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Cell Biology Apoptosis Intracellular NFkB RelA (p65) Share by email

NF kappaB p65 ELISA Kit (ab176648)

Submit a review Submit a question References (22) Datasheet SDS Protocol Booklet

Key features and details

• One-wash 90 minute protocol

$670
Product size
• Sensitivity: 5 µg/ml
1 x 96 tests

• Range: 6 µg/ml - 600 µg/ml

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• Sample type: Cell Lysate, Tissue Homogenate

• Detection method: Colorimetric Order now and get it on Monday

January 15, 2024
• Assay type: Semi-quantitative
Custom quote | Bulk request
• Reacts with: Mouse, Human

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Product name
NF kappaB p65 ELISA Kit
See all NF-kB p65 kits

Detection method
Colorimetric

Precision
Intra-assay

Sample n Mean SD CV%

HeLa extract 6 2.8%

Inter-assay

Sample n Mean SD CV%

HeLa extract 3 8.7%

Sample type
Cell Lysate, Tissue Homogenate

Assay type
Semi-quantitative

Sensitivity
5 µg/ml

Range
6 µg/ml - 600 µg/ml

Assay time
1h 30m

Assay duration
One step assay

Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat

Product overview
Abcam’s NFκB p65 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for
the semi-quantitative measurement of NFκB p65 protein in Human and mouse cells.

The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated
detector antibody which immunocapture the sample analyte in solution. This entire complex (capture
antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating
the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix.
After incubation, the wells are washed to remove unbound material. TMB substrate is added and during
incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop
Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount
of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading,
development of TMB can be recorded kinetically at 600 nm.

As of October 2019, this kit was reformulated with new antibodies to maintain continued long term
supply.

Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products
that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and
any other relevant authorisations, for their intended uses.

Platform
Microplate

Properties +
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Datasheets and documents +
References (22) +
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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References (14) References (22)

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