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  • MMC 1

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    Esdinawan Carakantara Satrija
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    A document describes a genetic engineering experiment involving gene editing of bacteria. It includes the sequence of a targeting vector containing elements like a promoter, repeats, gRNA, and terminator. A graph shows that co-transformation of bacteria with the targeting vector (gRNA) and a homologous DNA template (HDR) resulted in gene knock-ins, while the gRNA alone did not.

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    mmc1

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    A document describes a genetic engineering experiment involving gene editing of bacteria. It includes the sequence of a targeting vector containing elements like a promoter, repeats, gRNA, and terminator. A graph shows that co-transformation of bacteria with the targeting vector (gRNA) and a homologous DNA template (HDR) resulted in gene knock-ins, while the gRNA alone did not.

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    © All Rights Reserved
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    3 views1 page

    MMC 1

    Uploaded by

    Esdinawan Carakantara Satrija
    A document describes a genetic engineering experiment involving gene editing of bacteria. It includes the sequence of a targeting vector containing elements like a promoter, repeats, gRNA, and terminator. A graph shows that co-transformation of bacteria with the targeting vector (gRNA) and a homologous DNA template (HDR) resulted in gene knock-ins, while the gRNA alone did not.

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    © All Rights Reserved
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    of 1

    A B

    cas10

    csm6

    csm3

    GGATCGTCGGCACCGTCACGGCCGTGGGAGGCGGCA
    CGATCCGCGACGTGATGATCGGCCGCATCCCCACGGT
    GCTGCGCAGTGAGCTCTACGCCATCCCGGCGTTGATC
    TGTGCGTTCGCACGCACAGGCCCGGTGTGAGAAGGG D
    TCTGTGACGAGCGGGAGAACCCACCCGGGGTGGGCG
    AGTTTGTCCTGCGTGTGCTCGGTCGAGTAGGCTCTGG
    GAGTACCCGTGTGTACGACCAGCACGGCATACATCAT F R1
    TTCGACGCCGAGAGATTCGCCGCCCGAATCTAGAGTC Left arm eGFP / BFP Right arm
    GTCAGACCCAAAACCCCGAGAGGGGACGGAAACGTC
    TGGTCTGCGCCGTTGGCGTCCACGGCATTATCGTCGC
    GACCCAAAACCCCGAGAGGGGACGGAAACTTCCAGG R
    CATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGG 1.1 kb
    CCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCT
    TTTTT 1.72 kb

    Psmyc promoter Repeats gRNA Terminator

    E F HDR + gRNA

    2000
    Number of CFUs
    (per mL)

    1500

    1000
    gRNA only
    500

    0.0
    gRNA - - + +
    HDR template - + - +

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