Disinfection In

Animal Houses
 Cleaning and Disinfection (C&D) programs

Disinfection: a process that Disinfectant: (practical definition) :

eliminates many or all pathogenic Achieving at least 5 log reductions
microorganisms, except bacterial in microbes number i.e.; (99.999%
spores, on inanimate objects (CDC ) from the original number will be
Definition). removed after treatment
 How cleaning affects the disinfection process
quality ?
Cleaning is the removal
of soil (e.g., organic and Organic mater will
inorganic material) from 1. Neutralize / Deactivate
objects and surfaces and Organic matter =
Disinfectants, Microbes WHILE
normally is accomplished
mechanically using High 2. Shelter less organic
pressure water or microorganisms and matter = less
chemically using 3. Create resistant microbes
chemical detergents or microbial strains
enzymatic detergent.
DO NOT DISINFECT DIRTY AREA
When formulating disinfection program, Take in
your consideration :

M.O

Factors
affecting Soling
efficacy of
disinfectants
type

Soiling
degree
 M.O
Micro-organism Suitable Disinfectants Concentration
Spores Peracetic acid 5% 5%
Glutaraldehyde 50% (Activated) 2%
Accelerated Hydrogen Peroxide (AHP) 50% 4.5 %
Sodium Hypochlorite 6000 ppm
Mycobacterium Iodophor 3% titratable iodine 5%
Synthetic phenols ( phenyl phenol ) 3-5%
Foot and mouth Disease Sodium Hydroxide 50 % 4%
Sodium carbonate 8%
Citric acid 99% 3%
KMPS (VIRKON S) 2%
Coccidia Ammonium Hydroxide 7-10 %
Synthetic phenol ( Chlore Cresol ) 3%-5%
 Soiling type

Soil

Organic ( fat – CHO- Protein ) In organic ( Salts )

Alkaline Detergent Acidic Detergents

 Soiling Degree

No previous
Cement floor vs
infection vs
muddy floor
outbreak
 How to Apply ? (Application methods)
Cleaning : Pressure washer ( sprayer)
90 bar
Don’t use High Pressure Cleaning in any house infected with epidemic Respiratory disease
 How to Apply ?
Cleaning : Foam application
Low pressure cleaning – Visible – High contact time – less water and detergents consumption
Favorable incase of respiratory epidemics
 How to Apply ?
Disinfection : Low Pressure Spray (35 bar)
Disinfection : Foam Spray
 How to Apply ?
Aerial Disinfection

Knapsack sprayer ULV Cold sprayer Thermal fogger

50 -100 Micron 20 -50 micron 5 micron (don’t use in animal presence)
 Calculation ( Application rate)

Disinfection (Surface) : Every 1 m2

Cleaning : Every 1 m2 needs (0.5 l
water and detergent) to be thoroughly
immersed and cleaned ( incase of
spraying ) and (0.3 l water and
detergent ) incase of foaming
needs (0.3 l water and disinfectant ) to
be thoroughly immersed and
disinfected incase of no specific
pathogenic problem and (0.4 l to 0.5 l
water and disinfectant ) incase of
outbreaks
 Aerial Disinfection
Empty House
Heating Paraformaldehyde Blocks 91%
Formalin (40 %) and Potassium permanganate
Glutaraldehyde and QACS
Synthetic phenols
KMPS (VIRKON S)
Dose
5 grams per cubic meter of space.
Adding 35 mL of formalin (40 percent formaldehyde)
to 17.5 g potassium permanganate per cubic meter of
space.
1 Liter + 4 liters water / 1000 cubic meter air
Thermal fogging.
1 Liter + 4 liters water / 1000 cubic meter air
Thermal fogging
1l / 40 m2 ( Thermal fogging)
 How to Disinfect A cattle House infected with FMDV
Empty House – Cement Floor
• Remove all old animal bedding , feed, and water from the house / yard.
• Strat with the dry cleaning phase.
• Spray the house with Alkaline detergent solution at the rate of 0.5 l/m2. (2%) Or 1kg sodium
hydroxide /4 liter water
• Spray the house again with FMD approved disinfectant at the rate of 0.4l/m2. (NaoH,
Na.Carbonate, citric acid, KMPS)
• The removed mud, and bedding should be sprayed with Sodium Hydroxide solution until Ph 11 is
For 5 days.
• Feed and water troughs must also included in the disinfection phase.
• In closed houses aerial disinfection must be applied.
 How to Disinfect A cattle House infected with
FMDV Occupied House – MUD Floor
• When the Animals can not be moved from the house the suspected animals should be quarantined in a
separate house against the wind direction.
• Soil should be sprayed with Approved FMD disinfectant which must be mixed with alkaline detergent at
the rate of 100 ml to 1 liter disinfectant.
• Soil should be sprayed with previous mix at the rate of l / m2 .
• Remove the first 10 cm from the mud
• The removed mud, and bedding should be sprayed with Sodium Hydroxide solution until Ph 11 is For 5
days.
• Water must be sanitized with appropriate sanitizer.
• Air should be disinfected via fogging every 6 hrs.
• The yard surroundings should be also disinfected.
 How to Disinfect A cattle House infected with
Tuberculosis Occupied House – MUD Floor
• Soil should be sprayed with 5 % (iodophor – 3% titratable iodine)
• Soil should be sprayed with previous mix at the rate of l / m2 .
• Remove the first 10 cm from the mud
• The next 10 cm from the mud should be sprayed with synthetic phenol formula (5%)
• Remove the second 10 cm from the mud
• Don’t remove the next 10 cm (VIPPPPPP)
• The removed mud, and bedding should be sprayed with Sodium Hydroxide solution until Ph 11 is reached and
hygienically buried
• Water must be sanitized with appropriate sanitizer.
• The yard surroundings should be also disinfected.
Thank you