PRACTICE SCHOOL REPORT

ON
The Versatility and Power of
Polyherbal Formulation : A path to
innovative Treatment Approaches

Submitted for
Partial Fulfillment of the Requirements
For the Degree of
BACHELOR OF PHARMACY

Submitted by
Mr.Rishabh Pandey
Roll no.-2010920500043

Submitted to
Mrs.Gayatri Khosla
Associate professor

Galgotias College of Pharmacy

(Approved by PCI andAffiliated to Dr. A.P.J. Abdul Kalam Technical
University, Lucknow, U.P.)

Knowledge Park II, Greater Noida, U.P 201310

 GALGOTIAS COLLEGE OF PHARMACY
(Approved by PCI and Affiliated to Dr. A.P.J. Abdul Kalam Technical
University, Lucknow, U.P.)

STUDENT’S DECLARATION

I Rishabh Pandey Roll no. 2010920500043 hereby declare that the presented
report on The Versatility and Power of Polyherbal Formulation: A path
to innovative Treatment Approaches. under the practice school, BP 706
PS is a record of bonafide project work carried out and submitted by me after
completing 150 hr of practice school course work under the guidance of
Mrs.Gayatri Khosla
I also confirm that, the report is only prepared for my academic requirement
not for any other purpose.

Student name: Rishabh Pandey

Roll No : 2010920500043
Date :10/02/24
 GALGOTIAS COLLEGE OF PHARMACY
(Approved by PCI and Affiliated to Dr. A.P.J. Abdul Kalam Technical
University, Lucknow, U.P.)

ENDORSEMENT BYTHE SUPERVISOR

This is certify that the report entitled the study of The Versatility and Power
of Polyherbal Formulation A path to innovative Treatment
Approaches. is a bonafide work under the subject practice school, BP 706PS
done by Rishabh pandey Roll No.2010920500043 in a partial fulfillment of
the requirement of the degree of “Bachelor of Pharmacy” of A.K.T.U. Lucknow,
in the academic year 2023-24.
Date:

Signature
Mrs. Gayatri Khosla
Associate Professor
 GALGOTIAS COLLEGE OF PHARMACY
(Approved by PCI and Affiliated to Dr. A.P.J. Abdul Kalam Technical
University, Lucknow, U.P.)

ENDORSEMENT BYTHE DIRECTOR

This is certify that the report entitled the study of The Versatility and Power
of Polyherbal Formulation A path to innovative Treatment
Approaches. is a bonafide work under the subject practice school, BP 706PS
done by Rishabh pandey Roll No.2010920500043 . in a partial fulfillment of
the requirement of the degree of “Bachelor of Pharmacy” of A.K.T.U. Lucknow,
in the academic year 2023-24.

Date:

Signature
Dr. Vikram Sharma
Director
 ACKNOWLEDGEMENT
Presentation inspiration and motivation have always played a key role in the success
of any venture

I pay my deep sense of gratitude to Dr. Vikram Sharma, Director, Galgotias

College of Pharmacy, Greater Noida for his highest peak of encouragement and
for all the facilities that he provided for this project work.

I feel to acknowledge my indebtedness and deep sense of gratitude to my

Mrs.Gayatri Khosla Associate Professor whose valuable guidance and kind
supervision given to me throughout the course which s haped the present work as its
show.

I am immensely obliged to other staff (Lab technician, Librarian) and my friends for
their elevating inspiration, encouraging guidance and kind supervision in the
completion of my project.

Last, but not the least, my parents are also an important inspiration for me.
So with due regards, I express my gratitudes to them.

Student’s name-Rishabh Pandey

Roll no.2010920500043
Session-2023-24
 Table of Contents

Sr.No; Particulars Page No.

