SM BioIIAdvance EN 1013

SERVICE MANUAL
Microbiological Safety Cabinet
Bio II Advance
SM-BioIIAdvance-EN-1013 1 / 37
INDEX
0. COMPONENTS DESCRIPTION ________________________________________ 4

1. ELEMENTS LOCATION ______________________________________________ 5
2. REPLACEMENT OF ELECTRICAL ELEMENTS ______________________________ 6
2.1. Fuse of the power socket _______________________________________ 6
2.2. CPU card ____________________________________________________ 7
2.2.1. Test and replacement of the CPU fuses ________________________ 8
2.3. Control panel (Display) _________________________________________ 9
2.4. Power source ________________________________________________ 10
2.5. Replacement of the fluorescent tubes _____________________________ 11
2.6. Replacement of the UV light _____________________________________ 12
2.7. Replacement of the micro-switches _______________________________ 13
2.8. Replacement of the speed probe _________________________________ 16
3. REPLACEMENT OF MECHANICAL ELEMENTS _____________________________ 19
3.1. Replacement of the Extraction Filter _______________________________ 20
3.2. Replacement of the Impulsion Filter _______________________________ 21
3.3. Replacement of the frontal glass _________________________________ 22
3.4. Replacement of the springs of the glass ____________________________ 23
4. FUNCTIONAL TEST ________________________________________________ 24
5. OPERATION AND CONTROL OF THE CABINET ___________________________ 25
5.1. Start up of the cabinet _________________________________________ 25
5.2. Alarms _____________________________________________________ 26
5.3. User screens to be used in maintenance operations ___________________ 27
5.4. Control functions with Technical Service Password ____________________ 29
5.5. Calibration of the cabinet _______________________________________ 34
6. RECOMMENDED INSTALLATION TEST _________________________________ 36
6.1. Downflow Filters Integrity Test ___________________________________ 36
6.2. Exhaust Filters Integrity Test ____________________________________ 36
6.3. Downflow Air Velocity Test ______________________________________ 36
6.4. Set Points Alarm Downflow Air Velocity Test ________________________ 36
6.5. Inflow Air Velocity Test _________________________________________ 36
6.6. Set Point Alarm Inflow Air Velocity Test ____________________________ 36
6.7. Smoke Test __________________________________________________ 36
6.8. Lighting Test _________________________________________________ 36
6.9. Acoustic Level Test ____________________________________________ 36
7. ELECTRICAL DIAGRAMS ____________________________________________ 36
8. COMPONENTS ____________________________________________________ 36
8.1. Optional Components __________________________________________ 36
9. CLEANING _______________________________________________________ 37
9.1. Superficial Cleaning ___________________________________________ 37
9.2. Front Glass Disinfection and Cleaning ______________________________ 37
9.3. Formaldehyde Disinfection ______________________________________ 37
10. CERTIFICATES ___________________________________________________ 37
0. COMPONENTS DESCRIPTION
8 8
2
2
1 1
5 1
5
3
3
4
4
6
6
1. Centrifugal Blower
2. Exhaust flexible tube
3. Laminar flow chamber or plenum
4. Laminar flow HEPA filter
5. Exhaust HEPA filter
6. Working area
7. Outside air inlet and protection barrier
8. Exhaust outlet
1. ELEMENTS LOCATION
1 Exhaust outlet. 6 Checking valve fans pressure.
2 Power supply. 7 Gas and vacuum connections

(OPTIONAL).
3 Display and control panel.
4 CPU card.
5 Checking valve for D.O.P. control

(Differential pressure).
2. REPLACEMENT OF ELECTRICAL ELEMENTS
WARNING
Before any service task ensure to perform a proper decontamination of the cabinet when
applicable as shown in user manual Annex 5.3.
After finishing any service task, it must be ensured that the cabinet is working properly,
according the checklist shown in chapter 4.
WARNING
Before performing the following service tasks, unplug the power plug of the cabinet in order
to avoid any risk of electrical shock.
It must be ensured the right fixing of the frontal cover by means of the two roods that are
supplied for such purpose.
REMARK
When it is required for the specific service task, the frontal cover must be raised or put back
to the closed position only when the glass is in the closed position. The contacts of the
frontal cover must be pushed back manually.
2.1. FUSE OF THE POWER SOCKET

