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Objectives of the Study

The objective of the study was to determine the prevalence of Porcine Epidemic Diarrhea

(PED) in diarrheic pigs from selected swine farms in the province of Rizal, Philippines:

Specifically, this study aimed to:

1. detect the occurrence of Porcine Epidemic Diarrhea (PED) virus in pigs from commercial

and backyard swine farms in Rizal using ANDALI ® Porcine Epidemic Diarrhea Reverse

Transcription- Loop mediated Isothermal Amplification (RT-LAMP) test kit;

2. identify the probable risk factors associated with Porcine Epidemic diarrhea using a

structured questionnaire;

3. correlate the risk factors with the prevalence of Porcine Epidemic Diarrhea (PED) and;

4. develop a spot map of the distribution of Porcine Epidemic Diarrhea (PED) in the

province of Rizal.

Scope and Limitation of the Study

The study determined the prevalence of Porcine Epidemic Diarrhea (PED) among the

diarrheic swine herds in the province of Rizal, Philippines using ANDALI ® Porcine Epidemic

Diarrhea Reverse Transcription- Loop mediated Isothermal Amplification (RT-LAMP) test kit

only. All ages of diarrheic pigs inside the pen that exhibit clinical sign of diarrhea not more than

twenty-one (21) days before the visit was used in the study. This also identified the probable risk

factors associated with the disease using a structured questionnaire and correlated it with the

prevalence of PED. All swine farm with 1-20 sow level and/or below 99 young hogs were

considered as backyard farms and all swine farms that has higher population than the latter is
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considered as commercial farms as per the Department of Agriculture (2012) standards. Only

those that gave permission for inclusion of their farm were sampled in the study. Moreover, a

spot map was developed to determine geographic distribution of Porcine Epidemic Diarrhea in

the province. Only fecal samples were collected and this study did not measure the antibody

level against PED of the animals sampled.

Time and Place of the Study

Fecal samples were collected from commercial and backyard swine farms in the province

of Rizal and processed at the Department of Immunohistopatholgy and Microbiology of the

College of Veterinary Medicine and Biomedical Sciences June 2018 under the supervision of

Dra. Ma. Cynthia Dela Cruz.