` Operation Manual Number : LAC-6205SPE(4)

Edited : March 2010

November 2011
January, 2013
November, 2015

Front Loading Steam Sterilizer

Model LAC-6205SP

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Thank you very much for purchasing DAIHAN LABTECH. Co., LTD products
In order to use the product correctly and most appropriately, Please carefully read and fully understand
instructions in this manual and keep it in a convenient location for further reference.
In order to protect the human life and property, please pay attention to the following sings.

Warning
WARNING
This mark indicates precautions that must be taken if there is danger of electric shock, fire, etc.

which could result in loss of life or injury

Caution
CAUTION
This mark indicates that if these precautions and operation procedure are not taken, damage to
the equipment may result.

Note
This mark indicates that all precautions should be taken for safe usage,

Hot Surface
This mark indicates that burning injury by hot surface may result.
HOT

Explosive

This mark indicates that explosion high pressure may result.

WARNING

‐ This equipment must be used in accordance with the specifications to prevent fire or damage to equipment.

‐ This equipment is not intended for use in locations subject to flammable or explosive gases.

‐ Do not touch high-voltage connections such as power supply terminals, etc. To avoid electric shock.

‐ DAIHAN LABTECH is not responsible if this equipment is repaired, modified of disassembled by other than
factory-approval personnel.

‐ Clogging lines, Equipment malfunction of Failure in the steam supply may cause the autoclave chamber to fill
with scalding water. If the water leaks from any part of the autoclave, do not open the chamber door burns
from scalding water may otherwise result.

‐ Potential exposure to infectious agents through either airborne, droplet or contact transmission.

‐ Do not open the chamber door at any time until the pressure gauge indicating ‘0’

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 CAUTION

● All precaution described in this manual should be taken to avoid damage to the equipment.
● All wiring must be in accordance with local codes and regulations.
● Prevent metal fragments or lead wire scraps from falling inside equipment case to avoid electric shock, fire of
malfunction.
● For proper operation of this equipment, provide adequate ventilation for heat dispensation.
● Turn off the power supply before cleaning the equipment.
● Do not use a volatile solvent such as paint thinner to clean the equipment. Deformation of discoloration will
occur. Use a soft, dry cloth to remove stains from the equipment.
● To avoid damage to equipment display, do not rub with an abrasive material or Press front with a hard object.
● Do not refill the water in the reservoir in operation.
● When processing the Liquids, use only flasks and self-venting automatic sealing stoppers recommended by
the manufacturer. This will help to prevent liquid in the glass containers from rupturing.
● Waster that contains bleach may harm an autoclave.
● Carefully prepare items for autoclaving loosely cover or cap containers to avoid over-pressurization.
● Keep the loads appropriately- overloading hinder the steam penetration.
● Bags should be opened, contained within the stainless steel tray or basket.
● Please an autoclave on the plat and leveled surface.
● Be sure that connect the drain and steam ventilation valve to the drain sink.
● Use distilled water only to protect the heater and sensor.
● Change the water in the chamber frequently to prevent contamination and odor

HOT

● Do not touch the part of top the autoclave or the cover.

‐ Cause by contact with the outside of autoclave.

‐ Caused by contact when removing autoclaved items

‐ Cause by contact with pressurized steam

● Keep away from the autoclave at least 50cm during operation to prevent burning caused by leaking of the
steam.
● After ventilated completely, open the chamber door about one centimeter and allow ten minutes to let the load
cool before removing tray.
● All steel parts of an autoclave are hot. Do not touch during operation or sterilization process.

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● The figures, diagrams and numeric values used in this manual are only of the purpose of illustration.
● DAIHAN LABTECH is not responsible for any damage or injury that is caused as a result of using this
equipment, equipment failure of indirect damage.
● Not portion of this document may be reprinted, copied, stored, and retrieved through any electronic, optical or
other means with prior written approval from DAIHAN LABTECH.
● Do not remove the ground prong(third pin)from the power cord, or use an ungrounded adapter. The autoclave
requires a 3-wire receptacle. If one is not available, consult with an electrician for installation.
● To reduce the risk of electric shock, do not open the body, no user serviceable parts inside.

● Never sterilize the nitrocellulose tubes- they can explode!

● Do not put flammable, volatile or explosive materials inside of the chamber.

