oral
about the concept of gene teachings, power questioning and
cloning. point presentations analytical questions
will be given to
students. Internal
32
exams and seminars
will beconducted
IV Students will learn Chalk and board Discussions,oral

aboutvarious recombinant teachings, power questioning and
DNA based technologies point presentations analytical questions
will be given to
students. Internal
exams and seminars
will beconducted
PAPER III:IMMUNOLOGY
Course Objective:
 To introduce the students to the subjectImmunology

 To introduce students to immune system, cells involved in immune responses, lymphocytes, stem
cellsetc.
 To study how the immune system of an organism works and learn themolecular
mechanism involved theirfunctioning
Detailed Syllabus:
Introduction to Immune System: Memory, specificity, diversity,

innate and acquired immunity, self vs non-self discrimination. Structure
and functions of primary and secondary lymphoid organs. Cells
Involved in
I ImmuneResponsesPhagocyticcellsandtheirkillingmechanisms.Tand B 16
lymphocytes. Differentiation of stem cells and idiotypic variations.
Nature of Antigen and Antibody. Antigen vs lmmunogen,
HaptensStructure and functions of immunoglobulins. lsotypic, allotypic
and idiotypicvariations.
33
Generation of Diversity in Immune System: Clonal selection theory -
concept of antigen specific receptor. Organization and expression of
immunoglobulin genes: generation of antibody diversity. T cellreceptor
diversity. Humoral and Cell Mediated immune Responses. Kinetics of
primary and secondary immune responses. Complement activation and
II its biological consequences. Antigen processing and presentation. 16
Cytokines and co stimulatory molecules: Role in immune responses. T
and B cell interactions. Major Histocompatibility Complex (MHC)
Genes and Products. Polymorphism of MHC genes. Role of MHC
antigens in immune responses. MHC antigens intransplantation.
Applications of immune reactions
Development, Regulation and Evolution of the Immune System

Measurement of Antigen - Antibody Interaction. Production of
III polyclonal and monoclonal antibodies: Principles, techniques and 16
applications. Agglutination and precipitation techniques. Radio
immunoassay.Immunofluorescence assays: Fluorescence activated
cell sorter (FACS) technique. Measurement of TCell
Activation.Fractionofleukocytesondensitygradient.Delayedtype
hypersensitivity technique. Leukocyte migration inhibition technique.
Cytotoxicity assay. Cytokines assay: ELISA and ELISPOT
IV Immune associated diseases 16
Tolerance vs Activation of immune System. Immune –tolerance.
Immunosuppression. Hypersensitivity (Types I, II, III and IV).Immune
Responses in Diseases. Immune responses to infectious diseases: viral,
bacterial and protozoal, Cancer and immune system. Immunodeficiency
disorders, Autoimmunity. Immunization, Active immunization
(immunoprophyiaxis). Passive immunization (Immunotherapy). Role of
vaccines in the prevention of diseases.
64
TOTAL
Text Books:
1. Kuby Immunology; Oven, Punt, Stranford, 8thEdn. W. H. Freeman(2018).

2. Roitt‘s Essential Immunology; Ivan, M. Rohitt&Petrer J Delves 13th (2011) Blackwell
Science.
3. Immunology: Roitt et al., Mosby(2001).
4. Immune System; M. C. Connel et al., Eds. (1981) BlackwellScience.
5. Immunology at a Glance: J.H.L. Playfare [ed.] Blackwell Science,(1987).
6. Immunology; Jan Klein [Ed.], Blackwell Science(1990).
34
7. Introduction to Immunology; Kim Bell [Ed.,] 3rdEdn. McMillan(1990).
.
Reference:
1. Antibodies– A Laboratory Manual; E. D. Harlow, David Lane, 2nd Edn. CSHLPress

(2014).
2. Basic and Clinical Immunology; Stites et al., [6thedn] (2011)Lange.
3. Veterinary Immunology: Ian R. Tizard, 10thEdn. I.R. Thomsonpress.
4. The Immune System. By Peter Parham 4thEdn. Publisher Garlandpublishing

Learning
Outcomes
Activity
I Students will learn about the various Chalk and board Discussions,oral
levels of immune systems, the cells teachings, powerpoint questioning and
associated with immune system, presentations analytical questions
about different types of antigens and will be given to
antibodies students. Internal
exams and seminars
will beconducted
II Students will learn about the Chalk and board Discussions,oral
generation of diversity in antibodies teachings, powerpoint questioning and
and immune cell receptors, the presentations analytical questions
genetic causes of such diversity, the will be given to
humoral and cell mediated immune students. Internal
system, antigen presentation, antigen exams and seminars
processing, compliment system, will beconducted
cytokines and theissues
withtransplantation
III Students will learn about the many Chalk and board Discussions,oral
immune based assays that are used in teachings, powerpoint questioning and
immunology and molecular biology presentations analytical questions
research like measurement of will be given to
antigen-antibody complexes, students. Internal
and methods like ELISA, RIA,FACS exams and seminars
will beconducted
IV Students will learn about immune Chalk and board Discussions,oral
suppression, tolerance, teachings, powerpoint questioning and
hypersensitivity, immune responses to presentations analytical questions
diseases, like cancer, autiimmune will be given to
diseases, vaccination and role of students. Internal
vaccine in disease prevention exams and seminars
will beconducted
35
PAPER IV:PRACTICAL II
STUDENT’S SCHEME OF EVALUATION: PRACTICAL (P)
Course Objective:
 To learn the techniques of Western Blot, Electrophoresis, kinetic studiesetc.

 To introduce the students to electrophoretic techniques used for separation of DNAand
proteins
 To introduce the students to basic biochemical tests for detection of biomoleculesand
theirestimations.
Detailed Syllabus:
1. Isolation of protein and its estimation by Bradford

method.
2. Assay of Vmax and Km of enzymes like salivary
I 24
amylase and alkaline phosphatases.
3. Determination of effect of pH on salivary amylase.
1. Blood grouping.
2. Enumeration ofWBC‘s
II 3. Radial immunodiffusion assay. 24
4. Double diffusion method.
1. Isolation of DNA andAgarose gel electrophoresis.

III 2. Isolation of RNA andAgarose gel electrophoresis. 24
3. Isolation of proteins and its separation using SDS
PAGE.
1. Demonstration of ELISA.
2. Demonstration of PCR
IV 3. Designing of primers. 24
4. Demonstration of western blotting.
Total 96
Text Book:
36
1. Biochemistry, ed. Plummer Tata 3rdEdn.-McGraw Hill,.
2. Practical Clinical Biochemistry, ed. Harold Varley, 4th edn. CBS Publishers.
3. Hawk‘s Physiological Chemistry, ed. Oser, 14th Edn.,Tata-McGrawHill.
Reference:
1. Practical Clinical Biochemistry: Methods and Interpretation, 4thedn. Ranjna Chawla,
Jaypee Brothers Medical Publishers .
2. Practical and Clinical Biochemistry for Medical Students, ed. T.N.Pattabhiraman,
GajannaPublishers.

Learning
Outcomes
Activity
I Students will learn about the Chalk and board Discussions, oral
colourimetric estimation of teachings, questioning and
biomolecules like glucose, calculations guidance, analytical questions
proteins and vitamin C video presentation of will be given to
the methology, students. Students
handlingequipments
II Students will learn about the Chalk and board Discussions, oral
estimation of haemoglobin teachings, questioning and
and measurement of blood calculations guidance, analytical questions
cells like WBC, RBC video presentation of will be given to
handlingequipments
III Students will leran about the Chalk and board Discussions, oral
sepertion of biomolecules teachings, questioning and
using different techniques calculations guidance, analytical questions
video presentation of will be given to
conversion ofmoles, ‗hands on‘ learning
37
handlingequipments
effect of substrate, pH, teachings, questioning and
temperature on enzyme calculations guidance, analytical questions
activity and also shall get a video presentation of will be given to
demonstration of HPLC and the methology, students. Students
GLC conversion of moles, ‗hands on‘ learning
handlingequipments
38
3RD SEMESTER
39
PAPER I: ADVANCED ENZYMOLOGY
Course Objective:
 The objective of the course is to introduce studentsto
 to provide a deeper insight into the fundamentals of enzymestructure
 function and kinetics of soluble and immobilizedenzymes.
 deals with current applications and future potential ofenzymes.
Detailed Syllabus:
Module Topics (if applicable) & Course Contents Periods
s
Review of unisubstrate enzyme kinetics and factors affecting
the rates of enzyme catalyzed reactions. Michaelis pH functions
and their significance.
I
Classification of multisubstrate reactions with examples of each
12
class.Kinetics of multisubstrate reactions.Derivation of the rate
of expression for Ping Pong and ordered Bi Bi reaction
mechanism .Concept of Convergent and Divergent evolution of
enzymes.Flexibility and conformational mobility of enzymes.
Enzymes Turnover and methods employed to measure
Turnover of enzymes. Significance of enzymes Turnover.
Protein - Ligand binding, including measurement, analysis of
II
binding isotherms. Co- operativity phenomenon. Hill and
12
Scatchard Plots. Allosteric enzymes, Sigmoidal kinetics, and
their physiological significance. Symmetric and sequential
modes for action of allosteric enzymes and their significance.
Immobilized enzymes and their industrial applications.
Multienzyme system: Occurrence, isolation, and their properties.
Polygenic nature of multienzyme systems. Mechanism of action
and regulation of pyruvate dehydrogenase and fatty acid 12
III
synthetase complexes. Immobilized Multienzyme Systems and
their applications.
Co-enzymes and cofactors: Water soluble vitamins as
coenzymes. Metallo enzymes.
40
Detailed Mechanisms of Catalysis of serine proteases.
Ribonuclease, and Triose phosphate isomerases. Enzyme
regulation: General mechanisms of enzyme regulation: Feed 12
Back Inhibition and Feed forward stimulation; Enzyme
IV
repression, induction and degradation, control of enzymatic
activity by products and substrates; Reversible and irreversible
covalent modifications of enzymes.
Text books:
1. Enzymes; Trevor Palmer, East –2nd edn. West Press Pvt. Ltd., Delhi(2004).
2. Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis;Robert
A.Copeland ,2nd edn. Wiley-VCH Publishers(2000).Biochemical Calculations, Irwin H. Segel
(1976) 2nd Ed. John Wiley andSons.
3. Methods in Enzymology; Colowick S.P. et al., Vol. 152, Academic Press,(1987).
4. Methods of Enzymatic Analysis; Berg Meyer Vol. 1-X, (1974).
5. Principles of Enzymology for Food Sciences; Whitaker, Marcel Dekker (1972)Academic
Press.
6. Enzymes: Biochemistry, Biotechnology and Clinical Chemistry; TrevorPalmer
(Edn)Horwood Chemical ScienceSeries.
Reference
1. Fundamentals of Ezymology; 3rd Edn. Nicholas C. Price and LewisStevens,
OxfordUniversity Press(2012).
2. Lehninger Principles of Biochemistry, Nelson, D.L., Cox, M.M., WH Freeman
andCompany, New York, USA. 7th edition, 2017
3. Principles of Biochemistry; Smith et al., Ed. McGrawHill,(1986).
4. Introduction to Enzyme and Co-enzyme Chemistry. Ed. T. Bugg,3rd edn. (2012), Blackwell
Science.
41
Unit No. Course Learning Outcomes Teaching and Assessment Tasks