The main power socket has inside a protection fuse.
In order to replace this fuse it is necessary to open the cover where it is located, take the
fuse out and replace it by a new on that fulfils the following features:
T10A 250V 5x20mm.
2.2. CPU CARD
For the replacement of the CPU card, these steps should be followed:
a) Unplug the cord plug of the cabinet.

b) Remove the two M5 screws that hold the frontal cover and lift it up, fixing it with the two
rods provided for that objective. (Fig.1)
c) Disconnect all CPU card connectors (Fig.2)
d) Unscrew the M4 central screw and its washer (Fig.3)
e) Take out carefully the CPU card from its 4 PEM fixing points (Fig.4)
For the installation of the new CPU card, all steps should be carried out in reverse order,
reminding to connect the 4 groundings (3 + the central one) with its washers (Fig. 5 & 6),
and screwing the two M5 screws that fix the frontal cover. By replacing the chip (Fig. 7) of
the new card by the old one the calibration data are maintained. Otherwise it is necessary to
re-calibrate the cabinet.
Fig. 1 Fig. 2
Fig. 3 Fig. 4 Fig. 5
Fig. 6 Fig. 7
2.2.1. TEST AND REPLACEMENT OF THE CPU FUSES
The CPU card includes 3 fused for the lighting, sockets of the chamber and the fans.
In order to replace the fuses it is required to turn 180º the cover of the specific fuse holder,
take the fuse out and replace it by a new one that fulfills the following features:
F1
F2
F3
F1 – Lighting Fuse 4A 250V
F2 – Socket Fuse 4A 25 V
F3 – Fan fuse 10A 250V
2.3. CONTROL PANEL (DISPLAY)
For the replacement of the Control panel (display), these steps should be followed:

rods provided for that objective (Fig.1)
c) Disconnect the connector of the display card (Fig.2)
d) Unscrew the six M3 screws and its washers (Fig.3)
e) Take out carefully the display card.
For the installation of the new display, all steps should be carried out in reverse order,
reminding to screw the two M5 screws that fix the frontal cover.
Fig. 1 Fig. 2
Fig. 3
2.4. POWER SOURCE
For the replacement of the power source, these steps should be followed:

rods provided for that objective. At that point you can see the power source (Fig.1 & 2)
c) Disconnect all connectors of the power source (Fig.3)
d) Remove the two M4 screws and the two M4 nuts that fix the electronics support (Fig.4)
e) Remove the three M3 screws that fix, by the lateral, the power source (Fig.5)
f) Take out carefully the power source.
For the installation of the new power source, all steps should be carried out in reverse order,
reminding to screw the two M5 screws that fix the frontal cover.
Fig. 1 Fig. 2
Fig. 3 Fig. 4
Fig. 5
2.5. REPLACEMENT OF THE FLUORESCENT TUBES
For the replacement of the fluorescent tubes, these steps should be followed:

b) Take out the two M5 screws of the frontal cover of the lighting fitting (Fig.1).
c) Lift the lighting fitting, replace the old tubes by the new ones (Fig.2).
d) Screw again the two M5 screws of the frontal cover of the lighting fitting (Fig.1).
Fig.1
Fig.2
2.6. REPLACEMENT OF THE UV LIGHT
For the replacement of the UV light, these steps should be followed:

b) Detach the frontal-glass from the cabinet (by pulling from the lateral handles of the
metal support), and move the glass to the limit upper position.
In case the UV light design is assembled with clamps,
c) Take out the UV light from the clips that are fixing it. (Fig.1).
d) Disconnect the lamp-holders of the tube (Fig.1).
e) Assembly the new tube in the lamp-holder and fix it again carefully with the clips,
avoiding to pull the lateral cablings of the tube.
Fig.1
In case the UV light is assembled with lamp holders,
c) Take out the UV light from the lamp holders by rotating and pulling down the tube.
(Fig.2).
d) Assemble the new tube in the lamp-holders, by fully pulling up the tube on the slot of
the lamp-holders, and then rotating it.
Fig.2
2.7. REPLACEMENT OF THE MICRO-SWITCHES
For the replacement of the micro-switches and/or the glass working position actuator, these
steps should be followed:

c) For the replacement of one of the micros, take out the connectors and the two M3 nuts of
each micro (Fig.2)
d) Fort he replacement of the actuator sheet, take out the four M3 that fix the micros and
the actuator sheet (Fig.2)
e) For the installation of the new micros or actuator sheet, all steps should be followed in
reverse order, adjusting the actuator position with the nuts M4 (Fig.3) and checking that the
4 plastic spacing parts are below the micros (Fig. 4)
f) To check that they are well adjusted, put the cabinet and the glass in working position,
checking that the actuator sheet pushes both micros.
Fig. 1 Fig. 2
Fig. 3 Fig. 4
For replacing the glass closed position micro-switches, these steps should be followed:

b) Detach the frontal mechanism from the cabinet. That way it will be possible to access the
micro-switches (Fig.1) that are located at the right side of the cabinet.
c) Take out the two M3 nuts of the micro to be replaced (Fig.2)
d) Loose the opposite end of the cabling, that is screwed to the inferior part of the right
contact between the frontal cover and the frontal mechanism (Fig.3)
e) For the installation of the new micros or actuator, all steps should be carried out in
reverse order, and welding previously the micro to the cabling (Fig.2 & 4) and adjusting the
position in order the micros actuate correctly with the actuator (Fig. 1 & 5).
Fig. 1 Fig. 2
Fig. 3 Fig. 4
Fig. 5
For replacing the contacts between the frontal cover and the frontal mechanism, these steps
should be followed:

Upper part of the contacts:
c) The contacts are screwed to the frontal cover with two nuts, that should be unscrewed, as
well as the terminals of the cabling that get to the contacts (Fig.2 & 3)
Lower part of the contacts:
d) Detach the frontal mechanism from the cabinet. The contacts can then be accessed
(Fig.4) at the right side of the cabinet.
e) Release the opposite end of the cabling, that is screwed to the lower part of the contacts
between the frontal cover and the frontal mechanism, and remove the two nuts (Fig.5)
For the installation of the new contacts, all steps should be carried out in reverse order,
adjusting and checking the correct alignment between both upper and lower parts of the
contacts (Fig.6)
Fig. 1 Fig. 2
Fig. 3 Fig. 4
Fig. 5 Fig. 6
2.8. REPLACEMENT OF THE SPEED PROBE
WARNING
Before replacing the speed probe, ensure that the fans are fully stopped.
For the replacement of the speed probe, these steps should be followed:
c) Remove all screws M5 that hold the frontal cover of the plenum (Fig.2) and remove it.
The plenum can then be accessed, where the speed probe is located (Fig.3)
d) In case there is a box for the speed probe (Fig.4):
1 Remove the four screws of the speed probe box (Fig.4)
2 In case of replacing the speed probe including the box, remove the two M4
screws that hold it to the plenum (Fig.5) and disconnect the terminals 1,2,3
(Fig.6)
3 Loose the nut of the support of the speed probe until it can be released (Fig.7).
For totally removing this nut, it is necessary to take out first the extraction filter
(Fig.8) in order to access to the countersunk (Fig.9)
e) In case there is no box for the speed probe:
1 Remove the two screws M5 of the support of the speed probe (Fig.12) in order to
release the speed probe.
2 Disconnect the connector of the CPU (Fig.13)
f) Carry out the change.
For the installation of the new speed probe, all steps should be carried out in reverse order,
taking into account to place the speed probe with its hole in vertical position and the flag of
the speed probe must be looking upwards, because the flow should go through it
(Fig.11). The distance that should remain between the end of the probe and the sheet
should be 120 mm (Fig.10).
As last step, it is necessary to check the correct calibration of the cabinet.
Fig. 1 Fig. 2
Fig. 3 Fig. 4
Fig. 5 Fig. 6
Fig. 7 Fig. 8
Fig. 9 Fig. 10
Fig. 11
Fig. 12 Fig. 13
3. REPLACEMENT OF MECHANICAL ELEMENTS
WARNING
Before any service task ensure to perform a proper decontamination of the cabinet when
applicable as shown in user manual Annex 5.3.
After finishing any service task, it must be ensured that the cabinet is working properly,
according the checklist shown in chapter 4.
WARNING
Before performing the following service tasks, unplug the power plug of the cabinet in order
to avoid any risk of electrical shock.
It must be ensured the right fixing of the frontal cover by means of the two roods that are
supplied for such purpose.
REMARK
When it is required for the specific service task, the frontal cover must be raised or put back
to the closed position only when the glass is in the closed position. The contacts of the
frontal cover must be pushed back manually.
3.1. REPLACEMENT OF THE EXTRACTION FILTER
WARNING
Before proceeding to replace the filters, ensure that the fans are fully stopped.
For the replacement of the extraction filter, these steps should be followed:
a) Remove the two M5 screws that hold the frontal cover and lift it up, fixing it with the two
b) Remove all M5 screws that hold the frontal cover of the plenum (Fig.2) and remove it.
The plenum can then be accessed, where the filters are located.
c) Operate the two clamps (left and right) that hold the extraction filter (Figs.3&4), by
pushing or pulling the red levers they have.
d) Take out the filter by the frontal part (Fig.5).
For the installation of the new filter, all steps should be carried out in reverse order, checking
that it is perfectly supported all along the upper and the lower gaskets. As last step it is
necessary to re-calibrate the cabinet.
Fig. 1 Fig. 2
Fig. 3 Fig. 4
Fig. 5
3.2. REPLACEMENT OF THE IMPULSION FILTER
WARNING
Before proceeding to replace the filters, ensure that the fans are fully stopped.
For the replacement of the impulsion filter, these steps should be followed:
a) Remove the two M5 screws that hold the frontal cover and lift it up, fixing it with the two
b) Remove all M5 screws that hold the frontal cover of the plenum (Fig.2) and remove it.
The plenum can then be accessed, where the filters are located.
c) Operate the two clamps (left and right) that hold the impulsion filter (Figs.3), by pushing
or pulling the red levers they have.
d) Take out the filter by the frontal part (Fig.4).
For the installation of the new filter, all steps should be carried out in reverse order, checking
that it is perfectly supported all along the upper and the lower gaskets. As last step it is
necessary to re-calibrate the cabinet.
Fig. 1 Fig. 2
Fig. 3 Fig. 4
3.3. REPLACEMENT OF THE FRONTAL GLASS
WARNING
The frontal glass must be moved into the upper limit position before removing the nuts.
For the replacement of the frontal glass, these steps should be followed:
a) Detach the frontal mechanism from the cabinet. It can be then accessed (Fig.1)
b) Pull the glass up to the upper limit position. Remove the four M5 nuts that hold the
glass support in the frontal mechanism (Fig.2)
c) For the installation of the new glass, in case of having a pre-assembled glass group, all
steps should be carried out in reverse order.
d) In case of having to assembly the glass group, these steps are necessary:
a. Fix the glass to their metal supports with STRUCTURAL EPOXI ADHESIVE DP-190
3M SCOTCH WELD (Fig.4)
b. Screw the washers into the handles using M4x10 countersunk screws (Fig.5)
c. By means of the same ADHESIVE DP-190 3M SCOTCH WELD in all the surface of
the washers of the handles, fix them to the glass (Fig.6) by using previously
double side sticking tape in the center of the washers (Fig.3) in order they are
initially hold during the adhesive drying period of 24 h.
d. Check the operation of the closed position of glass micro-switch and adjust it if
necessary (Fig.7)
Fig. 1 Fig. 2
Fig. 3 Fig. 4 Fig. 5
Fig. 6 Fig. 7
3.4. REPLACEMENT OF THE SPRINGS OF THE GLASS
WARNING
The springs are assembled with pre-tension. Its assembly and disassembly must be made
carefully. Incorrect handling can result in damage to the cabinet and the technician.
For the replacement of the springs that compensate the glass weight, these steps should be
followed:
a) Remove the glass, as indicated in last chapter.
b) Spring groups can then be accessed (Fig. 1). As they are pre-tensioned, they must be
disassembled by carefully removal of the M5 nuts that hold them (Fig. 2).
c) Remove the M4 screw that supports the spring end (Fig. 6).
d) Remove the two M4 screws (Fig.3) of the spring cover in order to access to the part to be
replaced (Fig.4&5).
For the installation of the new spring, all steps should be carried out in reverse order taking
into account to screw firs the M4 screw that hold the end of the spring (Fig.6) before the M5
nuts that hold the spring groups (Fig.2).
Fig. 1 Fig. 2
Fig. 3 Fig. 4
Fig. 5 Fig. 6
4. FUNCTIONAL TEST
Depending on the repair task been made, following actions must be followed in order to
check the proper function of the cabinet:
Repair task zone Action To be checked
Switch on the cabinet to Check that the cabinet is switched on and
the mains supply and press it Works properly.
All the bottom ON/OFF. Check that the display is in green color
and no alarm appears (described in
chapter 5.2).
Switch on fans. Check that the fans work properly and
Zone 4, Zone 3 & CPU there is airflow.
Switch on fluorescents. Check that fluorescent can be switched

