BTY396:BIOSEPARATION ENGINEERING

L:3 T:0 P:0 Credits:3

Course Outcomes: Through this course students should be able to

CO1 :: define the operating downstream process equipments and technologies.

CO2 :: discuss the various separation processes used in biotechnology and allied industries

CO3 :: categorize separation processes for the food and biological process industries

CO4 :: solve the downstream processing problems involving biological and environmental
systems

CO5 :: summarize the components of downstream equipment and explain the purpose of each

CO6 :: express the requirements for successful operations of downstream processing

Unit I
An Overview of Bioseparations : Characteristics of bio product, flocculation and conditioning of
fermented medium
Filtration and centrifugation : Revision of mechanical separation, filtration, Centrifugation

Unit II
Cell Disruption : Cell Membranes, Chemical Methods, Mechanical Disruption

Precipitation : Protein precipitation and its separation, Large Scale Precipitations

Unit III
Extraction : The Chemistry of Extraction, Batch Extraction, Staged Extraction

Unit IV
Adsorption : The Chemistry of Adsorption, Batch Adsorption, Desorption processes

Elution Chromatography : Chromatographic methods based on size,, charge, shape, biological

affinity
Unit V
Ultrafiltration and Electrophoresis : ultrafiltration and electrodialysis, Electrophoresis

Unit VI
Crystallization and Drying : Basic Concepts and Crystal Size Distributions, Batch Crystallization,
Recrystallization, Drying Equipment, Conduction Drying, Adiabatic Drying

Text Books:
1. BIOSEPARATIONS: PRINCIPLES AND TECHNIQUES by B. SIVASANKAR, PRENTICE HALL

References:
1. BIOSEPARATIONS: DOWNSTREAM PROCESSING FOR BIOTECHNOLOGY by PAUL A.
BELTER,E. L. CUSSLER AND WEI-SHOU HU, WILEY

Session 2023-24 Page:1/1