XN-L E Chap04 Adjustment

Edition: 51
CHAPTER 4 ADJUSTMENT
4.1 Pipette and Piercer Position Adjustment ...................................................................................1

4.1.1 Initial Position ...................................................................................................................2
4.1.2 RBC/HGB Chamber position............................................................................................3
4.1.3 WBC chamber position.....................................................................................................4
4.1.4 Aspiration position (XN-550 and XN-450 only)...............................................................5
4.2 AD Offset Adjustment [AC] .......................................................................................................9
4.3 RBC clog level adjustment ........................................................................................................9
4.4 HGB background level adjustment [BL] ..................................................................................10
4.5 Blood Aspiration Sensor Adjustment ......................................................................................11

4.5.1 Blood aspiration sensor blank level adjustment [BL]......................................................11
4.5.2 Blood Aspiration Sensor Adjustment..............................................................................12
4.6 Sensitivity Adjustment .............................................................................................................13

4.6.1 WDF sensitivity adjustment ............................................................................................13
4.6.2 RET Sensitivity Adjustment ............................................................................................15
4.6.3 RBC/PLT Sensitivity Adjustment ....................................................................................16
4.6.4 HGB Sensitivity Adjustment ...........................................................................................17
4.7 Calibration ...............................................................................................................................18

4.7.1 Whole blood mode (calibrator calibration)......................................................................18
4.7.2 Dilution mode .................................................................................................................20
4.7.3 Body fluid mode..............................................................................................................23
4.7.4 Calibration for RBC-He and RET-He at Customer side .................................................26
4.8 Laser Power Adjustment .........................................................................................................27

4.8.1 Removing the covers......................................................................................................27
4.8.2 Laser power adjustment .................................................................................................28
4.8.3 Replacing the covers......................................................................................................29
4.9 Optical Axis Adjustment ..........................................................................................................30

4.9.1 Removing covers............................................................................................................30
4.9.2 Attachment of Jig _ASSY NO. 407.................................................................................31
4.9.3 Latex preparations..........................................................................................................31
4.9.4 Oscilloscope preparations ..............................................................................................32
4.9.5 Adjustment of front and rear directions of light emitter...................................................33
4.9.6 Adjustment of right and left directions of light emitter.....................................................35
4.9.7 Beam stopper adjustment ..............................................................................................36
4.9.8 Adjustment of front scattering receiver...........................................................................38
4.9.9 Optical axis adjustment: Rough check ...........................................................................39
4.9.10 Optical axis adjustment: Fine check...............................................................................40
4.9.11 Forward scattering DC check .........................................................................................41
4.9.12 Screw lock coating .........................................................................................................42
4.9.13 Attaching the covers.......................................................................................................43
XN-L S/M February 2018

Edition: 51
4.10 Sampler (For XN-550) .............................................................................................................44

4.10.1 Preparation for Adjustment.............................................................................................44
4.10.2 Hand Height Adjustment ) ..............................................................................................46
4.10.3 Hand Position Adjustment (Sample Tube Holder position) ) ..........................................47
4.10.4 Hand Position Adjustment (Left adapter position) ..........................................................47
4.10.5 Hand Position Adjustment (Right adapter position)........................................................48
4.10.6 Hand Sensor Adjustment ...............................................................................................49
4.10.7 Adjusting Sample Tube Holder Position (Barcode reading position)..............................49
4.10.8 Adjusting Sample Tube Holder Position (Micro tube detection sensor position)............51
4.10.9 Adjusting Sample Tube Holder Position (Micro tube aspiration position).......................51
4.10.10 Adjusting Sample Tube Holder Position (Sample tube detection)..................................52
4.11 Adjusting Sample Tube Holder Position (Micro tube detection sensor) (For XN-450) ............53
4.11.1 Adjusting Sample Tube Holder Position (Micro tube aspiration position).......................53
4.11.2 Adjusting Sample Tube Holder Position (Sample tube detection)..................................54
4.12 Replacing the piercer, and Adjustment ..................................................................................56
XN-L S/M February 2018

1CHAPTER 4 ADJUSTMENT
4.1 Pipette and Piercer Position Adjustment

In the menu screen, select "Service
-> "Service/Factory Tools"
-> "Mechanical Adjustment"
-> "Piercer/Pipette Adjustment"
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XN-L S/M 4-1 February 2018

4.1.1 Initial Position
(1) Select "Init. Position".

Press "UP" or "DOWN" until the position of
the piercer (pipette) tip is aligned with the
bottom of the rinse cup.
(2) After completing adjustment, press "Save".

4.1.2 RBC/HGB Chamber position
(1) Remove the cover from Chamber_ASSY NO.149.
(2) Select "RBC/HGB Chamber".
Press "Front" or "Back" to move the position of the piercer tip (needle tip) to the center of the
chamber.
Press "UP" or "DOWN" until the position of the piercer tip (needle tip) is aligned with the top of the
chamber.
(3)) After completing adjustment, press "Save".

4.1.3 WBC chamber position
(1) Select "WBC Chamber".
Press "Front" or "Back" to move the position of the piercer tip (needle tip) to the center of the
chamber.
Press "UP" or "DOWN" until the position of the piercer tip (needle tip) is aligned with the top of the
chamber lid.
(2) When you have completed adjustment, press "Save" and then "Close" to close.

