3/28/23, 6:28 PM RESIST-5 O.O.K.N.V.

ST-5 O.O.K.N.V. and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study - PubMed

RESIST-5 O.O.K.N.V. and NG-Test Carba 5

assays for the rapid detection of
carbapenemase-producing Enterobacterales
from positive blood cultures: a comparative
study
G Bianco 1 , M Boattini 2 , S A V van Asten 3 , M Iannaccone 2 , E Zanotto 2 , T Zaccaria 2 , A T Bernards 3 , R Cavallo 2 , C Costa 2

Affiliations
PMID: 32304724 DOI: 10.1016/j.jhin.2020.03.022

Abstract
We prospectively compared the performance of RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays directly from blood cultures spiked with 130
characterized Enterobacterales isolates. Overall, both assays yielded 100% sensitivity to detect KPC-type carbapenemases and OXA-48-like
carbapenemases. Both assays failed to detect KPC-31 and KPC-33, D179Y point mutation variants of KPC-3 and KPC-2, that are deprived of
carbapenemase activity and confer resistance to ceftazidime-avibactam. On blood culture bacterial pellets, NDM- and VIM-type carbapenemases
were detected in 50.0% and 52.2%, respectively, by RESIST-5 O.O.K.N.V. vs 100% by NG-Test Carba 5. The sensitivity of RESIST-5 O.O.K.N.V.
improved to 100% and 95.6%, respectively, by performing the assay on 4-h early subculture.

Keywords: Blood culture; Carbapenemase detection; Ceftazidime-avibactam resistance; D179Y; Enterobacterales; Immunochromatographic assay.
https://pubmed.ncbi.nlm.nih.gov/32304724/ 1/2
3/28/23, 6:28 PM RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study - PubMed

Copyright © 2020. Published by Elsevier Ltd.

https://pubmed.ncbi.nlm.nih.gov/32304724/ 2/2