INSTRUCTION MANUAL

DISSOLVED OXYGEN
METER
314
SYSTRONICS
(AnIsO 9001 2015 Co.)
SYSTRONICS A Division of Systronics (India) Ltd.
1.0 INTRODUCTION
SYSTRONICS uC based Dissolved Oxygen Meter 314 is a battery operated portable
instrument for estimation of dissolved oxygen (D.O.) and temperature measurement in
water, wastewater and biological applications. Dissolved oxygen is measured in ppm (parts
per million) over a range of 0 to 20 ppm with 0.1 ppm resolution and Temperature is
indicated in degrees centigrade from 0 to +50.0° C. The dissolved oxygen measurement is
automatically temperature compensated for solubility of oxygen in water and permeability
of the probe membrane. Barometric and salinity compensation is automatic. Calibration,
LCD.
salinity, pressure entry and measurements are displayed by a
The probe supplied with the unit is a special Clark-type membrane covered polarographic
sensor. A PT100 probe is also supplied for temperature measurement. A thin, permeable
membrane stretched over the sensor isolates the sensor elements from the environment, but
allows oxygen to enter. When a polarising voltage is supplied across the sensor, oxygen
that has passed through the membrane reacts at the cathode, causing a current to flow.
1
2.0 SPECIFICATIONS
2.1 OxYGEN MEASUREMENT
Range 0-20 ppm.
Accuracy +1% of full scale
Resolution 0.1 ppm.
2.2 TEMPERATURE MEASUREMENT
Range O to +50° C.
Accuracy 0.5° C.
Resolution 0.1° C.
2.3 TEMPERATURE COMPENSATION
Within range of 0 to +50° C
2.4 SALINITY CORRECTION
O to 50.0 ppt.
2.5 BAROMETRIC PRESSURE CORRECTION
500 to 1499 mm Hg.
2.6 DISPLAY
1 line, 8 characters LCD with big font size.
2
2.7 POWER SUPPLY
One 9 V battery. (Eveready 216, Hi-Watt 6F22 OR equivalent) 9V
or adapter.
2.8 DIMENSIONS 170 x80x42.(mm)
2.9 STANDARD ACCESSORIES:
(a) DO probe
Cathode Gold
Anode Silver
Membrane FEP Teflon
Electrolyte 7.5% KCI saturated with AgCl.
(6) Temperature probe: PT100 based Probe
(Both Probes are placed on a single holder. See Fig. 1)
(c) Protective cover for the Probe (See Fig. 1)
(d) Instruction manual
2.10 SPECIAL FEATURES:
Hold function.
Backlight on/off.
Battery/mains - powered.
Automatic temperature compensation of DO value.
Non-volatile memory storage upto 50 reading.
3
(d) A/MI
Dual function increment or memory input.
Use to increase values of salinity, pressure and sample number or to save data of temp. and
DO value in memory.
(e) MR
Dual function decrement and memory recall.
Use to decrease values of salinity, pressure and sample number or to recall data of
temperature and DO value from memory.
( AIR CAL
Do air calibration automatic (setting of air calibration figure is not required).
( ZERO CAL
Do zero point calibration of probe.
(h) TEMP/D.0.
Toggles between Temperature or D.O. measurement.
()
Switch on backlight of LCD. Backlight will be automatically switch off after 5 seconds
(Approx.).
Note: Backlight has more power consumption.
5
 4.0 INSTALLATIONand CHECKOUT
The DO Meter 314 is a customer installed instrument. Before installation go through the
following section carefully:
4.1 GENERAL CONSIDERATION
Every unit is carefully packed when it leaves the factory. But it is advisable to check the
unit for any apparent damage. Do not operate the instrument if visual inspection is not
satisfactory.
4.2
4.2 POWER SUPPLY REQUIREMENT
This unit is battery operated and hence does not require mains outlet. It uses one 9 volt
small battery, e.g. Everyday 216, Hi-Watt 6F22 or equivalent (not supplied). While
inserting fresh battery please ensure correct polarities.
Instrument can work on 9V adapter.
Battery box is at the bottom of the instrument. The lid of battery box can easily be removed,
by removing two screws holding it.
5.0 OPERATING PROCEDURE
5.1 PROBE PREPARATION
5.1.1 MECHANICAL CONFIGURATION
The D.O. probe consists of 5 main parts, namely, (1) main stem (2) electrode cover (3)
sleeve (4) membrane and (5) Bakelite nut.
