MGI NEXT GENERATION SEQUENCING (NGS) DNBSEQ-G400RS

Research series (Free Maintenance 5 years)

Spesifikasi:
Basic Parameter
Dimensions : 1086mm×756mm×710mm
Net Weight : 200 Kg
Power : 100-240V，1200VA
Screen : 20-inch touch screen, resolution 1920×1080
Operating Environtment Requirements
Temperature : 19℃-25℃
Relative Humidity : 20% RH-80% RH
Atmospheric Pressure : 70 kPa-106 kPa
Max. Attitude : 3000 m
Rated Pollution Degree : Level 2
Control Computer Configuration
CPU : Intel Xeon E5 10Core * 2 2.2GHz
Internal Storage : 256GB RAM
HDD : 16 Tb
SSD : 480 Gb
Software Environtment
Pre-installed Software : Microsoft Windows 10 64-bit operating system, Microsoft .Net
Framework 4.6.1, control software
Atmospheric Pressure : C/S
Network Type : LAN
Network Broad Band : Not Lower than 1 Gbit/s
Data and Instrument Interface
Network Socket : 2 (internet connection)
USB : 4 (keyboard, mouse, barcode scanner, or for standby application)
Functions =
Range : This product can complete DNB loading on the Flow Cell, sample sequence information
collection, identification and preliminary analysis of sequence information

Input & Output : The input is the sequencing reagent, Flow Cell and user-prepared DNB library
produced by MGI. The output is FASTQ file and sequencing quality report

Core Principle : The core principle is MGI's DNBSEQ™ technology, including DNA singlestrand
cyclization & DNB preparation, Patterned Array Flow Cell, DNB loading, cPAS (combinatorial
Probe Anchor Synthesis), Pair-End sequencing technology, and matched fluid, optical detection
techniques and base calling algorithms

User Experience : This product has power-on self-test, status monitoring, automatic alarm, log
management, user authorization management, multi-language operation interface,
consumables identification, process guidance, sequencing customization, data security, data
cleaning, cloud data transmission, parameters calibration, common communication interfaces
and other functions that are beneficial to the user experience
Technological Advantages
Increased Accuracy : No PCR amplification required. Our unique Rolling Circle Amplification
(RCA) technology employed in DNBSEQ™ library construction eliminates errors associated with
PCR. Only the original template DNA is used to generate copies and therefore amplification
errors do not accumulate, resulting in greater accuracy for detection of significant mutations
such as Indel detection

Decreased Duplicates : This product utilizes Patterned Array technology that produce Flow Cell
active spots with the size identical to DNB. This ensures that only a single DNB is attached at
each spot and therefore contributes to greater saturation of DNB on the Flow Cell with
unprecedented uniformity. This technology enables an industry-leading detection capability and
an average duplicate rate below 3%.

Low Index Hoping : MGI platform’s unique library prep and RCA results in much lower index
hopping rates compared with other platforms, at a rate of only 0.0001%~0.0004%

Performances
Flow Cell Number : The sequencer supports single flow cell and dual flow cells modes

Flow Cell Type : The sequencer supports 2 flow cell types (FCS and FCL).
FCS: Single flow cell effective reads are 550M, with totally 2 lanes (different samples can be
loaded to different lanes).
FCL: Single flow cell effective reads are 1500M-1800M, with totally 4 lanes (different samples can
be loaded to different lanes).

Sequencing Method : Supports Single-End (SE) sequencing and Pair-End (PE) sequencing
methods

Read Lengths : The product supports SE50, SE100, SE400, PE100, PE150, PE200

Throughput : 2 FCS can generate up to 330G data in PE150 mode per run; 2 FCL can generate up
to 1440G data in PE200 mode per run

Run Time : FCL PE150 sequencing at full capacity in 66 hours while FCS PE150 sequencing at full
capacity in only 37 hours

Data Quality : When read length ≤200 (including SE50, SE100, PE100), Q30＞85%. The
sequencing quality decreases as the read length increases

Installations & Maintenance

This product has good installability, easy to install and debug on site, and one person can
complete it independently.
This product has good maintainability, short daily maintenance time and easy maintenance
process.
This product has good maintainability, remote fault diagnosis capability, and easy replacement
of parts.
Included:
MegaBOLT Bioinformatics Analysis Accelerator,
Invitrogen Qubit 4.0 Fluorometer,
UPS ICA 3.2 kVA (for G400 RS),
UPS ICA 1.6 kVA (for MegaBOLT),
Micropipette set (for sample prep and Library prep),
Rongke CPU (Intel Xeon E5 10Core * 2 2.2GHz, Internal Storage : 256GB RAM, HDD : 16 Tb, SSD
: 480 Gb),

Free maintenance selama 5 tahun, dengan rincian sebagai berikut :

1) Dilakukan Preventive Maintenance (PM) Visit setahun sekali dalam jangka waktu 5 tahun.
Pekerjaan PM meliputi pembersihan alat, pengecekan part unit, pelumasan komponen
mekanik, pengecekan workflow dengan script, Update Software, penggantian part PM
seperti : TriContinent Syringe, tubing/selang, Sealing gasket & Air Filter;
2) Penggantian part unit jika terjadi kerusakan dalam jangka waktu 5 tahun;
3) Remote Online Support;
4) OnSite Support Visit jika diperlukan (saat terjadi kendala teknis)