4.1.4 Aspiration position (XN-550 and XN-450 only)
(1) Select "Aspiration Position" (CP type only). Press "UP" or "DOWN" until the position of the piercer
tip is aligned with the top (lower part) of the sample tube holder - (white). (Sample tube holder will
not move automatically. Move the sample tube holder manually as needed.) Record the up/down
pulse. [AJ]
Record the value
（Lower）
2015029 [AJ] 2015056

(2) Adjust the front/backward positions by pressing “Front” or “Back”.
From right view, adjust the position

as shown
（Right side）
(3) When you have completed adjustment, press "Save".
(4) Press “Init. Position”.
Front Back
(5) After checking the piercer operation completes, pull sample set unit.
(6) Adjustment for Sarstedt
1) Select Sarstedt in Container type.
2) Press “Aspiration Position”.
3) Adjust the recorded value in step (1) by pressing “Up” or “Down”.
4) After adjusting the value, press “Save”.

5) Press “Init. Position”.
①
⑤
②
Uｐ
③
Down
Adjust the recorded ④

value
(7) Adjustment for “Raised bottom”
1) Select Raised bottom in Container type.
2) Press “Aspiration Position”.
3) Adjust the recorded value in step (1) by pressing “Up” or “Down”.
4) After adjusting the value, press “Save”.
5) Press “Init. Position”.
①
⑤
②
Uｐ
③
Down
④
Adjust the recorded

value
(8) Adjustment for “Normal”
1) Select normal in Container type.
2) Press Save
3) Press Close close the screen.
② ③

(9) Replace the cover of chamber_ASSY NO.149.

4.2 AD Offset Adjustment [AC]
Perform this sequence after replacing one of the following items.
- PCB No. 30013 (Main PCB)
- FCM Detector
- RBC/PLT Detector
- HDD (Only if mmb backup file cannot be obtained from the former HDD)
"A/D convert Auto" needs to be set as shown below, if the mmb backup file is not available.
mmb backup file A/D convert Auto
Available Unnecessary
Not-available Necessary
(1) Select Service/Factory Tools screen from Service.

- Press Paramater
- A/D converter (Auto)
(2) Press “Execute” and values are updated in a few seconds.

(3) Select Close.
4.3 RBC clog level adjustment

Press "Service"
-> "Sensitivity Adjustment"
-> "RBC Clogs"
in the menu screen.
*When “RBC detector clog” error occurs, ignore it. (Press “Reset Alarm.”)
Enter "100" in "Target", press “Calc”, "Save", and then press "Continue".
Repeat until the RBC Clogs value is within "100±1".
When within the specification, press "Close".
[AC] 2016099

4.4 HGB background level adjustment [BL]
* After turning on the power, wait about 10 minutes to stabilize the LED light intensity, then
perform the following procedures.
(1) In the menu screen, press "Service"

-> "Sensitivity" and select "HGB" with Channel ▼.
(2) On the following screen, confirm whether the Blank value is within the specified range (5000 ±
200). If it is within the specified range, click "Close". If it is out of specified range, adjust the value
according to the following procedure.
5000 ± 200
When Gain value is decreased,
the Blank value increases.
1) Enter a numeric value (0 to 255) in "Blank Gain". Then select magnification x1, x2 or x4 by
pressing button “▼”.
2) Press "Save".
3) Repeat steps 1) and 2) until "Blank" value is within the specified range (5000 ± 200).
4) After adjustment, press "Close".
[BL] 317G078

4.5 Blood Aspiration Sensor Adjustment
4.5.1 Blood aspiration sensor blank level adjustment [BL]

* After turning on the power, wait about 10 minutes to stabilize the LED light intensity, then
perform the following procedures.

-> "Sensitivity" and select "Aspiration Sensor" with Channel ▼.
(2) On the following screen, confirm that the Blank value is within the specified range (5000 ± 200). If
the value is within the specified range, click "Close". If it is out of specified range, adjust the value
according to the following procedure.
5000 ± 200
When Gain
When value
Gain is decreased,
value is
the decreased,
Blank valuethe
increases.
Blank value
1) Enter a numeric value (0 to 255) in "Blank Gain" and select x1 or x2 by pressing ▼ button.
2) Press "Save". ("Blank" value is updated)
3) Repeat steps 1) and 2) until "Blank" value is within the specified range (5000 ± 200).(If Blank
value can not be adjusted within the specified range, please perform step (3) below for further
adjustment.)
(3) If Blank value can not be adjusted within the specified range, when selecting magnification x1 or x2
above, please perform the procedure below for further adjustment.
1) Enter a numeric value (0 to 196) in "Blank Gain" and select x4 by pressing ▼ button.
2) Press "Save". ("Blank" value is updated)
3) Repeat steps 1) and 2) until "Blank" value is within the specified range (5000 ± 200).
[BL] 317G078

4.5.2 Blood Aspiration Sensor Adjustment
(1) Always adjust the blood aspiration sensor blank level before performing this procedure.
-> "Aspiration Sensor".
(3) Analyze sufficiently mixed XN-CHECK Level 2.

(4) Enter "13000" in "Target" and click "Calc".
(5) When the new value appears in "Span", click "Save".
Repeat analysis until the analysis result (Sample-Blank) is "13000 ±500".
(6) After adjusting the value to within the range, click "Close" to close.
Remove the checkmark from "Use aspiration sensor", perform blank analysis (CBC mode), and then
perform blood aspiration sensor blank adjustment again.
*Because there is a possibility that span adjustment caused the previously adjusted blank conversion to
change,

-> "Sensitivity,"
and select "Aspiration Sensor" with Channel ▼.
Repeat until the Blank value is within the specified range (5000±200).
After performing adjustment, restore the check mark to "Use aspiration sensor".

4.6 Sensitivity Adjustment
a. Adjust sensitivity after registering reagent information.

b. Aspiration sensor and HGB blank adjustment must be completed before sensitivity adjustment.