6
 The main stem is made of Perspex with gold electrode at lower end and silver ferrule fully
cable and a connector. The main
surrounding the gold. The upper end carries a connecting it
membrane assembly can be ftted to
stem has threads, so that electrode cover along with
by means of Bakelite nut.
The electrode cover is a thin cylinder that fits into lower half of main stem. Teflon, oxygen
of a rubber sleeve.
permeable membrane is held on to electrode cover by
means
5.1.2 ELECTROLYTIC SOLUTION
7.5% KCI (i.e. 7.5 gms KCI in 100 ml. distilled water). Slowly add AgCl crystals
Prepare
until AgCI stops getting dissolved.
5.1.3 ASSEMBLING THE PROBE
cover. Keep a piece of the (1" 1")
membrane x
First slide the Bakelite nut over the probe
and then push fit the sleeve over the tip
of the probe cover
over the tip of the probe cover flat and
electrode cover such that the membrane is
and then push fit the sleeve over the membrane and refit if
wrinkles on membrane. Remove the
tight. There should not be any the electrolyte solution and
detected. See Fig.3. Pour a few drops of
any wrinkles are with
membrane or near the sleeve. Now fill it completely
check for any leakage through the the probe
insert the main stem of the probe in
the solution. Keep it vertical and slowly flow out. Now tighten the
cover, which is filled
with the solution. Let the excess solution bubbles are
Make sure that there are no bubbles. If any
Bakelite nut over the main stem.
no bubbles are visible.
detected open the probe and refill, till
1
 Useful life of membranes depends on usage. Average replacement is 1-2 weeks. However,
should the electrolyte be allowed to
evaporate and an excessive amount of bubbles form
under the membrane, or the membrane become
damaged, thoroughly flush the electrode
with KCl and install a new membrane. Also
replace the membrane if erratic readings are
observed or calibration is not stable.
The gold cathode should always be
bright and untarnished. To clean, wipe with a clean lint-
free cloth or hard paper. NEVER USE ANY FORM OF ABRASIVE OR
CHEMICAL.
Rinse the sensor several times with KCI, refill, and install a new membrane.
5.2 PREPARING THE INSTRUMENT
1. Switch on the instrument. (Power switch is located on the left
side)
Attach the prepared DO probe to the PROBE connector.
3 (Located at rear side)
Allow15 minutes to polarise the probe. Depolarise whenever the instrument has been OFF
or the
probe has been disconnected.
Note: Here after the DO probe and Temperature probe combined will be referred as PROBE.
5.3 AIR CALIBRATION
Experience has shown that air calibration method is quite reliable, yet far simpler than the
other methods. Air calibration method is given below:
1. Place the PROBE in moist air. For this, PROBE can be wrapped loosely in a
damp cloth
taking care the cloth does not touch the membrane. Wait approximately 10 minutes for
temperature stabilisation
8
2 After 10 minutes press mmHg key to see and/or change atmospheric pressure. You can
change pressure by up (A) and down ( ) key. Press ENTER key to store new value and/or
calibration if you neglect
exit from pressure mode. Old value is taken in measurement or
this step. (You can use table 1 to convert from altitude value to mmHg.)
Press SAL (ppt) key to see and/or change salinity in parts per thousand You
3 (ppt). can
change salinity by up (A) and down (V) key. Press ENTER key to store new value and/or
exit from salinity mode. Old value is taken in measurement orcalibration .if you neglect this
step.
4 Press AIR CAL key to do air calibration automatic. LCD will display AIR CAL message.
Wait for 30 second.
5. Press ENTER key to do calibration. And LCD display new ppm value according to
temperature.
6 PressTemp/DO to escape AIR Calibration.
5.4 ZERO CALIBRATION
1. Add sodium sulphate, NaSOs, crystals approx. in proportion of 2 mg per litre of
Distilled water and trace of cobalt chloride CoCl2.
should
2. Place probe in this solution. This will bring the DO in solution to zero. The reading
comedown approximately 0.5 ppm and stabilises afterapproximately 5 minutes.
3 Press ZERO CAL key. LCD shows "ZERO CAL" message. Wait for 30 seconds.
A. Press ENTER key to confirm Zero calibration. The reading must be set zero for good probe,
otherwise indicates "ERROR" message.
9
 Press TEMP/DO to escape Zero calibration.
5.5
6.
5. Calibration is not possible if
temperature is out of range.
