Course Duration : 12 weeks

Course Start Date:

Course End Date:
Final Exam Date:
 About the Course
This course is designed to offer a holistic grasp of techniques in molecular diagnostics
implemented in healthcare sectors and research. Upon completion, participants will
gain a profound understanding of basic and advanced tools of gene expression analysis
and proteomics as well as their diverse application in clinical diagnostic fields.

COURSE CONTENT

Essential Fundamentals including DNA Replication and Repair, RNA

Week 1 Transcription and Processing, Protein Synthesis from mRNA-Translation, DNA &
RNA isolation Methods, Southern blotting techniques.
Tools of Molecular diagnostics and Gene expression Analysis (I)
Week 2
Including PCR and Real Time PCR and different variations of PCR
Tools of Molecular diagnostics and Gene expression Analysis (II)
Week 3
Recombinant DNA Technology & Genome Editing Tools (CRISPR)
Tools of Molecular diagnostics and Gene expression Analysis (III)
Week 4 DNA Microarray, FISH, Epigenetics & DNA methylation analysis, DNA protein
interaction techniques
Tools of Molecular diagnostics and Gene expression Analysis (IV)
Week 5
Traditional and next generation sequencing methods
Proteomics : Basic techniques for Molecular diagnosis
Week 6 LC/HPLC, Mass Spectrometry, Differential In Gel Electrophoresis, Protein
Microarray etc.
Proteomics : Advanced Techniques for Clinical Applications
Label-free and isotope labelling methods, Shotgun proteomics, Data independent
Week 7
acquisition (DIA), Single cell proteomics and spatial profiling, protein-protein
interaction, bioinformatics tools.
Molecular Diagnostics in Infectious Diseases
Week 8
Syndromic Panels and Multiplex Assay, RNA sequencing, Tiling array etc.
Molecular Diagnostics in Cancer Management
Week 9 Cancer markers, Liquid biopsies & circulating Tumour DNA (ctDNA) analysis,
Digital PCR & PCR Based mutation analysis
Molecular Diagnostics in Genetic and Inherited Disorders (Genetic testing and
Week 10
inherited diseases, Non-Invasive Prenatal testing and reproductive genetics)

Molecular Diagnostics in Medicine (RNA interference and detection methods,

Week 11
Luminex multiplex assays, Metabolomics in laboratory diagnosis)

Molecular Diagnostics : Quality control, Ethical Concerns, Informatics tools and

Week 12
Futuristic Trends, Integration of Multi-omics Data.