-> "Test"
-> "Mode".
Select the "Analysis Mode : Calibrator" checkbox.
Select the "Continuous : OFF" checkbox.
-> Press "OK".
(2) Press "Back" until you return to the menu screen.
(3) Analyze sufficently mixed XN CAL.
(4) Press “Sensitivity from “Service.”
(5) Enter each assy sheet value into “Target and press “Calc” button.
* For detailed procedure of each parameter, refer to following procedures.
(6) After entering all values, press “Save” button.
(7) Analyze sufficently mixed XN CAL.
(8) After measurement is completed, press “Sensitivity” to confirm that all parameters are within assay
sheet targets. Also confirm that scattergrams are normal.
4.6.1 WDF sensitivity adjustment

(1) In the “Sensitivity”, set "Channel " to "WDF".
(2) Enter the values of XN CAL ASSAY SHEET in"Target" (WDF-X, Y, Z), and press "Calc".
The Gain value changes.
[AU]
[AU] 316F017

[Specifications]
WDF-X : Value of XN CAL ASSAY SHEET (for XN-L series) ±3ch
WDF-Y : Value of XN CAL ASSAY SHEET (for XN-L series) ±3ch
WDF-Z [AU] : Value of XN CAL ASSAY SHEET (for XN-L series) ±2.5ch
2015166 [AU] 316F017

4.6.2 RET Sensitivity Adjustment
(1) In the "Sensitivity" screen, set "Channel " to"RET".
(2) Enter the values of XN CAL ASSAY SHEET in "Target" (RET-RBC-X, Y, Z), and press "Calc".
The Gain value changes. .
[Specifications]
RET-RBC-X : Value of XN CAL ASSAY SHEET (for XN-L series) ±1ch
RET-RBC-Y : Value of XN CAL ASSAY SHEET (for XN-L series) ±3ch
RET-RBC-Z : Value of XN CAL ASSAY SHEET (for XN-L series) ±2ch
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4.6.3 RBC/PLT Sensitivity Adjustment
(1) In the "Sensitivity" screen, set "Channel " to "RBC/PLT".
(2) Enter the values of XN CAL ASSAY SHEET in "Target" (MCV, MPV) , and press "Calc".
The Gain value changes. Press "Save" to save the new value.
(
[Specifications]
MCV : Value of XN CAL ASSAY SHEET (for XN-L series) ±1fL
MPV : Value of XN CAL ASSAY SHEET (for XN-L series) ±0.3fL 2016019

4.6.4 HGB Sensitivity Adjustment
Before performing this step, adjust HGB blank level adjust first.
(1) In the "Sensitivity" screen, set "Channel " to "HGB".
(2) Analyze sufficiently mixed XN CAL.
(3) Enter the value of XN CAL ASSAY SHEET in "Target", and press "Calc".
The Gain value changes. Press "Save" to save the new value.
(4) Repeat analysis. When the values are within the specifications, close the screen.
[Specifications]
HGB : Value of XN CAL ASSAY SHEET (for XN-L series) ±0.2g/dL
(5) After adjusting the value, close with "Close".
(7) Check the HGB blank level is 5000 +/- 200 by refering “4.3 HGB background level adjustment”.

4.7 Calibration
4.7.1 Whole blood mode (calibrator calibration)
Note: [BM]
1) Before performing calibration, confirm that the mode setting is "Normal".
(Procedure: Select "Service" -> "Service / Factory Tools" -> "Test" -> "Mode" in
order) If it is not "Normal", set it to "Normal".
2) In case that XN-550 is in the sampler mode, switch to the manual mode.
(The mode cannot be switched in the sampler mode.)
1. Display the [Calibrator calibration] dialog box.

(1) In the menu screen, press "Calibration" -> "Calibrator Calibration".
[Calibrator Calibration] (analysis) dialog box
(2) Start manual analysis of sufficiently mixed XN CAL.

Calibrator calibration is performed in manual analysis.
• Discretes to be analyzed are set automatically and cannot be changed.
• With RET analysis license: CBC + DIFF + RET
• Without RET analysis license: CBC + DIFF
* If an error occurs during analysis, stop calibrator calibration, clear the error, and repeat the proce-
dure from the beginning.
2. Manual analysis procedure [AJ]

(1) XN-550 and XN-450
• Place a vial in the sample holder.
• Press the manual analysis start switch.
• Repeat analyzing the calibrator 11 times. Mix the calibrator properly while analyzing.
Mix as needed while performing analysis.
[AJ] 2015056 2015134 [BM] 2017117

(2) XN-350
• Insert the pipette until it touches the bottom of the vial, and press the manual analysis start
switch.
• Repeat analysis (total of 11 times)
Mix as needed while performing analysis.
* If the analysis results do not satisfy the criteria below, repeat analysis.
• All analysis results are normal.

• All calibration parameters are the [Limit (%)] value or less.
In the [Calibrator Calibration] (analysis) dialog box, touch the analysis numbers of analysis results
that must be repeated, and with the numbers in the selected state, execute manual analysis again.
(3) When analysis ends, press [OK].

(XN-550/XN-450 only) Remove the vial.
Select the items that you want to calibrate by checking or unchecking the checkboxes. A checkbox
cannot be checked when the criteria below are not met.
1) 80% ≤ New correction factor ≤ 120%
2) New correction factor -Current correction factor ≤ ±5%
3) Distribution range ≤ Maximum range
4) Allowable relative error ≤ Relative error ≤ Maximum allowable relative error
If you want to change an automatically calculated correction value, select the "Modify" checkbox
and manually enter the new correction value.
(4) Check the value in the [Calibrator Calibration] (execution) dialog box, and then execute calibration
with [OK].

