xpertmont: 6
Win — To taolate DNA from avatlable plank mobetal
Treory. / Putnciple — Recombinant-pN/v technology has allowed,
breeders. to fntscodiice pret DNA In othe ong anima
including bactest a, vend »plant- and, antmal,.Such ovganisms ax.
Genelealy modified: organlams (GMOs). Thus oobDNA dechnoleg
thvolve’, fzobadfon of DNA from vartely of Sources and,
formation +f news combination of DNA.
. Keg uixements _~ Plant makesual C banana) » mordax and.
peatle , beakese , teat tubes , diqutd, debesogent, aodium
chlostde, ethanol and dratilled, water.
Procedune =.
A Take be fect bananw
a Mahe wath sf benens and pestle and transfer tr.
a beakes .
2 Add foo mL dfsttlled, wate and algo add Nach,
WNow, add 4 teaspoon of Nigutd detexsgent-
5 Keep the mixtuxe ak xeat poe 5 minukes’ and pitte the
mintuxe tn test tube. .
6 Powe chilled ethanol bom aides of the test tube to make
distinc bayer. on the fop . This will allow. Dua to preetpitale o
t The predpttake tan be apooled, with the help of glass rod.Remarks,
Experiment No. ___
Lsotaton of pwat in teat lube (8
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3 “— Spo!)
— Dia thoeade
1 Atcobol
{=test Hibe
a
Teacher's signatureObacwation S—
The addfhion ok
prectpitake, The DNA Appears as white psectpttale ef
Bory fine thsieads on the glass Spool.
ethanol tm the solutfon causeg DNA to
Precautfons i
ag txesh plant- makouial gh
ould, be used, for exbraction of DNA.
% wll the qlasswares used must be thoscoughly cleaned ahd,
dsuted .
3+ The chenvoal, and, enzymes used, fos the expest ment should
be ef standased, quality.
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