H P L C / F O O D S A N D F L AV O R S

S E P A R A T I O N
Dietary Supplements/Plant Extracts
Vitamins (Fat Soluble)
on Brownlee Validated C18

Column: Brownlee Validated C18

N O T E
Peak List Concentration Conditions
(mg/mL) Mobile phase: acetonitrile:methanol (90:10, v/v)
1. solvent front NA Time (min) Flow (mL/min)
2. menadione (vitamin K3) 0.45 1.00 1.00
3. all-trans-retinol (vitamin A) 0.34 5.00 1.00
4. vitamin D3 0.4 5.01 2.00
5. unknown NA 50.0 2.00
6. α-tocopherol (vitamin E) 2.4 Temperature: 30 ˚C
7. α-tocopherol acetate 2.4 Detector: UV @ 280 nm
(vitamin E acetate)
8. unknown NA Sample
9. phylloquinone (vitamin K1) 0.84 Solvent: diethyl ether

Column
Column name: Brownlee Validated C18
Part number: N9303561
Dimensions: 250 x 4.6 mm
Particle size: 5 µm
Pore size: 100Å

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