Learning Activity
I Students will learn the unisubstrate Power point Students will be

and multisubstrate enzyme kinetics; presentations; Teaching asked
they will also understand the using chalk and board; questions;Quiz,
Oral discussion sessions
concept of Convergent and internal assessment
in the
Divergent evolution of enzymes. Class tests will be
Flexibility and conformational
mobility of enzymes. conducted.
II Students will learn about enzymes Power point Students will be

Turnover and methods employed to presentations; Teaching asked
measure Turnover of enzymes; they using chalk and board; questions;Quiz,
Oral discussion sessions
will also learn Allosteric enzymes internal assessment
in the
and symmetric and sequential modes Class tests will be
for action of allosteric enzymes
conducted.
III Students will understand Students will be asked Students will be

multienzyme system and Polygenic to orally revise the asked questions;
nature of multienzyme systems; they previous class before Mid-term internal
every new class helping
will also learn different coenzymes assessment
them in better
and cofactors understanding and their tests will be
doubts cleared, ifany. conducted.
Teaching will be
conducted both through
white board mode and
power point
presentation
mode.
IV Students will learn detailed Teaching will be Students will be
Mechanisms of Catalysis of serine conducted both through asked questions;
proteases. Ribonuclease, and Triose black board mode and Internal assessment
power point
phosphate isomerases. tests (mid-term and
presentation
Enzyme regulation; they will also mode. Previous day‘s end-term) will
learn various mechanisms of class be conducted.
enzyme regulation revised before start of
teaching
42
PAPER II:CLINICAL BIOCHEMISTRY
Course Objective:
The objectives of the course is to introduce students to
 The objectives of this course are to demonstrate, through lectures and other labbased
methods, how basic biochemistry and analytical chemistry can be applied to medical
diagnosis, treatment and management.
 It will use examples within human system to demonstrate clinical disorders, the
biochemical consequences of disease process and the response totherapy.
Detailed Syllabus:
Module s Topics (if applicable) & Course Contents Periods
I Specimen collection and analysis: 12

Concepts of accuracy, Precision, Reliability, Reproducibility, Normal
values, Specimen collection and processing, Blood Collection-
Anticoagulants, Venipuncture, Urine collection, CSF, Aminotic fluid, pH of
blood, acid base equilibrium, sodium, potassium, chloride, bicarbonate.
II Inborn errors of metabolism: 12
Carbohydrate metabolism: galactosemia, Normal levels, renal threshold,
Factors influencing blood glucose, Glycogen storage
disorders,Pentosuria,Aminoacidmetabolism-alkaptonuria,maple syrup urine
disease, phenylketonuria, homocystinuria,proteinuria, albinism,
multiplemyeloma.
Lipid metabolism: Hyperlipidemia, Hyperlipoproteinemia, Fatty
liver.
Nucleotide metabolism: Lesch-Nyhan Syndrome, Biochemistry of anemia,
thalassemia, porphyria
III Disease caused by metabolic disorders of endocrine glands: Diabetes, 12
obesity, atherosclerosis, Conn‘s syndrome, Addison‘s disease, Cushing‘s
syndrome, hypo- and hyperthyroidism, gonadal dysfunction, dwarfism and
gigantism,
Diseases caused by chromosomal abnormalities-
Down, Turner and Klinefelter syndromes.
IV Cancer Biochemistry: - carcinogenesis, 12
characteristicsofcancercell,agentspromotingcarcinogenesis Cellular
differentiation, carcinogen, diagnosis of cancer, treatment ofcancer.
43
Total 48
44

Learning Activity
I Students will learn the method Teaching will be Oral questions will be asked;
specimen collection and analysis; conducted both through students will be asked to discuss
they will learn different aspects of the topic. Quiz, internal
power point
blood and urine collection. assessment
presentation
mode. tests will be conducted.
II Students will gain knowledge on Students will be asked Students will be
inborn errors due to defects in to orally revise the evaluatedthrough class
carbohydrate, protein and lipid previous class before discussion,assignments and
every new class tests. Students willbeassigned
metabolism
helping them in better various topics and willbe asked
understanding and to deliver a powerpoint
their doubts presentation on the assigned
cleared, if any. topics.
Teaching will be
conducted both
through white board
mode and power point
presentation mode.
III Students will learn about diseases Students will be asked Students will be evaluated
caused by metabolic disorders of to orally
through class
endocrine glands such as diabetes, revise the previous discussion,assignments and
obesity, atherosclerosis, Conn‘s class before starting tests. Students willbe
syndrome etc. the new topic.
Teaching will be assigned various topics and will
conducted both be asked to deliver a powerpoint
through white board presentation on the assigned
mode and power point topics.
presentation mode.
IV Students will learn about various Students will be Students will be evaluated
biochemical phenomena occur in asked to orally
through class
cancer, diagnosis of cancer, and its
revise the previous discussion,
treatment
assignments and tests. Students
class before every willbe
new class helping assigned various topics
them in better and will
understanding and be asked to deliver a
45
powerpoint
their doubts
cleared, if any. presentation on the
Teaching will be assigned topics.
conducted both
through white
board mode and
power point mode.
PAPER III: Practical III

STUDENT’S SCHEME OF EVALUATION: Practical Papers (P)
Course objective:
The course aims to develop skills of performing basic biochemical tests important in clinical
investigations, to develop familiarity with biochemical laboratory techniques, and to introduce
students to various practical aspects of enzymology and their correlation in disease conditions.
Course contents:
1. Determination of activation energy of enzyme.

2. Determination of influence of temperature on enzyme
I activity. 24
3. Immobilisation of urease in calcium alginate beads.
1. Determination of type of inhibition (reversible or

irreversible) of amylase.
II 2. Modelling of an enzyme active site using in silico 24
method.
46
1. Estimation of glucose by Folin Wumethod.
2. Estimation of cholesterol by Zack‘smethod.
III 3. Estimation of haemoglobin by Wong‘smethod 24
4. Estimation of urea in blood by Diacetylmonoximemethod.
5. Estimation of serum calcium by Clark and Collipsmethod.
1. Determination of A/G ratio by Biuretmethod.

2. Analysis of SGOT-SGPT (AST, ALT) / creatine kinase/
acid or alkalinephosphatase.
3. Qualitative analysis of Urine sample for normaland
abnormalconstituents.
4. Estimation of uric acid in serum and urine by Caraway‘s
IV 24
method
5. Determination of urine Chloride by Volhard-Arnold
method.
6. Estimation of urineBilirubin.
Total 96
Recommended readings:
1. Biochemistry, ed. Plummer Tata 3rd Edn.-McGrawHill,.

2. Practical Clinical Biochemistry, ed. Harold Varley, 4th edn. CBS Publishers.
4. Practical Clinical Biochemistry: Methods and Interpretation, 4th edn. Ranjna Chawla,Jaypee
Brothers Medical Publishers.
5. Practical and Clinical Biochemistry for Medical Students, ed. T.N. Pattabhiraman,Gajanna
Publishers.

Learning
47
Outcomes Activity
I Students will learn the Chalk and board Discussions, oral

determination of activity of teachings, video questioning and
selected enzymes, Km and presentations of the analytical questions
Vmax value, optimim pH, methology, will be given to
optimum temperature and calculations guidance, students. Students
activation energy of enzymes conversion of moles, ‗hands on‘ learning
handlingequipments
II Students will learn the Chalk and board Discussions, oral

determination of activity of teachings, questioning and
selected enzymes, Km, Vmax calculations guidance, analytical questions
and reversible or irreversible video presentation of will be given to
inhibition of enzymes the methology, students. Students
handlingequipments
III Students will the Chalk and board Discussions, oral

determination of various teachings, questioning and
biochemical markers in blood calculations guidance, analytical questions
such as glucose,haemoglobin video presentation of will be given to
concentration, cholesterol, the methology, students. Students
urea, andcalcium conversion of moles, ‗hands on‘ learning
handlingequipments
IV Students will learn the Chalk and board Discussions, oral

determination of various teachings, questioning and
biochemical marker present calculations guidance, analytical questions
in urine such as video presentation of will be given to
SGOT,SGPT, uric acid, the methology, students. Students
chloride, andbilirubin conversion of moles, ‗hands on‘ learning
handlingequipments
48
TH
4 SEMESTER
49
PAPER I: MICROBIOLOGY
Course Objective:
The objective of the course is to introduce students to
 to introduce basic principles and application relevance of clinicaldisease
 the content of course includes many etiological agents responsible for globalinfectious
diseases. It covers all biology of bacteria, viruses and other pathogens related with
infectious diseases inhumans.
 The course will provide the conceptual basis for understanding pathogenic
microorganisms and particularly address the fundamental mechanisms oftheir
pathogenicity.
 It will also provide opportunities for a student to develop diagnostic skills in
microbiology, including the practical application and interpretation of laboratory tests for
the diagnosis of infectiousdiseases.
Detailed Syllabus:
MODULE TOPICS/ CONTENTS PERIODS
Introduction to Microbiology and Diversity:Contributions of Anton von
Leeuwenhoek, Louis Pasteur, Robert Koch, Joseph Lister, Alexander
Fleming, Development of the field of soil microbiology: Contributions of
Martinus W. Beijerinck, Sergei N. Winogradsky, Selman A. Waksman, Paul
Ehrlich, Elie Metchnikoff and Edward Jenner. Systems of classification
(Binomial Nomenclature, Whittaker‘s five kingdom and Carl Woese‘s three
I 14
kingdom classification systems and their utility). Difference between
prokaryotic and eukaryotic microorganisms
General characteristics of different groups: Acellular microorganisms
(Viruses, Bacteriophages, Viroids, Prions) and Cellular microorganisms
(Bacteria, Algae, Fungi and Protozoa) with emphasis on distribution and
occurrence, morphology, mode of reproduction and economic importance.
50
Microbial genetics, Physiology and Metabolism:
Genomeorganization. coli, Saccharomyces.
Organelle genome: Chloroplast and Mitochondria. Types of plasmids
– F plasmid, R Plasmids, colicinogenic plasmids, Ti plasmids, linear
plasmids, yeast-2 μ plasmid.
Transformation, Conjugation (mechanism, Hfr and F‘ strains,
II 14
Interrupted mating technique and time of entry mapping).
Transduction (Generalized transduction, specialized transduction).
Definitions of growth, Batch culture, Continuous culture, generation
time and specific growth rate
Nutritional categories and requirements of microorganisms.
Concept of aerobic respiration, anaerobic respiration and fermentation.
Industrial and Medical Microbiology:
Microorganisms in industries – citric acid and lactic acid
production, production of beer and wine.
III Food borne infection and food borne intoxication. 10

