BSA 3000_Operator Manual_ENG_V12.

00 | 1
Revision History

Version Date Modification

07.00 11/05/09 Initial release
11.00 27/06/11 Cover change
12.00 24/04/12 Design change and photo update

BSA 3000_Operator Manual_ENG_V12.00 | 3

Table of Contents

1. Description......................................................................................... 13
1.1. SPECIFICATIONS .............................................................................................................................. 13
1.2. FRONT VIEW ................................................................................................................................... 14
1.3. REAR VIEW ...................................................................................................................................... 14
1.4. INSTALLATION ................................................................................................................................. 15
1.4.1. OPERATING ENVIRONMENT............................................................................................................... 15
1.4.2. POWER REQUIREMENTS .................................................................................................................... 15

2. System menu ..................................................................................... 17

2.1. ANALYSIS ......................................................................................................................................... 17
2.1.1. SELECT TEST ....................................................................................................................................... 17
2.1.2. ANALYSIS ............................................................................................................................................ 17
2.2. SETTINGS ........................................................................................................................................ 18
2.2.1. DATE AND TIME ................................................................................................................................. 18
2.2.2. PRINTERS ........................................................................................................................................... 18
2.2.3. PROGRAM .......................................................................................................................................... 19
2.3. QUALITY CONTROL ......................................................................................................................... 22
2.3.1. QC GRAPHICS ..................................................................................................................................... 22
2.3.2. SETTINGS ........................................................................................................................................... 22
2.3.3. DELETE DATA ...................................................................................................................................... 22
2.3.4. PRINT ................................................................................................................................................. 22
2.4. DAILY REPORT ................................................................................................................................. 22
2.5. WORK LIST ...................................................................................................................................... 22
2.5.1. EDIT WORKLIST .................................................................................................................................. 22
2.5.2. PRINT WORKLIST ................................................................................................................................ 22
2.5.3. SAMPLE MEASUREMENT: .................................................................................................................. 23
2.6. RESULT REVIEW .............................................................................................................................. 23
2.7. TRANSFER ....................................................................................................................................... 23
2.8. SERVICE ........................................................................................................................................... 23
2.8.1. VOLUME CAL. ..................................................................................................................................... 23
2.8.2. FEED PAPER ........................................................................................................................................ 24
2.8.3. LIGHT SOURCE ................................................................................................................................... 24
2.8.4. ZERO DRIFT CHECK ............................................................................................................................. 24

3. Operation .......................................................................................... 25
3.1. CHECK BEFORE POWER-ON ............................................................................................................ 25
3.2. POWER-ON ..................................................................................................................................... 25
3.3. CALIBRATING THE ASPIRATION VOLUME........................................................................................ 25
3.4. CHECKING FILTER ............................................................................................................................ 25
3.5. SETTING TEST PARAMETERS ........................................................................................................... 25
3.6. MEASURE ........................................................................................................................................ 25
3.7. EXIT TEST......................................................................................................................................... 26
3.8. WASH FLOW CELL ........................................................................................................................... 26
3.9. SELECT OTHER TESTS ...................................................................................................................... 26
3.10. PRINT TEST RESULTS ....................................................................................................................... 26

4. Maintenance ..................................................................................... 27

BSA 3000_Operator Manual_ENG_V12.00 | 5

5. Troubleshooting ................................................................................. 29

Appendices-TSB ......................................................................................... 31
APPENDIX 1: SAFETY SYMBOLS................................................................................................................ 31
APPENDIX 2: REAGENT AND CONSUMMABLES LIST ............................................................................... 33
APPENDIX 3: ABREVIATIONS .................................................................................................................... 35

6 | BSA 3000_Operator Manual_ENG_V12.00

Statement

SFRI Sarl owns all rights to this unpublished work and intends to maintain this work as confidential.
SFRI Sarl may also seek to maintain this work as an unpublished copyright. This publication is to be
used solely for the purposes of reference, operation, maintenance, or repair of SFRI Sarl equipment.
No part of this can be disseminated for other purposes.

In the event of inadvertent or deliberate publication, SFRI Sarl intends to enforce its rights to this
work under copyright laws as a published work. Those having access to this work may not copy, use,
or disclose the information in this work unless expressly authorized by SFRI Sarl to do so.

