Take a photo of your prepared sample slide, indicating the location of your five cells:

At 1x magnification (without the microscope)

At 40x magnification
At 100x magnification right most middle top/right
size masurements
Cell 1 SW 2 SE 3 NE 4 NW 5 S of NW
width (epd) 9 15 7 17 8
width (um) 90.909 151.515 70.707 171.717 80.808
r.o.s before adding cytochalasin
distance measured 202.02 303.03 303.03 808.08 303.03
time measured rep 1 6 14 11 18 9
time measured rep 2 6 20 11 18 7
time measured rep 3 5 15 10 29 7
time measured rep 4 6 15 11 23 10
time measured rep 5 6 14 10 18 10
rate of streaming rep 1 33.67 21.645 27.54818 44.89333 33.67
rate of streaming rep 2 33.67 15.1515 27.54818 44.89333 43.29
rate of streaming rep 3 40.404 20.202 30.303 27.86483 43.29
rate of streaming rep 4 33.67 20.202 27.54818 35.13391 30.303
rate of streaming rep 5 33.67 21.645 30.303 44.89333 30.303
adding cytochalsin
Which cytochalasin did you add? C
What was its concentration? 200 μM
What was it dissolved in? DMSO (Dimethyl sulphoxide)
How much did you add? 40 microL
r.o.s 10min after adding cytochalasin
distance measured 232.323 353.535 191.919 595.959 353.535
time measured 5 10 10 20 10
rate of streaming 46.4646 35.3535 19.1919 29.79795 35.3535
recovery at 5 min
distance measured
time measured
rate of streaming
recovery at 10 min
distance measured
time measured
rate of streaming
recovery at 20 min
distance measured
time measured
rate of streaming