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THC Acetate
Posted by pharmerjoeoakes
Date: March 02, 2012
in: Safety, Experiment
(77) Comments

I was in my first semester of Organic chemistry and had recently synthesized aspirin
when Graywolf brought THC-O-Acetate to my attention.Our research into Acetate
started and we were all very impressed by the outcome of our first reaction.

We have since followed this procedure many times with varied efficiency This
variability was caused by and directly proportional to the amount of essential oil in the
reaction.The more oil the less efficient the reaction. The maximum amount of oil I use is
20g.

When making aspirin, salicylic acid is the raw material for the reaction, Acetic Anhydride
is the conjugate and Sulphuric acid (H2SO4) *is the catalyst.

The similarities between Salicylic acid and THCA are the carboxylic acid groups that
direct the acetic anhydride to form an ester with the phenolic hydroxyl (OH). In fresh
plant material of cannabis, most cannabinoids are present as their carboxylic acid form,
known as acidic cannabinoids [Shoyama, 1975].

Since carboxylic acid and hydroxyl groups are polar and hydrophilic, small amounts of
THCA are soluble in water. They can be precipitated at a higher PH and made insoluble
at a low PH which can be used to our advantage. See our high purity extraction page for
more details.

When THCA is converted to THC-O- Acetate, the polar C-OH becomes C-O-CH2C=O-
CH3. The carboxylic acid group is hydrolyzed (forms water) by the heating with the
 H2SO4 which reacts with excess Anhydride to produce acetic acid which reacts with
regular THC at the hydroxyl group to form more THC-O-Acetate.

Simpler than it sounds, this is a highly efficient reaction, when *catalytic volumes of
H2SO4 are added and a Molar excess of acetic anhydride is present.

NOTE THIS DISCLAIMER:

Do not attempt this procedure if you are not qualified or without the proper
Chemical fume hood and proper personal protection equipment.

Sulfuric acid is highly corrosive, and Acetic anhydride is not only highly
corrosive, but as flammable and explosive as gasoline. Not to mention Hexane is
highly flammable and explosive! You should read, understand, and have ready a
Material Safety Data Sheet (MSDS) for all chemicals you have.

We neither recommend this procedure or accept any responsibility if you elect to

try it yourself.

Materials needed:

 Face shield
 Chemical goggles
 Respirator with acid cartridge
 Chemical apron
 Elbow length gloves
 Fume hood
 Heated Stirrer
 Flat Bottom boiling Flask
 Alhin Reflux condenser
 5 gal bucket of ice water
 Aquarium water pump
 Pasteur pipette's
 Beakers of various sizes
 Graduated cylinders
 Coffee filter and funnel
 Glass separatory funnel
 Chemicals

 Dry Ice in a foam cooler

 ½ gal 95% Ethyl Alcohol
 ½ gal Denatured alcohol
 Hexane HPLC grade
 98% Sulphuric acid
 98% Acetic anhydride (controlled substance)
 2 gal distilled water
 200g Kosher salt
 Baking soda
Basic procedure:

 Dry your extract in an oven at 220ºF until thoroughly dry and free of any alcohols.
 Dissolve your extract into an alkane solvent such as hexane (HPLC grade)
 In a flat bottom boiling flask combine extract in a 1:2.5 w/v ratio of oil to acetic
anhydride. (1g + 2.5mL).
 In an ice and water bath; add 2.5 drops/g oil of 98% H2SO4 slowly down the side of the
glass swirling gently after every couple of drops.
NOTE: This is where things can hit the fan! You should have proper PPE including a face
shield, chemical resistant apron, and elbow length chemical gloves. This again should be
done in a certified fume hood by qualified chemists. If you try this and it goes wrong, it’s not
our responsibility.

