10th Advances in Stem Cell Biology

WEEK 1-JUNE 26 TO JULY 01 2023

Monday June 26 2023

09:00 - 09:30 Welcome Coffee - Administrative instructions Education Center - Virginie Ponticelli,
Sylvie Malot
(Institut Pasteur – Paris - France)
09:30 - 11:00 General course introduction Shahragim Tajbakhsh
(Institut Pasteur – Paris - France)
Laure Bally Cuif
(Institut Pasteur – Paris - France)
11:00 - 11:40 Participants' presentations (5 min/participant max.) Participants 1-7
COURSE 1: REPROGRAMMING OF HUMAN SOMATIC CELLS
11:40 - 12:40 Lecture 1 : Laurence Daheron
Pluripotent stem cell culture: Overview (Harvard Stem Cell Institute, Boston, USA)
12:40 - 13:40 Lunch Break
13:45 - 14:00 Safety instructions Théo Putigny
(Institut Pasteur – Paris - France)
14:00 - 18:30 Course 1 Laurence Daheron
Hands-on and analysis (Harvard Stem Cell Institute, Boston, USA)
Frank Yates
16:00 – 16:45 (Lecture) (SupBiotech, Villejuif, France)
Culture systems for human pluripotent stem cell Michel Cohen-Tannoudji
(Institut Pasteur, Paris, France)
18:30 - 19:10 Participants' presentations (5-7 min/participant) Participants 8-14
20:00 Dinner with experts (L’Authentique)

Tuesday June 27 2023

COURSE 1- REPROGRAMMING OF HUMAN SOMATIC CELLS
09:00 - 11:00 Course 1 Laurence Daheron
Hands-on and analysis (Harvard Stem Cell Institute, Boston, USA)
Frank Yates
(SupBiotech, Villejuif, France)
Michel Cohen-Tannoudji
(Institut Pasteur, Paris, France)
11:00 - 12:30 Lecture : Michele De Luca
Epithelial stem cells in cell and gene therapy (University of Modena and Reggio Emilia, Italy)
12:40 - 14:15 Lunch Break
14:15 - 18:00 Course 1 Laurence Daheron
Hands-on and analysis (Harvard Stem Cell Institute, Boston, USA)
14:15-15:00 (Lectures) Frank Yates
• Pluripotent stem cell characterisation (SupBiotech, Villejuif, France)
• Introduction to gene editing in Human pluripotent Michel Cohen-Tannoudji
stem cells (Institut Pasteur, Paris, France)
18:00 - 18:30 Lecture 2 : Frank Yates
Induced Pluripotent Stem Cells Applications: (SupBiotech, Villejuif, France)
from bench to organoids

3 June 26 – July 7, 2023, Institut Pasteur, Paris France

 10th Advances in Stem Cell Biology

Wednesday June 28 2023

09:00 - 10:30 Lecture: Cédric Blanpain
Epithelial stem cell fate and potential (UBL – Bruxelles - Belgium)
10:30 –11:00 Coffee break and discussion
COURSE 2 – ORIGIN OF BLASTEMA CELLS DURING REGENERATION IN THE ANNELID PLATYNEREIS DUMERILII
11:00 - 13:30 Course 2 Eve Gazave
Hands on and analysis (Institut Jacques Monod – Paris - France)
Loïc Bideau
(Institut Jacques Monod – Paris - France)
13:30 - 14:30 Lunch Break
COURSE 3 - ANALYSING STEM-CELL LIKE BEHAVIOURS IN VIVO USING BIRD EMBRYO AS A MODEL SYSTEM
14:30 - 18:30 Course 3 Jérôme Gros
Embryo collection, electroporation and ex vivo (Institut Pasteur – Paris - France)
culture Olinda Alegria-Prevot
(Institut Pasteur – Paris - France)

Thursday June 29 2023

COURSE 3 - ANALYSING STEM-CELL LIKE BEHAVIOURS IN VIVO USING BIRD EMBRYO AS A MODEL SYSTEM
09:00 - 11:00 Course 3 Jérôme Gros
Observation/imaging of GFP electroporated chick (Institut Pasteur – Paris - France)
embryos Olinda Alegria-Prevot
(Institut Pasteur – Paris - France)
11:00 - 12:30 Lecture : Shahragim Tajbakhsh
Stem and niche cells in development and (Institut Pasteur – Paris - France)
regeneration
12:30 - 13:30 Lunch Break
COURSE 4 – ISOLATION OF SKELETAL MUSCLE STEM CELLS ON SINGLE MYOFIBRES
13:30 - 18:30 Course 4 Barbara Gayraud-Morel
Hands on and analysis (Institut Pasteur – Paris - France)
Shahragim Tajbakhsh
(Institut Pasteur – Paris - France)
Brendan Evano
(Institut Pasteur – Paris - France)

