1

System System Manual

Manual version 0.2

version 0.2 February 2007

This section will provide you with a list of the order in which the equip-
ment should be: installed, setup, calibrated etc. After you have com-
pleted a step refer back to this page to see what the next step is.
There will be times when the manuals with need to be used in con-
junction with one another. This list is here to help you use the equip-
ment more efficiently, you should still read all of the manuals thor-
oughly before using this list. By reading all of the manuals and then
referring to this list you will have a better understanding of how the
equipment works.

1. First, adapt the generic chamber. In the “Adapting a Generic Chamber” manual, refer to the:
“Installation Overview” section, for an overview of how to install the generic chamber. Also use
the “Adapting a Chamber for a ProCO2” section for information and directions on how to
adapt the chamber for a ProCO2.
2. Now, installing the ProCO2. In the “ProCO2 Model 120” manual refer to the: “BioSpherix
Supplied Parts” and “User Supplied Parts” sections for a parts list. Also refer to the:
“Setup of Gas Supply”, “ProCO2 Front Panel Installation” and “ProCO2 Back Panel Instal-
lation” for how to make all the connections.
3. Next, familiarize yourself with the push button interface. In the “ProCO2 Model 120”
manual refer to the: “Operation”, “Program Functions Menu” and “Configuration” sections
for information regarding the push button interface.
4. Now, calibration. In the “ProCO2 Model 120” manual refer to the: “Calibration” section
for information on how to calibrate the sensor.
5. Next, tuning. In the “ProCO2 Model 120” manual refer to the: “Tuning” section for spe-
cific information on fine tuning. You shouldn’t have to use this section much, because most
tuning is done at the factory.
6. Now, operating. In the “ProCO2 Model 120” manual refer to the: “Single Setpoint Con-
trol” section for information on how to operate with the ProCO2. Also refer to the: “Setting
the Alarm Setpoint” section for directions for how to set a specific alarm setpoint.
7. Finally, maintenance issues. In all of the manuals refer to; “Maintenance” sections on
how to maintain the respective equipment.

This system consists of many parts. Each

part has it’s own manual. The name of the
each individual manual and it’s version
number are as follows: PO Box 87
19 Demott St.
Lacona, New York 13083
“ProCO2” Manual v1.0 d0207 PHONE 315-387-3414
FAX 315-387-3415
“Adapting a Generic Chamber” Manual TOLL FREE US/CAN 800-441-3414
v0.2 d0506 www.biospherix.com
 1
ProCO2 Model 120 ProCO2 model 120

Manual version 1.0

version 1.0 Feb 2007

This manual is intended to help our customers efficiently

setup and operate the equipment. We encourage not only all
installers, but also all users, to read this manual thoroughly.
Keep it handy and refer to it often. Save it for future
reference. If you have any problems or questions, please do
not hesitate to call. We are here to help.
Introduction
The ProCO2 is a versatile and compact gas carbon dioxide controller for people who do
carbon dioxide sensitive work. Though designed to work with BioSpherix chambers the
ProCO2 works in incubators, glove
boxes, animal cages, refrigerators,
plant growth chambers, and many other
semi-sealable chambers. Practically
any small-medium enclosure can be
fitted. Even cardboard boxes and
plastic bags. The unit works from
outside of the host chamber by re-
motely sensing and displacing the air inside the chamber with CO2.

The nominal range of carbon dioxide concetration is 0.1-20.0%. Advanced feedback algo-
rithms handle practically any carbon dioxide gas dynamic.
Infusion rates are adjustable up to 35 SCFH, sufficient for
most lab chambers. Installation is easy. Operation is simple.
The unit moves easily from one host chamber to another.
Please read and follow the safety and operations instructions
PO Box 87 on the following pages. Be careful. Any pressurized gas can
19 Demott St. be dangerous. Know what you are doing and do it safely.
Lacona, New York 13083
PHONE 315-387-3414
FAX 315-387-3415
TOLL FREE US/CAN 800-441-3414
www.biospherix.com

Anyone who has not thoroughly read and under-

stood this manual, must never attempt to operate
! the equipment. !
 2
Table of Contents ProCO2 model 120
version 1.0

Safety Instructions........................................................................3

BioSpherix Supplied Parts............................................................4

User Supplied Parts.......................................................................5

Setup of Gas Supply......................................................................6

ProCO2Front Panel Installation.....................................................7

ProCO2 Back Panel Installation....................................................8

Operation........................................................................................9

Program Functions Menu............................................................10

Configuration...............................................................................11

Calibration...............................................................................12-13

Tuning......................................................................................14-17

Single Setpoint Control...............................................................18

Setting the Alarm Setpoint..........................................................19

Maintenance.................................................................................20

Warranty........................................................................................21
 3
ProCO2 model 120
version 1.0

SAFETY INSTRUCTIONS

ELECTRICAL POWER SOURCE unit should be connected to a power supply only of the
type described in the operating instructions or as marked on unit.
POWER CORD PROTECTION power supply cords should be routed so that they are not
likely to be walked on or pinched by items placed upon or against them.
ELECTRIC SHOCK do not remove cover of ProCO2 due to presence of uninsulated "dan-
gerous voltage" within product's enclosure.
ELECTRIC SHOCK unit should never be used where it can fall or be pushed into water.
ELECTRIC SHOCK if modifying host chamber, be careful not to drill or cut into electrical
wires hidden behind chamber wall. Never drill or cut blindly.
NONUSE PERIODS power cord of unit should be unplugged from electrical outlet when left
unused for long period of time.
FALLING OBJECTS AND LIQUID SPLASH care should be taken so that objects do not fall
on equipment and liquids do not spill, splash, or drip onto or into unit enclosure or
power cord.
PRESSURIZED GAS secure all connections with hose clamps. Never exceed pressure
limits. Bleed all lines before disconnecting. Wear safety glasses at all times.
LOW OXYGEN ATMOSPHERES never enter a chamber which has a low oxygen atmo-
sphere because of severe danger of suffocation. Host chamber should be in well venti-
lated room. Control gas (nitrogen or other low oxygen gas) continuously leaks out of
chamber and should never be allowed to build up in room outside of chamber.
HIGH OXYGEN ATMOSPHERES never enter a chamber which has a high oxygen atmo-
sphere due to danger of oxygen toxicity. Never smoke or allow any source of fire or
spark in or around a chamber with high oxygen atmosphere. Oxygen radically promotes
combustion and can be explosive. Host chamber should always be in well ventilated
room. Oxygen continuously leaks out of chamber and should never be allowed to build
up in room outside chamber.
VENTILATION unit should be situated so that its' location or position does not interfere
with proper ventilation. Neither ProCO2 nor host chamber should be in poorly ventilated
areas.
HEAT unit should be situated away from heat sources such as radiators, heat registers,
stoves, or other appliances or processes that produce heat.
CLEANING do not immerse unit in water. Do not wipe unit with wet cloth or sponge or
paper. Clean only with a dry cloth.
 4
BioSpherix Supplied Parts ProCO2 model 120
version 1.0

This section will familiarize you with the components that come with
the ProCO2.

ProCO2 unit

12VDC power supply

Calibration chamber
with tubing

Carbon dioxide gas sensor

and sensor cable

Short piece of 1/8 in. ID

tubing
Infusion tubing
Gas Fittings
 5
User Supplied Parts ProCO2 model 120
version 1.0

This section will list the parts that the user will need to supply in order to
operate the equipment.

