Professional Documents
Culture Documents
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Submitted: 20-04-2023 Accepted: 30-04-2023
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ABSTRACT: bacteria. The Moringa leaves based toothpaste
In this study, Formulating an Moringa leaves based showed high inhibition against the selected
herbal toothpaste which product from the formation bacteria. This Moringa based toothpaste were
of biofilm in mouth mostly by the oral organism carried out with various phytochemical analysis
Streptococcus mutans. Dental caries are very like flavonoids, alkaloids, tannins, saponins,
common problem in human health and dental caries phenols, amino acids, proteins etc., And the
are mostly affected by bacteria and fungi Moringa leaves based herbal toothpaste carried out
microorganisms. The main aim of this study to the acceptability and long and shelf life.
overcome the forming biofilm in mouth which Key words: Biofilm formation-Streptococcus
leads to cause human infections, and sometimes it mutans-Moringa leaves- antibacterial -herbal
may cause periodontal diseases. Plaque contain a toothpaste.
variety of microorganisms. The pathogenicity of
certain microbial species such as Streptococcus I. INTRODUCTION
mutans, Staphylococcus epidermidis, Candida Dental caries (tooth decay) which leads to
albicans, Lactobacillus, Legionella pneumophilia, damage the tooth that happens when decay-causing
Pseudomonas aeruginosa, Escherichia coli. Here by bacteria in your mouth. That makes acids that
the Streptococcus mutans has the capable of attack the tooth’s surface. Due to the involvement
colonizing the oral cavity and also forms the major of high acids content in mouth which leads to the
biofilm formation. Biofilm formation has the small holes in tooth which expressed as cavity in
process of protein-bacterium interaction. By using mouth. If the tooth decay is not treated immediately
Moringa oliefera as a herbal plant to overcome it cause harmful infections, or it leads to diseases in
from the biofilm formation in mouth. Moringa oral care. By simple word the dental caries is the
oliefera is are used as traditional system of breakdown of teeth due to the microbial activity
medicine. This species is known as “The Miracle like bacteria and fungi. Dental caries are most
tree” because it is useful in the curing various common health problem in the world, is a chronic
diseases. Each and every part of Moringa plant like disease that destroys tooth tissue and that can
root, leaves, bark, stem, seed, flower are adversely affect chewing and aesthetic appearance
responsible for curing various diseases in human. (9). Nearly 60-70% of the child population is
Moringa leaves has good antibacterial activity affected by this dental caries. More or less the
which inhibit the growth of bacterial organisms. dental caries or tooth decay are effected by both
Moringa leaves has the both Anti-bacterial and gram positive and negative microorganisms. The
Anti-inflammatory activity. It mostly inhibits the main affecting agents of this dental caries are
Streptococcus species organisms. By formulating pathogenic microorganisms like Streptococcus
the antimicrobial toothpaste was evaluated by the mutans, Streptococcus mitis, Streptococcus
antimicrobial sensitivity test against the selected sanguis, Streptococcus salivarius and Streptococcus
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 1993
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
sobrinus are the microbial bacteria which causes products and fields with no exceptions (1). Herbal
tooth decay in mouth(2). The most common oral medicine have high demands due to their problem
and dental health diseases like tooth decay, arising with artificial medicine such as their cost
gingivitits, and periodontitis which causes and side effects to human health that is why the
frequently in humans in different stages of age herbal medicine has the huge demands. Utilization
groups(1). In this development of tooth decay or of the herbal based products which may be more
dental caries diseases, number of microorganisms, helpful and harmless for the human health care(4).
such as Streptococcus mutans and Lactobacillus are Moringa oliefera belongs to the family,
the two microbes which has the significant effects Moringaceae and order Brassicales (1). Moringa is
in causing dental caries in human, because they also known as drumstick or horse radish in English.
