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Antimicrobial, Disinfectant, Biocide, Sterilant, Biostatic and Odor

Eliminator

It is a proven technology to inhibit the growth of fungi and bacteria.

Is based on advanced silane chemistry : the organo silane technology is
proven to be very effective, following 30 years of development.
Traditional antimicrobials provide short-term kill of microorganisms.
Unlike disinfectants, sanitizers and bleaches, the water based
Saniguard-7500 forms a molecular bond to surface. The molecule
disrupts the cell membranes of harmful fungi and bacteria, inhibiting,
reducing and providing long-term, residual kill.

It can produce a durable, non-migrating antimicrobial finish to a wide

range of textiles. SANIGUARD-7500 can form an invisible and durable
micro-biostatic coating on a broad range of hard surfaces.

The physical, rather than chemical, mode of action of SANIGUARD-7500

does not affect normal skin flora, does not lose strength with use and
does not promote adaptative organisms (super bugs)

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 SANIGUARD-7500 : Mode of action

Part I : The first part of the molecule is the silane base. This silane base is the
antimicrobial anchor. The antimicrobial is anchored by covalent bonds which
are formed gradually through hydrolysis reactions that bond the antimicrobial
permanently to almost any surfaces and allow for crosslinking and
polymerisation with other molecules.

Part II : The second part of the molecule is the centrally located positively
charged nitrogen. It plays an important role in the active nature of the
antimicrobial. Some membranes of microbes are negatively charged; when in
close proximity, the microbes are drawn into the active surface of the
antimicrobial and

Part III : The third segment is the edge that leads to blow in the offending
microbes. This long molecular chain acts like a sword that punctures the
cell membranes of all microbes coming in contact with it.

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When a microorganism comes in contact with
the treated surface, the C-18 molecular sword
punctures the cell membrane and the electrical
charge shocks the cell. As the microbe is further
drawn into the antimicrobial, it is electrocuted

Since nothing is transferred to the now dead cell, the

antimicrobial doesn’t lose strength and the sword is
ready for the next cell to contact it. The active layer
remains intact, efficacious and ready for the next cell
that approaches.

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Summary
When the antimicrobial is bonded to the surface of an object, it is ready for
defense.

As the microbes come into contact with the “antimicrobial” surface, they
are first punctured by the long molecular chain.
The “positive-negative” attraction in between the positively charged
nitrogen and the negative cell membrane draw the microorganism even
further down on the sword.
This modified cell can end the life of a microbe but the end point is the
contact between the negative and the positive charges : the cell membrane
is electrocuted and blown apart delivering inescapable kill

In addition, since this process transfers nothing from the antimicrobial to

the cell, the antimicrobial stands ready for the next “wave” and can
continue to work at full strength and definitely.
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Applied in a single stage of the wet finish process, the
attachment of SANIGUARD-7500 to surfaces involves two
means.

First and the most important is a very rapid process, which

coats the substrate (fabric, fiber, etc), with the cationic
species (physics sorption) one molecule deep. This is an ion
exchange process by which the cation of the silane
quaternary ammonium compound replaces protons from
water or chemicals on the surface.

The second mechanism is unique to materials such as silane

quaternary ammonium compounds. In this case, the silanol
allows for covalent bonding to receptive surfaces to occur
(chemisorptions). This bonding is then made even more
durable by the silanol functionality, which enables them to
homopolymerize. After they have coated the surface in this
manner, they become virtually irremovable, even on
surfaces with which they cannot react covalently.

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1. Molecule used in SANIGUARD-7500

2. Surface view

3. Treated surface

We have 2 reactions:
1. The product forms a molecular BOND to any fabric
2. Product forms a film : crosslinking of the product

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Process of Unpleasant Body Smell Generation

Human Sweating Bacterial Foul smell

body Fat secretion Contamination Contamination

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Sweating
Spiritual sweating/strain Sensory excitation
Thermal sweating/Exercise Thermal excitation

Smell of sweat
Seat under the arm Initially No Smell
After six hours Foul Smell

Storage the swat under sterile condition

After two weeks No Smell

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 Relationship Between Fiber and
Micro-organism
Dyeing Factory After dye treatment (while keeping)

Coloring, discoloration, deterioration in physical properties

Clothing Wearing Bad odor

Keeping Growth of mildew

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Parts of body Bacteria number (colony forming unit/cm2)
Head 1,500,000
Armpit 2,400,000
Forearm 450
Back 310
Forehead 200,000

Total surface area of skin for an adult Approx. 1.6m2

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Subject Left Foot/Right Foot Bacterial Number (C.F.U./cm2)
Toe Heel Instep
A Left 4.7x10 5 3.4x10 5 8.9x102
Right 5.4x105 1.7x105 4.6x102
B Left 5.8x106 3.9x106 3.0x103
Right 1.7x106 4.3x106 2.4x103
C Left 5.3x106 2.1x106 4.3x103
Right 5.2x106 9.4x106 4.8x103
D Left 9.2x106 4.7x106 3.0x103
Right 8.1x106 4.2x106 1.3x104
E Left 3.9x106 1.6x106 9.8x102
Right 5.1x106 2.3x106 2.1x103
F Left 4.3x105 2.7x105 6.6x103
Right 3.0x106 1.7x106 6.3x103
G Left 9.7x105 4.3x105 4.2x104
Right 2.6x106 3.7x106 2.1x104
----
 3.7x106 2.2x106 7.9x103