All information contained in this publication is believed to be correct. SFRI Sarl shall not be liable for
errors contained herein nor for incidental or consequential damages in connection with the
furnishing, performance, or use of this material. This publication may refer to information and
protected by copyrights or patents and does not convey any license under the patent rights of SFRI
Sarl, nor the rights of others. SFRI Sarl does not assume any liability arising out of any infringements
of patents or other rights of third parties.

Content of this manual is subject to changes without prior notice.

Responsibility on the Manufacturer Party

SFRI Sarl is responsible for safety, reliability and performance of this equipment only in the condition
that:
• all installation, expansion, change, modification and repair of this equipment are conducted
by SFRI Sarl qualified personnel;
• applied electrical appliance is in compliance with relevant National Standards;
• the analyzer is operated under strict observance of this manual.

Warning
For continued safe use of this equipment, it is necessary that the listed instructions are followed.
However, instructions listed in this manual in no way supersede established medical practices
concerning patient care.
• This equipment is intended for use only by medical professionals in health care
institutions.
• To avoid electrical shock, you shall not open any cover by yourself. Service must be carried
out by qualified personnel.
• It is dangerous to expose electrical contact or applicant coupler to normal saline, other
liquid or conductive adhesive. Electrical contact and coupler such as cable connector,
power supply must be kept clean and dry. Once being polluted by liquid, they must be
thoroughly dried. If to further remove the pollution, please contact your biomedical
department or SFRI Sarl.

It is important for the hospital or organization that employs this equipment to carry out a reasonable
maintenance schedule. Neglect of this may result in machine breakdown or injury of human health.
Upon request, SFRI Sarl may provide, with compensation, necessary circuit diagrams, calibration
illustration list and other information to help qualified technician to maintain and repair some parts,
which SFRI Sarl may define as user serviceable.

BSA 3000_Operator Manual_ENG_V12.00 | 7

Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR
IMPLIED, INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR
PURPOSE.

Workmanship and Materials

SFRI Sarl guarantees new equipment other than accessories to be free from defects in workmanship
and materials for a period of one year from date of shipment under normal use and service. SFRI
Sarl's obligation under this warranty is limited to repairing, at SFRI Sarl’s option, any part which upon
SFRI Sarl's examination proves defective.

Exemptions
SFRI Sarl's obligation or liability under this warranty does not include any transportation or other
charges or liability for direct, indirect or consequential damages or delay resulting from the improper
use or application of the product or the substitution upon it of parts or accessories not approved by
SFRI Sarl or repaired by anyone other than a SFRI Sarl authorized representative.
This warranty shall not extend to any instrument which has been subjected to misuse, negligence or
accident; any instrument from which SFRI Sarl's original serial number tag or product identification
markings have been altered or removed, or any product of any other manufacturer.

Safety, Reliability and Performance

SFRI Sarl is not responsible for the effects on safety, reliability and performance of the BSA3000 if:
• Assembly operations, extensions, re-adjusts, modifications or repairs are carried out by
persons other than those authorized by SFRI Sarl.
• Personal unauthorized by SFRI Sarl repairs or modifies the instrument.

Warning
The BSA 3000 is an In Vitro Diagnostic instrument. It may indirectly bring risks to patients and
present a direct risk to operators and the environment during manipulation. To avoid these risks, be
sure to read the following instructions carefully.

This device uses AC 220V alternating courant. Do not open the rear cover before
switching off the power supply. If you need to open the rear cover of the device for
maintenance reasons, please contact our service personnel or professionals.

Patient samples are potentially contaminating agents. Manipulate with care using
appropriate protection equipment (glasses, gloves and laboratory coat) and be sure to
dispose of the waste in accordance with relevant regulations of your hospital.

Do not operate the device unless you have read this Operation Manual carefully

8 | BSA 3000_Operator Manual_ENG_V12.00

Company Contacts
Address: SFRI Sarl
Lieu-dit Berganton
33127 SAINT JEAN D’ILLAC
FRANCE
Phone: +33 (0) 556 688 050
Fax: +33 (0) 556 217 903
E-mail : support@sfri.com

BSA 3000_Operator Manual_ENG_V12.00 | 9

Foreword

The BSA-3000 is a semi-automatic high precision and high reliability chemistry analyzer with an open
reagent system. The analyzer uses optical, mechanical and computer technologies. It is an important
laboratory instrument running most routine clinical chemistry tests. Chemistry analyzers can be
divided into two categories – the fully automatic analyzer and the semi automatic analyzer. The fully
automatic analyzer completes most of the routine operations automatically, such as sample &
reagent pipe ting, mixing, heating, analyzing, calculating, printing and rinsing. It meets the demand of
hospitals with large sample volume and diverse test items.