1. Qty. 2 regulators, either a one stage regulator or a two staged regulator. Either way you
will need two gauges, one for the amount of gas in the tank and one for the gas coming out
of the tank. (2500 PSIG input 0-60 PSIG output, recommended). PSIG stands for Pounds
per Square Inch Gauge as opposed to PSIA which stands for Pounds per Square Inch
Absolute.
2. Qty. 1 compressed gas source (tank) of carbon dioxide.
3. Qty. 1 compressed gas source (tank) of carbon dioxide/oxygen mix (ratio should be 10-
90).
4. Qty. 1 compressed gas source (tank) of nitrogen (optional, see “Calibration” section for
information).
 6
Setup of Gas Supply ProCO2 model 120
version 1.0

This section will describe how to setup the gas supply, for information
on how to connect the gas supply to the machine, see the calibration
sections and the “Single Setpoint Control” section.

Control gas must be supplied through a 1/8 in. ID tube to the back panel of the
ProCO2. If the tubing coming from the compressed gas source (tank) isn’t 1/8 in. then use
one of the provided gas fittings to adapt the tubing to 1/8 in. The pressure must be regu-
lated to 0-25 PSIG. For best results use approximately 2 PSIG in aCulture Chamber and
use 15 PSIG in an Animal Chamber. (refer to “Tuning” section under the “Power” heading
for further information). Do not connect any tubing yet, wait until told to do so in the “Cali-
bration” section and “Single Setpoint Control” section.
The amount of gas used is determined by how the chamber is used, not the ProCO2.
The ProCO2 uses the least amount of gas possible, which is only what the chamber needs.
The amount of gas used is dependent on: (1) The size and leakiness of the chamber, (2)
The amount of times and how long the chamber door(s) are opened, (3) The carbon diox-
ide level being controlled.
Use a one or a two stage, 2500 PSIG input, 0-60 PSIG output regulator at the source
of the compressed gas. It is best to have two gauges so that you can monitor the amount
of gas in the compressed source and also monitor the amount passing through the output.
Never allow the pressure coming out of the compressed source to exceed 25 PSIG or
damage will occur to the ProCO2. If the regulator is not near the ProCO2 then there
should be a shutoff valve placed upstream of the unit. When taking off gas supply tube
always make sure to shut off compressed gas at the source first, then bleed the pressure
out of the line and then take off the tube from back of ProCO2.
Step-by-Step Procedure for Connecting Gas to Machine

1. Before connecting the regulator to the tank, close the regulator and make sure the tank
is also closed off.
2. Connect the regulator to the compressed nitrogen (if applicable), compressed carbon
dioxide and to the compressed carbon dioxide/oxygen mix gas sources.
3. Connect the tubing from each compressed gas source to the ProCO2 when told to do so
in the calibration procedures and the operation procedures.
4. At this point you don’t have to open the regulator or turn on the gas. You will be turning
the gas on at the beginning of the calibration/single setpoint control procedures, for now
just connect the regulator.
 7
ProCO2 Front Panel Installation ProCO2 model 120
version 1.0

The following steps will explain how to set up the ProCO2 front panel
and prepare it for installation to a chamber.
1. Placing the Unit
Place the unit on a level secure surface either on the
chamber or within seven feet of the chamber. Make sure
you can easily access the front panel.

2. Gas Connected Properly

Each time you connect a gas supply, make sure that
the gas is connected properly open the bleed valve by
turning the knob on the front of the ProCO2 counterclock-
wise. If gas is heard expelling out the “BLEED” barb on
the front panel then the gas is connected properly. Once
you have confirmed then close the valve. Do this after 2
4
you have followed the procedure in the “Setup of Gas
Supply” section and are beginning either operation or
calibration.

3. Connecting Calibration Chamber

Attach the calibration chamber by connecting the
1/16 in. ID tubing to the barb labeled “BLEED” on the
front of the ProCO2, when calibrating.

4. Gas and Alarm Switches

Flip the “GAS” switch up to allow the gas to flow to
the chamber. Flip the “ALARM” switch down to enable
the alarm to sound. 3
 8
ProCO2 Back Panel Installation ProCO2 model 120
version 1.0

This section will describe how to setup the back panel of the ProCO2.

1. Power Supply
Plug the jack end into the port labeled “12VDC” on
back of ProCO2. Plug the 12VDC power supply into a
wall outlet. The display on the front of ProCO2 should
turn on.

2. Connecting Gas
Attach 1/8 in. ID tubing to hose barb labeled “IN” on
the back of the ProCO2. Making sure that the gas com-
ing in Never exceeds 25 PSIG or damage will occur to
the ProCO2. Depending on if you are calibrating or
operating will determine what type of gas you will be 1
using. (see “Calibration” and “Single Setpoint Control” 2
sections).
3. Connecting Sensor
Plug the sensor cable into the port labeled
“SENSOR” on back of ProCO2 by twisting the sensor
cable into the port until it pushes in, then push in until
“click is heard. When removing pull the ring back and
pull cable off. Plug the other end of the sensor cable into
the sensor itself by lining up the key on the terminal with
the key on the connector of the sensor cable. Push in
and twist the ring on. The display on the front of the
ProCO2 should respond.

4. Connecting Infusion Tube 3

Attach 1/8 in. infusion tube to “OUT” barb on back of 4
ProCO2.
 9
OPERATION ProCO2 model 120
version 1.0

This section will give an overview of some of the different modes and
functions within the ProCO2

SINGLE LEVEL NAVIGATION

Press up
or down
key once
to move to
the next
function.
Hold up or down keys to automatically
index through the functions. Always
index down to get to level function.

CHANGE LEVELS
Multi-level menu of functions requires
navigating from level to level.
Index down key to
level function.

WORK MODE PROGRAM MODE Release down key

Work mode is the normal day- All configuration, calibration, to display current
to-day operating mode. Whether and tuning operations take place in level.
controlling or not (parked), carbon program mode. You access the
dioxide concentration at the sensor program menu, navigate through it,
is continuously displayed, unless select appropriate options, and exit Press and hold star
pre-empted by other operations. back to work mode. key while using
Operations in work mode are Entry into menu is always at down key or up key
limited to: (1) view setpoint, (2) to select new level.
same point, the "tunE" function on
change setpoint, (3) reset alarm/ level 1. Release star key to
error message, (4) enter program During programming, control display new level.
mode. with existing settings is maintained.
New settings are written to memory
VIEW SETPOINT only upon exiting program mode.
In work mode, Settings are retained in non-volatile VIEW FUNCTION/OPTION
press and hold memory for years even if unit is On release of up or
star key. Setpoint down key at
is shown in
unpowered. function, display
alternating display. Security locks can configure alternates with the
certain options unadjustable. How- option selected.
ever, all functions and locked op-
CHANGE SETPOINT tions may be viewed even when CHANGE OPTION
locked. Index to required function. Press and
Press and hold star hold star key (current option
key. Press up key to Exit program mode from any displayed). Press up and/or down keys
increase setpoint. place in menu. Exiting program to display new setting.
Press downkey to mode returns to work mode with new Release star key.
decrease setpoint. If IMPORTANT: Check
change requires settings.
new selection
control output, SP1 ENTER/EXIT PROGRAM MODE (alternating with
indicator will flash. function) before
Simultaneously hold both up and down
keys for 3 seconds. You enter moving to another
program mode from function or exiting
RESET ALARM/ERROR normal operating program mode.
MESSAGES mode at the tune ENTRY TO HIDDEN LEVEL 4
When the alarm or function on level 1 in At "VEr" function on
error condition has the menu. You exit level 3, press and hold
been cleared, program mode at both upkey and
momentarily press any point in the downkey
both upkey and menu, returning to normal operating simultaneously for 10
downkey at the mode. With exit, any new instructions seconds. To enter level
same time. get entered into memory. 4
 1
4

Ú
Ú
Ø
Read control SP1 Read SP1 control
Select Autotune or Park output device
output power %

×
Proportional Band / Gain SP1 manual power Read SP2 alarm
or Dead band width control % output device

Integral time / Reset

Set SP1 power limit % Burn-out protection
(Automatic offset)

PROGRAM ENTRY POINT

Set SP2 power limit % Select output modes:
Derivative time / Rate
Direct/Reverse
with only a few of the important ones.