Used socks Cotton 100%

Wearing Time 9:00 ~ 17:30
Specimen Size 3cm x 3cm (Cut after wearing)

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 Classification Application
Textiles Socks, underwear, dress-shirt, working uniform, formal dress
Bedding & Night clothes (filling of a quilt, quilt cover, sheet,
pajamas, blanket)
Gloves, hat, handkerchief.

Household goods Kitchen goods (towel, dish cloth, chopping board, water
purifier, sponge)
Bath room & toilet goods (bathtub, washbowl, bath mat,
toothbrush, toilet fixtures, toilet seat cover)

Footwear Shoes, Slippers, shoe insole.

Interior goods Carpet, curtain, upholstery.

Home electric appliances Vacuum cleaner, air cleaner, washing machine, telephone, air
conditioner, headphones, floppy disk.
Others Stationary (Ball-point pen, file-cover, folder).
Cards (telephone card, cash card).
Watch-strap, belt.

Hospitals Bedsheets, surgical nonwoven fabrics, wet wipes,

filters(Filtration media like AC), hospital floorings, walls,
operation theatre, ICU units, Mattress Pad
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Remove bodily secretions by washing Bathing

Prevent the growth of bacteria Antimicrobial Agents

Reduce sweating Antiperspirant

Deodorize by chemical reaction Deodorant

Mask with perfume Aromatics/perfume, etc.

Adsorb with active carbon Active carbon

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Which Goods Do You Want to be Treated By Antimicrobial Agents ?
The survey was conducted on 1,600 men and women from 20 to 59 year-old.

Socks 28.0%
Underwear 13.3%
Underpants 6.0%
Handkerchiefs 5.6%
Towels 5.3%
Shoes 4.9%
Hand straps of train 4.2%
Slippers 3.4%
Telephone 2.5%
Pantyhose 2.3%
Others
Toilet seat
Chopping Board
Wallet
Hand towels at coffee shop
Remote unit
Bedsheets

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Antimicrobial Finish Socks In Japan
Classification Production Number Antimicrobial finish number
Man’s Socks 400 million/pair 200 million/pair (50%)
Lady’s Socks 300 million 10 million (3%)
Child’s Socks 200 million 20 million (10%)
Stocking 1,300 million 100 million (8%)
Others 100 million 10 million (10%)
Total 2.3 billion/year 340 million/year (15%)

Feature of Antimicrobial Agents for Textile

Classification Treatment Method Durability Safety (LD50) Leach/Non-
leach
Saniguard 7500 Dilute with water Excellent 12,270mg/kg Non-leach
Quaternary Mix with reactive resin Fair 300`400 mg/kg Leach
Ammonium Compounds for fiber
Biguanide Mix with reactive resin
for fiber
Chitin, Chitosan Mix with reactive resin Fair >5,000mg/kg Leach
for fiber
Inorganic Silver Mix with raw materials Excellent >2,000mg/kg Slow-release
of synthetic fiber
Natural extract Microcapsule Good >2,000mg/kg Slow-release

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 100
90

PERCENT RETENTION OF14C-

80
70 cellulose

SANIGARD-7500
60 acetate
cotton
50 polyester
40
30
20
10
0
1 2 3 4 5 6 7 8 9 10
NUMBER OF WASHES

Effect of repeated washings upon the surface retention of

14C-labeled 3-(trimethoxysilyl) propyldimethyloctadecyl

ammonium chloride on secondary cellulose acetate (A),

cotton (B), and polyester (C).

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Micro-oganisms susceptible to Saniguard-7500 Antimicrobial Agent
Bacteria (gram positive)
 Streptococcus aureus
 Streptococcus faecalis
 Bacillus subtilis
Bacteria (gram negative)
 Samonella typhosa
 Samonella chloeraesius
 Escherichia coli
 Mycobacterium tuberculosis
 Pseudomonas aeruginosa
 Aerobacter aerogenes
Yeast
 Saccharomyces cerevisiae
 Candida albicans
Algae
 Cyanophyta (blue-green)oscillatoria
 Cyanophyta (blue-green) anabaena
 Chrysophyta (brown)
 Chlorophyta (green) Selenastrum gracile
 Chlorophyta (green)Protococcus
Fungi
 Aspergillus niger
 Aspergillus terreus
 Chaetonium globosum
 Aspergillus flarres
 Aspergillus verrucaria
 Pencillum funiculosm
 Pullularia pullulans
 Cephaldascus fragans
 Trichoderm sp. Madison P-42
 Trichophyton interdigital
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Dilute with water

Treatment concentration :Depend on materials, treatment

method, usage, etc.