(5) Check calibration
After calibration, analyze XN CAL, and make sure the result is within the acceptable limit.
* If any parameters are outside the limit, repeat adjustment.
* "Acceptable limit" is the displayed value multiplied by "Acceptable limit (%)".
* Analysis must be performed in calibrator mode.
The WBC target is WBC-D for the XN-CAL displayed value. 2016018
4.7.2 Dilution mode

1. Instrument settings

(1) Press "Change Mode" at the bottom right of the
menu screen.
The analysis mode selection screen opens.

Select the "Dilution" checkbox, and then close
the screen with "OK".
(2) In the menu screen,

press "Service"
-> "Test"
-> "Mode".
Select the "Analysis Mode
: Calibrator" checkbox.
Select the "Continuous
: OFF"checkbox.
-> Press "OK".
(3) Press "Back" until you return to the menu

screen.
* Make sure that the instrument is in manual mode

(4) Press "Manual" at the bottom right of the menu
screen.
The manual analysis screen opens.

(5) Enter any sample number.
(6) Discreet: CBC + DIFF + RET
(7) and then close the screen with "OK".
2. Analysis
(1) Press "Explorer" at the top left of the menu
screen.

(2) Perform 5 tests using a x7 dilution of XN CAL
(500μl) with CELLPACK (3ml).
(Remove the cap from the sample tube before
analysis.)
* Mix the solution before each test.
3. Checking the correction value

Calculate the correction value and make sure it is within the adjustment range below.
Calculation equation:
"New correction value (%)" ="Display value" ÷ "Average value of 5 tests" × Current correction value (%)
<<Adjustment range : 1000 ± 200>>

Parameters: RBC_PD_CAL, PLT_PD_CAL, HGB_PD_CAL, WDF_PD_CAL, RET_PD_CAL,
RBCO_PD_CAL, PLTO_PD_CAL
Enter the correction values in the parameters of the CAL file.
Entry example : 98.65% ->Round off the 2nd decimal digit and enter 987
-> "Service settings"
-> "Calibration".
<<Parameters>>
RBC_PD_CAL 1000, PLT_PD_CAL1000
WBC_PD_CAL 1000, WDF_PD_CAL1000
RBCO_PD_CAL 1000
RET_PD_CAL 1000, PLTO_PD_CAL1000
1. Select a parameter.
2. Enter the value for the parameter in (Value).
3. Press "Export".
* Be sure to press "Export" each time you enter

a parameter.
4. Close the screen with "OK".

screen.
After calibration, analyze a x7 dilution of XN CAL and make sure the result is within the
acceptable limit.

* Analysis must be performed in calibrator mode and dilution mode.

The WBC target is WBC-D for the XN-CAL displayed value.
2016018
4.7.3 Body fluid mode

1. Instrument settings
(1) Press "Change Mode" at the bottom right of the
menu screen.
The analysis mode selection screen opens.

Select the "Dilution" checkbox, and then close
the screen with "OK".
(2) In the menu screen,

press "Service"
-> "Test"
-> "Mode".
Select the "Analysis Mode

: BF Calibrator" checkbox.
Select the "Continuous

: OFF"checkbox.
-> Press "OK".
* Make sure that the instrument is in manual mode.

(4) Press "Manual" at the bottom right of the menu
screen.
The manual analysis screen opens.
(5) Enter any sample number.
(6) Close the screen with "OK".
2. Perform analysis
(1) Press "Explorer" at the top left of the menu
screen.
(2) Perform 5 tests using sufficiently mixed XN
CAL.
(Remove the XN CAL cap before analysis.)
* Mix the solution before each test.
3. Check the correction value

Calculate the correction value and make sure it is within the adjustment range below.
<<Parameters>>
WBC_BF_CAL 1000
RBC_BF_CAL 1000
Calculation equation:
"New correction value (%)" =
"Display value" ÷ "Average value of 5 tests" × Current correction value (%)
<<Adjustment range :1000 ± 200>>

Parameters : WBC_BF_CAL, RBC_BF_CAL
Enter the correction values in the parameters of the CAL file.
Entry example : 98.65% -> Round off the 2nd decimal digit and enter 987

-> "Service settings"
-> "Calibration".

<<Parameters>>
WBC_BF_CAL 1000
RBC_BF_CAL 1000
1.Select a parameter.
2.Enter the value for the parameter in (Value).
3.Press "Export".
* Be sure to press "Export" each time you enter

a parameter.
4.Close the screen with "OK".

screen.

* Analysis must be performed in calibrator mode and dilution mode.
The WBC target is WBC-D for the XN-CAL displayed value. 2016018
2015057

4.7.4 Calibration for RBC-He and RET-He at Customer side
Note: Before the calibration for RBC-He and RET-He, calibration for RBC-Y, HGB, and RBC has to
have been completed.
1. Run XN CAL five times consecutively, and calculate mean for RBC-He and RET-He.
(for RBC-He)
2. Verify whether the deference between the mean for RBC-He and the target value in the assay
sheet is within ±0.5pg.
3. If the mean for RBC-He exceed the range above, perform the calibration by “Calilbration RBC-
He”.
4. After performing the calibration, run XN CAL again (repeat step 1 - 2).
5. Verify that the data are within the above range. If it fails, repeat the steps until it falls within the
range.
(for RET-He)
6. Verify whether the deference between the Mean for RET-He and the target value on the assay
sheet is within ±1.0pg.
7. If the Mean for RET-He exceeds the range, perform the calibration by “Calibration RET-He”.
8. After performing the calibration, run XN CAL again (repeat step 1, 6).
9. Verify that the data are within the above range. If it fails, repeat the steps until it falls within the
range.