Zone 6, Zone 3 & CPU on.
Switch on sockets. Check voltage on sockets with a volt-

Zone 7, Zone 3 & CPU meter.
Zone 5, Zone 9, Zone 3 & Close glass and switch on Check that UV lamps can be switched on.
CPU UV lamp.
With cabinet in normal Check that the solenoid valve Works

working conditions, switch properly.
Zone 8 & CPU
on solenoid valve (only if
installed).
Zone 2, Zone 4, CPU & Calibrate the cabinet. See chapter 5.5 for calibration of the
when changing filters cabinet.
5. OPERATION AND CONTROL OF THE CABINET
5.1. START UP OF THE CABINET
To proceed to connect the cabinet:
- Connect the main supply to the cabinet by means of the power cord plug supplied by the
manufacturer
- Check that the aspiration and extraction air flow are free (Chapter 0, pos. 8).
- Carry out the appropriate installation tests (User Manual Chapter 3.6).
- Press the ON/OFF button (Annex A3 pos. 1 of the User Manual). After introducing the
password, the cabinet makes the following:
- It activates the fans.

- The regulation electronic system by microprocessor takes some seconds for stabilization
after the connection.
- The alarm of the insufficient laminar flow and insufficient extraction flow remains
activated until the cabinet reach working conditions.
- The gas tap button that activates the solenoid valve can only be activated once the
flow is stabilized. If case of any flow alarm, that solenoid valve will be disconnected.
To proceed to stop the cabinet, press ON/OFF button (Annex A3 pos. 1 of the User Manual)
that will lead to the Password enquiry (User Manual Chapter 4.5 point 1). After entering
the correct password, the cabinet will be switched off.
5.2. ALARMS
Display of the alarms: in the moment that a cabinet detects any mal-function the acoustic
and visual alarms are activated.
In the display will appear the information showing the corresponding messages and alarms.
• Alarms shown on the microprocessor display:
ALARM SHOWN ON THE DISPLAY

Excessive laminar down flow (>10%) HIGH LAM. FLOW
Insufficient laminar down flow (<-10%) LOW LAM. FLOW
Insufficient exhaust flow (<-10%) LOW EXH. FLOW
Front open / Glass out of working position WINDOW OPEN
Communication failure with redundant channel CHANNEL 2 COMM
Power supply interrupted POWER FAIL
No voltage in power area NO PPZ
• Other messages shown on the microprocessor display:
MESSAGE SHOWN ON THE DISPLAY
Germicidal lamp enabled GERMICIDE

Fans at half speed FAN ½
Test ROM memory ROM ERROR
Test EEPROM memory EEPROM ERROR
5.3. USER SCREENS TO BE USED IN MAINTENANCE OPERATIONS
In the control display is the general switch (Annex A3 pos. 1 of user manual).
A. On/Off Button (Annex 3 pos. 1):
• Pressing when the cabinet is turned off:

It goes to the Password Request screen. When entering the correct password (Chapter
4.5 point 1), it goes to the Main Operation screen.
• Pressing when the cabinet is turned on:

It goes to the Password Request screen. When entering the correct password (Chapter
4.5 point 1), the cabinet switches off.
B. Navigation buttons , and (Annex 3 pos. 2):
They move the cursor though all different function icons and select them. The icon
pointed inverts its colours. If the selected item is a numeric field, the navigation buttons
increase/decrease and validate the value.
C. Backlight graphic display (Annex 3 pos. 3):
It shows alarm messages, operating graphs, function icons and all the information
required in cabinet-user communication. The background colour changes to:
- Green: Working mode. All the flows are working fine and there are no alarms
activated.
- Red: Alarm mode. Any alarm has been activated or the cabinet is in unsafe mode. In
case of alarm, an acoustic alarm is activated together with the red light.
- Yellow: U.V. mode. The U.V. lamp is switched on.
- White-blue: Information mode. The cabinet is in safe mode although the flow is
disconnected.
Below is an explanation of the different user system screens to be used in maintenance

operations:
1. Main Operation screen.
Shows information about the velocity of the downflow as well as the clogging filter level. This
information is shown in the majority of the screens.
Activates/Deactivates fans.
Goes to Electric Control screen.
Enable the ECO-MODE: fans in half-

velocity, lights switched off and visual and
acoustic alarms activated (can be muted).
Goes to Utilities I Control screen.
2. Utilities I Control screen.
Provides access to the Disinfection screen,

prior to flow disconnection confirmation.
Access to Password Configuration screen.
Access to Maintenance Service screen.

Access to Utilities II Control screen.
2.1 Maintenance Service screen.
Access to Register screen.
Access to Language Selection screen.
Access to the Technical Service menu

(access restricted to authorised staff).
Back to Utilities I Control screen.
2.1.1. Register screen.
Shows information about the running hours of cabinet, fans, U.V. lamp and filters, together
with the last Service visit. Besides it allows the following:
Records the last revision done, where the

next forecasted revision is stored.
Shows the historical of revisions and

alarms.
Provides maintenance recommendations.

Back to Maintenance Service screen.
Last revisions screen Last alarms screen
5.4. CONTROL FUNCTIONS WITH TECHNICAL SERVICE PASSWORD
The access to the Technical Service menu can be done in two ways:
- By means of the Password Request screen (User Manual Chapter 4.5 point 1) by
introducing as password the key corresponding to the random number indicated in the
screen.
- Or with the icon for the Technical Service menu access.
Technical Service access screen:
The random numbers listing and their corresponding passwords is as follows:
Number Password Number Password

1 2421 11 7145
2 5832 12 3044
3 1294 13 6197
4 5119 14 8134
5 0547 15 4800
6 8168 16 3319
7 3632 17 0237
8 5901 18 5565
9 8533 19 2098
10 1942 20 4291
Once introduced the correct password, following screens can be shown:
1. Technical Service I screen.
Following actions can be carried out:
Go to the Fans Control screen.
Go to the Electrical Control screen.
Go to the Calibration screen.

Go to next Technical Service screen.
1.1 Fans Control screen.
Activate the fans.
De-activate the fans.
Activate the fans at low speed, lights and

alarms switched off.
Back to Technical Service I screen.
1.2 Electrical Control screen.
Connect/Disconnect the lights.
Enable/disable the plugs in the work zone.
Enable/disable the gas tap in the work zone

(if assembled).
1.3 Calibration screen.
This screen allows carrying out the calibration of the cabinet. Once accessed it will ask the
following things in this order:
- Extraction speed for a first calibration points.
- Laminar flow speed for a first calibration point.
- Extraction speed for a second calibration point.
- Laminar flow speed for a second calibration point.
Access to the calibration of the cabinet.
2 Technical Service II screen – Cabinet size.
Selects model size 3.
3 Technical Service III screen.
Go to the Set Points screen.
Go to the Reset Counters screen.
Go to the Settings screen.