Treatment Machine

• Yarn
o Obermaier package dyeing
machine
o Hank dyeing machine
o Cheese dyeing machine
• Textile fabric
o Winch dyeing machine
o Pad-roll dyeing machine
• Finished Good
o Drum dyeing machine

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Antimicrobial Activity Evaluation Methods of SANIGUARD – 7500

1. MIC
2. AATCC Test Method 30
3. AATCC Test Method 90 (Halo Test)
4. AATCC Test Method 100
5. AATCC Test Method 100, Modified
6. Shake Flask Test Method
7. AATCC Test Method 147

American Association of Textile Chemists and Colorists

Test Method 30 (AATCC Test Method 30)

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Minimum Inhibitory Concentration Test
Saniguard 7500 Antimicrobial Agent

Test Organism MIC(mg/ml.)

Streptococcus faecalis Gram(+) Bacteria 10
Escherichia coli Gram(-)Bacteria 100
Pseudomonas aeruginosa Gram(-) Bacteria 100
Aspergillus niger Fungus 1000

American Association of Textile Chemists and Colorists

Test Method 90 (AATCC Test Method 90)

Halo Test (Bactericidal Test)

Inoculum is mixed with nutrient agar media

Pour into sterilized petri dish and make agar plates
Put specimen on the center of the plates
Incubate at 37ºC
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High concentration
Leaching concentration
Limit point of bactericidal
concentration

Live bacteria
Low concentration

Dead bacteria

Near Distance from the sample Far

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 AGAR PLATE MIXED INOCULUM
Treated sample

Antimicrobial agent treated sample is spread into Agar plate (leaching)

Halo Test

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American Association of Textile Chemists and Colorists Test Method 100
(AATCC Test Method 100)

RESULTS

American Association of Textile Chemists And Colorists Method 100,

Antimicrobials on Fabrics

SANIGUARD 7500 Antimicrobial Agent Treated Non-wovens

Sample Micro-organism Percent Reduction

Control Staphylococcus aureus 16

Treated Gram(+) Bacteria 100
Control Escherichia coli 0
Treated Gram(-) Bacteria 99.6
Control Klebsiella pneumoniae 0
Treated Gram(-) Bacteria 100
Control Saccharomyces cerevisiae 0
Treated Yeast 99.9
Octyl phenol 10 moles surfactant used.

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Notes and References

The dilution of the test organism may be prepared in 0.85%

saline solution if a steady- state culture is desired is desired
during the contact period with a fabric.

A surfactant may be added to the dilution medium to cause a

reduction in bacterial numbers, by prior testing at the intended
use concentration.

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Page 26
 Reduction rate (%)
= (Test bacteria no. before shaking)–(Test bacteria no. before shaking)x100(%)
(Test bacteria number before shaking)

Features of Shake Flask Test Method

Can reduce personal error.

Can observe a change on standing using the same specimen,
Can evaluate even hydrophobic fiber.
No effect of neutralizer.
Can evaluate powder, plastic, etc.

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Sample Microorganism Percent Reduction
Untreated Citrobacter diversus 14.3
Treated Wound Isolate 93.6
Inoculum 0
Untreated Pseudomonus aeruginosa 28.3
Treated Urine Isolate 99.9
Inoculum 0
Untreated Staphylococcus aureus 0
Treated Wound Isolate 99.7
Inoculum 0
Untreated Escherichia coli 11.6
Treated Urin Isolate 98.6
Inoculum 0
Untreated Proteus mirabilis 0
Treated Wound Isolate 99.5
Inoculum 0
Sontara Fabric
Shake Flask Test,

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 Fiber Inoculum Fiber Sanigard-7500 treatment
Inocumum
Solved agent
Survived bacteria
Death bacteria
Death bacteria

(a) Water soluble type (b) Water non-soluble type [Sanigard-7500]

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ATCC Test Method 147 : Antibacterial Activity Assessment of Textile
Materials: Parallel Streak Method
AATCC 147 is a Qualitative test used to detect bacteriostatic activity on
textile materials.

The test Method determines antibacterial activity of diffusable

antimicrobial agents on treated textile materials.

The test is followed in the cases where the antibacterial agent is

migratory i.e diffusible through the agar.

The size of the zone of inhibition and the narrowing of the streaks
caused by the presence of the antibacterial agent permit an estimate of
the residual antibacterial activity after multiple washings.

Specimens of the test material, including corresponding untreated

controls of the same material, are placed in intimate contact with
growth agar which has been previously streaked with test organism.
After incubation, a clear area of interrupted growth underneath and
along the sides of the test material indicates antibacterial activity of the
specimen.
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Sample Size according to AATCC 147: Rectangular specimens cut 25
- 50 mm They can be any convenient size.

AATCC 147 Test Organism: Gram positive organism :Staphylococcus

aureus, ATCC No. 6538. Gram negative organism: Klebsiella
pneumoniae, ATCC No. 4352.