Epidemiology of bacterial, fungal and viral diseases,
production of antibiotics from microorganisms and
chemotherapy.
Tools and Techniques in Microbiology:

Culture media: components of media, natural and synthetic media,
chemically defined media, complex media, selective, differential, indicator,
enriched and enrichment media.
Pure culture isolation: Streaking, serial dilution and plating methods;
cultivation, maintenance and preservation/stocking of pure cultures; Stain
IV and staning techniques. 10

Microscopy and its different types.
Asexual methods of reproduction, logarithmic representation of bacterial
populations, phases of growth, calculation of generation time and specific
growth rate.
Physical and Chemical methods of microbial control: Types and mode of
action.
TOTAL 48
Text books:
1. Lehninger Principles of Biochemistry, Nelson, D.L., Cox, M.M., WH Freeman andCompany,

51
New York, USA. 7th edition, 2017
2. Prescott, Harley and Klein‘s Microbiology, J.M. Willey, L.M. Sherwood and C.J.Woolverdon
(2017), 10th ed.,McGraw Hill Publishers.
3. Frazier and Westhoff. Food Microbiology 5th Edition, McGrawHill
4. Brock et al. Jawetz, Milnick and Adelberg‘s Medical Microbiology. 27th Edn (2016) Lange
Me
5. Biology of Microorganisms, 15th Edn. Brock Prentice Hall(2017).
6. Industrial Microbiology; Miller and Litsky (Eds.) (1976) McGraw HillPublishers.
7. Microbiology; Lansing M. Prescot, Hartley and Klein, 5th Edn. McGraw Hill(2002).
8. Microbiology; Essentials and Applications, Larry Mckane and J. Kandel (19)2nd
Edn.McGraw Hillpublishers.
Unit No. Course Learning Outcomes Teaching and Learning Assessment Tasks
Activity
I Students will learn the Students will be asked to Oral questions will
difference between orally revise theprevious be asked in
prokaryotes and class beforeeverynew class the class.
eukaryotes; they will also helpingthem in Problems will
learn the bacterial betterunderstanding and be assigned to
morphology and growth their doubtscleared, if test student‘s
pattern any.Videos will be analytical ability.
shown in the class Class tests will
for a better
understanding of be conducted for
the concepts. internal
Teaching will be assessment.
conducted both
through white board
mode and power
point presentations
mode
II Students will understand Students will be Oral questions will
about microbial diversity be asked in
learning fungi, algae, asked to orally
bacteria and viruses revise the previous the class. Problems
class before every will be
new class helping
them in better assigned to test
understanding and student‘s
their doubts cleared, if any. analytical ability.
Videos will be shown in the Class tests will
class for a better
be conducted for
understanding of the concepts.
52
Teaching will be conducted internal
both through white board assessment.
mode and power point
presentations
mode
III Students will learn about Students will be asked to Oral questions will
pathogenicity of orally revise theprevious class be asked in
microorganisms, their beforeevery
mode of action, and new classhelping the class. Problems
epidemiology, they will them in better will be
also understand the understanding and
assigned to test
industrial uses of
their doubts student‘s
microorgnaisms
cleared, if any. analytical ability.
Teaching will be Class tests will
conducted both
through white board mode and be conducted for
power internal
point presentations mode assessment.
IV Students will learn about Students will be Oral questions will
different microscopy, their asked to orally revise the be asked in the class.
working principle, previous Problems will be
applications, differences class beforeevery assigned to test
student‘s
new classhelping
them in better analytical ability.
Class tests will
understanding and
their doubts be conducted for
cleared, if any. internal
Videos will be assessment.
shown in the class
for a better
understanding of
the concepts.
Teaching will be
conducted both
through white board
mode and power
point presentations
mode
53
PAPER II:MEMBRANE BIOLOGY
Course Objective:
The objective of the course is to introduce students to
 Students will understand the structures and purposes of basic components ofprokaryotic
and eukaryotic cells, especially macromolecules, membranes, andorganelles
 Students will apply their knowledge of cell biology to selected examples of changesor
losses in cellfunction.
Detailed Syllabus:
Module Topics (if applicable) & Course Contents Perio
s ds
I Introduction to bio membranes: Composition of bio membranes - prokaryotic, 12
eukaryotic, neuronal, and subcellular membranes.
Study of membrane proteins. Fluid mosaic model with experimental proof.
Monolayer, planer bilayer and liposomes as model membrane systems.
II Membrane structures: Polymorphic structures of amphiphilic molecules in 10

aqueous solutions - micelles and bilayers. CMC, critical packing parameter.
Membraneasymmetry. Macro and micro domains in membranes. Membrane
skeleton, lipid rafts, caveolae and tight junctions. RBC membrane architecture.
III Membrane dynamics: Lateral, transverse, and rotational motion of lipids and 12
proteins. Techniques used to study membrane dynamics
- FRAP, TNBS labeling etc. Transition studies of lipid bilayer,
transition temperature. Membrane fluidity, factors affecting membrane
fluidity.
IV Membrane transport: Thermodynamics of transport. Simple diffusion and 14
facilitated diffusion. Passive transport - glucose transporter, anion transporter
and porins. Primary active transporters - P type ATPases, V type ATPases, F
type ATPases. Secondary active transporters - lactose permease, Na+ -glucose
symporter. ABC family of transporters - MDR, CFTR.Group translocation. Ion
channels - voltage-gated ion channels (Na+ /K+
voltage-gated channel), ligand-gated ion channels (acetyl choline receptor),
aquaporins, bacteriorhodopsin. Ionophores -
valinomycin, gramicidin.
TOTAL 48
67
Text books:
1. Lehninger Principles of Biochemistry, Nelson, D.L., Cox, M.M., WH Freeman andCompany,

New York, USA. 7th edition, 2017
2. Victor Rodwell, David Bender, P. Anthony Weil, Peter Kennelly. Harpers Illustrated
Biochemistry 31th Edition,2018
3. Text book of medical biochemistry by M N Chatterjea, Rana Sindhe. 8thEdn
4. Text book of medical biochemistry by S.Ramakrishnan, K G Prasannan. 3rdEdn.
5. J.M. Berg, J.L. Tymoczko, L. Stryer. . Biochemistry,9th Edn.(2019) WH Freemanand
Company, New York and England.
6. R. Verna.. Membrane Technology, Raven Press, New York., USA.
7. H. Lodish, A. Berk, S.L. Zipursky, P. Matsudaira, D. Baltimore, J. Darnell.. MolecularCell
Biology,8th Edn. WH Freeman and Company, NY andEngland
8. H.R. Petty.. Molecular Biology of Membranes Structure and Function, Plenum Press,New
York, USA andLondon.
9. D.F.H. Wallach.. Membrane Molecular Biology of Neoplastic Cells, (1975) ElsevierScientific
Publishing Company, Amsterdam, Oxford and New York.,USA.
Unit No. Course Learning Teaching and Learning Assessment Tasks

Outcomes Activity
I Students will learn about Students will be asked to orally Oral questions will be
various biomembranes, revise the previous class before asked in
structure, fluid mosaic every new class helping them in the class. Problems will
better beassigned to test
model, and liposome
understanding and their doubts student‘sanalytical
cleared, if any.Videos will be ability. Class tests will
shown in the class for a better be conducted for internal
understanding ofthe concepts. assessment.
Teaching will beconducted both
through white boardmode and
power
point presentationsmode
68
II Students will learn about Students will be asked to orally Oral questions will be
micelles, membrane revise the previous class before asked in
asymmetry, macro and every new class helping them in the class. Problems will
better be assigned to test
micro domains in
understanding and their doubts student‘s analytical
membranes, lipid rafts, cleared, if any.Videos will be ability. Class tests will
caveolae, tight junctions shown in the class for a better be conducted for internal
and RBC membrane understanding of the concepts. assessment.
architecture. Teaching will be conducted both
through white board mode and
power
point presentations mode
III Students will understand Students will be asked to orally Oral questions will be
membrane dynamics revise the previous class before asked in
through learning lateral, every new class helping them in the class. Problems will
transverse and rotational
motion of lipids and cleared, if any.Videos will be ability. Class tests will
proteins, and different shown in the class for a better be conducted for internal
techniques to study understanding of the concepts. assessment.
membrane dynamics Teaching will be conducted both
power
IV Students will understand Students will be asked to orally Oral questions will be
Membrane transport revise the previous class before asked in
AND ITS every new class helping them in the class. Problems will
Thermodynamics of
transport cleared, if any.Videos will be ability. Class tests will
shown in the class for a better be conducted for internal
understanding of the concepts. assessment.
Teaching will be conducted both
power
69
PAPER III:PRACTICAL IV
Course objective:
The course aims to develop skills of performing basic microbiological practicals, to develop
familiarity with biochemical laboratory techniques, and to introduce students to various practical
aspects of membrane biology.