Derivative approach Main alarm SP2 SelectSP1/SP2

control mode: strategy. L.E.D. modes
are used only in rare instances. Chances are
similar functions. Some functions are defaults

99.99% you'll have to become intimately familiar

Proportional cycle-time Subsidiary alarm SP2 Sensor span adjust

(hard or soft). Some are read only. Some are only
active depending on other function settings. Some

or ON/OFF mode: latch/sequence

Offset / Manual reset Select display resolution Sensor zero adjust Derivative sensitivity
(decimal point)

Lock main setpoint SP1 Set scale maximum Select control Display sensitivity
accuracy monitor

Adjust alarm SP2 Disable alarm

Set scale minimum Read monitor results
setpoint annunciator
PROGRAM FUNCTIONS MENU

there's one way in. Menu items are loosely grouped together according to
bottom of level 1. Exit menu from any point. Level 4 is hidden for security;
PROGRAM MODE FUNCTIONS MENU is arranged on 4 levels. Entry into menu is

Select SP2 dead Read Autotune Disable program

band width or Select sensor input
tuning data auto-exit
Proportional band

Select SP2 ON/OFF Select units of Software version Security lock

or Cycle-time measurement
×

Functions reset
version 1.0
10
ProCO2 model 120
 CONFIGURATION 11
ProCO2 model 120
version 1.0

Extensive configuration options in alarming, security, and display

functions all can be set later, if necessary. Only one main
configuration setting is required to start carbon dioxide level
SECONDARY
CONFIGURATIONS
Alarm configuration options in-
clude deviation, band, and fullscale.
Output can be reverse or direct act-
ing. Alarm intensity is configurable
by timed pulse proportioning.
Security options against people
errors include set point lock, set
point range limits, and limited menu
access to prevent unauthorized
changes. Burnout contingencies and
output overload limits protect against
machine malfunctions.
Display resolution and sensi-
tivity, alarm annunciator priority, and
flashing indicator lights are adjust-
able too.
All these settings are second-
ary to the main job of carbon dioxide
control. They are explained else-
where.

HIGH/LOW CARBON DIOX-

IDE
Enter program mode. Navigate
to "rEV.d" function on level 3.
Control output is either direct
or reverse. Direct means carbon
dioxide decreases as output de-
creases on approach to set point.
Reverse means carbon dioxide in-
creases as output decreases on ap-
proach to set point.
To control high carbon dioxide
levels, set SP1 (setpoint 1) output
mode to reverse. To control low
carbon dioxide, set SP1 output mode
to direct. Mnemonic: "1r" or "1d".
Alarm SP2 output also sets but
is not active until alarm is configured.
Set "2r" or
"2d" later if
necessary.
 12
CALIBRATION ProCO2 model 120
version 1.0

Calibration is the key to control accuracy. Check calibration once

every two weeks to have as much confidence as necessary in
accuracy.
SENSORS
The sensor drives the controller.
If the sensor is not calibrated, it won't
be accurate. If the sensor is not
accurate, control won't be either.
Every sensor must be calibrated
both (1) initially when installed, new
or used, and (2) periodically over the
life of the sensor to compensate for
film blocking infrared part of sensor.
Sensors generate an electrical
signal (output) which is linear and
directly proportional with gas carbon
dioxide concentration:
CALIBRATION
Calibration electronically cor-
rects for the difference among sen-
sors. Makes raw output line super-
impose on calibrated line. Then all
sensors read accurately:
Calibration also compensates
for drift. Monitor drift with periodic
calibration checks. Recalibrate
when necessary:
CALIBRATION MENU
FUNCTIONS
"SPAn" and "ZEro" functions
on level 3 of the menu calibrate the
sensor. Both adjust sensor output
line without disrupting linearity, but
each works differently.
The "ZEro" function affects only
the Y-intercept of the line, not the
slope. It moves the line up and down
without changing the slope. Always
do the "ZEro" function first:
Increasing makes the y-intercept more
positive (moves the line up) -
Decreasing makes the y-intercept
more negative (moves the line down)

Although always linear and di-

rectly proportional, raw output can be
quite different for each sensor:
Sensor output also changes over
time (drift), generally in a slow down-
ward direction. Sensors remain linear
but gradually lose power:
The "SPAn" function adjusts
the slope of the line, but does not
affect the Y-intercept (set by "ZEro"
CALIBRATION function). Always do "SPAn" after
"ZEro":
STANDARDS
Sensors are calibrated to stan-
dard gas mixtures with known car-
bon dioxide concentrations. Since Raising increases slope:
sensor output is linear, calibrating at
two known points makes all other
points accurate as well.
One standard for calibration is
a compressed gas source of 10%
carbon dioxide and 90% oxygen. Lowering decreases slope:
Immerse the sensor in gas by using
a calibration chamber. Set the
"SPAn" to read "10" in work mode.
Compressed nitrogen can be
used as a standard. Immerse sen-
sor in control gas by using calibra-
tion chamber and bleed valve. Or, REMEMBER: never adjust
you could use room air as the sec- "ZEro" function without adjusting
ond standard. In both instances you "SPAn" function afterwards. "ZEro"
would set the "ZEro" to read "0.0". affects "SPAn" setting, but "SPAn"
Of course, other standards can does not affect "ZEro" setting.
be used as well. Or calibrate to third
party carbon dioxide analyzers. Just
be confident in your standard.
HOW TO USE
CALIBRATION CHAMBER
The calibration chamber is a
tool for immersing the sensor in cali-
bration gases. It connects to the gas
source via 1/16 in. ID tubing. It holds
the tip of the sensor in the gas
stream.
First, connect the calibration
chamber tubing to the "BLEED"
hose barb on the front panel of the
ProCO2.
Then insert the sensor tip into
the calibration chamber. The sensor
hole is sized for a snug fit and allows
the sensor to be in the gas stream
within the chamber:
Finally, open the bleed valve
knob on the front panel of the ProCO2
until you hear gas expelling slightly
through the calibration chamber:
CALIBRATION
PROCEDURE FOR
CARBON DIOXIDE
CONTROL
There are two ways to cali-
brate the "ZEro" setting. The first
way is to use room air as a stan-
dard and set the controller to
read "0.0", the second way is to
use compressed nitrogen as a
standard (BioSpherix recom-
mends to use nitrogen, as shown
in the following instructions).
1. Connect the 1/8 in. ID tubing
coming from the compressed nitro-
gen gas source to the "IN" barb on
the back of the ProCO2. Make sure
the gas is regulated 0-25 PSIG.
Never exceed 25 PSIG. Use ap-
proximately 2 PSIG for Culture Cham-
ber and use 15 PSIG for an Animal
Chamber.
2. Hook up calibration cham-
ber to "BLEED" hose barb on front
panel of ProCO2. Slightly open bleed
valve knob on front panel until you
can hear gas expelling slightly
through calibration chamber this will
establish a 0% carbon dioxide level
within the calibration chamber.
3. Insert sensor tip into calibra-
tion chamber and let sensor stabilize
in 0% carbon dioxide (minute or two).
Watch sensor response to immer-
sion in nitrogen in work mode to tell
when it stabilizes. Note whether
carbon dioxide reading is above or
below "0.0" and by how much.
4. Enter program mode by hold-
ing down the up and down buttons for
three seconds, navigate to"LEVL"
function and while holding down the
" * " button push the up button until
you reach "3". Then navigate to the
"ZEro" function and raise the setting
if reading was low, or decrease the
setting if reading was high.The num-
ber in the "ZEro" function stands for
the same amount in work mode. For
example: if you change the number
in "ZEro" function by ".2" the num-
ber in work mode will change by ".2".
Exit program mode to see new read-
ing by holding the up and down but-
tons for three seconds. Repeat until
reading is what it should be "0.0".
5. Close the bleed valve knob
then remove the calibration chamber
tubing from the "BLEED" barb. Turn
off the nitrogen at the compressed
gas source, open the bleed valve
knob again and allow the gas to
escape out the "BLEED" barb. When
the gas has all escaped close the
bleed valve knob. Then disconnect
the 1/8 tubing from the "IN" barb on
the back of the ProCO2.
13
ProCO2 model 120
version 1.0
6. Connect the carbon dioxide/
oxygen mix compressed gas with a
1/8 in. ID tubing to the "IN" barb on
the back of the ProCO2. The mix
should be a ratio of 10% (carbon
dioxide) - 90% (oxygen), check the
label on the compressed source for
exact percentage. Making sure the
gas is regulated to 0-25 PSIG. Never
exceed 25 PSIG. Connect the cali-
bration chamber tubing to the
"BLEED" barb on the front of the
ProCO2. Slightly open bleed valve
knob on the front panel until you can
hear gas expelling slightly from cali-
bration chamber this will establish a
10%-90% ratio of carbon dioxide-
oxygen mix within the calibration
chamber.
7. Insert the sensor tip into the
calibration chamber and let the sen-
sor stabilize in 10% carbon dioxide
(minute or two). Watch sensor re-
sponse in work mode to tell when it
has stabilized. Note whether carbon
dioxide reading is above or below
"10.0" and by how much.
8. Enter program mode,
change the "LEVL" to "3", navigate
to "SPAn" function, and raise the
setting if reading in work mode was
low, or decrease the setting if read-
ing in work mode was high. Exit
program mode to see new reading.
Repeat until reading is what it should
be "10.0". The number in "SPAn"
function isn't an exact corresponse
with the number in work mode, it is a
coarse adjustment.
Sensor is calibrated when it
reads 0% carbon dioxide after stabi-
lization in nitrogen and 10% carbon
dioxide after stabilization in carbon
dioxide/oxygen mixture.
When calibration is complete
close the bleed valve knob and dis-
connect the calibration chamber tub-
ing. Then turn off the compressed
gas supply and open the bleed valve
knob to expell the remaining gas.
Once the gas has all escaped, close
the bleed valve knob, and disconnect
the 1/8 in. tubing from the "IN" barb
on the back of the ProCO2. Now
connect the 100% carbon dioxide
gas to the "IN" barb, making sure the
regulator is set to 0-25 PSIG. Never
exceed 25 PSIG.