1 Introduction 1-2

2 Medicine from past 3-4

3 Formulation with multiple herbs 4-5

4 Factor affecting polyherbal formulation 6-7

5 Advantage of polyherbal formulation 8-9

6 Preparation of Polyherbal formulation and its evaluation 10-11

6 Limitaion 12-13

7 Conclusion 14-15

8 Reference 16-17
Introduction
The reliance of people on plants traces back to the beginning of the human
race. The essential requirements of life for the person are cover, clothing, food,
flavors and aromas, and not the least, drugs. Plants are a normal wellspring
of medication. Plants have molded the formation of reined conventional
medication structures among which are Ayurvedic, Unani, and Chinese are
normal . As indicated by the Kashaya Sangrahaya and Ayurveda Pharmacopeia
(1975), Maharasnadhi Quathar (MRQ) is a polyherbal detailing accepted to
have the potential for giving help [].The customary arrangement of
medication in India approvals several homegrown drugs [3].
Polyherbalism results in less expensive drugs by diminishing the length of
treatment or personal cost for mitigating antimicrobial prescriptions

Medicines from the Past

Since ancient times, people have utilized normal items, like plants,
creatures, microorganisms, and marine creatures, in meds to reduce and treat
infections. As indicated by fossil records, the human utilization of plants as
prescriptions might be followed back somewhere around 60,000 years. The
utilization of normal items as meds must have introduced an enormous test to
right on time people [5]. Plants will keep on being a source of new helpful
specialists however, because of their unregulated status, questionable viability,
and possible poisonousness, the gamble/ benefit proportion of herbals is
negative also, their utilization as prescriptions ought to be discouraged. The
acts of most cultivators and conventional healers are based on custom. They
have little
commonality with clinical writing and they may, justifiably, dislike the
interruption of ordinary medication into their space. In ongoing many years,
numerous distributions have stressed the significance of instructing customary
specialists to interface delicately with healers and patients from different
societies [6]. In any case, the worth of these frameworks is a lot more than
huge humanity or archeologic truth. Their worth is as a technique of therapeutic
specialists

Formulation with multiple herbs

First and foremost, PHFs are known to communicate high viability in countless
illnesses. As previously mentioned, the restorative impact of homegrown med is
applied because of the presence of various phytoconstituents and the impacts
are additionally potentiated when viable herbals are formed together in PHFs
[8]. Cultivators treat many circumstances like asthma, dermatitis,
premenstrual disorder, rheumatoid joint pain, headache, menopausal side
effects, persistent exhaustion, and touchy entrail condition, among others.
Natural arrangements are ideal for taken under the direction of a prepared
proficient [9]. It has additionally perceived a polyherbal treatment or spice
blend. The dynamic phytochemical constituents of individual plants are lacking
to accomplish the helpful restorative impacts. When polyherbal and herbs
mineral definitions consolidate the various spices in a careful proportion, it will
give an improved helpful impact and lessen the harmfulness. The dynamic
constituents utilized from the individual plants are insufficient to give alluring
pharmacological activity. There are confirmations that unrefined plant
removes frequently have more noteworthy intensity as opposed to disengaged
constituents. In customary medication, entire plants or combinations of plants are
utilized as opposed to disconnected compounds [10]. In the larger part of
customary frameworks, diabetes is better overseen by the spices blend
(Polyherbal) rather than a single spice given synergism and fewer side effects.
Diabetic injury cream ready by utilizing polyherbal detailing was viewed as
effective as well as protected in mending diabetic foot ulcers like the standard
silver sulphadiazine cream [11]. Some significant polyherbal detailing around
the world is referenced in table 1 and table 2.
Discussion
Nature has generally been remained a brilliant imprint to enhance the
extraordinary peculiarity of beneficial interaction. Ayurvedic solutions for
diabetes are generally blended definitions containing glucose bringing down
spices in mixes with an immunomodulator, detoxicates with the reasoning
behind such plans being given by present-day research. Polyherbal details
have plant-based pharmacological specialists who might apply synergistic,
potentiation, agonistic opposing activities by ideals of its assorted dynamic
standards inside themselves. These pharmacological standards cooperate
deeply to create the greatest helpful effectiveness with the least after effects.
Therapeutic plants existed even before a person showed up on the earth.
The advancement strategies of natural medications for overall use need to
be different structure of engineered drugs. The unrefined substances for
Ayurvedic medications were generally acquired from plant sources as rough
medications like dried homegrown powders or their concentrates or
combination of items or combination of items. Aside from these frameworks,
there has been a rich legacy of Ethno-plant utilization of spices by different
beautiful ancestral networks in the country. Henceforth, the current audit gives
the strong proof thought of a remedial methodology consolidating the
advantageous impacts of Polyherbal readiness in relationship with versatile
actual work for successful administration of diabetic inconveniences.