3.1 Set Points screen.
Goes to the set points input screen.
Goes to the set points test screen.
Back to last screen.
3.1.1 Set Point screens.
Set Point Inflow Speed
Confirm the value of the selected field.
Reset the numeric fields.
Set Point Laminar Speed
Confirm the value of the selected field.
3.1.2 Test Set Points screen.
Confirm the test.
3.2 Reset Counters screen.
Let introduce the date after filter changing.
Let reset the fan hours counter.
Let reset UV lamp hours counter.
Go to the Set Points Test screen.
Back to Technical Service III screen.
3.3 Node-date screen.
Let select the node, in case of several

cabinets connected.
Let introduce the date.
Back to Technical Service III screen.
4 Technical Service IV screen – Cycle Test
To perform a cyclical functional test in the cabinet. Following actions can be carried out:
Selects the cyclical operation mode.
Resets the cycle counter.
Lets introduce the number of cycles.
5 Technical Service V screen – Engineering
Confirm the value of the selected

parameter. In case of:
- 2009 go to the software update menu by
using SD card.
- 6785 go to Engineering menu.
Back to user Menu.
5.1 Engineering Menu screen.
Confirm the selected parameter and

appears the next parameter to be entered.
Back to last parameters.
5.5. CALIBRATION OF THE CABINET
The purpose of this technical instruction is to regulate and check that the impulsion speed
flow and inflow are suitable to achieve product and user protection. To determine the speed
average as well as the uniformity range in the laminar flow zone and velocity through the
front aperture of the microbiological safety cabinet.
INSTRUMENTATION
• The tool to use is an omnidirectional anemometer with a support

• Unidireccional anemometer
• Meter, calculator, permanent felt-tip pen or squares template.
METHODOLOGY
1. Switch on the cabinet (the front glass should be in the working position and should not
be any alarm except the laminar flow or extraction).
2. In the upper side open the extraction regulator flow as shown in the pictures below. (Bio-
II-Advance 6 do not assembly these parts)
Bio-II-Advance 3 Bio-II-Advance 4 Bio-II-Advance 6
3. Go to Technical service Menu
4. Select size model (3,4,6). Adjust the set point extraction to 0.55 m/s and adjust the
impulsion set point to 0.35 m/s.
5. Go to calibration menu
6. Waiting at least 2 minutes. The cabinet is working now at constant fans voltage.
7. Take 5 velocity readings at 2.5 cm over the protective grills or at the level of the slot of
the exhaust, as indicated below. Enter the average exhaust velocity.
Unidirectional anemometer exhaust position
8. Then, measure the impulsion flow. (8 points in Bio-II-Advance 3, 8 points in Bio-II-
Advance 4 and 12 points in Bio-II-Advance 6, see calibration pattern in Annex A9.3 of
the User’s Manual to see the reading point situation and order for each model).
9. Place the anemometer at 270mm high of the shelves, over each point (take all readings
with the anemometer in the same direction and without covering any hole).
10. Take all the reading velocity needed and enter the average velocity
11. Once again, take 5 exhaust readings and enter the new exhaust average velocity
12. Enter the new average impulsion velocity measured in the same condition as points 8
and 9.
13. Entering the previous value the cabinet is adjusted to work at down flow velocity defined
in the set points. The display shows you the values of extraction flow, down flow, fan
voltage and inlet air velocity.
The only way we can change the inlet air velocity is through the extraction regulators
(see figures in point nº2). Opening, we increase the inlet air velocity and closing we
decreases de inlet air velocity.
IMPORTANT: IF WE CHANGE THE OPENING OF THE EXTRACTION REGULATOR,