Tuning matches control parameters of ProCO2 to gas dynamics of
host chamber to achieve effective control.
TUNING
Different carbon dioxide control
jobs in different chambers require
specific control parameters. Tuning
means setting those control param-
eters that result in acceptable con-
trol.
Which parameters to set, and
how to set them, depends on the job.
Is oscillation acceptable? If so how
much? Is overshoot permissable?
Undershoot? How much? For how
long? Is the load fixed or variable?
Does setpoint change? Must recov-
ery be fast?
Capability exists to handle prac-
tically any carbon dioxide control job.
However, the more complex and de-
manding the job, the more involved
tuning becomes.
Tune only what's necessary to
accomplish the job. Any additional
control parameters probably won't
help, and might hurt. Excessive tun-
ing can reduce control stability.
Most carbon dioxide control jobs
are simple. Thus tuning is easy. Only
a few parameters need to be set.
Advanced control capabilities are avail-
able, but use them only if you need to.
Just watch the control process
and see how each control parameter
affects it. This is the only definitive
way. Usually it's the easiest too.
Tuning takes time. It takes gas.
It means sitting and watching the
control process, sometimes over and
over. There is no shortcut. Trial and
error is the only way to tell if a tune set
works. Along with the ProCO2 you are
provided with a "Control Parameters
Chart", this chart has all the pretuning
information that was done at the fac-
tory. Don't lose this chart, but if you
do then call the number at the front of
this manual to receive a new copy.
Always change only one control
parameter at a time. See how that
setting works first, before changing
another. Record each tune set. When
satisfied with control, copy and set
aside for safekeeping.
A tune doesn't have to be per-
fect to work. But don't stop short
either. Once tuning is done and done
TUNING
right, it may never need further at-
tention.
POWER
First adjust power. Power is
the only control parameter not set in
program mode. Once power is set,
other control parameters may be
tuned.
Power is a function of infusion
rate. The more gas infused per unit
time, the higher the power. Infusion
rate is adjustable up to 35 SCFH
(Standard Cubic Feet an Hour).
Infusion rate is a function of
gas supply delivery pressure. The
higher the pressure, the faster the
infusion rate. Gas supply delivery
pressure is set at the pressure regu-
lator on gas source. Infusion rate is
dependent on the pressure of the
gas coming from the compressed
gas source. Never exceed 25
PSIG.
ProCO2 power must roughly
match dynamic gas load of cham-
ber. Too little power and it takes
forever to reach set point. Too much
power and control is unstable.
The bigger the chamber, the
more power (gas) it takes. For
example, a small baby incubator
might take 2-5 SCFH , but a tissue
culture incubator might take 10-30
SCFH.
The leakier the chamber, the
more power (gas) it takes. For
example, an incubator with a tight
seal might take 10 SCFH, but one
with a loose seal might take 20
SCFH.
There are methods to calcu-
late power, but it's faster and easier
to adjust power empirically. Watch
the control process and adjust infu-
sion rate so ProCO2 is able to delib-
erately push carbon dioxide from
ambient to past set point under
normal load conditions.
Most jobs can be tuned over a
relatively wide power range. Once
the other control parameters are
tuned, however, any change in the
power/load balance may require re-
tuning those parameters.
14
ProCO2 model 120
version 1.0
STEPS:
1. Set gas supply delivery pressure.
Range 0-25 PSIG.
2. Watch the number in work mode
approach the set point. If too fast
and unstable, turn down pres-
sure to lower infusion rate. If too
slow, turn up pressure to
increase infusion rate. Never
exceed 25 PSIG.
ON/OFF CONTROL
Crude basic control. Suitable
when precise carbon dioxide levels
are not necessary.
Infusion stops when carbon di-
oxide reaches setpoint and resumes
only when carbon dioxide drifts back
past setpoint. Output is either 100%
or 0%. Oscillations are natural.
The only tune adjustment is
the dead band width. Dead band
width is distance between on-to-off
point and off-to-on point above and
below setpoint. Defines where no
switching occurs, but oscillation does
occur.
The larger the dead band width,
the larger the oscillation, but the less
switching on and off. Maximize the
dead band width to minimize wear
and tear on equipment. Set for larg-
est acceptable oscillation.
The smaller the dead band
width, the less oscillation, but the
more wear and tear.
On/off tuning has no provision
for fluctuating loads. Blindly responds
only to on-to-off point and off-to-on
point. Change in load may change
oscillation amplitude and/or period.
STEPS:
1. For on/off control, enter program
mode, navigate to "CYC.t"
function on level 1. Set "on.of".
Have to go down to "0" by
holding "*" button and pressing
down to get to "on.oF".
2. Then set dead band width. Index
down to "bAnd" function and set
distance between on-to-off point
and off-to-on point.
PROPORTIONAL CON-
TROL
Proportional control eliminates
oscillation. It controls steady-state
by weakening power as carbon diox-
ide level approaches setpoint. When
power (gas infused per unit time)
exactly matches load (gas leaking
out per unit time), carbon dioxide
stabilizes.
Unlike ON/OFF control where
power is either 100% or 0%, propor-
tional control can adjust power any-
where between 100% and 0%. Power
is adjusted by timing gas pulses.
"Proportional" means gas pulses get
proportionately smaller as the dis-
tance between carbon dioxide level
and setpoint (SP1) gets smaller.
Pulses are timed by setting a
cycle time and varied by percentage
of the cycle time. Maximum pulses
are 100% of cycle time. Minimum
pulses are 0% of cycle time. Propor-
tioned pulses are in between.
Timed pulse proportioning oc-
curs only over a narrow band of car-
bon dioxide levels centered around
set point. Control gas is infused full
blast (100% output) with no timing
until it reaches this band. Then it
cycles. The deeper it goes into the
proportional band, the shorter the
pulses. Band size determines how
quick pulses shorten and power thus
weakens.
Somewhere between 100%
and 0% of the cycle time there's a
pulse time that holds steady state.
However, if proportional band is too
small, it may not be easy to find.
Small changes in carbon dioxide will
cause huge changes in output, simi-
lar to ON/OFF control. Carbon diox-
ide will oscillate and never reach
steady state.
On the other hand, if propor-
tional band is too wide, proportioning
inhibits approach to setpoint. Power
starts decreasing way to soon.
Proportional band should be
big enough so there's no gross over-
shoot everytime proportioning kicks
in, but not so big that there's need-
less pulsing long before carbon diox-
ide level gets even close to setpoint.
Cycle time should be as long
as possible to minimize wear and
tear on equipment, but not so long
that it becomes unresponsive.
If no other control parameters
are set, proportional band centers
around setpoint SP1. All additional
parameters accentuate proportional
control. Without proportional control
( prop. band and cycle time), no other
parameters are active. Some affect
the position of the band, but not the
size. Some affect the size, but not
the position. None affect the cycle
time.
STEPS:
1. To tune proportional control, enter
program mode, navigate to
"CYC.t" function on level 1. Set
cycle time, anywhere from 0.1 -
81 seconds.
2. Then index down to "bAnd"
function and set proportional
band. Gas power is reduced, by
time proportioning action, across
this band centered around SP1.
3. Exit program mode to work mode
and watch control process. Note
whether process overshoots and
oscillates, or undershoots from
cycling long before reaching
setpoint.
4. Enter program mode, and
navigate to "bAnd" function
again. If process overshot and
oscillated, increase band size. If
process undershot and took long
to reach setpoint, decrease band
size.
5. Repeat steps 3-4 till steady state
is achieved, whether steady state
is at setpoint or not.
MANUAL OFFSET
Proportional band is basically
a "blind" control parameter. When
set alone, it positions itself arbitrarilly,
centered around the setpoint.
Chances are steady-state will not be
at setpoint.
If proportional control steady-
state is not at setpoint, the offset
must be eliminated to move steady-
state to setpoint.
Manual Offset moves propor-
15
ProCO2 model 120
version 1.0
tional band by a fixed distance so
that steady-state is at setpoint. If
steady-state is +0.3% carbon diox-
ide above setpoint, then an offset of
-0.3 will move proportional band down
so pulse is perfectly proportioned to
match load at setpoint.
Manual offset works good when
controlling against a fixed load. Once
set, it makes proportional control
nearly perfect. No overshoot either.
STEPS:
1. Watch control process in work
mode till steady state is reached.
Note the variance from setpoint.
2. Enter program mode, navigate to
"oFSt" function on level 1, and
set number that offsets variance.
NOTE: "int.t" function on level 1
must be set to "oFF" to manually
adjust offset, otherwise "oFSt"
function is not active but read-
only.
INTEGRAL CONTROL
AUTOMATIC OFFSET
RESET
Proportional control jobs, with
fluctuating loads require automatic
offset to keep steady state at
setpoint.
Integral control parameter au-
tomatically and continuously moni-
tors divergences between setpoint
and steady state, and acts to correct
offset by repositioning proportional
band. Changes the area between set
point line and carbon dioxide level
line, and shifts proportional band in
proper direction to minimize area.
As load changes, steady state fol-
lows setpoint if integral is tuned prop-
erly.
Integral control overshoots on
initial start up and after significant
interruptions. Far away from setpoint
it senses big offset, so it shoots
proportional band as far as possible
in corrective direction. Only after
passing setpoint does integral pick
up the need to bring proportional
band back to near setpoint.
Integral control parameter is
time. Resets offset as frequently as
you specify. If integral time is too
short, it recalculates too many times
before carbon dioxide moves much
and moves proportional band too far
too fast. Result is oscillation. Even-
tually oscillation will dampen to steady
state, if load hasn't changed yet.
If integral time is too long, it's
slow to respond. Steady state and
setpoint take a long time to merge.
STEPS:
1. Watch carbon dioxide control
process in work mode under
normal load change. Note offset
at one load. While load
changes, note time till new offset
stabilizes at steady state. To
change load open the chamber
door and ventilate.
2. Enter program mode, navigate to
"int.t" function on level 1, and
set integral time to 30-70% of
noted time. Range is 0.1 - 60
minutes.
3. Exit program mode to work mode.
Watch as normal load changes
occur, and note time till offsets
disappear.
4. Repeat steps 2 and 3 until control
is acceptable.
DERIVATIVE CONTROL
The most demanding control
job is the one that has to be fast and
tight under fluctuating loads. With
power and proportional band tuned
aggressively (high power, narrow
band), conditions are ripe for over-
shoot. Add integral action and over-
shoot is virtually guaranteed, at
startup and after large disturbances.
Derivative control suppresses
overshoot. It also speeds response
to disturbances, large or small.
Derivative algorithms measure
rate and direction of change of car-
bon dioxide in relation to setpoint.
Then temporarily but quickly shifts
proportional band in opposite direc-
tion, by a distance proportional to
the rate of change. The faster the
change, the further it shifts. Shift is
just long enough to "extract" an out-
put determination.
The effect is to quickly weaken
power on approach to setpoint, and
quickly boost power if carbon dioxide
suddenly pulls away from setpoint.
Properly tuned, derivative should not
disturb proportional or integral action
at steady state.
On fast approach to setpoint
without derivative, when carbon diox-
ide first hits proportional band the
initial pulse might be 97% of cycle
time. That's not very weak. The next 16
pulse might be significantly less, but ProCO2 model 120
by that time it's too late. Carbon
version 1.0
dioxide changed too fast. Overshoot 4. Re-enter program mode, and
already occurred. When integral navigate to "dEr.t" function
action finally kicks in, it's way too again. If too much overshoot on
late. approach and too slow response
With derivative, fast carbon di- to upset, decrease derivative
oxide change immediately pulls up time. If approach to setpoint is
inhibited and response under
proportional band. Then first pulse corrects, increase derivative
might be 1% of cycle time. This
time.
quickly slows approach to setpoint. 5. Repeat steps 3-4 until overshoot
Slower rate of change calculation on approach and response to
might only "extract" a pulse of 12% upset is acceptable.
of cycle time. Slower still, maybe
19%.
As rate of change of carbon
dioxide decreases, derivative action
decreases. At steady state there's
no derivative action. Ideally at that
point carbon dioxide is near setpoint
and integral action takes over.
Derivative also speeds re-
sponse to disturbances. Without it,
as carbon dioxide quickly diverges
away from setpoint, the first pulse
from proportional control band might
be only 22% of cycle time, the sec-
ond pulse 53%, the third 87%, and
by the time output goes to 100%,
carbon dioxide is far away.
With derivative, the fast change
in carbon dioxide away from setpoint
would immediately pull proportional
band back toward setpoint and result
in 100% output before carbon diox-
ide gets too far.
Derivative control is hardest to
tune. Proper setting is very sensitive
to gas dynamics and other control
parameters. If it is not required, keep
it off as it will tend to reduce control
stability.
Derivative parameter is time.
Sets rate of corrective action. Rule-
of-thumb: set 3-8 times faster than
integral. If too short it inhibits reach-
ing setpoint and slows response to
upsets. If too long, it oscillates and
overcorrects. Is only active in pro-
portional band.
STEPS:
1. With power, proportional, and
integral parameters set, watch
control process in work mode.
Note overshoot. Next open
chamber briefly to induce large
upset in control. Note speed of
response.
2. Enter program mode, navigate to
"dEr.t" function on level 1,and
set derivative time. Range 0.1 -
0.4 of integral time.
3. Exit program mode, air out
chamber, watch control process
and note overshoot on approach
and response to disturbance.

