Professional Documents
Culture Documents
3. Complete the DNA sequence below such that the final sequence is identical to that of the
complementary strand. Your answer would be a seven-letter string composed of letters A, C, T,
and G only, e.g. TTCTCAG.
5′- C T T T A G A -3′
4. A DNA nucleotide pair has an average mass of approximately 660 daltons. Knowing the
number of nucleotides in the human genome, how many picograms of DNA are there in a diploid
human nucleus? Avogadro’s number is 6 × 1023. Write down the picogram amount without
decimals (round the number to the closest integer), e.g. 23 pg.
5. Which of the following features of DNA underlies its simple replication procedure?
A. The fact that it is composed of only four different types of bases
B. The antiparallel arrangement of the double helix
C. The complementary relationship in the double helix
D. The fact that there is a major groove and a minor groove in the double helix
6. Which of the following correlates the best with biological complexity in eukaryotes?
A. Number of genes per chromosome
B. Number of chromosomes
C. Number of genes
D. Genome size (number of nucleotide pairs)
7. Indicate true (T) and false (F) statements below about the human genome. Your answer
would be a six-letter string composed of letters T and F only, e.g. FTFFFT.
( ) Only about 1.5% of the human genome is highly conserved.
( ) Almost half of our genome is composed of repetitive sequences.
( ) Genes occupy almost a quarter of the genome.
( ) There are roughly as many pseudogenes in the human genome as functional genes.
( ) Transposable elements occupy almost 10% of our genome.
( ) On average, exons comprise 1.5% of our genes.
8. Chromosome 3 contains nearly 200 million nucleotide pairs of our genome. If this DNA
molecule could be laid end to end, how long would it be? The distance between neighboring base
pairs in DNA is typically around 0.34 nm.
A. About 7 mm
B. About 7 cm
C. About 70 cm
D. About 7 m
E. None of the above
9. For the Human Genome Project, cloning of large segments of our genome was first made
possible by the development of yeast artificial chromosomes, which are capable of propagating in
the yeast Saccharomyces cerevisiae just like any of the organism’s 16 natural chromosomes. In
addition to the cloned human DNA, these artificial vectors were made to contain three elements
that are necessary for them to function as a chromosome. What are these elements? Write down
the names of the elements in alphabetical order, and separate them with commas, e.g. gene,
histone, nucleosome.
10. Indicate whether each of the following descriptions better applies to a centromere (C), a
telomere (T), or an origin of replication (O). Your answer would be a seven-letter string
composed of letters C, T, and O only, e.g. TTTCCTO.
( ) It contains repeated sequences at the ends of the chromosomes.
( ) It is NOT generally longer in higher organisms compared to yeast.
( ) Each eukaryotic chromosome has many such sequences.
( ) There are normally two such sequences in each eukaryotic chromosomal DNA
molecule.
( ) There is normally one such sequence per eukaryotic chromosomal DNA molecule.
( ) It is where DNA duplication starts in S phase.
( ) It attaches the chromosome to the mitotic spindle via the kinetochore structure.
11. The eukaryotic chromosomes are organized inside the nucleus with a huge compaction
ratio of several-thousand-fold. What is responsible for such a tight packaging?
A. The various chromatin proteins that wrap and fold the DNA
B. The nuclear envelope which encapsulates the chromosomes
C. The nuclear matrix that provides a firm scaffold
D. All of the above
12. The two chromosomes in each of the 22 homologous pairs in our cells ...
A. have the exact same DNA sequence.
B. are derived from one of our parents.
C. show identical banding patterns after Giemsa staining.
D. usually bear different sets of genes.
E. All of the above.
13. Compared to the human genome, the genome of yeast typically has …
A. more repetitive DNA.
B. longer genes.
C. more introns.
D. longer chromosomes.
E. a higher fraction of coding DNA.
14. Indicate true (T) and false (F) statements below regarding histones. Your answer would
be a six-letter string composed of letters T and F only, e.g. TTFFFF.
( )
The histones are highly acidic proteins.
( The histone fold consists of three α helices.