play a key role in fermenting the sugar molecules This Moringa plant has several medicinal and
like sucrose which results in producing the lactic nutritional values in each and every part of the
acids which involve in demineralization of cavity plant. And this Moringa is very healthy nutritive
and dental plaque. And some other microbes like vegetables(1). This Moringa plants is also known
Staphylococcus aureus, Streptococcus mitis, and as “The miracle tree”, because it has the ability to
Candida albicans have been also involved in dental cure the various diseases involved in mouth and
diseases. A majority of the involvement on the dental caries. Some of the research has began to
epidemiology of dental caries suggests that isolate the bioactive compound from the Moringa
Streptococcus mutans is the one of the major plant to known about their applications in various
pathogenic and carcinogenic bacteria in initial stage field(6). This Moringa leaves are usually used for
of formation of biofilm in mouth of dental food, traditional medicine and traditional ritual
caries(19). ingredients. It is noticed that Moringa leaves
This study mainly aims to reveals the contains more vitamin A than carrots, more
most pathogenic biofilm forming microorganism in calcium from milk, more iron from spinach, more
mouth which is inhibited by using the herbal based vitamin C from oranges and potassium from
toothpaste to overcome the common oral care banana[3,4]
problem arising in human generation. Biofilm Moringa oliefera demonstrates
formation are formed by many of the microbes like immeasurable potential in their view of the
Streptococcus mutans, Staphylococcus aureus, various biological activities which are reported
Lactobacillus, Klebsiella, Escherichia coli etc., In from its secondary compounds, such as alkaloids,
this Streptococcus mutants has the major flavonols, steroids, saponins, coumarins, quinones,
involvement in the process of forming biofilm resins, lectins among others(6). Based on the
formation in mouth. The additional properties of information and results acquired in other studies,
S.mutants has the capable of colonizing in oral this review aims to expose the potential of Moringa
cavity and able to survive in acidic environment leaves benefits which associates with remedy for
(13). Streptococcus mutants are responsible for biofilm formation in dental decay. Now this
bacteria adhesion and the formation of microbial Moringa leaves has the good side like rich in their
colonies on the host cell surface specially when it is vitamins, antioxidants, and minerals. Moringa
influences by human saliva. When this structure are leaves also contain the characteristics of fight
engaged in pathogenic interaction with host cells against inflammation and it protects the liver.
and they are responsible for formation of clustering Moringa leaves is good for detoxification. Moringa
of bacteria in the form of biofilm-like structure leaves has interaction with thyroid medicine,
(24). Most of the studies in article suggesting that medicine breakdown by the liver, diabetes and
Streptococcus mutans are the only organism are blood pressure. The involvement of Moringa leaves
isolate have a greater ability to form biofilm than in dental caries is, it can be used as an ingredients
the other Streptococcus species involved in the in toothpaste, mouthwash, root canal irrigation,
formation of biofilm, which colonize in the human wound healing after tooth extraction are can be
tooth decay or dental caries(21, 22). used to prevent the dental caries(8). Here the
Here the formation of biofilm can also be Moringa leaves has been used as main ingredient in
inhibited by the herbal plant which should have the the formulation of toothpaste against the formation
anti-bacterial and anti- inflammatory activity of biofilm. Moringa leaves can enhance the
against the dental caries. Herbal medicine is an rebuilding of enamel surface lesions and this
alternative formulation to chemically prepared Moringa gas the ability to deposits a new layer that
products in every aspects in pharmaceutical resembles the structure of healthy enamel. So this
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 1994
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
extraction of Moringa leaves against high and fungi. Dental caries are mainly caused due to
proportion of Streptococcus mutans forming no proper cleaning teeth well, frequent snacking
carcinogenic biofilm. This herbal leave has the and sipping sugary drink are main reason behind
antimicrobial activity against the pathogenic the cavities. Due to this reasons, the
bacteria present in tooth decay. If this benifical microorganisms are more helpful for the further
herbal leaves as an main ingredient used in the dental caries leads to the oral diseases[15]. Cavities
toothpaste to avoid the biofilm formation by are permanently damages the hard surface of the
bacteria, the toothpaste inhibit the formation of teeth which develops into tiny openings or small
cariogenic biofilm by Steptococcus mutans due to holes. Mostly the initial stage for the dental caries
the good antibacterial activity of Moringa leaves starts from the formation of biofilm in your mouth
and the combination of water-insoluble essential by pathogenic microbes.
oils such as peppermint, eucalyptus, clove, Dental caries is always localized in the
cinnamon are used as flavouring agents in destruction of susceptible dental tissues by the
toothpaste. This flavouring agents also called as products produced from bacterial fermentation of
“masking agents” or “bitter blockers”. dietary carbohydrates(32). The significance of
The essential leave extract has prepared demineralisation are found in hard dental tissues,
by using the phytochemicals and most of the but the diseases may cause by the initiation of the
volatile substances has taken as peppermint as a bacterial biofilm which covers the whole tooth
flavouring agents and smell. In this review, the surface in mouth(33). This dental caries is the
paper aimed to provide information on formulating multifactorial diseases that are initially starts within
the toothpaste against the formation of biofilm in the microbiological shifts with biofilm and then it
mouth by pathogenic bacteria by essential is affected by salivary flow and which are
extraction leaves against the selected preventing by hygiene behaviour like cleaning
microorganism(1). teeth(33).