Other organisms can also be used depending on the intended end-

use.

Turn around Time : 5 to 7 days for commonly used organisms like

mentioned in AATCC 147 test standard.

AATCC 147 Test Method Limitations

The method is not suitable for materials which are not migratory
and are unable to diffuse thru the bacterial growth medium.

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 Name of Test: Assessment of anti-microbial activity by AATCC 147 - 2011
Test Inoculum:
1. Staphylococcus aureus ATCC 6538
2. Klebsiella pneumoniae ATCC 4352
Sample Test Organism Antibacterial Conclusion
Identification activity
Fabric Staph. aureus No zone Growth No antibacterial activity Fails
- Untreated

K. pneumoniae No zone Growth

Saniguard – 7500 Staph. aureus No zone Growth Bacteriostatic Passes

(40 g/l) antibacterial activity
Fabric- - Unwash
K. pneumoniae No zone Growth

Saniguard – 7500 Staph. aureus No zone Growth Bacteriostatic Passes

(40 g/l) Antibacterial activity
Fabric- After 20 wash
K. pneumoniae No zone Growth

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 Acute Oral Toxicity/LD50

One of the most typical toxicity evaluation tests in case of

ingestion. Test method/Using albino rats belong to mouse and
feeding with test samples. The number of fifty percent death
was calculated from the dead rat number statistically

Acute Oral Toxicity/ LD50

Saniguard 7500 12.27 g/kg

Salt 3.0 g/kg
Sorbic acid (food additive agent, disinfectant) 10.5 g/kg
Caffeine (coffee, tea) 0.19 g/kg
Sodium nitrife (a color former, disinfectant) 0.085 g/kg
Ethylacohol (wine, whisky) 14 g/kg

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 SAFETY TEST
Acute Oral Toxicity Albino Rats
Acute Dermal Toxicity Albino Rat
Eye Irritation Albino Rabbits
Primary Skin Irritation Albino Rabbits
Four-Day static Fish Toxicity Studies Rainbow Trout and Bluegills
Human Repeated Insult patch Test Albino Rabbits
28-Day subacute Dermal Toxicity study
Acute vapor Inhalation Toxicity study Albino Rats
Host mediated assay for detection of mutations induced
Three week vaginal irritation study Beagle dogs
32-day human wear test for socks

Three month human wear test for athletic socks

Teratogenic study Albino Rats

Mutagenicity evaluation

In vitro mammalian cell transformation assay in the absence of exogenous

metabolic activation
Acute toxicity Daphnia magna
Percutaneous absorption Rabbits
Eight day dietary Mallard duck
Acute oral toxicity Mallard duck Page 34
Sock wear test/3 month human wear test

Test method:
Socks made of wool, cotton, nylon, acrylic were treated with
Saniguard-7500 and worn by 44 young men, American football
player, for 3-month continuously

Test result:
No observation of skin irritation or skin sensitization

Percutaneous absorption test

Test method:
Backs of rabbit wear clipped free, aqueous solution containing
14C-labeled Saniguard 7500 was applied. The Rabbits were kept
for ten days. 14C contents in urine and feces were analyzed
everyday. After ten- test-day, whole liver, kidneys, lung, spleen,
brain and gonads were removed and 14C content were analyzed.

Test result:
No observation of 14C in urine, faces and all organs. It proved that
Saniguard 7500 was not absorbed from the skin of rabbits.

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Sock (men’s casual, pantyhose, sport, tights)
Underwear (undershirt, underpants, short)
Fillings of bed quilt (polyester, wool, down)
Bedding & night clothes (pajamas, sheet, quilt cover, pillow case, blanket)
Carpet (hospital, School)
Curtain (hospital)
Building walls
Towel (face, hand, bath, sport)
Dish cloth, table towel
Oshibori (steamed towel)
Outer wear (formal suit, casual suit)
Handkerchief
Sport bag
Filter (vacuum cleaner, air cleaner)
Mask
Glove (Kitchen, garden, agriculture & fishing, industry, skiing, golf)
Shoe insole
Shoes, sandals, slippers
Sponge (kitchen, bathroom)
Mat (Kitchen, bathroom)
Toilet seat cover & mat
Shoe keeper
Uniform (working, hospital)
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 TEST METHOD
How to determine if SANIGUARD-7500 is present ?
Bromophenol Blue (BPB) stain test

Soak the treated substrate in BPB for 5 mins (0.04%

solution in Water/ Methanol 80/20 )
Rinse the substrate with copious amount of water
Compare the remaining blue stain on the substrate to the
untreated control and color standard (to be done)

OVERTREATED

SLIGHTLY OVERTREATED
GOOD TREATMENT

SLIGHTLY UNDERTREATED
UNDERTREATED

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 TEST METHODS
What tests show that the groth of bacteria has been prevented ?

