Homework 7

Name: _____________
Student ID: _____________

I. Perform MSA (multiple sequence analysis) to compare the results by using PSI blast and T-
Coffee to do the sequence alignment of the 6 coronaviruses. Show your results.
II. Check the researching results of using 2 different methods and then point out the significant
differences between them.

- Get the sequences from Protein Data Bank

- Chose the sequence of each one (you decided, and posted the one you chose with your
assignment results together)
- Get the sequences from “Exp. Method: X-RAY DIFFRACTION”

1. SARS Coronavirus Main Proteinase (3CLpro)

2. HUMAN CORONAVIRUS 229E
3. Porcine transmissible gastroenteritis virus (TGEV)

T-coffee http://www.ebi.ac.uk/Tools/msa/