ANGELES UNIVERSITY FOUNDATION

College of Allied Medical Professions

Department of Medical Technology

PERFORMANCE RUBRICS

SUBJECT: MTIS (Immunology and Serology) GROUP NUMBER:

EXPERIMENT/S: Serial Dilution and Agglutination YEAR & SECTION:

DATE PERFORMED:

Members Signature PPEs Demerit/s ATAQ TOTAL SCORE

Criteria % Excellent Very Good Good Poor No attempt

PRE-ANALYTICAL (20%)

PREPARATION OF MATERIALS & WORK AREA

● Assembly of materials is organized, neat & well-
prepared 5 4.25 3.75 2.5 0
5%
● Cleanliness of working area was maintained
throughout the experiment

SAMPLE COLLECTION
● Collected the test sample with no to minimal
supervision. 5% 5 4.25 3.75 2.5 0
● Collected test sample is not hemolyzed or
lipemic.
PREPARATION OF 3% RED CELL SUSPENSION
• Correct sample was used
5% 5 4.25 3.75 2.5 0
• The computation and preparation were all done
correctly
LABELLING OF SAMPLE and TUBE SET UP
● The collected sample is properly labeled. 5% 5 4.25 3.75 2.5 0
● All tubes were labeled correctly.

ANALYTICAL: PROCEDURES AND TECHNIQUES (40%)

Demonstrated appropriate techniques in performing

SERIAL DILUTION.
• Correct use of micropipette
• Correct volume of solute and diluent were added 20% 20 17 15 10 0
in each test tube
• Correct mixing of solution

Demonstrated appropriate techniques in performing

AGGLUTINATION.
• Correct volume of RCS was added
• Followed the required centrifugation time and
speed
20% 20 17 15 10 0
• The manner of dislodging red cell button is
correct
• Correct technique used in reading agglutination
reactions
 ANGELES UNIVERSITY FOUNDATION
College of Allied Medical Professions
Department of Medical Technology

POST-ANALYTICAL (25%)

RESULTS AND INTERPRETATION

● The computation for dilution is correct
20% 20 17 15 10 0
● Correct grading of agglutination
● The titer was correctly reported and interpreted

POST-EXPERIMENT
● Materials and work areas were cleaned with 5% 5 4.25 3.75 2.5 0
initiative.

FOR INDIVIDUAL ATAQ (15%)

ATAQ
● Answers to the questions were complete, 15% 15 12.75 11.25 7.5 0
accurate, and well-explained

LABORATORY WORKSHEET

MTIS (Immunology and Serology)

SUBJECT: GROUP NUMBER:

EXPERIMENT/S: Serial Dilution and Agglutination YEAR & SECTION:

1. Show the complete computation for the dilution of each tube below and
write the final dilution in the table.

a. Computation

b. Final dilution

tube
1 2 3 4 5 6 7 8 9 10 11 12
no.

NSS
0 50 50 50 50 50 50 50 50 50 50 50
(uL)

serum
50 50
(uL)

Final
dilution
 ANGELES UNIVERSITY FOUNDATION
College of Allied Medical Professions
Department of Medical Technology

2. For each test tube, draw the appearance of the final result and write the
agglutination grade.

Tube number Draw the Result Agglutination Grading

10

11

12

3. Identify and report the titer. Interpret the result.

Titer:
Interpretation:

Evaluated by:

__________________________________ _____________
Signature over Printed Name of Instructor Date