Lab.5 – PR. HUMAN PHYSIOLOGY MR.

SARWAR MOHAMMED

WHITE BLOOD CELLS COUNTING

 White blood cells (WBCs) count: is a blood test to measure the number of (WBCs) in
the blood sample. It is a part of a complete blood count (CBC), are also called leukocytes.

 White blood cells abnormalities:

- When the number of WBCs

increased is called
Leukocytosis
- When the number of WBCs
decreased is called
Leukopenia

LEUKOCYTOSIS
 A condition characterized by
elevating the number of WBCs in
the blood, which is usually due to:
- Infection such as appendicitis,
ulcers and urinary tract infection
- Certain types of cancer, such as
leukemia
- Pregnancy
- Injury or surgery
- Bone marrow or immune disorder
LEUKOPENIA
 A condition characterized by reducing the
number of WBCs in the blood, which is usually
due to:
- Viral disease such as hepatitis, and HIV
- Some bacterial infections such as typhoid fever
- Rheumatoid arthritis
- Radio therapy and chemotherapy
- Bone marrow diseases, not enough making
WBCs
- Tuberculosis

 Principle: the number of WBCs is very high, so that they diluted 20 times with the certain
diluting fluid, which is called Turk's fluid. Then start counting the WBCs.
Lab.5 – PR. HUMAN PHYSIOLOGY MR. SARWAR MOHAMMED

 Requirements
1. Materials to sterilize & finger prick
2. Hemocytometer (Neubauer) slide
with coverslip
3. WBCs pipette
4. Microscope
5. Turk's fluid (WBCs diluting fluid)
6. Blood sample

 The normal range Leukocyte are: 4000– 11000 cell / mm³ of blood

 Function of Turk's fluid

1. Destroy the RBC (by acid)
2. Dilute the blood
3. Staining the nucleus of WBCs to increase visibility

 Procedure
1. Clean the finger & get skin puncture.
2. Draw the finger blood and full the white blood cell pipette
till the mark 0.5 then draw Turk's fluid till the mark 11.
3. Mix the blood with the fluid.
4. Place the cover slip on the chamber slide.
5. Discard the first few drops and transfer little amount of the mixture on the chamber.
6. Leave the hemocytometer for a few minutes so the cells are settled down.
7. Start cell counting (4 large squares of the corners on the chamber), under power 10X.
8. Calculate the total number as follows:
Total WBC Count = N x 50 = ?? cell / mm³ of blood
Lab.5 – PR. HUMAN PHYSIOLOGY MR. SARWAR MOHAMMED
Lab.5 – PR. HUMAN PHYSIOLOGY MR. SARWAR MOHAMMED