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1. Table of contents

1. Table of contents ………………………………………………………………………………5

2. Beginning…………………………………………….………………………………………5
3. Characteristic…………………………………………………………….……………………6
4. Specification and diagram of product……………………………………………………8
5. preparation …………………………………………………………………………..………10
6. use……………………………………………………………………………………………15
7. Operation ……………………………………………………………………………………19
8. Trouble shooting…………………………………………………….…………………..……21
9. Maintenance …………………………………………………………………………………23
10. Quality warranty………………………………………………………………………………24
APPENDIX Ⅰ(P&I schematic) ………………………………………………………25
APPENDIX Ⅱ(Electric circuit)………………………………………………………………26
APPENDIX Ⅲ(Inspection Log)………………………………………………..…………27
APPENDIX Ⅳ(Use List)…………………………………………………………..…………28

2. Beginning

Dear First Time User,

Your Autoclave/Sterilizer using high temperature and pressure steam.
If you are not trained for Autoclave usage or not familiar with Autoclave, please do not operate before you are
trained or completely read this manual and understand about your Autoclave for your health and safety and to
prevent personal injury.
If you do not understand, please contact your laboratory supervisor or technician for further training cycle or
advise.

Autoclaves are such a familiar feature in many laboratories that it is easy to forget what hazards they can pose. The
autoclave’s job is to render its contents sterile, or free of any living organisms. If it fails to do so, serious health hazards
can result. The hot, pressurized steam (135℃, 2.65 kg/㎠ in gauge) that autoclaves generate to do this job makes them
serious burn hazards as well. And, because conditions created inside steam autoclaves are so extreme, autoclaves can
easily malfunction if they are not carefully.
Before using DAIHAN LabTech LAC-Series autoclave at first, read and understand the operating manual thoroughly.
If you have not kept the manual, contact a local distributor or us directly to have a copy.

DAIHAN LABTECH CO., LTD. IS NOT RESPONSIBLE FOR ANY DAMAGE OF PROPERTY OR
PERSONAL INJURY FOR ABUSE OR USAGE FOR WRONG PURPOSE OF AUTOCLAVE.

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3. Characteristic

¾ Convenient use

For your convenience, automatic irrigation system and drainage system is installed and after sterilisation, in a row
without waiting time can be used

¾ Elevator automatic door

As the UP & DOWN sliding door automatically opens and closes with the touch of button.

Method to gasket levitation- sealed door, there are less concerns about burn, because users do not have to control
it by themselves. You can check the condition of door at the LCD display.

¾ Automatic water supply equipment.

As the automatic water supply system is built in, an amount of water provides into chamber to supply constant
water. It is economic, because of water saving system inside.

¾ 5 types of safety equipments.

- (Pressure safety valve)

- (Thermostat)

- (Electronic Limiter)

- (LCD display & Warning buzzer)

- (Door Lock system)

¾ All automatic CONTROL

All automatic control method by Dual 8Bit Processor, there is no mechanical set point, as there are self-check and
self-control, we increased the durability.

If you press start button after closing the door, Preheating > pre- vacuum > heating > sterilization > ventilation >
dry > completion , full automatic process.

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¾ Self-check
There is self-check and self-control function, if equipments damage or parts defects is happened, with alarm sound,
the machine indicate the error no.(refer to 21, 22 Page)

¾ Safety equipment
If pressure insider the chamber is found, then door is not opened.
- when the pressure over 0.03Mpa (0.3Bar)
If the pressure inside the chamber increases then, safety valve will be opened automatically
- When the steam pressure over 2.5bar
Because of maintaining a constant pressure sterilisation, sterilization system is of excellent quality.
- While sterilizing cycle is in progress, steam pressure deviation over ±0.3BAR, then error will be shown.
Prevent from over current by ELCB(Earth leakage circuit breaker)
Self-diagnostic feature tells users the status of a sterilizer.
- Sterilization of the power-on self-diagnosis system has been working from the moment a problem occurs with
the LCD screen displays an error alarm will sound.
During mechanical error or power failure, automatically, pressure will be exhausted from the product.
Heater safety mode
- After finishing cycle or error, unless you press the STOP button, heater is not working.