The semi automatic analyzer automates certain operations (such as heating, analyzing, calculating
and printing), while reaction solution has to be prepared manually. This type of instrument is more
suitable to small laboratories and hospitals with smaller sample volume.

BSA 3000_Operator Manual_ENG_V12.00 | 11

1. Description

1.1. Specifications
Measuring Method
End point, Kinetic, two point Kinetic, Fixed time, Calibration
Absorbance, Turbidimetry. Linear
Regent blank, Sample blank Non linear up to 6 points
Monochromatic and bichromatic method Factor
Reaction curve displayed
Memory Storage
Light Source 3000 results per day available in memory at any
Quartz halogen lamp 12 V/ 20 W time and transferable via LIS
Stray light: <1.0% at 340 nm
Lamp saving feature largely prolongs lamp life Thermostatic Control
By means of Peltier elements
Optics 25°, 30°, 37°C,
Filter wheel can hold up to 8 filters Precision: ± 0.1°C
7 standard filters: 340 nm, 405 nm, 492 nm, 510
nm, 546 nm, 578 nm, and 630 nm Input/Output
1 free position for extra filter RS232 port for mono-directional LIS
Bandwidth <8 nm
Anti-vibration and anti-disturbance optical Printout
system ensures highly accurate and reliable Built-in thermal printer, 57.5 mm wide paper,
results recording width 48 mm

Photometric Range Display

Measuring range: 0.0000 to 3.0000 ABS 6” LCD touch screen
Resolution: 0.0001 ABS Mono-color display
Drift: 0.002 ABS Visual and audible alarm available

Flowcell Operating Environment

Stainless steel with quartz window Temperature 15°C – 30°C
Optical path: 10 mm Humidity 20% - 80% (max humidity)
Measuring volume: 32 µl
Programmable aspiration volume: 200 - 2000 µl Power Requirements
A.C. 110/220 V ±10%; 50 – 60 Hz
Parameter Settings
Method Reaction type Dimensions
Wavelength Absorbance limit 445(W) x 190(H) x 420(D) mm
Temperature Aspiration volume
Reagent blank y/n Standards Weight
Sample blank y/n Linearity check 9 kg
Delay time Unit for results
Measuring time Regulation
IVD 98/79
Quality Control
Comprehensive quality control program,
Levey-Jenning graphics included

BSA 3000_Operator Manual_ENG_V12.00 | 13

 1.2. Front View
Printer

Touch screen

Start
key
Sample probe

1.3. Rear View

Power
Power Grounding
Peristaltic Fan Switch
RS232 Plug Pole
Pump

BSA 3000_Operator Manual_ENG_V12.00 | 14

1.4. Installation
1.4.1. OPERATING ENVIRONMENT
1. The instrument should be installed on a stable and solid platform that is free of mechanical
vibration and away from vibration source.
2. The environment should be as free as possible from dust, corrosive gas, loud noises and
electrical interference.
3. Avoid placing the instrument in direct sun or in front of a source of heat or draft.
4. Ambient temperature: 15~30°C, humidity: 20~80%.

1.4.2. POWER REQUIREMENTS

1. The input voltage should be 220/110 VAC +/- 10%, 50/60Hz.
2. Power supply and grounding should be correctly connected.

Note: Verify that the input voltage is compatible with the instrument and the proper type of fuse is
installed.

BSA 3000_Operator Manual_ENG_V12.00 | 15

2. System menu

The main menu of the BSA-3000 includes eight sub-menus, as the figure below shows.

2.1. Analysis
2.1.1. SELECT TEST
The semi-automatic chemistry analyzer can conduct only one type of test at a time. The operation is:
touch ANALYSIS and then select the test to be conducted.