)
( )
The core histones are much more conserved than the H1 histone.
( )
The N-terminal tails of the core histones undergo a variety of reversible post-
translational modifications.
( ) Every nucleosome core is made up of three polypeptide chains.
( ) The H1 histone is absent in the 30-nm fibers.
15. Indicate which feature (1 to 4) in the schematic drawing below of a chromatin fiber
corresponds to each of the following. Your answer would be a four-digit number composed of
digits 1 to 4 only, e.g. 2431.
2
4
3
1
16. In assembling a nucleosome, normally the …(1) histone dimers first combine to form a
tetramer, which then further combines with two … (2) histone dimers to form the octamer.
A. 1: H1–H3; 2: H2A–H2B
B. 1: H3–H4; 2: H2A–H2B
C. 1: H2A–H2B; 2: H1–H3
D. 1: H2A–H2B; 2: H3–H4
E. 1: H1–H2; 2: H3–H4
17. The chromatin remodeling complexes play an important role in chromatin regulation in
the nucleus. They …
A. can slide nucleosomes on DNA.
B. have ATPase activity.
C. interact with histone chaperones.
D. can remove or exchange core histone subunits.
E. All of the above.
18. Which of the following is true regarding heterochromatin in a typical mammalian cell?
A. About 1% of the nuclear genome is packaged in heterochromatin.
B. The DNA in heterochromatin contains all of the inactive genes in a cell.
C. Genes that are packaged in heterochromatin are permanently turned off.
D. The different types of heterochromatin share an especially high degree of compaction.
Another random document with
no related content on Scribd:
The place became, from about the middle of the eighteenth
century, a favourite resort of London anglers and swimmers, and
many London merchants and persons of good position were among
the subscribers. An annual payment of one guinea entitled
subscribers to the use of the baths, and to the diversion of “angling
and skating at proper seasons.” Occasional visitors paid two shillings
each time of bathing.
THE PLEASURE BATH, PEERLESS POOL, CITY
ROAD.
TERMS OF SUBSCRIPTION.
PLEASURE BATH
£. s. d.
Month 0 9 0
Two Months 0 16 0
Year 1 1 0
Single Bathe 0 1 0
with Towels
and Box
Ditto without 0 0 0
COLD BATH
£. s. d.
Month 0 10 0
Two Months 0 17 0
Year 1 10 0
Single Bathe 0 1 0
The City Road is the line from all parts of the West
End to the City. Omnibuses pass both ways nearly every
minute throughout the day.
1, Bath Buildings Entrance—2, Baldwyn Street
Entrance—3, Cold Bath—4, Pleasure Bath—5, Dressing
Boxes—6, Shrubberies.
BILL OF PEERLESS POOL. Circ. 1846.
About 1805 Mr. Joseph Watts (father of Thomas Watts, the well-
known Keeper of Printed Books at the British Museum) obtained a
lease of the place from St. Bartholomew’s Hospital at a rental of
£600 per annum, and eventually saw his way to a profit by building
on part of the ground. He drained the fish-pond which lay due east
and west, and built the present Baldwin Street on the site. The old-
fashioned house inhabited by Kemp, which stood in a garden and
orchard of apple and pear trees overlooking the west end of the fish-
pond, Watts pulled down, erecting Bath Buildings on the spot.[81]
The pleasure bath and the cold bath he, however, continued to open
to the public at a charge of one shilling, and Hone gives a pleasant
description of it as it was (still in Watts’s proprietorship) in 1826. “Its
size,” he says, “is the same as in Kemp’s time, and trees enough
remain to shade the visitor from the heat of the sun while on the
brink.” “On a summer evening it is amusing to survey the conduct of
the bathers; some boldly dive, others ‘timorous stand,’ and then
descend, step by step, ‘unwilling and slow’; choice swimmers attract
attention by divings and somersets, and the whole sheet of water
sometimes rings with merriment. Every fine Thursday and Saturday
afternoon in the summer, columns of blue-coat boys, more than
three score in each, headed by their respective beadles, arrive, and
some half strip themselves ere they reach their destination; the rapid
plunges they make into the Pool, and their hilarity in the bath, testify
their enjoyment of the tepid fluid.”