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4. Specification and diagram of product

1. power of spending : SP Type-12kw

2. Chamber pressure of use : 1.1~2.1bar
3. Chamber temperature of use: 121℃, 134℃
4. Sterilizer course : Unwrapped 1 & 2, Wrapped 1 & 2, Bowied & Dick Test, Liquid
5. diagram per modes

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 z SPECIFICATIONS
MODEL LAC-6205SP/ LAC-6206ES

Overall Size W730 x D1330 x H1870

Size Ø500 x D950

Square Door
Chamber Type Round Chamber
Stainless Steel, W/Jacket

Capacity 186.00 Liter

Heater Power Built in, 20Liter 12kw

Door Type Auto / Manual Vertical Sliding Door

Water Source Service Pipe

Air Removal Vacuum / Gravity

W400 x L1000
Size
Tray W270 x L1000
Material STS 304
Temperature
121℃ ~ 135℃ (1.1 ~ 2.3 bar)
Sterilization Range
Course Unwrapped 1&2, Wrapped 1&2, B/D Test, Liquid

Power Consumption AC380/220V 3Ph 4Line 50/60Hz

Safety Device Safety Valve & Thermostat

Control 8 Bit Microprocessor

Vacuum Type Water Ejector

Drain Source Steel Pipe 15A or more

Printer Standard

Weight 750kg
* This specification could be changed to improve the product without any notice to customer

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5. preparation

5.1. Notice for safety

※ Please read them carefully for your safety and proper use.
z Wear the protection glove z Door handle
The inside of the equipment and sterilizing water Do not attempt to open the door while
is hot after sterilization. There is a possibility of working. If you open the door forcefully, as
burning, so wear protection gloves. pressure comes out, you could get burn

z Be cautious of flammable material and impurities z When sterilizing liquid.

Volatile, flammable, explosive, corrosive and toxic Use only in a liquid‐only cycle.
materials must not be sterilized. Do not use liquid such as saline solution
Impurities such as tape and metals must no enter that
the chamber or water supply entrance. Corrodes metals.
Use a lid that is vented and use PYREX glass
bottle.

z When an ERROR occurs z Impact or movement prohibition

Check the ERROR NUMBER when an ERROR occurs while working, you must not give any
and turn the equipment OFF and check the impact on product or move them
required management procedures immensely
If the ERROR can’t be fixed, contact a nearby
company agent or our company and we will repair
it immediately.

z Press power button while working z Prohibition of overlapping wiring.

Do not use the power button while working, it Certainly, Independent wire should be used.
could be the cause of error or problem Use over rapping wiring may present a fire
risk.

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5.2. Installation and other cautions
※ Please read the instruction before use for user safety and correct use.
z Caution during installation
1. Do not install in an inclined or bumpy area.
2. Install the sterilizer horizontally and approximately 30cm away from the wall.
3. Establish minimum spacing required for inspection and repairs.
4. Do not leave flammable or volatile materials near the sterilizer such as gasoline.
5. Do not put anything on top of the sterilizer.
6. Power supply should be wired with 3Phase 4wiring and above50A circuit breaker.
z Use proper power voltage z Do not dismantle / remodel
Check for a power line that is at least 220V/2.0KW Do not dismantle, repair. Or remodel unless you are
(single Phase) and prepare an exclusive line. a repair technician.
There is danger of fire, electric shock, and injury
Connect the ground wire for safety. due to abnormal operation.

z When not used for an extended amount of time.
Unplug the power cord from the outlet when not
used for an extended amount of
Time and safe keep it from moisture.
Underground water is can not used.
you used underground water, the underground water is
contains a lot of minerals.
A cause of equipment failure is contains a lot of
minerals.

z Be careful of ruptures z When transporting

Do install in place with possibilities of rupture. Do not lay the sterilizer on its side when moving
When not used for an extensive time The sterilizer a long distance. It is a cause of
Remove the water in the water tank. malfunction.

