Scribd Logo
Upload

Welcome to Scribd!

  • Molbio Reporting 1

    Uploaded by

    monzon.mika1801
    2 views2 pages
    Class II biosafety cabinets are the most commonly used type and are divided into four groups - A1, A2, B1, and B2. They provide protection for the personnel, environment, and sample. Class II Type A2 cabinets exhaust 30% of air and recirculate 70% through HEPA filters, while Type B1 cabinets exhaust 70% of air. Class II Type B2 cabinets exhaust 100% of air through HEPA filters before release. Class III cabinets provide maximum containment and protection for working with lethal agents, exhausting 100% of inward and outward air through HEPA filters.

    Original Description:

    Original Title

    MOLBIO REPORTING 1

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    Class II biosafety cabinets are the most commonly used type and are divided into four groups - A1, A2, B1, and B2. They provide protection for the personnel, environment, and sample. Class II Type A2 cabinets exhaust 30% of air and recirculate 70% through HEPA filters, while Type B1 cabinets exhaust 70% of air. Class II Type B2 cabinets exhaust 100% of air through HEPA filters before release. Class III cabinets provide maximum containment and protection for working with lethal agents, exhausting 100% of inward and outward air through HEPA filters.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    2 views2 pages

    Molbio Reporting 1

    Uploaded by

    monzon.mika1801
    Class II biosafety cabinets are the most commonly used type and are divided into four groups - A1, A2, B1, and B2. They provide protection for the personnel, environment, and sample. Class II Type A2 cabinets exhaust 30% of air and recirculate 70% through HEPA filters, while Type B1 cabinets exhaust 70% of air. Class II Type B2 cabinets exhaust 100% of air through HEPA filters before release. Class III cabinets provide maximum containment and protection for working with lethal agents, exhausting 100% of inward and outward air through HEPA filters.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 2

    BSC CLASS II - These are the MOST Class II Type B2 Biosafety Cabinet: All

    COMMONLY USED BIOSAFETY CABINETS; exhaust air is filtered before release,


    This class of cabinet is divided into four preventing contamination of the laboratory
    types: A1, A2, B1, and B2 environment.

    • Is divided into four groups • 100% of the air is exhausted to the


    differentiating based on their airflow environment after passing through
    patterns and exhaust configurations. HEPA filters.
    • Unlike class I, this can provide • low to high-risk biological agents,
    protection towards the personnel, including those requiring Biosafety
    environment and the sample itself. Level 3 containment. Also used for
    toxic chemicals.
    Class II Type A2 Biosafety Cabinet:
    • used for work with high-risk
    Provides personnel protection through a
    biological agents and toxic
    constant inflow of air across the work
    chemicals, as they provide maximum
    surface, creating a barrier between the user
    protection by exhausting 100% of
    and the contaminated materials.
    the air to the environment.
    • 70% of the air is recirculated through
    Class III Biosafety Cabinet:
    a HEPA filter, and 30% is exhausted.
    • are commonly used for general • is the highest level of containment
    laboratory applications and are among biosafety cabinets. WORKING
    suitable for work with biological WITH LETHAL BIOLOGICAL AGENTS
    agents that require Biosafety Level 1, AND TOXINS (like bacteria, viruses
    2, or 3 containments. that can cause potential deadly
    • low to moderate-risk biological disease) typically those requiring
    agents. Not recommended for work Biosafety Level 4 (BSL-4)
    with volatile or toxic chemicals. containment.
    • provide maximum protection to
    Class II Type B1 Biosafety Cabinet:
    personnel, the environment, and the
    Provides personnel protection through
    product being manipulated inside
    inflow and downflow air, ensuring a barrier
    the cabinet.
    between the user and the work surface.
    • The entire cabinet is sealed to
    • 30% of the air is recirculated through prevent any leaks or releases of
    the HEPA filter, and 70% is exhausted hazardous materials.
    to the environment. • The airflow within a Class III BSC is
    • low to moderate-risk biological maintained through a supply and
    agents and some volatile chemicals. exhaust system that ensures a
    (Some volatile chemicals are non- unidirectional flow. All exhaust air is
    toxic and may be compatible with a HEPA-filtered before being released
    Type B1 BSC.) into the environment.
    "Donning" and "Doffing" – are
    terms used in the context of wearing and
    removing personal protective equipment
    (PPE)
    • Donning refers to the process of putting
    on PPE.
    GOWN
    MASK
    GOGGLES OF FACE SHIELD
    GLOVES
    • Doffing refers to the process of taking off
    PPE.
    GLOVES
    GOGGLES OF FACE SHIELD
    GOWN
    MASK
    PROPER HANDWASH

    You might also like