Polyherbal formulations have several advantages, including:

1-Effective: Polyherbal formulations are effective in treating many diseases,

including diabetes.
2-Safe: Polyherbal formulations are safe at high doses.
3-Fewer side effects: Polyherbal formulations have fewer side effects than single
herbal formulations.
4-Eco-friendly: Polyherbal formulations are eco-friendly.
5-Cheaper: Polyherbal formulations are cheaper.
6-Wound healing: Polyherbal formulations can promote wound healing by
stimulating physiological functions.
7-Nephroprotective effect: Polyherbal formulations can preserve renal damage due to their
antidiabetic and antioxidant actions.
Factor affecting polyherbal formulation:
1-Imperfect preparation Selection of the herbs should be done carefully based
on the required synergistic effect required to be produced.
2-Drug adulteration Adulteration is the practice of substituting the original crude
drug with a inferior property substance which sows less or spoiled therapeutic
effect
3-Faulty collection Some errors are made in herbal adulteration as a result of
the negligence displayed by collectors and suppliers of herbal products. It is
important to gather the appropriate sections of the genuine plant, while
refraining from collecting the less valuable parts.
4-Substiution with exhausted drugs Substitution with exhausted drugs: In this
type, the same drug is admixed but does not have any therapeutically active
constituents because these are already extracted out. This practice is more
common in volatile essential oils that contain drugs such as fennel, clove,
coriander, and caraway.

Advantages of polyherbal formulation:

While herbal medicines are widely used and considered safe, they are often used
in combination, sourced from various plants that may have their own species,
growing conditions, and biologically active components.
The main advantage of botanicals, compared to conventional single-component
drugs, lies in the presence of multiple active compounds. These compounds can
work synergistically, producing a potentiating effect that cannot be achieved by a
single compound alone.
Polyherbal formulations contain pharmacological agents derived from plants.
These agents can exert synergistic, potentiating, agonistic, and antagonistic
actions due to their associated diverse active principles. These principles work
together in a dynamic way, maximizing therapeutic efficacy while minimizing
side effects.
Synergism can occur through two mechanisms: pharmacodynamics and
pharmacokinetics. Pharmacokinetic synergism refers to the ability of one herb to
facilitate the absorption, distribution, metabolism, and elimination of other herbs.
(Ponrasu T, 2020)[11] On the other hand, pharmacodynamic synergism studies
the combined effect of active constituents with similar therapeutic activities that
target the same receptors or physiological systems. By acting on multiple targets
simultaneously, a combination of herbal remedies can effectively alleviate the
symptoms and complications caused by diseases.
This is particularly helpful considering that diseases often have multiple factors
and complications, leading to both visible and invisible symptoms. Polyherbalism
offers several benefits that are lacking in single herbal formulations. For instance,
it allows for better therapeutic effects with a lower dose of the herbal preparation,
thereby reducing the risk of side effects.Additionally, polyherbal formulations
provide improved convenience for patients by eliminating the need to take
multiple different herbal formulations simultaneously. This indirectly leads to
better compliance and overall therapeutic effects. Considering the multiple types
of molecules present in polyherbal formulations, they can effectively target
disease complications through different mechanisms, providing a comprehensive
therapy for the condition.

Material and Method:

Collection and authentication The medicinal plants used in the study were
purchased from the local market of Khari Baoli, New Delhi, India. Fresh and
shade dried roots of all selected plants have been authenticated by Botanical
Survey of India, the Northern regional .
Preparation of extract
The collected roots of the polyherbal plant were cleaned, dried under shade and
crushed by a mechanical grinder. The powdered roots of each selected plants
(1000 g) were poured in distilled water until the reach of complete exhaustion.
Successive extracts were separately filtered by using Whatman filter paper and
concentrated at an appropriate temperature (40 °C) on a rotary evaporator and
dried under freeze drier. The percentage yield was found between 20-25% (w/w)
respectively, and the final dried powder was stored in a closed container at a cool
place [15].
Characterization of powder
The biologically potent polyherbal extract powder with a stand for varied physical
properties and micromeritic properties. Extract powdered are heterogeneous
because it was composed of individual particles of different sizes and shapes
randomly interspersed with air spaces and becomes more complicated with
polyherbal [16, 17]. Measurements were carried out in triplicate for each
formulation and presented as the average±standard deviation (SD).
Angle of repose
The flow properties of thoroughly mixed all polyherbal extract powder in the
formulation were determined by calculating the angle of repose by the fixed
height method. A funnel with 10 mm in diameter of the bottom was fixed at the
height of 2 cm over the plain and smooth surface. About 10 gm of a thoroughly
mixed sample was slowly passed beside the wall of the funnel until the tip of the
pile formed and touches the bottom of the funnel. A rough circle drowned around
the pile base, and the radius of the powder cone was measured [18]. The angle of
repose was calculated by the average radius using the following formula given as
Evaluation of polyherbal dispersible tablet
Drug content uniformity test
From each formulation, 20 tablets were taken, weighed and thoroughly triturated.
The sufficient powder equivalent to 250 mg of the drug was accurately weighed
and diluted in 300 ml of 0.1N HCl for 10 min with vigorous shaking. Further, this
prepared mixture was diluted with 0.1N HCl to produce 400 ml and then filtered.
10 ml of this filtrate was diluted to 100 ml with distilled water, and the absorbance
was measured at an appropriate wavelength, i.e. Lower Hausner’s ratio-Better
flowability, Higher Hausner’s ratio Poor flowability [18, 19]. Preparation of
polyherbal dispersible tablet max 200-400 nm using UV spectrophotometer [21].
Disintegration time
From each formulation 6 polyherbal dispersible tablets were randomly selected
to determine the disintegration time. The acidic buffer (pH 1.2) used as
disintegration medium and temperature was maintained at 37±0.5 °C. The
average disintegration time of six tablets was noted down for calculation [22].
Dispersion time In vitro dispersion time of polyherbal dispersible tablet was
measured by dropping the tablet in a beaker containing 100 ml of normal water.
Two tablets from each formulation were randomly selected and in vitro dispersion
time was determined. A smooth dispersion was produced, which passes through
a sieve screen with a nominal mesh aperture of 710 µm [23, 24]. In vitro
dissolution study The dissolution testing of polyherbal medicines become
difficult to oversee authority requirements due to widely varying constituents.
The ingredients of polyherbal medicinal products often cover a mixture of
multiple herbal constituents; dissolution method development is much more
complex than for defined single constituent [25]. Stability
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Limitation:
Despite these advantages, there are limitations to polyherbal formulations. The
combination of various constituents may lead to chemical incompatibilities,
resulting in instability of the formulation. Therefore, careful consideration is
required when combining different plants with their constituents to ensure
optimal activity. In India, where most Ayurvedic polyherbal formulations are
manufactured and exported, the regulation of Ayurvedic herbal preparation
manufacturing is somewhat less stringent. Despite the existence of the Drugs and
Cosmetic Act, which aims to control the manufacture and quality control of herbal
formulations, toxicity studies and clinical trials are not mandatory for patent
applications or manufacturing licenses granted to Ayurvedic herbal product
manufacturers. polyherbal formulations provide numerous advantages over
single herb remedies. They offer the potential for synergistic effects, lower doses,
improved convenience, and a comprehensive approach to disease treatment.
However, caution must be exercised to ensure chemical compatibility and
stability. Although regulations in India may be more lenient, it is important to
uphold good clinical practice, toxicity studies, and clinical trials to ensure the
safety and efficacy

Conclusion
In spite of the fact that polyherbal detailing is generally utilized in many pieces
of the world, however, the scientific proof is as yet deficient. Numerous
homegrown treatments are yet under in-vivo assessment and have not been
assessed by clinical preliminaries. Besides, well-being assessments, for example,
toxicological investigations have not been performed. Here is a need of time
to assess polyherbal detailing utilizing scientific strategies like clinical
preliminary, conceivable bioactive mixtures, and systems of activity for the
future world.

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