 Once the solution has cooled place a stir bar in carefully to avoid splashes.
 Put on a stir plate and begin stirring vigorously
 Place a reflux condenser onto the top
 Begin heating until the vapor is being condensed at the second bulb. This takes a bit of
adjusting to get to the perfect plate temperature versus condenser water flow.
 Start a timer for 60 min for up to 10g and add 60 min for up to an additional 10g of oil.
 Turn off the hot plate but continue to stir
 When cooled slightly add 1mL (/g oil) of distilled water drop wise against the glass.
 Vapors containing acetic acid will escape from the top. This is normal. DO NOT INHALE
VAPORS!
 Pour liquid into a separatory funnel, wash flask clean with hexane and pour into funnel.
Wash flask of anything left with 95% non-denatured alcohol pour into funnel.
 Prepare 2 gal of distilled water with 100g kosher salt dissolved in each.
 Once the water can be distinguished from the hexane begin draining the water.
 When you are close to the emulsion layer, stop and add salt water until the funnel is
almost full.
 Cap and mix by inverting several times, uncap or the pressure will shoot the lid off.
 You should have a lower water layer an emulsion layer and an upper organic layer.
 Continue washing with salt water until both gallons are empty.
 Drain the organic layer into a clean beaker and place into an oil bath @ 250F until dry.
 Test a small drop of acetate on a watch glass with some baking soda dissolved in
water. If it fizzes, re-suspend acetate in hexane and wash with baking soda and water
until it stops bubbling. Re-separate water from organic in funnel.
 Dry at 250ºF
 Re-dissolve in 95% Ethanol and place beaker into a shallow, denatured alcohol and dry
ice bath for at least 5 min.
 Pour liquid through a coffee filter
 Discard filter and dry the acetate by boiling off the alcohol.
 Wash and dry 2 more times with ethanol
 Enjoy
Attached Photo's
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 About The Author

pharmerjoeoakes
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77 Comments

1.
 Naveron
July 15, 2019 at 10:43 PM

Hi there, SkunkPharm. I want to preface this by saying that I am an inorganic chemist,

but I want to try my hand at this procedure, but certain things about it bother me. My
primary concern after doing some research is that THC-O-Acetate is said to be water
soluble. Is this the case? If it is, am I right in assuming that the saline solution must be
absolutely saturated? Secondarily, your setup in figure 1 is a little confusing. The way I
understand it is that you have cold water in the bucket with an aquarium pump moving
water from the bucket through the reflux condenser and back to the bucket. Is this
correct? Third, I wanted to ask some questions about the oil requirements. First of all I
am fully aware that the addition of sulfuric acid to the oil will result in CBD being
converted to various forms of THC, some of which are not the delta-9 variant. My first
question is are any of these other variants detrimental to health/product? Second, how
pure must my initial oil be, and what are your tips for increasing purity using ethanol as
a primary solvent? do I need to do a secondary extraction with an alkane like hexane to
ensure that there are no water soluble impurities like chlorophyll present. Fourth, what
are ways this product can be used? Is it able to be used just like a normal THC oil in an
oil rig, or being baked into edibles? also Is it stable enough to be stored under normal
atmospheric conditions or are there special requirements for storing this chemical? I
would really appreciate your input. Given that this experiment has the potential to be
very hazardous, I want to make absolutely certain that I know what I am doing every
step of the way.

Reply

2.

Sparky75
June 12, 2019 at 8:47 PM

THC O-Acetate RSO If I follow the RSO recipe from extraction to distillation and finish
with my oil. Can I let the oil cool and then add acetic anhydride to the oil and distill the
acetate. I am thinking this should change the THC to THC O-Acetate. Then wash the
product with chemicals and a separatory funnel. My goal is to take THC (oil) and turn it
into THC O-Acetate (WATER SOLUBLE) so I can administer as a suppository. The
THC O-acetate is water soluble and will pass through the membrane of the sphincter,
where the THC (oil) will not pass through the membrane wall as well because it is a fat.
Thanks for the help.

Reply
3.

Rexkicker
April 13, 2018 at 4:40 PM

I was under the impression that acetic anhydride was used to avoid an equilibrium
reaction.

Reply

4.

Brian
February 16, 2018 at 9:04 AM

Most esterifications I’ve seen utilize an alcohol and carboxylic acid with H2SO4 as a
catalyst. Would this reaction work with glacial acetic acid (carbocylic acid) and
decarvoxylated thc (alcohol)? The glacial acetic acid is much safer to handle than the
anhydride, but likely less reactive. Let me know what you think!

Reply

5.

Matt
June 08, 2017 at 4:36 PM

Could you use an already decarboxylated extract to start with?