Microbiological Laboratory

Microbiological testing is what establishes the baseline

standard that is used to give the BPB (Bromophenol Blue)
analytical procedure its validity for use as a QA and QC tool.
Standard Methods
ASTM - Antimicrobial evaluation testing
AATCC Test Methods (e.g., AATCC 100, AATCC 147, etc.)
AOAC Use Dilution Method for Disinfectants
EPA Germicidal Sanitizer Test

Organisms
• Gram (-) and Gram (+) Bacteria
• Fungi and Yeasts
• Algae

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 SANIGUARD-7500

Anti Microbial Agent for Textile Applications

SANIGUARD-7500 is a stable aqueous solution of an Organosiloxane,

which can produce a durable, non-migrating anti microbial finish to a
wide range of textiles.

PROPERTIES

Appearance Clear to hazy light yellow liquid

Density at 25°C 1.00 g/ml
pH 5±1
Water Solubility Soluble
Boiling Point Approx. 100°C

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SANIGUARD-7500 is water-based
• Contains no heavy metals
• Effective on any fiber and fabric
• Inhibits the growth of Odour causing bacteria.
• Prevents the growth of mould and mildew.
• Eliminates the Odour caused by human sweat on cloth
• Durable up to 50 Laundry treatment
• Effective against Bacteria, Fungi and Dust Mites.
• Falls into the non-Leaching or “bio-static” categories of anti-microbial
agents with lower risk for health and environmental effects
APPLICATION
Mattress pads ad ticking.
• Bed sheets, blankets, and bedspreads.
• Buffer pads (abrasive and polishing).
• Sponges and Polyurethane foam Products Pre-moistened toweling
and tissue wipes
• Athletic and casual shoes, insoles and socks.
• Throw rugs, Toweling
• Upholstery
• Work wear and Uniforms
METHODS OF APPLICATION The product may be applied by
Exhaustion, Padding and Spraying ( for Carpets ) Since we need a specific
quantity of product on the fiber care must be taken in calculating the
amounts for the various applications and Machinery used.
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 BY EXHAUSTION
USE LEVEL :
Depending on the warranty you want to put on the garment or fabric : 3-5%
of SANIGUARD-7500 (on the weight of fabric)
The use of 5-7% gives a reduction rate of 99,99 % after 50 washes.
1. Prior to addition of SANIGUARD-7500 the fabric should be free of any
impurities from other treatments such as Dyeing.
2. Since Alkalinity may prohibit SANIGUARD-7500 from attaching to fabric
the PH of the fabric should be less than 6.0. For optimum exhaustion we
recommend PH 4.5-5.5. Set PH with either Acetic or Citric Acid. If the PH is too
high the fabric will yellow.
3. Set temperature at 40° - 50°C.
4. Pre mix the SANIGUARD-7500 by carefully adding to warm water (25° -
30°C.) Blend for 10 minutes to assure SANIGUARD-7500 is adequately
dispersed in water.
5. Add to the dyeing machine and hold temperature at 40° - 50°C for 15-20
minutes to provide adequate time for exhaustion. At the end of the cycle drop
the bath. No rinsing is required.
6. Fabric can then be dried according to conventional methods. To have a good
polymerization the substrate must be completely dry, all the water must be
removed. Temperatures between 100°C and maximum 210°C can be used.
NOTE: SANIGUARD-7500 can be exhausted on in the softener bath.
Compatibility with Softeners should be checked prior to incorporation into
the recipe.

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 BY PADDING
USE LEVEL :
Depending on the warranty you want to put on the garment or fabric : 30-80
g/l of SANIGUARD-7500 (based on a 100% pick-up)

The use of 80-100 g/l (pick-up 100%) gives a reduction rate of 99,99 % after
50 washes.
1. Fabric should be previously scoured and/or dyed and neutralized.
Alkalinity may prohibit SANIGUARD-7500 from attaching to fabric. pH of
fabric should be less than 6.0 to aid Fixation and avoid yellowing of the fabric.
2. Determine an accurate wet pick up. It is preferable that fabric be padded
wet on dry. However, if wet on wet finishing is necessary, it is imperative than
that an accurate exchange rate will be calculated to effect enough
SANIGUARD-7500 to the fabric.
3. Make up solution of SANIGUARD-7500 as above in header or side tank
near pad. Add solution to pad and run fabric through pad.
4. Check a wet pick up during run to assure correct amount of SANIGUARD-
7500 is being adsorbed on to fabric.
5. Fabric can be dried according to conventional methods. To have a good
polymerization the substrate must be completely dry, all the water must be
removed. Temperatures between 100°C and maximum 210°C can be used.

NOTE: SANIGUARD-7500 can be padded on in the softener bath. Check

compatibility with SANIGUARD-7500 prior to incorporating in to the recipe.