Note: If ERROR
to message is displayed then
Set
1. With
It
prevent
stirring.
may be possible that
2. the temperature is out of range
Air
stabilise.
calibration
the was not done
proper
3 It may be possible
salinity that measurement value of zero ppm solution is greater. (>1.5 ppm)
The
depletion
Samples
instrument
Read is
instrument now calibrated. Calibration can be distorted by
the membrane, and
or drying out of the
physical shock, touching
of electrolytic. Check the calibration periodically.
DISSOLVED OXYGEN
should
DO
MEASUREMENT
dissolved
pressure
1.
calibrated,
near
be
value
the
oxygen.
place
of
continuously
membrane
the
the
Note: Old value of pressure and salinity is taken in measurement if it
is not changed in new
measurement. sample
PROBE
of
Note: that "true" stirred
the
atmospheric pressure is as read on a barometer. If atmospheric pressure is
unknown, the local altitude may be substituted. under in
by
the
PROBE.
test.
10 stirrer
sample
Allow
to
during
seeecsasaelaal
 5.6 MEMORY FUNCTION
5.6.1 DATA INPUT
1. Ensure the meter is in appropriate measurement mode.
2 When reading become stable, press MI key. So LCD will show you message Smpl 01
". In message 01 indicate sample
number
3 Sample number can be changed by A/MI and V/MR key.
Press ENTER/HOLD key to store value of DO and temperature as displayed sample number
and display returned original measurement mode.
5.6.2 MEMORY RECALL
1 Ensure the meter is in appropriate measurement mode.
2 Press /MR key to recall stored value. Display will show you sample number by message
Smpl 01 ". In LCD message 01 indicate sample number.
Sample number can be changed by A/MI and V/MR key.
A. Press ENTER/HOLD key to recall value of DO and Temperature stored at set sample
number.
5. Now display toggle between message "Mem mode" and recalled data Like "14.6 ppm" OR
25.0° C
6. Pressing Temp/D.O. key in memory mode toggles recalled data.
7. To exit from memory mode press/MR key again.
8. Repeat above procedure to recall another data in LIFO (last in first out) system.
12
6.0 ADDITIONAL INFORMATION
The DO Dissolved oxygen (DO) is the small amount of oxygen that is dissolved in the
water. This oxygen is vital to fish, other aquatic animals, microorganisms and plants, which
depend upon it for the process of respiration, i.e. to "breathe'. Maintenance of healthy and
diverse aquatic ecosystems depends on oxygen levels being maintained at consistently high
levels. Reductions in DO levels result in loss of the more sensitive species. At very low DO
levels, only very few species may be present.
Level is also a useful indicator of water quality. It can tell us how a water body is behaving
and it can indicate the presence of certain pollutants, particularly organic matter (matter that
has come plants and animals e.g. fallen leaves, animal manure, sewage effluent).
from
In water
quality applications, such as aquaculture (including fish farming) and wastewater
treatment, the level of DO must be kept high. For aquaculture if the D0 level falls too low
the fish will suffocate. In sewage treatment, bacteria decompose the solids. If the DO level
is too
low, the
bacteria will die and decomposition ceases; if the DO level is too
energy is wasted in the aeration of the water. With industrial applications including boilers,
high,
the make-up water must have low D0 levels to prevent corrosion and boiler scale build-up
which inhibits heat transfer.
13
7.0
MANUFACTURER'S WARRANTY
This instrument is warranted for a period of twelve months from the date of delivery against
defects in materials or workmanship, other than valves, photocells, batteries, semiconductor
devices, meters, cells and conductivity cells. During the warranty period, liability is our
limited to the repair replacement, at option, of the instrument parts thereof found
to be defective.
or our or
8.0 SERVICE AFTER SALES
Utmost care is taken in the design, manufacture and inspection of every SYSTRONICS
instrument to ensure trouble-fre service over long periods. However, in case of
unsatisfactory performance of any of our instruments, please contact our Distributors for
your area through whom the was purchased, under intimation to us. All our Distributors
have Service Departments manned by Service Engine rs.
14
 KNURLED SCREW
DISSOLVED OXYGEN PROBE
TEMPERATURE PROBE
PROTECTIVE COVER
FIG:-1 PROBE
 PROBE STEM-
NUT-
)
MEMBRANE-
,
PROBE CoVER
sLEEVE
FIG:-2 DO PROBE ASSEMBLY
9 VOLT ADAPTOR SOCKET
DO/TEMP PROBE sOCKET
DO METER 314
SLIDE SWITCH
(POWER ON) SYS-314 - LCD
KEY PAD
/zERO
CAL
HOLD
ENTER
FIG:-4 ISOMETRIC VIEW