Reply

6.
 Matt
March 09, 2017 at 1:51 PM

You rekon 97% acetic anhydride would be suffecient for this reaction?

Reply

1.

Skunk Pharm Research,LLC

March 10, 2017 at 4:53 AM

Yes. GW

Reply

7.

Eric Day
October 04, 2016 at 10:34 PM

I'm a huge fan of all you do here - this is the best cannabis site anywhere with priceless
information for anyone wanting to make friends with this magic plant. Thank you all from
the bottom of my heart! Concerning the value of the acidic cannabinoids found in fresh
flowers and leaf, it was interesting to read the author's information. I've benefitted from
this form of the plant for the last 4 years and my experience with this might be of interest
to others. I started using leaf in my daily smoothie and it was good, but since the buds
are where the action is I started using frozen fresh buds instead. I never juiced any of it
since the trichs are sticky and not water soluble so would likely stick to the juicer and/or
get discarded with the green "waste" from the juicer. The effects were easily 10X what
they were using only leaf, and with typical commercial strains (high THC, low CBD)
these smoothies gave me a nice energy lift along with a mood lift as well - all without
any feeling of getting high. After a few months using them every day in a smoothie (this
first round was all high THC varieties) I suddenly realized when I bend/knelt down to
tend my plants, I was able to do this without pain. I'm old....so had made friends with my
beginning arthritis as a cost of getting old, along with typical prostate issues. Once I
noticed this lessening of arthritis pain (and greatly increased mobility in all my joints) I
also realized my lifelong depression had vanished as well. No more St. John's Wort -
 didn't need it. My prostate issues cleared up, my skin heals noticeably faster and overall
I feel 10 years younger. I've been using fresh/frozen buds in smoothies since 2012, and
at 62 can get up off the floor without using my hands (hadn't been able to do that for
many years) and no longer feel arthritic or depressed. Prostate issues have cleared up
as well. For the first couple of years I used it daily (maybe 5-7gm flowers/day), and
since then I've cut back to a couple-few times a week. I was baffled at the healing
aspect of fresh bud on my arthritis until I read that our cartilage has CB2 receptors, as
do many/most of our internal organs. Mystery solved! As exciting as true scientific
research on cannabis is these days, I feel if we wait for all the studies to be done we'll
lose precious time. The acidic cannabinoids have been labeled "bio-inactive" by many,
but if you suffer from any of the above ailments I urge you to try fresh/frozen cannabis
buds in your daily smoothies for a while and see what they do for you. And a plus -
harvest/freeze 'em and if you change your mind, you can still dry/smoke/extract them
without much if any loss of potency from what I've seen. They're darker but no less
effective. If you've gotten this far - thank you for your time, and I hope you have as
much success as I have eating fresh cannabis. My website has nothing to sell at all and
has a bit more info on this subject.

Reply

8.

joey
September 25, 2016 at 7:54 PM

what is the average yield percentage on this method? lets say i have a starting material
of 20g @ 80% potency after dewaxing and decabing. thanks!!

Reply

1.

carma
May 04, 2017 at 2:51 PM

isnt that oil the same thing as rick simpson oil???? which is made by
first decarbing the bud material (bm) then soaking the bm in 99%
isopropyl alcohol and stirring then boiling off the alcohol over a low
heat source.... then the oil that is left looks just like the oil u show up
 there... & it definitly cures cancer..... so whats the difference??? is that
more of a pure product? is that why someone would go to all that
trouble???

Reply

9.

TF
July 21, 2016 at 7:19 PM

In simple English can someone explain how to make this at home or where to buy such
thca tinctures or products? Can you simply grind up marijuana leaves and eat them to
consume tcha?

Reply

1.

Skunk Pharm Research,LLC

July 28, 2016 at 6:25 AM

Buy an homogenizer. Mix dry sieve or bubble hash with refined

coconut oil, and homogenize with homogenizer. Filter oil from solids.
Easier using a vacuum assisted filtration system, as pictured in the
article. I've never shopped for THCa tincture, but would start here in
Oregon, by calling the dispensaries. We used to make brownies by
adding ground plant material, but they tasted greeeeeeeeeeeeeeeeen,
and some folks digestive tracks took umbrage. They were of course
also not THCa. Extracting with a low polarity oil leaves the green
chlorophyll behind, so sidesteps that issue, and if done cold, retains
the THC in its acidic THCa form.
https://skunkpharmresearch.com/extracting-with-oils-and-fats/ GW

Reply

10.
 Joe
June 12, 2016 at 3:22 PM

Hello, Love your site, my hat's off to you! Can you suggest how to make a strong THC
tincture, for sublingual use..perhaps with VG or pg - without using alcohol, dry ice or
fancy lab equipment, Thanks!