2.1.2. ANALYSIS
For the end point, results should be taken when the reaction is over. Add the sample or the standard
to the reaction tube, and then add the reagent to the tube. After incubation, take the colorimetric
results on the instrument.
For the kinetic or fixed time, the results should be taken within the steady rate range of the reaction.
Add sample to the tubes, then add reagents and take results immediately.
For the end point, there are two ways to obtain the result. One is to check the A-C curve, and the
other is to calculate with the following equation:

C = (Sample ABS－
－Reagent Blank ABS－
－Sample Blank ABS)×Factor

Standard concentration
Factor =
Standard ABS—Reagent blank ABS

The equation of the fixed time is almost same as that of the end point. The difference is the sample
ABS, reagent blank ABS, calibrator ABS should be changed to the ABS variation (△ ) within the
measuring time.
Calculation of the kinetic method is based on ABS variation rate (△ min),

min × Factor

VT x 1000
Factor
VE x d x ε

TV: total reaction volume, SV: sample volume, d: optical path, ε: Moore constant.

BSA 3000_Operator Manual_ENG_V12.00 | 17

2.2. Settings

2.2.1. DATE AND TIME

Select DATE AND TIME and set the date and time with the following screens:
The date format is: Year-Month-Day.
The time format is: Hour Minute Second.
For example, if the time is 18:35 on December 23, 2007, then the operation is:
Touch DATE AND TIME;
Touch 2, 0, 0, 7, YES;
Touch 1, 2, YES;
Touch 2, 3, YES;
Touch 1, 8, YES;
Touch 3, 5, YES.

2.2.2. PRINTERS
Select PRINTER to switch on or switch off the printer

18 | BSA 3000_Operator Manual_ENG_V12.00

 2.2.3. PROGRAM
Select PROGRAM to set the different analysis protocols in the instrument

2.2.3.1. NEW TEST

26 tests are pre-programmed and stored in the instrument. The parameters of these tests can be
changed by the user, while the test number and test name are fixed and cannot be changed or
deleted. Besides the pre-programmed tests, the user can define new tests as necessary. Up to 6
characters can be entered for the test name.

Touch NEW TEST and the following menu will be displayed:

New Test____
15 LDH 16 HBDH
17 CK 18 CK-MB
19 CO2 20 BUN
21 Cr 22 Ca
23 p 24 MG
25 UA 26 AMS
27 28

Select a new number and then the following menu will be displayed.
Test Name
1 ABC 2DEF
3 GHI 4JKL
5 MNO 6PQR
7STU 8VWX
9YZ 0
. NEXT
YES CLR

For example, assuming ABS is to be entered, the operation is: touch 1ABC twice, then “A” is
displayed. Touch NEXT; touch 1ABC three times, then “AB” is displayed. Touch NEXT, touch 7STU
twice, then “ABS” is displayed. Touch YES and return to the previous menu.

Warning
Changing the test name is prohibited if the result of this test is still present.

2.2.3.1.1. EDIT TEST

Different tests require different parameters. Test parameters include: method, wave length,
temperature, aspiration volume, reagent blank, delay time, measuring time, reaction type, ABS limit,
decimal, standard, factor, linear check, unit, measure range and normal range. The following
description takes glucose as the example.
To set each parameter click on it and the different possibilities will appear. Select then the correct as
for example for the method.

BSA 3000_Operator Manual_ENG_V12.00 | 19

 2.2.3.1.2. METHOD
The following methods can be selected: Absorbance, End Point, Fixed time, and Kinetic. Select End
Point for the glucose. The operation is: touch 09GLU, METHOD and select END POINT.

2.2.3.1.3. WAVELENGTH
Seven wavelengths (340nm, 405nm, 492nm, 510nm, 546nm, 578nm, 630nm) can be selected. For
glucose, the wavelength is 492nm.
The operation is: touch WAVELENGTH and select 492NM. Reselect WAVELENGTH 2 times a “2”
appears on the right of wavelength then:
If you want to work in Monochromatic mode select 492NM again
If you want to work in Bichromatic mode select the 2nd wavelength

2.2.3.1.4. TEMPERATURE
4 options available -- room temperature, 25℃, 30℃ and 37℃. Room temperature is selected for
GLU. The operation is: touch TEMPERATURE and select ROOM TEMP.

2.2.3.1.5. REAGENT BLANK

Yes --with reagent blank, No -- without reagent blank. No reagent blank for the glucose. The
operation is: touch REAGENT BLANK and select No.