4.8 Laser Power Adjustment
Caution: When adjusting the laser power, always wear laser safety goggles (wavelength: 642 nm).
The adjustment below must always be performed after the laser diode is replaced.
In addition, the optical axis must be adjusted after the laser diode is replaced.
(Refer to 4.9 Optical Axis Adjustment)
<<Required tools>>
▪ Phillips head screwdriver
▪ Laser power meter
▪ Ceramic flathead screwdriver
4.8.1 Removing the covers

(1) Loosen the screws on the board cover and FCM BLOCK_ASSY NO.23 cover, and remove the
covers.
(2) Use tape to hold the cover switch on FCM BLOCK_ASSY NO.23 in the ON position.

4.8.2 Laser power adjustment
(1) Set the laser power meter to 633 nm.
(2) Use the ceramic flathead screwdriver to turn VR1 counterclockwise about 10 times.
(3) Select "Service Menu" -> "Service/Factory Tools" -> "Test"-> "Laser Power", and turn ON the laser.
(4) Place the laser power meter diagonally with respect to the optical axis, and turn VR1 with the
ceramic flathead screwdriver to adjust the laser power output value to the target value.
(5) When you have completed adjustment, press "OK" and turn OFF the laser.

4.8.3 Replacing the covers
(1) Remove the tape from the cover switch, and make sure that an error message appears.
(2) Attach the board cover and FCM BLOCK_ASSY NO. 23 cover.

4.9 Optical Axis Adjustment
Caution:
When adjusting the laser power, always wear laser safety goggles (wavelength: 642 nm).
<<Required tools>>
• Hex wrench (1.5 mm, 2.5 mm)
• Phillips head screwdriver
• Oscilloscope
• Latex for forward scattering 4207A(P/N: CX472678)
• Latex for fluorescent light PS-FLUORED-L2830-4 (P/N: CJ959386) [AA] [AK]
• Jig_ASSY NO. 407 (P/N: AP026516)
4.9.1 Removing covers

(1) Loosen the screws on the board cover and FCM BLOCK_ASSY NO. 23 cover, and remove the
covers.
(2) Use tape to hold the cover switch on FCM BLOCK_ASSY NO.23 in the ON position.
[AA] 316E003 [AK] 316G031

4.9.2 Attachment of Jig _ASSY NO. 407
Follow the steps below to attach Jig_ASSY NO. 407to the main board.
(1) Remove the connector connected to J12.
(2) Connect the connector that you removed from J12 to Jig_ASSY NO. 407.
(3) Connect Jig_ASSY NO. 407 to J12.
4.9.3 Latex preparations

Prepare latex solution.
(The terms "forward scattering latex [1] / [2]", and "fluorescent latex" will be used below.)
<Forward scattering latex [1]>

Dispense about 5 drops of 4207A into 10 mL of CELLPACK DFL.
<Forward scattering latex [2]>

Dispense about 5 drops of 4207A into 10 mL of CELLPACK DCL.
<Fluorescent latex>
Dispense about 2 drops of PS-FLUORED-L2830-4 into 10 mL of CELLPACK DCL. [AA] [AK]
• The undiluted latex solution tends to coagulate. Shake well before dispensing.
• The solvent can only be used on the day it is dispensed.
• Do not add additional undiluted latex solution.
• Since florescence intensity of LATEX PS-FLUORED-L2830-4 will be reduced when exposed to
light, store at 2 - 8 degrees C in a dark place. Do not freeze latex. [AA] [AK]
• After diluting, pour the liquid into a bottle or container that is shielded from light and use that
container.
[AA] 316E003 [AK] 316G031

4.9.4 Oscilloscope preparations
(1) Connect the oscilloscope probe to the test points below on the main board.
<Oscilloscope connections>
TP10 :FSC_L , TP11: A.GND-F -> Oscilloscope CH1
TP3 :SFL , TP2: A.GND-F -> Oscilloscope CH2
(2) Set VOLTS/DIV on the oscilloscope to CH1 FSC: 200 mV/DIV, CH2 SFL:20 mV/DIV.
(3) Set to Time: 1.0 μs/DIV.
(4) Set both CH1 (FSC) and CH2 (SFL) to AC. (Refer to steps [1] to [4] below.)

(5) Set the trigger to CH2 (SFL). (Refer to steps [1] to [5] below.)
4.9.5 Adjustment of front and rear directions of light emitter

(1) Set the oscilloscope to CH2 (SFL).
(2) Select "Service" -> "Service/Factory Tools" -> "Optical Axis Adjustment" -> "Rough".
(3) Check the Gain values of FSC, SFL, and SSC. If a value requires adjustment, enter the number
and click "Send".
FSC Gain : 60 High
SSC Gain : 230 High
SFL Gain : 100 Low
(4) Open the door on the right side.
(5) Dispense 1 mL of fluorescent latex into the reaction unit (center chamber of the 3 chambers) and
press the analysis start button.

(6) Fixing screws: Loosen 2 screws
(7) Adjust the adjustment screw so that the oscilloscope SFL signal (waveform) is at its highest point.
(8) When you have completed adjustment, tighten the two fixing screws while viewing the
waveform.(Torque: 30 cN-m)
(9) Close the "Optical Axis Adjustment (Rough)" screen with "Close".