DERIVATIVE APPROACH
CONTROL
Derivative action (1) sup-
presses overshoot and (2) speeds
response to upsets. However, it may
not always be possible to do both
with one setting. Depending on the
power/load balance, sometimes de-
rivative tuned for quick response will
not suppress overshoot enough.
Derivative approach control
parameter "dAC" solves this prob-
lem. It tunes overshoot independent
of normal operating parameters by
controlling when derivative action
starts on approach to setpoint. Nor-
mally derivative action is only in pro-
portional band. "dAC" is a multiplier
of proportional band.
The smaller the "dAC" setting,
the closer to setpoint derivative ac-
tion begins. Too small and carbon
dioxide overshoots. Too large and
approach is slowed and stepped.
STEPS:
1. With power, proportional, and
integral parameters tuned, and
derivative time tuned for fast
response to upsets, watch
control process in work mode.
Note approach to setpoint.
2. Enter program mode and navi-
gate to "dAC" function on level 1.
If overshoot suppression is
needed, increase multiple of
proportional band. Range is
0.5 - 5.0. If approach is stepped
and slow, decrease multiple.
3. Repeat steps 1-2 until control is
acceptable.
2. Enter program mode and navigate
to "dEr.S" function on level 4. To 17
get to level 4 go to "VEr" on level ProCO2 model 120
3 and press both up and down
buttons and hold for 10 sec- version 1.0
onds. If response to upset is too
slow, decrease setting (limit is
0.1). If response is too fast
resulting in overcompensation,
increase setting (limit is 1.0).
3. Repeat steps 1-2 until control is
acceptable.
AUTOMATIC TUNING
If carbon dioxide setpoint is
sufficiently far away from outside air
level, ProCO2 can calculate it's own
control parameters. It runs a few
tests on host chamber. Takes a few
measurements. Calculates optimum
control parameters, and sets itself.
If setpoint is not far from ambi-
ent, autotuning will not work.
Autotuning sets all control pa-
rameters. Can't distinguish when
some are not needed. Thus some
jobs autotuned are overtuned.
Even jobs that are autotunable,
usually require some manual tuning
afterwards to fine tune.
Autotuning is most useful in
tough jobs that require complex tun-
ing. Gets you close fast (sometimes
very close). Thus saves on time and
gas.
Autotuning instructions will be
in the next version of this manual.