Page 42
 SOME KEY FACTORS
1. ANALYSIS OF APPLICATION OF SANIGUARD-7500

Correct Application of SANIGUARD-7500 can be checked by two

means.
1. Bromophenol. Blue indicator-indicates the presence of
SANIGUARD-7500 by a blue cast. If no SANIGUARD-7500 is
present, no color will form.
2. Phydron quaternary Test Strips QT-10 theses strips indicate the
presence of a quaternary compound. These can be used to
determine the effectiveness of the bath treatment. Use them as you
would pH paper and read the amount of quaternary compound, if
any that has been left in the bath.

2. TO PREVENT YELLOWING OF THE FABRICS WHERE

SANIGUARD-7500 IS USED
To prevent the discoloration or yellowing of fabrics it is necessary
to control the pH of the bath. Alkalinity may prohibit SANIGUARD-
7500 from attaching to fabric. PH of fabric should be 4.5- 5.5 to
aid exhaustion. Different Acids solution can be used for this
process. The most common used are:
• Acetic Acid
• Citric Acid Page 43
FABRIC SHOULD BE CLEAN AND SHOULD BE FREE OF ANY
IMPURITIES FROM OTHER TREATMENTS

ENVIRONMENTAL INFORMATION SANIGUARD-7500 binds

strongly (molecularly) to the fabric and by applying the indicated
quantities an exhaustion or pick-up from 80 – 95% will be
achieved. The amount of SANIGUARD-7500 left in the bath after
the process is a really very low ppm (below the LD 500). So, the
rest bath, after processing, will not affect the sewage or if it goes to
a processing plant will not affect the flora used to treat waters

In case of a spill or a release directly to the sewage pipe the

amount of SANIGUARD-7500 can be deactivated or destroyed by
adding the same amount of an anionic surfactant or an anionic
detergent.

REGULATORY INFORMATION The active molecule used in

SANIGUARD-7500 is registered in the United States by the EPA,
FDA & USDA and this molecule is also listed in European inventory
: EINECS – CAS.

This water-stabilized organosilane compound is patented in the US

since 1999.
Page 44
SAFE USE AND HANDLING
Good hygienic and industrial practices should be followed and, when
employed as recommended, SANIGUARD-7500 will not present any
hazard. However, prolonged skin contact with the neat product
should be avoided and any splashes on the skin should be washed off
with water. For further instructions, see SANIGUARD-7500
Material Safety Data Sheet.

TECHNICAL SERVICE
A team of trained textile technologists, along with full laboratory,
pilot plant and semi-bulk facilities are maintained to carry out any
trials or technical service work associated with our products. This
team of technologists is available to carry out trials at customer’s
premises when appropriate.

These resources are available without any obligation on the customer

to purchase our products.
The information given in this leaflet is based not only on work in
our laboratories but also on the reported results of other
workers in this field. It is offered without guarantee and no
patent liability is assumed.

Page 45
SANIGUARD-7500 - use in Non-textile
CAS Number : 27668-52-6
EINECS : 248-595-8
EPA Number : 75174-

1. Product description
SANIGUARD-7500 is a stable aqueous solution of a Silicone Quaternary
ammonium which can form an invisible and durable micro-biostatic
coating on a broad range of hard surfaces. This water based product is
non-toxic, non-flammable, VOC free and contains no heavy metals
SANIGUARD-7500 possesses powerful anti-microbial properties which
make it very effective against Gram (+) and Gram (-) bacteria and
viruses.
SANIGUARD-7500 is also designed to eliminate a wide range of odors
for extended period of time.
2. Application
Air control : Filter & Air Ducts (metallic materials)
Concrete industry
Paints

3. Product Application
AIR CONTROL : FILTER & AIR DUCTS (metallic materials)
Page 46
SANIGUARD-7500 on FILTER
Provides an invisible durable microbiostatic coating on most
substrates from a water-based system.
Inhibits the growth of odor causing bacteria.
Prevents the growth of mold/mildew and algae.
Is compatible with many processes and conditions, including pH
from 3 to 10.
Attacks malodors at their source to prevent decay.
Can be incorporated into the manufacture of a wide variety of
materials, providing extended life and usefulness to many
products.
Uses
Air purification systems.
Automobiles.
Re-circulating air handling systems.
Vacuum cleaner filters.
Aquariums.

Application level : Generally applied by spray : a 10% dilution

SANIGUARD-7500 on AIR DUCTS (metallic materials)

Page 47
Has 6 months protection against mold, mildew, fungus and algae
Is superior protection from moisture penetration and the effect of weathering
Can be cleaned up easily , and is non-toxic to humans, animals and vegetation
Resists staining and water penetration and even resists spider webbing
Dries to the touch in 1 to 2 hours, may be recoated in 3 to 4 hours depending on
condition

Application level
Spray over air ducts(metalic materials)
Mix thoroughly before use, easily applied with a quality brush, roller, airless
spray and low pressure hand pump sprayer
Always apply material to test panel prior to application
Dry to the touch in 1 ¡V 2 hours, may be recoated in 3 ¡V 4 hours depending
on condition
Dilution Ratio 1 : 5 pure water. 200 litres = 1,000 litres of ready to use(RTU)
products

CONCRETE INDUSTRY
SANIGUARD-7500 in Concrete Industry
Prevents deterioration due to fungi (mold and mildew), bacteria and algae.
Prevents stains caused by fungi (mold and mildew), bacteria and algae.
Prevents odors caused by fungi (mold and mildew), bacteria and algae.
Prevents deposition of dust on the treated surfaces.
Is easy to apply and more effective than traditional antimicrobial products.
Page 48
Uses
Air purification systems.
Automobiles.
Re-circulating air handling systems.
Vacuum cleaner filters.
Aquariums.