4.9.6 Adjustment of right and left directions of light emitter
(1) Select "Service" -> "Service/Factory Tools" -> "Optical Axis Adjustment" -> "Fine".
(4) Fixing screws: Loosen 2 screws

(5) Move the adjustment plate left/right so that the oscilloscope SFL signal (waveform) is high and
most well-formed.
(6) When you have completed adjustment, tighten the two fixing screws while viewing the waveform.
(Torque: 10 cN-m)

(7) Close the "Optical Axis Adjustment (Fine)" screen with "Close".
4.9.7 Beam stopper adjustment

(1) Set the oscilloscope to CH1 (FSC).
(2) Set the trigger to CH1 (FSC). (Refer to steps [1] to [5] on the next page.)

(5) Dispense 1 mL of forward scattering latex [1] into the reaction unit (center chamber of the 3
chambers) and press the analysis start button.
(6) Fixing screw: Loosen 1 screw.

(7) Adjust the adjustment screw so that the FSC signal waveform is well-formed and at its lowest point.
(8) After completing adjustment, tighten the 1 fixing screw while viewing the waveform.
(Torque: 10 cN-m)

(9) Close the "Optical Axis Adjustment (Rough)" screen with "Close".
4.9.8 Adjustment of front scattering receiver

(1) Change CH1 (FSC) of the oscilloscope to DC.(Refer to steps [1] and [2] below.)
(2) Select "Service/Factory Tools" -> "Test" -> "Laser Power", and turn ON the laser.
(3) Fixing screws: Loosen 2 screws.
(4) Move the adjustment screw to adjust the CH1 baseline (DC amount) to its lowest point.
* If too far out of position, the DC amount will be reduced.

When moving the adjustment screw, the waveform will change in the order High -> Low -> High.
Move to the position where the center is lowest.

(5) Fixing screws: Tighten 2 screws. (Torque: 10 cN-m)
(6) Press "Close" in the "Laser Power" screen to turn OFF the laser.
4.9.9 Optical axis adjustment: Rough check

(1) Place the cover on FCM BLOCK_ASSY NO.23.

(4) Make sure that the analysis results are within the range below.
(It is acceptable if the FSC distribution does not have a single peak.)
(5) Close the screen with "Close".
4.9.10 Optical axis adjustment: Fine check

(1) Select "Service" -> "Service/Factory Tools" -> "Optical Axis Adjustment" -> "Fine".
(3) Make sure that the analysis results are within the range below.
(It is acceptable if the FSC distribution does not have a single peak.)

(4) Press "SFL/SSC" in the lower left corner of the "Fine" screen to change the screen to "FSC".
(6) Make sure that the analysis results are within the ranges below. In addition, the histogram must be
shown in SSC section at the same time. [BO]
SFL-X SFL-W SFL Gain
N/A 0.20 or less 100 Low
SSC-X SSC-W SSC Gain
70 +/-20 N/A 230 High

[AA] [BO]
(7) Close the screen with "Close".
4.9.11 Forward scattering DC check

(1) Set the tester to "DC" of "V". (V...)
(2) Connect the positive (+) terminal to the FSC test point (TP10).
(3) Connect the negative (-) terminal to the GND test point (TP11).
(4) Turn the laser power ON-OFF, and check that the difference between the two is 1.0 V or less.
[AA] 316E003 [BO] 2018012

4.9.12 Screw lock coating
Coat the screw parts indicated in red with screw lock 1401B.

4.9.13 Attaching the covers
(1) Remove the tape from the cover switch, and make sure that an error message appears.
(2) Attach the board cover and FCM BLOCK_ASSY NO. 23 cover.
(Before attaching the board cover, remove Jig_ASSY NO. 407, and connect the original wiring to J12.)

4.10 Sampler (For XN-550)
4.10.1 Preparation for Adjustment

(1) Remove cover as shown by loosening two fixing screws.
(Only for adjusting barcode reader position.)
(2) Put Sample Adapter onto the sampler adapter holder and close it.
(Only for adjusting Hand height and adapter position.)

(3) Turn ON the main unit power switch.
(4) Start IPU program and logon to show menu screen.
(5) Confirm that the Control Menu in lower right is "Sampler."

* When “Manual” is indicated, switch it to "Sampler" by touching mode switching button.

4.10.2 Hand Height Adjustment )
(1) Select Menu Screen "Service"

-> "Service /Factory Tools"
-> Select "Hand Adjustment"
(2) Touch "Set Position."
(3) Touch "Down" (to descent hand)
(4) Touch "Catch" (to close hand)
(5) Adjust the Rod position so that gap between

the adapter and hand is 1 to 4 mm.
(6) Touch "Left Adapter Position1".
(7) Touch "Down" (to descent hand)
(8) Touch "Catch" (to close hand)
(9) Confirm that the gap between adapter and

hand is 1 to 4 mm.
(10) Touch "Save" and "UP."

Fully tighten nut for rod.
Do not move rod.
(11) Apply lock tight as shown.