Poor oral hygiene is the causes of biofilm
Dental caries accumulation and there will specific group of
Dental caries is a biofilm-induced disease, bacteria responsible for tooth decay. Due to this the
resulting from interactions of microorganisms, bacteria may causes like plaque-induced
host-related factors and diet which form into inflammatory periodontal diseases(34).
formation of biofilm on tooth surface(35).The
common oral caries are caused by the both bacteria
Decay into
dentine-
Several
decay
Dental
caries
Subclinic Visible
al decay- enamel
very decay-
early Early
stage stage
decay decay
Diagram 1: Diagram represents the stages of dental caries (32).
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 1995
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
Table 1: Table shows the different types of bacteria and fungi in oral caries(44).
Bacteria Fungi
Lactobacillus
Porphyromonas gingivalis
Common oral microbes include both the sites of disease which initiate by formation of
bacteria and fungi. Dental caries can be caused due biofilm, estimation of salivary levels of these
to either aerobic or anaerobic microbial bacteria. organisms may be useful for assessing caries risk in
Oral bacteria include Steptococcus mutans, the patients and monitoring their response to
Porphyromonas gingivalis, Staphylococcus aureus, preventive measures(45). Pathogenesis of dental
Candida albicans, and Lactobacillus. caries and evaluation of inhibitory concentration of
Streptococcus mutants is the main component in herbal plants can be detected against oral pathogen
oral microbiota in biofilm formation(28). (36)
Porphyromonas gingivalis is a non-glycolytic gram
negative anaerobic bacteria involved in periodontal Virulence of biofilm formation
diseases. Lactobacillus organisms is a bacterium Virulence is described as an ability of the
that can ferment the sugar molecules to produce microbes to infect the host and cause a disease.
lactic acid(31). Virulence factors are the essential molecules that
Dental caries is usually associated with assist the bacterium colonize the host the cellular
increased numbers of streptococcus mutans and level[16]. Biofilm helps bacteria to evade the host
lactobacillus are the most bacterial organisms at defence mechanisms and persist for a longer time
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 1996
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
and causes several oral-associated diseases (17). transforms into several different types of biofilm
Biofilm formation can be formed in three different formation in the first layer of oral plaque. Biofilm
stages like attachment, maturation and formation begins the maturation process by the
dispersion(46). Biofilm formation are formed by aggregation of bacterial growth(50).
most common oral cavity infectious disease is The formation of biofilm begins with the
caused and plays an important role in forming coating of tooth surface of the salivary(50). This
biofilm by the microorganisms on teeth and gum salivary components such as proteins, histamine,
surfaces in dental caries(47). The extracellular lysozyme, peroxidase, mucin are involved in mouth
polysaccharides(EPS) also affect the physical and salivary. The bacterial components such as
biochemical properties of the biofilm (48). The glycosyltransferase, fructosyltransferase and
primary sources of extracellular polysaccharides lipoteichoic acid (51,23). Formation of biofilm
are glycosyltransferase(GTF) and process, the bacterial surface adhesions is played
fructosyltransferase(FTF), are forms interaction by salivary agglutination(52). The initial stages of
with sucrose and starch(49). biofilm formation and adhesion by Streptococcus
Biofilm can be referred as the mutans may be stimulated by the salivary proteins
communities of microorganisms which attached to and salivary agglutination(53). The expression of
the surface of the mouth which is approached by virulence factors of biofilm formation in the oral
the bacteria and fungi(56). Oral biofilm formation cavity may be modulated in two ways such as by
are said to be mixed species of microbial the bacterial growth in environment and interaction
communities, and their outgrowth and overgrowth between them(54).
can be expressed as oral diseases(57). The Interaction between the oral cavity with
formation of biofilm is a multistep and more their colonizing microorganisms are the major
complicated process in oral cavity. Main factors affecting the development of biofilm (54).
interaction are begins with the salivary The virulence of the oral microbes ia not depends
agglutinations and loaded microbes in mouth on the environmental condition, but also it may
mostly by Streptococcus mutans(20). The cause due to the composition of bacterial flora(55).
interaction between the salivary and pathogenic High range of microbes involved in biofilm
oral microbes which may cause the formation of formation by the Streptococcus mutans (55). This
further composed of cells which starts beginning biofilm formation can be generally treated with
the bacterial colonization in mouth(50). The effective antibiotics like Chlorohexidine, gluconate,
microbial species like Actionmyces specie, penicillin, methicillin, ampicillin and
Strepococcus species, Lactobacillus species, erythromycin(58).