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 5.3. Name and function of each parts
5.3.1. Front view

LCD display

printer
Chamber
pressure gauge Power switch

Jacket pressure
gauge Chamber

Water pump
pressure gauge

Tray1, 2

door

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5.3.2. Drain view (Rear view)

Supply of
Water Line

Drain Line

Main Power Cable

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5.4. Name and function of indicator

① ⑥

②
⑤

③
④

Number Name Function

1 PRINT button to set the print output (on/off)

2 MODE button to choose sterilization course

3 START start button of sterilization cycle

4 STOP button to end the cycle or to open the automatic door

5 ENTER button of progress and creation in the user mode

6 DOWN button to reduce the time and temperature of sterilization

7 UP button to increase the time and temperature of sterilization

8 LCD monitor indication of situation, temperature, pressure and time of the machine

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6. use
Environment for operation
▪ Transport & Storage condition

- The direction has to be as arrows. Avoid the moisture. Fragile. Do not use hook.
- Temperature of shipping: 0℃ ~ +70℃
- Moisture of shipping : relative humidity 0%~ 95%
- Pressure of atmosphere: 760mmHg

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 z When closing the door

① After input the samples into the

chamber, if you choose the start button of
control panel, then door is closed

② Check whether DR:LOCK is indicated

at the LCD display

z When opening the door

① If you press STOP button of control

panel, then door is opened automatically

② Check whether DR:OPEN is indicated

at the LCD display

* In case of opening the door while cycle is progressing, please press the STOP button.
As the FAIL message is shown at the LCD monitor, door is opened automatically.

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6.1. sterilization and drying time according to the type of sterilizing water and status

Minimal sterilization time

(min)
Type and status of sterilizing water 121℃ 132℃
(250℉) (270℉)
Instrument on the tray with holes without the metal wrapped 15 5

Instrument not wrapped with metals and sutures or other

20 10
porosity material

Instrument slightly covering metals or put on the floor with a

Instruments 20 10
cloth

Instrument slightly covering metals and other porosity material

30 15
or put on the floor with a cloth underneath

Warped with 4 layers of cloth 30 15

Glass
Empty and put upside down 15 5
instruments
Wrapped with paper or cloth 30 15
Dressing
Put in loosely in the sterilizing pipe 30 15
Syringe and
Dissembled 30 15
injector
70ml ~ 250ml 20 -
Liquid
500ml ~ 1000ml 30 -

Rubber glove Wrapped in cloth or paper 20 -

Not wrapped 20 10
MOIST
Individually wrapped with cloth or paper 30 15

Sharp and not wrapped 15 5

Tools
Sharp and wrapped with cloth or paper 20 10

Treatment tray Wrapped with cloth or paper 30 -

※ Times above do not include preheating and preparation times and indicate the sustain time at the temperature.
Reference: Perkins JJ: Principles and methods of sterilization in Health Sciences, 2nded Springfield,IL, Thomas1982P

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6.2. Progress and installation of sterilization

z Installation

Choose the time and mode

1. UNWRAPPED1 [ster:121℃~25min, dry:10min]
2. UNWRAPPED2 [ster:134℃~15min, dry:10min]
3. WRAPPED1 [ster:121℃~20min, dry:15min]
4. WRAPPED2 [ster:134℃~10min, dry:15min]
5. LIQUID [ster:121℃~20min, dry:0min]
6. Bowei-Dick Test [ster:134℃~4min, dry:5min]

Each mode shows the corresponding temperature and set time.

Press the ENTER button to change the set values

①Use the Enter button to position the CURSOR to the corresponding part and change the values by pressing the
Down/Up KEY.

* Changed setting is saved, please check it at the next sterilization!

② Limited temperature
temperature Time of sterilization Drying time
MAX 135℃ 30min 60min
MIN 121℃ 0min 0min

z Progress and status display

- When it has been progressed, WAIT is displayed on the top of the LCD monitor and the set CYCLE is progress
automatically.
- The solenoid valve is ON/OFF By setting pressure ( ±0.1BAR), then maintain constant temperature and
progress the sterilization.
-When the CYCLE is complete, END is displayed on the LCD and the end bell rings

*If you want to record the sterilization process, activate the PRINT button before starting to print the full process of

sterilization.

- If the STOP button was pressed or the equipment is re-operated after power failure, FAIL is displayed.
(Remove remaining pressure)
z Re-operation
- After sterilization, push the STOP button.
And then, heater can be re-operation (prevention of overheating)

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7. Operation
■ LAC-6205SP / LAC-6206ES STEAM STERILIZER

1. Turn the main switch on

¾ When the power supply does not turn on, check the circuit breaker which located on the back panel.( Turn
the circuit breaker ON)

2. Water is automatically supplied.

¾ When water level indicator on the LCD is showing over 2 scales, sterilization can be started.
¾ When water level indicator is showing less than 1 scale, don’t push the start button. It will cause errors
3. Put the materials to be sterilized into the chamber depending on the required temperature.