Friday June 30 2023

09:00 - 10:30 Lecture : Marc van de Wetering
Intestinal stem cells (Prinses Maxima Centrum - Utrecht- NL)
10:30 –11:00 Coffee break and room change
11:00 - 12:30 Lecture : Meritxell Huch
Tissue derived organoid cultures (Max Planck Institute - Gesellschaft -
Germany)
12:30 - 13:30 Lunch Break
13:30 - 14:00 Group picture (Pasteur Museum)
COURSE 2 – ORIGIN OF BLASTEMA CELLS DURING REGENERATION IN THE ANNELID PLATYNEREIS DUMERILII
14:00 - 14:30 Course 2 Eve Gazave
Hands on and analysis (Institut Jacques Monod – Paris - France)
Loïc Bideau
(Institut Jacques Monod – Paris - France)

4 June 26 – July 7, 2023, Institut Pasteur, Paris France

 10th Advances in Stem Cell Biology
COURSE 5 - MOUSE INTESTINAL ORGANOIDS: HOW TO MAKE MINIGUT
14:30 - 19:00 Course 5 Giulia Nigro
Crypts extraction and organoid culture (Institut Pasteur – Paris - France)
Marc van de Wetering
(Prinses Maxima Centrum – Utrecht - NL)
Laetitia Aymeric
(INSERM/CHU – Angers - France)
Aline Stedman
(IBPS – Paris – France)
20:00 Dinner with experts (To be defined)

Saturday July 01 2023

09:30 - 11:00 Lecture : Naomi Moris
Using gastruloids and other 3D models to (The Francis Crick Institute - London – UK)
explore principles of mammalian development
COURSE 6 - STUDYING CELL FATE DECISIONS IN ESCS
11:00 - 13:00 Course 6 Alfonso Martinez Arias
Generation of Aggregates (Universidad Pompeu Fabra - Barcelona -
Spain)
David Turner
(University of Liverpool - UK)
COURSE 5 - MOUSE INTESTINAL ORGANOIDS: HOW TO MAKE MINIGUT
13:00-14:00 Course 5 – 2 groups in parallel – Giulia Nigro
Observation of crypts (with or without fluorescent (Institut Pasteur – Paris - France)
microscope) Marc van de Wetering
(Prinses Maxima Centrum – Utrecht - NL)
Laetitia Aymeric
(INSERM/CHU – Angers - France)
Aline Stedman
(IBPS – Paris – France)

5 June 26 – July 7, 2023, Institut Pasteur, Paris France

 10th Advances in Stem Cell Biology

WEEK 2 – JULY 03 TO JULY 08 2023

Monday July 03 2023

09:00 - 11:15 Course 2 Eve Gazave
Hands on and analysis (Institut Jacques Monod – Paris - France)
Loïc Bideau
(Institut Jacques Monod – Paris - France)

11:30 - 13:00 Lecture: Matthias Lutolf

Emerging technologies to probe and manipulate (École Polytechnique Fédérale de Lausanne,
stem cell fate and self-organisation CH)
13:00 - 14:00 Lunch Break
COURSE 6 - STUDYING CELL FATE DECISIONS IN ESCS
14:00 - 14:30 Course 6 Alfonso Martinez Arias
Generation of Aggregates (Universidad Pompeu Fabra - Barcelona -
Spain)
David Turner
(University of Liverpool - UK)

COURSE 7 - HEMATOPOIETIC STEM CELLS IN MOUSE AND MAN

14:30 - 18:30 Course 7 Ana Cumano
Hands-on and Lecture : (Institut Pasteur – Paris - France)
Hematopoiesis: from fetal liver to bone marrow

COURSE 1- REPROGRAMMING OF HUMAN SOMATIC CELLS

18:30 - 19:00 Course 1 Frank Yates
Analysis (SupBiotech, Villejuif, France)
Michel Cohen-Tannoudji
(Institut Pasteur, Paris, France)

Tuesday July 04 2023

COURSE 7 - HEMATOPOIETIC STEM CELLS IN MOUSE AND MAN
09:00 - 11:00 Course 7 Ana Cumano
Hands-on Analysis (Institut Pasteur – Paris - France)

COURSE 8 - CRE-LOX TRACKING OF ADULT NEURAL STEM CELLS IN THE ZEBRAFISH BRAIN
11:00 - 11:30 Course 8 Laure Bally-Cuif
Introduction (Institut Pasteur – Paris - France)
Tanya Foley
(Institut Pasteur – Paris - France)
Isabelle Foucher
(Institut Pasteur – Paris - France)
Sara Ortica
(Institut Pasteur – Paris - France)

11:30 –11:45 Room change

11:45 - 13:00 Lecture Fabian Theis
(HelmholtzZentrum München, Neuherberg,
Germany)