The Pool was still frequented in 1850,[82] but at a later time was
built over. Its name is kept locally in remembrance by Peerless
Street, the second main turning on the left of the City Road, just
beyond Old Street, in coming from the City. This street was formerly
called Peerless Row, and formed the northern boundary of the
ground laid out by Kemp.[83]
[Maitland’s Hist. of London, i. p. 84, ff.; Dodsley’s London,
“Peerless Pool”; Noorthouck’s London, p. 756, ff.; Trusler’s London
Adviser (1786), p. 124; Hone’s Every Day Book, i. p. 970, ff.;
Pennant’s London, p. 268; Wheatley’s London P. and P. iii. s.v.;
newspaper cuttings, &c., W. Coll.]
VIEWS.
1. Two woodcuts (pleasure bath and fish-pond) from drawings, circ.
1826, by John Cleghorn in Hone’s Every Day Book (cited above).
2. View of Peerless Pool Bath and Gardens in 1848; coloured
drawing by Read. Crace, Cat., p. 608, No. 9.
3. The Pleasure Bath, Peerless Pool. An advertisement bill with
woodcut of the bath, surrounded by trees and shrubberies, and a plan
of the vicinity (1846?), W. Coll.; cp. Crace, Cat., p. 608, No. 8.
THE SHEPHERD AND SHEPHERDESS, CITY ROAD
MARYLEBONE GROUP
MARYLEBONE GARDENS
VIEWS.
1. A view of Marybone Gardens and orchestra, J. Donnowell del.
1755; published by J. Tinney. Crace, Cat. p. 566, No. 74.
2. Modifications of 1, published by R. Sayer, 1755, and by Bowles
and Carver. Crace, Cat. p. 566, Nos. 75, 76. Also 1761, published by
J. Ryall [W. Coll.].
3. Views of Rose of Normandy. Crace, Cat. p. 566, Nos. 79–81; p.
567, No. 82.
THE QUEEN’S HEAD AND ARTICHOKE
VIEWS.
1. A water-colour drawing by Findlay, 1796. Crace, Cat. p. 569, No.
104; cp. an engraving of the inn in Walford, v. p. 258, and a small
sketch in Clinch’s Marylebone, p. 45 (dated 1796).
2. An engraving published in Gent. Mag. 1819, pt. 2, p. 401;
reproduced in Clinch’s Marylebone, facing p. 40.
THE JEW’S HARP HOUSE AND TEA GARDENS
VIEWS.
The Jew’s Harp public-house in Marylebone Park. A water-colour
drawing by Bigot, 1794. Crace, Cat. p. 569, No. 106; cp. a sketch in
Clinch’s Marylebone, p. 48.
THE YORKSHIRE STINGO
VIEWS.
1. “The Yorkshire Stingo in 1770,” a small sketch in Clinch’s
Marylebone, p. 46, showing the tavern and the entrance to the tea-
gardens.
2. View of the new County Court and the Baths and the Wash-
Houses, built upon the ground of the late tea-gardens, &c., of the
Yorkshire Stingo Tavern. A woodcut, 1849. Crace, Cat. p. 567, No. 89.
BAYSWATER TEA GARDENS
and in this garden he grew the plants for his wonderful Water
Dock Essence and Balm of Honey.
Hill died in 1775, and his garden was (some years before 1795)
turned into a place of amusement, known as the Bayswater Tea
Gardens, and much frequented by the denizens of Oxford Street and
neighbourhood.[124] Views of 1796 show the boxes and arbours, and
a family party, more plebeian than that in George Morland’s “Tea
Garden,” in full enjoyment of their tea. Waiters are bustling about
with huge kettles crying “’Ware kettle, scaldings!”
The Bayswater Tea Gardens are mentioned in the Picture of
London, 1823–1829, among those frequented by Londoners of the
middle classes. From about 1836 they appear to have been called
the Flora Tea Gardens, Bayswater.