IF the sterilizing water comes into contact with the left/right side, there is a possibility of the material becoming
wet, so be careful for the water not to come into contact.

4. Push the MODE key and choose sterilization procedures.

① User MODE operation method.
- Choose MODE what you want to change.
- Push the ENTER button (First)
- Cursor is blinking on the Sterilization temperature. Set the temperature with UP/DOWN button. (121~135℃)
- Again, push the ENTER button.(Second)
- Cursor is blinking on the sterilization time. Set the sterilization time with UP/DOWN button. (0~30min.)
- Again, push the ENTER button.(Third)
- Cursor is blinking on the dry time. Set the dry time with UP/DOWN button. (0~60min.)
- For the last step, push the ENTER button and setting is completed
9 Randomly user selecting Sterilization temperature and sterilization time, dry time was saved the memory.
9 Please check the setting values before using the sterilization.

5. Operation begins closing the door when pressing the START button.
When sterilization, as follow process by built in Program

① WAIT ⇒PRE-VACUUM ⇒ HEAT ⇒ STERILIZE ⇒ EXHAUST ⇒ DRY ⇒ END

(If there are problems during the procedures, cycles will be stopped and ERROR NO. will be showed in LCD
with buzzer sound.)
② Stop the Sterilization cycle

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 If you want to stop the sterilization, Press the STOP button.

When sterilization is stopped by ERROR or STOP button, please wait until the door open after exhausting
pressure.

6. When melody rings after cycle is finished, push the STOP KEY button.
9 The melody will be stopped after 1 minute.
9 When the cycle is ended by the STOP KEY button, it can be used continuously.
9 When the cycle is ended without the STOP KEY button,
9 In case of end after do not push STOP KEY,
- Æ It needs some times for preheating and STOP KEY should be pushed to set the equipment.
7. When door is opened automatically, please take out the samples.

WARNING

The inside of the equipment and sterilizing water is hot after sterilization.
There is a possibility of burning, so wear protection gloves.

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8. Trouble shooting

z Please check the below

Situation What to check for What to do

▶ Does the lock wheel system turn when

the sliding handle is inserted into the
When the door doesn’t ● Contact the A/S center if the lock wheel
locking system?
close system doesn’t turn.
▶ Is the lock wheel system displayed as
CLOSE?(White, round point)

▶ Is the door open?
If the equipment stops
▶ Is the power turned off?
during operation
▶ Is an ERROR displayed?
● Turn the power OFF and then turn it ON
again. Contact the A/S center if it
doesn’t return to normal.

● This is due to the pressure.

▶ Does the power switch off during
If the door doesn’t open ● Push the STOP button when the
operation? power is on and wait.

● Please supply water. If the situation

What if ERROR NO : ▶ Is there a sufficient amount of
continues after supplying the water,
C1 is displayed? water? contact the A/S center.

▶ Are there any impurities on the gasket?
If vapor leaks through
▶ Is the door completely closed and
the door
locked?
● Check if the lock wheel system is
displayed as CLOSE. If the situation
continues, contact the A/S center.

When a knocking sound

● This is the operation sound of the
generates during
solenoid valve. It is not a malfunction.
operation
● Besides the START/STOP button,
When a button can’t be
pressed during operation any other button can’t be pressed during
operation.
If the equipment ● If the inner pressure is high,
ventilates when the
Ventilation is first progressed for safety.
STOP button has been
pressed It is not a malfunction.

If the keys can’t be

pressed after an ERROR ● Press the STOP button once.
occurs

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z TROUBLE SHOOTING

Viewing the ERROR particulars

¾ Please check the following points before calling for repairs
1. Check it the power is connected.
2. If an error occurs, check the error number on the LCD display and contact our company’s engineering
department for repair and management..
※ During apply for a service for inspection of instrument in cable, Please check the Error code.

¾ ERROR NO.