Candida species. Now these microbes get
•Aerobic •Anaerobic
bacteria bacteria
Mucosal Tongue
Surface Surface
Salivary Bacteria
Material Tooth
Surface enamel
Plaque plaque
•Fungal •Facultative
microbes bacteri
Diagram 2: Flow chart shows the microbes involved in types of dental caries(3).
The communities of oral bacteria are like major role in dental caries(28). Dental caries is
Streptococcus mutans, Staphylococcus aureus, dynamic, and active structure. The role of sugars as
Lactobacillus, Porphyromonas gingivalis, the risk factors in the initiation process of dental
Escherichiacoiland Candida albicans are plays a caries (59).
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 1997
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
The preventive measures should be taken called in several names. The leaves of Moringa are
for the most common dental disease occurred by usually used for food, traditional medicine and
the accumulation of bacterial growth in human traditional ritual ingredients[18]. One of the
mouth. Chemical agents are available in market treatment used by leaves is jaundice. This Moringa
areas, but by using herbal materials against the leaves contains more vitamin A, calcium, iron,
bacteria can be more benefit for oral health. Herbal vitamin C and more potassium(27). Moringa
materials should have their own anti-bacterial leaves and seeds has both anti-bacterial, anti-
activity and anti-inflammatory activity which inflammatory and anti-fungal activity against the
indicates oral health of human. Antibiotics like dental caries which also protect against
hexetidine, delmopinol, tricolsan, phenolic diabetes[12].
compounds which may inhibits the maturation and Moringa plant is high in polyphenols like
development of biofilm formation as well as effect flavonoid and phenolic acids are prominent(4).
of bacterial metabolism(60). Moringa is a traditional system of medicine which
is used to cure the diseases like malaria, typhoid
Moringa oliefera plant fever, parasitic diseases, arthritis, hypertension and
Moringa (Moringa oliefera) are used as diabetes. Moringa leaves which includes the
traditional and medicinal plant(4). Moringa plant is antioxidant, anti-inflammatory anti-microbial,
a natural-gift for several issues arises by anticancer, anti hypertensive and anti-proliferation
microorganisms[25].Moringa plant is also called as activities(61). Moringa demonstrates the
“the miracle tree” because each part of Moringa uncountable various biological activities which are
plant like plant from leaves, bark, seeds, fruits, root reported from secondary components like alkaloids,
are used by the human in their daily life(8). tannin, flavonoid, steroids, saponins, coumarins,
Moringa belongs to the family of Moringaceae . quinones, resins, lectins among others(6). In the
Moringa is fast growing soft wood tree which reach traditional system of medicine, the Moringa is
about 12m in height. This plant can grow in both mainly involves in the treatment of skin diseases,
dry and moist condition. Flower and fruits of the anemia, cholera, headaches, anti hypertensive,
Moringa oliefera originates twice in a year(4). This regulator of thyroid hormones, laxatives and as
herbal plant are known to horse radish, drumstick, antibiotics(6).
Moringa are the nicknames for Moringa oliefera
Antioxidant
Anti-microbial Anti-
inflammatory
Anti- Anti-cancer
hypertensive
Moringa leaves at the flowering stage can be used infections (4). Moringa plant in the community and
against microbial strains causing oral and wound research regarding he usefulness in the human oral
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 1998
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
cavity. Moringa leaves has the more active leaves has an anti-inflammatory effect on male
ingredients can be used as an antibacterial agent in white rats. Moringa leaf ethanol extract is anti-
oral cavity. Moringa oliefera has more potential inflammatory.