2.2.3.1.6. DELAY TIME

For the Absorbance and End Point, the delay time is usually 1 second. For the fixed time and kinetic,
the delay time should be set according to the reagent instruction sheet. The operation is: touch
DELAY TIME then enter the required time.

2.2.3.1.7. MEASURING TIME

For the absorbency and end point, the measuring time is usually 1 second. For the fixed time and
kinetic, the time should be set according to the reagent instruction sheet. The operation is: touch
MEASURING TIME then enter the required time.

Note: The interval reading time for fixed time and kinetic method is 10 seconds.

2.2.3.1.8. REACTION TYPE:

Increase: the absorbance value increases during the reaction.
Decrease: the absorbance value decreases during the reaction.
This parameter does not apply to the glucose. Skip this item.

20 | BSA 3000_Operator Manual_ENG_V12.00

 2.2.3.1.9. ABS LIMIT
The range is 0.0000-3.0000A. For the increase reaction, the absorbance of the last reading point
should not be greater than the limit. Otherwise, the result is followed by a “*”. For the decrease
reaction, the absorbance of the last reading point should not be lower than the limit. Otherwise, the
result is followed by a “*”. This parameter does not apply to the Absorbance and End Point.

2.2.3.1.10. DECIMAL
0 for integer results, 1 for one decimal place, 2 for two decimal places, 3 for three decimal places.
Select 2 for GLU. The operation is: touch DECIMAL and select 2.

2.2.3.1.11. FACTOR
For the kinetic, the factor is entered according to the reagent instruction sheet. For the end point
and fixed time, the factor is calculated by measuring the standard. For the absorbance, the factor is
1. For the glucose, the factor is calculated by measuring the standard.

2.2.3.1.12. LINEAR CHECK

If the measuring result is non-linear, NL will be displayed. There is no linear check for the end point
or the absorbance.

2.2.3.1.13. UNIT
Seven result units can be selected: U/L, mmol/L, umol/L, mg/L, G/L, mg/dl and ABS. For the glucose
the unit is mmol/L. The operation is: touch UNIT and select MMOL/L .

2.2.3.1.14. ASPIRATION VOLUME

Set the volume of the reaction mixture to be aspirated into the flow cell. Too small aspiration volume
may cause high carryover. The recommended volume is 500ul~1000ul. For example, assuming 1000ul
is to be entered, the operation is: touch ASPIRATION VOL., enter 1 0 0 0, then touch YES.

2.2.3.1.15. MEASURE RANGE

“$”is displayed in case the result exceeds the range. For the glucose, the range is 0.00-2.22. The
operation is: touch MEASURE RANGE H enter 2 . 2 2 YES, then touch MEASURE RANGE L, enter 0
. 0 0 YES.

2.2.3.1.16. NORMAL RANGE

Reference range for normal results. “H” or “L” is automatically printed when the test result exceeds
the range. The operation is similar as previous section.

2.2.3.1.17. STANDARD POINT

Four calibration modes are available: 0, 1, multi-point linear and multi-point non-linear. “0” means
no standard and applies to the absorbance and the Kinetic. “1” means only one standard and applies
to the end point and the fixed time. Up to 6 standards can be defined with the multi-point linear
method or multi-point non-linear method. This applies to the end point only. Select 1 for the glucose.
The operation is: touch STD POINt and select 1.

2.2.3.1.18. STANDARD VALUE

Enter the standard concentration accordingly. No standard value entered for 0 standard point. Enter
one standard value for 1 standard point. Enter 2~6 standard values for multi-point settings.

Note: If less than 6 standards to be defined, then after entering all the standard values, set the value
of the next point to 0. For example, if 4 standards to be defined totally, then set the value of the 5th

BSA 3000_Operator Manual_ENG_V12.00 | 21

point to 0. In this way the system can decide the number of standards. If the standard value of the
glucose is 5.55, the operation is: touch STD 1, enter 5 . 5 5 YES.

2.3. Quality Control

QC graphics, settings, deleting data and printing data are included.
Select QC from the main menu:

It is possible to set 2 different QC file for each parameter.

2.3.1. QC GRAPHICS
Established from QC data automatically.

2.3.2. SETTINGS
Set mean value and SD value.

2.3.3. DELETE DATA

Select DELETE LAST MONTH and only the QC data of the last month will be deleted. Select DELETE
ALL DATA and all QC data of the past 31 days will be deleted.