1. Preparation of stains andreagents
2. Preparation of various culturemedia
I 3. Preparation of broth andslants 24
4. Sterilization of culture media by autoclavemethod
1. Sterilization of glassware by hot airoven

2. Isolation and propagation ofbacteria
3. Staining of bacteria – Simple staining, differentialstaining, staining
II 24
of spores andcapsules
4. Determination of growth curve ofbacteria
1. Biochemical tests and motility for the identificationof

bacteria.
2. Investigation of the effect of osmotic change on
membranes
III 24
3. Investigate the effect of temperature on membrane
permeability.
4. Investigate the effect of pH change on membrane.
5. Study of osmosis
1. Effect of detergents and other membrane activesubstances
onErythrocytes.
IV 2. Effect of ethanol on beetrootmembrane. 24
3. Effect of lipid composition on the permeability of alipid
monolayer.
TOTAL 96
70
Text books:
1. G.F. Brooks, J.S. Butel, S.A. Morse, J.L. Melnick, E. Jawetz, E.A. Adelberg..Jawetz,Melnick
&Adelberg‘s Medical Microbiology, 27th Edn. Lange Publication.USA
2. P. Cossart, P. Boquet, S. Normark, R. Rappuoli.. Cellular Microbiology, 2ndEdn.American
Society for Microbiology Press.USA
3. A.A. Salyers, D.D. Whitt.. Bacterial Pathogenesis: A molecular approach. AmericanSociety
for Microbiology Press, Washington, DCUSA
4. J. Hacker, U. Dorbindt..Pathogenomics: Genome analysis of pathogenic microbes, Wiley-
VCH.Germany
5. D.H. Persing, F.C. Tenover, J. Versalovic, Y. Tang, E.R. Unger, D.A. Relman, T.J.White..
Molecular Microbiology: Diagnostic Principles and Practice, 2nd Edn. American Society for
Microbiology Press.USA
6. K.E. Nelson, C.M. Williams, N.M.H. Graham.. Infectious Disease Epidemiology: Theoryand
Practice, 3rd Edn.(2013) An AspenPublication.
7. Practical Clinical Biochemistry, ed. Harold Varley, 4th edn. CBS Publishers(.
9. Biochemistry, ed. Plummer 3rd Edn. Tata-McGrawHill,.

Learning
Outcomes
Activity
I Students will learn Chalk and board Discussions, oral

preparation of stains, teachings, video questioning and
reagents, culture media, and presentations of the analytical questions
sterilization methology, will be given to
calculations guidance, students. Students
handlingequipments
71
II Students will learn isolation Chalk and board Discussions, oral
of bacteria, staining and teachings, questioning and
growth pattern calculations guidance, analytical questions
video presentation of will be given to
handlingequipments
III Students will able to identify Chalk and board Discussions, oral
bacteria by biochemical teachings, questioning and
testand motility; they will calculations guidance, analytical questions
also learn to run SDS-PAGE video presentation of will be given to
handlingequipments
IV Students will learn the effect Chalk and board Discussions, oral
of detergents on erythrocyte, teachings, questioning and
effect of ethanol on beetroot calculations guidance, analytical questions
membrane and Effect of lipid video presentation of will be given to
composition on the the methology, students. Students
permeability of a lipid conversion of moles, ‗hands on‘ learning
monolayer molar, mg, g, L etc, will be regularly
handlingequipments
72
DISCLIPLINE SPECIFIC ELECTIVE
73
M.SC. BIOCHEMISTRY (CBCS STRUCTURE)
DISCIPLINE SPECIFIC ELECTIVE (DSE) COURSES
Discipline Specific Elective Course – I (DSE- I): Bioanalytical Techniques,
Code:BCH154D101Credits: 3-1-0-4, Total Hours: 64
Course objective:
The objective of the course is to provide students with a broad understanding of the principles of
bioanalytical instrumentation and to provide an appreciation of their uses.
Course content:
Electrophoretic techniques: Principles of electrophoretic separation.

Continuous, free, zonal and capillary electrophoresis, different types of
electrophoresis including paper, cellulose, pulse field gel
electrophoresis.
I Spectroscopy: Concepts of spectroscopy, Visible and UV spectroscopy, 16
Laws of photometry.
Beer-Lambert‘s law, Principles and applications of colorimetry.
Chromatography: Principles of partition chromatography, paper, thin
layer, ion exchange and affinity chromatography, gel permeation
II chromatography, HPLC, gas chromatography. 16
Centrifugation: Principles of centrifugation, concepts of RCF, different

types of instruments and rotors, preparative, differential and density
III 16
gradient centrifugation, analytical ultra centrifugation, determination of
molecular weights and other applications, sub cellular fractionation
Electronmicroscopy:Light,electron(scanningandtransmission),phase
contrast, fluorescence microscopy, freeze-fracture techniques, specific
IV staining of organelles or markerenzymes. 16
Total 64
74
Text Books:
1. Principles and Techniques of Practical Biochemistry, Keith Wilson and John Walker,
5thedition, 2000.
2. Physical Biochemistry, application to Biochemistry and Molecular Biology, David
Freifelder, 2nd edition,1982.
3. R. F. Boyer, Modern experimental biochemistry, Benjamin Cummings, San Francisco,
3rd ed.,2000.
4. R. F. Boyer, Biochemistry laboratory: modern theory and techniques, Prentice Hall,
Boston, 2nd ed.,2012.
5. D. Harvey, Modern analytical chemistry, McGraw-Hill, Boston, 2000.

LearningActivity
I Students will understand the Marker and board, Students will begiven
working principle andapplications Powerpoint assignment for
of electrophoreticand presentations, student example; oral
spectroscopictechnique interaction questions will be asked
II Students will learn the working Marker and board,

Students will be asked
principle of chromatography and Powerpoint questions; students will
will be familiar with different types presentations, student
be given aresearch
of chromatography interaction problem and asked to
address the
question using
combinationof
techniques;
Experimental
designs.
III Students will learn the working Marker and board, Students will be asked
principleofcentrifugationandwill be Powerpoint questions; students will
familiar with different presentations, student be given aresearch
centrifugation methods as well as interaction problem and asked to
applications address the
question using
combinationof
techniques;
Experimental
designs.
75
IV Students will learn about the Marker and board, Students will be asked
working principles of microscopy, Powerpoint questions; students will
different types of microscope and presentations, student be given aresearch
interaction problem and asked to
their applications
address the
question using
combinationof
techniques;
Experimental
designs.
76
Discipline Specific Elective Course – I (DSE- I): Bioinformatics and biostatistics,
Code:BCH154D101 Credits: 3-1-0-4, Total Hours: 64
Specific Elective Course – III (DSE- III): Biostatistics and Bioinformatics

Course objective:
Theory, Credits: 4, Total Hours: 64
The course is aimed at introducing the application of bioinformatics and statistics in biology. The
course gives an insight into the key concepts and methods used in bioinformatics; and computer
storage, retrieval, analysis, visualization, and distribution of information data related to
biological macromolecules like DNA, RNA and proteins. It provides foundation on statistical
methods to enable students to compute and interpret basic statistical parameters. As an
interdisciplinary field it integrates biology, computer science, chemistry, and statistics together
sequence analysis structure analysis and functional analysis of biological data.

Basics of Bioinformatics: definitions, scope and goal, applications in
computational biology, limitations; Biological database: types of database,
biological database, information retrieval from biological database; Sequence
I alignment: pairwise, multiple, database similarity search, scoring matrices, 16
Molecular phylogenetics: distance based methods, character based methods.
Structural Bioinformatics: proteins and its structure, determination of protein

3 dimensional structure, protein structure visualization, comparison,
secondary and tertiary structure prediction, Genomics and Proteomics:
genome mapping, genome sequence, genome sequence
II assembly,comparativegenomics,FunctionalGenomics:sequencebased 16
approaches, microarray based approach, Proteomics: technology of protein
expression analysis, protein sorting, protein-protein interaction; Computer
Aided Drug Designing (CADD), pharmacophoremodeling.
Statistical Tools: measures of central tendencies and dispersion, concept of
probability and theoretical distributions (bionomial, poisson, and normal
distribution), correlation and regression; univariate and multivariate multiple
III regression, random numbers, sampling methods, 16
randomplotdesign,basicsoftestingofhypothesis,Analysisofvariance (one way
and two way), Students t test, Chi-square test, F-test, and Z- test.
77
Statistical Science and Biological Assay: importance, nature and planning
of bioassays, direct and indirect bioassays, design of experiments by Analysis
of variance and Dose-response analysis.
Analysis of Biochemical Data: application of multiple regressions in
IV epidemiologic and clinical data, study of associations between disease and 16
risk factors, Application of odds ratio, Logistic regression with dichotomous
response variable.
Total 64

LearningActivity
I Students will learn the basics of Students will be asked Students will be asked
bioinformatics, different types of to orally revise the questions; Internal
databases, and distance-based previous class before assessment tests (mid-
every new class term and end-term)will
methods and character-based
helping them in better be conducted.
methods involved in molecular understanding and their
phylogenetics doubts
Cleared if any.
Teaching willbe
power point
presentations
mode
II Students will learn protein‘s Students will be asked Students will be asked
structure, visualization, to orally revise the questions; Internal
comparison and prediction previous class before assessment tests (mid-
understructural bioinformatics;
helping them in better
students will also gain knowledge understanding and their be conducted.
about genomics andproteomics doubts
Cleared if any.
Teaching willbe
power point
presentations
mode
78
III Students will be familiar with Students will be asked Students will be asked
various statistical methods, their to orally revise the questions; Internal
applications and significance previous class before assessment tests (mid-
understanding and their
doubts
Cleared if any.
Teaching willbe
power point
presentations
mode
IV Students will learn various Students will be asked Students will be asked
biological assays, theirimportance to orally revise the questions; Internal
and analysis of biologicaldata previous class before assessment tests (mid-
doubts
Cleared if any.
Teaching willbe
power point
presentations
mode
79
Discipline Specific Elective Course – II (DSE- II): Nutritional Biochemistry,Code:BCH154D201
Credits: 3, Total Hours: 48
Course objective:
This course aims at understanding the food groups, formulation of balanced diets, vitamin types,
humannutrition,andthedisordersthatariseduetonutritionalimbalancesanditsimpactonoverall health,
and gives an introduction tonutrigenomics.
Course content:

Basic concepts: Function of nutrients. Measurement of the fuel values of
foods. Direct and
indirect calorimetry. Basal metabolic rate: factors affecting BMR,
measurement and calculation of
BMR. Measurement of energy requirements. Specific dynamic action of
proteins.
Elements of nutrition: Dietary requirement of carbohydrates, lipids and
I 12
proteins. Biological
value of proteins. Concept of protein quality. Protein sparing action of
carbohydrates and fats.
Essential amino acids, essential fatty acids, and their physiological functions.
Minerals: Nutritional significance of dietary calcium, phosphorus,
magnesium, iron, iodine, zinc and copper.
Vitamins: Dietary sources, biochemical functions, requirements, and
II deficiency diseases associated with vitamin B complex, C and A, D, E & K 12
vitamins.
Malnutrition: Prevention of malnutrition, improvement of diets.
Recommended dietary
allowances, nutritive value of common foods. Protein-calorie
malnutrition. Requirement ofproteins
III 12
and calories under different physiological states- infancy, childhood,
adolescence, pregnancy,
lactation and ageing.
Starvation:Techniquesforthestudyofstarvation.Proteinmetabolismin
prolongedfasting.
IV Obesity: Definition, Genetic and environmental factors leading to obesity. 12
Total 48
80
Text Books:
1. Nutritional Biochemistry. Author, Tom Brody. Edition, 2. Publisher, Harcourt, Braces,

1999.
2. Principles of Nutritional Assessment (2005) Rosalind Gibson. Oxford UniversityPress.
3. Krause‘s Food and Nutrition Care process.(2012);Mahan, L.K Strings, S.E, Raymond, J.
Elsevier‘sPublications.
4. The vitamins, Fundamental aspects in Nutrition and Health (2008); G.F. Coombs Jr.
Elsevier‘sPublications.
5. Principles and Techniques of Practical Biochemistry, Keith Wilson and John Walker,
5thedition, 2000.
6. Physical Biochemistry, application to Biochemistry and Molecular Biology, David
Freifelder, 2nd edition,1982.
Unit No. Course Learning Outcomes Teaching and Assessment

LearningActivity Tasks
I Students will learn the basicconcepts of Students will be asked Students will be
nutritional biochemistry including to orally revise the asked questions;
the nutritional importance of previous class Quiz, internal
biomolecules such as before every new assessment
carbohydrates, proteins, amino class helping them tests will be
acids,lipids in better conducted.
understanding and
their doubts
II Students will gain knowledge cleared, if any.
about different types ofvitamins and Teaching will be
minerals, their nutritional conducted both
importance, and diseases due to their through white
deficiency in humans board mode and
III Students will learn about malnutrition, its power point
prevention, recommended dietary presentation
allowance, importance of protein under mode.
different physiological state
IV Students will learn the process of protein
metabolism under prolonged
fasting conditions and causes of obesity
81
Discipline Specific Elective Course –II (DSE-II): Neurobiochemistry Code:BCH154D201

Course objective:
This paper provides a basic understanding of the nervous system, Structure and function
relationship and integration of the nervous tissue networking and insights in to neurotransmission
Course contents:
Neuron- Neurocellular anatomy, neural membrane, classification of

I neuron,nervefibers,axonaltransport,neuralgrowth,neuroglia,nervous 12
system, blood brain barrier, cerebrospinalfluid.
Neuronal signaling -Membrane potentials, ion channels, recording

neuronal signals, ionic basis of resting potential and action potential,
propagation of action potential.
Synaptic transmission- Synapse, Electrical synapse transmission,
II chemical synaptic 12
transmission, Synaptic transmitter release, synaptic potentials, synaptic
delay, synaptic plasticity, molecular mechanism of synaptic
transmission, myoneural junction.
Neurotransmitters- Chemistry, synthesis, storage, release, receptors and
III function- acetyl choline, catecholamines, serotonin, histamine, 12
glutamate, asparatate, GABA, glycine, neuropeptides, nitric oxide.
Neural processing and neurodegenerative disorders-Learning and

IV memory, neurochemical basis of drug abuse, neurodegenerative 12
disorders, Parkinson‘s disorder, Alzheimer‘s disorder,
Amyotrophic Lateral Sclerosis, Senile Dementia
Total 48
82
.
Text books:
1. Arthur C. Guyton and John E Hall, Text book of medical physiology 11th Edition;2006
2. David Nelson and Michael Cox, Lehninger Principles of Biochemistry, 4th edition;2005
3. Bruce Alberts, Alexander Johnson, Juliana Lewis, Martin Raff, Keith Roberts and Peter
Walter, Molecular biology of the cell, 4th Edition;2004
4. Gordon Shepherd, Neurobiology, 3rd Edition;1994
5. MarkFBear,BarryWConnorsandMichaelAParadiso,Neuroscience:Exploringthebrain,4th
Edition; 2015

LearningActivity
I Students will learn about neurons, Students will be asked Oral questions will be
nerve fibres, nervous system and to orally revise the asked in the class.
blood brain barrier previous Problems will be
assigned to test
II Students will understand different class before every student‘s
phenomena involved in neuronal new class helping
signaling and synaptic them in better analytical ability.
transmission Class testswill
III Students will learn about understanding and be conducted for
neurotransmitters including its their doubts internal
synthesis, storage, release,binding cleared, if any. assessment.
with receptors, andtheir Videos will be
mechanisms of action shown in the class
IV Students will gain knowledge for a better
about various diseases triggered
understanding of
due to disruptions of neural
processing and etiology of the concepts.
neurodegenerative diseases Teaching will be
conducted both
through white board
mode and power
point presentations
mode
83
Discipline Specific Elective Course – III /IV/V: Environmental Science
Code:BCH154D301/302/303
Course objective:
This course introduces students to environment concerns. Students are expected to learn about
environment, factors affecting it, environmental ethics and its protection through lectures,
presentations, documentaries, and field visits.
Course content:
The multidisciplinary nature of environmental studies

I Definition, scope and importance. Need for public awareness. 8
Natural resources: renewable and non-renewable resources
Natural resources and associated problems. Forest resources : Use and

over-exploitation,deforestation,casestudies.Timberextraction,mining,
damsandtheireffectsonforestsandtribalpeople.Waterresources:Use and
over-utilization of surface and ground water, floods, drought, conflicts
over water, dams benefits and problems. Mineral resources : Use and
exploitation, environmental effects of extracting and using mineral
resources, case studies. Food resources : World food problems, changes
II caused by agriculture and over-grazing, effects of modern agriculture, 24
fertilizer-pesticide problems, water logging, salinity, case studies. Energy
resources : Growing energy needs, renewable and non- renewable energy
sources, use of alternate energy sources, Casestudies. Land resources :
Land as a resource, land degradation, man induced landslides, soil
erosion and desertification. Role of an individual in conservation of
natural resources. Equitable use of resources for sustainable lifestyles.
Environmental pollution, definition, Causes, effects and control

measures
Air pollution, Water pollution, Soil pollution, Marine pollution, Noise

pollution, Thermal pollution, Nuclear hazards. Solid wastemanagement
III : Causes, effects and control measures of urban and industrial wastes. 18
Role of an individual in prevention of pollution. Pollution case studies.
Disaster management : floods, earthquake, cyclone and landslides.
84
Bio-diversity and its conservation, introduction, definition
Genetic,speciesandecosystemdiversity.Biogeographicalclassification
ofIndia.Valueofbiodiversity:consumptiveuse,productiveuse,social,
IV ethical, aesthetic and option values. Biodiversity at global, national and 16
local levels. India as a maga-diversity nation. Hot-spots ofbiodiversity.
Threatstobiodiversity:habitatloss,poachingofwildlife,man-wildlife
conflicts. Endangered and endemic species of India. Conservation of
biodiversity : In-situ and Ex-situ conservation ofbiodiversity
Total 64
Text Books
1. ResearchinEducation(1992)6thed.,Best,J.W.andKahn,J.V.,PrenticeHallofIndiaPvt.Ltd. ISBN-
978-81-203-3563-9.
2. AttheBench:ALaboratoryNavigator(2005)Barker,K.,ColdSpringHarborLaboratoryPress (New
York), ISBN: 978-087969708-2.42
3. Research Methodology - Methods and Techniques (2004) 2nd ed., Kothari C.R., New Age
International Publishers. ISBN –81-224-1522-9
4. ResearchMethodology:AStepbyStepGuideforBeginners(2005)2nded.,KumarR.,Pearson
Education. ISBN:978-1-4129-6467-8.
5. Biostatistics: A Foundation for Analysis in the Health Sciences (2013) 10th ed., Daniel W.W.,
John Wiley and Sons Inc. ISBN-13: 978-1118302798 ISBN-10:1118302796
6. StatisticsattheBench:AStep-by-StepHandbookforBiologists(2010)Bremer,M.andDoerge, R.W.,
Cold Spring Harbor Laboratory Press (New York), ISBN: 978-0-879698-57-7.

LearningActivity
I Students will learn the basics of Students will be asked Oral questions willbe
environmental science including to orally revise the asked; students will be
definitions, scope and importance previous class before asked to discuss the
every new class topic. Quiz,
85
II helping them in better internalassessment
Students will understand about the
various renewable and non- understanding and their tests will be conducted.
renewable natural resourcesdoubts
cleared, if
including forest resources, food
any. Teaching
resources, mineral resources, willbe
energy resources and land conducted both through
resources white board mode and
power point
presentations
III Students will understand and
mode
realize the seriousness of
environmental pollution, and its
causes, effects and control
measures
IV Students will understand the

importance of biodiversity and
will learn about different threatsto
biodiversity in terms of habitat
loss, poaching of wildlife, man-
wildlifeconflicts
86
Discipline Specific Elective Course – III/IV/V : Research Methodology
Code:BCH154D301/302/303
Course objective:
The objective of the course is to:
 familiarize students with basics of research and the researchprocess

 provide an introduction to research methods and reportwriting
 give insight into various kinds of research design andsampling
 enable students to evaluate data through statisticalanalysis
Course content:
Introduction to research methodology, objectives and motivation in

I research. 8
Defining the Research Problem .Selecting and defining a research
problem, Reviewing and conducting literature search, developing a
II research plan. 18
Designing of Experiment Different experimental designs – single and

III multifactorial design, Making measurements and sources of error in 20
measurements, Methods of data collection and record keeping.
Data Processing and Statistical Analysis .Processing operations,
tabulation, and graphical representation, Statistics in research: Concepts
of sample and population, Measure of central tendency, dispersion,
asymmetry (skewness, kurtosis), Normal distribution (p-value),
IV Statistical tests and hypothesis (Standard error, t-test, chi-square test), 18
and regression analysis, Report writing, Writing a research paper -
abstract, introduction, methodology, results and discussion.
Total
64
87
Text Books:
1. ResearchinEducation(1992)6thed.,Best,J.W.andKahn,J.V.,PrenticeHallofIndiaPvt.Ltd. ISBN-
978-81-203-3563-9.
2. AttheBench:ALaboratoryNavigator(2005)Barker,K.,ColdSpringHarborLaboratoryPress (New
York), ISBN: 978-087969708-2.42
3. Research Methodology - Methods and Techniques (2004) 2nd ed., Kothari C.R., New Age
International Publishers. ISBN –81-224-1522-9
4. ResearchMethodology:AStepbyStepGuideforBeginners(2005)2nded.,KumarR.,Pearson
Education. ISBN:978-1-4129-6467-8.
5. Biostatistics: A Foundation for Analysis in the Health Sciences (2013) 10th ed., Daniel W.W.,
John Wiley and Sons Inc. ISBN-13: 978-1118302798 ISBN-10:1118302796
6. StatisticsattheBench:AStep-by-StepHandbookforBiologists(2010)Bremer,M.andDoerge, R.W.,
Cold Spring Harbor Laboratory Press (New York), ISBN: 978-0-879698-57-7.