Application level
30 ml to 90 ml / litre of water. This amount shall be included in total water of
the concrete mix design (for wet mix shotcrete).

PAINTS INDUSTRY

SANIGUARD-7500 in Paints Industry

LN chemical offer a line of EPA-registered antimicrobial products approved to
be used in wood , decks painting, latex indoor and outdoor paints and stains;
woodstains, architectural paints; lacquer and maintenance coatings; films;
laminates and finishes including alkyd, urethane, enamel, epoxy, siloxaline,
amino resins, textile coatings, extrusion coatings, architectural coatings and
overlays, anti-corrosion coatings fire-resistant coatings, aliphatic coatings,
vinylester and polyester coatings, gel coatings, aminoresins, resins used as
additive mixes for cement, epoxy laminating resins.

By incorporating SANIGUARD-7500 in the Wood for wood furniture will

inhibit deterioration and damage cause by these microorganisms for an
extended period of time.
Page 49
Uses

LN Chemical antimicrobials products were designed for

applications that demand high levels of prevention of Mold &
Mildew including:
Wood Furniture
Industrial Building
Homes & Apartments
Long-term Care Facilities
Hotels & Motels
Offices & Conference Centers
Supermarkets & Restaurants
Food Processing Centers
Doctors Offices
Government Buildings
Laboratories & Research Centers
School & other Public Buildings
Airports & Transportation Centers
Commercial & Private Kitchens
Public & Private Washrooms

Page 50
SAFE USE AND HANDLING
Good hygienic and industrial practices should be followed and, when
employed as recommended, SANIGUARD-7500 will not present any
hazard. However, prolonged skin contact with the neat product should
be avoided and any splashes on the skin should be washed off with
water.

For further instructions, see SANIGUARD-7500 Material Safety Data

Sheet.

TECHNICAL SERVICE
A team of trained textile technologists, along with full laboratory, pilot
plant and semi-bulk facilities are maintained to carry out any trials or
technical service work associated with our products. This team of
technologists is available to carry out trials at customer’s premises
when appropriate.

These resources are available without any obligation on the customer

to purchase our products.

The information given in this leaflet is based not only on work in

our laboratories but also on the reported results of other workers
in this field. It is offered without guarantee and no patent liability
is assumed.

Page 51
Gram Negative Bacteria
Acinetobacter calcoaceticus 2, 5, 6, 11, 14, 15
Aeromonas hydrophilia 5, 6, 11
Citrobacter deversus 5, 6, 11
Citrobacter freundi 5, 6, 11
Enterobacter aerogenes 5, 6, 7, 11
Enterobacter aglomerans 2, 5, 14, 15
Enterobacter cloacae 5, 6, 7, 11
Enterococcus 10
Escherichia coli 1, 2, 3, 5, 6, 7, 10, 11, 13, 14
Klebsiella oxytoca 5, 6, 11, 14
Klebsiella pneumoniae 3, 5, 6, 7, 9, 10, 11, 13, 14
Klebsiella terriena 19
Legionella pneumophila 1
Morganella morganii 5, 6, 7, 11
Proteus mirabilis 5, 6, 7, 11
Proteus vulgaris 5, 6, 7, 11
Pseudomonas aeruginosa 2, 3, 5, 6, 7, 11, 13, 14
Pseudomonas fluorscens 5, 6, 7, 10, 11
Salmonella cholera suis 5, 6, 7, 11, 14
Salmonella typhi 5, 6, 7, 11, 14
Salmonella typhimunium 1, 5, 6, 7, 11
Serratia liquifaciens 5, 6, 7, 11
Serratia marcescens 5, 6, 7, 11
Xanthomonas campestris 5, 6, 7, 11
Page 52
Viruses
Adenovirus Type II & IV 17, 18, 21
Bovine Adenovirus Type I & IV 17, 18, 21
Feline pneumonitis 21
Herpes Simplex Type I 16, 17, 18
Herpes Simplex Type II 21
HIV-1 (AIDS) 21
Influenza A2 (Aichi) 17, 18, 21
Influenza A2 (Asian) 17, 18
Influenza B 17, 18
Mumps 17, 18
Parinfluenza (Sendai) 21
Rous Sarcoma 17, 18
Reovirus Type I 17, 18
Simian Virus 40 17, 18
Vaccinia 17, 18
MS2 19
PRD1 19

