Bhartiya

Public School, Sikar

Near sanwali circle,
jaipur Bikaner bypass, sikar

Chemistry
Project
Session : 2020-2021
A Project Report On
“digestion of starch by salivary amylase

and effect of temperature

and pH on it”

Submitted by Samuel Kumar

Class – XII th (Science)
Roll no. (cbse) – ______
Under the Guidance of
Mr. Amit Bedwal Sir
PGT (Chemistry)
 Table of Contents
 Certificate

 Declaration

 Acknowledgement

 Aim of Project

 Objective of the Project Report

 Introduction

 Apparatus Required

 Procedure

 Observation

 Conclusion

 Precaution

 Bibliography
 Bhartiya Public School, Sikar

Certificate

This is to certify that _______________________ student of class XIIth (Sci.) has

successfully prepared the report on the Project entitled “To study the digestion of

starch by salivary amylase and effect of temperature and pH on it” under the guidance

of

Mr. AMIT BEDWAL & Mr. PUSHPENDRA (PGT Chemistry).

The report is the result of his efforts & endeavours.

The report is found worthy of acceptance as final

Project report for the subject Chemistry

of class XIIth (sci.).

Signature of Chemistry Teacher Signature o f External Examiner

--------------------------- --------------------------

Signature of Principal
---------------------------

Declaration
I hereby declare that the project work entitled

“ To study the digestion of starch by

salivary amylase and effect of temperature

and pH on it ” , submitted to Department of chemistry,

Bhartiya Public School, Sikar is prepared by me.

Student Name : _________________________

Class : XIIth Science)

 Acknowledgement

I would like to express a deep sense of thanks and gratitude

to my project guide Mr. Amit Bedwal & Mr. Pushpendra for

guiding me immensely

through the course of the project. He always envinced keen

intrest in my project. His constructive advice & constant

motivation have been responsible for the successful completion

of his project.

My sincere thank goes to our principal sir for his co-ordination

in extending every possible support for the completion

of this project.

I must thanks to my classmates for their timely help and

support for completion of this project.

Last but not the least, I would like to thank all those who had

helped directly or indirectly towards the

completion of this project.

Student Name : ________________________________

Class- XIIth Science)

AIM

“ To study the digestion of

Starch by salivary amylase
and
effect of temperature
and pH on it. ”
Trusted by
 Objective of the Project Report

The main objective of this chemistry project report is

“To study the digestion of starch by salivary amylase
and effect of temperature and pH on it”
1. To study the digestion of starch by saliva.
2. To study the effect of temperature on the digestion of
starch by saliva.
3. To study the effect of pH on the salivary digestion of
starch.
 Introduction

Every health book insists on the chewing of food. The act of chewing stimulates
the excretion of saliva. Saliva mixes up with the food and helps its digestion. That
is, the enzyme ptyalin or amylase present in human saliva hydrolyzes the big
molecules of food into many molecules.

For example, starch into mono-saccnaricies maltose and glucose; proteins into
amino acids and fats into fatty acids

and glycerol.

Thus saliva not only helps in digestion of food but also convert into energy
generating substances. Further, enzyme

and their activity are very sensitive to temperature and pH. Even a slight variation
in these two factors, can disturb the action of enzymes. In other words, digestion of
food by salivary amylase is also effected by temperature and pH and can be
verified experimentally.

For example, hydrolysis of starch can be verified by testing it with iodine solution.
Starch forms blue coloured complex with iodine. If no starch is present in a system
it will not give blue colour with iodine.
 Apparatus Required

The requirements for experiment of chemistry project report Are as follow:

1. Test tubes

2. Test tube stand

3. One dropper

4. Beaker

5. Stop watch

6. Starch

7. Iodine solution

8. Thermometer

9. Dilute HCl solution

10. Dilute NaOH solution

 Experiment 1
Aim:- To study the digestion of starch by saliva

Procedure for Chemistry Experiment 1 is:

1. Collection of Saliva:- Rinse mouth thoroughly with cold water and ensure that it does not contain any food
particles. Now take about 250ml of lukewarm water in the mouth and keep for about three minutes so that
saliva mixes up well with it. Spit this into a beaker. Filter, if there is any suspended impurity clear filtrate is
saliva solution and contains enzyme ptylin.

2. Preparation of starch solution:- Take about 0.5g of starch in 100ml beaker and add enough water to

make a paste. Dilute the paste by adding 50ml water and boil for

about 5 minutes.

3. Digestion of starch :- (a) Take 5ml of the starch solution in a test tube. Add 2ml of saliva

solution into it. Mix the solution well by shaking the tube carefully

and start a step watch.

(b) After one minute, take out two drops of the mixture solution from

the test tube with the help of dropper and transfer it into another test

tube containing about 1ml of 1% iodine solution. Note the colour

produced, if any.

(c) Repeat this test after every one minute taking two drops of the
mixture solution and fresh 1% iodine solution continue until the test
shows no blue colour.

(d) Record the time and blue colour intensity.

Observation

Time Passed after

Mixing 1 Minute 2 Minute 3 Minute 4 Minute

Colour Intensity
Deep Blue Blue Light Blue No Blue

Absence of blue colour on addition to iodine solution means

absence of starch in the mixture solution. That is whole of

the starch has got digested or hydrolysed.

Conclusion

Starch gets hydrolysed by salivary amylase

 Experiment 2
Aim:- To study the effect of temperature on the digestion of starch by saliva.

Procedure for Chemistry Experiment 2 is:

1. Take three test tubes and label these 1,2, and 3.

2. Take 5ml of the starch solution, 2ml of the saliva solution and 5ml of water in each test tube. And
shake the test tubes carefully.

3. Place test tube number 1 in water at room temperature, test tube number 2 in a beaker containing
water at 50°C and test tube number 3 in boiling water.

4. After 5 minutes, observe the colour change on mixing two drops of the mixture of every tube with one
ml of 1% iodine solution. Note the intensity of blue coloured form