anti-inflammatory in human oral cavity (8). This Moringa leaves has helps in
Moringa plant can be consumed as a vegetable and suppressing the growth of oral bacteria like
drink, fruits and seeds. Moringa leaves has used Staphylococcus aureus and E.coil. Moringa leaves
for the treatment and management of different has antimicrobial properties on the leaves and
diseases in traditional medicine. Moringa leaves seeds. This herbal Moringa leaves acts as an
has three important character in oral caries. antibacterial agent in oral caries or dental caries,
namely bacteria like Porphyromonas gingivalis,
Role of Moringa leaf in oral caries Prevotella intermedia, Staphylococcus aureus and
The main role of Moringa leaf is anti- Streptococcus mutans. Hence the Moringa leaves
bacterial and anti-inflammatory activities which extract has an antimicrobial activity against the
play important role in oral caries. Moringa leaves is pathogenic bacteria and biofilm formation. Here
an alternative herbal medicine for use an anticancer proving the properties of Moringa leaves against
agent. Moringa leaves are known to cure the wound the biofilm formation by the oral carcinogenic
healing infections. Moringa leaves extraction are bacteria(8).
used as herbal medicine in the oral product for the
oral health benefits.The ethanol extract of Moringa
Bioactive
components of
1.Anti- bacterial
activity in moringa 2.Anti-inflammatory Moringa plant:
leaves which inhibits properties to reduce
bacterial growth. inflammation in oral 3.Anti-microbial
properties involved
cavity. in inhibition of
growth of oral
microbes.
Anti-bacterial activity of Moringa plants The main reason for the Moringa leaves is
Moringa leaves has rich in antibacterial used for the prevention of inhibiting the biofilm
activity against the oral pathogenic microbes. The formation by bacteria. The biofilm in the oral
use of medicinal plant as an natural ingredient to cavity is structurally and functionally related with
cure the diseases related to human oral cavity. bacteria. The proposition of Streptococcus mutans
Plants are used as medicine for human health growth is increased due to environmental changes,
benefits and also they contain beneficals chemicals. the oral biofilm which becomes carcinogenic
These activities prevent against the pathogenic biofilm when it get overgrowth.
bacteria and fungi(8). The antimicrobial in leaves and antioxidant
The antibacterial properties found in this in flower and bark properties of the Moringa plant
herbal Moringa leaves which makes this plants is used to formulated for the dental caries to
widely used in dentistry. Moringa plants from control the oral pathogens[26]. This Moringa based
leaves, seeds, flowers, bark to root have their herbal product may be toothpaste or mouthwash
tremendous benefits for human. The use of herbal which inhibit the growth of bacteria which is
based Moringa plant used in dentistry acts as an responsible for the formation of biofilm. Root canal
antibacterial agent in oral bacteria(8). treatment requires ingredients like antibacterial
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 1999
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
properties which gain successful results in the properties which is normal protective response
treatment. The medicinal plant of Moringa plants mechanism against tissue caused by microbial
which have their antimicrobial properties and agents. Moringa leaves has anti-inflammatory
active components has valuable purpose I curing effect due to the activity of secondary
disease or infection of the oral cavity(8). metabolitescontains in ethanol extract of Moringa
leaves, namely flavonoid. Moringa leaves can
Anti-inflammatory activity of Moringa plant inhibit the activity of inflammation by the
Health factors is one of major important enzymatic activity. Moringa leaves itself has its
role which plays in human daily life. The health of own anti-inflammatory range upto140 mg/ kgBW.
the oral cavity often neglected. Oral health is also Moringa leaves extraction has the good action
an important factor which plays major role in against as anti-inflammatory activity and useful in
determining the quality of human life and this oral health care in oral cavity or tooth decay.
cavity infection is more common diseases in human
life, but its causes major defects in healthy life(8). Phytochemical analysis
To avoid the oral issues, herbal based The yield obtained by Moringa based toothpaste
things can be used as an medicine. Moringa leaves was 100gm. The result is concluded by the
has distinct properties which acts as an medicine confirmatory phytochemical analysis of Moringa
for health. Moringa plant has the anti-inflammatory leaves extraction was presented in table1.
Table 3 :This table shows the physio chemical analysis of for formulated toothpaste and control
toothpaste
S.No Parameters Herbal toothpaste Control toothpaste
1. Moisture 49% 54%
2 Color Green Blue
3 Homogenous Homogenous
Homogenicity
4 Texture Smooth Smooth
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 2000
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
5 pH 7.70 7.40
6 Clogging Absent Absent
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 2001
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 2002
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 2003
International Journal of Pharmaceutical Research and Applications
Volume 8, Issue 2 Mar-Apr 2023, pp: 1993-2004 www.ijprajournal.com ISSN: 2249-7781
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[56]. Hamza AA[2010].Ameliorative effects of activity of miswak extract and different
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DOI: 10.35629/7781-080219932004 | Impact Factor value 7.429 | ISO 9001: 2008 Certified Journal Page 2004