BIOS2050 - Tutorial 3

For Question 1 to 4, indicate which of the following statements is TRUE (a) or FALSE (b).
1. Trans-placental transfer of maternal IgG antibodies against measles confers short-term immunity of fetus.
a) True
b) False

2. Attenuated vaccines are more likely to induce cell mediated immunity than killed vaccines are.
a) True
b) False

3. Both live attenuated vaccines and recombinant vector vaccines carry the same risk of reversion to
pathogenic form.
a) True
b) False

4. Immunohistochemistry rely on the use of enzyme-conjugated antibodies to bind the antigens found on
isolated cell grown in tissue culture suspension.
a) True
b) False

5. The usual sequence of events in an allergic reaction is as follows:

a) The allergen combines with IgG.
b) The allergen binds to IgE fixed to mast cells.
c) The allergen is processed by antigen-presenting cells and then binds to histamine receptors.
d) The allergen is processed by antigen-presenting cells and then binds to mast cells.

6. Which of the following remains the gold standard for asthma management?
a) epinephrine
b) antihistamines
c) corticosteroids
d) allergen desensitisation therapy

7. Systemic lupus erythematosus (SLE) is an autoimmune disease that causing symptoms such as painful and
swollen joints, rashes, chest pain and fever. SLE can be induced by ___________.
a) Type 1 hypersensitivity
b) Type 2 hypersensitivity
c) Type 3 hypersensitivity
d) Type 4 hypersensitivity

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 8. In Radioimmunoassay, the radioactive Iodine (125I) which binds to the exposed ______ residue on protein
antigen is being used.
a) Histidine
b) Cysteine
c) Tryptophan
d) Tyrosine

9. The administration of vaccines is not without hazard. Of the following, which is least likely to adversely affect
an immunocompromised host?
a) Attenuated vaccine
b) Inactivated vaccine
c) Subunit vaccine
d) Vector-recombinant vaccine

10. What is the advantage of polyclonal antibody?

a) Polyclonal antibody is with a single known antigen specificity.
b) Polyclonal antibody is useful for techniques such as agglutination.
c) Polyclonal antibody has the ability to form small antigen-antibody complex.
d) Polyclonal antibody from the same donor will have similar cross-reactivity with antigens.

11. Type 2 hypersensitivity reaction _____________.

a) is mediated by macrophages
b) is mediated by CD8+ T cells
c) requires immune complex formation
d) is antibody-mediated destruction of cells.

Question 11
For the following applications, would you select to use a polyclonal antibody preparation, a monoclonal antibody, or
more than one monoclonal antibody to detect your antigen? Explain your answer.

a) Immunoprecipitation

b) Western blotting

c) Solid-phase ELISA

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Question 12
For each of the case below, what do you think doctor will recommend to the mother toward the end of her first
pregnancy?

a) A mother has an Rh+ blood type and the father has an Rh- blood type.

b) A mother has an Rh- blood type and the father has an Rh+ blood type.