LearningActivity
I Students will learn about the Students will be asked Oral questions willbe
basics of research methodology to orally revise the asked; students will
encompassing its objectives and previous class before be asked to discuss
every new class
motivation in research the topic.
helping
II Students will learn how to select them in better
and define a research problem;
doubts cleared, if any.
importance of review literature Teaching will be
III Students will learn about different black board mode and
experimental designs, methods of power point
data collection and about record presentation mode.
keeping
IV Studentswilllearnanalysisofdata
applying various statistical
methods and gain knowledge
about different ways to represent
these analyzeddata
88
Discipline Specific Elective Course – III/IV/V : Genetics for Biologist
Code:BCH154D301/302/303
Course objective:
To provide an understanding of:
 DNA replication in prokaryotic and eukaryoticorganisms

 transcription and translation process in prokaryotic and eukaryotic organisms
 learn about the Mendelian Genetics and its principles ofheredity
 to focus on the basic principles of genetics incorporating the concepts of classical,
molecular and populationgenetics.
 mechanisms involved in regulation of gene expression in prokaryotic and eukaryotic
organisms
 Course content:

Griffith‘s transformation experiment, Avery‘s transformation experiment,
I Hershey and Chase‘s bacteriophage experiment, RNA as genetic material. 16
Semi-conservative DNA replication, Meselson-Stahl experiment, DNA
polymerase, DNA replication in prokaryotes, Replication initiation,
semidiscontiniousreplication,rollingcyclereplication,DNAreplication in
eukaryotes, replicons, initiation, replicating ends ofchromosomes.
Transcription initiation in prokaryotes, elongation of RNA chain, termination
of RNA chain. Transcription in eukaryotes, transcription of protein coding
genes, production of eukaryotic mRNAs, Splicing of mRNAs.
Nature of the genetic code, transfer RNA, ribosomes, initiation of translation,
II elongation of the polypeptide chain, termination of translation. 16
Mendel‘s principle of segregation, Mendel‘s principle of independent
assortment, Mendelian genetics inhumans.
Multiple alleles, ABO blood groups, Drosophila eye colour, incomplete
dominance, codominance, maternal effect, extranuclear genes, extranuclear
III inheritance, chromosome theory of inheritance, sex chromosomes, sex linkage, 16
sex linkedtraits
Lac operon of E.coli, lactose as a carbon source in E.coli, molecular
detailsoflacoperonregulation,trpoperoninE.coli,regulationofthetrpoperon,
regulation of gene expression in phagelambda.
Transcription initiation by activators, transcription initiation by repressors,
IV regulation of gene activity by histones and chromatin remodeling, gene 16
silencing by DNA methylation and regulatory RNAs.
Total 64
89
Text Book
1. FundamentalsofBiochemistry:Lifeatthemolecularlevel,4thEdition.D.Voet,J.G.Voet and
W.Pratt(2012).
2. Principles of Biochemistry, 4Th Edition. Nelson, D.L. and M.M. Cox (2005). 3.
Biochemistry, 5th Edition. Jeremy M. Berg, John L.Tymoczko and LubertStryer(2002).
3. iGenetics, 3rd Edition, Peter JRussel.
4. Molecular Biology of the Gene, Watson et al . 7thedition.
5. Benjamin Lewin, Genes XI. Jones & Bartlett Learning; 11th edition (2012).

Learning Activity
I Students will learn about various early Students will be asked Oral questions will be
experiments with regard to DNA, semi- to orally revise the asked in
conservative model of DNA replication, previous class before the class. Problems will
differences in the process of DNA every new class helping be
replication between prokaryotes and them in better assigned to
eukaryotes understanding and their test student‘s
II Students will understand how DNA doubts cleared, if any. analytical ability.
converts to mRNA and later Videos will be shown in Class tests will
mRNAconvertstoproteinthrough learning the class for a better
the transcription and translation process understanding of the be conducted
respectively; they will also learn the concepts. for internal
cellular mechanisms that exist to regulate Teaching will be assessment.
theseprocesses conducted both through
III Students will understand the genetic white board
inheritance through learning the mode and power point
Mendelian‘s presentations
mode
principles and different entitiesor traits
that exhibit genetic inheritance
IV Students will understand the regulation
of gene expression at transcription and
translation level through learning
different model system in prokaryotes
and eukaryotes
90
Discipline Specific Elective Course – III/IV/V : Microbial Biochemistry
Code:BCH154D301/302/303
Course objective:
The Medical Microbiology course has been formulated to impart basic and medically relevant
information on the microbes. The microbial structure, growth and development, methods,
androle of sterilization in the context of study of microbes are included. The pathogenic microbes
and the diseases caused by them are included to broaden the perspective of the subject. This
course will also focus on mechanisms of microbial pathogenesis and the host response, and the
scientific approaches that are used to investigate theseprocesses.
Course content:
Modu Topics / Course content Periods

les
Fundamentalconcept:Historyofmicrobiologywithspecialemphasison
contributionofLouisPasteurandRobertKochinMedicalMicrobiology. Major
Divisions of life- Domains, Kingdoms, Classical and Molecular methods of
assessing microbial phylogeny- molecular chronometer, phylogenetic trees,
rRNA, DNA and proteins as indicator of phylogeny. Requirements for microbial
I growth, growth factors, culture media- synthetic and complex, types of media. 16
Obtaining Pure Cultures, Preserving Bacterial Cultures, Growth Curves and
generation time, Control of microbial growth, general concept of effect
ofenvironmental factors on growth ofmicrobes.
Bacterial cells-fine structure and function: Size, shape and arrangement of
bacterial cells; Cell membrane, cytoplasmic matrix, inclusion bodies
II (egmagnetosomes),nucleoid,UltrastructureofGram+veandGram–ve bacterial cell 16
wall, Pili, Capsule, Flagella and motility,endospore.
Host-Pathogen relationship in the infectious diseases: Relationship between
Normal microbiota and host, Opportunistic microorganisms, nosocomial
infections, Development and spread of infectious disease: invasion, pathogen,
III 16
parasite, pathogenicity, virulence, carriers and their types. Routes, mechanisms
of invasion and establishment of infection.
Microbial diseases: Respiratory tract infections (Tuberculosis); Gastrointestinal
tract infections including Diarrhea caused by Salmonella and E.coli,
IV staphylococcal food poisoning. Clinical symptoms and life cycle of Candida 16
albicans and Plasmodium.General
lifecycleofavirus,structure,envelopedandun-envelopedviruses, plaque assay,
growth curve, classification based on genetic material and detail study of
influenza and HIV virus with curative agent. Virods, virusoids and prions.
Total 64
91
Text Books:
1. Microbiology: An Introduction, 9th edition (2008), Gerard J. Tortora, Berdell R. Funke,

Christine L. Case; Benjamin Cummings. ISBN-13:978-0321733603.
2. Prescott, Harley, and Klein's Microbiology, 8th edition, (2011), Joanne M. Willey, Linda M.
Sherwood, Christopher J. Woolverton, McGraw Hill International. ISBN-13:978-0071313674.
3. Bailey and Scotts Diagnostic Microbiology, 12th edition (2007), Betty A. Forbes, Daniel F.
Sahm and Alice S. Weissfeld; Mosby Elsevier Publishers, ISBN-13: 978-0808923640.
4. Microbiology, 6th edition (1993), Pelczar, Chan and Krieg; McGraw Hill International, ISBN-
13: 978-0070492585.
5. BrockBiologyofMicroorganisms,13thedition(2010),MichaelT.Madigan,JohnM.Martinko,
David Stahl and David P. Clark, Pearsons, Benjamin Cummings, ISBN-13: 978-0321649638.
6. Microbiology: A Laboratory Manual, 10th edition, (2013), James Cappuccino and Natalie
Sherman, Benjamin Cummings. ISBN-13:978-0321840226.