DERIVATIVE SENSITIVITY
This parameter compensates
when power/load balance is such
that derivative suppresses overshoot
but does not sufficiently speed up
response to upsets.
Derivative sensitivity is frac-
tion multiplier of derivative time.
Range is 0.1 - 1.0. By fractioning
derivative time it speeds action up to
10 times faster.
STEPS:
1. With power, proportional, and
integral parameters tuned, and
derivative time tuned for over-
shoot suppression, watch
control process in work mode,
introduce upset by opening
chamber door(s), and note
speed of response to upset.
 18
Single Setpoint Control ProCO2 model 120
version 1.0

This section will describe how to use single setpoint control with the
ProCO2. Single setpoint control is a way to control the level of gas in
the chamber. For more information, see the “Operation” section of this
manual. The ProCO2 can only use 0-20 for a set point. Other wise the
sensor will overload.To set a setpoint on the controller, follow these
steps:

1. Make sure the compressed carbon dioxide gas supply is connected to the back panel of
the ProCo2 at the “IN” barb.
2. Open the regulator on the compressed carbon dioxide source to 0-25 PSIG, Never
exceed 25 PSIG or damage will occur to the unit. For the best results use approxi-
mately 2 PSIG in a Culture Chamber and 15 PSIG in an Animal Chamber when operating.
3. Check to make sure that the gas is connected properly by opening the bleed valve on
the front panel. If gas is heard expelling from the “BLEED” barb then it is connected cor-
rectly. Once you have confirmed that the gas is connected properly, close the bleed valve.
4. To set a setpoint, push and hold the “*” button. While holding the “*” button use the up
and down buttons to adjust the “set” to read what you want the setpoint to be. Once you
have adjusted the number to the desired reading, release the “*” button and the controller
will begin to go towards the new setpoint. Remember you can only use 0-20 for a setpoint
anything higher will overload the sensor.
5. Flip the switch on the front panel labeled “GAS” upward. This will allow the gas to enter
the chamber.
 19
Setting the Alarm Setpoint ProCO2 model 120
version 1.0

This section will explain how to set the alarm setpoint. Remember,
when you want the alarm function to be active you must make sure the
alarm switch on the front of the controller is on.

1. Push the up and down buttons at the same time for 3 seconds.
2. Now, go to “LEVL 2”. In ‘LEVL 2” navigate to “SP2.A”. Change this number to either “DV.hi”
(Deviation High) or “DV.Lo” (Deviation Low). This will determine whether the alarm activates
below or above the “SEt.2” (alarm setpoint).
3. In “LEVL 1” navigate to “SEt.2”. Adjust this number by holding the “*” button and using the up
or down buttons. Change this number to the desired alarm setpoint.
4. In “LEVL 1” navigate to “bnd.2”. This number will determine the range of the alarm setpoint.
The “bnd.2” number is a coarse adjustment, the number that you change this to will
not be the exact size of the band width. To set the alarm to activate as quickly as possible,
set the band to “0.1”. Increase the number to increase the range. Remember it is a coarse
adjustment.
5.Remember, if you want the alarm to have the ability to come on you must flip the “Alarm”
switch on the front panel.
 20
Maintenance ProCO2 model 120
version 1.0

This section will describe how to check calibrate, recalibrate and how to
replace the sensor.

Check Calibration and Re-calibration

The sensor’s calibration will need to be checked at least once every two weeks. To
check the calibration you have to disconnect the sensor from the chamber. You will need
the calibration chamber and 1/16 in. tubing to check the calibration. Follow steps 1-3 of
“Calibration Procedure for Carbon Dioxide Control” to check zero calibration and follow
steps 5-7“Calibration Procedure for Carbon Dioxide Control” for SPAN calibration. If it isn’t
accurate then it will need to be recalibrated. To recalibrate the sensor follow the full set of
procedures as was used for the original calibration. It may not require calibrating, but over
time dust will accumulate on the infrared part of the sensor and it will need to be
recalibrated to compensate. The sensor should last indefinitely.

New Sensor

In the event that the sensor quits functioning call the number on the side of the sensor
to purchase a new one. To replace the sensor follow step “6” of “ProCO2 Installation”.
 21
WARRANTY ProCO2 model 120
version 1.0

BIOSPHERIX, LTD.
ONE-YEAR WARRANTY
Except to the extent specifically stipulated, BioSpherix, Ltd. shall have no
liability or obligation under warranty, express or implied, including the implied
warranty of merchantability and any implied warranty of fitness for a particular
purpose; statutory or otherwise except as stated.

BioSpherix, Ltd. (BioSpherix) warrants that all apparatus of its manufacture has
been factory inspected and is free from defects in material and workmanship. And that
when such apparatus receives normal use and service, BioSpherix will correct defects
in material and workmanship that might occur within a twelve (12) month period from
the date of delivery to customer. This warranty is limited to the repair, replacement or
exchange of parts, subject to the exceptions listed below, which prove defective on
examination by BioSpherix. Costs assumed by BioSpherix under this warranty cover
only the cost of material and workmanship. This warranty does not cover sensors.