2.3.4. PRINT
Touch PRINT to print out detailed QC information.

2.4. Daily report

Touch DAILY REPORT then all the test results of current date will be printed out by the sample ID.

2.5. Work list

2.5.1. EDIT WORKLIST
Touch WORKLIST to input the work list at the main menu. Select the sample ID and then select the
tests to be conducted. The selected items will be displayed in reverse video. Touch EXIT after
entering all the tests for this sample. Repeat this operation for all the samples.

2.5.2. PRINT WORKLIST

Check and confirm the work list. Touch PRINT BY ID then all the selected test items will be printed
out by the sample ID. Check and correct the errors, then proceed to next step.

22 | BSA 3000_Operator Manual_ENG_V12.00

Print a list by the test. Touch PRINT BY TEST then a list will be printed out by test items. This helps
the user to prepare the samples and reagents properly.

2.5.3. SAMPLE MEASUREMENT:

At the main menu, touch MEASURE and all the tests will be displayed. Each test to be conducted is
preceded by a “ ”. Select the test to enter the menu of the next level. After running zero,
standardization and quality control, touch SAMPLE to measure the samples. (When “ ” is displayed
inside ID: XXX, the sample ID will change automatically according to the work list, thus it cannot be
changed by the user) Measure the samples in turn. The measuring results will be saved to the system
automatically. When all the samples for the current test item are finished, “ ” will blink and
disappear, then the user can change the sample ID freely and measure the suspicious samples again.
After that, rinse the flow cell with distilled water and return to the main menu. Repeat this step for
the other tests. Proceed to next step when all the tests are finished.

2.6. Result review

There are two ways to review results, by test or by sample.
The operation is: select REVIEW BY TEST, select the test name, then all the results for this test will be
printed out or REVIEW BY ID, enter the sample’s ID number, then all the results for this sample will
be printed out.

2.7. Transfer
Select TRANSFER to transmit the sample result data and QC data to PC. When the transmission is
done, the system will return to the previous menu.

2.8. Service
Select SERVICE the following screen is displaying:

2.8.1. VOLUME CAL.

After a certain period, the aspirating force of the pump tube may change, which will cause the
discrepancy between the set aspiration volume and the actual aspiration volume. Therefore,
aspiration volume should be calibrated regularly, usually once per week.
Select ASP. VOL. ADJUST, then present 1 ml distilled water to the sample probe. Press the Start key
to active the aspiration. When the water is all aspirated into the instrument, release the Start key to
stop the aspiration, then the new calibration value is displayed. Usually this step is to be repeated

BSA 3000_Operator Manual_ENG_V12.00 | 23

several times until the difference between the new calibration value and current value is less than
10%.

2.8.2. FEED PAPER

The operation is: touch FEED PAPER and the printer will run a while to load the paper.

2.8.3. LIGHT SOURCE

To switch on or off the light select LIGHT STATUS, and select TURN ON or TURN OFF. The
corresponding image will be displayed.

2.8.4. ZERO DRIFT CHECK

Fill the flow cell with distilled water, continuously read the absorbance at a wavelength of 340 nm for
10 minutes. The difference between the maximum and minimum absorbance value should be no
greater than 0.006 ABS. The operation is: touch ZERO DRIFT, present the distilled water to sample
probe, then press the Start key. The zero drift check will start after the aspiration. The maximum,
minimum and current absorbance values (ABS) are all displayed on the screen. After 10 minutes, the
system will display the message “Zero drift check passed” or “Zero drift check failed”.

1.7.1. FILTER CHECK

Check the position and absorbance of each filter. For the new instrument, all the filters’ absorbance
should be less than 0.200, except for the free position (F8). The operation is: touch FILTER CHECK,
present the distilled water to the sample probe, and press the Start key. After the aspiration is done,
the filters will be checked and the results will be displayed on the screen.

24 | BSA 3000_Operator Manual_ENG_V12.00

3. Operation

3.1. Check before Power-On

The operating environment should be as free as possible from dust, corrosive gas, mechanical
vibrations, loud noises and electrical interference. Avoid placing the instrument in direct sun or in
front of a source of heat or draft. Power supply and grounding are properly connected. The input
voltage should be compatible with the instrument. Ambient temperature and humidity is within the
related requirements.