ERR When to CHECK

Contents
NO. Incipient Pre-
Heating sterilization ventilating Drying
stage vacuum

1 Door doesn’t close completely O O O O O

2 Malfunction of temperature sensor1 O O O O O

3 Malfunction of temperature sensor2 O O O O O

4 Malfunction of pressure sensor O O O O O

5 Insufficient in boiler O O O O O O

6 Malfunction of temperature sensor3 O O O O O

7 NONE
Temperature doesn’t rise to its set value within
8 O
60minutes
9 Temperature not retained with the standard temp O

10 Over heating of boiler 160C O O O O O O

When keep out the± 0.5[BAR]
11 O
In the process sterilization.
When vacuum value keep out the
12 O
±10% than pre-vacuum.
When not cycling
13 O
Detect pressure of chamber
14 Insufficient water in reserve tank O O O O O O

¾ If you couldn’t fix the equipment after doing all above mentioned countermeasures, Please apply for a service
to a technical expert.
¾ During apply for a service department, Please let him know of the Error Code & Serial Number.
¾ Above all details will be change for better equipment.

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Overseas 2nd Factory – PT.DAIHAN LABTECH Headquarters – 247-16 Gagok-Ri, Hwado-Eup
JI. Beringin Lot 322, Batamlndo Indusstrial Park, Namyangju-City, Kyonggi-Do, Korea
Mukakuning, Batam 29433, Indonesia TEL : 82-31-593-9561 / FAX : 82-31-593-9563
India Overseas Factory & Incorporative
Tel : +62 770 612277 / Fax : +62 770 612278
DAIHAN LABTECH INDIA PVT.LTD
TEL : +91 11 2811 4662 / FAX : +91 11 2811 4664
E-mail : info@labtech.co.kr Web : www.labtech.co.kr

Quality Warranty
Descriptions Vertical Steam Sterilizer

Model LAC-6205SP

Serial No. 2015122404

Warranty Period 24 Months after purchase

Date of Purchase

Purchase From

WARRANTY COVERAGE

LabTech’s warranty obligations for the products are limited to the terms set forth below:
Daihan LabTech Co., Ltd. ("LabTech") warrants the product against defects in materials and workmanship for a period
of one (1) year from the date of original purchase ("Warranty Period"), providing that the unit is operated according to
the instruction in the operating manual.

The guarantee comprises removal of all damages that arises during the guarantee period and that are proven to be due
to faulty material or poor workmanship.

If a defect arises and a valid claim is received by LabTech within the Warranty Period, at its option, LabTech will (1)
repair the product at no charge, using new or refurbished replacement parts, (2) exchange the product with a product
that is new or which has been manufactured from new or serviceable used parts and is at least functionally equivalent to
the original product.

EXCLUSIONS AND LIMITATIONS

This Limited Warranty applies only to the product manufactured by or for LabTech that can be identified by Name Plate.

LabTech is not liable for any damage to or loss of any products or material stored or tested in the instruments or programs, data, or other information
stored on any media contained within the product, or any non-LabTech product or part not covered by this warranty. Recovery or reinstallation of
programs, data or other information is not covered under this Limited Warranty.

This warranty does not apply: (a) to damage caused by accident, abuse, misuse, misapplication, or non-LabTech products; (b) to damage caused by
service performed by anyone other than LabTech; (c) to a product or a part that has been modified without the written permission of LabTech; or (d)
if any LabTech serial number has been removed or defaced; or (e) if the unit is not used according to its purpose; or (f) no original spare parts are
used.

LabTech warrants replacement products or parts provided under this warranty against defects in materials and
workmanship from the date of the replacement or repair for ninety (90) days or for the remaining portion of the original
product's warranty, whichever provides longer coverage for you. When a product part is exchanged, any replacement
item becomes your property and the replaced item becomes LabTech’s property. When a refund is given, your product
becomes LabTech's property.

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APPENDIX Ⅰ
P&I schematic

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APPENDIX Ⅱ
Electric diagram

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 APPENDIX Ⅲ
Environmental Health and Safety Internal
Weekly Inspection Log
Check for the following ;
◈ Chemical indicators supply ◈ Biological indicators supply

◈ Make sure the heater cover at the bottom of the chamber or water in the chamber clean
◈ Leakage of water or steam in pipeline

Date Name and Signature of Inspector Comments

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 APPENDIX Ⅳ
Autoclave Use List
CHEMICAL
NUMBER OF PRINT YOUR
PHONE BIOWASTE INDICATOR
DATE TIME CYCLE BAGS NAME AND
EXT (YES/NO) (If biowaste accept
(If Biowaste) LABORATORY
or rejected)

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