4.10.3 Hand Position Adjustment (Sample Tube Holder position) )

(2) Touch "Set Position."
(3) Set regular sample tube onto the Sample

Tube Holder. [BE]
(4) Touch "Down" ->

"Catch" and adjust the position so that
the tube is center of the adapter with
"Back", "Front", "Left" and "Right.“ [BE]
(5) Confirm that the tube is in the center of the adapter by touching "Up" and "Down.“ [BE]
(6) Confirm the tube is smoothly caught and released by touching "Release" and "Touch." [BE]
[BE]
4.10.4 Hand Position Adjustment (Left adapter position)
(1) Put regular sample tube to position 1 of Sampler adpter holder (left).
[BE]
[BE] 2017111

(4) Touch "Down" -> "Catch" and adjust the

position so that the tube is center of the
adapter with "Back", "Front", "Left" and
"Right.“ [BE]
(5) Confirm that the tube is in the center of the
adapter by touching "Up" and "Down.“ [BE]
(6) Touch " Release " and "UP.“
(7) Confirm the tube is smoothly caught

released by touching "Release" and "Touch."
[BE]
4.10.5 Hand Position Adjustment (Right adapter position)
(1) Put regular sample tube to position 1 of Sampler adapter holder (right). [BE]
[BE]
(4) Touch "Down" -> "Catch" and adjust the

position so that the tube is center of the
adapter with "Back", "Front", "Left" and
"Right." [BE]
(5) Confirm the tube is smoothly caught and
released by touching "Release" and "Touch.“
[BE]
(6) Touch "Save", "Release" and "UP."
[BE] 2017111

4.10.6 Hand Sensor Adjustment

->"Service /Factory Tools" Loosen two fixing screws.
->"Mechanical Adjustment" -> Tighten after adjusting.
->Select "Hand Adjustment"
(2) Place 10 mm diameter cylinder by hand and

grab it at narrow part by touching "Catch."
(3) Loosen two fixing screws.

(Light sheild
plate)
(4) Observe the "40" sensor status while moving
light shield plate along the arrow.
<Procedure>
Confirm that the "40" sensor becomes white
while light shield plate moving to left.
Tighten two screws at sensor becomes from
"white" to "Red" while light shield plate
moving to right. [BE]
10 mm diameter cylinder
(5) Touch "Close" to close the screen.
4.10.7 Adjusting Sample Tube Holder Position (Barcode reading position)
(1) Place regular sample tube with barcode label onto Sample tube holder front. In this case, to
face barcode label to the slit of the adapter. [BE]
[BE] 2017111

->"Service /Factory Tools"
->"Mechanical Adjustment"
->Select "Tube Holder Adjustment"[AB]
(3) Touch ID Reader Position.
(4) Adjust the ID reader position so that the

chassis plate and resin surface are flush by
touching "Front" and "Back."
(5) Touch "Save."
(6) Loosen two fixing screws for barcode reader.
(7) Touch "Start" button at "Tube Rotation."
(8) Adjust the barcode reader position for

reading bar code label.Tighten two fixing
screws at Bar-code reading position (LED
illuminates with continuous beep sound.)
(9) Touch "Stop."
(10) Touch "Close" to close the screen and

remove the tube. [BE]
[AB] 2016021 [BE] 2017111

4.10.8 Adjusting Sample Tube Holder Position (Micro tube detection sensor posi-
tion)
-> Select "Tube Holder Adjustment"
(2) Touch "Micro tube detection position."
(3) Adjust tube holder position so that the edge

of the chassis and resin surface are flush by
(4) Touch "Save."
4.10.9 Adjusting Sample Tube Holder Position (Micro tube aspiration position)
(2) Touch "Set Position" and remove the regular

sample tube with barcode label. [BE]
(3) Touch "Micro tube aspiration position."
(4) Adjust sample tube holder position so that the gap between the center of the micro tube holder
and the front surface of the Sample tube clamp is 2 mm by touching “Front” and “Back”.
(5) Touch "Save."

[BE] 2017111

4.10.10 Adjusting Sample Tube Holder Position (Sample tube detection)
(1) Press Manual located in lower right.
(2) Enter Sample number as “b3-1”.

(3) Press OK to close.
(4) Place micro collection tube and press start button. [BE]
Observe from gap between sampler and main unit

Set micro collection
sample tubetube
View point
（Right side）
Start switch [BE]
(5) Check that aspiration is performed at micro collection tube position. [BE]
(6) Remove micro collection tube after check. [BE]
[BE] 2017111

4.11 Adjusting Sample Tube Holder Position (Micro tube detection sen-
sor) (For XN-450)
(2) Touch "Micro tube detection position."
(3) Adjust tube holder position so that the edge

of the chassis and resin surface are flush by
(4) Touch "Save."

4.11.1 Adjusting Sample Tube Holder Position (Micro tube aspiration position)
(2) Touch "Micro tube aspiration position."
(3) Adjust sample tube holder position so that the gap between the center of the micro tube holder
and the front surface of the Sample tube clamp is 2 mm by touching “Front” and “Back”.
(4) Touch "Save."

4.11.2 Adjusting Sample Tube Holder Position (Sample tube detection)
(1) When the sample tube holder is not in front position, sample tube holder open/close switch to opent
the sample tube holder.
Sample tube holder

open/close switch
(2) Press Manual located in lower right.
(3) Enter Sample number as “b3-1”.

(4) Press OK to close.
(5) Place micro sample tube and press start button.

Start switch
Set micro test tube
Observe
(6) Check that aspiration is performed at micro sample tube position.

(7) Remove micro sample tube after check.

4.12 Replacing the piercer, and Adjustment
Piercer that is used
Part number Item name
AN965961 Piercer ASP_ASSY (PM) No. 8
When the piercer operation count exceeds 120,000, [Piercer replacement is required.] appears in the
[Help] dialog box. Continuing to use the old piercer will cause tip wear and may result in breakage or
other problems.
A replacement piercer is not included with the instrument. Contact your local Sysmex representative to
purchase a replacement piercer.
(1) Prepare a metal plate, screws, a new piercer and tie wraps for replacement.
The metal plate, screws and tie wraps are provided with a new piecer.
(2) Touch the [Maintenance] icon in the [Menu] screen.

The [Maintenance] menu screen appears.
(3) Touch the [Exchange] icon.

