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Labelling
Fixation
Dehydration
Clearing Tissue processing
Infiltration
Embedding
Factors affecting tissue procesing
Heat-45°C ideal
Agitation
Vacuum
viscosity
Concentration &
time interval of
specific steps
Nature of tissue
Nature of
examinations
needed
Labelling
Buffer & pH
Temperature
Concentration of fixative & ionic
composition
Duration of fixation & volume of
specimen
Additives
Formalin/formaldehyde (H-CHO)
Picric acid
Carnoy’s fluid
Post-mordanting
Dehydration
Removal of intra- & extra-cellular water so
that it may be replaced by embedding
media.
Hydrophilic polar groups bind water
Graded ethanol-70%,95%,absolute
alcohol(start with 50% for delicate tisue)
isopropyl alcohol, methanol, acetone,
butanol,ethylene glycol.
Universal solvents-dioxane.
Clearing
Tissue-tek molds
50%alcohol/ 1hr
formalin
70%ethanol 30min
95%ethanol 30min each
(2)