Page 53
Saniguard-7500
The following list outlines the bacteria, virus,
fungi, algae, molds, and yeasts
Saniguard-7500 has been shown to be active
against and helps eliminate.
Gram Positive Bacteria
Bacillus sp. (vegetative cell) [5, 6, 11]
Clostridium difficule 25
Corynebacterium diptheriae 1, 13
Listeria monocytogenes 24, 25
Micrococcus lutea 5, 6, 11
Micrococcus sp. 2, 5, 15
Mycobacterium tuberculosis 14
Mycobacterium smegmatis 14
Propionibacterium acnes 5
Staphylococcus aureus 2, 3, 5, 6, 10, 11, 13, 24,
15, 21
Staphylococcus aureus (MRSA) 25
Staphylococcus epidermidis 2, 5, 6, 7, 11, 13, 14,
15
Streptococcus faecalis 2, 5, 6, 7, 11, 13, 14
Streptococcus mutans 5, 6, 7, 11
Streptococcus pneumonia 1
Streptococcus pyogenes 5, 6, 7, 11 Page 54
Fungi, Algae, Mold, Yeast, Spores
Alterania alternate 8,12
Aphanizomenon sp. 22
Aspergillus flavus 2, 5, 6, 7, 11, 14
Aspergillus niger 2, 5, 6, 7, 8, 11, 12, 13, 14
Aspergillus sydowi 5, 6, 7, 11
Aspergillus terreus 5, 6, 7, 11, 14
Aspergillus versicolor 2, 5, 6, 7, 11
Aspergillus verrucaria 14
Aureobasidium pullans 5, 6, 7, 8, 11, 12
Candida albicans 1, 2, 5, 6, 7, 14
Candida pseudotropocalis 5, 6, 7, 11
Chaetomium globsum 1
Cladosporium cladosporioides 8, 12
Chlorella vulgaris 19
Dreschslera australiensis 8,12
Epidermophyton sp. 9
Gliomastix cerealis 8, 12
Gloeophyllum trabeum 5, 6, 7, 11
Microsporum sp. 9
Microsporum audouinii 21
Page 55
Monilia grisea 8, 12
Oscillatoria 20
Penicillium chrysogenum 5, 6, 7, 11
Pencillium commune 8, 12
Penicillium funiculosum 1, 2, 5, 6, 7, 11, 14
Penicillium pinophilium 5, 6, 7, 11
Penicillium variable 5, 6, 7, 11, 14
Phoma fimeti 8, 12
Pithomyces chartarum 8, 12
Poria placenta 5, 6, 7, 11
Scenedesmus 20
Saccharonyces cerevisiae 5, 6, 7, 11, 13, 14
Scolecobasidium humicola 8, 12
Selenastrum sp. 22
Trichoderma viride 5, 6, 7, 11
Trichophyton interdigitale 2, 14
Trichophyton maidson 14
Trichophyton mentogrophytes 5, 6, 7, 9, 11
Trichophyton sp. 9
Protozoa Parasites
Cryptosporidium parvum (oocysts) 19

Page 56
 UNTREATED
LEACHABLE SAMPLE
Leaches out of substrate
to inhibit growth

SAMPLE OF
LEACHING
DURABLE
ANTIMICROBIAL
Saniguard-7500
Durably bonded solid*
that inhibits growth by
physical contact
* The solid is a micro polymer BONDED
network ANTIMICROBIAL

Page 57
 EXAMPLES OF THE Saniguard-7500
ORGANO SILANE SYSTEM AT WORK ?

BEFORE AND AFTER STAPH

Staph Aureus Staph Aureus after

bacteria before an Saniguard-7500
treatment Organo
Silane treatment

Page 58
 BEFORE AND AFTER E. COLI

E. coli bacteria E. coli bacteria after

before treatment an saniguard-7500 Organo
Silane treatment

Page 59
 Fresh ideas to keep socks fresh and odour free

This patio furniture was treated with 2% SANIGUARD-7500 and

exposed to southeastern weather conditions for a period of one
year

Saniguard-7500

competitor « hot and humid » country

Saniguard-7500
Page 60
Page 61
Page 62

OFFICE ADDRESS :
403, “Antariksh”, Makwana Road,
Marol Naka, Andheri (E),
Mumbai - 400 059
Tel.- 91-22-4221 6789 (30 Lines)
91-22-6692 4112
Fax -91-22-2836 6434
Email ID: ketan@lnchem.in
nayana.lnchem@gmail.com
FACTORY ADDRESS :
D- 14 /10, TTC, MIDC Industrial Area,
Turbhe, P. O. K. U. Bazar,
Navi Mumbai - 400 705
Tel.-91- 22-4141 9899 (100 Lines)
Fax -91-22-4141 9888
INTEGRATED MANAGEMENT SYSTEM (IMS)
CERTIFIED: ISO 9001 : 2008 , ISO 14001: 2004,
ISO 18001 : 2007
GLOBAL REPORTING INITIATIVE GRI-G4
CERTIFIED CRISIL RATING : SE-1A
Page 63