The [Exchange] dialog box appears.
(4) Touch [Replace Piercer].

The [Replace Piercer] dialog box appears.
[Replace Piercer] dialog box

315L006

(5) Touch [Yes].
The piercer cycle count is reset, the piercer moves to the replacement position, and the instrument
power turns OFF.
Wait until this process finishes.
(6) Turn OFF the main power switch.
(7) Open the right cover.

Release the lock by turning to the left with a flathead screwdriver, and open the right cover.
Example: XN-550

(8) Make sure that the piercer moves.
Make sure that the piercer is in the replacement position shown below.
Example: XN-550
(9) Use nippers to cut the tie wrap holding the tube protruding from the top of the piercer, and remove
the tube.
Caution!
When cutting the tie wrap, take care not to damage the tube. Tube
damage may affect analysis results.
Information
Two layers of tubes are used. Remove both layers at once.

(10) Remove the tubes (x2) from the rinse cup at the bottom of the piercer.
(11) Remove the screw from the right side of the rinse cup (under the piercer) with a Phillips head
screwdriver.
(12) Install the replacement metal plate.

Place so that the slit in the metal plate is aligned with the center hole in the rinse cup and the upper
hole in the piercer.

(13) Tighten the screws (x2) on the metal plate with a Phillips head screwdriver.
Tighten the screw on the lower part first, and then the upper part.
(14) Remove the screws (x2) from the piercer and screw (x1) from the rinse cup with a Phillips head
screwdriver.
(15) Remove the piercer.

Dispose of the piercer that has been removed in accordance with local laws and regulations.

(16) Install the new piercer.
(17) Loosely fasten the rinse cup with the screw (x1), and tighten the screws (x2) on the piercer with a
Phillips head screwdriver.
Keep the screw on the rinse cup loose.
(18) Tighten the screw that was loosely tightened in step 17 with a Phillips head screwdriver.
Tighten the screw while lifting the rinse cup so that there is no gap (A).

(19) Remove the screws (x2) from the metal plate with a Phillips head screwdriver.
(20) Remove the metal plate.
(21) Tighten the screw on the right side of the rinse cup with a Phillips head screwdriver, and attach 2
tubes to the rinse cup.

(22) Attach the tube above the piercer and fasten with a tie wrap.
Be careful not to kink the tube when binding the tie wrap. Cut off the excess tie wrap.
(23) Close the right cover and lock.
(24) Turn ON the main power switch.
(25) Press the power switch.
(26) After the replacement, if any trouble occurs, please refer to [4.1 Pipette and Piercer Position
Adjustment].

XN-L E Chap04 Adjustment

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XN-L E Chap04 Adjustment

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Edition: 51

4.1 Pipette and Piercer Position Adjustment ...................................................................................1

4.2 AD Offset Adjustment [AC] .......................................................................................................9

4.3 RBC clog level adjustment ........................................................................................................9

4.4 HGB background level adjustment [BL] ..................................................................................10

4.5 Blood Aspiration Sensor Adjustment ......................................................................................11

4.6 Sensitivity Adjustment .............................................................................................................13

4.7 Calibration ...............................................................................................................................18

4.8 Laser Power Adjustment .........................................................................................................27

4.9 Optical Axis Adjustment ..........................................................................................................30

XN-L S/M February 2018

4.10 Sampler (For XN-550) .............................................................................................................44

4.12 Replacing the piercer, and Adjustment ..................................................................................56

XN-L S/M February 2018

4.1 Pipette and Piercer Position Adjustment

XN-L S/M 4-1 February 2018

(1) Select "Init. Position".

XN-L S/M 4-2 February 2018

(3)) After completing adjustment, press "Save".

XN-L S/M 4-3 February 2018

XN-L S/M 4-4 February 2018

Record the value

2015029 [AJ] 2015056

XN-L S/M 4-5 February 2018

From right view, adjust the position

(3) When you have completed adjustment, press "Save".

(4) Press “Init. Position”.

XN-L S/M 4-6 February 2018

Adjust the recorded ④

Adjust the recorded

XN-L S/M 4-7 February 2018

XN-L S/M 4-8 February 2018

mmb backup file A/D convert Auto

(1) Select Service/Factory Tools screen from Service.

(2) Press “Execute” and values are updated in a few seconds.

4.3 RBC clog level adjustment

XN-L S/M 4-9 February 2018

(1) In the menu screen, press "Service"

XN-L S/M 4-10 February 2018

4.5.1 Blood aspiration sensor blank level adjustment [BL]

(1) In the menu screen, press "Service"

XN-L S/M 4-11 February 2018

(3) Analyze sufficiently mixed XN-CHECK Level 2.

(7) In the menu screen, press "Service"

XN-L S/M 4-12 February 2018

a. Adjust sensitivity after registering reagent information.

(1) In the menu screen, press "Service"

(2) Press "Back" until you return to the menu screen.

(3) Analyze sufficently mixed XN CAL.

(4) Press “Sensitivity from “Service.”

(7) Analyze sufficently mixed XN CAL.

4.6.1 WDF sensitivity adjustment

XN-L S/M 4-13 February 2018

XN-L S/M 4-14 February 2018

XN-L S/M 4-15 February 2018

XN-L S/M 4-16 February 2018

(1) In the "Sensitivity" screen, set "Channel " to "HGB".

(2) Analyze sufficiently mixed XN CAL.

(5) After adjusting the value, close with "Close".

(6) Press "Back" until you return to the menu screen.

XN-L S/M 4-17 February 2018

4.7.1 Whole blood mode (calibrator calibration)

1. Display the [Calibrator calibration] dialog box.