3.2. Power-On
Turn on the power. After initialization, the system will enter the main menu automatically.

Warning
Proceed to the following operations only after 10~15 minutes warm-up.

3.3. Calibrating the Aspiration Volume

Usually the aspiration volume is calibrated once a week. See Section 5.8.1.

3.4. Checking Filter

See Section 5.8.5.

3.5. Setting Test Parameters

Different tests require different parameters. Set the parameters as instructed by Section 5.2.3 “Edit
Test ”. The parameters are saved automatically.

3.6. Measure
The measuring procedure is explained as follows (take the glucose as the example):
From the main menu select ANALYSIS
Select the test. Click for example on 09GLU

Before measuring, check the parameters that are displayed on the top of the screen, and make sure
they are correct.
Then:
• Select ZERO and present the distilled water to the sample probe. Press the START key to
aspirate the distilled water. After zeroing, “A: 0.000” will be displayed on the screen.

Note: If the test parameter for “Reagent Blank” is “No”, the user should execute this step with
reagent instead of distilled water.

BSA 3000_Operator Manual_ENG_V12.00 | 25

 • Select RB and present the reagent to the sample probe. Press the Start key to aspirate the
reagent. After reading the absorbance of the reagent, the value will be displayed on the
screen.

Note: If the test parameter for “Reagent Blank” is “No”, RB will not be displayed.

• Select STANDARD and present the standard-reagent mixture to the sample probe. Press the
START key to aspirate the mixture. The absorbance reading of the mixture and the factor are
displayed. The factor is calculated and saved into the instrument automatically.
• Select QC and present the control to the sample probe. Press the Start key to aspirate the
control. The absorbance reading and measuring result of the control are displayed. The
measuring result is saved into the QC file.
• Select SAMPLE and present the sample to the sample probe. Press the START key to aspirate
the sample. The absorbance reading and measuring result of the sample are displayed. The
result is also printed out automatically. Repeat this step to measure the samples in turn.
• If you want to change the sample ID press NEXT ID or ID XXX to change the sample ID.

Warning
Make sure the sample has the same ID for different tests.

• Rinse: the flow cell should be rinsed after each type of test. First rinse with distilled water,
then rinse with the recommended detergent, and finally rinse with distilled water again. The
rinsing volume should be no less than 2 ml. Select RINSE, press the Start key, and rinse the
flow cell with distilled water – detergent – distilled water.

3.7. Exit Test

Touch EXIT to exit the menu.

3.8. Wash Flow Cell

See Section 6.6.

3.9. Select Other Tests

Repeat step 6.6 - 6.8.

3.10. Print Test Results

When all the tests are done, select DAILY REPORT to print all the results by sample ID.

Warning
Before shutdown, make sure to wash the flow cell thoroughly and fill it with distilled water.

26 | BSA 3000_Operator Manual_ENG_V12.00

4. Maintenance

1. The instrument must be operated under the environmental requirements.

2. After turning on the power or the lamp, wait 15~20 minutes before starting the test. After
turning off the lamp, wait about 10 minutes for the lamp to cool down before turning it on again.