LearningActivity
I Students will learn about the Teaching will be Students will be
contributions of Louis Pasteur and conducted both through evaluated
Robert Koch in Medical white board mode and through class
power point presentations discussion,
Microbiology, different methods to
mode assignments and tests.
assess microbial phylogeny and Students willbe
different bacterialcultures assigned various
topics andwill
II Students will learn about size,
be asked to deliver a
different shapes, cellular organelles, powerpoint presentation
and ultrastructure of bacterial cells on the
III Students will learn about
development and spread of infectious assigned topics
diseases; they willalso
learn about routes, mechanismsof
invasion and
establishmentofinfection
IV Students will understand about

pathogenicity of bacteria and viruses
through learning etiology
ofvariousdiseasescausedbythem
92
Discipline Specific Elective Course – VI/VII/VIII: Biochemistry of Common Disorders
Code:BCH152D401/402/403
Course objective:
Theobjectiveistoprovideknowledgeaboutcommonlifestyledisorderswithdetailedinsightinto two
major killers: Cancer and Cardiovasculardiseases.
Course content:
Human Physiology: Introduction and brief description of cells, tissues and

organs, their functions; Body fluids and their composition. Introduction to
molecules as building blocks. Definition and differentiation of disease and
disorder, types and causes. Relation between food, environment and illness.
I Analysis of variousbiochemical parameters in body fluids and specific tissues 16
during disorders, diseases andforensics.
DiagnosticTechniques:Collectionandstorageofbiologicalsamplesfor clinical
use. Commonly used tests for diagnosis of various diseases and their
II interpretation. Blood analysis: Total blood count including ESR, Total serum 16
proteins and their fractions. Blood glucose (GTT) (Fasting andpost-
prandial),serumlipidfraction–cholesterol,triglyceride,LDL
andHDL,bloodurea,andserumcalcium.Urine:Creatinine,Glucose and protein
(albumin). Enzymes: SGPT, SGOT and isoenzymes as markers in various
disorders and diseases.
Diseases and Disorders (common occurrence): Aetiology; classification
(ifany);causativefactors;incidence,symptomsandbiochemicalaspects
III andmarkersfor-identification,monitoring,preventionandinterventions; and 16
nutritional aspects, overweight andobesity.
Cardiovascular disease: Diabetes, Gall bladder & Hepatitis, (A, B, and C),
Cirrhosis, alcoholic liver disease, Gall stones, pancreatitis- Causes, Prevention
and dietary management.
Renal disease: Nephrotic syndrome, Acute and Chronic renal failure-

IV diagnostic procedures and dietary management. 16
CancerandHIV/AIDS:Biochemistryofcarcinogenesis,types,stagesof cancer,
diagnosis and existing medicines. Biochemistry of HIV infection, ART and
socialissues.
Total 64
93
Text Books:
1. Biochemistry; Donald Voet, Judith G. Voet, 4th Edition, John Wiley and sons(2010).
2. Lehninger-PrinciplesofBiochemistry;DavidL.NelsonandMichaelM.Cox,6thEdition,
W. H. Freeman (2013). 46
3. Biochemistry- The Chemical Reactions of Living Cells; David E. Metzler, 2nd Edition,
Academic Press(2001).
4. Outlines of Biochemistry; Eric E. Conn, Paul K. Stumpf, George Breuning, Roy H. Doi,
5th Edition, John-Wiley and sons(2009).
5. Biochemistry- The Chemical Reactions of Living Cells; David E. Metzler, 2nd Edition,
Academic Press(2001).
6. Hawk‘s Physiological Chemistry, ed. Oser, 14th Edn. (1976), Tata-McGrawHill.
7. Fundamentals of Practical Biochemistry. Mohanty and Basu, BI Publications, India.2002.
8. Clinical Biochemistry, 2nd Edn. W J Marshall, F I Biol and S K Bangert. Elsevier
HealthMosby Saunders. United States of America. ISBN:9780443101861.
Unit Course Learning Teaching and Assessment

No. Outcomes LearningActivity Tasks
I Students will learn about various Teaching will be conducted Oral questions willbe
compositions of human physiology, both through white board asked; students will
mode and power point be asked to discuss
different diseases and disorders, and
presentation
various biochemical parameters the topic. Quiz,
mode.
during these diseases anddisorders internalassessment
tests will be
conducted.
II Students will learn methods and
various criteria for the collection and
storage of biological samples;
students will also gain knowledge on
various diagnostics techniques for
detection biochemical parameters and
markers in blood under
pathophysiological
conditions
III Students will gain knowledge about
various aspects of diseases and
disorders that commonly occur in
human
IV Students will learn about various
94
cardiovascular diseases, renal diseases;
students will learn biochemical
phenomena of cancer and AIDS
95
Discipline Specific Elective Course – VI/VII/VIII : General Pharmacology
Code:BCH154D401/402/403
Course Objective: The main purpose of the subject is to understand what drugs do to the livingorganisms and
how their effects can be applied to therapeutics. The subject covers theinformation about the drugs like,
mechanism of action, physiological and biochemicaleffects (pharmacodynamics) as well as absorption,
distribution, metabolism, and excretion(Pharmacokinetics) along with the adverse effects, clinical uses,
interactions, doses,Contraindications and routes of administration of different classes of drugs.
Detailed Syllabus
Modules Topics (if applicable) & Course Contents Periods
General Pharmacology
a. Introduction to Pharmacology- Definition, historical landmarks, and scope of
pharmacology, nature and source of drugs, essential drugs concept and routes of
drug administration, Agonists, antagonists (competitive and non-competitive),
08 hours
I spare receptors, addiction, tolerance, dependence, tachyphylaxis, idiosyncrasy,
allergy.
b. Pharmacokinetics- Membrane transport, absorption, distribution, metabolism,
and excretion of drugs. Enzyme induction, enzyme inhibition, kinetics of
elimination
General Pharmacology
a. Pharmacodynamics- Principles and mechanisms of drug action. Receptor
theories and classification of receptors, regulation of receptors. drug receptors
interactions signal transduction mechanisms, G-protein–coupled receptors, ion
channel receptor, transmembrane enzyme linked receptors, transmembrane JAK-
STAT binding receptor and receptors that regulate transcription factors, dose
II response relationship, therapeutic index, combined effects of drugs and factors 12 Hours
modifying drug action.
b. Adverse drug reactions.
c. Drug interactions (pharmacokinetic and pharmacodynamic)
d. Drug discovery and clinical evaluation of new drugs -Drug discovery phase,
preclinical evaluation phase, clinical trial phase, phases of clinical trials and
pharmacovigilance
Pharmacology of drugs acting on peripheral nervous system
a. Organization and function of ANS.
b.Neurohumoraltransmission,co-transmission and classification of
neurotransmitters.
III c. Parasympathomimetics, Parasympatholytics, Sympathomimetics, 10 Hours
sympatholytics.
d. Neuromuscular blocking agents and skeletal muscle relaxants (peripheral).
e. Local anesthetic agents.
f. Drugs used in myasthenia gravis and glaucoma
Pharmacology of drugs acting on central nervous system
IV a. Neurohumoral transmission in the C.N.S.special emphasis on importance of 15 Hours
various neurotransmitters like with GABA, Glutamate, Glycine, serotonin,
96
dopamine.
b. General anesthetics and pre-anesthetics.
c. Sedatives, hypnotics and centrally acting muscle relaxants.
d. Anti-epileptics
e. Alcohols and disulfiram
f. Psychopharmacological agents: Antipsychotics, antidepressants, anti-anxiety
agents, anti-manics and hallucinogens.
g.Drugs used in Parkinsons disease and Alzheimer‘s disease.
h. CNS stimulants and nootropics.
i. Opioid analgesics and antagonists
j. Drug addiction, drug abuse, tolerance and dependence.
Total 64
Text Books:
1.Rang, H. P., Henderson, G., Flower, R. J., Dale, M. M. (2015). Rang&Dale's Pharmacology. 8th
edition.United Kingdom. Churchil LivingstoneElsevier
2. Katzung B. G., Masters S. B., Trevor A. J.(2013). Basic and clinical pharmacology, 11 th edition. New
Delhi. Tata McGraw-Hill
3.Laurence L. Brunton et al.(2017).Goodman and Gilman‘s, The Pharmacological Basis of Therapeutics.
13th edition. Mcgrow hill education.
4. Marry Anne K. K.et al. (2008). Applied Therapeutics, The Clinical use of Drugs. 9th edition .The Point
Lippincott Williams &Wilkins
5. Mycek M.J, Gelnet S.B and Perper M.M. (2018). Lippincott‘s Illustrated Reviews-Pharmacology. 7th
edition.
6. Tripathi, K. (2016). Essentials of Medical Pharmacology. 8th edition. India: Jaypee Brothers, Medical
Publishers Pvt. Limited
Reference Book:
7.Sharma. H. L., Sharma, K. K. (2017). Sharma & Sharma's Principles of Pharmacology. India: Paras
Medical Publisher.
8.Stitzel, R. E. (2004). Modern Pharmacology with Clinical Applications. 6th edition. United
Kingdom: Lippincott Williams & Wilkins.
9. Ghosh MN.(2015).Fundamentals Of Experimental Pharmacology. India: Hilton & Company.
Teaching Learning Process and Assessment Methods

Unit Course Learning Teaching and Learning Assessment Tasks
No. Outcomes Activity
I Understand the Regular chalk and board MCQ based assignments will
pharmacological actions of teaching along with PPT be given to students to check
different categories of presentations. Class discussions their understanding of the
drugs on syllabus topics will be subject.
performed.
II Students will be ableto Teaching will be conducted both Oral questions will be asked
explain the mechanism of through black board mode and in the class. Students will be
drug action at organ power point presentation mode given to prepare power point
system/sub cellular/ presentation on the assigned
macromolecular levels. topics related to the class
teachings.
97
III Apply the basic Teaching will be conducted both Problem solving assignments,
pharmacological through black board mode and regular question answer
knowledge in the power point presentation mode. sessions, MCQs and unit-test
prevention and treatment Software‘s/ Videos will be for internal assessment
of various diseases and issued to demonstrate animal
students will be able to experiment.
observe the effect of drugs
on animals by simulated
experiments
IV Appreciate correlation of Appropriate mix of chalk and Internal assessment tests will
pharmacology with other board teaching as well as use of be conducted, – presentations
bio medical sciences Power point presentations for will be assessed along with
clarity of concepts with practical assessment.
reactions, Practical
demonstration will be given.
98
Discipline Specific Elective Course – VI/VII/VIII :Biomaterials
Code:BCH154D401/402/403
Course objective:
Biomaterials restore the body of a person back to normalcy following any trauma or disease. The
papercomprisesoftopicswhichdescribestheproperties,synthesisandapplicationofbiomaterials.
Course content:
Introduction to biomaterials: classification, chemistry and characterization of
biomaterials. The state of the art of biomaterials and
thechallenges.Disciplinesinvolvedinbiomaterialsscienceandthepath from a need
to a manufactured medical device. Material selection requirements for
biomaterials – metals, composites, ceramics and polymers. Tissue environment
of the implanted biomaterial: unit cell processes. Tissue responses to implants.
I 16
Nanomaterials: fullerenes, carbon nanotubes, nanomembranes. Synthesis of
bio-materials, Characterization of chemical, physical, mechanical properties,
visco elasticity, end group analysis, determination of molecular weight of a
polymer.
Biocompatibility: biocompatibility of bio-materials, wound-healing process,
body response to implants, blood compatibility. Tests to assess
biocompatibility of a polymer, modifications to improve biocompatibility.
II 16
Reactions of biomaterials with cellular and extra cellular components.
Modified biomaterials: biodegradative biomaterials, bioactive polymers and

