ICUMSA Method of the determination of White Sugar Solution Colour 1 Scope ‘This method is used for the determination of white sugar solution colour in sugars not exceeding 65 LU, a5 determined by Method GS23-9." [NOTE This methodist be profeed thot of Method GS23-9 for sia sous rth eave ene oft eplying TEA 2 Field of Application ‘The method can be applied to all crystalline or pow- dered white sugars and very pure syrups (General Sub- Jects 2 and 3), provided that a filtered test solution can bbe prepared by the procedure specified in the method, ‘The method is not suitable for those sugars which con- tain colouring matter, turbidity or additives to an extent that filtration is not practical 3 Definitions’ 31 ‘Transmitance ofa soltion, IZ reeset the rin energy inet pone et see ofthe iSden suYoreses ths ata en lenin te trond ve te slin Then Tro fa = ansnitance ofthe sain (1007-= percentage transmittance) 3 Tenens. Lat, pc he ected ensling aban alla eee ‘sent the transmittance of the same or duplicate cell con- a dae kere Ten Jee vanity ft ction Te 33. Absorbaney (extinction). Then: A, =~log,T;= absorbancy of the solution 34 Absorbaney index (extinction index). Let b rep- resent the lengtb, (om), of the absorbing path between the boundary layers of the solurion and let ¢ represent the concentration, (g/ml), ofthe sugar solution. Then: anes = esrb index of te slion 3S ICUMSA Colour. The value of the absorbancy in- ‘dex multiplied by 1000 is reported as [CUMSA Colour. ‘The sesulting values aze designated as ICUMSA Units Uy. 4° Principle White sugar is dissolved in distilled water to give a 50 4% sugar solution. ‘The solution is filtered through a membrane filter to 1e- ‘move turbidity, The absorbency of the filtered solution is measured at a wavelength of 420 nm and the sol colour is calculated. 5 Reagents Use only distilled water or water of equivalent purity. 6 Apparatus 6 Instrument. Spectrophotometer or colorimeter ceapable of light ansmission measurements at 2 wave- length of 420 nm with the narrowest practical band- width, eg. + 10 nm. The instrument should be fired with a grating, prism or interference filter monochro- ator. [NOTE ~ The sutabily ofthe tnstrumeot fortis spetal purpose ‘oul be tested using andar sugar wih known certified colour ‘Socn standard suger may be cbiited frou lnsut fr Teckoologie er Koblenlydrate~ Zuckeinstcat eV. ~ Langec Ker 5.D-28105 ‘Braunschweig. Gesmany. 62 Associated optical cells. Use a coll of at least 4 cem in length. A cell length of 10 cm or more is to be preferred for low colour white sugars. A second or ref- erence cell may be used, provided that a test with dis- tilled water has shown that the two cells are within 0.2% of being identical. 63 Membrane filters ~ pore size 0.45 jam, diameter ‘50 mum, preferably of cellulose nitrate material. [NOTE ~ Pore sie at deteined by ‘bubbte pint testing. 644 Membrane filter holder - preferably fitted with a stainless steel support 65 Vacuum oven, vacuum desiceator or ultrasonic ‘bath - for de-acration of the filtered sugar solution 66 Refractometer. 6.7 Laboratory balance ~ readable to 0.1 7 Procedure 7A Sample preparation. Mix the sample of suger thoroughly. Weigh 50.0 40.t g of the sample into a 250 rm. conical flask, add 50,0: 0.1 g of distilled water (5) tnd dissolve the sugar by swirling at room temperatureFilter the sample solution under vacuum through 3 membrane filter (6.3) into a clean, dry conical fiask De-aerate the filtered solution for I hour at room tem perature in a vacuum oven or sn evacuated desiccator. Alternatively de-aerate by immersing the conical flask, ing the suger solution, in an ultrasonic beth for Measure the sefractometric dry substance (RDS) of the solution, 0 an accuracy of + 0.1 g/100 g, by the ICUM- SA method? as also described in Method GS4-13, 7.2 Colour measurement, Set up the colour measur ing instrument (6.1) according to the manufacturers in- structions and adjust the wavelength to 420 nm. Rinse the measuring cell with sugar solution and then fill. Determine the absorbancy (A, or -log,,77) of the solu- tion using filtered de-acrated distilled water as the ref- ‘erence standard for zero colour, 8 Expression of Results 8.1 Calculation. Calculate the concentration of sam- pile solids in solution, c, from the RDS measured in 7.1. Use the RDS to obtain the density p in kg/m ofthe test solution, from Table 1 by interpolation, the appropriate ICUMSA Table in SPS-4 or the relevant equation’, ‘Then the concentration of the test solution is given by: Saye amt 0 Tabet % RDS Density kgm) 7 12133 8 12187 % 12242 50 1229.7 St 1235.2 2 12407 323 From the definition given in 3.5: 10004, ICUMSA Colour = be 4, “1 Express results to the nearest whole number. [NOTE — When using SPS Tables, strcly peaking the date for ‘n\W ehoald be taken, oot data for 9. An eror ofthe order of only (14, however is introduced by sing data for p NOTE ~ THE CHOICE OF METHOD, WHETHER 6523.9, (G52/3-10 OR CS1/3-7, MUST BE STATED WITH THE RESULTS. 8.2. Precision, For sugars with ICUMSA Colour val- ues up to SO TU, the absolute difference between two results, obtained under repeatability conditions, should. not be greater than 3 1U. For sugars with ICUMSA Col- ‘our values up to 50 10, the absolute difference between two results, obtained under reproducibility conditions, should not be greater than 7 IU. 9 Bibliography 1. Pros, 23rd Session ICUMSA, 2002, 77, 111 2. Proc, 22nd Session ICUMSA, 1998, 258 3. Schneider F, ed. (1979): Sugar Analysis: ICUMSA. Methods, 125-126 Millipore Laboratory Catalogue (1991): Millipore Intertech, Bedford, Mass, 9 5. Schneider F, ed. (1979): Sugar Analysis: ICUMSA Methods, 120-121 6. Proc. 20th Session ICUMSA, 1990, 267-268 1. Proc. 22nd Session ICUMSA, 1998, 259-276