Reply

11.

Thc Drops For Vapor Cigarettes – Real Cigarettes

May 09, 2016 at 9:03 PM

[…] THC Acetate | Skunk Pharm Research LLC – So with all the hassle and risk, was
the end result worth it? THC O acetate is legal in the USA as it is unscheduled Last time
I checked, which is just about 10 … […]

Reply

12.

Eric R.
March 31, 2016 at 12:30 PM

So with all the hassle and risk, was the end result worth it? THC O acetate is legal in the
USA as it is unscheduled Last time I checked, which is just about 10 minutes ago...

Reply

1.

myname1schris@yahoo.com
January 12, 2017 at 4:46 AM
 THC is most certainly illegal at the federal level, and is subject to the
Federal Analog act, which constitutes any analogous or drugs of
similar psychoactive effects-- the penalties, which are that of the drug it
is analogous too. It is an incredibly vague bill, however it covers a
whole swath of chemicals because of it.

Reply

13.

Troy Wahl
March 18, 2016 at 10:21 AM

If you want the acidic cannabinoids: take a crude extract mix throughly with a
water/alcohol solution containing sodium bicarbonate (baking soda) at pH ~9, then add
water and oil (vegetable oil should work fine). Mix throughly and let the layers separate,
the salts of the acids should be in the water layer (on the bottom). You will have to play
around with this to get optimum results. The water/alcohol can then be removed by
evaporation, preferably via a rotary evaporator at high vacuum (use a diaphragm pump
for this instead of oil pump or you'll risk destroying your pump to water). As sodium salts
the acidic cannabinoids will not decarboxylate, however they will still oxidize so they
should be stored under nitrogen or used quickly.

Reply

14.

Steve
March 07, 2016 at 6:30 AM

Analysis for the THCA solution: http://www.catscientific.com/wp-content/uploads/THCA-

Analysis-8-5-15.pdf Analysis for the CBDA solution: http://www.catscientific.com/wp-
content/uploads/CBDA-Solution-Analysis.pdf

Reply

15.
 Bizzle
February 10, 2016 at 9:36 PM

I can't see the results, maybe it's because I'm on mobile? Is it an image?

Reply

1.

cone conington
March 07, 2016 at 4:46 AM

Nope, I think the results were missed out.

Reply

16.

music hot
January 12, 2016 at 5:28 PM

After this process do all of your oil runs come out basically the same? In strength affect
and flavor does it still vary strain to strain? How many milligrams of acetate would you
say is an appropriate dose? Would you recommend against making oral capsules? I am
looking for a way to take a controlled dose that is the same every time with out having to
vaporize the oil. My thoughts were to maybe mix the acetate oil with some food oil and
put it in a capsule but I read you saying there are down sides to ingesting acetate. As
you seem to be very experienced in this I would ask your recommendation on how I
should administer my oil. I am also wondering how many doses I would be able to get
out of a gram of oil. Thanks for your time

Reply

17.
 Russell
December 21, 2015 at 12:46 PM

what fun to see folks doing organic synthesis in the shed. this was a fun substance to
make in the basement lab back in the 70's when we did not have the high yielding
strains of today. After a prosperous med chem career with big pharma I may look at all
this natural product isolation and derivatization again. Do you have a TLC system to
monitor the reaction and products? and ever try a spray reagent like FeCl3 that reacts
with phenols to check out the reaction? So what I think I would like to try is the synthesis
of the acetate of the THCA for a direct aspirin adduct. It will require some finness due to
the molecule being so prone to decarboxylation. An old man likes to feel no pain.

Reply

18.

Tips Kesehatan
December 21, 2015 at 7:17 AM

http://tipskesehatan.website

Reply

1.

