SODIUM POTASSIUM CHLORIDE

Method Lochhead & Purcell; Colorimetric

Albanese & Lein; Colorimetric Endpoint
Principle Bound sodium ions is released from
albumin by a solvent. The serum sodium
(bound, complexed, or ionized) is then
complexed by a dye forming a lavender
complex.
Endpoint
Bound potassium ions is released
from albumin by a solvent. The serum
potassium (bound, complexed, or
ionized) is then complexed by a dye
forming a blue complex.
Schoenfeld & Lewellen
Chloride ions in serum displaces
thiocyanate ions from the mercuric
thiocyanate forming mercuric chloride
which is only very slightly ionized. The
liberated thiocyanate ions reacts with
ferriions to form the orange-yellow
thiocyanate complex which is measured
at 480-492 nm. The color intensity is
proportional to the chloride
concentration.

Reagent Standard Sample Standard Sample Standard Sample

140 mmol/L 50 μl – 4.5 mmol/L 50 μl – Standard

50 μl –
100mmol/L
Sample – 50 μl Sample – 50 μl
Test/Sample – 50 μl
Sodium Base Potassium
1500 μl 1500 μl Chloride 1500 μl 1500 μl
Reagent Base 1500 μl 1500 μl
Na-K Color Reagent reagent
50 μl 50 μl
Developer Na-K Color
50 μl 50 μl Mix well and read %T/absorbance of
Developer
Read against water blank. Mix well and Read against water blank. Mix well each tube at 492 nm against water
read %T/absorbance of each tube at and read %T/absorbance of each tube blank. After one minute. Colorimetric
600nm against water blank. After one at 600nm against water blank. After endpoint, binochromatic
minute. Colorimetric endpoint, one minute. Colorimetric endpoint,
binochromatic binochromatic

Fully Automated
Parameters
Test Sodium Potassium Chloride
Method Endpoint Endpoint Endpoint
Standard 140 4.5 100
Unit Mmol/L Mmol/L Mmol/L
Temperature 25-30°C 25-30°C 25-30°C
Wavelength 550nm 600nm 492nm
Subwavelength 492nm 550nm 550nm
Blank Distilled H2O Distilled H2O Distilled H2O
Factor 630 11 118
Normal High 148 5.4 110
Normal Low 134 3.6 96
Delay time 10 secs 10 secs 10 secs
Test time 1 minute 1 minute 1 minute
Aspiration volume 400 UL 400 UL 400 UL

Semi-Automated
Parameters
Test Mode Standard Standard Standard
Select Filter 550 600 492
Select Diff. Filter NO NO NO
Value of Standard 140 4.5 100
Unit Code Mmol.L Mmol.L Mmol/L
Set Ranges NO NO NO
Read Blank Tube Distilled H2O Distilled H2O Distilled H2O

Normal Range
Serum: 134-148 mmol/L Serum: 3.6-5.4 mmol/L Serum: 96-110 mmol/L
CSF: 142-150 mmol/L CSF: 2.2-3.4 mmol/L CSF: 125-135 mmol/L
Urine: 27-287 mmol/L (24-hour urine Urine: 3.0-9.0 mmol/L (24-hour urine Urine: 170-255 mmol/L (24-hour urine
sample) sample) sample)