GENERAL CONDITIONS OF WARRANTY

This warranty shall be void if apparatus, in the judgement of BioSpherix, has been
subject to mis-use, negligence, chemical action, accident or operated contrary to those
operating procedures recommended by BioSpherix, or if the serial number and/or trade-
marks have been altered, defaced or removed.
BioSpherix shall not be liable for any delay in performance under this warranty
caused by any contingency beyond the control of BioSpherix, including War, govern-
ment restriction or restraints, strikes, acts of God, or short or reduced supply of raw
materials.
Costs borne by BioSpherix do not reflect the cost of labor involved; nor will
BioSpherix assume the responsibility for payment of same except when expressly
consented to in writing.
Determination of whether or not apparatus has been used properly or improperly
(thereby voiding any warranty) is solely at the discretion of BioSpherix.
BioSpherix reserves the right to inspect any and all equipment or parts of said
equipment claimed to be defective prior to authorizing warranty repairs.
All apparatus claimed to be defective and sent to BioSpherix for repairs must be
returned freight prepaid.
BioSpherix disclaims all liability to its customers, dealers and all others concerned
for special or consequential damages arising out of any circumstances or incident
whatsoever, connected with the use, operation, manufacture, sale, handling, repair,
maintenance, replacement or any other circumstance connected with the use of said
apparatus.
BioSpherix, Ltd.
Lacona, New York, USA
 Adapting a Generic Chamber 
Adapting a Ge-

Manual
version 0.2 May 2006
neric Chamber
version 0.2

This manual will describe how to install and adapt a generic chamber.
Read this manual thoroughly before installing and adapting.

PO Box 87
19 Demott St.
Lacona, New York 13083
PHONE 315-387-3414
FAX 315-387-3415
TOLL FREE US/CAN 800-441-3414
www.biospherix.com

Anyone who has not thoroughly read and understood

! this manual, must never attempt to operate the

equipment !
 Table of Contents Adapting a Ge-


neric Chamber
version 0.2

Safety Instructions.........................................................................................3

Installation Overview.....................................................................................4

Adapting a Chamber for a ProCO2................................................................5

Mounting an Adapter Plate to the Chamber for a ProCO2...........................6

Adapting a Chamber for a Proox 110............................................................7

Mounting an Adapter Plate to the Chamber for a Proox 110......................8

Adapting a Chamber for a Proox 360...........................................................9

Mounting an Adapter Plate to the Chamber for a Proox 360....................10

Mounting a Pod to the Chamber for a Proox C21......................................11

 
Adapting a Ge-
neric Chamber
version 0.2
SAFETY INSTRUCTIONS

POWER CORD PROTECTION power supply cords should be routed so that they are not
likely to be walked on or pinched by items placed upon or against them.
ELECTRIC SHOCK if modifying host chamber, be careful not to drill or cut into electrical
wires hidden behind chamber wall. Never drill or cut blindly.
LOW OXYGEN ATMOSPHERES never enter a chamber which has a low oxygen atmo-
sphere because of severe danger of suffocation. Host chamber should be in well ventilated
room. Control gas (nitrogen or other low oxygen gas) continuously leaks out of chamber
and should never be allowed to build up in room outside of chamber.
HIGH OXYGEN ATMOSPHERES never enter a chamber which has a high oxygen at-
mosphere due to danger of oxygen toxicity. Never smoke or allow any source of fire or
spark in or around a chamber with high oxygen atmosphere. Oxygen radically promotes
combustion and can be explosive. Host chamber should always be in well ventilated room.
Oxygen continuously leaks out of chamber and should never be allowed to build up in room
outside chamber.
VENTILATION unit should be situated so that its’ location or position does not interfere with
proper ventilation. Host chamber should never be in a poorly ventilated area.
HEAT host chamber should be situated away from heat sources such as radiators, heat
registers, stoves, or other appliances or processes that produce heat.
HIGH CONCETRATION OF GAS never adapt host chamber when gas is evident inside of
chamber.
DRILLING PRECAUTIONS keep a firm grasp on the drill at all times, drill slowly and cau-
tiously. Beware of sharp drill bit.
MAKING CUTOUT FOR ADAPTER PLATE the cutout for the adapter plate should only be
made by an experience fabricator.
 Installation Overview 
Adapting a Ge-
neric Chamber
version 0.2

If the chamber being used is not one from BioSpherix then follow these
guidelines to adapt the chamber for a BioSpherix unit.

The chamber must be in a well ventilated room. Control gas will continually leak out of
chamber and should never be allowed to build up outside of chamber.
The chamber must be semi-sealed. If the chamber is sealed too tight then it may de-
velop positive pressure which sensors can’t tolerate. Positive pressure is when there is no
small opening for the gases inside the chamber to vent out or for the outside air to vent in.
The chamber can’t be too leaky or you won’t be able to hold an inner atmosphere inside of
the chamber.
It is important to have leaks when you are doing an experiment that deals with both
low levels of oxygen and high levels of oxygen. With a low level oxygen experiment you
will be infusing nitrogen (or an oxygen poor gas), which will bring the level of oxygen down,
and through the leak(s) the outside air will be bringing up the level of oxygen. This is im-
portant because without the outside air coming in, the oxygen level would drop to 0% and
stay there. With a high level of oxygen experiment, you will be infusing oxygen (or an
oxygen rich gas), which will bring the level of oxygen in the chamber up, and through the
leak(s) the outside air will be bringing down the level of oxygen, because the outside air is
made up of only 20.8% oxygen. This is important because without the outside air coming
inside the chamber the oxygen level would rise to 100% and stay there.
Plan the best access point(s) into the chamber for the sensor and the infusion tube.
Any passageway will work as long as the sensor can monitor the inside of the chamber and
gas can be infused into the chamber.
There are three ways to install the sensor and infusion tubing: either on the
chamber, in the chamber or by mounting a BioSpherix adapter plate to the cham-
ber (see the appropriate unit’s section for information on how to adapt the chamber three
ways). Along with the instrument you will also receive a muffler, which can be attached to
the end of the infusion tube if you wish.

Muffler
 Adapting a Chamber for a ProCO2 Adapting a Ge-


neric Chamber
version 0.2

There are three ways to adapt the generic chamber for a ProCO2. One
way is having the sensor inside of the chamber and routing the cables
through an access port. The second way is to drill a hole in the cham-
ber to accomodate a port that is supplied by BioSpherix. The third
way is to make a cutout on the chamber that will accomodate a Bio-
Spherix adapter plate.
Inside of Chamber:
The first way to install the sensor is inside of the chamber. You will have to drill a hole
(specific size, below) so that the sensor cable and the infusion tube can both fit through.
Once the hole is drilled you can slide the sensor cable then the infusion tube through the
opening. Then, you can connect the sensor to the sensor cable inside of the chamber and
attach the muffler to the infusion tube. By installing the sensor in this manner you have cre-
ated a small leak for the gas inside to vent out and the outside air to vent in. You may need
more of a leak depending on the size of the chamber.
IMPORTANT: If you place the sensor inside the chamber it must be placed in such a
way so that it can be pulled out of the chamber for calibration purposes. In order to cali-
brate the sensor it has to be outside the chamber in the outside air.