Old Hippie
December 23, 2015 at 12:56 AM

It's spam.

Reply

19.

Tips Kesehatan
 December 21, 2015 at 7:17 AM

i like

Reply

20.

Gratis Mp3 Sepuasnya

December 21, 2015 at 7:16 AM

How to turn it into a powder form

Reply

21.

DANU
December 20, 2015 at 7:26 AM

very nice info , for other article search here http://ringmusic.co

Reply

22.

Hugh
September 27, 2015 at 8:06 AM

Very close to (or maybe is) a THCA-Ester, something that the Feds fear (potency, easy
conversion to effective tablet form). The FDA even authored a paper on more or less
this process, available on Medline. For the legal questions, the Feds have a new
criminal statute that looks at intent rather than a specifically listed compound. Nasty
stuff, that! The safety here is medical necessity and compassionate care, but they might
forget that with an ester compound. Really interesting work. Cheers -Hugh
 Reply

23.

Brian Carroll
April 28, 2015 at 5:32 PM

I love this stuff......& since currently I have access to 92% pure THC sans terpins I
believe this stuff will be quite popular in the next 5 years. Its not even specifically
scheduled on a federal or state level Outlaw life...... For life ;)

Reply

1.

King of Hemp
December 07, 2015 at 7:20 AM

All of this is definitely illegal on the federal level. I do not mean to rain
upon your parades. Cannabinoid Freedom Fighters certainly are my
people. They are welcome in my castle any time. If you look hard you
will see that I am correct. Even Cannabidiol is a controlled substance.
Some know this,Others do not. the problem is that the federal and
even state laws are so technical in their language that only those gifted
with the ability of scrutinizing word meanings and legal definitions can
really say so with any accuracy. I have made a point to study the
language of the federal laws. This might seem subjective, but I assure
you that language is everything and that even with no legal definition to
be found for a specific word, one is still able to define legal relevance
from even vague and nonspecific legal wording. I do not want to give
any examples as they would only fuel something I do not want to fuel.
Just know a good Prosecutor will find plenty of ammunition if he looks.
He will likely need a chemist. I only know these things because I
eat,sleep and shit in the world of chemistry. There is a difference in
being able to read chemistry and do chemistry. Not everything is
simple read and do. Do not let the internet fool you. The reason why
you have go through legal channels for all this is not because Uncle
Sam is cool. It has to do with safety. I realize this whole forum is one
giant safety meeting. I do have to say that I have not observed one
 thing or anyone on here that even remotely done by a chemist. You
basic training in organic chemistry is lacking. I see very basic
chemistry training at best. You forgot some stuff in the acetate ester
synth. Just saying. You also lack all the proper equipment to perform
what you are doing. Suck it up and get a chemistry degree. Save your
pennies, find someone with chemistry experience and then tackle your
dream. Also get a really good fucking lawyer. Your going to need it.
Even then you will still need the DEA permits. Prohibition ends soon,
but it is not over. Do not push it. It might wreck all the hard work
everyone has put into subverting the system. Regards The King of
Hemp

Reply

24.

Mr. Honest
February 06, 2015 at 6:59 PM

Does this product cross any legal lines that the conventional extraction techniques do
not? Is the federal monitoring of the chemicals a big concern?

Reply

1.

skunkpharmresearch
February 10, 2015 at 8:38 AM

I would encourage you to run it by your own attorney for the answer to
that. We've used less than a liter of acetic anhydride in the last four
years, and have only made it for medical purposes, so not sure what
would happen under adult recreational, or if someone started using
large quantities. It is also used to synthesize Heroin, so is indeed on
the watch list.

Reply
 1.

Name AmeN
May 09, 2015 at 3:43 PM

Depends have you ever had over, 5 agencies raid before?

Reply

25.

Steve-O-Acetate
February 01, 2015 at 1:34 AM

Have you tried using butane as your alkane solvent instead? My organic chemistry
classes would suggest that it would work, however butane is generally more volatile....I
guess my concern is safety...Any thoughts?