6 June 26 – July 7, 2023, Institut Pasteur, Paris France

 10th Advances in Stem Cell Biology
COURSE 8 - CRE-LOX TRACKING OF ADULT NEURAL STEM CELLS IN THE ZEBRAFISH BRAIN
13:00 - 17:00 Course 8 - 2 groups in parallel
Hands on and analysis Laure Bally-Cuif
(Institut Pasteur – Paris - France)
Tanya Foley
Lunch (Institut Pasteur – Paris - France)
13:00-13:30 (Group2) Isabelle Foucher
14:00-14:30 (Group1) (Institut Pasteur – Paris - France)
Sara Ortica
17:00 - 18:30 Course 8 (Institut Pasteur – Paris - France)
All – Interpretation, Discussion
COURSE 6 - STUDYING CELL FATE DECISIONS IN ESCS
18:30 – 19:30 Course 6 Alfonso Martinez Arias
Applying Stimuli (Universidad Pompeu Fabra - Barcelona -
Spain)
David Turner
(University of Liverpool - UK)

Wednesday July 05 2023

COURSE 5 - MOUSE INTESTINAL ORGANOIDS: HOW TO MAKE MINIGUT
09:00 - 10:30 Course 5 Giulia Nigro
Observation of crypts (with or without fluorescent (Institut Pasteur – Paris - France)
microscope)

10:30 - 12:00 Lecture: Leila Périé

Blood cell production by the numbers (Institut Curie – Paris – France)

12:00 - 13:30 Lunch Break

COURSE 4 – ISOLATION OF SKELETAL MUSCLE STEM CELLS ON SINGLE MYOFIBRES
13:30 - 15:00 Course 4 Barbara Gayraud-Morel
Hands on and analysis (Institut Pasteur – Paris - France)
Shahragim Tajbakhsh
(Institut Pasteur – Paris - France)
Brendan Evano
(Institut Pasteur – Paris - France)

15:00 - 16:30 Lecture: James Wells

Engineering human gastrointestinal tissue (Cincinnati Children’s Hospital Medical
organoids from human pluripotent stem cells for Center – USA)
disease modeling and therapeutic discovery

COURSE 6 - STUDYING CELL FATE DECISIONS IN ESCS

17:00 – 18:00 Course 6 Alfonso Martinez Arias
Applying Stimuli (Universidad Pompeu Fabra - Barcelona -
Spain)
David Turner
(University of Liverpool - UK)

7 June 26 – July 7, 2023, Institut Pasteur, Paris France

 10th Advances in Stem Cell Biology
Thursday July 06 2023
09:00 - 10:30 Lecture Alfonso Martinez Arias
What do we learn by trying (and failing) to (Universidad Pompeu Fabra - Barcelona -
reproduce a mammalian embryo in a dish? Spain)
10:30 -10:45 Coffee break
10:45 - 12:15 Lecture Eve Gazave
Gradients of cell plasticity and proliferation (Institut Jacques Monod – Paris - France)
modulate the origin of blastema cells during
posterior regeration in the annelid Platynereis
dumerilii
12:15 - 14:15 Lunch Break
14:15 – 15:00 Visit of the Mouse Genetics Engineering Center Francina Langa Vives and CIGM team
(CIGM) (Institut Pasteur – Paris - France)
COURSE 9 - MOUSE GENETICS ENGINEERING CENTER
COURSE 6 - STUDYING CELL FATE DECISIONS IN ESCS
Course 8 Francina Langa Vives and CIGM team
Transgenesis Demo (Institut Pasteur – Paris - France)
15:00-16:30 (Group1)
16:30-18:00 (Group2)
15:00 - 18:00
Course 6 Alfonso Martinez Arias
Changing Medium (Universidad Pompeu Fabra - Barcelona -
15:00-16:30 (Group1) Spain)
16:30-18:00 (Group2) David Turner
(University of Liverpool - UK)

Friday July 07 2023

COURSE 6- STUDYING CELL FATE DECISIONS IN ESCS
09:00 - 10:30 Course 6 Alfonso Martinez Arias
Observation / Warm-up (Universidad Pompeu Fabra - Barcelona -
Spain)
David Turner
(University of Liverpool - UK)
10:30 -11:00 Coffee break and room change
11:00 - 12:30 Lecture : Arthur Lander
Regulation of proliferation in stem cell systems : (University of California – Irvine - USA)
the promise and perils of feedback control
12:30 -14:00 Lunch Break
COURSE 5 - MOUSE INTESTINAL ORGANOIDS: HOW TO MAKE MINIGUT
14:00 – 15:30 Course 5 Giulia Nigro
Observation (Institut Pasteur – Paris - France)
15:30 - 16:30 Lecture: Douglas Sipp
Economics and stem cell ethics (Center for Biosystems Dynamics
Research, RIKEN – Kobe - Japan)
16:30 –17:00 Coffee break and discussion
17:00 – 19:00 Wrap up & course closing Shahragim Tajbakhsh
(Institut Pasteur – Paris - France)
Laure Bally-Cuif
(Institut Pasteur – Paris - France)
20:00 Dinner with experts (Capitaine Fracasse (boat))

8 June 26 – July 7, 2023, Institut Pasteur, Paris France