Specification

1.03870.0500 Brain Heart Infusion (BHI) agar acc. FDA-BAM GranuCult® prime

Specification

Appearance (clearness) clear to

opalescent
Appearance (color) yellowish-brown
pH-value (25 °C) 7.2 - 7.6

Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Specification

lnoculum on reference medium

(Staphylococcus aureus ATCC 25923
(WDCM 00034))
lnoculum on reference medium (Listeria
monocytogenes ATCC 13932 (WDCM
00021))
lnoculum on reference medium (Enterococcus
faecalis ATCC 19433 (WDCM 00009))
lnoculum on reference medium (Escherichia
coli ATCC 25922 (WDCM 00013))
lnoculum on reference medium (Candida
albicans ATCC 10231 (WDCM 00054))
Colony count (Staphylococcus aureus ATCC
25923 (WDCM 00034))
Colony count (Listeria monocytogenes ATCC
13932 (WDCM 00021))
Colony count (Enterococcus faecalis ATCC
19433 (WDCM 00009))
Colony count (Escherichia coli ATCC 25922
(WDCM 00013))
Colony count (Candida albicans ATCC 10231
(WDCM 00054))
Recovery (Staphylococcus aureus ATCC -:?. 70 %
25923 (WDCM00034))
Recovery on test medium (Listeria -:?. 70 %
monocytogenes ATCC 13932 (WDCM
00021))
Recovery on test medium (Enterococcus -:?. 70 %
faecalis ATCC 19433 (WDCM 00009))
Recovery on test medium (Escherichia coli -:?. 70 %
ATCC 25922 (WDCM 00013))
Recovery on test medium (Candida albicans -:?. 70 %
ATCC 10231 (WDCM 00054))
Growth (Aspergillus brasiliensis (formerly A. weak to good
niger) ATCC 16404 (WDCM 00053))

Merck KGaA The life science business of Merck KGaA, Darmstadt, Page 1 of 2
Corporation with General Partners Germany operates as MilliporeSigma in the U.S. and
Frankfurter Strafse 250 Canada.
64293 Darmstadt, Germany
Specification
1.03870.0500 Brain Heart Infusion (BHI) agar acc. FDA-BAM GranuCult® prime

Incubation:
24 ± 2 hours at 35 ± 1 °C, aerobic
fungi: up to 3 days at 35 ± 1 °C, aerobic

A recovery rate of 70 % is equivalent to a productivity value of 0.7.

The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy agar

Stefanie Fischer
Responsible laboratory manager quality control

This document has been produced electronically and is valid without a signature.

Merck KGaA The life science business of Merck KGaA, Darmstadt, Page 2 of 2
Corporation with General Partners Germany operates as MilliporeSigma in the U.S. and
Frankfurter Strafse 250 Canada.
64293 Darmstadt, Germany

SALSA Version 1155676/000000000000// Date: 14.01.2022