LABORATORY EQUIPMENT

• For sterility providing:

Autoclave, clean air technology (laminar crossflow
benches), equipment for instrument sterilization, sterile one
time use laboratory dishes, autoclavable laboratory dishes,
test tubes, steward bottles, etc.
• For all methods:
Equipment for water deionizing, thermostats, pH meters,
magnetic mixer, balance, refrigerator, computer,
greenhouse, etc.
• Depending of used method:
Microscope (1000X), zoom microscope (50X), binocular,
centrifuge, blenders, vortex, etc.
Human cell and tissue cultures
 Plant tissue cultures
• Cultures of cells or tissues
grown in aseptic conditions.
• First experiments1902. g. Gottlieb Haberlandt

• First plants regenerants1946.g.

(meristem cultureTropaelium majus)
• Second part of 20. cent.
 Mediums
Liquid Solid
•Micro-elements
•Macro-elements
•Vitamins
•Hormones
• different biologically active substances
(salts, extracts, anti-oxidants,...)
•Sugars (sucrose, maltose, glucose...)
•Jelly (agar, agarose, fit gel, ficols,...)
•Dejonized water
 MS medium (Murashige and Skoog, 1962)

Makroelementi (mg/l)
KNO3 1900
NH4NO3 1650
KH2PO4 170
MgSO4 x 7H2O 370
CaCl2 x 2H2O 440
Mikroelementi
FeNa2 x EDTA 37.3
MnSO4 x 5H2O 22.3
H3BO3 6.2
ZnSO4 x 7H2O 8.6
CoCl2 x 6H2O 0.025
CuSO4 x 5H2O 0.025
Na2MoO4 x 2H2O 0.25
KI 0.83
Citi komponenti
myo-Inositol 100.0
Tiamina HCl 0.4
Nikotīnskābe 0.5
Piroxidina HCl 0.5
Saharoze 30.0 g/l
Agars 6.0 g/l
•Tabaka Arabidopsis
(Arabidopsis thaliana)
Protoplast culture
 FAST PROPAGATION OF A
ARTICULAR GENOTYPE

Micropropagation
Use of bioreactors
Restoring of genetic resources
Embryo culture, micropropagation

Plant obtaining from 16.-44. years old seeds

Cells culture
Calli culture
•Somaclonal variation
•Mutagenesis
•Gene transfer
Somaclonal variation
 Flax calli development
100

regeneration zones (%)

Leaves calli with
80
Vega 2
60
Lirina
40 Rigaer B

20

0
Control Fenoxan Ambiol Diludin AV 153
Antioxidants
 Use of double haploids (DH)

• Breeding
(Obtaining of homozygous lines )

• Development of genetics maps

DH obtaining with use of embryo culture
DH obtaining
Hybrids

pollen, anther, ovary,

embryo culture

Haploids

Double haploids
Influence of stress

Clement et al.,2006
Microspore (pollen) culture
Anther culture
Latvian Academy of Sciences as one of the "Latvian
scientific achievements in 2011" practically applied
sciences, has nominated the result of cooperation of
State Stende Plant Breeding Institute and Plant
Genetics Laboratory of Institute of Biology of Latvian
University, new spring wheat variety 'ROBIJS'

http://www.stendeselekcija.lv/?subaction=showfull&id=1325928681&archive=
Protoplast culture
Cells culture obtaining of genetical changes

Calli culture

Meristem culture fast propagation of a

particular genotype
Pollen culture
breeding
Anther culture producing of haploids,
doubled haploids mapping
Ovary culture

Embryo culture wide hybrids