biosynthetic polymers, inert biomaterials, genetically engineered biomaterials
III 16
Applications of biomaterials: tissue replacement implants, acute wound

healing, blood clotting, chronic wound healing and foreign body response. soft-
IV tissue replacements, sutures, surgical tapes, adhesive, percutaneous and skin 16
implants, maxillofacial augmentation, blood
interfacingimplants,hardtissuereplacementimplants,internalFractures
fixation devices, joint replacements, artificial organs, artificial heart,
prosthetic cardiac valves, limb prosthesis, externally powered limb, prosthesis,
dental implants, other applications.
Total 64
99
Text Books:
1. Sujata V. Bhat, Biomaterials , 2nd edition, Narosa Publishing House, New Delhi,
2006.
2. Buddy D. Ratner, B. D. Ratner,Allan S. Hoffman, Biomaterials Science: An
Introduction To Materials In Medicine, 2nd Edition(2004) Publisher: AcademicPress.
3. Fred W.Billmeyer, Text book of Polymer Science. 3rd edition John Wiley and sons
Publications.
Unit No. Course Learning Outcomes Teaching Assessment

and LearningActivity
Tasks
I Students will learn about basics of Students will be Students

biomaterials covering its characteristics, asked to orally will be
classifications, and chemistry; they will also revise the previous evaluated
class before every through
learn about material requirements, tissue
new class helping class
response to implants, and synthesis them in better discussion,
ofbiomaterials understanding and assignments
II Students will learn how to test the their doubts and tests.
biocompatibility of biomaterials, cleared, if Students willbe
modifications to improve biocompatibility, any. assigned
Teaching willbe various
reactions of biomaterials with cellular and
conducted both topics andwill
extracellular components through white be asked to
III Students will learn about variousavailable board mode deliver a
modified biomaterials, andpower point powerpoint
the processes of modification presentations presentation on
the assigned
mode topics.
IV Students will understand the importance of
biomaterials through learning the various
applications of biomaterials in
several areas
100
Discipline Specific Elective Course –VI/VII/VIII: Genetic Engineering
Code:BCH1545D401/402/403
Course objective:
ThiscourseaimstogiveaninsightintothedirectmanipulationofDNAtoalterthecharacteristics of an
organism in a particular way. It envisages concepts, mechanisms, biological designs, functions
and evolutionary significance of genetic modification or manipulation in special organisms and
discusses the recent advance in recombinant DNAtechnology.
Course contents:
Scope to genetic engineering: Introduction to Genetic Engineering and

Biotechnology. Enzymes as Tools for Genetic Engineering: Restriction
Enzymes, Restriction-Modification System, DNA-modifying enzymes,
T4 and E. coli DNA Polymerase (Klenow), DNA-methylase,
I Polynucleotide Kinase, DNA-ligase, Taq DNA polymerase, Reverse 16
Transcriptase,T7andT3RNApolymerases.VehiclesforDNAcloning:
Plasmid DNA vectors, bacteriophage lambda-derivedvectors.
Recombination and cloning: DNA (Gene) cloning, recombinant DNA,
cDNA library, genomic library. Isolation of gene from gene library.
Screening and identification of recombinant DNA clone from gene
library.ExpressionofrecombinantproteinfromaDNAcloneinbacteria
II 16
andpurificationoftheprotein.Someexamplesoftheusefulrecombinant
proteins: Insulin, Streptokinase, enzymes, antibodies,vaccines.
Recentadvancesingenetechnology:PolymeraseChainReaction(PCR) and
Site-directed, Restriction enzyme digestion. Transgenic animals,
Ligation, Cloning, Transformation, Calculation of transformation
efficiency. Mutagenesis. Recent trends in Gene technology. Gene
III 16
Targeting:Knock-insandKnock-outs.TargetedGenomeEditing:ZFNs,
TALENs, CRISPRsetc.
DNA Sequencing and Genome Analysis, Model Genomes. Human
Genome Project and Human Genome Sequences. Applications of
IV Genetic Engineering and Biotechnology in agriculture, medicine and its 16
economic and social implications, Ethical precautions.
Total 64
101
Text Books:
1. Primrose, S.B. and Twyman, R. (2006) Principles of Gene manipulation and Genomics (7th
edition) BlackwellPublishing.
2. Nicholl, D.S.T. (2008) An introduction to Genetic Engineering (3rd edition) Cambridge
UniversityPress.
3. Watson, J.D. (2006) Recombinant DNA (3rd edition) Cold Spring Harbor LaboratoryPress.
4. Brown, T.A. (2001) Gene Cloning and DNA Analysis: AnIntroduction.
5. A PBS Documentary entitled, ―Playing God‖ [History of GeneticEngineering
Unit No. Course Learning Teaching and Assessment Tasks

Outcomes LearningActivity
I Students will learn about Students will be asked to Oral questions will be asked
thescope of genetic orally revise the previous in
engineering along with class before every new the class. Problems will be
various enzymes that are class helping them in assigned to test
widely used in the process better understanding and student‘s
of genetic engineering their doubts cleared, if analytical ability.
II Students will learn the any. Class tests will
process of making gene Videos will be shown in
library, screening of gene the class for a better be conducted for
library, expression and understanding of the internal
purification of protein concepts. assessment.
encoded by a cloned DNA Teaching will be
and some useful conducted both through
recombinant proteins white board mode and
III Students will learn about power point presentations
various techniques mode
involved in genetic
engineering and gene
targeting methods
IV Students will learn about
HumanGenom Project and
applicationsof genetic
engineering and
biotechnology in
differentfields
102
103
104

PG Biochemistry Syllabus

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PG Biochemistry Syllabus

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ROYAL SCHOOL OF BIO - SCIENCES

Learning Outcomes based Biochemistry Postgraduate

Learning Outcomes-based Approach to CurricularPlanning

Nature and extent of the M.Sc. (Hons.)Programme:

Aims of Master’s degree (Honours) programme inBiochemistry:

Qualification descriptors for a Master’s degree inBiochemistry:

Theprogrammewillstrengthenthestudent‘s ability to comprehend

Programme Learning Outcomes (M.Sc. Hons. inBiochemistry)

2. Personal and BehaviouralCompetence

3. Entrepreneurial and socialcompetence

To develop critical thinking, reasoning, interactive

Toexpressacquiredknowledge,skillsandattitudes,case-basedlearning(CBL)canbeusedwhere students can be

The assessment of learners‘ achievement in M.Sc.(Hons.) Biochemistry will be aligned withthe

Assessment priorities: University will prioritize formative assessments (in-semester activities

Structure of M.Sc. (Honours) Biochemistry curriculum

Lecture (L): One hour of teaching per week in a semester is 1 credit

SL. NO. CONTENTS PAGE NOS

1 SCHEME FOR EVALUATION 9

2 PROGRAMME STRUCTURE 10-12

3 SYLLABUS FOR 1ST SEMESTER 14-25

4 SYLLABUS FOR 2ND SEMESTER 29-37

5 SYLLABUS FOR 3RD SEMESTER 39-46

6 SYLLABUS FOR 4TH SEMESTER 48-72

7 DISCLIPLINE SPECIFIC ELECTIVE 74-102

Continuous Evaluation: 15% Continuous Evaluation: 25% Continuous Evaluation: 15%

1st semester 2nd semester

S Subject Names of L T P C TC S Subject Names L T P C T

1 BCH154 Chemistry 3 1 0 4 4 1 BCH154 Enzymolo 3 1 0 4 4

2 BCH154 Cell 3 1 0 4 4 2 BCH154 Molecula 3 1 0 4 4

3 BCH154 Bioenerget 3 1 0 4 4 3 BCH154 Immunol 3 1 0 4 4

4 BCH154 Practical I 0 0 3 3 6 4 BCH154 Practica 0 0 3 3 6

Ability Enhancement Compulsory Course Ability Enhancement Compulsory Course

5 CEN984 Communic 1 1 5 CEN984 Communi 1 1

6 BHS984 Behaviour 1 1 6 BHS984 Behaviou 1 1

Elective: Discipline Specific DSE Elective: Discipline Specific DSE

7 BCH154 DSE – 1 3 1 4 4 8 BCH154 DSE – 2 3 3 3

3rd semester 4th semester

S Subject Names of L T P C TC S Subject Names L T P C T

1 BCH154 Adavnced 2 1 0 3 3 1 BCH154 Microbiol 2 1 0 3 3

2 BCH154 Clinical 2 1 0 3 3 2 BCH154 Membran 2 1 0 3 3

3 BCH154 Practical 0 0 3 3 6 3 BCH154 Practica 0 0 3 3 6

Ability Enhancement Compulsory Course Ability Enhancement Compulsory Course

4 CEN984 Comm. 1 1 4 CEN984 Comm 1 1

Ability Enhancement Elective Course (AEEC)

6 BCH154 DSE – 3 3 1 4 4 5 BCH154 DSE - 6 3 1 4 4

7 BCH154 DSE – 4 3 1 4 4 6 BCH154 DSE - 7 3 1 4 4

8 BCH154 DSE – 5 3 1 4 4 7 BCH154 DSE - 8 3 1 4 4

Project Dissertation Project Dissertation

9 BCH154 Minor 5 10 9 BCH154 Major 9 18

DISCIPLINE SPECIFIC ELECTIVE

SEMESTER 1ST SEMESTER 2ND SEMESTER III SEMESTER VI

Bioanalytical technique Nutritional Environmental Science Biochemistryof

Microbial Biochemistry Biomaterials

Modules Course content Periods

Introduction to bioinformatics & computational biology; applications of bioinformatics;

1. Principles of physical chemistry- Puri,Sharma

Facilitating the achievement of Course Learning Outcomes

Module Course Learning Teaching and Assessment Tasks

I Students will be given basic Chalk and board Discussions, oral

PAPER II:CELL BIOLOGY AND PHYSIOLOGY

The course entails to educate the student about

Modules Topics / Course content Periods

Cell classification: Cell variability (size, shape, complexity,