Placing the Sensor Inside of the Chamber:

.
1. Drill an opening into the chamber, approximately 5/8in. for the sensor cable and infusion
tubing. With the sensor cable and infusion tube inside of the chamber, make sure to attach
the sensor to the sensor cable and attach the muffler to the infusion tube.
Mounting the Sensor on the Chamber:
Remember: If you are using your own bulkhead, use your own specific measure-
ments for drilling an opening to properly fit the bulkhead.
The second way to install the sensor is to mount it on the chamber. You will have to
drill a hole (specific size, below) so that the bulkhead (provided) snugly fits in the open-
ing. Once you have drilled the hole simply place the bulkhead in and tighten down with
nut. Then mount the sensor into the bulkhead. This will allow the sensor to be mounted
outside of the chamber, but still monitor the inner atmosphere. You will also need a sepa-
rate hole(specific size, below) for the muffler to fit through so that gas can be infused into
the chamber. If you use this manner then make sure there is a small opening for the gas to
leak slightly so as not to create positive pressure. The size of the hole is dependent upon
how big your chamber is, the bigger the chamber the bigger the opening, the smaller the
chamber the smaller the opening. The chamber may be leaky enough without an opening.

Mounting the Sensor on the Chamber:

1. Drill an opening into the chamber approximately 1in. for the bulkhead and a separate
5/16in. hole for the infusion tube. Attach the muffler to the infusion tube, once the tube is
inside of the chamber.
 Mounting an Adapter Plate 
Adapting a Ge-
to the Chamber for a ProCO2 neric Chamber
version 0.2

Use the following drawing to determine the correct size of the holes and
the cutout size you will need to fit the BioSpherix adapter plate to the
chamber. You may not need to use the supplied muffler if you are
adapting the chamber in this manner.
 Adapting a Chamber for a Proox 110 
Adapting a Ge-
neric Chamber
version 0.2
There are three ways to adapt the generic chamber for a Proox 110. One
way is having the sensor inside of the chamber and routing the cables
through an access port. The second way is to drill a hole in the cham-
ber to accomodate a port that is supplied by BioSpherix. The third
way is to make a cutout on the chamber that will accomodate a Bio-
Spherix adapter plate.
Inside of Chamber:
The first way to install the sensor is inside of the chamber. You will have to drill a hole
(specific size, below) so that the sensor cable and the infusion tube can both fit through.
Once the hole is drilled you can slide the sensor cable then the infusion tube through the
opening. Then, you can connect the sensor to the sensor cable inside of the chamber and
attach the muffler to the infusion tube. By installing the sensor in this manner you have cre-
ated a small leak for the gas inside to vent out and the outside air to vent in. You may need
more of a leak depending on the size of the chamber.
IMPORTANT: If you place the sensor inside the chamber it must be placed in such a
way so that it can be pulled out of the chamber for calibration purposes. In order to cali-
brate the sensor it has to be outside the chamber in the outside air.

Placing Sensor Inside of Chamber:

1. Drill an opening into the chamber, approximately 9/16in. for the sensor cable and infu-
sion tubing of 1/8in. ID. Once the infusion tube is routed into the chamber attach the muf-
fler. From inside of the chamber route the smaller end of the sensor cable outside of the
chamber and to the unit. Make sure to connect the sensor to the sensor cable inside of the
chamber.
Mounted on Chamber with Supplied Bulkhead:
Remember: If you are using your own bulkhead, use your own specific measure-
ments for drilling an opening to properly fit the bulkhead.
The second way to install the sensor is to mount it on the chamber. You will have to
drill a hole (specific size, below) so that the bulkhead (provided) snugly fits in the open-
ing. Once you have drilled the hole simply place the bulkhead in and tighten down with nut.
Then mount the sensor into the bulkhead. This will allow the sensor to be mounted outside
of the chamber, but still monitor the inner atmosphere. You will also need a separate hole
(specific size, below) for the muffler to fit through so that gas can be infused into the cham-
ber. If you use this manner then make sure there is a small opening for the gas to leak
slightly so as not to create positive pressure. The size of the hole is dependent upon how
big your chamber is, the bigger the chamber the bigger the opening, the smaller the cham-
ber the smaller the opening. The chamber may be leaky enough without an opening.

Mounting the Sensor on the Chamber:

1. Drill an opening approximately 3/4in. for the bulkhead and a separate 5/16in. hole for the
infusion tubing of 1/8in. ID. Route the infusion tube through the hole and place the muffler
on the end of the tube.
 Mounting an Adapter Plate 
Adapting a Ge-
to the Chamber for a Proox 110 neric Chamber
version 0.2

Use the following drawing to determine the correct size of the holes and
the cutout size you will need to fit the BioSpherix adapter plate to the
chamber. You may not need to use the supplied muffler if you are
adapting the chamber in this manner.
 Adapting a Chamber for a Proox 360 Adapting a Ge-


neric Chamber
version 0.2
There are three ways to adapt the generic chamber for a Proox 360. One
way is having the sensor inside of the chamber and routing the cables
through an access port. The second way is to drill a hole in the cham-
ber to accomodate a port that is supplied by BioSpherix. The third
way is to make a cutout on the chamber that will accomodate a Bio-
Spherix adapter plate.
Inside of Chamber:
The first way to install the sensor is inside of the chamber. You will have to drill a hole
(specific size, below) so that the sensor cable and the infusion tube can both fit through.
Once the hole is drilled you can slide the sensor cable then the infusion tube through the
opening. Then, you can connect the sensor to the sensor cable inside of the chamber and
attach the muffler to the infusion tube. By installing the sensor in this manner you have cre-
ated a small leak for the gas inside to vent out and the outside air to vent in. You may need
more of a leak depending on the size of the chamber.
IMPORTANT: If you place the sensor inside the chamber it must be placed in such a
way so that it can be pulled out of the chamber for calibration purposes. In order to cali-
brate the sensor it has to be outside the chamber in the outside air.

Placing Sensor Inside of Chamber:

1. Attach the provided gas fitting to the 1/4 in. ID infusion tubing. Attach the 1/8 in. ID
tubing to the other end of this gas fitting. Now, drill an opening into the chamber, approxi-
mately 9/16in. for the sensor cable and infusion tubing (which is now 1/8 in. ID). Once the
infusion tube is routed into the chamber attach the muffler. From inside of the chamber
route the smaller end of the sensor cable to outside of the chamber and to the unit. Make
sure to connect the sensor to the sensor cable inside of the chamber.
Mounted on Chamber with Supplied Bulkhead:
The second way to install the sensor is to mount it on the chamber. You will have to
drill a hole (specific size, below) so that the bulkhead (provided) snugly fits in the open-
ing. Once you have drilled the hole simply place the bulkhead in and tighten down with
nut. Then mount the sensor into the bulkhead. This will allow the sensor to be mounted
outside of the chamber, but still monitor the inner atmosphere. You will also need a sepa-
rate hole(specific size, below) for the muffler to fit through so that gas can be infused into
the chamber. If you use this manner then make sure there is a small opening for the gas to
leak slightly so as not to create positive pressure. The size of the hole is dependent upon
how big your chamber is, the bigger the chamber the bigger the opening, the smaller the
chamber the smaller the opening. The chamber may be leaky enough without an opening.

On the Chamber with Oxygen Sensor:

Remember: If you are using your own bulkhead, use your own specific measure-
ments for drilling an opening to properly fit the bulkhead.
1. Attach the provided gas fitting to the 1/4 in. ID infusion tube. Attach the 1/8in. ID
tubing to the other end of this gas fitting. Now, drill an opening approximately 3/4in. for the
bulkhead and a separate 5/16in. hole for the infusion tube (which is now 1/8 in. ID.). Once
the infusion tube is routed into the chamber attach the muffler.
 Mounting an Adapter Plate 10
Adapting a Ge-
to the Chamber for a Proox 360 neric Chamber
version 0.2

Use the following drawing to determine the correct size of the holes and
the cutout size you will need to fit the BioSpherix adapter plate to the
chamber. You may not need to use the supplied muffler if you are
adapting the chamber in this manner.
 Mounting a Pod 11
Adapting a Ge-
to the Chamber for a Proox C21 neric Chamber
version 0.2

Use the following drawing to determine the correct size of the holes and
the cutout size you will need to fit the BioSpherix Proox Model C21’s
Pod to the chamber.