3. Make sure the measurement is free of bubbles.

4. Wash the flow cell after finishing each kind of test.

5. Check the aspiration volume regularly. If necessary, replace the aged tube.

6. Fill the flow cell with distilled water before shutdown.

7. Handle and dispose of the waste according to acceptable laboratory, local state and national
standards.

BSA 3000_Operator Manual_ENG_V12.00 | 27

5. Troubleshooting
PROBLEMS CAUSES
Black Screen after 1. Poor connection of the power
Power-On cable.
2. Broken fuse.
Abnormal Aspiration or 1. Aged peristaltic pump tube.
no Aspiration 2. Tubing system blocked.
3. Tubing system leaking or
loose.
4. Defective peristaltic pump
motor.
No Aspiration When Start Calibrated value for aspiration
Key is Pressed volume is lost.
Abnormal Display of A-C Improper value for standard
Curve point and concentration.
Absorbance of the Filter 1. Bubbles in flow cell.
Is too High 2. Deviated optical parts.
3. Aged lamp.
4. Damaged filter.
Poor Repeatability of 1. Dirty flow cell subject to
Test Results remaining bubbles.
2. Tube blocked.
3. High carryover due to
inaccurate aspiration volume.
4. Aged lamp.
5. Poor internal ventilation or
ambient temperature too high.
6. Polluted reagent.
Results are too High or 1. Inaccurate standard volume.
too Low 2. Poor standard.wrong factor.
3. Polluted reagent.
4. Wrong temperature setting
for kinetic method.
5. Wrong sample-to-reagent
ratio.
ACTIONS
1. Replace peristaltic pump tube.
2. Eliminate blockage.
3. Check the tubing system, if
necessary, change the aged tube.
4. Replace peristaltic pump motor.
5. Contact the manufacturer.
1. Shut down and re-start the
instrument.
2. Enter the SETUP > VOLUME CAL
menu to re-calibrate the aspiration
volume.
Set the correct parameters and
re-create the curve.
1. Wash flow cell with proper
detergent.
2. Adjust the optical components to
correct position.
3. Replace the lamp.
4. Replace the filter.
1. Wash flow cell with proper
detergent and keep bubbles away.
2. Replace the tube.
3. Re-calibrate aspiration volume or
replace the peristaltic pump tube.
4. Replace the lamp.
5. Operate the instrument under
proper environment.
6. Use qualified reagent.
1. Add standard of correct volume.
2. Use qualified standard.
3. Correct the factor.
4. Use qualified reagent.
5. Correct the temperature setting.
6. Prepare sample and reagent as
instructed.
1. Replace peristaltic pump tube.
2. Eliminate blockage.
3. Check the tubing system, if
necessary, change the aged tube.
4. Replace peristaltic pump motor.
5. Contact the manufacturer.
BSA 3000_Operator Manual_ENG_V12.00 | 29
Appendices-TSB

Appendix 1: Safety Symbols

SYMBOL WARNING CONDITION ACTION
Biohazard: Consider all materials Wear standard laboratory attire,
(specimens, reagents, controls, glove and follow safe laboratory
calibrators, or components that contain procedures when handling any
or have contacted human blood) as being material in the laboratory.
potentially infectious.
Electrical Shock Hazard: The electrical Before continuing, unplug the BSA
warning icon alerts users to the possibility analyzer from the electrical outlet.
of electrical shock in the described
activity or at the posted location.
Probe Hazard: The probe may contain Avoid any unnecessary contact with
biohazard materials, including controls the probe and probe area.
and calibrators.
NOTE: Indicates to the operator
information of importance in the
procedure to be performed. This heading
is also used to indicate specific sample
handling techniques that are helpful in
operating the instrument.
CAUTION: Indicate to the operator
information of importance that could
result in damage to the instrument or
affect the test due to improper operation
if these instructions are not followed.
WARNING: Indicate to the operator
information regarding potential hazards
that may cause personal harm to the
operator if these instructions are not
followed.

BSA 3000_Operator Manual_ENG_V12.00 | 31

Appendix 2: Reagent and consummables list
REAGENT REFERENCE VOLUME

CONSUMMABLE REFERENCE QUANTITY

BSA 3000_Operator Manual_ENG_V12.00 | 33

Appendix 3: Abreviations
NO. ABBREVIATION FULL NAME
01 ALT Alanine aminotransferase
02 AST Aspartate aminotransferase
03 ALP Alkaline phosphatase
04 GGT Gamma Glutamyl transferease
05 T.BILI Total bilirubin
06 D.BILI Direct bilirubin
07 TP Total Protein
08 ALB Albumin
09 GLU Glucose
10 CHOL Cholesterol
11 TRIG Triglyceride
12 HDL-CH High density lipoprotein-cholesterol
13 APOA1 Apolipoprotein A1
14 APOB Apolipoprotein B
15 LDH Lactic dehydrogenase
16 HBDH α-hydroxybutyrate
17 CK Creatine kinase
18 CK-MB MB isoenzyme of CK
19 CO2 Carbon dioxide
20 BUN Blood urine nitrogen
21 Cr Creatinine
2+
22 Ca Calcium
23 P Phosphorus
24 Mg2+ Magnesium
25 UA Urine acid
26 AMS Amylase
27 K Kalium
28 Na Natrium
29 Cl Chlorine

BSA 3000_Operator Manual_ENG_V12.00 | 35