In the name of God

Biochemistry Lab WORK REPORT

Name of course: Discipline Biochemistry

Field & Education level: Medical Stu. _ Term 2

Bench number (Group members): 3
‫ سید اوختای حسینی‬،‫ مهیار کاظمی‬،‫ المیرا محبی‬،‫ مبینا کیمیا قلم‬،‫ آیناز جدیری‬،‫نرجس میرزایی‬

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Test title: Liver Function Tests
The purpose of the experiment:
The quantitative determination ALP
of Alkaline Phosphatase Total Activity in solution
The basis of the test: Colorimetric determination of the ALP activity as follows:
p-Nitrophenyl phosphate (Reagent) p-Ntrophenoxide + Pi
[Test/Standard sample]

pNPP is colorless but pNP has a strong absorbance at 405 nm.

The results are based on the total absorbance per unit of time.

Test procedure:

ALP [samples] (20 µL) + pNPP [reagent] (1000 µL) <--> pNP [Dyed sample]

Mix and insert the cuvette into the spectrophotometer at 405 nm against air blank.
Read inidial absorbance after 1 minute and record absorbances at 1 minute intervals thereafter for 3
minutes.
Calculate the average absorbance per minute and multipy by factor 2.757 for results in IU/L.

Minute 1 st 2 nd 3 rd 4 th DA/min C s/t

Standard a. 1.309 1.440 1.950 1.400 1.52475 254×2.757

Test a. 1.286 1.352 1.412 1.233 1.32075 ?

Test result:
Ct= 606.5 IU/L