PHAR2010

Spectroscopy Workshop 1 – Preparation Work Questions

1) In UV-visible spectroscopy:

a) Sketch the layout of a typical dual-beam spectrophotometer and describe the

two equations in visible spectroscopy that link absorbance of a substance with
its concentration?

b) From one of the above equations calculate the concentration of the original
sample in g/100ml from the information given below and suggest an
appropriate type of cell in which to measure the absorbance:

A sample of dye is diluted to 1/5th of its original concentration and measured

at 670 nm. The absorbance in a 1cm cell is 0.32 absorbance units and the
specific absorbance is 56 g/100ml/cm.

2) Explain which of the functional groups C=N or C=S will have the greatest
wavenumber for its bond stretch in the IR spectrum.

3) Which of the following C–H bonds has the highest energy vibration?

4) Match the following compounds with their IR spectrum. Give your reasoning and
evidence from the spectrum itself for each match.
 PHAR2010
Spectroscopy Workshop 1 – Preparation Work Questions
 PHAR2010
Spectroscopy Workshop 1 – Preparation Work Questions

5) Specify the integration and splitting pattern for the hydrogen atoms indicated in
the following molecules. Support your answers with reasoning.