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Drug Analysis (II) lab report

SUBMITTED TO: PROF. Rita Morigi

SUBMITTED BY: Hamid Abi
Detection Procedure:
As the unknown sample was a solid compound, first, the combustion assay was
carried out to detect whether it was an organic, inorganic or an organometallic
substance. Hence, upon the conduction of this assay, as the complete burning of
the substance was observed, and it completely turned into ashes, it came to light
that it a pure organic substance.
Proceeding to the next step, knowing the substance contained N and S, it had to
be checked whether the substance belonged to primary aromatic amines,
hydrochlorides, sulfates or other compounds, therefore, the test of solubility was
carried out. Initially, solubility in water was checked and since it was insoluble in
water, next, its solubility in dilute NaOH was checked and it was confirmed that it
was soluble in dilute NaOH.
Following this step, by referring to page 712 of Pharmacopeia, in order to detect
the substance from the list in the page, assays of melting point and IR were
conducted. First, the substance’s melting point was discovered to be almost 258
degrees, and then, its IR analysis confirmed the right corresponding wavelengths
of the substance Acetazolamide. By having these two determining parameters in
hand, the unknown sample was confirmed to be Acetazolamide.