WE WILL HAVE TO RECALIBRATE THE CABINET FROM THE POINT 5
14. If inlet air velocity is greater or equal than 0.55m/s, the cabinet is alright calibrated. If
the inlet air velocity is smaller than 0.55 m/s we need to increase the incoming air
speed, we will open the extraction regulator and recalibrate the cabinet from the point 5.
If the cabinet is alright calibrated we can switch off.
15. After calibrating the cabinet we have to check that it works correctly. Switch on the
cabinet (the front glass should be in the working position). Go to the technical service
menu where the fan voltage and air velocity appears. Wait at least 5 minutes.
16. Take 5 extraction air velocity values. The extraction average value of the 5 velocity
readings should be 0.55 ± display exhaust velocity.
V exhaust display ± 0.05 m/s = V average exhaust
17. Measure the impulsion air speed (take the values in the same points as calibration
mode). The average impulsion speed should be between 0.34 m/s and 0.36 m/s.
0.34≤Vaverage impulsion speed≤0.36
ACCEPTANCE CRITERIA
The cabinet is calibrated if it satisfy the permitted extraction speed and impulsion speed in
points 16 and 17.
If condition is not satisfied we proceed to calibrate the cabinet again from point 1.
6. RECOMMENDED INSTALLATION TEST
6.1. DOWNFLOW FILTERS INTEGRITY TEST
See Annex A9.1 of the User’s Manual.
6.2. EXHAUST FILTERS INTEGRITY TEST
6.3. DOWNFLOW AIR VELOCITY TEST
6.4. SET POINTS ALARM DOWNFLOW AIR VELOCITY TEST
See Annex A9.3.1 of the User’s Manual.
6.5. INFLOW AIR VELOCITY TEST
See Annex A9.4 of the User’s Manual
6.6. SET POINT ALARM INFLOW AIR VELOCITY TEST
See Annex A9.4.1 of the User’s Manual
6.7. SMOKE TEST
6.8. LIGHTING TEST
6.9. ACOUSTIC LEVEL TEST
7. ELECTRICAL DIAGRAMS
See Annex A7 of the User’s Manual.
8. COMPONENTS
8.1. OPTIONAL COMPONENTS
See paragraph 4.8 of the User’s Manual.
9. CLEANING
9.1. SUPERFICIAL CLEANING
9.2. FRONT GLASS DISINFECTION AND CLEANING
9.3. FORMALDEHYDE DISINFECTION
10. CERTIFICATES
See Annexes 10.1 and 10.2 of the User’s Manual.

SM BioIIAdvance EN 1013

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SM BioIIAdvance EN 1013

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SERVICE MANUAL

Microbiological Safety Cabinet

0. COMPONENTS DESCRIPTION ________________________________________ 4

2. Exhaust flexible tube

3. Laminar flow chamber or plenum

4. Laminar flow HEPA filter

5. Exhaust HEPA filter

7. Outside air inlet and protection barrier

1 Exhaust outlet. 6 Checking valve fans pressure.

2 Power supply. 7 Gas and vacuum connections

5 Checking valve for D.O.P. control

2.1. FUSE OF THE POWER SOCKET

a) Unplug the cord plug of the cabinet.

Fig. 3 Fig. 4 Fig. 5

F1 – Lighting Fuse 4A 250V

F3 – Fan fuse 10A 250V

a) Unplug the cord plug of the cabinet.

a) Unplug the cord plug of the cabinet.

a) Unplug the cord plug of the cabinet.

For the replacement of the UV light, these steps should be followed:

a) Unplug the cord plug of the cabinet.

In case the UV light design is assembled with clamps,

a) Unplug the cord plug of the cabinet.

a) Unplug the cord plug of the cabinet.

a) Unplug the cord plug of the cabinet.

Fig. 3 Fig. 4 Fig. 5

Switch on fluorescents. Check that fluorescent can be switched

Switch on sockets. Check voltage on sockets with a volt-

With cabinet in normal Check that the solenoid valve Works

5.1. START UP OF THE CABINET

To proceed to connect the cabinet:

- It activates the fans.

• Alarms shown on the microprocessor display:

ALARM SHOWN ON THE DISPLAY

• Other messages shown on the microprocessor display:

MESSAGE SHOWN ON THE DISPLAY

Germicidal lamp enabled GERMICIDE

A. On/Off Button (Annex 3 pos. 1):

• Pressing when the cabinet is turned off:

• Pressing when the cabinet is turned on:

B. Navigation buttons , and (Annex 3 pos. 2):

C. Backlight graphic display (Annex 3 pos. 3):

Below is an explanation of the different user system screens to be used in maintenance

1. Main Operation screen.

Goes to Electric Control screen.

Enable the ECO-MODE: fans in half-

Provides access to the Disinfection screen,

Access to Password Configuration screen.

Access to Maintenance Service screen.

2.1 Maintenance Service screen.

Access to Register screen.

Access to Language Selection screen.

Access to the Technical Service menu

2.1.1. Register screen.

Records the last revision done, where the

Shows the historical of revisions and

Provides maintenance recommendations.

Last revisions screen Last alarms screen

- Or with the icon for the Technical Service menu access.

Technical Service access screen:

The random numbers listing and their corresponding passwords is as follows:

Number Password Number Password