GNR Stool Pathogens Lactose Negative Flowchart

H2S TSI or KIA reaction. See TSI Reactions ID

H2S K/A chart. Perform spot tests from BAP
H2S K/A No subculture only. A/A K/A
A/A Gas Gas
A/A Gas K/A Gas

H2S (-) (acid

H2S (+)* with
K/K Alkaline / no butt)* with A/A,
A/A, K/A Gas,
change (K/K) A/A Gas, K/A,
K/A No Gas
K/A Gas

Urea (+) or oxidase (+)* Urea (-) and

NSF See page 2
oxidase (-)*

NSF
PYR (-)* PYR (+)* This chart is for lactose negative or H2S positive
microorganisms: Aeromonas, Salmonella, Shigella,
Vibrio, and Yersinia. Rare pathogenic E. coli may be
lactose negative. They will produce gas in a TSI. Use
other ID methods.

Indole (+)* Indole (-)* NSF NSF = normal stool flora

r/o Edwardsiella
r/o = rule out
1
= Hafnia spp. can key out as Salmonella and can
agglutinate with Shigella or Salmonella antisera, but it is
o
VP positive at 25 C
r/o Salmonella
* = See Biochemical Tests for Gram Negative Organism
ID Job aid for positive and negative result reference.

Laboratory Training Branch

GNR Stool Pathogens Lactose Negative Flowchart

A/A K/A
A/A Gas K/A Gas

H2S (-) (acid butt)* with

A/A
A/A, A/A Gas, K/A, K/A Gas
A/A Gas

Oxidase (+)* Oxidase (-)*

Gas and urea positive, or gas and indole

r/o positive, or, for KIA only, acid/acid with or
Aeromonas, without gas* (A/A, or A/A gas)
Vibrio, and
Plesiomonas
Yes No

NSF PYR

K/A
K/A Gas PYR (-)* PYR (+)*

Urea
Urea (-)* For TSI, Gas (+)* or Nonmotile at
(+)*
acid/acid motile at 35oC and gas (-);
with or 35oC r/o Yersinia*
Alkaline / acid (K/A,
without gas NSF
or K/A Gas)
(A/A, or
A/A Gas) NSF
Indole (+)
Indole (-)*
Gas (-)*
NSF
r/o
Gas (+)* Note1 Gas (-)*
Shigella

r/o r/o
Salmonella Salmonella
and Shigella

Laboratory Training Branch