Reply

26.

tehcook
October 03, 2014 at 7:03 AM

Hello, You are speaking of converting THC to THC-acetate by using the side-product
acidic acid. Could the THC-acetate also be made without using the acidic anhydride, by
reacting THC and acidic acid with sulphuric acid as catalyst? I'm very curious if it could
be done without the need of acidic anhydride, because it is hard to get and most people
don't like being closely monitored ;) Kind regards from the Netherlands

Reply
 1.

Steve-O-Acetate
February 01, 2015 at 2:04 AM

Theoretically Acetic Acid can cause the esterification of THCA but this
esterification reaction is far less favorable as it is reversible - you now
have H2O in the equation and the presence of water will lead to
hydrolysis of the THC-O-Acetate back to THCA. ....your yield is going
to be low and probably not worth the trouble!....but if you are that
curious give her the old college try!

Reply

2.

Steve-O-Acetate
February 01, 2015 at 2:10 AM

Oh and if you do decide to try it expect the reaction to be much much

slower than with acetic anhydride....just a tip!

Reply

27.

Kevin Roelofs
July 23, 2014 at 7:06 AM

Just curious, but isnt CBD its own analog? You mention CBD converting to THC, but as
I understand CBD is created from its own synthase, using Cannabigerlolic Acid as its
catalyst.

Reply
1.

sharplystupid
August 21, 2014 at 9:45 PM

Without looking into my notes, I believe CBD losses a propyl at some

point and isomerizes to delta-9 ("Potency", 1970s? see 'isomerization')

Reply

1.

Joe
September 11, 2014 at 12:25 PM

I tgought it was CBN that isomerizes into THC

Reply

1.

tehcook
October 03, 2014 at 6:42 AM

If a strong acid gets added to CBD it is converted to THC,

which is what happens when the acidic anhydride gets
added to the oil mixture. THC can turn to CBN under
different conditions, but that usually only happens by
extensive heating, UV-light or being in contact with oxygen
for a long time.

Reply
28.

Jorge_C
July 03, 2014 at 4:12 AM

How is the water solubility of THC-O-Acetate, any better than THC(A)? Comparable to
THC-O-Phosphate?

Reply

29.

BhoDoc
July 01, 2014 at 8:40 AM

Is acetic acid able to be used instead of the acetic anhydrous?

Reply

30.

adelina
January 08, 2014 at 9:50 PM

I have breast tumor and lump and I heard that THC is a cancer cure.I want to buy some
if you have.Thanks!

Reply

1.

mylkingtreez
 May 24, 2015 at 11:13 AM

Officer, there is nothing for sale here! This is a non profit information
based community. Ask your government for help. Good luck with your
lump!

Reply

31.

Anthony Jonovich
December 21, 2013 at 3:49 PM

What is the dry ice in the cooler used for? Is it for the ice water bath or the condenser?

Reply

32.

The BEST Hash Ever - Page 12

October 20, 2013 at 5:16 PM

[…] Anyone can Google isomerizing weed Or better yet

http://skunkpharmresearch.com/thc-acetate/ This has pics!! And I think the person may
have done it before Reply With […]

Reply

1.

skunkpharmresearch
October 24, 2013 at 8:25 AM

Yup, we have.
 Reply

33.

tom
October 19, 2013 at 12:14 PM

I don't know if its the more i read the more stupid i feel? or the smarter i am getting? this
is a lot of info to absorb. I think i need a safety brake.

Reply

1.

skunkpharmresearch
October 20, 2013 at 8:57 AM

Stay safe out there!

Reply

34.

Hexane and soxhlet and other questions - Page 2

October 16, 2013 at 5:28 AM

[…] It is worthwhile to acetalize using sulfuric and acetic anhydride, which you might
check out athttp://skunkpharmresearch.com/thc-acetate/ Originally Posted by
nocturnalreef Thank you first of for actually understanding and […]

Reply

35.
Mary Salas
August 05, 2013 at 3:09 AM

I don’t even know the way I ended up here, but I assumed this post used to be great. I
don't recognize who you are but definitely you're going to a famous blogger when you
are not already ;) Cheers!

Reply

1.

DEA IMNOTA WORRY

September 29, 2013 at 6:30 AM

where the hell do i buy a pump for this i got everything but a pump to
keep the condenser cool

Reply

1.

skunkpharmresearch
September 29, 2013 at 9:36 AM

Aquarium pump with high flow rate.

Reply

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